LOCUS       DAVBHS010000001        44932 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVBHS010000001 DAVBHS010000000
VERSION     DAVBHS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.001"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            264..932
                     /locus_tag="VQ651_000001"
     CDS             264..932
                     /locus_tag="VQ651_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2753066.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            892..1569
                     /locus_tag="VQ651_000002"
     CDS             892..1569
                     /locus_tag="VQ651_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2753067.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIEISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLDFTRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GAAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            1570..2327
                     /locus_tag="VQ651_000003"
                     /pseudo
     CDS             1570..2327
                     /locus_tag="VQ651_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            2339..3200
                     /locus_tag="VQ651_000004"
                     /pseudo
     CDS             2339..3200
                     /locus_tag="VQ651_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(3280..3927)
                     /locus_tag="VQ651_000005"
     CDS             complement(3280..3927)
                     /locus_tag="VQ651_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2753068.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(3924..4622)
                     /locus_tag="VQ651_000006"
     CDS             complement(3924..4622)
                     /locus_tag="VQ651_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753069.1"
                     /translation="MGKLSSHMWRLNKIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDVGGDLFLQLLFLVFLFQ
                     SIFYFTRQKRRFIE"
     gene            complement(4632..5384)
                     /locus_tag="VQ651_000007"
     CDS             complement(4632..5384)
                     /locus_tag="VQ651_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753070.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(5384..6148)
                     /locus_tag="VQ651_000008"
     CDS             complement(5384..6148)
                     /locus_tag="VQ651_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753071.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(6244..6647)
                     /locus_tag="VQ651_000009"
                     /pseudo
     CDS             complement(6244..6647)
                     /locus_tag="VQ651_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6827..7459)
                     /locus_tag="VQ651_000010"
     CDS             complement(6827..7459)
                     /locus_tag="VQ651_000010"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET2753072.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYRELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(7493..8194)
                     /gene="pyrF"
                     /locus_tag="VQ651_000011"
     CDS             complement(7493..8194)
                     /gene="pyrF"
                     /locus_tag="VQ651_000011"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET2753073.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQNWN"
     gene            8515..8607
                     /locus_tag="VQ651_000012"
                     /pseudo
     CDS             8515..8607
                     /locus_tag="VQ651_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(8929..9642)
                     /locus_tag="VQ651_000013"
     CDS             complement(8929..9642)
                     /locus_tag="VQ651_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2753074.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNKTLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(9635..10330)
                     /locus_tag="VQ651_000014"
     CDS             complement(9635..10330)
                     /locus_tag="VQ651_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2753075.1"
                     /translation="MTGQFLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYEKMS
                     LYNNLLFYSKFYNISKSRVDNLLKRVGLYDSRKMVAGKLSTGMRQRMLLARALINKPA
                     VLFLDEPTSGLDPTTSRTIHELILELKTAGTTIFLTTHDMNEATLLCDYVALLNKGKL
                     VEQGAPSELIQRYNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLE
                     DIFITLTGGKLNA"
     gene            complement(10670..12002)
                     /locus_tag="VQ651_000015"
                     /pseudo
     CDS             complement(10670..12002)
                     /locus_tag="VQ651_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812020.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
     gene            complement(11986..12784)
                     /locus_tag="VQ651_000016"
                     /pseudo
     CDS             complement(11986..12784)
                     /locus_tag="VQ651_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50 family protein"
     gene            complement(13574..14773)
                     /gene="divIB"
                     /locus_tag="VQ651_000017"
     CDS             complement(13574..14773)
                     /gene="divIB"
                     /locus_tag="VQ651_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HET2753076.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLSKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            complement(14783..15841)
                     /locus_tag="VQ651_000018"
     CDS             complement(14783..15841)
                     /locus_tag="VQ651_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HET2753077.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVCWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VAKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQLENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(15843..17195)
                     /locus_tag="VQ651_000019"
     CDS             complement(15843..17195)
                     /locus_tag="VQ651_000019"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET2753078.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(17299..17940)
                     /locus_tag="VQ651_000020"
     CDS             complement(17299..17940)
                     /locus_tag="VQ651_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2753079.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTFL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTTSLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(17942..19963)
                     /locus_tag="VQ651_000021"
     CDS             complement(17942..19963)
                     /locus_tag="VQ651_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2753080.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLMSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(20034..20333)
                     /locus_tag="VQ651_000022"
     CDS             complement(20034..20333)
                     /locus_tag="VQ651_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2753081.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSLVSRWNSHSDKGEASAGKTSYAWIWTKWGEQVAFYCDYD"
     gene            complement(20699..20953)
                     /locus_tag="VQ651_000023"
     CDS             complement(20699..20953)
                     /locus_tag="VQ651_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HET2753082.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(20973..22814)
                     /gene="typA"
                     /locus_tag="VQ651_000024"
     CDS             complement(20973..22814)
                     /gene="typA"
                     /locus_tag="VQ651_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET2753083.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            22976..23701
                     /locus_tag="VQ651_000025"
     CDS             22976..23701
                     /locus_tag="VQ651_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HET2753084.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVQVNELLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            complement(23741..23968)
                     /locus_tag="VQ651_000026"
     CDS             complement(23741..23968)
                     /locus_tag="VQ651_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753085.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(24057..24437)
                     /locus_tag="VQ651_000027"
     CDS             complement(24057..24437)
                     /locus_tag="VQ651_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2753086.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            complement(24437..24706)
                     /locus_tag="VQ651_000028"
     CDS             complement(24437..24706)
                     /locus_tag="VQ651_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HET2753087.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            24770..25678
                     /locus_tag="VQ651_000029"
     CDS             24770..25678
                     /locus_tag="VQ651_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET2753088.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            complement(25732..26487)
                     /locus_tag="VQ651_000030"
     CDS             complement(25732..26487)
                     /locus_tag="VQ651_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET2753089.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(26508..27437)
                     /locus_tag="VQ651_000031"
     CDS             complement(26508..27437)
                     /locus_tag="VQ651_000031"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET2753090.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            complement(27452..28075)
                     /locus_tag="VQ651_000032"
     CDS             complement(27452..28075)
                     /locus_tag="VQ651_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HET2753091.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            complement(28068..29306)
                     /gene="hflX"
                     /locus_tag="VQ651_000033"
     CDS             complement(28068..29306)
                     /gene="hflX"
                     /locus_tag="VQ651_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET2753092.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            complement(29299..30183)
                     /gene="miaA"
                     /locus_tag="VQ651_000034"
     CDS             complement(29299..30183)
                     /gene="miaA"
                     /locus_tag="VQ651_000034"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET2753093.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKCFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            30304..30474
                     /locus_tag="VQ651_000035"
     CDS             30304..30474
                     /locus_tag="VQ651_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HET2753094.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            complement(30522..31361)
                     /locus_tag="VQ651_000036"
     CDS             complement(30522..31361)
                     /locus_tag="VQ651_000036"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET2753095.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPIITLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(31458..32417)
                     /locus_tag="VQ651_000037"
     CDS             complement(31458..32417)
                     /locus_tag="VQ651_000037"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HET2753096.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENTFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            complement(32544..33542)
                     /locus_tag="VQ651_000038"
     CDS             complement(32544..33542)
                     /locus_tag="VQ651_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2753097.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DLHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQAATTQTDTTSSTASGWVKQDGS
                     WYYFDGNGNLVKNAWQGNYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTA
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDG"
     gene            complement(33592..34209)
                     /locus_tag="VQ651_000039"
     CDS             complement(33592..34209)
                     /locus_tag="VQ651_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2753098.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            complement(34210..35931)
                     /gene="pabB"
                     /locus_tag="VQ651_000040"
     CDS             complement(34210..35931)
                     /gene="pabB"
                     /locus_tag="VQ651_000040"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HET2753099.1"
                     /translation="MHRKTVIDFRTLGERYTFAQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            complement(36000..41687)
                     /locus_tag="VQ651_000041"
     CDS             complement(36000..41687)
                     /locus_tag="VQ651_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2753100.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEETPKEEAPRTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKSEEKVAVKPESQPSDKPAEES
                     KVEPPVEQAKVPEQPVQPTQAEQPSTPKESSQEDNPKEDRGAEETPKQEESTPDTKAE
                     ETVEPKEETKTAKGTQEEDYVGEALVPEQLPEYKVTEGTLVEESTTELDYKTETTEDP
                     TKYTDEETVLRNGEKGSQVTKTTYKTIGGVKTDQVLSTSTEVTKEPVNQQVIRGTKPI
                     EGTTVETREEIIPFETKEQEDDTLKRGTRQVTQEGVNDKKQITETYKTIRGEKTSDAP
                     TITETVIEKPQDKIIKNGTKELEKPTLEWAKTDKDVLKKSATASYTLTKPAGVEIKSI
                     KVALKDNTGTVVKEVTVEENNLQATLDNLKYYQGYTLSTTMVYDRGNGEETEILEDKE
                     VQLDLKKVEIKDIKETRLMKVDENGNETDSSLLETVPENLTSYYLKITTNHNKVTRLA
                     ITNIEEVTEEETSLFKVTASAPDLIQRDSNNSLSQTYTYYIEKPKASQNNVYYNFKDL
                     VDAMQKNPNGEFKLGSDLNATNVPTPSKSYVTGKFTGHLTSVDGKHFSIHNTARPLFD
                     RVEGGSIKNINLINVNINMPWANQLASLAQTVKNATVENIKVTGTIVGNNNVAGILDK
                     LDDAGIMTNVAFIGKVQSVGDKGWNLAGIASEIYRGNLNKAYADVELKGLNTRAGAIV
                     GHLAHDFNPRDVGAWSNVRNSVAKGTIDVARGDVGGLIGKDWPWGTIDNNISLVNVKR
                     GDIFFGSNDMDDEFVSNWIKPNNATVIGVSSGDKTHKQSSKIKEISQVEADKKIKDMG
                     ITADTFEVTEHLVDKLNRRILRDEEYKTIQDYNANRELVYRNVEKLQPFYNKEWIVNQ
                     GNKLTTDSPLMNKEVLSVTAMKGNDFITDLTDADHIMVHYADKTKDIFTISPKDSQVK
                     QVKEYSVAELGEVVYTPNMVVKDRADLISAIEGILSPIELQSDPIYQRLGRTGDNRVN
                     AIKNLYLEESFKEVKDHLNTFVTKLVENEDHQLNTDEAAKRALIKKIEDNKAAVLLGL
                     TYLNRYYGVKFDNFNIKELMLFKPDFYGKNVSVLDRLIEIGSKEDYIKGSRTQESFRE
                     VVAKNTLSGNLHDFLTYNMKLFTNETDINVWFKKAIENNAYVVEQPSTNPAFANKKYR
                     LYEGLNNGQHGRMILSLLNLKDAHLFMISTYNTISFSSFEKYGKDTEEKRESFKSEIN
                     KRAKEQVNYLDFWSRLATDNVRDKLLKSQNVVPTPVWDNHGAPGGWPDRFGHRKDKPD
                     YAPIREFFGRIDKYHDYKYGYGAYAYIFADPQPMDAVYFVMSDLISEYGTSAFTHETT
                     HVNDRMAYLGGHRHRQGTDLEAFAQGMLQTPAEHGHQGEYGALGLNMAFERSNDGNQW
                     YNPDPTKLQTRDQIDHYMKNYNEAMMMLDYAEAEAVLPKVKGDNSKWFKKIDRETRRP
                     MDRNKLSGPHQWDKVRDLIDAEKTTNLETIDDLVNNNFMTIHGNPGNKVFHPEDFGTA
                     YVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGYYGYEHGFIDYVSSKHQGAANKEN
                     NGLLGDDFIIKKVSNNQFSNLEDWKKQWYGEVLTKSKKGFAPIEINGTQISTYEQLKT
                     MFDEAVTKDLTELNDSSIKDKYRNTVVLKSKVFKALLKNTDGFSGDLFTAPQA"
     gene            complement(41709..42272)
                     /locus_tag="VQ651_000042"
     CDS             complement(41709..42272)
                     /locus_tag="VQ651_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HET2753101.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGVLFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            complement(42388..44079)
                     /locus_tag="VQ651_000043"
     CDS             complement(42388..44079)
                     /locus_tag="VQ651_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2753102.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            complement(44076..44813)
                     /locus_tag="VQ651_000044"
     CDS             complement(44076..44813)
                     /locus_tag="VQ651_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2753103.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
BASE COUNT        13550 a         9702 c         7775 g        13905 t
ORIGIN      
        1 tctacatgac cttctgcatt gcttgttttg tatgacataa aatgtcctcc ttaataagta
       61 agcgtttact ttgtgtatta caaaaatatc ttaactcttt ttcaaaactt ttaaaatatt
      121 ttgtttggaa atttcagaaa ttttatgtct atgataaaaa tccttataac ggcaataaaa
      181 aatagatatt atccaaagaa gattttaagt gctacaataa ctgtattatt tctagatggg
      241 aggttctatt tttggattga tccattgttg aacaatatct accactatat caaaaggcat
      301 tctttctgac cttgcatatt gcagtttggg gaattttggg atcctttctg ctcggtttaa
      361 tcgttagtat catccgacat tatcgaatcc ttgttttggc gcaagtagcg acagcctaca
      421 ttgaattgtc acgtaatacg ccccttttga ttcaactctt ctttctctac ttcggtcttc
      481 cccgaatcgg gattgtccta tcttcagaag tctgtgcaac gcttgggctt gtctttttag
      541 gaggctccta tatggcagaa tctttccgaa gtgggctgga agccatcagt caaacccagc
      601 aggagattgg cctcgctatt ggtctgacac ctctacaggt cttttactat gtggttcttc
      661 cgcaagcaac agcggtggca ctcccctcct ttagtgccaa tgtcattttc cttatcaagg
      721 aaacctctgt tttctcagca gtggctttgg ccgacctcat gtacgtcgcc aaggatttga
      781 ttggtctcta ctatgagaca gacattgcgc tagctatgtt ggtagttgct tatctgatca
      841 tgctgctacc catctcactg gtctttagct ggatagaaag gaggctccgc catgcaggat
      901 tcgggaatcc aagtactctt tcaaggaaat aatctcctga gaatcttaca gggattgggc
      961 gttacgattg agatatccat cctgtctgtc ctcttatcca tgatgttcgg aacagtcatg
     1021 ggaatcatca tgacctccca ttctagaatc atacgatttt taacacgatt gtatctggac
     1081 tttacccgta tcatgcccca gctggtgcta ctcttcatcg tttactttgg cttagctcga
     1141 aactttaata tcaatatctc aggtgagact tcagctatta tcgtttttac cctctgggga
     1201 gcagctgaaa tgggagactt ggtacgtgga gctatcactt ctctccctaa acatcagttt
     1261 gaaagtggac aggcactcgg cttgactaat gttcaacttt actaccacat catcatccca
     1321 caagtcttaa gaagactgct accgcaggct atcaatcttg tcactcggat gattaaaacc
     1381 acttcattag ttgttttgat tggggttgtg gaagtgacca aagttggaca acaaatcatc
     1441 gatagcaatc gcctgaccat cccaactgct tcattttgga tttatggaac cattctagtc
     1501 ttatatttcg cagtttgcta ccctatttcc aaactatcca ctcacttaga aaaacattgg
     1561 agaaactaaa tgtctgaaac tatcttagaa atcaaggaac taaaaaaatc cttcggagac
     1621 aatcccatcc tccaaggact ttctctagaa atcaaaaaag ggaagttgtt gtcatcctag
     1681 ggccatctgg ttgtgggaaa agtaccctcc ttcgttgcct caacggctta gaaagtattc
     1741 aaggtggaga tattcttctg gatggtcagt ctatcgttga aaataaaaaa gattttcacc
     1801 tagttcgcca aaagattggc atggtctttc aaagttatga actctttccc catctggatg
     1861 tcttacaaaa cctcatccta ggccctatca aagctcaagg aagggacaag aaagaagtaa
     1921 cggaagaagc tttgcaatta ctagagcgtg tcggtttgct ggataaacaa catagctttg
     1981 cccgtcaatt atctggtgga cagaagcaac gtgttgcaat tgtccgtgcc ctcctaatgc
     2041 atccagaaat catccttttt gacgaggtga ctgcttcgct ggatccagaa atggtgcgtg
     2101 aggtgctgga acttatcaat gatttggccc aagaaggccg taccatgatt ttagtaaccc
     2161 acgaaatgca gtttgcccaa gccattgctg accggattat cttcctcgac caagggaaaa
     2221 tcgctgaaga aggaacagct caagccttct ttaccaatcc gcaaaccaaa cgagcccagg
     2281 aatttttaaa cgtctttgac tttagccaat tcggctcata tctataaagg agattcttat
     2341 gaaactattc aaaccactct taactgtttt agcacttgcc tttgccctta tctttatcac
     2401 tgcttgtagc tcaggtggaa acgctggttc atcctctgga aaaacaactg ccaaagctcg
     2461 cactatcgat gaaatcaaaa aaaaagcggt gaactgcgaa tcgccgtgtt tggagataaa
     2521 aaaccgtttg gctacgttga caatgatggt tcttaccaag gctacgatat tgaactaggg
     2581 aaccaactag ctcaagacct tggtgtcaag gttaaataca tttcagtcga tgctgccaac
     2641 cgtgcggaat acttgatttc aaacaaggta gatattactc ttgctaactt tacagtaact
     2701 gacgaacgta agaaacaagt tgattttgcc cttccatata tgaaagtttc tctgggtgtc
     2761 gtatcaccta agactggtct cattacagac gtcaaacaac ttgaaggtaa aaccttaatt
     2821 gtcacaaaag gaacgactgc tgagacttat tttgaaaaga atcatccaga aatcaaactc
     2881 caaaaatacg accaatacag tgactcttac caagctcttc ttgacggacg tggagatgcc
     2941 ttttcaactg acaatacgga agttctagct tgggcgcttg aaaataaagg atttgaagta
     3001 ggaattactt ccctcggtga tcccgatacc attgcggcag cagttcaaaa aggcaaccaa
     3061 gaattgctag acttcatcaa taaagatatt gaaaaattag caaggaaaac ttcttccaca
     3121 aggcctatga aaaaacactt cacccaacct acggtgacgc tgctaaagca gatgacctgg
     3181 ttgttgaagg tggtcattaa ctcttaaaag ggactggatt ttaagctcca atcccttttt
     3241 aagattttac ctataacatc ctgagtctat ctaagatgtt caatctgaac acagtgtaca
     3301 tactttatct tctattgcat atactttatc acataagata cgaatatcct cttcactatg
     3361 actagcaatc aaaattgttg tccctttttc actagagagc tttctaaaca atgttctcat
     3421 attttctaca cttgatttat ccaaggcatt cataggttca tctagtaaaa gaatagaggg
     3481 attctccatg attgcttgag caatccctag ctttttcctc atacctagcg aataagtttt
     3541 aactttctgg tctttttgct catatagacc aactattttc agtgtatcat tgatttcctg
     3601 attaccaact actcctcgta tgcttgccaa atattgtaaa ttcttaaagc cactataata
     3661 atttataaaa ccaggttctt caatcaaagc tcccaaatta gctggaattt ttctctcagg
     3721 aacaatattt tccccattga ttaacacttc tccataagac ggactatata aaccagctat
     3781 taatttaaac aatacacttt tccctgagcc attcgcacca gtaattccta taatttcccc
     3841 ctgtttacaa ctaaagttaa ggttttgaaa aacacatgtc ttttttaatt tcaactcaat
     3901 attttttaat ataattattt cattcattct ataaacctcc tcttttgacg agtgaaatag
     3961 aaaatgcttt gaaaaagaaa gactaaaaat agcaactgaa gaaacaaatc tccccctaca
     4021 tccccattcc ctcgattcaa aatataaaat agataattag ttcgatttcc tacaaataga
     4081 ccaccaaaca caatcatgag taaaaagaaa ctaacgcaag caaagttcat ttgtacattc
     4141 gctcctagtg cgtataacat aaagcaaaac aagactaaca cacaagcatt caaagaatca
     4201 actataaaca aaactcctat atcccagttt ggtaactgta acataaattg aatcactaga
     4261 acttttacca tgattaaaat gagtccataa agaaaacagg tccaaatctg atacctaatc
     4321 cagagcttaa tagaagaaaa tcgaatcact tccataaaga aatcggatgc tcttctacga
     4381 tatacaactg ctagaaaaaa tgtatgaata ataaaccaat gaagcaacca tatagataag
     4441 actaaaaaag agttttgact cagattaggc ccttttaaaa tttctataac aagtctatca
     4501 tttccttcta accaatacca taatcctaaa caaatcaata tccaaaaaag aacataaccc
     4561 caaaagtact tggtatagat tatcttattc aacctccaca tgtgactact taactttccc
     4621 atttccaact tttacactct cctccagttt ctatgaacag aaaatacagt caatggaatt
     4681 atgatagata gtaatgtaag ccaataaatc aatgcatacg gattggaatt taccatatac
     4741 tgaaaaattg gcaataatcc aattatccca ttgttcaact gataaatcca gaataaggat
     4801 tctacaaata aaaaagtaaa cagaaatact aagctcatgt ttgatttttt aaaccaagaa
     4861 aaactgctaa tagtaagtaa ggataaaaag aaatagtatg ctatataaga gaaaaaaaat
     4921 cctataaaga aactagcatt gtttgcaata cttataccat aaaattctct taaaaaatca
     4981 acctccttta tctccaaaga gaagctaaaa ccattactaa atgcaattag aaaaatcaat
     5041 aaaaataaag tcgccgtcca aatccccgta ctaagagctg ctaatttgaa actaaaactg
     5101 gtaaagtgct taattgattt cagacgaatg cgacactcca acctattaaa atagttattc
     5161 atcaaataaa aaaagaacaa tatatatgtg aacggaaagc aatatactcc agtcgtcata
     5221 tcttgaagta aaactaaaat ccattctaat acatttggat ggattgaata ttggcgacag
     5281 cgcaataaat atactgtact agataaaaca caggatagca gtaatataaa ataaaccaat
     5341 actgataaaa aatctttttt gtaaaattga acaaattgtt tcattataca tagtcctctg
     5401 aatgtagaaa aaatgtacca taaacaacca aacaactaac aaataaaata aaagcaagat
     5461 gcccactaac taaggaaaga ctgatatctt tctgatatcc caaagctaat gttgtcacag
     5521 gttctaagta agatagccct aaaatagccc aaaaaatacc accaaccatc atataggcaa
     5581 ctgggatgaa aatagctcct atttttttct tcactagcaa agcactagct agtccaaaaa
     5641 tagagaacac agcgccccaa actccatacc agagagtcgt cacaagacta tagagcaact
     5701 gattagaatc aaataattct tttaaggcac cactataatc tccaatataa atttcctgat
     5761 aaggagtcac taaaagatta attcctaata ataataaata ggggagaaaa aagactagaa
     5821 aagaagaaat aattgcagta ctacctacaa tagccagata cttcttttta gaaattcggc
     5881 acaattgtgc tgttagaaaa tgactctcag catcctctat tatctgacta gaatagggca
     5941 gtgtacagat aagtgcagct actaggctaa tcggtgaaaa tcctcgaata gaagaagcgg
     6001 caaaaaaaat cgataaacct tcaattttat aaataccatt gaaagcaagg aaatttccta
     6061 aactcatgca aagaagggtc aaaaataaag acatataaaa tcgaggtgat tgaacgactc
     6121 gtacaagatt acaaatgaaa aattccatcc ttactcctcc ttataataaa aatagggtgt
     6181 agcattcttt tttcatgcta cacccacaat caaccatctt taaggcttac tctgacaagt
     6241 aagttaataa gaatctggac tccaagaacc tgaagtatga attcttacat gatttccaaa
     6301 ttgtggcgcc atagctaatc tagtaccaga accaatataa ttgtcaccac ctccattata
     6361 gtacatgaca atcctagagc cagaccccaa tgaatatacc ggggtaatat ctgacccact
     6421 ataggcgcta cgaatagagg tatttaacct tttaccgcca ccagtgctgt cactattatt
     6481 aattccagca gaggcgtttt ctttctcaag ctcactagtt gtatccgaac cccaacgcgg
     6541 caacgttaaa tcataatttc caatctgtct agctaaaact gtagggagaa acaaactaga
     6601 tgcaattgta actacgcaaa aaaggaatat atttttcttc ttttcatgac atcgtcctct
     6661 taagaagtga taaacaagat agttgattga tcaatttgat acatattaca ttttcattat
     6721 atcaataaaa gaaagcgttg tcaattattt tatttaaaat attgtcatat tttaaataaa
     6781 atggtaaaag agactcggta gcctcgtctc tctttatctt actgacctaa ccttcttgcc
     6841 aattttcttg gtcttcttta aagcgtttta gaagatcaag gccctctggc gtgatgtaac
     6901 cttcttcttg ggctagatgg ataagctctc tatagtttga aagcgtcaca agtttaacac
     6961 cagcatctgc aaagttctta tctgcttttg gcaattggta gctgaaaatc gctacaactc
     7021 caagtacatc tgctccttct cgcttggctg ctgctacagc ttcaagaact gaaccacccg
     7081 ttgaaataag gtcttcaacc actaccattt tttgaccttg agctacgcga ccttcgattt
     7141 gattaccagc tccgtggtct tttggtttgc tacggatgta ggcaaaaggc aagtccatct
     7201 tatcagcaat aatggctccg tgtggaatcc ctgctgttgc agttcctgca atcacttcta
     7261 cttcaggaaa ggcttcttta atagcttcca caaaaccatt ttcaattagg gtacgagttt
     7321 ctggataggc tagtgtcaca cgattatcag tgtaaatcgg tgacttgata ccagatgccc
     7381 aagtgaaggg ttcctctggt ttgaggtaaa cggcttggat tttcaagagg tggctagcga
     7441 tatctttagc aagtgtcatg gtattctcct tttatttttc taatctagtt ctttaattcc
     7501 agttctgtgt ccattcatcc ttgatggcat gataagctgc aacaggatcc tcagcttggg
     7561 taatgggacg tcccactacg atatagtcac tgccgatttg ataggcatca gcaggtgtca
     7621 tgactcgttt ttgatctcca actgcaacac cagctggacg aatccctggt gtcagacaga
     7681 taaaatctgg attggtagcc tgcttgatga cttgtacttc ctgagccgag caaacaacac
     7741 catccaagcc agcttcagct gtcttcttgg catagtgaat cacagactct tgcagactgg
     7801 tttggatatt ttgaaactcc tgcatctgag cttctgacgt tgatgtgagc tgagttacag
     7861 cgatcaattt ggcttgactc ccaagacctt cacgcgccgc cttcatcatc tctacaccac
     7921 cagccgcatg gacattagtc atatcgacac caagctgaga caagaccttc atggctgact
     7981 tgactgtatt aggaatgtca tgaagtttga gatccaaaaa gacactatga cccaaacctt
     8041 ttaagtagga cacaatctca ggccccgctg cgtaataaag ctccatccct accttgagat
     8101 aaaggctttc ttctgctggg aaaagagcta aaaattcctt gaccgcctca aaactaggaa
     8161 aatcaagagc aatgattgga cgatgttctc gcataggttt ctccttttac ctttactagt
     8221 gagagagtct ggccaacaga agccacgaaa aaaggaacct gccaacagca aggttccacg
     8281 aaaaatgggt agtctccacc ctttcaccgt ctaaccttag ctgcctctct ggactgcttt
     8341 aaaggttttc tattattcta ttatttctac tagcacttgt caagtaaaaa cttagtcacg
     8401 tatgtgttga ctgctattga acacctcaca agactctttc caaaagaata gttaaagact
     8461 ttcacttagg acagagaaaa agagtttgct tgttaccctt taattgaaca aatgggaatt
     8521 ctgttttatt ttgcggatgc ctattcatct tggcaaaggg gaagtaatga gaatgcgaat
     8581 ggacttcttc gtgagtattt tcttaaaccg atttggcaac aattaccgaa aaaatcctta
     8641 atggtgcttt gctagcttat taatcaccgt ccccaaaaat acttggatta caagacaggt
     8701 tttgaagcac ttatagagga aatttaaaaa tgttgcattt tttattgcaa tttatcagtt
     8761 atcttatcac ccaaaacttc tgcaattagt atgagcacta ttgttatttt tcttttttcg
     8821 tacactcaaa tcaaaatata gcaataagac tattctgtag aattgctgtc aaaaagatca
     8881 gttcattttt ccttcccact tgtgtttata atcattaaaa taactcaatt aagaaatttt
     8941 ctttttccta atagttatcg taattaaagt taatagaaga acaatccaaa taagtagtgt
     9001 tagattagga attagagttt tattccatga aaatccctcc cattttgtga aaaacttagt
     9061 aaacagtccc ataaaactat aatctgttat cttcgcaact gtcttatcca aaccagatag
     9121 catcggtaag aaagcaacta aaatcatagc aggaagactg ataacctgag ctactacttg
     9181 gctcttcgcg gttaaaccta tcaataaata gaataaaatg atggataaag aggttagaag
     9241 aacgattgta atataattaa aagtatgtac aattgtaact cctaaaataa gaggagtagt
     9301 tcccataatc acaaaagtta gcaaaaaagg aagaaacatc gttgataaaa tgtattcgga
     9361 tcctttaaca ccactcaaca gaagagtttg taaattgtac ttttcttttt cttcagacaa
     9421 gataatagtt ataggacttc caacagccaa agccaaagaa aaaggtaaac acatcatcaa
     9481 aagaactaat gcctgttgat cgttgacctt cccctgtgtt tccataagat atttataaaa
     9541 atatgtgaaa gcaaaaggca ctaaaacttg caataaaata ctcttattgg aaaggataat
     9601 ttgacaacgc aaccatacca aagccagaaa ccgtttaagc atttagcttt cctcctgtta
     9661 atgtgataaa aatatcttct aaagtaggct cacatgaatg aattgaaaaa atattttcca
     9721 gatcagcctg agatacctgt tctagtgatg taaaatcaaa agttatctga ttcccattat
     9781 aatctgtaac cttaatcttt ttatctttat tatatctttg aatgagttca gaaggagctc
     9841 cttgctcaac taatttccct ttatttaata aggcaacata atcacataaa agagttgctt
     9901 cattcatatc atgagtcgtt agaaaaatcg ttgtccctgc tgttttcaat tctaaaatta
     9961 actcatgaat tgttcgagaa gttgtgggat ctagacctga ggtcggttca tccagaaaga
    10021 gtacagcggg tttgttgata agagctcgtg ctaaaagcat tcgttgcctc attccagtgg
    10081 ataattttcc tgctaccatc ttgcgactat catataatcc tactcgcttt aacaaattat
    10141 caacacgtga tttactaata ttataaaatt tactataaaa aagaagattg ttatacagag
    10201 acattttctc ataaaatcca cttgtatcgc taaccaatcc aattctcttt aattcaccgc
    10261 ttgttaaatt ttgagatttt tgtcccaaaa taatagattg tcctttatcg gcaaggaact
    10321 gcccagtcag aatattaatc gttgtggtct ttccagaacc agatggtcct aaaaatctaa
    10381 ccttctttaa cagtgaagct tatattattt aaagctattt tatcaccaaa actttttgat
    10441 aaatggctaa cttcaatcat actcattgca cctcctttat attctacatt tttacttaac
    10501 atctatttac ttatctttat caaataaacg tcttaaaaaa aacaataaca ttaagcatat
    10561 aaccgttagc aagattgttt caatattatt gataatacga tacattctca agcagtataa
    10621 aataattata tatatgcaaa tgctcattat acctattttt ttaaacatac taagcctcct
    10681 tgctaaataa taaagttata taataactaa gacacactat acctaacata ataaatatcc
    10741 actggtaact gaataaatgt tctaaaatta acaagcaaaa ataaaatctc aaacgatgaa
    10801 aaaagccata ctatattttc caatgatgtt tgaatatttt tattgtagta agcataatat
    10861 atagcaaaaa taatacccaa gaacaatact attaattgtg tttctataac agtgaactgc
    10921 ccaatagcag ataaaaaagc tactcctcct gataataagc aaaaacaaac tgaacgaata
    10981 aatgttttcg ctctaatatt gttatctttc actttattat ttttttacaa attggacatt
    11041 ctccgttttc cagaacatat ttttcttgac ttagagcatg atttctcata ttataaacgt
    11101 aagcgttatc tattaggatt tcattataac atcccagaaa tattttaatt attaaattag
    11161 tcattatccc tcccaaaata gcaggcatct ctggtgtagt cggccacttt gtcttatcag
    11221 ataaagtgta cttatttata tctttgttta tattccgaca accaaagcaa gaaccgatgg
    11281 tagggatata tattatcagt aataagatag catccattaa accctcctga tatgtaggat
    11341 atcttatgcg atacagcaaa ttgattgaca atttttataa tatcaatggg cgtatcaatt
    11401 gctttaacga taaaatctat actcccatat tccgcaacaa ttttttctaa ttcttctact
    11461 gaagaaactt taatagaaat aggtactacc tgaacatctg aattatactt gtgtattttt
    11521 tcagaaagaa cattaatctt ctccttacca acatccgact ctgtataaag aatttgccta
    11581 tttaaatttg atggttctat tatatcgtaa tcagcgacaa taagtttcct cactccatac
    11641 atacttagac ttagagatat ataacatcct aaggctcctg ctcctaatac taatactgtt
    11701 ttattctgta ttttattata ctcttcatta taaaataacg aatagtaacc tttagttcta
    11761 gaatatctac tatcaatatc tatctcatcc gaaattaaaa tttgttcatt taatagttca
    11821 aatgttagtt tactgcctaa tacttcttct atctctaata gttccttaca actattaaat
    11881 agatcgttga taaattcttg cgaaatttga ggttctatag ctatttcccg catactccga
    11941 ccacgcaaat acattatacc ttctttttta taagcaaaaa cataactatt tacgtagaac
    12001 atatttcgtc cttcctctca tatataatat agaaagcagt atagaaacaa ccacccctcc
    12061 tatactacga acaataatat taggtgtcaa aaactgtaat agttgactca aagggaaata
    12121 aataataaca aatgctatta aaagttgaac tatatattga aaattcaagt tattattgtt
    12181 agaattcaag tatcttaaaa taataccact atcatactga aaaaaaggaa ttgaattaat
    12241 aaaaatcata cctaagttaa tgataaaaaa taagagtcca agcaatttat acgatgtgaa
    12301 caataaaaca agtaaacctg taagtgataa aatcgaattt gaagcaattc ctgctaacat
    12361 aattttcaaa aattgttttt tattttttaa gaattgcacc ccacttaaat ctacaaaaaa
    12421 aagccaaatt aaacaaaagt agcattattc cacaatttgg gatctcaatt tgataattat
    12481 tagcaattat tgcatgacta aattcatgta tcgttgcaga cataataaca acaagcaacc
    12541 aaaataaaag aatgtttagc gttattggaa agttttggaa attagtcaaa atatccgccc
    12601 tataaatata cataataaaa cattgcaaaa taagagccaa tgggaaaata ccaaatagga
    12661 ttttttcaaa attcttatta aatcttttat agtttaattt ttcagataat gttatattaa
    12721 agtcccaaga tattttaaaa atatttatct tttttcgtga tttcatagaa tctataagtc
    12781 ccattttact aagaacaata taaaatctta aataatattt cttccctact aacactaata
    12841 agtccgatat ggtaaattct tgacctatta gttgctctaa ccattttttt cctgtttccc
    12901 aactaataca tgtttcttac tagatggtat atatagatga tactgtccct cataaggagt
    12961 aggtaatatc ttaatcttat caacaattct cataacaaac ctaaccttta ataaataaaa
    13021 gaggggctaa gtaaaacata ctctagtccc tcagtccgca atcaataaaa taatgactaa
    13081 ttctattaat ctcttgctgc aagactaata cggaaaattc aaatgactgt ctaaaatgaa
    13141 attgaaaaag cttgatgtta gtaaatgaac aaacttcaac aaccagcaag agcaaggatt
    13201 gctaaaccaa atccgatacc tgttgcaagt ccagctgaaa aatcactaac tggtactgaa
    13261 atttcttcaa tttcctcaaa taaaaagtta tcttgcatga ttatcactcc ctatcttttt
    13321 aacaacaggc tattccaatt aagagaacta ccccagttcc aagtaaaact cccccaaaaa
    13381 ttcaccatct gtaggggcta cgatttcttc aatttcttca aataagaatg cttcatctga
    13441 catatgtgtt accttcttca taaaaaatta ttttgtaatc gattacattg caaatgtaac
    13501 ataaagaaaa acagatgtca aatattaaac gtaagaacat ggtcactaaa gaactataag
    13561 agaaaaggta aacctagcga cgcgatgagc gctgggtcgt ttgatttcga ttgctctctt
    13621 cctcttgttt tttctgttct tcttcctgtt ttttctcggc ttccttggcc tcttgtttgg
    13681 ctttttcctc agcctccata attaatttat ccgccacagt gtaactgtaa attccagctt
    13741 ccatgtcgac cacactcggt tctgacaatt gtggcttaat cttactgtaa tatggcagtt
    13801 tcttactcat ttcagataga ggaaccaaga cttcgtccga atcattcatg gtcaatcgaa
    13861 ttaaatcgga tgtcaccttg cttggggcta attccacctt ttggatagcc gccttgagtt
    13921 ctgggctaat ttgagcaagt tctgagacaa aaaccttgat ttgttcacta tcattaaaga
    13981 gaactgataa ataagtttct ggtaaactgt tcagactcac agaactagtc tcaagctgac
    14041 cactggaaag aataggataa tgattttcac cagaaatata gtaggccaca atatcatatt
    14101 ccttgacctt aatagtgaac ttagttggaa attgatagac aagttgagct gattcaaccc
    14161 aatagttaga cttaatctgc ttttcatatt ttgccttgtc tagcagaagg ttaatcgtat
    14221 aatccgaatc ctgaatgcct gaagcctgtc gaatatcatc agctgtagtt tgcaccgttc
    14281 cctcaacacg aatatctttc atggtcgcat aaggactgag caagtaggca gagacaatca
    14341 ataaaagcag acttggaaat aaaatcgtga aggctcgcaa gatatggata ccaggaatct
    14401 ttgctttggc tggtttttcc tttgtagcct ttttagaaag ctttttatcc tgttcctcct
    14461 tctctttaga ctctggttct tctttctctt ctttctcttt gtcagcctct gaggatgcta
    14521 ctttttcttc agactcttcc ttagctgatt ctgaatcttc ctggtctgtt tcactctcct
    14581 ggtcctgttt gtcctctgac ttctcagatt cttctcccat tcgagcttgt ctttcctttt
    14641 ccttctcctc agctagagcc gcctcttctt cagccttctt ttttagatat tcttggtttc
    14701 gtttctgcca ttctgataac tctttcaatt cttcgaggat ttctttgtcc tcatttttct
    14761 tatcttttga catttacttt ccttatgata aatctttttt caacaattga taaaaatctg
    14821 ctagagattt caattcctta gaagccttca tcttagcttg gtaatcttcc ttgtgactta
    14881 gtaagtgaga aagcttctct tccaaactat ccaaggtcaa atcgctttct tgaaggtctt
    14941 ctgcatagcc tttttttaca aagtaagctg cattttcaag ctggtcacca cgactagctt
    15001 cacgaccaag cggcacaatg acatgcaatt ttgctatcgc caagagctca aaaatcgtat
    15061 tggcaccacc tcgtgtcaca acaatatcag ccaattccat caagggttga tagagatcgg
    15121 tcacatagtc aacacgaaaa agattttggc tcaactcatt caggctagaa tctccagtta
    15181 gattgataat attgtagcgc tctgttagtt ctttcttatg gtctgtcacc aattggttaa
    15241 agacacgagc acctgcagaa ccgccaacaa acaatacagt cggcaattta tgattaaagt
    15301 ggctttgaat atccaccaat tcatctggtt ctggattttt ttgatctgaa accttggcca
    15361 ctgctcccac atgctcaacc ttagacaaac tcgaagcttg ctcaaaggtt gaatacatct
    15421 tagtcgcaaa tttataggcg attttattgg ccaagcccat agacaggtca gattcgtgaa
    15481 taaagacagg cactcctgac acacgcgcag cgataacagg cggtactgag acaaagcccc
    15541 cctttgaaaa aagggtctgt ggacgaagtc gcaacatgat aaagagcgat tggacgattc
    15601 cccagcaaac tttgaagacg tccagcatat tttgccaaga gaaatagcga cgcaattttc
    15661 cagtcgcaat agaatggaag gtgacatcca aacctgactt aaggatttct tggtgttcga
    15721 taccacgctt gtccccgata tagtggactt cccaaccatc ttcgatgaac ttgggcatta
    15781 acaaaagatt gagggtaacg tgtccaaccg tccccccacc tgtaaagaca atttttttca
    15841 tattattctt ttaactccgc tactgtgtcg ataaagaggt cgccacgtac ttcaaagtta
    15901 gcatacatat cccagctggc attggcagga ctaagaagaa ccacatctcc ttgagtcgca
    15961 agctcatagg ccttgcgggt cgcatctgca atatctgtcg cctccacata agcgacacca
    16021 gccttgtctg ctgcccgttt gacacgttct gcagattgac ccaggatgac catcttcttg
    16081 agtccagtaa tgtctggcac caattcgtca aactcattgc cacggtccaa accacctgca
    16141 atcaagacga ccttgctgtt atcaaatcct gacaaggctt tttgagtagc caagatatta
    16201 gttgatttac tgtcgttata gaatttaaca cccttgatgt catccacaaa ctggagacgg
    16261 tgtttgacac caccgaaggc tgaaagagtt tccttgatgg tttgattgtc cacaccacga
    16321 agcttggcta cagcaatagt cgcaagggca ttttccacat tgtggctacc tggaacaccg
    16381 atttcattcg ctgccatgac tacttcacca cggaagtaga gttgaccatc ttccagataa
    16441 gctccatcaa ccttttcaag tgttgaaaat ggtacaacag tggcttctgt cttggaagtc
    16501 aagtcttttg ccaagtcttg attaaagttc aagacaagga aatcagctgc tgtcatcttg
    16561 ttctggatat tccacttggc tgctacatat tccgaaaatg acccatggta gtcgatatga
    16621 gttggcatga ggttggtaat aaccgcaatc tctggatgga attcttgaac acccatgagt
    16681 tggaaagaag aaagttccat aacaagcgtg tccttatctg atgctatttg agcaacctga
    16741 ctagctggat agccgatatt ccctgataaa agaccatgtt gcccagcagc agtcaaaact
    16801 tccccaatca tagtcgttgt ggttgtctta ccgttcgatc ctgtgatacc aataatcggt
    16861 gcttctgaaa tcaaataagc caattccacc tcagtcaaga ctggaattcc cttggccaaa
    16921 gccttttcaa tcatgggatt gttgtagggg atacctggat ttttcaccat aagggcaaac
    16981 tcttcatcca agagttccaa aggatggcca cctgtaatga ccttgatccc ttcttccagc
    17041 aaactttggg cagctggatt gtcctcgaaa ggtttcccat catttactgt cacaatggca
    17101 cctagcttgt ccaacaaacg agctgcagat tcaccagact tggccaaacc taaaacaagg
    17161 actttcttat ttttaaattg atctattact ttcatgtctc gaactccatt tctactccta
    17221 ctattttacc atttttatgg aaataaaaaa gccacaaagt gtgtttgtga ctctttcttc
    17281 taactgaatc ttaccatatc atctatgtga taaatcggta actcgaatga cctgatccac
    17341 ttgttcccaa ataagaggat tgtgggtcgc aataataatg gtccgattcg gattttttaa
    17401 agattctagg atggaaagta attcctcaga gtttttgggg tctaaggaag tggttggttc
    17461 atctgcgagg atcaaaggtg gatcctttaa aattatcttc gctaatgcaa cacgttgtgc
    17521 ttctcctcct gataactcaa atataggttg cttcaaatcc aaataagaga ggtttacaca
    17581 gtttagagct tgtttcatca aagagatttt ctctttttcc ttcaactttt taccaactaa
    17641 acccagattg agattctctt tgacggtttg gctttcaatt aagccaaaat cttgaaataa
    17701 gtatcccaag taatctctaa agaaaacaga aggcttgatg tccttaagag aagtaccatc
    17761 atagatgatt tgtcctttgt catatggctc taatcgtcca atcatattca agaatgttgt
    17821 cttaccacag ccacttgtac cgattaaggc ataaattttc ccaccttcaa aatgaagatt
    17881 tgtatctgaa aatagctgac ggcttccaaa ttttttagat atattcttta gttcaatcat
    17941 cctattttcc tttcataatt gtcatagaaa cacgagattc tttctgcgct tgacggtaaa
    18001 gcgtcagaac tgcactagct agaaagacta ataaagtgag caagccaatc accaagtctc
    18061 gactgcttaa aataaagaga ctagcaccaa atacaaaact ggcaaattga ctaaccatat
    18121 actgagcatg tgtttcaaaa aatcgtaaac ctgaaattcg tttaatcaaa atatctcggc
    18181 ggaattgctc gaaatataga agattgacag aataaaatag taataaggaa ctagctatcc
    18241 ccacaatagc tcctaagatt aaagttgctg tttcagtttg aacttcatta taacgagtta
    18301 gataaacact tcttccttct ttaagatagg atacttgctc ataaattcca gctttcttca
    18361 agagttctag cccactctca tatcctttga taaagagttg ttttccagca ttgatagacc
    18421 aactagataa ggatataaaa ctatcacctg tagaagtcgg cgtgaatacc actaaaatcg
    18481 gatcagtcaa atactgagta gatacgggat tctcaccgtt attataaaca aaccgctttt
    18541 ctcccattga aagataacta acgtgcgctt tcatctcata atccaaagga gcacttgcct
    18601 cctcaccaga ttttccataa taactcaatc tttcttcaaa aactttctta agttctgctt
    18661 ctcgagagcg caaatgttct gggagcaaga ggataaactc acctttttgg agatgggcta
    18721 acttctgttt ggtctcagca tctaccacga ccttttcctt gtccaaataa ctgggactaa
    18781 catagagcgt attagcatct ggactatagg tatccagtgt ctctccctgt tcattttttc
    18841 cttgtggatt ggcaaaatgg agcagattat cctttacata aagagcttgt tcttcttcga
    18901 ttgcttcctt ggcaaaggca taccacttgc tctgattttc tgtatctttt cctctatcac
    18961 ctaagccaaa agaaatttga taatagtctg ctctatcctg ccatgcttgt tttgaaattt
    19021 caagttcttt caatcgttgg taagacatca aacctgtctt aacagcgtag cctactgtaa
    19081 agacagcaac caactgacac aatagggtta aaatcatcag gcgtttaagg ggtaatctcc
    19141 ccttaataac gggaactaat gctttgtaac tcaaactcat tagataaaga agcattagta
    19201 aaattgaaat cgccaataaa aacaacagat agaaactaat cccaaaacca taggtggcta
    19261 acaagatagg ataaaacaaa ccttgactaa aaagaacgac tcccccacct aggaaggaaa
    19321 ggagggctga tagaaggagc catttgatat cagtagataa agaatgcccc atgatggata
    19381 agagagtctg accagaaaag agttttatac ctgctgctct catttcctta atccgagtga
    19441 taatcactaa agcaaagaaa gataagccaa atattgctaa actaattaaa ataaggggat
    19501 ttagtaatat tcgaaaagca agaaaatagg gcggtatctt tcggtcagca cttgctttat
    19561 aacccaaatc tcctaattta tcggcaagct tttctttcgt caaggagcct gacaaaagga
    19621 gataactatt tagcggatca ctacgttcac gactttcttg gctagcttct tggaattctt
    19681 ttggtaaagt tccctgacca taagttgcat aagtaaagtg agtcgtccca tccttactcg
    19741 gctctacaat tcttctagct attaaactct gttctgagtt tgcaaaattc tccaattcct
    19801 gttcaaatac ctcacgcgtc ggttcctgag tatctttttt gacacgaagt aaagaaacgg
    19861 aatcatagct tgcatataaa tattgtggcg cacgtaagac aataatccaa gcaaggaaga
    19921 agctgagaaa aaaagttgat aataatatga atagtttctt catagtagac tccttgtaaa
    19981 caaaattccc cctgtaattt cttacaaggg gaacgattta aatcaatgaa cgattagtca
    20041 taatcacagt aaaatgctac ttgttctccc catttagtcc aaatccatgc ataggaggtt
    20101 tttccagcag aagcttctcc tttatctgaa tgactattcc atctacttac aagagaagaa
    20161 taatgatatt ttttaccatg atagtaattt gaaaaagccc aaccacctcc tgaaccttct
    20221 ccatacgtcc atactcctcc atctggatat tgtacagcag ctgatgcagc tcccaataat
    20281 gtaaaacttg aaataagagc tagagcaagt aatctatgtt ttttcgtttt cattttattt
    20341 tttctttcaa aaaaagcaca ccttgagcaa caatgcaaca aaataaaccc tcctctctct
    20401 tttgttgaaa ccgctttctt atgtgataaa aatatcattt ttattatttg ttgtcaagga
    20461 aaaaatcgaa ttttttagat attttactac attgcctcta tgaataatat tatatagtag
    20521 ttttatttta aaataatatg caaccagtac taaccaaata taaaatagat gccattaacg
    20581 aattttattc aagtttttcc cattcatact atccaagcaa aagagatgat gttatagtac
    20641 aaaacaattc aaactattgt aaaattccta gcaaaaaaga gagccaaaac tctctttttt
    20701 atcttctttt actttttttg actggcatga gtgtaatgtc tctaacacta aagtaagcta
    20761 ggatcaacat ggctattgct aggaatattt ctgttggtaa ttgaaaaatt ttcagaaaag
    20821 atagaaccaa taaaatcaag agtgccacta aaatacatac catagcgacg atattgactg
    20881 tccctttaat gctttctggt gtcgcaaata catagagtag gagcagtaaa attcctagga
    20941 ctaaatagac catctttctc tctttctagc tcttattcag ctgatttttt cttcttgtta
    21001 gctttctcac gctctgcttt gttaaggatt tgtttacgca aacggataga ctcaggcgtt
    21061 acttccatgt actcatcgtc gttcaagaac tcaagagact cttcaagtgt caagatacga
    21121 ggcgtcttga taacagctgt ttggtcctta gtagctgaac gaacgttggt catttgtttt
    21181 gccttcgtga tgttaactgt caagtcattt tcacgagagt tttcaccgat gatcattcct
    21241 tcataaacct cagtacctgg gttgacaaag atcgtaccac gttcttcgat agacatgatt
    21301 gagtaagttg tagccttacc agcatcgata gaaacaaggg caccacggtg acgtccacca
    21361 atttcccctg gaatcaatgg caagtattgg tcgaaggtat ggttcatgat accgtaacca
    21421 cgagtcattg acaagaactc agttgagtat ccaatcaaac cacgcgctgg aacaaggaag
    21481 accaaacgag tttgaccatt accagttgaa atcatatcca acatttcacc tttacgttca
    21541 gaaaggcttt ggataacaga cccttggtat tcttctggag tgtcgatttg tacacgttca
    21601 aatggttcac atttaacacc gtcgatttct tttacgataa cttctggacg agatacttga
    21661 agttcatagc cctcacgacg cattgtttcg ataaggattg acaagtgcaa ttctccacgt
    21721 cctgaaacag tccatttatc tggtgaatca gttgggtcaa cacgaaggga aacgtctgtt
    21781 tgcaattctg cctgcaagcg ttcttccacc ttacgagaag ttacccattt accttcttta
    21841 ccagcaaatg gtgagttgtt gaccaagaaa gtcatttgaa gagttggctc atcgatgtgt
    21901 aggattggaa gagcttctac tgcatctgtc ggagtgatgg tttcaccgac aaagatgtct
    21961 tccatacctg aaacggcaat caagtcaccc gctttggctt cttggatttc acgacgttcc
    22021 aaaccaaaga aaccgaagag ttttgtaaca cggaagtttt tagttgtacc gtcaagttta
    22081 gaaagggtaa cttggtcccc aaccttaact gtaccacgga agacacgacc gataccgata
    22141 cgtccaacga agtcattgta gtccaaaagt gacacttgga actgcaaagg ctcatctgag
    22201 ttatctactg gagctgggat atggtcgata atcgtgtcaa agattggtgc catagtcgct
    22261 tcttggtcag ctggatcatc tgacaatgaa gaagttccgt tgatcgctga agcataaacc
    22321 actgggaaat caagctggtc gtcatctgca ccaagctcga tgaaaagttc caagacttca
    22381 tccactactt ctgctggacg agctgatggc ttatcgattt tgttaacaac cacgattggg
    22441 acaaggtctt gttccaaggc ttttttcaat acgaaacgag tttgtggcat ggttccttca
    22501 taggcatcta cgaccaagac aacaccgtca accattttca tgatacgctc aacttctcca
    22561 ccaaagtccg cgtgtcctgg tgtgtccata atgttgatac gagttccgtt gtaagcaacg
    22621 gcagtatttt tagcaaggat ggtaatccca cgctcttttt cgatatcgtt tgagtccata
    22681 gcacgctctg ccaattcagt acgtgcatca agcgtttctg attgtttcaa taattcgtca
    22741 accagggttg ttttaccgtg gtcaacgtgg gcgataatcg caatgttacg gatatcttct
    22801 cttaattttg tcatgatttc ctctataata ttcaaaattt attttctaac tgaacgatta
    22861 taccataatt tcaaataaat aacataactc aagcaagtgt aaatgttttc actccgcttt
    22921 tcttttcacg tcaagccttt tcaaagcaag cgacttatga taagataggc acagtatgcg
    22981 tttagataat ttattagccc aagaaaaagt tagccgaaag gccatgaagc aagcactcct
    23041 cagaggggaa attctagtcg atggttgccc agcccgatcc ctagctcaaa atatcgatac
    23101 aggactacaa gaactccttt ttcaggatcg aatcattcaa ggctatgaac acacctatct
    23161 tatgcttcat aagcctgctg gtgtcgttac agccaacaaa gacaaggaac ttccgaccgt
    23221 catggacctg cttccatctg acatccagtc tgacaagctc tatgccgttg gccgactgga
    23281 ccgagataca acgggactcc tcctcttgac cgataacggt cccttgggct ttcagctcct
    23341 ccatccccaa tatcatgtcg ataagactta ccaagtccag gttaatgaac ttctaacacc
    23401 tgaccatatc caagcctttc aaaagggaat tgtcttttta gatggtactg tctgtaaacc
    23461 cgcaaaacta gagattctat ctgcaagtcc ctccctcagt caagcctcta tcaccatttc
    23521 agaaggaaaa tttcatcaaa tcaagaaaat gttcctctcg gttggtgtta aggtgactag
    23581 cctcaaaaga atccaatttg gggacttcac attgaaccca gatttagcag aaagtaacta
    23641 ccgccctttg aaccaaaaag agttacaaat cattaaaaac tatttagaga tgagtcgata
    23701 aaacaaaaaa agctttaaaa ctaaagcttt tttcttttat ttaccgaaaa attaaggcga
    23761 ttgctacaat ccagttaact acagaaatca caattcctaa gatattaaga atcttttcta
    23821 ttttatagtc taattgtgac tctttttggt atgaaatagc caagaccaat cctatgatac
    23881 ccaaaatcaa gcctacaatt ggaaataaca aaccaagaat aatcgacaag atacccacaa
    23941 aaagtggatt tttcttcttt tctttcatgt tctaagaact ctttaaattt tatacaaatt
    24001 aattatacta taaaacaata gcttcatcct atcattcgac taatttggaa ataaggttag
    24061 ctagtcttca ctttcccttt ccaagaatcc aagccataag aaaggatata aatctcagaa
    24121 aaaccttgtt ttttcaagta aagagctgca tttgtaactc gttgcgcacg ttggttctcg
    24181 tagagaagga caggtttatc tttacgaagg gctgcaagac tagttttcaa ctgacttgaa
    24241 ggaatattgc gtgcaccaag gatatgtttt ctgtggaatt ctgctgggtc gcgcaaatca
    24301 atcaattgac ccgtacgaat caaggcttca aactcctcat tgtccacaat tttagccgca
    24361 cgacgaatac gaagatagtt aaagcccatc cacgccaaca ttgctagtat aagtgcccac
    24421 aaaatccaag taaccattag ttcttttctc catttttctc aatataatcc aattctacct
    24481 tgtgctctct gcgaagaact gcttctgcct ctagatagtc taatttatcc atcaaccctg
    24541 catcgtaaat ccgagatagt tccaacttca tcagttcaat atcatataag cgttttccca
    24601 tgtaaacaat aataccaaat cgtttgagga attgctgcac atcatagaat gttttcataa
    24661 gactcattct agcaaaattt tgtgtttttt tcaagaagag actcacacaa tgctccttat
    24721 tttcctatct tctttggcga ttctaaggca agtgtggtac aataaaaaca tgagaattca
    24781 acaattacat tatattatca aaatcgtcga aactggctct atgaatgagg cagccaagca
    24841 actctttatc actcagccaa gtctttccaa tgctgtgaga gatttggaaa atgaaatggg
    24901 cattgagatc tttatccgca atcccaaggg aatcaccttg acccgtgatg ggatggagtt
    24961 tctctcttat gcccgtcagg ttgtcgagca aactcagctt ctggaggaac gctataaaaa
    25021 tcctgtcgcc caccgcgaac tctttagcgt ttcgtctcaa cactatgcct ttgtggtcaa
    25081 tgcctttgtc tctttgctca agaaaagcga tatggagaaa tacgagcttt tccttcgtga
    25141 aactcggact tgggagatta tcgacgacgt caagaacttc cgcagtgagg tcggggtcct
    25201 cttcttaaac agttacaacc gtgatgtttt aaccaagatg ctggatgaca atcacctact
    25261 agcccaccat ctcttcacag cgcaaccgca tatctttgtc agcaagacca accctctggc
    25321 aaagaaagac aaggtgaaac tgtctgattt ggagaatttc ccttacctca gctatgacca
    25381 agggacgcac aactccttct acttttcaga agagattctt tctcaagaac accacaagaa
    25441 atccattgtg gtcagtgacc gtgccaccct ctttaatctc ttgattggtt tggatggtta
    25501 taccattgcg acagggattt tgaacagcaa cctaaacgga gacaatatcg tttctatccc
    25561 actggatatt gatgacccga ttgagctggt ctatatccag catgagaaaa ccagcctatc
    25621 taagatgggc gaacgcttta tcgactatct actagaagaa gttcagtttg atagttgaga
    25681 aatgataaga accaatatgt aggctagcaa caacctgcac attggttctt tttacttata
    25741 attaaaagtt tcccctgcca acttatcagc tagcttggga aagagagtat aaaacttatg
    25801 ggctaggttc aacaaaatcg ggagattgag ttctcgttta ttttttccta taatcttgac
    25861 aatcttttta gccactgcat ctgcctctaa gaggaagcgg tcaaccgatt taagataagt
    25921 tccatctggg tcagcttggt caaaaaatcc tgttcggatt ggacctggat tgactgttgt
    25981 cacatagact ccatagggca taagttcgag tcgcagagca tttgaaaaac caatagccgc
    26041 aaacttggtc gctgagtaaa gactagactt gccagtagct attagacctg ccatgctgac
    26101 gatgttgatg atatgccctt tgctgctttc cttcatacga gccgcaaggt gacgagacag
    26161 attcatcagg gcaaaggtat tgacctcaaa catctggtga atatctttat cagcaatctg
    26221 gtcaaatccc tcaaaaatcc cgtaaccagc gttgttaatc aagacatcaa tcttgccata
    26281 gcggagataa agatcagtta ccagagcttc tagggctgaa tcgtcggtaa tatcaatttc
    26341 aatcaattct gcatgggaat aatttccgta gagttgggct aatttttcct tatttctacc
    26401 aagcaagatg agttggtcat tgggcaggag tttgaccatt tcttgagcta gaccaccgct
    26461 agctccggta atgagaatag taggcatact tatcctttct gtgactgcta gatttccact
    26521 tcttccaagt ctttgaccac atggacattt tcaaaaattg tggcagcgtc cttcttgagt
    26581 ttgcttatat cttttgagag gaaacgggca ctgatatggt tgagtaggag gcgtttggca
    26641 cctgcttcta ccgctacttg tgcagcttgc atattagttg agtgaccatg gttacgagca
    26701 attttttcat cacccttgcc ataagtggac tcatgaacta ggacatctgc attgacagcc
    26761 agacgcacac tggcatccgt ttttcgagtg tctcctaaaa tagtgataat cttacctgga
    26821 cgtggcgctg agatatagtc tgctgccttg atttcagttc cgtcttccaa aacaagatcc
    26881 tggccgtttt tgattttacc aaaaagcggg ccgaacggaa caccagcagc cttgagtttt
    26941 tcagcatcca gcgtcccttc tagatccttt tgcatgacac gatagccaac acagaaaata
    27001 gtgtggtcca gctcctctgc atacacagtg aatttatcgg tttcaagaat tttacccaga
    27061 gaatcttggt caaactcatg gaaatgaatg cggtagggca gacgagaacc tgacacacga
    27121 aggctggtta agacaaatga cttgattcct tgaggtccgt agatttccaa atctgtctgc
    27181 tcttcattgg cctgaaaggc acggctagaa aggaaacctg gcaaaccaaa aatgtggtct
    27241 ccatgcagat gagtaataaa gattttgctg accttacgtg gtcgaattgt ggtttccaga
    27301 atgcgatttt gcgtaccttc tccacagtca aagagccaaa cttcgttaat ctcgtccaaa
    27361 agtttcaggg cgagacttga aacgttgcgg gctttagagg gctgaccagc ccccgttcct
    27421 aaaaattgaa tatccattcg atactttcta attaatcaat atataacatg gctgtgcggt
    27481 tttccgatcg gaaatagcgt ttgccagaaa aagcagcagc ttcttgcaat aaatcctctt
    27541 ggctgtagcc tttgagacgt tttcgaccat cagccaatct ttccaaatca gtcaaagctg
    27601 tgagactttc taggctgaca acttcctcgt ccttgacagg cttcatgtaa atcttaccag
    27661 actcttcaaa gactaattga tgggagaaaa tttgcgcaat ttcaaagagc aagtcatccg
    27721 agattttctc ctcattttca gagaaaatcc gaccaaagcc gtcactctca taacaaaaac
    27781 caaaggattt accagacaga ttaagccgaa taaaaggctt attttctagg gtgaaacttg
    27841 gctcagtatt gtaaagattc agttcctgac tgagttctgc aaaataatcc gtcgcagcct
    27901 gaggactttt tttctgatag agttctgcaa agtaggcatt aacaacactt ggcggaggtg
    27961 taataagtgt taactgctcc tgctctgttt taccagctag aagctgatcc agatagacct
    28021 tgtccagact tgtataacct ccatacttta gagccaaagt tttaatatca gtcataaaat
    28081 tcttctaacc tccatttatt tttctcggaa atgtagcctg taatcacttc gccgtcttcc
    28141 tgataatcac gttcttccag aattgcaaca ctctctaaat catgaatctt gtaggacttt
    28201 gaaaaaggca ctcgcagggt aaatgcttca aaaatttcct taatcttatc tagcaataat
    28261 gcttgcaagt tttcacgact gtcctcagac ttggcagaaa tgagggtata tggcgtttgg
    28321 gtaggcgtga aatcctccac caaatccgct ttattataaa gcgtcaagtg aggaatatct
    28381 tccatgtcca ggtctttcat gatggagaga accgtttttt catgctcctc gtggtaagga
    28441 ttgctagcat cgataacatg aaccagaagg tccacatgct tgctttcttc caaggttgac
    28501 ttgaaactgg acaccaactc tgtcggcaaa tcttggataa agccaacagt atctgtcaaa
    28561 gttacttgga gattgcctcc cagatgaata ctcttggttg tcgcatccag agtcgcaaag
    28621 agctcatctg cttcatactg ggtcttactg gtcaagatgt tcatgatagt tgatttccca
    28681 gcattagtat aaccaatcaa accaatctta aaagtgctag actccaaacg tttttctctg
    28741 acagtcgcac gatttttctc aaccacctta agctggcgct cgatatccgt gatttgattg
    28801 cgaacgctac gacggttcag ctccagttgg ctttcaccag gaccacggga accaattccc
    28861 cctgcctgac ggctgagcat aatcccctga ccaaccaagc gaggcaagag gtatttgagt
    28921 tgggctaggt ggacttggag cttcccttca tggcttcgag cccgcatggc aaagatatcc
    28981 aaaatcaact gcatacggtc aatgacctta acaccgagaa cttcctctag attgacattc
    29041 tgccttgggg tcagacggtt gttgacgatg acagtagtga tttcttctgc atccaccata
    29101 agcgcaatct cttccaactt accagagccg acgaaggtct tggaatcata tttttcacgt
    29161 ttttgtctgt agctatctac aacgactgcc cctgccgttt tcgctaaact agccaattct
    29221 tccatggaga ggtcaaaact gtccataccc tgcaattcca caccaatcag caggactcgc
    29281 tcctcttttt tctccgtttc aatcatctaa aaactcctct atctggctta aaatgcggtc
    29341 ttgtacacca gattctccaa tctgataaaa ggtgacctgc atgcgattac ggaaccaggt
    29401 cagctgacgc ttggcaaaac gacgggtcgc ctgtttaaga ctctcacgag cttcctccaa
    29461 ggtctgctct ccacggaaat aaggaaagag ttccttatag ccaattcctt tagcagcctg
    29521 tacattaggg gaatggtcaa acagccactt ggcctcatcc aaaagcccag cctcaaacat
    29581 caaatccact cggtggttga tacgctcata aagttgacta cgttcatcat ccaagcagat
    29641 aatcagcggt tcatacaaaa tctcttgatt ttccaaatcc tgaccaaaat gggcaatttc
    29701 taaggcacgc atagcacgac gacgattaaa ctggggaatc tcaaggcctg cttgctccac
    29761 caaatgggct aattcctcat ctgaatatgg ctccaaacta gctcgataag ctaaaatctc
    29821 ctcatgagga gtctccccac ctaggtggta accttctagc aagctctgga tataaagtcc
    29881 agtcccaccg gcgataatgg ctagcttgcc acggctgtga atatcctcaa tagtcatctt
    29941 agcttctgaa acaaaatcaa aagccgagta agactcagtt atctctctaa catcgattaa
    30001 atgatgagga acagctgcct gctcttctgg actagccttg gccgtcccaa tatcaagtcc
    30061 tcgatagact tgctggctat ctccactaac cacttcgcca ttaaagcact ttgcaacttc
    30121 aatagctaga gccgtctttc caacagcagt cggtccaaca atcacaatta tttttgtttt
    30181 catctttttt ccttgaaaaa ttcccatttt ttcgttacta ttattataac acaaaaaggt
    30241 cagtcagaaa aatgtgacct cttgaggagg ctggctgatt gagaatataa aggaggaaga
    30301 ctcatggcta aaggattcgc taaaggtctt gtaacaggtg tcgcaggaac tgtcgctgcc
    30361 gttgcaggtg cagtatacgc atttaaaaag aaagtaatcg aaccagaaga gcaaaaagca
    30421 gctttcatcg aagaaaaccg taaaaaagca gctcgtcgcc gcgtatcacg ttaagaaaca
    30481 aaagaagttg ggattattct caacttcttt tttctattct tttaaatagc taggtcaaac
    30541 ttcaactgtg gcttaacagg gtcataatcc accaactcaa aatcttctgc tttgatatca
    30601 aagaaattag tcccatcagg aacatttaaa accaagcgtg gttggcagtt tgacggctcc
    30661 cgacggagca attcctgagc ttgttcaaat tgattatcat agatatggag gttgttgatg
    30721 aagtagaaga acttcccaac cttccagcca aaatgtttag caatcatcat ctgcaaagcc
    30781 acatactgca tagcgttgat gtggtgggcc accagcatat cattggagcg ctgggtcaag
    30841 gtcgcatcca gatagatttc cccatcaaca cgccgaacat caaacatggt ctgaaaggcg
    30901 cacgggagca gcccatctgt ttcttcgaaa gcttggtaat cccagagaga aataatattg
    30961 cggcggttcc aaggattggt ttccaactgt ttgagaagct tattgataat gtcgtgtttc
    31021 ttaacaacgg caccatagcg ctcaccaatg gttcccgtgt ctccaacttc ccagtcattc
    31081 cagtagtgaa cattgtactt gtcattaagc acttctaagc tatttgactg gtcttggtaa
    31141 atccagagaa cttctttgat ggcggatttg attgcaatgg gacgcaaggt tatgatagga
    31201 aattctcctt tagataagtc gtactcggaa aaggcaccag ttacatactt ggaattagca
    31261 acagtcccat ccttgtactt aggacgagcc tgctctgaga aaacaccttc ctttagaatg
    31321 cgttcaatat tctctttaaa aatcgtatct gcttttgtca tttactctca cctcattcat
    31381 tgtcctaact agtatagcat aaaaaataaa agaggaacat tactaactct aggttagcat
    31441 tttcctctct cattatctta ttgcaataca agtgatgctg ctccgataac tccagcgtca
    31501 tttcctagag ttgcaagagc taatttagta gatgtgcgta cttgtgggaa agtattttcg
    31561 tcatagactt tttgaacacc ttgtagaagg aattctcccg cagctgacac accaccaccg
    31621 atgacgattg ttgatgggtt taggattgaa ccaatgttag cacaagcgat tcccaagtaa
    31681 cgtgagaagt tacggtaaac aatcaaagca aggtcgtctc cttcttttgc gagatcaaag
    31741 acagtcttag cagttacttc ttctccgtta tcaatcaagc gtttcaaggc tgcatcgcct
    31801 tcgtattcat cggcatagcg acgagtcaag ttgacaattc ctgttgcaga agcgactgtc
    31861 tcaagacagc ctttcttacc gcaagtacat gagattggct ggtcaaagtc aacagtgatg
    31921 tgacccaact cacctgctgc accagcaaca ccgtgaagca atttgccttc tgcgacgata
    31981 ccgccaccaa caccagtacc gagtgtcata aagacaacat ctggttggtt atcaccagca
    32041 cccatccagc gctcaccaag agctgctacg ttggcatcat tatcgatgaa aaatggaatg
    32101 cccaaagctt tttcaatctt ttgtttgatt ggttgaaggg ttttccagtt caagttgtag
    32161 gcaccgataa cagtcccttt gtcacggtca accacacctg gtgatcccat tccaatgcct
    32221 tggaagtccg ctgctgccaa tccaagcaag tccaaacgat gttgaataga ctcaatcata
    32281 tcatcaacga tatgacttcc ctcatccaaa atgttggtct tgattgacca ttttccttgg
    32341 atttctcctg ctgttgttaa gattgcaaat ttgatagaag ttccaccaag gtcaatccca
    32401 ataatctttt gactcattat attctccttt ttcattgtgt agtaattgaa aatgcttaca
    32461 gtactagtat atcaaaatcc attcacttat gcaaaggttt gcgtaaatga attataagga
    32521 attttttcgc ctaccagtat ctgttaacca tctaaatgat caatggcata ttgagctgca
    32581 tcatttgaaa atttatcaaa atcagaagtt aattgtctat acatacgttt ttttgacatg
    32641 tggaataacg aattataact ttttgccttt cctaaagcag cagaatattc actattgcta
    32701 tcattactag aagaagctgt acttgcttga ccttccttcc aaactccgtc agcgccaaca
    32761 taataacgtc caccatcaac ccattcattg acagccattt taccatccga ttttagatag
    32821 taatttcctt gccatgtact ataagcataa gaaccatctg atgtcaaata ataccaagct
    32881 tgataggtgg catcataaac ccattcacct ttagccattt taccatctga tttcaaatag
    32941 tagtttcctt gccatgcatt gcgagcatat gaaccatctg atttcaagta ataccatgct
    33001 tggtaagaag aatcataaac ccactcacct tgtgccattt taccgtttga tttaaggtaa
    33061 taagctcctt gccatgcatt ttttgcataa gaaccatctg atttcaaata ataccaagct
    33121 tgataagaag agtcataaat ccattcactc tgtgccattt tgccatcagc tttcaggtaa
    33181 tagtttccct gccatgcatt tttcactaga tttccatttc catcaaaata gtaccaagaa
    33241 ccgtcctgct taacccaacc acttgctgtg gatgaagttg tatcagtttg agttgtagcc
    33301 gcttgttttg ctttaaaagc acccttagga gcatttttca attcgcaagc cacaccatca
    33361 tgatcgcggt ctaacttggc agaataacca ggttctccct catgaagatc cgaatatcca
    33421 ttagcccaag cttccttaca gctcgaaaaa tggatgtttt cttccgccaa aactggttgt
    33481 gaaaacaagg ctaaaattgg caacgtcacc agactcattt ttaaaaatag acgtttattc
    33541 atttctttct cccataatta tgatatcgat ttcaatatta ttttatcaaa cttatttcag
    33601 caattcaagg aaacttctgg tcttttctac aaactccttt ggttttgcct tattcaagac
    33661 atgagggcca tcaggaatga tctgaaactg cgaatctgtt aacagtctat ggagagcttt
    33721 catagaacta agattcggtt tatctttgct gccacaaatt agtaaggttg gataggagca
    33781 agtccctgct atatccgtta aatcaagtgt cttcaattcc tcagaaactc cgaccataag
    33841 agtcttgtct gctccctgtt tttcaaatac tcttttggga agtagtttaa aaatcagcaa
    33901 ctgaagataa aataggatat tccctgctaa tttaagcggg catcctgaca gaatcaaagc
    33961 tcgaagattt ggtaaatcgt aactggaaag ttctagtgtc agggcagcac ctaaggacaa
    34021 tccaatcaaa acaaaaggtt ctgtctcttg agctaggtgc tgataaactc gctctttagc
    34081 ttgttgatag ttactaactc cagaaggaaa taactcgata gcctcagaag gataatctgt
    34141 cagtagattc cgaacttctt tccaagactc tgctgactgc cctaacccat gcaaaaatat
    34201 caatttcatc tagttctcct caaggcttag ttcatacaag cctctcactg cattacagcc
    34261 gtaaatagct tctgcttggg ttaaatctgc caaggtcaag actttctctt ctacctgtcc
    34321 tgtttctagc aaatactgac ggtaaattcc tggtaagatt ccaagtcgga taggcggtgt
    34381 gtagagtttt ccagcgattt tcagaaccaa atttcctata gaggtttcaa gcagttctcc
    34441 tgacttattg tggtaaatct tctcttgttc tcctaggctc aaatgcggtc ggtgagtggt
    34501 tttaaagtag gtaaaggatt gattcaaagc agcttcctga aggcagactt gggcctgaca
    34561 aaagcttgta ctgagagggg ttaatacttg acgattgact tctatctctc cagatttgct
    34621 aaggctgatt cgcaagcggt aatctcgatt agcttcacaa tcctgacact cttcctcaat
    34681 cttgtgtccc aagtcttctg catcaaaagg aaaagcaaaa taacgactag cttttctcag
    34741 tctttccaga tgttgatctt caaacagcag gttcttctgg ctgatttccc ctgtcgtaat
    34801 cagttggaag cgagcttgtt tacgataaag aactgctgcc ttttgatgaa cctctcggta
    34861 ttcagattcc catgtgctat cccaagtaat ccctccgcca actccataaa tggcttgacc
    34921 tttgtgaagt tgaatggtac gaatggccac attaaaaatc cgtcgtccat ttggaagcaa
    34981 gagaccaatc gttccacagt agactccacg cggttgaggc tccaagtcct tgataatctc
    35041 cattgtcgca attttcggtg cacccgttat ggaaccacaa ggaaagagtg agcggaagat
    35101 ttcaacaagg tccacatcct ctcgcaactg actcttgatg gtcgaagtca tctgccaaac
    35161 agttgaatac tgctctacct gacacagacg ctccacgtgc tcgctcccaa cttcagaaat
    35221 acggttcata tcattgcgca agaggtccac aatcatcata ttttcagagc gatttttggg
    35281 atcctgttcc aaccaactgg cctgttcaag atcttcttgg tcagttaccc cacgctgagt
    35341 cgtccccttc attggtcgtg ttgtcaactc gcgatcattt tgctcaaaaa agagctctgg
    35401 gctcatggaa atcactgcca tctcgtcatg ttccacatag gcattgtagc ccgcctcctg
    35461 ctctaccacc atacgattgt agatggcaaa aggattggca cttaactttt gcttgagttg
    35521 gacggtgtag ttgacctgat aggtgtctcc ctgcctcaag tgatggtgta tctgggcaat
    35581 ggccttttca tagtctgctg cagacgtcac ttcctgccaa tttgagggca aatcaatatc
    35641 ctcataagtc agaggaatag gggaagtttc tacgctatca tgaacagtaa agtaaagcag
    35701 gtactctccc agtaggggat ccttgtgaac tgctaatttt tcctcaaaag caggtgcagc
    35761 ctcgtagctg acatacccca ccacataata accttgctct tggtagcttt ccacttgtgc
    35821 cagcaaatct gccacttctt ctacatttct cgttttcaac tctttaatag gctgggcaaa
    35881 ggtatatctc tcccctaaag tcctaaaatc aatcactgtt tttctatgca taccttaagt
    35941 atagcataaa aaggcagata cagacaaagc tgatctacct ttttacgatt ttattcattc
    36001 tatgcttgtg gtgcagtaaa tagatcacca gagaaaccgt ctgtgttttt aaggagagct
    36061 ttaaagactt tcgatttcaa gacaactgta tttcggtatt tgtctttaat actactatcg
    36121 tttagttccg ttaaatcttt agtaacagcc tcatcaaaca tagtcttcaa ctgttcatag
    36181 gttgaaattt gagtgccatt aatttcaatt ggtgcaaaac cttttttaga ttttgtaaga
    36241 acttccccat accattgctt cttccaatct tcaaggtttg aaaattggtt attagaaact
    36301 ttcttaataa tgaaatcatc acctaataga ccgttatttt ctttattggc tgcaccttga
    36361 tgtttgcttg aaacatagtc aatgaaccca tgttcatatc catagtaacc ccacatacgg
    36421 aaggcgttat gtttgaagga tagagaacca ggggcacctt gactcgtatt accgccataa
    36481 ataccagcca tcatattaac attgacatat gccgtaccaa agtcctctgg gtggaagacc
    36541 ttattacctg gatttccgtg aatggtcatg aaattattat ttaccaagtc atcgatggtt
    36601 tcaaggttag ttgttttctc agcgtctatc aaatcacgca ctttgtccca ttggtgagga
    36661 ccacttagtt tatttctgtc cattggtcga cgtgtttcgc gatcaatttt cttgaaccac
    36721 ttactattgt cacctttaac cttaggaaga acagcctcag cttctgcata atcgagcatc
    36781 atcatagctt cattgtagtt cttcatgtaa tggtcgattt ggtcacgtgt ttgaagtttg
    36841 gtaggatctg gattatacca ttggtttcca tcatttgaac gttcaaatgc catatttaat
    36901 ccaagtgcgc catattcacc ttgatgtcca tgttctgctg gagtttgtaa cataccttga
    36961 gcaaatgctt caagatctgt accctgtcga tgtctatgac ctcccagata tgccattcgg
    37021 tcatttacgt gggtagtctc atgtgtaaag gcagatgtgc catattcact tattaaatcc
    37081 gacataacaa aatatacagc atccataggc tgtggatcag caaagatgta agcatacgct
    37141 ccatacccat acttataatc atgatactta tcaatacgtc caaagaattc acgaataggt
    37201 gcataatccg gtttatcttt tcgatgacca aatctatcag gccaaccacc gggtgcacca
    37261 tgattatccc atacaggagt tggaactaca ttttgactct taagaagttt atcgcggaca
    37321 ttatcagtgg caaggcgtga ccaaaaatct aagtaattaa cttgctcttt agcacgttta
    37381 ttgatttcac tcttgaacga ttcacgtttt tcttctgtat ctttaccata cttttcaaac
    37441 gaactaaatg agatagtatt atatgttgaa atcataaata aatgtgcatc tttcaagttc
    37501 agaagcgata aaatcatgcg tccatgttgt ccgttattaa gtccttcata tagacgatat
    37561 tttttattag caaatgcagg gttagttgac ggttgttcaa ctacataagc attattttca
    37621 atagctttct taaaccaaac atttatgtct gtttcatttg tgaatagttt catattgtag
    37681 gtcaagaagt catgtaagtt tccagaaaga gtatttttcg ccaccacttc acggaaggac
    37741 tcctgtgtac gacttccctt aatatagtcc tcttttgaac caatttctat taatcgatct
    37801 aaaacactta cattcttacc ataaaaatct ggtttgaata gcattaattc tttaatattg
    37861 aagttatcaa atttcacacc gtaataacga tttaggtatg ttaatcctaa caggacagca
    37921 gctttgttat cttcaatttt cttaatcaag gcacgttttg cagcttcatc tgtattcagt
    37981 tgatgatctt cattttcaac taattttgtc acaaatgtat taagatgatc ttttacttct
    38041 ttaaagcttt cttctaagta aagattctta attgcattaa ctctgttatc cccagttctt
    38101 cctaatcgtt gataaattgg atcagattgt aattcaattg gacttaaaat accttcaata
    38161 gcactaatta aatctgcacg atctttaaca accatatttg gtgtatagac gacttcacca
    38221 agctctgcga cactgtattc ttttacttgc ttaacttgag agtctttagg agaaatcgta
    38281 aagatatctt ttgtcttatc tgcgtaatga accataatat ggtcagcatc tgtaaggtct
    38341 gtaataaagt cattaccttt cattgcagtc acagacaata cttctttatt cattaatgga
    38401 ctatcagttg taagcttgtt cccttgattc acaatccatt ctttgttgta gaacggttgg
    38461 agtttttcaa cattacgata aacaagctca cgatttgcat tataatcttg aattgttttg
    38521 tattcttcat cacgtaatat acggcgattc aacttatcaa ccaaatgctc agttacttca
    38581 aatgtatcag cagtgatacc catatcctta attttcttat cagcttctac ctgtgaaatt
    38641 tctttaattt tagacgattg tttatgggtt ttatctccag aactcacacc gataactgtt
    38701 gcattattag gtttaatcca attgcttaca aattcatcat ccatatcatt agaaccaaag
    38761 aaaatatcac cacgcttaac attcactaag ctgatattgt tatcaatggt tccccaaggc
    38821 cagtctttac caattaaacc gcctacatca cctctagcaa catcaatagt acctttggct
    38881 acagaatttc ttacattaga ccaagcacca acatctcttg gattaaaatc atgagctaaa
    38941 tgtcctacta tagcacctgc acgagtattt aaacctttaa gttcaacatc tgcataagct
    39001 ttatttaaat tgcctcgata aatctcagat gcaatcccag ccaagttcca ccctttatca
    39061 ccaacagatt gaacttttcc gataaaagca acattagtca taatacctgc atcatctaat
    39121 ttatccaaaa tacccgcaac attgttatta ccaacgatag tacccgttac tttaatattt
    39181 tcaacagttg catttttcac tgtttgagct aatgaagcta attgatttgc ccaaggcata
    39241 ttaatattaa cgttaattaa attaatattt ttgatagaac ccccttcaac tctatcaaat
    39301 aatggacgag cagtgttatg aatggagaaa tgcttaccat caacacttgt taagtgacct
    39361 gtaaatttac ctgttacata tgatttactt ggtgtaggta catttgttgc atttaaatcc
    39421 gatcctaatt taaactcacc gttaggattt ttctgcatag catctactaa atctttgaag
    39481 ttatagtaaa cgttattttg cgatgctttg ggtttctcta tgtaataagt gtatgtttga
    39541 ctcaaactat tatttgaatc acgttggatt aaatcaggag ctgaagcagt aactttaaat
    39601 aaggatgtct cctcctcagt tacttcctct atattagtaa tggcaagcct tgttacttta
    39661 ttatgattag tggtaatttt tagataatat gacgttagat tttctggaac agtttctaga
    39721 aggctactat ctgtttcatt tccattctca tccactttca tcaatcttgt ttctttaata
    39781 tctttaatct caactttttt aaggtctaac tgaacttctt tatcttctaa tatttcagtt
    39841 tcttcaccgt tacctcgatc atagaccatg gttgtggata gtgtataacc ttgataatat
    39901 ttcaagttgt ctaaagtagc ttgtaagtta ttttcttcta ccgttacttc tttaacaact
    39961 gttccagtat tatcttttag agctacttta atcgatttaa tttctactcc agctggttta
    40021 gtcaatgtat aactagctgt cgcactcttc ttcaagacat ctttatctgt tttagcccat
    40081 tccaaggttg gtttctctaa ctcttttgtg ccattcttaa taattttgtc ttgaggtttt
    40141 tcaatcactg tttctgtgat agtaggagcg tcacttgttt tttctccgcg aattgtttta
    40201 taggtttcag taatttgttt cttatcgttt acaccttctt gagttacttg tcttgttccg
    40261 cgttttaaag tgtcatcttc ttgttctttt gtttcaaaag gaatgatttc ttctcttgtc
    40321 tcaaccgttg tcccttcaat tggttttgta ccacggatta cctgttgatt tacaggctcc
    40381 ttggtgactt ccgtactggt tgaaagaact tggtcagttt taacccctcc tattgtttta
    40441 taggttgttt ttgtaacttg acttcctttt tcaccatttc gtaggacagt ttcttcgtcc
    40501 gtgtacttgg ttgggtcctc agtcgtttca gttttatagt caagttctgt agttgattct
    40561 tcaacgagtg ttccttcagt caccttatac tcaggtaatt gttctggaac aagagcctca
    40621 cccacgtaat cttcttcttg cgtcccctta gctgttttag tctcctcttt tggttctaca
    40681 gtttcttcag ccttggtatc tggagttgac tcttcttgtt tcggtgtctc ttccgctccc
    40741 ctatcttctt taggattgtc ttcttgtgat gattcttttg gtgtacttgg ttgctcagct
    40801 tgtgtaggtt gcacgggttg ttctgggact tttgcttgtt ctactggtgg ttcaactttt
    40861 gattcctcag ctggtttgtc tgatggttga ctttctggct taactgctac tttttcctct
    40921 gattttgact caacttctcc acctacttct tcaactggag ctggttctgc tgaatcttct
    40981 ttcccctctt ctactttagg aagggtgtcg tcagtaggtt ttacctccga ttttggttct
    41041 tcctttggac tttcttctgt tctaggtgct tcttcttttg gagtttcctc tgtctctact
    41101 acttggtttt ctgtcctagc ttgctcctga tttgttgttg attgaggagt ctcaacttcg
    41161 actacagtca cctctccagg ttttgctgag gtttcttcta aaacagtgtc caagccaagc
    41221 gttttgagga tgtcacctga tagataacca acatagcgat agccatccat ttcaacaaca
    41281 ccctctcgac tagccagcgc taaggtcgca actgggtcta cagcccctgc actaggaaga
    41341 actaccaatc ccatagctcc aactagaaag acgctagcaa ttttctttct cttgtagatt
    41401 aaaagcaagc tcccaacagt cagcaaacca aaagctgtca aaacagatgc ttctgtccct
    41461 gtttgaggca actgatcttt ttgatacacc aaaccatata caacttcatt cctgtcaggc
    41521 tttcctgtct gaattaaatc tttagcttct tgtgaaataa tctctttatt tacatagtga
    41581 taggtagctg cgtccactac agaaggagcc atcaaaaggc ttccaagaaa tacagagcct
    41641 acaactccct taatcttacg aattgaaaaa cggtcttttt taaacacttt catctccttt
    41701 attcattctc aagactttct aatagcatct tgcggatagt gcgcacgcgc acctccgatt
    41761 aattttggac gactagccag tgccgttaca tgggcatgac caatctctct caaaataggg
    41821 cgaatcggaa cctgaacatg cttgacatgc atgccaattg cagtgtctcc gatatccaat
    41881 ccagcatgag ccttgataaa ttcaacctca actggatcct gcataaactt aaaggctgcc
    41941 aactgccccg aacctcctgc atgaagagta ggatggacac tgacaatttc cagatcaaac
    42001 tgctctgcca cctgacgttc aacaacgaga gcccgattga catgctcaca accttgaact
    42061 gctaaatgga tacctctact acctagaata cccaagatag tctccactat cagctcacca
    42121 atctcttgac tggattcttt cccaatctga ccacctagca cctcactaga agatagacct
    42181 aaaacaaaaa ggaccccctg cttcaaattg gtcttttcta aaacatcttc cactacctga
    42241 cgtgtttctc tttgaatctg tgtctcgttc atctctgtta cctctgttgt cactcttcta
    42301 tcataccgtt ttttcttgtt tttagcaaga tagacaacct ataaagtctg cccaattacg
    42361 cataaaactc ccagaattga ctgggagtta gctagtttct attctattta tatatatttc
    42421 aactttcgtt cctttttggg gtctagaatc aatcttcatc tggtaattgg ctccaaaatg
    42481 aagtttgagc cgttgatcga cattttgaag accaactccc ccacgtttga gttgactttg
    42541 actactatca ccagcatctt ggaagccaac gccatcatcc tcaatacgga tgaccaatcc
    42601 cgaatcctgt ttctggacag aaagtttaat atggccctga ccttcctttt ccttaatgcc
    42661 atggtaaaga gcattttcta caaggggttg taggaccagc ttgggtaaga ctaaattatc
    42721 aaaggcaaca ttttcattaa tttcgtattc cagcttatct ccatagcgtt gtttctggat
    42781 aaagagatac tggcggacat gattgatttc gtcagagaga caaatcaagt ccttgccttg
    42841 attgagcgcc aagcggaaat aggttgccaa ggacttggtc acctgcacca ctcgctgact
    42901 atcatgaaat tcagccatcc agatgatggt gtccaaagtg ttatagagga aatgtggatt
    42961 aatctggctc gaaagggctt gaagttggta ctgacgggtc gtttcttcct ggctacgaat
    43021 agctaccatc aactgatcaa tctgatccaa catagcatta aattggcgag ttacttctct
    43081 cagttcatag gcaccaactt ccttggcacg aagattttga gcaccagaag caatttccaa
    43141 catggtttct ctcaaatcct tcaaaggagc aatccagcgt ttaagactga accacactaa
    43201 gcagagacaa acaagaagag atgtgacact ggccccaagc aaggtccaca agagctgact
    43261 ccgaacctgg tctaactttt ccaatgatga cacgccaagt accgtccaat cagttcctgc
    43321 aatcttctct tgactgacgt aggatttgtg accaggagta taaccctgac ctgtctcgat
    43381 gtagggtttc atagcctcca ttttgctaga cgaactataa actgtgtgtt gaggatggta
    43441 gacaaattca tggttttcat tgataatgaa ggcaaagccc tgctgcccca actggagttg
    43501 attgagatag gcttccagag tttcataaga aatatccaaa cgaagcacac caagattggc
    43561 tccctttgca tcaacaagtt cttgagtgac agaaatgacc cactgactat ctgatttacg
    43621 agctggagtc aaaacaggca tagctccctg atgaatagcc ttttggtacc aatcctcagc
    43681 catcatatca gaggaagttt tcatctgcac actgtcatct gtagaaatga cctgaccaga
    43741 tttggtcacc agcacaacag ttttcaagtc cttatctgac ttcaagatgg tcaaaaacaa
    43801 atctcggatt ccctcgacct tgtcttgact gggattctca gcataggcca gaacatccgt
    43861 ctgctgggtc aaaccagtcg aggtggtttc tagttttttg atataagact gaataaagtg
    43921 gctagtctgg ctgatggtcg tttggctgtt gccctcaatg gtggcctcaa tggctgaaga
    43981 acttgattga tagtagaaag ttccaaccag agctaggaga atgagaaaga ccagaaagat
    44041 ggaaataacc attctaacta aaagagaaga acgcttcatc ggtcttctcc cttcttaaac
    44101 tgacgaggtg tcacacctgc aatctgctta aaacgttggg taaaatagtt catatcttca
    44161 aaaccaacct tctctgcgat ctcataaatc ttcagatctg tagttaaaag caagagcttg
    44221 gcttgtttaa cacgttctct caccagataa tcctgaaaag gcaagcccaa ctctttctta
    44281 atcaaggaac tcagataggt cggactaaaa cctaagtcac tggctaaaga ctttaaacta
    44341 aattggctat cagccagatg agactggatt ttctgggcca tgtttccttc aaacctatta
    44401 gtcaataaat cttgtaactg ctcttctttc tcttccttgt ctagtttttg tttgattttc
    44461 cccaacattt cctcaatatc ctgacgagaa aagggtttga gcaggtagtc gtccacacct
    44521 agtttgacag cagacaaggc ataatcaaaa tcatcgtaac ctgttaaaaa gaccaaatga
    44581 acctgaggat aggtttctcg taccagactg gccaactgga tgccatttag atgaggcatg
    44641 ttgatatcgg ttaaaatgat atctggcacc tgcttttgga tcaattccca agcctgcctt
    44701 ccattttcag cctgaccgat gatttccata tcgtaggctg ctacattgac cagtttagtc
    44761 aaaccttgtc ttaccagata ttcatcttct acgattaaga ttgtgtaggt catgctctgc
    44821 tcctttacca cttactagta tcagtatagc aaaattctcc tctaactgct taggaaagac
    44881 ctcttatact caataaaaat caaaaagtaa actaggaaga tagccacagg tt
//
LOCUS       DAVBHS010000002        41263 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVBHS010000002 DAVBHS010000000
VERSION     DAVBHS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41263
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.002"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(134..337)
                     /locus_tag="VQ651_000045"
     CDS             complement(134..337)
                     /locus_tag="VQ651_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2753104.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(330..1412)
                     /locus_tag="VQ651_000046"
     CDS             complement(330..1412)
                     /locus_tag="VQ651_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET2753105.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1538..2320)
                     /locus_tag="VQ651_000047"
     CDS             complement(1538..2320)
                     /locus_tag="VQ651_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET2753106.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2320..3663)
                     /locus_tag="VQ651_000048"
     CDS             complement(2320..3663)
                     /locus_tag="VQ651_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET2753107.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(3762..5078)
                     /locus_tag="VQ651_000049"
     CDS             complement(3762..5078)
                     /locus_tag="VQ651_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HET2753108.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5271..6492
                     /locus_tag="VQ651_000050"
                     /pseudo
     CDS             5271..6492
                     /locus_tag="VQ651_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
     gene            6691..8265
                     /locus_tag="VQ651_000051"
     CDS             6691..8265
                     /locus_tag="VQ651_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2753109.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            8529..9317
                     /gene="codY"
                     /locus_tag="VQ651_000052"
     CDS             8529..9317
                     /gene="codY"
                     /locus_tag="VQ651_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HET2753110.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            9317..9892
                     /locus_tag="VQ651_000053"
     CDS             9317..9892
                     /locus_tag="VQ651_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET2753111.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDFFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            9974..10059
                     /locus_tag="VQ651_000054"
     tRNA            9974..10059
                     /locus_tag="VQ651_000054"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10008..10010,aa:Leu,seq:aag)
     gene            10342..11472
                     /gene="ugpC"
                     /locus_tag="VQ651_000055"
     CDS             10342..11472
                     /gene="ugpC"
                     /locus_tag="VQ651_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET2753112.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            11973..12731
                     /locus_tag="VQ651_000056"
     CDS             11973..12731
                     /locus_tag="VQ651_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET2753113.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGLSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMKLSGMIYDEGWLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            12818..13330
                     /locus_tag="VQ651_000057"
     CDS             12818..13330
                     /locus_tag="VQ651_000057"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET2753114.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            13512..14456
                     /locus_tag="VQ651_000058"
     CDS             13512..14456
                     /locus_tag="VQ651_000058"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HET2753115.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            14465..15142
                     /locus_tag="VQ651_000059"
     CDS             14465..15142
                     /locus_tag="VQ651_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HET2753116.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            15240..15998
                     /locus_tag="VQ651_000060"
     CDS             15240..15998
                     /locus_tag="VQ651_000060"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET2753117.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            16036..17541
                     /gene="lytC"
                     /locus_tag="VQ651_000061"
     CDS             16036..17541
                     /gene="lytC"
                     /locus_tag="VQ651_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HET2753118.1"
                     /translation="MSVTFFIGEERLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAESDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTGTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPHYSGKKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            18037..18555
                     /locus_tag="VQ651_000062"
     CDS             18037..18555
                     /locus_tag="VQ651_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET2753119.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            18685..19191
                     /locus_tag="VQ651_000063"
     CDS             18685..19191
                     /locus_tag="VQ651_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET2753120.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            19191..19361
                     /locus_tag="VQ651_000064"
     CDS             19191..19361
                     /locus_tag="VQ651_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753121.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            19393..20625
                     /gene="clpX"
                     /locus_tag="VQ651_000065"
     CDS             19393..20625
                     /gene="clpX"
                     /locus_tag="VQ651_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET2753122.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            20634..21221
                     /locus_tag="VQ651_000066"
     CDS             20634..21221
                     /locus_tag="VQ651_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HET2753123.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            21232..21612
                     /locus_tag="VQ651_000067"
     CDS             21232..21612
                     /locus_tag="VQ651_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET2753124.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            21664..22554
                     /gene="rapZ"
                     /locus_tag="VQ651_000068"
     CDS             21664..22554
                     /gene="rapZ"
                     /locus_tag="VQ651_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET2753125.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            22551..23528
                     /locus_tag="VQ651_000069"
     CDS             22551..23528
                     /locus_tag="VQ651_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HET2753126.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            23525..24436
                     /gene="whiA"
                     /locus_tag="VQ651_000070"
     CDS             23525..24436
                     /gene="whiA"
                     /locus_tag="VQ651_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HET2753127.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(24470..25438)
                     /locus_tag="VQ651_000071"
     CDS             complement(24470..25438)
                     /locus_tag="VQ651_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET2753128.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWTLAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            25574..26431
                     /locus_tag="VQ651_000072"
     CDS             25574..26431
                     /locus_tag="VQ651_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HET2753129.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            26418..27197
                     /locus_tag="VQ651_000073"
     CDS             26418..27197
                     /locus_tag="VQ651_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HET2753130.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            27221..28573
                     /locus_tag="VQ651_000074"
     CDS             27221..28573
                     /locus_tag="VQ651_000074"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET2753131.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAIQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            28647..29021
                     /locus_tag="VQ651_000075"
     CDS             28647..29021
                     /locus_tag="VQ651_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HET2753132.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            29023..29871
                     /locus_tag="VQ651_000076"
     CDS             29023..29871
                     /locus_tag="VQ651_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2753133.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            30148..30915
                     /locus_tag="VQ651_000077"
     CDS             30148..30915
                     /locus_tag="VQ651_000077"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET2753134.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDDMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            30927..32111
                     /locus_tag="VQ651_000078"
     CDS             30927..32111
                     /locus_tag="VQ651_000078"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET2753135.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            32108..33979
                     /locus_tag="VQ651_000079"
     CDS             32108..33979
                     /locus_tag="VQ651_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2753136.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDSKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(34122..35306)
                     /gene="mntE"
                     /locus_tag="VQ651_000080"
     CDS             complement(34122..35306)
                     /gene="mntE"
                     /locus_tag="VQ651_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HET2753137.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPISEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            35610..38306
                     /locus_tag="VQ651_000081"
     CDS             35610..38306
                     /locus_tag="VQ651_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET2753138.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            38319..39110
                     /gene="yghU"
                     /locus_tag="VQ651_000082"
     CDS             38319..39110
                     /gene="yghU"
                     /locus_tag="VQ651_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HET2753139.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            39226..39636
                     /locus_tag="VQ651_000083"
     CDS             39226..39636
                     /locus_tag="VQ651_000083"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET2753140.1"
                     /translation="MEKKIVKDILFLSQVSHPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGMQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            39648..41243
                     /locus_tag="VQ651_000084"
     CDS             39648..41243
                     /locus_tag="VQ651_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753141.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
BASE COUNT        12083 a         7731 c         9450 g        11999 t
ORIGIN      
        1 gataaattaa ttgtctaact gtttgggggc agtacatatt caacacctga tactatgcgt
       61 tttattgtgg gaagattttc ctcattttct agtgaaattg aactttttct aacatctttt
      121 gatagatgac ttcttaatca attatcattt ccattgttgt ttcttggggt ttcatccctt
      181 gtctttgata aaagcggaga gcaccctcgt tatcattcca gacacttagg gttagattat
      241 tgcagccaat ttctttagca taatagcaga aaaatgacaa taaaatagcc cctagatatt
      301 ttgaaaggaa agcatagggg ctatatagtt taatcaaaat aaagcaaatc tttgctggtg
      361 cttgtaaaga ggtcgaagcc atccttggta acaacaccgc agtcttcaat acgaacaccg
      421 actttaccag ggatatagat acctggttca acagagaagc acatgccttc ttcgatgacc
      481 atgtcgtttc cttccatgat agatgggaat tcatggacat ccataccgat accatgcccg
      541 agacggtggt tgaagtactc accataacca gctttttcga tgacctcacg ggcagcgcgg
      601 tccacttcat gagcagtcac acctggcttg ataaagtcaa gagcagcttg ttgggcttca
      661 agagtcaagt tgtaaatatc tttcttgaat tggtctggtt tgccgacagc gactgtacga
      721 gtcatatctg acgcatagcc attgaccaga acacccaggt caaagaggag aagagcatca
      781 ttttcaacct tattagctgc tggaatgccg tgtggattcg cagcattatc accagtcaag
      841 accatggtat caaagctcat ttcataacct tcacgtttca tggcaaagtc gatttgtgcg
      901 atgatatctg tctcagtctt atcaagagaa atattgtcaa aaccaacatg aacagccttg
      961 tcagcataaa gacctgcaac catcattttt tgcacttcat cagctgattt gatgaggcgc
     1021 atgcgttgga tacgaggagt gaggttgtca aactcagcag tctcaaaaac tgttttcaaa
     1081 ccatggtatt tggtcaagat gagattgtca aactcaacag cgacacgttt gaagtcaagt
     1141 tgtggaagag catgtttgat tttttgccat ggattttcag aatcgacata gcccactact
     1201 gggaaggaaa cggtgctact tgcacgttct acttcaagag ctgggacaaa gaggagaggt
     1261 tcctgatccg ctaggacaaa gaggaacatt tggcgttcat ggggatcact gtaaaaacca
     1321 gtgaggtaat tgattgtgac ggggtcagat acgacagcga cgtctagttt ttctgattca
     1381 agatatgtta ggatttgttg taatttagac atatgctacc ttctttctac ccctctattt
     1441 tggcaaaaaa tgagagaaaa tgcaagggag aagggtaaaa gaaaagacaa aaaactccga
     1501 caagataacg gagttctttg atatcatttt cctttgttta agaaaagtca gccttgcttt
     1561 tgacgtcact gtactcttca gcgatttctt ggctgagaat gtcctcatag gcagggagtt
     1621 ggatgtcctg accatatttc ttcttgaggg cagtagtaac taggcgataa gccagattgg
     1681 cattacgaga gaggataggc ccgtggaagt aggaaccaaa gacattctta taatgaaccc
     1741 cttcaccgac cttttcttcg ttgtttccat ttccatagac aacctgtccc agcggttttt
     1801 ggtcatcaga gaggaaggta cgaccttggt gattttcaaa tccatagtag gtttcatcga
     1861 aatcttcatt gtgaatcttg atgtcaccga taaaacggtt attggtctgg ttgagcgtgt
     1921 agtgtcccat gacccctagc ccttcgatac gttttcctga agcttcaaca taatattgac
     1981 ccaatagttg gaaaccaccg cagatagcca gaactacacc gtcgttttgg atgtagttgt
     2041 caatgctctc ttttttagca ggtaggtcgt ctgcaatgat actttgttca aagtcttgac
     2101 caccaccgaa aaaggcgatg tcgtagtgat tttcatcaaa gtcatcatgg agagaaacga
     2161 tgtcaacggt cacatgggct cccagttttt cagccacata cttgagcatg aggatgtttc
     2221 cattgtcccc gtaggtattc atgagatttc cgtagaggtg ggcaatgttg agctgatagg
     2281 ggtaattgct atcttttgag gaaagtgaag tataaaccat tagttcatct cctttctaac
     2341 aatctgacga ctagccagca gttcacgaaa ttccagcatg gcagtataag ttgccagaat
     2401 ataggcatgc ttgcagtctt gattctcaat ggtcttgaga acttgctcca gattactcgt
     2461 ttcagtgatt ttctcagctg gatagccagt cactcggagg cgacgagcga tttcagaatg
     2521 acgaacaccg ccagcgttga tttcaggaat gtccatgtca gtgatttgct caaagtctgc
     2581 atcccagatc cagctagtgt caattccatc tgcatagttg gcattaagga ggacagatag
     2641 gctaaatgga taaggtgcta gtttgatcat ttcgatagct tgggttgcac cgactggatt
     2701 tttaatcaag acaagggtac attccttgtc accgatatga aaggtttctt ggcgtccaaa
     2761 gacagcacgg ctcttgtcaa atccctgttt gatgagttgg gaatctgcgc cgaggaaacg
     2821 ggcgatggcc acagcagcta gggcgttata gatattatag agcccgccga tttggatacc
     2881 gtattcttgg ccgtctatga caaagcgaga gcgattgttg gtcaactcaa ccagttttgt
     2941 caaacgatag tcgagatcag gacgtttaca tccacaacct tcacagatat aggcacccaa
     3001 gtttgcatag gtattatgct catatttgag gatgccttgg cagtcaggac agagaatccc
     3061 ttcggtattg tagtgagcca gttgggctgg tcctttttcc aagtcaaaac caaaatactc
     3121 tatagggttt ggaatagttg gcttgtagaa aagtggactg tctccgttaa ggagaacagt
     3181 agcagttgga acttttcgaa tggcatccaa tatcatgtta taggtagtat agatttcacc
     3241 gaaacggtcc atctggtcac ggaagatatt agtaatgaca aaaagactag gctggatata
     3301 gtcacagata cgagatagac tggcttcgtc aatttcgagg acggcaatat ttttcccagt
     3361 ttttgaagat ttggctgtta ggaaggttgt tgcaatccct gtaatcatgt tggcaccgct
     3421 tgggttggtt agaacttgac cataaacctc ttttaaaatg ccgacagtga gggcagttgt
     3481 cagggttttt ccatttgttc cagtgacaac gacaatctcg tagttcttag ctaggctttg
     3541 taaaatatct ttatcaaatt gaagggcgac tttccctggg agcgtacttc cacgtccaag
     3601 acggcttaaa acgaagtggg aagaacgccc agcaagaagg cccaaagtag tttttaagtt
     3661 catgtttcta ttatatcaca aaagagggaa aagacagatt tggtgtttcg tagaagtaaa
     3721 aacagcccaa agagggctgt cagttaagat gagatttcaa cttaaatcgc aaacaagtca
     3781 ttcaagttct cagccaaggt tgggtgagtg aagatttgtt ttgtgaagta agtgtaagga
     3841 atcttgttgt ccatagcaac agtaatgatg ttgatgattt cttgagaacc ttctgagaag
     3901 atgcttgcac caagaatttc ttttgtttca gtattaacaa cagctttgaa agctccgcga
     3961 aggtctccat ttacgtgacc acgaggcatg gctgcaacag ggatttcttt cactgcgtat
     4021 ggaagtttca aatcagctgc ttggctttca gtcaaaccaa cttgtgaaag tgcaggtgtg
     4081 atgaacatag tatttggcac attgagacgg tcctcaagtg tgtagctgcc atctccagca
     4141 aggtagctgt aaacaacacg gaagtcatca agtgaaatgt aagtaaattg aaggccaccg
     4201 ttgacatctc caactgcaaa gacaccagga acgtttgttt gacagtgttt gtctacttta
     4261 atagcaccac gttcagttag ttcaatatct gtattttcaa gttgaagtgg ttctacattt
     4321 ggtttgcgtc cagttgcgta gagaagggcg tcgaaacggt aagtttcgtc ttcagttacg
     4381 acaagcactt ggtcaccatc gtttttgatt tcagtagtat ggatattttg aagcaattca
     4441 atgccatctt cttccatgta ttgtttagca agagctgcga tggaaggttc tgcacgaggt
     4501 aggaatgtat ccaaggcatc taggactgtg accttgcttc caagtttgtt gtaaaggccg
     4561 gcaaattcaa gaccgatatt tccgccacca aggattccaa gtttttcagg taatttgtcc
     4621 aagctttgga tacctgttga gtcaaagatg tttttgcttg tagcaagtcc aggaattggc
     4681 aagacgtttg aaacagcacc agtgttgatg acgattgttt cagcagtcag ttctttcttt
     4741 tcatcaccag cttggatttc gatgacttta tttgaaagga agtgagcttc cgcatcaaag
     4801 atatctacgc ctgtaccagc aacagtcgca tagtttttac cgttgaggcg accagtgatc
     4861 gtgtttttag tagcaatcac ttcttcaaaa gacaagtcct tttcagcagc aactagcaag
     4921 gttttagttg ggatacaacc aatgttgata caagttccac cgtacatagc cttgctgcgt
     4981 tcaacgaggg caactttttt gccagctgaa gccaatttac ctgctagtgt tttaccagct
     5041 ttaccaaatc caataacaat taaatcgtat gttaacattt atcttcctcc tattcgaatt
     5101 tcattctagc acaagaaaaa aacttttgca caattctgct acggaaaaga cagaaaaaga
     5161 taaaaacgct ttccagacaa aaaactagta attttttgac aagcttgata aactaaaatc
     5221 ctatctattt ttatataaga aagaataaat gttcgaaaat aggaggtttt atgaaatcga
     5281 atcgttatat tattgccttt gctggggtca ttttacactt aatgctgggt tcgacttatg
     5341 cttggagtgt ttatcgtaac cctattattg aaaaaacggg atgggatcag gcttctgttg
     5401 ccttcgcctt tagtctagca atcttttgtt tgggcttatc ggctgcattt atggggcgtt
     5461 tggtagaaaa atttggtccg aaagtcatgg ggagtctatc tgcttttcta tacgcaggtg
     5521 gaaatatctt aacaggattt gcaatagacc gtcaggaact gtggttgttg tatctagctt
     5581 atggcatttt aggtgggctt ggtttgggag caggctatat tacccctgtg tcaacgatta
     5641 taaaatggtt tcctgataaa cgtggtctcg caacaggttt agcgattatg gggtttggtt
     5701 ttgcttcttt attgactagt cccatagcgc aacacctcat cgcaggggta gggattgtag
     5761 aaacttttta tattttagga gcaagttact ttattatcat gctcctagct tcacaattca
     5821 ttaagcgtcc aaatgagcaa gagcttgcaa ttttatcttc ttcagggaaa gaaaaaacag
     5881 cctctttgac gcaaggaatg gctgcaaatc aggctctaaa aagcaatcgg ttttatatgc
     5941 tttggattat tttctttatc aacatagctt gtggtttagg cttaatttca gcggcatcgc
     6001 caatggcaca ggagatggct ggcttgtcta caagtcatgc agcagtaatg gtgggtgttt
     6061 tggggatttt caatggattt ggtcgcttgc tctgggcgag tttgtctgac tatatcggtc
     6121 gccctctaac ctttagtata ttactgcttg tcaatctttt ctctctcact ttggctcttt
     6181 acagattccg ttttatttgt agttgctatg tctattttga tgacttgcta tggagctggt
     6241 ttttctttga ttccagctta tctcagtgat atttttggaa ccaaggaatt ggccgctctg
     6301 catggttata ttttaacagc ttgggcaatg gctggtttag cgggacctat tttattagca
     6361 gagacttata aaatggctca ttcgtacaca caaaccttgt tcgtttttct cattttatac
     6421 agtatcgcct tggctttgtc ttattatcta ggtcgttcaa tcaaaaaaga aagtcaaaaa
     6481 ccgcttacat gattttctgt agaaatgggg ctatcttttg ctctggtctt gtgttatact
     6541 agataggttg caaagaaaac agtacttttc ttttgtggaa aagaagcaac acgattttat
     6601 atctagttat atgaaaatac gtcataaaaa gaaaagtata aactaagccc tatagggtgg
     6661 cttcgcacca cctttagaaa gaagaataac gtgaaattta atgaattaaa cttgtctgct
     6721 gatttgctag cagaaattga aaaagctggt tttgtagaag ctagtcctat ccaagaacaa
     6781 actattcctt tggcccttga aggcaaggat gttatcggtc aagctcagac tggtacagga
     6841 aaaactgcag cctttggctt gcctaccctt gaaaaaatcc gtacagaaga agcgactatc
     6901 caagctttgg tcatcgctcc aactcgtgaa ctagctgtcc aaagtcaaga agaactcttc
     6961 cgctttggtc gtagtaaggg agtcaaagtc cgttcagtat atggcggatc aagcattgaa
     7021 aaacaaatta aggctcttaa atctggtgcc catatcgtgg tgggaactcc aggtcgcctc
     7081 ttggacttga ttaaacgcaa ggccttgaaa ttacaagaca ttgaaaccct tatccttgac
     7141 gaagcggatg aaatgcttaa catgggcttc cttgaagaca tcgaagccat tatttcccgt
     7201 gtacctgaga accgtcaaac tttgcttttc tcagcaacta tgccagatgc catcaaacgt
     7261 atcggtgttc agtttatgaa agcccctgaa catgtcaaga ttgcggctaa ggaattgaca
     7321 acagaattgg ttgaccagta ctatatccgt gttaaggaac aagaaaaatt tgacaccatg
     7381 actcgtctca tggatgtggc acaaccagaa ctcgctattg tatttggtcg taccaaacgc
     7441 cgtgtggatg aattgactcg tggtttgaaa attcgtggct tccgtgcaga aggaattcat
     7501 ggcgacctag accaaaacaa acgtcttcgt gtccttcgtg actttaaaaa tggcaatctt
     7561 gatgttttgg ttgcgacaga cgttgcagcg cgtggtttgg atatttcagg tgtgacccat
     7621 gtctacaact acgatattcc acaagatcct gagagttatg ttcaccgtat cggtcgtaca
     7681 ggtcgtgctg gtaagtcagg tcaatctatt acttttgttg ctccaaacga aatgggttac
     7741 cttcaaatca ttgaaaactt gactaagaaa cgcatgaaag gtctcaaacc tgcaagtgta
     7801 gaagaatcct tccaatcaaa aaaacaggta gctctcaaga aaatcgaacg tgattttgca
     7861 gatgaaacca ttcgtgccaa ctttgagaaa tttggtaagg atgctcgcaa attggctgct
     7921 gagtttactc cagaagaatt ggcaatgtat atcttgagtc tgacagtcca agacccagat
     7981 agccttccag aagtggagat tgcacgtgaa aaaccactac cgtttaaacc atcaggtaat
     8041 ggtttcggtg gtaaagctaa gggaggtcgt ggaggccgtc gtggggacga ccgtcgagag
     8101 cgtgatcgcc gtggcaatgg tcgccgtgat gagttcaaaa aaggaagtcg tggcaacgat
     8161 cgttttgata aggaaaaacg ttaccgtaag gataataaaa aaccacgcaa tactttaagc
     8221 gaaaagcaaa caggctttgt tattcgtaac aaaggcgata aataagaaaa agcggttcaa
     8281 aatgaatcgc ttttttatat aaggagaccg aatgtgaaaa gacaaataaa attactatat
     8341 tatcttttat aatgttatga tacacaaaaa taggcaactt gtcaatagaa aaggaacaat
     8401 ttaattaaaa atattctggt ttttaattaa attgtaagtc aatgcaagtc aataaggaat
     8461 tttcagataa ttattgaaaa agagacgttt ttatgttata atgatagcga ttaaattcga
     8521 ggtgaaaaat ggcacattta ttagaaaaaa ctagaaaaat tacatcaatt ttgaaacgct
     8581 cagaggagca gttgcaggat gagctccctt acaacgctat tacgcgtcag ttagcggata
     8641 ttattcattg caatgcctgc attatcaata gtaagggacg tctgcttggc tattttatgc
     8701 gttataaaac aaatacagat cgcgtagagc aattcttcca aactaagatt ttcccagatg
     8761 actacgttca aggggctaat atgatttacg aaacagaagc aaacttgcct gttgagcatg
     8821 atatgagtat tttccctgtt gagagtagag atgattttcc agatggcttg acgactattg
     8881 caccgattca tgtatcgggg attcgccttg gttctttgat tatttggcgt aatgataaaa
     8941 aattcgaaga tgaggacttg gttcttgttg agattgccag taccgttgtt gggattcagc
     9001 ttcttaactt ccaacgtgaa gaagatgaga aaaatattcg tcgtcgtact gctgtcacca
     9061 tggcggtcaa taccctttct tactccgaac tccgtgctgt ttcagcaatt ttaggggaat
     9121 taaatggaaa tgaagggaag ttgactgcgt cagtgattgc agatcgtatc ggaatcactc
     9181 gctctgtgat tgtcaatgct cttcgtaaac ttgagtctgc ggggattatt gaaagtcgct
     9241 cacttggaat gaagggaacc tatcttaagg tcttgatttc agatattttt gaagaagtga
     9301 agaaaagaga ttactaatga caaaggcttt aatttcgatt gattatacag aagattttgt
     9361 tgctgatagt gggaagctga cagcaggtgc tccagctcag gcgatttcgg atgccatcag
     9421 caaggtaact cgattagctt ttgaacgagg agattatatc ttctttacta ttgatgctca
     9481 tgaagaaaac gattttttcc atccggaaag taagttattt cctcctcata atctgattgg
     9541 gacgagtgga cggaatttat atggagattt ggggatcttt tatcaagagc atggttcaga
     9601 cagtcgtgtc ttttggatgg ataaacgcca ttactcagct ttttcaggga ctgacctaga
     9661 tattcgtttg agagagcgta gagtgtctac cgttatctta acaggtgtct tgacggatat
     9721 ctgtgtccta catacagcta tagatgccta taatctagga tatgacatcg agattgttaa
     9781 accagctgtt gcttccatct ggcctgaaaa tcatcaattt gccctaggtc atttcaaaaa
     9841 tacactcgga gctaagttag tagatgaaaa tctaaatgaa ctttctgagt aacctggttg
     9901 gtgaaaatga aaaaatttga aaaaaagtgt tgacaagcat cccaaaagtt gatatactag
     9961 taaagtaatc gatgcgggga tggcggaatt ggcagacgcg caggactaag gatcctgtga
    10021 ccgctttagg tcgtgagggt tcaagtccct ctctccgcat aggaaaagag ttagctaaag
    10081 ctagctcttt tgtatttctc taaaaaatta gaatccagtc aaatgtagtg gagaatcagc
    10141 gaaaatatgg gaaaactgtt ccttgtttct gcttttgtca tagaagaata ataaagatta
    10201 tcttcttcaa attctccgat attctctaaa gttttgtgca agttgcacag aactttttta
    10261 tttttttggt catcttgcca tagaaatata aagcgttttc atctataata taattatcaa
    10321 aagacaaaag gagttcacct catggtagaa ttgaatctta aaaatattta caaaaaatat
    10381 ccaaacagcg aacactattc agttgaagat ttcaacttga acatcaaaga taaagaattt
    10441 atcgttttcg taggaccttc aggatgtggt aaatcaacta cactccgtat gattgctggt
    10501 cttgaagaca ttacagaagg tactgcatct atcgatggcg tggttgtcaa cgacgtagct
    10561 ccaaaagacc gtgatatcgc catggtattc caaaactacg ctctttaccc acacatgact
    10621 gtttatgaca acatggcttt cggtttgaaa ttgcgtaaat acagcaaaga agacattaac
    10681 aaacgtgttc aagaagcagc tgaaatactt ggattgaaag aattcttgga acgtaaacca
    10741 gctgaccttt caggtggtca acgtcaacgt gttgccatgg ggcgtgcgat tgtccgtgat
    10801 gcgaaagtat tcttgatgga cgaacctttg tcaaacttgg atgccaaact tcgtgtatca
    10861 atgcgtgctg aaatcgctaa aattcaccgt cgtatcggag ctacaactat ctatgtaact
    10921 cacgaccaaa cagaagcgat gacacttgca gaccgtatcg ttattatgtc agctactaag
    10981 aaccctgctg gtacaggtac tatcggacgt gtagaacaaa tcggtactcc tcaagaagtt
    11041 tacaaaaatc cagttaacaa attcgttgca ggattcatcg gaagcccagc tatgaacttc
    11101 atcaacgtga aattggttgg tagcgaaatt gtttctgacg gtttccgttt gaaagtgcca
    11161 gaaggagcat tgaaagttct tcgtgaaaaa ggctacgaag gaaaagaatt gatctttggt
    11221 atccgtccag aagacgtgaa tgcagaacct gctttccttg aaacattccc agactgtgtt
    11281 gtaaaagcga ctatctctgt atcagaactg cttggttcag aatctcacct ttactgccaa
    11341 gttggtaaag acgagtttgt tgcaaaagtt gatgctcgtg actacttgca aacaggtgca
    11401 acagttgagc ttggatttga cttgaacaaa gcacacttct tcgatgtaga aactgaaaaa
    11461 acaatctact aaaataaata aaattcaaag cactacaaga aaagatatct ctttatcaat
    11521 tgtagtggag agatatcagt taatctaggg agagaaacaa aatgcttctc tcctttttgc
    11581 tagggaagtc atattatgca tctatattgt gatgctcttt aatactcttc gaaaatctct
    11641 tcaaaccacg tcaacgtcgc cttgccgtac gtatgattac tgatttcgtc agttttatct
    11701 gcaacctcaa agatgtactt tgagcagctt acggctagtt tcctagtttg ctctttgatt
    11761 tccattgagt attatttgtg ggtaccatct acaagtgaag ctatatgcgt aaactacgtg
    11821 agcaattgaa ttcgaactag agaggtaata ataaatttat gctatagtta tggtgacttg
    11881 tatgcttttg attctagttt atcaaataat agattagaat tgtcagataa tatcattttg
    11941 tgttataatg aagaaaaaac agaggtgttc aaatgtcaga agcaggtcat aagtttttag
    12001 caaaattggg gaaaaaacgc ttacgtccag gtggaaagcg tgctacagat tggttaattg
    12061 cagaaggagg attgtcaaaa gaaaagagaa tactagaggt tgcgtgtaat aggggaacta
    12121 cagcaattga gttggcacag cgttttggtt gcaagataac tgctgttgat atggatgctc
    12181 aagctttaga agtggctaaa aaatctgctg gaacggcagg tgttgctcat ttaatcagtt
    12241 ttgaaagagc aaatgcaatg aaacttcctt atcaagatgc tagttttgat attgttataa
    12301 atgaagctat gctgactatg caagccgatc aagctaagaa aaaatgtgta atggaatatc
    12361 taagggtatt aaaacctgga ggtcttcttt tgacacatga tgtgcttctt aaggaagcta
    12421 aagagtctat cagacaggaa ttatcacaag caattcatgt aaatgtaggt cctttaactc
    12481 aagatggttg ggaacaggtg atgatagaat caggttattg tgatgtgaaa gtattgactg
    12541 gtgaaatgac attaatgaaa ttatcgggta tgatttatga cgaaggttgg ctaggaactt
    12601 tgaaaatttg tgtaaatgct tgtaaaaagg agaatagaaa gcagttttta actatgtata
    12661 aaatgtttgc taagaataaa cagaaattgg gctttattgc gatggctagt tataaatcgt
    12721 caaaacgtta gataattatt gaagttaact tttccttttt tctttcttaa aaaatatgct
    12781 ataatagaga gtgaaaaact ttgaaagaaa gaaaaagatg aatttaaaag attacattgc
    12841 aacaattgaa aattatccaa aggaaggcat taccttccgt gatattagtc ctttgatggc
    12901 tgatggaaat gcttatagct acgctgttcg tgaaatcgtt cagtatgcta ctgacaagaa
    12961 agtcgacatg atcgtgggac ctgaagctcg tggatttatc gtgggttgtc cagttgcctt
    13021 tgagttggga attggttttg cgcctgttcg taagccaggt aaattgccac gcgaagttat
    13081 ttctgctgac tatgaaaaag agtacggtgt cgataccttg actatgcacg cggatgccat
    13141 taagccaggt caacgtgttc ttattgtaga tgaccttttg gcgacaggtg gaactgttaa
    13201 ggcaactatc gagatgattg aaaaacttgg tggtgttatg gcaggttgtg ccttccttgt
    13261 tgaattggat gaattgaacg gccgtgaaaa aatcggtgac tacgactaca aagttcttat
    13321 gcattattaa tgaaaacagt ccctagggct gttttctcta cactaggata taaaaataga
    13381 ctataactag ttagagaaaa actataattg aaaactatat cttcttgcag tataataaaa
    13441 ggactaagtg tttgagattt gtcttcaaac acatgcaatt attcctgaga gagtacagtt
    13501 aggagagggt tatgccgatt cgaattgata aaaaattgcc agctgttgag attttacgga
    13561 cagagaatat ctttgtcatg gatgatcaac gtgctgccca ccaagatatc cgtcctttga
    13621 agattttaat tttaaatctc atgccacaga aaatggtcac agagacccag ttgttgcgtc
    13681 acttggctaa tacaccccta caactggata ttgattttct ctatatggag agccaccgtt
    13741 ctaaaacaac tcgttcagag cacatggaga ccttctataa aacttttcct gaagtcaagg
    13801 atgagtattt tgatgggatg atcatcacgg gtgctccagt tgagcattta ccatttgagg
    13861 aagtggacta ttgggaggaa tttaggcaga tgcttgagtg gtctaagaca catgtctatt
    13921 cgacccttca tatctgttgg ggggctcagg ctgggcttta tctgcgctat ggtgtagaaa
    13981 aataccagat ggacagtaag ctatcaggta tttatcctca ggacacccta aaagagggtc
    14041 accttctatt tagaggcttt gatgatagct atgtatcccc tcattcacgg cacacggaga
    14101 tttctaagga agaggtctta aacaagacca atctcgagat tttatcagaa ggacctcagg
    14161 ttggggtttc tattttggcc agtcgtgatt tacgagaaat ttatagtttt ggtcatttgg
    14221 agtatgaccg tgataccttg gcaaaagagt attttcgaga tcgtgatgca ggtttcgacc
    14281 cacatattcc agaaaattac tttaaagatg atgatgttaa tcaggtacct tgtctttgtt
    14341 ggtcttcatc tgcagccctc tttttcagta attgggtaaa ccatgcggtc tatcaggaga
    14401 cgccttttga ttggagaaag atagaagatg atgcatctgc atatgggtat ttataagagg
    14461 aattatgaca tatttagacg cttttaaatc aggtaccttg gttttaccga gtgccctgct
    14521 cttgcatttt aaggaactct ttccttctag cgacgatttt ctggtttggc aatttttcta
    14581 tttgcaaaat acgacaggct tagaagaaat gtcgccaagc cagattgctg aaaggattgg
    14641 caaggaaatt tcggatgtca accagtccat ttctaatctg acggaaaggg gactgctcca
    14701 gtatcgtact atcgaattaa atggcgaaat tgaattgctc tttgatgcta gtttggcctt
    14761 ggaacgtttg gatgacctgt ttggagcagt tcgttcaagt tcagaccagc taacacctca
    14821 aaaccagctc aaggatttgg tggaaacctt ccagcaggag ttgggacgat tgttgacgcc
    14881 ttttgagatt gaggatttga ccaagacact aaaggaagat ggaaccagtg ctgacttgat
    14941 taaggaggct cttcgtgaag ctgttttgaa tggaaaacca aactggaagt acattcaggc
    15001 gattttgaga aactggcgcc atgaaggaat caagagtgtg gctcaaattg aggccaagag
    15061 agcagaaaga gaagcaagca atcctcagtt gacacaggta tctgcagatt tcagaaatgc
    15121 catggatctc tggaaggatt aatccatgca agtaggcttg aaatccgagt aagatttgca
    15181 agctgtgtat aattgtgata gaataaatag aaaataaatt gaaaaaagag gtatgtgaaa
    15241 tgtcacgtaa accatttatc gctggtaact ggaaaatgaa caaaaatcca gaagaagcta
    15301 aagcattcgt tgaagcagtt gcatcaaaac ttccttcatc agatcttgtt gaagcaggta
    15361 tcgctgctcc agctcttgat ttgacaactg ttcttgctgt tgcaaaaggc tcaaacctta
    15421 aagttgctgc tcaaaactgc tactttgaaa atgcaggtgc tttcactggt gaaactagcc
    15481 cacaagtttt gaaagaaatc ggtactgatt acgttgttat cggtcactca gaacgccgtg
    15541 actacttcca tgaaactgac gaagatatca acaaaaaagc aaaagcaatc tttgcgaacg
    15601 gtatgcttcc aatcatctgt tgtggtgaat cacttgaaac ttacgaagct ggtaaagctg
    15661 ctgaattcgt aggtgctcaa gtatctgctg cattggctgg attgactgct gaacaagttg
    15721 ctgcctcagt tatcgcttat gagccaatct gggctatcgg tactggtaaa tcagcttcac
    15781 aagacgatgc acaaaaaatg tgtaaagttg ttcgtgacgt tgtagctgct gactttggtc
    15841 aagaagtcgc agacaaagtt cgtgttcaat acggtggttc tgttaaacct gaaaatgttg
    15901 cttcatacat ggcttgccca gacgttgacg gtgcccttgt aggtggtgcg tcacttgaag
    15961 ctgaaagctt cttggctttg cttgactttg taaaataatc agtaagtagc aaaagctagg
    16021 tggaacagca ttcagatgtc tgttacattt tttataggag aggaaagatt gaaaacaaaa
    16081 attggattag caagtatctg tttactaggc ttggcaacta gtcatgtcgc tgcaaatgaa
    16141 actgaagtag caaaaacttc gcaggataca acgacagctt caagtagttc agagcaaaat
    16201 cagtcttcta ataaaacgca aacgagcaca gaagtacaga ctaatgctgc tgcccactgg
    16261 gatggggatt attatgtaaa ggatgatggc tctaaagctc aaagtgaatg gatttttgac
    16321 aactactata aggcctggtt ttatattaat tcagatggtc gttactcgca gaatgaatgg
    16381 catggaaatt actacctgaa atcaggtgga tatatggccc aaaacgagtg gatctatgac
    16441 agtaattaca agagttggtt ttatctcaag tcagatgggg cttatgctca tcaagaatgg
    16501 caattgattg gaaataagtg gtactacttc aagaagtggg gttacatggc taaaagccaa
    16561 tggcaaggaa gttatttctt gaatggtcaa ggagctatga tgcaaaatga atggctctat
    16621 gatccagcct attctgctta tttttatcta aaatccgatg gaacttatgc taaccaagag
    16681 tggcaaaaag tgggcggaaa atggtactat ttcaagaagt ggggctatat ggctcggaat
    16741 gagtggcaag gcaactacta tttgactgga agtggtgcta tggcgactga cgaagtgatt
    16801 atggatggtg ctcgctatat ctttgcggcc tctggtgagc tcaaagaaaa gaaagatttg
    16861 aatgtcggct gggttcacag agatggtaaa cgctatttct ttaataatag agaagaacaa
    16921 gtgggaaccg aacatgctaa gaaaatcatt gatattagtg agcacaatgg tcgtatcaat
    16981 gattggaaaa aggttattga tgagaacgag gtggatggtg tcattgttcg tctaggttat
    17041 agcggtaaag aagacaagga attggcgcat aacattaagg agttaaaccg tctgggaatt
    17101 ccttatggtg tctatctcta tacctatgct gaaaatgaga ccgatgctga gagtgacgct
    17161 aaacagacca ttgaacttat aaagaaatac aatatgaacc tgtcttaccc tatctattat
    17221 gatgttgaga attgggaata tgtaaataag agcaagagag ctccaagtga tacaggcact
    17281 tgggttaaaa tcatcaacaa gtacatggac acgatgaagc aggcgggtta tcaaaatgtg
    17341 tatgtctata gctatcgtag tttattacag acgcgtttaa aacacccaga tattttaaaa
    17401 catgtaaact gggtagcggc ctatacgaat gctttagaat gggaaaaccc tcattattca
    17461 ggaaaaaaag gttggcaata tacctcttct gaatacatga aaggaatcca agggcgcgta
    17521 gatgtcagcg tttggtatta agcgatgatt tgaaagaggg atgtgatagt agcaccctct
    17581 ttttctttgt tttatgatag ttcatcctcg agtaaattca agttcttgct cggaaatgaa
    17641 gcttatatag tagattgaat atagacaaat accttgtgat tggtaaaaca ttttagaaat
    17701 tcatttacct ttcctaatcg acttggtttc atcttatttc aatctattat agtattgggg
    17761 aatttcttca aaccacatca gcttggtcag ttctacctgc gacctcaaaa cttgtgcttt
    17821 ggtcaagctg ggtttagttt cctagtttgc tgatggattt ccattgacta taagcatcca
    17881 accctctttt tgtcttctaa agaattctta aattatcagt ctattgcaac ttttctcata
    17941 taagttcttt gtcttgctat tggttttcct tagtagtata ctaaggtagt aatcattaag
    18001 aagtggttac aaaaaataat gaatgaggta aagaaaatgg tagaattgaa aaaagaagca
    18061 gtaaaagacg taacatcatt gacaaaagca gcgccagtag cattggcaaa aacaaaggaa
    18121 gtcttgaacc aagctgttgc tgatttgtat gtagctcacg ttgctttgca ccaagtgcac
    18181 tggtatatgc atggtcgtgg tttccttgta tggcatccaa aaatggatga gtacatggaa
    18241 gctcttgacg gtcaattgga tgaaatcagt gaacgcttga ttacactcgg tggaagccca
    18301 ttctctacat tgacagagtt ccttcaaaat agtgaaatcg aagaagaagc tggtgaatac
    18361 cgtaatgttg aagaaagctt ggaacgtgtt cttgttatct accgttactt gtcagaactt
    18421 ttccaaaaag gtttggatgt cactgatgaa gaaggtgacg atgtgacaaa cggtatcttt
    18481 gcaggcgcta aaactgaaac agataaaaca atttggatgc ttgcagccga acttggacaa
    18541 gcacctggtt tgtaagaaat aaggaaacat agataaatct gtaggaaatt tcttacagat
    18601 tttttctatt ttgtaagcta ttccaaacca gtcttttttt gagttttttg ataaaatagt
    18661 actatcagtg aaaaggatgg aagcatgact aagaaaatcg tagctatttg ggcccaggat
    18721 gaagagggtg tgattggtaa ggaaaatcgt ctgccttggc atttgccagc agaattgcag
    18781 cactttaaag aaacaactct gaatcatgct atcttgatgg ggcgtgtgac ctttgatggg
    18841 atggggcgtc gcttgcttcc aaaacgggaa accttgattt tgacgcgtaa tccggaagaa
    18901 aagatagatg gggttgctac ttttcaggac gtccagtctg ttcttgactg gtatcaggct
    18961 caagaaaaga atctctacat tatcggtggg aagcaaattt ttcaggcttt tgaaccttac
    19021 cttgatgaag tgattgtcac tcacattcat gctcgggtgg aaggagatac ctatttccct
    19081 gaagaacttg acttgtctct ttttgagaca gtttcaagca aattttacgc caaagatgag
    19141 aagaatcctt atgattttac catccaatac cgcaagagaa aggaagtcta atggaacgga
    19201 gtttatttgg tctattcacg gcttttctgt gtttcatctg ctttctggca ggagcacaag
    19261 cttttcgtaa aaagcgttac gggctttcta tcctcctttg gttaaatgcc tttaccaatc
    19321 tggtcaatag tattcatgct ttttacatga ccttatttta gatagaatga cagtatagaa
    19381 tggaaggaaa tcatgtctac aaatagaaaa aatgatatga tggtttattg ctcattttgt
    19441 ggcaaaaacc aagaagaagt acaaaaaata attgctggca acaatgcttt tatttgtaat
    19501 gaatgcgtgg agttagctca ggaaatcatt cgagaagaat tggttgagga agtcttggca
    19561 gacttgtctg aggtgccaaa accaattgaa ctcctccata tcttgaacca ctatgtaatt
    19621 ggtcaagatc gtgccaagcg tgccttggca gtggcggttt ataaccacta caaacgcatc
    19681 aatttccacg atacacgcga agagtcagaa gatgtggatt tgcagaagtc aaacattttg
    19741 atgattggcc caactggttc agggaaaact ttccttgccc agaccttggc taagagcttg
    19801 aatgtacctt ttgctattgc ggatgcgaca gctctgacgg aggctggtta tgtgggtgag
    19861 gatgtggaaa atatactcct caaactcttg caggttgctg actttaacat cgaacgtgca
    19921 gagcgtggca ttatctatgt ggatgaaatt gacaagattg ccaagaagag tgagaatgtg
    19981 tctatcacac gtgatgtttc tggtgaaggg gtgcaacaag cccttctcaa gattattgag
    20041 ggaactgttg ctagcgtacc gcctcaaggt ggacgcaaac atccacaaca agagatgatt
    20101 caagtggata caaaaaatat cctcttcatc gtgggtggtg cttttgatgg tattgaagaa
    20161 attgtcaaac aacgtctggg tgaaaaagtc atcggatttg gtcaaaacaa taaggcgatt
    20221 gacgaaaaca gctcatacat gcaagaaatc atcgctgaag acattcaaaa atttggtatt
    20281 atccctgagt tgattggacg cttgcctgtt tttgcggctc ttgagcaatt gaccgttgat
    20341 gacttggttc gcatcttgaa agagccaaga aatgccttgg tgaaacaata ccaaaccttg
    20401 ctttcttatg atgatgttga gttggaattt gacgacgaag cccttcaaga gattgctaat
    20461 aaagcaatcg aacggaagac aggggcgcgt ggacttcgct ccatcatcga agaaaccatg
    20521 ctagatgtta tgtttgaggt gccgagtcag gaaaatgtga aattggttcg tatcactaaa
    20581 gaaactgtcg atggaacgga taaaccgatc ctagaaacag cctagaggtg actatggaac
    20641 ttaatacaca caatgctgaa atcttgctca gtgcagctaa taagtcccac tatccgcagg
    20701 atgaactgcc agagattgcc ctagcagggc gttcaaatgt tggtaaatcc agctttatca
    20761 acactatgtt gaaccgtaag aatctcgccc gtacatcagg aaaacctggt aaaacccagc
    20821 tcctgaactt ttttaacatt gatgacaaga tgcgctttgt ggatgtgcct ggttatggct
    20881 atgctcgtgt ttctaaaaag gaacgtgaaa agtgggggtg catgattgag gagtacttaa
    20941 cgactcggga aaatctccgt gcggttgtca gtctagttga ccttcgtcat gacccgtcag
    21001 cagatgatgt gcagatgtac gaatttctca agtattatga gattccagtc atcattgtgg
    21061 cgaccaaggc ggacaagatt cctcgtggta aatggaacaa gcatgaatca gcaatcaaaa
    21121 agaaattaaa ctttgaccca agtgacgatt tcatcctctt ttcatctgtc agcaaggcag
    21181 ggatggatga ggcttgggat gcaatcttag aaaaattgtg aggaaaagaa aatggcaaaa
    21241 acaattcata cagataaggc cccaaaggct atcggaccct atgttcaagg aaaaatcgtt
    21301 ggcaaccttt tgtttgctag cggtcaagtt cccctatccc ctgaaactgg ggaaattgta
    21361 ggagagaata tccaagaaca gacagagcaa gtcttgaaaa acatcggtgc tattttggca
    21421 gaagcaggaa cagactttga ccatgttgtc aaaacaactt gcttcttgag cgatatgaac
    21481 gactttgttc cttttaatga ggtttaccaa acggccttca aagaggaatt cccagctcgt
    21541 tcagctgtgg aggtagctcg tcttcctcgt gatgtaaaag tcgaaattga agtcatcgca
    21601 gagattggat aagctagttg aagtttggtg ttgccaaact tcttttgata taaggagaaa
    21661 aagatgacaa agaaacaact tcacttggtg attgtgacag ggatgagtgg cgcagggaaa
    21721 actgtagcca ttcagtcctt cgaggatcta ggttatttca ccattgataa tatgccgcca
    21781 gctctcttgc ctaagttttt gcagctggtt gaaattaagg aagataatcc taagttggct
    21841 ttggtagtgg atatgcgtag ccgttctttc ttttcagaga ttcaagctgt tttggatgag
    21901 ttggaaaatc aagatggttt ggatttcaaa atcctctttt tggatgcggc tgataaggaa
    21961 ttggtcgctc gttacaagga aaccagacgg agtcacccac tagcagcaga cggtcgtatt
    22021 ttagatggaa tcaagttgga acgtgaactc ttggcacctt tgaaaaatat gagccaaaat
    22081 gtggtggata cgactgaact cactccacgt gaactgcgca aaacccttgc agagcagttt
    22141 tcagaccaag aacaagccca gtctttccgt atcgaagtca tgtctttcgg atttaagtat
    22201 ggaatcccga ttgatgcgga cttggtcttt gatgtccgtt tcttgccaaa tccctattat
    22261 ctaccagaac tgagaaacca aacgggtgtg gatgaacctg tttatgatta tgtcatgaac
    22321 catcctgagt cagaagactt ttatcaacat ttattggcct tgattgagcc gattctgcca
    22381 agttaccaaa aggaaggtaa gtccgttttg accattgcta tgggatgtac gggtggacaa
    22441 caccgtagtg tggcatttgc taaacgcttg gcgcaggact tatccaagaa ttggtctgtt
    22501 aatgaagggc atcgcgacaa agaccgcaga aaggaaacgg taaaccgttc atgagaaaac
    22561 caaagataac ggtgattggt ggagggactg gaattcccgt cattctaaaa agtctgcggg
    22621 aaaaagatgt ggaaatcgca gctatcgtga cggtggcaga tgatggtggt tcttcaggtg
    22681 aactccgaaa aaatatgcaa cagttgacac cgccaggtga tcttcgtaat gtccttgtgg
    22741 ccatgtcgga tatgcctaag ttttatgaga aggtctttca gtatcggttc tctgaggatg
    22801 ccggagcctt tgctggccat ccattgggaa atctcatcat tgctggcttg tcagaaatgc
    22861 agggttcaac ctataatgcc atgcagttat tgagcaaatt tttccataca acagggaaaa
    22921 tttatccttc cagtgaccat cctttgaccc ttcatgcagt ctttcaggat gggacagaag
    22981 tggctggaga gagtcatatt gtagaccatc gaggcataat tgacaatgtc tatgtgacca
    23041 atgccctaaa cgatgatacg cctctggcca gccgtcgagt agtgcagacc atccttgaaa
    23101 gtgacatgat tgtcctaggg ccaggttccc tctttacctc tattttgccc aatatcgtga
    23161 ttaaggaaat tgggcgggct cttttggaaa ctaaggcaga aattgcctat gtctgcaata
    23221 tcatgaccca acgtggggag acggaacact ttacagatag cgaccacgtg gaagtcttgc
    23281 atcgtcacct tggtcgccct tttatcgaca ctgtcttggt gaatattgaa aaagtgcctc
    23341 aggaatacat gaattccaac cgttttgatg aatacttggt gcaagtggaa cacgattttg
    23401 taggtctttg taagcaagtt tcgcgcgtga tttcatctaa cttccttcgt ctggaaaatg
    23461 gcggtgcctt ccacgatgga gatttgattg tggacgagtt gatgcgcatt atacaggtga
    23521 aaaaatgagt ttcacagtag cagtaaaaga agaaatcctg ggccaacacc atctgagccg
    23581 gcatgaatta tctgccatta tcaaaatgtc tggtagcatc ggtctctcga cttcgggctt
    23641 gactttgtca gttgtgacag aaaatgccaa actggcccgt cacctctatg agtcctttct
    23701 ccatttctat gaaatcaaat ctgaaattcg tcaccaccaa cggagcaatc ttcgcaagaa
    23761 tcgcgtctat accgttttta cagatgaaaa ggtgcaggat ttgttaagtg atttgcactt
    23821 ggcagactct ttctttggcc tggaaacagg tattgatgag gcgattttat cggatgagga
    23881 agcaggtcgt gcctatctct gtggcgcttt cttggcaaat gggagcattc gtgaccctga
    23941 gtcaggcaag taccagttgg aaatcagttc tgtttatctg gaccacgcgc aaggaattgc
    24001 ctcccttctc caacagtttt tactggatgc caaggtgctt gagcgcaaga agggggctgt
    24061 gacctatctc cagcgagcag aagacattat ggacttcttg atagtaatcg gagccatgca
    24121 ggcacgtgat gattttgagc gggttaagat tttgcgagaa acccgtaacg acctcaatcg
    24181 ggctaataat gccgagacag ctaatatcgc tcggacagtt tctgccagca tgaagaccat
    24241 taacaatatc agtaaaataa aagatatcat gggcttagaa aatttgccag tagatttgca
    24301 ggaagtggcg caactgagga ttcagcaccc agactactct atccagcagt tagcagatag
    24361 tctcagcacc cctctgacca aaagtggtgt caatcacaga ctcagaaaaa tcaataagat
    24421 agcggatgaa ttataaaacc acgaatccta tgtgactcgt ggttcttttt tataaactgg
    24481 tagagtgttt tggttgtact ttttgttcag ggtcaatgta gttgatagcg ttgttgacag
    24541 cagttggagc ttctccgagg cctgtcgcaa tcagatcaat ttttccgtca tagtagcagc
    24601 agtcaccgat agcatagata cctgcttggc tggattcctg tttgctgttg acgataatct
    24661 tgtgacggtt gaggtcgagc ccccagtttt taaggttacc gacagaagat ttgaaaccat
    24721 agttgacaaa gaggtggtct aggtcaatgg tttcagtttc atcagatttg acttttgtga
    24781 tttcaagttt atcaagtgtt tttccatttc caaggagttg gctaggggcg aatggtgtct
    24841 tgatggttac agatgattct tgcaaggctt gaacactgtg ttccaaggca cggaaattat
    24901 ctctgcggtg aacaagggta gttggtgcga ttttttcaaa agccaaagtc caatccacag
    24961 ccgagtctcc cccaccaaga atcgtcactt ttttaccagc gtattgctga atgttagaaa
    25021 cgtggtagtg gatattttca tagccctcaa ccccttcaag ttccagcgga cgtggtttga
    25081 aggcaccgcc acccatagcg atgataactg ttttagtcag gtgacttcct ttagaagttg
    25141 tgatggcaaa ttcttcttct tgtttgtcaa tctcaagaac cgtttcattg agatgaatag
    25201 gggtatcaaa tccatttagc tgttcaatca agcggttagt caactcttct ccagtcaggt
    25261 ttgggaagcc tggtacgtct aggatttcct tttcagggta gagaatagca ggttgtccac
    25321 ctagctgggg aagagagtcg atgatttgaa ccttggcttg gcgtaggtgg gcataaaagg
    25381 ctgcaaaaag cccgacagga ccaccaccca caatggtaat atcatagagt tgagacatag
    25441 tttctccttt gttttttcta gtcagtttat tttatcatat ttttgttcaa tgtaaaatgg
    25501 actaggatag ggaaaaaaat gccagaaaat ggtataatag gacgaaagtg ttttgatagg
    25561 gaggatatca gggatgaatt ttcaacaatt atccaatttg caatattgga ccagtttgtt
    25621 tgcaagtcca tggacgatag ctatcaatct gattgatatt ttgattgttg cttatatttt
    25681 ataccatttt acaaaagcta ttgcaggaac caagattatg attttggtac gtggagtttt
    25741 ggtatttatt ttagctcaaa tccttgcaaa tatgattggt ttgactacga tttcttggtt
    25801 aatcaatcaa attattactt atggggttat tgcggcggtt gttatcttct ctccagagat
    25861 tcggactggt ttggaacgtt tgggaagagc gacagatttc ttttccaatg cccctattag
    25921 tgctgaggaa cagatgattc gtgcctttgt taagtctgtt gaatacatga gtcctcgtaa
    25981 aatcggggcc ttggttgcta ttcagcgtgt acgtaccttg caggagtata tttcgacagg
    26041 aattcccttg gatgctaaga tttctgcaga acttctcatt aacattttta ttcccaacac
    26101 tcccctacat gacggtgcgg tgattatcaa agaagaacgt atcgctgtga cgtctgccta
    26161 tctgcccttg acaaaaaaca cagggatttc caaggaattt gggaccagac accgggcggc
    26221 tatcggttta tcagaagtct cagatgcctt gacttttgtc gtatcagagg aaacgggagg
    26281 aatttcgata acctataatg gaaggtttaa gcacaaccta acacttgatg aatttgaaac
    26341 agaattacgt gaaatcttac ttccaaaaga ggaagtaggt cttagtttta aagaacgatt
    26401 gctaggagga tggaaacatg aaaaaaaata gtttatatat catatcctca ctcttttttg
    26461 cttgtgtctt atttgtctat gctacggcga cgaattttca aaacagtacc agtgctaggc
    26521 aggtaaaaac ggaaacctat actaatacag taacaaatgt ccctattgac atacgctata
    26581 atagtgataa gtattttatt agcggttttg cttcagaagt atcagtggtc ttgactggtg
    26641 caaatcgcct atcgctagct agtgaaatgc aagaaagtac acgtaaattc aaggttactg
    26701 ctgacctaac agatgccggt gttggaacaa ttgaagttcc tttgagcatt gaagatttac
    26761 ccaatgggct gaccgctgtg gcgactccgc aaaaaattac agtcaagatt ggtaagaagg
    26821 ctcagaagga taaggtaaag attgtaccag agattgaccc tagtcaaatt gatagtcggg
    26881 tacaaattga aaatgtcatg gtgtcagata aagaagtgtc gattacgagt gaccaagaga
    26941 cattggatag aattgataag attatcgctg tcttgccaac tagcgaacgc ataacaggta
    27001 attacagtgg ttcagtacct ttgcaggcaa tcgaccgcaa tggtgttgtc ttaccggcag
    27061 ttatcactcc gtttgataca ataatgaagg tgactacaaa accagtagca ccaagttcaa
    27121 gcacatcaaa ttcaagtaca agcagttcat cggagacatc ttcgtcaacg aaagcaacta
    27181 gttcaaaaac gaattaaaaa tagaaaagga ttttataaca atgggtaaat attttgggac
    27241 tgatggagtc cgtggagaag ctaacctaga actaacacca gaattagcct ttaaactagg
    27301 acgttttgga ggctatgttc ttagtcaaca tgaaacggaa gcgccgaaag tctttgtagg
    27361 acgtgacaca cgtatttcag gggaaatgct agaatcggcc ttggtggcag gtctcctttc
    27421 agtagggatt cacgtataca aacttggtgt ccttgcaaca ccagcagtag cttacttggt
    27481 tgaaactgaa ggagcaagtg ctggtgtcat gatttctgct agccacaatc cagctcttga
    27541 taacggaatc aagttctttg gcggtgatgg cttcaaacta gatgatgaaa aagaagcaga
    27601 aattgaagcc ttgctagatg ctgaggaaga cactcttcct cgtccaagtg cagaaggctt
    27661 aggaattttg gtagattatc cagaaggctt gcgtaagtat gaaggatacc ttgtttcaac
    27721 tggaactcct cttgatggaa tgaaggttgc cttggataca gctaatggag cagcttctac
    27781 cagtgcccgt caaatctttg cagaccttgg tgcccaattg acggttatcg gggaaacacc
    27841 agacggtctt aacatcaacc ttaatgttgg ttcaacacat ccagaagccc ttcaagaagt
    27901 ggtcaaagaa agtgggtcag ctattggttt ggcctttgat ggagacagtg accgcttgat
    27961 tgctgttgat gagaatggtg acatcgtcga tggtgacaag attatgtaca tcatcggaaa
    28021 atacctttct gaaaaaggac aattggctca aaatacaatt gtgacaactg ttatgtctaa
    28081 ccttggtttc cacaaggcct tgaatcgcga aggtattaac aaggcagtta ctgcagttgg
    28141 tgaccgctac gttgttgaag aaatgagaaa atcaggttac aaccttggtg gtgaacagtc
    28201 tggtcacgtt atcttgatgg attacaatac cacaggtgat ggtcaattat cagcaattca
    28261 attgactaaa atcatgaagg aaactggtaa gagcttatca gagttggcgg cagaagtaac
    28321 gatttatcca caaaaattag ttaatatccg agtggaaaac gtcatgaagg aaaaggccat
    28381 ggaagtgcca gctatcaagg ccatcatcga gaagatggaa gaagaaatgg cggggaacgg
    28441 ccgtatcctt gttcgtccaa gtggaacaga gcccctcttg cgtgttatgg cagaagcgcc
    28501 tacaacagaa gaagtggact actatgttga tactatcaca gatgtagttc gtgctgaaat
    28561 tgggattgac taaatcctag aaaactagat gaaaataaaa aattatggta caatagctta
    28621 taatattgta gccgatggag aaagacatga atcttaaacg tgaacaagaa tttgttagtc
    28681 agtatcattt tgatgcacgt aattttgaat gggaaaatga aaatggagct cctgaaacca
    28741 aggtggatgt gaatttccaa ttgctccaac atgaccaaga aaatcaagtg acttccttga
    28801 ttgtcatttt gagctttatg attgtatttg ataagtttgt cattagtgga acaatctcac
    28861 aagtaaacca tatcgatggc cgtattgtca atgaaccaag tgaattgaac caagaagagg
    28921 tggaaacctt ggctcgtcca tgtttgaaca tgctcaatcg tttgacttac gaagtgactg
    28981 aaattgcatt agacttacca ggaattaatt tggagttcta atatgaaatt agctgttttc
    29041 acagattcct ctgcttatct cagtgaagag accttgcaaa gagaagactt gtttgtcttg
    29101 gatattcctg tcaatattga tggtgaggaa tatgtcgaag gcatcaatct gtctgctgag
    29161 gaattttacc aaaaaatggc tcaggcttct gaattgccta agaccagtca accaagtatt
    29221 gccaagttag atgagatctt aacttcgctc aaagaacaag gctatacaca tgccttgggg
    29281 cttttcctat cttctggaat ttcaggtttt taccaaaata tccagtatat ggtcgatgac
    29341 tatgagggct taaccattgc tttcccagac actttgatta caagtgctcc cctaggcatc
    29401 atggttgaaa gcgtctttaa ttggcgtgat cagggcgatg attttgccag cattcaggat
    29461 aagctagcca ttcaaatcag ccgtacgtca gcctttatca tggtagatga cttggatcat
    29521 ttggtaaaag gtggacgcct ttcaaatggg gctgccattt tgggcaattt gcttagcatt
    29581 aagccaatcc tttattttaa cgatcaaggt gtgattgaag tttacgaaaa agttcgtact
    29641 gaaaagaagg ccaccaagcg cttaattgaa attatcaagg aaacaacggc ttcaggtcaa
    29701 taccgggtca ttgtcattca tgggaatgct cctgaaaagg ctgaagaatt gcgtcagcac
    29761 ttgcttgatt ttggcttggg ttcggatgtt tcacttgcta catttggtag tgtcattgga
    29821 acgcacctag gagcaggaag tattgctctg ggttatattc cagtgattta gttggcgctt
    29881 ctaggctagt taagatgcct tgtatcttag aaattgaaca cggactacct gcctcttgaa
    29941 aaaagatgcc tgtcttgcct ttcgtggaaa gtcagtgcca ttccctattt ttcagaggca
    30001 gctaaggtcc tttgtatctt gataattgaa cacgcctgga accctgtgtg aaaaagatag
    30061 ttcttccaag aagtagacac ttcttgattg gaattcctat tttcactttg tgttcttacg
    30121 gactttgtat cttagacagg agtagagatg agtattcgag taattattgc cggttttaag
    30181 ggaaagatgg gccaggctgc ttgtcagatg gtattgactg atccagactt ggacttggtg
    30241 gcagttttgg atccttttga gtctgagtca gaatggcagg gtattcctgt tttcaaggat
    30301 aaggctgatt tagctggttt tgaagcggat gtctgggtag attttactac tccagctgtt
    30361 gcctacgaaa atacacgttt tgctcttgaa aatggctttg ctccagtagt tggaacgact
    30421 ggtttcacga gtgaagaaat tgcagagcta aaagaatttt ctcgtgccca agacttgggt
    30481 ggcctgattg cccctaactt tgccttgggt gctgtcttac tcatgcaatt tgcgacgcag
    30541 gctgccaaat atttcccaaa tgtggagatt attgagctcc atcatgacaa gaaaaaggat
    30601 gctccgagtg gaacagccat taaaacagct gagttgatgg cagaggttcg agagtccatt
    30661 cagcaaggcg cagcagatga ggaagagctg attgctggtg ctcgtggtgc tgactttgat
    30721 gatatgcgca tccactcagt tcgtttgcca ggcttggtag ctcatcagga agtcatcttt
    30781 ggcaatcagg gagaagggtt gaccctccgt catgactcct atgatcgcat ctccttcatg
    30841 acaggagtca atttgggaat taaagaagtt gtcaagcgtc atgagcttgt ctatggatta
    30901 gaacacttat tatgagatta acgcaaatgc cttctgaatt tcagaaggct ttaccagtat
    30961 tagaaaaaat taaagaagca ggctttgagg cttattttgt tgggggctct gttcgagatg
    31021 cccttctcca tagccctatc cacgatgtgg atattgcgac gtcttcttat ccagaagaga
    31081 ccaagcagat ttttccgcga acagccgata tcggaatcga gcatggaacc gtcttggtct
    31141 tagatgggga tgaggagtat gaggtaacaa cctttcggac agaggatgtc tatgtggact
    31201 atcgcagacc cagtgcggtt tcctttgtac gatcgctaga agaagacctc aaacgccgtg
    31261 atttcacagt caacgccttt gccttggatg agacaggaga aatcgttgac ttgttccatg
    31321 gtttagaaga tttggaaaag caagtcttgc gagcagttgg agtggctagt gagcgtttca
    31381 acgaagatgc tttacggatt atgcgtggtt tccgttttca ggctagtctt ggttttgcac
    31441 ttgagccaga aacatttaaa gctatgaaga ccttgacgcc gcttttggag aaaatttctg
    31501 tagagcgtac cttcgtcgag tttgataaac tcttgctggc tccattttgg agaaggggct
    31561 tggcttccat gattgagagt caagcttatg actatctccc tgatatggca tctagccagg
    31621 acaagctcaa cagactgttt gatttggaga ctgattttac ttttgaatcc tctgaacaag
    31681 cctgggcggc tttactatgg gctttggaga ttgaaaatgc gcagtcattt ttgaaatctt
    31741 ggaagacctc acgccagttt gccaagcaag ttcaggattt gctgattatt ttggctctgc
    31801 gtgaaaatgg agaattgagt aagcgagatt gttatcgctt tgacatagat ttgcttttac
    31861 aggctgaaaa tcttcgtcaa gctcaaggaa aagaagtcaa cccacaagcc atcacagaaa
    31921 aataccaaag cttgactatc catgacaaga aagagattca gattaatggc gatattttga
    31981 tcaaggaata tggctatcag ccaggcccag acttgggaga gattttaaca gagattgagt
    32041 ttgccattgt cgatggagaa ttggagaata atcgtgaagc catccatgct tacctgaggg
    32101 agaaaaaatg agtgatttta tcgttgaaaa actaagtaaa tccgttggtg acaagaccgt
    32161 ttttagggat atttccttta ttatccatga cttagacaga attggtttaa tcggtgtcaa
    32221 tgggactggc aagaccaccc ttttggacgt cctttctggt gtttctggat ttgatgggga
    32281 tgtcagtcct ttttcagcta aaaatgatta ccagattggt tacttgactc aggatcctga
    32341 ttttgatgat agaaagacag ttttggatac ggttctatct agtgaactca aggaaatcca
    32401 gctcattcgt gagtatgaat tgattatgct cgactatagt gaggacaagc aggcgcgttt
    32461 ggaacgtgtc atggcagaga tggactctct ccaagcttgg gaaatcgaaa gtcaggtcaa
    32521 gaccgttctt agcaaattgg gcattcaaga cttatctact cctgttgggg aattgtcagg
    32581 tggtctgaga agacgggtac agttggcaca agtcttactt ggcaaccacg acctcttgct
    32641 tttggatgag ccgaccaacc atctggatat tgcgattatt gagtggctga ccctcttttt
    32701 gaaaaattct aagaagaccg tcctttttat cactcacgat cgttatttct tagacgcttt
    32761 gtcaacacgg attttcgagt tggatcgtgc aggcttgacc gagtaccagg gcaattacca
    32821 ggactatgtt cgcctaaagg cggaacagga tgagcgcgac gcggctcttc ttcacaaaaa
    32881 agaacaactc tacaaacaag aactggcctg gatgcgcaga caaccgcagg cgcgtgcgac
    32941 caagcagcag gcccgtatca atcgattcca tgatctgaaa aaggaagttt caggcagtag
    33001 tgctgagaca gacttgacca tgaactttga aaccagtcgg attgggaaga aagtcatcga
    33061 gtttcaggct gtttcctttg cctatgaaaa caagcctatt ttgcaagatt ttaatctctt
    33121 ggtgcaggcc aaagaccgta tcggaatcgt tggggacaat ggtgttggaa aatcaaccct
    33181 gctcaacttg attgcaggaa gtcttgagcc aactaaagga caagttgtga ttggggaaac
    33241 tgttcgcatc gcctatttct ctcagcaaat tgagggtttg gatgaaagca agagagttat
    33301 caattacctg caggaagtgg cagaagaggt caagaccagt ggcggttcta cgacttcgat
    33361 tgcagagttg ctggagcagt tccttttccc acgttcgacg catggaacct tgattgagaa
    33421 attgtcaggg ggtgagaaaa aacgtcttta tctcctcaaa ctgcttttgg aaaaaccaaa
    33481 tgttcttctt ttagacgagc caaccaatga cctagatatt gcaactttga cagtcttaga
    33541 gaatttcttg caaggttttg caggtcccgt tttaacagtc agtcacgacc gctatttctt
    33601 ggataaggta gcgaccaaga ttctcgcttt tgaggatagc aagattcgtc ctttctttgg
    33661 tcattacacc gactatcttg atgaaaaagc ttttgaaaca gatatggcca atcaagtgca
    33721 aaaggtcgaa aaggaaaaag tggtcaaggt tcgagaagac aagaaacgta tgacctacca
    33781 agaaaagcag gagtgggcaa gtattgaagg tgatattgaa accttggaaa aacgtatcgc
    33841 tgctattgaa gaggaaatgc aggctaacgg ctctgacttt ggtaagctgg ctactctcca
    33901 aaaagaattg gatgagaaaa atgaagcact ccttgaaaaa tacgaacgct atgagtatct
    33961 cagtgaattt gatagttaag actattcgaa aatcaaagtg caaactatgt cggcttcaca
    34021 ttgccgactc aagtacagcc tgtgaccggc ttcctaattt gcactttgat ttttattgag
    34081 tataaaagaa tagaaaaatc ccgtctcatg aaaggatttc tctattcttt tttggtttct
    34141 tgatgaaaga tattttgcca ggtttcgtgg cgacgccaga tactggtatg gatgatacca
    34201 tctaactcat agcagatgag tttggttttt tgactgatgg aagtaatttg aatgtccttg
    34261 atagcagaat ttaactcttt tttggtctta taagcctctt tatccatctg ctctccatct
    34321 tggcgaatat agacaaaatc atcagtcaag agttcttcta gttggtttcc ttggtcaatc
    34381 aattgttcac gcataagcaa ttttttatag acattgtcta aaatttcatc ctcagagata
    34441 ggtgctggtt cgatatggac atcggtatca aagacgccaa aacgctcctc cagcatagac
    34501 tcgacctgat ccgcgatttc atggctttca aaaacagaca agtcaggatt catctctagt
    34561 gtaatatcca ggtagatgtt gctaccgtag gtgcgacctc tttgcgattt gaccttgctg
    34621 attttgggaa tttccatgat agccttttgg tagtcctcga gcaggcggtc gtcaaagcca
    34681 tctgaaagac taaaggaaga ctcgatgaag atatcatagg cagtcttcaa gataaagaaa
    34741 gtgatgatga tagcaaccag tttatccaca atcggataat tgaaactact agctaggatg
    34801 gcaatggcgg ttccaagtga ggtaacagcg tcagaaagat tgtccttagc agctgccttc
    34861 agcgccttgg agttggattt cttactgagg cgagtattgt agagatagac cacaaacata
    34921 atcgctgcag aaatgattcc tagagttgca ccaagaggat caatgaccgt ttcttcccga
    34981 ctgagaatct tttgaatggt atctcttaga acatcgaaac cgacatagaa catgatgata
    35041 gaagtgatca agcttgccaa atcttcaatc ttccaatgac caaaacggtg gtcacggtct
    35101 gcaggctggc gcgccatccg aatcccgatt aagagggcca catttccaat gatgtccgat
    35161 acgttattaa aaccatcggc caccaaactg gatgaatgaa ggagatgacc agctgctaat
    35221 ttggctgcag acaagatcaa ataggtcgaa atactgataa tggctccacg ctcagctaac
    35281 ttgagatttg agatagattg cttcattcaa cttcctttct agtcacatag cattctacca
    35341 ttatactggt tttcaaggaa aaattcaaat aaggcagttt ttactctttg aaaactcccc
    35401 aaaatttggt ataatggtac tactcaaggg agtagctggc agaaacctgt gatagtgtcg
    35461 tcattccgaa ttttatgctg aaaagtatgc tttccggccc tatcttaaac agcgagactt
    35521 gttatgatta acaggtctct ttttgtttgt cgtgaaaaga tatgacagaa agagagtctg
    35581 ttttgcacac aatgtcaagg aggagacaca tgtcaaaaga acaaaaacgc caagcgtttt
    35641 atactcagag ccctgaagag gtcttgcagg ctgtggatgc gaccgagcaa ggtttgtcat
    35701 caagtgaggc ggaaaagcgc cttgctgaat ttgggcacaa tgaactcgaa gaaggcgaga
    35761 aacgatcaat cttggtcaaa ttcatcgagc aatttaagga tttgatgatt atcatcctag
    35821 ttgcggcagc aatcttgtca gtcgtgactt ctggtgggga agatatcgca gatgccatta
    35881 tcatcctagc tgtggttatc atcaacgctg cctttggtgt ttaccaagaa ggaaaagctg
    35941 aagaagctat tgaagccctc aaatccatgt ctagtccagt tgcccgcgtt cttcgtgatg
    36001 gacacatggc agagattgac tctaaagaat tggtaccagg cgatatcgtt gcccttgaag
    36061 caggtgacgt ggtaccagcg gacctacgtt tgatagaagc caactctctt aaaattgaag
    36121 aagcagcctt gacaggtgaa tctgtaccag tcgaaaaaga cttgtcagtc gagcttgcga
    36181 cagatgctgg tattggtgac cgtgtcaaca tggccttcca aaactcaaac gtaacctatg
    36241 gtcgtgggat gggtgttgtt gtcaatacag gtatgtacac tgaagttggt catattgctg
    36301 gtatgcttca agatgcggat gagactgata caccactcaa acaaaatttg aacaaccttt
    36361 ctaaggtctt gacctatgct atcttggtca ttgcccttgt tacttttgta gtgggtgtct
    36421 tcattcaagg gaaaaatcca cttggtgagt tgttgacttc tgttgccctt gccgttgcag
    36481 ccattccaga aggacttcct gctatcgtta ccatcgttct ttctcttggt actcaagttt
    36541 tggccaaacg acattccatc gttcgtaagt tgccagcagt tgaaacactt ggttcaactg
    36601 aaatcatcgc ttctgataag actggtacgc tgactatgaa caagatgaca gtcgaaaaag
    36661 tcttctacga tgcggttcta catgactcag ctgatgatat tgaactaggt cttgaaatgc
    36721 cactacttcg ttcagttgtc ttggccaatg atacgaaaat cgatgtggaa ggtaacttga
    36781 ttggtgaccc aaccgaaaca gcctttatcc aatatgcctt ggacaagggc tatgatgtca
    36841 aaggtttctt agagaaatat cctcgtgtag ctgaattgcc atttgactct gaccgtaagc
    36901 tcatgtcaac agttcaccca ttgccagatg gtcgtttcct tgtagcagtc aagggtgcgc
    36961 cagaccaact cttaaaacgt tgtcttcttc gtgataaggc tggggatatt gctccgattg
    37021 atgagaaggt tacaaatctc attcatacaa acaattctga aatggctcat caagccttgc
    37081 gtgtccttgc aggtgcttat aagattatcg atagtattcc agaaaatctc acttctgaag
    37141 agcttgaaaa tgatttaatt tttactggtt tgattgggat gattgaccct gaacgtcctg
    37201 aagccgctga ggctgttcgt gtggctaagg aagcgggaat ccgtccaatt atgattacag
    37261 gtgaccacca agacacagcg gaagccattg ccaaacgttt gggaatcatt gacgcaaacg
    37321 atacagaagg tcacgtttta actggtgctg aactcaatga actgtcagat gaagaatttg
    37381 aaaaagtcgt tggtcaatac tctgtttatg cccgtgtgtc tccagaacac aaggttcgta
    37441 tcgtcaaggc ttggcaaaaa caaggtaaag tcgttgccat gacaggtgac ggtgtcaatg
    37501 acgcgccagc tctgaaaaca gccgatatcg gtatcggtat gggaatcact ggtacagagg
    37561 tttctaaggg ggcttctgat atgattcttg cagatgataa ctttgcgact attatcgtcg
    37621 cagtggaaga aggacgtaag gtcttctcaa acattcaaaa gactattcag tacctacttt
    37681 ctgctaatac tgctgaagta ttaaccatct tcctatcaac cttgtttggt tgggatgtct
    37741 tacagccggt tcatcttttg tggattaact tggtaacgga taccttccca gctatcgctc
    37801 ttggtgttga acctgcggaa cctggtgtca tgaatcataa accacgtgga cgcaaggcaa
    37861 gcttcttctc aggtggtgtt ttgagttcta tcatttatca aggtgtactc caagcagctc
    37921 ttgttatgag tgtttatggc cttgcgattg cttacccagt tcatgtgggt gacaatcatg
    37981 ctattcatgc agatgcccta acgatggcct ttgcaaccct tggtttgatt cagctcttcc
    38041 atgcctacaa tgtcaagtct gtctaccaat ccatcttgac agttggccca ttcaagtcta
    38101 agacctttaa ctggtccatc ttggtatcct ttatccttct catggcaaca atcgttgtag
    38161 aaccgcttga aggaatcttc cacgtaacca aactagactt gtcacaatgg ggaattgtta
    38221 tggctggaag cttctcaatg attatcatcg tcgaaatcgt taagtttatt caacgcaaac
    38281 tcggttttga caagaatgcg atttaaggga ggtttcggat gtcagcatat caattaccga
    38341 ccgtatggca ggatgaagct agtaatcaag gagcttttac ggggctaaac agaccaacag
    38401 caggtgcccg tttcgaacaa aacttgccaa aaggagaaca agcttttcag ctttattcac
    38461 tgggaacacc aaatggtgtg aaggttacta tcttattgga agaattacta gaagctggtt
    38521 ttaaggaagc ggcttacgac ttgtataaga ttgctatcat ggatggggat caattcggct
    38581 cagactttgt gaagctcaat ccaaattcca agattccagc cttattggac cagtcaggta
    38641 ctgaaaacgt aagagtcttt gagtctgctc atattcttct ttaccttgct gagaaatttg
    38701 gagccttttt accaagtaat cctgtggaaa aggtagaagt tttgaattgg ctattctggc
    38761 aagcaggtgc agcacctttt ctaggtgggg gatttggaca tttcttcaat tatgctcctg
    38821 aaaaattgga atatcctatt aaccgtttta cgatggaagt gaaacgccag ttggatttat
    38881 tggataagga attggctcag aaaccttata ttgcaggcaa tgactatacg attgcagata
    38941 ttgctatctg gtcttggtat ggacagttag ttcaaggaaa tctttaccaa ggttctgcaa
    39001 aattcttgga tgcctcaagt tatcaaaatc tagtaaaatg ggcagaaaaa attgccaatc
    39061 gtccagctgt taagcgtggc ttggaagtaa cttatacaga aattaaatag caaaaaagtc
    39121 atcttcggat ggctttttgc tacagttgag ggaaagggtt tgcagttatc ggcttttgtg
    39181 ctataattgc tataatatag taaaaagcaa gaggagtgtg aggaagtgga aaagaaaatt
    39241 gtgaaggata tcttattttt atctcaagtg tctcatccgg caagtcagga ggacctttat
    39301 cttgccagag atttgcagga tacactctta gcaaatcgtg atacctgtgt tggtctagct
    39361 gccaatatga ttgggatgca gaagcgcgtg attatcttta atcttggctt ggttcccgtg
    39421 gtcatgttta acccagtgct tctgtccttt gaaggatcct atgaggcaga agaaggctgt
    39481 ttgtccttgg taggtgtgag atcaactaag cgttatgaaa ccataaggct tgcctatcgt
    39541 gacagcaagt ggcaggaaca gaccattacc ttgacaggct tcccagctca gatttgccag
    39601 catgagctgg atcacttgga aggacgaatc atttaggagg aaagcaaatg aaacgaatag
    39661 tctttgaact tatttttatc gcaacgacct ggtatatctt tttaccgccc cttaacctga
    39721 ccagctggga atttctcttc ttcctctgtg ggcatttgtt agttgtggca atcttatttg
    39781 gctttggcaa ggggataaac cttgtcaaaa cggttcatgt gcgccacggt aaggcggaag
    39841 ctgccttaaa tcttgagggt ttcaaaatca atcggttagg gaaaattctg ttagcttcga
    39901 ttggaggaat tcttctcttg gcagctttgg tttccttggt aacttctagc atgtttcagg
    39961 ctaaaaatta tgccaatgta gtcacggtta cggaaaaaga ctttactgaa tttcctaaga
    40021 gtgacaccag taaggttcct atcctagata gaagtactgc tgaaaaaatt ggagaccgct
    40081 acttgggttc cctaaccgat aaggtgtcgc aatacgtagc ggcagatacc tatacccaat
    40141 tgacaattga tgggaaacct tatcgggtca caccactaga atatgcagac cctatcaaat
    40201 ggtttaacaa tcaagccaag ggaatcggtg agtatatcaa ggtggacatg gtaactggaa
    40261 atgcggattt ggtggacttg aagacaccaa tcaagtattc agactcggag tattttaacc
    40321 gtgatgtcaa acgtcacctg cgcttgaagt acccgaccaa aatctttaaa actccatctt
    40381 ttgaggtgga cgatgagggc aatcctttct atgtagcaac ggtttaccaa aagcaatttg
    40441 gacttgctgt tcctcgtcct gcttcagtca ttatcttgga tgctacaaat ggagaaacca
    40501 aggaatacag cttatcagat gttccagaat gggtggacag gatctatcca gcagaggaaa
    40561 ccattgagca aatcaactac aacggcaagt acaaggacgg tttcttgaat gccatgattt
    40621 ccaagaaaaa cgtgacccag acgaccaatg gctataatta cttgtctatc ggtaatgaca
    40681 tctatctcta cacaggtgtg acgtcggcta atgcggatga gagtaatctt ggtttcatcc
    40741 ttgaaaatat gcgaacagga gaaattacta agtatagctt ggcttctgcg acagaagaat
    40801 cagcccgtga atcagcagaa ggtgctgttc aggagaaatc ctacaaagca accttcccaa
    40861 tcctcatcaa cctcaatgac aagcctctct acatcatggg cttaaaggac aatgctggct
    40921 tggtcaaaga gtacgccctg gtagacgcag tcgagtacca aaatgttatc gttgctacta
    40981 cagtggaaga gatgctcagc aagtatgcca ataaaaacga ccttgaaatt gacaatgcaa
    41041 cgacagaaag catcaaggga gtagtagcag acctcaaatc agctgttatc aagggagaca
    41101 ctgtctactt ctttaaagtt gatggcaaga tatacaaggt aaaggcttca gtatccgatg
    41161 accttcctta ccttgaaaat ggtaaaacct tcgaaggtca agtaggaaaa gacaattatc
    41221 tcaagacctt taaagtacaa taatactctt cgaaaatctc tag
//
LOCUS       DAVBHS010000003        38545 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVBHS010000003 DAVBHS010000000
VERSION     DAVBHS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38545
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.003"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            434..886
                     /locus_tag="VQ651_000085"
     CDS             434..886
                     /locus_tag="VQ651_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2753142.1"
                     /translation="MIITKISHLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVQLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            905..2242
                     /locus_tag="VQ651_000086"
     CDS             905..2242
                     /locus_tag="VQ651_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET2753143.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2321..3205
                     /locus_tag="VQ651_000087"
     CDS             2321..3205
                     /locus_tag="VQ651_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2753144.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3205..4038
                     /locus_tag="VQ651_000088"
     CDS             3205..4038
                     /locus_tag="VQ651_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2753145.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4056..6149
                     /gene="nanB"
                     /locus_tag="VQ651_000089"
     CDS             4056..6149
                     /gene="nanB"
                     /locus_tag="VQ651_000089"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HET2753146.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKRSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNREESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6161..7264
                     /locus_tag="VQ651_000090"
     CDS             6161..7264
                     /locus_tag="VQ651_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2753147.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7438..8136
                     /locus_tag="VQ651_000091"
     CDS             7438..8136
                     /locus_tag="VQ651_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2753148.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8155..9672
                     /locus_tag="VQ651_000092"
     CDS             8155..9672
                     /locus_tag="VQ651_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2753149.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFLGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9797..11125
                     /locus_tag="VQ651_000093"
     CDS             9797..11125
                     /locus_tag="VQ651_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2753150.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11377..12243
                     /locus_tag="VQ651_000094"
     CDS             11377..12243
                     /locus_tag="VQ651_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2753151.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12259..13098
                     /locus_tag="VQ651_000095"
     CDS             12259..13098
                     /locus_tag="VQ651_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2753152.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13371..13823
                     /locus_tag="VQ651_000096"
     CDS             13371..13823
                     /locus_tag="VQ651_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2753153.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEATDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPTGYAGMEEKVRKYLFKILID"
     gene            13920..14264
                     /locus_tag="VQ651_000097"
     CDS             13920..14264
                     /locus_tag="VQ651_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753154.1"
                     /translation="MVSSKHAARASFLDGQEITVDEMSWIINGIVNALIGRYIKLGTY
                     AAKYGIGIARSILSRAAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            14548..15192
                     /locus_tag="VQ651_000098"
     CDS             14548..15192
                     /locus_tag="VQ651_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET2753155.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15361..16278
                     /locus_tag="VQ651_000099"
     CDS             15361..16278
                     /locus_tag="VQ651_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET2753156.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTATHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKESFL"
     gene            16296..17180
                     /locus_tag="VQ651_000100"
     CDS             16296..17180
                     /locus_tag="VQ651_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2753157.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17196..18047)
                     /locus_tag="VQ651_000101"
     CDS             complement(17196..18047)
                     /locus_tag="VQ651_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2753158.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANRVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18247..20304
                     /gene="pbp2b"
                     /locus_tag="VQ651_000102"
     CDS             18247..20304
                     /gene="pbp2b"
                     /locus_tag="VQ651_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HET2753159.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTKRQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEINKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20315..20911
                     /gene="recR"
                     /locus_tag="VQ651_000103"
     CDS             20315..20911
                     /gene="recR"
                     /locus_tag="VQ651_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HET2753160.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21087..22130
                     /locus_tag="VQ651_000104"
     CDS             21087..22130
                     /locus_tag="VQ651_000104"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET2753161.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22214..23587
                     /locus_tag="VQ651_000105"
     CDS             22214..23587
                     /locus_tag="VQ651_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET2753162.1"
                     /translation="MKLTIHEVAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGVRDGHDFIERAFENGAAATLSEKEVANHPYILVDDVLTAFQQLAAYYLEKTAVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGVQSVQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            23574..24185
                     /locus_tag="VQ651_000106"
     CDS             23574..24185
                     /locus_tag="VQ651_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2753163.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRDLLSEML
                     NQASDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDPLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            24276..24980
                     /locus_tag="VQ651_000107"
     CDS             24276..24980
                     /locus_tag="VQ651_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HET2753164.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25199..26572
                     /gene="ftsA"
                     /locus_tag="VQ651_000108"
     CDS             25199..26572
                     /gene="ftsA"
                     /locus_tag="VQ651_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET2753165.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENIIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVESVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26589..27848
                     /gene="ftsZ"
                     /locus_tag="VQ651_000109"
     CDS             26589..27848
                     /gene="ftsZ"
                     /locus_tag="VQ651_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET2753166.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27853..28524
                     /locus_tag="VQ651_000110"
     CDS             27853..28524
                     /locus_tag="VQ651_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET2753167.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28534..29073
                     /locus_tag="VQ651_000111"
     CDS             28534..29073
                     /locus_tag="VQ651_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HET2753168.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29073..29336
                     /locus_tag="VQ651_000112"
     CDS             29073..29336
                     /locus_tag="VQ651_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET2753169.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29333..30017
                     /locus_tag="VQ651_000113"
                     /pseudo
     CDS             29333..30017
                     /locus_tag="VQ651_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            30026..30814
                     /gene="divIVA"
                     /locus_tag="VQ651_000114"
     CDS             30026..30814
                     /gene="divIVA"
                     /locus_tag="VQ651_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HET2753170.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31162..33954
                     /locus_tag="VQ651_000115"
     CDS             31162..33954
                     /locus_tag="VQ651_000115"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET2753171.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYANFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEEPAPEAALSFEDVVFTVERAAGEVCDRCRRIDPTTAERSYHAVICDHCASI
                     VEENFADAVAEGFEEK"
     gene            34364..34726
                     /locus_tag="VQ651_000116"
     CDS             34364..34726
                     /locus_tag="VQ651_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753172.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNALKIANLVLGIIAIVYYKGDKHVGAAPSVLMIVSGEVSLIPFLGWVGGILA
                     IIGGSLFLATLKKFKSEE"
     gene            35261..35953
                     /locus_tag="VQ651_000117"
     CDS             35261..35953
                     /locus_tag="VQ651_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HET2753173.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            36006..38417
                     /locus_tag="VQ651_000118"
     CDS             36006..38417
                     /locus_tag="VQ651_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET2753174.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     RKAFASFPDDLLIQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYSGNLFSY
                     KGQEYIEAARAILNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
BASE COUNT        11842 a         6802 c         8415 g        11486 t
ORIGIN      
        1 ttagaaaaac gaagtgagca gcttataaat tcaagtcccc aaatagattc atactagtat
       61 cttttgcaaa aaataaaggg cgacttcctt catgaatatc aatttcatct ataaggaagg
      121 tagctaattg aactaactta tttattctgt ttgtcgctag aaaaatcaga cctccttgtg
      181 aagattgagg agatactgaa tgaaaatcaa agaagaaact agcaagctag tagcagattg
      241 cccaaaacac cgccttgagg ttgtagataa gactgaccta tataatccaa ggtgaagcga
      301 ctgtggtttg aagagatttt caaagagtat aggctagaga gtagtgtttt tatgtccttc
      361 tagtagaaaa tgctagacag aagaatgggg aacttggata ggaaaaatag attgagaaag
      421 gaggttagaa gagatgatta ttacaaaaat tagccattta ggaacttatg tgggagtaaa
      481 tccacatttt gcaacattaa tagattttct agaaaaaaca ggactagaaa atttaacaga
      541 aggttcgatt gctatcgatg gtaatcgatt gtttgggaat tgctttactt atctagcaga
      601 tggtcaagca ggggctttct ttgaaacaca ccaaaaatat ttggatattc atttagtttt
      661 ggaaaacgaa gaagccatgg ctgttacatc gccggaaaat gtaagcgtta cccaagaata
      721 tgatgaagag aaagatattg aattatacac agggaaagtg gaacagttgg ttcagttgag
      781 agctggcgaa tgcctcatca cttttccaga agatttacat caacccaagg ttcgtataaa
      841 tgatgaacct gtgaaaaaag ttgtctttaa agttgcgatt tcttaatgta gaaagagaag
      901 aacgatgaaa aaaatgagaa agtttttatg tctagctgga attgcgctag cggctgttgc
      961 cttggtagct tgttcaggaa aaaaagaagc tacaactagt actgaaccac caacagaatt
     1021 atctggtgag attacaatgt ggcactcctt tactcaagga ccccgtttag aaagtattca
     1081 aaaatcagca gatgctttca tgcaaaagca tccaaaaacg aaaatcaaga ttgaaacatt
     1141 ttcttggaat gacttctata ctaaatggac tacaggttta gcaaatggaa atgtgccaga
     1201 tatcagtaca gctcttccta accaagtaat ggaaatggtc aactcagatg ctttggttcc
     1261 gctaaatgat tctatcaagc gtattggaca agataaattt aacgaaactg ccttaaatga
     1321 agcaaaaatc ggagatgatt actactctgt tcctctttat tcacatgcac aagtcatgtg
     1381 ggttagaaca gatttgttaa aagaacataa tattgaggtt cctaaaactt gggatcaact
     1441 ctatgaagct tctaaaaaat tgaaagaagc tggagtttat ggcttgtctg ttccgtttgg
     1501 aacaaatgac ttaatggcaa cacgtttctt gaacttctac gtacgtagtg gtggaggaag
     1561 cctcttaaca aaagatctta aagcagactt gacaagccaa cttgctcaag atggtattaa
     1621 atactgggtt aaattgtata aagaaatctc acctcaagat tctttgaact ttaatgtcct
     1681 tcaacaagct accttgttct atcaaggaaa aacagcattt gactttaact ctggcttcca
     1741 tatcggagga attaatgcca acagtcctca attgattgat tcgattgatg cttatcctat
     1801 tccaaaaatc aaagagtctg ataaagacca aggaattgaa acctcaaaca ttccaatggt
     1861 tgtttggaaa aattcaaaac atccagaagt tgctaaagca ttcttagaag cactttataa
     1921 tgaagaagac tacgttaaat tccttgattc aactccagta ggtatgttgc caactattaa
     1981 ggggattagc gattctgcag cctataaaga aaatgaaact cgtaagaagt ttaaacatgc
     2041 tgaagaagta attactgaag ctgttaaaaa aggtactgct attggttatg aaaatggacc
     2101 aagtgtacaa gctggtatgt tgactaacca acacattatt gaacaaatgt tccaagatat
     2161 cattacaaat ggaacagatc ctatgaaagc agcaaaagaa gcagaaaaac aattaaacga
     2221 tttatttgag gctgttcagt agatgtaaaa gactagaaaa taggtgggct agtgagctga
     2281 aaagctctag cccaatcttg taaaagaagg gagaaggaga atggttaaag aacgtaattt
     2341 aactcgctgg atatttgttt tgccagctat gattatcgta ggattactct ttgtttatcc
     2401 gtttttctcg agtatttttt atagctttac caataagcat ttgattatgc ctaattataa
     2461 atttgttggt ttggctaact ataaagctgt gctatcagat cccaacttct ttaatgcgtt
     2521 ctttaattca attaagtgga ccgttttctc attagttggt caagttttag tagggtttgt
     2581 attggcttta gctcttcaca gagtacgcca cttcaagaaa ttatatagga ccttattgat
     2641 tgttccttgg gcatttccta ccatcgttat tgccttttct tggcagtgga ttctaaacgg
     2701 ggtttatggc tacttaccta atctaatcgt aaaattaggt ttaatggaac atacacctgc
     2761 atttttgaca gatagtacat gggcattcct atgtttggtg tttatcaaca tttggtttgg
     2821 agcaccaatg attatggtta atgtgctttc agctttgcaa acagtaccag aagaacaatt
     2881 tgaggctgct aagatagatg gtgcttcaag ttggcaggtg ttcaagttta tcgtctttcc
     2941 acatattaaa gtggttgtag gacttctagt tgttttgaga actgtatgga tctttaataa
     3001 ctttgacatt atctacctca ttactggtgg tggaccagcc aatgctacaa cgacgcttcc
     3061 aatttttgct tacaacctgg gctggggaac taaattgttg ggtcgtgctt cagcagttac
     3121 agtattgctc tttatcttct tggtggcgat ttgctttatc tactttgcta tcatcagtaa
     3181 gtgggaaaag gagggtagaa aataatgaag aagaaatcca gtatttattt agatattctc
     3241 tcacatgtac ttttagttgg tgcgaccatc gttgcaattt tcccattggt atggattatc
     3301 atatcttctg tcaaagggaa aggggaatta actcagtatc caacacgatt ttggcctgaa
     3361 cagtttacat tagattattt cactcatgtt atcaacgatt tgcacttcat tgataacatt
     3421 cgaaacagtt taatcattgc cttggctaca accctcattg cgattattat ttctgctatg
     3481 gcagcctatg gtattgttcg attctttcct aaattgggag caatcatgtc gagactactc
     3541 gtcattacct acattttccc accaattttg ttagcaattc cctattcaat tgccattgct
     3601 aaagttgggt taacaaatag tttatttggc ttgatgatgg tttatctatc ttttagtgtt
     3661 ccatatgcag tttggctctt agttggattt ttccaaacag ttccaattgg aattgaagaa
     3721 gcggctagaa ttgatggtgc aaataaattt gttacgtttt ataaagttgt gctaccgatt
     3781 gtagcaccag gtattgtagc aacagctatt tatacattta tcaatgcttg gaatgaattc
     3841 ctgtatgcct tgattttgat taacaataca ggaaagatga cagtagcagt agcccttcgt
     3901 tcacttaatg gttcagaaat actagactgg ggagatatga tggcagcgtc tgttattgta
     3961 gttcttccat caattatttt cttctctatc atccaaaata agattgcaag tggattatca
     4021 gaaggatctg tgaagtagac gaaagaagga aaaaaatgaa taaaagaggt ctttattcaa
     4081 aactaggaat ttctgttgta ggcattagtc ttttaatggg agtccccact ttgattcatg
     4141 cgaatgaatt aaactatggt caactgtcca tatctcctat ttttcaagga ggctcatatc
     4201 aactgaacaa taagagtata gatatcagcc ctttgttatt agataaattg tctggagaga
     4261 gtcagacagt agtaatgaaa tttaaagcag atgaaccaaa ctctcttcaa gctttgtttg
     4321 gcctatctaa tagtaaagca ggctttaaaa ataattactt ttcaattttc atgagagatt
     4381 ctggtgagat aggtgtagaa ataagagacg cccaaaaggg aataaattat ttattttcca
     4441 gaccagcttc attatgggga aaacataaag gacaggcagt tgaaaataca ctagtatttg
     4501 tatctgattc taaagataaa acatacacaa tgtatgttaa tggaatagaa gtgttctctg
     4561 aaacagttga tacatttttg ccaatttcaa atataaatgg tatagataag gcaacactag
     4621 gagctgttaa tcgtgaaggc aaggaacatt acctcgcaaa aagaagtatt gatgaaatca
     4681 gtctatttaa caaagcaatt agtgatcagg aagtttcaaa tattcccttg tcaaatccat
     4741 ttcagttaat tttccaatca ggagattcta ctcaagctaa ctattttaga ataccgacac
     4801 tatatacatt aagtagtgga agagttctat caagtattga tgcacgttat ggtgggactc
     4861 atgattctaa aagtaagatt aatattgcca cttcttatag tgatgataat gggaaaacgt
     4921 ggagtgagcc aatttttgct atgaagttta atgactatga ggagcagtta gtttactggc
     4981 cacgagataa taaattaaag aatagtcaaa ttagtggaag tgcttcattc atagattcat
     5041 ccattgttga agataaaaaa tctgggaaaa cgatattact agctgatgtt atgcctgcgg
     5101 gtattggaaa taataatgca aataaagccg actcaggttt taaagaaata aatggtcatt
     5161 attatttaaa actaaagaag aatggagata acgatttccg ttatacagtt agagaaaatg
     5221 gtgtcgttta tgatgaaaca actaataaac ctacaaatta tactataaat gataagtatg
     5281 aagttttgga gggaggaaag tctttaacag tcgaacaata ttcggttgat tttgatagtg
     5341 gctctttaag agaaaggcat aatggaaaac aggttcctat gaatgttttc tacaaagatt
     5401 cgttatttaa agtgactcct actaattata tagcaatgac aactagtcag aatagagaag
     5461 agagttggga acaatttaag ttgttgcctc cgttcttagg agaaaaacat aatggaactt
     5521 acttgtgtcc tggacaaggt ttagcattaa aatcaagtaa cagattgatt tttgcaacat
     5581 atactagtgg agaactaacc tatctcattt ctgatgatag tggtcaaaca tggaagaaat
     5641 cctcagcttc aattccgttt aaaaatgcaa cagcagaagc acaaatggtt gaactgagag
     5701 atggtgtgat tagaacattc tttagaacca ctacaggtaa gatagcttat atgactagta
     5761 gagattctgg agaaacatgg tcggaagttt cgtatattga tggaatccaa caaacttcat
     5821 atggcacaca agtatctgca attaaatact ctcaattaat tgatggaaaa gaagcagtca
     5881 ttttgagtac accaaattct agaagtggcc gtaagggagg tcaattagtt gtcggtttag
     5941 tcaataaaga agatgatagt attgattgga aataccacta tgatattgat ttgccttcgt
     6001 atggttatgc ctattctgcg attacagaat tgccaaatca tcacataggt gtactgtttg
     6061 aaaaatatga ttcgtggtcg agaaatgaat tgcatttaag caatgtagtt cagtatatag
     6121 atttggaaat taatgattta acaaaataaa ggagaaaaac atggttaaat acggtgttgt
     6181 tggagcaggg tattttggag ctgaattggc tcgctacatg caaaagaatg atggagcaga
     6241 gattactctt ctctatgatc cagataatgc agaggcgatt gcagaagaat tgggagcaaa
     6301 agtagcaagt tccttagatg agttggtttc tagcgatgaa gtagattgtg ttatcgtcgc
     6361 aactccaaat aatcttcata aggaaccggt tattaaggct gcacagcatg gtaaaaatgt
     6421 tttctgtgaa aaaccaattg cgctttctta tcaagattgt cgcgagatgg tagatgcgtg
     6481 taaagaaaac aatgtaacct ttatggcagg acatattatg aatttcttta atggtgttca
     6541 tcatgcaaaa gaactcatta atcaaggagt tatcggagac gttctatatt gtcatacagc
     6601 tcgtaatggt tgggaagaac aacaaccgtc agtgtcatgg aaaaaaattc gtgaaaaatc
     6661 aggtggtcac ttgtatcacc acatccatga attggattgc gttcaattcc ttatgggggg
     6721 catgcctgaa actgtaacca tgacaggtgg aaatgtggcc catgaaggtg aacatttcgg
     6781 tgatgaagat gatatgattt ttgtcaatat ggaattttct aataagcgtt ttgccttgtt
     6841 agaatggggt tcagcttatc gttggggtga acattatgtc ttaatccaag gaagcaaagg
     6901 tgccatccgc ttagacttat tcaactgtaa aggaactctt aagctagatg ggcaagaaag
     6961 ctatttcttg attcacgaat cgcaagaaga agatgatgat cggactcgta tctatcatag
     7021 tacagagatg gatggagcaa ttgcttatgg taaaccaggt aaacgtactc cattatggct
     7081 atcatctgtc attgataaag aaatgcgcta tctgcatgag attatggaag gagctccagt
     7141 atcagaagaa tttgcaaaac ttttgacagg tgaagctgcc ctagaagcaa ttgctactgc
     7201 agatgcttgt acccagtcta tgtttgaaga tcgcaaagta aaattgtcag aaattgtaaa
     7261 ataaattttg gtattctcct atttataggt cgacttgctc ctctgaaagt acttttagag
     7321 gagctgtttg actttgctag tttttgaaac tgaaatctat tatactacaa actattgaaa
     7381 gcgttttaat tttaaggtat aataatctca tagaaataaa gaaaaggagg aaagaggatg
     7441 ccacagatta gcaaagaagc cttgattgag caaatcaaag atggaatcat cgtttcttgt
     7501 caggctcttc ctcatgaacc gctttataca gaagcgggag gggtgattcc cttgctggtc
     7561 aaagcggctg agcaaggtgg agcagtcggt atccgagcaa acagtgttcg cgatatcaag
     7621 gaaattaagg aagtcactaa acttccaatc attgggatta tcaaacgtga ttatccacct
     7681 caggaaccct tcatcacggc tactatgaaa gaagttgatg aattggcaga actggacatc
     7741 gaggtgattg ctctggattg taccaagcgt gaacgctacg atggtttgga aattcaagag
     7801 ttcattcgtc aggttaagga gaaatatcct aatcagcttt tgatggctga tactagtatc
     7861 ttcgaagaag ggctagcagc tgtagaagca ggaattgact ttgtcggaac aaccttatca
     7921 ggctacacat cctacagtcc aaaagtagac ggtccagatt ttgaattgat taagaaactc
     7981 tgtgatgccg gtgtagatgt cattgcagaa ggaaaaattc atacaccaga acaagccaaa
     8041 caaatccttg aatatggagt gcgaggcatc gttgttggtg gcgccattac tagaccaaaa
     8101 gagattacag aacgcttcgt tgctagtctt aaataagatg tgagggggag ttttatgttt
     8161 aaagttttac aaaaagttgg aaaagctttt atgttaccta tagctatact tcctgcagca
     8221 ggtctacttt tggggattgg tggtgcactt tcaaacccaa ccacgatagc aacttatcca
     8281 atactagaca atagtatttt tcaatcaata ttccaagtaa tgagctctgc aggagaggtt
     8341 gtattcagta atttgtcact acttctctgt gtgggattat gtattggctt agcgaaacga
     8401 gataaaggaa ccgctgcgtt agcaggagta actggttact tagttatgac tgcaacgatc
     8461 aaagctttag taaaactttt tatggcagaa ggatctgcaa ttgatactgg agttattgga
     8521 gcattagttg tcggaatagt tgccgtatat ttgcacaacc gatataacaa tattcaatta
     8581 ccttccgctt taggattctt tggaggttca cgcttcgttc ctattgttac atcgttctct
     8641 tctatcttga ttggctttgt cttctttgtt atttggccac ctttccaaca acttcttgtt
     8701 tctacaggtg gatatatttc tcaggcgggt ccaattggaa cttttctata tggattttta
     8761 atgagacttt ctggagcagt aggcttacat catataattt accctatgtt ttggtatact
     8821 gaacttggtg gtgttgaaac tgttgcagga caaacagtgg ttggagctca aaaaatattt
     8881 tttgctcaat tagccgattt ggcccattct ggattattta cagaaggaac aaggtttttt
     8941 gcaggtcgtt tctcaacaat gatgttcggt ttaccggctg cctgtttagc gatgtaccat
     9001 agtgttccta aaaatcgtcg taaaaaatac gcgggtttgt ttttgggagt tgctttaaca
     9061 tcttttatta ccggtattac agaaccaatt gaatttatgt ttctattcgt tagtccggtt
     9121 ctatatgttg ttcacgcatt ccttgatggt gttagcttct ttattgcaga cgtcttaaat
     9181 atttcaatag gaaacacatt ttcaggaggt gtaatcgatt tcactttatt tggaattttg
     9241 caggggaacg ctaagacgaa ttgggttctt cagattccat ttggacttat ttggagtgtt
     9301 ttgtattata ttatttttag atggttcatt actcaattca acgttctaac gccagggcga
     9361 ggagaagaag tagattctaa agaaatttct gaatccgcag attcaacttc aaatactgca
     9421 gattatttaa aacaggatag cctacaaatt atcagagcct tgggtggatc aaataatata
     9481 gaagatgtag atgcttgtgt gacacgttta cgtgtagctg taaaagaagt taatcaagtt
     9541 gataaagcac ttttaaaaca aattggtgca gttgatgtct tagaagtgaa gggtggcatt
     9601 caagcaatat atggagcaaa agcaatctta tataaaaata gtattaatga aattttaggt
     9661 gtagatgatt aagtacttac tgacttaata aaaaacagag gagagtgatg gatgagtagg
     9721 atgaaatgaa atcgcataca agaaataaag aactcattat ccaagttgga tacgcttatt
     9781 acataggaga atacaaatga aatttagaaa attagcttgt acagtacttg cgggtgctgc
     9841 ggttcttggt cttgctgctt gtggcaattc tggcggaagt aaagatgctg ccaaatcagg
     9901 tggtgacggt gccaaaacag aaatcacttg gtgggcattc ccagtattta cccaagaaaa
     9961 aactggtgac ggtgttggaa cttatgaaaa atcaatcatc gaagcgtttg aaaaagcaaa
    10021 cccagatata aaagtgaaat tggaaaccat cgacttcaag tcaggtcctg aaaaaatcac
    10081 aacagccatc gaagcaggaa cagctccaga cgtactcttt gatgcaccag gacgtatcat
    10141 ccaatacggt aaaaacggta aattggctga gttgaacgac ctcttcacag atgaatttgt
    10201 taaagatgtc aacaatgaaa acatcgtaca agcaagtaaa gctggagaca aggcttatat
    10261 gtatccgatt agttctgccc cattctacat ggcaatgaac aagaaaatgt tagaagatgc
    10321 tggagtagca aaccttgtaa aagaaggttg gacaactgat gattttgaaa aagtattgaa
    10381 agcacttaaa gacaagggtt acacaccagg ttcattgttc agttctggtc aagggggaga
    10441 ccaaggaaca cgtgccttta tctctaacct ttatagcggt tctgtaacag atgaaaaagt
    10501 tagcaaatat acaactgatg atcctaaatt cgtcaaaggt cttgaaaaag caactagctg
    10561 gattaaagac aatttgatca ataatggttc acaatttgac ggtggggcag atatccaaaa
    10621 ctttgccaac ggtcaaacat cttacacaat cctttgggca ccagctcaaa atggtatcca
    10681 agctaaactt ttagaagcaa gtaaggtaga agtggtagaa gtaccattcc catcagacga
    10741 aggtaagcca gctcttgaat accttgtaaa cggatttgca gtattcaaca ataaagacga
    10801 caagaaagtc gctgcatcta agaaattcat ccagtttatc gcagatgaca aagagtgggg
    10861 acctaaagac gtagttcgta caggtgcttt cccagtccgt acttcatttg gaaaacttta
    10921 tgaagacaaa cgcatggaaa caatcagcgg ctggactcaa tactactcac catactacaa
    10981 cactattgat ggttttgctg aaatgagaac actttggttc ccaatgttgc aatctgtatc
    11041 aaatggtgac gaaaaaccag cggatgcttt gaaagccttc actgaaaaag caaacgaaac
    11101 aatcaaaaaa gctatgaaac aatagtcctt agttattcta taaaaagtag ttttttaaag
    11161 aacctaagag tgtatacccc cttttccctc tacacagata gtgtaagaaa agggggcttt
    11221 tgtttaaaat gtaagaaact gtcacgaaat taaaatgaag ttcttacata agcgaatcat
    11281 aaaaaatttc attttgattt taaaacagtt caagaaagtc aaaaaattat tctatttgaa
    11341 agagaggtgc cgactgtgaa agtcaataaa atccgtatgc gggaaacagt gatttcctac
    11401 gctttcctag caccagtatt attcttcttt gtcatctttg tgttggctcc gatggtgatg
    11461 ggcttcatta caagtttctt taactactca atgactaaat ttgagtttgt aggcttggat
    11521 aactatatcc gtatgtttaa agatcctgtc tttacaaaat ctctgattaa cacagttatt
    11581 ttggttattg gatctgtacc agttgttgtt ctattctcac tctttgtagc atctcagacc
    11641 tatcatcaaa atgtcattgc cagatccttc taccgtttcg tcttcttcct tcctgttgta
    11701 acgggtagtg ttgccgtgac agttgtttgg aaatggattt atgacccact atcagggatt
    11761 ctaaactttg tccttaagtc cagccacatc atcagccaaa acatttcttg gttgggagat
    11821 aaaaactggg cattgatggc gattatgatt attctcttga ccacttcagt tggtcagccc
    11881 atcatccttt atatcgctgc catggggaat attgacaatt cactggttga agcggcgcgt
    11941 gttgatggtg caactgagtt tcaagttttt tggaagatta aatggccaag ccttcttcca
    12001 acaactcttt atattgcaat catcacaaca attaactcat tccagtgttt cgccttgatt
    12061 cagcttttga catctggtgg tccaaactac tcaacaagta ccttgatgta ctacctttac
    12121 gaaaaagcct tccaattgac agaatacggc tatgccaaca caattggtgt cttcttggca
    12181 gtcatgattg ctatcgtaag ctttgttcaa tttaaagtac ttggaaacga cgtagaatac
    12241 taaagaaagg agacagctat gcaatctaca gaaaaaaaac cattaacagc ctttactgtt
    12301 atttcaacaa tcattttgct cttgttgact gtgctgttca tctttccatt ctactggatt
    12361 ttgacagggg cattcaaatc acaacctgat acaattgtta ttcctcctca gtggttccct
    12421 aaaatgccaa ccatggaaaa cttccaacaa ctcatggtgc agaaccctgc cttgcaatgg
    12481 atgtggaact cagtatttat ctcattggta accatgttct tagtttgtgc aacctcatct
    12541 ctagcaggtt atgtattggc taaaaaacgt ttctatggtc aacgcattct atttgctatc
    12601 tttatcgctg ctatggcgct tccaaaacaa gttgtccttg taccattggt acgtatcgtc
    12661 aacttcatgg gaatccacga tactctctgg gcagttatct tgcctttgat tggatggcca
    12721 ttcggtgtct tcctcatgaa acaatttagt gaaaatattc ctacagagtt gcttgaatca
    12781 gctaaaatcg acggttgtgg tgagattcgt accttctgga gtgtagcctt cccgattgtg
    12841 aaaccagggt ttgcagccct tgcaatcttt accttcatca atacttggaa tgactacttc
    12901 atgcagttgg taatgttgac ttcacgtaac aatttgacca tctcacttgg ggttgcgacc
    12961 atgcaggctg aaatggcaac caactatggt ttgattatgg caggagctgc ccttgctgct
    13021 gttccaatcg tcacagtctt ccttgtcttc caaaaatcct tcacacaggg tattactatg
    13081 ggagcggtca aaggataata ctctgcgaaa atcgaatgca aactacgtca gcttcacctt
    13141 gccatactta agtattgcct gcggttagct tcctagtttg ttcttcaatt ttcattgagt
    13201 ataggaaaat caatctatca agatacagaa gtatatttta tagatttaga gaatatagaa
    13261 gttataagtg tctacaaaat ggagggtatg cagttacttt atgaagtttt gtcagacact
    13321 tatcaactta agaatgattt tagttaatta tcagaaacga aggaaagagt atgatttttg
    13381 acgatttgaa aaacatcacc ttttacaaag ggattcatcc caatttagac aaagctatcg
    13441 actacctcta ccaacatcgt aaagattcat tcgaattagg aaagtatgag attgatggag
    13501 ataaggtctt tctagttgtt caggaaaatg tcctcaatca agttgagaat aatcaatttg
    13561 aacaccataa gaactatgca gatttgcatt tgctgataga agggcatgaa tattcgagct
    13621 acggttcacg tatcaaagac gaggcagtag cattcgacga agcgactgac attggctttg
    13681 ttcattgtca tgaacactac ccactcttgt tgggttatca taattttgcg attttcttcc
    13741 caggtgagcc acatcagcca actggctatg caggtatgga agaaaaggtt cgaaaatatc
    13801 tctttaaaat tttgattgat taaaaatagg atgaattgtt tttttgtaaa gctttgacaa
    13861 tactctacca tgaaattgat ctttatgagg tagagaaatg agaataaaat atttaaaaat
    13921 tggtatcttc taagcatgct gcaagagcta gtttcttaga tggacaggaa attacagttg
    13981 atgagatgtc ctggataatt aatggcattg tgaatgcatt gattggtaga tacataaaat
    14041 taggtactta tgcagctaag tatggtattg gtatagcacg ctcgatctta agtagggcag
    14101 ctgcaactgc agcagcaaga gtaggattac tgaccaagat ttctggatgg attttacgag
    14161 tagctgtgaa tgtagctgat gtatatggta atttcgccaa caatattgct gcagcttggg
    14221 atgcatatga taaaattcct aacaatggtc gtataaactt ttaaaatacg agaatgaaag
    14281 cactttgtat ttttttattg aatatgttag cttggacagt gcttgcaatg ataattcgtg
    14341 gagggctaga tggatttgat aggcatactt ggagtactat tttaattgct tcgctgttcg
    14401 gggtatatga ttataagccc atagataaaa atagaaaaaa gtccaaaaga aaaaagaaaa
    14461 aatagatttg ttcatggtag ggacttatga aagctttact gacaaaaaag aaaacagttt
    14521 acaaagaaaa atgatggagg agcaaacatg gcacaaaaag gagtaagcct tatcaaggca
    14581 gcatttgata cagataactt tctcatgcgt tttagtgaga aggtcttgga catcgtgaca
    14641 gccaatcttc tttttgtcgt ctcttgttta cccatcgtga cgattggagt ggctaaaatc
    14701 agcctctacg agaccatgtt cgaagttaag aagagcagac gggtgcctgt ttttaaaatc
    14761 tatctaagat ctttcaagca aaatctgaaa ctaggtcttc agctgggttt aatggagtta
    14821 ggaattgtgt ttcttaccct ttcagatctc tatcttttct ggggtcaaac agctctgccc
    14881 ttccaattgc tgaaagccat ttgtttaggt attctgattt ttcttactat cgtgatgctg
    14941 gctagttacc ctatcgcggc acgttatgac ctatcttgga aagaaattct tcaaaaagga
    15001 ttgatgttgg ctagttttaa ctttccttgg ttcttcctca tgttagccat tcttgtcctc
    15061 attgtgatgg ttctttatct gtccgccttc agtctactct taggtggctc agtcttccta
    15121 ctttttgggt ttggactatt ggtctttatc cagactggat tgatggagaa aattttcgca
    15181 aaataccaat aggagcttta tttctgaaac tactttcaaa ggctccaaac gctattctat
    15241 aagcgagaaa ctaaaatcta agtgtctata agatattgaa agccttttcg gtaaagtgta
    15301 tactaaagaa gtaaagaaaa atctgcttta gcagaaaata aaaataatag gagaaaatct
    15361 atgtcagatt tgaaaaaata cgaaggtgtc attccagcct tctacgcatg ttatgatgag
    15421 caaggagaag taagtccaga gcgtacgcgt gccttggttc aatacttcat tgataaaggt
    15481 gttcaaggtc tttatgtcaa tggttcttct ggtgaatgta tctaccaaag cgttgaagat
    15541 cgcaagttga ttttggaaga agtcatggcg gtagctaaag gtaaattgac cattattgcc
    15601 catgttgcct gcaacaatac taaagacagt atggaacttg ctcgccatgc tgaaagcttg
    15661 ggagtagatg ctattgcaac gattccacca atttatttcc gcttgccaga atactcagtt
    15721 gccaaatact ggaacgatat cagttctgca gctccaaaca cagactacgt gatttacaac
    15781 attcctcaat tggcaggggt tgctttgact ccaagccttt acacagaaat gttgaaaaat
    15841 cctcgtgtta tcggtgtgaa gaactcttct atgccagttc aagatatcca aacctttgtc
    15901 agccttggtg gggaagacca tatcgtcttt aacggtccag atgaacagtt cctaggtggt
    15961 cgcctcatgg gtgccaaagc tggtatcggt ggtacttatg gtgctatgcc agaactcttc
    16021 ttgaaactca accaattgat tgcggataaa gatttagaaa cagcgcgtga gttacagtat
    16081 gctatcaacg ctatcattgg taaattgact gctacacatg gaaatatgta cggtgtcatc
    16141 aaagaagttt tgaagatcaa tgaaggcttg aatattggtt ctgttcgttc accattgaca
    16201 ccagtgactg aagaagatcg tccagttgta gaagcggctg ctgccttgat tcgtgaaacc
    16261 aaggagagct tcctctaatc taaaaggagg tatttatgac atactacgtt gcaattgata
    16321 tcggtggaac caacatcaag tatggtttgg ttgatcaaga ggggcaactt cttgaatcgc
    16381 atgaaatgcc aactgaggcg cataagggtg gacctcatat cttacaaaag accaaagata
    16441 tcgtagctag ttatttagaa aaaggcccag tagcaggtgt tgccatatct tctgctggga
    16501 tggtggatcc ggataagggt gagattttct atgctgggcc gcaaatccct aactacgcag
    16561 gcacccagtt caaaaaggaa atcgaagaaa gctttactat tccttgtgag attgaaaatg
    16621 atgtcaactg tgcaggtctt gctgaggcag tatctggttc aggcaaggga gcaagtgtga
    16681 cactttgctt gaccattgga accggtatcg gtggttgctt gattatggat aggaaagtct
    16741 tccatggttt tagcaattca gcctgtgaag tcgggtatat gcatatgcag gatggagctt
    16801 ttcaagactt ggcttctaca acagctttag tgaaatatgt agctgaagcc catggagaag
    16861 atgttgatca gtggaatggc cgtagaattt tcaaagaagc cactgaagga aacaaaatct
    16921 gcatggaagg tattgaccgt atggttgact atctaggaaa aggtctggca aatatttgct
    16981 acgttgccaa tccagaagtg gttattcttg gtggtggtat catggggcaa gaggctatcc
    17041 tcaaacctaa gatccgcaca gccttgaaag aggctttggt accaagttta gcagaaaaaa
    17101 cacgattaga atttgcccat caccaaaata cagcagggat gttgggtgca tattatcatt
    17161 ttaagacaaa acaatcctag tttggctcag ccaaactagg attttcttac acgtttttgt
    17221 ctacgatagc cgttgagttt tttattttcc cagtagctat taaagatttt ttccttgctt
    17281 tcgcgattga tttccaaaaa gtaggcataa atcaaatcga taaagaagag cataggaagt
    17341 tgagcggata ttcgttggat ataggagggt tggctgtggg tggctacaag aacagtctct
    17401 gtataggtct ggctatcttt attgggaaca cttgtaaaga gtacagtctt tgcccccatc
    17461 tccttggcat ctaatagact atctaaaata gaaggagttg agcctgaaag tgagaagccc
    17521 agtactagac aattttcatc catgatgctg gttgtccagg caaagccgtc ttggtctgtc
    17581 aaagcttcgc agaccacacc tagtcgcata aaacgtaatt tcatttcacg ggcgacgagg
    17641 ccagaactcc ctgttccaaa gaagtagata cgctcagcat cttcgattag ctgggcaatt
    17701 cgctctagtt ggatttcgtc aatcaagtct tgtgtttgtt ccctcatatt gctataactt
    17761 ctgaggactc gtttggtcag tggactgtgc ttggagactc ggttggcttg attttctgcc
    17821 tgatgttggt attggaaaat aaattctcgg tagccagtaa agccacactt tttagcaaag
    17881 cgggtcaaag cagcttgaga aatatgtaat ttttgggtga cttgttgaga agataaatca
    17941 tctgtaatcg tttcagcttg caaaaaatag cgagcgattt cttgctctag gtctgtcatt
    18001 tcttcaaaat gtgaatcaat gatagttgcg atatctggtt tgtccatagg gaaagctcct
    18061 ttacatgagt catactggaa gactagatca gggaatagtc acacttcatt ataacacata
    18121 atataaggat agataaataa aaacgcatct ctgttttaaa aacgaaaaaa tcgaaaaagc
    18181 ttctctcttt tccataattt tctactcaaa ttgtggtaca attaagagta agattttaag
    18241 ttagaaatga gactgatttg tatgagaaaa tttaacagcc attcgattcc gattcggctt
    18301 aatttattgt tttcaatcgt cattttactc tttatgacca ttattggtcg tttgttgtat
    18361 atgcaggttt tgaacaagga tttttacgaa aaaaagctag cctcagctag tcagaccaag
    18421 attacaagca gttcagcccg tggggaaatt tatgatgcta gtggaaaacc tttggtagaa
    18481 aatacgttaa agcaggttgt ttcctttacg cgtagcaata aaatgacggc tacagactta
    18541 aaagaaacag ctaaaaagtt actgacttat gtgagcatca gttctccaaa tttgacaaaa
    18601 cgccagctgg cggattacta tttggctgat cctgaaatct ataaaaaaac agtggaagct
    18661 ctcccaagcg agaaacgctt ggattcagat ggcaatcgtc tatccgaatc agaactgtat
    18721 aacaatgcgg tcgatagtgt ccaaacaagt caactaaact atacagagga tgaaaagaaa
    18781 gaaatctatc tttttagtca gttaaatgct gttggaaact ttgcgacagg aaccattgct
    18841 acagatcctc taaatgattc tcaggtggct gttattgcct ctatttcaaa ggagatgcct
    18901 ggcattagta tttctacttc ttgggataga aaggttttgg aaacttccct ttcttctata
    18961 gttgggagtg tatccagtga aaaagctggt ctcccagcgg aagaagcaga agcctatctt
    19021 aaaaaaggct attctctaaa tgaccgtgta ggaacctcct atttggaaaa gcaatatgaa
    19081 gagaccttac aaggaaaacg atcggtaaaa gaaatccatc tggataaata tggcaatatg
    19141 gaaagcgtgg atacaattga ggaaggtagt aagggaaaca atatcaaact gaccattgat
    19201 ttggccttcc aagatagcgt ggatgctttg ctgaaaagtt atttcaattc tgagctagaa
    19261 aatggtggag ccaagtattc tgaaggtgtc tatgcagtcg cccttaaccc aaaaacaggt
    19321 gcggttttgt ctatgtcagg gattaaacat gacttgaaaa cgggagagtt gacgcctgat
    19381 tccttgggaa cggtaaccaa tgtctttgtt ccaggttcgg ttgtcaaggc ggcgaccatc
    19441 agctcaggtt gggaaaatgg agtcttgtca ggaaaccaga ccttgacaga ccagtccatt
    19501 gtcttccaag gttcagctcc catcaattct tggtatactc aggcttacgg ttcattccct
    19561 atcacagcgg tccaagctct ggagtattca tcaaatacct atatggtcca aacagcctta
    19621 ggtcttatgg ggcaaaccta tcaacccaat atgtttgtcg gcaccagcaa tctagagtct
    19681 gctatggaga aactgcgttc aacctttggc gaatatggct tgggtactgc gacaggaatt
    19741 gacctaccag atgaatctac tggatttgtt cccaaagagt atagctttgc taattacatt
    19801 actaatgcct ttgggcagtt tgataactat acgccgatgc agttggctca gtatgtagca
    19861 actattgcaa ataatggtgt tcgtgtggct cctcgtattg ttgaaggcat ttatggtaat
    19921 aatgataagg gaggactggg tgacttgatt cagcaactgc aaccgacaga gataaataag
    19981 gtcaatatat ccgactccga tatgagcatc ttgcaccaag gtttttatca ggttgcccat
    20041 ggtactagtg gattgacaac tggacgtgcc ttttcaaatg gtgccttggt atccattagc
    20101 ggaaaaacag gtacagccga aagctatgtg gcagatggtc agcaagcaac caataccaat
    20161 gcggtggcct atgccccatc tgataatccc caaatcgctg tcgcagtggt ctttcctcat
    20221 aataccaatc taacaaatgg tgtaggacct tccattgcgc gtgacattat caatctgtat
    20281 caaaaatacc atccaatgaa ctagaaagga aattatgctt tatccaacac ctattgccaa
    20341 gttgattgac agttattcta agttaccagg tatcgggatt aagacggcta cgcgtctggc
    20401 cttttatacg attgggatgt ctgctgatga tgtcaatgaa tttgcaaaaa atctcctttc
    20461 tgctaagaga gaattgacat attgttctat ttgtggacgt ttgacagacg acgatccttg
    20521 ttctatctgt actgatccga ctcgtgacca gacaacaatt ttagttcttg aggatagtag
    20581 agatgtggca gccatggaaa atatccaaga ataccatgga ctctatcatg tcctgcatgg
    20641 cctcatttct cctatgaatg gtatcagtcc ggacgatatc aatctcaaga gccttatgac
    20701 tcgtcttatg gatagtgagg tttcagaagt gattgtggcg actaatgcta cagcggatgg
    20761 tgaagcgact tccatgtatc tttcacgttt gctcaagcca gctggtatca aggttacgcg
    20821 tctagcacga ggtctcgctg tgggagcgga cattgagtat gcggacgaag tgacactctt
    20881 acgagcgatt gaaaatcgga cagagttgta agtgtaggca aatttacgaa ctcccttcat
    20941 ttataaaaaa tcaaagaggc tgaaaatcgt tcctatcggc ctctttttgt atagtgtgat
    21001 gagtaggatc gggttcaagt tttaaaaaac caagcaaata tgatatacta aagagcgagt
    21061 attctagtag aattaggaca aataatatga aacaaacgat tattctttta tatggtggac
    21121 ggagtgcgga acgcgaagtc tctgtccttt cagctgagag tgtcatgcgt gcggtcaatt
    21181 acgaccgttt cacagtcaag actttcttta tcagtcagtc aggtgacttt atcaaaacac
    21241 aggaatttag tcatgctccg gggcaagaag accgtctcat gaccaatgaa accattgatt
    21301 gggataagaa agttgcacca agtgctatct acgaagaagg tgcagtggtc tttccagtcc
    21361 ttcacgggcc aatgggagaa gatggctctg ttcaaggatt cttggaagtt ttgaaaatgc
    21421 cttacgttgg ttgcaacatt ttgtcatcaa gtcttgccat ggataaaatc acgactaagc
    21481 gtgttctgga atctgctggt attgcccaag ttccttatgt ggctatcgtt gaaggcgatg
    21541 atgtgactgc taaaatcgct gaagtggaag aaaaattggc ttatccagtc ttcactaagc
    21601 cgtcaaacat ggggtctagt gtcggtattt ctaagtctga aaaccaagaa gaactccgtc
    21661 aagccttaaa acttgccttc cgatatgaca gccgtgtctt ggttgagcaa ggagtgaatg
    21721 cccgtgaaat tgaggttggc ctcttgggta actacgatgt caagagcacg ctacctggag
    21781 aagttgtcaa ggacgttgcc ttttatgact acgatgccaa gtatattgat aacaagatta
    21841 ctatggatat tcctgccaaa atcagtgatg atgtggtagc tgtcatgcgt caaaatgcag
    21901 aaacagcctt ccgtgccatt ggtggccttg gtctatctcg ttgcgatttc ttctatacag
    21961 ataagggaga gatttttctc aacgagctca ataccatgcc aggtttcacc cagtggtcta
    22021 tgtacccact actttgggac aatatgggga tcagctaccc agaactaatc gagcgtttgg
    22081 ttgaccttgc caaggaaagt tttgacaagc gcgaagcgca tttgatataa aatgaaagag
    22141 agggtagaag ccagaaccat cactgcaagg tgactagagt tctcggactt cagccctttt
    22201 taaaggagta gaaatgaaat taacaatcca tgaagttgcc caagttgttg gagccaaaaa
    22261 tgatatcagt atctttgagg acacccagtt agaaaaagct gagtttgata gtcgtttgat
    22321 tggaactgga gatttatttg tgcccctaaa aggtgtgcgt gacggtcatg actttatcga
    22381 aagagctttt gaaaatggtg cggcagcaac cctgtctgag aaagaggttg caaatcatcc
    22441 ctacattcta gtagatgatg ttttgactgc ctttcaacaa ctagctgcct actatcttga
    22501 aaaaacagct gttgatgtct ttgcggttac gggttcaaat ggcaagacaa cgactaagga
    22561 tatgttggcg catttactgt caacaagata caagacctac aaaacacaag gcaattacaa
    22621 taatgagatt ggccttcctt acacagttct ccacatgcct gaaggaacag aaaagttggt
    22681 cttggagatg gggcaggacc acttgggaga tattcatctc ttgtctgaat tggctcgtcc
    22741 aaaaacagcc atcgtgacct tggttggaga agctcatttg gcctttttca aagatcgttc
    22801 tgagattgct aaaggaaaaa tgcaaattgc agacggaatg gcttcaggtt ccttgctttt
    22861 agcgccggct gaccctatcg tagaggacta tttgccaact gataaaaagg tggttcgttt
    22921 tggtcaagga gcagagctgg aaattactga cttggttgag cgcaaagata gtctgacctt
    22981 caaggccaat ttcttagagc aagtccttga tttgccagta actggcaagt acaatgcgac
    23041 aaatgctatg attgcatcct atgttgcctt gcaagaagga gtttcagagg agcaaattcg
    23101 tttggccttc caacatcttg aattgacgcg taaccgtacc gagtggaaga aagcagccaa
    23161 tggagcagac atcttgtcag atgtttacaa tgccaatcca actgctatga agctgatttt
    23221 agagaccttc tctgccattc cagccaatga aggtggcaag aaaattgcag tgttggcgga
    23281 tatgaaggaa cttggtgtcc agtctgttca acttcataat cagatgattt tgagcctttc
    23341 gccagatgtg cttgatactg ttattttcta cggagaagat attgctgaat tagcccaact
    23401 tgctagtcaa atgttcccaa tcggacacgt ttattacttc aagaaaacag aagaccaaga
    23461 ccaatttgaa gacctagtta agcaggtcaa agaaagcctt ggggctcatg accaaatcct
    23521 gctcaaaggc tctaactcta tgaatctagc caagttggta gaaagtttag aaaatgaaga
    23581 caagtgattt tgtcaagtat ttgcaaagaa tgattgccat tacagatact ggattaacct
    23641 ttacaaaaga tccgtttgac cgtgagcgct acgaagactt gcgagacctt ctatctgaaa
    23701 tgttgaatca agcatcagac cttgattccg aagaagtggc agaagtcttg aagccaactt
    23761 ctgcttatgc gactccctta atggacgtcc gtgcttggat tgttgaggac gaaaagattt
    23821 gtctggttag gggacaagga gaggatagtt gggctttgcc aggtggcttt ggtgaagtcg
    23881 gctattctcc aactgaaaat attctcaagg aaattgaaga agaaaccggt tttaaagcca
    23941 aagttgaaag actgctagct gtttttgata ccaatcgttt ccaactacag agcaaacaat
    24001 atgcaaagtt tgtttttgaa tgtaagcttc tcgatggaca attccaagaa aatcaagaaa
    24061 tcgctgacct tcaatttttt gcaattgacc cactgccgaa tttatctgaa aaacgcatta
    24121 ccaaggagca aatcgagatt ctttggcagg tttatcaagg tcagagggag caatatcttg
    24181 actaagaaga tgattatcgt atttctaaat tcatttttct taagtagcat ggtataataa
    24241 aatgcaggaa aattttgaat catgaggaag actagatgaa tttatgggat attttcttta
    24301 cgactcaggc aaccgagccg cccaaatttg accttttttg gtatgttagc ctatttacgc
    24361 tcttagcctt aaccttttat acagcccatc gctatcgtga aaagaaggtt taccaacgat
    24421 ttttccaaat cttgcagact gttcagttaa tccttcttta tggttggtac tgggtcaatc
    24481 atatgccact gtcagaaagc ctaccctttt accattgccg tatggctatg tttgtggtac
    24541 tcttgcttcc tggtcagtcc aaatataaac aatactttgc attattggga acatttggga
    24601 cattagcagc ctttgtttat ccagtgccag atgcttaccc ttttccacat atcaccattc
    24661 tatcctttat ctttggtcat ttagcactct tggggaactc tctagtttat ctattgagac
    24721 agtataatgc gcgattgctg gatgtgaagg gaatttttct catgaccttt gccctaaatg
    24781 ccttgatttt tgtggtcaat ttggtgacag gtggcgatta cggatttttg acaaaaccgc
    24841 cattggttgg ggatcacggt ctagtagcta attatttact tgtttcaatt gtgctggtag
    24901 ctactatcag tttgactaag aaaatcttag aattcttttt agctcaagaa gcagaaaaaa
    24961 tgattgcaaa ggaagcttaa cacagagctt tcttttttgc tcttagagag tttttacaag
    25021 cagcttataa aataagaatt tctgaataga caaactcaaa aaatggctgg gaaatttagg
    25081 aaaaaagcaa gcacgattaa attttttgtg ttataatatt ttgtgaatag ctatgcctat
    25141 gtttagctat ggaataatac gaagtgcgaa acttggaaga tagagaggaa gcgatgtaat
    25201 ggctagagaa ggctttttta caggtctaga tattggaaca agctctgtca aggtgcttgt
    25261 ggccgagcag agaaatggtg aattaaatgt aattggcgtg agtaatgcca aaagtaaagg
    25321 tgtaaaggat ggaattattg ttgatattga tgcagcagca actgctatca agtcagccat
    25381 ttcccaagcg gaagaaaagg caggcatttc gattaaatca gtaaatgtcg gcttgcctgg
    25441 taatcttttg caggtagaac caactcaggg gatgattcca gtaacatctg atactaagga
    25501 aattacggat caagatgttg aaaatgttgt caaatcagct ttgacaaaga gtatgacacc
    25561 tgaccgtgaa gtcattacct ttattcctga agaatttatt gtggatggtt tccaagggat
    25621 tcgtgaccca cgtggcatga tgggggttcg ccttgaaatg cgtggtttgc tttatacagg
    25681 acctcgtact atcttgcaca atttgcgtaa gacggttgag cgtgcaggtg ttcaggttga
    25741 aaatattatc atttcaccac tagcaatggt tcagtctgtt ttgaatgaag gggaacgtga
    25801 atttggtgct acagtgattg atatgggggc aggtcaaacg actgtcgcta caatccgtaa
    25861 tcaagaactc cagttcacac atattctcca agaaggtgga gattatgtaa ctaaagatat
    25921 ctccaaggtt ttgaaaacct ctcgcaaatt agcggaaggc ttgaaactga attacgggga
    25981 agcctatccg cctcttgcaa gcaaagaaac cttccaagta gaggttattg gagaagtgga
    26041 agcagtcgaa gtgacggaag cctacttgtc agaaattatt tctgcacgaa tcaagcacat
    26101 ccttgaacaa atcaagcaag aattagatag aaggcgtcta ttggacctcc ctggtggtat
    26161 tgtcttaatc ggtgggaatg ccattttacc aggtatggtt gagcttgctc aggaagtctt
    26221 tggcgtccgt gtcaagcttt atgttccaaa tcaagttggt atccgtaatc cagcctttgc
    26281 gcatgtgatt agtttatcag aatttgcggg tcaattaaca gaagttaatc ttttggctca
    26341 gggagcgata aaaggtgaga atgacttaag tcatcagcca attagttttg gtgggatgct
    26401 gcaaaaaaca gctcagtttg tacaatcaac gcctgttcaa ccagctcctg ctccagaagt
    26461 agagtcggtg gcgcctacag aaccaatggc ggatttccaa caagcttcac aaaataaacc
    26521 gaaattagca gatcgtttcc gtggcttgat cggaagcatg tttgacgaat aaagaggaaa
    26581 aataaattat gacattttca tttgatacag ctgctgctca aggggcagtg attaaagtaa
    26641 ttggtgtcgg tggaggtggt ggcaatgcca tcaaccgtat ggtcgacgaa ggtgttacag
    26701 gcgtagaatt tatcgcagca aacacagatg tacaagcatt gagtagtaca aaagctgaga
    26761 ctgttattca gttgggacct aaattgactc gtggtttggg tgcaggaggt caacctgagg
    26821 ttggtcgtaa agccgctgaa gaaagcgaag aaacactgac ggaagctatt agtggtgccg
    26881 atatggtctt catcactgct ggtatgggag gaggctctgg aactggagct gctcctgtta
    26941 ttgctcgtat cgccaaagat ttaggtgcgc ttacagttgg tgttgtaaca cgtccctttg
    27001 gttttgaagg aagtaagcgt ggacaatttg ctgtagaagg aatcaatcaa cttcgtgagc
    27061 atgtagacac tctattgatt atctcaaaca acaatttgct tgaaattgtt gataagaaaa
    27121 caccgctttt ggaggctctt agcgaagcgg ataacgttct tcgtcaaggt gttcaaggga
    27181 ttaccgattt gattaccaat ccaggattga ttaaccttga ctttgccgat gtgaaaacgg
    27241 taatggcaaa caaagggaat gctcttatgg gtattggtat cggtagtgga gaagaacgtg
    27301 tggtagaagc ggcacgtaag gcaatctatt caccacttct tgaaacaact attgacggtg
    27361 ctgaggatgt tatcgtcaac gttactggtg gtcttgactt aaccttgatt gaggcagaag
    27421 aggcttcaca aattgtgaac caggcagcag gtcaaggagt gaacatctgg ctcggtactt
    27481 caattgatga aagtatgcgt gatgaaattc gtgtaacagt tgttgcaacg ggtgttcgtc
    27541 aagaccgcgt agaaaaggtt gtggctccac aagctagatc tgctactaac taccgtgaga
    27601 cagtgaaacc agctcattca catggctttg atcgtcattt tgatatggca gaaacagttg
    27661 aattgccaaa acaaaatcca cgtcgtttgg aaccaactca ggcatctgct tttggtgatt
    27721 gggatcttcg ccgtgaatcg attgttcgta caacagattc agtcgtttct ccagtcgagc
    27781 gctttgaagc cccaatttca caagatgaag atgaattgga tacacctcca tttttcaaaa
    27841 atcgttaagt aaatgaatgt aaaagaaaat acagaacttg tttttcgaga agttgcagag
    27901 gctagtctga gtgctcatcg agagagtggt tcggtctctg tcattgcagt taccaagtat
    27961 gtagatgtac cgacagcgga agccttgctt ccgctaggtg ttcatcatat cggtgaaaat
    28021 cgtgtagata agtttctgga aaaatatgaa gctttaaaag atcgagatgt gacttggcat
    28081 ttgattggta ccttgcaaag acgtaaggtg aaagatgtca ttcaatacgt tgattatttc
    28141 catgcattgg actcagtaaa gctagcaggg gaaattcaaa aaagaagtga ccgagtcatc
    28201 aagtgtttcc ttcaagtaaa tatttctaaa gaagaaagca aacacggttt ttcgagagag
    28261 gaactgctgg aaatcttgcc agagttagcc agactagata agattgaata tgttggttta
    28321 atgacgatgg caccttttga ggctagcagt gagcagttga aagagatttt caaggcgacc
    28381 caagatttac aaagagaaat tcaagagaaa caaattccaa atatgcctat gaccgagtta
    28441 agtatgggaa tgagtcgtga ttataaagaa gcgattcaat tcggttccac ttttgttcgt
    28501 ataggtacat cattttttaa gtaggagaga accatgtctt taaaagatag attcgataga
    28561 tttatagatt attttacgga ggatgaggat tcaagtctcc cttatgaaaa aagagatgag
    28621 cctgtgttta ctccagtaaa ttcttcacag gaaccggctc tcccaatgaa tcaaccttca
    28681 cagtcggctg gtacaaaaga gaacaatatc accagacttc atgcaagaca acaggaattg
    28741 gcaaatcaga gtcagcgtgc aacggataag gtcattatag atgttcgtta tcctagaaaa
    28801 tatgaggatg caacagaaat tgttgattta ttggcaggaa acgaaagtat cttgattgat
    28861 tttcagtata tgacagaggt gcaggctcgt cgttgtttgg actatttgga tggagcttgt
    28921 catgttttag ctggaaattt gaaaaaggta gcttctacca tgtatttgtt gacaccagtg
    28981 aatgttattg taaatgttga agatatccgt ttaccagatg aagatcaaca gggtgagttc
    29041 ggttttgata tgaagcgaaa tagagtacga taatgatttt tttaattcgt atgatttata
    29101 atgcagtgga tatttactcc ctgattttgg tagccttcgc tgtcatgtct tggtttccag
    29161 gtgcctacga atccagttta ggtcgttgga ttgtagcgtt ggtgaaacca gtgcttgctc
    29221 ccttgcaacg cctgccttta cagatagcgg gtcttgattt atctgtttgg gtcgcgattg
    29281 ttttggttcg atttttagga gaaaacctag tgcgttttct ggcgatgata ggatgaataa
    29341 agggatttat cagcatttct ccatagaaga tcgtccattt cttgacaagg gaatggaatg
    29401 gataaagaag gtagaagata gctatgctcc ttttttaact ccttttatca atcctcatca
    29461 ggagaagcta ttaaagattt tggccaaaac ctatggtctt gcttgtagca gtagtgggga
    29521 attcgtctcg agtgagtatg ttcgagtttt attataccca gattatttcc aaccagagtt
    29581 ttcagatttt gaaatatctc tccaggaaat tgtgtattcc aataaatttg aacatttaac
    29641 gcatgctaag attttaggga cagtcatcaa tcaattaggg attgaacgga aactttttgg
    29701 agatatccta gtagatgaag aacgggcgca gattatgatt aatcagcagt ttcttcttct
    29761 ctttcaagat ggactaaaga aaattggtcg tatacctgtt tcgctggagg aacgtccttt
    29821 caccgagaaa atagataagc tagaacagta tcgagaactg gatttatctg tgtctagttt
    29881 tcgattagat gttctttgtt caagttggag acttgattag tgtgagaaaa tttggtcgct
    29941 tgagattact tcaagataag ggacaaacga aaaaagagaa gaaaaaaata accgtccagt
    30001 tattattaag taagtgagga atagaatgcc aattacatca ttagaaataa aggacaagac
    30061 ttttggaact cgattcagag gttttgatcc agaagaagtc gatgaatttt tagatattgt
    30121 ggttcgtgat tacgaagatc ttgtgcgtgc gaatcatgat aaaaatttgc gtattaagag
    30181 tttagaagag cgtttgtctt actttgatga aataaaagat tcattgagcc agtctgtatt
    30241 gattgctcag gatacagctg agagagtgaa acaggcggcg catgaacgtt caaacaatat
    30301 cattcatcaa gcagagcaag atgcgcaacg cttgttggaa gaagctaaat ataaggcaaa
    30361 cgagattctt cgtcaagcaa ctgataatgc taagaaagtc gctgttgaaa cagaagaatt
    30421 gaagaacaag agccgtgtct tccaccaacg tctcaaatct acaattgaga gtcagttggc
    30481 tattgttgaa tcttcagatt gggaagatat tctccgtcca acagctactt atcttcaaac
    30541 cagtgatgaa gcctttaaag aagtggttag cgaagtactt ggagaaccga ttccagctcc
    30601 aattgaagaa gaaccaattg atatgacacg tcagttctct caagcagaaa tggaagaatt
    30661 acaagctcgt attgaggtag ccgataaaga attgtctgaa tttgaagctc agattaaaca
    30721 ggaagtggaa actccaactc ctgtagtgag tcctcaagtt gaagaagagc ctctgctcat
    30781 ccagttggcc caatgtatga agaaccagaa gtagctccag tgcatccgac aggtccaaca
    30841 ccagctacag aaactgttga ttcagcaccg ggatttgaag caccgcaaga atctgttaca
    30901 attttataag aaatattctg agaacaatat cttatcctta tattcccagc gagcaggaga
    30961 tggtgtgagt cctgcattcc ctatcgataa gattatcctc tcaaactatc aagtctgaat
    31021 ctagtaagat ttgacgttcc ccacgttacg ggataagagg gagaaagact aaaacttttt
    31081 ccgaataaag gtggtaccac gattttcgtc ctttttggaa gtcgtggttt ttaatttgtt
    31141 attatttata aaggagatac catgaaactc aaagacaccc ttaatcttgg gaaaactgaa
    31201 ttcccaatgc gtgcaggcct tcctaccaaa gagccagttt ggcaaaagga atgggaagat
    31261 gcaaaacttt atcaacgtcg tcaagaattg aaccaaggaa aacctcattt caccttgcat
    31321 gatggccctc catacgctaa cggaaatatc cacgttggac atgctatgaa caagatttca
    31381 aaagatatca ttgttcgttc taagtctatg tcaggatttt acgcaccatt tattcctggt
    31441 tgggatactc atggtctgcc aatcgagcaa gtcttgtcaa aacaaggtgt caaacgtaaa
    31501 gaaatggact tggttgagta cttgaaactt tgccgtgagt acgctctttc tcaagtagat
    31561 aaacaacgtg aagattttaa acgtttgggt gtttctggtg actgggaaaa tccatatgtg
    31621 accttgactc ctgactatga agcagctcaa attcgtgtat ttggtgagat ggctaataag
    31681 ggttatatct accgtggtgc taagccagtt tactggtcat ggtcatctga gtcagccctt
    31741 gctgaagcag agattgaata ccatgacttg gtttcaactt ccctttacta tgccaacaag
    31801 gtaaaagatg gcaaaggagt tctagataca gatacttata tcgttgtctg gacaacgact
    31861 ccatttacta tcacagcctc tcgtggtttg acggttggtg cagatattga ttacgttttg
    31921 gttcaacctg ctggtgaagc tcgtaagttt gtcgttgctg ctgaattatt gactagcttg
    31981 tctgagaaat ttggctgggc tgatgttcaa gttttggaaa cttaccgtgg ccaagaactc
    32041 aaccacatcg taacagaaca cccatgggat acagctgtag aagagttggt aattcttggt
    32101 gaccacgtta cgactgactc tggtacaggt attgtccata cagcccctgg ttttggtgag
    32161 gacgattaca atgttggtat tgctaataat cttgaagtcg cagtgactgt tgatgaacgt
    32221 ggtatcatga tgaagaatgc tggtcctgaa tttgaaggtc aattctatga aaaggtagtt
    32281 ccaactgtta ttgaaaaact tggtaacctc cttcttgccc aagaagaaat ctctcactca
    32341 tatccatttg actggcgtac taagaaacca atcatctggc gtgcagttcc acaatggttt
    32401 gcctcagttt ctaaattccg tcaagaaatc ttggacgaaa ttgaaaaagt gaaattccac
    32461 tcagaatggg gtaaagtccg tctttacaat atgatccgtg accgtggtga ctgggttatc
    32521 tctcgtcaac gtgcttgggg tgttccactt cctatcttct acgctgaaga tggtacagct
    32581 atcatggtag ctgaaactat tgaacacgta gctcaacttt ttgaagaaca tggttcaagc
    32641 atttggtggg aacgtgatgc caaagacctc ttgccagaag gatttactca tccaggttca
    32701 ccaaacggcg agttcaaaaa agaaactgat atcatggacg tttggtttga ctcaggttca
    32761 tcatggaatg gagtggtggt aaaccgtcct gaattgactt acccagccga cctttaccta
    32821 gaaggttctg accaataccg tggttggttt aactcatcac ttatcacatc tgttgccaac
    32881 catggcgtag caccttacaa acaaatcttg tcacaaggtt ttgcccttga tggtaaaggt
    32941 gagaagatgt ctaaatctct tggaaatacc attgctccaa gcgatgttga aaaacaattc
    33001 ggtgctgaaa tcttgcgtct ctgggtaaca agtgttgact caagcaatga cgtgcgtatc
    33061 tctatggata tcttgagcca agtttctgaa acttaccgta agattcgtaa cactcttcgt
    33121 ttcttgattg ccaatacatc tgactttaac ccagctcaag atacagtcgc ttacgatgag
    33181 cttcgttcag ttgataagta catgacgatt cgctttaacc agcttgtcaa gaccattcgt
    33241 gatgcttacg caaactttga attcttgaca atctacaagg ccttggtgaa ctttatcaac
    33301 gttgacttgt cagccttcta ccttgatttt gccaaagatg ttgtttacat tgaaggtgcc
    33361 aaatcactgg aacgccgtca aatgcagact gtcttctatg acattcttgt caaaatcacc
    33421 aaactcttga ctccaatcct tcctcacact gcggaagaaa tctggtcata tcttgagttt
    33481 gaagccgaag acttcgtcca attgtcagaa ttaccagaag ttcaaacttt tgctaaccaa
    33541 gaagaaatct tggatacatg ggcagccttc atggacttcc gtggacaagc acaaaaagcc
    33601 ttggaagaag ctcgtaatgc aaaagttatc ggtaaatcac ttgaagcaca cttgacagtt
    33661 tatccaaatg aagttgtgaa aactctactc gaagcagtaa acagcaatgt agctcaactt
    33721 ttgatcgtgt ctgagttgac catcgcagaa gaaccagctc cggaagctgc ccttagcttc
    33781 gaagatgtag tcttcacagt tgaacgcgct gcaggtgaag tatgtgaccg ttgccgtcgt
    33841 attgacccaa caacagcaga acgtagctac cacgcagtca tctgtgacca ctgtgcaagt
    33901 atcgtagaag aaaactttgc agatgcagtc gcagaaggat ttgaagagaa ataagattga
    33961 aaagtctagg taaaactcaa tttgagaaga aaagacaact aattttatag tctattaaac
    34021 gcattgtatc acgtttttga acacctgata tgatgcgttt tttatttatt ttaaaaattt
    34081 gcgaggtatg actttttata ctcaacaaga atcaaagaga aacttagcaa gctaacagta
    34141 gtaatataaa ataggaattt gatattaggg ataagattgg taaatagtgt aatattttta
    34201 caacaataaa tttatatagt tatttctggt ttctgaaaag tattatatta tattttattt
    34261 catattatac aaatttttat tttataatat cagaaaatac tttttttaaa aagcaaatat
    34321 gatacaattt tatttgaaaa aaaataaaaa aggagatttt attatgaaat caagtggtat
    34381 cgtcggtctt gtgggaggaa ttttacttct tatttgtcct tttgtcttgt tgggaacagc
    34441 ggtaaataca gctgctacaa ctcttaatgg aggagctact gcaggggctt tttcaggtgt
    34501 tgccttactc ttgaatgcct tgaagattgc aaatcttgtt cttggtatca ttgctattgt
    34561 ttactataaa ggagataagc atgtaggtgc agctccgtct gtactaatga ttgtttctgg
    34621 tgaagttagt ctcattccat tcttaggatg ggttgggggg attcttgcta ttatcggagg
    34681 atctctattc cttgcaacat tgaagaaatt caaatcagaa gaataaaagg tattttagca
    34741 tgaaaagaac aaaaaagttt atcggtatag gagtagctct attatctctt tctcttctag
    34801 ttgcatgtgg aacataaagt tcaaagaata cttcaacaag taatgatgag aagacagtag
    34861 caacatccaa tagttcaaaa gaaacaatca ctttcgatac accggttgta acagacgatg
    34921 cgattgaatc aatacgcact tatgcagatt atatagatct ttataaaaat atttttgatg
    34981 attattttac taaagctgag gaaggtttca aaggcacaac tatggaaaat aatgactcgt
    35041 ttactaaact aaaagagtca actcaaaaat tattcgatgc gcagaaaaaa aggttaaata
    35101 atgaagatag aatagaaaca accaaaaaca atgtgattgc caaacattgt caaacagtcc
    35161 tttccttttt ggttttgact agcttttttg tgaaaaattg tgtaaaatag aatagataaa
    35221 cgaggggaaa cctcggaaaa tttaaaggag aatccatcta atggtaaaat tggtttttgc
    35281 tcgccacggt gagtctgaat ggaacaaagc taaccttttc actggttggg ctgatgttga
    35341 tttgtctgaa aaaggtacac aacaagcgat tgacgctggt aaattgatca aagaagctgg
    35401 tatcgaattt gaccaagctt acacttcagt attgaaacgt gctatcaaaa caactaactt
    35461 ggctcttgaa gcttctgacc aattgtgggt tccagttgaa aaatcatggc gcttgaacga
    35521 acgtcactac ggtggtttga ctggtaaaaa caaagctgaa gctgctgaac aatttggtga
    35581 tgagcaagtt cacatctggc gtcgttcata cgatgtattg cctccaaata tggaccgtga
    35641 tgatgagcac tcagctcaca cagaccgtcg ttatgcttca cttgacgact cagtcattcc
    35701 agatgctgaa aacttgaaag tgactttgga acgtgctctt ccattctggg aagataaaat
    35761 cgctccagct cttaaagatg gtaaaaacgt attcgtagga gctcacggta actcaatccg
    35821 tgcccttgta aaacacatca aaggtttgtc agatgatgag atcatggacg tggaaatccc
    35881 taacttccca ccattggtat tcgaattcga cgaaaaattg aacgttgttt ctgaatacta
    35941 ccttggaaaa taaaaaattg taagcctagg attgatttct aggcttttta tgttagtatg
    36001 gaagtatgat aaggaataaa aaacaagatt atgtactggc ctacaagcaa ccagcttcaa
    36061 ccacttacat gggttgggaa gaagaagctt taccgatagg caatggttct ttaggagcaa
    36121 aagtatttgg ccttataggg gctgaacgga ttcaatttaa tgaaaaaagt ctctggtctg
    36181 gaggtccact tcctgatagt tcagattatc agggtggaaa tcttcaggat cagtatgttt
    36241 ttttagctga gattcggcag gctttggaga agagagatta caatctggct aaggaactgg
    36301 ctgagcagca cctaattggg ccaaaaacga gtcaatatgg gacctatctg tcttttgggg
    36361 atattcacat tgagttcagc cagcaaggta cgactttgtc tcaggtgacg gactatcaga
    36421 gacagctgaa tattagtaag gcacttgcga cgacttctta tgtctataag ggaacgcgat
    36481 ttgaacgtaa agcttttgcg agttttccag atgatctctt gattcaatgt tttactaagg
    36541 aagggttgga aactctagat tttactatag aactatcctt gacctgtgat ttggcttctg
    36601 atggaaagta tgagcaggaa aaatctgatt acaaggagtg taagttggat attactgatt
    36661 ctcatatctt gatgaaggga agagttaagg ataatgatct gcggtttgct agttatctag
    36721 cttgggaaac ggatggagat attagagttt ggtcagatag ggttcagata tcaggagcca
    36781 gttatgccaa tctcttcttg gccgctaaga cggattttgc ccaaaatcct gctagcaatt
    36841 atcgcaagaa actagattta gagcaacagg tgatagactt ggtggacaca gctaaagaaa
    36901 agggctatac ccaattgaaa tcaaggcata tcgaggacta ccaagcctta ttccagcgtg
    36961 ttcaattgga tttggaagct gatgttgacg catccactac agatgatttg ttaaaaaatt
    37021 ataagccaca agaagggcag gctttggagg agctgttctt ccagtatgga cggtatttat
    37081 tgattagttc gtccagagac tgcccagatg ctctaccagc taacctacag ggagtctgga
    37141 atgcggtcga caatcctcct tggaattcgg actatcactt aaatgtcaat ctgcagatga
    37201 attattggcc agcctatgtt accaatctcc tagagacggt ctttccagtc atcaactatg
    37261 tagatgattt gcgtgtctat ggtcgtctag cggctgtaaa gtatgcagga atcgtctctc
    37321 aaaaaggtga ggaaaatggt tggttggttc atactcaagc gactcccttt ggttggacgg
    37381 cacctggttg ggattactat tggggttggt caccagctgc caatgcgtgg atgatgcaaa
    37441 ccgtttatga agcctattca ttttataggg accaagacta tctcagggag aaaatttatc
    37501 ccatgttgag ggaaacggtt cgtttttgga atgccttttt acataaggat cagcaggcgc
    37561 agcgttgggt ctcttctccg tcttattccc cagaacatgg gccgatttcg attggcaata
    37621 cctatgacca atctctgatt tggcagttat ttcatgattt tattcaggct gctcaggaat
    37681 tgggactgga tgaggacttg ttgactgagg ttaaggagaa gtctgattta ctaaatcctt
    37741 tgcaaatcac tcaatctggt cgaatcaggg agtggtatga ggaggaagag cagtattttc
    37801 aaaatgagaa agtggaggcc cagcatcggc acgcttccca tctagtggga ctctattctg
    37861 gcaatctctt tagctacaag ggacaagagt atattgaagc ggcgcgtgct atcctcaatg
    37921 atcgtggaga tggcggcaca ggctggtcca aggctaataa gatcaatctc tgggcgcgtt
    37981 tgggagatgg caatcgagcc cataaattat tggcagagca gttaaagaca tccaccttgc
    38041 caaatctttg gtgtagccat cctccttttc agatagatgg taattttggt gctactagtg
    38101 gcatggcaga aatgttactc cagtctcatg cagcttatct ggtacctcta gctgccctac
    38161 ctgatgcttg gtcaacaggt tctgtttcag gcttaatggc acgtggacat tttgaagtga
    38221 gcatgagctg ggaagataaa aaactcttac agttgaccat tttatcaagg agtggaggag
    38281 atttgcgagt ttcttatcca gatattgaga agagtgtgat taaaatgaat caagaaaaaa
    38341 taaaagcgaa atgcatgggg aaagattgta tttcggtggc aacagcagaa ggtgatcttg
    38401 ttcaatttta tttttaagaa gatgttataa ggcagtaatt tgaaacttcc ttttaataag
    38461 gatttgagaa tataggcagt ttccaactag ttgaaaaagc gttataatga taataggaag
    38521 taatactcaa tgaaaatcaa agagc
//
LOCUS       DAVBHS010000004        34001 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVBHS010000004 DAVBHS010000000
VERSION     DAVBHS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34001
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.004"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(132..1118)
                     /locus_tag="VQ651_000119"
     CDS             complement(132..1118)
                     /locus_tag="VQ651_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2753175.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(1128..1928)
                     /locus_tag="VQ651_000120"
     CDS             complement(1128..1928)
                     /locus_tag="VQ651_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HET2753176.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRIYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(2189..3031)
                     /locus_tag="VQ651_000121"
     CDS             complement(2189..3031)
                     /locus_tag="VQ651_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2753177.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(3033..4325)
                     /locus_tag="VQ651_000122"
     CDS             complement(3033..4325)
                     /locus_tag="VQ651_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2753178.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGNKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(4436..5707)
                     /locus_tag="VQ651_000123"
     CDS             complement(4436..5707)
                     /locus_tag="VQ651_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2753179.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            6257..7774
                     /gene="malQ"
                     /locus_tag="VQ651_000124"
     CDS             6257..7774
                     /gene="malQ"
                     /locus_tag="VQ651_000124"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET2753180.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            7800..10058
                     /locus_tag="VQ651_000125"
     CDS             7800..10058
                     /locus_tag="VQ651_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HET2753181.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(11102..11674)
                     /locus_tag="VQ651_000126"
     CDS             complement(11102..11674)
                     /locus_tag="VQ651_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753182.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAINLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(12296..13144)
                     /locus_tag="VQ651_000127"
     CDS             complement(12296..13144)
                     /locus_tag="VQ651_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET2753183.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            13289..13567
                     /locus_tag="VQ651_000128"
     CDS             13289..13567
                     /locus_tag="VQ651_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753184.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            13569..15632
                     /locus_tag="VQ651_000129"
     CDS             13569..15632
                     /locus_tag="VQ651_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET2753185.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(15710..16966)
                     /locus_tag="VQ651_000130"
     CDS             complement(15710..16966)
                     /locus_tag="VQ651_000130"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET2753186.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            17108..19573
                     /locus_tag="VQ651_000131"
     CDS             17108..19573
                     /locus_tag="VQ651_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET2753187.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            19638..20549
                     /locus_tag="VQ651_000132"
     CDS             19638..20549
                     /locus_tag="VQ651_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET2753188.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCLEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVSVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            20779..21477
                     /gene="dapD"
                     /locus_tag="VQ651_000133"
     CDS             20779..21477
                     /gene="dapD"
                     /locus_tag="VQ651_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HET2753189.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVCVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            21545..22675
                     /locus_tag="VQ651_000134"
     CDS             21545..22675
                     /locus_tag="VQ651_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HET2753190.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFTSQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            22687..23226
                     /locus_tag="VQ651_000135"
     CDS             22687..23226
                     /locus_tag="VQ651_000135"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET2753191.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLTPKTYPKGRMDFVVYDPQQLVKTSFALLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGRALSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            23210..23887
                     /locus_tag="VQ651_000136"
     CDS             23210..23887
                     /locus_tag="VQ651_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET2753192.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(23959..24858)
                     /gene="galU"
                     /locus_tag="VQ651_000137"
     CDS             complement(23959..24858)
                     /gene="galU"
                     /locus_tag="VQ651_000137"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET2753193.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(24880..25896)
                     /locus_tag="VQ651_000138"
     CDS             complement(24880..25896)
                     /locus_tag="VQ651_000138"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET2753194.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     YHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKAI
                     IMHASKGLEPDSHKRLSTILEEEIPEQLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            26108..26485
                     /locus_tag="VQ651_000139"
                     /pseudo
     CDS             26108..26485
                     /locus_tag="VQ651_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(26665..27090)
                     /locus_tag="VQ651_000140"
     CDS             complement(26665..27090)
                     /locus_tag="VQ651_000140"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2753195.1"
                     /translation="MNNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKSLTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(27158..27808)
                     /gene="phoU"
                     /locus_tag="VQ651_000141"
     CDS             complement(27158..27808)
                     /gene="phoU"
                     /locus_tag="VQ651_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET2753196.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(27823..28575)
                     /gene="pstB"
                     /locus_tag="VQ651_000142"
     CDS             complement(27823..28575)
                     /gene="pstB"
                     /locus_tag="VQ651_000142"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2753197.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDVLLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(28577..29392)
                     /gene="pstA"
                     /locus_tag="VQ651_000143"
     CDS             complement(28577..29392)
                     /gene="pstA"
                     /locus_tag="VQ651_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET2753198.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(29385..30248)
                     /gene="pstC"
                     /locus_tag="VQ651_000144"
     CDS             complement(29385..30248)
                     /gene="pstC"
                     /locus_tag="VQ651_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET2753199.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(30366..31241)
                     /locus_tag="VQ651_000145"
     CDS             complement(30366..31241)
                     /locus_tag="VQ651_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HET2753200.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(31408..32739)
                     /locus_tag="VQ651_000146"
     CDS             complement(31408..32739)
                     /locus_tag="VQ651_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HET2753201.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSALKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(32740..33447)
                     /locus_tag="VQ651_000147"
     CDS             complement(32740..33447)
                     /locus_tag="VQ651_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2753202.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(33531..33992)
                     /locus_tag="VQ651_000148"
     CDS             complement(33531..33992)
                     /locus_tag="VQ651_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET2753203.1"
                     /translation="MIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALYTSIYFLLE
                     ETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQLHYCVPKGT
                     IWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
BASE COUNT        10022 a         6414 c         7280 g        10285 t
ORIGIN      
        1 aagatcttgg gaaaagactc aatttcagta gaaaaggaag taaatcttcc cacattaaaa
       61 cgcatagtat caagtttttt caatacctga tagtatgcgt ttttgctttt aacgactttt
      121 tgcccagtct cttatttttc gataattttg tgggcgatca attgacggta acaaatttgt
      181 ttattgtttt tattatcatt aataatctgg agaatagttt caagggaaac acgaccaagc
      241 tctaaactat tgatatcgac ataggctgcc aagttgagct tgggattaac cgagtcaaag
      301 ctgagaacag ggacatccag ctggtgtttg gcaatatagt tacaaacgcc ttcagctagg
      361 aggctatcgg ttgtgatgat agcatcaatt tgtggatcgt gcttgaataa tcgcttgcta
      421 aatttatagc ccttttcttc cagaaactcg tcggcaaagt agatgcgatt gttgtcagtg
      481 gtaagtttgt aatgtttaag cgcctgttca tagcctgtta aacggtcttt ggtcacgaag
      541 agctttttac ttcctccgat aaaggcaatg cgtttgcagc cttttttgat gaaatattca
      601 gtcgcatcaa aaccagcttg aacattgtcg ttgtcgacaa gtgggatgaa aggagataga
      661 gatttaccta agataaggaa ggggaactgt tcttctgcga cgagttttac gagagggtct
      721 tcttcttggg catagagaaa gattagccca tctacacgct tgccgtagac catttgtgaa
      781 atagcgttga gacgctcctt ctcatctttc cctgttgcta tctgaatggc atagtggttt
      841 tcagatgcga cttgagagat gccacgtaga accgatggaa agaaaggatt ctggtagaag
      901 gcgtctgagt catcaggaag aactaatccg ataacctgag tatagctgct taccaagcta
      961 cgagcgttga ggtttgggtg gtagttgagt tccttcatag ctttgcgaac gcgttttttt
     1021 gtttcgtcgc taatggttga tttattttga ataacacggg ttacggttga aggcgaaaca
     1081 ccagcagcct tggccacgtc tttaatcgta acgggcataa aaatctccta tttatggtaa
     1141 tttggatcac ggaaatgtgt tttataaaag atagtgacca gatacagaac aaaaagaata
     1201 ttttgtacag taatcagggt tgtcatattt tggccaaata atcccaaaat aagtgtaatc
     1261 agagtcggca atcctaaaca gttcaagata aagtgatagc actctttaaa ggttctaaat
     1321 gaaaagaggc gtgatttctt agtgatataa aggagaagac tagctcctag agagacgata
     1381 aagaaattca aaccaaagag gaagctcgca ccgagaacta ggaagaggct gatatagata
     1441 cgattttgtt ggtaccagtc tttagaaatt gcttgggtca agctgtcttt gcttttgaaa
     1501 ctctcagtct gaatggctcg gtaagagatg cgggtcagtt ccttgctttc cttgctgatg
     1561 actagttcat ttgtatcgaa atgcagttgc aagtccttag gtaattcctt gatttgactt
     1621 ggaccaatca caacagaagg ggcttgacta gctgttccag tataagttaa tgtgccatcg
     1681 acaattgtag catgttcaga gagatcctgg acaacctcat ttgtcagagg ttcatagaca
     1741 ttatcgataa aagtttctag cggataggtc tcctgggagc tgttttggat agcaatgggt
     1801 accatagaca agctgataag gaagatactg gtaaagagaa gttgaaacca gttgagctcg
     1861 aaacgtttgg acaggggctt acgaaacccc caaatacttg aaaaatagga aaatggatat
     1921 ggaagcatag gtatctttct aaaaggtgtt ttctatatga gtattattat atagagaaat
     1981 gcgctttatt tcaagtctag gagaaaaatt gcttgctaca aggatattat ttacagtgac
     2041 aattgctaat aatcaatgaa aatcaaagtg caaaccagga agctagctgc aggttgctca
     2101 aagcacaact ttgaggttgg agataaagct gacgtggttt gaagagattt tcgaagagta
     2161 taaaatgaaa aagggtggcg gggataaatt atcccttgtc gccaccactt gtaagtcctg
     2221 aaacaaagtt cttttgtaga aagaagaaga gaatacagat tggaagggcg atgaggatag
     2281 cacctgctga gaagtaggca atcttcaagt ttttcgcatt gttaacgaag gtttggagac
     2341 ctacggcaac agtaaagtat tctttctcac gaagcaagaa actagagagg atgtagtccc
     2401 cgaaaggtcc catgaaggcc cagagagctt gtacggcaac cattgggcga acaagtggta
     2461 gaacaatttg ccagaagcgg cggaagtgtc ctgcaccgtc tagttttgca gattcgtcta
     2521 aagacattgg cactgtatcg aagtagcctt tcatgagcca agcattcatc gggataccac
     2581 caccaacgta gaggaagatg aggaaccagt tgtggttaag ggcgttcaac ataagcgcca
     2641 taacgaagaa ggctgtcaaa gcggccatag ttggcaccat ttggatgatc aagaagaaga
     2701 ccaaactttg tttacgagcc aagaagttgt aacggctgta agcataacca gcaagtacga
     2761 tgatacttgt ttgaacagcc atggtaatta aggcgataat caaagtgttg aggtaccaag
     2821 taccgtacaa ggtttcagtg aagaggcctt taaagttatc aaaattgagg tcgatattag
     2881 tatctagttt aaaggctgag acgttacctg ctttaaaggc tgacataatg gtaatcaaca
     2941 gtggatagat aattacaatt gatagaccaa tcaggtaaag gtaagtaagg ctttgagtca
     3001 gtctacgttt gagtttaatt gagttattca tcttagacgt cctccatatc aaatgcgtgt
     3061 agtttcttga atgcgatcat agagattgag atgacaatga tagagataat caaggtaaca
     3121 gctgccgcca ttgagtattg aggagatgta cctgttgtca aacggtagat ccatgagatc
     3181 aagatatcgg ttgaaccagc tccacctccg acactaccag gtcctccacc attgaagagg
     3241 tacatgatag agaagttgtt aaagttgaag gtgtattggc taatcaaagt aggtgccgca
     3301 acagccaaaa tcattgggaa agtgatgttg cggaattttt gccaagcgtt ggcaccgtca
     3361 atataagctg cttcgtaaag gtcgttagga atagattgca agatacccaa agtcaaaacg
     3421 tagatgtatg ggaatccgag ccaaccttgc atcataatca aggcaatctt agtccaagtt
     3481 gggtctgttt tccaaggaat aagagctcca tcaaggaaag gaaggaattt agccaagatt
     3541 ggcaatactt gagtgttgat agcaccgaca ctatcgttaa acatgtttga gaatgtcaag
     3601 atagtgatga aggctgggac agcccaagga agaaggaaaa taacaccaaa gatacgtttt
     3661 cctttgataa atggttggtt ggcaatgata gctgtgaaga taccaattac gatttgtaaa
     3721 gtagaagctg ccaaagccca aatgatagtc caagaaagaa cagaaccaaa ggcagaacgg
     3781 aaggtactca agctccaaat gtttgtaaag ttggtcaaac caacccagtc caacaacttg
     3841 tttggtggca agtgttggaa gtcgtagttg gtaaaggcga tcatcaaggt tacgataact
     3901 gggaagataa tcgcgaaggt catggcaaca taagatggaa tgatcaagag gtaagggaag
     3961 ccattttcat aaatcccttt gatcatgtct ttgagtgtgc gtggaactgg aattccattg
     4021 ttaatgcgtt ttgcaatcgt atgtgcatct ttgatatttg agaaataaaa gagtacataa
     4081 acgattacaa agattagatg gaaggcacca cgaatcagca taaagaggga attatcacga
     4141 cctggtttgt taccaagagt gatgaggttg ctcaattcag gggttgcaag tgctaggaag
     4201 taaaggacaa atacgatggt tacaccaagg aagataaaac ctttggcttt ttgtttattg
     4261 taaatctgtc ctaacccagg aatgatagac agcagggctg ctttactagg ttgttgcttt
     4321 tccatattaa ctcctttcat aggatactac ttttttataa gaaagctaaa ttatttgtgt
     4381 ctctattgat aaattcaaag ggggatttga tttccacccc ccttgaacaa attttttatt
     4441 caccaaattt ttgtttgatt gtttctttga tcaatgttac agcatcgtta gcagctgttt
     4501 tagcatcttt ttgaccactt acagcatcaa agagcatatt tttcgctgga tcccaaactg
     4561 cagacatttg agagatgttt ggcagtggtt gagtgttctt gaactgtttg ataacagctg
     4621 ttgtcaactc atcgttttta ccttcagcgt atgaacgagc ctcagtatta gctgggattt
     4681 cgttagtctt atcatataat actttttgtt gttcagttgc aacaaggaag tctacaaatt
     4741 tttgagaagc ttcaaggttc ttaacggctt gaggaatgac ccaagcttta ccaccaccga
     4801 atgcagcata ttcttttcca tttggaagag ttgggatagt tgcaactccg tagtttactt
     4861 tagcatcttt aaaggcttga gctttccaag gtccgtcgat gatagcagct gttttacctt
     4921 cttggaattg agtttggatt aagtttccag caccttctgt atcttgcata cctttaggcc
     4981 atttttcgta ccaagattta gcgtaattga tacctacgat agaaccgtcg tttgcaagac
     5041 cgatgtcttt agcgtcttta ccgttttggc caaagacgta agcaccgtta ccggcaagaa
     5101 gtccatatgt atagtagaag tttgtccagt cagctaggaa ggcagtagtt ttaccatctt
     5161 caccagcgaa tgcgtatttg ctatctttag caaggttttc caagtcagca aatgtttttg
     5221 gagcatcttt caccaagtct ttgttgtagt acataacaag tgactcgata acggcaggag
     5281 caccgtaaac tttaccatta gcagctgtta caagagattt agttgtgtcg tctgttttag
     5341 caccatcgct caatttcact tctgaaagtt gtccgtcaga accaaggcta cctacacggt
     5401 cgtatggagc catcataaca tcagggacat taccagattg gttgtcaaga gaaagtttat
     5461 caagacctcc tagagcatca ccagttttaa gagtgacttt tactccagct tctttttcat
     5521 aagctttagc aacctcttca atatagctct tatatccctc gtctacatat acagtgagtt
     5581 ctttgacttc agatgaacca gaatcagcag gcttatcagc agttttgctt ccgcaagcta
     5641 ccaaaagcaa gctagcaagt gtagcagttc caagcaccgc agtgctcttc ataaatttag
     5701 atgacatagt gtattcctcc taaagaatag caagttttat tgataaggaa acgcaaacgt
     5761 tttcctttat gagcttagta tagcacaaat agaaaacggt tgcaagtatt ttttgtaaaa
     5821 attttaaaaa atttttaagc ttgatttgat agcataattt tgcattttaa tagaagaaaa
     5881 gtatgaaata gataagaata cagtattttt aataaaaata tatggaatct gtctaaagag
     5941 ggaaatcagg aggggctacc tccctgtttc taatctatat ctattttcta tagacgtttg
     6001 tgctttgtta catataaatt caagctccct aaggaggttt tatactttgt agactgtctc
     6061 gtgagagtca aaccttagga ttagatagtt ttaagttatc tttgctaaaa tgagttagtt
     6121 tccttaaaaa taatcaaaaa agttttttaa aaacgcttgc aattatgctt gaaaaggagt
     6181 atacttataa gtaacgcaaa cgtttgcgtc tgcaaaatac gcaacgttcc attattttaa
     6241 cacacgaggt gctattatga aaaaacgtca aagtggtgtg ttgatgcaca tctcttctct
     6301 tccaggagct tacggaatcg gatcatttgg tcaaagtgct tacgacttcg ttgatttctt
     6361 ggtccgtaca aaacaacgtt actggcaaat ccttccatta ggagcaacta gttacgggga
     6421 ttctccttac caatctttct cagccttcgc aggaaacact cattttatcg atttagatat
     6481 cttggtggag caaggtttgt tggaagcaag tgaccttgaa ggagttgact ttggtagcga
     6541 tgcgtctgaa gttgactatg ctaaaatcta ctatgcacgt cgtcctcttt tagaaaaagc
     6601 ggtgaaacgt ttctttgaag tcggagatgt taaagatttt gagaaatttg ctcaagacaa
     6661 ccaatcatgg cttgagctct ttgctgagta tatggctatc aaagagtatt ttgacaatct
     6721 tgcttggact gaatggccag atgcagatgc tcgtgctcgt aaagcttcag cacttgaaag
     6781 ctatcgtgag caattggcag acaagttggt ttaccaccgt gtgactcaat acttcttctt
     6841 ccaacaatgg ttgaaattga aagcttacgc taacgacaac cacatcgaaa tcgttgggga
     6901 catgccaatc tacgtagcgg aagattcaag tgatatgtgg gcaaatccac atctcttcaa
     6961 aacagatgtc aatggtaagg ctacttgtat cgcaggatgc ccaccagatg agttttctgt
     7021 aactggtcag ctttggggta acccaatcta tgactgggaa gcaatggaca aagacggcta
     7081 caaatggtgg attgaacgct tgcgtgaaag cttcaaaatc tacgatatcg ttcgtatcga
     7141 ccacttccgt ggcttcgaat cttactggga aatccctgct ggttccgata cagcagcacc
     7201 tggtgagtgg gtgaaaggtc caggctacaa gctttttgca gccgttaagg aagaacttgg
     7261 tgagctaaac atcatcgcag aagaccttgg cttcatgaca gatgaagtga tcgaattgcg
     7321 tgaacgtact ggcttcccag gaatgaagat tcttcaattt gccttcaacc cagaagacga
     7381 aagcattgat agcccacact tggcacctgc taactcagtt atgtacacag gaacacacga
     7441 taacaatacg gttcttggtt ggtaccgtaa tgagattgat gatgcgactc gtgagtacat
     7501 ggctcgttac acgaaccgta aagaatacga aacagtggta cacgctatgc ttcgtacagt
     7561 attttcatca gttagcttta tggcaattgc aactatgcaa gatttactag aattggatga
     7621 ggcagctcgt atgaacttcc catctaccct tggtggaaac tggtcttggc gtatgactga
     7681 agatcaattg acaccagctg tcgaggaagg tttgcttgac ttgacaacaa tttatcgccg
     7741 aattaatgaa aatttggtag atttaaagaa ataagacaat aatcaggaga caactaaaca
     7801 tgttatcact acaagaattt gtacaaaatc gttacaataa aaccattgca gaatgtagca
     7861 atgaagagct ttaccttgct cttcttaact acagcaagct tgcaagcagc caaaaaccag
     7921 tcaacactgg taagaaaaaa gtttactaca tctcagctga gttcttgatt ggtaaactct
     7981 tgtcaaacaa cttgattaac cttggtcttt acgacgatgt taaaaaagaa cttgcagctg
     8041 caggtaaaga cttgatcgaa gttgaagaag ttgaattgga accatctctt ggtaatggtg
     8101 gtttgggacg tttggctgcc tgctttatcg actcaattgc tactcttggt ttgaatggtg
     8161 acggtgttgg tcttaactac cactttggtc ttttccaaca agttcttaaa aacaaccaac
     8221 aagaaacaat tccaaatgca tggttgacag agcaaaactg gttggttcgc tcaagccgta
     8281 gctaccaagt accatttgca gactttactt tgacatcaac tctttacgat attgatgtta
     8341 ctggttatga aacagcgact aaaaaccgct tgcgtttgtt tgacttggat tcagttgatt
     8401 cttctattat taaagatggt atcaactttg acaagacaga tatcgctcgc aacttaactc
     8461 tcttccttta cccagatgat agtgaccgtc aaggtgaatt gctccgtatc ttccaacaat
     8521 acttcatggt ttcaaacggt gcgcaattga tcatcgacga agcaatcgaa aaaggaagca
     8581 acttgcatga ccttgctgac tacgcagttg tccaaatcaa cgatactcac ccatcaatgg
     8641 tgattcctga attgattcgt cttttgactg cacgtggtat cgatcttgac gaagcaatct
     8701 caattgttcg tagcatgact gcctacacta accacacaat ccttgctgaa gcgcttgaaa
     8761 aatggcctct tgaattcttg caagaagtgg ttcctcactt ggtaccaatc atcgaagaat
     8821 tggaccgtcg tgtgaaggca gagtacaaag atccagctgt tcaaatcatc gatgagagcg
     8881 gacgtgttca catggctcac atggatatcc actacggata cagtgttaac ggggttgcag
     8941 cactccatac tgaaatcttg aaaaattctg agttgaaagc cttctacgac ctttacccag
     9001 aaaagttcaa caacaaaaca aacggtatca ctttccgtcg ttggcttatg catgctaacc
     9061 caagattgtc tcactacttg gatgagattc ttggagatgg ttggcaccat gaagcagatg
     9121 agcttgaaaa actgttgtct tatgaagaca aagcagctgt caaagaaaaa ttggaaagca
     9181 tcaaggctca caacaaacgt aaattggctc gtcacttgaa agaacaccaa ggtgtggaaa
     9241 tcaatccaaa ttctatcttt gatatccaaa tcaaacgtct tcacgagtac aaacgccaac
     9301 aaatgaacgc tttgtacgtg atccacaaat accttgacat caaagctggt aacatccctg
     9361 ctcgtccaat cacaatcttc tttggtggta aagcagctcc agcctacaca atcgctcaag
     9421 atatcattca cttgatcctt tgcatgtcag aagttattgc taacgatcca gcagtagctc
     9481 cacacttgca agtagttatg gttgaaaact acaacgttac tgcagcaagc ttccttatcc
     9541 cagcatgtga tatctcagaa caaatctcac ttgcttctaa agaagcttca ggtactggta
     9601 acatgaaatt catgttgaac ggagctttga cacttggtac tatggacggt gctaacgtgg
     9661 aaatcgctga gttggttgga gaagaaaaca tctacatctt cggtgaagat tcagaaactg
     9721 ttatcgacct ttacgcaaaa gcagcttaca aatcaagcga attctacgct cgtgaagcta
     9781 tcaaaccatt ggttgacttc atcgttagtg atgcagttct tgcagctgga aacaaagagc
     9841 gcttggaacg tctttacaat gaattgatca acaaagactg gttcatgact cttcttgact
     9901 tggaagacta catcaaagtc aaagagcaaa tgcttgctga ctacgaagac cgtgacgcat
     9961 ggttggataa agtcatcgtt aacatttcta aagcaggatt cttctcatct gaccgtacaa
    10021 tcgctcagta taacgaagac atctggcact tgaactaata ctcttcgaaa atctcttcaa
    10081 accacgtcag ctttatctgc aacctcaaag cggtgctttg agcaacctgc ggttagcttc
    10141 ctagtttact ctttgatttt cattgagtat aagatacaaa tttatactaa tacatcttgt
    10201 aaaaaagcga gtttcgattg aaattcgctt ttttaatgat gtagatttgg gtcaatcttg
    10261 tctaaaaata gggaaatcct agatacagtg aaggctttaa atgctggttt ttactgtcct
    10321 cagccttata ttttttcgta gttgattacc tcatacctct tgtattcgct tacataaagt
    10381 attataatat aattgtagga aagaaggtgt ttttatgata tacacactta aattggtgtt
    10441 gtttattacc tttcttgtaa taagcttgtt acctgataag atttttggaa aaaataaaaa
    10501 aatttggaaa atagtttttg caatattgac ggcagtggca gcattgtcat ttatgtacta
    10561 agttatttta agaatgtagg gaaataaacc ctacattctt tttagttttt tctgttttct
    10621 aaattctatt tatccaagcg cttcaacatt tcttgcttct tcgcttcaag ttctgcacgc
    10681 ttttcttcga tttcggcatg ttttttctcg agttcagaac aacttgcacc attgctaaat
    10741 tcttttcgcc atcaggagat agggtgagtc gacatgtcta ttactcaccc aaagcagtcc
    10801 tacaaagcag gaattttctg ttactttttt ggaaatagta acgtttatac agctttgaca
    10861 cttcgtatca aagcgccaaa cacactccga gggatttaca gaaagcagaa aaggaatgat
    10921 ctggtataag atcattcctt ttctctcttt ttctttaagt aattatatac aatgtacgac
    10981 gaagtcgtca ttgcaatgct gatccaccac ctaaaggaaa ctttaaacaa cattgataag
    11041 ataaagaata taaacaacga aaatacgtta tacccaatta attttattgt atatctcatg
    11101 attaaaagtt aatccttccg ttgttaggaa tggcatcatt tttatcccat aattgtgcta
    11161 aataagtccc cggtgataat aaatttatag caaattctaa agcaacatca tttacaaacc
    11221 aactacctag atatctagaa attgctgaac gaatagcact ttttgctgca tgttttcctt
    11281 ttactttaat tagatttgca aggcctgcag tagttcctcc taatgctaaa gctattgcag
    11341 tatctaatag agcacccatt tgattaactg taataccttg ccaagctgct ctaaatggag
    11401 agtatgtagg tgggattgta taatcgcctt gtaattgtcg gttaattact tctttgatcc
    11461 attgttgtga gacgtctgga tgaaaagatt ggatttcgtt tgcaagtgta ttgatttgtt
    11521 cttctgttag agaagtgaca ggttgaagtt ccatatttgt ttcaatttgt gatacttgtt
    11581 cagaagcgta tacagctgaa acacttggaa tcgctgatac aattaacaca attgacgtca
    11641 aaaaaaccga aataaatttc attaatttgt tcatgagctt ttctcctttt tatttgtatc
    11701 tgcttacatt ttatcatatg ctgttattat agtcaaaaaa atatgctatt atgttaaaaa
    11761 aatatttttc aaaatataaa tggacggatt tattttggat tttatttgtt attttgacct
    11821 gcctctatat tggtaaccat gatttgttta ctctcaatca tcaagaattc tcttttcgtg
    11881 gtagcgtttg gggtctggta ctggccttat atcacttact attcattgat aagtttgtta
    11941 tatcgaatcg aaaataaaga ttagagctat gcttgactgt gtacttttag gattgatttt
    12001 ggaggaagat tttgtctcta ttatttatta ttttaaattt atttattttg tataagatct
    12061 attctttgag gcgggaaaaa tcgaaatact tgatttatac ggcctatatc atatttgggg
    12121 taaatgtagt atatggtatt caatggttat taagagaact gatttcaact atttcccctt
    12181 aatgtgtatg gaaactataa caaaacgcat aagattaagg tttgtgtacc tgttcttatg
    12241 cgtttttata atattgaaga ttttcggtaa gttttatacc tttgggaagt ctagtttaga
    12301 atgctttccc aaccaaaatc tctgcttcga tggtaatctc tgtcagttgg ctccagtcaa
    12361 tcttgctctg gtcaacgtga aagaggagag gagtcatact gagtagggct tggagttgtt
    12421 ctgctgtgat agtcttagtc agagaggcag tttgactaga taggatggta aagtgttcct
    12481 ggaaatgctc cttgatatct tggttagaat actccttgtt tgtcagctgg tcctgtactc
    12541 tttgacggat ttctttgagg tgattttcag ttgggataac ctttatcaag ataccgtctt
    12601 tggataaaac gcgacgaaat tctccatagt tggcaggtga gaagatatca agcagaatat
    12661 ccatgttagc gtcttttata ggaagtcgtg ccaagtcgcc aacgaaccaa ttgactgccc
    12721 agttgggttc acttttagca gcgatttgga ctgaatcttt ggagatgtca aaggcataga
    12781 aagttttttc agagtgactt tcttgtagtt tgcgagaata gaatccttca ccacaaccga
    12841 tatccaaaat tgttgtggta gtttttgagc ttgcaagcaa gtcagataca gcatctaaga
    12901 tagcttggta aaagccggct tctaggattt gttgacggtt ttgaaaattt tccttgtcat
    12961 agttagcaga ttgcttgatt tgaggtgcta gattgacata gccaaatttc gccaagtcaa
    13021 aagaatgacg gttgcagcac ttgaaattag tctctaacag agtcagattt tcttgacaga
    13081 taggacaggc aaaggcagtc gcagaagcaa aacgctgaag tttgggcttg agatttgtat
    13141 tcatagttta agtgtatcaa aagaggcaaa tgaaagcaac tttaggaata agtagattca
    13201 gtagaaataa aactaattct ccaatttata gaatgaaatt gcttttatat ctaaattgct
    13261 atataataat gataaggagg aaataagtat gttaaaagaa gtattaaccg ttgcaaaagt
    13321 tgcgaaaaaa tcttcattat ttttgggtgg tgtcgcattt ggtacccttg gtttgaaaat
    13381 cttagcaagt aaggaagcta aaaaaggtta ttctaaagct ttggctaagg cttacaagtt
    13441 gaaagacgag ctagatgcat ctgtttctgt tgtgaagcaa catggagacg atgtcttgca
    13501 agatgccaaa tatttgtacg agcaagagaa aaaagaagag caattagata gccttatagg
    13561 tgaataatat gtcttttaaa gtgctacata gaggatacca acatatccga ctatcatctt
    13621 ctttttcact taccttggat attcaagact atcttcgttc cttggcgaga gatgaaaagg
    13681 ggattgagtc tatccagttt tacatggatc aacagcactt tactctacgc ataaaagaag
    13741 gcttttctgt attagataat gcagaagcct ttttaaaaag aattgataaa gggaaagttt
    13801 ctgagttgat gactcttccc attcgtagag aagagagtgc ttattctatt gtttcaggtg
    13861 cagcggttaa gcgtgtactt tttcgtagtt ttgtgccgta tcctattcgc tatatatgga
    13921 cttgttatca ggctttgggt tatattagag aagcctatca aacactagcg cgtaaggaac
    13981 taacgatgga agtcttggac tgttcggcta ttttattgtc cttgtttatg aaccaatcca
    14041 agacagctag caatatcatg tttatgcttg atttggggaa tcatttagat cagtggtcct
    14101 tgaaaaaaac tgcaacagat ttagaacaga gtcttcttgc aaaagagagc gatgtattcc
    14161 tagtacaggg cgatacggtt gttagtatca agagttccga tgttcaaata ggagatgtct
    14221 tgatcttatc tcaaggaaat gaaattctgt ttgatggaca agtagtttca ggtttaggta
    14281 tggtcaacga aagttccttg acaggagaga gttttccagt tgaaaaaaga gagtctgatt
    14341 tggtttgtgc aaatacagta ttagaaactg gagagttacg cattcgtgta acagataatc
    14401 agatgaacag ccgtatttta cagctgattg agttgatgaa gaaatctgaa gaaaacaaga
    14461 aaacgaaaca acgctatttc atcaagatgg cggataaagt cgtcaaatat aatttcttgg
    14521 gggctgggct gacttaccta ttgacaggtt ctttttctaa ggctatttct ttcctattgg
    14581 tcgatttctc ctgcgctttg aaaatctcta ctcctgtagc ttatttgaca gttatcaagg
    14641 aagggttgaa ccgtgaaatg gtgattaagg atggagatgt tctggagaaa tatctggaag
    14701 ttgatacttt cttgtttgat aagacaggaa caatcacaac tagttatcct atagttgaaa
    14761 aggtgttacc ttttggagac tatagtgagg aagatattct cagaatcagt gcctgtcttg
    14821 aggaacacat ttatcatcct attgctaatg ccatcgtcaa gcaagctgag atagagggaa
    14881 ttgaacatga ggaaatgcat gggaaactcc aatatatcgc aagcaagggg atcaaatctc
    14941 atatagatgg ccaaccagtt cttattggga attatgtctt gatgcaggat gagcagattc
    15001 atatcagttc agaacaaaat gctttaattg aagagtacaa gagtcactac aatctcttat
    15061 tcttggctta tcaaaatgaa ttgattggaa tgttctgcat tcatactcct ttgagaaaag
    15121 aagcaaaaac agccttggat aaacttaagg cacaggggaa aaaattgatt ctggcaacag
    15181 gagacacctt gattaggaca gaggaattag tcaaagattt gccctttgat caggtctata
    15241 cagacttgaa acctgatggg aaatttgagt tagtagagaa actgcagaaa gcaggtcaca
    15301 ctattttgat ggttggagat ggattgaatg actcagcggc tttaactcta tcagatatcg
    15361 gtgtggtgat gaatgagagt gcagatattt ctaagcagat gagtgatatc ttattgttag
    15421 ataatcgttt ggatttcttc caagagttgg attcgttatc atcatctttg caaacactca
    15481 tcaagaaaaa tattcaggat accgttgtcg taaatagtag tttgattggc tttggcttat
    15541 ttaattggtt cagtccttca aatctctcta tcttacataa tctaacaacc ttgcgcattg
    15601 tcctgcgtag cctgtctatt aaaaatagat agatgagagt tattaacagc aaaaaggagt
    15661 tacctttctc gaggttaact cctttgttta tagataaatg ttgaatagtt tagtaagtca
    15721 atacaaagta ttttttcttc ccacgacgga taacagtcag ttcattttct aacttatcag
    15781 cgtcactcaa gacatagtca aggtcttgga tgcggtcgcc gtttacgtag atggctccgt
    15841 tttggacgtc ttcacgggct tggcgttttg agttaactat accagatgag acgagcagtt
    15901 ccacgatatt gttgttttcg tctgcctgta cttggtagtt tggcacacca cgaagtcctt
    15961 gtttgagctc tttgacagaa aggtttttga tgtttcctgc aaagagttgc tcagtgatgt
    16021 taagtgcttc tttgtaggct tcttctccgt gaacaagtgt aacaacttca cgagccaaga
    16081 ctttttgagc caagcgttcg tgtggcgctg cttcaaattg tttacgaata tcttcaatct
    16141 catcaagtga caagaaagta aagattttca agaagcgaac agcgtcagcg tccatcacgt
    16201 tcatccagaa ttggtacatt tcgtatggag aagtcttttc gggattgagc cagacggcat
    16261 ttccttctga tttaccaaat tttttaccag ttgcatctgt gattagtgga acagtgataa
    16321 cgtgaccagt cttgtccgcc ttacgacgaa gcaattcggt accagctgtc atatttcccc
    16381 actggtcaga accaccgatt tgaagagtga cattatggtc ttggttaagg acgaagaagt
    16441 cgtacccttg catgatttgg taagcgaact cagtgtaaga aattcctgtt tcgatccgtt
    16501 ttttaacaga ttccttactc atcatgtagt tgaccgtgaa gtactttcca atgtcacgga
    16561 ggaagtcaat gaagctgatg ctgccaaacc agtcgtagtt gttgaccatg acagccttgt
    16621 tttcgccatt ttcaaagtca agaaaacgag aaagttgtcc ttggatagac ttgacccagc
    16681 catctactgt gtcttttgtt tggagactac gttcagcatc tttgaaggac ggatctccga
    16741 tgagacctgt agcaccgcca acgagcgcat aaggtttgtg acctgctagt tgcaagcgac
    16801 gacttgtcaa gattgcgaca aggtggccta ggtgaaggct gtcagcagtt ggatcgtagc
    16861 cagtataata agaaacttga ccttcttcta gggctttacg caaagcttct tcatcagtcg
    16921 tttgaaatat caaaccacgc tcttttagct catcaaaaat gtgcatgtgt cttttctcct
    16981 ttttaaaatt attgtttcta cctattgtat cacaaacttg ggcaatagcc tagcagaata
    17041 gggaagaaag tggctatttt attgaaaatt tcccttttgt ggtataatag ataaagtgag
    17101 gaaatccatg caaaatcaat taaatgaatt aaaacgaaaa atgctggaat ttttccagca
    17161 aaaacaaaaa aataaaaaat cagctagacc tggcaagaaa ggttcaagta ccaaaaaatc
    17221 taaaacctta gataagtcag ccattttccc agctatttta ctgagtataa aagccttatt
    17281 taacttactc tttgtactcg gttttctagg aggaatgttg ggagttggga ttgctttggg
    17341 gtacggagtg gccttatttg acaaggttcg ggtgcctcag acagaagaat tggtgaatca
    17401 ggtcaaggac atctcttcta tttcagagat tacctattcg gacgggacgg tgattgcttc
    17461 catagagagt gatttgttgc gcacttctat ctcatctgag caaatttcgg aaaatctgaa
    17521 gaaggctatc attgcgacag aagatgaaca ctttaaagaa cataagggtg tagtacccaa
    17581 ggcggtgatt cgtgcgacct tggggaaatt tgtaggtttg ggttcctcta gtgggggttc
    17641 aaccttgacc cagcaactaa ttaaacagca ggttgttggg gatgcgccga ccttggctcg
    17701 taaggcggca gagattgtgg atgctcttgc cttggaacgc gccatgaata aagatgagat
    17761 tttaacgacc tatctcaatg tggctccctt tggccgaaat aataagggac agaatattgc
    17821 aggggctcgg caagcagctg agggaatttt cggtgtagat gccagtcagt tgactgttcc
    17881 tcaagcagca tttttagcag gacttccaca gagtcccatt acttactctc cttatgaaaa
    17941 tactggggag ttgaagagtg atgaagacct agaaattggc ttaagacggg ctaaggcagt
    18001 tctttacagt atgtatcgta caggtgcatt aagcaaagac gagtattctc agtacaagga
    18061 ttatgacctt aaacaggact ttttaccatc gggcacggtt acaggaattt cacgagacta
    18121 tttatacttt acaactttgg cagaagctca agaacgtatg tatgactatc tagctcagag
    18181 agacaatgtc tccgctaagg agttgaaaaa tgaggcaact cagaagtttt atcgagattt
    18241 ggcagccaag gaaattgaaa atggtggtta taagattact actaccatag atcagaaaat
    18301 tcattctgcc atgcaaagtg cggttgctga ttatggctat cttttagacg atggaacagg
    18361 tcgtgtagaa gtagggaatg tcttgatgga taaccaaaca ggtgctattc taggctttgt
    18421 aggtggtcgt aattatcaag aaaatcaaaa taatcatgcc tttgatacaa aacgttcgcc
    18481 agcttctact accaagccct tgctggccta cggtattgct attgaccagg gcttgatggg
    18541 aagtgaaacg attctatcta actatccaac aaactttgct aatggcaatc cgattatgta
    18601 tgctaatagc aagggaacag gaatgatgac cttgggagaa gctctgaact attcatggaa
    18661 tatccctgct tactggacct atcgtatgct ccgtgaaaag ggtgttgatg tcaagggtta
    18721 tatggaaaag atgggttacg agattcctga gtacggtatt gagagcttgc caatgggtgg
    18781 tggtattgaa gtcacagttg cccagcatac caatggctat cagaccttag ctaataatgg
    18841 agtttatcat cagaagcatg tgatttcaaa gattgaagca gcagatggta gagtggtgta
    18901 tgagtatcag gataaaccgg ttcaagtcta ttcaaaagct actgcgacga ttatgcaggg
    18961 attgctacga gaagttctat cctctcgtgt gacaacaacc ttcaagtcta acctgacttc
    19021 tttaaatcct actctggcta atgcagattg gattgggaag actggtacaa ccaaccaaga
    19081 cgaaaatatg tggctcatgc tttcgacacc tagattaacc ctaggtggct ggattgggca
    19141 tgatgataat cattcattgt cacgtagagc aggttattct aataactcta attacatggc
    19201 tcatctggta aatgcgattc agcaagcttc cccaagcatt tgggggaacg agcgctttgc
    19261 tttagatcct agtgtagtga aatcggaagt cttgaaatca acaggtcaaa aaccagggaa
    19321 ggtttctgtt gaaggaaaag aggtagaggt cacaggttcg actgttacca gctattgggc
    19381 taataagtca ggagcgccag cgacaagtta tcgctttgct attggcggaa gtgatgcgga
    19441 ttatcagaat gcttggtcta gtattgtggg gagtctacca actccatcca gctccagcag
    19501 ttcaagtagt agttctagcg atagcagtaa ctcaagtact acacgacctt cttcttcaag
    19561 ggcgagacga taattctcta aatgaagtgg ccaatcaatg gattgccact tttttccttt
    19621 cgtgttacaa taagggtatg aatcagtatc agaaaaagat tgttaacgga aaaatttatt
    19681 cgctcctatc cggcttaata tggggaatct gtggaatttt aggagagtac ttctttactc
    19741 attatcaggt gtcttcgggc tggattacct ctatgcgttt gacactggca gggagtcttg
    19801 tactcatttg gtctgcaata caattaaaat cgcaagtgct agatatttgg cgagacaaga
    19861 aaaattacct gcccttttta gcctatgcta ttttggggat tttttcagtt cagtattttt
    19921 tctatctctg tttagaatac tcaaatgcta cgacagcaac tattttacag tttattagcc
    19981 ctgtctttat cctcttttac aatcgcttgg tttatcaaaa acgagcgtca aaaagcgctg
    20041 ttttctatgt tttggttgcc atgctgggtg tttgcttgat ggcgacaaag ggagatctct
    20101 ctcagttatc catgacgccg ctagctctta taacaggttt gctgagtgcc atgggtgtta
    20161 tgtttaatgt tatcttgccc caaccttttg ctaagcgtta tggttttgtt cctacggttg
    20221 ggtgggggat gattttggca ggtttgttta gcaatgtcct ctcgccggtt tatcagcttt
    20281 cctttactct tgatatttgg agtatcttga tttgcctcat tatcgctttc tttggaacgg
    20341 cttttgcttt tttcatttcc atgaaggctg tgtccttggt ttctcctttg gtggtttccg
    20401 ttatcagtgc cagtgaacct ctctcttctg ctctcttgag tgttttgttc ttaggattag
    20461 tagtggattg gtccctcctt ctagctatag ccttgattat tttacccatg atatttttgt
    20521 ctatagaaga agcgaaagaa agtagataaa aagtcttttc ttaattctaa aagtgtgcta
    20581 tactagaata gtcaataaaa cacggggaga tagttgtgaa gagtattttt aggttgtatc
    20641 ggtaggggct tgcttttacc gacagcacgt tttatctcac atccctaaaa aatcatacct
    20701 aaaaacagac aaaaaggttt caaatttgag gccttttttg gtagaatagg tatcattata
    20761 acgaaccagg aggcacctat gactgctaca aaaatgaacg ctcaagaaat tatccaattt
    20821 atcgccaatg ctgaaaagaa aaccagtgtc aaagtaacct ttgaggggca actcgcaact
    20881 gctgtgccta gctctgttgt caaactagga aatgtcctat tcggagactg gaaggacgtg
    20941 gctccgcttc ttgaaggttt ggtagaaaat caagattatg ttgtcgagca agatgctcgt
    21001 aattctgcag ttcctttgct agataagcgt gctatcaacg ctcgtatcga gccaggtgct
    21061 attatccgtg accaggtgga aattggtgac aatgctgtta tcatgatggg agctgttatc
    21121 aatatcggtg ctgaaatcgg tgctggaacc atgattgaca tgggtgccat ccttggtggc
    21181 cgtgctatcg ttggaaaaaa tagccacgtt ggtgcaggtg cagttttggc aggtgtgatt
    21241 gagccagcta gtgctgaacc agtctgtgtc ggagacaatg ttcttatcgg ggctaatgca
    21301 gtggttatcg aaggagtcca aatcggtagt ggttcagttg tcgctgcagg agctatcgtt
    21361 acccaagatg tcccagaaaa cgtggtagta gcaggtgttc cagctcgtat tatcaaagaa
    21421 attgatgccc aaactcaaca aaaaacagcg ctagaggatg cgcttcgtac cttgtaattg
    21481 taaaagtaaa aaagaggcgg aacccttttt ccagcctctt tctgctatat cggaggacag
    21541 atagatgtta gatttgattc agactagacg agctttacac cagattccag agattggctt
    21601 ggaggagttc aagactcagg cttatttgct ggatgtgatt gagaaattga ctacgggcaa
    21661 ggattttgtt caaattcgta cttggcggac agggattttg gtctacctgc agggaagtca
    21721 gccggagcga accattggtt ggcggacaga tattgatggc ctgcctatcg tcgaacaaac
    21781 aggtctacct tttacttctc aacaccaagg tcgcatgcat gcctgtggtc atgattttca
    21841 tatgaccatt gccttaggct gtcttgagcg cgcccttgag gagcaaccca agaataatct
    21901 gctctttcta tttcagcctg ctgaagaaaa tgaagctggt ggtatgctca tgtatgagga
    21961 cggtgctttt ggagattggt tgccaaacca attctatggt ctccatgttc gtccagattt
    22021 gaaagtcggc cagattgcga ctaatactca tacactcttt gcaggaactt gcgaggtgaa
    22081 gatccgtttc aaaggtaaag gtggccacgc agcttttcct catgaagcca atgacgcctt
    22141 ggtggcggct agttactttg tgacccaagt gcagtcagtt gttagccgca atgtcaaccc
    22201 aatcgaggga gcagtggtga ccttcggcgt ttttcaagct ggaaccacca acaatgtcat
    22261 cacagacaca gcttttttac atggaactat tcgtgccttg acacaggaca tgagtctctt
    22321 ggtgcaaaag agggtaaaaa cagttgcaga aggggttgca gctgcctttg atatggaagt
    22381 cgaagtggaa ctcaaacaag gtggatacct acctgttgag aacaatccag ccttggcgcg
    22441 tgaactgatg gacttctttg acgagaaaga cggaatcgag ttgattgata tcgaacctgc
    22501 tatgacaggc gaggactttg gttatctcct ttcaaaggtt gatggcgtga tgttttggct
    22561 aggtatcgat agtccctacg ctcttcatca ccctcaaatg agtcctaagg aagaagtctt
    22621 agctattggg gtggctgcgg tgtctagttt cctgaaaaag aaggcagcag aatagagggc
    22681 ttgtctatga aatcggaatt acgcaagcaa gtcttgcatg aaatgaaggc tatatctcaa
    22741 gagcaaaaac aggctataga ccaagcttta accgagcgaa ttttacaaca ccccttttat
    22801 caagaagcca aggtcatcgc aacctacctc tctttttctc atgagtttca aacgcgggaa
    22861 ctgattgagc aggcgctgaa ggacggcaag aaggttttga cacctaagac ctatcccaag
    22921 gggcgcatgg attttgtggt ctatgatccg caacagttgg taaaaacttc ctttgcctta
    22981 ctggagccac agggagattt ggaagtagtg gatgcatctc agattgattt gattcatgtt
    23041 cctggcctgg cttttacgac ggatggatat cggattggat atggtggagg ttattatgac
    23101 cgctatctgg aacatttttc tggtcgtgct ttgagtacga tatatccttg tcaaattcag
    23161 gactttatac ctgaaaacca tgatattcct gttcaggagg tattaattga tgaaggaaat
    23221 ctttgataga cgttaccctg tgacgagttt cttcctctta gtgacggcct tggtattttt
    23281 actaatgttg gtcactgcag gcggaaactt tgacagggca gatacattat ttcgatttgg
    23341 agccatgtat gggccagcta ttcgcctctt tcccgagcag gtttggcgtc tcttgtctgc
    23401 catttttgtt catattgggt gggaacattt cattgttaat atgctttcac tttattatct
    23461 tggaaggcag gtagaggaga ttttcggttc taagcagttt ttctttctct atcttttatc
    23521 aggaatgatg ggcaatctct ttgtttttgt atttagtcct aaatccttag cagcaggcgc
    23581 ctctacctct ctttatgggc tatttgccgc gattattgtt cttcgctatg caactcgcaa
    23641 tccttatatt caacagctag ggcaatccta tctgacactt tttgtagtta acattattgg
    23701 aagtgttctg attccaggaa tcagcctagc aggccatatc ggtggtgcag ttggtggcgc
    23761 atttctagca gttatctttc cagttagagg agaaaaacgg atgtataata ccagccagag
    23821 attaggagcg gtagtcttgt tcgtaggact cgccattttg cttttctaca agggaatggg
    23881 aatgtgacga agttgaaaga aaatttgtgt aatgaaaaaa gataaggcat tttttgaacc
    23941 ttatcttttt attttatttt attccttctc agtcaattct tttccaagtt ggatgaggta
    24001 attcttcaaa tcatctttga cttgtgggtg tttgagggcg tagtcgatgg atgttttcat
    24061 gaagccaaac ttgtctccga catcgtaacg agccccattg aactcacgag caaatacacg
    24121 ttgtgtttta ttgagggtgt cgattgcatc tgtcagctga atttcatttc ctgcacctgg
    24181 agcttggttt tcgaggattt ggaaaatttc aggcgtgagg aggtagcgtc cgataatagc
    24241 aaggtcgcta ggagcgtcct ctggagctgg tttttcaaca aaggtttcaa cactgtaaag
    24301 accatctttt ccttcgcctt gcggagcaat aaccccgtaa gcagatactt cgtcatgagg
    24361 gactggcatg acagcgatag tagacgcgtg ggtacgctcg tagtcatcca tgagttgttt
    24421 ggtaagtgga acagcctttt cgtctgtgat atccatcaag tcatcaccaa gcataacgac
    24481 aaaaggttca tttccgacga aagccttagc ttgcaaaaca gcatctccga gaccgcgtgg
    24541 atgagtttgg cggataaaat gcagacgcat gtcagttgtt ttatcaacta gcttcaaaag
    24601 atctgttttc cctttttctt tgaggttata ttccaattcg aagtttgaat caaagtggtc
    24661 ctcaatagaa cgttttgatt taccagtgac aactagaata tcttcaatac ctgatttgag
    24721 agcctcttcc acgataaact ggatagttgg tttgtctacg attggcaaca tttctttggc
    24781 aagggccttg gttgctggta aaaatcgagt tcctagtcca gcagcaggga tgactgcctt
    24841 tctaactttt gatgtcataa aaatcctttc tatagagggt taagaccact cattttctgc
    24901 tttaaattca ttgttcatga tgtcataaat ggcatctttg atattggttc cgtgataaat
    24961 aacttggtaa atagcctgtg taatgggcat atagactcca agttcttggg ctagttcata
    25021 ggctgctcga gtcgttgaaa ttccttcgat taccatgccc atattagctt ctatatcagc
    25081 tagggattct cctcgtccga gagcatctcc agctctccag ttacgagagt ggatggaagt
    25141 tcccgttacg atcaaatctc ccacaccaga taagccgcta taggtcaatg gactggcccc
    25201 gagtgctacc cctaggcggg tgatttctgc taaacctcga gcgatgatgg ctgccttagc
    25261 attatcacca aatccaagac catgtaaagc tccagcaccg acagcaataa tatttttaag
    25321 agcaccagca gtttcaaccc cgataacatc cgtattggta taaagtcgga agtagtgatt
    25381 actaaatagc tcctgaacgt attgagctgt ttgtaaatct ttagaagcag cagtgattaa
    25441 agttaggtca cgcacaatgg tttcttccgc atgactaggc cctgaaacaa cgacaatatc
    25501 actgcggagc tgttcaggaa tttcttcttc aaggatagtt gataatcgtt tatggctatc
    25561 aggttctaat ccctttgaag cgtgcatgat gatagcctta tggtccaagg tttgtgcaac
    25621 ttgttgggca acaagtcgtg tcacttttgt tgggacaaca aacaaaatcg catccacatc
    25681 tttcaatgtt tctgctaagt cggtgtaggc aatgatattt tcatctagaa cgacatcttt
    25741 aaagtagtgc ttgttagtat gataggtatt aatttcattg atttgctcgg gaagatttcc
    25801 ccaaatacgt acctcgtgtc cattgtcatt taagacttgt gaaagggcag ttccccaaga
    25861 accaggcccc aagacggcga cggtttgttt ttccatgatt tcctccttga tagagttctt
    25921 tccattattc tatcatattc tagaggattt tgcacttgca ttactggggt atggtggaaa
    25981 aatacacaaa gaccgagatg aagggataaa ctcttagtct ttagttaaag tgagaagaaa
    26041 tctgatagat agtttgattc agtatatgct ataaatagag gtaaacaact caggaggttc
    26101 ttgtaggttg cgagagtttg gcgaaaaaat taaaagatta cgtttggcta aaaaaatcag
    26161 tcgttcagaa ttttgtggtg atgagtctga attaagtatc cgtcaattaa ttagaattga
    26221 aaatggagaa tccagaccaa cactaacaaa gttaaaatat atcgctgaac gtttgggggg
    26281 ggtgaagatt acaagttgat gccaagttat atagagttgg ataaggaata cctagaattg
    26341 aagtatttct tgatgaggac tcctacatac gaagatgaaa ctatcgccca aaagaaagag
    26401 agtgtttttg ctaagatttt tgaagagtat tatgataggc tacctgagga agaaagattt
    26461 atcatcccaa attattcata tctagcacta gcgaactaca cagttcaaaa attaccagaa
    26521 aagctagttg aaatactgtc cttctggtga ttttgcttaa tttctgatgt tcgagacatg
    26581 attgaggaga tttgtcttga ttttttccta aaaaagggca gactcctccc ttggtttgat
    26641 gctcgatttt taaaataagg tgacttattc tgttaggcat gttttcccct gccctctagc
    26701 tctatatgga acagggagta tcagatttat ctggcagatt gtatcaaata aggcttgaaa
    26761 ctgttttgaa taggcgtata gacttgagaa tttgagaata tgtcgtctag cagtagagac
    26821 atattttccg gcaatatgaa ggaagagacg tcgaaaacgc ttgatagtca aggactttac
    26881 ttcatcacca gctagctgct ttaaaaagag gtagagatta taagccagac agcccatcat
    26941 catacgtact tcattcttaa tcatggtcga actatctgtc ttatccccaa agaatcctgc
    27001 tttcctttct ttgataaagt tttcagcatt ccctcgttca cgatagaatt gaaacagctc
    27061 ctcactatct acactgctta aattgttcaa atgaactttt ttacaaccat aaaaaagagc
    27121 acttaatcgt actcttctct tttaaggatt agttgaatta attcaaatcc actagttctc
    27181 ctgtttctag gtagactagg cgttcacaaa tgttagcaat gtaatcagcg aagcgttcca
    27241 gatgtcctat gatataaagg tattgagttc cattgggaat tgaggtttct tggtctttca
    27301 taagtccaat gatttccttt gataaggcat aataatattg gtcaatctgt tcatcttttt
    27361 gagcaatact aatagctttt gaggcttggt gcaaaggaaa ggcaaccaat aaatcggcta
    27421 gcatgctgag ggataattta cccatttggt gtaactgttc ttcgtcaggg gctagttgat
    27481 tttcttttag ttgcaaaaca gctttggcaa tgcctgccat atggtctccc atacgttcaa
    27541 ggtctgaaca agaagacatg atgctaatca caaatcgaag gtcagacact tgtggctgtt
    27601 gcaaggccaa caaacgggca caggtcaatt cgatagcgct ttgaccttgg ttgatagcat
    27661 gatccttatt gataattagc tctgccatct ccttgtcttt ggaggctaag gccagtaagg
    27721 cttttgaagc tgtttcaagg acaagttgcc ctagtcctaa aaaggattgt tctaattcat
    27781 gcaattctaa gtcaaattga tttctcatag gtttttcctt ccttatccga accgtcctga
    27841 aatatagtct tctgtgcgct gatcttttgg attggtaaac acgtcaacgg tatctccaaa
    27901 ttcgcaaatt tctcctgtta agaaaaaagc agttttatct gaaatacgtg aagcttgttg
    27961 catgttatgg gtaacaatga taatcgtata atcctttttt agttgctgaa tgaggtcttc
    28021 aatttttaaa gtggagatag ggtctaaggc tgaagtcggc tcatccatta acagaacatc
    28081 aggttctact gctaaagctc gcgcaatgca aaggcgttgc tgctgaccgc cagataagga
    28141 catggcactc tttttaagat catctttgac ttcttcccaa atggctgccc cttttaaaga
    28201 tttctccact aaggcatcta attgtttttt gtctcgaata ccatgtgtcc ttggaccata
    28261 agccacgtta tcatagatag acatggcaaa gggattaggc tgttgaaaaa ccatccctac
    28321 acgcttgcgt agctgattaa ggttgaattt gctactataa atatcttgct catctaagag
    28381 gacttggcct tcaatatggc aagaaggaac caaatcgttc atccggttaa gggtttttag
    28441 aaaagttgat ttgccacaac cagatgggcc tatcaaggca gtaatctgtc tttctggtaa
    28501 ttgaatcgaa atatttttta aggcttgaaa atccccgtaa aataagtcta ggtgtctgac
    28561 tgaaaatgtt cccatactag gaagctcctt tcacaagttt tcgagataat aagcttgata
    28621 gagtatttat cattaaaaca gtaataatca aaatcacacc ggtagcatag gcttcattga
    28681 catgtagccc ctcacttgac agcatataca tatgcagggc tagagaacga cctgaagaca
    28741 tgagactact tggcgtattg gtagaggtac ctaatgtata catgagggca gctgtctcac
    28801 caacgatacg gccaatagct agtatcactc cagctaaaat acctggcatg gcaactggta
    28861 gaacaattct aaaaacagtc cgtaacttac ctgccccaag tccataactt gcttgacgca
    28921 tgctatcact aacagataaa agggcttctt ctgttgagcg aataatgact ggcaacacca
    28981 tgatgactga ggttaagatt cctgataaca gagagtattg aaaacctaag aagactacaa
    29041 agaagagcat gccaaacaga ccaaaaacaa tggaaggaat cccagacaag gtatctgagg
    29101 ccaatcgcat gattttaaca caaagggaat ctttttttgt atattccaca agataaaaac
    29161 cagcaaaaat ccctatgggc aaggctaaaa gaagagcacc aaagaccaga ataacggtgg
    29221 aaataatcgc tggcataagg gaaatgttct cagaagtata agtccaagaa aagagggata
    29281 gacttagatg aggtaagcct ttgatgagga taaaaccaat gattaaaaag agagagccaa
    29341 aggttaaagc tgaaaaacaa taaacgagaa gttttagcag gtatttactc ataagatgat
    29401 tttcctttca agtaggcaaa gtaggcatta atcaagagaa taaggaaaaa gagaactgct
    29461 gaggttgcaa taagggcttc cctatgctga cctgatgcgt aagccatttc cagaacaata
    29521 ttggttgtta aggttctggt tcctgaaaag agtccacttg gaataatcgg ctggttgcct
    29581 gccaccaaaa taactgccat ggtttcacct actgcgcgac cgattcctaa aataactgct
    29641 gataaaatac cagatctcgc agctggcaag atgacactaa aaatactccg ttcatgacta
    29701 gctcctagag ccaagctacc agaataatac gttttgggaa ctgttcggat agcagattct
    29761 gacaaactga taatggttgg caaaatcatt attcctaata gtaacgaagc ggttaggaca
    29821 ctcatgccat ttcctaaaaa ggttctaatc caaggcacca ataattgtag gccgaaaaaa
    29881 ccataaacaa tagatggaat ggccgccatc aagttgatag ctgattttaa gaagccatag
    29941 acgggctttg gacaataata aaccataaac accgatgtca agatgcctgt tggcacccca
    30001 atcacaatcg ctcctaaggt aattaataag gaaccaacga tcattggtaa aataccatag
    30061 cttgccggaa tgttcgttgg cgaccaatca ctgcctaata aaaaacgggc aaagccgtag
    30121 ttagccatga aaggtaagcc attactaaaa ataaagaaac agattagcaa aatagctaca
    30181 acagctactg ttgcactcat gaaaaaaatt gccctaaaaa ctgcttcttt gaaggcttgt
    30241 tttgtcacat cttgtccttt ctagtgaaga aagtaaggga gatacgacac ctccctactt
    30301 gccttcttta tcttattgta cgatgaaacg tctgcatctc tttagagatt tatggagcaa
    30361 acattttatt taatcttgtc ccaggtggtt aatttgccac taaaaacgtc tgcaagttca
    30421 gccatactga cttggcttgc cttattgtca ttattgacca caacagcaat accgtctaaa
    30481 gcaatagcat catgggtgag actcttacct tcttcaggag ttaattccct agaaaccata
    30541 ccaatatcag cggttttctc cttaacagcg gtaatacctg ctgaagaccc attagaggta
    30601 atatcaatcg taacttctgg attttctttt ttataagctt ctgctaattt ttccattaaa
    30661 gaagatactg aagtggaacc tacaacagac aacttgcctg ataagtgttg gcttgtatat
    30721 tctgtggttt cggttttagc ttcaataaat ttattatctg tgaccacttg ttgaccttgt
    30781 ttggagtgga taaagctgat aaaatcttga cctagcttgg aaagattaga agaccaaaca
    30841 atgttgaagg gacgttgaag agggtattca ccatctaaaa ctgtgtctcg actagccttg
    30901 acaccatcaa tctctaaagc cttgacagat ttcgttaaag atcccaagga gatgtagccg
    30961 atagcattag cattcccttg aactgctgag agaacacctt ctgtactatt ttgaatcaca
    31021 gctgttttgg cagtgttgtc aattttttta tcaccgtctt ttttgagaat ccctgtgatt
    31081 tctgtgaagg caccccgtgt tccagagcca ttttctcgtg aaatcacctc aatcgttcct
    31141 gaagctgact gtttggaagc agctgactga ttgccacagg caacaagccc aaatcctgat
    31201 aagccaatgg ctgcaagagt aagcattttt ttgaatttca taataatcac ctttatctct
    31261 atgtattttt cttgtgtagg cttactacat ttatagtcta acaagtcttt gtaaaggttt
    31321 atccctgatt catgtaaaga ttgtgtaaag aatcaaaaaa agccactttt gaaaaatggc
    31381 tgctcctaaa aatagcttta aaaattatta gtcctgtgcg aaagattggt taggaagaaa
    31441 aatcgtgaag caactgcctc tgccaagctg actcgtcacc gtgacttggc cacctaataa
    31501 ttgactgagt tctttgacaa tggcaaggcc aagaccagtg ccaccagttt gtctgcttcg
    31561 acctttatta actcggtaaa aacgttcaaa aatacgatcc tgctctaatt gactaatacc
    31621 aatccctgta tctgatacag aaatcttaat gccttcgttc accttttggg tcttgacctc
    31681 aatttttccc ccttgttcag tgtaacggat ggcattggat aaaagattga gtaagatttg
    31741 ggaaagtaat tgactatctg atacgagggt gacatcatct ggcacctgca cctttagctg
    31801 taaatccttc ttcttgagct gaggttgcaa gctttgagtc aaatcctgta caaattctgc
    31861 caaagaaagg gtcgtccatt gtataggcat ttgttgagcc ttagataagg taagaagatg
    31921 ctcaacaata tgctcaagac gcaagctttc tttgtaaata atgtctagaa agtcatcctt
    31981 gagcgcttct tcttcagctg acatcccctt aatggtttca gcaaagccct taatcgaagt
    32041 aactggtgtc ctcaattcat gggaggcatt tgagacaaag gctaaattta acttttcata
    32101 agttctaatc gttgttaaat catatagcaa gacgagcaca gcttccacag attgggtggg
    32161 gctaaaaacg ggaactgctg tcacttctaa aatcaagtca ccctcatgaa acccacttac
    32221 ttcttgtttt aaccttgttt tttgatcaaa ggcttggtga actaaatttc gaatatccat
    32281 ccgtttgagg tcatcaagtg aacttatgtc gccgtccaca tcgggaaaat aatgaggcag
    32341 agagcgactg gataataaca tctgaccttg agcggaaact aaaaacgtcc ccatggttag
    32401 gtgcgacaga agaacctcca ttgtttcggc taggtccttg tattgctgat cctgttggga
    32461 gactttggtt tttagggcag acacatactg agccaaagac tttaagtctt cttgcccttt
    32521 ttctaaaaag tattcactac tggtcaagag aggttggtgc aaggtctcaa aagcaacttc
    32581 ccatttccaa aggcaaaaga gccagtagcc accttgtccc aaagaaaggg ctagaagaaa
    32641 gagaccgatg cctttactga tccaagttaa tgccatccct gcaatcagaa tgaggctaac
    32701 acttagattg actagccaaa attgaaggta gcgtttcatc tataactcct tgaacttata
    32761 accataaccc cgaatggttc gaataaattg aggggcttta ggattgtctt caattttttc
    32821 cctcaactta ccaatatgaa cgtccaccaa acgtgtttcc tgcccaaagt cataccccca
    32881 gatacgttcc aaaagacgct ctctagtcag tgtcatgttg ggatgtttca taagatagag
    32941 caagagttca aattcttttg gggtcaaact cagtaactta ttcgccttgt agacttcatg
    33001 acgctcaggg tatactttca aggtcccaaa tagccaagaa tcgtcagcga tattatctga
    33061 atcatctcct tcttgttctc ctttagttcg cctgaggaca gccttgacac gcgccagcaa
    33121 ttctctaggg ctaaaaggct tggtcaggta gtcatcagcc cctaattcca aggccaaaac
    33181 cttatcaaat tcatcacttt tcgcagaaac catcataatt ggagttttga cgcctttggc
    33241 tctcagccgc ttacaaactt ccatgccatc taattgtggt aacatgatat caagcaagat
    33301 aaaatcaaag ggttctgttt ctgccaaagc taaggccttc cgtccatttg ttaccaattg
    33361 agtagaaaag ccttccttac ttaaatggta gtcaagcaat ttcagaatgt gttcttcatc
    33421 atccactaat aagacttgtt ttgtcatcta ttatctccta ttggtaacat tataacacaa
    33481 ttatcagaaa tcctaacatt gctaaatcag attaaatttg cttatcaaga ctagtatctg
    33541 gtcaaacgct caatcatctc cttgtgctct ggataggtcg ccagtagatc taccctttca
    33601 aataattcaa aatcctcaaa ttcaaaacca ggagcaacaa gacaagaaac cagagcataa
    33661 tccttatcaa ctgttgatcc ccaaatagtg cccttaggaa cacagtagtg aagttgttgc
    33721 cctttggata tgtccaggcc taaagtgact gcttcgtagt gaccatctgc tgtaatcatg
    33781 tgaacagtaa gtggggatcc tgcatgaaaa taccagattt catctgctgt caatcggtga
    33841 aaatgtgaag gattcgtttc ttctaataag aaataaatac tggtataaag cgcccttccc
    33901 ttaccagcaa ggtttatagt gtctgaagct ttttttgttt gtctaaaata gccaccttca
    33961 acatggggag ctaactctag agttcttatc aagtcttctt t
//
LOCUS       DAVBHS010000005        33898 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVBHS010000005 DAVBHS010000000
VERSION     DAVBHS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33898
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.005"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(108..1382)
                     /gene="psr"
                     /locus_tag="VQ651_000149"
     CDS             complement(108..1382)
                     /gene="psr"
                     /locus_tag="VQ651_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HET2753204.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            complement(1379..2227)
                     /gene="pheA"
                     /locus_tag="VQ651_000150"
     CDS             complement(1379..2227)
                     /gene="pheA"
                     /locus_tag="VQ651_000150"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET2753205.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYIDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            complement(2224..2700)
                     /locus_tag="VQ651_000151"
     CDS             complement(2224..2700)
                     /locus_tag="VQ651_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET2753206.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRHVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            complement(2693..3976)
                     /gene="aroA"
                     /locus_tag="VQ651_000152"
     CDS             complement(2693..3976)
                     /gene="aroA"
                     /locus_tag="VQ651_000152"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET2753207.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            complement(4068..4406)
                     /locus_tag="VQ651_000153"
     CDS             complement(4068..4406)
                     /locus_tag="VQ651_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HET2753208.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            complement(4417..5520)
                     /locus_tag="VQ651_000154"
     CDS             complement(4417..5520)
                     /locus_tag="VQ651_000154"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HET2753209.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            complement(5530..6696)
                     /gene="aroC"
                     /locus_tag="VQ651_000155"
     CDS             complement(5530..6696)
                     /gene="aroC"
                     /locus_tag="VQ651_000155"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET2753210.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            complement(6706..7773)
                     /locus_tag="VQ651_000156"
     CDS             complement(6706..7773)
                     /locus_tag="VQ651_000156"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET2753211.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            complement(7792..8646)
                     /locus_tag="VQ651_000157"
     CDS             complement(7792..8646)
                     /locus_tag="VQ651_000157"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET2753212.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKKMPTEEIW
                     QSLTEKYQ"
     gene            complement(8636..9313)
                     /locus_tag="VQ651_000158"
     CDS             complement(8636..9313)
                     /locus_tag="VQ651_000158"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HET2753213.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            complement(9310..10473)
                     /locus_tag="VQ651_000159"
     CDS             complement(9310..10473)
                     /locus_tag="VQ651_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET2753214.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDERRFIVMDVFEYF
                     KYAKRKNLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            complement(11158..12792)
                     /locus_tag="VQ651_000160"
                     /pseudo
     CDS             complement(11158..12792)
                     /locus_tag="VQ651_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12773..13522)
                     /locus_tag="VQ651_000161"
     CDS             complement(12773..13522)
                     /locus_tag="VQ651_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2753215.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSAPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVEN
                     LRKDHPDQFLESIYLSLAGRKEEVADASQGH"
     gene            13701..15155
                     /locus_tag="VQ651_000162"
     CDS             13701..15155
                     /locus_tag="VQ651_000162"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HET2753216.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKEQGIQPMADVVL
                     NHKAAADNREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(15245..17863)
                     /gene="alaS"
                     /locus_tag="VQ651_000163"
     CDS             complement(15245..17863)
                     /gene="alaS"
                     /locus_tag="VQ651_000163"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET2753217.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLISPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(17885..18370)
                     /locus_tag="VQ651_000164"
     CDS             complement(17885..18370)
                     /locus_tag="VQ651_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HET2753218.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLLDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(18645..18884)
                     /locus_tag="VQ651_000165"
     CDS             complement(18645..18884)
                     /locus_tag="VQ651_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753219.1"
                     /translation="MKMSTFFNKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILEK"
     gene            complement(19137..20207)
                     /locus_tag="VQ651_000166"
     CDS             complement(19137..20207)
                     /locus_tag="VQ651_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2753220.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDS
                     AYAFINFMLKPENALQNAEYVGYSTPNIPAKELLPEETKEDKAFYPDVETMKHLEIYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(20204..20977)
                     /locus_tag="VQ651_000167"
     CDS             complement(20204..20977)
                     /locus_tag="VQ651_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2753221.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(20974..21780)
                     /locus_tag="VQ651_000168"
     CDS             complement(20974..21780)
                     /locus_tag="VQ651_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2753222.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(21761..22918)
                     /locus_tag="VQ651_000169"
     CDS             complement(21761..22918)
                     /locus_tag="VQ651_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2753223.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(22977..23927)
                     /gene="murB"
                     /locus_tag="VQ651_000170"
     CDS             complement(22977..23927)
                     /gene="murB"
                     /locus_tag="VQ651_000170"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET2753224.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            complement(24039..24791)
                     /locus_tag="VQ651_000171"
     CDS             complement(24039..24791)
                     /locus_tag="VQ651_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HET2753225.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWFEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLTAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(24801..25532)
                     /gene="budA"
                     /locus_tag="VQ651_000172"
     CDS             complement(24801..25532)
                     /gene="budA"
                     /locus_tag="VQ651_000172"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HET2753226.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFLHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            25674..26945
                     /locus_tag="VQ651_000173"
     CDS             25674..26945
                     /locus_tag="VQ651_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET2753227.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(26965..27780)
                     /locus_tag="VQ651_000174"
     CDS             complement(26965..27780)
                     /locus_tag="VQ651_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2753228.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEILSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(27937..28590)
                     /gene="phoU"
                     /locus_tag="VQ651_000175"
     CDS             complement(27937..28590)
                     /gene="phoU"
                     /locus_tag="VQ651_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET2753229.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(28602..29360)
                     /gene="pstB"
                     /locus_tag="VQ651_000176"
     CDS             complement(28602..29360)
                     /gene="pstB"
                     /locus_tag="VQ651_000176"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2753230.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(29373..30176)
                     /gene="pstB"
                     /locus_tag="VQ651_000177"
     CDS             complement(29373..30176)
                     /gene="pstB"
                     /locus_tag="VQ651_000177"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2753231.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(30187..31071)
                     /gene="pstA"
                     /locus_tag="VQ651_000178"
     CDS             complement(30187..31071)
                     /gene="pstA"
                     /locus_tag="VQ651_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET2753232.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(31061..31978)
                     /gene="pstC"
                     /locus_tag="VQ651_000179"
     CDS             complement(31061..31978)
                     /gene="pstC"
                     /locus_tag="VQ651_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET2753233.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(31978..32856)
                     /gene="pstS"
                     /locus_tag="VQ651_000180"
     CDS             complement(31978..32856)
                     /gene="pstS"
                     /locus_tag="VQ651_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET2753234.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(33430..>33898)
                     /locus_tag="VQ651_000181"
     CDS             complement(33430..>33898)
                     /locus_tag="VQ651_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HET2753235.1"
                     /translation="QFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQI
                     FGDQLYLLPELLPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQ
                     EEFVKYAAGETVQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
BASE COUNT         9977 a         7810 c         6560 g         9551 t
ORIGIN      
        1 gtataggtaa ctgacttcgt cagtcttatc tacaacctca aaactgtgtt tttagcagcc
       61 tgcggctagc ttcctagttt gcactttaat tttcattgag tattatctta tctcaagccc
      121 atttgagcga gcttggtttg atatttgttt tgatcaacca gcaagcccaa gcctccataa
      181 acatcatagg catctaccca gtcacccagt tctggaatcg tcaatttttc aataccattt
      241 tttgctccat ccaaaacaga taaaccgttt gttaggagga aagtataggg tacgttggtt
      301 gaggtcatag caaaaacctt tccaagagct tcagaaccag tgaaaagttt agtgggatct
      361 ttaatttgct ctaaaattgc tgttaaaact tgttgctgtc tttttgtacg gccgtaatcc
      421 gcctcatcat catcacggaa acgagcataa ttgagcaggg tcgagccatt catctgctgt
      481 tttccgactt taatggtttg ggttggagac tcagtctcgg tagcgtataa atcatctccg
      541 actgtagctt ctgttagggg acgcccattc aatgttgaaa attgagcatc aatcgtcacc
      601 ccatcaggga aaagcgtgtc aatcgctgtg gcaaaggcct ggaaatcaac caaggcgtag
      661 tacttaatgt ccaagtcaaa attatctttc aagacttggc gaaccatttc tgcccctttt
      721 tgcccctctt gttctcctaa ctcgtaggct acgtttaact tgttatctgt ctgttttcta
      781 ccattaatca cttgactata accatctata tagaccaaat tatcacgcat gaaactgact
      841 agcttcattt tcttatctga gcccccgaca tttaatacca taatagagtc agttcgtgtc
      901 tcaacactgt tctggccgat tcgaccatca gtacccatga ttaaaatatt aactccatct
      961 ctagtgtcct gaccattaaa gacttctact tgagctgccc gggcatcagc agttttcttt
     1021 gcgctagcat cttggtaacc acgcaaaaac atgaatacca tggccaaagc cacacagacc
     1081 aaaagtgaaa aaatcaccat aaaaattcgt ttaagacgga gcttccgtct tttctttttt
     1141 ggagggaaag agagtgcttg tgatttggat tgtgagcgac tccggttcgc atagcttggt
     1201 aagtcaacct gctcttctct ttcttgttcc aagctagagc tactatttcc cctagcaaga
     1261 gttagctttt cttgcaaata ggcaaactca tttttttctc tctcattgag atagtgaata
     1321 ttttttagca aataatcata acgcaactgc tcatgatgac ttaagggatt ttctttactc
     1381 atcttctctc ctttccatgg tctgatattg gataaatagg ataggcaccc agaattttat
     1441 actggattcc aatcgcttct aattcttttt gggcaaagtg gaccaagtcc ttatcgatat
     1501 aatccacatc gataatgaaa aagtattcac ccagtgctgt cttgagtgga cgactttcaa
     1561 tttttgtcaa gtcaattcct cgccaagcaa aggtcgacag ggccttataa agtgcacctg
     1621 gaaggttgtc aggtaatgtc aaggccaaac tcatcttttc agtttgtgct tgcaagggaa
     1681 tactaggctt ttcagctcct agaacccaga aacgtgtgaa attggcttcc atttcctgaa
     1741 tatcctcggc aatcagttcc aatccatatt cttcagcaga acttctaggt gcaactgctg
     1801 caaagggctg gtctggatgt tcggaaataa aacgggccgc ataagctgta ctagctgtta
     1861 cctcgatttg agcctctgga tattgttcat cgatgaattt ctttccttga gccaaggcct
     1921 gtggatgtga aaaaatcttt tcaatcttag tatggcctgg aaccaccatc aactgctgat
     1981 gaataggctg aacgatttct gctactgctt ggatgtgagc ctgatgaaaa agatagtcca
     2041 aggtttcatg aacactaccc tcaatagaat tttcaactgg caccacagaa tagtccacta
     2101 atccttgctc ataagccttg atgacatctg taatgttggc aaaagcctgc aattcctcat
     2161 gaggaaaagc tgtctgcaca acgtggtgtg aaaatgatcc cttgggacct agataagcta
     2221 ttttcatctt agttcctcta taatttcctc tgggcttagc ttggtcacat ccaaaacccg
     2281 actagccact tcctcatacc aagcctgtct ttcttgaaaa atagctacta gttcttcctt
     2341 gctattattt agaaaaagcg gtcgctgatt gtccttatca gctgcgatac gtaggtagag
     2401 ggtttcaaaa tctgctttca ggtagatgtt atctgtatta gtcttgagta agtcacgatt
     2461 tctctgagaa ataaccactc ctcctccagt tgacacgact tggtctgttt gtagtaaatc
     2521 agctaggact tctgattcta catgacgaaa ggctgcttct cccttttcag cgaaaaaatt
     2581 cgcaatggac atacctaggc gcttctcaat cagagcatcc atatcaaggt aattagggtc
     2641 caagcctctt gcaatagtcg attttccagc ccccataaac cctaataaca ccttagccat
     2701 gaatcaagct ctccaaatca tcaaagaaac taggatagct ggtattgatg gcttctgcac
     2761 ggtcaagctc cacctctcca tctgcaacca agagggctgc gatagctgtc atcatgccga
     2821 tacggtggtc accaaacgta ttgactctag caccgtgaag agctgatttc cctttgataa
     2881 tcatcccatc tgccgtagga gtaatatctg ctcccatact atttaaggcg tctgccacaa
     2941 cctgaatacg gtctgtttcc ttgaccttga gctcctcagc atccttgata actgttacac
     3001 cttgggcttg agtcgcaagc agggcaataa tgggcaattc atcaatcaat cgtggaatca
     3061 aagcgccacc aatctctgtt cctttcaagt cagaagactc aacaatcaag gtagcagatt
     3121 tagcgactgg atcgatttca gttatttcca attttccacc catggcacga atgacatcaa
     3181 taataccggt gcgagtttcg ttgatcccca cattctgcag cactagacga gaatttggag
     3241 caatcaaacc tgcgactaac caaaaggctg cactggaaat atctcctggt acgaccacct
     3301 tctgtcctgt caatttttgt ggcccctgga ctgtgatttt cttaccatcc acacttaaat
     3361 gaccaccaaa ttgttgcaac atatcttcag tatgattacg ggtgtactct ttttcgataa
     3421 taactgactc ccccttagct tgtaaggctg caaacatcaa ggctgacttg acttgggcag
     3481 aggcaattgg caactcataa tgaataggtc ttaggttttt cgtccctttt aagcgaaggg
     3541 gaggcaagtc tcgttcagtt tgccctgaaa tgctgacgcc catttttttc agtggaaggg
     3601 tcacacggtc cataggacgt ttggaaagac tatcatctcc aaacatctct acttcgaaat
     3661 ctgcaccagc aaggacacct gaaatcaggc gaatcgaggt gccagaattt cccatattaa
     3721 gggcattttg tggcgctttt aagccagcca tgcctacacc ttgaatggta ataaccccat
     3781 ctttatcctc aatttcaaca ccaaggtcac gaaaaacctg catggtcgaa agaacgtctt
     3841 cacctcgcag aatatcataa accttggtct caccctcagc caaacttcca aagataatgg
     3901 aacggtggct gatagacttg tcacctggga cgcggatact accatgtaaa tggcgaatgt
     3961 ttgtttttag tttcatactg gacctcatac ttgcaatact tttacctatt ttatcataaa
     4021 aagtcagaaa ttccttaaaa attcctgact ttaggatcgt tcttttctta tttcagcaat
     4081 tctgaaactg gttcaaaaac aattttttca atgtcagaaa ggtaaatggc caattgttgt
     4141 tgcttggtaa agaattctga caagaggcta tttccttgaa tctgtttacc aaagccttcc
     4201 atcttagctt ggaaggacgc atctggcatt tgacctgtct gtgctagttt ttgaatttcc
     4261 tcttgaaagg caagatattc tgtaaagatt ttgcttgcct cagcatctgc tgcaatcgca
     4321 tctttagctg ctttaacagc cttgtattct ggtaatccgc gtagaccgcg actgagttcg
     4381 tttgcactat cgtaaatatt tgacatgttc ttctccttat ttgatgacga ctgtatagtc
     4441 ggtattttct gttatgagat gctcagctct ttccaagtct tgagcatttt taaatgaaat
     4501 ttgtaggatt ccgtgaatat cctcacgatt ttcctcgttg atgtggatgt taaccaagga
     4561 agttccacgt agcagttcca aaatccgcag gatgacatct tcttcatcag gaacgtcaac
     4621 atagaggtcg taagagctat ccacaccacc acgcttatgg atttccatgg tctggcgttg
     4681 ttcacgcgct tggttaaaaa agttccaaat ttgctcttca tctcccttac taatggcctg
     4741 accaatcact tccaaacgtt ccttgaaatc ctcaattcta tccagaatgg tctcgctatt
     4801 ggacaagaga atggaggtcc acattcctgg ctcgctttcc gcaattcggg tcatatctcg
     4861 aaaaccacct gccgcaaagc gccttgccat ctcatgctct tgagcataga ccgcagtctg
     4921 ctccatgaga ctagaagcca aaatatgagg aaaatggcta atctgagaag tgacacgatc
     4981 atgctccttg gcatcaatct cgataaaacg agcatgaaga cctgaaagca gatccttcat
     5041 ttccttaagc gtgtcctgac ttgtcaggct tgaaggtgta aagatataat aggcattttc
     5101 aaaaagattg acatctgccg aagcagcccc tgtcttgtga ctaccagcca tgggatgggc
     5161 cccgacaaag cgaacagact tgccagccaa atactgctcc gccgcatcca caatggttga
     5221 cttggtcgaa ccagcatctg aaataataac gccttctcgc aaatccaaat tggccaactc
     5281 cttaatgaaa gcaatagttt gtttgattgg caagctgagg ataatgacat ctgccaaagg
     5341 agcaaaacta gcaaaatcat ccgttgcacg gtcaatcata ccttctttca aggcgatatc
     5401 tctcgaagct tgactacgat tataacctaa aatttcataa tctggatgat cgcgtttgat
     5461 accaagtgcc atagaggctc caatcaaccc aagacctgcg atatagattg tttttgccat
     5521 aggaactcct taatagttct ttgtatagtc tcggtgtttg gctaccgctt cttttagttc
     5581 ctcaagatta tctgatgaga atttttcgag gatttcttgc gccagaaccg ttgctacaac
     5641 tgcttccatg accattcctg cagctggaag agcagtcgga tcacttctct ccacggttgc
     5701 cttgtaaggt tcgtgggttt cgatatccac actcataaga ggtttataaa gagtaggaat
     5761 gggtttcatg accccacgaa caacgatggg ttgcccatta gtcataccac cttcaaaacc
     5821 acctagatta ttggtacggc gagtataacc gtcttcttta gaccagagaa tttcatccat
     5881 aacttggctg cctttacgat aaccagcctc aaagccaaga ccaaattcca cccctttaaa
     5941 ggcattgata gagacaacag cttgagccaa tcttgcatcc aattttctat cccattggac
     6001 ataggaacca agaccaactg gaacgcctcc gacgactgtc tccacaaccc caccgatggt
     6061 atcaccatca cgtttgattt ggtcaatata gtccttgatt tcctgttctc gttcttggtt
     6121 gacaatagaa acttcagact gggcagctct ttgcttaatt tcagcgactg tcagattttc
     6181 aggaacatct atttccttac caccaaagac cacgacatgg ttggcaatct ccatatccag
     6241 ctcagccaag aggcgtttgg ctactgcacc aactgccacc cgcatggtgg tttcacgagc
     6301 tgatgaacgc tccaaagaat ttcgcaaatc atcaaaacgg tacttaatcc ccccaaccaa
     6361 atcggcatga cctgggcgag gatgagtaat tttccgcttg cttttaaggc ggtcttcaat
     6421 gtcctccgca gacatgatgt ccagccattt ctggtggtcc ttattgatga catccatagt
     6481 aataggcgcc cctgtcgtct tcccgtggcg aacacccgaa gtaaagacaa cctggtcact
     6541 ctcaatcttc atacgaccac cacgaccgta gccaccctga cggcgtctaa gatcctcatt
     6601 gatatcctca gctgtcaatg gaagtccagc tggaattccc tcaataatag ctgttagacg
     6661 ggggccgtgt gattctcctg cagttaaata tctcatacac tctccttatt ttaccaagta
     6721 gtctttcatc tcttccagag aaactgggtg aatggtcgct gaaccaagct ctggcaccaa
     6781 gaccaatttc aaggtgttac cacgcgcttt cttgtcatga gtaagagcct gataaagctt
     6841 gtcaactttc caattttcat agtcaacagg caagccgaat ttctgacaca tctctgtgat
     6901 agattgggta atgccagctg gcatgaggcc tttttcctca gcaaccttgg aaatctgtac
     6961 cattcccatg gcaacagcct ctccatgcat gaccttgcca taaccggcag tcgcttcgat
     7021 ggcatggcca atagtgtggc caaaattgag gtaaagacga ataccattgt ccaactcatc
     7081 ttcaaccacc atcttgcgct tcacctgaca agaatgttca atcaaggtct ctgcatgttc
     7141 caaaatactc tcaacagaac cattcagtcc cgtcaagaga gcccacagtt ctggatcctc
     7201 aatcaagcca tacttgataa cttcacccat cccttcaatc aactctcttt ttccgagggt
     7261 ttcaagaaca agtggatcaa tcagaacccc atctggttgg gcaaaggtcc ccaccatatt
     7321 tttagcaaat ggtgtgttaa cgcctgtctt tccaccgata gaagaatcaa cctgagctgt
     7381 caaactagtc ggaatctgaa caaagtgaat accccgcata taggtagagg ctacaaagcc
     7441 agccaggtcc ccaacaacgc caccaccaag agcaacgatt ccatcgctac gagtcagacc
     7501 ttgcttgact agaaattcat agactttctg aacagtagtt aaattctttc tttcttcacc
     7561 ttctaagaaa tcaaaaacag ctacctgaaa accagcatct tctaggctga gcttgacctt
     7621 ctctgcatag agagaggcta catggttatc tgtcacaatg actacctttt gcggttgcca
     7681 gagttctcgc aaccactgac cagcctgggc catacaacct ttttcaatct gaatatcata
     7741 aggatgatga ggaatatcga ttctgatttt cataggagag tctccctttc tttattgata
     7801 tttttctgtt aaagactgcc aaatctcttc tgtcggcatt ttcttgcctg tccacagttg
     7861 aaaagcttct gcagcttgat agagcaacat tcccagacca ttgactgctg gattgccctg
     7921 acttctagcc catttcaaaa acggtgtttc aaagggttgg tatatgatat ctgcaactaa
     7981 gagagtttct ggtaagacta tgttttcagg aacaggagag gattggccat ccatgcccac
     8041 actggtggca ttaactagca aatccgactc ggcaatcctt gcttgcagtt cagaaacata
     8101 ttctaaagca cacaaatcca ctttaaagcc tgtctgctcc tgtaacttgt ctaggtaagg
     8161 tcttgttttt tccatagaaa cggaacgaac aaagaccgaa atctgactga cgccatccaa
     8221 aatagcctgt gccaagattg atttagccgc accacctgca cccagcaggg tcatcttttt
     8281 acctgaaatt gtaaaagaag gcaagcactt aaaaaatccc ttgccatctg tattatatcc
     8341 aattaaattg ccattctcat tgacaaccgt attaaccgca ccaatcaagc gcgcttcatc
     8401 gctcagctca tccaaataag gaatcacctg ctccttatag ggcatggaca gattgatgcc
     8461 aaacatctgg tagcgacgaa tattggccac tgtttctacc aagtcactcg cttcaatctc
     8521 ccaagccaca taagcaccgt tggtagctgt cgcctcaaag gctctattgt ggatgaaggg
     8581 agaaatagaa tgcttaatag gattggcaac aactgcagct aaacgtgtat agccatcaag
     8641 cttcatccaa aatctccctg atttttttca tgctagctag agaaatctgc ccaggggcac
     8701 taacctcatc cagactggca aaagaccaac tcgaaccagt cacatccgca gtgatacgag
     8761 agaccttgcc caccttaccc atagaaatgg tcacatattc ctgttcagga ttgagcgttt
     8821 taaagcctcg tgtatagttc atcaagtcta agacatcctg ctccgtgtga gccatcaccg
     8881 caaccttaac aagttttgga tttaggatcg tcaactctga caagatttcc atcatgttct
     8941 caggtgtttc ttggaaatta tggtaactca aaacaagatt tgggaagtcc agcatttcct
     9001 caaaaacatc cttgtagcta tagtactcaa aatcaatata gtctggttga tagagttgcg
     9061 caacttcctt gattagatgg atatactctt ctggagaaag gtcgatttct ccaccttcgg
     9121 agcgagttcg tagcgtgaaa accaactcac ggcctgcgaa tttttcaaaa atggctggag
     9181 ctacctgcaa aatcgcttct ttaggcagat agtcggcacg ccattcaatg atgtcggcat
     9241 ccaggtacct cgtggcatcc agagcctgag cctcctctaa acttcttggc attactgaaa
     9301 cgattaattt catttactaa ccttcatact aatcaccttg aggtaattac tactttcatc
     9361 ttttttatta taggcaaaat ctgctggaag accatatttg tttaaaatct ggtaacttct
     9421 tcctgcaaag cctttatcaa tttgttctgt aaatttctga cgggaaacat tggcagcatt
     9481 ggtactggca atgataatcc ctcccggatt taaaatctca agactctggg aaatcaactt
     9541 gtgataatcc ttggccacag agaaagtttg ttttttattc cgagcaaagc taggcggatc
     9601 taggacaatc acatcgtagg tcaagttttt gcgtttggca tatttgaaat actcaaagac
     9661 atccatgact ataaaacgac gctcgtctgt gctgagccca tttgcctgaa aatgcgcttg
     9721 agacaattct cgtgaacgtt tggctagatc aacagaagtt gtctggctag ctcctcccat
     9781 ggccgcagct actgaaaaag ccgctgtgta ggaaaacata ttgagcaagg atttacccat
     9841 agccaagccg tcaactaaac taccgcgaac ctcatgctgg tctaggaaaa ttcctgtcat
     9901 caagccatca ttcataaaga cttgatacag gacaccattt tctaaaacag tgaaaaagtc
     9961 aggtgcttct tgaccataaa catgggcaga ttcatagtcc aaacccttaa agcggatttt
    10021 ctcataagcc cctaaaacct cagggaaaac ctgtctaaag gcttctgata tagtctgacg
    10081 aatctgataa acataagagt tataccaaga aaagacggcg tagtcgccat aaaggtccac
    10141 tgtcagaccc ccaaagccat ctccctcttg attaaagaga cgaaaggcag ttgtcaaatc
    10201 atcttgatag taggcgtttc tcttttcttt ggcttttcta aacaacgttt caaagaaagc
    10261 ttgattgaag gccaccttgt ctttgctgac aaaccagccc aagcccttgt tttgctgaga
    10321 aaggtaggca gtcccaagaa agtttccttc ctgaccctgc acctctactt cctgatcctt
    10381 aagattgaca ttctcaagat cactggcttc tagtaaaact agccccttag caagcttctt
    10441 ttcaaccctt ttgctgactc ttattctatt cataactacc attatatcaa acttttagac
    10501 aattctcaaa aaagaaacta cccttgcttt tttactcttc ttttaaaaaa tggtatacta
    10561 gacttcctgc aaaactagga agtaaatgtg taagaatcac aataaaaaat gctcttccgt
    10621 cttggaggag catttctttt tatcaacgaa aatcaaataa caaactatga aactagcctc
    10681 aggttaactg tgagattata ggtagagagg ttgaatcagc aatatgtgtc tgtcaaattt
    10741 agtgacaaag gtagtagaag aaagatgaag aaataaatct gcttcagtag atatctggaa
    10801 aatttgattt tatagaaaag ccttttgtta caaactcaat atactatcaa taaataatat
    10861 tatagaagca acaataatta aaatttcacc tatttgcatc attctatttc gaactctaaa
    10921 tatatgttct atcaaaaata cttggatcac acacattata ggaattaacg tttttgaaat
    10981 tgaaaaatat cccaataaat aaactataaa caacaataat agaactatat tatatttctt
    11041 attcaaaaca ttcctcccta tatttttgat taccaatctt aatcatttac aactacattc
    11101 taacaaacta taaaagcgtt tgtcgaattg aatttatcaa gcaagcaacc aactatctca
    11161 tcttttttct atttctgcca atatgcgtga caggtaataa tgatagccaa aaatagcaag
    11221 agcaagcaag acgataagag ctcctactcc caagttgatg gcaaggatag gggagagaga
    11281 ctgaaccaag aatatgctcc caattacaag ggccatcagg attgcactat aaataaacaa
    11341 taaaactatg gcgactatgc catttgaacg attcaccagg tccgtaatgc tactccaatt
    11401 ggttgacaga tttttaacgt ccttaaagta atggtggcaa gaaaggatga cactggcaat
    11461 gatccagact acaagaaggt aaatcatcga aaggatggac aagcctagat atagagaaag
    11521 accgagcaaa gtcagaactg gtaaaaagga ctgaacagca aagataatcc aaaatttcac
    11581 tttcacataa cgagcaaagt caaagggtaa actcttcaga aaatcaacat tttccctctc
    11641 caaggacaag gcaattgaat gcaggctggt gatattgtta ttgacaactg ctataaacac
    11701 tcctacaaaa aacaagggta accagtatgg aggatgaatg tctggaacta tctgagaatc
    11761 tcggattttg gaaatcagac tgatcatcat gagataagga aggaaagcac ttgtaaaaag
    11821 cactgtaatc acaccagtcc cctatcccaa gagggtgagg tggtagcgta aaaccatacg
    11881 gaaaaagccc tttttagtag ttgaaatcct ctccttgctg cgacgttctt ttttgacctt
    11941 ctcctcactg ttaagcagga tcacgtcata aaaacgagga aggaccttct ttttggtcag
    12001 ataaagcagg aagagagtta gtcctatcca agcgagcaga cccactaagg cttctgtcga
    12061 aaaaggctcc actgctattt tgtaaaagat atgaagagga taaaggagaa atggaatgtc
    12121 tctaactttg tcaacaatac ttccaaaagt cgactgaaga aagaagataa atattaaagg
    12181 tatgagaact cctatcccaa tcatcacatt cgaaaaaata gactgatact ttctgaagac
    12241 cctagtctga gccaagaaat gtactgccac taccgtcact aaagtaacag agacaaataa
    12301 taaggtcaag gacagtagca tcaaaggcaa acccagccaa agagaaggag ctagactaat
    12361 atagagggct agaaaataag ctaggattgg tacaattcca gttagagctg gcaagaggac
    12421 agacagacct ttggccatta taatctctga ttctttaaag gcatagggcc tataggaggc
    12481 caggtcctta ctctcataaa agacattgta aaaggcagtg aaagaagttg aaaaggccag
    12541 caccagtaaa atagcaacca tcgagctaaa ataaataggt atttcctcaa aaggaaaatg
    12601 aatggctata ttactaaaac agatgagcat caagagactg gaaaaaatgt aagaacttag
    12661 gactctagca gaaacattta cttttttccc aggattctta gcctgcttct ttcgtagatt
    12721 agccagatta gcttcttgag atgaatagag gatattgata tcaactaatt ttttaatgac
    12781 cttgagacgc atccgcaacc tcctcttttc taccagcaag actaaggtag atactttcca
    12841 aaaactggtc tgggtggtct ttcctcaagt tctctacact accacaatag atcaaatgcc
    12901 cctttttcaa aatagcaatc cgatcacaga cttgctcggc tacctcaagg acatgagttg
    12961 aaaacaagac cgtcttgcct ttttgtgcat gttccttcat catttgcttc aaatcaaagg
    13021 cggcctgggg atccaaacca gtcaagggtt cgtccaaaac ccaaatatca ggagcagaca
    13081 agagtgcccc aatgacaaag actttctgac gcattccgtg agaaagagtt tcaataacct
    13141 gatagcggtt ttcagcaaaa tcaaaaacgt tcaatagcct agctagacta gtctccaagt
    13201 cagagctagt cagatcatag gatgaagcga tcaattccca aaattcattg gccgttaagc
    13261 gtaaaaataa gtcaggcgag tctgctacgt agccaatctt tcgtttgatc gccaagcgat
    13321 tttccgataa ctcctgaccg tctaccaaaa tacgaccact gctgggtgaa atgatactga
    13381 ctagggattt tatggtggtc gattttccag ccccattatg accaatcaag cccataatct
    13441 ctccattttc aatctgcaaa ttgagattac tcaaggcctc tttatcacca tacaacttac
    13501 taacattttg aaattcaatc atgggacaac tcctatcttt atctgtatta tacatcgcta
    13561 tactagcact ttgatacaaa aaaatcaata ctttatttta agtagttaaa atagtttcct
    13621 tctatcttac gcaaacgttt gcgccgagag atactttatg atagaataaa gaacaagatt
    13681 gacaagtaag aggaaacatc atgcaaaatc aaacactcat gcaatacttt gaatggtatc
    13741 tgccccacga cggtcaacac tggacgcgtc tggctgaaaa tgctccacac ctagctcatc
    13801 tggggatcag tcacgtctgg atgccaccag ccttcaaggc aaccaacgaa aaagatgtcg
    13861 gctatggggt ctatgactta tttgacttag gagagttcaa ccaaaaaggg actgtccgca
    13921 ccaagtatgg tttcaaagaa gactatcttc aagccattca agcccttaaa gaacagggaa
    13981 ttcaacctat ggccgatgta gttctcaacc acaaggctgc tgccgataac agggaagcct
    14041 ttcaggttat cgaagttgat cctgtagacc gtacagttga acttggagaa cccttcacca
    14101 tcaatggctg gactagtttt accttcgatg gtcgccaaga tacctataat ggcttccact
    14161 ggcattggta ccacttcacc ggtacagact acgatgccaa acgcagtaaa tctgggattt
    14221 atctgatcca aggggacaac aagggctggg ctaacgagga attggtcgat aacgaaaacg
    14281 gaaactacga ctacctcatg tatgccgacc tagactttaa acatcctgaa gtcatccaaa
    14341 acatctatga ctgggctgat tggttcatgg aaacgactgg tgtagctggt ttccgtttgg
    14401 atgccgttaa gcatattgac tctttcttta tgcgcaactt catccgcgat atgaaggaaa
    14461 aatacggtga cgatttctat gtttttggtg aattttggaa cccagacaag gaagccaatc
    14521 tggactatct cgaaaaaacg gaagaacact ttgaccttgt cgatgttcgt ctccaccaga
    14581 atctctttga agccagtcaa gctggcgcaa actatgacct tcgtggcatt ttcacagata
    14641 gcctggttga actcaagcct gacaaggctg tgacttttgt cgacaaccac gatacccaac
    14701 gaggacaagc ccttgagtct accgttgaag aatggttcaa gccagcagcc tatgccctca
    14761 ttttgttacg ccaagacggc cttccatgtg tcttttacgg agactactat gggatttcag
    14821 gccagtatgc tcaagaagat ttcaaagaaa tccttgaccg cctcctagcc atccgaaaag
    14881 acttggccta tggagaacaa aatgactact ttgaccatgc taactgtatc ggttgggtac
    14941 gttcaggtgc tgaaaatcaa tccccaatcg cagtccttat ctcaaatgac caagaaaaca
    15001 gcaagtcaat gtttgtcggt caagaatgga ctaatcaaac ctttgtagat ttacttggta
    15061 accaccaagg tcaagttaca attgatgagg aaggttatgg acaattccct gtctcagcta
    15121 gatccgtaag tgtctgggca gtcaatacca tctaatagct cataataacc aagctaggtc
    15181 caagcggatt tggctttttt gtattcacaa aaagacctac ccaaatggat agatctttac
    15241 ttgattacaa tttacctgct actgcatcca acaattcttg gatcttagct tggttgcttc
    15301 ctcctgccat ggccatgtct ggtttaccac caccacgtcc atcgatgatt ggtgctaatt
    15361 ctttgacaag gtttcctgca tgaaggtctt ttgtcttgct tgctacaagg acattgactt
    15421 tgtcaccgat agcggcaact aggacaagaa gatcagagta gtctttttgt ttccagttat
    15481 ctgcaaaagt acgaagggca ccggcatcgg atacagacac ttgactagca atgtaacgat
    15541 gaccgttgac ttccttagca tctttgaaga tatcgcctgc ggctgcagct gcggcttttt
    15601 ctttcaactc agcattttct ttttgaagtt gacgaagttg ttcttgaagt ccttctacct
    15661 tgtgaggtac ttccttgact tgaggtgctt tcaaggttgc tgcgacagct ttaagagcat
    15721 cctcttgttc acgataggct tcaaaggctt ccttaccagt cactgccaag atacggcgag
    15781 ttcctgaacc gattccttct tctttgacaa ttttgaagag accaatctca gaagtgttgc
    15841 caacatgagt accaccacaa agttcaatag agtagtcacc gatagtcacg acacgaactt
    15901 ccttgccgta tttctcacca aagagggcca tagctcccat ttctttagca gtgtcaatat
    15961 ccgtttcaac tgtcttcact tcaagtgctt cccaaatttt ctcgttaact tgctgttcaa
    16021 tcgcacgaag ttcctcagca gttactgctt ggaagtgggt aaagtcaaag cgaaggaatt
    16081 caacttcgtt aagagatcct gcctgtgttg cgtggtttcc aaggatattg tgaagggcag
    16141 cgtgaagcaa atgagtcgca gtgtggtttt tcatgacacg gtgacggcga ttgctatcaa
    16201 ttgccaaggt atattcttgg ttcaaggcaa gcggtgcaag gacttcaact gtatgaaggg
    16261 cttgaccatt tggggctttc tgaacattgg tcacagtagc cacaatctta cctgactcat
    16321 ccaagatttg tccgtagtca gctacctgtc cacccatttc agcataaaat gacgtttccg
    16381 caaagataag agaggcagtt ccttctgaaa cagctcctac ttctgcattg tcagcaacga
    16441 tagctaccaa tttagaagac aattggctag tattgtagtt gaagacactt tctacagtga
    16501 tgttttgaag agtttcattt tgcataccca ttgagccacc cttgacagct gacgcacgcg
    16561 cgcgttcttg ttgttctttc atggctgctt caaaaccttc acggtctaca gtcataccag
    16621 cctcttcagc aatttcttca gtcaattcaa ctgggaaccc ataagtatca tagagtttga
    16681 agacatctga accagcgata acagattgac ctttttcttt caagtctgct acaatgcctt
    16741 gggcaaagtg ttgacctgag tgaagggtac gggcaaatga ttcttcttcg ctcttaacga
    16801 ttttctcaat aaagtcacgt ttctcaagca cttctgggta gtagctttcc atgatttttc
    16861 caacagttgg aaccaatttg taaaggaaag gctcgttgat tcccaatttt tgaccatgca
    16921 tagaagcacg acggagaaga cgacgaagaa cataaccacg accttcattt cctggaaggg
    16981 caccatcacc gatggcaaat gaaagtgaac ggatgtggtc agcgatgacc ttgaagctca
    17041 tgttgtcgcc atcttggtca taaaccttac cagacaattt ctcgacttca cggataatcg
    17101 gcatgaagag gtccgtttca aagttggtct tagccccttg gataacggcc accaaacgct
    17161 ccaaaccagc gcccgtatca atgttcttat gtggcaattc cttgtattcg ctacgaggaa
    17221 cagcagggtc tgcgttaaat tgtgacaaaa cgatgttcca gatttcaata taacggtcgt
    17281 tttcaatatc ttctgcaagc aggcgaagac cgatattttc tgggtcaaag gcttccccac
    17341 ggtcaaagaa gatttctgta tctggtccag aaggtcccgc accgatttcc cagaagttgt
    17401 cctcaattgg aatcaagtga cttggatcca ctcccacttc aatccagcgg ttgtaagaat
    17461 ctttatcgtc tggatagtag gtcatgtaaa gtttttcagc agggaaatca aaccattcag
    17521 ggcttatcaa aagctcataa gcccaagtga tagcttcgtc acggaagtaa tccccgatag
    17581 agaagttccc caacatttca aacatggtat ggtgacgcgc agtcttccct acgttttcga
    17641 tgtcgttggt acggatagcc ttttgggcat tggtaatacg tggattttca gggataatgg
    17701 tcccgtcaaa gtatttctta agggttgcta ccccagagtt gatccacaaa agagttgggt
    17761 catttacagg aaccaaactt actgatggtt ctactgagtg acctttggtc gcccagaaat
    17821 caagccacat ttggcgtact tgtgcactag atagttgttt catattgtct ccttatttac
    17881 ttgtttaatg tgattggctt tccagcattt ccacatagtc aatcgcgaca cagagggaaa
    17941 tgactaggtc tgcataagcg tcttcaagaa ccgttacggt ataggtagag gtcagatgga
    18001 agagttcctt cttaatttcc gcaatcaact gatcgcgatc atccagcaat ttgaaattca
    18061 aatcccagat attgccctcg atacgaagac ctagattatc aaactcatac ttatctcgcc
    18121 aaaaggtcaa cttcttacga atgacaaaac tcgagccatc cagaagctga atctcaaaac
    18181 gaggaagcaa ggtcaagatt tctttactga tctgactgac ttgttcacca gccgcatcat
    18241 agatggtaaa agttttggga atcttaaaaa atgatccctc cacctgatag gcaatttctc
    18301 ccctgtcatc cttgatagcg aagcgttcgc ctccaagacg aaacttttgt ttgacaagaa
    18361 atgttttcat caacacctcc aaaaatcaaa agacaagctc atatcacgaa gggcgaaaaa
    18421 ccgcggtacc accttcattc aatgaacttg tcattctctt gttcttatgc aattgtatga
    18481 ttgagtagca tgacttccta gcttagatgg ctcgcagcac cgccatttct ctggactaag
    18541 acaagtgaaa atcaattctc aactttctta ttatatcgtt tttttaatct tgcgtcaact
    18601 ggaaatgatc tccgttgaat tagaccaatt ccctacatct ctgattactt ttccaggata
    18661 tattttttct tactgccgtt tttcttttta tcccaaattt tcatattact aaacacagct
    18721 actagaatat ttccaaatat aaaggtgcct atcacccaat atatggactc agttgttagg
    18781 tattgtcgat ccaagccatc ctttaaatgg aatagtatag cagtttggtt aacaatcata
    18841 aaggttggcc agaaactttt gttgaaaaaa gtagacattt tcattatttg ttgccgcttt
    18901 ctgtaaggtt aatactcaat aaaaatcaaa aagcaaacta ggaagctagc ctcaagctgt
    18961 acttgagtac ggcaaggcaa cgctgacgtg gtttgaagag tataggctta gtatactact
    19021 aggcaagcaa ataagcaaat aaacaactag aatagaaaaa gatagggctc taaaaactga
    19081 cttctattcc ttaaaaacga accagcttga ctgattcgtc ttcttacgtt tatctcctac
    19141 ttccgataca ttttaaactg taggaagagg tcgctatatt tccctgtcca tttatggtca
    19201 aatttctcat aaatttctag gtgtttcatg gtttcaacat cgggatagaa ggccttatct
    19261 tcctttgttt cctctgggag caattccttc gctggtatgt ttggtgttga atagccgaca
    19321 tactccgcat tttggagagc attttcaggt ttcaacataa agttgataaa ggcataggct
    19381 gagtcttggt ttttaactgt tttgggaatg accatattgt caaaccaaag attgctggcc
    19441 tctgtcggta ccacataacg tagattttca tttttttcta acatttggct ggcttcacca
    19501 gagaaggtca cgccgattgc aacgttattc tgaatcatat agcccttcat ctcgtccgca
    19561 acgatagcct tgatatttgg agtcagtttg tagagcttat ccactgtctc ttccaactgc
    19621 tgcagatcct tggagttgag gctgtagccg agggaattga gtcctagtcc cagcacctca
    19681 cgcgccccat caaagagcat gatagaattc ttatactccg gcttccaaag gtcatcccaa
    19741 tgctcaggcg cttcatctac catggtttcg ttgtagacaa ttcctaaggt tccccagaag
    19801 taagggatgg agaatttatt acctgggtca aaggactggt tgagaaactc tggtccgata
    19861 ttttcgattc cttcaatttt tgaataatca agcggaacca agaggtcttc gtccttcatc
    19921 ttgttaatca tgtattcact tggaatggca atatcgtagg tcgttccacc ctgctttatc
    19981 ttagtgtaca tggcttcgtt ggagtcaaaa gtctcgtact gaacttgaat tcctgtttct
    20041 tctgtaaact gagtcaagag ttcaggatcg atatagtctc cccagttata gataaccaat
    20101 ttttgactat ctcgactatt gattttacta tctaaatgag tcgcaattcc ccacaagaca
    20161 aggataatcg ctgcaattcc tgctaaaaat gaatagattt ttttcatgct tgctcctcct
    20221 tctcacgaga gataaagtaa taacctacaa ctaggataat actaaagaga aagactagag
    20281 cagacagggc attgatttct aaggaaatcc ccttgcgagc acgagagtaa atctcgactg
    20341 atagggttga aaagccattt cctgttacaa agaaggtcac ggcaaagtca tctaacgaat
    20401 aggtgaaggc catgaaataa ccagtaatga tagacggagt caggtaagga agcatgattt
    20461 ccttgaacat ctgaaattga ctagctccca agtcataggc cgcatgaatc atgtcgccat
    20521 tcatttcctt gagtcgaggc aagaccatca agaccacgat aggaatggag aaggccacgt
    20581 gactagatag aacggtcaaa aagccaagtg aaaacttgag ttgggtaaag agaatcaaga
    20641 agctagcacc aatcataacg tcaggcgcaa ccatgaggat attattgagt gatagaaagg
    20701 cttcttggta tttcttacga gactggtaga tgtaaatggc accaaaagtc ccgataatgg
    20761 tcgctatcaa ggctgatagg aaggccaaga aaaatgtctg agccaaaatc agcatgagtc
    20821 tcccatctcc aaacatggtt tcaaagtgag tccagctaaa acctgtaaag ctattcatat
    20881 catcaccagc attaaaggca tagccaatca agtaaaagat aggcaggtag aggaccagaa
    20941 agaccagtcc cagataaagg ttggcaaatt ttttcatcgt tctctccttt ccttagtcac
    21001 ccacatggtg atgaacatgg tcaggatgag aatcacaccg atggttgaac ccataccata
    21061 gttgtcattg gttagaaaat tctgctcaat agccgtcccc aaggtgataa cgcggttccc
    21121 accaatcaaa cgggtcagca tgaagagact caaacttggg ataaagaccg actgaacccc
    21181 acttcgcaca ccgttcatag atagagggaa gatgacatga cggaaggtct cccacttagt
    21241 tgcaccaagg tcataactag cattgatgag attattatcc atgtcgtcta agacattgaa
    21301 aatcggcaaa atcataaagg ggagctcgat gtagcttgcg acaaagataa aggaaaaatc
    21361 ggtaaaaagc aactgttgtg aaccaattcc gataaattcc aagaattggt taatagagcc
    21421 attttgacca aaaatcccga taaaagcata ggctttaagg agcaaattga tccaggtagg
    21481 aaggataatc agcatgagcc agagttgacg gtgcttgaga cgggtcaaaa agagggctgt
    21541 tggatagctg ataagcaagg tcacaaaggt cacaatccct gcataaagca ctgagttgaa
    21601 actcattttg aggtaggtca agttttgtga cgcaaagtaa gatttataat tttctaaact
    21661 aaactggcct tcaatgttga aaaaggattg tccgaaaatc aagaccaagg gtgccaatac
    21721 aaagagggca atccaaagca tgtagggcac tacaaagagt ttagagcttg ttttcttcat
    21781 ctctttcctc ctcgatcgca ttgatcagac ctgcttcttg ctcttcgatt tccacgtatt
    21841 cttcgatacg agcatcgaac tcttcttcgg tttcattgag acgcatgatg tggatgtctt
    21901 ctgattcaaa gtccagaccg atttcctcac ccacgatggc cttacgggtt gagtggatca
    21961 tccattcatt tccaagttcg tcataggcga taatttcata atgaactcca cggaagagct
    22021 gggtgtcgac cttaacttgg agtttgcctt cttcaggaag ggtaatgcgc aagtcctctg
    22081 gacgaataac gacctcaaca ggttcatttg gcttcatccc accatcaacc gcttcaaagc
    22141 gtttgccgtt aaattcgacc aagtagtcct caatcatggt acctggcaag atgtttgact
    22201 ccccgataaa ggtggcaaca aagtggttga ttggctcatc gtagatgtcc acaggggttc
    22261 cagactggac aatctcgcca tcattcataa cgaaaatcca gtcactcatg gcaagagctt
    22321 cttcctgatc gtgagtgaca aagacaaagg taatgcccaa tcgttgttgt aattcacgca
    22381 attcgtactg catgtctgtt ctcaatttca agtccagcgc tgataaaggc tcgtccaaca
    22441 agaccacacg gggttggttg atgatagcac gggcgatggc cacacgctga cgttgtcctc
    22501 cagaaagttt gcggatggaa cgtttttcat aaccttccaa ctgaaccatc ttgagaactt
    22561 ccgctacacg ctgctcgatt tctttcttat caattttacg caagcgaagt ggaaaggcaa
    22621 cattttcaaa cacattcata tgtgggaaca aggcatagga ttggaagacg gtatgtacgt
    22681 cgcgcttgtt ggttggaata tcattgatac gaacaccgtc tagcatgata tctcctgtcg
    22741 tcgcatccag taaacctgca ataatgttta ggatagttga tttccccgaa ccagatgcac
    22801 ctagaagggt gtagaatttc ccttcttcca actcaaagtt gatgtctttg agaaccttgg
    22861 tgttgctgtc ttcaaaaact ttagagacgt ttttgaattc gataattggt tttttcaatt
    22921 ggcataaatt ccttcttttt catagattaa ccgatcgggg ctctgtcagg tccccactac
    22981 ctcttgcagg gagtaaaacc acctgcatac atcttcgcta ccgataggct ttcacccaag
    23041 atccggactt ctctttcaag cgtaatacct gagtgttcct tgactttttc gataaccgat
    23101 tggatcaagt cctcgtagtc tttggccgtt ccatctgcga cattgatcat aaatcctgca
    23161 tgcttttctg acacttctac gccaccgata cgatagcctt tcaagccagc ttctgaaatt
    23221 aactgacctg caaaatgccc gactggacgc ttaaagaccg agccacaaga tgggtattcc
    23281 aaaggttgct tgagttcacg taggtgcgtc aagcggtcca tttcctgctt gataacctga
    23341 tgggttcctg gagctagggc aaatttaact gacaagacaa ctgcaccaga ctcctgaata
    23401 gctgaatggc ggtaaccaaa agccaagtct ttagcagaca gggtttcgat ttctccatcc
    23461 ttggtcaaga ccttacaaga ctgcaagatg tgagcaatct cgccaccata ggcacccgca
    23521 ttcataaaga cagcaccgcc aacgcttcct ggaataccac aagcaaactc aaagccagtt
    23581 aaactatgac ggagggcaat gcgagttgtt tcaatcaagt tagccccagc ttctgcttca
    23641 atggtatagc catcaacaga aacgttattg agcttgtcac acaagatgac aaatccacga
    23701 atcccaccat cacgaacgat gatattgctt gcattgccaa gaaccatcca agggatattt
    23761 tcttggttgg caaatttcac aaggcgagcc aactcaaaac gatttcgtgg aaagaccaaa
    23821 taatcagcct ctccacctac ttttgtataa ctatagctat gcaagggttc ctgaaaacgg
    23881 atatcaattc cttctaagat ttcaagcatt ttttctctta cagacatgtc actcttcctt
    23941 ttccaaaatt cattccatta taccattttt agagacattt gacgaccata aaaatacctt
    24001 gtttggattt tgcataagaa aaagaggttc cccccttttt atgatttttt acaaaagatt
    24061 tccttggttc cataggcgac cagaacgagc tccagtgcta gaatcacttc aaccaagact
    24121 ggatttgtca accagcctac ttggaaaaga gatggtgcca gatcaaagaa ggcatgcaag
    24181 ccataggctg ttaggagata aatccatttc ttctggcgaa cagcttggta aacccaaact
    24241 gtcaaaagta attggaaacc aagcgccaag attcgctcaa aaccaagcaa ataaatctgc
    24301 cagaccgaaa gtgactgaat ggtttttaac atattttcag acagtaattg cataacctgt
    24361 ggattctgag tttgaactgc cgaaagaaca atgtaaagat tgagtaaact agtaaggcct
    24421 agaaaaatca actccaagcc accatgcccc agtccatagg ccaaggcgtc tgccttttcc
    24481 aaacttctct ttttctcaaa ccatttgaag aaaacaagac gagccgtttc ctcaaaaaat
    24541 gctgccatgg ctaggccata gataatatag ataagcggat gatcttgcaa gagggcaata
    24601 ctaccgtctt tttgagggtg caaaatcaag atatgcacca gtttttccaa aatctgcgaa
    24661 gagacaaaga aagcgactgc acccaagccc aagacagcta gattaatctg gtatttcttt
    24721 ttggcaaacc aaatgcttcc tatcagaaaa gctagcaaca gcaacatggt aataatgata
    24781 tgaatggtca ttttcttctc ctattctgcc ttttcaatat cttttttcat ctcgtcaaca
    24841 ttgaacttag caaacaagta ttgacggtct tggactggga aacgttggtc caactggtca
    24901 actgctccca cctcgataat gccttccttg atgacaaaat ccatgacatg tccaccgaag
    24961 gtcaaatcat ctgatatgaa gtgcagatgg tagcctgcca cactgacccc atggaaaatc
    25021 tcaggcgtcc agaaaccaac aatagtcccc gcaacattgt cacgactata ttccggttga
    25081 tgggttgcga catcagcaaa cttggtatcg ggtgttgact taggaatcat acgcacatgc
    25141 atatgtaaaa attccccccg aatcttgata gagcggaaaa gattttcccc atcataataa
    25201 gactcaattc gttctttcaa ttccttgtct gtcatctcaa agcgctggcg gaaaatgacc
    25261 tctgcctgat gcggtaccac tgcagcgtaa ggaataaggg catctggtga cacttccaca
    25321 atttctggct ggtctcctga ccctttggcc tgataagcct tgccatccaa gacaatcaat
    25381 tccccatcaa tggaatccaa ggttcccaaa ccaaggtcac catgttctag caattctcct
    25441 actgtcatag taccaccata aaggccagcc atcaaggctc caagagtatt gtattgaaat
    25501 aatttcactg gttcctgcac gttcttatcc attcactttc ttgtctgtta ttctataaat
    25561 cttactccta agtataccac atttgcccct agatgtgaac gagagaaacg ttctagacat
    25621 tgccaagaag gaaaaaaaag ggtacaatgt aacaaaatca agggaggtct ggaatgaaga
    25681 aacaaagcaa gtacaaagag gtcgtttcct atctgaaaaa tggtatcgag tctggacgat
    25741 ttccgacggg tagtcgcctg ccttctatcc gtcaactgag ccttgacttt cactgcagca
    25801 aggacaccat tcaacgagcc ctgctggaat tacggcacga acaatacctc tatgccaagc
    25861 ctcagagtgg ctactatgta ttagaacaag ggcaacatca agacctagaa atcgaggtta
    25921 ccgacgaaca tgccagtgcc tatgacgatt tccgactctg tgtcaatgaa accttgattg
    25981 gccgagaaaa ctacctcttc aactactatg acaatcaaga aggattagaa gacctaagac
    26041 agtccattca caaactcctc tttgagcaag ctctctactg caaggctaac caactagtac
    26101 tgacttctgg aacccaacaa gccttattta tcctctctca aatatccttt cctagacaag
    26161 ccaaggaaat cttggtggaa cagccaacct accatcggat gaatcgcctc ttgattgcac
    26221 aggggctgga ctatcaaacg attgaacgag gcattgatgg gattgacttg gaggagctgg
    26281 aaggccactt caaaacagga aaaattaaat ttttctacac cattccccga tttcactatc
    26341 ctctgggaca ttcctattct gagcaagaca aacgatctat tcttaactta gctgccaagt
    26401 atgatgtcta tatcgtagag gacgattatc tgggtgattt ggactccaag aagggccaaa
    26461 ccttccacta tcttgataca gaggagcatg tcatttatat caagtccttc tcgaccagcc
    26521 tttttcctgc ccttcgtatt acagcactca ttcttccaaa tgctatcaaa gaagcatttg
    26581 tggcctacaa aaatatccta gactacgaca gcaacctcat tatgcaaaag gccctgtcac
    26641 tctatattga cagtcaattg tttgaaaaaa atcgtttggc tcgcttgacc aatcatgaat
    26701 cttaccaaaa acaaatcgag gaaaggataa ctaaaacacc ttgtcccctt cctcattatc
    26761 ccctacacga tggtttattg ctagacctga gacagtatcc taaaatcgcc agtctcaaac
    26821 acagtcaact gggcttggac ttctttgaag aggcctattt gagcacctgt ccttatcaat
    26881 ttgccaaggt gtccctagac aatctggaaa atgttttaaa ctatttaaaa gcagaattgg
    26941 aatgacatcc aactctgctt tttcttattc ttcaacttca gttagacttg ctgctttttc
    27001 aagatagctt tggtaagttc cttcatcctt aagtttttgg atgaccttgt ctaccacttc
    27061 tttcaaatca tcactatttt ttctaagagc aacggcattg gcgtcgccgt ccttcatctt
    27121 caagctgaca gttgcgacag ctaagccagc gtttttagca gcataactaa gtgcaacagg
    27181 ctcatccata tgaacagcat ctatttttcc agcctgcaat tcattgactg cttcacccat
    27241 attagttagg gaagttaatt gagcttttgg caattgttcc ttgaccattg attctggaac
    27301 agtccctttt tgggctgcaa tattagcact ttctaggcta gttaaatcct tgtatttttc
    27361 cacatcagcc ttacgaacca agaaactaat cttgttttca tagtatggga ttgaaaaatc
    27421 aaagacttct tttctctcgt cagtagcact aattcctgca actgctaggt cagccttacc
    27481 agtttgaaga ctggtcaaaa cattgtcaaa actcatgctt aagatttcca acttaacccc
    27541 aagttcatca gcgatagcct gagccatgtc gatgtctgca ccgactacct ggttctttcc
    27601 atcaaccaat gattgaaatt caaagggtgc atagtcagga ctggttgcca caactaattt
    27661 ccccttttgc ttaatggcct caacagctga ctgagaacca ttagaacctg actggcaagc
    27721 tactaagaag aaacttgcaa gaaaattaca taaaacaaat atccattttt ttgatttcat
    27781 aaataaacaa cctttctgtt aattttgtat aattataacg atatccaagt tacttgtcaa
    27841 gtgtttttta aatttttatc tcaaaaatat tttttcgttc aaaaaaagga gccatcagtt
    27901 gatttcaagc tcctttttat acagaattaa actattttat agttcgacaa tcttacctgt
    27961 ttcaaagtag acaacccatt cacagatatt tttagcatag tcaccgatac gctccaagta
    28021 ggaaataact tggaaataat cacgacccgt aacaatggct tctggatttt tcttaatctc
    28081 ttcagtcgca aggtcacgga tagtttcaaa atagtggtta atttgctcat ccatggaggc
    28141 cacccggtat gcgtcgtcaa cagaaccatt aagataaaga tcaagtgctg cttccacaac
    28201 gcttttaact tcacgtccca tttttttaat ttcttcctct acagctggga tgcgttcttc
    28261 ccccttcata cggatggttg cctgggcaat ggctacagcg tgatccccca tacgctccac
    28321 atctgataca gccttaagga cagtcaagac tgtacgcaaa tcttgagaga ctggttgttg
    28381 gagtgcgatc atttcaaatg atttcttttc cagtttcact tcgtattcat ttacttctgc
    28441 atcatcttcg atgacctctt ttgccaggtc acggtcatgc gtgacaaaag cacgtaccgt
    28501 acgattgatt tgtgagagca cttcttgtcc catagcgtag aactggttat gtaatttctc
    28561 taaatcttct tcaaattgag atcgtaacat ctttcatctc cttatccaaa ttttcctgta
    28621 atatagtctt ccgtttcctt gtgttgggga tcaaggaaca tctgcttggt atcattaaat
    28681 tcaatcaaat ctccatctag gaaaaatcct gtcttatcag agatacgtga agcttgctgc
    28741 atggaacggg ttaccagaag catggtgtac ttgtctttta gaccatacaa ggtttcctca
    28801 attttaccag ctgaaatcgg atccaaagcc gaagttggct catccaagag gatgatttta
    28861 ggactagttg ccaagacacg ggccacgcag acacgctgct gttgaccacc tgacaatcca
    28921 atagctgaat catatagacg atccttgacc tcatcccaga tagaggcacc ttgcaaggct
    28981 ttttctacgg cttcatccag aacctgctta tccttaattc cattgatacg aagcccgtag
    29041 acaacattct catagatagt catagggaaa ggattaggtt gttggaaaac cattccgatt
    29101 tccttacgta attcaaccgt atctgtacgc ggactgtaga tgttgtgacc attgtacacc
    29161 acggatccag ttgtggtcac ctctggattg agatctccca tgcggttgag agacttgagg
    29221 agggttgact tccctgatcc agatggacca atcaaggctg taatttcctt aggttggaaa
    29281 gatagggaaa cactattcaa agccttcttt ttattataat atacggacag gtctgatacc
    29341 tgtaaaatcg catctgtcat acggtttcct ttctaaccaa agtgaccaga tacatagtca
    29401 ttggtggact gtagcttggc attttggaaa atagttgcag tcttgtcata ctcaatcaaa
    29461 tcacccaagt aaaagaagcc tgtatagtca cttgcacgag cagcctgctg catattatgc
    29521 gttacaatga tgatggtaaa gtttttcttg agctcaaaca tggtctcttc tagttgcatg
    29581 gtcgcaatcg gatccaaggc tgaggctggc tcatccatta agaggatatc tggcttaaca
    29641 gagatggcac gagcgataca gagacgttgt tgctgaccac ctgataaggt caaggctgac
    29701 ttgtggagat cgtctttaac ctgatcccag agggcagcct gacgaaggga ggtttctacg
    29761 atttcatcta ggacttgctt atccttaact ccagcacgtt catgcgcaaa ggtaatatta
    29821 cggtaaattg acttagcaaa tggattgggg cgttgaaaaa ccattccaat gtgtttacgc
    29881 atttcataaa cgttgatttc tggacggttg acatcaattc cacgatagag aatctgccca
    29941 gttactttag caatatcaat agtatcattc atgcgattga gactgcgtaa gtaggtagat
    30001 ttccccgatc ccgacggacc aatcaaagct gtaattttat ttctttcaaa ttgcatatca
    30061 atccccttaa tggattcatt tttaccatag taaacatgga catccttagt agaaagggct
    30121 actttttctt caggaaaggt aaggatatgc ttctcatccc agttatatgt tgacatggct
    30181 tctcctttag gcagcggtta atttcttgtg tagatagctt ccgaacttac gagctccaaa
    30241 gttaaaaatc aggataaaga tcaggagcac agcggcagaa cctgctgata caatggttcc
    30301 atctggaata gtgccttcac tattgacttt ccagatatgg acagccaagg tttctgcttg
    30361 acggaagata gagatggggc tagtcacact gaggatattc cagttagacc agtcaagagc
    30421 tggcgccgat tgccctgctg tatagatcag agctgcagct tcgccaaaga tacgaccaga
    30481 tgccaagacg acacccgtta caatacctgg aagcgcttct ggaataacaa catgaaccac
    30541 tgtctcccag cgagaaatcc caagagccag accagcctca cgttgggtat ggtgaacgtg
    30601 tttcaaacta tcctctacat tacgcgtcat ctgaggcaag ttaaagactg tcaaggccaa
    30661 ggcacctgaa atgattgaaa atccatactc aaactggact acaaagatca agtaaccaaa
    30721 gagacccacc accactgatg gtaaagagga caagatttca atacaagtcc gcacaaagtt
    30781 ggtaacagga ccttttttag catattcagc caagtaaatc ccagctccca tagaaagagg
    30841 tacagaaata atcaaggtaa tgaccaatag gaaaaaggaa ttgtaaagct gaatgccaat
    30901 cccaccacct gcttgaaaag cagaagacct tccagtcaag aaagaccaag agatatgggg
    30961 caagccccga accaagatat agagaatcaa ggaagccaag attgtcacaa tgatgctagc
    31021 aatcgtatag aggacagctg ttgcaagttt atctaatttc ttagcgcgca taatttttct
    31081 ttcctctttc tttcgtaatc aatttaatca cactgttaaa aactaaactc atcaagagca
    31141 gtaccaaggc cagtgaccag agaacattat tatttacagt tcccatgaca gtgttcccaa
    31201 ttcccatagt taatatagaa gttaaagttg cagctggtgt ggtcaaggaa gttgggataa
    31261 cagctgagtt tccgacaacc atctggatag ctaaagcctc accaaaggca cgcgccatcc
    31321 caaagaccac tgcagtgaaa ataccagaac gggccgcctt caagatcaca cgccagatag
    31381 tctgccagcg agtggctccc atagcgaaac ttgcctcacg ataataacga ggaaccgcac
    31441 gcaagctatc cgttgtcata aaggttacgg tcggcaaaat catgacaaag aggacggaaa
    31501 tccctgacaa aatcccaaaa ccagtcccac caaagacact gcgaacaaag ggaacgacga
    31561 cttgcaagcc aataaatccg tacactactg aaggaatccc aaccaggagt tcaatagctg
    31621 gttgcaaaat cttcgcccct tttggtgata cttcggtcat aaaaactgct gcaccaatag
    31681 caaagggtgt tgcgataagg gctgagagaa tggtaacgat aaaggaaccc aaaatcatag
    31741 gaagggcacc aaattcttta ctagaaggat tccaagttcc tcccaaaaga aagtcaaaga
    31801 tattcacacc attgacaaag aaggtcgaca agcctttttg cgctacgaaa accaaaatca
    31861 tggccacaag gatgactatc aaagaaagac aggcaaaggt caaacctttt cctaatttct
    31921 ccagacgaga attctttgat ggaagcaaca ttttcttagc taattcttct tgattcatta
    31981 ttgtctccct tccaacactg tcacagttcc ggcagcatct ttttcaacct tcatttcctt
    32041 aatcggaata tacttcaatc ctttgacaat cccttcttgg gtctcatccg agagaacaaa
    32101 attgagaaat tctgcagcta actcattggg ctgccccaat gtatacatat gctcataaga
    32161 ccacaagggc caattattgc tacttatatt ttctggactt aagtcatagc cattcaactt
    32221 catgcttttg accgaatcat ctatataggt aagagataaa taagagatag ctcctggact
    32281 ttttgatacg attgatttta ccgctccatt tgaatcctgc tcctgacttt gcatggcaga
    32341 ctgaccttcc ataatgacag tatcaaaggt agcacgagag ccagagccgg ctgcccgatt
    32401 gataacagag atgggtaagt ccttaccacc aacctctttc caattggtta cctcacctat
    32461 gaagatttga cgaagttgct ctgtcgttag gttatcaaca tcaacctcct tattgacaat
    32521 cagagccaag ccagctaccg cgaccttgtg gtcaacaaga gcagaagcat caattccgtc
    32581 tttttcctca gcaaatacat ctgagtttcc tatatcaact gccccagact gaacctggga
    32641 caagcctgta ccagaacctc ccccttggac attgaccgtt tttccaacat ggatcgtgcc
    32701 aaattcatct gccgctactt caaccaaggg ttgcaaggca gttgagccaa cagccgttat
    32761 ggattctcca cgatcaatcc agctagcaca gcctactaaa caagccgtca gccaaaaagc
    32821 gataagagac agagcaagct tttttctttt tttcactgtt tttctcctcg aaaataatta
    32881 tgaatactgt gaatttttta agtagttctt tatgagttga cgcatgaatt cttaccaaat
    32941 ttctgcgcaa ttgattattt atataatata ggctatatta ctctttccta acctcctttt
    33001 ttcatatgtg gataaaatct cttgtctatc ccttccccca ttgtcaccca ttatagtcat
    33061 ttcgtgtctc tttttcccct ttttaatgca agggaaatta ctctccttag atgataatcc
    33121 aaaagctaga aaggtatctc aaatctctct actctcccag actagtttac aactaaaagg
    33181 aaaagattct attttatgag aaatctagtt tacaagcggt aagaacgcta ataactaaac
    33241 ttcttgtact ctttgaaaat ctcttcaaag cagtgttttg agctatctat ggctagcttc
    33301 ctagtttgct ctttgatttt catcgagtag taaaactaca tgtgatggca atcaagatat
    33361 caagaatcat cctactaaaa aaatccatac tttcactata acatagaata agatatttga
    33421 ctagcatttt catttgaatc tgaggccttt tggaaaataa tttttcaaaa catttccagt
    33481 aacctttgca aagcccaagc cattgccttt aaccaaaact tggtaccaac catttggcag
    33541 actttctgcc agctgaacgg tttctccagc cgcatacttg acaaactctt cttggccaat
    33601 ttcaaccgac tgttcgacct gactcggttt caaggctaaa ccaagagcga aactgggctc
    33661 aaagcgtttc ttcttaaaag tacccagatg cagtccattg cgagcaatct tgagcttccc
    33721 taaatctggc aaaagttctg gcaagagata aagctggtct ccaaaaatct gcaagatacc
    33781 cggtagattg accttcaaat ggttttgggc aaattcctgc cacaaggcaa cttgttcacg
    33841 gctgaggtta ctcttacttg ccttaaattt aggagctgga ttgttaccct taaactgt
//
LOCUS       DAVBHS010000006        31002 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVBHS010000006 DAVBHS010000000
VERSION     DAVBHS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31002
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.006"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(298..516)
                     /locus_tag="VQ651_000182"
     CDS             complement(298..516)
                     /locus_tag="VQ651_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2753236.1"
                     /translation="MVLNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(762..1040)
                     /locus_tag="VQ651_000183"
     CDS             complement(762..1040)
                     /locus_tag="VQ651_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753237.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEMLELACWFRVIIVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYSIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            complement(1254..2885)
                     /gene="rlmD"
                     /locus_tag="VQ651_000184"
     CDS             complement(1254..2885)
                     /gene="rlmD"
                     /locus_tag="VQ651_000184"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET2753238.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSLHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWCTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSQKNASLNKITNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2959..3933)
                     /locus_tag="VQ651_000185"
     CDS             complement(2959..3933)
                     /locus_tag="VQ651_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HET2753239.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(4007..4621)
                     /gene="pmp23"
                     /locus_tag="VQ651_000186"
     CDS             complement(4007..4621)
                     /gene="pmp23"
                     /locus_tag="VQ651_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HET2753240.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(4621..5595)
                     /locus_tag="VQ651_000187"
     CDS             complement(4621..5595)
                     /locus_tag="VQ651_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HET2753241.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQKENGTYSILIKNIEDIQSK"
     gene            complement(5603..6859)
                     /locus_tag="VQ651_000188"
     CDS             complement(5603..6859)
                     /locus_tag="VQ651_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET2753242.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6921..7352)
                     /locus_tag="VQ651_000189"
     CDS             complement(6921..7352)
                     /locus_tag="VQ651_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2753243.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(7354..7956)
                     /locus_tag="VQ651_000190"
     CDS             complement(7354..7956)
                     /locus_tag="VQ651_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HET2753244.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7940..8779)
                     /gene="prmC"
                     /locus_tag="VQ651_000191"
     CDS             complement(7940..8779)
                     /gene="prmC"
                     /locus_tag="VQ651_000191"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET2753245.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(8779..9858)
                     /gene="prfA"
                     /locus_tag="VQ651_000192"
     CDS             complement(8779..9858)
                     /gene="prfA"
                     /locus_tag="VQ651_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET2753246.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9855..10445)
                     /locus_tag="VQ651_000193"
     CDS             complement(9855..10445)
                     /locus_tag="VQ651_000193"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET2753247.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRGLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            10568..10750
                     /locus_tag="VQ651_000194"
     CDS             10568..10750
                     /locus_tag="VQ651_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HET2753248.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10887..12260
                     /gene="mnmE"
                     /locus_tag="VQ651_000195"
     CDS             10887..12260
                     /gene="mnmE"
                     /locus_tag="VQ651_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET2753249.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEANLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLIEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(12307..13242)
                     /gene="dapA"
                     /locus_tag="VQ651_000196"
     CDS             complement(12307..13242)
                     /gene="dapA"
                     /locus_tag="VQ651_000196"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET2753250.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATITGVLRPDY"
     gene            complement(13298..14374)
                     /locus_tag="VQ651_000197"
     CDS             complement(13298..14374)
                     /locus_tag="VQ651_000197"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET2753251.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14716..14788)
                     /locus_tag="VQ651_000198"
     tRNA            complement(14716..14788)
                     /locus_tag="VQ651_000198"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14753..14755),aa:Thr,seq:ggt)
     gene            complement(14888..15397)
                     /locus_tag="VQ651_000199"
     CDS             complement(14888..15397)
                     /locus_tag="VQ651_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HET2753252.1"
                     /translation="MKKLTIRDIADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15395..15491)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15520..15951
                     /locus_tag="VQ651_000200"
     CDS             15520..15951
                     /locus_tag="VQ651_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET2753253.1"
                     /translation="MKIPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKAYIFRK"
     gene            16033..16410
                     /gene="mscL"
                     /locus_tag="VQ651_000201"
     CDS             16033..16410
                     /gene="mscL"
                     /locus_tag="VQ651_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET2753254.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16452..17546)
                     /gene="hemH"
                     /locus_tag="VQ651_000202"
     CDS             complement(16452..17546)
                     /gene="hemH"
                     /locus_tag="VQ651_000202"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET2753255.1"
                     /translation="MKKAILMMTFGSPEEISFEGVAEFFTNIRRGVRPQDHEIQTLYD
                     NYIRIGGTPLQRITREEVNLVKERLGEEYGIYFANKFSRPFIPDVIKQMETDGVEECI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEVLLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            complement(17624..18456)
                     /gene="srtG2"
                     /locus_tag="VQ651_000203"
                     /pseudo
     CDS             complement(17624..18456)
                     /gene="srtG2"
                     /locus_tag="VQ651_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218039.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG2"
     gene            complement(18534..19532)
                     /gene="srtG1"
                     /locus_tag="VQ651_000204"
     CDS             complement(18534..19532)
                     /gene="srtG1"
                     /locus_tag="VQ651_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG1"
                     /protein_id="HET2753256.1"
                     /translation="MKTKREKPKKSLSRRLVLAVDGVINHLLLIFAALIFLFGFYALW
                     DSNQVYSLASSSEYEAYRPVTTQQDELASFSGFSKLQELNPEVLGWINVYGTNIDYPL
                     VQAKDNEKYLNKDSKGEFAATGAIFLDARNNPKFEDFNTIIYGHHVENGVMFGDVAKF
                     ADQEFFDQHRYGSIYYNGVEKGLEIFEMLEVDAYDFNIYDPGIQGEDRQQAYLDHLLS
                     VAMHKRDISLSPSDRIILLSTCFLDVTNGRHIVVAKITDTVPKNTFHTKKSKPFPYSV
                     FDDSSLGRFLSSIPLWIWYLILFVLFLLLIFLLLVLYLILRRRRESKKNARSRPFY"
     gene            complement(19550..20746)
                     /gene="pitB"
                     /locus_tag="VQ651_000205"
     CDS             complement(19550..20746)
                     /gene="pitB"
                     /locus_tag="VQ651_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus major pilin PitB"
                     /protein_id="HET2753257.1"
                     /translation="MKKTFFKKLFTASIAAITALSVFRGVPTFADDNSAITKANGENN
                     AVVKINKTLNIAEGITTPTATFTFKFTEKTGQSSNGAPYQTGVAIPDRNVEYNKNDHP
                     TADKIQKATEDIFSGVAYGHAGEYVYDVAEAKTGWQAITKNGKTIDAMRYDKRTYEMH
                     VIVKNKVNGGVYISSVYFKENNKSNAPKVEPSEQGVYNLFDNTYTKDASKEPNPDDPS
                     QVDPNAKALTITKKVDGASGDKTRDFQFHIKIQLPSTNKTAETPVTNIIVKHGSKSEV
                     LAVVTPADTVEYNFTLKDGETFTVEQLPAGSKYTVTETGVAGYTDSSIYTTNGAEQTS
                     QGQKNVDFTLTDILIGEKKNDNKVTNKIDDVTPTGLLIDNLPFILMIGLGLAGFVVLS
                     KKRREA"
     gene            complement(20791..21342)
                     /gene="sipA"
                     /locus_tag="VQ651_000206"
     CDS             complement(20791..21342)
                     /gene="sipA"
                     /locus_tag="VQ651_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system signal peptidase SipA"
                     /protein_id="HET2753258.1"
                     /translation="MLLKKKHKKPVTQVNRDKSPPSVWGDILYLVSKLLMVGFVLAIL
                     YFFVFGLLRYNDDGMKPALKDGDLVVYYRLDKRYSIGDLLVYSYKGKERVARVIATEG
                     STIDINENGLIINGSPQQEQDIYKETLLYKEGATFPMKVPAGQLFVLGDNRTTAVDSR
                     AFGTIPIQDTQGKVVTVIRRRGF"
     gene            complement(21342..23105)
                     /locus_tag="VQ651_000207"
     CDS             complement(21342..23105)
                     /locus_tag="VQ651_000207"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753259.1"
                     /translation="MIDTPYWFTSGLERGMLNISSLIAPKIDGFYSIKFRYYIGSNDS
                     ITSLKGYISGYNSGIPNEIFSANNENDLQQKFKEITDKILPLGVHHVTISDVLSKYVQ
                     LLPGDASHLRVVKIKDGNEQELNDNQVTIETKKNEQGLVEVTAKFNPSYTLEDDAKYV
                     LKFTVTSSQEAFDAIAGDKTLTSDDAEEADATKLYSNKGAKVAYSYGIGTSRTKIKDY
                     SEKPTFKPSDPLTVPVEIEWKGVDGKSNPSANRPPSVELNLNQKKDGSIKDSYRKVTS
                     PVQTNSFTENTSFAKVAKGYDYELKAPDAPGYTVEVQKTGTKEKPSFKVIYRQLPSLT
                     VKKILEGEQSPNKSFTINVTFSDKDGKPINGKFGNTTVTNGKAQISLKNSQETALSYL
                     PRDTHYKVEEVENSRTGYHVTYEKQEGTLSEDVQTIVTNHRLPTLSVTKKVTGAFANL
                     LQSFKITINVKDAQNKPLNGSYSAIVNNQKTTLQFTNGKATVDLKKDKTIKILDLPLN
                     ARYSIEEEASSSRGYQVSYDKKEGTLDANKSATVTNNKNSVPETGIDFLSSTLVLGVV
                     LPLGGIFFIILLGHLVVNRRK"
     gene            complement(23427..23930)
                     /locus_tag="VQ651_000208"
     CDS             complement(23427..23930)
                     /locus_tag="VQ651_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HET2753260.1"
                     /translation="MNVQYDFKKIQYFTSSLVIFLAILFLCAPINSLRADSITEPQTT
                     LHKTITPISGQKDQYELSLDITSKLGTETQSEPLDVVLVADLSGSMEERDVWSYSSRR
                     YISRIEALKHTLKGVNGRQGLIDTILSNSQNRLSIVGFAGKIDNQYNDRYYNEYYLSY
                     QYGTWPN"
     gene            24289..25512
                     /gene="pepT"
                     /locus_tag="VQ651_000209"
     CDS             24289..25512
                     /gene="pepT"
                     /locus_tag="VQ651_000209"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HET2753261.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPRQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(25548..26177)
                     /locus_tag="VQ651_000210"
     CDS             complement(25548..26177)
                     /locus_tag="VQ651_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753262.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSANSKDKTALLESTKAILVLNHQIQSKVSASEETSPARNAEANSDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            26142..26363
                     /locus_tag="VQ651_000211"
     CDS             26142..26363
                     /locus_tag="VQ651_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753263.1"
                     /translation="MDLFQELICLVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(26634..26982)
                     /locus_tag="VQ651_000212"
                     /pseudo
     CDS             complement(26634..26982)
                     /locus_tag="VQ651_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(27087..27647)
                     /locus_tag="VQ651_000213"
                     /pseudo
     CDS             complement(27087..27647)
                     /locus_tag="VQ651_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(27873..30992)
                     /gene="phtE"
                     /locus_tag="VQ651_000214"
     CDS             complement(27873..30992)
                     /gene="phtE"
                     /locus_tag="VQ651_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HET2753264.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQISQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         8835 a         6359 c         5686 g        10122 t
ORIGIN      
        1 aaaaggatct agtttttatt tttagttaaa ttatgtctat atttttcatt aactcatcaa
       61 taaaaatttg tttgctaata tctctttctt ctgattttgt ttcaaggatt attgctacat
      121 cattcatatt tttaggtaaa taccaaccat ctggcttatc tgaatatccc ttgaagccta
      181 attgattaaa agtagttatt tgaccagtac cttgattgat gttttcttca acttcattaa
      241 agcctatact tctcttgcat aatccctaac ttgatcttct gtccttaaag tcattttcta
      301 ttcctcgtca ctaaaattat cttcaaaact aataatatca ttaggagtgc aagataaagc
      361 cttacatatt ctttctaaat tcttaaatgt aattggttta tcctttccca tttgtgccat
      421 aacatttgtt gtaagtcctg ccatagctat tacatctgtt ttctttagtc ctttttttgc
      481 taactgtatc cataatggtt tatagttaag taccataata tccctctttt tttcttcatt
      541 aaatttattt aattatagca taaatattga tttcattcaa tcttacattg atttagtgat
      601 ttgttttaga gaatttgttg tgttattccg tccttatttt gccataaatt gttttaagtg
      661 ggacctctta tccttaccat ttttacctcc taattttgag tataaaaaag acgatagagt
      721 ttttaatttc tatcgtctga gatttacata ttaaattttc atcacaaaat aagactcttt
      781 aaagaatctt taaattcatt ggtatgctgc ttacaatatc cattattaaa atggatacta
      841 taagcagcac accaactatt tattttttga gttttaaact ttggtagaag tcttgaactc
      901 cttatattta cataactatt tgcctctata actattatga cacgaaacca acaagcaagt
      961 tcaagcatct ccatatcttt attcaaatga ggactaagat attcacaaac tttatcataa
     1021 acctctttat cttttttcat aaaaggggca tacaacttag caaatttatc ctaaaatttt
     1081 ttgttgcctt tatcaatcat ttcaacgctc ctttaaatat aaaaataatc ctatcaatct
     1141 ttatattgag cctattttct aattgatacc ttgcaagaaa ggaataatag ctgttcctat
     1201 accaagtagg aatgtaataa atgcagaaag tcatatactg tcattctttt ctattaaatc
     1261 attttgaagt gtctcaaagc atccatgatg gcttcttctt tttcaggtgt acactgtgga
     1321 ataatttgtt tatccttttt agacttgttg taatgttctc gtaattctat tccacatttc
     1381 ttttttatct gtgcaatata taatgtcgaa acttttaatt caaatttatt ccaaacatat
     1441 tctttgattt gagcatatgt tgctttgctc tccgcacttg tcaaatccat ctcatccagc
     1501 tcaatttcaa cacttatgtg cttatcgaca tcgagtttgg acaaaagtgc taccgtctcg
     1561 acgtgatgcg tttgaggaaa tagatccacc ggctggactt tcttcaattc atatcccaat
     1621 tcttggtaga gtttgatatc acgcgccatg gttgcgacat tgcaggagat ataggcgatg
     1681 cgatcggctc ctgtttgggc gcttgctttg ataaagcttt ctgtcaagcc cttgcgtgga
     1741 ggatcaacca agataacggt tggttgaata ccttccttga gccatttctt catggcattt
     1801 tcagccgtgt cacagacata gtgggcatta gtaatcttgt tcaaagaagc attcttctgg
     1861 ctattctcaa ccgcttctgg aatcagttca acaccgtaga cttctttgac atgcttggcg
     1921 actgataaac caatggttcc aataccagaa taagcatcaa taaccacgtc atcttttttt
     1981 aactctgcaa agtcaatggc tgtttgatag agtttctccg ccatttcagt attgacttgg
     2041 taaaaggctg ggccagcgat ttggaagtca tttcccaaca tctggtccgt aatatagtct
     2101 tgaccataaa gagtgcacca ctccttacca aaaatcgcat tggtattctg gtcgttgata
     2161 ttttgcatga cagacacaat ctctgggaac tgcttgataa cttgttcaat caattggtca
     2221 acacgaaaaa cttttggacg agttgtcacc aaaacgacca tgatttgtcc tgaatagtga
     2281 ccacgacgca ccacaagatt ccgaatcaat ccagactgtt ccttttcgtc ataaggtttt
     2341 aaatcaaaac gacggagcag gtctcgaaga gctactacga cttggtcaat gacaggatcc
     2401 tggataaaga aatcttcaag gggcatgagg ttgtgcgaat tcttacggaa aaatcctgtt
     2461 tccaagacac cattcactcg acgaacgggc acctgcgcct tattgcgata cttgactgga
     2521 tgttccatac caagcgtttc agcaacttct acatctgcaa ttccagcaat cttgtagaga
     2581 ctgtccttga cttgcttggt tttaaacttg agctgttctg gataagaaag gtgtcctaaa
     2641 tccgcgattc ctgaacgcag gtaagccaaa tctagatctt gattacggtg tagtgactgg
     2701 acaaggtatt tttcaacttt tccaaagcca atctttttat tgaccttgag gacacgcatg
     2761 agaatttttt cactcggtaa agcattctct acaaaaaaga ccaaaccatc taccttggca
     2821 actcctgccc cttcatgggt caaatcaaca atttcaactt ctacaatatc atttttcttt
     2881 aacattcttt tctctttcta ttggccgaca aaaaagacgg caacgttacg attaccatcc
     2941 attataccat gaaatttctt aagaaccaaa actcttgaag aagttgacaa gggcttgcca
     3001 gagggagctt aagaagttac caccgtcctc tagcgcctta tccgcatcaa agttaaggtt
     3061 gatattttta aaactgtcgc cagcttgtga tacgatgctt tgtttaaggt catttagggt
     3121 tttagtgaaa tctgcattgc tgaggatatc actctttgag agattcaagg caaaattgat
     3181 gatgatattg atctggtttc ctgttatgac ctgatcaagt ttgtaatttt ttaaggtatc
     3241 ttcaacaatc ttgcggacat cttcttctgt cagatttccc ttgctttctt tagctttggc
     3301 gagtcctgac ttgatatcag ctagggcaac gtttaattta ttagcatcat agcctgattt
     3361 gtccttgttt tcagcattga tatctgacaa agcctttagc tcttcttgag ccaaatcttt
     3421 attggcttgc ggcaccttgg ctccattagc ttctagcgaa tagtagatcc cagccaaagc
     3481 actctcacct gtaactggaa taggggctgc tacagtgatt ttggcatgtt ccatacccag
     3541 cgttactgct gcgtttcggt acatatcctg agtcacctta gtgatatttt ctggtgtttc
     3601 aatcttgacc tcaagtggcg atttgtcacc tagcttttga atcttggctg atgaatacaa
     3661 ctgtaagcta gagtcattgg ccacattcat gattttagaa taaacatcag gtgtcatggt
     3721 cttgagttct ttggtatctg ttgaggcatt gtagcccagt tttttaagag tttgattttt
     3781 ttggtcttca gatagggagg aacctaggac atattcaggt tggacatagg tttcatcgat
     3841 aaccttttga acatctgttg ctgcatggac gctattcata gctgttactg cccacaagac
     3901 cgcagcactg gtcagaaaga gtttctttct catagggaat ttcctccttt acttttctag
     3961 agtaatatat ctatcttaaa gaaaacttat aacaaaaaca cctggtctag ccagatgttg
     4021 aaaagagagt gaaacatttg atgatgtaaa ggttaagtcg tacctgtcta gaataataat
     4081 agtttcctcc atttacatag agttcagcac cgtgaaaaat ggaaatgggg tgaatataac
     4141 tataagtctt tccagtcgta ttaccaagca agggggcaac agtctcacga gagtactgtt
     4201 tggctagagc caaggtattt tccttgccat tttgggcgat aaaatcgata taggcaggtc
     4261 caaaattata ggcttgaaca gctgtccaga tatctacccc cttcttctgc gccagataga
     4321 gattgcctgt cagagtttga atgccttgcc gaatgctaga ggcattatca ttgatggtgt
     4381 tggttgaacc acttgcagac tcactagact gcataacatc gccttctttt ccttttgttt
     4441 cagtataaat catagcaagc acaagctctt cgtttgctgg ggtgtctttt tcactcaaga
     4501 tttctcgcac catgggttga taggtcatga cttgtttgac atcttgatga acgcggtaag
     4561 ctttatagcc agcaaaaagg aagactgcta gtacaagcac tcttcgaatt cgtttaaaca
     4621 ttatttactt tggatatcct cgatattttt gattaagata gagtaggttc cattttcctt
     4681 ttggataaac tcaacagact cggcgtcttg atagacgtta ttgggaacga tgagctcaat
     4741 tccatttgat aaggagagtt tttggttttc aaatttcttt aattggcgac tggcatcaat
     4801 ttcatcaaat tgaacaggtt ctggtacggc ttctttgact tggtcaataa agctcaaacg
     4861 agccgtcaga ttgttgtcaa aaaggtcatt agccaatttc tcaggtgaca attcattgct
     4921 ttcttctagg ttgttgaaaa tcgctgattt gaccttggat tgaaattgaa aatcatctgt
     4981 gttaaaagat tcagcaattc tctgggctgt tttttccagt tccttgatag attttttagg
     5041 agaaatctta ggagcgacag caagaagatt atctgaaaaa tagttcaaaa aagtcccgtt
     5101 gtacttgatt cgtttttcaa tcaggtgata cttgcgactc tgaagattga ccaccaaggc
     5161 ctcgtcagca cccgttccaa atccaggcag gttattctga gtcagcttga ttggattatc
     5221 aacttctcct ccgaggtggg tcaaggtctc ccgcagggca attcgcaaga aagcgaaatg
     5281 ttctacacct tctttagaaa attgaacaaa aatcaagtca ttggtcttga gattttcaga
     5341 aatgctaaac tcctctttcc agagattagc cagcgttact gatgtctcca acaaatcgtc
     5401 tgtaatatga ttgaagaagg gattttcttc ttcgaaaatc ccagtcttgg cttcatctga
     5461 atacacatgt tcaatttttt tacgtaggta ttcttcgatt tttggagtaa tattgagaaa
     5521 cttatctgct aagaacagct cggtatcatc cggactgaac tggtgaataa tggctttctt
     5581 aatataaatg tccataaaag ttttattcct cgtataatgg gaaggcatct gtcaattctt
     5641 tgactgcact tctcacttct tctaatacag cctcattttc tgaattctta agggttttaa
     5701 tgatgagttc agccactttg cgactttctt cttcaccaaa tccacgtgca gtaatagctg
     5761 ctgctccgat acgaatccca cttgtcttga atggtgacaa ggtttcgtaa gggattgaat
     5821 ttttatttag ggtaatattg acttcatcca gcaagttttg agcaactttt ccgttttcta
     5881 caaccttagt cacatcaaca aggaagagat ggttttcagt tccacctgaa ataatacgga
     5941 aatcagggtc ttgcaagaag acatctgcca tagccttgct gttcttaatt acattggcag
     6001 catattcctt gaaggctgga tccaaaactt ctttgaagga aactgcctta gccgccacaa
     6061 catgctctaa aggaccgccc tgaatacctg ggaaaatagc tgaattgatt tttttagcaa
     6121 gttcttcgtc attggtcaaa atcaaaccac cacgaggtcc acgaagggtt ttgtgggtcg
     6181 ttgttgtagt gatatgagcg tatggcactg ggcttgggtg aagaccagct gcaaccaagc
     6241 cagcgatgtg ggccatatca accataagct tcgcaccgac agcatctgcg atttcacgga
     6301 attttgaaaa gtcaataatt tgagaatagg ctgaagcacc agctacaatc agttttggtt
     6361 ttacttcttg ggcttgtttc aagatagcat caaagtctaa gagttccgtt tcaggatcaa
     6421 cactataaga aacaaagttg taggtttgac cagagaagct aacaggagcc ccatgagtca
     6481 aatgaccacc tgatgccaaa tccattccca taaccgtatc acctggctca atcaaggaca
     6541 tgtaagccgc acagttggcc tgacttcctg agtgaggttg gacattggca aatttagcac
     6601 cgaaaatttc ttttgcgcgt tcaatagcaa gagtctctac aacgtctact acatcagttc
     6661 caccataata acggcgtcct gggtaaccct cggcatattt atttgtcaag atagaccctt
     6721 gagctgccat aacagccttg gaaactacgt tttccgaagc aattaactcg atattatttt
     6781 gttggcgttc ttcttctttg gcaatagcat tccagagatc agcatcatat gctttaaaat
     6841 catctttgtc aaaaatcata ggtcttctcc tttattgtgt gactagtcca ttagtttgat
     6901 tttacaataa gaaaatcaaa ctaaaagatg cgaataaacc gtttctgcat tttatcacaa
     6961 gtatagccaa ctttttcata aaatgcatga gcacccagac gatgattggc agaatttaag
     7021 cggataaacc cataaccaca tcttttggct tcttgttcca acccttgtag taaactttta
     7081 ccgatacctt gaccttgcgc ttgaggtgaa actgctaaag ctaagatatt aaatcctgct
     7141 ttggaataga gtgattcgta aacttcagcg tggacatatc caagtaagac atgattagct
     7201 gcatcctcat agccaagtag gaaatgatgg gaatcctgag acagtctagc tagttggcta
     7261 gccgtttcct ctggactaaa agtataaccc aaagcctctt ggttgatgtc acatatcgct
     7321 ttcacatctg tttcttgcaa atctcttagc atttcaagcc tcctcaaaag aaatctctgg
     7381 caaccgagca agaatatctt ctcgtttaat tgcgccttgg cgtaagattt tcaccttgtc
     7441 tccagacaaa tccacaatag ttgaatcctg tccagttaga aaagcatcgt cttccagacc
     7501 cagaacctct tggtcaaaat ccttcagaat ttgttcaaag gttacaccac ttgcctgacc
     7561 tgagatattg gcagacggcc caatcaaggg acctgtctct cgaattaaat ccagtgtgat
     7621 agggtgactg ggcatccgaa atccaatagt tgcaaggtca gaatttaccc aatagggaac
     7681 tcggtcattg gcttcgagaa taatggtcaa gggacctggc aaaaaggtct ctacaagttt
     7741 ttgtagataa gttggctgat tcttagaaaa gtgcaagata tcctcgaaag aggcgatatt
     7801 gagattgagt gccttatctc taggacgacg tttgagttgg taaacatggt caactgcttt
     7861 ttcatctaag gccttggcaa aaagaccata aacagtctct gtaggtagaa cgacagctcc
     7921 accattttcc aactcttgtc taatcctgtc catcatcaac cacaaccatc ctattttgac
     7981 caaattggtc tttgagtgtt cgaacttttt tttcaggaag atgttttcta aaaagttcgg
     8041 gaacactttg accttgcttg tatccaattt caaggtaaat cttaccacca tccttgagat
     8101 agtcttttgc atcttccgca attctacagt aaatagctag gccatcctcg tctgcaaaga
     8161 gagctagatg aggctccgaa tgcaaaacat tcaagcctac ctctgactca tcttcacgag
     8221 agatataggg tggattggat acaattatat catatttttc agaaatttct gcaaaacaat
     8281 cagatttttt aaaaaatata ttaagatttt gatcgctagc attctctgat gcaagctcta
     8341 aggcctcttg agaaacatct gctgctgtca ctgaccaatc tggtctattt tttgctagag
     8401 cgagagcaat agctccacta cctgttccaa tatccagaac tgagagattc gtctcaagat
     8461 tttcagttag aataagctcc actaactcct ctgtttctgg acgaggaatc aaaacccgtt
     8521 catccacctt taaatgcatt ccataaaaat ctgcctgtcc aatgatgtac tgagctggct
     8581 tgtgagctgc tagttgctgg taaatatctt ctacaaattg tttttcttcc tctgttgtca
     8641 cctcctgctg gagggcaaaa ataaagtctg taaaagatag atttttcaga ctacgataga
     8701 caaaagagag gctttccgct tcctctcctt gtcttatcaa ctcttcttca aaatttgaaa
     8761 ataattgagc taatttcatt atttgtttaa ttcttctagt ttttgtgttt ggtcataaag
     8821 caccaaggca tccacaactt cgtccaattt accagacaaa atcgtatcta gtttttggag
     8881 ggtcaagccg atacggtggt ctgtgacacg gttttgaggg aagttataag ttcggatccg
     8941 ttctgaacgg tcaccagtac cgattgtcga cttacgctca gcgtcttgtt catcctgagc
     9001 aatctgagca aagtggtcag cgacgcgtgc acggataatc ttcatagcct tctcacggtt
     9061 cttctgctgg gtacgttctt cctgcatctc aaccttgata ttggttggca agtgaacgat
     9121 acgaacggca gtcgcaacct tattgacgtt ctgtccacca gcacctgatg cgtggtaaat
     9181 atcaacacga agatcttttg gatcgatatc gtattctact tcttcgactt caggcatgac
     9241 aagaactgtg gcagttgagg tatgaacacg accttggctt tctgtcacgg ggacacgctg
     9301 cacacggtgg gcacctgatt cgtacttaag cttagagtat acagactgac ctgaaaccat
     9361 agcaaccact tctttaaacc caccgacacc attcatagag gcttccatga cttcaaagcg
     9421 ccagccttgg gcttccgcat acttttggta catagttagc aaatctccag cgaaaagtgc
     9481 cgcttcgtct ccaccagctg ctccacggat ttcaaggatg atattcttgt catcgtttgg
     9541 atcctttgga aggagcaaaa ttttcagttt ttcttcatat tcttcttttt cagccttggc
     9601 atctttgagt tcttgcttgg ccatttcttc caagtccgca tctccgcctg attccttaat
     9661 catctcttcg gcatcgacga tattttgaag gacttgttta tactcacggt aggctattac
     9721 ggtatcacga ttggaagctt cttcttttga aagctccata aaacgcttgg tgtctgaaac
     9781 gacatcaggg tcactcagca attctcctag ttcttcataa cggtcttcta cagcttgtag
     9841 ttgatcatag atgttcattt ttcttcattc tccttattga tttcaggggc aaaataatgt
     9901 ttacggcaaa ccgagatata ggtttcatta ccaccaattt ggatctgttc tccatcgtaa
     9961 acgggcagtc catcctgtgt tcgcaacacc atggtcgcct ttttcttgca atactgacag
    10021 atggtcttga tttcgtcaat cttgtctgct aaaagcaaga gatatttgga accttcgaac
    10081 aattcattgc gaaagtcatt tttcaagcca aaagccatga cgggtatgtc taactcgtcc
    10141 acaacacgag ctaggtcata aacatggtgg cgtttgagaa actgggcttc atcgaccaac
    10201 acacagtaag gtttttctgg taggcctcgg atatagccaa agatatccgt tgtttcctca
    10261 atcgcaaggg cagggcgttt catgccaatt cgactcgaca catagccaac accgtcacgc
    10321 gtatccagag ctgaagtcat aatcacaaca ccttttcctt gctcctcata gttataggcc
    10381 accttgagaa tctcgattgt ctttccagag ttcatggtcc cataacgata atacaactgt
    10441 gccatgtttc ttgcttcacg tccatttcta aatttttgct acattctagt atatcataat
    10501 tttcttaagc tttaaacggc aaaatgtggt aaaatggaag aaatcaaaac tagtggagga
    10561 agctattatg ccatttgtac gcatcgattt atttgaagga cgcacgctcg agcaaaagaa
    10621 agctcttgct aaggaagtaa cggaagcagt tgtccgcaac actggagccc ctcaatctgc
    10681 tgtccatgtc atcatcaacg acatgccaga aggaacttac ttcccacaag gggaaatgcg
    10741 tactaaataa gctagcttaa gcagaattgc ttaggctttt tcaatctcca agtagcattc
    10801 attgaagaaa tatcctaaat ttgttacaat ttgaaaagaa acttggagaa tttccaagaa
    10861 aagagctatt aattaaagga aacattatga ttacacgtga atttgatacc atcgctgcta
    10921 tctctactcc actaggtgaa ggggctattg gtattgtccg cctgagcgga acagacagtt
    10981 ttgctattgc gcaaaagatt tttaaaggaa aagacttgaa caaggttgcc agccacactc
    11041 tcaactacgg tcacattatt gatcctctga ctggtaaagt catggacgag gttatggttg
    11101 gggctatgaa gtctccaaag accttcactc gtgaggatat tatcgagatt aacacccacg
    11161 gtggtattgc ggtgaccaat gagattctcc agctagctat ccgtgaagga gctcggttgg
    11221 cagaacctgg tgaatttacc aaacgtgcct ttttaaacgg tcgcgtagat ttgacacagg
    11281 ccgaggcggt gatggatatc atccgcgcca agacagacaa ggccatgaac atcgcggtca
    11341 aacaacttga tggttccctt tctgatctta tcaataatat ccgtcaagaa atccttaaca
    11401 cacttgccca agttgaggtc aatatcgact atcctgagta tgacgatgtt gaggaagcca
    11461 ctactgctgt tgtccgagag aagacaatgg agtttgagca attactaacc aagctcctta
    11521 ggacagcacg tcgtggtaaa atccttcgtg aaggaatttc aacggctatc attggacgtc
    11581 ccaacgttgg gaaatcaagc cttctcaaca acctcttgcg tgaggacaag gctatcgtaa
    11641 ccgatatcgc tgggacaaca cgagatgtca tcgaagagta cgtcaacatc aatggtgtac
    11701 ctctcaaatt gattgataca gctggtattc gtgaaacgga tgatatagtg gaacaaatcg
    11761 gtgttgagcg ttcgaaaaaa gccctcaagg aagccaactt ggttctacta gtgctaaatg
    11821 ccagtgaacc actgactgcg caagacagac aacttcttga aattagccaa gataccaatc
    11881 gcattattct acttaataaa accgacctgc cagaaacgat tgaaacttcg aaactacctg
    11941 aagacgttat ccgtatttca gtccttaaaa accaaaacat cgacaagatt gaagagcgaa
    12001 tcaacaacct cttctttgaa aatgctggct tgattgagca agatgctact tacttgtcaa
    12061 acgcccgtca catttccctg attgaaaaag cagttgaaag cctacaagcc gttaatcaag
    12121 gtcttgagct ggggatgcca gttgatttgc ttcaagttga cttgactcgt acttgggaaa
    12181 tcctcggaga aatcactggg gatgctgctc cagatgaact catcacccaa ctctttagcc
    12241 aattctgttt aggaaaataa gaaaaatcca tgatccttca ttcggtcatg gattttattg
    12301 tctttattag taatctggtc ttaagacccc tgttatagtt gccttagttg cttcgtagtc
    12361 gccatctacg acaaccttga taatgcgttt ggcatcttct tctggtgctg gaacaagagg
    12421 tagacgagtg ggtccagctt caaatcccat atagttaaga attgccttaa ctggagcagg
    12481 acttggataa gagaagagag cattaacctt aggaatgaat ttacgctgaa ttgctgcggc
    12541 tttcttcata tcgctttctg caatggcagt aaacatctcg tgcatttcat ccccatttgt
    12601 atgagaggca acagaaataa ccccatccgc cccaaggttc atggcatgga aagcatctcc
    12661 atcctcacct gtataaatca agaactcttc aggcttgtgc tcaatcaagt aagccatatt
    12721 agccaagcta gtacattctt tgacaccgat aatatttgga tggtcagcca agcgaagcat
    12781 ggtttctgga gtcaattcga caactacacg ccctggaatg ttatagataa taattggtag
    12841 gtcagaagca tctgcaatag tcttaaagtg ctgatacatc ccttcttgag aaggtttgtt
    12901 gtagtaagga acaatagcaa gcccagctgc gaaaccacca aattccgcta cttctttgac
    12961 aaactcaata gagtcacgcg tatcattagt acctacaccc gcaatcaaag gaacgcgtcc
    13021 attgacaacc ttttgtacag ccgcaaacaa ctccaactcc tcatcgtggg tcaaagttgg
    13081 actctcagca gtcgttcctg cgagaagaat tccatccgta tgatgggcca ataaatgctc
    13141 aatcaaggct ggaatagcat caaagttaat agaaccatcc tcatgaaagg gggtaataaa
    13201 ggctgtaatg attttacatt cttttaaatc ttgataagac atgaaaacac ctctctattt
    13261 caaagaagga gttcatctga actcctaaac gatatgacta ttttaattca aatttcaatt
    13321 cggctgttgg acgaaccaat ccacgttcat gaagagtttc agcaatctga actgagttcc
    13381 aagcagcacc tttgagaagg ttatctgaaa caacccacat gtgaattcct ttttctgcat
    13441 ccaagtcttt acggatacga ccaacaaagg tatcacgcga accaactgca ttgatagctt
    13501 gaggatagat ttgatgagct acatcatctt caagaacagc acctgggaag gctgcgatag
    13561 ctgcttttac ttcttcgatt ggagccactt cttttgtttc gatataaaca gactcagagt
    13621 gagctgacaa gactggaata cgcacacatg ttgcagatac tgcaatgcta tcatcttcca
    13681 taattttctt agtttccttg gtcatcttca tctcttcgta cgtgtaatca ttatcagtga
    13741 aaacatcaat ttgtggaaga gcgttaaagg cgataggata atgtttcttg tcaccacctg
    13801 aaggcaaaat ttccgcatgc aaatcacatg gtttcacacc atcattcaag acttcacgaa
    13861 gttcacgttg tgtctcaaga attgctccca taccagcacc tgaaacggct tgataagttg
    13921 aaacaatgat acggtccaag ccccattttt ggcgaaccgg ctcaagagcc accatcattt
    13981 ggattgttga acaattaggg caggcaatga ttccgttgtg ggcatcaagt gcatgagcat
    14041 tgacctctgg aacaaccaaa ggaacatctg gattttgacg gaaataagat gtattatcca
    14101 ctactaccac gccagctttt actgcgtatg gtgcatactt agctgatgta gaactacctg
    14161 ctgaaaagag agcaatatca actccttcaa aagctgtttc agtcgtttct tcaattgtaa
    14221 tatcttgatc tttaaatttc aatgacttac ctgctgaacg tgcagaagca aggtaacgga
    14281 ttttatcgat tggaagtgtt gattcttcca acatttttat catctgagca ccgacagcac
    14341 ctgtcgcgcc gactacagca actgtatatc ccataaataa cctctttcgg aattttctaa
    14401 aaatttctat aatagaagta ttatactact ttttccatga attgcaaagc tttttcatca
    14461 ttttttggaa aataaaaatc ccagtcgcta gactagggac taagaggcat cttcatgtga
    14521 tgagcaggtt cacacaactc atcaaggtcc gctcctgcgt tatgacctcc ttatgctcaa
    14581 tagtagtccg aagactacct atcgactcat cgcatattct atattactag agatacttga
    14641 ttttgtcaat aagaaatttt ttcgatcgag tataacaaaa aactctcgta ttcaagagtt
    14701 tccctataca tatcatgcta attgccggga tcgaaccggc gacctcatcc ttaccatgga
    14761 tgcgctctgc caactgagct aaatcagctt acttaagaag tatactatat tcagaaaaac
    14821 ttgtcaagtt tccttagaaa ttttccatag gaaaaagcca ggtaagaact acctagctta
    14881 tcttcttcta tttacttaaa tcaattcgac gaccaatttt accaataata atcgccccaa
    14941 taatcaatga agcaaattca ccgattcctg ttgtaaacca agtaaggaag aatggtaatt
    15001 ctgctacaat atttaactct gcagcgatag taaacatgga aattgaaaag aggattgaaa
    15061 agaagaaatg atcttttcga atcaatccgt taaagaggta atccttgcta tattttgcaa
    15121 aaagccaaac acctaaactg aggaatacta aggttgaacc accaccaaca aagacatcta
    15181 gcagtccgaa gctaaagaaa ttagcaatca tacaaccgat ggtaactccg atgatgtact
    15241 taggattgta aaaagccata aagttcatca tttctgaaat acgaaactga taagcaccat
    15301 agctgatggc atttaggggc ggtgtgacag tcaaaacgac gtagatagca gcgacgattg
    15361 caatatctgc aatatcacga atagttaatt ttttcatctt ttctcctttg gcggttgccc
    15421 gcgtgtaata tgcttggtga aagaagctaa gcaccaaggg ttgctttatt caaccctact
    15481 agtatagcac aatagcctgc tttatgctat acttaacaca tgaaaattcc tattcaaaaa
    15541 ttttttaaca atgaaatctt agcctatctc ttttttggcc ttgcgacaac tctagtttcg
    15601 attctctcac gactagtcat ttatcaacta agtcatcagg aactcctggc tactgcccta
    15661 gcaaatatta tcggtatcct atttgccttt atcacaaatg atacgattgt atttaagcaa
    15721 aaaaagaaaa atagacttat ccgtttagtg aaattttctc ttgctcgcct ttctactttt
    15781 ctgttagact tgtttttaac ttttctcttt gtaacccaat ttcctcacat tatagggcaa
    15841 ttcgtaactg aaaacattga taagataaat gctattgaaa caataattgc acaaatattc
    15901 attattatca tcaattatat attgagcaag gcctatattt ttagaaaata atgctccatt
    15961 tatgagcctt ttaattgcct tctataatga tttaggatac aatgaaacac gaaacattga
    16021 aaggaaaaca gtatgttaaa aaatctaaaa tcgttcttgc ttcgaggaaa tgttattgac
    16081 cttgctgtcg gtgttgtaat tgcctctgct tttggtgcta tcgttacttc acttgtaaac
    16141 gacattatca ctcctcttat tttaaatccc gctttgaaag ctgctaaagt tgaacgtatc
    16201 gctcaacttt cttggcatgg agtcggctat ggtaacttct taagtgctat tatcaatttt
    16261 atctttgtgg gtaccgccct cttctttatt atcaagggca ttgaaaaagc acagaaactg
    16321 actggcataa agaaagaaaa aactgccgaa aaaaaaccaa ccgaattgga agtccttcaa
    16381 gaaataaaag ctctccttga gaaaaaataa tcgattaaaa ggatctagcg caaagctaaa
    16441 tccttttttc tttactcttt gggtaacttg cctgcttttt caagcatttt tttgatcaga
    16501 taaggcatct taacattctc ccgccctttt ttctcaatca gttttttcac aaattctggc
    16561 atttgtaaat cttcagattc agctaaaatg tttttcgtct cgtctgaagg aactaactca
    16621 tcaaaggtct cttcttctgg gagaaattcc ttaaactctt gattttcatt gactctaact
    16681 gtattgacca aagcatcaat caaacgagaa tctgtatttg gcattggtgg acgatggtag
    16741 tttaccccaa attcctggca taagtcataa cattctacat cattgtcaaa caagacctca
    16801 atatgctcac tgatgaaact aataggtaca aaaatataat ggtctggatg ttctgtctgt
    16861 tctctgagat actccaaaac atctggttta atccatggaa taccaatatc actctcactc
    16921 tgccaagtgt tggtatattg ttctgaactc aagcccagtt tttcagcgac tagcttgcta
    16981 ttttcgaaaa tttggtctat ataaggatca ccaaagtcca aggcaaaaat gggcacacta
    17041 tgggctgaaa agatgacttt aaagctgtct tgctttactt cttcttttaa aatcttagca
    17101 atttcatctg cccaatagtt taagagcact tcttcctgat accagtcctt aatgaccaaa
    17161 aattgaattt gtttgctttc taaaaatttt tcatagccca tgacagagta gaaagaataa
    17221 tggggctcca aaatcaagca gatacattct tcaacaccat cagtttccat ctgcttgatc
    17281 acgtctggaa taaagggacg agaaaatttg ttggcaaagt agataccgta ctcctcccct
    17341 aaacgttctt tgactaaatt gacctcctca cgtgtaatcc gctgcaaagg cgtcccaccg
    17401 atacgaatat agttgtcata gagagtctgg atttcgtggt cttggggtct aactccacga
    17461 cgaatgtttg taaagaattc tgctactcct tcaaaactaa tctcctctgg agatccaaag
    17521 gtcatcatta atatggcttt tttcatgtta tcatccttat gatattattt tctctatcca
    17581 ttctatcatg aaagaaaaaa atagtaaacg tttcctacgt ctactatttc ttttgctctt
    17641 ttgttttttt atctcgtctt ataattagga tgataaggac aagaagtaag aggacgataa
    17701 taaggagtag aaaggctaga atgtaaatcc aatgactggg aattccgtga tcgaaatacc
    17761 gtgttcctac tgcttcccca ctttcatcct gtgctacttt caccggtgca tccgttattt
    17821 tagctaaaag gatatgtcgt ccattagtta tgttaaggaa acatctcgct tgtacttggc
    17881 tttacttagc agatattgat agtaagcctg gtgttcctca tccttggaac tcaaggttcg
    17941 ataaatctcc gtgtcatagg catccacttc taaaatccca aaaatttcaa gaccacgttc
    18001 tcgaccattg tagtagatag aaccgtagcg atgctggtcg aagaattcct tatcagcaaa
    18061 tttcttaatc tcaccgaaca tagcccctga agccatagag tgtccgtaga tgatcgtatt
    18121 aaaatcagta aagttgggat taaagcgata gtcgagaaaa attgatccca ttatcgtata
    18181 atcgccaaag gcattgcggt caaggtattt gacattatcc tgactttgta gaagcggata
    18241 gtctaccttg gtcccatcaa ggctaatcca accgcaaaca tctttgttca agtgctgtag
    18301 ggtttcaaaa cctgtgctcg aagcatcctt ttcaatagga ttatagtgat catagtcact
    18361 tgcttctact actctctgcc aactaaaaag agctacaaaa acacagaaga ataccagaaa
    18421 tacgttttta aatcgcgctc ttttttgaac cgtcatcagc actcctttct atgcgagatt
    18481 tcttaaaagg aaaggaggcg ggacaaaacc gcctcttttc tattcaccct tagtcagtaa
    18541 aagggtctgc ttcttgcatt ttttttactc tctcttctac gacgtaggat caagtagagg
    18601 acaaggagta agaaaatcaa gagcaagaac aatacaaaca agataaggta ccaaatccat
    18661 agtgggattg atgagaggaa acgtccaaga gacgagtcat caaagacact gtatggaaat
    18721 ggttttgatt tttttgtatg gaaagtattt ttagggacgg tgtctgtaat ctttgcgact
    18781 acgatatgac gaccattggt cacatcgaga aaacaggtac tgagtaggat gatacgatca
    18841 ctcggtgaga gtgagatatc ccgcttgtgc atggcgactg agagcaggtg gtctagatag
    18901 gcctgctggc ggtcctcacc ctgtattcct ggatcataga tgttaaagtc ataggcatca
    18961 acctccaaca tctcaaagat ctcgagccct ttttccacac cattgtagta tatactaccg
    19021 taacgatgct ggtcaaaaaa ttcctgatca gcaaacttag ccacatcacc aaacatgacc
    19081 ccattttcta cgtggtgccc gtagataatg gtattaaagt cttcgaactt aggattattt
    19141 cgtgcatcga gaaagatagc gcctgtcgct gcaaactcac ctttggagtc cttgttgaga
    19201 tacttttcat tgtccttggc ttggactaag ggatagtcga tattggtgcc atagacattg
    19261 atccaaccga ggacttcggg attgagttct tggagtttgc tgaagcctga aaaactggcc
    19321 agctcatcct gttgcgtcgt gacaggtcga taagcttcgt actcacttga cgaagctaag
    19381 gagtagactt ggttggaatc ccaaagggcg tagaaaccaa agagaaagat caaagctgca
    19441 aaaatgagca gcaagtgatt gatcacccca tccacagcaa gaacgagacg cctagacaga
    19501 ctcttttttg gtttctcacg cttggttttc atcatctgcc cccagccgtt taggcttctc
    19561 tacgtttttt agacaagaca acaaatccag ccaaaccaag accaatcatc aaaatgaatg
    19621 gaaggttatc aatcaagaga ccagtaggag taacgtcgtc gattttgtta gtaactttgt
    19681 tgtcgttttt cttttcacct atgaggatat ctgttaatgt aaaatctaca tttttttgtc
    19741 cttgagatgt ttgttctgca ccatttgtag tataaattga tgaatctgta taacctgcta
    19801 ctccagtttc agttactgta tatttagaac ctgctggtag ttgttcaact gtaaatgttt
    19861 caccatcttt aagagtaaaa ttgtactcaa ctgtatctgc tggggtcact actgccaaca
    19921 cctctgactt agatccatgt tttactataa tattcgtaac aggggtttct gctgttttat
    19981 ttgtacttgg aagttgaatc ttgatatgga attggaaatc tcttgtttta tcccctgaag
    20041 ctccatcaac ttttttagta attgttaatg ctttcgcatt ggggtctact tgactcggat
    20101 catcaggatt aggctcctta cttgcgtctt tggtatatgt gttatcaaat aaattataaa
    20161 cgccttgttc acttggttct actttagggg cgttagattt attattttcc ttaaagtata
    20221 ctgatgaaat atagacacca ccatttactt tattcttaac aataacgtgc atttcatatg
    20281 tacgtttgtc gtatctcatg gcatcaattg ttttaccatt tttggtaatc gcctgccatc
    20341 cagtttttgc ttccgctaca tcataaacgt attcaccagc atggccataa gcaactcccg
    20401 aaaaaatgtc ttctgttgct ttttgaatct tatcagcagt tgggtgatca tttttattgt
    20461 attctacatt tctatctgga attgcaactc cggtttgata tggcgcaccg ttagaagatt
    20521 gtcctgtttt ttctgtaaac ttaaatgtaa atgtcgctgt tggtgttgtt attccctctg
    20581 caatattcaa cgttttatta atcttcacaa cagcattatt ttcaccattt gctttggtta
    20641 ttgctgaatt atcatccgca aaagtcggga cacctctgaa tacggacaaa gcggttatag
    20701 ctgcaatgct tgcagtgaat agctttttaa agaatgtttt tttcatgatt atttcttttg
    20761 tttttttatt ttcttttttc attatgatca tcaaaagcct cgtcttctaa tgactgttac
    20821 aactttgcct tgagtatcct gtatagggat ggttccaaaa gcacgactgt ctacagccgt
    20881 tgttcgattg tccccgagga caaaaagttg tcctgctggg actttcatcg ggaaggttgc
    20941 cccttcctta tagagcagcg tttctttgta gatatcttgc tcttgttgag gagaaccgtt
    21001 gatgatgaga ccattttcgt ttatatcgat tgtacttcct tcggttgcta tgacacgcgc
    21061 cactctttcc ttacctttat aactatagac tagcaaatca ccaatcgaat agcgtttatc
    21121 caacctataa tagacgacca agtcgccatc ttttaaggcg ggcttcatgc catcgtcatt
    21181 gtatcttaat aatccaaaga cgaaaaagta aaggatggct agtacaaatc caaccatcag
    21241 aagtttactg actaagtaaa ggatatctcc ccagacactc ggcggagact tatcccgatt
    21301 gacttgtgtt actggtttct tatgtttctt tttaagcagc atcacttcct cctattcacc
    21361 acaaggtgac caagtaagat gataaagaag atccctccta gaggaagaac gactccaagc
    21421 acgagagtgc tactcaagaa gtcaattccc gtttcaggta cgctgttttt attattcgtg
    21481 actgtcgcag acttatttgc atcaagagtt ccttcttttt tatcatagga cacctgatac
    21541 ccacgagacg aacttgcttc ttcttcgata ctataacgag catttagagg aaggtcgaga
    21601 atcttgatgg ttttatcttt ctttagatca actgtcgcct taccattggt gaattgtagc
    21661 gttgtttttt gattatttac tattgcacta tacgatccat tcaatggttt attttgcgca
    21721 tcctttacgt taatggtaat cttaaaggat tgcagaagat tagcaaaagc acctgtaact
    21781 ttttttgtga ctgaaagtgt cggaagtctg tggttggtga cgattgtttg aacatcctct
    21841 gacaaagtcc cctcttgttt ttcataggtg acatgatatc ccgttctaga gttctctact
    21901 tcttccacct tatagtgggt atcacgaggc agataactga gggcagtttc ctgactattt
    21961 ttgagagaaa tctgtgcttt cccgttagtc actgttgtat tcccaaactt gccgttaatc
    22021 ggcttgccat ccttatctga aaaggtaaca ttaattgtga aagatttatt aggtgattgt
    22081 tcaccttcta ggattttctt tacggtgaga cttggaagct gtcggtaaat aactttgaag
    22141 gatggtttct ctttcgtacc tgtcttttga acttcgactg tgtatcccgg agcgtctggt
    22201 gctttcagtt cgtagtcata tcccttagct acctttgcaa aactagtatt ttcagtaaaa
    22261 ctattcgttt gaactggact agtgaccttt cgataggaat cctttatact tccatctttc
    22321 ttttggttta agtttaattc gacactaggt ggacgatttg ctgatggatt tgattttcca
    22381 tccacacctt tccactcaat ctctacagga accgtcaatg gatctgacgg cttgaaagtg
    22441 ggcttctcag aatagtcttt tattttggta cgtgaggtcc caataccata ggaataggca
    22501 acttttgccc ccttgttgga gtagagttta gtagcatcgg cttcttcggc atcatcacta
    22561 gtaagtgtct tatcacccgc aatcgcatca aatgcctctt ggctagaggt gacagtaaac
    22621 ttgagaacgt acttggcgtc atcctccaaa gtgtaactcg gattaaactt ggctgttact
    22681 tccactaatc cctgttcgtt cttcttagtt tcaatcgtaa cttgattgtc attcagttct
    22741 tgctcgttac catccttgat tttgacgaca cgaaggtgtg aagcatcacc aggtaacagc
    22801 tgcacgtact tggacaagac atctgatata gttacatggt gtacgcctag aggtagaatt
    22861 ttatctgtga tttctttgaa tttttgttgc aagtcatttt cattattggc agaaaatatt
    22921 tcgttgggga ttccagaatt ataaccactg atatatcctt taagagatgt gatactatcg
    22981 tttgaaccta tataatatct gaatttgatt gaataaaagc catctatttt tggagctatt
    23041 aaactagata tattcagcat tcctctttct aaaccagagg taaaccaata tggagtatca
    23101 atcatacgtc ccacatttgg attagaataa tagttatata ttgttctccc actattagac
    23161 tcgtaataca tattagcttc gccatctgac agcagaataa ctactttttt tgccccagcc
    23221 cttgcacttt gcaacaatct ttgagcttca gttaacccag catttatatt tgtcccagtg
    23281 ccaatgcccg cgtccatacc ataagaacga ctagagtcag caattgttaa cgaactaaca
    23341 atattttttg agctattaga atccgtgctc caagatacta aagttttagc atcatcatat
    23401 gaagagatat ttgaatacca accagctcaa tttggccaag ttccatattg ataactcaga
    23461 taatattcat tataataacg gtcattatac tgattatcaa tctttccggc aaaaccaact
    23521 atagacagac ggttttggga attagaaaga attgtatcaa tgagcccctg acgaccattc
    23581 acacctttca gtgtatgttt tagtgcttca atcctactaa tgtatcgtct actagagtaa
    23641 gaccacacat ctcgctcttc catactccct gaaagatcgg caaccaagac tacatccaag
    23701 ggttctgact gggtctccgt tcccagttta gatgtgatat ccagtgacaa ctcatactgg
    23761 tctttttgcc ctgatatcgg agtaatcgtt ttgtgcagag ttgtctgagg ttcagttatt
    23821 gaatctgcac gtaaagaatt aattggtgca cacaaaaaaa gaatagcgag aaagataact
    23881 aaactactgg taaaatattg aatcttctta aaatcatatt gaacgttcat gctatctccc
    23941 ttgctagaat atgttttaat ctgatactat ataaatctga gacttccata agataattgt
    24001 ataataatat taacttcgtg tcaattaatg tgtttgaaaa catgttatta taaattacaa
    24061 aaaagtatga ttcttctttg tttttcaact ttttctgtgg taaaatggaa aaaatagtaa
    24121 aaaatttcat aaaataaaga tgcatatatt tattctaatc gctcgaaaat ttcttttgaa
    24181 agtcttctga atttgaatta ttatattttt tcaaaattca taaaatttct caaatcatat
    24241 ttttccgaag ttttgtattc tattttatga taaaaaagga gtttttttat gacttatcca
    24301 aatctcttgg accgcttctt aacctacgtt aaggtcaaca cgcgctctga tgaacactct
    24361 actactactc caagtacaca gagtcaggtt gacttcgcaa caaatgtcct aattcctgaa
    24421 atgaaacgtg ttggactgca aaatgtttac tatctaccga atggttttgc tattggaacc
    24481 ttgccagcca acgatccgtc tttaacacgt aagattggtt ttatatcgca catggatact
    24541 gctgatttta atgctgaagg agtcaatcca caggtaattg aaaactacga tggtggtgtg
    24601 attgaactag ggaattctgg tttcaaactc gatccagccg acttcaagag tcttgaaaaa
    24661 tatccaagac aaacactcat cacaacagat ggaacaacct tgctaggtgc tgatgacaag
    24721 tcaggaattg ctgaaattat gacagccatt gaatatctaa ctgctcatcc tgaaattaag
    24781 cactgtgaga ttcgtgttgg ttttggtcca gatgaagaaa tcggtgttgg tgccaataaa
    24841 tttgatgcag aagattttga tgtggatttt gcctacactg ttgatggtgg tccactaggt
    24901 gaacttcagt acgagacttt ctcagccgct ggtgctgaat tgcatttcca aggccgtaat
    24961 gtccaccctg gtactgccaa agggcagatg gtcaatgccc ttcagctagc aattgatttt
    25021 cataatcaac ttccagaaaa tgaccgacct gagttaactg aaggttacca aggtttttac
    25081 catctaatgg atgtgacagg tagtgttgag gaggtgcgtg caagctacat cattcgtgat
    25141 tttgaaaaag atgcctttga agcgcgtaaa gcatccatgc aatctatcgc tgataagatg
    25201 aatgaagaac ttgggagcga ccgtgtcact ctcaacttga cagaccagta ctacaatatg
    25261 aaagaagtca ttgaaaaaga tatgactcca attaccattg ctaaagccgt tatggaagat
    25321 ctaggtatca cgcctattat cgaaccaatc cggggtggaa cagacggctc taagatttcc
    25381 tttatgggaa tcccaactcc gaatatcttt gcaggtggcg aaaatatgca cggacgtttt
    25441 gaatacgtta gccttcagac tatggaacgt gcagttgata ccatcattgg catcgtagct
    25501 tataaaggct aaaaagacga ggtggctcag ctacttcgcc tttcttttta ttctactggt
    25561 ttttcttgat ttccagtagt tgtagaagat tctgttgttt cattttctga agttgattca
    25621 gcaggtttag aatctcttgt attgcttggt ttgttttcgt cgctagcagt ttcaatgtta
    25681 gattctgcag ttgcgtttgg ttggttctca gcactggtgt tatcactatt tgcttcagca
    25741 tttcttgctg gacttgtttc ttcacttgcg ctaacttttg actggatttg atgattcaaa
    25801 actagaatag cttttgtcga ttcaagtaaa gctgttttgt ctttactatt agcagaaagt
    25861 tgatctaata atgcatccac cttatcaaag tccgcatcag atccattatt actttctaaa
    25921 taagagtgaa gcgacatgag aatatcgtag agtttttgat agagtacaag tgtctgagaa
    25981 tcttgctcag cattttcctt ttcttgttga agggcgctag cgatacgagt caaaacatct
    26041 tttacctgac tgtttacttc atccaagtct gcatcagcct tgtttgtggc agcttttaga
    26101 ttttctactt cttctgccaa ggattgtctg attccttctt catggatttg ttccaagagt
    26161 tgatttgcct tgttcaaaag actttctact tcttccttgc tatctgtcgc agattattgg
    26221 ttgctatcta ccatgtactc ctaaaacagg agagttataa tccaagatta caaggcctta
    26281 cagaaataag aaatccagat aagacaatgt tcgtccaaga cgctattcgc ttcgcacagc
    26341 agcacggatt caatatgctt taattttaaa gtttaggtgt caagacctct ttttagtgtg
    26401 cccaaaattt agagaagtaa tcaatcaact aacttttatt tttttcaaac tttcagtaaa
    26461 ctgacctaaa gctaactcaa tctgtctttg tagatgcttc tgctatcagc tagaagttga
    26521 tctacttttg ccaagactgc cttctcatca aaagttccag gttgatagtt ggattgcagg
    26581 gatggaatct tgtttttcaa agccgcttca tatcccttag tttgaacctt gatgtagtga
    26641 ttgtggtcgc catgaggaat cacaaaacct tctgaatctt cacttataat tcgattggca
    26701 tcaaaaacca taaccatctt cttcctcatg gtggacatgt agtgacggat tacttaatac
    26761 agaactagaa gaacttccta cctcttccgt gttagagtgt gatgggggat tgttaagaga
    26821 tgacttagga atatagtgat agtgttcccc atgtcttact atataagcat cacctatatc
    26881 tctgacaata tcattagggt taaagacata tgtggctgct aattcacctg ccgacaagtc
    26941 actctcagga atgaaatgat agtgaccacc atgtggtact attagtagat tgaaatagaa
    27001 tatgagcaaa ttgataaggg gattttaaag taatttctaa caatgattta gaaactatga
    27061 tgtgctattc taaattcaac tcactatata taaccatcat cggtagtata acgtccctgt
    27121 aattttgcta cagatacttc tgcactagct cctttatcgt ctttaccatg ttcttgtttt
    27181 tggcgattga tttcatcttt tgttcgtaca ttttctgcat gagcttgatc tttaaggtaa
    27241 acataatact ttccatctac cttaataata tatcctccct taacctaact gacaatatct
    27301 tgatctttcg gctgatagtt gggggctttc attaatggct cttcactaaa gagcgcatca
    27361 aaaggaactt taccattata gtagtgataa tgatcgccat gagaagttac ataaccttga
    27421 tctgtaatct taataacaat ttgttttgct tgaattcctt ctttttgact aacctagtct
    27481 ggagtcaaat tttcagtctt cttagtgtct ttattactgt ttacatatga aacacgattt
    27541 ttatctgtat tggcctgtta gctatgttgg ttcagagcat aaacacacag acttaaggaa
    27601 aggataacaa cagatccagc tgctatatat ttctttttaa atttcataat tacctcattt
    27661 ctataattat ttatatgatg tcttcattat taaatgatta tagaaattaa ttaaccaatt
    27721 aattaactag taaatattcc acctcttttt aagttgtatg tcaagaaatt ttatatatta
    27781 ataataaaat gaaattctcc caaagtcaga gttttatttc taacttttga gagaacttca
    27841 tttttgattc agactttttc tactgctatt ccttacgcta tgagatcaga taaattcttt
    27901 tttatcactt ctccacttgg caatcttaat tcaatcgttc catccatatt gaatataaca
    27961 ctatctaagc ctaatccgta actagctgta aatttttcta atttttcttg tacaggatct
    28021 actgctggag cttcctctaa tgctggatct aacatagggt cactccccac attcccttct
    28081 ggattcaaca ttccattatc cgttgagttt tctggtttta caggtttttc gtttggtgcc
    28141 tctggtaaag aatctgctgg tttattttct gttggttggt tctcaactgt tccagtagat
    28201 acttttccat tttcagatgg tttattttca ccatttcctt gaggtgcttc tcctgtaaaa
    28261 tctgccatat tctttttaat gacttctccc gatggtaaat ataattcaat tgttccgtcc
    28321 atattaaaca agacattttc tagcttcatc ccataacttt cagcaaattt tgctactttt
    28381 tcttgtacag gatccactgt aggaacttct tctaacgttg aattactagt actattccca
    28441 gtttcagaaa gtttttcttt ttctaccttc tcactagtct ttggttcttc taccttttca
    28501 tcaagttttg agttttcttg tgctttattc cttttaaatt gtggtagaat acttggttta
    28561 tcagtttgat tttctttttc caagataggt acttccacaa tataagtcga ttgattgtcc
    28621 aaataagcat ttgccatgaa ggttacagga attttatttc cggccgttct ggttgttcct
    28681 tggtttaatt tcggaatcgg taatttgatt tcaccaactt tatagttatt ttctaaataa
    28741 gcatttccat gaaattcatc aaacactctg actaaagcat cagttccttt aggcactgca
    28801 aattgagggt tcactcttaa ataagtatcc cctgcatgga aaggatagaa aatcgtttga
    28861 ctggccattt tgtaagctaa agaggttgga actgtaaatg taccatcata acttacttct
    28921 ggataatctt ttgaagcgat agtatactta aatgtttgtc ctggtaaata aggttgatct
    28981 aattcaaagt ttgcaatatt ccctactcct tctccaaata ctttaccaga tactttctcc
    29041 aatacttttc catctggtgt tattaatttt actagcatat tgatacctaa ttttttctcc
    29101 aattcaggcg gaaaactaaa agaaacgcgt ttttgaccat tggctagagt aaagttttga
    29161 ttattaaacg tactattttt taacaaatta acaacattcg ttaattcttc tccagtataa
    29221 actttattcc cttctttttt agcaactcct tcttcgggtt taaacagttc atagttactg
    29281 tgagaatgac caattccaac cggtttatgt tcatcaatcg gatctgcatg atggtgatct
    29341 ccatgcggat aaataatcgc atttttttct ttattcacga caatactttc acgtttgaca
    29401 ccatattgtt tcataatgcc agcaattttt tcttcgattt ttttatctaa atctttcatt
    29461 tctttggcat tacttggata atcctgttca tgagatgaca aagaatctaa tccattatga
    29521 ctagttttaa cttcctctaa atgtttttgc gcagccttaa tttgctcttc tgtcaagtcc
    29581 ttcttgaaga aataatgatt gtggtctccg tgactcatga caaaacctga ttcatcttca
    29641 gcgataatac gattagcatc aaatccgtat ccatcttctt catgtttctc atgtgaagtt
    29701 cctggattga ttggaagaga tggagaaggt gttgctagac tattgtttgg aagagtcggt
    29761 tgcccaattt gatttgattt tggaatgtaa tggaaatgat caccatgtct tacaatataa
    29821 gctgtagccg tttcttcaac gatatctttt ggattaaaaa tataaccatc agatgctgaa
    29881 gagagctcct tacttgtcgt taaagaagaa ggattgcttg aaagactgcc tagactagac
    29941 actacttcat taggttttgc atttgtagaa actgtagaac cagttccact gataggcacc
    30001 attctggcaa tcttttcttc taaggcagaa agcttgctgt aaggaataaa gtggtaatgg
    30061 tcgccatgcg gaatcgcaac tccatttggt gtacgactga taatcttagc agggtcaaag
    30121 accaggccat ctgattcact gtaacgttgg gcgctaggtg aatcatagag ttccttcaaa
    30181 agactctgga gattttcaga tttatttgct ggcttgctag ttgatccttt tgctacagat
    30241 tgcgtgttat tgtcactagc tgttgaagaa tagcttaact gactcggttg catatttttt
    30301 ccagccagat gtgctttagc tgctgctaat tcactagcag ataaatcgct tttgggaatg
    30361 tagtgatagt gacctccatg aggaacgata taagcattac ccgtatcttc gataatatca
    30421 gctggattaa agacataacc atcatttgtc gtatatcgtc cctgagacct tgctacagca
    30481 acattagagt taaccttctc attatctttg acatgttctt gtttttgacg attgatttca
    30541 tctttagttc gaacattatc agcatgagct gcatctttca ggtagacata atattttcca
    30601 tcgaccttga tgatataacc acccttgact tcattgacaa tatcagcgtc tttaagttga
    30661 tagtttggat ccttcatcaa gagttcttca ctaaagaggg catcataagg aactttccca
    30721 ttatagtaat gatagtggtc accgtgtgac gttacatagc cctgatctgt aattttgatt
    30781 acaatttgct cggcctgaat tccttctttc tggctaatct ggtctggtgt caagttttca
    30841 cttttctgac ttgactggct gccatctaca taagagacac gattattgtc cttattttcc
    30901 tgcgaacgat gctggtttag tgcataggca catagactca aggatacgat aacagctgat
    30961 ccagctgcta tatatttttt actgaatttc ataaatccct ca
//
LOCUS       DAVBHS010000007        29809 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVBHS010000007 DAVBHS010000000
VERSION     DAVBHS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29809
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.007"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(168..581)
                     /gene="ndk"
                     /locus_tag="VQ651_000215"
     CDS             complement(168..581)
                     /gene="ndk"
                     /locus_tag="VQ651_000215"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET2753265.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(692..4369)
                     /gene="rpoC"
                     /locus_tag="VQ651_000216"
     CDS             complement(692..4369)
                     /gene="rpoC"
                     /locus_tag="VQ651_000216"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET2753266.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGEVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPYAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(4402..8013)
                     /gene="rpoB"
                     /locus_tag="VQ651_000217"
     CDS             complement(4402..8013)
                     /gene="rpoB"
                     /locus_tag="VQ651_000217"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET2753267.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSSLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            8429..8499
                     /locus_tag="VQ651_000218"
     tRNA            8429..8499
                     /locus_tag="VQ651_000218"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8461..8463,aa:Cys,seq:gca)
     gene            complement(8697..10028)
                     /locus_tag="VQ651_000219"
     CDS             complement(8697..10028)
                     /locus_tag="VQ651_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HET2753268.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(10319..11143)
                     /gene="endA"
                     /locus_tag="VQ651_000220"
     CDS             complement(10319..11143)
                     /gene="endA"
                     /locus_tag="VQ651_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HET2753269.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQ
                     APEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(11182..11370)
                     /locus_tag="VQ651_000221"
     CDS             complement(11182..11370)
                     /locus_tag="VQ651_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET2753270.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDSW
                     AILSPAKWQELIHKFTGN"
     gene            complement(11363..12646)
                     /gene="murA"
                     /locus_tag="VQ651_000222"
     CDS             complement(11363..12646)
                     /gene="murA"
                     /locus_tag="VQ651_000222"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET2753271.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(12670..12900)
                     /locus_tag="VQ651_000223"
     CDS             complement(12670..12900)
                     /locus_tag="VQ651_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HET2753272.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTAENTRKVRV
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(12958..13995)
                     /locus_tag="VQ651_000224"
     CDS             complement(12958..13995)
                     /locus_tag="VQ651_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET2753273.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(13979..14467)
                     /gene="coaD"
                     /locus_tag="VQ651_000225"
     CDS             complement(13979..14467)
                     /gene="coaD"
                     /locus_tag="VQ651_000225"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET2753274.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLCVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(14457..14996)
                     /gene="rsmD"
                     /locus_tag="VQ651_000226"
     CDS             complement(14457..14996)
                     /gene="rsmD"
                     /locus_tag="VQ651_000226"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET2753275.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKTVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(15061..16053)
                     /locus_tag="VQ651_000227"
     CDS             complement(15061..16053)
                     /locus_tag="VQ651_000227"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HET2753276.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(16165..16848)
                     /locus_tag="VQ651_000228"
     CDS             complement(16165..16848)
                     /locus_tag="VQ651_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET2753277.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(16904..17644)
                     /locus_tag="VQ651_000229"
     CDS             complement(16904..17644)
                     /locus_tag="VQ651_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET2753278.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            17678..17956
                     /locus_tag="VQ651_000230"
     CDS             17678..17956
                     /locus_tag="VQ651_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET2753279.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            18034..18960
                     /gene="yidC"
                     /locus_tag="VQ651_000231"
     CDS             18034..18960
                     /gene="yidC"
                     /locus_tag="VQ651_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET2753280.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(19035..19829)
                     /gene="pflA"
                     /locus_tag="VQ651_000232"
     CDS             complement(19035..19829)
                     /gene="pflA"
                     /locus_tag="VQ651_000232"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HET2753281.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(19955..21205)
                     /locus_tag="VQ651_000233"
     CDS             complement(19955..21205)
                     /locus_tag="VQ651_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET2753282.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(21272..22099)
                     /gene="purR"
                     /locus_tag="VQ651_000234"
     CDS             complement(21272..22099)
                     /gene="purR"
                     /locus_tag="VQ651_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HET2753283.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLHEFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(22366..23307)
                     /locus_tag="VQ651_000235"
     CDS             complement(22366..23307)
                     /locus_tag="VQ651_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HET2753284.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(23297..24553)
                     /locus_tag="VQ651_000236"
     CDS             complement(23297..24553)
                     /locus_tag="VQ651_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET2753285.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(24555..25217)
                     /locus_tag="VQ651_000237"
     CDS             complement(24555..25217)
                     /locus_tag="VQ651_000237"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HET2753286.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDGFVGVDRGSLWILE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFKQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLASYMHQIEIEDGQNLITYCPEGISQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(25180..25836)
                     /locus_tag="VQ651_000238"
     CDS             complement(25180..25836)
                     /locus_tag="VQ651_000238"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET2753287.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDGHFVP
                     QISFGAGVVEALRPHSKMVFDCHLMVSNPEHHLEDFVRAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALRDEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(25847..26725)
                     /gene="rsgA"
                     /locus_tag="VQ651_000239"
     CDS             complement(25847..26725)
                     /gene="rsgA"
                     /locus_tag="VQ651_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET2753288.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDGGELDFYQQTYGDIGYDFVTSKEELLPLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     TAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGTIAPFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(27318..28091)
                     /locus_tag="VQ651_000240"
     CDS             complement(27318..28091)
                     /locus_tag="VQ651_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HET2753289.1"
                     /translation="MRKKLVNSLMCAVVLSGFPIATYANEKNIGPNITVSNGQTRLSI
                     PEKNGVEVIQSEENRVVYEVKEAAKIETSDGDMVAPIVVDSRITSINNQVVQGKTVYT
                     ADLEKATFVEEGYNKLAKPTNILDHIFGIQKVHAYTQNITTHQSQFDSTYSVELYTVV
                     HWVSYDGGVQKNITKISGGVDFHERDIEIESSSVEVRQGGWYSSQQKWFHPGARSSWE
                     YSTGFSKVGPGENRVVTNYTVNLIRYGDRWSAYLQNNPW"
     gene            complement(28781..29653)
                     /gene="rsmA"
                     /locus_tag="VQ651_000241"
     CDS             complement(28781..29653)
                     /gene="rsmA"
                     /locus_tag="VQ651_000241"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET2753290.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAEVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPVPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLE
                     EFASLADALKGQGL"
BASE COUNT         8479 a         6916 c         5746 g         8668 t
ORIGIN      
        1 ttttttgtct cgcacctaac ggagcgagac gaactaatag tcacttaatc aaaaaacgca
       61 ccatatcaaa aactaaaaag tttgatatca tgcgtcatgt cttaaactaa ttgactatac
      121 tttctattca aatgagcttt taaccaattg attgagccaa tccactctta aaaccaaaga
      181 gcaatttctc gcttagctga ctcttctgaa tctgaaccat gtacaacatt ttggataacc
      241 tcattttctc cagcagcttt tgcaaaatca cctcgaatag tgcctggtaa agcttcttct
      301 ggacgagttg cacccatcat ggtccgccaa gtttcgatta ctttgggacc agaaatgaca
      361 cccacaagaa ctggacctga agtcatgaat tcacgaatcg gtgggtaaaa actctgacca
      421 accaagtcct gatagtgctg gtcaatcaac tcttctgaaa cttgtgaacg aaactccaat
      481 ttttcgattg taaatccacg ttgttcgatg cgctttaaca cttcacccac tagccctctt
      541 tttacaccat ctggtttgat gataaagaat gtttgttcca tacccgtctc ctttgtcagc
      601 ttctttcttt tattttacca catttcgtgg aaaaatggag aaagttttca gaatagagaa
      661 tgagagaacc cgagggttct ctcattctct attattctac tgtttcttcc acagtttcaa
      721 cggcagtatc cacaactact tctgttgttt cttcatttcc ttcttcctct actggaggat
      781 taaggtattc ttcttcgttg acagcatatg gttcaaggtt acggtaacgg gccataccag
      841 taccagctgg gatgatctta ccgatgataa cattttcttt aagtccaagg agatggtctt
      901 tcttaccacg gatagctgcg tcagtaagga cacgagttgt ttcctggaag gaagccgctg
      961 acaagaaact gtttgtttca agtgaggctt tggtaattcc cataaggact gggcgacctg
     1021 tcgctggaac tccacctgcg ataaggacat ctttgttggc atctgtaaag tcattgatat
     1081 ccatgagggt acccatgaga agatctgtat cacctggatc catgacacgg actttacgga
     1141 tcatttgacg aaccattacc tcgatgtgtt tgtcaccgat ttctacccct tggctacggt
     1201 aaactttttg tacttcaccg agaaggtacg tttcaactga caagacatca cgaactgcaa
     1261 ggagacgttt tggttggata gaaccttctg tcagagcagc accacgcgct acttggcccc
     1321 caacttcgac acgcatacga gctgtaaatg gaacgacata ttcaccttcg ccagtttcac
     1381 ccttaacaaa gactttcttg gtacgagttg atgcatcttc ttcgatagca gtaacttctc
     1441 ctttaacctc tgtgataacc gcttcccctt taggattgcg ggcttcaaag atttcttgga
     1501 cacgaggaag accctgagtg atatcggtat ttgaggcaac cccacctgtg tggaaggtac
     1561 gcattgtaag ctgtgtacca ggttccccga tagattgggc agcaatggta ccaactgctt
     1621 caccaacttc aaccgcatca ccagtcgcca agttgatacc gtaacagtga cggcagacac
     1681 cgtgacgagt gttacatgta aatacagaac ggatagtcac ttcttccaca ccagcattga
     1741 caatttcacg cgccttgtct tctgtaatca attcatttgg accaataatc actgcaccag
     1801 tttctggatg tttaacagtt ttcttagtgt aacgaccgtt gagacgctct tcgagagact
     1861 cgatcatctc ttttccttct gcgatagaac ggatcaagag accacggtca gttccacagt
     1921 cgtcctcacg gatgataacg tcttgggcaa cgtcgaccaa acgacgagtc aagtaacctg
     1981 agtcggctgt cttaagggcc gtatcggtca tacctttacg agcaccgtga gttgagaaga
     2041 acatttccaa taccgacaaa ccttcgcgga agtttgaaag gattggcaat tccatgatac
     2101 gtccattcgg agcagccatc agaccacgca taccggcaag ctgtgagaag tttgagatgt
     2161 taccacgggc tccagagtcc atcatcataa cgattgggtt cttaggatct tggttagcaa
     2221 tcaagcgttt ctcaagtttt tcacgggcag cacgccattc agctgtaaca gcattgtaac
     2281 gctcgtcgtc tgtgatcata ccacgacgga attgtttggt gatttgctcg acacgtttgt
     2341 gtgattcttc aatgatttca gccttgtcat caacgactgg gatatcggca atacccactg
     2401 tcaatcctgc aagagttgag tggtggtaac cgagattctt catgcggtca agtagggcag
     2461 aagtttctgt cgtacggaaa cgtttgaaga tttcagcgat gatatttccg aggtttttct
     2521 tcttgaacgg agggttgagc tcaagattgc tgatagcttc cttgatatct ccaccaagtg
     2581 gcaagaagta tttagctgga acaccttctg tcaagttggc attgtttggt tcttgcaagt
     2641 atggtagccc ctctggcatg atatcgttga agagaatttt accaactgtt gtaagcaaga
     2701 ccttatgtct ttgctcttct gtccaaggct tgttgaggct gtctgttgcg ataccaacac
     2761 gtgagtggag gtgaacataa ccattgcggt aagccataac cgcttcgtca cggtctttga
     2821 agaccattcc ttcaccttcg cgaccagctt cttccatggt caagtagtag ttacccaaaa
     2881 ccatgtcctg agatggagta actaccggtt tcccatcttt cggattcaag atgtgctcag
     2941 cagctagcat gaggatacga gcttctgctt gtgcttcttc tgaaagtggt acgtggatgg
     3001 ccatttggtc cccgtcaaag tcagcattgt aggcttcaca gacaagtggg tgcaagcgaa
     3061 gagccttacc atcaatcaag actggctcga aggcttggat acccaaacgg tgaagggtcg
     3121 gtgcgcggtt caaaagcact gggtgttctt taatcacttc ttcaaggata tcccagatac
     3181 gctcatctcc gcgttccacc aagcgtttag ctgctttgac gttttgcacg atatcacggg
     3241 caacgatttc acgcatgaca aatggtttaa agagttcaat cgccatttca cgtggcacac
     3301 cacattggta catcttaaga gttggaccaa cggcgataac ggaacgtcct gagaagtcaa
     3361 cacgtttacc gagcaagttt tgacggaagc gtccttgttt acctttaagc atgtggctca
     3421 atgatttcaa tggacggcta cctggtcctg tgattggacg accacgacga ccattgtcaa
     3481 tcaaagcgtc aactgcttct tgaagcatac gcttctcatt ttgaacgatg atacctggtg
     3541 catttaactc aagcaaacga gccaaacggt tgttacggtt gataacacgg cggtaaaggt
     3601 cattcaagtc agatgaggca aaacggccac catccaactg caacattgga cgaagatctg
     3661 gtgggataac cggaagaatg ttaagaatca tccattcagg tttgtttcca gacttgtaaa
     3721 aggcatccaa aacatccaaa cgacggatgg ctttgacacg tttttgtcca gtagctgttt
     3781 tcaattcttc tttgagttca gcaatttctt tttcaagatc tacttgcttc aaaaggtctt
     3841 ggatggcttc cgcacccatc ttggcaacaa atgaaccata accatattca cgcaagcgct
     3901 ctcggtattc gcgctctgtc atgatagact tgtgctcaag tggtgtatcc ttaggatcaa
     3961 tcaccacata agccgcaaag tagataactt cctcgagggc acgagggctc atatcaaggg
     4021 tcaagcccat acggcttgga atccccttga agtaccagat gtgagataca ggagctttca
     4081 attcgatatg tcccatacgc tcacgacgaa ctttcgtacg cgttacttca accccacagc
     4141 ggtcacaaac aattcctctg taacgaatgc gtttgtactt accacaagca cattcccagt
     4201 cttttgtagg accaaagatc acttcatcaa agagtccttc acgttctggt ttcaaggtac
     4261 gataattgat tgtttcaggt tttttgactt ctccataaga ccatgaacgg actttacttg
     4321 gagaagctag ggtgatttgc atacttttaa aacgatttac atcaaccact atttcttccc
     4381 tttctattct aagtgaactg cttattcttg ttcagcagct tcttctgttg cttccgcttt
     4441 tgttgctttc tcagcttctt cagcttcaaa ggctgcttta gcctcttgag ctgctttttc
     4501 gcgggctttt tcaaggtcat ctacgtggat gacatcttcg tccattcctt catccaagtc
     4561 gcgaagttcc acttcttggt catcttcgtc taggacacgc atgtcaagac caagagattg
     4621 caattctttg acaagaactc ggaaggattc tggaacacct ggttttggaa ttggtttgcc
     4681 ttttgtaata gcttcatagg ctttcaaacg tccgttgata tcgtccgact tgtaagtcaa
     4741 gatttcttga aggacatttg acgcaccgta ggcttcaaga gcccaaacct ccatctcacc
     4801 gaaacgttgt ccaccaaact gagctttacc tccgagtggt tgttgggtaa cagttgagta
     4861 aggtccgact gaacgcgcat gcaatttatc gtcaaccatg tggtggagtt tgatcatgta
     4921 catgactcca acagaaacac ggttatcaaa tggttcacct gtacgtccat cgtaaaggat
     4981 tgtcttggca tcgctatcca tacctgcttc tttaacagtt gaccaaagat cttcagaact
     5041 tgctccatca aagactggtg tcgcaatgtg aataccaaga gtacgagctg ccataccaag
     5101 gtgaagctcc ataacctgac cgatattcat acgtgatggc accccaagtg ggttcaacat
     5161 gatgtcgact ggagttccgt ctggaaggta aggcatgtct tctacaggaa cgatacgaga
     5221 gacaacccct ttgtttccgt gacgtccggc cattttatct ccgaccttaa tcttacgttt
     5281 ttgagcgatg taaacacgaa ccaacatgtt aacacctgat tgcaactcat ctccatttac
     5341 acgtgtaaag atcttaacat cacgaacgac accatcggca ccgtgtggta cacgaagaga
     5401 agtatcacgc acttcacgag acttgtctcc aaagatagcg tgcaagagac gttcttcagc
     5461 tgaaagatct ttctcaccct taggtgttac tttacctaca agaatatcac cttctttaac
     5521 ctcagcacca atacggataa tccccatttc gtcaaggtct ttgagggcat cttcaccaac
     5581 gtttggaatt tcgcgagtga tttcttcagg cccaagcttt gtatcgcgcg tttctgattc
     5641 gtattcttca aggtgaacag atgtgtagac atcgtccttc accaagcgtt cgctcatgat
     5701 aacggcatcc tcgaagttgt aaccttccca agtcatgtag gcaacgattg ggttttgtcc
     5761 aagcgccatt tctccatttt ccatagaagg tccgtcagcg atgaaatcgc ctttttcaac
     5821 gacatcacca acttttacga gagtgcgttg gttgtaagca gtacctgagt ttgaacgacg
     5881 gaatttttgg atgtggtaaa catccaatga accatcttca cgacgaactt ctaccttgtc
     5941 agcatctgcg taagtaactt taccatcata ctgagcaatc acagccgcac cagaatcgtg
     6001 ggctgcttgg tattccatac cagtaccaac gtaaggtgcc tgaggattaa tcaatggcac
     6061 agcctgacgt tgcatattgg ctcccatgag ggcacggttg gagtcatcgt tttccaagaa
     6121 aggaatacat gctgtcgcaa cggcaactac ctgttttggt gaaacgtcca tgtagtcaac
     6181 aatattagct ggatactctt ggttgacccc ttggtgacgt cccatgacaa tcttctcagc
     6241 aaaggttcca tcttcattca gactagagtt agcctgagct acagtatatt catcttcttc
     6301 atcagctgtc aaccaaacaa tttcgttcgt gacaacacct gtttcacggt caaccttacg
     6361 gtatggtgtt tgaacaaaac catatttgtt caagtgtccg taagatgaca agttattgat
     6421 caaaccgatg ttaggtcctt caggtgtctc gattggacac atacgaccat agtgagtgta
     6481 gtgcacgtca cgtacttcat atccagcacg gtcacgagtc aaaccaccag gtcctaaggc
     6541 tgacaaacgg cgtttgtgag acaactcaga aagcgggttg tgttggtcca tgaactgtga
     6601 caactgtgat gaaccaaaga attctttaac tgcagctgtt acaggacgga tattgataat
     6661 ttgttgtggt gtcaagactt cattgtcctg aacagacata cgttcacgga cattacgttc
     6721 catacgagaa agtcccaaac gtacttggtt ggcaagcaat tcaccaaccg cacggatacg
     6781 acgatttcca aggtggtcga tatcatctac acggccaagt ccttcagcca agttgaggaa
     6841 gtagctcatc tcagcaagga tatctgcagg agtcaccgta cgaaccttgt catctgggtt
     6901 agcattacca atgatcgtta cgacgcgatc tggatcagtt ggagcaacaa ccttgaattt
     6961 ttgaagaaca acaggctcag tcacaacggc tgcatcgttt gggatgtaga caatcttgtt
     7021 caagtcgcca tccaaatggc tttcaatgct ttcaatcacg ctacgagtca taatcgtacc
     7081 agcttctacc aagatttctc cagtttcagg gtctaccaat ggctctgcaa tggtttggtt
     7141 gagcaaacgt gttttaacat tgagtttttt attgattttg taacgaccaa ctgctgccaa
     7201 gtcatagcga cgtgggtcaa agaagcgagc cacaagcaag ctacgtgagc tttcagctgt
     7261 cttaggctca cctggacgaa ggcgttcgta aatttctttc aaggcttcgt ctgtacgaga
     7321 gtccattgga ttcttgtgga tatctttttc aacagtgttg cgaaccaatt cgctgtcacc
     7381 aaagatatca aagatttcat catcacctga gaaaccaaga gcacgaacca aggttgtaaa
     7441 tggaatctta cgagtacggt cgatacgagt gtaggtgata tcttttgagt cgctttcaag
     7501 ttccaaccaa gctccacggt tagggataac agttgaacca tagcccacct taccattttt
     7561 gtctactttg tcgttaaagt aaacacctgg tgagcggacc aactgagaaa cgataatacg
     7621 ttcaccacca ttgatgatga aagtacccat ttctgtcatg attgggaaat caccaaagaa
     7681 aacttcttgg gtcttgattt cgcctgtttc tttattgatc aagcggaagg ttacaaaaat
     7741 tggtgctgag tagctagcat cgtggatacg agcttcttct agcgtgtatt ttggttcctt
     7801 gatttcatat ccaacaaatt ccaactccat tgtgtctgtg aagtttgaaa ttggcaatac
     7861 atcttcaaac acttccttaa gaccgtggtc taggaaagct ttgaatgagt cagtttgaat
     7921 ttcaatcaaa tttggtaagt caagaacttc tttgattctt gaaaaactac gacgggtacg
     7981 atgtttcccg tattgaacgt catgtcctgc caagatgatt ctcctttgta aataagttcc
     8041 aagccttgtc aatcaggctt ttctaatcgc catatggttg taaacccctt atcaccgtgt
     8101 cctcttgacg aattttcaga atctttaagc ctctgttaca aatgctcaaa atcttgaaaa
     8161 aaagcacaaa aagagcagct aaatctgact ttttcagaag atttaactgc tgtgagcctt
     8221 gtctggacaa tatttcagac aaaacctacg acaaatgatt acccatatta taccctattt
     8281 agctagattt ttcaagggga ttcagtaggt ttttggtaat ttttttccca tagaaaactt
     8341 ggcatcacat tcgaatcacg ctatggtaca aaaaactgaa aaaactattg actgaaaatc
     8401 attttcaagg tataataata aacgttaagg cggtatagcc aagtggtaag gcacggctct
     8461 gcaaaagctt gatcgtcggt tcaaatccgt ctaccgcctt ctataacttg atttatcagg
     8521 tttcaaatga acagaaagcc caatttgaag ggcttttttt attttccctc gaataaatac
     8581 gtataacttt aaaaactttt ggagcgagtt tgtggcagag ttctttccat ggcataattc
     8641 ccttttgaaa tcaattgaaa aaagcttcgt cacgcctggt gacaaaagcc tctagtttat
     8701 tctgtttctt cttctatttc gacttctgtg atttggactt ttaccttggt aacacgtcca
     8761 ttcttcactt tatcattggt taagataaac tgtttgtttt ggctgaccaa ttcatagctg
     8821 agtttctcag tcgttggaat ggttcccact cctgtcaaat aataaccagc gatagtatca
     8881 acgtcatcac tttccagttc aacattaaag taattattga aatcattgag ggtcatagtt
     8941 ccttgaacaa tgtaggtatc ctcgccaatc tgatgaacat ctattgctgc tttgtccgtt
     9001 tcatcatcaa tttcaccaac aatttcctct aagaggtctt ccaaagtcac caaaccagcc
     9061 ataccaccgt attcatccag caagattgcc atttgtcttt gggtatttcg cagttctttt
     9121 agcaagtcat ccacaaaaat agtttcaggt acaaaaagtg gatcttgtaa aattctcttc
     9181 caaacaatat tgtcaaaacc gtccacaaag cctgccttaa ggagactctt ggtgtgaatg
     9241 attccaatta cattgtcctt atccccatca taaaccggga tacgagaata attttgtttt
     9301 aaaatacttt ggataatggc ttgactatca tcctgaatat ccaccataaa ggcatccgtt
     9361 cgaggaacca taacctctcg tgccatcagt tcatcgagcg aaaagacacc ttgtaacatc
     9421 tcaatctcat cagcatccaa tgtttcttca ctatttgtca gcatataggc aatttcatca
     9481 cgggtcatct tttcatccgc atcatcgaat gacataggag tcaaatggct caagaaattg
     9541 gtcgaagcag ctaaaagcca aacaaaagga ctgactagtt ttccgatccc aatgataacc
     9601 ggcgctgtac gaattgccaa ggcatccttt agattaagag cgattctctt aggatataat
     9661 tccccaaaaa cgatggaaat ataggtcaaa aatgccaagg atagaaaagt tgccacggct
     9721 tgtgctgttt cgccattccc aagccaagag gcaatcacac gtcctagagt atcagttaaa
     9781 ctcgcccctg ataagattgt aatcagggtg attcctacct gaatggttga taaaaagtgg
     9841 ttaggatttt ctagtacctt cagcaggcgg atgtagcgtc tgtctccttc ttccgccttt
     9901 tgttcaactc gggcacgatt aagagaaacc atggccattt ctgtggctga gaaaaaggca
     9961 tttaataccg tcaagataaa caataaaaca aattgtagca acaaattctg actacttggg
    10021 tcttccatag ttcttctcct aaaatagtat cttatccttt attatatcac aaaatttaat
    10081 ccaatacata ttattttttt cttagcgtaa cttccatctc tctcccaaaa ctggaagtta
    10141 gtctaagacg gatttccact gatttcacgc gtttttctca tactaaaagc taggttgtgg
    10201 gaaactggaa gttagtttta gaagttacta ttttttctta gtacctgttc tagccctgct
    10261 taaactagta aaaagaactc ctacatccat aagtgacata ggagtttagg cttatctttt
    10321 actgagttac agttacttct ccagttcggt aatccagttg aagtcccttt tgaacattgg
    10381 gaactagaac attgaattcc aattctccat ccgaagactt ggcttcaatc tgtgaagctg
    10441 agggaactaa atcctcgttt gaagcgtagt aaagggttac acggtaacgg acacgcttgt
    10501 tttggtccaa ggctttacgc accttgcttt catagtagtt ttgaccagtc gaatactcgg
    10561 cttgtgcctg atttgcccag gctgtctgaa cagcaatgtt tttaggattg cttgttgagg
    10621 catcaaaacc atccaaacca ccgattaagg catagcctaa caaatgacct ctatcgactg
    10681 catgggtata agagcccttt agattcttga cctgatgcca acctggagga gtccaagaag
    10741 ttgaaccatt cccagtttct ttacgattct tgtactgacg agtggcctta gacaagaggg
    10801 cattagctac ggttggaaca gtttccttac ccactgtctt tgttttattg tcagcgtagg
    10861 gcttacttga aaccttggca tctagatttg ttttattacc attgacgata aaagcacctg
    10921 agccattcca ctccagactc ccctttattt gactcttgac tgcgtctgtt aagacactct
    10981 ctgccaatgc ttgactagga gcttcaggcg cttgtttttt ctgactaagc ttggttttag
    11041 gactattagc tgtcgactgc atctgcttga tataatagct ccctgtagac aaaagcaata
    11101 acactagcag tccgattagt gtctgtcttg tttttttgtt catatttctc cttatcttta
    11161 gaaaaaggct ggttctccag cctaatttcc tgtaaattta tgaatcaatt cctgccattt
    11221 tgctggagac aggatagccc atgaatcttg acccttgccc aagattccat aacctaccat
    11281 taaaccgatt cctagagcca gagtacctaa aatcagtact atgatgacta aaagtaaacg
    11341 cttgactacg tagcttgatt tcttattcat cttcatcact tgcctcaatc cgctgaatct
    11401 tagcacctag ctgcgccaac ttctcatgga aaccgtagta acctctatcc aagtgaacca
    11461 atttaccgac cacagtttct ccctgtgcta ccaaacctgt caaaatcaaa gctgcactgg
    11521 cacgaaggtc agttgaaaga acttctgctc cctgcaaagg ctgtccacca acaatacgag
    11581 ctgtatcacg gataatctca gaatgcaagc ccatgcggcg catctcttct aggtgttgga
    11641 aacgattttc aaaaactgtc tccaccatgg ttgattcgcc ttttgcaact gtcatcaagg
    11701 ctgtaaattg agcctgcata tctgttggaa atcctgggtg gggcaaggtt ttcacatgaa
    11761 cagcttttag attttccagt tgagaacgaa cacgaattcc ttcgtcttct tcaattactt
    11821 caacacccat ttcaagtaac ttggcaatca agggacggtt gtgctcccag acagcgtctc
    11881 gaatcaagac atcaccacca gtcatggcag cagctaccat aaaggttcct gcttcgatac
    11941 ggtcctggac tacattgtga gtcgtaccat gaagtttctc aacaccagta atggttatag
    12001 tctctgtacc agcacctttg accttggctc ccatttcatt aaggagaatg gctaagtcaa
    12061 caatctcagg ctcacgcgca gcattctcaa tcactgtcac cccatcagcc agagtcgctg
    12121 ccatcatcaa gttctgcgtt gcaccaacac ttggaaagtc catatagata tgagcaccat
    12181 gcaagcgttc tgcctttgct tcgatgtaac cagctgtctg actaatctta acccccatag
    12241 cttccagacc tttcaaatga agatcaatag gacggctacc aatcgtacaa ccacctggca
    12301 tggatacctt ggcatgaccc acacgggcaa ggattggccc taatacaacg atggaggcgc
    12361 gcatcttact gacatacttg taaggggctt cctcagtgat gtcgccagta gcatccacct
    12421 tgacaagatg agcttcctca tcaaagtcaa ccttggcatt caaaccacca actacctgat
    12481 tcataataaa gacatccgac aaaatcggaa cattctgcaa gacggtcttt ccttcacttg
    12541 ctagaatagt cgctgccaac aagggtaaga ctgcattttt tgctccctcg atcgtcacgc
    12601 ttcctaccag acgattatcg ccaccttgaa ccacaatttt atccatactc gtttccttta
    12661 ctcttgattt tataataatc ctctaagcgc ttcttgccac aaatgataca ggctaataaa
    12721 gaaagaactc aggatgtagc ctatgacaat gctaaaaaag gctactaata cacgaacttt
    12781 tcttgtattc tcagctgtca cttttaaaac cttttcccat ctaacaagat cctttaaaag
    12841 gtaaaaactc acataaataa agagcatatg actacttaga gtgaataata attgaaccat
    12901 ttgactatta taccatcttc tctgtttgtg cgctagtttg gaaacttcac tcaaaaatta
    12961 gggattgttt ttcaagtaat caatcgcatc ttgtagggtt ttaacgggca cgattttcat
    13021 atccgtcttg attgttttag ccgcttctag ggctgtttgg tagttgtttt tcgcgtccgg
    13081 atgcgccttt tgttcttctt cgctaacagg gttatcagga gcaaagaaaa tagcagcacc
    13141 tgccctagcc gaagctacaa ccttcttatc aatacctcca atgtctccca cattaccatc
    13201 gcggtcaatg gtacctgtac cggcaacaat acgaccatta cgaagatctg ggtgagctat
    13261 ttgagtatag atagctagac taaacatgag accagcactt gggccgccaa taccagctgt
    13321 tgaaaagcta attgggacat tgctgattac ctctgtacga tcaatcaagc cgattccaat
    13381 cccatttttt ccattttcca aggtgataat cttcccttct gcagacttgg tttgcccatc
    13441 ctcttcatag gtgaccttga cggaatcccc taatttttga gaactgacgt aatcaatcaa
    13501 gtctttggaa ctatcaaagg tctgatcatt gactgctgtg actgtatcag agatattgag
    13561 aatcccttta aaggttgaat tatccgtcac attcaaaaca taaactccaa agtacttgag
    13621 ttcgatatcc ttaccagctg tttttagtcc ttgatacttg gccatatttt gcgatgtttg
    13681 catgtagaat tgattgattc gcataaattc aacatcggaa gaaccacctg tagtctcctg
    13741 agcactacga atatctgtaa aaggtgtcaa ccaagcataa atcatatgag ctaaagtagc
    13801 atgttgaaca ccaaccgtaa cgaattgata ggcaccagct tccttatctt ctgtgtcatt
    13861 tactttaagg acttggcgaa tatcttccga accacctgga acctctatat aataaggcaa
    13921 gggcactaca aatgccaaga aagtcacaat caaggccgca atgacgtata agggccatct
    13981 aatctttttt ttcatttctt atttcctcca aaatactctc gggaacatag caggcaatat
    14041 cctgaccaaa cttcaaaagc tctctaacgc ctgatgaact gatatagaga tgttcaggtc
    14101 gactatgtaa ataaatagtc tctatatcag aagacagctg atgattgtag taatcaaaac
    14161 tggcttcata ttgcaaatcc gacgcattcc tcaaaccacg cactagacaa gtagcaccca
    14221 atctttttgc aacatcgacc accaattcat catgagaagc cacgacttca acattttcca
    14281 aatgtcccaa agccttttct agcccccgtt tacgattttc aagagggaga aatccttgtt
    14341 tgtggggatt aaaaaaaata cccacacaaa gcttatcaaa aagtctgctc gcccgttcaa
    14401 tgatatccag atgcccattt gtcatcggat caaatgagcc tgtgaataag ccaatcttat
    14461 ctgacataga ctgtcacctt actaattcca taaatctttt ccttccagat acccagacag
    14521 gcaatttctt ctggaagttc aacggtctta tccgtctcgc acacaaccat aacatcttca
    14581 gaaaaaagct ctctctcagc cattttttca atatctgcta cgatttgttc cttggcatag
    14641 ggagggtcta agaaaacgag gtcaaattcc ccagatacct gttccaatgc cctttctgca
    14701 tccattttga ggagttgaaa ttttccaact tccttggtca tctggatatt ttcagccacg
    14761 atggtctgag ccttacggtc tcgctccacc aaaacagcac tggacatgcc acgcgatact
    14821 gcttcgatag ataaaccacc actacctgca taaaggtcca agactcgtcc cacttcaaag
    14881 tagggaccaa tcatgttaaa aatggctccc ctaaccttat ccgaagtagg tcttgttgtc
    14941 ttgccttcta gtgtcttgag gggacgtccc ccatagattc ctgatacgat tttcatactg
    15001 tttattatac caaattatag acaaaaagag aaagaaaacc gaaccttgcg gttcgattct
    15061 ctacaaaata ttttcgtaag tatcgcggac ttcttgaggc caaacacttg tttgcacttc
    15121 tccgatgtgt ctcttgcgaa gtaggaacat ggccatacga gattgtccaa ttcctccacc
    15181 gattgtcaat gggaataggc cattcaacaa agacttgtgc cattccaatt ctaagcggtc
    15241 ttcatcacct gtaatttcca cctgacgtct aagagtttct tcatctacac gaattcccat
    15301 agaagacaac tcaaaggctc cacctaaaga ctcattccag acaagaatat caccatttag
    15361 acccttgtag ccattctcag actcgcttgt ccagtcatca tagtctggtg cacgtccatc
    15421 gtgcggttta ccatctggca actcgccacc gataccaatc aaaaagacgg ctccaaattc
    15481 tttacaaatc gcattttcac gttctttagg tgtcaagtct gggtagcgtt ctaccaattc
    15541 ttctgtatgg ataaaggtga tttgttttgg caagatagac tcgatgtcat agcgggcttc
    15601 aacagctagc tcagtcaggc gaatagcctt ataaatcttc tcaactgttt cttttagata
    15661 aacgatgtta cgcttaccat ttgggataac cttctcccag tcccactggt caacataaac
    15721 agagtgggtt gcatccaagg aatcctcatc tggacgaagg gctttcatgt ggacaaagag
    15781 accctctcct tcaccaaagc caaaacgagc caaggtgtgg cgtttccatt tagcaagtga
    15841 gtgcaccact tcataagtag catcagggat ttggagaacc ttgaccgata ctggattttc
    15901 cacaccagac aggttgtcct gcattccgtc accgacctta ctcaagatag gaccttgaac
    15961 ttcgacaact tctagcttat ctttcaaata ctgggtaaaa gtgtttttga caaaggaaat
    16021 ttcttcttgt tgatggataa aacttttctt catagacaac tcctcaaaaa tttaataaac
    16081 gtattataca cctatttcca agaaaagaaa agagaaaatt ttaaaaaaat cagtgtcact
    16141 caaaacactg atttcataga ccttttagtc attgcgacca aagatacgta gaatacttag
    16201 gaaaagattg ataaaatcaa gatagatgct gagagccatt gacaccaccc agcctgtcgc
    16261 tacacgacct tgtgattgtt cataggcgag gcgaatcttt tggttatccc aagcaatcaa
    16321 ccctgaaaaa accaaaacca tggctacgct aatcatataa tcaaagaagc cactagccaa
    16381 gaaaatattg actaccatag caatcagtaa accaatgaga gctgccatca ttgctcgacc
    16441 aatcccactc aagtctttct tggtaaaaat accaactgcc gccatgacaa agaagagaag
    16501 ggcgctcgat acaaaggcag ataaaactgt acccggagta tagaaagcca ccacaaaact
    16561 aagggtaaag ccgtttaaaa cggagtaaag taaaaatact ggaagagcag ctggactatt
    16621 tcttgaagcc atgctactgg caacgaaaac caaagccaac tccgcaaaag tagcaatggt
    16681 caaccagaga cgcccctgca tcaacaagta aaccaactga gactgaaaga ccgtcaacat
    16741 aaggcctgat accaaagcag atagtccgat tcccagacca acaaaggcat aaaccttagc
    16801 gtaaaattga ttgagacctg cacggtcatg tataatagtg tgattcatcc ttttctcctt
    16861 ttctgtgaac atctttcctt gattataaca aaaaaagtta aaattagctt aaatggaaga
    16921 ttaaaatacc ggctgcaact gcaacattca aactctcggc ctgccccttc atgctaatat
    16981 ggaccaactg gtctgcactt tcagccataa ggggactaat tccttgaccc tcatttccca
    17041 taactagtac aaaattttct atagaaggca gttctctgta atcaacagaa tctttagata
    17101 gggttgttgc tagcactggg atagctacct ttttagtttc gtcaagaagc gcttgactag
    17161 tcatccggta aatgggcaga tggaaatgac taccttgcat ggaacgtagg gttttgagac
    17221 tgtagatgtc tgccgactta tctgaaacaa tcactcctgt aaaacctgct gcatccgcag
    17281 ttcgaatgat agttcctaca ttaccaggat cttgcacatc ttccaaaaac aagaacttgc
    17341 cctgactcaa atcagcttgt cctacttctt ctttttgaac cacggcaaca attccctgtg
    17401 gagtctgaga atccgccaaa tctagcaaaa tatcctctga aatccagaca gtttgcggaa
    17461 aagcagctaa ctgatttcga taattttcta gggcaaagat tttctcaatc gtcactccag
    17521 cttggacagc ttcttcaaac aagtgccagc cttcaatcaa ataggcagac ttgcggtatt
    17581 ttttttggtg taatttcttg gcatttttta ccacagaatt ggcttttgag gttataatag
    17641 tcatagaaat attataacac aatcaaaggg gtttggtatg caaaaggtta gaatgattgc
    17701 ccaaggtagg gtgcagggag tcggctttcg ttggggtgtt tatagcctgg cccttgaaat
    17761 cggtggcatc acaggtcggg tatggaataa cgacgatggc acagtggaaa tcttagccca
    17821 agcagactca tctgctatca tggcaaaatt tatccaagaa atccgaaaag gaccgacacc
    17881 tttttcaaaa gtaagctact tagatgtcaa actaagcaac tttcctccct actctgactt
    17941 taaaatcgca aattaggtct ccagaactat tgtatatttt gtaaaaaaac agtagaatag
    18001 aaaggtataa tttttaaaga aggaataaaa acagtgaaat ctattaaacg ttttgcattc
    18061 tcggctatgg gagtggctat gttgcttgtc ttgactggct gtgtcaatgt cgataaaacc
    18121 acaggtcagc caacaggatt tatttggaat acgatcggag cgcctatggc tgaagccatc
    18181 aagtacttcg ctactgataa aggtctaggc tttggtgtcg ctatcattat cgtaaccatt
    18241 atcgtacgct tgattatctt accacttggt atctaccaat catggaaggc aacgcttcac
    18301 tctgaaaaga tgaacgctct caagcatgtc cttgagccac accaaacacg tctcaaagaa
    18361 gcaactactc aagaagaaaa actcgaagcc caacaagccc tctttgccgc tcaaaaagag
    18421 cacggcatca gcatgtttgg tggtgtagga tgtttcccta tcctccttca aatgcctttc
    18481 ttctctgcta tctactttgc tgcccaacat actgaagggg ttgctcaagc aagctaccta
    18541 ggcattcctc taggttctcc aagtatgatt ttggttgcct gtgctggtgt cctttactat
    18601 cttcaatcgc tcctttcact tcacggagta gaagatgaaa tgcaaagaga acaaatcaag
    18661 aaaatgattt acatgagccc actcatgatc gtcgtcttct ccctcttctc accagctagt
    18721 gtcacacttt actgggttgt cggtggtttc atgatgattc tccaacagtt tatcgtcaac
    18781 tatatcgttc gtccaaaact tcgcaaaaaa gtccgtgaag aactagccaa gaacccacca
    18841 aaagcaagtg ctttctctaa accaagtgga cgaaaagacg ttacccctga acaaccaact
    18901 gctatcacaa gcaagaaaaa acacaaaaat cgcaacgctg gaaaacaacg ttcgagataa
    18961 gaagaaaaag gcttagctga tttgctaagt ctttttgcaa atcaaaagcc cgatgtttcc
    19021 atcaggcttc ttttctatcc atgtacacgt ttcatataat cttgataact ttcggtatcc
    19081 atgagttttt tagcgttctt gacgcgatct gctgttggtg gtttgactcc ttcgagggaa
    19141 tatggaattc caagttcacg ccacttgaac tcacccatgg tgtgataagg tagaatttca
    19201 aacttatcaa catttttgag ggtcttgacg aacttaccaa gttcaatcaa gtcatcatct
    19261 ctgtctgtca atcctggaac tagcacgtgg cgaatccaga caggttttcc aatatctgat
    19321 agatactggg cacaagccaa gatattttta ttagtttggc tagtgacaat cttgtgctgt
    19381 tcttcgttga tttccttgat atccaaaaga accaagtcag tgacagccat gagtttgtca
    19441 aacttctcaa ggtaacgtgg tttattacgg aaaggaagag cacaggtatc caaggtacag
    19501 tggattcctt gttccttagc cttggtgaag agagcaatca ggaaatcaat ctgcaagaga
    19561 gcttctcctc cactgactgt aatcccaccc ttatttcccc agaaaccacg gtagcgcaag
    19621 gcctctgtca agacatcatc taccgtccgt tcacgtgact tattggactc catagcccaa
    19681 gtgtctgggt tgtggcaata ctggcaacgc atgtgacagc cctgcaaaaa gacaataaag
    19741 cgaataccag gcccatctac tgacccaaag ctttctgtcg aatgcaccat tcctgtcact
    19801 tgtccataat caattgtttc ttcagacata tcgttacctt ccttgaaaac gttttatagt
    19861 tttattatat cacgacttga aggaaaaaca agatttttgc ctatttttta gaaagcaatc
    19921 tgtttttcaa tttcattttc aatctattat cagattattc ttcaaaatcg aagccatata
    19981 aggttgcaaa atagtcctca gggcgctctg cacggcggat ttgacgggct ttaccttctt
    20041 cggtatagag gatttccgca gaacgtaatt tggcattata ctggtagccc attgaaaatc
    20101 cgtgggcacc tgtatcatga attaccagca aatcaccgat ttctgtatga ggcagttcgc
    20161 gattgactgc aaatttatca ttgttttcac agagtgaacc aaccacatct accgcttcag
    20221 ctggtccatc tggatgggtc acgttgctaa tatgatggta agctccgtac atagctggac
    20281 gcatgaggtt gactgctgag gcatccacac ctagataggt acggtaggtt tccttcttat
    20341 gagtcactct tgtgactaga gcaccgtgag gtgccagcat aaaacgaccc aattcggtga
    20401 aaatcttgac ctgaccaaga cctgctgacg taagaacttc ttcatacacc ttacgaactc
    20461 cctcaccaat caaggcgata tcgttcggct cctggtctgg acgataatta acaccaatac
    20521 cgccagaaag attgataaag tctagcgaaa tgcccaactt ttccttgatt tcaacagcca
    20581 gttcaaagag ctgacgagcc aactctggat aatagagatg ggtcacggta ttggacgcta
    20641 ggaaggagtg aatcccaaaa gtcttggctc ctttttcctt caagatagca aaggcttcaa
    20701 agagctggtc cttggtcatg ccaaacttag cctccccagg attgtccata atgtctgtcc
    20761 ccagttcaaa aacgcctcca ggattataac gacaagagat gatttctgga atgcctgcta
    20821 ctctctccag atgttcaata tcttcaaagg catccaagtt aatggtcgca cccaattcac
    20881 gtgcataggc gtattccttg tctggcgtgt tgttggaaga gaacataatc tcagaaccca
    20941 gaaagtccag tttatggctc atcaaaagct ctacataact agagcagtcc acaccacaac
    21001 cttcttcttg gagaattttc aaaatagctg gagttggagt agccttaact gcaaaatatt
    21061 ccttaaagcc cttgttccac gaaaaagctt ggttgacggc tcttgccttc tcacgaatcc
    21121 ccttctcatc atacaagtga aagggagtcg ggaactcggc aacaatcgct tctaactctt
    21181 ctctattgat aaatggtgtt ttcataagct tccttctatt ctgtttgcaa agaaaggctg
    21241 ggacaatcgt tccaaccttt agttctatct cttatttatc ttcgtcaaag atattgccaa
    21301 cctcaacatc gatggcttgg ttcttgacat caatattggt taccttcaag agtgacttgt
    21361 agtcaaactg cttttcacgt tcttcttggg cattgtccgc aaagaccgct acacctgcca
    21421 gttctgagtc gaactcgtgc aagagactaa tcataccatt gaccgttccg ccacctttca
    21481 agaagtcatc cacaatcaag acacggctgc ctgccttaag actacgtttt gaaaggaaca
    21541 ttttctcgat acggtcacca cttgaacctg aaacatagtt gacgctaaca gttgaacctt
    21601 cggtaatttt caggtcacgg cgcacaatga caaaagagac attgaggaca ttggcaactg
    21661 catttgcaag tggcacaccc ttagttgcta cggtcataac cgcgtcaatt ttttggtcca
    21721 taaagctttt ggcaataata cgaccaatat ttttcaagat ggctggtgtg ctaagcaaat
    21781 cagacagata gatataacca cctggcaaga tacggtcact ttctgacaac ttggtacgca
    21841 agtcttcaac catttcctta gcatcctgac tcgaaataga cggtgtgaag atgacacctc
    21901 cgccagcccc agtcactgtc tggatatgac cgatttcaat ttcctcaaag gcgcgtttga
    21961 taatgacgat atcttctgag atggatgatt tagcagactc atacttttca gcaaaagtat
    22021 tgagactagt tagtttataa ggattattaa tcaaatagtt ggaaatgaca accatccgat
    22081 cacttcttct taatttcatt tctatttcct attccatttt tattcaaaaa atcaaaaagc
    22141 aaactagaaa gctggtcgct ggtggttcaa aacactgttt tgagattgtc aatagaactg
    22201 acaaaccctg taatatacct gcatatatac atacgacaag gcgatactac cctagtttga
    22261 agagattttc gaagagtatt catttttgtc ttttacttat tataccatat tcacataaaa
    22321 aaacgaacat tcttatccta aaaaatgctc atttttctta aattatcaat ctaaatctgg
    22381 tttatagaag gaacgattat ccatagcgaa gattttattg gtcatctctc ctttatccac
    22441 caaagccaga gctgttgaca tcatcatcat gcttgcatcc agattgtcaa tcatatggat
    22501 aatctctgcc tccataatac gtggacggac tggacttcca tattcaagca agccgtggtg
    22561 actgaggatg acatgacgaa gcaaaacgac ttcttccttg gtatcatcga tgccgagttc
    22621 cataactgtc ttggtaattt cgctatcaat gagagcgata tgtccaagaa gattacctcg
    22681 cactgtgtac tctgtctggt ctggccccgt caactcgata accttagcta agtcatgcag
    22741 cataatcccc gcatagagca ggctcttatt gagctgagga taaacttcgc taatagcgtc
    22801 tgccaaacgt accatggtcg ccgtatgata agccaacccc gtttcaaagg catggtggtt
    22861 ggtcttggca gctggatagg agtagaattc cttatcatac ttggtgtaga gatttcggac
    22921 aatccgttgc cagacaggat tttcaatttt gaaaatcatt tgcgacatgt agtcacgaat
    22981 ttccttgaca tcaactggtg acttgacctt gaaatcggct gggtcattgg gttcaccagc
    23041 ttgaggcagg cggagagtaa tttgattgac ttgaggggta ttgttataaa cttctcggcg
    23101 tcctttcatg tggacaacct tacctgcggt aaaggcctca atgttatgag gttgggcatc
    23161 ccagagcttc ccatcaatct cgccactatc atcttggaag gtaaaggcta ggtagttttt
    23221 cccagctcga gtttgcctca ggtcagctga tttgattagg taaaagcctt caaatagctc
    23281 atcttttttc atgtgactaa tcttcatatt cttcctcatt ttcttgaaaa tggagtagat
    23341 caagcgcagg ctcaccttct gacaactcaa tgtgacggag cgtccgctcg atagctatgg
    23401 tacgacggtt taataattca tcaatattgc cagaggcatg ttggagatgt ttttgtgcct
    23461 tgaccagaat accaccaaac ttgccaaact cggtcttgac actggcaaga gtcttgctga
    23521 tatggtcggc actcttttgg atattaaggg tcttgaaacc aactgataga gagttaagaa
    23581 gggctgatag ggtacttggt cctgcaacaa taatctgttc ttcccgtctc aaatcatcaa
    23641 agaagaccgg attgcgcaca atctctgaat aaagaccttc tgtcggaaca aacaagattc
    23701 caaagttggt tgtccgaggt ggtgctatgt acttgttcct aatatcccta gcaaagcgct
    23761 tgacgcttgc taggagtgac ttacgacagc gttcaatctc atccttgtca cctgtctcat
    23821 aggcttcttc caagcggtaa taatctgcca gtggaaactt agagtcaatt ggcagataga
    23881 cgtattcttg gtcgccttgt ccgggtaact tgatggcata ctccactcgt tcactagagt
    23941 tttcaaccgt tgcgtattct cgttcgtact gggcaggtgt catgatgtct tcaataattt
    24001 gccccagttg caattctccc agaatccctc gcgtcttggt tccagagaga accttgttaa
    24061 gagctccgac atcacgggca actgtctgca tttctccaag gccacgattg acagactcca
    24121 gttgtttaga aactgtctca aaggaagcct gtaagcgtgt ctgcaaggtc ttttctagtt
    24181 tttcctcgac cgtctggcgc atttgttcca aacgttgctc atttgattcc tgcaaggctt
    24241 ggagacgttg gtctgtcttg tctctagttt ggaggagatt atctgtcatt tcttgacgga
    24301 cttgggtcag accttggtgc aattcaatcc gcacttcttg caaacggtcg ctgacaacca
    24361 cttccaaatc tttttggtct aactggctgg cttgtctggc ttggtcaaag cggtaatcca
    24421 actggtctga caaatgatct gcctgatcct ccaagctctt acttaagtgt ttctcctgcc
    24481 tatcctgcct ttgccaaatc agaaagagac cagctagatt ggcaattaat aatagaataa
    24541 gtaaactttc catcctacct cctgtccttg ctatgcagta cgaccacata accatctggg
    24601 caagttaccg acacttccct atctatatat tcgttagaag cgtacacttt tttaaagaaa
    24661 aaattttcct ctgtcaactc atacttggct ccaagaatag tcagctggct atcccgaact
    24721 ggcataaagg ctagatagtc gtagtctgaa cgaggttcta gctgactgat tccttctgga
    24781 caataagtaa tcaagttttg cccatcctca atttctattt gatgcatata ggatgccaac
    24841 ttaggattgc taggcagaaa gacattggcc aacatatggt caatacggcc acccaaggca
    24901 ccgaaaatag taacctgagc ctgaggattt tgcttaaaga tggtcaagag agccaattcc
    24961 aaatcagtat catctttttc tggccgggct tggacaaaat actgggcacg tttttgaatc
    25021 acctgtcgct cttcttccgt cacagaatca aaatctccga ctgctagagc aagaggcaaa
    25081 tcttcttcca agatccagag cgagcctcga tccacaccaa caaaaccatc aaagtccgtc
    25141 cgataatgac cgcggtctcc gcctgcaaaa acggcaaccc tagtccagtt gttttctgag
    25201 agtttgtact cgctcattga catctccctt aaagacatag gaacctgcta caaaaacggt
    25261 cgcaccagct tctttggctt gagcaatagt ttggtcatca atcccaccat ccacttcaat
    25321 ctcaaagttc aaaccttttt catcacgaag ggcaaccagc tcacgaacct tatccatggt
    25381 ttctggcaga aaggcttgac caccaaatcc agggttaact gtcatgacta ggacttggtc
    25441 aactagatga agcacatgct taatcgcttc aactggtgta ccaggattga taacaactga
    25501 tggcttgaca ccgagtgaac gaatcttttg gagggcacca tgaatatgag gcgttgcttc
    25561 cacatggata ctaataatat ccgcacctgc acgcacaaag tcctctaaat gatgctcagg
    25621 gtttgacacc atcaagtggc agtcgaaaac catcttacta tgaggacgaa gagcttcgac
    25681 cacacccgca ccaaaactga tttgcggcac aaaatgacca tccataatat cgatatgggc
    25741 atactctgcc ccagttgctt ctaggcgttt aatttcacgt tcaaagttgg cataatctgc
    25801 tgccagaatt gacggagcaa tcttgtattg agacataggt ttctccttat tttggaattt
    25861 ttttgctgac ttttttatag gtttctctgc gattttcaat ttcgctgagg aattgcaggt
    25921 agttgtcaaa acggaagggc gcaatggtac cttcttcaac tgctggcttg acagcacagg
    25981 acggctcatg ggtatgggta caagtacgga atttgcagtc tcgactgaca gtggcaatct
    26041 ctggaaaggc ctgattgagg tcttcagccg ttgatacttc ataatccagt gatgaaaagc
    26101 ctggtgtgtc tgcgatttta cccccattga ggttgtaaaa actaacagct cgagtggtat
    26161 gacgcccgcg acccagactg tctgaaattt ctcccgtttc aagattaaga tctggtgcga
    26221 ttttattgag aagagttgat ttcccaacac ccgtctgccc cataaagacc gtaaccttgc
    26281 ctgttaacaa aggcaggagt tcctctttac tggtcacaaa gtcatatccg atgtcaccat
    26341 aggtctgctg gtaaaaatcc agttctcccc cgtcttccaa caaatccatt ttagaaatat
    26401 agacgatggg atgaataccc ttatgctcca aaagaaccaa gaaacgatcc agcaaattgc
    26461 tgttaaaatc aggttccttg acagacatga ttactacagc ttggtcgata ttgacaatag
    26521 gcggacggac cagactgttt ttccgttcgt gaatcttgag aatatagcct tctgaatttt
    26581 cctcggcaga gaaatctacc cagtccccaa cgtaaggggt atgccctttt ttacggaaat
    26641 tcccacgcgc gcgtgtttga tagacttggc catcactctc tacatagtag aacccagcca
    26701 aggctttaat gatttgtccc tgcaaaataa ctcctttaaa attgttagtt ccattataac
    26761 aaaaaaaagc tattttagct tagcttctct ttacaatttc gataagtcta ttctaaaaga
    26821 aaaagataca ctcacgcttt agataccaag ttttatacaa aaataacgaa tctacctcct
    26881 tatataaaaa caggcgaaca gagagcctaa aactccctgt tcgctttact ttttctgtgt
    26941 gtaatagaaa aaacctaata aagcaagaca aaaaatatga atataaactc catacatcat
    27001 gtggggaaag acagactcta taaaatagcc tggtagatag atgattggaa attgatagaa
    27061 atcataggtc gttagagata gtaaacaaag aacatacata aatactgcta caattagaac
    27121 ccatccttgc tttcgccata gaaaaatcat caataagaac agacaaagta gatagattga
    27181 ccaatttcgc aacataaatt gaaaaccata aatcatactt tcagtcgttt ctccatcaat
    27241 cacatcaact actttataga aaggtaaaca atagctcact aaaaaaagaa ggggactaat
    27301 tatttttttc atattctcta ccatggatta ttttgcaagt aagctgacca tctatctcca
    27361 tatcgaatca aattaacagt ataattagtt actacccgat tttcacctgg tccaacctta
    27421 gaaaaacctg tgctatactc ccaactactt cttgcacctg gatgaaacca tttttgctgg
    27481 gatgaatacc aaccaccttg tctaacttcc acacttgaag attctatttc gatatccctt
    27541 tcatggaagt caactcctcc acttatttta gtaatatttt tttgtacacc tccatcataa
    27601 ctcacccagt gaacaacagt atacaattcc acactataag tactatcaaa ctgactttga
    27661 tgagtagtaa tgttttgagt ataagcgtgc actttctgta taccaaaaat atggtctaat
    27721 atattcgttg gtttagcaag cttattgtaa ccctcttcaa caaaagtagc cttctctaaa
    27781 tcagcggtat aaacagtttt tccttgtact acttgattgt ttatagaagt aatacggcta
    27841 tccacaacaa tgggggccac catatctcca tcgctagttt ctattttagc tgcctctttc
    27901 acctcatata caactcgatt ttcttcagac tgaatcactt ctaccccatt tttttcaggt
    27961 atagataatc tagtttgccc attagaaacc gttatatttg gaccaatatt cttctcattt
    28021 gcataagtcg caataggaaa tcctgataga accactgcac acataaggct attgactaat
    28081 ttttttctca tgaatatatc cttcccattt tcaagaggag taaaaatagc taatataaga
    28141 tgaacgtttc cttatatttc agaataggat atctatctga aagcaattca cactcctata
    28201 gataactgtt ccaaatcttg gctacatata tttcaatagt ttgctacaaa aaactccttt
    28261 ctaagtctat tgaacatgcc cttattcacc tactaatata tcacaaaaat aaataaatac
    28321 aagactgttt tttctactag ttttatgata gaatagtatt gttagggtaa atttcatata
    28381 taaatgtcga gtgaaaaatc gatggagcag aaatcggtag acaaagactc gatttcgttg
    28441 gtccagcccc acaaggctaa tcaggtattt cacgagttta aaaatctaat gaagtgccca
    28501 atcaactact gattcattga tttaatacga actatagtgg attgaaccta gaaatagtac
    28561 actaaggatt ctaaaacgtt tctagaaatt aatttgactt tcctaatctc tttgttcata
    28621 tcttatttca atctactata tataccaaaa tgctaggaca aaagtcttta aaatcagaga
    28681 aacgcatatt atcaggtgtt ggaaaacctt gataatatgc gttttatcat agaaagatct
    28741 acttcatttt ttactaaaat taagtttttg tccctgcatc ttagagtcct tgccctttaa
    28801 gtgcatctgc taggctggca aactcttcca agctgagagc ttccccacgc acacttggtg
    28861 acaagcctgc ttggtccaaa gccttggtca gcttgtcctt gacctcttca gtcttgccaa
    28921 agtaacctgt caagttattc cacaaggtct tgcggcgatg ggtaaaacta gccttggaaa
    28981 ccttaaagaa aaagttctcg tcttccactg ctacagctgg cactggacgg cgcaccattt
    29041 tcaagatggc tgagtctaca tttggtgctg gcacaaagac tgtacgcggc acgataaagg
    29101 caaccttggc tgtcatgtaa tactgcacag cgatagacaa gctaccgtaa gccttggtat
    29161 ttggttgagc cgagatacgg tccgccactt ctttttgcat catgaccaca aactcactaa
    29221 aaggaatgcc actctcgatt aagtgcatga gaataggcgt tgtgatgtag taaggcaaat
    29281 tagccactac cttgattggc aggtcaggat ttttgaaatt ctggatatgt tgcgccaaat
    29341 cgaccttgag aatgtcctcg ttgactacag tcacattgtc aaaatcacgc aaggtatctg
    29401 ccaaaatcgg taccaaacga tggtcgatct caaaggccat aacttctgct gcacgctcag
    29461 ccaaaaactc ggtcaaggca ccaatacctg gcccgatttc aatgacattg acctggtcat
    29521 caatctcagc cgtatccacg attttttgta ggatattggt atccgtcaag aaattttgcc
    29581 caaaggactt tttaaaggta aaaccgtgac gctccagcac tgccttggtc acgctataat
    29641 ctgcaattct catctattct cttttctatt atctgttact actattgtaa cacattcccc
    29701 ttgtattttg ggggtgaaaa tcataccaaa aaactatctt tcctcatgaa gaaagatagt
    29761 cttggaaatc tttaaaagtg gccttagcgc ttctctcgaa acatacgtt
//
LOCUS       DAVBHS010000008        29513 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVBHS010000008 DAVBHS010000000
VERSION     DAVBHS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.008"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            182..412
                     /gene="blpU"
                     /locus_tag="VQ651_000242"
     CDS             182..412
                     /gene="blpU"
                     /locus_tag="VQ651_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HET2753291.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
     gene            1127..3280
                     /locus_tag="VQ651_000243"
     CDS             1127..3280
                     /locus_tag="VQ651_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HET2753292.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            3293..4642
                     /locus_tag="VQ651_000244"
     CDS             3293..4642
                     /locus_tag="VQ651_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET2753293.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAMETGASLASQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4813..5520
                     /locus_tag="VQ651_000245"
     CDS             4813..5520
                     /locus_tag="VQ651_000245"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET2753294.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            5722..9447
                     /locus_tag="VQ651_000246"
     CDS             5722..9447
                     /locus_tag="VQ651_000246"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET2753295.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVTKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9540..10982
                     /locus_tag="VQ651_000247"
     CDS             9540..10982
                     /locus_tag="VQ651_000247"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET2753296.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            11019..12041
                     /locus_tag="VQ651_000248"
     CDS             11019..12041
                     /locus_tag="VQ651_000248"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET2753297.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            12038..12583
                     /gene="purN"
                     /locus_tag="VQ651_000249"
     CDS             12038..12583
                     /gene="purN"
                     /locus_tag="VQ651_000249"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET2753298.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIALVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12667..13176
                     /locus_tag="VQ651_000250"
     CDS             12667..13176
                     /locus_tag="VQ651_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET2753299.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLIFFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            13201..14748
                     /gene="purH"
                     /locus_tag="VQ651_000251"
     CDS             13201..14748
                     /gene="purH"
                     /locus_tag="VQ651_000251"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET2753300.1"
                     /translation="MTKRVLISVSDKADIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYDDAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGENKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14870..16132
                     /gene="purD"
                     /locus_tag="VQ651_000252"
     CDS             14870..16132
                     /gene="purD"
                     /locus_tag="VQ651_000252"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET2753301.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVSETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFFEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNITWTDKGVTLGVVVA
                     SKGYPLNYSKGVELPVKTDGDIITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            16535..17023
                     /gene="purE"
                     /locus_tag="VQ651_000253"
     CDS             16535..17023
                     /gene="purE"
                     /locus_tag="VQ651_000253"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET2753302.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            17010..18101
                     /gene="purK"
                     /locus_tag="VQ651_000254"
     CDS             17010..18101
                     /gene="purK"
                     /locus_tag="VQ651_000254"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET2753303.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            18111..18338
                     /locus_tag="VQ651_000255"
     CDS             18111..18338
                     /locus_tag="VQ651_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HET2753304.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            18401..19699
                     /gene="purB"
                     /locus_tag="VQ651_000256"
     CDS             18401..19699
                     /gene="purB"
                     /locus_tag="VQ651_000256"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET2753305.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19754..23692)
                     /gene="strH"
                     /locus_tag="VQ651_000257"
     CDS             complement(19754..23692)
                     /gene="strH"
                     /locus_tag="VQ651_000257"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET2753306.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEDDKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKESARTVNLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGSMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQTLNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
     gene            complement(24007..24723)
                     /locus_tag="VQ651_000258"
     CDS             complement(24007..24723)
                     /locus_tag="VQ651_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2753307.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            25071..26858
                     /locus_tag="VQ651_000259"
     CDS             25071..26858
                     /locus_tag="VQ651_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET2753308.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPVYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            26855..27331
                     /locus_tag="VQ651_000260"
     CDS             26855..27331
                     /locus_tag="VQ651_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET2753309.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            27359..28264
                     /locus_tag="VQ651_000261"
     CDS             27359..28264
                     /locus_tag="VQ651_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2753310.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            28251..29066
                     /locus_tag="VQ651_000262"
     CDS             28251..29066
                     /locus_tag="VQ651_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2753311.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            29073..29477
                     /locus_tag="VQ651_000263"
     CDS             29073..29477
                     /locus_tag="VQ651_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2753312.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
BASE COUNT         8121 a         5763 c         6954 g         8675 t
ORIGIN      
        1 aaaaaagtga ttagaaaaca tcttttttaa aaatagagat gattttgaaa caaaaaagct
       61 aattcaagac gtttcgatgc caattcaaga tttggatgaa aaaaattaat agatactgtt
      121 atactaaact tgtcaagttt gtaacaagac aaatattaaa aataaaaaag aggtattcgt
      181 tatgaataca aaaaagatgt cacaatttga aattatggat actgagatgc ttgcttgcgt
      241 tgaaggtggc ggatgcaatt ggggagattt tgccaaagca ggtgttggag gaggagcagt
      301 acgaggtctt cagctaggaa ttaaaacaag aacatggcaa ggtgcagcaa ctggtgctgc
      361 gggaggagct atacttggag gtgtggccta tgcagcgaca tgttggtggt aattatggat
      421 tttaaaagtt ttattattgg tttagtagtt ggtatatttg gtccttatat ggatgattta
      481 attagaaaaa aatttttaaa gtcttcggag aagaaaacag aaaaatctgt taaaaaataa
      541 tcaaaactat aaatgatgaa tctgaatcaa aattattttg cgcatgtaaa gaggagtctt
      601 atagtaacga gtcaaaaaag gagtaactat gaatcgtaat ttagaacggt gttatctatt
      661 ctgactagga atagatcata ccagaggtag cttagaaata gcagggacat tagaaattga
      721 agtaataaat aggatgtcgt aagtgttact atcaatgatt tatttgtttc aagcttgcct
      781 agggtgacag taaaaaatca atttcctttc aatagcatat ttttagtggg caggactctt
      841 gttctgccta tttttttatc caaaaagtgc agttgggagg gagataggct catttgggaa
      901 ggaagtccag tttttgttta gtgattgggg taagatagtt gttatcagat gagtttatac
      961 tcttcgaaaa tcaaattcaa accacgtcaa cgtcgccttg ccgtatatat gtgactgact
     1021 tcgtcagtcc tatctacaac ctcaaaacag tgttttgagc agcctgcggc tagtttccta
     1081 gtttgctctt tgattttcat tgagtattag ggaaaaggag atgaatatga aatttgggaa
     1141 acgtcactat cgtccgcaag tggatcagat ggactgcggt gtagcttcat tagccatggt
     1201 ttttggctac tatggtagtt attatttttt ggctcacttg cgagaattgg ctaagacgac
     1261 catggatggg acgacggctt tgggcttggt caaggtggca gaggagattg gttttgagac
     1321 gcgagccatt aaggcggata tgacgctttt tgacttgccg gatttgactt ttccttttgt
     1381 tgcccatgtg cttaaggaag ggaaattgct ccactactat gtggtgactg ggcaggataa
     1441 ggatagcatt catattgccg atccagatcc cggggtgaag ttgactaaac tgccacgtga
     1501 gcgttttgag gaagaatgga caggagtgac tctttttatg gcacctagtc cagactataa
     1561 gccttataag gaacaaaaaa atggtctgct ctcttttatc cctatattag tgaagcagcg
     1621 tggcttgatt gccaatatcg ttttggcaac actcttggta accgggatta acattgtggg
     1681 ttcttattat ctgcagtcta tcattgatac ctatgtgcca gatcagatgc gttcgacact
     1741 agggattatt tctattgggc tagtcatcgt ctacatcctc cagcaaatct tgtcttacgc
     1801 tcaggagtat ctcttgcttg ttttggggca acgcttgtcg attgacgtga ttttgtccta
     1861 tatcaagcat gtttttcacc tccctatgtc cttctttgcg acacgcagga caggggagat
     1921 cgtgtctcgt tttacagatg ctaacagtat catcgatgcg ctggcttcga ccatcctttc
     1981 gattttccta gatgtgtcaa cggttgtcat tatttccctt gttctatttt cacaaaatac
     2041 caatctcttt ttcatgactt tattggcgct tcctatctac acagtgatta tctttgcctt
     2101 tatgaagccg tttgaaaaga tgaatcggga taccatggaa gccaatgcgg ttctgtcttc
     2161 ttctatcatt gaggacatca acggtattga gactatcaag tccttgacca gtgaaagtca
     2221 gcgttaccaa aaaattgaca aggaatttgt ggattatctg aagaaatcct ttacctatag
     2281 tcgagcagag agtcagcaaa aggctctgaa aaaggttgcc catctcttgc ttaatgtcgg
     2341 cattctctgg atgggggctg ttctggtcat ggatggcaag atgagtttgg ggcagttgat
     2401 tacctataat accttgctgg tttactttac caatcctttg gaaaatatca tcaatctgca
     2461 aaccaagctt cagacagcgc aggttgccaa taaccgtcta aatgaagtgt atctagtagc
     2521 ttctgagttt gaggagaaga aaacagttga ggatttgagc ttgatgaagg gagatatgac
     2581 cttcaagcag gttcattaca agtatggcta tggtcgagac gtcttgtcgg atatcaattt
     2641 aaccgttccc caagggtcta aggtggcttt tgtggggatt tcagggtcag gtaagacgac
     2701 tttggccaag atgatggtta atttttacga cccaagtcaa ggggagatta gtctgggtgg
     2761 tgtcaatctc aatcagattg ataaaaaagc cctgcgccag tacatcaact atctgcctca
     2821 acagccctat gtctttaacg gaacgatttt ggagaatctt cttttgggag ccaaggaggg
     2881 gacgacacag gaagatatct tacgggcggt cgaattggca gagattcgag aggatatcga
     2941 gcgcatgcca ctgaattatc agacagaatt gacttcggat ggggcaggga tttcaggtgg
     3001 tcaacgtcag agaatcgctt tggcgcgtgc tctcttgaca gatgcgccgg tcttgatttt
     3061 ggatgaggcg actagcagtt tggatatttt gacagagaag cggattgtcg ataatctcat
     3121 ggctttggac aagaccttga ttttcattgc tcaccgcttg actattgctg agcggacaga
     3181 gaaggtggtt gtcttggatc agggcaagat tgtcgaagaa ggaaagcatg ctgatttgct
     3241 tgcacaggga ggcttttacg cccatttggt caatagctag aaagaggaga ggatgaaacc
     3301 agaattttta gaaagtgcgg agttttataa tcgtcgttac cataattttt ccagtagtgt
     3361 gattgtaccc atggcccttc tgctcgtgtt tttacttggc tttgcaactg ttgcagagaa
     3421 ggagatgagt ttgtccacta gagctactgt cgaacccagt cgtatccttg caaatatcca
     3481 gtcaactagc aacaatcgta ttcttgtcaa tcatttggaa gaaaataagc tggttaagaa
     3541 gggggatctc ttggttcaat accaagaagg ggcagagggt gtccaagcgg agtcctatgc
     3601 cagtcagttg gacatgctaa aggatcaaaa aaagcaattg gagtatctgc aaaagagcct
     3661 gcaagaaggg gagaaccact ttccagagga ggataagttt ggctaccaag ccacctttcg
     3721 cgactacatc agtcaagcag gcagtcttag ggctagtaca tcgcaacaaa atgagaccat
     3781 cgcgtcccag aatgcagcag ctagccaaac ccaagccgaa atcggcaacc tcatcagtca
     3841 aacagaggct aaaattcgcg attaccagac agctaagtca gctatggaaa caggtgcttc
     3901 cttggccagt cagaatctag cctactctct ttaccagtcc tacaagtctc agggcgagga
     3961 aaatccccaa actaaggttc aggcagttgc acaggttgaa gcacagattt ctcagttaga
     4021 atctagtctt gctacttacc gtgtccagta tgcaggttca ggtacccagc aagcctatgc
     4081 gtcagggtta agcagtcaat tggaatccct taaatcccaa catttggcaa aggttggtca
     4141 ggaattgacc cttctagccc agaaaatctt ggaggcagag tcaggtaaga aggtacaggg
     4201 aaatctttta gacaagggga aaattacggc gagtgaggat ggggtgcttc atcttaatcc
     4261 tgagaccagt gattctagca tggttgcaga aggtgcccta ctagcccaac tttatccatc
     4321 tttggaaaga gaagggaaag ccaaactcac agcctatcta agttcaaaag atgtagcaag
     4381 aatcaaggtc ggtgattctg ttcgctatac tacgactcat gatgccggga atcaactttt
     4441 cctagattct actattacaa gtattgatgc gacagctact aagactgaga aagggaattt
     4501 ctttaaaatc gaggcggaga ctaatctaac ttcggagcag gctgaaaaac ttaggtacgg
     4561 ggtggaaggc cgcttgcaga tgattacggg caagaaaagt tatctacgtt attatttgga
     4621 tcaatttttg aacaaagagt aatgttcgtg tttttagagt taaataattt ttaaactgtg
     4681 agaaagattc ttcttgcagt tttttcttta caatttttaa aaaaacatct gctatttatt
     4741 cggttaaatt cttgtgtttt ttggtttttt gtggtaaaat gtgctcaagt aatacgaaag
     4801 gcgaacttta aaatgtcaaa acaattgatc tattcgggaa aagctaaaga tatctataca
     4861 actgaggatg aaaatcttat tatttcaact tacaaggacc aggcgactgc tttcaacggt
     4921 gtcaagaagg agcagattgc aggtaaggga gtcttgaata atcagatctc atcttttatt
     4981 tttgagaaat taaatgcggc tggtgtggcg actcactttg tggagaaact ttcagacacg
     5041 gaacaactca ataaaaaggt taagattatt cctttggaag tcgtgctccg caactatact
     5101 gctggttcct tttcaaaacg ttttggtgtg gatgagggaa tcgccttgga gactccgatt
     5161 gtcgaatttt actacaaaaa tgatgatttg gatgatccat ttatcaatga tgagcatgtg
     5221 aaattcctac agattgcggg tgaccagcag attgcctact tgaaggaaga aacgcgtcgt
     5281 atcaatgaac tattgaaagt ctggtttgct gagattgggc ttaaattgat tgactttaag
     5341 ctagagttcg gttttgacaa ggatggcaag attatcttgg cagacgaatt ttcaccagat
     5401 aactgccgct tgtgggacgc tgatggcaac cacatggata aggatgtttt ccgtagagga
     5461 ttgggagaac taaccgacgt ttatgagatt gtttgggaaa agttgcagga attgaaataa
     5521 tctgtttgca acggaaaacc ttcgtctctc aactaaaagg actcaggctg aaaaggtccc
     5581 ccagaccttt tcactctgta gagaactagg tgaactaaca gatgtttacg aaattgtctg
     5641 ggaaaagttg cagggtttaa aataacaacc tcaaggctgt ttgggaatat tgcaagagct
     5701 gaaataaagg aataagaatt gatggataaa cgtatttttg ttgaaaaaaa ggctgatttt
     5761 caggtcaagt cagagagttt ggttagagag ctccagcaca acttgggact gtcaagcttg
     5821 aaaagtattc gtattgtgca agtatatgat gtatttgact tggctgagga cttgtttgca
     5881 cctgcagaga agcacatttt ctctgagcag ggaaccgacc atgttttaga tgaagtatct
     5941 gtgcaggcgg atcttgctaa ctatgctttc tttgccattg aaagtctgcc agggcagttt
     6001 gaccagcgtg cagcttcgtc acaggaagcc ttgcttttgt tgggaagttc gagtgacgtg
     6061 acagtcaaca cagcccaact ttacttggtg aataaagata ttgatgcgac tgagttggaa
     6121 gctgtcaaaa actacctgct caatccagtt gattctcgtt tcaaggatat cacgacaggg
     6181 attgccaagc aggagttttc agagtcagac aagaccattc caaaattgac tttctttgaa
     6241 agctatacag cagaagactt tgctcgctac aaggccgagc aagggatggc catggaagtg
     6301 gatgatttgc tctttatcca agactacttt aagtcaatcg ggcgcgtgcc aactgagact
     6361 gaactcaagg ttttggacac ttactggtct gaccactgcc gtcatacgac ttttgagaca
     6421 gagttgaaac acatcgactt ttcagcttct aaatttcaaa agcaattgca gtcaacctat
     6481 gacaagtata ttgccatgcg cgaggagtta ggtcggtctg aaaaaccaca aaccttgatg
     6541 gatatggcga ctattttcgg tcgttatgag cgtgctaacg gacgattgga tgatatggaa
     6601 gtctctgacg aaatcaatgc ctgctcagtt gaaattgaag tggatgttga tggtgtcaag
     6661 gaaccttggc tcctcatgtt taaaaatgaa acccacaacc atccaacaga aattgagcca
     6721 tttggtggag cggctacctg tatcggtgga gctattcgtg atccgttgtc aggccgttcc
     6781 tatgtttacc aagccatgcg tatttcaggt gctggtgata ttacagcacc gatttcggaa
     6841 actcgtgctg ggaaattgcc acaacaagtc atttctaaaa cagcagctca tggttattct
     6901 tcatatggta accagattgg gcttgccaca acctacgttc gtgaatactt ccacccaggc
     6961 tttgtagcta aacgtatgga acttggtgcc gttgttggtg cggctcccaa gggcaatgtt
     7021 gtccgtgaaa aacctgaagc aggtgatgtg atcatccttc tcggaggcaa aacaggtcgt
     7081 gatggtgtcg gtggtgcgac gggctcttct aaggttcaaa cagttgagtc tgtagagact
     7141 gctggtgctg aggttcaaaa aggaaatgcc atcgaagaac gcaagattca gcgcctcttc
     7201 cgtaatggca atgtcactcg tctgatcaag aagtccaatg actttggggc aggcggcgtc
     7261 tgtgtggcta tcggtgaatt ggcagacggt cttgaaatcg acctcaacaa ggtgcctctt
     7321 aaataccagg gcttgaatgg tacagaaatt gccatctctg aatcacaaga acggatggcg
     7381 gtcgtggttc gtcctgaaga tgtggatacc ttcgttgccg aatgtaacaa agaaaatatt
     7441 gatgctgttg tggtggcgac agtaactgaa aaaccaaatc ttgtcatgca ctggaatggt
     7501 gagacaatcg ttgacttgga gcgtcgtttc cttgacacca atggtgtgcg cgtggttgtc
     7561 gatgccaaag ttgtggacaa ggatgtcaaa ctcccagaag agcgtcaaac atctgctgaa
     7621 acactggaat cagataccct tacggttcta tctgacctca accatgcaag tcaaaaagga
     7681 ttacagacta tctttgactg ctctgttgga cgctcaacgg ttaatcaccc acttggtggt
     7741 cgttaccaac tcacaccaac tgaggcatct gtgcagaaat tgccagttca acacggtgtg
     7801 actcatactg cgtcggtcat tgctcaaggt ttcaacccat atgtagctga atggtctcca
     7861 taccacggtg ctgcttatgc ggttatcgaa gcaactgctc gtttggtggc tgctggtgcc
     7921 aactggtcta aggctcgttt ctcttaccaa gaatatttcg agcggatgga caagcaagca
     7981 gagcgtttcg gtcagccagt agctgctctt ctaggttcta ttgaagcaca aattcagctt
     8041 ggcttgccat ctatcggtgg taaggactcc atgtctggta cctttgaaga attgaccgtt
     8101 ccgccaacct tggttgcctt tggggtgacg acggcagata gccgtaaggt gctctctcca
     8161 gaatttaaag ctgttgggga aaatatctac tacatcccag gtcaagccct ctctgcagag
     8221 attgattttg acttgattaa gaaaaatttt gctcagtttg aagcaagcca agctgaccat
     8281 aaagtgacgt ctgcatcagc tgtcaaatac ggtggtgtag ttgaaagttt ggctcttgct
     8341 acctttggaa actatattgg tgcagaggtg accttgcctg aacttaaaac agctttgaca
     8401 gctcaattag gcggctttgt cttcacatct cctgaagaaa ttgctggagt agagaagatt
     8461 ggacaaacga aagcagactt tacactgact gtcaacggtg tgaagctaga tggacacaag
     8521 cttgacagtg catttcaagg gacattggaa gaagtttacc caacagaatt tacccaagcg
     8581 aaagaactag aagaagtacc agctgtggca tcagatgttg tgattaaagc caaagaaaag
     8641 gttgaaaaac ctgtggttta catcccagtc tttccaggaa ccaactcaga atatgattca
     8701 gctaaggcct tcgaaaaaga aggtgcagag gtcaatttgg tgccattcgt gaccttgaat
     8761 gaagaagcta ttgtcaagtc agttgaaact atggttgaca atatcgacaa gactaatatt
     8821 ctcttctttg ccggtggatt ctcggctgcg gatgaaccag atggttcagc taagtttatc
     8881 gtcaatatcc tgcttaatga aaaagtgcgt gtggctattg atagctttat cgcccgtggt
     8941 ggcttgatta tcggtatttg taatggattc caagccttag tcaaatcggg tctcctacca
     9001 tacggaaact ttgaagatgc taacagtact agtccaaccc tcttctacaa tgatgccaac
     9061 caacacgtga ccaagatggt ggaaactcgc attgccaata ccaactcacc atggttggct
     9121 ggtgtgcaag tgggcgatat ccacgctatt cctgtttcgc acggtgaagg gaagtttgtc
     9181 gtgacggctg aggaatttgc agagctccgt gacaatggac aaattttcag ccaatacgtt
     9241 gactttaacg gtaaaccaag tatggattct aagtacaatc cgaatggttc tgtccatgcc
     9301 atcgaaggaa ttaccagcaa gaatggtcaa atcatcggta agatgggcca ctcagaacgt
     9361 tatgaggatg gtcttttcca aaatatccca ggcaataaag accaacacct gttcgcatca
     9421 gcggttaaac atttcactgg aaaataagac ttacagattt tctaatagat agtatcagta
     9481 atgtaaaagt catgtaaatc tagctcttga tgattacaaa tgaaaattag gtataaaaaa
     9541 tgacatacga agtaaaatct cttaatgaag aatgtggtgt tttcggtatt tggggacatc
     9601 cagatgctgc taagttgacc tattttggac tccacagtct tcaacaccgt ggtcaggagg
     9661 gggcaggaat cctctccaat gatcaaggac aactgaagcg ccatcgtgac atggggcttt
     9721 tatcagaagt tttcagaaat ccagccaatt tggataaatt gacaggagct ggtgcgattg
     9781 ggcatgtgcg ttatgcgact gctggcgaag cttctgttga taacatccag cccttcctct
     9841 tccgttttca cgatatgcag tttggcttgg ctcataatgg aaatctgacc aatgcagcct
     9901 ctctcaagaa agaactggaa caaagaggag caattttcag cgcgacttcg gactctgaaa
     9961 tcttggctca cctcattcgt cgtagtcata atcctagcct gatgggcaaa atcaaggaag
    10021 cgctcagcct tgtcaaaggt ggttttgcct atatcttgct gtttgaggac aagttgattg
    10081 cggctcttga ccctaatggc tttcgtccgc tttctatcgg gaaaatggcc aacggagcgg
    10141 tggttgtttc ctctgaaacc tgtgcttttg aggtcattgg tgccgagtgg attcgtgatt
    10201 tgaagccagg tgagattgtg atcattgatg acgagggcat tcagtatgac agctatacag
    10261 atgatactca gttggcgatt tgttctatgg agtatatcta ctttgctcgc cctgattcta
    10321 atatccacgg tgtcaatgtc catacggcac gtaagagaat gggagcgcaa ttggcgcgag
    10381 aatttaagca tgaggcagat attgtagttg gtgtgcccaa ttcttcccta agcgcggcta
    10441 tgggatttgc ggaagaatca ggcttaccaa atgaaatggg tctgatcaaa aaccaataca
    10501 cccagcgaac ttttatccaa ccgactcaag aattgcggga gcaaggagtg cggatgaaac
    10561 tgtctgctgt ttcgggtgtt gtcaaaggca aacgtgtggt catggtggat gattccattg
    10621 tacgtggaac aacctctcgt cgtatcgttc agctcttgaa agaagcgggt gcgactgagg
    10681 ttcacgttgc cattggaagt cctgcactag cgtatccatg tttctacggg attgatatcc
    10741 agacccgtca ggagctgatt gcagccaatc atacggtcga agaaactcgc caaatcattg
    10801 gtgcggacag tctgacttat ctttcaattg atggcttgat tgagtcgatt ggtatcgaaa
    10861 cagatgcgcc gaacggtggt ctctgtgtcg cttactttga cggtgactac ccaacgcctc
    10921 tctatgacta cgaagaagac tatcgtagaa gtttggaaga aaagaccagt ttttacaagt
    10981 aggcgacaga ttctccatta aagaaaagga aaaaacaaat gacaaataaa aatgcatatg
    11041 cccagtcggg tgtggatgtt gaagcgggtt atgaagttgt tgaacggatt aaaaagcacg
    11101 tggcccgtac ggagcgtgca ggtgtcatgg gagctcttgg tggctttggt ggtatgtttg
    11161 acctttccaa gactggggtt aaagaacccg tcttgatttc agggactgac ggtgtcggaa
    11221 ccaagctcat gttggctatc aagtacgaca agcacgatac catcgggcag gactgtgtgg
    11281 ccatgtgtgt caacgacatc attgctgcag gtgcggaacc cctctatttt ctcgactacg
    11341 tagcgacagg gaagaatgaa ccagctaagc tagaacaagt ggttgctggt gtggcagaag
    11401 gttgtgtgca ggctggtgct gccctcatcg gtggggaaac ggctgaaatg ccgggcatgt
    11461 acggcgaaga cgactatgac ttggctggtt ttgcggtcgg tgtggctgaa aaatctcaaa
    11521 tcattgacgg ttcaaaggtg gtagagggag atgttcttct cggacttgct tcaagtggga
    11581 ttcactcaaa tggttactct ttggttcgtc gtgtctttgc ggattacaca ggtgaggaag
    11641 tcctaccaga attggaaggc aagaaactta aggaagttct acttgagccg actcgtatct
    11701 atgtcaaggc tgtcttgccg ctcatcaaag aagagttggt caacggcatt gcccacatca
    11761 caggtggtgg ctttatcgaa aatgtccctc gtatgtttgc agatgaccta gctgctgaaa
    11821 ttgatgaaag caaagttcca gtgcttccaa ttttcaaagc ccttgaaaaa tatggtcaga
    11881 ttaaacacga agaaatgttt gaaatcttca atatgggtgt gggacttatg ttggcggtca
    11941 gccctgaaaa tgtagagcgt gtaaaagaat tgttggatga agcagtctat gaaattggtc
    12001 gcatcgtcaa gaaagaaaac gaaagtgtca ttatcaaatg aaaaaaatag cggtttttgc
    12061 ctctggtaat ggctcaaatt ttcaggtgat tgccgaagaa tttccagtgg agtttgtctt
    12121 ttcagaccat cgtgatgcct atgtgcttga gcgtgcaaag cagctcggcg ttctgtccta
    12181 tgcttttgaa ctcaaggagt ttgagagcaa ggcagactac gaagcagccc ttgtcgaact
    12241 cttggaagaa caccagattg ccttggtttg cctagcaggc tacatgaaaa tcgttggacc
    12301 aaccttattg tcggcttatg aaggtcggat tgtcaacatt catccagcct acttgccaga
    12361 atttccagga gctcatggga ttgaggatgc ttggaatgct ggcgtgggtc agtctggtgt
    12421 gaccattcac tgggtggatt cgggtgtgga tacaggccag gtcatcaaac aggttcgtgt
    12481 gccacgacta gctgatgata ccattgacag atttgaagct cgcatccatg aagcagagta
    12541 caggttgtat ccggaagtag tgaaggctct atttacagat tgactttttg atgattcata
    12601 tgatatcttt gattttaaat tggagtcagt gtttgttgaa gacggcttca aacggaggta
    12661 tttgtaatgt tagaatctaa aaaaacaact cgatatgtat tttatgtcta tctgatgtta
    12721 ttaacttggg gaatcttatt taagtttgaa acaaatcctg aatttatagc atttttctta
    12781 gctccaaggt atatcaattg gattccattt tcagaaccac taatagtcga tggaaaaatt
    12841 gtttttgctg aaatgttatt taatctgatt ttctttattc cattaggtgt ttgtttccct
    12901 ttgataaaaa ctaatttatc tagtttaaga atagtcggga caggtttctt gattagttta
    12961 ttgtttgagt gcttacagta tattttagca ataggtataa cagatataac ggatttgact
    13021 ttaaatacgc taggtgtctg tgtaggctta ctgatttatc aaatttttat aagagtgttc
    13081 aaatcacaga ctagaaaatg gatcaatatc ttaggtatgc ttagccttgg ttttgcttat
    13141 cttgttttac tgttactgca tttaattggt gtttaactaa tgattaaaaa ggagaatata
    13201 atgactaaac gcgtcttaat cagcgtctca gacaaagcgg acattgttga atttgcccaa
    13261 gaactcaaaa aacttggttg ggagattatc tcgacaggtg gaactaaggt tgcccttgat
    13321 aatgctgggg tggataccat tgctatcgat gatgtgactg gtttcccaga aatgatggac
    13381 ggtcgtgtga agaccctcca cccaaatatc cacggagggc ttctcgctcg tcgtgacttg
    13441 gatagccact tggaagcggc taaggacaac aagattgagc tcattgacct tgtggtggtc
    13501 aacctttacc catttaagga aactatcctt aaaccagatg tgacttatga tgatgcagtt
    13561 gaaaatatcg atattggtgg gccatctatg cttcgttcag cagcgaaaaa tcatgccagt
    13621 gttacagttg tggtagatcc tgctgactac gctgtggttt tggacgaatt ggcagcaaac
    13681 ggcgaaacct cttatgaaac tcgccaacgt ttagcagcca aagtatttcg tcacacagcg
    13741 gcttatgacg ccttgattgc agaatacttc acagctcaag tgggtgaaaa caagcctgaa
    13801 aaactcactt tgacttatga cctcaagcaa ccaatgcgtt acggtgagaa tcctcaacaa
    13861 gacgcggact tttaccagaa agctttgcct acagactact ccattgcttc agccaaacag
    13921 ctcaacggga aagaattgtc atttaataat atccgtgatg cagatgctgc tatccgtatc
    13981 atccgtgact tcaaagatag tccaaccgtt gtggctctca aacacatgaa tccatgtgga
    14041 attggtcaag ctgatgacat cgagactgct tgggactacg cttatgagtc tgacccagtg
    14101 tctatctttg gtgggattgt cgtcctcaac cgtgaggtgg atgctgcgac agctgagaag
    14161 atgcacggcg ttttcctcga aatcatcatt gcaccaagct atacggatga agcgctagcc
    14221 attttgatca ataaaaagaa aaacttgcgt atccttgcct tgccatttaa tgctcaagag
    14281 gctagcgaag tggaagcaga atacacaggt gtagtcggtg gacttctcgt gcaaaatcaa
    14341 gacgtggtca aggaaagccc agctgactgg caagtggtga ctaaacgtca gccaactgag
    14401 acagaagcga ctgctcttga gttcgcttgg aaggctatca agtacgtcaa atcaaatggt
    14461 attatcgtga ccaacgacca catgacactt ggtgttggtc caggtcaaac caaccgtgtg
    14521 gcttctgttc gccttgccat tgaccaagcc aaagatcgtc tggacggggc ggtccttgct
    14581 tcagatgcct tcttcccatt tgcggataac gtggaagaaa tcgccaaagc aggaattaag
    14641 gccatcatcc agcctggtgg ctctgtccgt gaccaagaat ccatcgaagc agcggataaa
    14701 tacggcttga ctatggtctt tacaggtgtg agacatttta gacattaaga agataaaagg
    14761 gaagaaaaca gtttctttcc ttttttggct taaaatgcta actgaaacaa gattaaaacg
    14821 aacttttttg atataatgtt ggtaaataat tcgcaaaaga ggttgaggaa tgaaactgct
    14881 tgttgtcggt tctggtggtc gtgagcatgc gattgctaaa aagttacttg aatcaaaaga
    14941 cgtggaaaaa gtctttgtag ctcctgggaa tgatgggatg actctggatg gtttggaatt
    15001 ggtaaatatc tctatttccg aacattctaa attgattgac ttcgcaaaga ccaatgatgt
    15061 tgcttggacc tttatcggtc cagatgatgc ccttgctgct ggtatcgtgg atgattttaa
    15121 ccaagctggg cttaaggcct ttggtccgac tagggctgca gcggagctgg agtggtccaa
    15181 ggattttgcc aaggaaatca tggtcaaata tggtgtttcg acagcagcct atggcacatt
    15241 ttcagatttc gaggaagcaa aggcctatat cgaaaagcat ggtgcgccta tcgtagtcaa
    15301 ggcggatggc ttggcgcttg ggaagggtgt cgtcgtttct gagacggttg agcaagcggt
    15361 cgaagccgct catgagatgc ttttggacaa taaatttggt gactcaggtg cgcgcgtggt
    15421 tattgaggaa ttctttgaag gagaggaatt ttcactcttt gcctttgtca atggtgataa
    15481 gttctacatc atgccaacgg ctcaggacca caaacgtgcc tatgatggcg acaaagggcc
    15541 taacacgggt ggtatgggtg cctatgcgcc agttccacac ttaccacaga gtgtagttga
    15601 tacagcggtt gacaccattg tcaagccagt tctagaaggg atgattaaag aaggtcgccc
    15661 ttatctggga gttctttacg cagggcttat cctgacagct gatggaccga aagtcattga
    15721 gttcaacgct cggttcggag atccagaaac tcagattatc ttgcctcgct taacctctga
    15781 ctttgctcaa aatatcacag atatcctgga tagcaaggag ccaaatatca cgtggacgga
    15841 caagggtgtg actctgggtg tggttgtcgc atccaagggc tacccgctaa attattcaaa
    15901 gggtgttgag ttgccagtca aaaccgatgg cgacatcatt acctactatg caggggctaa
    15961 gtttgcggaa aatagcagag cactgctctc aaacggcgga cgagtttata tgctcgttac
    16021 cacagcagat accgtcaaag aagcccaagc cagcatctac caagaactat cccaacaaaa
    16081 aatagaagga ctcttctacc gaacagatat cggaagcaag gcaattaagt aaagatataa
    16141 gaataacgcg ccgtagtcgc caaacacgat aatggtcgtc gtggtgaaaa gaccagaaca
    16201 gtgaatgttc tggtcagggg gaaacttgga gaccgtaggc tcaaagttta ggaatgaaac
    16261 cgaaggtttg cttccgcctc catcacctaa gaccattatc aaaaagaaaa ataaaaattc
    16321 acaaaatacg ttaatgatcg tatggtttgc gagcgttagc gagctaatat agaacaatca
    16381 ccgccgttgt gaaagaacga ttggatgata atccaatcgt tcagggaaat tggaagacct
    16441 tgggtttcca atttaggcat gagacacctt tggtggctgc tgccgtccct cacaagctaa
    16501 ggtgattgtt gaaaaagagg aaaaaggaga agaaatgaaa ccagtaattt ccatcatcat
    16561 gggctcaaaa tccgactggg caaccatgca aaaaacagca gaagtcctag accgcttcgg
    16621 tgtagcctac gaaaagaaag ttgtttccgc acaccgcaca ccagacctca tgttcaaaca
    16681 tgcagaagaa gcccgtagtc gtggcatcaa gatcatcatc gcaggtgctg gtggcgcagc
    16741 gcatttgcca ggcatggtag ctgccaaaac aacccttcca gtcattggtg tgccagtcaa
    16801 gtctcgtgct cttagtggag tggattcact ctattctatc gttcagatgc cgggtggggt
    16861 gcctgttgcg accatggcta tcggtgaagc tggagcgact aacgcagctc tctttgccct
    16921 ccgtctcctc tctgtagaag ataagtccat tgcggatgca cttgccaact ttgctgaaga
    16981 acaaggaaaa atcgcagagg agtcgtcaaa tgagctcatc taaaacaatc ggaattatcg
    17041 gtggcggtca actgggtcag atgatggcca tttctgctat ctacatgggc cacaaggtta
    17101 tcgcgctgga tcctgcggcg gattgcccgg cctctcgcgt ggcggaaatc attgtggcac
    17161 cttataacga tgtggacgcc ctccgtcagt tggcagaccg ttgcgatgtc ctcacttatg
    17221 agtttgaaaa tgtcgacgct gacggtttgg atgccgttat caaggatgga caactccctc
    17281 aaggaacaga tctgctccgc atttcgcaaa atcgtatttt tgaaaaggac tttttgtcaa
    17341 acaaggctca agtcactgtg gcaccctaca aggtcgtgac ttctagccta gacttggcag
    17401 atatcgactt gtcgaaaaac tatgtcctca agactgcgac tggtggctac gatggtcatg
    17461 gacaaaaggt tattcgttca gaagcagact tggaagcagc ctatgcgcta gcagactcag
    17521 cagactgcgt cttggaagaa tttgtcaact ttgaccttga gatttctgtc atcgtgtcag
    17581 gaaatggcaa ggaggtgacg tttttcccag ttcaggaaaa tatccaccgc aacaatatcc
    17641 tgtctaagac catcgtacca gcccgcattt ctgaaagtct agcagacaag gctaaagcta
    17701 tggcagtgcg aatcgcagaa caactcaact tgtctggaac tctctgtgtg gaaatgtttg
    17761 cgactgatga tgacgttatt gtcaatgaaa tcgccccacg accacataac tctgggcact
    17821 attctattga agcctgtgat ttctctcagt ttgacaccca tattctgggt gttctcggag
    17881 caccattacc agtcatcaaa ctccatgcgc cagccgttat gcttaatgtc ctcggtcagc
    17941 atgtcgaggc tgctgagcaa tatgtgacag aaaattcaag cgcccacctc cacatgtatg
    18001 gtaaaataga agcgaagcac aaccgcaaga tgggacatgt gaccttgttt agtgatgtac
    18061 cggatagtgt ggatgagctt ggggaaggga ttgattttta ggacaagtct atgatacaaa
    18121 ttatcgttaa tacatttatt gaaaaggata agactggagc agttgttgaa gtgttgtatg
    18181 ccagtgctga ccaagataag gtacaagcta aatatgaaga actagctgca caataccccg
    18241 aaaattattt agctatctat gatgtaccgc tggatacgga tttgaataca ctagatcatt
    18301 acccgtctgt gtttattgaa aaagaggagt ttgagtagaa atcttggttt acctagatag
    18361 cttattccca acagcttaag aagaaaggaa aaattaacac atgatcaacc gttactctcg
    18421 ccctgagatg gcgaatattt ggagtgaaga aaataaatac cgtgcttggc ttgaggtgga
    18481 aatcctctct gacgaggcat gggctgagtt gggggaaatc cctaaggaag atgtggcttt
    18541 gattcgcaag aaggcggact ttgacatcga ccgtattttg gaaattgagc aggagacgcg
    18601 ccacgatgtg gtggctttca cgcgtgcggt ttctgagact cttggtgaag agcgcaagtg
    18661 ggttcactat gggttaactt ctaccgacgt ggtggatact gcttatggtt acctctacaa
    18721 gcaggccaac gacatcatcc gtcgtgacct tgaaaacttc actaatatca tcgctgacaa
    18781 ggccaaggag cacaagttca ccatcatgat ggggcgtact catggtgtgc acgctgagcc
    18841 gacaaccttt ggtcttaaat tggcaacttg gtacagcgaa atgaaacgca atatcgagcg
    18901 tttcgagcat gcagctgctg gtgtagaagc tggtaagatt tctggtgcgg ttgggaactt
    18961 tgccaatatc ccaccatttg tagaggagta tgtctgcgat aaacttggca tccgtgccca
    19021 agaaatctct acacaagtcc ttcctcgtga ccttcacgct gagtactttg cggttcttgc
    19081 cagcattgcg acttcaatcg aacgtatggc gactgagatt cgtggtctac aaaaatctga
    19141 gcaacgcgaa gtagaagagt tctttgctaa agggcaaaaa gggtcttcag caatgcctca
    19201 caaacgcaac ccaatcggtt ctgaaaatat gactggtctg gcgcgtgtca ttcgtggtca
    19261 catgattacg gcttatgaaa acgtcgctct ctggcacgaa cgcgatattt ctcactcatc
    19321 agctgagcgt atcatcacac cagatacgac cattttgatt gactacatgc tcaaccgttt
    19381 tggaaatatc gtcaagaact tgacagtctt cccagaaaat atgatccgaa acatgaactc
    19441 gacttttggt cttatcttta gccaacgggc tatgttgaca ttgattgaaa aaggcatgac
    19501 ccgtgagcaa gcctatgact tggtgcaacc aaaaacagcc tactcttggg acaaccaagt
    19561 agactttaaa ccacttcttg aggcagattc agaagtaaca tcacgtctca ctcaagaaga
    19621 aatcgatgaa atcttcaacc cagtttatta caccaaacga gtggatgata tctttgaacg
    19681 tcttggacta ggtgattaat taaaaaataa acagcgagct tcaatctcgc tgtttatttt
    19741 ttatcgaaaa gacttagtct tcttttcttt tagtgagtcc ataggctgct agtgtggcca
    19801 tgagtcctgc gactactagt cctgcagaat cgtgagttcc tgtttcagga agttttttct
    19861 ctattaccac aggagctgga tcttgaggaa gagctttgct ttcctcagca ggagcagttg
    19921 atggagctgg ttggcttggg atttctagtt ttggtttttc ttcagcaata gcggcttgtc
    19981 cgttttcatc gcctacatgt gttaccatag ttccgacttc gactatttga gtaacggctt
    20041 cctgtgctac gacactattt acaagtgttt tcacttcctt accatcggca gaagtgctca
    20101 cagagtagaa gttgctacga tgtccattga cgcccttagt aatgacttgt gtttttcctt
    20161 tgagtaagag tggattttca cgagtcactg tggtaaatgg aatttcttct ttttggatat
    20221 ccagtctagg ttttacctca gtagttggtg caagaccact ttcatcaccc ttgtgagtta
    20281 caggagcgcc aacttcaacc acttggttta taacttcttt ggttacctgg ctatcaagga
    20341 ctgtttctgt tgtttttcca ttttcagtga gtacagagat gtaatgagtt cgctcacctt
    20401 tgactcctgc tgtgataata ttttcctgac cagctgggag gttaggattt tctttcttga
    20461 taacttcaaa tggaatttct tcagttcttg tgatgagttc tggtctggtt tcaacattgg
    20521 cagccacttc attttcatcc aggcttcctg aatgagttgc agctggtttg aggccttgaa
    20581 gagcggcttt taggttggct acaagtgtgt caagctcagc ttgtttatta cggttgaggt
    20641 tgtaatttag agctgtttta gctgcgtcaa gggcctcaag actttcttta ctatatcctt
    20701 ctaagtttgt aggaatttta gctaattctt cgcggagagc attataatta gcacggaagt
    20761 agtctttgtt gtggtctgca aaggcagtca tgagttcaaa gatttcctct tccttgtatt
    20821 cagcgcttgg tctatctgcc cagattgaaa gcatacttcc gactgttgga agatctactt
    20881 caggatattt ggtagaagct agttgattga atggtgtttt tccagtattc tcaatagctt
    20941 tcttgaggaa accaccacca tcttctggtt tttgaccaag aatgtagtac cagtcaccgt
    21001 tggtattcaa gaatttatag cctttgcttg ctaggtattg aggtgatgcg aggttatatc
    21061 cccaccagcc tttagaccag taagaaatca agacatcttt gtcaaactga acatcgtcct
    21121 tgtcttcata gtagaagcca tcgttgaagg ccattggttg aagccctctt tctttggcca
    21181 tagctgcgag ggtgttggca tattcggcaa atttgccata gagttgatac cacttgaggt
    21241 agtaccagcc ttgggcacta gtcgcatcgt tggcgtattc gtcagtacca aagttgaaaa
    21301 tctttgtttt acctgcaaag aagtccatgt atttaccgat gagggctttt acaaagttca
    21361 tcgcttcttc gtttttcaag tccatagttg tttttgaaac tttatcaaag tgggcttgag
    21421 gatttttaat acctaatttt tccatggcaa ccagcatagc atccatgtga cctggactgt
    21481 taatagctgg gatgagaccg atgtccttag atttagcgta ttcaattagc tctgttactt
    21541 ctgcctgtgt tagtgcagta ccgtttggat cgtcgtagta agctttagtt ccttcgataa
    21601 tagctttttt aacgtcatca ctagcatagg tttttccgtt ggcagtaatg gtcatatcat
    21661 cgagtagaaa gcgaagtccg tcatttccta gaaggagatg gacatcagaa tatccgagct
    21721 cactggcctt gtctacgatg cgtttgagct ggttcagagt aaagtatttg cgtccagcat
    21781 cgattgagat taccttgttt ttggcaagtt tttcaacctc acgtttagct tcttcttctt
    21841 tttgagcttc aggcgtgagg gtcaagttgt tgacagtttc ttgaagttta gcaatggctt
    21901 gatcaatcgt atcttgttgg gcacggctaa ggttgctatc gagagagcga atagcttttt
    21961 cagcttcttt tacggccgcg acgctttctg cagtataacg gttcaggtct tttggtacct
    22021 cgttaagtgt ttgctctgca gattcataat cagctgcgaa gtattcagcg ttggcatttg
    22081 caaaatgacg catgagtttg aagaggcgtg atggtgaata acgtgcagat ggagtgtcag
    22141 cccaagcagc taccatacta ccgatgattg ggatatcagc tccttctgtt tttggtacag
    22201 aagtgattgg tgtgttttta ataccattga gcccctgatc gagattgtac cagccttggc
    22261 catcagcgtt tcgtccaaga acgtagtacc aagcatcatt ggtattaagg atttggtgac
    22321 ctttttcagc tagtagttta gaagaagcga catcgtagcc tccccaacca ccagtccaca
    22381 tagaaacgat gatgtctttg tcaaaactac caaagcttgt gtcgctattg tagtagatac
    22441 cgtcgttaaa ggccattggt ttgagaccgt gcgattttac aatacgagcg aggtcattgg
    22501 cgtaggcaat aaatttttca tagcctttta cagggtagcc ttcgtttgga tagtatttat
    22561 cagcttgaag cacactccaa cctttagcat ctgtcgcatc attggcatat tcatcaagtc
    22621 cgatgttgaa gatttcagtc tttttcgcga aataagcagc atacttgtcg ataagggctt
    22681 ttgtaaaagc gacagcttgt tcgttgtcaa gattgacagt acgggctgat tccttcccaa
    22741 aatagctaaa gttagggttt tggattccca attctttcat ggcattgaga atcgcatcca
    22801 tgtgaccagg actatttact gtcggaatga gaccgatacc tttatctttg gcatagttaa
    22861 tcagatctgt catttgactt tctgttaagt gattgccgtt tggatcgttg taataatcat
    22921 ttgtaccttt ttcaatggcg cgtttgacat cgtcactggc ataggtcttg ccgttagctg
    22981 tgatgctcat atcgtccaac atgaaacgga gtccatcatt tccgactaat aggtgtaaat
    23041 cagtgtagcc ataatgtttc gctttatcga tgatttcctt gagctgttct ggtgagaaat
    23101 atttacgtcc agcatcaata gaaacaattt tctttttcgc tagtttttca tttacagttg
    23161 cagcacgttc ctttcctgcc tctgtttccg gtttgtcatc ctctgctttc gcttcatctt
    23221 ttttagctgg tttatccttg tcagtcttgt ctgtatttga ctctttagaa tcaacctctt
    23281 tcgcttcttc ctttttaggg ctagcttctt ctgccttttt attagcagtt tctttttcag
    23341 cagaagttgg agttaccact tctgctttat cactaggagt tgaactaact tcctcttgtg
    23401 gtttttcttc tgtttttgga agactagcta ccttatcagt agctggagtt tctgtttcta
    23461 cagtttttgg agcttctggt tgaagctctg ctttaggtgt ttcctcagtc cgattttcgg
    23521 atgattgagg ggaatcagaa accgtatgga tggtcggttg gttttctgta gtagtaggag
    23581 taactccatc ggctgcaaca gtctgtgctt ggaaggcaaa tccaattaga acagaagctg
    23641 ctcctacagc gtatttacga atagaaaaac gctgttgttt ttcatgtttc attgcaaaac
    23701 ctcctgattg cattgttata ttgatagcga ttatataaat caacgccttt attttatttc
    23761 ttatattaac gagagtcaag aggagatgac aaaaaactat aataagtata aaaaaatata
    23821 aaatttaaac ttaagatttc agattggtcg gaaaaaatac gtatatatat ctagtataat
    23881 ttttggttct atttctataa aatattccac aaattataga attttccaaa aataggtaag
    23941 cgctaccttt ttggtgtggt ataataagca tagaaaaagc ccaagcgatt agctcaggct
    24001 ttcttcttag tgatcacggt cacatgagat aaatttaatc ttgtagtaat cagatcgttt
    24061 gtaagtttca ctgtattcta aaacttggcc agttgattcg agtttggtga ttttagtttg
    24121 taggacagta gggaattgtt catcgactcc gaggactgaa gccgcatgtt ctggagttgg
    24181 aaagactatt tcgttgattt cttcaaagtg ttcatcattc atgtgaatgt ggtagtctaa
    24241 cttgaaacga ttatagatag aactatagta ttcaaggttt ggataatttg cgttgatata
    24301 ttgttctggg atgtaggatg tatggtagat ataaacgaca ccgtttgatt cgcggatacg
    24361 ttcaatcttg tagtagaatt gatcgccgcg tagacccaat ttttccaagt aaacaagctt
    24421 gtttccgcgt tcaattgaaa gaacagttac cttatcatct ttagcattga agagttcaat
    24481 atctgaaaac tctacaagct tgtgtttgcg tgcacgtgaa acgaaggttc cttttccttg
    24541 ttggcggaca atatagccat ctttggcaag gtcgtttaag gcgcgaacaa ctgtgataga
    24601 gctgacatcg tacattgaaa tgagttctgc ttcagtgtaa aatttatctc cactgctaaa
    24661 ctgcccagag atgattttat tttttaattc gtcttttatg tattgatatt tgggaattgc
    24721 catattttta cctctatttt ccttctccat aggaaatttt accataaaag cgaaaaaaat
    24781 agtagtcttt tgaataaaaa attagaataa tagtttgaaa aattaagcga tatagggatt
    24841 gctcatattt tatcaatgta ttctatacaa aattatttta ggtcccatat tccgaattta
    24901 aagagcgttt gaaatgggtc taaaaatgtt tgaaactaat tgaatgtgtg tataatagta
    24961 aaaatgcagt aaatatcccg acaatttcta ttgacaattc aaacagattg gtttataatt
    25021 aatataacaa caaatgaaag cgcaaacttt cgcggtcgga aggtagtttt atgacacgat
    25081 ttgagatacg agatgatttc tatctcgatg gaaaatcatt taagatttta tctggtgcca
    25141 ttcattattt tagggttcct ccagaggatt ggtatcattc gctctataac ttgaaggctc
    25201 ttggttttaa tacggtagag acttatgttg cttggaattt acacgagcct cgtgaaggtg
    25261 agtttcattt tgaaggtgat ctggatttag agaaatttct ccaaatagcg caggatttgg
    25321 gtctctacgc aattgtgcgt ccgtctccat ttatctgtgc ggaatgggaa ttcggtggct
    25381 taccagcttg gctcttgacc aagaacatgc gaattcgctc atccgaccca gtatatatcg
    25441 aggcagttgg tcgctactat gatcagttat tgccaagact ggtgcctcgt ttgttggaca
    25501 atggtggcaa tattctcatg atgcaggttg aaaatgagta tggttcttac ggagaagata
    25561 aggcttacct gagagcgatt cgacagctaa tggaagagtg tggcgtaacc tgtcccctct
    25621 ttacatcaga tggtccatgg cgagctactc tgaaagctgg aaccttaatt gaagaggacc
    25681 tctttgtaac aggaaacttt ggttctaagg caccttacaa cttttcgcag atgcaggaat
    25741 tctttgatga acatggtaag aaatggccac tcatgtgtat ggagttctgg gatggttggt
    25801 tcaatcgctg gaaagaaccg attatcacac gggatcctaa ggaattggca gatgcagttc
    25861 gagaggtttt ggaacaaggc tctatcaatc tttacatgtt ccacggtggt acaaactttg
    25921 gtttcatgaa tggttgctca gctcgaggaa ctttggacct gccacaagtt acgtcttatg
    25981 attacgatgc ccttctggat gaagaaggaa atccaactgc taaatatctt gcagtcaaga
    26041 agatgatggc aacacatttt tcagagtatc cgcagttgga accactctac aaagagagta
    26101 tggagttgga tgctattcca ctagttgaaa aagtttcttt gtttgaaacc ttagatagct
    26161 tgtcaagtcc tgtagaaagt ctctatcctc aaaagatgga ggagctggga caaagttatg
    26221 gctacctact ttatcgaaca gaaacaaact gggatgcaga agaagaaaga cttcgtatca
    26281 ttgatggtcg agatagggcc cagctgtatg tcgatggtca gtgggttaaa actcaatatc
    26341 agacagagat tggggaagat attttttatc aaggtaaaaa gaaagggcta tctaggttag
    26401 atatcttgat agaaaatatg gggcgtgtca actatgggca taagttctta gcggatacgc
    26461 aacgtaaggg aattcggaca ggggtctgta aggatctgca tttcttacta aactggaaac
    26521 actatccact cccactagac aatcctgaga aaattgattt ttcaaaagga tggactcaag
    26581 gacaaccagc cttttacgct tatgacttta cagtcgaaga gccaaaagat acttacctag
    26641 acttgtctga gtttggtaag ggggttgcct ttgtcaatgg gcagaatcta ggacgttttt
    26701 ggaacgttgg cccaactctc tcactttata tccctcatag ctatctcaag gaaggtgcca
    26761 accgcatcat tatctttgaa acagaaggtc aatataaaga agagattcat ttaactcgta
    26821 aacctacact aaaacatata aagggggaaa acttatgaca attgtaggat gccgtattga
    26881 tggacgtttg atccacggac aagtagccaa tctttgggct ggaaaactaa atgtttcacg
    26941 cattatggtt gtagacgacg aagttgtcaa caacgatatt gaaaagagtg gtttgaaact
    27001 tgcgacacca ccaggtgtga aattgagtat tttgccagtt gagaaagctg cagccaatat
    27061 tcttgctggt aaatacgata gccaacgtct ctttatcgtg gctcgtaaac cagaccgctt
    27121 ccttggtttg gtagaagcag gtgtaccact tgaaaccctt aatgttggga atatgtctca
    27181 aacaccagaa acacgttcta ttacacgttc tatcaacgta gtagacaagg atgtggaaga
    27241 cttccacaaa ctggcagaaa aaggtgttaa acttactgct cagatggttc caaatgatcc
    27301 aatttcagac tttttgagct tattaaaata ggaaaaaaat ttttaggagg tcattgttat
    27361 gatacaatgg tggcaaattt tacttctcac tttgtactca gcttatcaaa tctgtgatga
    27421 gttgacgatc gtttcatctg caggttctcc tgtatttgct ggtttcatta ctggtttaat
    27481 catgggagat gtgactactg gtttacttat cggtggtaac ttgcaactgt tcgttcttgg
    27541 ggttggtacc ttcggtggtg cttctcgtat cgacgcaact tctggtgcgg ttcttgcgac
    27601 agccttctct gtttcacaag gaattgatgc accgcttgcc attactacaa tcgctgtacc
    27661 agtagcagct ctcttgactt acttcgacgt tcttggtcgt atgactacta ccttcttcgc
    27721 tcaccgtgtg gatgctgcaa tcgaacgctt tgactataaa ggtattgaac gcaactactt
    27781 gcttggtgcg attccgtggg ctctatctcg tgcccttcca gtcttctttg cccttgcttt
    27841 tggtggtgcc tttgtacaat cagtagtaga cttcgttgaa gcctacaaat gggttgcaga
    27901 tggcttgaca ctcgcaggac gtatgcttcc aggtcttgga tttgcaatct tgcttcgtta
    27961 ccttccagtt aaacgtaacc ttcactacct tgctatggga tttggtttga cagctatgtt
    28021 gactgttctt tactcatatg taacaggtct tggtggcgct gttgctggta tcgtaggtac
    28081 tcttcctgct gaagttgctg aaaaaattgg tttcgtgaac aacttcaaag gtttgtctat
    28141 gattggtatt tctatcgtag gtattttcct tgcagtgctt cacttcaaaa atagccaaaa
    28201 agtagctgta gcagcacctt ctacaccatc agaaagtggg gaaatcgaag atgacgaatt
    28261 ctaattacaa acttacaaaa gaagatttta atcaaatcaa caaacgtagc ttgtttactt
    28321 tccaattagg ttggaactac gaacgtatgc aagcttctgg ttacctttac atgatcttgc
    28381 ctcagttgcg taaaatgtat ggtgatggaa ctcctgaatt gaaagaaatg atgaaagttc
    28441 atactcaatt cttcaatact tcaccattct tccataccat tatcgctggt tttgaccttg
    28501 ccatggaaga aaaagatggt gtaggttcaa aagacgctgt taacggtatc aagacaggtt
    28561 tgatgggacc attcgctcct cttggggata caatctttgg ttcacttgta cctgctatca
    28621 tggggtcagt cgcagcaact atggctatcg ctggccaacc ttgggggatc ttcctttgga
    28681 ttgcagttgc agtagcgtat gacatcttcc gttggaaaca gttggaattt gcttacaaag
    28741 aaggggttaa ccttatcaac aacatgcaaa gtaccttgac agctttgatt gacgctgcat
    28801 ctgtacttgg tgtcttcatg atgggtgctc ttgtagcaac agtgattaac tttgaaattt
    28861 cttacaagtt gccaatcggt gaaaagatga ttgatttcca agacatcttg aaccaaatct
    28921 tcccacgttt gcttccagca atctttactg cctttatctt ctggttgctt ggtaaaaaag
    28981 gtatgaactc tactaaagct atcggtatta ttatcgtact tgctttggct ctttctgccc
    29041 ttggtcactt tgcacttgga atgtaattcc ttatgactaa atcattaatt ttggtgagcc
    29101 atggtcgctt ctgtgaggag cttagaggta gcacagaaat gattatgggc ccacaagaca
    29161 acatttacac agtagctctt cttccagaag atggcccaga agaatttact gctaaatttg
    29221 aagctgttat tgaaggattg gatgatttcc tagtctttgc ggatcttctc ggtgggacac
    29281 cttgtaatgt ggtgagtcgc ttgatcatgg aaggtcgtga tattgacctt tacgcaggga
    29341 tgaatcttcc aatggtgatt gaatttatca atgcgagcct tacaggcgca gatgcggact
    29401 acaagagccg tgctgcagaa agcattgtga aagttaatga cctgttagcg ggcttcgatg
    29461 atgacgaaga tgaataatac tcttcgaaaa tctcttcaaa ctacgtcaac gtc
//
LOCUS       DAVBHS010000009        28412 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVBHS010000009 DAVBHS010000000
VERSION     DAVBHS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28412
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.009"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            46..534
                     /locus_tag="VQ651_000264"
     CDS             46..534
                     /locus_tag="VQ651_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HET2753313.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            531..1718
                     /locus_tag="VQ651_000265"
     CDS             531..1718
                     /locus_tag="VQ651_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2753314.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            1711..2076
                     /locus_tag="VQ651_000266"
     CDS             1711..2076
                     /locus_tag="VQ651_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2753315.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            2092..3438
                     /gene="asnS"
                     /locus_tag="VQ651_000267"
     CDS             2092..3438
                     /gene="asnS"
                     /locus_tag="VQ651_000267"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET2753316.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            3591..3881
                     /locus_tag="VQ651_000268"
     CDS             3591..3881
                     /locus_tag="VQ651_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET2753317.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            3893..4363
                     /gene="ssbA"
                     /locus_tag="VQ651_000269"
     CDS             3893..4363
                     /gene="ssbA"
                     /locus_tag="VQ651_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HET2753318.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            4395..4634
                     /locus_tag="VQ651_000270"
     CDS             4395..4634
                     /locus_tag="VQ651_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET2753319.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            4929..6329
                     /locus_tag="VQ651_000271"
     CDS             4929..6329
                     /locus_tag="VQ651_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HET2753320.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSYVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDVIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            6331..6690
                     /locus_tag="VQ651_000272"
     CDS             6331..6690
                     /locus_tag="VQ651_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET2753321.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            6744..7493
                     /locus_tag="VQ651_000273"
     CDS             6744..7493
                     /locus_tag="VQ651_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HET2753322.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            7483..7782
                     /locus_tag="VQ651_000274"
     CDS             7483..7782
                     /locus_tag="VQ651_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET2753323.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALFKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            7853..8788
                     /locus_tag="VQ651_000275"
     CDS             7853..8788
                     /locus_tag="VQ651_000275"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HET2753324.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            8875..9387
                     /locus_tag="VQ651_000276"
     CDS             8875..9387
                     /locus_tag="VQ651_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET2753325.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGAVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            9424..10019
                     /locus_tag="VQ651_000277"
                     /pseudo
     CDS             9424..10019
                     /locus_tag="VQ651_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            10120..10314
                     /locus_tag="VQ651_000278"
     CDS             10120..10314
                     /locus_tag="VQ651_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET2753326.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(10360..11805)
                     /locus_tag="VQ651_000279"
     CDS             complement(10360..11805)
                     /locus_tag="VQ651_000279"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HET2753327.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            11911..13533
                     /locus_tag="VQ651_000280"
     CDS             11911..13533
                     /locus_tag="VQ651_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET2753328.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            13737..15695
                     /locus_tag="VQ651_000281"
     CDS             13737..15695
                     /locus_tag="VQ651_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2753329.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGFLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFATAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            15867..16802
                     /locus_tag="VQ651_000282"
     CDS             15867..16802
                     /locus_tag="VQ651_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2753330.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDTSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILLLSNISEKYSRRLQEEIGFLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERV
                     QNNSLKSDRVTSFETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLL
                     NPISNIANFVTVYSRSKGSSVALENLLAVPKEKFE"
     gene            16827..17479
                     /locus_tag="VQ651_000283"
                     /pseudo
     CDS             16827..17479
                     /locus_tag="VQ651_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            17637..18731
                     /locus_tag="VQ651_000284"
     CDS             17637..18731
                     /locus_tag="VQ651_000284"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET2753331.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            18739..19905
                     /locus_tag="VQ651_000285"
     CDS             18739..19905
                     /locus_tag="VQ651_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2753332.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEIQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            19998..23096
                     /locus_tag="VQ651_000286"
     CDS             19998..23096
                     /locus_tag="VQ651_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2753333.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDSFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLNRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            23144..23761
                     /locus_tag="VQ651_000287"
     CDS             23144..23761
                     /locus_tag="VQ651_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET2753334.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            23771..25105
                     /locus_tag="VQ651_000288"
     CDS             23771..25105
                     /locus_tag="VQ651_000288"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET2753335.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            25115..25579
                     /locus_tag="VQ651_000289"
     CDS             25115..25579
                     /locus_tag="VQ651_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2753336.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     DLDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            25576..26070
                     /locus_tag="VQ651_000290"
     CDS             25576..26070
                     /locus_tag="VQ651_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2753337.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            26151..27800
                     /gene="mltG"
                     /locus_tag="VQ651_000291"
     CDS             26151..27800
                     /gene="mltG"
                     /locus_tag="VQ651_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET2753338.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMTDSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDIIS
                     DTSRRSRREGAKPVNPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPIDA
                     NSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQKSM
                     STEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAEAFLA
                     KVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDEML
                     AAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDMPL
                     QSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIESSI
                     NQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            27871..28353
                     /gene="greA"
                     /locus_tag="VQ651_000292"
     CDS             27871..28353
                     /gene="greA"
                     /locus_tag="VQ651_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET2753339.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
BASE COUNT         8523 a         5294 c         6405 g         8190 t
ORIGIN      
        1 ttatattcac ttgtaataaa gcttaataga tggtaaaata gacgagtgaa aaaaagacaa
       61 aacaaagcaa aaaataatct actgtggcag tatggtctag ggatgacgat tttgtttgtg
      121 gttatcagtg cttcctttct gtatatggtt tctcttagca tgaaacccta tcaaacagct
      181 aaaagtgaag gagaaaaatt agctcagcag tatgcaggat tagagcaggc cgatcaggtt
      241 gatttataca atggcttgga atcttattac agcgttcttg gtcgtaataa acagcaagaa
      301 gcacttgctg ttctgattgg aaaagatgat cataagattt acgtttatca gctaaatcag
      361 ggtgtttcac aagaaaaagc agaaacggtt tctaaggaaa agggagctgg cgaaattgac
      421 aagattatct ttggtcgtta tcaagataag ccaatctggg aagtcaagtc aggatctgat
      481 ttttatctag tagattttga aacaggagca ttggtcaaca aggagggcct atgaaattat
      541 ccaaccgtgt tttagaaatg gaagaaagtg tgactttggc tgctggagcc cgtgccaaag
      601 cactgaaggc tgagggtaga gatattttgt ctctaacctt gggtgagcca gatttcacta
      661 ctcccaaaaa tatccaagat gccgccattg catcgattcg agatgggcgt gcttcttttt
      721 atacagtaac ctcaggtctg ccagagctta aggcggcggt caatagctac tttgagcgct
      781 tttacggcta ttctgtagcg tcaaatcaag tgacagtcgc tgcgggagcc aaatattctc
      841 tctatacctt ctttatggct gtggtcaatc caggtgatga agtcatcatc ccaaccccat
      901 actgggttag ctatggagat caggtcaaga tggcagaagg cgttcccgtc tttgtttctg
      961 ctaaggaaga caatcacttt aaggtgaccg tagagcagtt agaagcagct cgcactgaca
     1021 agaccaaggt tttggtgctg aattcgccat ctaatcccac aggtatgatt tacacccgtg
     1081 aggaactctt ggcaattgga aactgggctg tagaaaatga tattctcatc ctagcagatg
     1141 atatctatgg ccgcttggtt tataatggtc atgagttcac accgatttct agcctatcgg
     1201 aagcgattcg caagcaaaca gtggtcatca atggtgtgtc taaaacctat gccatgactg
     1261 gttggcggat tggttatgcc gttggagaag cagacattat cgctgccatg tccaagattg
     1321 caggtcaaac aacttcgaat ccgtcagcag tagcccaata tgcagcagtt gaggctctat
     1381 caggtgagca agatactgta gaaagcatgc gtcaggcctt tgaagaacgt cttaatacca
     1441 tctatcccct ccttgcagaa gtgccaggat ttgaagtggt caaaccgcaa ggggccttct
     1501 atctcttccc aaatgtcaaa aaggccatgg agatgaaagg ctacacggat gtgacagact
     1561 ttacaactgt tatcttagaa gaagccgaag tggccttggt aacaggagct ggatttggag
     1621 caccagaaaa tgtccgcctc agctatgcga cagacctaga cacgcttaaa gaagcagtcg
     1681 aacgcttgaa agcatttatg ggtagtgaga atgattgatc attttgagat taaggtaaag
     1741 gatttacaaa tttcagaagg attttatagg agttttctcg ctcctttgga ctataaattg
     1801 gcttttaaga ctagttctct aattagtttt ctatccccga acagccctca tcctggtggt
     1861 gatttttggc tgactcaggg aacacaagat cctgttcatt ttgctttttt agcagaaaat
     1921 aaggaagaag ttcaggcttg ttatgaggct ggcttagagg caggtgggcg agacaatggg
     1981 gctcctggtt atcgaagtga gcatccgatt tactatgctg cttttatgat tgacctggat
     2041 gggaacaata tagaggtggt ttgccataaa gaataaaaaa gaaaaggaaa aataatgaca
     2101 aaacgtgtaa cgattattga cgtaaaagac tatgttggtc aggaagtgac gattggcgct
     2161 tgggttgcca acaaatcagg aaaagggaaa atcgctttct tacaattgcg tgatggaaca
     2221 gccttctttc aaggtgtggc ttttaaacca aactttgtcg aaaaatttgg tgaagaagtg
     2281 ggacttgaga agtttgatgt tatcaaacgc ttgagccaag aaacgtctgt ttatgtgaca
     2341 ggtattgtca aagaggacga acgttctaaa tttggctatg agttggacat cacagacatc
     2401 gaagtgatcg gtgaatctca agactaccca atcacaccaa aagaacacgg aacagacttt
     2461 ttgatggata accgtcactt gtggctacgc tctcgtaagc aagtagctgt gttgcaaatc
     2521 cgtaacgcta ttatctatgc aacttatgag ttctttgaca agaacggttt tatgaagttt
     2581 gacagcccaa ttctttcagg aaatgcggca gaagattcta cagaactctt tgaaactgac
     2641 tacttcggaa cgccagccta cttgagccaa tcaggtcaac tttacctaga agcaggggct
     2701 atggctcttg gtcgtgtctt tgactttggt ccagttttcc gtgctgaaaa atcaaaaaca
     2761 cgccgtcact tgactgagtt ctggatgatg gatgctgagt actcatactt gacacatgat
     2821 gagtcgcttg acttgcaaga agcttatgtg aaagctcttc tacaaggtgt tcttgaccgc
     2881 gcgcctcaag ccttggaaac cttggaacgt gatacagaac tcttgaaacg ctacattgca
     2941 gagccattca aacgtatcac ttacgatcaa gccattgacc tcttgcaaga gcatgaaaat
     3001 gatgaagatg ctgactacga gcatcttgag catggtgatg actttgggtc accacacgaa
     3061 acttggattt caaaccactt tggtgtgcca acatttgtca tgaactatcc agcagccatc
     3121 aaggccttct acatgaaacc agttcctgga aatccagagc gcgtgctttg tgcagacttg
     3181 cttgctccag aaggctatgg agaaattatc ggtgggtcta tgcgtgagga agattacgat
     3241 gcccttgtcg ctaagatgga tgaacttggc atggatcgta cagaatatga attctacctt
     3301 gaccttcgta aatacggtac agttccacac ggaggatttg gtatcggtat cgaacgtatg
     3361 gtaaccttcg cagcaggaac aaaacatatc cgtgaagcta ttccattccc acgtatgttg
     3421 caccgtatca aaccataaga acaggaaaac gcccatgtgg cgttttttcg aattctagtg
     3481 tttttacttg cgtaaaaaaa atttttctag tataatagtt tattgtgagc gaacctcact
     3541 taccccttgc aaagtcttgg ggtcattaga ccaaaaggag gaacatatca atggctaaat
     3601 acgaaattct ttatatcatt cgtccaaaca ttgaagaaga agctaaaaac gctttggtag
     3661 cacgttttga ctctattttg actgacaacg gtgcaactgt tgttgaatca aaaacttggg
     3721 aaaaacgtcg tcttgcatac gaaatccaag atttccgtga aggactttac cacatcgtta
     3781 acgttgaagc aaatgacgat gcagctctta aagagtttga ccgtctttca aaaatcaacg
     3841 ctgacattct tcgtcacatg atcgtcaaaa ttgacgcgta agaaggtaaa ctatgattaa
     3901 caatgttgta cttgtagggc gtatgacacg tgacgctgaa ttgcgttata ccccatcaaa
     3961 tgtagcagtt gcgactttta ctcttgcagt aaaccgtaca tttaagagtc aaaatggtga
     4021 acgtgaggct gattttatca atgtcgttat gtggcgccaa caggctgaaa atcttgctaa
     4081 ctgggctaaa aaaggctcac ttatcggggt gacaggtcgt atccagactc gtagttacga
     4141 taaccagcaa ggacaacgtg tctacgtgac agaggtcgtg gctgagaatt tccaaatgtt
     4201 ggaaagccgt agtgtgcgtg agggtcacac aggtggagct tactctgcac caactgcaaa
     4261 ctattcagca cctacaaatt cagtaccaga cttttcacgt aatgaaaatc catttggagc
     4321 aacaaaccca ttggatattt cagatgatga tttaccattc taatggacaa ctaaacttat
     4381 aaaggagaaa aaacatggct caacaacgtc gtggcggatt caaacgccgt aaaaaagttg
     4441 attacatcgc agcaaacaaa attgaatatg ttgattacaa agatactgag cttcttagcc
     4501 gtttcgtttc agaacgtggg aaaatccttc ctcgtcgtgt aacaggaact tcagctaaaa
     4561 accaacgtaa agtaacaaca gctatcaaac gcgctcgcgt aatggctttg atgcctttcg
     4621 taaacgaaga ttaaagaaaa gacccggttg ggtctctttt tcatgaactt gaaaacaaga
     4681 aagcgaatta agaccccaac gggtcttttt tcacaagctt tgaaatgaga gagcgagtta
     4741 ggctcagtgg ctcccttttt acgagtttga aaacaagaga acacattaaa agcacgatgg
     4801 agctttttca taaatatgtc ttttttttca tccaagaaat taaaataaaa atgataaact
     4861 ttttgaaaat aggggatttg cctactttat gatataatgg aaagagttag attgaaagag
     4921 gtagtgagat ggacgcgaaa ttaagataca aggcaaagaa gatcaagatt gtcttttttg
     4981 atattgatga tacattgcgg aattcaaaga cagggtttat tccaactaca attcccactg
     5041 tttttaaaca gttgcgtgaa aaaggaattt taacaggaat cgcctctgga cgtggcattt
     5101 ttggtgttgt tccagagatt cgtgatctca aacctgactt ttttgtaact ttgaatgggg
     5161 cttatatcga agataaaaaa ggtcaggtca tttatcagca tcagattgaa aagtcatatg
     5221 ttgaggagta tatctcttgg gctaagcaag aaggaattga gtatggcttg gttgggagtc
     5281 atgatgccaa gttgtcgact cgcaccgata tgatgagtga agctatcaat ccaatttatc
     5341 ccgacttaga tgtagatccc gatttccatg aaaaagaaga tatctatcag atgtggactt
     5401 ttgaagataa gggagatgac ttgcacttgc ctgacagtct ctcagacaaa cttcgcatgg
     5461 ttcgttggca tcaacattcg tctgatattg tgccgatttc aggctccaaa gcgacggggg
     5521 tggaaaaggt tgtggaacac cttggcttga aaccagagaa ggtcatggtt tttggagatg
     5581 gtctcaatga cttggaactc tttgattatg ctggaatcag cgttgccatg ggaatttctc
     5641 atgataaaat caaagaaaaa gcagattata ttacaaaaac attagaagaa gatggcattt
     5701 ttgatgcctt agaagtattt ggtatggtag aaaaagaatt gcattttcca caagtagaca
     5761 ttgaaacagt agaaggtcct cttgcgacta ttaagaccaa tcacggagac ttacgtatca
     5821 agcttttccc tgaacatgct cctaaaacag tggctaactt tgtatctctt tcaaaagatg
     5881 gctactatga tggtgtcatt ttccaccgta ttatcaagga ctttatgatc caaggtggag
     5941 acccaactgg aactggtatg ggtggcgagt caatctacgg cgaatctttt gaggatgaat
     6001 tctcagaaga gctttacaat atccgtggtg ctctttccat ggcaaatgct ggtccaaata
     6061 ccaacggcag ccagttcttt atcgtgcaaa accaacacct accttattct aagaaagaaa
     6121 ttactcgtgg tggttggcca gaaccgattg cagaaatcta tgccaatcaa ggtgggacac
     6181 ctcacctaga ccgccgtcac acggtttttg gtcagttagc tgatgaagca tcttacgctg
     6241 tcttggatgt cattgctgct gttgagacag gagctatgga caagccagtt gaagatgttg
     6301 taattgaaac tattgaaatc gaggactaag atgaaaatcg gtgataagct aaaggggcgt
     6361 attacaggga ttcagcccta cggtgccttt gttgagttag agacgggtga tacggggctg
     6421 attcacattt cagaaattcg gacaggattt attgaaaata ttcatgaaac cttgaaagtc
     6481 gatgaagagg ttcaagttca ggtagtggat ttagatgaat ttacggggaa agccagtctt
     6541 tctatccgca ctttggagga agaaaagtac cagtttccta gacggagacg cttttcaagc
     6601 gaccgcttta actacggttt tgctcccttt cgacgaatgc taccaatctg gactggagaa
     6661 gccctgcacc atttaaaaaa gaagaagtag tttagagttt tatatttttt gtaatgttaa
     6721 tataaatttg ctataatagg accatggaag aagaacaatt attaaaatca ggagagcgca
     6781 ttaaccagct cttttcgaca gatatcaaaa tcattcaaaa tagagaggtt tttagctatt
     6841 cggtggatag tgttctctta tcacgatttc cacgttttcc taagaagggg ttgattgtgg
     6901 atttctgtgc tgggaatgga gcagtggggc tttttgctag cactcgtact caagcacaga
     6961 tattgtctgt tgagattcag gagcgtttgg cggatatggc tgaacgctct gtccgtttga
     7021 atggattaga ggagcaaatg caggtcatct gcgatgattt gaaaaatatg cctgctcaca
     7081 ttcaggggag taaggtggat atgattttgt gtaatccacc ctatttcaag gtggatcctt
     7141 attctaatct gaacgagagt gaacattatc tcttggctcg acacgaaatc acgactaatt
     7201 tggaagaaat ctgtcatagt gcccagagta ttctcaagtc taatgggcgt ttggccatgg
     7261 ttcatcgtcc tgatcgactt ctggatattt tggatacgtt aaaacgacat aatctagccc
     7321 ctaagcgcct gcagtttgtt tatccaaaaa gagaaaagga agccaatatg cttttgattg
     7381 aggcgatcaa ggatggctca acaagtggct ttaaggtctt acctcctttg attgtccaca
     7441 atgatgatgg ctcttatacg cccgaaatcg aagagattta ttatggatca taaggcttat
     7501 atgtacgttc tggagtgtcg tgatggatcc tactatatag gctatacgac tgatatgaga
     7561 agacgcctcg ctatccacaa tagtgggaag ggagccaaat atacacgagc acgcttgcca
     7621 gtcaaactta tctatgctca aggttttgcc agtaaggaag aagccatgtc ggctgaagct
     7681 cttttcaagc gtaagaagag gccacagaag gaagaatttt tatctgaaaa tcaagataga
     7741 aatttactca gttattttga agaatcgtgg ggagtccttt gactcctctt acctttgtca
     7801 aaaagcgaaa aaaatgctac aataaagaaa ataaacaaaa tgaggtatta tcatgtccaa
     7861 gattctagta tttggtcacc aaaatccaga ctcagatgcc atcgggtcat ctgtagcttt
     7921 tgcctacctt gcaaaagaag cttacggttt ggatacggaa gctgttgccc ttggaactcc
     7981 aaatgaagaa acagcctttg tcttgaacta ttttggtgtg gaagcaccac gtgttatcac
     8041 ttctgccaaa gcagaggggg cagagcaagt tatcttgact gaccacaatg aattccaaca
     8101 atctgtatca gatatcgctg aagtagaagt ttacggtgtt gtagaccacc accgtgtggc
     8161 taactttgaa actgcaagcc cactttacat gcgtttggag ccagttggat cagcgtcttc
     8221 aatcgtttac cgtatgttca aagaacatgg tgtagctgtg cctaaagaga ttgcaggttt
     8281 gatgctttca ggtttgattt cagataccct tcttttgaaa tcaccaacaa cacacccaac
     8341 agataaaatc attgctcctg aattggctga attggctggt gtaaacttgg aagaatatgg
     8401 tttggcaatg ttgaaagctg gtaccaactt ggctagcaaa tctgctgaag aattgattga
     8461 catcgatgct aagacttttg aactcaacgg aaataatgtc cgtgttgccc aagtgaacac
     8521 agttgacatc gctgaagttt tggaacgcca agcagaaatt gaagctgcaa tgcaagctgc
     8581 caacgaatca aacggctact ctgactttgt cttgatgatt acagatatcg tcaactcaaa
     8641 ctcagaaata ttggctcttg gtgccaatat ggacaaggtc gaagcggctt tcaatttcaa
     8701 acttgaaaac aatcatgcct tccttgctgg tgccgtttca cgtaagaaac aagtggtacc
     8761 tcaattaact gaaagcttta atgcgtaaga ttttgggtgt cagctcaaaa tcggaaagtc
     8821 tagtttgcct tatatcgaaa ggagtttcgg ctcctttttt tctaggagtg aagtatgtta
     8881 gaaaatggcg atttgatttt tgtgagagat gggtcagaca tgggacaggc catccagact
     8941 tccacaggta actatagcca tgttgccatt tatttggatg ggatgattta tcatgctagt
     9001 ggacaggctg gtgctgtctg tcaagaaccg gcagacttct ttgagtccaa tcatttgtac
     9061 gacctctatg tttacccaga aatggatatc cagtcggtga aggaaagagc ttgcagacat
     9121 cttggagcac cctacaatgc ttctttctat ccagatgcag ctggtttcta ctgctcccag
     9181 tatatagcag aaatcctacc tatttttgaa actattccta tgaaatttgg agatggggaa
     9241 caggagatta gtgatttttg gagggagtat tacatagaac taggtctgcc tgttcctctg
     9301 aaccaagctg ggaccaatcc tagtcagttg gcagcatcgc ctctgttaca atgtaaagaa
     9361 aggaatcttc atgattcaga tttttaattc atctcgtttg acgagacagc catttttgga
     9421 gaattgatcc gctatctgga ccagtatgag gatgtgattc tacgggaaat taaggctcaa
     9481 tttccagatg ttgcagttga taaactcatg gaagagtata taaaggcaag cttgattcta
     9541 cgtgaaaata agcgctatta cctcaatttt cctacgcttg aatcacttga tagtcttgaa
     9601 ctggatcaag agatttttgt cagagaagct agtccggtct atcaagcctt gttggagcag
     9661 agttttgaga cggaattgcg caatcaaatc aatgcagcta ttttagttga aagacggact
     9721 ttgcgcgcat taaaatgacc ctgtccaatt atttttacaa ggtcaaacag cagtatcctt
     9781 tgacagaaaa acagcaggag ctctatgaca ttttaggaga tgttaatcct gagtatgcac
     9841 tcaagtatat gacggctttt ttgttgaaat ttctcaaaaa agaccagctt atgcagaaat
     9901 gccgtgatat ctttgtggac agtttggttg tcttaggcta tattgtgcaa aatgaagata
     9961 gaaagtatga gttggctatc gattttgata aggagagatt aactttctac ttagcgtgat
    10021 ttcttgtttc tgagcacatt gtttgacttt ccttagtatt cggtataaac tatatgtaac
    10081 cggtaacaca tatcggaata aactaaagga gacaatcata tgtcacttga aaacaaattg
    10141 gaacaagcaa caggcgctgt caaagaaggt tttggtaaag ttactggaga cagcaagaca
    10201 gaacttgaag gagctgttga aaaaacagtt gctaaggcaa aagacgttgt agaagacgca
    10261 aaaggtgctg tagaaggtgc cgttgaaggt ttgaaaaacg tttttactaa ataataggaa
    10321 aaaatcaagg gtttcatttt cccttgattt tttctattct tataaataat tttctgcgac
    10381 ggctgtatct cctgggtagg attctttctt gccctggatg atttggtaac aatcggctcc
    10441 cttacccgca ataataactg catctaattc gtgatttgtg atagccattg ccgccttgat
    10501 ggcttcttgg cgatccgcaa tcttttcaac aggatgattg atgtagctac taatttcatc
    10561 tgcaatggcc attgggtctt catagttagg gtcatcagca gtcagaaaga cttgaatctc
    10621 agggtgttga ttgaggagga ggccaaagtc cttacgacga ctttctccct tgtttcctgt
    10681 tgatcccaga accagagcaa tctttccggt ttgatgagtt tcaaccacat tgatgagttt
    10741 tttcagacta tccccattgt gggcatagtc gatgaagacc ttggctccat ttttctgagt
    10801 gaggacttcc atacgaccag gaacgcgggt tgcagcgatg ccttttttga tgtcctcaag
    10861 acttgctccg agacggagac aagcaagtcc agcagcaact gcattttctt ggttgaagtt
    10921 gccaatgagt tggatatcat aatctccagc gagtttaccc gtagctgaaa agctaaaggc
    10981 tttggaattc tcgatttggt tatcaaattg gctaccatag aaatcatggt cttgatcttc
    11041 aacctgttct ttcaagactg agaagtggtc catgtcactg ttaatgatga ctgctcggct
    11101 attttccatc aagagacgct tgtggtagaa atagtcttca aagctagggt gttcaatcgg
    11161 gccgatatgg tcaggagtga tgttaagaaa gactcctaca tcaaaggtca gtccatagac
    11221 tcgatggact agataggctt gactggagac ttccatgatg aggtgggtac ggtcattttg
    11281 cacagcctga ttcatcatgt caaagaggtc aatactctca ggggttgtca acgctgactt
    11341 aaagaaagtc tcgccatcaa gagttgtgtt catggtcgac aacatagcag gtctatgccc
    11401 ttgagataag atgttatagg cgaaataggt tgctgttgtc ttacccttag taccagtaaa
    11461 ggcaaggagt ttgagtttct cttgtggatt accatagaac tccatggcaa tcaaactcat
    11521 ggctttcttt atatcgttca caatgatgac agggatatcg acttcgtagt ccttttcagc
    11581 tacataccaa gctaaacctt gtgttatagc agaaagaagg tattcttttt taaaggcagc
    11641 gccttttgca aaaaaaagag tgtcttctgt tacttttcgg ctgtcgtagc tgatgctatc
    11701 aaaaataact ttgctgtagt tgtagtggta atgaccttgg tcaataattt cgcgaaaaag
    11761 gccatctttc tttaaaatat ctaatacggt ttcaatctta atcatacttt ctattgtaaa
    11821 ccgaaagtcg taaatttaca agtaacaagg aaaagtttat aatggaagat aaggagtttt
    11881 tcctagttat caaaattgaa tgaggaatct atgtcgcacg aaaacaatca ccagcaggcc
    11941 cagatgttac gggggactgc ttggctaacg gctagtaact ttatcagtcg cctactcggg
    12001 gctgtttaca ttatcccttg gtacatctgg atgggggctt atgcagctaa ggcaaatggt
    12061 ctctttacca tgggttacaa tatctatgct tggttcttgt tggtttcaac agcggggatt
    12121 ccagttgcgg tggccaagca agttgccaag tataatacca tgcgagaaga agagcatagc
    12181 tttgccctga ttcggagctt cttaggcttt atgacaggac taggcctggt ttttgcttta
    12241 gtcttgtatg tctttgctcc ttggctagca gacttgtctg gcgtgggcaa agacttgatc
    12301 ccaatcatgc aaagcttggc ttggggagtc ttgattttcc cgtctatgag tgttatccga
    12361 ggatttttcc aagggatgaa taacctcaaa ccctatgcca tgagccaaat tgctgagcag
    12421 gtcattcgtg ttatctggat gctcctagca acctttatca ttatgaagct cggttcagga
    12481 gattatctag cagccgttac ccaatcaacc tttgctgcct ttgtcggtat ggtagccagt
    12541 tttgcagtct tgatttattt ccttgcccaa gaaggttcac tcaaaagagt ctttgaaaca
    12601 ggagataaga ttaacagtaa gcgtctcttg gttgatacca ttaaggaagc cattcctttt
    12661 atcctgacag ggtctgccat ccagctcttc cagattttgg atcagctgac ctttatcaat
    12721 agtatgagct ggtttaccaa ctacagcaat gaggacttgg ttgtcatgtt ttcttatttc
    12781 tcagccaatc ctaataaaat cacgatgatt ttgatttctg taggggtttc gattgggagt
    12841 gttggtttgc cacttttgac ggaaaactat gtcaaggggg acttgaaagc agcttctcgt
    12901 ctcgttcagg acagtctcac cctactcttt atgttcttgc taccagcaac ggttggagtg
    12961 gttatggtag gagaacctct ttatacggtc ttctatggta agccagatag tttggctctg
    13021 ggcttatttg tctttgcagt tttgcagtct attattttag gcttgtacat ggtcttgtct
    13081 ccaatgcttc aggccatgtt ccgcaaccgc aaggccgttc tctattttat ctatggttct
    13141 attgccaagc tagtcttgca actacctacc atcgccctct tccacagtta tggtcctttg
    13201 atttcaacaa ccattgctct catcattcct aacgtcttga tgtatcggga tatttgtaaa
    13261 gtaactggtg tcaagcgcaa ggtgattttg aagcgaacca ttttaatcag tttgctgacc
    13321 ctagtcatgt ttctgttaat aggaaccatc cagtggctgt taggattttt cttccaacca
    13381 agtggacgtt tgtggagctt cttttatgta gctcttgtcg gtgccatggg gggtggactt
    13441 tatatggtta tgagtctgcg tacctattta ttagataagg taataggaaa agcccaagca
    13501 gatcgcctgc gagcaaaatt taagctttcg taaggtagca tttataaata aaaggaataa
    13561 gttgaacaat cttttaggag aacttgaagc ttattccttt tttagcgtaa aaatagctaa
    13621 aattatattt caaaaataga aaatgaggct tttttcgatt ttttggaaaa aatcattgac
    13681 taaacaaact aaacgttgta taataaaaca aatatttaaa gcaggaggtt ccggaaatga
    13741 aaaagtctaa gagcaaatat ctaaccttgg caggtcttgt cctgggtaca ggagttttat
    13801 tgagcgcgtg tggaaattct agcacggcgt caaaaacata caactatgtt tattcaagtg
    13861 atccatctag cttgaactat ctagcagaaa accgcgcagc aacatccgat attgttgcaa
    13921 atttggtaga cgggttatta gaaaatgacc aatatgggaa tattattcca tcattagcag
    13981 aggattggac tgtttctcag gacggtttga cctataccta caaacttcgt aaggatgcca
    14041 agtggtttac ttctgaggga gaagaatatg cgcctgtaac tgcccaggat tttgtgacag
    14101 gtttgcaata tgcagctgat aaaaaatcag aagccttgta tctagtgcag gactctgttg
    14161 ctggtttgga tgactatatc actggtaaaa caagcgactt ttcaactgtc ggtgtcaagg
    14221 cacttgatga ccaaacggtt caatatactt tggttaaacc agaactttac tggaattcaa
    14281 aaacacttgc aacgatactt tttcctgtta atgcagattt cctgaaatca aaaggggatg
    14341 attttgggaa ggcggatcca tctagtattt tgtacaatgg acctttcttg atgaaagcac
    14401 ttgtctcaaa atctgctatt gaatataaga aaaaccctaa ttactgggat gctaagaatg
    14461 tctttgtaga cgatgtgaaa ttgacctact atgatggtag cgaccaagaa tcactggaac
    14521 gtaattttac agctggtgct tatactacgg ctcgtctttt tcctaacagc tccagctatg
    14581 aagggattaa agaaaaatac aaaaacaata tcatctatag tatgcaaaat tcaacttcat
    14641 atttctttaa ttttaaccta gataggaagt cttacaatta tacttctaaa acaagtgaca
    14701 ttgaaaagaa atcgactcag gaagcagttc tcaataaaaa cttccgtcag gctatcaatt
    14761 ttgcttttga cagaacatct tatggggctc agtctgaagg gaaagaaggt gcaacaaaga
    14821 ttttgcgtaa cctagtggtt cctccaaact ttgtcagtat caagggaaaa gactttggtg
    14881 aagttgtagc ctctaagatg gtcaactatg gtaaggaatg gcaaggtatc aactttgcgg
    14941 atggtcaaga cccttactac aatcctgaga aagccaaggc taagtttgcg gaagctaaga
    15001 aagaactcga agcaaagggt gttcaattcc caatccactt ggataagact gtggaagtaa
    15061 cagataaagt aggcatacaa ggagttagtt ctatcaaaca atcaattgaa tctgttttag
    15121 gttctgataa tgtagtgatt gacattcagc aattaacatc agatgagttt gacagttcag
    15181 gctactttgc tcaaacagct gctcagaaag actatgattt atatcatggc ggttggggac
    15241 ctgattatca agacccgtca acctatctcg atatttttaa tactaatagt ggaggatttc
    15301 tgcaaaatct tggactagag cctggtgagg ccaatgacaa ggctaaggca gttggactgg
    15361 atgtctatac tcaaatgttg gaagaagcta ataaagagca agatccagcc aaacgttatg
    15421 agaaatatgc tgatattcaa gcttggttga ttgatagttc tttagttctt ccaagtgttt
    15481 cgcgtggggg aacaccatca ttgagaagaa ccgtaccatt tgctactgcc tatggtttaa
    15541 ccggtacaaa aggggttgaa tcatataaat acctcaaagt acaagataag attgtcacaa
    15601 cagacgaata tgcaaaagcc agagaaaaat ggttgaaaga aaaagaagaa tccaataaaa
    15661 aagcccaaga agaattggca aaacatgtca aataaaatct aattgtagat aagtttgtgt
    15721 aagaagtctt tgtgcttaaa aacgcataat atcaggtata taaaatccta gatgttatgc
    15781 gttttgttat aggaagattt attagatttt atcctcaaat tgagttattc ccgactttat
    15841 ccgttgaaat attataagga gtatttatga aattaaaatt attaagagta gatactaagg
    15901 tgattatggg gagtttctta cttgttctgt ctagtctact tgctttgttg cttcccctta
    15961 tcttaaaggg tttaatagat gggagttcta ttgaaaatat aggttccaaa gtatttcaat
    16021 cgtttttgat ttttattggt caagccttgt tttcttctat tggttactat ctgtttagtc
    16081 aatcgggtga aaaaaagata gcaaaaatca ggaaaaaagt gatagagggg ttgatttatg
    16141 cagagaaatc cttctttgat aagagccaaa gtggggagtt gacttctgcc attgtcaatg
    16201 acacgagtgt cattcgtgag tttttaatta cgactttccc aaatattatt ctgagtttag
    16261 ttatggtact tggttccatt gtagtcttat ttagtcttga ttggaatctt tctctacttt
    16321 tattcatcac tcttccttgt atgatgttta ttatcttgct cctttccaat atcagtgaaa
    16381 agtatagtcg tcgtttacag gaggaaatcg gatttttaac agggcaattg actgaaaaga
    16441 ttcaagaaca tgaattaatt aagactaacc aagcagagaa aagtgtacag gatgttttgg
    16501 ataattgtat tgaaagggta caaaacaatt cactgaaatc agatagggtt acttcttttg
    16561 agaccccgtt tgctctctta tttatctttg cgactatagc tgtgatgcta acctatgggg
    16621 gttatcgggt cagcgcagga tatatatctg tgggaacctt ggtttcgttt ttgatttacc
    16681 tctttcaatt acttaatcct attagtaata tagctaattt tgtaactgtt tattctagga
    16741 gcaagggatc ttcagttgca ctggagaact tgcttgcagt tcctaaagaa aaatttgagt
    16801 gaggaaaatc ggtatcagga caagggttga attttaacca tgtctatttt ggttatgatg
    16861 aaaatcgacc tgtcttaaag gatattactt gttcaatttt caaggggcaa aaaattgctt
    16921 ttgttggacc atctggatca ggaaaatcaa cgattgtgcg tttgttagag cggttttata
    16981 aaccgctttc aggagatatt ctaatggagc aatcaagtat atatgatttt aacttaaaag
    17041 aatggagaag taaaatcgct tagtaactga tgatgaattg atgaaagtgc tagacttagt
    17101 atcactaggt gatgagattc gctccatgaa agagggacta gatactgaag ttggtgaacg
    17161 cggacgactc ttgtcagggg ggcaaagcca aagacttcaa atagctagag cctacttaaa
    17221 agatgctgaa attcttatat ttgatgaagc cactgctaat cttgatgcgg attctgagta
    17281 tgcgattatc agtagcctct attctgtatt aaaggagaag acggttgtga ttatagcgca
    17341 tcgtttgtca acggtaaaag atgtggattg tattttcttc ttagaggagg ggaaaatcac
    17401 tggctcagga actcataagg aactactgga aaatcatgag cgttatgctc gttttgtgca
    17461 ggagcaaatg atagagtgaa ggtgtctttt gagattcacc attttatagt ctattaaagg
    17521 gagcaggaaa aactcccttt ttatatagtt tgaaactata actagctctt gaaaagaaga
    17581 aaatgagttg atgaaaataa gtggtacaat agttactata gatttggagg tattgtatga
    17641 gcaaggaatt acacattaac acaattttgg cccaggcggg tattaagtca gatgaagcga
    17701 caggtgcatt ggtgacaccg cttcattttt caacgaccta tcagcatcca gagtttggtc
    17761 gatctactgg gtttgactat acgcgcacta aaaatccaac tcgtagtaag gctgaggaag
    17821 tcttggcggc tattgagtca gcagactatg ccctagcgac tagctcaggg atgtcagcta
    17881 ttgtactggc ctttagcgtc tttccagtag gaagtaaggt cttggcagtg cgtgatcttt
    17941 acggtggttc ttttcgctgg tttaaccaag tggagcagga aggtcgtttc cattttacct
    18001 atgccaatac agaagaagag ttgattgccg agttagaaaa ggatgtggat gttctctata
    18061 tcgaaacccc aaccaatccc ttgatgttgg aatttgatat cgaaaaacta gcaaaattgg
    18121 ctcatgctaa gggtgccaaa gtggtggtgg acaatacctt ctatagccct atctaccaac
    18181 gtccgattga agatggagca gatatcgttc tccattcagc aaccaagtat ctagcaggcc
    18241 acaatgatgt cttggctgga gtggttgtga ccaatagttt agaactatac gagaagcttt
    18301 tttacaatct caatacaaca ggggcagtct tgtctccatt tgacagctac cagttgcttc
    18361 gtggtctcaa gaccttatct cttcgtatgg agcgctcaac agctaacgcc caagaagtgg
    18421 ttgccttttt gaaggattct ccagcagtta aggaagttct ctacactggt cgtggaggca
    18481 tgatttcctt taaagtagcc gatgaaacac gcattcctca tattttgaac agtctcaagg
    18541 tcttctcttt tgcggaaagt ttgggcggag tggaaagtct tattacttat ccaacgactc
    18601 aaactcatgc tgatattcca gcagaagtac gccattctta tggtttgaca gatgacctct
    18661 tgcgtttgtc tattgggatt gaggatgcta gagatttgat tgcagatttg cgccaagcct
    18721 tagaaggata agacaaagat gggaaaatat gattttacaa gcctgcccaa ccgtttaggg
    18781 caccatacct ataaatggaa agaaacagaa acggatagtg aagttctacc agcttggata
    18841 gcggatatgg actttgtggt cttgcctgaa atccgccaag ccgtgcaaac ttacgcagac
    18901 caactggttt atggttatac ctatgccagt gaagacttaa ttaaggaaat tcaaaagtgg
    18961 gaagctacac aatacggtta caactttgac aaagaggctc ttgtctttat cgagggtgtg
    19021 gtaccagcca tctcaacagc tattcaaacc tttacaaaag aaggcgaggc ggttttaatt
    19081 aacacgcctg tctacccacc ctttgctcgc agtgtcaagt tgaataatcg tagattgatt
    19141 actaattcct tagtggaaaa ggatggtctg tttgagattg actttgacca acttgaaaag
    19201 gatttggtgg aagaggaggt taaactctat attctttgca accctcacaa tcctggtgga
    19261 cgtgtttggg aaaaagaagt gttggagaag attggccaac tttgccaaaa acacggtgtt
    19321 ttgttagttt cggatgagat tcaccaagat ttgaccctct ttggtcacaa acaccagtct
    19381 ttcaatacca tcaatcctgc cttcaaaaat tttgctatcg tcttgagcag tgccactaaa
    19441 acatttaata ttgctggaac aaaaaattcc tatgcagtca ttgaaaatcc taagttgaga
    19501 ctggctttcc agaaacgcct gttggccaat aatcagcatg aaatttcagg cttgggttat
    19561 ttggcgacag aagctgccta tcgatacggt aaagattggc tagaggaact caagcaagtc
    19621 tttgaagacc acatcaatta tgtggtggat ctatttggaa aagagactaa aatcaaggtc
    19681 atgaaaccgc aaggtaccta cttgatttgg cttgactttt cagcctatga cctgactgat
    19741 gaaacattgc aagagttgtt gagaaatgaa gccaaggtta tcctcaaccg tggtttggat
    19801 tttggagagg aaggaagtct ccatgcccgc atcaatatag ctatgcccaa atctctgttg
    19861 caagaagtct gtcagcggat tgtggctact tttgccaaac gttaaaaatc cagccttcta
    19921 ggagaaaagt cttcctagaa ggctattttc ataggcgaaa atatggtata ataaaaagat
    19981 aaggtaaagg tgaaaatatg gctaaattga ttccggggaa aattcgtatc gaaggtgttg
    20041 ccctttatga aactggtaag gttgatatca tcaaggaaaa gaacaatcgg ctctacgctc
    20101 gcgttgcaga agaagaactg cgctatagtt tagaggatga tttggttttt tgtgcctgtg
    20161 attcttttca aaagaggggc tactgtgtgc atttggcagc gctagagcat tttctgaaaa
    20221 atgatgagcg tggtcaggaa atcttgtgga gtctggaaga aggtcatgaa gaaaaagagg
    20281 ccgttgaaac caaggtgacc ttgggtggca agtttttgaa tcgaatttta tctccaaaat
    20341 cagaatgcgc ctatgagtta tcagctgtgg gtcaggtgga agcaggaacc aatcatatct
    20401 tgtggactct gagaatcggc caaatcaata gccaaaaata ctatgtcatt cgggatattc
    20461 cacttttttt aaggattgtc gagcagagaa agtcgtatat gattggcaag atttatgagg
    20521 agtctctttc ttgggaagcc tttgatgatg ccagccaaga acttttgact ttcttgcacg
    20581 gactaacgga ggaagggctg gttccagacc tctttttcca aaaccaaggt cggcacctct
    20641 ttttccctct gacctttttt gagcagggtg tgaatttact gatggccttg ccccattttc
    20701 aatttgacca tcaagtggat agctaccaaa ccctgctttt tcaggatatg catgctgatg
    20761 ccaatctatt tgcctttaca gtaacagaat actcggacta ttttgaaatg gaaatcagtg
    20821 agagtcctag ggtcaatgtc ttttatcagg gagcagtact tttccataaa ggtcaggttt
    20881 attttttaac agaccagcag atgcgtcttc tcaaggaaat taaagcgtta cctgtgggtc
    20941 aacaaggaaa gaaatacctg caatttgata gcagtgatcg agacaaattg gcctcctgtc
    21001 tgactctttt tatccagatg ggcaccgttt cagctccaga acgtctacaa atcaaaactt
    21061 ttgcgccctc tttttacttt gacagggaag aagataatcg gattcgttta gaaattcagt
    21121 ttgattatgg aaatagacag gtatctagcc gacaagagct agaagaatta ccattttcaa
    21181 gtgacgcgga cttagaagaa agaattttcc aagtctgttt ggctgcgggc tttgaagccg
    21241 attttcaatc ttggcgtcag gccttaaaag cggagtctgt ctatcatttt tttcatgata
    21301 tcattccagt ctttgaaaaa ctcgggcatg ttgacctatc agataaacta gaagagcttt
    21361 atagcctagc gagtcctcag gtgcagattg cctccaaggg aggtctcttg gaaatccagt
    21421 ttgattttca aaatattgcc caggaggaaa tcgaccaagc catgcaggcc ttggttgcca
    21481 atcaggattt ttatattgat tcgtctaatc aagtttactt tttcgatgaa gaaaccaaga
    21541 aaattcgcca aaatctacag gaactggggc aatttgaatt aaaagatggg accttgcagg
    21601 ctcggaaatc cttggcctac agtttagctc atctctttga agggcgagac cgtgtttctt
    21661 tttcacaaga attccagaat ctggcccagg atttgacgca tccagaggac tttcctttgc
    21721 aagcaactca ggtcaaggct gacttgcgag attatcagga aaagggaatt ggctggttgc
    21781 agatgctcca tcattatggt tttggtggga ttttggctga tgatatggga cttgggaaaa
    21841 cccttcagac cattgctttt ttgactagtc aagtgacaaa agaaagtcga gtcttgattc
    21901 tcgctccgtc gggtttgatt tacaactggg cggatgagtt tcagaaattt gccccacagt
    21961 tggatgtggc tgttgttcat ggtttgaaag caagtcgtga ggagattctt gccgagagcc
    22021 atcaaatcta tgtgactagc tatgccacct tccgtcagga cagtgagttt tatcagggga
    22081 tggcctttga cttccttttc ttagatgagg ctcaggtcat gaaaaatgcc cagaccaaga
    22141 ttgcccagac cttgagacaa tttgtggtgc cgtcggtctt tgccttgtca ggaactccga
    22201 ttgaaaacca tctgggtgag ttgtggtcta ttttccaaat cgtcatgccc ggacttttgc
    22261 caagcaagaa agaatttatg aaattgccag cagagcgagt ggctcagttt atcaagcctt
    22321 tcgtgatgcg acgcaagaaa gaagaagttc tgactgaatt gccagacttg attgaggtgg
    22381 tttataaaaa tgaactggaa gaccagcaaa aggctattta ccttgcccag ttacaacaga
    22441 tgcgagacca tctggctcaa gtgtcagaac aggaatttca gcgaagtcgt gtggaaattt
    22501 tatctggttt gatgcgcttg cgtcaaatat gtgacactcc tgccctgttt atggaagatt
    22561 atcagggagc cagcggcaaa ctagatagtc tccgagacct actggtacag gtggcagacg
    22621 gcggacaccg tgtcttgatt ttctctcagt tcaagggaat gttggaaaaa attgaacaag
    22681 aactgccaga cttgggcctg acttccttta aaattacggg ttcaacccca gccaaggaaa
    22741 gacaagacat gaccaaggcc tttaatcaag gagaaagaga tgcctttctg atttccctca
    22801 aggctggtgg ggtcggtctg aacctgacag gtgcagatac agtgatcttg gttgaccttt
    22861 ggtggaatcc tgcggtggaa gcgcaagcca ttggccgtgc ccatcggatg ggtcaggaag
    22921 aaacggttga ggtctatcgc ttggtgacca agggaaccat tgaagaaaaa attcaggaac
    22981 tccaagaaca aaagaaacat ctggtgtcac aagtattgga cggcacagag tcacgtggta
    23041 gtctgaccct agcagaaatt agagaaattt tgggaatttc tgaagccagc acttgaaaaa
    23101 tcaagacaat acgctataat gaagtgttga aaggaaatag aaaatgaaac aagaacgatt
    23161 tccattggtg tcagatgacg aggtcatgtt gactgaaatg ccagtcatga atctctatga
    23221 tgagtctgat ctgatcagta atatcaaggg tgagtatcga gataaaaatt atttagaatg
    23281 ggctcctatt gctgaagaaa aaccagtaaa accgattgaa aagcaagtag ggaaacctaa
    23341 aaaagctcct ttaggggtta aaaaagaagg aaagagctat gcggaggtgg cgcgtgaaga
    23401 agcgcgtgca gacttgaaaa agaaacgctc tgctaactat ctaactcagg atttcagcct
    23461 tgcgagacgt cattctcagc ccagtctagt tagacagggc aatcaaccga caactccttt
    23521 ccaaaaggaa aatcctggtg aatttgtcaa atatagccaa aaattgaccc agtctcatta
    23581 tatcttggcg gaagaagttc attctatccc taccaagaat gaagaagtgt cagcacctgc
    23641 tccaaagaaa aacaattatg attttctaaa gaagagccaa atctacaata aaaaaagtaa
    23701 acaaacagaa caagaacgtc gggttgccca agagttgaat ctgaccagaa tgacagaata
    23761 gggagaaaac atgtcaaaga catatcattt tatcggaatt aagggatcag ggatgagtgc
    23821 cttggccttg atgttgcacc agatggggca caaggttcag ggatcagatg ttgaaaagta
    23881 ctactttacc caacgcggtc ttgagcaggc aggaattacc attcttcctt ttgatgaaaa
    23941 gaatctagac ggtgatatgg aaattatcgc tggaaatgcc tttcgtccag ataacaacgt
    24001 cgaaattgcc tatgcggacc aaaatggtat cagctacaaa cgttaccatg agtttctagg
    24061 tagctttatg cgtgactttg ttagcatggg agtagcagga gcacatggaa aaacttcaac
    24121 gacaggtatg ttgtctcatg tcttgtctca cattacagat accagcttct tgattggaga
    24181 tgggacaggt cgtggttcgg ccaatgccaa atattttgtc tttgaatctg acgaatatga
    24241 gcgtcacttc atgccttacc acccagaata ctctattatc accaacattg actttgacca
    24301 tccagattat ttcacaagtc tcgaggatgt tttcaatgcc tttaacgact atgccaaaca
    24361 aatcaccaag ggtctttttg tctatggtga agatgctgaa ttgcgtaaga ttacgtctga
    24421 tgcaccaatt tattattatg gttttgaagc tgaaggcaat gactttgtag ctagtgatct
    24481 tcttcgttca acaactggtt caaccttcac cgttcatttc cgtggacaaa acttggggca
    24541 attccacatt ccaacctttg gtcgtcacaa tatcatgaat gcgacagccg ttattggtct
    24601 tctttacaca gcaggatttg atttgaactt ggtgcgtgag cacttgaaaa cattttccgg
    24661 tgttaaacgt cgtttcactg agaaaattgt caatgataca gtgattatcg atgactttgc
    24721 ccatcatcca acagaaatta ttgcgacctt ggatgcggct cgtcagaaat acccaagcaa
    24781 ggaaattgta gcagtctttc aaccgcatac ctttacaaga accattgcct tgttggacga
    24841 ctttgcccat gctttaaacc aagcagatgc tgtttatcta gcgcaaattt atggctcggc
    24901 tcgtgaagta gatcatggtg acgttaaggt agaagaccta gccaacaaaa tcaacaaaaa
    24961 acaccaagtg attactgttg aaaatgtttc tccgctccta gaccatgaca atgctgttta
    25021 cgtctttatg ggagcaggag acatccaaac ctatgaatac tcatttgagc gtctcttgtc
    25081 taacttgaca agcaatgttc aataggatgt tcccatggaa attccaatta agatcattca
    25141 ggcaagcaag tctgatttgc ctgagatagg ggcacttcag acctcgtctt ttccagctga
    25201 aaagcagcaa ctttcccata ttttagaaga aagtatccgt aagtgtgcgg atacctttct
    25261 tctagctagg gatgaaaatc aacttttagg ctatatttta tcaagtcccc agtcagacaa
    25321 tccgcaatat ctaaaagtac attctttagt catcgagtct gaccatcaga gacagggctt
    25381 gggaacactt cttcttgcag ccttgaaaga ggtggcagtt gatctggatt acaaagggat
    25441 tcgtttggag agtcctgatg agctgctttc ctattttgaa atgaacggtt ttgttgatga
    25501 agaagcaact ttgctctatg caactagcca gggctatagt atgatttggt ttaatccctt
    25561 ttatctggag gaacaatgaa aatcagacaa gcaagattag aagatttgga tcggattgtt
    25621 gaacttgaat ttgaaaattt ttcggtcgaa gaagccattc ctccttctgt ctttgaagca
    25681 catttgagag aaattcagac ctcttttctg gttgctgaaa aagaaggaag aatcatgggt
    25741 tatatcgaag gaccagttgg cctgcaccgc catctgcaag accagtcttt tacagaagaa
    25801 ataaaagact atagtcatga gcctggtggt tatatctctg tgacctgtct gtctattgcc
    25861 aaggaggcac agggactcgg actgggtcag aaattgctga cagctttgaa agaagttgct
    25921 cttgaagatg agagagatgg cattaatcta acctgtcatg actatctcat cgcctattat
    25981 gaaaaacatg gatttgtcaa cgaaggccag tcccagtcaa cctttgcagg ggaaacatgg
    26041 tatgatatgg tctgggaaat gaaaaaataa gttaggaaaa gtatcataga ttgctttttt
    26101 tggactttta agatataata ttatggatta tcaacaagga ggaaaaactt ttgagtgaaa
    26161 agtcaagaga agaagagaaa ttaagcttta aagagcagat tctgagagat ttagaaaaag
    26221 taaaaggcta tgatgaagtt ctgaaagaag atgaggcagt agttcgcact cctgcaaatg
    26281 aaccttcagc tgaagaactc atgactgatt ccttgtcaac ggtagaggag attatgagaa
    26341 aagctcctac cgtgcctact cacccaagtc aaggtgtacc agcttctcca gcagatgaga
    26401 ttcaaagaga aactcctggt gttccaagtc atccaagtca agatgtacct tcttctccag
    26461 cggaagaaag tggatcaaga ccaggtcctg ttagacctaa gaaacttgaa agagaataca
    26521 atgaaacccc aacaagggta gctgtttcct atacgacggc agagaaaaaa gcagaacaag
    26581 caggtccaga aacacctacg cctgctacag aaacagtgga tatcatcagc gatacatcac
    26641 gtcgtagccg tagagaagga gcaaaacccg ttaatcctaa gaaagagaag aagtcacatg
    26701 tgaaagcttt tgtgatttca ttccttgtat tccttgcctt gctctcagca ggtggttact
    26761 ttggttacca gtacgtgcta gattctttgt tacctatcga tgctaattct aagaaatatg
    26821 tgacggttgg aattccagaa ggttcaaacg ttcaagaaat cggtacgacg cttgaaaaag
    26881 ctggtttggt aaagcatggt ctgattttta gtttttatgc caagtataaa aattataccg
    26941 acttgaaagc aggttactac aatttgcaaa agagtatgag tacagaagac ttactcaaag
    27001 agttgcaaaa aggtggaaca gatgaaccgc aagaacctgt acttgcgact ttgacaattc
    27061 cagaaggtta taccttggat cagattgctc aagctgtggg tcaattgcaa ggtgacttca
    27121 aagagtcttt gacagcggag gctttcttgg ctaaagttca agatgagacg tttatcagtc
    27181 aagcagtagc gaaatatcct actttactgg aaagtttgcc tgtaaaagac agcggtgcgc
    27241 gttatcgttt ggaaggatac cttttcccag ctacatactc tatcaaggaa agcacaacta
    27301 ttgagagctt gattgatgag atgttagctg ctatggataa gaacctatct ccttactata
    27361 gtactatcaa atctaaaaat ttgactgtca atgagttgtt gaccattgct tccttggtcg
    27421 aaaaagaagg tgccaagaca gaagatcgta agctcattgc aggtgtattc tacaatcgtt
    27481 taaatcgtga tatgccactt caaagtaata ttgcaatctt gtatgcccaa ggaaaactgg
    27541 ggcaaaatat cagtctagct gaggatgttg cgattgatac caacattgat tcaccttata
    27601 atgtttataa aaatgtaggt ctcatgcctg gtccagtcga tagtccaagt ctggatgcga
    27661 ttgagtcaag catcaatcaa actaagagcg ataacctcta ctttgtagca gatgtcacag
    27721 aaggcaaggt ctactatgct aacaatcaag aagaccacga ccgcaatgtc gctgaacatg
    27781 tcaacagcaa attaaactaa acaaactaaa attatgtgat acgtcacata attttcttta
    27841 gaaaatatta tcagaagaaa gttgagaaaa atggcagaaa aaacatatcc tatgaccctt
    27901 gaggaaaagg aaaaacttga aaaagaatta gaagaattga aattggttcg tcgaccagaa
    27961 gtggtagaac gcattaagat tgcccgttca tacggtgacc tttcagaaaa cagtgagtac
    28021 gaagcagcta aggatgaaca agcctttgtc gaaggacaaa tctctagctt agaaacaaaa
    28081 atccgctatg ctgaaatcgt caatagcgac gcagttgccc aggacgaagt agcgattggt
    28141 aaaacagtca ccatccaaga aattggtgag gacgaagaag aagtttatat tatcgtaggt
    28201 tcagctggtg cggatgcctt tgcaggtaag gtttcaaatg aaagcccaat tgggcaggcc
    28261 ttgattggca agaaaacagg tgacacagca accattgaaa cgcctgttgg tagctatgat
    28321 gtaaaaatct tgaaggttga aaaaacagcc taaaaacaga aaaaggagtg gggaggcgat
    28381 gtgcttcact cactcctttt tccattttgc ta
//
LOCUS       DAVBHS010000010        25235 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVBHS010000010 DAVBHS010000000
VERSION     DAVBHS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25235
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.010"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            218..448
                     /locus_tag="VQ651_000293"
     CDS             218..448
                     /locus_tag="VQ651_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HET2753340.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAIKC"
     gene            852..1565
                     /locus_tag="VQ651_000294"
     CDS             852..1565
                     /locus_tag="VQ651_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HET2753341.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            1623..2225
                     /locus_tag="VQ651_000295"
     CDS             1623..2225
                     /locus_tag="VQ651_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753342.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRFFSNFDDFLEKNYLKTIWQ
                     VSKETPFSEMDFNMFKNISEKIIFERGSKMLNDLKSNYKK"
     gene            2361..3014
                     /locus_tag="VQ651_000296"
     CDS             2361..3014
                     /locus_tag="VQ651_000296"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET2753343.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            3024..3488
                     /locus_tag="VQ651_000297"
     CDS             3024..3488
                     /locus_tag="VQ651_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2753344.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            3501..4769
                     /locus_tag="VQ651_000298"
     CDS             3501..4769
                     /locus_tag="VQ651_000298"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET2753345.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            4783..5043
                     /locus_tag="VQ651_000299"
     CDS             4783..5043
                     /locus_tag="VQ651_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753346.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            5054..6415
                     /gene="pdrM"
                     /locus_tag="VQ651_000300"
     CDS             5054..6415
                     /gene="pdrM"
                     /locus_tag="VQ651_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HET2753347.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            6569..7537
                     /locus_tag="VQ651_000301"
     CDS             6569..7537
                     /locus_tag="VQ651_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET2753348.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            7553..8545
                     /locus_tag="VQ651_000302"
     CDS             7553..8545
                     /locus_tag="VQ651_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET2753349.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDKNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYISEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            8763..9806
                     /locus_tag="VQ651_000303"
     CDS             8763..9806
                     /locus_tag="VQ651_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HET2753350.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            9870..11555
                     /gene="lpdA"
                     /locus_tag="VQ651_000304"
     CDS             9870..11555
                     /gene="lpdA"
                     /locus_tag="VQ651_000304"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET2753351.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEIIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMQSPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEIIGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            11619..12608
                     /locus_tag="VQ651_000305"
     CDS             11619..12608
                     /locus_tag="VQ651_000305"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HET2753352.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(12675..13745)
                     /gene="xerS"
                     /locus_tag="VQ651_000306"
     CDS             complement(12675..13745)
                     /gene="xerS"
                     /locus_tag="VQ651_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HET2753353.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
     gene            complement(14329..15879)
                     /locus_tag="VQ651_000307"
     CDS             complement(14329..15879)
                     /locus_tag="VQ651_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET2753354.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFIIIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGSLVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(15895..16674)
                     /locus_tag="VQ651_000308"
     CDS             complement(15895..16674)
                     /locus_tag="VQ651_000308"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET2753355.1"
                     /translation="MATIKEIKELLVTVKELESPIFLDLEKDNRSGVQKEISKHKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQVVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(16661..17512)
                     /gene="ylqF"
                     /locus_tag="VQ651_000309"
     CDS             complement(16661..17512)
                     /gene="ylqF"
                     /locus_tag="VQ651_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HET2753356.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EGLDGND"
     gene            complement(17719..17895)
                     /locus_tag="VQ651_000310"
     CDS             complement(17719..17895)
                     /locus_tag="VQ651_000310"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2753357.1"
                     /translation="MQAGIPTDNEVSYALIYQQETLPATGSSTSALTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            complement(17947..23838)
                     /gene="iga"
                     /locus_tag="VQ651_000311"
     CDS             complement(17947..23838)
                     /gene="iga"
                     /locus_tag="VQ651_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin A1 protease"
                     /protein_id="HET2753358.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGSIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSENTGEVSSNPSDS
                     TTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEKKLEL
                     RNVSDIELYSQTNGTYRQHVSLDGIPENTDTYFVKVKSSAFKDVYIPVASITEEKRNG
                     QSVYKITAKAEKLQQELENKYVDNFTFYLDKKAKEENTNFTSFSNLVKAINQNPSGTY
                     HLAASLNANEVELGPDERSYIKDTFTGRLIGEKDGKNYAIYNLKKPLFENLSGATVEK
                     LSLKNVAISGKNDIGSLANEATNGTKIKQVHVDGVLAGERGVGGLLAKADQSSIAESS
                     FKGRIVNTYETTDAYNIGGLVGHLTGKNASIAKSKATVTISSNTNRSDQTVGGLAGLV
                     DQDAHIQNSYAEGDINNVKHFGKVAGVAGYLWDRTSGEEKHAGELTNVLSDVNVTNGN
                     AITGYHYTGMKVANTFSSKANRVFNVTLEKDEVVSKESFEERGTMLDASQIVSKKAEI
                     NPLTLPTVEPLSTSGKKDSDFSKIAHYQANRALVYKNIEKLLPFYNKSTIVKYGNLVK
                     ENSLLYQKELLSAVMMKDDQVITDIVSNKQTANKLLLHYNDHSSEKFDLKYQTDFANL
                     AEYNLGNTGLLYTPNQFLYDRDSIVKEVLPELQKLDYQSDAIRKTLGISPEVKLTELY
                     LEDQFSKTKQNLGDSLKKLLSADAGLASDNSVTRGYLVDKIKNNKEALLLGLTYLERW
                     YNFNYGQVNVKDLVMYHPDFFGKGNTSPLDTLIELGKSGFNNLLAKNNVDTYGISLAS
                     QHGTTDLFSTLEHYRKVFLPNTSNNDWFKSETKAYIVEEKSTIEEVKTKQGLAGTKYS
                     IGVYDRITSATWKYRNMVLPLLTLPERSVFVISTMSSLGFGAYDRYRSSDHKAGKALN
                     DFVEENARETAKRQRDHYDYWYRILDEQSREKLYRTILLYDAYKFGDDTTSGKATAEA
                     KFDSSNPAMKNFFGPVGNKVVHNQHGAYATGDGVYYMSYRMLDKDGAITYTHEMTHDS
                     DQDIYLGGYGRRNGLGPEFFAKGLLQAPDQPSDATITINSILKHSKSDSTEGSRLQVL
                     DPTERFQNAADLQNYVHNMFDLIYMMEYLEGQSIVNKLSVYQKMAALRKIENKYVKDP
                     ADGNEVYATNVVKELTEAEARNLNSFESLIDHNILSAREYQSGDYERNGYYTIKLFAP
                     IYSALSSEKGTPGDLMGRRIAYELLAAKGFKDGMVPYISNQYEEDAKQQGQTINLYGK
                     ERGLVTDELVLKKVFDGKYKTWAEFKTAMYQERVDQFGNLKQVTFKDPTKPWPSYGTK
                     TINNVDELQALMDQAVLKDAEGPRWSNYDPEIDSAVHKLKRAIFKAYLDQTNDFRSSI
                     FENKK"
     gene            complement(24047..25225)
                     /locus_tag="VQ651_000312"
     CDS             complement(24047..25225)
                     /locus_tag="VQ651_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753359.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCRNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLPLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWILNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         7237 a         4843 c         5163 g         7992 t
ORIGIN      
        1 cttaacagat agtgtaaata aagataaact atttactggc taattaatca gttaaacact
       61 agttaaggag taatgatgaa aaaaagaaca atactattat tgatggccag tctgttagct
      121 cttgtcttag gagcatgtgg tttcttggac atattgatcc tggatcattc tcatcaggat
      181 tactctttac tgctatttta gaaactgggg tggtttgatg gaaagtattg gtattggtct
      241 tgttatcgtt tcacattcca aacacattgc agaaggtgtt gttgaactga ttagtaaagt
      301 agctaaagat gttccgatta cttatgtagg aggaaccgag ggcggaggaa ttggaacgag
      361 ttttgatcaa gtagataggg ttgtttccga aaatccagca gatactttac ttgccttttt
      421 tgacctaggt tctgctataa aatgttaagt aaatgatttc aaaatcagaa agggattgct
      481 ttgggcagtt cctttttaat gtaaatgtaa aaggagtaga atcatagtgt ttctaaattg
      541 tgaatcaatc aaaactgatt gtgatggggc tattctagct ttagaatcct tcaaaaatta
      601 aaatttaagg caatcaatta cttggaagag tatgaaagca tttagtttat aggaattcta
      661 ggcctagaat tacatatgta tttatgaaga taggggtgtt cgatagttag tattgttctg
      721 ttctgaaaga tttgagctgt cagttgtata gaaagtgttc gaattttttt taagtgatta
      781 aattagttaa ttgtatgagg ttctttatga tataatgttc ttaatgaatt ttcagaaagg
      841 aaaacctcaa attgttctac aaatttctac tcttcgacct cgaccacact cttcttgatt
      901 ttgatgctgc tgaggatgtg gctttgaccc aacttctaaa agaagaagga gttgcggata
      961 ttcaggctta taaagattat tacgttccta tgaacaaggc tctctggaaa gacttggagc
     1021 tgaagaaaat cagtaaacaa gagctggtta acacgcgctt ttctcgttta tttgctcatt
     1081 ttggacagga aaaagacggt agttttcttg cccagcgtta ccaattttac ctcgcccagc
     1141 agggacaaac actatcgggc gctcatgatc tcttggacag cctcattgag cgtgattata
     1201 acttgtatgc tgcgacaaat ggcattactg ccattcagac aggacgtttg gctcaatctg
     1261 gtctagcacc ttatttcaat caagtcttta tctcagaaca gttgcaaact caaaagccgg
     1321 atgctctctt ttatgaaaag attggccagc aaattgctgg atttagtaaa gaaaagacgc
     1381 tgatgattgg agattctcta accgccgaca ttcaaggtgg caataatgcg gggattgata
     1441 ctatctggta taatcctcat cacctcgaaa atcacacaca agcccagccg acttacgaag
     1501 tctattctta ccaagacttg ctggattgtt tagataaaaa tattcttgaa aagatcacat
     1561 tttaaaggag acgagctaat gactacaaaa aagctaatat tactattgaa gagtacattg
     1621 aaatgtctga agttgatttt aatgaagctg ttaattatga atttacatct gacacttgtc
     1681 aattagcaaa tagtatttat caatctcttt ttaagttttt tgataagaaa aatttctctg
     1741 gcgatttaat ttttacttgg aaatctccat cattagtcaa agaaggggat tatattggga
     1801 gaagggattc acaagtagat aatcttagag taataggaaa tatatttccg aattatctta
     1861 ctaatcgaaa atatagcctc aatatgaatc gtaatggctg tatgggagat tttcctcatg
     1921 acttttttga tatataccta gatcatgtag caaaatatgc ctacgaacaa aaagttaata
     1981 atattaaaga gtattatcct ttaaaaagag cgattttaca ccaagagaat gcattgtatt
     2041 ttcgattttt ttctaatttt gacgactttt tagaaaaaaa ttatttaaag actatatggc
     2101 aagtttctaa agaaactcct ttttctgaaa tggattttaa tatgtttaaa aatatttcag
     2161 aaaagataat cttcgaaaga ggtagtaaaa tgcttaatga tttaaaaagt aattataaaa
     2221 aatagccagt ctttataaac tgtattcacg tattcaaatc gtatatagtc gatttattaa
     2281 atctttagat aaactgtaaa aagtggtttc catagccact ttttgctata atagaggcag
     2341 taaaaatgaa ggagtcggct atggaacact cgtcttggca tgctttgatt aaggcgcaat
     2401 tacctgaggg ttatttcggg aaaatcaatc agtttatgga gcaggtctat tctcagggga
     2461 ttatttatcc acccaaggaa aaggtttttc aggctctctt gacaacactg cttgaagaag
     2521 ttaaggtggt aattctaggg caagacccct atcacggacc aggtcaagcg cagggcttga
     2581 gtttttctgt acctgactct atcccagctc caccatcctt gcaaaatatc ttgaaagaat
     2641 tgtcagatga tatcggagtt aagaaatctc atgatttgac agcttgggct gagcaaggag
     2701 tcttgcttct taatgcttgt ttgactgttc ctgctggaca ggccaatggt catgctggtc
     2761 aaatctggga gccttttact gatgctgtga ttcaggtggt caatcatcta gatagaccag
     2821 tcgtttttgt actctgggga gcttatgcac gtaagaagaa ggccttagtt accaatcctc
     2881 atcacttgat tatcgaatca gcccatccaa gtcctttgtc ggtttataga ggattttggg
     2941 gttccaagcc cttttccaag gccaatgcat tcttaaaaga gacaggacaa gagccaatcg
     3001 attggcttag ataaggagaa aatatgcctc agttagcgac gatttgctac attgataatg
     3061 ggaaagaact gctcatgctc catcgtaata agaaacccaa tgatgtccat gaagggaaat
     3121 ggattggtgt gggtggtaag ctagagagag gagagacgcc ccaggaatgc gcggcgcgtg
     3181 aaatccttga agaaacaggg ctcaaagcca agccagttct aaaaggtgtc atcacttttc
     3241 ctgaatttac accagattta gactggtaca cctatgtttt taaggtgacg gagtttgagg
     3301 gcgacttgat tgactgcaat gaggggacgc tagaatgggt tccctatgac gaggttttga
     3361 gcaagccgac ttgggaaggt gaccacacct ttgttgagtg gcttttagag gataaaccct
     3421 tcttttcagc caagtttgtt tatgatgggg ataaattgtt ggatacccaa gttgatttct
     3481 atgaataaag gagaaagcag atgctactaa tcaaaaatgg tcgtgtaatg gatcctaagt
     3541 ctggtttgga tcaagtttgt gatgtcttag ttcaagatgg gaaaattatc aaaattgcgc
     3601 ctgagatcac ggaagaagga gcagaaacga ttgatgctac tggtcttgtg gttgctcctg
     3661 gtttggtcga tattcatgtg catttccgtg aacctggtca aacacataaa gaagacattc
     3721 atactggtgc cctggcagcc gctgcaggtg gttttactac tgtcgtcatg atggctaata
     3781 ccagtccaac catttcagac gtggagactt tgcaagcagt tctccagtca gctgccaaag
     3841 agaagattaa tgtcaagaca gttgcgacca ttactaaaaa ctttaatggt aaaaacttga
     3901 ctgactttaa ggcgctctta gaagctggtg cggttggttt ctctgatgac ggtattccgc
     3961 ttgagagcag taagattgtc aaggaagcca tggaggaagc caaaaaactc aataccttta
     4021 tcagccttca tgaggaagat ccaggtttga acggtgttct tggctttaat gaaaatattg
     4081 ctagagaaca tttccatatc tgcggtgcta ctggggtggc tgagtacgct atgatggcgc
     4141 gtgatgtcat gattgcctat gcaactaaag cccatgttca catccagcat ttgtctaagg
     4201 aagaaagtgt taaagtagtg gagtttgctc aggggttagg tgcagaagtc acagcagaag
     4261 tagcgccaca gcatttctct aagacagaag cacttctttt aacacaaggt agcaatgcta
     4321 agatgaatcc accgcttcgt ttggaatcag accgtcgtgc cgttatcgaa ggtctcaaat
     4381 caggtgtcat cacagttatt gcgactgacc acgcgcctca tcatgtagat gaaaaaaatg
     4441 ttgaggatat taccaaagcg ccatctggta tgactggctt agaaacatcc ctgtctctcg
     4501 gcttgaccta tttagtagaa gctggtgagt tgagcttgat ggaattactt gaaaaaatga
     4561 catacaaccc agccaagctt tacaactttg aagcaggtta cttggctgag aatggtccag
     4621 cagatatcac tatttttgat gccaaggctg accgccttgt ggactcccat tttgcttcca
     4681 aagcagctaa ttcaccattc atcggtgaaa ccttaaaagg gcaggttaaa tataccatct
     4741 gtaagggaca aatcgtctat caagcttgat aggagttaga atatgaatta ttttcgaaaa
     4801 cgtagggaga gacaagcgaa atctaatagt ggaatttact gtcccgcagc taatcgttcc
     4861 aaaattcaat atgaaactaa agaaaaagct gaccatgcgg ttcaatatga taacggaggt
     4921 ttagttagaa gctattattg tcgtacatgt gcgtgttggc atactacttc aaaatctaat
     4981 aaacctccgc taagtttgaa aaactatggt ctgaaactct ttggtttaga taaatcagaa
     5041 tagaaagaga agtatgtata agacaaagtg tttacgagag aagttagtat tatttttaaa
     5101 aattttcttc ccaatcctga tctaccaatt tgccaattat tctgcctctt ttgttgatac
     5161 tgcaatgaca ggtcaataca acactatgga cttggctggt gtatctatgg caaccagtat
     5221 ctggaatcct ttctttacat ttctaacagg gattgtgtca gccttggtgc ctatcattgg
     5281 tcaccatctt ggtcgaggca aaaaggaaga agttgcgtct gatttttacc aatttattta
     5341 tttggccttg ggcctatctg tggtcttgct ggggatggta cttttcttgg caccaacaat
     5401 cttgaatcat attgggttag aagcagcagt agcggcagta gcggttcgct atctttggtt
     5461 tttatctatc gggattatcc ccttgttgct ctttagcgtc attcgttcct tgctggattc
     5521 gctgggcttg accaaactgt ccatgtacct catgcttttg ttactccctc tcaatagcgg
     5581 atttaactat ctcttgattt acggtgcctt tggtgttcca gaactgggag gggctggtgc
     5641 tggtttagga acatccttgg cctactgggt cttgcttggg atttctgttc tggttttatt
     5701 taaacaggag aagctcaaag ccttacacct tgagaaacga attccactta atatggataa
     5761 aattaaggaa ggagttcgtt taggtctgcc tattggggga actgtcttcg cggaagtggc
     5821 tatcttttca gtggttggct tgattatggc taagttttcg tccttgatta tagctagtca
     5881 ccagtcagct atgaactttt caagtcttat gtacgccttt cctatgagta tctcatcggc
     5941 tatggctatt gtcgtttcct atgaagtggg agccaagcga tttgatgatg cgaaaaccta
     6001 tattggtcta ggaagatgga ctgccctcat ttttgcggcc ttcaccttaa ccttccttta
     6061 catttttagg ggaaatgtgg ccagtcttta tggtaacgac ccaaaattta tcgatttgac
     6121 agcgcgtttt ttaacttata gtcttttctt ccagttagca gatacctttg cggcgccgct
     6181 tcagggaatt ttgcgggggt ataaggatac agttattcct ttttaccttg gtttgcttgg
     6241 ttattggggc gtagcaatcc ctgtggctac gctatttgat tccctaacag attttggagc
     6301 ttattcttac tggatcggct tgattattag tttgattgtg agtggggcgc tctaccgttg
     6361 gcgtttaact gtgattatga agagatttga atctttagca aaatccaaac gctaaaaagt
     6421 ttgtgaaaaa atattcttga taagaaaatc ccttgttttc acggggtttt cttttttatt
     6481 ttgtgaaatt tcttttagaa aaactttgac agtatttgcc aaaaacggta aaatagtaag
     6541 gtaagaagaa agttagaaag gcaagaagat gtcaacttta gataaaaatc ttttgctaga
     6601 aatgttccgt aagatggaag aaatccgtcg catggactta aaaattgcac aattagtaaa
     6661 gaaagggaaa gtgccaggaa tgacgcactt ttctgttggt gaagaggcag ctaacgtggg
     6721 ggctatgtta gctctcaatc cagatgatct gattacctca aaccaccgtg gacacgggca
     6781 agctattgct aaagggatcg acctcaacgg aatgatggct gaaatccttg gtaaatacac
     6841 tggaacctgt aaagggaaag gtggatctat gcatatcgct gaccttgatg ctgggaacct
     6901 tggtgccaat ggtatcgtag gtggtggtat ggggatcgct gtcggtgcag ccctcagtca
     6961 gcaaatgcaa aataccggta aaatcgttgt ctgcttcttt ggagatggtg cgaccaatga
     7021 aggtgttttc cacgaagcag tgaacatggc ttctatctgg aacctgccag tcattttcta
     7081 ttgcattaac aacggttacg gtatctctgc ggatatcaag aaaatgacca atatagaaca
     7141 tatccatcaa cgtagcgccg cttatggaat tcctggaatg ttcatcgaag acggtaacaa
     7201 tgtcatcgat gtctatgaag gatttcagaa agctgtagac catgttcgca gtggcaatgg
     7261 tccagtcttg attgaaagtg taacttatcg ctggctcggt cactcatcat ctgaccctgg
     7321 taaatatcgt acgcgtgaag aagtggaatt gtggaaacaa aaagatccaa tcgaaaacct
     7381 ccgcaattac cttattgaaa ataacattgc aagtgccgaa gaattggaag aaatccaagc
     7441 gcaagtaaag gaagcagtag aagcttctgt taaatttgca gaggaaagcc cattcccacc
     7501 gcttgaatca gcctttgaag atatttacgc agactaagga gaaagagata aaatggaaac
     7561 aaaaacaatg tccttccgtg acaccattat ccttgctatg tctgaggaaa tgcgtcgcga
     7621 tgaaaatgtg ttcttgatgg gagaagacgt cggtgtcttc ggaggagact tcggtacttc
     7681 tgttggaatg cttgaagaat ttggtccaga acgcgtccgt gactgtccga tttctgaagc
     7741 tgccatctct ggagcagcag caggagcagc catgacagga cttcgtccaa tcgtcgatat
     7801 gaccttcatg gacttctctg ttattgccat ggacaatatc gtcaaccaag ctgctaaaac
     7861 acgttacatg tttggtggta aaggtcaggt tccaatgact gttcgttgtg cagctggtaa
     7921 cggagttggc tctgccgccc agcactcaca atctctagag tcttggttta ctcacattcc
     7981 aggacttaag gttgtggcac ctggaacacc tgcggacatg aaaggcctgc ttaagtcttc
     8041 tatccgtgat aaaaacccag ttattatact tgagtacaag tcagaattta accaaaaagg
     8101 ggaagtgcca gttgatccag actacacaat tccacttggg gttggtgaaa tcaaacgcca
     8161 aggaacggat gtaacagttg ttacttatgg aaaaatgctt cgccgtgtgg ttcaagctgc
     8221 tgaagaatta gcagaagaag gaatttcagt tgaaattgtt gacccacgta cccttgttcc
     8281 gcttgataag gatatcatca taaactcagt gaagaagact ggtaaggttg ttctggtcaa
     8341 cgatgcccac aaaacaagtg gctatatcag tgaaatttca gctattattt cagaatcaga
     8401 agcatttgac tatctagatg caccaatccg ccgttgtgca ggagaagatg tgccgatgcc
     8461 ttatgcacaa aacctagaaa atgcaatgat tccaacagtt gaaagtatca aagatgcaat
     8521 ccgaaaaact tataacaaag aatagagtta cataagataa gttatgtaaa taaaataaag
     8581 ctgacgagaa actttgtatc ttttaggtgc agagctcttc gtcattaata tcttatattc
     8641 gagcacgggc taaaagctcg gaaaaaagat aaatctcctt ggcttcatcg aagccatcgt
     8701 cgatttccta tttttcagtc gcttttgacg ccctttgtat cataaataga attggagagg
     8761 tcatggctga tgacaagcta agagcgactc ctgcagctag aaagttagcg gatgatttag
     8821 ggataaacct ctacgacgtt tctggctcag gtgcaaacgg tcgtgtccac aaagaagacg
     8881 tggaaactta taaagacaca aacgtggttc gcatttcgcc acttgcaaaa cgaattgccc
     8941 tcgaacataa cattgcttgg caggaaatcc aaggaaccgg tcatcgtggt aaaatcatga
     9001 agaaggatgt tttggccctg cttcctgaaa atatcgaaaa cgatagcatc aaatctcctg
     9061 ctcagattga aaaagtggaa gaagtccctg ataacgtaac accatatggt gaaatcgagc
     9121 gtattccaat gacaccaatg cgtaaggtta ttgcccaacg catggttgaa tcttacttga
     9181 ctgcgccaac cttcaccctc aactatgaag ttgatatgac tgaaatgttg gctcttcgta
     9241 agaaggttct tgagccaatc atggaagcaa ctgggaagaa gactactgta acagaccttc
     9301 tttcgcttgc agttgttaag actcttatga aacacccata catcaacgct tcattgacag
     9361 aagatggcaa gactattatc actcacaact atgtcaatct tgccatggca gttgggatgg
     9421 ataatggatt gatgacacct gttgtttaca atgctgagaa gatgagtctt tcagaactag
     9481 ttgtagcctt taaggatgtt attggccgta ccttggatgg taaattggct ccaagtgagt
     9541 tacaaaattc aacattcaca atcagtaatt tgggaatgtt tggtgttcag tcctttggcc
     9601 cgattattaa ccaacccaac tcagctatcc ttggtgtcag ttcgacaatc gagaagccag
     9661 ttgtcgtcaa tggtgaaatt gtgattcgcc caatcatgag tttaggatta accattgacc
     9721 accgtgtcgt agatggtatg gctggtgcta agtttatgaa ggacttgaaa gagttgattg
     9781 aaaccccaat ctcaatgttg atttaagagc aagagtattc atttttaaga tatagataaa
     9841 ggaaggaaga catggcctta gaaataatta tgccaaaagc cggcgtggat atgacagaag
     9901 gacaaatcgt ccaatggaat aaaaaagtcg gagaatttgt aaaagaagga gaaatccttt
     9961 tggaaatcat gactgataaa gtcagcatgg aattggaagc cgaagaagat gggtacttga
    10021 ttgccattct caaaggagat ggtgaaactg tccctgtaac ggaaattatc ggttaccttg
    10081 gtgaagaaag ggaaaacatc ccaacagctg gagcagcatc gccagaagct agccctgtac
    10141 ctgtagcaag tacctcaaac gatgatggta agagcgatga tgcttttgat atcgttgtga
    10201 ttggtggagg tcctgctggt tatgttgcag ccattaaagc tgcccaactc ggcggtaagg
    10261 ttgcccttgt tgagaaatct gaacttggtg gaacctgctt aaaccgtgga tgtattccaa
    10321 ccaagaccta ccttcataac gctgaaatta ttgaaaatat cggtcatgct gcaaatcgtg
    10381 gtatcgtgat tgaaaatcct aacttcactg ttgatatgga aaaactttta gaaactaaat
    10441 ctaaagttgt taatactctt gttggtggtg ttgcaggact tcttcgtagc tacggagtta
    10501 ctgttcataa aggaattggt acaatcacta aagacaagaa cgtcttggta aatggttctg
    10561 aattgcttga aaccaagaaa atcattcttg ctggtggttc aaaagtcagc aagatcaacg
    10621 tccctggtat gcaatctcca cttgtcatga ctagtgatga cattcttgaa atgaacgaag
    10681 tgccagaaag ccttgttatc atcggtggtg gagttgtcgg tatcgaactc ggtcaggcct
    10741 tcatgacatt tggttcaaaa gtgactgtta tcgaaatgat ggaccgtata gttccagcta
    10801 tggatgcgga agtttctaag aaccttcgct tgatccttga gcgtaaagga atgaccatct
    10861 tgactggtac taaactgcaa gaaatcattg aggaaaatgg tcaacttcgt atcaaggttg
    10921 aaggaaaaga cgatatcatc gcaagcaaag ctcttctttc aattggtcgt atgccagacc
    10981 ttgaaggtat tggagaggtt gagtttgaat tggatcgtgg ttgtatcaag gtcaacgaat
    11041 acatggaaac ttcagttcca ggcatttatg caccaggtga catcaacggt actaagatgt
    11101 tggctcacgc agctttccgc atgggtgaag tttccgctga aaatgccctt aaaggaaatc
    11161 atgcagttgc caaattgaat ttgactcctg cagccatcta cactctccct gaagtagcag
    11221 cagtaggttt gacagaagaa caagcccgtg agaaatacga tgttgccatc ggtaagttta
    11281 actttgctgc taacggtcgt gctattgcat ctgacgcagc tcaaggtttc gtaaaagtta
    11341 tcgctgataa gaaatacgga gaaatcattg gtgtacacat tattggtcct gcagccgcag
    11401 aattaataaa cgaagcatca agcatcatcg aaatggaaat cactgttgag gaaatgctga
    11461 agaccatcca cggacaccca acttactctg aagtgatgta tgaagcgttt gcagatgttc
    11521 taggaatggc catccattca cctaagaaaa aataaaacga agatagatta gactggaagg
    11581 ttatttccag tctctatcat ataaagtaaa gggatatcat gaaatacatt atcaatcatt
    11641 caaatgacac tgcttttaat attgccttgg aagaatacgc ctttaaacac cttttggatg
    11701 aggatcaaat cttcctactt tggattaaca agccatctat cattgttggt cgtcaccaga
    11761 acactatcga agaaatcaac cgtgattatg ttcgcgaaaa tggtattgag gtagttcgcc
    11821 gtattagtgg tggtggagct gtttaccacg atttaaataa cctcaactac acgatcatct
    11881 ccaaagaaga tgaaaataag gcatttgact tcaagagctt ctcaactccg gttatcaata
    11941 ccctggctca actcggggtt aaagctgagt tcacaggtcg taatgacctt gagattgatg
    12001 gtaagaagtt ctgtggcaat gcccaagcct atatcaacgg ccgtatcatg caccacggtt
    12061 gcttgctctt tgacgttgat ttgtcagtcc tcgcaaatgc ccttaaggtt tcaaaagata
    12121 aatttgaatc aaaaggtgtg aaatccgtcc gtgcccgtgt aaccaatatt atcaatgaat
    12181 taccaaaaaa aatcacagtc gaaaaattcc gtgatttact cttggaatac atgaaaaaag
    12241 agtacccaga gatgactgaa tacgtctttt cagaagaaga attggccgaa atcaatcaca
    12301 tcaaggatac taagtttgga acttgggact ggaactatgg taaatcacct gaatttaacg
    12361 tccgtcgtgg aaccaaattc actagtggta aggttgaagt ctttgctaac gttactgaat
    12421 caaaaatcca agacatcaag atttatggtg acttctttgg tattgaagac gttgctgcag
    12481 tagaagatgt ccttcgtggg gtgaaatacg aacgcgaaga tgttcttaag gcgctaaaaa
    12541 ccattgatat cacacgctac ttcgctggta ttagccgtga agaaatcgct gaagcggtag
    12601 ttggataaat caaaaaaaga agttggccaa cttctttttt tgatatttat ttacataatt
    12661 taaaatacgt gaaatcataa actatctaga gcattctttt gttcatcatt aacaatatgg
    12721 gtatagaggt cagtgacttg tgtgctggca tgtcctagtt ggtgactgac taaaacttgt
    12781 gatttagtcg catcatagag cctagttgct agcgtatggc gcagtttatg gggtgttaca
    12841 cgtactttaa aatcctctga gtatttagca accattttct caacgctaga agcatcgata
    12901 cgattaggaa cacctctgta gagagttaaa aaaagggctg tatctgtttt ttccgtttta
    12961 tagcgttgat tccgaatggc cagataattc tctaaataag gtttagcaaa agcagcgaca
    13021 ttgactgagt cacgtttgcc accttttcga gtaacatcaa taaccatcat ttttagattg
    13081 agatctctta gatctagatt aacagcttca gataagcgaa caccagatgc caagagaagg
    13141 gcaataatgg ctaaatctcg ttctttattt ttgttgaatg atgagagagc tcgatttgaa
    13201 agctgttgtg ggtgctcttg atcgatataa gttagaaaac cttctgtttc atcacctaga
    13261 aagagttttt gcttgatatt ttcagctctg gcagcaaggg tttctttctt tttcttggtt
    13321 gaaacttttt tcattacatt acgatagaaa taaggttccc cctgatcgtt ttcaacctcc
    13381 tcggttagat acttgtaaag actagaaagt gctgataagg ttcgattgat agttgtctgt
    13441 gaaacacctt gttttgttgt attagcattc agcaagggac gttcacgtag ataaaggata
    13501 aaggattcca tgtctttctt agacatattt tccaaaactg ataaaggaat atcggatatt
    13561 ttatcagcgt ttgaaatacc agactccaaa acccagctga aaaatcggtc atattcctta
    13621 aggtattcgt acagggttgt aaaactgtag ggcacagcca gcttagattg gtagtattcc
    13681 agaacatacc agggcatgag ttgttttagt ttgtcgattc gttccagtaa aatctcacgt
    13741 ttcatgtatt ttctccataa gtaagtatac tagtagtata gcatagactt gtatattttt
    13801 caagaaaatt atagtttttc agaaagatgt tataggagtt tttcaaagtg ataaaaagac
    13861 ttagacctac aaacaaaaca gaataaggaa aataataaaa atctcaccca ctaaacttct
    13921 gttaatacta cataactgaa gagctatata aggagctatg agatacaaaa cacattgtat
    13981 aatagattga aactagaata gtacatctgg acttctaaaa cattgctaga aattgatttg
    14041 attgttctga ttgttttatc ctatttctat ttcatttcac tatataattg ctattgtttt
    14101 ctttttcatg attttaatct tactgtacag atttgataca gtaaactcct ttctagcttt
    14161 atactttatc atttctaact taattatagt cttattttta ataaagttca attatttata
    14221 tgtaaaattc ctcatcagta aaaaataacc gatctcattt ctgagaatcg gttttctaaa
    14281 taaatcaagc tgagtattta cataacataa attatgtaaa tactcatact acaacaacaa
    14341 atctttgact tttccaattt cactttttgg aataaccagg tgaatcatat cacccagata
    14401 cattctggtt gagccgttaa ctgtttggct cttgccatta tggacttgag ttgtgatgag
    14461 gacgttgtgt ggtaagttga gttcatgaac ttgtttccca gcaattttat cagaaactgg
    14521 tatttcgata agtgtaactt ctccttcgct agatacttct tctggaagca ttttttccag
    14581 catggcttca tagactggcg tacctttgag caaatccatg ataatataag aaacaagagt
    14641 gacaagacca agtggcatga ggttgcgaat atctcctacc atctcagtta cgaggatcat
    14701 agcggttaag ggagcttttg atatggctcc aaaatagcca ctcattccta gaatgacaaa
    14761 tatagggaat tgctcttgac tgacaagtcc aagattgaca cagataacac caactaagga
    14821 accaagcaaa gaaccaagag ctaaaatggg gaggaaaatt cctcctggca gtccacttcc
    14881 atagctaatc atactccaaa taaagcggat caaaaagtaa gctaataaaa cttggaaact
    14941 aaaattttgt tcagttaaag aaaggacaag ctgatttccg ccaccaatga tttgaggtaa
    15001 gaagattccg actggtatga taatgataaa agccaagatg ggataataag ccctatccaa
    15061 atggattttt tgaccaatca agtcataaac tcttccaacg tttaatacag ctttctcata
    15121 gagaaaacct gaaaatccaa ggaaaattcc catgacgaga tatatccaat actggtctag
    15181 ggtcatggga ggaatgttat ctggcatatc caatactggt gtcaaaccga acatgagtag
    15241 agacacaaag tttgctacga tgctggctgc tagagttgag acccagaaaa agcgcgaaaa
    15301 atggtgatag acttcttcta caacaaagag aagtgctgca ataggagcat taaaggctgc
    15361 ggctaaacct gctgcagctc cactggcaat caaggaacgt tcctctactg gactggattt
    15421 gagccacttg gcaattcctt taccaccaac tgctccaagt tgaatgctgg gtccctctcg
    15481 acccagcatg agtccactgg caatagcaag aatacctagc acatattttt tccaaagaat
    15541 gccccaccag ttgagggaca tgaggccttt cagttcggct tcgacttgag gaattcctga
    15601 gcctttaata tctttttctg accgtgttag tttggcactg agccaacaga tgagtatata
    15661 aaacaaaacc agtacaaaaa gattgcgcac taagtaccct tgatcttgat aaactccttg
    15721 tatcaggtgg aagccctttt caattaagaa acgaaaggat ccgacgataa ttccaacgat
    15781 gaggccgaca atgattcctc gaccaacttg ggatagtata gtgctggatg caaaggcgaa
    15841 ttctttctta gaacggacta tttccgactg ttcctccatg gtatttcctt tcaattaact
    15901 ttctttttta cctaaaacca gtgatttaac gggttcaaag ctggttcggt gaattggggt
    15961 aactcctagt tttgtgaggc cttccagatg tttagctgtg ccatatcctg cattagtagc
    16021 gaaatcatag ccagggaact gctgatcgta ttccttcatc aattcatcac gtgttacctt
    16081 ggctactata gatgctgctg cgatagagag ggagttggca tctcctttga taatggaggt
    16141 ttgtgaaatg ggcaagtcca gtttcatggc atcaatcaaa aggtgctctg gttgagggct
    16201 gagctgggag attgcttctt gcatggctag tttggttgct tcatagatat tgacttggtc
    16261 gatgacctga ttatctatga taccaattcc aatcgacaag gcttggtctt gaacgacttg
    16321 gaaaatctcc agatgtttct ttttaggaat tttcttgctg tcgttgagac ctttaatctt
    16381 acaattttta ggtaaaataa cggccgcagc gactacagga ccagcaagag gaccacgacc
    16441 aacctcatca atacctgcaa ttaaggtcaa tccttgctta taaagttctt tttcataaga
    16501 aagcatggat tccaagcgca aattttcatc taattcagct tgaatggctc ttttatgctt
    16561 gctgatttcc ttttgaactc ctgagcgatt atccttttca agatctaaaa aaatagggct
    16621 ttctaactcc ttgactgtca caaggagttc tttgatttct ttaatcgttg ccatcgaggc
    16681 cttccaatgt atctaaggta tagttaccga gtttgccatc acggacttcc ttcacgaaga
    16741 gactgtaaaa acggtcatag tcatcacgga aaccgagggc gcgggtcata tccataataa
    16801 tcacaggcgc ttcttcttca attttcattt gtttgaagcg ttcagccagc ttttctggat
    16861 aatgttcttt gaaataattg ataccaaaaa tggtaacctc atccatagga agcaactgat
    16921 ccttgatagc tccagtcaat gccaacttaa gtgcaacagt ttcatcctca aacttaggcc
    16981 agagaatccc cggtgtatcc aagatttcca gatctttatt ggttttaagc cattgttgac
    17041 cttttgtgac ccctggcttg tttccaacaa cagcaatctt tttaccagcc aaacggttca
    17101 tcagagttga tttaccagcg tttggaatcc caataatcat ggtacgcaag gtttcaatct
    17161 gaatcccacg ttctttctgg cgagcaatct tatcagccat gagcttcttg gccgcatctg
    17221 ttacaacttt tacagtcact tgctctttgg agttgatagc tagcgtctgg attccttgtg
    17281 attcaaaata ctgacgccat tccttggtca ttgctggatc agccaagtcg gccttgttta
    17341 aaatcaagag ttttggttta tcaccaacaa tcttggttaa cataggattt tgactagata
    17401 gaggtaagcg tgcatcaact aaaatcgtca caaaatcaac aaattttaaa ttctcctgca
    17461 cctgtcgacg agctttagac atgtgaccag gaaaccattg aatagtagcc attgagtttt
    17521 ttcctttcat agcaaggatt tagatcccct attttatttt actattgttt gaacatagag
    17581 agttttcaaa tgaatgatgt ccaaacagtt cttgttttat gacatagaaa tctactttat
    17641 attatatcat gccttcttta ttttgtccga tgatagcctt ttaccctata tagaaaaaag
    17701 ccatcaaatg atgacttttc agagggagca aaaaactgtt cccgtcatat tatgaaccaa
    17761 aagaactaaa ctaccaacag ctaataggcc taaagctgta agtgcagaag ttgatgaacc
    17821 tgttgcagga agagtttcct gctgataaat aagagcataa cttacttcat tatcagtagg
    17881 aattcctgct tgaatcttgg tttgttcttc atcacctttt taaactttat ttcctaatag
    17941 taaacactat tttttattct caaaaattga acttctaaaa tcatttgttt ggtcaagata
    18001 ggctttaaag attgctctct tcaacttatg aacggcactg tcgatttctg gatcataatt
    18061 actccaacgt ggaccttcag catccttgag aacagcttgg tccatgaggg cttgcaattc
    18121 atccacatta ttgatagtct ttgtgccata gcttggccat ggttttgtcg gatctttaaa
    18181 ggtcacctgc ttcaagtttc caaactgatc cactcgttct tggtacatag ctgtcttaaa
    18241 ttctgcccaa gttttatact taccgtcaaa tacctttttc aaaacaagct catcggtcac
    18301 caagccccgt tctttaccat aaagattgat agtttgccct tgttgtttag catcttcttc
    18361 gtattggttt gagatataag gtaccattcc atccttaaag cctttggcag ccaaaagttc
    18421 gtacgcaatc ctacgtccca taaggtcccc tggtgtgcct ttctcactgc tgagagctga
    18481 atagattggg gcaaagagtt tgatggtata gtagccattt cgttcatagt cgccagactg
    18541 gtactcacga gctgataaga tgttgtggtc aatcaaactt tcaaaactat tcaggtttcg
    18601 ggcctctgct tctgtcaatt ctttgactac gttagtggca taaacctcat ttccatctgc
    18661 tggatctttc acatacttgt tctcaatttt tctgagagcc gccattttct ggtaaacact
    18721 tagtttatta acgattgact gcccttcgag atattccatc atgtagataa ggtcaaacat
    18781 gttatggaca tagttctgaa gatctgctgc gttttggaat ctctctgtcg gatccaagac
    18841 ttgaagacgg gatccctctg tactatctga ttttgagtgt ttcaagatag aattaatggt
    18901 aatggttgcg tcacttggtt ggtcaggggc ttgcaataag ccttttgcga agaactctgg
    18961 tcccaagcca tttcttcgac catagccacc aaggtaaata tcctgatccg aatcatgggt
    19021 catttcatgg gtataagtaa tggctccatc cttgtccagc atgcggtaag acatatagta
    19081 gacgccgtcc cctgtagcgt aggctccatg ctggttgtgt actaccttat tgccaactgg
    19141 tccaaagaag ttcttcatag ctggattgga gctatcaaac ttagcctccg ctgtagcttt
    19201 tcctgatgtt gtgtcatccc caaacttata ggcatcataa aggagaatcg tacgatagag
    19261 tttttcgcgt gactgttcat ctaaaatacg ataccaataa tcgtagtgat ctcgctgacg
    19321 tttggctgtt tcacgcgcat tttcttcaac aaaatcattg agagccttgc ccgctttatg
    19381 gtcactactg cggtagcgat cataagctcc aaatcctaga ctagacatgg tcgagatgac
    19441 aaatacggat ctctctggca aggtcaggag aggcaagacc atattgcggt atttccatgt
    19501 ggcactcgtg atacgatcat aaacaccgat agaatacttg gtgccagcta acccttgctt
    19561 cgttttcacc tcttcgatag tggatttttc ttcgacaatg taagccttag tctctgattt
    19621 aaaccagtca ttattgcttg tatttggtaa aaagactttt cggtaatgtt ccagcgtgct
    19681 aaacaaatct gtcgttccat gttgactggc aagactgata ccataagtat cgacattatt
    19741 cttagctaga agattgttaa agccagattt acctaactca atcagagtat ctagtgggga
    19801 agtatttcct ttaccaaaga agtctggatg atacataact aggtctttga cattcacttg
    19861 accatagtta aagttatacc aacgttctaa ataagttaaa ccgagtagca aggcttcctt
    19921 attattcttg attttatcta caagatagcc tctggtgact gagttatcgc tagctagacc
    19981 ggcatctgct gacaaaagtt ttttcaagct gtctcccaga ttttgttttg ttttggagaa
    20041 ctggtcttct aaatagagct cggttaactt aacttctgga gaaataccaa gtgtctttct
    20101 gatagcatct gactggtaat caagcttctg caactcaggc aagacttcct taacaataga
    20161 gtctcgatca tataagaatt ggttaggagt gtagagaagt cccgtattac ctagattata
    20221 ttctgctaga ttggcaaaat cagtctggta cttgagatca aatttctcag atgaatggtc
    20281 attatagtga agtaagagtt tgtttgcagt ctgtttgttg gaaacaatat ctgtgattac
    20341 ttggtcatcc ttcatcataa ctgcagataa gagttctttt tggtataaga gactgttctc
    20401 cttaaccagg tttccgtatt tgacgatggt tgacttatta taaaaaggta acaatttttc
    20461 aatgttctta taaaccaaag cacggttagc ttgataatgg gctatcttag aaaaatcact
    20521 gtctttcttg ccacttgttg agaggggttc caccgttggt agagtgagag gatttatttc
    20581 tgcttttttg cttacaattt gagaagcatc tagcattgtt cctctttctt caaaggattc
    20641 cttgctgacg acctcatcct tctctaaagt gacattgaaa actctattag ccttactact
    20701 aaatgtatta gctaccttca ttcctgtata gtggtatcca gtgatggcat ttccgtttgt
    20761 tacattgaca tcgctaagaa cattggtcaa ttcaccagcg tgtttttcct caccagaagt
    20821 tcgatcccac aaatatcctg ccacaccagc gactttacca aagtgcttga cattattaat
    20881 atcaccttcc gcataactgt tctgtatatg cgcatcttgg tctactagac ctgcaagacc
    20941 accgacagtc tgatctgacc tattggtgtt tgacgaaatg gttactgtcg ctttggattt
    21001 agcaatagac gcattttttc ctgttaaatg accgaccaga ccgccaatat tgtaggcatc
    21061 agtcgtttca taggtattga caattcttcc cttgaaactg ctctctgcga tgcttgattg
    21121 gtctgcctta gccaacaaac caccgacacc acgttctcca gccagaacac catcaacatg
    21181 aacttgttta atctttgtgc cattcgtagc ttcatttgct agtgaaccaa tatcattttt
    21241 ccctgaaata gcaacatttt ttagactcag tttttctact gtagcaccac tcaagttttc
    21301 aaacagaggt tttttcaaat tatagatagc ataattcttg ccatcttttt caccgattaa
    21361 acgaccagta aaggtgtcct tgatatagga tctttcatca ggaccaagct ccacttcgtt
    21421 agcattcagg ctggccgcta aatgataggt tccagaggga ttttggttta tagctttgac
    21481 caggttgcta aaggaagtaa agtttgtatt ttcctcttta gccttcttat cgaggtagaa
    21541 ggtgaaattg tcgacatatt tattttctag ttcttgctgg agtttctcag ctttggctgt
    21601 gattttataa actgactgcc catttctttt ctcttcagtt attgaagcta ctggtatata
    21661 gacatcttta aatgctgaag attttacctt gacaaagtaa gtatccgtat tctctggaat
    21721 tccatccaat gaaacatgtt gtctgtaggt tccattcgtc tgactataca actcaatatc
    21781 agaaacattt cttaattcga gttttttctc tggttctgtt tgtccattct ctgatgttgt
    21841 attacctgaa ttttctggtt ttgttgcagt tccgtttttt tctggttgat ttgattcttc
    21901 aactggtggt tttgaatcac taggtttatt ggatacttct ccagtatttt cgttagctat
    21961 tttcccagag tttgtttgtg tttcttctgc aggttgaact ggtttttctg tttcttgatt
    22021 tgaggtacct tctactgtgc cttcatttgg atttactgga acttcttcta cagttttttc
    22081 tgaattttca tttttagagt cattatgttc tggtttattt gattctccaa ctgaggttgt
    22141 cgaatcacta ggattactgg acacttcccc agtattttcg ctagatgtat ctggtgatac
    22201 tttctctgaa ttcgttgttg attcttctgc aggttgaact ggattttctg cttcttgaat
    22261 tgaggttcct tctgtagtac cttcatttgg atttactggt gtttcttctg ttggttttac
    22321 tggaacttct tcagtttttt ctggaccttg ttctttggtc ttctcaaccg gagtttcagg
    22381 ttttacttgc tcaatattac ccttatattc tggcaatggg gctacctgct ctggttcacc
    22441 tttatcactt accacagtat ctggcgactc tggttgaacc tcagtctcac ctttgtcggt
    22501 cacaactgct tcgggtaatg tcggttgaac ttctggttcg cctttatcac ttactacagc
    22561 ttcgggcaac tcaggctgaa ttgcgggttc aacaatagat ccagactgta cgtccttatg
    22621 ttctacacca gtctcaggtt gttcctttat aacttgagtt tttttagtac ctttttcgac
    22681 tattcttgga ctaggcgcag tcgttgaagt tgaaacaatt tctcgcgaaa cttcttcctt
    22741 gtttacagag aatattctga cgatttcaac tttcttacct aatttacctt cttgttttac
    22801 tcttacagtt ccttcagcta aatcaggatt ttcttgaatt tcttcttgaa aatctatttt
    22861 tgtctctata gtttcctcac gatataagag ttcaggtttg ttcaattgac ctgataaaac
    22921 ttcatcctgt ggatttaatg tatttacccc agtcttttct tttggagaaa tcttctcctc
    22981 tttcttcgtt tctagattct tatgttcggc taattgttct tgagaatctg aagattgttt
    23041 ctcttctttt cttggattga ttaattcagt agagaaaggt ttttcaacta cttgaacttc
    23101 tgtcggctta gttgaagaaa caggtgtttg ttcctgaata gcttgtactg ttgatggatg
    23161 gtctacaaaa tttggtgtaa cattataatc caccttttgt tgttttgtag gagtggctgc
    23221 tgatttttct tgattactta cttcaaaatc agaagtcgtt tttccctctt tgatatatcc
    23281 aatataagtg taacctgaaa tctctttagg aagaggtaat ttttctccag aggtcaattc
    23341 atagtccgta ttgtaattta gcaaaagatg attttctaaa gcatggactg aaactaagac
    23401 accatttccc attcccgcaa ccaatactaa atgtaatacc gttttattct taaccttttt
    23461 cttggaaaca gcaaaaatta aaattcccag agcagctaag ctagcaccag caactagggc
    23521 ttgcctctca ttcttgcttc cagtatttgg taattccgcc agttgatttt gagaatttaa
    23581 cttataaaca agataataag tttcatcatc attctccacg tatgtcggaa tatcatagac
    23641 aagctgcttc ttttcttctg atgatagctc tgaatctgcc acatatttat agtgaactcc
    23701 cgcagtttct tgagcatcca cagatgaact agctaataca gacataaaaa ataaacttga
    23761 aatcgttgca gatacaagtc ctactgataa ttttctaaat gaaaaacgct cttgtttttc
    23821 accaaaatac ttttccatta ttcctccttg aaataaaatt tatatatgtt acaaagacct
    23881 ttattatatt agtgtattat ctattatcta tagaaaaggc agtatacctt aattatactc
    23941 ttaatttaca aaaaagtctt aaaattgaga tgcgctttca tactttgttt tatattattt
    24001 gggggtacaa taacacctac catgaaattt acacggtagg tgttactcat atcactaatc
    24061 gttctaaaaa tggtttgagg cagttgagga gaattccttc tatccagctt ccttgtgctg
    24121 atgagcgatg gtcttcctgc aggctttttt ttagaaaatc tcggacttgt tctggtgcga
    24181 tttcaaattc aaaggctttc attttataga aaaagtcgat gagatgatct gacaggtatt
    24241 cagttgaaaa gggtacttca ccactttttc gatattctaa taagagtcta gaaaatcgag
    24301 ctttttcttc aggaagctca cgaaaatagg aattgaggat ccaagtctgc ttctgttttc
    24361 tttcaattgg atcctgactg gcaattcgtt ggtctttttc cagctctttt tggtattgtt
    24421 tggccttgat agctcgttct gctctatttt taccaaaaag aattttttcc cacttgcgtt
    24481 cttcttgagt cagggtctct gtaaagccaa agtaatcttg ataagcacgc tctgcgggtc
    24541 ccatggctag aaccagattg tctgcatatt gcttggcgat tttatccctc ttcttgcgtt
    24601 ctttctctgc ctggatacgg agttcttgtt cgtagtcaat tttctccttg cctagcttga
    24661 caaggtagag ttggtcatcc gatttcccaa gtaaaaaggg tttgatacac ttttcaagga
    24721 cttcttccat ccgagccttt ttctttggtt ccgccttggt ccaacttcct ccctgaaaga
    24781 cttctaggaa aagctggtag tctctctcag gcgcaaattg attgccacga ttgggtttga
    24841 aaacaccttt ttcccagagc cattttagaa gtcgctcgtc aaagtcactt ttattgacct
    24901 tgattttttc ctttttctga gcttttctgg ttagattttc aacctttctg agcagttttt
    24961 cttcctcttc caattgctgg tcaaggggca atcgatgaaa atgacgaaca cagtcgctac
    25021 caattggaaa gaggcgttgg cctgtgacac cgttaaagag ttcataagcg tatttgatgg
    25081 catttcgaca gacacaattg ctacggccaa taccgttaaa aatcaaggaa acttcattcc
    25141 attccttggt agcttgttcc caagtatccg ctttcgaagc ctgtaaaact gcatcgtgca
    25201 gggattttct aactggaagt gtcatgaggt ctcct
//
LOCUS       DAVBHS010000011        24327 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVBHS010000011 DAVBHS010000000
VERSION     DAVBHS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24327
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.011"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..618)
                     /locus_tag="VQ651_000313"
     CDS             complement(<1..618)
                     /locus_tag="VQ651_000313"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET2753360.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEK"
     gene            complement(769..2079)
                     /gene="secY"
                     /locus_tag="VQ651_000314"
     CDS             complement(769..2079)
                     /gene="secY"
                     /locus_tag="VQ651_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET2753361.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(2092..2532)
                     /gene="rplO"
                     /locus_tag="VQ651_000315"
     CDS             complement(2092..2532)
                     /gene="rplO"
                     /locus_tag="VQ651_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET2753362.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(2677..2901)
                     /gene="rpmD"
                     /locus_tag="VQ651_000316"
     CDS             complement(2677..2901)
                     /gene="rpmD"
                     /locus_tag="VQ651_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET2753363.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(2873..3367)
                     /gene="rpsE"
                     /locus_tag="VQ651_000317"
     CDS             complement(2873..3367)
                     /gene="rpsE"
                     /locus_tag="VQ651_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET2753364.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(3385..3741)
                     /gene="rplR"
                     /locus_tag="VQ651_000318"
     CDS             complement(3385..3741)
                     /gene="rplR"
                     /locus_tag="VQ651_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET2753365.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(3825..4361)
                     /gene="rplF"
                     /locus_tag="VQ651_000319"
     CDS             complement(3825..4361)
                     /gene="rplF"
                     /locus_tag="VQ651_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET2753366.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(4553..4951)
                     /gene="rpsH"
                     /locus_tag="VQ651_000320"
     CDS             complement(4553..4951)
                     /gene="rpsH"
                     /locus_tag="VQ651_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET2753367.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(5236..5505)
                     /gene="rpsN"
                     /locus_tag="VQ651_000321"
     CDS             complement(5236..5505)
                     /gene="rpsN"
                     /locus_tag="VQ651_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HET2753368.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(5523..6065)
                     /gene="rplE"
                     /locus_tag="VQ651_000322"
     CDS             complement(5523..6065)
                     /gene="rplE"
                     /locus_tag="VQ651_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET2753369.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(6089..6394)
                     /gene="rplX"
                     /locus_tag="VQ651_000323"
     CDS             complement(6089..6394)
                     /gene="rplX"
                     /locus_tag="VQ651_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET2753370.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(6472..6840)
                     /gene="rplN"
                     /locus_tag="VQ651_000324"
     CDS             complement(6472..6840)
                     /gene="rplN"
                     /locus_tag="VQ651_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET2753371.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(6866..7126)
                     /gene="rpsQ"
                     /locus_tag="VQ651_000325"
     CDS             complement(6866..7126)
                     /gene="rpsQ"
                     /locus_tag="VQ651_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET2753372.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(7151..7357)
                     /locus_tag="VQ651_000326"
     CDS             complement(7151..7357)
                     /locus_tag="VQ651_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET2753373.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(7367..7780)
                     /gene="rplP"
                     /locus_tag="VQ651_000327"
     CDS             complement(7367..7780)
                     /gene="rplP"
                     /locus_tag="VQ651_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET2753374.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(7784..8437)
                     /gene="rpsC"
                     /locus_tag="VQ651_000328"
     CDS             complement(7784..8437)
                     /gene="rpsC"
                     /locus_tag="VQ651_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET2753375.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(8450..8794)
                     /gene="rplV"
                     /locus_tag="VQ651_000329"
     CDS             complement(8450..8794)
                     /gene="rplV"
                     /locus_tag="VQ651_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET2753376.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(8806..9087)
                     /gene="rpsS"
                     /locus_tag="VQ651_000330"
     CDS             complement(8806..9087)
                     /gene="rpsS"
                     /locus_tag="VQ651_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET2753377.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(9191..10024)
                     /gene="rplB"
                     /locus_tag="VQ651_000331"
     CDS             complement(9191..10024)
                     /gene="rplB"
                     /locus_tag="VQ651_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET2753378.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(10042..10338)
                     /locus_tag="VQ651_000332"
     CDS             complement(10042..10338)
                     /locus_tag="VQ651_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET2753379.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(10338..10961)
                     /gene="rplD"
                     /locus_tag="VQ651_000333"
     CDS             complement(10338..10961)
                     /gene="rplD"
                     /locus_tag="VQ651_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET2753380.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(10986..11612)
                     /gene="rplC"
                     /locus_tag="VQ651_000334"
     CDS             complement(10986..11612)
                     /gene="rplC"
                     /locus_tag="VQ651_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET2753381.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(11828..12136)
                     /gene="rpsJ"
                     /locus_tag="VQ651_000335"
     CDS             complement(11828..12136)
                     /gene="rpsJ"
                     /locus_tag="VQ651_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET2753382.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(12402..13019)
                     /locus_tag="VQ651_000336"
     CDS             complement(12402..13019)
                     /locus_tag="VQ651_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HET2753383.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(13016..13606)
                     /gene="nrdG"
                     /locus_tag="VQ651_000337"
     CDS             complement(13016..13606)
                     /gene="nrdG"
                     /locus_tag="VQ651_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HET2753384.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVAGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKEIYEQVKRE"
     gene            complement(13599..14105)
                     /locus_tag="VQ651_000338"
     CDS             complement(13599..14105)
                     /locus_tag="VQ651_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2753385.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDVEDFSYEEWL
                     EGNRNKEMGIWLPENRVPSIQFVLFGESGCALGFLNLRLRLNEGLLNYAGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            complement(14149..14289)
                     /locus_tag="VQ651_000339"
     CDS             complement(14149..14289)
                     /locus_tag="VQ651_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753386.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(14301..16508)
                     /gene="nrdD"
                     /locus_tag="VQ651_000340"
     CDS             complement(14301..16508)
                     /gene="nrdD"
                     /locus_tag="VQ651_000340"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HET2753387.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNCRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            complement(16628..18166)
                     /locus_tag="VQ651_000341"
     CDS             complement(16628..18166)
                     /locus_tag="VQ651_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET2753388.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            complement(18203..18346)
                     /locus_tag="VQ651_000342"
     CDS             complement(18203..18346)
                     /locus_tag="VQ651_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753389.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            complement(18424..19956)
                     /gene="cls"
                     /locus_tag="VQ651_000343"
     CDS             complement(18424..19956)
                     /gene="cls"
                     /locus_tag="VQ651_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HET2753390.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            complement(20102..20659)
                     /locus_tag="VQ651_000344"
     CDS             complement(20102..20659)
                     /locus_tag="VQ651_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HET2753391.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQPAQQNTNTANAGGNQNQAAPVQNQPVAQP
                     TDIDGTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKI
                     YTVNGNQIVVDDMDRDPSDQIVLTK"
     gene            complement(20656..21906)
                     /locus_tag="VQ651_000345"
     CDS             complement(20656..21906)
                     /locus_tag="VQ651_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET2753392.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRIQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFEMLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNFSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSCREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            complement(22114..22419)
                     /locus_tag="VQ651_000346"
     CDS             complement(22114..22419)
                     /locus_tag="VQ651_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HET2753393.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            complement(22435..22854)
                     /gene="ruvX"
                     /locus_tag="VQ651_000347"
     CDS             complement(22435..22854)
                     /gene="ruvX"
                     /locus_tag="VQ651_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET2753394.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPLDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            complement(22858..23124)
                     /locus_tag="VQ651_000348"
     CDS             complement(22858..23124)
                     /locus_tag="VQ651_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HET2753395.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            complement(23211..23780)
                     /locus_tag="VQ651_000349"
     CDS             complement(23211..23780)
                     /locus_tag="VQ651_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2753396.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            complement(23846..24244)
                     /gene="spx"
                     /locus_tag="VQ651_000350"
     CDS             complement(23846..24244)
                     /gene="spx"
                     /locus_tag="VQ651_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HET2753397.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
BASE COUNT         6740 a         5398 c         4560 g         7629 t
ORIGIN      
        1 tttttcaata tctgagaaga catcattgat atcttgatta ccttcgatgt catgaaccaa
       61 acctttggca cggtagtgag caatgattgg ttctccttga gcaatattaa catccaaacg
      121 acgttttact gtctcaggct tatcatcttc acgttggtag taatcttctt ctttatagtc
      181 aactggtggg ttaaagacct tgtggaaagt ttctccagtt acgcggtgga tgatacggcc
      241 actcaaacgt tccaagaggc tgtcagggtt cacttcaata ttgataatac cttctagttc
      301 aatgccaagt tcagccaatg ttttgtccaa ggcatgagct tgttcaattg tacgtgggta
      361 accatccaat aagaatcctg tttctttaat atcatcttgt gaaaggcgtt cttttacgat
      421 tccatttgta acttcgtcag gaaccaattc acccttgtca atatatgact tagcaagaac
      481 acccatttca gtttgatttg ccattgcagc gcggaacata tcacctgttg agatatgtgc
      541 aacatggaat tgttctacga tttttgctgc ttgagttccc ttacctgcac caggtaagcc
      601 cataatcaaa agattcatga tttgatctcc ttattttatt tttaaataga agcaaaaagt
      661 cttttcaccc ctatttaaaa acaaaataga agaggggaga tccaactctt acaaatgtca
      721 gagtttaaac ctccactccc tcagtattta ctgattcagt aaatactttt attctgttct
      781 gtccatgaaa ccaacatact tacgtttcaa taggtaacct tccaattgct tgattccttc
      841 gatacctgta gagataatga tcaagagact tgttccacca aaggcaacaa catcagaaag
      901 accaaataca tctttagctg caatcggtaa aatggaaatc acaccaagga agagggaacc
      961 aacagttgca agacgacgaa gaagtttaga catatattct tctgtacctt taccaggacg
     1021 aactccatgg atataggcac cactcttttg taggctctct gctgcttttt caggattaat
     1081 ctgtacaaac gtatagaaga atgtaaagag aataatcaac aaagcataca tggcaatacc
     1141 agttggagaa gtagttgcca acatctcttg tgctaccctt acccaagccc aatcatgacc
     1201 tgtggcactc aaaaactgaa gaatagccgc aggcgctgca gtaatcgaac tggcaaagat
     1261 aacagggata actccagcag ggtttacctt taacggaagg taagagctag atggagcacc
     1321 ttgtgcaacc ttagtatatt ggattggaat tttgtattct gcttgttgaa cataagttgt
     1381 aaagtaaata atcaacaata cagtaataat caaaatgatt acgaaaatga tagatgaagt
     1441 gatacggcta cttgggacgt tcacaaagta gtccacatag atgccctgaa tcatctctgg
     1501 aattgaggaa acaatcccgg caaagataat catggaaaca ccgtttccgt atcccttatc
     1561 tgtaatttgc tcacccaacc aagtgacaat catactacca gctgttaaga tgataccaat
     1621 cgtcagaaaa acttgtggag ttaaagcagt tttaatcaat tgagctccag ccaaggtatt
     1681 aaaaccagct gtaatcccga tagattgcac aaaagcgaga actagagcaa tataacgagt
     1741 agcttgattc aattttcttc gacctacttc cccttgttta ccccactcta caaacttggg
     1801 taaaatatcc atttgcaaga gttggacaac aatagaagcg gtgatatagg gactaactcc
     1861 tagggcaaaa atcgaaaagt tttttagggc attccccgac accaagctca acatgtttaa
     1921 gaaggataat ccacttaaag cattcaagct attggcattc acaccaggaa ctgtaatgct
     1981 agttccgata cgaaagacca aaacgataaa aattgtaaat aaaatttttg atcgaacctg
     2041 cttgacttta agagcttctc ttaataattt aaaaaacata ggtcacctct cttagatgac
     2101 ttctactgaa ccacctttag cagtgatagc ttcttcagct gatttagaga atttagctgc
     2161 tttcacagtc aatttcttag tcaactcacc gttaccaaga attttaatac ctgacttttc
     2221 agctttaaca attcctgctt cgataagaac aactggagtt acttcagcac catcttcaaa
     2281 gacgttcaat tggtcaaggt tcacaattgc gtattcttta gcgttgatgt tagtgaatcc
     2341 acgttttgga agacgacgga acaatggagt ttgtccacct tcaaaaccaa ggcgaactcc
     2401 gccaccgcta cgagcttttt gacctttttg accacgacca gatgttttac cgttacctga
     2461 tgaagtacca cgaccaacgc ggttacgtac tttacgagaa ccttctgcag gtttcaattc
     2521 atgaagtttc atttttattt tctcctcttt tgtaaaatgc tagcgccgat aagggagaaa
     2581 aggttgtctc ccccatcaac tcgcctatac gatgtcatca tagatgacta tatctagttt
     2641 taggggatgg tacagtgcac atcccctaaa acttcattag tttacttctt caactgttac
     2701 taagtgagat actgctgtaa tcataccacg gatagcagcg ttatcttctt taataacaga
     2761 gctgttcaat ttgccaagtc caagtgctac aacagtttta cgttgtgatg gaatgcgtcc
     2821 gattggagac ttagtcaaag taattttaat ttgagccatt ttatcccctt tcttatgcca
     2881 aatcagaaac tgaaatacca cgaagggcag caatttcttc agcgcgtttc aattgtttca
     2941 aaccttcaac agttgcacga acaatgttga ttggagtgtt agaaccaagt gatttagatg
     3001 taatatctgc cacacctgcc aattccacaa cggcacgaac tgcaccacca gcggcaactc
     3061 cagaaccttc tacagcaggt ttcaacaata ctttagctcc accgaattct gaaagaactt
     3121 cgtgtgggat tgttgttcca accataggaa cttcgatcaa gtttttctta gcatcatcta
     3181 ctgctttacg gattgcttct ggaacttctt gagctttacc agtaccaaat cctacgcgac
     3241 cattgtggtc accaacaaca acaagagctg cgaaacgaag acgacgtcca cctttaacaa
     3301 cttttgtaac acggttgaca gcaactacgc gttcttctaa ttcaactgca ttgtctttaa
     3361 atgccatttt ctagtgtcct cctattagaa tttcaatccg ttttcacgag ctgcatcagc
     3421 caaagctttc acacgtccgt gatatagata tccaccgcgg tcgaacacca cttctgaaat
     3481 acctttagcg tttgcacgtt ctgcaacgag tttaccgaca gcaacggctt gttcagtttt
     3541 agttcctttt gaaacttctt tatcaagagt tgaagcactt gcgagcgtta cacccgctac
     3601 gtcatcaatc acttgagcgt agatgcctgt attagaacgg aatacgttca aacgtgggcg
     3661 atcagcagtt ccagagagtt ttccgcgaac gcgacggtgg cgtttttggc ggagtttgtt
     3721 tttatctggt tttgaaatca cagttttcac ctctttagtt ttaaatcgtg tgctatgcac
     3781 aaagttggaa aataggttgg tggttgatga tcaaccactc aacattattt acctgtttta
     3841 ccttctttac ggcgaacgaa ttcaccaacg taacggatac ctttaccttt atatggttct
     3901 ggtgaacgaa ggctacgtac gtaagcagct gtttgaccaa ctacttcttt tgaaattccg
     3961 ctaacaacga ttgttgttgg gtttggaagt tcaaaagtaa ttccttctgg agcttcaact
     4021 tcgtctggat gagatttacc aacagccaaa acaagtttag atccttgaag ctgtgcacgg
     4081 taaccaaccc cacgcatttc aagttctttc ttgaatcctt ctgatacacc aacaaccatg
     4141 ttgttcaaaa gggcacgagt agttccgtgg atagttttca tttcttttga atcgtttgga
     4201 cggtgaagag ttatttcagt accttccaca cggatttcaa tatcttttga gaactcacga
     4261 gtaagttctc ctttaggtcc ttttacagtt acaacgttgt cattgttagc gagttcaaca
     4321 ccagcaggca acacgataac tttattacca atacgtgaca tgtttatttt ctcctgttaa
     4381 attgtcaggc cagaacggcc agttttcacg gggttcagat acttatttag ttcaagagct
     4441 gaactgaaca cgctctaaga actgtgcaaa atagataagt tggcttgttt gtaaacaaac
     4501 ttctccaact tcctaatttt gctttgttct ttacgagctt tgtatcttga ttttaccaaa
     4561 cgtaagcgat aacctcacca ccaacattct tttggcgtgc ttctttatca gtaagcaaac
     4621 cttcagaagt tgaaaggatg gcaattccaa gtccgttaag aacttttgga aggtcttcac
     4681 gttttttgta gacacgaagt cctggtttag aaacacgttt caagttagtg ataactttct
     4741 caccatttgg tccgtattta agaaatacac ggatgacgcc ttgtttgtca tcttcaatga
     4801 tttcaacgtt ttttacaaaa ccttcgcgtt taaggatttc agcaatccct tttttgatgt
     4861 ttgatgcagg tacttcaagt acttcgtgtt tagcttggtt agcattacga atacgagtta
     4921 ggaagtctgc gattgggtca gtcataacca ttttgttttt ctcctcttac tagtagtttg
     4981 caagttgcac ttgctagtta atacatgata caaagagcgt aacagcacga gcaaaaatag
     5041 acaatttaat gcagaagcaa tgctccaaag caaattggtc ttttttgcca aagctgtagc
     5101 tcgtgttcaa attggcaagc ccaattgaac ccgggctaaa ctctgtgtga aaaagataaa
     5161 ctttcctaga aactttagtt tcttcgtcaa gtttcctatt tttacttgga gttttgacgc
     5221 tcttgatatc ttaaattacc aagatgcttt tgttacacca ggaatttgac ctttatgcgc
     5281 aagttcgcga aaagcgatac gactcagacc aaatttgcgg taaactgaat gtgggcgccc
     5341 cgtaactcta caacgattat gtaaacgagt cggtgaggcg ttgcgaggta atttagataa
     5401 accttcgtag tcccctgccg cctttaatgc agcacgtttt tcagcataac ggtcaacaat
     5461 tttttggcgt ttagcctctc tagctaccat tgatttttta gccattagat ttacctccta
     5521 tattattttg caaaaggcat tccaaggcct gtaagcaatg cacgtgactc ttcgtcagtg
     5581 ttagcagttg ttacgataac gatgtcaaga ccacgagttt tgtcaacgtc atcgaagttg
     5641 atttctggga agattaattg ttctttcaca ccaagtgtgt agttcccgcg tccatcaaat
     5701 gattttgttg gaacaccgtg gaagtcacgt acacgtggaa gtgaaactga taccaattta
     5761 tccaagaatt cgtacatacg ttcaccacga agggtaactt ttgcaccgat cgcaacacct
     5821 tcacgaagac ggaagccggc gattgatttt ttagctttag tgataagtgg tttttgacct
     5881 gagataagtg ccaattcttc agcagctttt tcaaggcttt tagcgtttga tacagcttca
     5941 ccaacaccca tgttcaaaac aatcttatct actttaggca cagccatcac tgatgagtag
     6001 ttgaattgtt ctgtcaaagc aggaactact tcattaagat atttttcttt taaacgattt
     6061 gccattatac ttctcctttc cttcgtgatt aatcaagcac ttcgcctgat tttttgttgt
     6121 agcgaacttt tttaccgtct acaaatttgt aaccaacacg accagctaca ccatttttgt
     6181 ccaaaacttg aacgtttgat acgtggatag ctgcttcttt ctcgatgata ccaccttgag
     6241 gaagctcgtt agttggacgt tggtgtttct taacaatgtt aacaccttca acgataactt
     6301 tgtttacttt tggaagggca gtaaggacaa cagcttctgt tcccttatct ttaccagcga
     6361 ttacgcgaac tttgtcgcct ttttttacaa acattaggtt tctccttgat ttttcttacg
     6421 cccataaggg caccctagct tgaagctagg ggactagttt gttcctaaaa attaaagtac
     6481 ttctggagca agtgacacga tcttcatgaa gccaccttca cgcaattcac gtgcaactgg
     6541 gccaaagata cgtgttccgc gaggagtttt gtcttcacgg atgataactg ctgcgttttc
     6601 gtcaaatttg atgtatgaac catcagcacg acgagcacct gatttagtac gaacgataac
     6661 tgctttaaca acgtcacctt ttttaaccgc accaccagga gtagcttgtt ttacagatgc
     6721 cacgataaca tcaccgatgt ttgcaaattt acgtcctgaa ccaccaagaa ctttgatagt
     6781 caagatttcg cgagcaccgc tgttgtctgc gactttcaaa cgagtttctg tttgaatcat
     6841 ttcagttttc tcctttcagg tttgattaga tgatgaccgc ttcttcaaca acttctacaa
     6901 gacggaaacg ttttgtagct gaaagcgggc gagtttccat gatacgtacg atatcgcctt
     6961 ctttggcaac attgttttca tcatgagctt tgtatttttt agagtagtta atacgtttac
     7021 catagactgg gtggttacgt tttgtttcaa ctacaactgt gattgtcttg tccattttgt
     7081 cagatacaac acgtccaaca agaactttac gattattgcg ttccattgaa atttctcctt
     7141 ccctagtcta ttatttcgct tcagattgaa ctgttttgat acgagcgatt tgttttttaa
     7201 cttctttcaa gcgagctgtt tgttccaatt gaccagtagc agcttggaaa cgaagttcaa
     7261 acaattcttt tttcaattcg ttttcgcgct tcgcgagttc ttcttgagaa agaccacgaa
     7321 gttctttaac aaattctttt acttcattaa gtttcatgcc ttctccttat tctgcttcac
     7381 gttttacgaa tttacattta actggcaatt tgtggctagc aagtcgaagc gcttcacgtg
     7441 caatctcttc agatacacca gcgatttcga acatcacttt accacgttta actggtgcta
     7501 cccaaccttc aggtgcccct ttaccagatc ccatacgcac accgatagct ttagcagtgt
     7561 atgatttgtg tgggaagatt ttaatccaaa ctttaccacc acgtttcatg taacgagtca
     7621 tggcgatacg agcagcttcg atttggcggt tagtgatcca gtggctagtt gtagcttgaa
     7681 gaccgtattc accgaatgct acttcttttc caccttttgc ttcaccgcgc atttttccac
     7741 ggaactcacg acggtgttta acacgtttag gtactaacat tggttattta cctcctttag
     7801 tgtttttacg agctggaaga acttcaccac ggtagatcca tactttaaca ccaagtttac
     7861 cgtatgtagt atctgcttct tcccaagcgt aatcgatatc tgcacgaagt gtgtgaagcg
     7921 gaacagttcc ttcagagtat ccttcagcac gggcgatatc tgcaccgttc aaacgacctg
     7981 atacttgagt tttgattcct ttagctccag cacgcattgc acgttggatt gcttgttttt
     8041 gtgcacgacg gaaagcaaca cgttgctcca attgacgagc aattccttca cctacaaggt
     8101 gagcatccaa atcaggttgt ttgatttcga tgatgttgat gtgtacttgt tttccagtca
     8161 atttgttaag ttttgcacgg agtgcatcaa cgttagcacc acctttaccg ataaccatac
     8221 ctggtttagc agtgtgaagt gaaacgttaa ctttgtttac tgcgcgttcg atttcaatag
     8281 ttgaaactgc tgcgtcagca agttcttttt gaacgaattt acggattgca agatcttcat
     8341 gaaggtaatc cgcgtattct ttttcagcat accatttggc atcccaatca cggatgatgc
     8401 cgacacgcat accaattgga tgtacttttt gacccacgat tttacctcct tatttttctg
     8461 caacagctac agtgatgtga gctgtacgtt tgttgattgg tgaagctgaa cctttcgcac
     8521 gtggacggaa acgtttcata gttggtcctt cgtttgcgaa tgcttcagat actaccaaat
     8581 tagctttatc caaaccaaag ttgttttcag cgttagctac agctgagttc aaaactttca
     8641 agatgatttc agcagctttg tttggagtga atgtcaagat tgcgattgca tcggctacgc
     8701 ttttaccacg gatgttatca agaacaagac gtgatttacg aggtgaaaca cgtactgtac
     8761 gagccattgc tttagctgaa gtaatttctg ccatttatgt tctccttatt ttctacgtgt
     8821 tttcttgtcg tctgcagcgt gacctttgta agtacgagtt ggtgcaaatt caccaagttt
     8881 gtggcctacc atgtcttctt ggatgtaaac aggtacgtgt ttacgtccgt cataaactgc
     8941 aatagtgtaa ccaatgaaac ttgggaagat cgttgaacga cgtgaccaag ttttaataac
     9001 ttttttcttt tcgtcgttag cttgagcttc aacttttttc atcaaatgct catcgacgaa
     9061 aggtcctttt ttaagactgc gtcccatttt tatattttct cctttaaatg ttgtaccaca
     9121 gcggcttgca ctcctatgga gcgctaccga gctggcggat ttactagttg cttaagcgac
     9181 tagtttaata ttatttctcg ttgcgacgac gaacgataag tttgtcagat ttcgctttct
     9241 tgttacgagt tttaagacca agagcaggtt tgccccatgg agtagatggt gctttacgac
     9301 caactggtgc tttaccttca ccaccaccgt gtgggtgatc gttagggttc attacagaac
     9361 cacgaactgt tgggcggata cctttccaac ggctacgtcc tgctttacca aggtttacaa
     9421 gtccatgttg ttcgtttccg acaacaccaa ctgtagcacg gcaagttcca agaatcatac
     9481 gaacttcacc tgattgaaga cgaacaagaa catatttacc ttcagaaccc aatacttgag
     9541 cagatgcacc agcagcacgt accaattcac caccacgacc tggtttcaac tcgatgttgt
     9601 gaatcaaagt accaactggg atgttagcaa gtggaagagc gtttccgact ttgatatctg
     9661 cttctggacc tgaaacgata cgttgaccta cttcaagacc ttttggagcg atgatgtatg
     9721 ctttcacacc gtcagtgtag tgtacaagag cgatgtttgc agaacggttt ggatcgtact
     9781 cgattgtttt aacaactgct tcaacgttgt ctttattacg tttgaagtca accaaacggt
     9841 aaaaacgttt gtgtccacca ccttggtgac gaactgtgat acgaccgttg ttgttacgac
     9901 cagccttgct cttcaatgca acaagcaatg atttttcagg agtgcttgtt gtgatttcag
     9961 cgaaatccaa agaagtcata ttacggcgac cgtttgttgt tggtttataa acacgaattc
    10021 ccacgatatt tcctccttag attattcagc ttcagcagca aacaactcga ttgctttaga
    10081 atcagctgta agtgtgatga tagctttttt agttttgtta gtaaaaccag tgtaacgtcc
    10141 aacacgttta gcttttggtt ttacgttgat tgtgttaaca ttggcaactt taacaccttc
    10201 gaaagcagct tcaacagctt gcttgatcaa aagtttgtgt gcacgagtgt caacttcaaa
    10261 tacatatttt cctgcttcaa gttgagccat tgagctttca gtgatgacag gttttttgat
    10321 aacatcatac aaattcatta tgcaagaacc tcctcgattt tagagatagc tgcttgtgtg
    10381 acaagaagtt tgtcgctatt tgcgatgtca agaacacttg cagttgtagc agttgcaact
    10441 ttcacgtttg gaaggttacg agctgaaaga gctgcgaatt catttccttc ttcaaggata
    10501 acaagaactt tagaatcgat gctcaatgct gcaagaactt ttgcaaattc agcagttttt
    10561 ggagctgtaa atgaaagagc gtctacagct acgaatttgt tttcagcaac tttttcagag
    10621 taaactgatt taagagctag gcgacgaact ttttgtggaa gtttgtagcc gtatgaacgt
    10681 ggagttggtc cgaagacaac accaccacca cgccattgtg gtgagcggat agaaccttga
    10741 cgagcacgtc cagttccttt ttgacgccat ggtttgcgtc caccacctga tactgcagag
    10801 cggtttttaa cagcgtgtgt tccttgacga aggcttgcgc gttggctgat gattacatca
    10861 aacacaactg attcatttgg ttcgatacca aatactgcat cgctaagaac aacttggcca
    10921 gcttctttac cagtttggtc aaataatgtt acgtttgcca ttgtgactga tttccccttt
    10981 ctttattatt taccagcttt aactgctgat ttgatagtga taagagattt cttagcacct
    11041 ggtacgttac ctttgataag gataacgttc ttttctggaa caacttgtac aacttcaagg
    11101 ttttgaattg ttacgcggtc gccacccata cgccctgcaa ggtttttacc tttgaatacg
    11161 cggttaggtg caacaggtcc catagaacct ggacgacggt ggtaacgaga accgtgagcc
    11221 attggtccac gtgattgtcc gtggcgtttg ataacacctt ggaaaccttt acctttagaa
    11281 gtaccagtta cgtcaacaac gtctccagct gcgaatgttt caactgtaat ttcagcacca
    11341 acttccaagc cttcaacgtt tttgaattca cgaatgaagc gcttaggagc cgtgttagct
    11401 ttcgctacat gtcctttagc aggtttgttg ctcaatactt cgcgtttgtc atcgaaacca
    11461 acttggatag cgttgtatcc gtctgtttca acagttttaa cttgaagaac aacgtttgga
    11521 gttgcttcaa taactgttac agggatcaat tcgccagctt cagtgaagat ttgagtcatt
    11581 cccacttttt tccctaagat tccttttgtc atgagaaaat agttcctttt ctatattttt
    11641 attcaaaaag tttttaacga gcgtttttta tgctcaagtc taagatacaa agggcgtcaa
    11701 actcaacgtg aaaataggaa actgacacag tgtctagcag acgcgaggaa gtttatcttt
    11761 ttcacaccga gtttagcccg tgttcaatta gattgaaaca ccagcctctg ctcttttata
    11821 ttttagatta aagtttgatt tctacgttta caccacttgg aagatccaat ttcatcaagg
    11881 catcaactgt tttttgagtt gggttaacga tatcgatcaa acgtttgtgt gtacgcattt
    11941 caaattgttc gcgagagtct ttgtatttgt gagtcgcacg aatgattgtg tagaggctac
    12001 gttcagttgg aagtgggatt ggacccgcaa cttgtgcacc tgtacgagta gctgattcta
    12061 cgatttttgc agccgctgtg tcaagcgtac ggtgttcgta agctttcaaa cggatacgga
    12121 tttttttgtt tgccatcttt ttctcctttt cgtctattta agataatagg ctagctccac
    12181 aagaaaaccg acgcggtgtt gcgtggcaat gcaaccgagc gtgtcgcaac ctcttgcatc
    12241 aaaactaagg ctgtaattta cagcaccata atagaataac acaaatcccc tgcgattgca
    12301 agggatttgt taggtttttt cgttttttgg ggatgaaact gttttcctac tttttctttc
    12361 taaaattagg gattttcttc tatatttctt ctatacataa gttaagtcct cttgacatca
    12421 aatttatctt ctgattggtc cactaagata tccgccttag actcagtttc tttatagtat
    12481 cgcagatact gctcccgtct catctgatgg ctagccaata taaaggatgc atcgcgattt
    12541 ctcacagtcg tatctctagc aaggcgtcgc tttaattcgg tctcctcatc cgtgtagaaa
    12601 cagatggttt tttcaaagag ttccttgggt agaaagccaa cagacatccc ttcgacaatc
    12661 aaaattggtt tggctccaga caagacctca ctagccttcc aaggttcttc aattgtcaag
    12721 acatccatac ccgcctgcaa ggcaaggata tctctctgca aactctccag ttcatgcgcc
    12781 actggcagac tggctgtcac cttttgattc ggcgcgtcct tgggtactac caaatagcgt
    12841 ccggaggtga tatagggatc tgtttctagc aaatttactg tagtagaatc taaagcttgg
    12901 tacaattcct gtgcaaaggt tgatttacct gaagctccat gaccgtatat tcccagtgtc
    12961 ctgactttcc ccgtctcgat ctctgagact agttggtcta ctaagtcttt tttcttcatt
    13021 ctctcttcac ctgttcatag atttcttttc cgtcattgag cttgtcccaa atcactactt
    13081 gcccactttt gagcgatttt tgcacatcga taattcgttg gttagacgaa cctcgaaact
    13141 ggagcataag atttctctta gttcgatcat atcttccatc gacaagaatg tcaatcagtg
    13201 acaagagttc cagtttatct ggagtttcca acatcatttc ttcccaagtg tagcccgtcc
    13261 aggaccagat gtccttgtct ggcaattcct tccgaatccg cttaacaagt ggcaagagaa
    13321 tcccagtatt aagaaaaggc tcccctccta gtaaggttaa cccctgaaca taaggctggg
    13381 caagatctgc cataatctgt tcttccaact cagctgtata gggaatgcct gcattgaagg
    13441 accaggtcgc tacattataa cacccctcgc agtgaaacat acagcctgct acatagagag
    13501 agttacgcac gccttcgcca tcaacaaagt taaaggcctt gtagtcaatg atacgacctt
    13561 gactaagttc ctcgcttttc cattcttgtg gttttggatt attcattcgc tacctctatc
    13621 caataacgct cgactccatt gcgagcatcc tcaaatattc caccatttgc tagaatgact
    13681 gctctgctag caggattatt cacgctacag gtcaccagag ctttcttgat gttcttttcc
    13741 ttagcaactt gcaagccctg acggagagtc tcttttgcat aacccttgcc tctttcagat
    13801 ggacggatgg agtagccaat gtggccagca taattcagta acccctcatt cagtctcaat
    13861 cgcagattca aaaatcctag agcacaacct gattcaccaa acaatacgaa ctgtatcgat
    13921 ggaacgcgat tttcaggtag ccatattccc atttctttat tacggttacc ttctagccac
    13981 tcctcgtaag aaaaatcctc aacatcccaa aatcctccat catgggctga ttggctcttt
    14041 tcaaactctg tcatcatatc taaaacagct ttcttatccg ctaatcttgg tctgcgtaat
    14101 tccatcctta cctcccacta tgagaaaagc gagagctggc tctcactttt attttttctt
    14161 gttcttgttt aaaaattgtt ctctgaagct aagcaagcgt tctttcttac cagctccacc
    14221 tttagagtgt ttctcgtgat aacgggtcac ttgtgcgcgc cccttatcgt ctagttgata
    14281 ttttcccatt tcatttcttt ctaatttgtt acttgatgcc cagctatttt aatcgttgaa
    14341 ccattcatat gtttgacacg cgcagcgatt tccttgtgac gcccgttgac catcggtctt
    14401 gcttgaggat tacctaggta gccacaagtt cgcttcacca catctactgt tttagggtcg
    14461 ctattgccac agtttggaca agcaaaccct ctctcagttg gttcaaaatc cccttcaaag
    14521 tcacacttgt agcaacggtc aatcggagta ttggtgccta gatagcctac acggtcataa
    14581 gcataatccc agacagcttc caaggccttt ggattttgct gaaggactgg atactcacaa
    14641 taatggatga aaccacctga cgcacctgct tctggataga ctttctcaaa gtccaatttt
    14701 tcaaacggtg ttggattttt acgaacatcg tagtggaaag agttggtgta gtattccttg
    14761 tctgtgatat caggaataga gccaaacttg tctatatcta gtcggcagaa acggtctgtc
    14821 agactttcgg atggtgttga gtagatagag aaatggtagc catattggtc tgaccactct
    14881 tctacacggc gtttcatatc gtgaatgatg tctagcgtga attccttagc atctggatta
    14941 ctttcccagc tgttaccaaa gaaaactgtc gctacttcat acaagccgat atagcccagc
    15001 gaaacggtcg cacgacaatt cttaaagagc tggtcaacac tttcttcttt acctagacga
    15061 tggccaaaag caccgtactg ataaaggata ggagcattcg ctggtgtcgc ctctttagtg
    15121 cgttcgacac ggtaaacaag agcatcttct gcgatattca ttcgctcgtt gaagatttcc
    15181 cagaacttat tcatatcacc ttcagactca agagcaatac gaggcagatt aaccgtcaca
    15241 acacccagat tcatgcgacc tgaattgact tctacaccat tttcatcctt ccatccttgg
    15301 aggaaagaac ggcaacccat aggcaccttg aaggatcctg tcaagtcaac aatcttatcg
    15361 taagataaaa catctggata catccgtttg gttgcacact caagagccaa ctgcttgatg
    15421 tcgtagttgg gagttccttc ctctaaatta aggcctcttt tcagagtgaa aataagttta
    15481 ggaaagatag ccgtacggtg ttctgaacca agacccttga tgcgaatgtt taaaatagct
    15541 ttttgaattt ctcgttcaaa acgactggtt cccagaccaa aacctagcga agtaaaaggt
    15601 gtttgtccat ttgaagtgaa gagagtattg atttcatact caagagattg catggcatcg
    15661 tagatgtcct tttgcgtttt cttccaagcg taatcttccc gtttttcagg caatacccac
    15721 tcttctgcat ctttgagatg tttttgatag ttcttctctg cataaggcgc caaaatttca
    15781 tcgatacggt cagctgaaca gccaccgtac tggctagaag caacgttggc aatgatttga
    15841 gaaatctgtg ctgtcgcagt ctggatagac ttgggactct ctacctctgc atttccaatc
    15901 ttaaaaccat tttccaacat acccttaaaa tcaatcaaac agcagttggt cataggggta
    15961 tagggactgt agtccaaatc gtgatagtgg atatccccct tttggtgggc attggctacg
    16021 tgcttaggaa gcatttgcag tccgattgat ttcccaacaa tccctgctgt caaatcacgc
    16081 tgagtgttaa agacatcact gtctttatta gcattttcat tgacaactgt ctggtctttg
    16141 ttgagaagtt tatgaatact aaagttgata tccgtcgctt ttgagcgctc aaaatccctc
    16201 tgtgtccgat aagtaatata ctcctcagcc agcgcatatt ctttggcttc aaggagttca
    16261 tgttctacga tattttgaat ttcgtaaatc ttaattccct gtggaaagcg actatgaatt
    16321 tctgtaataa ttctctcagt cagatcattg aggcgttttt caaccagggg tgtcacatcc
    16381 ataaccttgt cagctgcctt gtggagagcc ttgtcaatct tgtccacatc aaatacaaca
    16441 cgtctcccat ctcgtttctc gacgaagaga gttggcaaaa ttgtaatttt ttcttctagt
    16501 gcaatcatat gcatgctctt ttctaaaatg ttctttctaa aaagtattat accactctaa
    16561 aaagaaaaat caatatcttg tgttaaaaat cctaaaattt ttaaaaatac acaagatatt
    16621 gatttggtta tttaatttta tagactttaa actctgagta atcagtcttt acttgttggt
    16681 aattttcttt taaaagtgtt tccacttcag accagactac caccttgtca tttaccacaa
    16741 tcaccttagg ttgtttttct ttcaagtcat taagtaactt attacgattt tcctcagttg
    16801 ctgtatagtg caacggggac aaaatcgccg atggcgacaa gcgctcactc ttacgataaa
    16861 gagtcgcagt atcatcccat gcatagatag catcttccgt atttgtctcc tcttttacca
    16921 aatcagcaag acgattacgt tcttgataag gtactggata aaggacaaac ttcatcaaaa
    16981 aaggaacaga caaaagatag acaaacccaa aaactagcag atatagattt cctttgaaga
    17041 aacgactcca taggctaact ggcgtttctc ttcggcttct tctagcacta cgccccctct
    17101 gttgactctt gatattggtt accaataaaa gtaggaaaac agggaagacg accatcaaat
    17161 aagaagcgtg cagaggctct tgtgaaaaga aaagtaaaat caaacctaaa attaccacaa
    17221 gacttgcagg aaccgaaatg acgtatagtt taaagggttt ggattgaaag agacctaaaa
    17281 agatacaaac cacaaatccc aaaccaaggg acaacaagcc ataaaaaaac atattctcta
    17341 aaattttaga agtaaaatca aagcgaatac tgtcaatagg ataacgaata ccactaatcg
    17401 catccccaaa acttcctgtt gcagcactat agtaggcagt tggataaaag acaagtgaaa
    17461 aacctaaagc cactgcaaga aactgataaa acccatgcgc aaagcgtcta tgcccaaggt
    17521 taaagaccaa caagcctaaa ctcactacag caataaacag gagtgatgat aagggagcaa
    17581 agaaaaatcc gcctgccaaa gctaacccaa tccgtacaaa tcccttatca tggcttggat
    17641 tgcttaggta agccgcaact aaactaaagg ctgcgaataa gaaaggaagc gctaaaagag
    17701 tcgcatagcc tccaccaaaa gcaagacctg taactagcat gtaaaaaata gtcaccagat
    17761 gtccagcttg gtctccttgc tctgtcaagg tgtccgctga tctaaaaagg aaaaatcctc
    17821 ctgctaccaa ggctaaccac tcaaagatgg caaagaaaaa tccgccctga ctcacgtaag
    17881 tcagcaaata ataaagcaaa ccttgacttc cataatagtc gctgtaaatc ttccctgtct
    17941 gatgaagcgc ccaacctgca taaaaatcct gcacttcttg tgcactcatt aagtcgagta
    18001 atagcggtac tcctagagtt atccccgtta caagcgtact ccatagtaaa attttcacca
    18061 aaggaagacg acttgattca cgatgatgcg attcttgctc gatttggtat tctagaggtt
    18121 cacgattctc cttatgaact tcttctactc taccatacac actcatatcg tttctcctgt
    18181 tcaatttatc tgtcttagta tatcaaaaag tcctaggatt gtcagcttgc gatggctgtt
    18241 tgagtgattt tttgcatagt ttcacaaagg tttcaatttc ccgaaagcgg tgtaaatgtg
    18301 tctgtttgaa ctcaatcata tcattcactt cttccttagt tagcatgaat tcccctcctc
    18361 tttctttcta ttcgtaaaag acaagaaaaa taggtcgggt ttgtatccta agttctagat
    18421 ttcttataag ataggggcga atagctgggc gatttctttt acaagctttt gataccagct
    18481 cgttttgata gaatgaggat agacttcctg agaatctgca aatatatttc gaaaatctcg
    18541 ggcgatttcc cttatagaag gagttttata gagtaagact gcattttcat aatggtgtac
    18601 caagctccgg tagtcgagat tgattgtccc caccaccgca aaatcttcgt ctaccaacat
    18661 ctgcttacta tgaataaaac ctggactata ctcataaatc cgaacaccag cagaaagaag
    18721 gtcgggataa gctcctctcg tgactaactg aatgaacttc ttatctggta tgtaaggggt
    18781 gataattcga acatcgaccc ctctcatagc tgcatttttg attgtctctg ttaaatcata
    18841 atctataatc aaataaggtg tcgtaatata gaccgattcc gttgcttgat tgattaaact
    18901 ctgataaact tttttcccta cctgcgctcg aaaaattggc ttgggtccac ttccgtatgg
    18961 aatggttaaa ccgtcactcg ggatagaatg attttctaaa tgatattgat caaaatcact
    19021 aatttctcct cgattaatgt accaagtggt caaaaataag cgtgtcaggg cttttactgc
    19081 tagtccgtct aagcgaattc cactatcctt ccaataacca aatctctcga cgtggttaat
    19141 gtactcatct gccagattga ccccaccagt ataggctatc tgaccatcaa caatcaatat
    19201 ttttctatga tctctgttat tataagccac tgtcaaacga ggaataactt tattgaattt
    19261 atgggcctcg atgcccagct gacgaagtcg atgtgcataa tctcctgtta aagtagccat
    19321 acagccgata tcatcataga gcatcttaac ctctacaccc tgagctacct tttgctctag
    19381 tatatctagt atgcgattcc acatcaaacc ttcttctata atgtaatatt ccaagaaaat
    19441 aaatttctca gcctttttaa gatcttctac catctttttc cacatagctt ctccgttagg
    19501 aaaaaatgta gaggcagttt gatcatagat gtcagcattg gtatccatac tcaataagga
    19561 cttgatgact ccataagctg ccttgtcact ttcttttaat ttctctttta gtagctggct
    19621 atttgcttct tggaaatgca tagagcctag cttcttcagt tgtttgattt cttttttgga
    19681 caatcgcctt tcaccaaaca tcagatagag taagggacca aatactggca caaaggctac
    19741 taacaaccag gttaccttat tctcaggagt cgtattacgg ttgactatcg aaatgattgt
    19801 gatgatactc agtagaatga ggacagtaat ccataaaatg ggagccatgc gtcctaaata
    19861 aagaaagaga ccaaaaacaa gacaaagttc aagtagcata atcgaaagac taaagccata
    19921 cttggacatc aataattgaa atttcctata tttcatatcc cctccttgat agtttgaatg
    19981 ctaaaaacaa gtaattgata ctagtataac gcaggtattc ggtagaaggc aagtatttat
    20041 gattattttc taggtaaaag taaaaaggct gaaaaatcca gccctttcta cttgcatagt
    20101 cttatttagt taaaacgatt tggtccgatg ggtctctatc catatcatct acgacgattt
    20161 gattaccgtt tacagtgtaa attttgacat catcgccaat aatcatgcgt tgatttgctg
    20221 aatcaaatgt aacctgtttg actttctgat ccccgtcaga ttcgagctca gtccatgtac
    20281 cagtcgttcc agttaccact aaagtgatac ggtctccgtc atcctgacca gtataagtcc
    20341 catcaatatc ggtcggttga gcaacaggtt ggttttgtac tggagccgct tgattttggt
    20401 tacctcctgc atttgcagta ttggtattct gttgagcagg ttgttgagca ggctgttgga
    20461 cctgctgctg agcaacaggc tgttgaacct gttgagctct ttgtgaacaa gctactaaga
    20521 gactaaaact tgctagagaa gcaagagaaa gtgtgaatct ttttaatttc atgatgaatt
    20581 tcctttctgc taccaattta gagaaatttt ctctaaccag caattcccct agtataacaa
    20641 gttcaaacaa gggggtcaat ttatctgctc acggtccagc aggtagcccc gtacttctga
    20701 gataaaatag agagaccctg taacgaacag caagtcttga gcgtctgccc tttcttcgaa
    20761 actgctgata aattctcggc aagaagaaac tatatcgtaa cctgtcacat ccctttcgtc
    20821 caaagccccc tgatagtcaa agccggtcac cttgagttcc acctgaggca atttttcagt
    20881 caggtaacct aacatccctt gataatcctt ccgtttcaag gctccaaaaa ggatttgagg
    20941 tcgatagcct tcctgctctt tttctttgat aaactcaacc aagcgagtca aggcagggag
    21001 gttatgagca ccatccaagt aaatctgtgg gcgaatacgc tccaagcgac cagcccaatg
    21061 ggtctgttcc aaggcctttc ttacagcctg ttcatcaaca gcttcctttc cttctcccat
    21121 aaaaagaaga aaagtttgca acgccaaggc tgcattctcc tgctgataga ctccttctaa
    21181 gcctatgttc agctgtgaaa aatttagtaa agagcttgaa aaatcaccat tcagcattaa
    21241 aaaatcttga cctgcctgat aaaggttaac agctaaagat tcggcttttt tctgacagac
    21301 aagcctagct tctggaggca atttcgcaat cactgccttc ttaccagcct tgaaaatacc
    21361 agctttctgc tctgcaattg cttctagact atcacccaag gtttcttgat ggtcaagccc
    21421 aatggaggtg atgacagcaa gctctccagt taccacattg gtcgtgtcaa gtaagccacc
    21481 aattcccact tctaataaaa ccaaatccac ctcttgctcc ctaaaataaa gaaaagcaac
    21541 caaggtcagc atttcaaaaa aggacaactg gtcaggagtt tgcagaagcg ttttctccat
    21601 ctccttgacc tgatcagcca aacggataaa gtctgcgtct gctataggtt gcccattaat
    21661 gcagattcgg tcattgatag agacgatatg aggggaggta aaggtcgcaa cttttttgcc
    21721 atgccccata aataattccc tcataaaagc aatggtagat cctttcccat tagtccctgt
    21781 tacgtggata atagggtaag actgctcagg atttcctaac aaatccaccg cttgctgtat
    21841 tcggcccaaa cctgatcgaa aattcaaacc aatccgacta tgaagccatt cttctacttc
    21901 aaacatacac atctccttga caaaagtcca atcaattatc gcattaaagt atggttacta
    21961 ataaaaacaa ggccaggatt ttcgtcccga cctcttacct ggttagctaa taactagcca
    22021 ctatgaatgt gaatatgggc taaaaacatc cactagatgt tccaactctt ccccatttct
    22081 gggagttggg ctgatacagt ctcccagact gtatcactcc tccataaagc tgttgaagac
    22141 ttcttcaatc atgttccatt cgtcttctga gtcttctggg attggttgca attcgccttc
    22201 tgttccatct tcattttcaa tgaatgagta agcttggatt tcaacttgtc cgtcttcgtc
    22261 ttcttctgcg ttaactggta ctagaagaac atagttttta ccaaattctt cttttccatc
    22321 aattgtcaaa aggatttcaa acaaggtttc atttccttgc tcatctacta gtgtgattag
    22381 ttcacgttct tcgtggtcgt ggttatgatc gtgtgacata gcctctcctt tatattaaaa
    22441 ttttctatct aaataatttt gtaaaatcag ctgagctgct aacttatcaa tgactttctt
    22501 gcgcttattg cgactgatat ctgcttgttc aatcaacatg cgctcagcag ccactgttgt
    22561 caagcgttca tcctgatagt ctaatggtaa accaaaaaac tcttctagct ttgctccgta
    22621 ggcttgacta gcttctacgc gcggtccact tgtattgttc atgtttttag gcaagcccac
    22681 tacaaatcgt tccaccttgt aagtatcaac caattcctta acgcggtcaa aaccaaattg
    22741 gccttgttct tcatttatct ggatgatttc aagcccttga gctgtaaaac caagcggatc
    22801 gctaatcgcc acccctaccg tttttgaacc gacgtccaat cccataattc tcataggtta
    22861 tagatcgact ccttgtcctt tgaggtagta gcgaaccaat tcctcaacga tttcatcacg
    22921 ctcatactta cggatttgat ttcgtgcatt attataacga ggaacgtagg cagggtctcc
    22981 actcaatacg taacctacga tttggttaat tgggttgtaa cccttatcgt tcaacgaagc
    23041 ataaacatct gtcaaagttt cgctaatttc ttttttattg gaatcgtcca atttaaaacg
    23101 tactgtttct tcagtaaatc ccattctaac accctctttc cttagaatag taccattata
    23161 gcataattcc ttaccttcta caattcaggc agtctattta tttggatttt ctattgttct
    23221 gtcgcgccat ttgccaatct atctgaaata tatttgcttg gttcattttt caaaagattt
    23281 tccaaaccaa tattcttcag atgttccaac tgggaagcct tcttgacatc cagaacttga
    23341 aaatcaaaac tagtcgttgt ttgaagttcc gttgcgctca atagttttgt ttcaagtttg
    23401 aaacctgcca atttacgagc ttcaatgata gacttatcct tctcctccgc ttcaagaaga
    23461 gctttttgag tttcctccac tccatgttgg tcaatataag tcttcaactc atcagagact
    23521 ggacgttttt gttggatagt tagactcaaa aaagtcttgt ccttgtatgt aatggtttgt
    23581 gtctgctggc taccatcatc tgacttgggc aagaccaaag tcttagttac aactgtattc
    23641 ttctcagcat tttcaataac tggcaatgcc gactgaagcg tatctttttc tgtttttgta
    23701 gctggtccag tttctttttt ctgtccgcaa ccaaccagga caaaaaggaa agctagacta
    23761 acaagaacta tttttttcat ttctttcttc tttctttttg aaattaaaat agaataagac
    23821 tgggaagtgc tcccagcctt gatgtttata gagctgcacg caaacgtgct tctgcatttt
    23881 ctacattacg gacagagcgt ggtaggaagg cacgaatatc gtcttccttg tagccaactt
    23941 gcaggcgttt ttcatctaca aggattgggc tctttaaaat tctcggtgtt tccataatca
    24001 gattgagaac ttcattgaca ctcaaatctt caatatccac tccaagggct ttggcatagc
    24061 gatttttaga cgaaacgatg ctggctattc cgttatctgt tttggttaga atatccagta
    24121 attcttctct cgtaattcct tctttaccaa ggttttgttc tttataactt aactggtggg
    24181 cattgagcca ggtttttgct tttttacagc tagtacaact tgagactgta taaattttaa
    24241 tcatgtacct acccctttcg ctacatgtta ctatcagttt agtctattat accataaaaa
    24301 acatccgact tgcgacctat ttttaat
//
LOCUS       DAVBHS010000012        22725 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVBHS010000012 DAVBHS010000000
VERSION     DAVBHS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22725
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.012"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..453)
                     /locus_tag="VQ651_000351"
     CDS             complement(<1..453)
                     /locus_tag="VQ651_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET2753398.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVF"
     gene            complement(575..2533)
                     /gene="ftsH"
                     /locus_tag="VQ651_000352"
     CDS             complement(575..2533)
                     /gene="ftsH"
                     /locus_tag="VQ651_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET2753399.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEETGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNREIHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            complement(2549..3091)
                     /gene="hpt"
                     /locus_tag="VQ651_000353"
     CDS             complement(2549..3091)
                     /gene="hpt"
                     /locus_tag="VQ651_000353"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET2753400.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKAREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            complement(3095..4372)
                     /gene="tilS"
                     /locus_tag="VQ651_000354"
     CDS             complement(3095..4372)
                     /gene="tilS"
                     /locus_tag="VQ651_000354"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET2753401.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            complement(4369..5637)
                     /locus_tag="VQ651_000355"
     CDS             complement(4369..5637)
                     /locus_tag="VQ651_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HET2753402.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            complement(5757..6125)
                     /locus_tag="VQ651_000356"
     CDS             complement(5757..6125)
                     /locus_tag="VQ651_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET2753403.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            complement(6118..6384)
                     /locus_tag="VQ651_000357"
     CDS             complement(6118..6384)
                     /locus_tag="VQ651_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET2753404.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            complement(6442..9951)
                     /gene="mfd"
                     /locus_tag="VQ651_000358"
     CDS             complement(6442..9951)
                     /gene="mfd"
                     /locus_tag="VQ651_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET2753405.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYAQHAFIHQLKENG
                     YQKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPIQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            complement(9952..10521)
                     /locus_tag="VQ651_000359"
     CDS             complement(9952..10521)
                     /locus_tag="VQ651_000359"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET2753406.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVKYYLQEKNFEKTMQRYNG"
     gene            complement(10592..11707)
                     /gene="ychF"
                     /locus_tag="VQ651_000360"
     CDS             complement(10592..11707)
                     /gene="ychF"
                     /locus_tag="VQ651_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET2753407.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIIEFRFNV"
     gene            complement(11791..11985)
                     /locus_tag="VQ651_000361"
     CDS             complement(11791..11985)
                     /locus_tag="VQ651_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HET2753408.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(12050..13186)
                     /gene="dnaN"
                     /locus_tag="VQ651_000362"
     CDS             complement(12050..13186)
                     /gene="dnaN"
                     /locus_tag="VQ651_000362"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET2753409.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            complement(13345..14706)
                     /gene="dnaA"
                     /locus_tag="VQ651_000363"
     CDS             complement(13345..14706)
                     /gene="dnaA"
                     /locus_tag="VQ651_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET2753410.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(14919..15677)
                     /locus_tag="VQ651_000364"
     CDS             complement(14919..15677)
                     /locus_tag="VQ651_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET2753411.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(15735..16916)
                     /locus_tag="VQ651_000365"
     CDS             complement(15735..16916)
                     /locus_tag="VQ651_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET2753412.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            17099..17578
                     /gene="rlmH"
                     /locus_tag="VQ651_000366"
     CDS             17099..17578
                     /gene="rlmH"
                     /locus_tag="VQ651_000366"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET2753413.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            17622..17695
                     /locus_tag="VQ651_000367"
     tRNA            17622..17695
                     /locus_tag="VQ651_000367"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17656..17658,aa:Arg,seq:tct)
     gene            17860..17985
                     /gene="comC"
                     /locus_tag="VQ651_000368"
     CDS             17860..17985
                     /gene="comC"
                     /locus_tag="VQ651_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HET2753414.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            18006..19331
                     /locus_tag="VQ651_000369"
     CDS             18006..19331
                     /locus_tag="VQ651_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2753415.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            19328..20080
                     /locus_tag="VQ651_000370"
     CDS             19328..20080
                     /locus_tag="VQ651_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2753416.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            20132..20203
                     /locus_tag="VQ651_000371"
     tRNA            20132..20203
                     /locus_tag="VQ651_000371"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20165..20167,aa:Glu,seq:ttc)
     gene            20209..20282
                     /locus_tag="VQ651_000372"
     tRNA            20209..20282
                     /locus_tag="VQ651_000372"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20243..20245,aa:Asn,seq:gtt)
     gene            complement(20323..20865)
                     /locus_tag="VQ651_000373"
     CDS             complement(20323..20865)
                     /locus_tag="VQ651_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET2753417.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            21044..21811
                     /locus_tag="VQ651_000374"
     CDS             21044..21811
                     /locus_tag="VQ651_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HET2753418.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            21896..22576
                     /locus_tag="VQ651_000375"
     CDS             21896..22576
                     /locus_tag="VQ651_000375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HET2753419.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFVAAISTNMIFAKLPSGRH
                     PESRWAWLKSRAEINGIIAVLAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMV
                     TTLTTWNSRIGAFFLLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQT
                     ISINKSFS"
BASE COUNT         7007 a         4207 c         3700 g         7811 t
ORIGIN      
        1 aaacacaatg cgtaagtctc ttaatactct ttgacgccct cgaaatcgtt cattgcttaa
       61 gacgcgttct agttcttctt gtttctcttt actttgtttg tttctataat ctcttagtgt
      121 ttcatgaaag agataataat catctagcca gagacccccc tcacttatac gatgactgat
      181 ctctcccact tcttcatagg gttctttatc gtatctacgc ttctgacttt cctgcttacg
      241 gatatagtct aaaattcgat ttcgaaactt ggttttgaaa tatttcctta aacgtggaat
      301 atcgtctacc agtccttctt ctctactaat caattcatgt aagcagagca tgccttcttg
      361 gtcccaatcc gacaattccc ataaatgaag gtaatattca tttctacact tataaacagt
      421 cccttggact tcttcataca attctttaat cataattttc ccctttctag atacagtcta
      481 acagattaga aaacacattt gttccatttt ctccattctg ttccctttag ctcctaaact
      541 gcacaaaaaa gagaggctcc agcctctctc agggttattt ttcgtcattc atttttgact
      601 ttacttcatc ataggatagt gcatgagatt cctcttctac tgcttcaggc atctttcctg
      661 tttcgtaaag agctttaatt tgtgtactat ccaatgtttc gtatttcaat aatgcttctg
      721 caattaactt gtgaatttca cgatttgact gaataatttc agcagcttta tttcgtgcct
      781 catttaataa tgaacgaacc tcttcatcaa tttcataagc tgtttgttct gaaattgatt
      841 tttgaggact ctgtgcacca aacatagcat ggtttccttc atattgtact gggccaagtt
      901 tttcactcat accgtactct gtaaccattg cacgcgccat ttgtgtcgct tgttcaaagt
      961 cgtttgaagc tcctgtagtt tggacattaa agataatttc ttcagctaca cgtccaccca
     1021 ttaagccagc caattgctct ttcatatctt ctttagatag aagcatttga tcctctttag
     1081 gaagtgcaat catgtatccg cctgcacggc cgcgtggtac aattgtaacc ttatggacaa
     1141 cgcgagcatt cgacaagact agaccaacaa tggtatgtcc tgcctcatgg taagcaacca
     1201 attctcgttc tttttgtgaa actgtcttat ctttcttaga aggtccagca ataactctat
     1261 cttctgcttc atcaatatct gaagcatcaa ttatcgattt attgcgacga gcagcaacta
     1321 aagctgcttc attcaagaca ttctctaaat cagcaccaac aaagcctgga gtttgttgag
     1381 ccactaattt caaatcaaca tcttctgcta aaggcttgtt cttagcgtga actttcaaga
     1441 ttgcttcacg acctttaaca tcaggacgac caaccaatac ttttctatca aaacgtcctg
     1501 gacgcaaaag ggcaggatca agtacatctg aacggtttgt cgcagcgatg acgataatcc
     1561 cttcatttcc ctcaaaacca tccatctcaa tcaaaagttg gttcaaggtt tgttcacgtt
     1621 cgtcattacc tccgccgaga ccgactccac gttgacgtcc aacagcatca atttcatcga
     1681 taaagatgat agctggtgct gcttttttgg catcctcaaa aagagagcga acacgactag
     1741 ctccaactcc gacaaacatt tctacaaagt cagaacctga gatactaaag aatggaacac
     1801 ctgcttctcc agcgactgcc ttagcaagca aagttttacc tgtccccgga ggtccctcca
     1861 aaagaacacc tgctggaata cgggctccaa gttttgtgaa tcgttttgga tcttttaaga
     1921 actcaacaac ttcaactagt tcttgttttt cttcctcagc tccagcaaca tctgaaaatc
     1981 ttactttaat atcttcttta tttgctgctt tagccttact acgtccaaaa ctcattggat
     2041 tacggccatt gcctcctccc atatttccca tcatagagaa taggaagaag aatagaattc
     2101 caaatggcac aatggatacg agtagattaa tccatatacc acttgaactt tcatgcttaa
     2161 cagttacttc tgctttatgg tcagtagcaa gtttttgcaa ttctgatacg gtagtatctg
     2221 caggaagaat agtgctggta aatttctcta ccttagtaac agatggcgtg aaaaactgaa
     2281 tacctgtttc ttctttactt gttttaggat ttttatagac accagaaact tcgataacac
     2341 taccatttgg ttggtaagtt aattctttta cattaccatc ggtaatttct tgtaccaact
     2401 cagtatagtt gatttgctga cttcctcctg agttattccc agaatagaaa tactggaatc
     2461 ctgtcacaag gaaaaagata aataataacc atagaaaagg atttttaatt aaaccattat
     2521 tttgtttttt cattaaagat tattctttct aatttgaata cacttcctct ttcaatactc
     2581 caatataagg aagattacga taattttctt tgtagtctaa accataacct actacaaact
     2641 catttgggat agtaaagcag gtatagtctg cctcaatttc tacaacacgt ccttctggtt
     2701 tatccaacaa ggttgcaatt ttaacagaag ctgcttctct tgctttaaac atatctcgca
     2761 aattcttcaa agtttgacct gtatcaatga tatcttctac aaatagaaca tgtcttcctt
     2821 tgatatcttg agtcacatct tgtttaatat tgataacacc actacttgct gttccaccat
     2881 ggtagctaga aaccatcatg aagtccattt caatatgtgt atcaatatgt ttgaccaatt
     2941 cagccataaa aggaatagat ccttttaaaa tcccaactaa gattggattt tttcctgcat
     3001 agtctttagt taattgagca cctagttttt tagctgcttc tgtaatttca tcgtgtgaaa
     3061 cgaggacttt tttaatatcg ttttctaaca ttttttacct atctattttt tctatataaa
     3121 gtacagtgtt cattatatca tttttcgttt ttttactcaa attattggtc gcaattccca
     3181 aaattgagac aatttcacca aattgctcaa taataagagc agagtttctc ttttccatag
     3241 ggattttcaa atcaataaat aaacgtctga gtttttttct atgcccattt tgaatcaaaa
     3301 catctcctgt ttttcgatga cgaatgtgta tggatgtttc acgtgaaaca ggtatttgtt
     3361 gaattgattc accttctaat ggaagtccaa aggaaaataa atatccttga taagctacct
     3421 gattttgata gtgtaacaca agttcatctt cctcttcatc agcctgcgga ctgattttac
     3481 aaatctgaaa ctgttggtac tcttttatca attcatagcc atttttaatc ggatgacgat
     3541 actggctttt agattttaaa atctgctgaa cttcagcaaa ctgagctttt gtaagattca
     3601 aatctggaaa acgattcaga taagtttgaa gtaaaactct ttgtgtagac tcagagtaag
     3661 aaaataactg ctgtaaatct tccacattaa tattgttaga taattcagct attgccaaat
     3721 cataatctaa aatttcattg ccaatgccta agattgcatc cctaaatcga ggattttctt
     3781 tttccaattc tggtaagtaa gagtttcgaa tacgatttcg aaaataatga ttctcctgat
     3841 ttgatgtatc ttcaaagtga aaaattgatg gaaagtcttt tttctgaaaa tgcaagaagg
     3901 gacgaatgat ttctatctct ccgactactt gcttctcctt aattcctgat agatagcgca
     3961 agcgagttcc tcgaatcaag cgcatcaaaa tcgtttccac ctgatcatca gcatggtggg
     4021 cagtgactaa agctgtcgca cctgtctttt tcatgacctc ttgaaaaaaa tcataacgaa
     4081 aatttcgtgc acgcgcttct gaaaattctc ctgaaaaatt gctgatataa ataggaagct
     4141 ctgcttcagc agccaacttc cttaattcct tttcttccca atctgattca attctctgct
     4201 tatgattcac atgagctaga atcaattcaa tctctaactc tttttgataa gtagacaata
     4261 ccttaaatag aaacatggaa tctaatccac cagaaagagc tagaaccgcc ttagcatgct
     4321 ttttgaaata tcccttcttg agaaaatgat ttaaaaaatc tggttccctc attttagaac
     4381 ctcataaaca tccttggcta tcttagaaat cgtatcataa tcagaattct tagtgaaaat
     4441 agaaagaata aatggagaat ctgcatagac aacacccgta tcatgcttaa attcatccgc
     4501 atctccaatt ttatgagcta ctttaacaga aacacctttg gcaattcgct gactatcaaa
     4561 atctgtttta gtcaaagact ctaacacaaa tccattttga ttataaatag cttccataaa
     4621 cttcccggcc atcttagaag aaatcaattt ttcttttgga tcccaatcat ctcccataat
     4681 ggcagacatc ttggatttga atgtggcatc agattggttt gaaatgtaat atcccaatag
     4741 attatgagct acattatcag attcttttga tactttcgta attaaatcct ttaaagaata
     4801 ttctttatta tcttcttttt taggaagact accacttccc tctggtttat aagaacctgg
     4861 aaaatcattg actgcagata cgtattttac agtcgtatct aactgataaa gaccctcatt
     4921 tattttttct tgcgtataat agagataaga gagtttcaaa acgctggctg catacatctt
     4981 ttcatcttga ttgataccag cttcttttcc agtagtcagt tgcttaacat aaatagagaa
     5041 agaatctttc tgatattttt cagataacat ttcttgaact ttactcatcc gattatcttc
     5101 ttcagaagtt gattctttag ctacccatcc agcctgatca atatgtagaa attctcttcc
     5161 ttctacaaac atggtcttgt cgattgatac ttgcgaataa gctgataagg atgatttcac
     5221 ttcttttaaa tcataaggac tattgtacag tttaaagtca gattctaacc atgctttttt
     5281 tattgttgga gttacctctg attgatcata taaaaatcgt ttgtccgcag ctataaattg
     5341 atgatttgat agcttaaata ctggaattcc ttgtttattt aagcgccact cggttatttg
     5401 aaaacttgtt ttgggagtca atttcccaga ttccactact aaatcttcat tcgcataaac
     5461 aggaacctct ccataaacca tgggagaact taatttttct ctaaaataaa taccaaagtc
     5521 agattgtgaa aggtaataaa tttctttcga agtatagacg acttcttttt ctgtgctaac
     5581 gacttttgaa atggtcaaaa aacttggtag caacaaaata attaagaatt tacgcattct
     5641 tcctttcctt ttcttcttgt aaacgcaata attgattttt atcagccaat tcttccagct
     5701 tttcatctga caaactcaaa aattgctcta ttacgtctaa taaattttcc attttatcac
     5761 ctttgaagca agtcaggaat cgtataaact ttttccctcg acttagaata atagtacttc
     5821 gctcgtgtat atttggcagc ataatcttca tctttcaact tggtagcaaa tgctgtctcc
     5881 ttatcctttt catcactcaa agtttgatac tgagtttgca agtctgctaa ttgctgacgt
     5941 ctttggagta attgctgata actctgcgct aaattaaaag ttggcaagat aaataatagc
     6001 ataatcaaaa tcaatacccc tcccataaaa cgattccgtt tttgtcgttc tttcatcagg
     6061 tagcgacgac gttggtattc attttgaata aaagaattat tcaattgtac aatattttta
     6121 gacattttct tctacccgtg tttcactgat aatttcatac attcctgctg catcttcttt
     6181 ttttgtacta tctttcatct ctagtacttt tacaagcagc aacttattgc caaagcgaat
     6241 ttcaacttgg tcattaactt tcaagtccgt tgaacttttg gccaagattc cattaacctt
     6301 gattctacct ttatctgcta cttcctttgc gactgtacga cgcttgataa ttcgcgatac
     6361 ttttaaatat ttatctaatc tcatccttat tacctcaatt tattattgta ccatttttat
     6421 ctattttata gaagaaaaat atcaaatgga attttcttcc ttaaactctt ttatctctaa
     6481 taaactttct ccaaaaatca gcagaccttc taaaatttca taatctttct tattttggac
     6541 atcaaataca agctccatta atcccttatt ctcagcgatg cctgctttta agttcgttac
     6601 ggataaagct ttaaaataat cttgagctaa aaacaatcgt tgagtgactt tttcaaattg
     6661 aattgtaatt ttattatctt ttctttccac acgttgaaca aagaccttgt ccaagtatga
     6721 tttaaccaaa ccaatctcta aaagataggc tactacatct gggtattctc caaaacggtc
     6781 tatcaactcc tcttgtaact cttcataatt gacacggttg tcaatttgac gaattttctt
     6841 gtaaatttca atcttatgtc gttgatcaga aatataagta tcaggaagat aggcatcaat
     6901 ttgcaaaatc aactcagcat tccctttggt tcttgtgtta gcgttagcat taccgtttcg
     6961 tttagcaata gcttcctcta ataactgcga atacaattca aaaccaacag aatcaatgaa
     7021 accagactgg gattttccta aaagatttcc tgctccacga atcgaaagat ctcgcattgc
     7081 aatcttaaag ccagagccca attctgtaaa tcctttaatc gcttctaatc tcttttcaga
     7141 gacttcactg attgattttt ctggacgata catgagataa gcataagcaa tacgattact
     7201 acgaccgact cttcctctta actgatataa ggttgacaag cccatatggt ccgcattttc
     7261 aataaataaa gtattagcat ttggaatgtc cacccctgtc tcaataatag tagtcgtcac
     7321 caagatatcg tattgtccct caataaagtc taatagagta ttttccaact ggacttcact
     7381 cattcgacca tgaacatatc caatcgaagc ctccggaatc aactcctgta attctgaaac
     7441 cttctgaaca attgtgtcaa ctttgttgta aagataataa acttgacctc cacgctccat
     7501 ttcacgcaag acagcatcac gaatgacact atcattcttt tccaaaacat aggtctgaac
     7561 aggatagcga ttagtcggcg gagtttcaat aacagataaa tctctgattc ccagcataga
     7621 catatggagg gtacgaggga ttggcgtagc ggtcaaggtt aggacatcca cttgtttctt
     7681 cagttctttc aaagtttcct tatgcttgac accaaatcgc tgttcctcat caataatcat
     7741 caagcccaaa tcagcaaaca caacatcttt tgacaaaaca cgatgtgttc caatcaaaat
     7801 atcgacttga ccgtttttca atttttcaag tgttgcagtc tgctcttttt tacttctaaa
     7861 gcgactcaac acatcaatat taactgcaaa attttggaat cgttccttaa aattcgtata
     7921 gtgctgttgc gctaaaaccg tcgtcggaac tagaatgaca acctgtttgt gatcattgac
     7981 tgctttaaag gctgcacgca tagcaacttc agtctttcca aaaccaacat ccccaactaa
     8041 aagtcgatcc attggctgag aagcctgcat atccctcttg atttcctcaa tactacgaag
     8101 ttgatcatcc gtttcaacat aagggaaagc atcatcaaag gcatcttgat catcatcatc
     8161 agctgagaaa gcaaaaccct tcaactgact acgttcagag tagagtttga ttaaatcatc
     8221 agctatatcc tctacctggt tcttaacctt ttgcttggcc tttttaaaat gaccgtcatt
     8281 taatttattg agttttggag ctttaccatc acttgaaata tatttggaca gtagatgaat
     8341 ctgttccacg gggatagaaa tttgatcacc attttggtat tggacactga cataatcgcg
     8401 atgaattccc ttgatttcaa tggtttcaat tcctagatat tgaccaatcc catggatatg
     8461 atggacaaca tagtcccctt tttcaagttc attgtaatct tttaatctct ctgcatttga
     8521 aacatgttgt cttcgaaaac gacgctttaa tttcttttga aaaatctcat gttcagttat
     8581 caataaaatc ttttcatcta caaaatgaaa accatgtctg agattaccct ctattaaatt
     8641 tacagattct ttacagatac ttgtcttatc tctagaatcc aatttaatct gatattcctc
     8701 taacatatcc tccaatgttt tacttcccat tgaattgcta gactgcagaa taatggtgta
     8761 atccattttt ttatatcgtt caatttcttc ttttagaaaa gaaaactgat tgaaaaattc
     8821 ctgtatagga tattgattga attgataaat tttgtcaaat ttgagatttc ctaaaccctt
     8881 ttgaagatta gagaaaaagg tcactggact ttgtttttta tagatttgtt caatatcaga
     8941 aaaatactgc atatcagaaa atgctttact attctgtaat tcttctgtaa agtactgcgc
     9001 taattctctt tcaaagactt catactgatt catcaatttt tgataatcat caaagaatat
     9061 tggagtatct ttttcaatat aatcaaagac agtccatgtc ttatcatagc acaaagataa
     9121 aaacttccga gaatctgcat gactttgttt ttggtgaaaa cttgaaagaa tttcttctag
     9181 gtatgatttc aaaataggtg ataaagtttt tgaaatttgt ttttctaaag ctgactgtcc
     9241 tcgttgataa tccttttctc tcaaaagcat atcactagct ggaaagatag tgagttctgt
     9301 cttattttct ttcgataatt gtgtttctac ttcaaatgac ctgataccat caatttcatc
     9361 accaaaaaac tcaattcgac aaggttctaa ctgggatatt tcaaaaatat ctaaaatatc
     9421 tcctcgaaga ctaaattcgc cctgagtttg tacttgagta actttttgat agccattttc
     9481 ctttaactga tggataaacg cgtgttgagc atattcttca ccaactgaga tttttacaat
     9541 actatctttg aatgcattgg gagacggtaa aatcaatcga cttgctgcga tattacaaac
     9601 taaaatccct ttcttagatg aatcagtcaa aaaacgcaag gcttcaaccc gtgaaataat
     9661 tttttcctgt gaagacatca aaaactccac cataggagca tcatctacca aaaatggata
     9721 gacgagttcc tcacccaaga tagaaataag atcactaaca agtccttctg cttctccata
     9781 agttgacgtc aataacacaa tcctatcttc tttttctaaa ctgcttgcaa ttgcaagagc
     9841 cttagtagat gttgataaac caagtattag ttgtcttttc ttatctgtta aattttgatg
     9901 ccatttttta atctgatcat tttctgagaa taaatctaat aaggtcacca tttatccgtt
     9961 atacctctgc attgttttct caaaattttt ctcttgtaaa tagtatttta cagaatcgtc
    10021 aactttgtca acagactgta aaataccgat ataatcatcc ctgtcaaact tactcaaaac
    10081 atgatgaaca actgacatac catttttagg tcttccaatt ccaatcttaa cacggttaaa
    10141 gacctgagtt cctatatgtt gaataataga cttgatacca ttatgaccac ctgctgagcc
    10201 ttttgctctt aaacgaattt tcccaacttc catgtcaaga tcatcgtaaa tgataagtaa
    10261 atcgtcaata tccaaaccat agtaagttaa taaagcatga actgcttttc cactttcatt
    10321 cataaaggtc gttggtttaa ccagataaat tttttctcca tttaggaaaa aggatgctag
    10381 gtcagcttga aatatcttat cgtgtgtaaa agtgacattc tgtttcttcg ctagttgatc
    10441 aatcaacata aaaccaacat tgtgttttgt ttcaaaatat ttatcccctg gatttcccaa
    10501 acctacaagt aatttggtca tttatttttc ctttcaaaag ccaaaagggt tggaattttt
    10561 ttccaaccta attgacacca tttattaatt tttagacatt aaagcggaat tctatgatgt
    10621 cgccatcttg aacgatatat tcttttcctt cttcacgcaa gcgtccagct tcttttacgg
    10681 ccttttcaga tccgtatttc actagatctt catatgacat ggttactgca cgaataaagc
    10741 ctttttcaaa gtctgagtgg ataataccag ctgcttgagg agccttcata ccacgtttga
    10801 aagtccaagc gcgaacttct ttttcaccag ctgtgaagta agttcccaat ccaagcaagt
    10861 ggtaagctgc acgcgtcaac ttatctacac ctgattctgt caaaccaatg gcttcaagaa
    10921 actctttttt atcttcatca tccaattcag aaatttcttc ctcagcacgc gcagaaataa
    10981 cgactacttc agcattttct gtcgctgcaa attcacgaat ttgtttgaca tagtcgatag
    11041 agtcaggttc tgaaaccaca tcctcgtcca cattagctac ataaagaact ggtttagtcg
    11101 tcaaaaggaa aagacctttg acaacctttt gttcctcatc tgtaaattca atggtacgag
    11161 ctgatttccc gtcttctagg actggtttaa tcttttgaag aacattgaat tctgctactg
    11221 attctttatc tttttgcgta cgtgccatct tttctacacg cgcatatcgt ttgttcactg
    11281 attctaagtc agcaagaatt aattccagat taattgtatc aatatctgca agtggatcta
    11341 caaaggcgtc ttcacgtcct tgctcgcgca tcacattttc atcatcaaaa gcacgaacta
    11401 cgtgaacaat cgcatctact tcacgaatat tggccaagaa tttattccct agcccttctc
    11461 cttttgaagc tccttttaca atccctgcaa tatccgtaaa ttcaaatgtt gtgggaactg
    11521 tctttttagg agttatcatt tcagttagtt tttgtaggcg ttcatctgga acttccacca
    11581 ttccaacatt tggatcaatc gtcgcaaatg ggtagtttgc tgcctctgct cctgcttttg
    11641 taattgcatt aaatagtgtt gatttaccaa cgtttggcaa accaacgata cctgctgtca
    11701 aagccatatt tctcattctc cgttttcatt tcaatcccta atattataac acaaaaaggg
    11761 ataaagtgct aaccctctaa ctaagggttc tcagtcaata attttattca tttttcgctc
    11821 aaaatcatat cgccccatca tgacaacatg ctcacaatta ctgcatttta ttttgatatc
    11881 tgctcctaca cgtgtaattt cccaacgatt agccttttta ccagtcgact tgattgtaca
    11941 agcgtgtgat tttttcatct caacaaaatt tccaacttga tacatactac tctccttttc
    12001 tttttcgatt atatcataaa agaggcgagc caggctcaac ctctttcact taatttgtac
    12061 gaactggtgt aatgagctgc atgaagtctt cgtcagtatc tgctggcaca agagtaaatg
    12121 gacgaacagc tgagataaag ctaatggtca ccttttcgct atttaaagct ttaagagaat
    12181 caatcaagta agttgggttg aaactaatgg tcaaatcttc accagtaacc tgatcagtat
    12241 cgatttcttc gtttacttta ccaacttctg gagagtgaac atgggcgcta acaaccccat
    12301 ccttaatttc aagtttcaca gtaccatttt gagtcgcact tgataaaaga cgggcacgct
    12361 ccattgactg gcgtaagttt accacattaa aagtaatagt agtgttaaag tctgttggaa
    12421 tcaagcgatc tgtatcagga tagtttcctt ctaggagacg agtatagaag ctaatatttt
    12481 cgcttctaaa gaggatttgg ttattggcaa agaaaatctc tacagtttcg atatcatctg
    12541 taaataccgc tgaaaattcg cgtagagaac ggctaggaat tacgacatca aaatcatcac
    12601 tatttttttc aagagtcaat tttttctggc ttaggcgatg agagtctgtt gcaactgttt
    12661 ttaactcttt gtgttgactc aatacgaagt ggacacctgt taaaatcgga cgactctctt
    12721 gtgtacttgc agcaaaggct gtttcattaa taattttctt gagtaatttt gtttcaagta
    12781 ttaaaggagt gcttgctgaa atttcttgga ttcgtggata ttgttcgcta tcttttcctt
    12841 ttagggtaat ttctgatttg ccactggtta aaacaatttg attttgttca atttctttaa
    12901 aatcaagagt tacatcaggt aaactagata ctacattgat aaagaaagaa gcttcaagaa
    12961 ggatcgaacc taaagaagta attaacaaac cagcatcttc atttttttga gaaataaaat
    13021 tttcaattga aatttgacca tttgaaccaa ttaaagtaat accttcattg gtcacgtcaa
    13081 tttttactgt tgataaaata ggaatggcat ttttagaact aatagctctc ttagtagtat
    13141 ttaatgcttg tagaaataaa tttttattaa ttgaaaaatg aatcatggat tctcctttat
    13201 ttatttttag tattagaaag ataatagtaa taatagagtc tgtgaaatct gtggaaaaca
    13261 gcctttaaac aagtcatatc aagtttttta gcttgtttaa aaaatgtgga taaaaaagat
    13321 aaaaaactaa aagttatcca caaattattt gattttcttt ttgattgatt caatttctaa
    13381 acgtaaatta tcgtcttgat caatcaaaga ttttattttg gcatgggcat gaatgactgt
    13441 ggtatgatct tttcccccaa attccttccc aatttttgga agactattat ctgttagttc
    13501 tctagataaa tacatggcta cttgacgggc caaaacaata ttttgaaggc gtctacttcc
    13561 cttcatttct ttgatactaa caccataaaa gttaccaact tcagtttgga ttttatcaat
    13621 tgggatgacg agcatttggc taacatcttg tttgcgggct ctaatggctt ctgcagcaat
    13681 atcaatagtg atatccttga ttttttttac tctggcaatt aaagtgatgt cgttgatggc
    13741 tccctcaaga tctcgaacat ttgaatcaaa ttgcccagct aggtattcta gagtatcact
    13801 ttggaaattg tagcctaaat gttccgtttt actttgtaaa atggcaatac gtgtttcaaa
    13861 gtcagggggt gtgatagttt gtgtcaatcc ccaactaaaa cgcgtgacaa gcctctcctc
    13921 gagcccttct agatgttttg gactacgatc actcgttagg acaatctgtt tttgcttgtc
    13981 atgaagggcg ttaaaggtat tgaaaaattc ttcctgagtt gcgacttttt ttccgctgag
    14041 tgactggata tcatcgatta acaaaagatc aagactacga taggtctttt taaacttttc
    14101 catttcccca agtcttaggt gatcaagaaa gtcattaata aagctttcgg cagggatata
    14161 tttaacacgc gcattaggaa tattttttag aatttcattt ccaatagcgt ttaataagtg
    14221 agtcttacca aggcctggtc ctccatagat aaaaagaggg ttataggtca gagccaaatc
    14281 ttcagagaca gctaaagcgg ctgacacagc ccaaacattt ccatcccctt gaataaagtt
    14341 atcaaaggta tacttttctt ttaatcccgt atctgaataa ggaatagata ctaactttgg
    14401 actatagtta taaagagtta aatttgtagc ttcttcaact tgtgagctag tcgtatcttg
    14461 aggtttggtg aaaatatagt ggggagttat ttcagcgtca taaatttcaa aaccagctac
    14521 tacaataata tcttttagtt gtttttccca gaccatttcc atttcagagc gaggtagaaa
    14581 tatagtggca acattttcct ctaccttgat gagttcagct tgaatagcat agaaatcata
    14641 catggatcga gtcagtcttt cttgtgcaaa ttctaatata cgattccaaa attgtttttc
    14701 tttcaatcct ttctcctcct ttactattta aaagtttaat actagagata ttttaccata
    14761 gatagcaaga attttccaca agctgtgaaa aataatccac aatgttacca aactttatcc
    14821 acaggttggg gataaaagaa gaaattattg atttattaag ctttttattg aaaaacatag
    14881 tggataacct tgtgagataa aaaaataaaa gaacagcctt atttcaggct gttgataatt
    14941 ctactatatt cttcttgatt tgaaaaagaa ataatgattt ttccactgtc ttttttagat
    15001 agtttaattt ctacatctaa tccgagtagt tttcttaact gtttttcttc attttgtatg
    15061 aaatgattag ttttttgctg tttcttttgt tttttctctg tcagaagagc ttctaatttc
    15121 cttacagaaa tatcttcttc tataatccgt tgaaagaaat agtcttgttg ttccttattt
    15181 aacccaacta gggaacgcgc atgggcttgt gatagtttgc cattttctac ttctgaaaga
    15241 atctgttctg gcaaggaaag taaacgaatg gagttgctga tatatggacg agacttgccc
    15301 atcttatctg caatttcagc atgggtgaat cctttctcta cgagagattc ataggcgcgt
    15361 gcttcttcta ttgggtttaa attttctctc tgtaaatttt caataatgga ctggaccatc
    15421 atctcttggt ctgaaatctg tttaacaaca gctgggatag accgtagacc agctaaaagt
    15481 gaagcccgat agcgtctctc tcctgcaagg atttcataac caataacagg agattgacga
    15541 acaataatcg gttgaatgac cccattttct ttgatagact gtgctagttc atctagtttt
    15601 tctctatcaa attcttttcg gggttgatag ggattttttt gtatatctgt gatagaaatc
    15661 atttcaaatt tttccatgat tctacactaa cacatctttt ctcttatgta aagctttctt
    15721 tacatagatg tcaattaaga ttctaaatca cctgaactct tgttaagttt gatagaggta
    15781 gtttcttctt tcccgttacg atagtaggtt atcttaatgg tgtctccgat agaatggttg
    15841 taaagagcac tttgtaagtc tgttgatgaa gcaatctctt tgtcatctac ttttgtaatt
    15901 acatcgtatt tttcaaggtg accattggca ggcatattac tttgtaccga acgaacaact
    15961 acaccagatg taacattact tggaatattg agtcttctga tgtcgcttgt actcacatta
    16021 gataaattaa ccatctggat tcccaaagct ggacgcgtca cttttccgtt tttttctaac
    16081 tgttcaataa tattgatagc atcatttgca ggaattgcga aaccaagacc ttctacagat
    16141 gttcctccat ttgtagcaat tttacttgag gtaattccga taacctgccc ttgaatattg
    16201 atcagtgggc cgccagagtt acctgggtta atagcagtat cagtttggat ggcttttgta
    16261 gaaatagctt gtccatcttc cgattttaag gatacatttc tattgagact ggatacgata
    16321 ccttgagtga cagtatttgc atattcagaa cctaacgggc taccgatggc aatagcagtt
    16381 tctcctacag ttaacttact agaatcacca aactcagcta ctgttgtcac tttttctgaa
    16441 gagattttta cgacagcaat atcagagaaa gtgtcagctc cgacaatttc tccaggtact
    16501 ttagtcccat ctgacaatcg aatatctact ttgctggcgc catttataac gtgattgttg
    16561 gtgacgatgt aagcttcttt atcattcttt ttataaataa ctccagatcc ttcactagag
    16621 attcgctgag aatctgtgtc agtatcatca ttgccaaata cgctattttg tctgtttgcc
    16681 gaataagtaa taacagaaac aacagcatct tttactttgt taacagcctg tgttgttgaa
    16741 ttttcgttct tataggcagt ttgtgtaata gtactattgt tgttagagtt gtttacacta
    16801 cttttttgag ttagttgagt tattgaaaaa ctacccaagg ctccactaaa aaagctaatg
    16861 acgataacga ctaataattg aaaccatttt ttgtaaaatg tttttagatg tttcatattt
    16921 gcctccatat gtttgaatta ctgaaagtat aaactgacta gcttaattat aacttaaaca
    16981 caaaagtttt acacaaactg tggataactc ttttgaaact gtgattttct taattgaaat
    17041 ctatttttta ttttgtgaat aagatgtgaa aaaatagaga atatgttaga atagagtcat
    17101 gaaaattaaa gttgtaacag ttgggaaact gaaagaaaag tatttaaaag atggtatcgc
    17161 agagtattca aaacgaattt ctagatttgc taagtttgaa atgattgagt tatcagatga
    17221 aaaaacacca gataaggcca gtgaatcaga aaatcaaaag attttagaaa tagaaggtca
    17281 gagaatttta tcaaaaattg ctgaccgtga tttcgttatt gtgttagcca ttgaagggaa
    17341 aactttcttc tcagaagaat ttagtaagca gttagaagaa acttctataa aaggattttc
    17401 tactcttact tttattattg ggggaagttt aggattgtca tcatctgtaa aaaatagagc
    17461 caatctttct gtcagttttg gtcgcctaac cttaccccat cagttaatga gactagttct
    17521 tgttgaacaa atctatcgcg cttttacgat tcagcaggga ttcccctacc ataaatagaa
    17581 aattgacttt taattgaatt tttggtagaa taattgtatt aggtctcata gctcagctgg
    17641 atagagcatt cgccttctaa gcgaacggtc gcaggttcga atcctgctgg gatcaatata
    17701 atagcaaagc tgggaatttt cccggctttt ttcttaaaaa agtacacttt gggagaaaaa
    17761 aatgacagtt gagagaattt tatctaaaac gaaattccat tttgtataat ggtttttgta
    17821 agttagctta caagaaaaaa cattttagga gattttatta tgaaaaacac agttaaattg
    17881 gaacagtttg tagctttgaa ggaaaaagac ttacaaaaga ttaaaggtgg ggagatgagg
    17941 ttgtcaaaat tcttccgtga ttttatttta caaagaaaaa agtaatgaaa taaggggaaa
    18001 gagtaatgga tttatttgga tttgggacgg ttattgttca ttttttaatt attagtcaca
    18061 gttaccattt tatttgtaaa ggtcaaataa atagaaaaga attattcgtt tttggtgctt
    18121 atacattact aacagaaata gtatttgatt ttcccttata tattctatat ttagatgggt
    18181 tagggattga aagattttta tttcctttgg gattatattc ctattttcga tggatgaaac
    18241 agtatgagag ggatagagga ctattcctaa gtttactact atctctttta tatgagagca
    18301 ctcataactt tctgtccgta actttctcct ctataacagg agataatttt gttttacaat
    18361 atcatttccc attctttttc gttgtgacgg tgttaaccta ttttgttaca ttaaaaatca
    18421 tttactattt ccatttggaa ctagcctatt ttgacgagga ctacctttat cctttcttga
    18481 aaaaagtatt ttttgcttta ctattgctac atattgtatc tttcgtttca gatatggtaa
    18541 gtacgattaa acatttgaat agttttggaa gtattttgtc atctattgtc tttatctctc
    18601 tccttttgac cttctttgca atgaattctc ataaagttca aatggagaaa gagattgctt
    18661 tgaagcagaa gaaatttgaa cagaaacatt tacagaatta cacagatgaa attgttggtc
    18721 tgtataatga aatccgtggt tttcgacatg attatgctgg aatgcttgtc agcatgcaga
    18781 tggcaattga cagtggtaat ttacaggaaa ttgacagaat ttacaatgaa gttttagtca
    18841 aagcaaatca taaattgcgt tcagataagt acacttactt tgatttgaac aacatagaag
    18901 actcagcttt acgaagtttg gttgctcagt caattgtcta tgctcgaaat aatggtgtag
    18961 agtttacact ggaagtaaaa gatacgatta ccaagcttcc aattgaacta ttggatttgg
    19021 ttcgtatcat gagcgtttta ttgaataatg ctgtcgaagg atcggctgat agctataaaa
    19081 agcagatgga agtagcagtt attaagatgg aaactgaaac agttattgtg attcagaatt
    19141 catgtaaaat gacgatgact ccttcaggag atctatttgc cttaggattc tccactaagg
    19201 gaagaaatcg cggagtcgga ttaaataatg tgaaagaact actagataag tacaacaata
    19261 ttattttaga aacagagatg gaaggcagta catttagaca aatcattaga tttaagaggg
    19321 aatttgaatg aaagttttaa ttttagaaga tgttattgaa catcaagtga gactagagag
    19381 aatattggat gaaatttcga aagaatcgaa tattccaata tcatacaaga caacgggaaa
    19441 agtccgtgaa tttgaagaat acattgaaaa tgatgaagta aatcagcttt atttcctaga
    19501 tatcgatatt catggaattg agaaaaaggg atttgaagtg gctcagctca ttcgtcatta
    19561 caatccttac gctattatcg tctttatcac tagtcgatca gagtttgcga ctctaaccta
    19621 taaataccag gtatcagccc tagattttgt tgataaggat atcaatgatg agatgtttaa
    19681 gaagagaatt gagcaaaata tcttctacac gaagagtatg ttacttgaaa atgaagatgt
    19741 tgtagattat ttcgactaca attacaaggg aaatgattta aaaattcctt accatgatat
    19801 tttgtatatt gaaacaacag gggtctctca taaattgcgc attattggta agaattttgc
    19861 aaaagagttt tatggtacca tgacagatat tcaggaaaag gacaaacata ctcagcgatt
    19921 ttattctcct cacaagtcat ttttggtaaa tataggcaat atcagagaaa ttgatcgaaa
    19981 aaacttagaa attgttttct atgaagacca tcgttgtcct atttcaagat taaaaattag
    20041 aaaattaaaa gatattttag agaaaaaatc tcaaaagtga ttgacaatta gcaagaaatt
    20101 gatataatgg ttatatctgc tacagataga aggtccgttg gtcaaggggt taagacaccg
    20161 ccttttcacg gcggtaacac gggttcgaat cccgtacgga ctattttatc cgccatagct
    20221 cagttggtag tagcgcatga ctgttaatca tgatgtcgta ggttcgagtc ctactggcgg
    20281 agtatagata aaagggaaca cagctgtgtt cctctttttg tatcaatttg tatcaccaag
    20341 cattttcata aggaagtctg ttatttcttg agaactttct ttttttccat gtgcaatcca
    20401 agtttggcag acaccaaaaa gtgcatgagt tagatagatg ctactatatt ctaattcggt
    20461 ggtatttaga ttcagttgca taaatcgctt ttgtaaatct gtactaagca tgatatgaag
    20521 tttatttcgt aagaaatttt ggatttcttt agtcccattt tcagaaagaa gggcagccag
    20581 aagtggttct gactctagat attcaaaaac ttctaaaata gcgtctcttt tgtgatgagc
    20641 atgtttttga aaaatatatt caaatgtatg gaatagcttg ctttgatagt gctcaatcat
    20701 atcatactta tccttatagt gagtatagaa gctggaacga ctaattccgg ctttttctac
    20761 taatttgaca gtagaaattt tatcaaatgg ctgttccatc agtaattgta ccatagcatt
    20821 ttcaatagtt cgctttgttt ttaagcgttt gttactttct tgcatatttc ctccttgtaa
    20881 acaaattaga ctatatgttt aaaaatagat tttttatctt gtaatttaga ttttttaatg
    20941 tataatctat tatatcaaaa ttttagacaa tatgtttaaa aaaggagaaa ctaagtttaa
    21001 agaatggaaa gcaatttaaa aaaaaccaac ctttattatt gtcatgatcg ggatttctct
    21061 tattccagat ctgtacaata tcatattttt gtcatcaatg tgggatccat atgggcaatt
    21121 gtctgactta cctgtggcag ttgtaaataa tgataaagag gcttcctata atggtaatac
    21181 tatggcaata ggaaaagaca tggtgtccaa tttaaaagaa aataaaacct tggattttca
    21241 ttttgtagat gaagaggaag gaaagaaggg attggaagat ggcgattact atatggtagt
    21301 gactttacca agtgatttat ctgaaaaaac aactacatta tccaatattc aatcgacagc
    21361 agcttatcaa tcattgacaa gtgagcaaca aactgagata agtgattctg tatctcaaaa
    21421 ttcaactgat agtattcaat cggctcagtc aattgtagct ttagtacaag atttacaggg
    21481 aagtttagaa aacttacaaa atcaatcttc taatctttcg actttaaaaa atcaatctaa
    21541 tcaagtatca cctattactt ctacttcttt gataggattg tcaagtggat taacagagat
    21601 acaaggagat gttactagca aattagttcc tgccagtcag tcgattgcat caggtgtaaa
    21661 cgcatatact acaggtgttg ataaagtttc tcagggcgca agtcaactaa gtgaaaaaaa
    21721 tgccaccttg acaggtagtt tggataaact agtttcaggc tcaaacacct tgacacaaaa
    21781 atcttctaga ttgacagcag gagttggtta attacaatca ggatctgggc aattagcaga
    21841 caaatccagt cagttacttt caggtgcttc tccattagag aatagagcta ataaattggc
    21901 agatggatct gggaaactag cagaaggtgg aacaaagtta acttctggat tggaagattt
    21961 acagacagga cttgcttctt taggacaagg actaggtaat gctagtgatc aactcaaatc
    22021 agtatcaaca gaatctaaaa atgcagagat tttgtcaaat ccactcaatc tttcaaaaac
    22081 agacaatgat caagttcctg taaatggaat cgcaatagct ccttatatga tatcagttgc
    22141 tctttttgtt gcagcaatat caacaaatat gatatttgcg aaattgcctt caggacgtca
    22201 tccagagagc cgttgggctt ggttgaaatc tcgagctgaa ataaatggta ttatagctgt
    22261 tttggcagga attttggtat atggaggagt tcagcttatt ggtttaactg ctaatcatga
    22321 gatgagaata tttattctca tcatcctaac aagtttagta ttcatgtcta tggtgaccac
    22381 tttaacaacg tggaatagcc gtataggagc ttttttctta cttattttgc ttttactaca
    22441 gttagcatca agtgcaggta cttatccact tgctttgaca aatgatttct ttagatctat
    22501 taatccctgg ttaccaatga gctattcagt ttcgggatta cgacaaacaa tctctatcaa
    22561 caagtcattt tcctagctgt catactagct ctatttactg gtttaggtat gctagcctat
    22621 caacataaga aaatggaaga agattaaaaa aatcgaccga ttaatgaact gcaccccaaa
    22681 agttagacag aaaaaatcta acttttgagg tgttttatta tgaaa
//
LOCUS       DAVBHS010000013        22592 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVBHS010000013 DAVBHS010000000
VERSION     DAVBHS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22592
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.013"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..69
                     /locus_tag="VQ651_000376"
     CDS             <1..69
                     /locus_tag="VQ651_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
                     /protein_id="HET2753420.1"
                     /translation="YKIMMHLSDTFNSWDYRQVEYY"
     gene            232..2163
                     /locus_tag="VQ651_000377"
     CDS             232..2163
                     /locus_tag="VQ651_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HET2753421.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELMSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNESDENDDADY"
     gene            2150..3502
                     /locus_tag="VQ651_000378"
     CDS             2150..3502
                     /locus_tag="VQ651_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HET2753422.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKKQKILETINQEIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSDYSAFCKMMENSEENFKAIIDEEVGRK
                     "
     gene            3677..4663
                     /locus_tag="VQ651_000379"
     CDS             3677..4663
                     /locus_tag="VQ651_000379"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HET2753423.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKQMHDQGLIASISVGVKDYEY
                     DFVSQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            4727..4798
                     /locus_tag="VQ651_000380"
     tRNA            4727..4798
                     /locus_tag="VQ651_000380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4760..4762,aa:Arg,seq:ccg)
     gene            4981..5679
                     /locus_tag="VQ651_000381"
     CDS             4981..5679
                     /locus_tag="VQ651_000381"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET2753424.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            5670..9209
                     /gene="smc"
                     /locus_tag="VQ651_000382"
     CDS             5670..9209
                     /gene="smc"
                     /locus_tag="VQ651_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET2753425.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKSEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAITVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDAVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLRSEEAALKTLQDQMATLTERLEVIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQDRLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRYLQSQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKDRFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            9206..10000
                     /locus_tag="VQ651_000383"
     CDS             9206..10000
                     /locus_tag="VQ651_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2753426.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGETWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            10000..10818
                     /locus_tag="VQ651_000384"
     CDS             10000..10818
                     /locus_tag="VQ651_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2753427.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKATLKAASDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGHEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            10822..12042
                     /gene="ftsY"
                     /locus_tag="VQ651_000385"
     CDS             10822..12042
                     /gene="ftsY"
                     /locus_tag="VQ651_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET2753428.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAVLEESQDSEPAVGVSQEEIEELPEEEKLEHEGTIEENNSEVLEAERPQTEETVQ
                     EKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEELLIMSDVGVQVASNLTEELR
                     YEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQDNLTVMLFVGVNGVGKTTS
                     IGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVDVPVVTGPEKADPASVVFDG
                     MERAVAEGIDILMIDTAGRLQNKDNLMTELEKIGRIIKRVVPEAPHETFLALDASTGQ
                     NALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREELNIPVKLIGFGEKIDDIGEF
                     NSENFMKGLLEGLI"
     gene            complement(12088..13575)
                     /locus_tag="VQ651_000386"
     CDS             complement(12088..13575)
                     /locus_tag="VQ651_000386"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET2753429.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPAIFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRFE"
     gene            complement(13724..14464)
                     /locus_tag="VQ651_000387"
     CDS             complement(13724..14464)
                     /locus_tag="VQ651_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2753430.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDNLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(14464..16629)
                     /locus_tag="VQ651_000388"
     CDS             complement(14464..16629)
                     /locus_tag="VQ651_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET2753431.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            16802..17755
                     /locus_tag="VQ651_000389"
     CDS             16802..17755
                     /locus_tag="VQ651_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2753432.1"
                     /translation="MKNKRIFKDFQASKMNLNLYTSPLLAFVFVFIGEFVAYTLYGIS
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENCLSNLLKGFSLGLALFLLTLLSLVVLGQYRLESIHLNPYYLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            17832..19820
                     /gene="uvrB"
                     /locus_tag="VQ651_000390"
     CDS             17832..19820
                     /gene="uvrB"
                     /locus_tag="VQ651_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HET2753433.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            19830..20393
                     /locus_tag="VQ651_000391"
     CDS             19830..20393
                     /locus_tag="VQ651_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2753434.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKTNDGYYQINGNS"
     gene            20359..20847
                     /locus_tag="VQ651_000392"
     CDS             20359..20847
                     /locus_tag="VQ651_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2753435.1"
                     /translation="MTVIIKSMETPEEIEGKPLVHWQTWREAYDGLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAMDGMKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LIKAALIALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDEQEKILELGKPIKEKRMVFY
                     SK"
     gene            20920..21387
                     /locus_tag="VQ651_000393"
     CDS             20920..21387
                     /locus_tag="VQ651_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2753436.1"
                     /translation="MSRAQLTILTNICLIEDLEAQRVVMQYRAPETNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVVCYKATEFSGTLRS
                     SDEGEVSWVQKDQIPNLDLAYDMLPLMEMMEASDKSEFFYPRRTEDDWEKKIF"
     gene            complement(21384..21899)
                     /locus_tag="VQ651_000394"
     CDS             complement(21384..21899)
                     /locus_tag="VQ651_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HET2753437.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFKPLSELTDGVHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(21911..22438)
                     /locus_tag="VQ651_000395"
     CDS             complement(21911..22438)
                     /locus_tag="VQ651_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2753438.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGSLYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
BASE COUNT         7038 a         3994 c         4988 g         6572 t
ORIGIN      
        1 tataaaatca tgatgcacct gtcagatact tttaactcat gggactaccg tcaggttgag
       61 tattattagg atagagaaga agtctagtgt taaggacatt acagctcata atactaggct
      121 tttttataga aattcttttc tttttctata ggatatggta ttctatgtag aaaatatatg
      181 ttaataagta atgccaatat ttaaacatca ttagtaaaag gagttagatt gatgaataaa
      241 agaaaagtta gtttagaaga tttttataaa tggtatagtc taaataaaga agagttatta
      301 aataaggcaa ctgttggtga aaagtttaat gataaattaa aagaagagtt tctccaggaa
      361 tggcctttgg ataggatttt aacaatgtca atcgatgaat atgtaatagg aaagggacag
      421 caaaataagt ctttatgcta cgctcttgag aagggaaaat acaaaaatct atttcttgga
      481 atttctggtg gctcagcttc aaaatttggt atttattgga ataaaaaaac aaacaaatat
      541 aaagatcaag ctaataatga gatttcagag ttggatcagc gattttcaaa attaaaatca
      601 gatttgtatg aaattatcaa agaaggtatt cgttttaact ttgaaaatcc tatttttgat
      661 atgaaaagat caacaaatga atttattggt cgttctgcta tggtgacaaa attactttgt
      721 atctatactg agggagatcc tttctttggt gtaaatatta atagtcagaa agaattttgg
      781 aaccactttg tttctcagac aaatcaaggt ggaccttatc tgcaaaatca taaaataatt
      841 gaactgatgt ccaaaactta tcctgagttg gagccatcga aattaggaac tatgcttttt
      901 gagtattcta agctttttat ggaaaataag gaagacaata gtacaatgga ttcatcaaac
      961 aattttcgtc atcaattaac tcaatctcta ttaaagtctc caaaccttat ccttcgtggt
     1021 gctcctggca caggaaagac ttatcttgct aaagaaattg ccaaagaatt aacggatggc
     1081 aacgaagatc aaatcggatt tgtacaattt cacccatcat atgattatac ggattttgta
     1141 gaaggtttaa gaccagtatc aaatggggat ggagctattg agtttaggct acaggacggt
     1201 atttttaaag atttttgtca gaaagcaaaa gaaacccaat tgattggagg acaagataat
     1261 tttgatgagg cttgggattc ttacttagaa tatataaatg ttgctgaaga aaaagaatat
     1321 ataacaaaaa catcttactt atctgttaat agtagacaaa atttgtcagt aaattatgat
     1381 agtggtgttc caggatggtc actacctagc aaatatgttt acgagttgta taaagataaa
     1441 aattataata agcaagaata ctacaaaagt ggtggaaaaa ctgtcctaga aacattgaga
     1501 aagagatttg gtttgaaaga ctatgtttcc ccaacagaaa ttgatactga taagaaattc
     1561 gttttcatca tcgatgaaat caatcgtggg gagatttcta agatttttgg cgaactcttt
     1621 ttctctatcg accctggcta tcgtggtgaa aaaggaagtg tttctaccca atatgcaaat
     1681 ctacacgaaa ctgatgaaaa gttctatatc cccgaaaatg tttacatcat cggaactatg
     1741 aatgatattg atcgttcagt ggataccttt gattttgcta tgcgtcgtcg ttttcgtttt
     1801 gttgaagtta ctgccgaggg tcaagttggc atgttggata aagagctgaa tatccatgca
     1861 gaagaagcaa aaattcgtct aagaaacttg aacgctgcta tcgaaaatgt tcaggaatta
     1921 aacagtcatt atcatattgg accaagttat tttcttaagt tgaaggatgt agattttgac
     1981 tatgaattac tctggtccga ttatattaag cctctcctag aagactactt gcgaggttct
     2041 tatgatgagg ttgaaacttt ggaaactttg aaaaaagcat ttgaactgac aaataatgag
     2101 caaaaagatc aggcagtagc tgatgacaat gaaagcgatg aaaacgatga tgcggattac
     2161 tgataatcaa cacaagatta ttaaagaaaa atttgttgaa gaatatccta aactaagcaa
     2221 tcttctttta gacagaacct tggaaagtct atcccaagat gaacgtattt tcatttttcc
     2281 aaatgatttg actcatactc cagatttgga taaaaaacaa aagattttgg aaacgatcaa
     2341 tcaggaaatc aagacaggaa atgtgattgg ttttcttgga tatggtcagg aaagattaac
     2401 gatttcctca cgattttctg atgagagtaa tgaccatttt ttgcattatc tcttaaacaa
     2461 ggttcttcat atcaatctca ctagtttaga tgttgctttg tctcgtgaag agaggcttta
     2521 tcaacttttg atgtatctct tccccaagta tctacaagct gctattcgaa aaggtcttta
     2581 taaggaatat catcgatttt ctcataacga cagtcatgtt aagggagtga ttgatgtaag
     2641 aaaccatctc aagaaaaatc ttcctttcac gggaaatatt gcctacacaa cgagagagtt
     2701 cacctatgat aatcccctca tgcagttggt ccgtcacact attgaataca ttaagaatca
     2761 gaaaagcatt ggtcaagggg tactagataa tctctcaact agtcgtgaaa acgtatctga
     2821 aatcgtgcgt gtaacgccct cttataaact agctgatcgt gctaagatta ttcggggaaa
     2881 tcaatctaaa cctatacgtc atgcatactt tcacgagtac agaaacttac aagaactttg
     2941 tctgatgatc ctaaaccaag aaaagcacgg tttagggtat caagatcaaa aaatctatgg
     3001 tattctcttt gatgttgcct ggctttggga agagtatgtt tacaccttgt tgccaaaagg
     3061 ttttgtacat cccagaaata aggataagac ggatggaatt tcagtatttt ctgttgggaa
     3121 acgaaaagta tatccagatt tttatgacag agaacgaaag attgttctag atgcaaaata
     3181 taaaaaactg gaattgactg aaaaaggaat caatcgtgag gacttattcc agctgatttc
     3241 ctattcttat attttaaaag ctgagaaggc tggacttgtt tttccgagca aggacaaggt
     3301 tattgataat gagatgggaa acctagcagg ttatggagct ttgttgaaga aatggtctat
     3361 ccaaattcct cagaagtctt catcttatag tgcattttgt aaaatgatgg aaaattcaga
     3421 ttatagtgca ttttgtaaaa tgatggaaaa ttcagaagaa aattttaaag cgattattga
     3481 tgaagaagtg gggagaaaat aatttcccct ctttctttta tttttaacac aagtaaaacc
     3541 cgaacaatat atttcaattc ataaaataat ttgacaaaaa ctgtactttg gtttataata
     3601 aattaaggaa acgtcggaaa agacgtagtg atgcgagagc ataggtaggt cattacaaaa
     3661 gaaacgagac atcgatatgt taaatgaatt tccaattttt gattacgaag atattcaatt
     3721 gattccaaat aaatgtgtca ttaaaagccg tgcagaagcg gatacaagtg tcactttagg
     3781 aaatcacacc tttaaactac ctgttgtgcc agcgaatatg cagacgattt tggatgaaaa
     3841 tgtagcagag caactggcta aaggtggtta cttctacatt atgcaccgtt ttgatgaggc
     3901 aggacgcatt ccttttatta aacaaatgca cgatcaaggg ctcattgctt ccatctctgt
     3961 cggtgttaag gactatgagt atgattttgt tagccagctc aagactgatg ctcctgagta
     4021 tatcactatt gatattgctc atggtcatgc ggatagcgtg atttctatga ttcaacatat
     4081 caagaaagaa ttgccagata cctttgtcat tgctggaaat gtgggaacac cagaagctgt
     4141 gcgtgaattg gaaaatgctg gtgcggatgc tactaaggtc ggaatcggtc ccggtaaggt
     4201 ttgtatcacc aaggttaaga ctggttttgg tacaggtggt tggcagttgg ctgctctacg
     4261 ctggtgtgcc aaggctgcgc gtaaaccgat tatcgctgat ggaggaattc gtactcacgg
     4321 tgatattgct aagtctatcc gcttcggtgc tagcatgatc atgattggtt ccctctttgc
     4381 aggacacatt gaaagtccag ggaaaacgat tgaagtcgat ggtgaacagt tcaaagaata
     4441 ttatggttca gcctcacaat atcaaaaagg tgcttacaaa aatgtggaag gcaaacgtat
     4501 cttgcttcct gctaaaggtc atctgcaaga cactttaact gagatggaac aagaccttca
     4561 aagtgctatt tcttatgcag gtggacgtca ggttgctgac cttaaacacg ttgattatgt
     4621 gatcgtgaaa aactccatct ggaatgggga tgcttcccac taagacgggg tctttgacca
     4681 aaaaacttgt tattagagca aatttctgtt ataataaaac aagtttccac ccttagtgta
     4741 atggatatca cgtaagattc cggttcttga gatgggggtt cgattccctc agggtggatg
     4801 taaacatcct aaaaagcctt taatagggct tttttcttta tcctgaccca aatttgccgc
     4861 taaaactgag aatctctgtc taaagaattc ccaaatgctt tccagtcccg ttttagagtg
     4921 actcaggggg ctttttttga tataataaaa aggactgtta tcagttagaa agaggttggt
     4981 atgaaagaat tacaaactgt actaaagaat cattttgcaa tcgaatttgc agacaaaaat
     5041 ttactggaaa ctgcctttac tcatacgagt tatgccaatg agcaccgcct cttaaaaatt
     5101 tcacacaatg aacgcttgga atttttagga gacgctgttc tacagttatt gatttcagaa
     5161 tatctatata aaaaatatcc taaaaagcct gaaggtgacc tatcaaaact ccgtgctatg
     5221 attgtccgtg aggagagttt agctggtttt gcgcgtgatt gccagtttga ccagtttatc
     5281 aagttgggta aaggggaaga aaaatcaggt ggtcgcaatc gtgacaccat tcttggtgat
     5341 gcctttgaag cctttcttgg tgcccttctt ttggataagg atgtggccaa ggtcaaggaa
     5401 tttatctatc aagtcatgat tcctaaggtt gaagcaggcg aatttgagat gattacagac
     5461 tataaaaccc atctccaaga gttacttcag gtcaatggtg atgtggctat tcgttatcag
     5521 gtgatttctg aaacagggcc tgctcacgat aaggtctttg atgtagaagt tcttgttgaa
     5581 ggtaagagca tcggtcaagg ccaaggtcgt tctaagaaat tagcagagca ggaagctgcc
     5641 aaaaatgccg ttgagaaggg gctggattca tgtatttaaa ggaaatcgaa attcaggggt
     5701 tcaagtcttt tgctgataag accaaggtcg tttttgacca aggtgtgacg gcagttgttg
     5761 gacccaatgg atctggaaag tccaatatta cagaaagtct gcgttgggct ttgggggagt
     5821 ctagtgtcaa gagtctccgt gggggcaaga tgccggatgt catctttgct ggaacagaaa
     5881 gtcgcaaacc gctcaattat gcttctgtag ttgtgactct ggataatcat gacggattta
     5941 tcaaggatgc aggtcaagaa atcagggtgg aacgccatat ctatcgtagt ggagatagcg
     6001 aatacaagat tgacggcaag aaagtccgtc tgcgtgatat tcatgacctt ttcttggata
     6061 caggtttggg acgagattcc ttctctatta tttcccaagg gaaggttgag gagattttta
     6121 attccaagtc tgaggaacga cgagctattt ttgaagaagc tgctggagtt ttaaaataca
     6181 agactcgcag aaaagaaacc gagagtaaac tgcagcaaac tcaggataat ctggaccgct
     6241 tagaggacat tatctacgag ttggataatc aaatcaagcc tcttgagaag caagctgaga
     6301 atgcccgtaa atttctagac ttagaaggtc aacgcaaggc tatttacttg gatgtattgg
     6361 ttgctcaaat caaggaaaat aaggcagaac tagagtcgac agaagaagag ttggctcagg
     6421 ttcaagaact cttgatgagt tattaccaaa agcgtgaaaa attagaagaa gaaaatcaaa
     6481 ctcttaaaaa gcaacgccaa gatttacagg ctgaaatggc caaagaccaa ggcagtttga
     6541 tggacttgac tagtctgatt agtgatttag aaagaaaatt agccctatcg aaactggagt
     6601 ctgagcaagt ggccttgaat caacaggagg cacaagctcg tttggctgct ttggaggata
     6661 agagaaattc actcagcaaa gaaaagtctg ataaagaaag ctctttagct ctgttagagg
     6721 gaaatctagt ccaaaataat caaaaactca atcgtttaga agctgaattg ctggctttct
     6781 cagacgatcc tgatcagatg attgagctct tacgtgaacg ctttgtagct cttttacaag
     6841 aagaagcgga tgtctcaaac cagttgaccc gtattgagaa tgagttggaa aatagtcgtc
     6901 agctttctca aaaacaagca gatcaactag aaaagctgaa agagcaatta gctatagcta
     6961 aagagaaggc tagtcagcaa aaggaagagc ttgaaactgc caaggagcag gttcagaaat
     7021 tattggctga ctaccaagct attgccaagg agcaagagga gcagaaaact tcctatcaag
     7081 ctcaacaaag ccaactcttt gaccgtctgg ataatctcaa aaacaagcag gccagagctc
     7141 aaagtttgga aaatatcctg agaaatcata gtaactttta tgcaggtgtt aagagtgttc
     7201 tccaagaaaa agatcgccta ggtgggatta ttggtgcagt cagtgagcat ctgacctttg
     7261 atatgcatta tcaaactgcc ctagaaattg cgcttggagc gagtagccaa catatcatcg
     7321 tagaagatga agagtcggca accaaggcta ttgacttcct taaacggaac agagccggtc
     7381 gtgcaacctt tcttcctttg accactatca aggcgcgtac gatttctagt cagaaccaag
     7441 atgctatcac tgtaagccca ggtttccttg ggatggcaga tgagttggtg acttttgata
     7501 ctagactgga agccattttc aagaacttgc tagctacgac ggctatcttt gatgccgtag
     7561 aacatgcgcg tgcagcagct cgccaagttc gttatcaggt tcgtatggtg acattggatg
     7621 ggacagaatt acgcacgggt ggttcctatg cgggtggtgc caatcgccaa aataacagta
     7681 ttttcatcaa gccagaactg gagcaattac aaaaagaaat tgctgcagat gaagcaagct
     7741 tgcgttcaga agaagcggct ttgaagacct tacaagacca gatggctaca ttgacagaaa
     7801 gattagaagt catcaaatct caaggagaac aggcacgtat tcaggagcaa ggcttgtccc
     7861 tcgcttatca gcaaactagt cagcaagttg aagaactgga aactctttgg aaactccaag
     7921 aagaggaaat agatcgtctt tccgagggag attggcaagc ggataaggaa aaatgccaag
     7981 atcgtcttgc tgcaatcgcc agtgacaagc aaaatctgga agctgagatt gaagagatta
     8041 agtctaacaa aaatgccatc caagaacgct atcaaaactt gcaggaagag ctagcgcaag
     8101 ctcgtttgct taagacagaa ctgcaagggc aaaaacgtta tgaagttgct gatattgaac
     8161 gcttaggcaa ggaattggac aatcttgatt ttgaacaaga ggaaatccag cgccttcttc
     8221 aagaaaaggt tgataatctt gagaaggttg atacagaatt gctcagtcaa caggcggaag
     8281 aagccaaaac tcagaaaaca aatctccaac aaggtttgat tcgcaagcag tttgagttgg
     8341 atgatattga aggtcaactg gatgatattg ctagtcattt ggatcaggct cgccaacaga
     8401 atgaagagtg gattcgcaag caaacacgtg ctgaagccaa gaaagaaaag gtcagcgagc
     8461 gcttgcgcta tttacaaagt caattaacag accaatatca gattagctat actgaagctc
     8521 tagaaaaggc gcatgaattg gaaaacctca atctggcaga gcaagaggtg caggatttag
     8581 agaaagctat tcgctcactg gggcctgtca atatagaagc tattgaccag tacgaagaag
     8641 ttcacaatcg tctggatttc ctaaatagcc aacgagatga tattttgtca gcgaaaaatc
     8701 tgctccttga gaccattaca gagatgaatg atgaggtcaa agatcgcttt aaatcaacct
     8761 ttgaggccat tcgtgaatcc tttaaagtga ccttcaaaca gatgtttgga ggaggtcagg
     8821 cagacttaat cttgactgag ggagacttgt tgacagctgg ggttgagatt tctgttcaac
     8881 ctccaggtaa gaaaatccag tcgcttaacc tcatgagtgg tggtgaaaaa gccttatcag
     8941 ctcttgcttt gcttttttct atcattcgag ttaagactat cccatttgtc atcttggatg
     9001 aggtagaggc cgcgctggac gaagccaatg ttaaacgttt tggggattac ctcaatcgat
     9061 ttgacaagga cagccagttt atcgtcgtaa cccaccgtaa gggaaccatg gcagcggctg
     9121 attctatcta tggagtgacc atgcaagaat cgggggtctc aaaaattgtt tcggttaagt
     9181 taaaagattt agaaagtatt gaaggatgac aattaaacta gtagcaacgg atatggacgg
     9241 aaccttccta gatgggaatg gacgctttga tatggatcgt ctcaagtctc tcttggcttc
     9301 ctacaaggaa aaagggattt actttgcggt ggcttcgggt cggggatttc tgtctctgga
     9361 aaaattattt gctggtgttc gtgatgacat tattttcatc gcggaaaatg gcagtttggt
     9421 agagtatcaa ggtcaggact tgtatgaagc gactatgtct cgtgactttt atctggcaac
     9481 ttttgaaaag ctgaaaacgt caccttatgt agatatcaat aaactgctct tgacgggtaa
     9541 gaagggttca tatgttctag atacggttga tgagacctat ttgaaagtga gtcagcatta
     9601 taatgaaaat atccaaaaag tagcgagttt ggaagatatc acagatgaca ttttcaaatt
     9661 tacaaccaac ttcacagaag aaacgctgga agctggagaa acttgggtaa acgataatgt
     9721 ccctggtgtc aaggctatga caactggctt tgaatctatt gatattgttc tggactatgt
     9781 cgataaggga gtggctattg ttgaattagc taaaaaactt ggtatcacaa tggatcaggt
     9841 catggctttt ggagacaatc ttaatgactt acatatgatg caggttgtgg gacatcctgt
     9901 agctcctgaa aatgcacgac ctgaaatttt agaattagca aagactgtga ttggtcacca
     9961 taaggaccag tcggttatag cttatatgga gggcttataa tggcagatat aaaattaatc
    10021 gcattggact tggatggtac cttgctgact actgataaaa ggctgacgga tcgcaccaag
    10081 gcaactttga aagctgcgag tgatcgtggt atcaaggtcg tattgacaac tggtcgtccc
    10141 ttaaaagcca tggacttctt tctccatgag ttagggactg acggccatga agatgagtat
    10201 accattactt ttaatggtgg attagttcag aaaaatacag gagaaatcct tgataaaaca
    10261 gtcttttcat atgatgatgt ggcacgtttg tatgaggaaa cagagaaatt atcactgcct
    10321 cttgatgcca tctcagaagg aacagtttat caaatccaat cggaccaaga aagtctttat
    10381 gccaaattca atccagcttt gacttttgtt ccagttgact ttgaagacct gtctagtcaa
    10441 atgacctaca acaaatgcgt gactgccttt gctcaagaac ccttggatgc agccattcag
    10501 aagatttctc cagaattgtt tgaccaatat gaaatcttta aatcacgtga aatgttgcta
    10561 gaatggtcac caaagaatgt tcataaagca acaggtttgg caaaactaat tagccatctt
    10621 ggaattgatc aaagtcaagt gatggcctgt ggtgacgagg ccaatgacct ctctatgatt
    10681 gaatgggcag ggctcggtgt tgccatgcaa aacgctgttc ctgaagtaaa ggcagccgca
    10741 aatgtagtga cgccgatgac caacgatgag gaagctgtcg cctgggctat cgaagaatat
    10801 gtgctaaagg agaactaaga tatgggatta tttgaccgtc tattcggaaa aaaagaagaa
    10861 cctaaaatcg aagaagttgt aaaagaagct ctggaaaatc ttgatttgtc tgaagatgtt
    10921 gatcctacct tcacagaagt tgaagaagtt tctcaagaag aagctgtgct tgaagaaagt
    10981 caggattcag agccagctgt aggggtttct caagaagaaa tagaagaatt accagaggaa
    11041 gagaagcttg agcacgaagg aactatagaa gaaaataatt ctgaagttct tgaagcagaa
    11101 aggcctcaaa cagaagaaac tgttcaggaa aaatatgacc gcagtcttaa gaaaactcgc
    11161 acaggtttcg gtgcccgctt gaatgccttc tttgctaact tccgctctgt tgacgaagaa
    11221 tttttcgagg aactggaaga actgttgatt atgagtgatg ttggtgtcca agtcgcttct
    11281 aacttaacgg aggaactacg ttacgaagcc aagcttgaaa atgccaagaa acctgatgca
    11341 cttcgtcgtg tcatcattga gaaattggtt gagctttatg aaaaggatgg tagctacgat
    11401 gaaagcatcc acttccaaga taacttgaca gttatgctct ttgttggtgt gaatggtgtt
    11461 gggaaaacaa cttctatcgg aaaactagcc caccgctaca aacgagctgg taagaaggtc
    11521 atgctggttg cagcagatac cttccgtgcg ggtgcagtag ctcagctagc tgaatggggc
    11581 cgacgagtag atgttccagt agtaactgga cctgaaaaag ctgatccagc cagcgtggtc
    11641 tttgatggta tggaacgtgc cgtggctgaa ggtatcgata ttctcatgat tgatactgct
    11701 ggtcgtctgc aaaataagga taaccttatg actgagttgg aaaagattgg tcgtattatc
    11761 aaacgtgttg tgccagaagc accacatgaa accttcttgg cacttgatgc atcaacaggt
    11821 caaaatgccc tagtacaggc caaagaattt tcgaaaatca cacctttaac gggaattgtt
    11881 ttgactaaga ttgatggaac tgctcgagga ggtgtggttc tagccattcg tgaagaactc
    11941 aatattcctg taaaattgat tggttttggt gaaaaaatcg atgatattgg agagtttaac
    12001 tcagaaaact tcatgaaagg tctcttggaa ggtttaatct aatcagaagc aaaaatcctg
    12061 caaggcataa acttgcagga aattttttta ttcgaaacga ccatcttgac gataggcgat
    12121 atctggttgc caagtccatt tggcaccgaa tttttcaagt aggtcaaagc tggcttgagg
    12181 tcccatgctt ccagctttat agtcatgaag tggggcacca ttttcagccc agagctcttc
    12241 aatacggtca atcaacttcc atgatgcacc aacttcatcc cagtggctaa agttagttga
    12301 gttgttattt aggacatcat aaatcaattt ctcgtatggt tctggagaag caccagttgc
    12361 agtcgcatct gtacggtaat caagtgagtt aggagccaag ttaaattctt ctcctacttg
    12421 cttcccattt aggctaagag agaagccttc tgttggttga atatagatgg tcaaaatatt
    12481 tggagcaagt ggttctccaa agatagaatc catttgttta aagacgatgt tgacatgagt
    12541 tcctttttca gtcagtcgtt tacctgtacg gaaaaagaaa ggaacactac ggaatcgatc
    12601 gctgtctaca aagaaggcac cagatgtaaa ggtttcagtt gttgattctg gattcacatt
    12661 tggctcgcta cgataagaga tgtatttcat gccatcaatc ttaccagagc ggtattgccc
    12721 acggataaag tgttctttga gttcttcatc agttggatga tagaggtttt taaagacctt
    12781 aatcttttca gcacgaatct cgtcttttgt gaagattgct ggtttgtcca tggcgaggag
    12841 cgaaagaagt tgtagagtgt ggttttggac catgtcacgg agggcaccgg attggtcata
    12901 gtagccacca cgttcttcta cacccaagcg ctccgcaaag gtaatttgaa cattgtcgat
    12961 aaaatccttg ttccaaacgt tttcaaaaat caagtttgca aagcgaactg caaagatgct
    13021 ttggatcatt tccttaccaa gataatggtc gatacggaaa atttgttctt cgtcaaatgt
    13081 tgctaggagt tcgtcattca acttgcttgc agttgcgtaa tctgtaccaa atggtttttc
    13141 aacgatcaag cgctcaaaac ctttgccatc gacaatgttt tcagatttga ggtgtttggc
    13201 aatggttcca aagaactgag gtgccataga caagaagaag agcttattgt gttcagcttg
    13261 gtatttttca ttaagctcag cttgtaattg acgcaaagca atataatgtt ccgaatcatt
    13321 gacatcatgg ctttgatagt agaagtggct agcaaattct tgggcttgct cggtactatc
    13381 tgccaaatca aggatggact cgacaactac agattcaaaa tattccttac tccaaggtct
    13441 acgggcagtt ccaataacgg caaagtgctt ggaaagattg ccggattgat atagtctaaa
    13501 aagggaaggg tagagcttgc gtttagccag gtctccactc gcaccgaaaa ttgtaacaat
    13561 aaccttagat gacatctagc tacctaattt ctatttttat tcctagtctt gctaggtatg
    13621 aggaaacctc atttcataaa cttaattcta acataatttt ccgaaaaatc aaaaattcca
    13681 atacgaggta gaaaaactgc aaggaaatcc ttacagtttg agtttagacg tttaagacct
    13741 tatctaagaa ctctttcaga cgagggtgtt gtgggttatc aaagatttgg tcaggtgttc
    13801 cgtcttcaag gaactcgcca tctgcagtaa agataacgcg gttggcaacc tgacgagcaa
    13861 atcccatctc atgggttacg ataatcatgg tcatgccttg ctcagccaat tccttcataa
    13921 cgttaagtac gtctccaacc atctcagggt caagggcaga agttggttca tcgaagagca
    13981 tgatgtctgg attcattgct aggccacgag cgatggccac acgttgtttt tgaccacctg
    14041 ataggttatc tggattggca ttagctttat ctgctagtcc aaccttttca agcaactcca
    14101 ttcccaattc ctcagcttct tccttagtca tcaacttgtg ctcaatagga gcaaaggtga
    14161 tgttgtccaa tacagacata tgagggaaga ggttgaagtg ttggaatacc atgccgatat
    14221 tttcacggac gtggtcaaca ttggttgttt tttcagttaa atcatagccg ttcacagtga
    14281 tgtgaccgct agtgacttct tctaaaagat tgaggctacg gaggaaagtt gacttaccag
    14341 aacctgaagg accgatgata caaacaacat ctccttcata gaacttagtc gtaattcctt
    14401 ttaggacttc attttttcca tagtgcttgt gtaaatcatt tacatcaatt tttaattttg
    14461 ccattaacga atcctctttt ctaagcgttt cgctagtcta gtcaaaagtg tgataattac
    14521 aagatagaag atagcaagga ttgcatacat cttgaaactt tggtagttac gagcaatgat
    14581 aatcttacca gtttggaaga gttcaaccaa accgatagca gatacgatag ttgtatcttt
    14641 aagagcgata acgaattggt tgacaaagtt tggcaacatc aatttagttg cttgtggcaa
    14701 gataatctta cgcatggttt ttccataaga gatacccaag cttcggctgg cttccatttg
    14761 gccaactgga acggcctgaa taccaccacg aacgatttca gcgatataag cagccgcatt
    14821 gagtgagagg gcaatggttc cagctacaaa gtcgttaatt gggctttgtt ggcctgtgat
    14881 agactcgatg aagtttggaa ttccccagaa gatgaaggct gcaagaatca tcaatggaat
    14941 accacgaata acgtcaacga aaatctcaga gatgacgcga agagatttgt atgggctaac
    15001 gctaaacata ccgaagataa ttccgatgac aatggcaata gcaaatgaga taagagctag
    15061 agcaagagtg ataccaagac cgctaaggag ttgtttgtag ttgttttgaa gcaagcccca
    15121 gagcgttgtt tcgtcaacag tacttgttga agcagttgaa gattcgctag ctaggtattt
    15181 gtcaagaatc ttttggaatt caccgtttgc tttaaggttt gcaagtccgt tgttgaacat
    15241 ttcaatcagt tctggatttg ctcctttttt aacggcaaag gctgtttcac cgattggagt
    15301 tccagagatt ggagttttca atttttgacc ttggctgata gaatatttga gaacaggttc
    15361 atcatccata acggcatcaa tggcaccagt gtttaaactg tcatacattg aagaaccatc
    15421 agcaaaggtt ttgattttgt aaccgtattt gctttgattt tctgttagga aggtttgaga
    15481 agcagttccg tttttaacac cgactgtctt tccttttaga tcttcataag aagcaatatt
    15541 gcttgattct ttgacaccaa gaatggtatt agcagtgtag tatgattctg agaagtcaaa
    15601 agttgcctta cgagcatctg tgacagacat accagcaatg ataccatcgg cttgaccagc
    15661 ttggacagca ctgatagcag catcaaaacc agggttggtg atttcaattt caaaaccttg
    15721 gtctttagcg attgccttaa tcaattccat atcaatacca gtgtattggt tgcttgaatt
    15781 ttggaaaaca aaaggtgcaa aagaagaatc gctggcaatg atatatttag ccttaacagg
    15841 aatagctttt aatcctgcga gagtagttgt agttggcact gctgaagatg atgaagcagt
    15901 ccatttcttg ataattttat caagactacc atcttttttc atttcagcca aggcttggtt
    15961 aaattcagta accaggtgct cgtatttact tccttttttc acaccgaaag caaaacttcc
    16021 tacagcttca ccatccattt caatatggag gtcttgacct tggttaatgg catattcgat
    16081 aacaggtttg tcatccatca tggcatcgat ggcaccagca ctcaagctgt tgttcattaa
    16141 atcaccagtg tcaaatgttt taatagtaaa gccgtattta tctttgattg tttcaaggaa
    16201 acgttgagcg gcagttccgt ttttaacacc aacggttttg ccagttaatt ggtcgtactt
    16261 gctaattttg tgtgactttg tagtagcaat gacaactttt gtatcatagt aagtatcaga
    16321 catggtgaag actttttcac gttctttagt ctttgtcatc cctgccatga tagcgtcggc
    16381 ttgcccagct tgaaccgcat tgactgctgc gtcaaatcca ggataggaca tctgaatgtt
    16441 ccagccttta atctcagcga ctttgttaat aatgtcaaca tcaattcctt tataagtttg
    16501 atctgaatct ttaaactcaa aaggtgcata ggcggtatca gaaacaatct taatcgtttc
    16561 tgctttggca atacctaatg agaaaattgg gaataaaatt agcaaaaatg ctagaaattt
    16621 tttcttcatt tttagtctcc ttttccgaat attctctcat tatatcagaa aaagtaaaaa
    16681 aaggcaaatt tttatatttt tcgtgtcaga aaatataaca ttgatttttc cttttctttc
    16741 ttgaattgct attaaaaatt gctataataa tactatatat agaagaaaag aggacgggga
    16801 tatgaagaac aaaagaatat ttaaagactt ccaagcttca aaaatgaatt taaaccttta
    16861 cacaagcccc ttgttagcct ttgtttttgt cttcatagga gagtttgtgg cttatacttt
    16921 atatggtatt agtttgttag ctctcatcgg acttgctaga aattttggag aggctggtca
    16981 aaatcttgca agctacttgc agaccttgca tcagagcttg acggataaaa caagtgactt
    17041 tcgtttaatt ttaggattac tggcctttgg ttttattctt aacactgtgt tcagatggac
    17101 aagaaaagtt gagaaaagac ctattcgaac cttgggattt tatagagaga attgcctcag
    17161 caatcttctg aaaggattta gtctaggcct ggcacttttt cttctgacct tgttaagttt
    17221 agtggtctta ggtcaatatc gtttggaatc cattcacttg aatccttatt atcttgcctt
    17281 tgtcgtcttt actatcccat tttggatttt acaggggaca gcagaagaag tggtggcccg
    17341 tgcttggcta cttcctcaat tggcctcaag aaccaatcta aaactagcta ttcttatatc
    17401 tagcctgttc tttaccctgc ttcatatggg caattctggc ctcacccctc tatctctagt
    17461 aaatctcttt ttattcggag ttgccatggc tctttacctt ctcaaaactg atacagtttg
    17521 gggtgttgca ggtattcatg gtgcttggaa ttttgctcag ggtaatctct ttgggatttt
    17581 agttagtggt caaccgtcag gaacgtctct gatgaccttt ttaccacaag gcaatcaaga
    17641 ttggctatca ggtggttctt ttggcataga aggttccatt atgacaagtc tggtattact
    17701 actgctgatt gtctatcttg ctaataaatt aaagaaagaa aatgaaagga tgtgacttcg
    17761 gtccgtcctt ttcttcgtga aaatactata agtatgctaa aataggaata gcacatggag
    17821 agaggattct tatgatcaat cacattacag ataatcaatt taaactagta tcaaaatatc
    17881 aaccatcagg agatcaaccc caagctatcg agcagttggt ggataacatt gaggggggag
    17941 aaaaagctca gattctgatg ggggcgactg gaacagggaa gacctatact atgagtcagg
    18001 tcatttctaa agtcaataaa ccaactctgg ttattgccca caataaaact ctggctggtc
    18061 agctctatgg ggagtttaag gaatttttcc ctgaaaatgc agttgagtat ttcgtatcct
    18121 actatgatta ttaccagcca gaggcctatg tcccttctag cgatacctat attgagaagg
    18181 atagttctgt caatgacgag attgacaagc ttcgccactc agctacctca gcccttttgg
    18241 agcgtaatga tgttattgtc gtggcctcag tctcttgtat ctatggtttg ggttcgccca
    18301 aggaatacgc tgatagtgtc gttagtctcc gtcctggtct agagatttct cgtgataaac
    18361 tcttgaatga cttggtcgat attcagtttg aacgcaatga tattgatttc caacgcggaa
    18421 gatttcgcgt tcgtggggat gtggtagaga ttttcccagc ttcccgagat gaacatgcct
    18481 ttcgagtaga attttttgga gacgaaattg accgtattcg tgaagttgag gctctgacag
    18541 gtcaggtgtt gggagaagtg gatcatttag cgattttccc agcgacacac tttgtgacca
    18601 atgacgacca catggaagtt gccattgcaa agattcaggc cgagttggaa gaacaattag
    18661 ctgtctttga aaaggaaggt aaactgcttg aagcccagcg tttgaaacag cggacagagt
    18721 atgatatcga aatgttgcgt gagatgggct ataccaatgg ggttgaaaat tattctcgcc
    18781 acatggatgg acggagcgaa ggagagcctc cttatacgct tctcgacttc ttcccagatg
    18841 atttcttgat tatgattgac gagagtcata tgaccatagg gcaaatcaag ggcatgtaca
    18901 atggagaccg ttcgcgtaaa gaaatgctgg ttaattatgg tttccgtttg ccgtctgctt
    18961 tggacaatcg tcctctccgt cgggaggagt ttgagagtca cgttcatcag attgtttacg
    19021 tttcagcgac acctggtgac tatgaaaatg aacagaccga gacagtgatt gagcaaatca
    19081 ttcgtccaac gggactcttg gatccagagg tggaagtccg tccgactatg ggacagattg
    19141 atgacctctt gggtgaaatc aatgcccgcg ttgaaaaaaa tgagcgtacc tttatcacaa
    19201 ctttgaccaa gaaaatggca gaggacttga ccgactactt caaggaaatg ggtatcaagg
    19261 ttaagtacat gcactcggat atcaagacct tggaacggac ggagattatc cgtgacctgc
    19321 gcttgggtgt ctttgatgtc ttggtcggaa ttaacctgct ccgtgaagga attgacgttc
    19381 ctgaagtgag cctcgtagct attctcgatg ctgacaagga aggtttcctt cgcaacgaac
    19441 gtggactcat ccagactatt ggacgtgctg ctcgtaacag tgaaggccat gtcatcatgt
    19501 atgcagacac ggttacccag tctatgcaac gtgctatcga tgaaactgcc cgccgtcgga
    19561 aaattcagat ggcctataat gaagagcatg gtatcgtacc acagacaatc aagaaagaaa
    19621 tccgtgacct gattgctgtg accaaggcag ttgctaagga agaggacaag gaagtcgaca
    19681 tcaatagcct caacaaacaa gagcgcaaag aactcgtcaa gaaactagaa aaacaaatgc
    19741 aagaagccgt cgaagtactt gactttgaac tggcggctca gatccgtgat atgatgttgg
    19801 aagtcaaggc cttggattag gggaatagta tgatttattt aagaaagtta aagagagaag
    19861 atttgatgtc tttatgggaa atggcttatt cacaacttaa tccagtttgg aaacagtatg
    19921 atgctcccta ttatgatgat tatcagtatt tttcaaattt taaagaattc gaactacaaa
    19981 aatcagaatc cattttaagc aactcaaatc gccttggtat ttttgttgat gataaactag
    20041 ttgggactgt ttcgcgttat tgggtatgta aacaaacaag atggatggaa ttgggaattg
    20101 gtatttatga taaaaaattc tggaacactg gtattgggaa agttgctatg ttgcagtgga
    20161 tagataggac gtttcaggat tacttggagt tggagcatct gggtttgaca acttggtcag
    20221 ggaatcttgg tatgatgaaa cttgctgaaa aattaagaat gaaaaaagaa gctcatattc
    20281 caaaagttcg ttattatcaa ggtaaatatt ttgatagtat taaatatggt attttgagag
    20341 aagactggga gaaaacaaat gacggttatt atcaaatcaa tggaaactcc tgaagagata
    20401 gaaggtaagc cccttgttca ctggcaaacg tggagagagg cttatgatgg ccttttgcct
    20461 gcagaatttc aggagacaat gacattagaa agatgtcgat tctttagtca aaagtatcca
    20521 gaaaatacat tgattgcgat ggatggtatg aaggtagttg gttttatcag ttatggcaac
    20581 tttcgtgatg agactattca agctggcgaa attattgctt tatatgtttt aaaagactat
    20641 tatggaaaag gtatcgcaca aaagttaata aaagcagctt tgattgctct taatcatttt
    20701 tctgaaattt tcttatgggt attgaaagat aataagcgcg caattgcttt ctatcaaaaa
    20761 atgggcttta cttttgatga acaagaaaag atacttgaac ttggaaagcc cataaaggaa
    20821 aaacggatgg tattctattc taaataattt taaaaagtag aagctaatgg tactggtatc
    20881 aagtctaaaa atttaataaa ttagaaagtg agtaaattta tgtctcgagc acaattaacg
    20941 attttaacaa atatttgtct gattgaagat ctcgaagctc agcgcgtggt gatgcagtat
    21001 cgcgctcctg aaactaatcg ctggtctggt tatgcctttc caggaggcca tgtagaaaat
    21061 ggcgaatctt tcgcagagtc tgtcattcgt gaaatctatg aagaaacagg gttgactatc
    21121 caaaatcctc aacttgtcgg cattaaaaat tggccactag atacaggtgg gcgctatatt
    21181 gtcgtttgtt ataaggcaac tgagttctct ggtacccttc gctcttcaga tgaaggagaa
    21241 gtttcttggg tgcaaaaaga ccagattcca aacttggatc tggcctatga tatgctacct
    21301 ttgatggaga tgatggaagc atcggacaaa tctgaatttt tctaccctcg ccgtacagaa
    21361 gacgattggg aaaagaaaat tttctaatcc tttactaaat aacctagctg atccaaggcc
    21421 tcctcgatat agtggaggtc ttgttgtgtt tcggcttcga ctaggtggta atggacacca
    21481 tctgtcaatt cagataaagg tttaaaatca gaatgctcga cttgttctag aaaatgttga
    21541 acgtctcggc ggcaagacag tttaagcaag gtttcaattt ctccataaac gggatggtca
    21601 atcaaagtat tctggacatg cccaccatta tcgacaatag caaggagttc ctgaccgatt
    21661 tcttcaactt catgtttcac tttgaaaagt ttgtgaacat aaggattggt atcaatttgc
    21721 ttatagatgt agccacgatt ggtggatagg ataggagcgc catcggctct caaaattgca
    21781 atgtcctgca caatgacctg acgtgtgaca tgaaaatgtt ccgccaaact ttggccatta
    21841 agggctttag gagcttcttt caagagttgg agcagggctt gtttgcgatc ttttgtcata
    21901 gcttttcctt ttaacggcgt tttcgaagca ctttatagac agctagtgct aatgtatagt
    21961 ctaccatact atggataatt gtgccaaatc caactagtac aaatagaaca tagaacatgt
    22021 tttctacatt ggtacccgaa gttgcgtaaa aaaggacaca ggctaatact tcagcaaggg
    22081 catgaacaac agccaaaaca aaattgaaaa cccaggaaga ttttggttta tctagggtat
    22141 cggggaattt ttgtaggtaa agagatccta aagcaccaaa agatatatgg gaaaaagccc
    22201 gaaaaactat aaccatggga tagccagcca tcaaaaatcc aaaactagag gctaggatga
    22261 caaaaactgc catcaagggc gacaagaaca tggctataaa aatagcgatg tggctcccca
    22321 aagtatagga agcaggtgga atgacaatct tgaaaggcat aacaattgga atcaaaatcg
    22381 caatagccgt taaaagggct gtcattgtca taaattgtgt ctttttccgt gtattcacaa
    22441 gaatctcctt tttaactgca tatacactag tatggtacaa taaaccagac aataaagcaa
    22501 gaatttactt gggtttatag atcatttttt agttaaaagt tatagtagat ggaaactaga
    22561 atagtccacc tctacttcta aaacattgtt ag
//
LOCUS       DAVBHS010000014        22406 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVBHS010000014 DAVBHS010000000
VERSION     DAVBHS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22406
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.014"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(48..272)
                     /locus_tag="VQ651_000396"
     CDS             complement(48..272)
                     /locus_tag="VQ651_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HET2753439.1"
                     /translation="MIEMLIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFVLLLTFMVITYK"
     gene            complement(277..456)
                     /gene="asp4"
                     /locus_tag="VQ651_000397"
     CDS             complement(277..456)
                     /gene="asp4"
                     /locus_tag="VQ651_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HET2753440.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(458..1795)
                     /gene="gtfB"
                     /locus_tag="VQ651_000398"
     CDS             complement(458..1795)
                     /gene="gtfB"
                     /locus_tag="VQ651_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HET2753441.1"
                     /translation="MIELYDSYSQESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYVDGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGACFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHHPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIVEALPDVTFRIAAVTEMSSKLLDMLRYPNVVLY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPEH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQEIMQTVLGG"
     gene            complement(1788..3299)
                     /gene="gtfA"
                     /locus_tag="VQ651_000399"
     CDS             complement(1788..3299)
                     /gene="gtfA"
                     /locus_tag="VQ651_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HET2753442.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLREIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(3311..5683)
                     /gene="secA2"
                     /locus_tag="VQ651_000400"
     CDS             complement(3311..5683)
                     /gene="secA2"
                     /locus_tag="VQ651_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HET2753443.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSSLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKQYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDIVYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(5676..6116)
                     /gene="asp3"
                     /locus_tag="VQ651_000401"
     CDS             complement(5676..6116)
                     /gene="asp3"
                     /locus_tag="VQ651_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HET2753444.1"
                     /translation="MIITQRQSIYWGEVGGTYMYGTTVSYYLDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKAKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(6113..7648)
                     /gene="asp2"
                     /locus_tag="VQ651_000402"
     CDS             complement(6113..7648)
                     /gene="asp2"
                     /locus_tag="VQ651_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HET2753445.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKTLREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(7632..9212)
                     /gene="asp1"
                     /locus_tag="VQ651_000403"
     CDS             complement(7632..9212)
                     /gene="asp1"
                     /locus_tag="VQ651_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HET2753446.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFEPNGQIHMHHIMDDRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNPQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLTDVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(9222..10439)
                     /gene="secY2"
                     /locus_tag="VQ651_000404"
     CDS             complement(9222..10439)
                     /gene="secY2"
                     /locus_tag="VQ651_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HET2753447.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRITLPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSFSKRLGLTSTSIEIQDRR
                     KMYLTLMIAVIQSLAVSLRLPVQSSYSAILVVLMNTILLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSMVLNIPQDVLETFQTVHIPTGIIVLLALLTLVFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYFYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMIGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVVMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYKPLI"
     gene            complement(10496..11173)
                     /locus_tag="VQ651_000405"
     CDS             complement(10496..11173)
                     /locus_tag="VQ651_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET2753448.1"
                     /translation="MGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
                     YNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFN
                     EVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVE
                     VFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDA
                     EQRNGKS"
     gene            complement(11158..11400)
                     /locus_tag="VQ651_000406"
     CDS             complement(11158..11400)
                     /locus_tag="VQ651_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2753449.1"
                     /translation="MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTD
                     NSGEICDAFMMQDNRVRVLHQENKGGQHKLKIWGLV"
     gene            complement(11400..12596)
                     /locus_tag="VQ651_000407"
     CDS             complement(11400..12596)
                     /locus_tag="VQ651_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HET2753450.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNAGMLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGVDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            complement(12605..13825)
                     /locus_tag="VQ651_000408"
     CDS             complement(12605..13825)
                     /locus_tag="VQ651_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET2753451.1"
                     /translation="MRNTKRAVAFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(13856..16300)
                     /locus_tag="VQ651_000409"
     CDS             complement(13856..16300)
                     /locus_tag="VQ651_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HET2753452.1"
                     /translation="MIKTIVLAGDRNYIRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYTRYFIADYIQEDKVLYLD
                     SDLIVNTSLEKLFSICLEEKSLAAVKDTDGITFNAGVLLINNKKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELDKEFNLQVGHDVTAFYNKWENYFNE
                     LVPPSIIHFVSYRKPWTTLIANRYRDLWWEFHDLEWTKILQHHIGEFELTSSLDKEFS
                     CLTLTNSQDLEGIEELVTALPDVVFHIAAWTDMGDKLIKLAVYDNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQDIEQLDYLAGQLPNVVFQVAAWTAMGPKL
                     YDLSNRYPNIQLYPAISRDKLDELKEKMDAYLDINLLTSTSDIVAEMAHLSKPILAFY
                     KSQNGNNGQRLYSSEHPERMLADLQKLITKDMLEKPLDIIQVKGIDETLDYIIEHNSS
                     LVRFGDGEINMLAGHSIPYQDYDEELVSIMRDIIGQESREDLVVCLPDAFTDRFRFTS
                     WAIPFWKDHMDHYMDFYRELCSDSWYGSTFVSRPYIDFEDKSQAKAQFEKLKSIWENR
                     DLLIVEGATSRSGVGNDLFDEANSIKRIICPSHSAFSRVHEIEQEIEKYAGGRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHIDSEYEWMKMGAKTKVKFSHKHTAEYNFDQDIEF
                     IDDETYNSQIVARILN"
     gene            complement(16310..17320)
                     /locus_tag="VQ651_000410"
     CDS             complement(16310..17320)
                     /locus_tag="VQ651_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET2753453.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYMYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSSARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTYQNDGESNQYYTLNISHKVSTYLTAGIPVIVPSSLSTAKFIV
                     DQGLGFVANSLEEVHAIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     VE"
     gene            complement(17349..18224)
                     /locus_tag="VQ651_000411"
     CDS             complement(17349..18224)
                     /locus_tag="VQ651_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2753454.1"
                     /translation="MENELISIVVPIYNVENYLRQCLDSIQKQTYPNFECIMVNDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGAYITFVDSDDWLEHDALD
                     RLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDDSLEVIEGKAIMDREGVEEVRN
                     GNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVLLRASRIVYLNRCVYWYRVGLS
                     DTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHILIYKNRLQRVIAKLEEQNMQF
                     TEIYRRMMEKLSLLP"
     gene            complement(18264..19142)
                     /locus_tag="VQ651_000412"
     CDS             complement(18264..19142)
                     /locus_tag="VQ651_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HET2753455.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLESLPNICFYIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLVSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVICRNFENFHVSSGKLILHDGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVTIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLVVKRRD"
     gene            complement(19139..21224)
                     /locus_tag="VQ651_000413"
                     /pseudo
     CDS             complement(19139..21224)
                     /locus_tag="VQ651_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(21285..>22406)
                     /locus_tag="VQ651_000414"
     CDS             complement(21285..>22406)
                     /locus_tag="VQ651_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2753456.1"
                     /translation="STSASASASTSASESASTSASTSASASASTSASASASTSASTST
                     STSASTSASTSASTSASTSASTSASESASTSASTSASTSASASASISASESASTSASA
                     SASTSASASASTSASESASTSASASASTSTSASESASTSASASASTSASASASTSASA
                     SASTSASESASTSTSASESASTSASASASTSASASASTSASASASTSASASASTSASA
                     SASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASA
                     STSASASASTSASESASTSASESASTSASASASTSVSNSANHSNSQVGNASGSTGKSQ
                     KELPNTGTESSIGSVLLGVLAAVTGIGLVAKRRKRDEEE"
BASE COUNT         7085 a         4776 c         3588 g         6957 t
ORIGIN      
        1 gaaaacttca gaccaatcca caaaaggtga atatctgaag ttttctttta tttataagta
       61 atcaccataa aggttaatag tagaacaaat aaagccaaac tcaccaataa agtgagcacc
      121 ttgaccaagg tgttgctctg ccagtagctt ggtttaccaa tattactagt ggcatccatg
      181 gaaaatagtt gattttgacg gggttgtata gttaccaata caatcaaagc aagagatagg
      241 ataatagcta aaacaattag catttcaatc attgatctac ctcaaaattc ccaacaaagt
      301 aaagagcaaa ccaatcagaa tcattaagcc cagtaaaagt gaaaaagttt tactaatctt
      361 ctcccctcgg gtttcttttt ccttcttgat gggtttttgt ttcaactctt ccatgcgacc
      421 ttcaacgtct ttgtaaaata aatcttcctc tgacatgtta gcctcctaaa acagtttgca
      481 ttatttcttg atatcgcacc aagtcaacat aatttgcatg ttggccttgt ttgccaagtg
      541 cctgacgcat ttgatcaaca tctgaaaggg ccaatttaat ggtctcaacc aaggcagcaa
      601 cttcactact ttcaaataga tgttctggag cgatataaag tctattgtga accgtttggt
      661 taaatccaag aatcaagaga ttgtgctcaa aggcctgacg cactgcctgc aacaactcat
      721 tgctgtgatt gatatccaag taaatatccg acagttgata cagctcctga atcttctgtg
      781 gactagcatt ctggtaaagg accacattag gatagcgaag catgtctaag agcttagaag
      841 acatctctgt cactgctgcg atacggaagg taacatcagg caaggcttct acgattgctt
      901 ctacttgctc aatctgatct gaattggtca agattaaggc atctcgtctt aggaaattat
      961 cacgtttgaa ctggtaatga taacccaagt gcacaaactg atcatggtat ttctcgtcag
     1021 ttaactctaa agcgcgctca taagtcgcct tatttggaat gatgatcttc ttagtacgca
     1081 cattatcact ttccaaaatc aactgcatat tacctggaat ggcatcatag agaggttcct
     1141 gccataccaa gacatccgag ccagatttat ctggatgatg gaaggaaacc aagaaaggag
     1201 tcgctagagt attaaagata agctgactgg tatctatttc caaatcttgc aaaaagaagg
     1261 tgataaattc aactttattt gcaaagtaac gcatggactg accaggcaaa gtcaataaga
     1321 tatcacccgt cacatggttt tccagtaaaa cttgttggcc attgacatct tggtaaactg
     1381 tcataatcgg ctcgctatct gcgctataag tggttttagc aaagcaagca ccaaagcgat
     1441 tgtagtggtc aacctgacgt actcgacctt ctaggtcttt ccagtctacc tgtttaacca
     1501 agcgagcctg cattccatca acataatgaa tgacagccct ctcctgcgtc acatcttcaa
     1561 tacaagcaga ctgattatct cctaaaattt cccaaaaatc tgaaacggga acttggttaa
     1621 aatagagagg ttttccatcc tcgtaaccta gataataggt aaaaggagaa agcagaccat
     1681 caggcagaaa accatctgca tcgatgacca ctccaagttg agaaagacca gtagcgacta
     1741 gactttcatg taaatctcta ctttcttgac tgtaactatc ataaagttca atcatggagc
     1801 acctcctcta ctgttttctt ccacttttct aaaatttctt tggtcaagaa gccttctgca
     1861 atttgataag aataggcacg catagcttcc aaacgatttt cttgatacaa ttgacaaatc
     1921 ttagcggcat aagcttgctt gatttggtct tctacatggt cagatgaact tggaatcaaa
     1981 taaccatttt gcccatcctc tataaaggtc tgattaccat aaggcacatc aaaaccaatt
     2041 agaggtagac ctgaaccaat agcttccatc aaggtcagac caaatccttc gctggtagaa
     2101 gccgttaagt agacctcata ctggctataa atctgcgaaa gttccgcatg ccccttgagt
     2161 tggatatagt cctctgcctg atgatttgca ataatttctc taagcagaga atcttctcca
     2221 ccactaccat agatatcaaa ggttagttcc ggtaactcct tatgagcttc aatcacagct
     2281 ttcacaagcc aatcaatgtg cttttctttg gcaagacgtg aagccgtaat caatgaaaat
     2341 ggtttgcgcc cttgacttga atctgtcaag gaatcaatac tgcctacagg aatggtaaca
     2401 atctttggct gatgctgagt atatttggca aattgctctt gtagaacttc attttgtcta
     2461 tcagtagaca cgataaagaa gtcaacctta tctgcattgg taaactgata gtcataatag
     2521 ttattccaaa ggatatagtc ctcatttgta gcattttcac tataatgctc cgcatgaaca
     2581 actaccgcta gatgtgctgt ctgtgcttcc tcaaacacaa cctgtccaat acctgtctcc
     2641 ctatccagaa taaccaaatc agacttgttc aaattcaaag atttcataaa ggcacgcaca
     2701 aaagcttgct ttccatagaa aatcttatcc ttgaaatgat aaacttcttc cttcccttga
     2761 ttcatcaaaa tatcatagac tggagtcccg tcttcattgt aaaaagttcg ttggtataag
     2821 actgcaacat tgtccttggg agcaaaatac tcgctacaat aacgcgtata agaaaagtaa
     2881 tccttccgaa tcaggtttcc cttaaaaaca tactcggcat gttgaaccaa gtccttgttc
     2941 tcatcaacca aataacaggt tacaaactta tcttggtcaa aaaagaatac acgtaaaacc
     3001 ttgccatttt tttctctgtg actttcttca ccaccaaagt aagccaagac atcatccact
     3061 gtcacgctag taggtgcaat tttgatatct gtgaaatgat tataaagcca gataacctga
     3121 ttatcatcaa aaccaatatt ggctgttaag tgctgaatat tatcggctaa aatcatatct
     3181 gtaaagataa acttagagga cagatttaat ttccgaaaaa caccagcacg ataggcttga
     3241 gcgtattcaa caccgctact agcccaacca attcctaaat ttatattgta aattgtcata
     3301 ttttctcctc ttatggaaaa tgagtcacga tttcaccttt tggagtgacc tcaaccagcc
     3361 ccatcaggag attacgcacc atatccgcac ggatttgttc tttcatagct tcaaagcccg
     3421 cgtaggcttc ttgatagtac tctacgattg gatttttctg actagcagat tgaccaccga
     3481 tagccatgga tagctgttgt agatagtcta cctgctctac ccagttgtca tcaatggctt
     3541 taagcagtga aagtcgtaaa aactgttcat ataagtcatg ttgattaagt aattctttct
     3601 tttcagaaag ttctttatca atcacctgct tcataaagct acgaactgca gttttgtcag
     3661 ttacatctat ataatctgga acctctttaa catgaaaact aatattggtc acaataaagt
     3721 gaaacaataa ttcacgacta gcatagtgat cagccgctac ctcttctgta tatctctcaa
     3781 tgatatccac aacaacatcc tctaagtcac gagaaccatc tattagacga tttctctctt
     3841 tatagactat atcccgttgt atattcatac tttcagcata ctccagagtc tgacgacgtg
     3901 ctgaacgtcc agcactatca ctggcatgct gagccttttc gactagtttc cggtatttac
     3961 gacctttcaa tacttccggt tgagtcatat cttgaacctg atagtctttg tactttttat
     4021 gcacccaaga tggaccaaat ttcttgataa catcatcctc taaggataca aaaaatttac
     4081 tcataccagg atctccctga cgaccagaac ggccacgaat ttgtaggtcg atccgctgac
     4141 tttccatccg ctcagtccca ataacaatca agcccccaag ctctgcgact cctttaccaa
     4201 gcttgatatc cgtaccacgt cctgccatag aggtagccac tgtcacagcc cccatctgac
     4261 ctgactcgga gataatctga gcctcacgcg ccgcattatt agcatttagg acattatggg
     4321 caatcccttc acgaaacaag agagacgaat agagttgaga catttcaact gagcctacaa
     4381 aaacgagtaa aggatttccc ttagcatggt attgcttgat gtactccaag gatgcataca
     4441 ctttttcagg taaagtgata tatagattat ctggatagtc aatccgttgt ctcggacgat
     4501 tggttggaat gcgtactaca gacatattgt aagtttcaat aaactctttt tccgcgacct
     4561 tacctgtccc tgtcatacca gatatcttat taaacatctt aaaaagactc tgataggtga
     4621 tcgaggccat agcccgcgtc tcaggagata atttgacatg ttccttggct tcaatagcct
     4681 gatggagacc tccttgaagt ttagtcattt ccattagacg ccctgttccc ttatcaacca
     4741 gtaccatctc atttccacga atgatatagt ccttatcttt agtaaagagc ttatgagctc
     4801 gaatcgcata aaccaaatga cgagcaaaag acgcatgctc ttccttgtat aaattatcaa
     4861 tccctaggaa attctcagca gacttggccc ccttagtagt gagccaaacc tcctctttct
     4921 cctctttaaa gatataatcc tctccttcga ccaaggttgt tacaagtgta tcaatgatcg
     4981 catagtaatt agactgaaca cgaggagaac ccgcaataat cagaggagtt tgtgcactat
     5041 caagcaagat atcatcaatt tcatcaataa taacatagtt aaacggtcgt aaaaacttac
     5101 cttcttcatt cgaggctagg ttatcattta gataatcaaa acctaaacta ctattggttg
     5161 tgtagatgat atccgaagca tagataagct tcttttcttc agctttcatt tccttctttg
     5221 gatcttccgt aaatggtaca ccaatggtca atcctagaaa acgataaact tggcccattt
     5281 cctcggcatc acgctttgat aaatactcat taggagtcac aaccatcact ccttctcctg
     5341 aaaaagcgtt caaatagaca ggcatggtag ctgtcaaggt cttaccttcc cccgtattca
     5401 tctcagcaac atttccatag tgcatgacaa tagctcccat gacttgaaca tcatagggga
     5461 acatccctaa aatccgctta tctgcttcac gcaccacagc aaaagcttca accaaaatat
     5521 cgtctagact ttctccctcg gaaagacgct gacgaaactc tactgtttta gctactaatt
     5581 cttgatccga aagagaggac atcttgcctt tcagggcatt aatctgcttt aaaatctttt
     5641 tcactttcct aagctggaaa tcttgaccta aacgtctaaa cacttctgat ctccttgata
     5701 gaaaaatgat aaaaagttaa ggactcaaat ccagcactta aaagagaaac ttgataagta
     5761 taactatctt ctggataagt aaatatgaaa tctttcgctt tttctacctt tttttctaaa
     5821 atatcaccat agcggtcaaa aaaagttatc ttggtataaa ctccattttc aggctgacaa
     5881 tcaaaaacca gtgatagttg atactcctgc tttcttttta ataagggaag ctgaggttgg
     5941 gttcgtgcag cctggtaatt gacactagaa aaccaagtct ttagaatctc accagatggc
     6001 aatagaggat tatacagacg aacacttttg tcaaggtaat aagatacagt tgttccatac
     6061 atataagtcc caccaacttc tccccaataa atagactgtc tctgtgtaat aatcatcttt
     6121 tctcccttcc aaaaccagtc tctaagacca ttctataaaa gtgcaaaaac caagaaatat
     6181 tggtatctgt atcatcattg tgtcgtccag cagttccttt agataaaatc ttcgcacctg
     6241 tattacacag ataagacact aattgttcat aggcttgtgg atccatttct tcatctttca
     6301 tataggataa accaaaggtt gtctttgaaa aatttgcttg tttaaaaatt ttccaaaaac
     6361 gctgatccaa ctccgtcata tcttgagaac ttgtcccacc tgtatgatga attaaacaat
     6421 caaaagctaa gttagagaca cccggtgcgt ccaaacgtcc acgactagct atagttccta
     6481 aattagccaa gggcttacca actatgatgg catggggttc aaaagaagca ccataataga
     6541 gagccggaaa cgttcccata gaaagacctg ataaaatcaa atccttatga tcaaagccta
     6601 aataatcaag atagtgagtg atcgtatcct ttacttttcc ctctagctca tccgttccga
     6661 gataaaaagc tcccccctct aaacgtggat cagaaaataa aatgaaggga catccaagag
     6721 ttttcatcat aaagtaaccc tcgaatcctt ctgcaggtcg ataacctgca aaatagacag
     6781 tcaaaggcgg tttaaaatca cctggatgga agaaatagtt tatttcatca cgcttggaat
     6841 catgtaggat atttccacca agtacaaact taccaaattg ttttcgactc catcgttggt
     6901 gaagattacc tagtttgata gttccacgac ctcttgcttc aacagatatc gagagaaagt
     6961 agtctccgtc tcgactatcc ataaaaagtg cttgctccaa gtctttttca gatactatta
     7021 cttcttcaaa gaaatcatca acagacccac tccacatttt acggattact aaacgaaagt
     7081 cacaattgcc ttccttctca tattcaagcc acaactcaat agggagtgtt ttttgcacca
     7141 taatattata agcccaatag gcaagctggg caaaatcttg cccaaactca ccttccaaac
     7201 tgacataatc caatccttga taagaaatag ctcctgtaaa atttggattg acttgtatcg
     7261 tcggaggaaa gagtttatca ccatacccac cgccaaaaag agatttactt aaatcattga
     7321 ttagttgttg gggacttaaa aaatcaattg cttgtgcaca tcgttttttt agaaaatcca
     7381 caacctctct atcatttatt tgtagattct ggttatagaa caaagtataa ggttcaaaat
     7441 attttacata ggaaaataag tctctggaat actgaccatc ttctattaaa actgcatgaa
     7501 aacggttaat atcctctttt tcgattattt cacgaagggt ttttgatgag tttgggtaaa
     7561 aataatacca actcatattt tcaggtatgt catagtgatt ttcccaattg gcaagtccta
     7621 tctgtaaaat attaagttcc tttgacattt ttaacctcct ctatccattt ctctaatttt
     7681 ccaagaaatt gttgtcctgt gtgctcctta atcttatcaa ttgaatatat caaggactca
     7741 ttccactcct tcaacctatc tgtgtaataa tgtgcagcct tagaaaattc tgtcacatct
     7801 gtcagcaaat aaccattttt taaatgttcc acatagacgg tttcaactag gttgatttga
     7861 ggaattcctg cactaatccc agcaatctgt gtgtagagat gaggatgtct atttaaatcc
     7921 acaatcaaac ggacaaattc tagtgttttt atcaaatcta actcatcatt caagttaaca
     7981 aactgtaagc gtaagtcctg gtgttgattt tcttccaggg gattttctgc atcaccataa
     8041 tcaatcgcct ttcccagatt ttcggattga atgttttctt ggatgaaaga ctcaacaatc
     8101 ccttcaacct cattcatttg ctcctgacta gcagcaaagg ctccaaaaat cacctcagta
     8161 tccttatttt cagcgacaaa ggacaagact tgaagcagag cttgattatc aatcccctgc
     8221 tcaaaatcca gttgaaaata aatgatggat tctttcttag tttggcttcg tcccaatcgt
     8281 aatcgggtgt caaaagatga taaatgataa cattgatgca ttcgttcagg atacaactcc
     8341 tgcaacaatc gtaaactatc ctctctatcc accaaaatca aatccgattt ttcaaaagtt
     8401 acatctaaat ccctaaaggt atcttgagga ttacgtccaa tgaacagact caacagttta
     8461 ggattagtac taggtaaacg atctagtacc aactggtcat gatgagaatg agaaggaagc
     8521 ataaaaatat cctgatcctt cacatacgtt tgcagataat tctcaaaaaa ttctgccacc
     8581 agatctccca tattttgata ggtaagcatt ttaaagcgat aacccaaaat tggattgact
     8641 tctacctgtc ctccttcttt taaacgctct ctaaattgcc aaactccctt aggattcaaa
     8701 tattcttgat aggctgcttg cccatcttca aaaaaaataa tactcgatat aaaccctcga
     8761 tcatccatga tatggtgcat atgtatttga ccatttggtt caaaatactg tatatcactg
     8821 atgaagcctt caactccatg ttcaacctta gcaaatttct tcccattttt ttgaacgata
     8881 atcgtaaagg gactataaat aaattcacag tcatcatccc actcaatatc tctaatgctt
     8941 aaaacttggg tatggagatt atgaaaatct tgcataacat caaaaacgga ataagtatcc
     9001 gtttctaaca caccatgtct atataaaaaa taacgtagat gaggctggta agctaatact
     9061 aatagacgag aatctatatc ttgctcttga aagagccgga tctggtgaaa ggtatcatca
     9121 aactctagac gaaaatgaga aaaataccat ggagtgatat ctgcatgcca tgttctttct
     9181 gacccatacc aagctggaat aaaataatac ataaaagtct cctaaatcaa aggtttatag
     9241 gtctcattta gccttaaagc cttgacctcg tctctaatcg taaaaatcat gcctccaaac
     9301 atcataaaca aacctggaat cattgccaat cgtaataact tttcatcaaa caaaacaaaa
     9361 agcatgggac cacctgccat aaccacatta aagagaccac ctatgactga gaaacgaagg
     9421 accaatcgat taataaatcg actagtatcc gcacctggat aaataccata aatgtattct
     9481 ccagattttt tcatacggtc tgcaatctct tctccattca tcgtaacaaa agcaaaaatg
     9541 atactaaata aaaataagac cgaaatataa acatagaccc acaaaggctt tccaatcata
     9601 aattcctttg ataaagccgc taaccctgaa tgattaggga aaataaatcc caacaagatg
     9661 aacaaataag ctggtacact aagaaaactc atcacataca tataaggcat cccacctgca
     9721 ggattcaaca tgatttcgag ataaaaatag cgtttaaatc gattgtgtaa gccaatttta
     9781 ttaacaggaa ccaaatagcg agctcgatac ataagggcaa gtaaataaga aaagacaagg
     9841 gttaataaag caagtaacac aataatccct gttggaatgt gtactgtctg aaatgtttcc
     9901 aaaacatcct gaggaatatt taaaaccata ctggatagga gaatgactat agaacctcca
     9961 atccccatac tcgcatttaa atctgacaac caaacaagaa aaaatgttcc tgctatcagc
    10021 aatattgtat tcattagaac aaccaatatt gcagaatagg aggattgtac tggcagtctc
    10081 aagctaactg ccaatgattg aatgacagct atcatcaagg tcaggtacat tttacggcga
    10141 tcttgtattt ctatagacgt agatgttaaa cccaaccgtt tagaaaaaga aaacatctgc
    10201 cataaaatca tagctgacat ccaaggggac aatccaacag aaaaaattga caaacttctt
    10261 agatttccac ctgttagggc ggctgagaag gctagatagg ctgttgaacc tcctaaaaaa
    10321 tctttagtat ttaggtcaac aaaagggaga gtaatacgac ttcccaatac atagataaac
    10381 agtagaaata aggtaaatag tccttttttt actgctattg acgaataaat cttagtcata
    10441 aaacactccc ctcaccttca ttgtttgtga tatggtaaat aacaattctt ttatttcaac
    10501 ttttaccatt tctttgttct gcatcaaaaa acaactttaa tctgaaacaa atatctttat
    10561 attcctcagt atctgttaat tgatggtatt ctaaagtttg cttataatct tttaaaagat
    10621 tgacaaatcg aatacgcaga acggagacat ccaaaccagc caagacacaa tccgatattt
    10681 tcttagaaaa cacttcaaca atatctcttg cccaggaaag atcaaattcc gttctcatga
    10741 tgcttcctga acgtcttcta tacagataga gattatcgtt tataaagacg attttagagg
    10801 ctaaaagata aaagcgatgc atagttgctt catcatcaaa gcggcgacca tttgaaaagt
    10861 gaacttcatt gaataattct tttttaatca acttaaatgt cggcaatata aaggccgagc
    10921 tattgaattt ccaatctcct gcttgacggt tcataatttc ttgtatagct aattcttcga
    10981 cgcaaaaatc ttgccctgtt acataaaaat aaaaattccc gtcactttcg tcataattat
    11041 agtaattccc tataacaaca tctgcatcct tttcttggac ttgctgataa agagtttcaa
    11101 tcatattttc ttttacgata tcatctgaat caacaatcgt gatgtactct cccttagcta
    11161 cactaatccc catattttta gcttgtgctg ccccccctta ttttcttgat gcaatactcg
    11221 cacacgatta tcttgcatca taaaagcatc acaaatttcc ccagaattat ccgtagaacc
    11281 gtcatcaact aaaataattt ctatattttg ataggtttgc ttcagaatat gctcgacaca
    11341 ctccactaaa tattttcccg tattgtatat cggtaccaca acactaatta attcactcat
    11401 tactctatct ctatattctt cattctatcc actaaatcat ctggtctctc cacagaaaaa
    11461 ataccgtcat agagtccatc atccatactt ttacgtgtga tatcaaaagc gaagattttc
    11521 ttgccacttt cttgtgccct tgtaactaca ttaaaaacct ctccacctgt attaatatct
    11581 aaataaacta tagaatcgtc caatagccaa tcaattctac tatgtaatac attttgatat
    11641 actgttacat tcgtatactg tgatagagag gtcagctcct cagaacaatc acacggtgca
    11701 gccaaatgaa aatgccaatc aggtaaccgt tgtactaaat actctaaatg ttttatatct
    11761 gcactccatg tcacaagcat cacttgtttt ttagactgat tgcttctttc aaaataattt
    11821 aaatcggaac gttgaaaagc aatctctgac caatccaaat ctctataaac ccaccataat
    11881 tcacgtagtc tagatatact atatgtattc caaggtttat catgactagc atagtgaaca
    11941 attgtaggtg gattgtcatc tagacgttca cattcttgag caaggtgata aatatcaaca
    12001 cccaccatat aattatatgt tttatctaag gctagccaat tatcctcaaa ataaatattt
    12061 aaaatactct gatccccaag atgaacaact tgattttgct cggccgctaa ttccaataaa
    12121 ctattgacaa tagagtgttc tttccatttt gcaacatcca ttagtaacat accagcatta
    12181 aatccagatt ttcgtccttc ataggcatag acatcatcaa cagcaccaat tgaatatcct
    12241 ttgagatcta tctcaaacaa agtagctagt tccccagtta caatgatatc ggaatccaga
    12301 tacaataccc tatcagattc aaccacttct gtcgcaaaaa atctaaagta agaagcataa
    12361 tttatatgag gtcctgtttt atagctttca aaaagttctt tatcaatgtg tacactgcga
    12421 attgtagaat tcacaacttc catttttctc cccaataatt taaaccattc aggagctata
    12481 tcactgttga gaatataaaa atcaacatct ctattgtgat acaatacaga ctttatagtc
    12541 gtctctaaag gaataagata ggcattatct gccgctaata ctattgattt tctcatactg
    12601 ttctctacct catgtaagat ctgattgctg caaccatttt tttcggaatg gagcttggat
    12661 agataccttc atatgaaatc tcagaaagat tttcagagca agtattgtcg aaagctatca
    12721 ttggtttttt gtacttaagc acaaactcat atgcatcttc taattttcga tcatgattca
    12781 aatccaaata caaatctgaa gctattacta tttgttccaa taacattggt aaggaatttg
    12841 gatatacggt aacattagga aaacgcgtta aagctagtag ttcatttgcc atatttgtat
    12901 aagcaacaat atgaaaatga acttctggca attgctccgc caaataatcg attttctcga
    12961 cacaccaaga attcgttaaa ttaacacatt gtaacttaaa tatttgtgat ttactaggat
    13021 acttcacact ctttgaaaac cattcattta aaataacaga ccaatccatc aaagagtatt
    13081 cccaccaaac ttctcttaga cgtccaacag aaaattgatt ccaaggctta tcctgagaaa
    13141 tatagtgtaa aattagtggc agtggttcga gcggaatatc aaaaatgaat tgatgtttaa
    13201 aggctgccgc cccataatca tatcctattt gaaaattata ttgatcttca agggaactat
    13261 attgatcttt aaacaacata ttcaaaattg actggtctcc ttcttccaca ttctcatgtt
    13321 ctttttctgt taagtcaatc aatttttgtc gaatagtttc agatccccat tttttgttgt
    13381 taatcaagag aacaccagca ttgaagccga ctcctgctcc aaagcaagaa cgagctgctg
    13441 ccaaataatt ttcacctaag tctaattcaa acaaatcggt caaatcacca gtcacaatca
    13501 aatcactatc tagatagaga actttatctt ctgttacaaa atctggaata aaatagcgtg
    13561 caaatgtcat atgattgata tgaggtaatt tattagacca attcatttga aactgtgagc
    13621 caattaactt gcagtcaatc aagtcgcccc ccatctcttg taaatatatt cttatttgac
    13681 taaaccattc ctgaggaata tcctgattta gcagataaat tttcaaatga ctattgtgtc
    13741 tacagagtga cttcatcgcc gtttcgattt gtcgaatata agcgtaatca cctgcaaatg
    13801 ctacagcgcg tttggtattt ctcattttaa atccttatca tttattttaa atagtctagt
    13861 ttaatattcg tgcaacaatc tgactgttat aggtttcatc atcaataaat tcaatatctt
    13921 gatcaaaatt atactctgca gtatgtttat gagaaaattt aaccttagtt ttagctccca
    13981 ttttcatcca ttcatactct gagtcaatat ggcctacatc caaaacttga tagcccatct
    14041 ggcatagatt ataactcaga acttttgctg taggacccag catacataaa atcaagcgac
    14101 caccagcata cttttcaatt tcttgttcaa tttcatgaac tctagaaaag gcactatgag
    14161 aaggacagat aattcgctta atagaatttg cctcatcgaa taaatcattt ccgacacctg
    14221 aacgagaagt cgcaccttcg actatcagta agtcacggtt ttcccaaatg cttttcaatt
    14281 tttcaaattg agctttagct tgactcttgt cttcaaaatc gatataaggg cgagatacaa
    14341 aggttgagcc ataccatgaa tcactgcata actctctgta aaaatccata taatgatcca
    14401 tgtgatcttt ccaaaatgga atcgcccacg atgtaaacct aaaacgatct gtaaaagcat
    14461 caggaaggca cactactaaa tcttctcgac tttcttggcc gataatgtcc ctcatgattg
    14521 aaaccaactc ttcatcataa tcctggtagg gaattgaatg ccctgcaagc atattgattt
    14581 ccccatctcc aaaacgaact aaagaagagt tgtgttcaat aatataatcc aaggtttcat
    14641 ctatcccttt cacctggatt atatcaagcg gtttttctag catatcctta gttatcaatt
    14701 tttgcaaatc agccaacatt cgttcaggat gttcacttga atacaacctt tggccattat
    14761 tcccattttg agatttataa aaggctagta taggtttaga tagatgagcc atttctgcaa
    14821 cgatatcgga tgttgaagtc agtaggttga tatctaaata agcatccatc ttctccttca
    14881 actcgtctag cttatctcta gaaattgccg gatagagctg aatattagga taacgattag
    14941 acaaatcata taattttggc cccatagctg tccaagcagc aacttgaaaa acaacatttg
    15001 gcaactgtcc agccaagtaa tccaattgtt cgatatcctg agaagccgta aacgttaaac
    15061 aagttaaact tggaagttgt cgaaaacagg taagatgtcc gtttttctta aaatctttaa
    15121 tcgtatcaat cataaaggaa actttcccat tttcgaaaac gatttgtcct aactctccgt
    15181 gctgagtcga ttgaaaagct agtagcgttt tttcttgttc ttgcaaagat tttaaaaagt
    15241 tctcatctgc actaccatga ttgatatcca aatatagatt tgttgacttt ttcagcttat
    15301 ctaatacagg aggaacaatt tgtggatgca atctcacatt atcatacaca gctaatttta
    15361 tcagcttatc acccatatcc gtccaagcag caatatgaaa taccacatct ggtaaagctg
    15421 tgactaactc ttctattcct tctaaatctt gggaatttgt caaagttaaa caagaaaatt
    15481 ccttatctaa agacgatgtc agttcaaact cccctatatg atgttgtaaa attttagtcc
    15541 attccaaatc atgaaattcc caccataaat cacgataacg attggctatc aaggtggtcc
    15601 agggttttct gtaagacaca aaatgaataa tactaggagg tactaactca ttaaaataat
    15661 tttcccattt attataaaaa gcagttacat catgacccac ttgaaggtta aattctttat
    15721 ccagctctaa ccaatcatct tgtaagacct gattaaaaat cgtttgatca ccattaaaat
    15781 gctcgaaacg gccttcttca acttccttca ttgtaacaat gctctgttca attagtcgtt
    15841 cttttaattt ctcttgacgc cattttttat tgttgattaa taaaacacct gcattaaatg
    15901 taattccatc tgtatcttta actgctgcga gtgatttttc ttctaaacaa atactaaata
    15961 atttctctaa agaagtattt acaatcaaat cactgtctaa atataaaacc ttatcttctt
    16021 ggatataatc tgcaataaaa tatctagtat aggtaatgct actaatgtga tcttgctttt
    16081 cccaatcttg aaacacagtt tgttcaggta gcttcacatc gataatctca ctacctaaca
    16141 tgcgagctat ttttcgtggt ttgcgaaacc aatctggcat aatatcttga tttaaaatat
    16201 aaatcttaac atctcgattg tggtataaaa tagattttat ggttgtttct aactgcctga
    16261 tgtaattacg atcaccagct aatacaattg ttttaatcat cttgttctct tattcaactc
    16321 ccaaatggta aattgcatct accaataatt ttttagtaaa ataaccttct tttagcaaat
    16381 aactaaaggt ttttatccga ttcgtcattt cttgatattc ttgtagattc attttatcaa
    16441 ctatcgcatg aacttcttct agactattcg ccacaaagcc taagccttga tctactataa
    16501 atttagcagt tgacaagcta cttggtacaa tgactgggat gcccgctgtc agataggtac
    16561 tcaccttatg agatatattt aaggtataat attggttgct ttctccatcg ttttgataag
    16621 ttccccagac aaggccaaat ccaccctttg ataattctag caacaattcc tcatcttttt
    16681 tccatccttc gatgctgaga tttctagcac tagaactagc ttccccttta tttgaaaata
    16741 ctctcaaagg cgtatcttga gaccaatttt gtaagtctgg aaaacgctct aaacttccag
    16801 caaaaaaaag ttctttttta aaagcagggg tgtataagga taaatcatga ggatgatccc
    16861 acatcccttg aacaagaatc ttcttagtcg tcaatccttc ttccatcagg cgtgttttca
    16921 ttctctctga cggcactatc aaaacatctg atagattata catgtacata tagtctttca
    16981 tgagataata gttactatca aacatgaggg gaacaacatc atggataaag caaataattt
    17041 tcacctgcat atccttcaac ttatcaaaca agagacgatc aaattcaaag ccattccagg
    17101 ttggagactg aaagactaaa atatccccaa tagaaatact ggccataata ccatccagac
    17161 gcttattcat ttcagaagga ctatctgaag caatgttgta aaaataaata ccaacctctc
    17221 taaatcccag ttgactagct atcttttgaa cagcattttg agctaagata accgtactat
    17281 caccagccat gccgtataaa tttgttaaat gtagtttcat attacgctat ctcatttttt
    17341 attactatct acggaagtaa agacaatttt tccatcattc ttctataaat ctctgtgaac
    17401 tgcatatttt gttcttctaa ttttgctatc actctttgta atctattttt ataaatcaaa
    17461 atatgattgg tcaagtcata gtctgaactt gctaaaatag ctatcttttc ttccctagcc
    17521 ccgatttcat catacatacg cttttcactc catgtattcg ataaagtatc agataaacca
    17581 acacggtacc agtaaacaca acgattcaaa tagactatcc tcgaagctct tagaagtacc
    17641 ttccatgtcc agtaagtatc ctctgcaatt tttcctatag gaaatggtaa atcttgtagt
    17701 aactctctct tgaataactt caagacagct acagtccagt tcccatttct gacttcttcg
    17761 acaccttccc tatccatgat tgctttacct tctatcactt ccagagaatc atctggatcc
    17821 gtaacataag tcatatacac atagcgtgtt tcatcataag aattataacg cccgatacta
    17881 atatctgcgt tttccttttt caaagcacca tataatcggt ctaaagcatc atgttccaac
    17941 caatcatcag agtctacaaa agtaatgtac gccccccccg aacattcaat acctaggtta
    18001 cgagctgatg aaagaccgcc gtttgctttc tcaaaatatt tgaaacgaga atctttctct
    18061 acaaattctt cacatatttt ggatgaatga tctggagagc catcattaac catgatacat
    18121 tcaaaattcg gataagtttg tttttgaata ctatccaaac actgtcgcaa ataattctca
    18181 acgttgtaga ttgggactac aatactaatt agttcatttt ccatatacta actttcctag
    18241 acttgattta ttctatttcc ctcttaatct cttctcttta caacaagatt tccattatta
    18301 taaactacac tgtttgatgg aatatcattg cgtatgagac aacctgctcc aataatggta
    18361 ttatccccaa tcgtcactcc ttttaaaatc gtgacattac ttcctatcca gcagtctctg
    18421 ccaactctaa tcggcgctgt agtccactgc cacttttcaa tcttttcagc agtataaata
    18481 tggtcgtgat cataaaaacg aacaccctct cccatcatgg taccagctcc gatttcaatt
    18541 ctttccatac agttaaaact acaagagttg ttgataaaaa caccatcatg cagtattaac
    18601 ttaccagaag agacatgaaa attttcaaaa tttctgcaga tgacatttgc tcctacaatg
    18661 aagtgagcct tatcatcgat tttaaagaca ttccaagtcc catcttggat gattttttct
    18721 tgatgattgg tgcctactct gacttcattc agatagtctc tcactctaga aaccaatttg
    18781 tctggtgtac ttgatagaaa aacttcctgc ccctgatttc catggactgt attgtcaaaa
    18841 gcaaagacag attttccagc ctgtacaaac ttggagacaa tagcatctac ttcttgaaag
    18901 tgattgatat ctagtaaaaa gtcacaggta tcgactagaa agtttagctg ttcttcattt
    18961 gtaatcgttc ggagacggac attatagttc gtttctaagc tacggatttt ctcggaaaat
    19021 tgaacaggtg ctgcaatata aaaacagata tttggcaagg attcaaggag ataatccaac
    19081 tcttcaatct gatcactaac agtcaaaatc agagcatctc ctactaaatg atttttcttc
    19141 atttgcttat ttctctcaat ttctcaatca ttgcttgaac ttggtcaaca gcatatacct
    19201 cctgacctgc ttcataggtt ttggtatttt caaaggacaa aatagttttt ccaagtcttg
    19261 caaattgatt gataatttcc tctgtttttt caccatgatt aatatctaaa agtacttgac
    19321 tggtttctac caattcatta tcgacatcta ccaaatagtg aattccgtta aatatagtca
    19381 cgtttggata aattgtcatc tgagccaatc gatcactaac tattactcta gctgctatct
    19441 taaactgaat atcaggcaag gattgaacca atgtctcaat ttgttcaata tggtctgagg
    19501 cagtatagat tagacaagtg aaaggttcct ttattggata gatgtgagat ctttgtaatg
    19561 gatgtaaatg atggttttgt cccaattctg tccattcaag cccatgatag taccaccaaa
    19621 cttcacgata ggtttgggcc gccaaatctt tccacggttt ccgatgagaa agatagtgaa
    19681 taatagcagg ataatcctga ccctcaggca attgataatc agcaaactgt ttatgaatga
    19741 caatatgatt ataatcaaag tccaattcca accatttatg ttcaaaaagc atattcaaga
    19801 tgctctgatc tgcctgatcc accttatcat gccattcatt ggttacatca attaattttt
    19861 gggtcatatt ctcttttttc caaaaagcat tgtttaccaa gagaacaccg gcattaaaga
    19921 tttcttgacc aaaataagct ctgcccccaa aatctctaac agcagccaaa ggataatctt
    19981 gtaagtctgt agcaaacaaa tcatcaagat ttttcgttac aagtaaatca cagtccaagt
    20041 agagggcctt atcttcttgc acgaaatcag ctatgaaata gcgtaaaaaa actgtgtaac
    20101 taatatccgt tttataacat gaaatttgct cagaagttac ccgacaatta ataatttctg
    20161 agtcaaactt ctctaagcgc ttatttaatt gcttaatcca ttcatttgga aaatcgctat
    20221 gaatcagata aaaacgaatc gaacgattat gataacaaat agacttgatt gtcgttaaaa
    20281 cttggtctac atagccatag tttgctgcta ggacaatggc tttcttttca cttgattctt
    20341 gtaccgttaa ctgatttaaa agtctttttt tcatttcaaa ctctttatat attgctgtgt
    20401 cagataaacc actagcttga ccgttggcga gactgacttc caacatctga cgataaactg
    20461 ccaagtgttt ctctagagga tatcccatat tagctagtag cgtaatacgt tcagacatag
    20521 catcaactaa ggcgtgcatc cacttttctg tccaaactct tgatagacta ccttttctaa
    20581 tccgataagc ataaagacct ttatttaaat aaattacctt ttccgataat aaatatacct
    20641 tttgattgag gtaaccatct tctcctaatt tacccatgtc aaagcgcaac tgctcaaaca
    20701 atcttgcctt atagagttta ccacaagcag atatcaaagc aaaactcttc atttcttgag
    20761 tttcataaca agttctcaaa gatggaaaca ttgtcatata ccttatcata ataggagtct
    20821 cctgatatat gaaagtagaa cattccttca ctttcgttga aagaatagta attcccaact
    20881 gcaatatcag cttgatattc tgttattttt ttatataggg tttctacata atctaactca
    20941 atccaatcat ctgaatccac aaaggtcaca taatttccgg acatattatt cagaccggtg
    21001 tttcgtgcgg cagaaatacc agcattttct tgttctatat agagaattcg gtgatccatt
    21061 tctgaaaatt ctttacaaat ttcacctgaa gcatccgtag aaccatcatt aacgacaaca
    21121 atctcaatat ttttatatgt ttgagaaata atactatcta gacatttcct cagatagttt
    21181 tctacattgt atactggtac aatgaccgta attttatcat ccactagcaa ttttctcctt
    21241 gatttatgtg cgcgagttag tttagcctaa ctttacaggt tgtcttactc ttcttcatca
    21301 cgtttacggc gtttcgcaac caatccaata cctgtaacag ctgctagaac tccaagtaac
    21361 acagatccaa ttgacgactc agtacctgta ttaggcaatt ctttttggga tttacctgtc
    21421 gatccagaag catttccaac ttgtgagttc gaatggtttg ctgaatttga aactgatgta
    21481 cttgctgagg ctgacgcact cgttgatgct gactcagacg cactggttga tgccgattca
    21541 gacgctgagg tgcttgctga tgctgaggca ctggttgacg ctgaagctga ggcgctagta
    21601 cttgctgaag ccgaggcact ggttgatgct gaggctgagg cgctagtact tgctgaagct
    21661 gaggcacttg tcgatgctga ggccgaagca ctggtacttg ctgaagccga agcactcgtt
    21721 gatgctgagg ctgaagcact ggttgacgct gaagctgagg cactcgttga ggctgaagcc
    21781 gaagcactcg ttgatgcgga agctgacgca ctggtacttg ctgaagctga ggcgcttgtg
    21841 cttgctgagg ccgaggcact tgttgaggcc gattcagaag ctgaggtact cgttgatgcg
    21901 gattcagatg ctgaggtgct tgctgaggcc gaggcgctgg ttgatgcaga agctgaggca
    21961 ctggttgacg ctgaagctga ggcactggtt gaggccgatt cagaagctga ggtactggtg
    22021 cttgctgaag ctgaggcact cgttgatgcc gattcagacg ctgaggtgct tgctgaagct
    22081 gatgcacttg ttgatgctga ggctgaggcg ctggttgatg ccgattcaga cgctgagata
    22141 cttgctgagg ctgaggcgct tgtacttgct gaagtcgacg cactggttga ggccgattca
    22201 gatgctgacg tacttgcact tgttgacgcc gatgtgcttg cacttgtcga tgctgacgtg
    22261 cttgccgatg tacttgtgct tgttgatgct gacgtgcttg ctgaagccga agcactggtg
    22321 cttgctgaag ccgatgcact tgtacttgct gaggttgatg ccgattcaga cgcactcgtt
    22381 gatgcggaag ctgacgcact ggttga
//
LOCUS       DAVBHS010000015        21811 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVBHS010000015 DAVBHS010000000
VERSION     DAVBHS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21811
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.015"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..349)
                     /locus_tag="VQ651_000415"
     CDS             complement(<1..349)
                     /locus_tag="VQ651_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HET2753457.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIF"
     gene            complement(336..1106)
                     /locus_tag="VQ651_000416"
     CDS             complement(336..1106)
                     /locus_tag="VQ651_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET2753458.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            complement(1132..1986)
                     /locus_tag="VQ651_000417"
     CDS             complement(1132..1986)
                     /locus_tag="VQ651_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HET2753459.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELMPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYLNNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            complement(2143..2547)
                     /locus_tag="VQ651_000418"
     CDS             complement(2143..2547)
                     /locus_tag="VQ651_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET2753460.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            complement(2617..4098)
                     /locus_tag="VQ651_000419"
     CDS             complement(2617..4098)
                     /locus_tag="VQ651_000419"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HET2753461.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIVYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSADHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEHVGILLD"
     gene            complement(4117..5295)
                     /locus_tag="VQ651_000420"
     CDS             complement(4117..5295)
                     /locus_tag="VQ651_000420"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET2753462.1"
                     /translation="MAQHLTTEALRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVFENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            5460..6467
                     /gene="galR"
                     /locus_tag="VQ651_000421"
     CDS             5460..6467
                     /gene="galR"
                     /locus_tag="VQ651_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HET2753463.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFRNYSQAKGIYHDELVFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHDRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(6513..7550)
                     /locus_tag="VQ651_000422"
     CDS             complement(6513..7550)
                     /locus_tag="VQ651_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET2753464.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAVEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLQSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(7551..7904)
                     /locus_tag="VQ651_000423"
     CDS             complement(7551..7904)
                     /locus_tag="VQ651_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET2753465.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(8148..9038)
                     /locus_tag="VQ651_000424"
     CDS             complement(8148..9038)
                     /locus_tag="VQ651_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET2753466.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            9171..9707
                     /locus_tag="VQ651_000425"
     CDS             9171..9707
                     /locus_tag="VQ651_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET2753467.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(9874..10680)
                     /locus_tag="VQ651_000426"
     CDS             complement(9874..10680)
                     /locus_tag="VQ651_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HET2753468.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(11025..12545)
                     /locus_tag="VQ651_000427"
     CDS             complement(11025..12545)
                     /locus_tag="VQ651_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HET2753469.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLAALIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLVQKEKENLVIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGKTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMEPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(12538..13266)
                     /locus_tag="VQ651_000428"
     CDS             complement(12538..13266)
                     /locus_tag="VQ651_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2753470.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            complement(13281..14045)
                     /locus_tag="VQ651_000429"
     CDS             complement(13281..14045)
                     /locus_tag="VQ651_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET2753471.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVVDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(14049..14492)
                     /locus_tag="VQ651_000430"
     CDS             complement(14049..14492)
                     /locus_tag="VQ651_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2753472.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(15174..15470)
                     /locus_tag="VQ651_000431"
     CDS             complement(15174..15470)
                     /locus_tag="VQ651_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET2753473.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETAKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(15492..16556)
                     /gene="pepA"
                     /locus_tag="VQ651_000432"
     CDS             complement(15492..16556)
                     /gene="pepA"
                     /locus_tag="VQ651_000432"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HET2753474.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            16957..17658
                     /gene="galE"
                     /locus_tag="VQ651_000433"
     CDS             16957..17658
                     /gene="galE"
                     /locus_tag="VQ651_000433"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2753475.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            18081..19040
                     /locus_tag="VQ651_000434"
     CDS             18081..19040
                     /locus_tag="VQ651_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2753476.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKEISESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLGFILLLWRGGRKDAD"
     gene            19030..19986
                     /locus_tag="VQ651_000435"
     CDS             19030..19986
                     /locus_tag="VQ651_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HET2753477.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLTLQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            19983..20735
                     /locus_tag="VQ651_000436"
     CDS             19983..20735
                     /locus_tag="VQ651_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2753478.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGS
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            20831..21796
                     /locus_tag="VQ651_000437"
     CDS             20831..21796
                     /locus_tag="VQ651_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2753479.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPAILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
BASE COUNT         6472 a         5083 c         3859 g         6397 t
ORIGIN      
        1 caaatatacc tgatttttcc aaaaacatag tatattcttc aatactatag tttcgtttac
       61 taaaatcaaa atagaaatct tttatcggat ccttaacaat aatcgtatct ctcaaacgtt
      121 tagcaataag tagaggcaca actttcaaaa tttctggata attttgatat aagtctaaaa
      181 aatcctcttg tatattttta ctaccaataa gactattcat aatatttaga gcaactttta
      241 tactagatac atttttataa accttgggaa aatctgtata ataagtccag tcagctactg
      301 tgtcggtcat cgttgacaac cactcatcaa aatttctagt ttgtttcatt ttctgcctcc
      361 aacctcttcc ttgcaatttt taaatattct ttttcagcat cgatacctat aaatcttcta
      421 cctaaccgct tcgcaacaac acccgtagtg ccactaccaa caaatgggtc tagaatatag
      481 tcaccctctt tagtagaggc taaaataatg cgttctaaca aatactctgg tttttgagtt
      541 ggatgtttcc cagcccattt ttcaactttc tttgttaaag aacccgtcca gacatctttc
      601 atttgttttc catcattcaa ttctttcatt aaatcataat tgtagtaatg acgagctttt
      661 ttatcatttt ttctggccca taaaatggtt tcagtagaat gggtaaaata acgacaagat
      721 aaattggggg cagggtttgt tttctgccaa gtaatattat tcagaatttt aaaaccttct
      781 tgttctaatg ccattccaac tgagtatatg ttgtgcaaac tacctgaaat ccataccgtc
      841 ccattaggct tcagaacttc ttttgctagg cgaatccatt tacgattaaa ctcatgtttt
      901 tcttcgaatg aagaaatttt atcccaatct cctttatcaa cagaaacaac ttgtccccca
      961 gaattagata ttccaccatt acttaaaaaa taaggtggat cagcaaaaat catatccata
     1021 ctttctggtt tcatttttga taagaacttg aacgtatctg aatgaacaag aatcatctta
     1081 tttttattgt agtatggttt tgtcataaga acacctccat acttatattc gttatttttc
     1141 ataatttgtg actataattt cagaaatttt tcctcgactt gaagattttg ctccattagt
     1201 tcgggtagct tcaacataat gtatattaaa atccttatac aactcctcta ctaaagcact
     1261 agaagaattt gataacataa cataagctcc tgtatcactc aatctcttaa aggcatctct
     1321 taatcttact tgatctgcaa aagagaatcc ctcatgagta taagacgtaa atgcactcgt
     1381 ctcagacaat ggaatatatg gagggtcaaa atacacaaaa tctcctgttc gaacatctac
     1441 aattgccttt tcaaaatctc ccactttaat ttctagttga ttgttattta gataaactga
     1501 aatagcagat atcaattcct catcaacaat tttaggattc ttataacgtc catatggaac
     1561 attaaattgg ttcttagaat tcacacgata tagaccatta aagttcactc tcaacatata
     1621 tagaatacgt gcagctcttt gtacttcgga catcatatct attctttcat cacgatctgc
     1681 agaacgtaaa tctaaataat attcttttga attatattcc tgatgaactt tcaaaatttc
     1741 aatcaattct tgaggattgt ccttaatttg ttgatagcaa tttattagtt cagcgttaaa
     1801 atcattaata actgcatctt taggagccaa atcaaaaaat aaagctccac ctccaacaaa
     1861 aggttcgaaa tacctgttat aggttttagg cattaattct ctaataacag gcaataattg
     1921 tcttttacca cctgtccatt tcgtgaacgg ttgtaaagta actttcttta tttcttttat
     1981 cttcatgctt ttaattatac catagaaatt aaaacacaac tcttgtattt tctaccacat
     2041 tactaaattt tctattttag aattaaatca tatcaatata aaaaaatgag taaggcaaat
     2101 cttataaatt ccaaaattca cctcactcta ttaaattact ctttatatcc ttacccgtct
     2161 ttcctcagac ctttggcctg tgacaaacat agtaaaggtt gctttgcaga catttctgcc
     2221 ctcttgattg gtgatatcca catccatcac acaggttgta cgaccttgat ggacacattc
     2281 tcctttaatg gtcaatacat cgtcgagttt tcctgcctta aggtagttga tagaggattg
     2341 gagtgtcacc ccatcaagtc ccagcgagat aaccaccaaa ccactgattt ggtcacatag
     2401 tgtgaataga tagccaccat gggcattgcc atagtagttg agcgacgagt tcactacttt
     2461 ggtcgtcacc acaacgtgac catctctcat tttttcaatt tcgtaatttt caaaggcaga
     2521 tatagcgtca aaatgaaagt ctttcatcgt ttcctcctga tatttcaaat gacactatga
     2581 tactactttt tataggactg tgcaaggaga aagctcctag tctagtaaaa ttccgacatg
     2641 ttccacaaag cgcataaagg ctgtctgccc ttgttctgtc tgcttgtaga ctcctgcatc
     2701 ctcaagtaca cgcgcaaaga tagcacccac agagtccttg acgattgcaa gggctttttc
     2761 tttatccgtt aggtctggat gttgggattt gagttggtct gcccactcct gatgataatc
     2821 ggcaactgta acagcttctc ctacaagata gctagcgact tgctctactt cttctttcag
     2881 acgtggtggc aagattgcca agcccatgac ctcaatcaag ccgatatttt ccttcttgat
     2941 atgttggaca tccttgtggg gatgatagat gccatcagga tgatctgctg aagtctgatt
     3001 gtctcgcaag accaagtcca actcaaactg tccatcacgc ttacgcgcta ttggtgtgat
     3061 ggtgtgatgc ggtgtcccgt ctgtctctgc taaaatctgc acactagaat ctgaatactg
     3121 acgccattcc tggaaaatct tatcagctaa gttaatcaaa tcctctttgg aatccgaagt
     3181 caagcgtagg acagacattg gccacttgat aattccagcc ttgacctgct caaaaccagc
     3241 aaatcggaag gtcttttgca agggagccaa ttccatagga aatacgtgac ggcctccctg
     3301 ataatgatca tgagttagaa tagagccccc cacaatcggc aggtcggcat tagatccagc
     3361 aaaatatcct ggaaactgct ctacgatagc caacagacgt tcaaaactct gacgactaat
     3421 ggccatggga cgatgctggc catctaaaaa gatacaatgc tcattaaagt aagcataggg
     3481 cgaatactgg aaaccccatt cctgaccaac catttcaaaa cggataatcc ggtggttgct
     3541 gcgagctggg tggttgaccc gaccatggta accctcattc tctaaacaaa gttgacactg
     3601 aggataatta ctagcttgca ccaacttggc taccgcaatc tctttcggat ccttttcagg
     3661 cttagagaga ttgatggtaa tttcaagttc tccataatca gatggaacac gataaacgat
     3721 atttttagca atggccttga gtttgatgta gtcatttttc tgactgagtt gataaaaatc
     3781 ctctatcgct tgctcaggag agtaggcata ggcttcccag aagtcacgat tgacctgact
     3841 cggacaagga gtcaccaagt ccatcaattc agtaccaagg atttcacgcg cagtctgact
     3901 atcctcaatc gtctctaatc gaacggcttc ctcaactagc tggtccttga ggtcaatcaa
     3961 tttatccaga ttggtctcaa cttccaaaac accttctccc actcgtgcca aaacacgatt
     4021 ggtcaggtag attcgatcca tttcctcaaa tgaactttca gaaatgacat gtgttacaaa
     4081 tttatctact aaggtcacta tagagcctcc ttttgactag tcaaggacgc gagtgccacc
     4141 tgcaacttca gcgatataga agcttggagc gtatccaact acttcctcgt agtgtttgcc
     4201 tacagcttcc ttaaaggcct caacagtatc tttttgaacc aaggcaatgg cacagccacc
     4261 aaaaccagcc cctgtcatac gagcaccgag aactccttct tgtgcccaag ctgtgtgaac
     4321 aagagtatcc aattccaaac cagttacttc ataatcatgc tccagagaaa cgtgtgacgc
     4381 attcatcaaa cgaccaaatg tttccaaatc acctgcttga agggctgctt gagctttaag
     4441 ggtacgttgg ttttcaaaca cagcatggcg agcacgtttc aaacgatttt catctttaat
     4501 cagatagcta tattggtcaa cggcccactc gtccaattca cccagggtct gaatatccaa
     4561 ggcaacttgc aattcttcca ctgctttttc acactcagca cgacgttcat tgtatttaga
     4621 gtccgccaat tcacggcgtt tgttggtgtt catgataaca acgacattgt ccttcaaatc
     4681 aagtggcacc aagtcgtatt ctaaagtatt ggtatctaag taaatagcac gttggtcagc
     4741 ccccatacca atagcaaatt ggtccatgat accagagttg actccgataa agttattttc
     4801 tgtttgtttt ccgattttaa ccaaatcgag acgctctagt tttaaatcaa agagatgctc
     4861 tgccacgact cctgtcaaga gctccaagga tgctgaagaa gacaaaccag caccatttgg
     4921 gatattccca taaacataaa aatcaaaacc tttgtcaatc acgtgcccag cttcttgcaa
     4981 gaaatgaagg acaccttttg gataattggt ccagttgtgc tctttttcaa acttgaggtc
     5041 agcgagaggc acttcgataa tgcccttgtc ctcaaagtta gctgagtaga aacgcaagac
     5101 ttggtcgtca cgcttacgag ctgcaccgta agttcccaag gaaatagcag caggaaaaac
     5161 gtgcccaccg ttgtagtctg tgtgttcacc aatcaaatta atgcggcctg gtgaaaagaa
     5221 ggtttggtct gcttcttgac caaaaacagc aagaaagtct ttgcgaaggg cttcagtagt
     5281 aagatgttgt gccatatgaa ttctcctttg actgtccgtt aagccacctt aacgtataat
     5341 cgttttcttt ctattgtatc caaggaattt tccaagatca atccttttta ctaaaatttt
     5401 agtaaaatat cagtaaaaag aagaaaaaca tgatatacta acctaatcaa ggaggattta
     5461 tggctacctt aaaagacatt gcacagctag cctctgtctc tatcgcgacc gtatcccgcg
     5521 tcctcaatcg cgaccagagc ctatctgtta cagaagaaac cagacaccgt attttaaccg
     5581 ttgctgaaga gctgggctac accaagcacc tcaagacagg cgagtcccac aaacccaagc
     5641 aaaagattgc cattatccaa tgggtcagcg aacaagggga gctggacgac ctctactact
     5701 accagattcg cctaggaata gaaaaaagag cccaagagtt ggactatgat atcttgcgct
     5761 attttaatga ccatcctttt accttaagcg aggaagtgat tgggattctc tgcattggaa
     5821 aatttagccg agctcagatt tctgcctttg aagaatacca aaaacccctg gtctttctag
     5881 acagtgatac actttctctg gggcatactt gtattattac ggatttttac actgccatga
     5941 aacaggttgt cgattatttc ctcagccaag gaatggaccg tatcgggatt ctaacaggcc
     6001 ttgaagaaac aactgaccaa gaagaaatca ttcaggataa gcggctggaa aatttcagaa
     6061 actacagtca agcaaaagga atctatcatg atgaactggt ctttcaagga aactttactg
     6121 cccagtctgg ctatgacttg atgaaggaag ccattcagaa cttgggagac caactcccac
     6181 cagcattttt cgcagccagc gatagtttag ctatcggtgc ccttcgtgcc ctccaagaag
     6241 ctggaatcag cctaccagac cgtgtcagcc tcatttcctt taacgacacc agcctgacca
     6301 agcaggttta tcctcccctc tccagcatca cagtttatac tgaagaaatg gggcgagcag
     6361 gtatggatat tcttaacaag gaagtcctcc acgatcgcaa aattcctagc ctgaccatgc
     6421 tgggaaccag actgacatta agagaaagta ccctaaatca agaataggat aacataaaaa
     6481 acgaatagag ttctaaaact cctattcgtt ttttattcga ttacaatcat agacttaatg
     6541 gtcttacgtt catccatatc tttgtaggct tggtcgatat cttccagttt ataacttgaa
     6601 gtaaagacgc gacctggatt gatatcacca tcaaggacgg cttttagtaa aaattgctta
     6661 tcgtatgttg tagcagaagc tgccccacct gctacagaga tattttgcat aaatgtcgaa
     6721 ccaagagcac gattattata gtgtgggact cctacaaagc ccatacgccc tccattatga
     6781 agaacaccta gcgcctgttc tatagcagcc tccgtaccaa cacattcaag tgctgcgtct
     6841 gctcctccac cgaggatttc acgcaccttg gtaattcctt cttgaccacg ttctgcaaca
     6901 acagctgtcg cacctgactc catagccatc ttttgacggt cttcatgacg gctcataagg
     6961 ataatttgtg atgctccacg catcttagcc gcgatgacag cacattgacc aacagcccca
     7021 tcaccgataa caacaacctt gtcccctttt tgaacatttg caacacgcgc cgcatgatag
     7081 cctgtcggca tgacatctgc aagagtcaaa agggactgga gcatcccttc tgtatagtca
     7141 gaaggttgac cagggatttt aaccagcgcc cagtttgcat agtggaagcg aatatattct
     7201 gcctgaaaat caccccccaa attattgcca atatgattgt cgcaagaacc gtcaaatcca
     7261 gcaagacagg catcacactc accacatcca tgtgtaaaag ggacaatcac aaaatcacct
     7321 ggtttcaccg tcgtaatggc ttccacagct tcttcaacaa tcccaatcgc ttcgtgtcca
     7381 ctatttttgt gtccagcttt cgtttctgga ttacggtacc tccataaatc tgaaccacaa
     7441 acgcacgcac gaaccacacg aataatcaca tcatccgctt ctattatttg cggacgttca
     7501 atgctagcaa gtccaacctg acctgccttt gtatatactg ctgatttcat ttaaaatttt
     7561 ccttccttat aaagtttaat tttgagattt aaacgattta aagctgtctg tagctgagac
     7621 aagcgctcct ctaatttttg cttttcctct tctaaaatac caagcctctc ctctctcgtt
     7681 tcatctccct tttggtagag cgacatatag tcaactaaac tatctacaga gacacccgcc
     7741 gaacgaaaac acttaataaa ttccagcgct tcgatatcct gatcttgaaa atcacgaatc
     7801 ccagtagcag tacgagtaat cggtggcaca agaccaaccc gttcataata ccgaatcgta
     7861 tccgctgaaa ttcccaacaa atcactggca gatttaatat tcacacgcta acctcctctc
     7921 atttcattca ctgaattcac gctattctaa ctcacttatc ttattatata actttgagtt
     7981 gactccaagt caagcatttt gtatctaatc gaaaaatttt tatcattaac aatcatatat
     8041 ctggagttta tacaatgctc ttctcttacc aatttttgaa atatttaaat aataaagaaa
     8101 ttactctatt ccaaataaga tagtacccaa gtatttttca cttttttcta atgttgatgc
     8161 tcataactcc gttccaagtc acacaccttt cgcttatggg tttggtgagt ttctaggtca
     8221 gcatcaattt caatggtaat attttgaaaa ccacaatctt ttaggaaaat tcgaatagac
     8281 tctttacaag tttccatatg ttccatttct tttagacaaa catggacaat ggcatttttt
     8341 tccaaagcat ccatagtcca gagattaagc tgattaaggc tggccacatt gtccaatcgc
     8401 tccaggccac tctttacttg cttgatatca agaccttctg gcacagcatc caagaaaatc
     8461 ttgagtgtag accaaaaacg tggaagggct tttgaaagaa taaagaaaga aatgacaagg
     8521 gacaaaagag gatctaggat ataccagtcc gtaaatcgaa gaacaatcgc catcaggata
     8581 acagctaccc accctagcgt atcttccaga aaatgcagac tcagaataga ctcattcttt
     8641 gtctttccct taccaaccac cagactcgct aacagattga tagtaatcgc aataattcct
     8701 aaccagagaa tcccctcatc attgactggt tgcggatgca aaatcttcgt gacattttcc
     8761 aaaatgacta gaacagagcc cgttacgaga atcacagctg ttaccaaggc tcctagcagg
     8821 ctaaaccgct tatagcccaa ggtgtactga ttgtcttctt cacgattgga gattgtttct
     8881 agaaaagctg atattccaat tgcaatcgca tctcccaagt catgcacaga gtcagcaaga
     8941 acagcgctag aaccaaatac tccacctgca ataaactcaa caatggcata agtcaaattt
     9001 aagaaaaaag ccacccaaac agcatatttt gccttcatat ttctcattcc tttgttataa
     9061 tagatttatg aacaccttgt tcattatcat tatccactaa aactcaaaga aaggatagaa
     9121 gcaaaacgtc agctttattc agttctgaac aatttgcctt aagtgtccat atgactaaca
     9181 ttgaccgccg tatcagcaaa accaaaaaag ccatctatca agcttttata caacttttga
     9241 atgctaaggg ctacgaggcc actactgttc aggatatcat tgatctcgca gatgtgggac
     9301 gatccacctt ttactgtcac tatgagagta aggagcttct tctggaccag ctctgccgct
     9361 acctcttcca tcatcttttt gaaagagagc aagccatttc aaccgaggac tacctcgccc
     9421 acctctttct ccattttcat aaaaaccaag accatatcac cagtctgcta ttttccaaaa
     9481 atgactactt cctccgtcaa ctccataaag agctagaaca ccatgtctat tccgtgctag
     9541 ctgataattt aaaagaagcc caccctaatc tgcctacttc ttacctccaa cacttggtca
     9601 tgtccaactt tatcgagaca ttgacctggt ggctcaaaaa aggtcaagat ttcacagacc
     9661 aggaagttgt ccaattttat ctagaccttc tcattcctaa aaattgaata tagagtaaag
     9721 cttcagttgt cttatttcta ggttactgag ttttttatct tttcaacaac aaaagaggac
     9781 ccgccgatcc tctttttcat actataaatc cttgattatc aactatatct gttttaatcg
     9841 aaatctcaaa acagcacttt caaacatctt ttcctagtta agtaaatcag tattctgctt
     9901 agctgccttg ctccattgat accaaccaac tagactgtta atgagataaa ttagatattt
     9961 cccttgaatt tgcaggcttt ctccccacca gagatagatt gaaaagacat tggtagccgc
    10021 ccagaatatc cactgttcac ggtaaacagc tgtcatgagg atttgcccta ccccattggt
    10081 tgcatctgtg attgaatcac gatagggacg attggcacca atagactgat aaatgaagcc
    10141 aaaggccaac caccaaagca cactaatgga aagatacttt gtccagccct tgccgtccag
    10201 tttacgcgcg acaaactcct gcttttcctt cttaaactgt gcctgataaa tccaaactag
    10261 aagtccaatt ggctgcatga ctgtgaagta aagtgtcgtc agcacctcac cataaaagcc
    10321 tttctgtagg gccaaaataa ggtaaataac agagttaatc aagccaaaaa gataattact
    10381 tgctcgacct tccgatacaa agattacaca gataatccct gtcaagctac aaatcatccc
    10441 aatccagtca acaatacgat gttcgtaaac caactccagc cagagaggaa aacttcctaa
    10501 aaccagcaaa taaatccact gggcaaaact acgatgggca aagaggtcat cccagatagc
    10561 cttcatagtt cctgaaaatc ctaaatcagc catagccgca accatacgac ggtaaccacc
    10621 tgacatttca cctagggttg ttttgatatt ttcaattttc ttttgcaaat aagtatgcat
    10681 catttctcct tttgttttta aagagccgtg tctggataga ctttcggacg caacgctcta
    10741 ttagataatg aactgcctat acacaagatt tctaacctta gtcgacatga gctgaaacct
    10801 cttatttgtt aagtagttca caaaatatta tacacctatt ttatgaatag tcaactgtct
    10861 ttacagtaaa attttagaaa atcatgaaaa ttttctcttt ctttccattt taagtgacat
    10921 tcagtcattc tcacatcaaa aaagcccaga cgaaattgtc tgagcattct tttatctagt
    10981 cgtttaagga agttgagttc agtatgttta aagtctctgt cccatcattt cttcaacaaa
    11041 ccttgttctt ggagaaactc cttggctact tgctttgctg acttgccttc aacaccgact
    11101 tggtagttga gctggctcat ctggctttct gtaatcttac cagccaatgt attaagaact
    11161 ctttccaact ctgggtgttt cttgagaaga gcttctttca tgagtggagc cccttgataa
    11221 ggtgggaaga gttgcttgtc atcttccaag acctgtaaat cataacgctc caattccgca
    11281 tcagtcgaat aggcatccgt gatttgaata tcccctgact gaatagcctg atagcgaagg
    11341 gctggctcca tggtcgctac attgagattg agaccataca ttgattgcaa gcccttattt
    11401 ccatcttcac ggtcgttaaa ctcgagtgta aaacctgcct tcaactgccc ttccactttt
    11461 ttcaagtctg aaatggtctt caagccatat tcttgagcaa tctttttcgg aacagctaca
    11521 gcataggtgt tttgataaga catgggtttg agataggcta gatgatcctg cttagcaatg
    11581 ccatcacgcg ccacctgata aacctgttct ggttcatgac tcaccttggg tgatggttga
    11641 agcaaacttt cagtcaccgt accagtaaat tcaggataga tgtcaatatc gccttttttc
    11701 agagcttcat aaaggaagct tgtcttccca aaattcggtt taacagtcgc agtcatgctg
    11761 gtattttctt caatcagcaa cttatacata ttggccaaaa tttctggttc tggacctatt
    11821 ttcccagcaa taaccaagtt ttccttctct ttttgaacca aaagagctgg actataagac
    11881 agacccagta ataaagccac caaggcaaaa cctgagaaaa tcgtccgtaa ttttgctttt
    11941 tccatcactt ttagtaggaa gttaaaggca atggctagca ctgcagaaga aagtgcccca
    12001 atcaaaatca aactggcatt attacggtca attcccaaaa gaataaagga acctagtccc
    12061 cctgcaccaa tcaaggccgc caaggttgcc gtaccgataa tcaaaacagc tgccgtccga
    12121 atcccagaca tgataacagg catggcgagt ggaatttcaa atttcttgag acgttcccat
    12181 ctggtcatcc caaaggcaat cccagcctct tgcaggttcg gatcaattcc cttcagccca
    12241 gtgatagtat tttgcaaaat agggaaaatc gcataaatca ctagagctgt caaagccggc
    12301 aaggtcccaa ttcccatcaa agggataaag agccccaaca aggccagaga cgggatggtc
    12361 tggaaaatac ctgcaatctg caagacccag tcggccagct tctcatgata gcgaagaaaa
    12421 acagccaagg gaatcgcaag caaaatagct agtaacaagg tcaaaagcga caactgcaaa
    12481 tgttgagata gagctgtcaa ccaatcacta aaacgatcct gaaaagttgc aattaaatta
    12541 gtcatgaaca ctacctccaa acaagtctgc tacaaagtct gttgcaggcg cttttaaaat
    12601 tgtctcggga ttcgctacct ggcgaatttc tccatcctgc aagacagcaa tacggtccgc
    12661 caacttcaag gcttcatccg tatcatgggt tacaaaaatc gttgtcatcc caaactcttt
    12721 atgcaattct tttgtcagaa cctgcaactg ttttctcgaa atagcatcca aggccgaaaa
    12781 gggttcatcc atgaggaaaa tcttgggctg accaatcata gctcggacaa taccgacccg
    12841 ttgctgttct ccaccagata attcactagg taagcgatgc ccatactcgg ctactggtaa
    12901 accaacctta gccaaaagct cttctgtttt cttcgtaatt tcttccttgc tccacccctt
    12961 catttcagga atgagagcaa tattttccgc aactgttaga tttggaaaaa gagcaatagc
    13021 ctgtaaaaca taaccagtag aaagacgaag ttcacgctca tcatagtctt tgatgcgctt
    13081 cccatccata taaatatttc catcagttgg ttccaaaaga cggttaatca tcttgagcat
    13141 ggtcgtctta cctgacccag aaggccctac taaaaccata aattccccat cctcaatctg
    13201 taagttgaca tctctcaaga catccttttc tgtgtagcgc agtgctacat ttttgtattc
    13261 aatcattctt tgtcctcaat ttaaaacttc cctcgattgg tcaagtcttc taccttaggc
    13321 ataacttcct tattatccca atgctccaca attttcccgt tctctaaacg gaagatatcg
    13381 tactgggcat aagcaacgcc atcaatctga gtctgaccat agctaaccac atagtttcct
    13441 tgtcctaaga gttggaaaac aaagtcaaaa gtgacactat attcagccac atagttttta
    13501 taagcagcac ttccttgtcc aatatcatga ttatgctgaa tcaaatcgtc tgccacataa
    13561 tcactccact gctctagctc cccattttgg aaaatttctg tcaagaaacg gcgaaccagc
    13621 tttttatttt ctgctttctt gtccaaatcc ttgatttcaa aatctccaaa aatttgatct
    13681 agttggtcat tttcaggtgt tcgatagtag tcaacgacat cccaatgctc aacaatacga
    13741 ccattctcat ccgcacggaa agtatccgtc gtcacccatt gagcttctcc accattcaga
    13801 tattgatgaa catgaacaaa gaccagattg ccatcctcaa tggtgcggac aatcttaatc
    13861 tgacgctctg gatgacgctc aaagaaatct gcaaagaagg ctgcaaatcc ttctttcccg
    13921 tcaggaacac ctgtcgaatg ttggatatag gtatccccta cagactgggc ttgagcctca
    13981 gcaactcgcc cgtcttgaat ggcatggatg tataggttgt gagcattttt cacttgttgt
    14041 gacatattct aaacctcatt tcccttctct ttcagattcg ccaaaattct ttcttgaaaa
    14101 ccttcaaatt ggtgaatttc ttcctctgaa aatcctttgt aaaagatagt atccaatttc
    14161 tgactgacac gatgccccac ttctttctgg aacttgccta actccgttaa aactaaatac
    14221 ttcttacgct tgtcttttcc acacggacta acaattacaa gcttttgttc ctctagcttt
    14281 tttatcatag tcgtcagcgt attattcgca agcccagtcg caagcgcgat atctgtcgca
    14341 gttgcgcagc cagtttcact attccataaa accgctaaaa tcttgccctg ttcaccccta
    14401 taaagagcct caggatcttg actcagtaac ttttgaaaaa tccgcccatt caacaaacga
    14461 atatgatggg ctagcaaatg actatctttc ataacacctc caatttattt cgatatcgaa
    14521 atgaataaaa taattgtaac actcatcgtt ctaactgtca actatttcga tttggaaata
    14581 atttttgata attatccaca ccaccatact ccggctcaac taacttttaa cgagagtttc
    14641 taaactcctt cgtcctccag tctacaaaag ccttccattc gtactatcct atattttatg
    14701 aggggacaca tttttcctat cagaccattt attttaaaga tagaagtaaa tcataattgc
    14761 ttccatctgt tcttttatag tatattgaag ttagactaga gcactgtatc ttctaaaaca
    14821 ttgatagaaa gcgatttgaa tttcccaatc aatttgttcg tatttatagc attttgaaac
    14881 tagaatagga caccatgact gctaaaagat ttctataaat tcatttgatt ttcctaatca
    14941 atttgttcat atcttatttc attccgctat aatttcacct taccctatct ttttcgtagc
    15001 acccttcaaa cagcctatcc cctaccgttt gacgattcct cacttcgctc cacttccatt
    15061 acagaagttt cttcactact atgggctcgg ctgacttctc atgattcctt gttactacta
    15121 tttgaacgct cacgagatag atcttacaaa aaatgctttg atccacaatg gaatcaaagc
    15181 attttaaaga gttcctcata cataagcgca gaagtcgcag ttcctctgta cttggcttct
    15241 tctcttttga taaagcgagc caagttgagc aactcaggtg ctggatgttt gggatttagg
    15301 agcaattcac gattgaccag gcctgagaga cgaactgcct gcaattgctc atttgtagta
    15361 ggcagttttt tagcagtctc taggagagca gcaactaaat cttcactcaa atcatgtcga
    15421 gcatgattgt aaagatcttt tataaggctt tctaggtttg gttctgccat ccctaccacc
    15481 tcccttatgg tttaataatg tttaatcaaa tcaaccgttg aacgatccaa tttcttcacc
    15541 aaggcttgta agaaagcttg cgcttctagg aagtcatcca ttgcatagag ggtttggtga
    15601 gaatggatat aacgagcgca gacaccgata gttgttgatg ggacaccacc atttttcaga
    15661 tgagctgcac ctgcatctgt tccgccttta ccacagtagt attggtactt gataccagct
    15721 tcttcagccg ttgtcaaaag aaaatccttc atccctggga gaagcaagtg acctggatca
    15781 tagaaacgaa tcaaggttcc atctccaatc ttgccttgac caccgtagac atcacctgct
    15841 ggtgagcaat caactgcgag gaagacttct gggtcaaact tggttgtaga ggtatgagcg
    15901 ccacgcagac caacttcttc ttggacgtta gaacccagat agagttcatt gccgagtttt
    15961 tgacctgata aagcttcagc tagctcgctt accatgagga caccgtagcg gttatcccaa
    16021 gcttttgaga tgatattttt ttcattggct gtcaaaattg cagaactatc tggtacgatg
    16081 gtatcaccag gacggatgcc aaaactttct gcctcagcct tgtccgcaaa accaccatca
    16141 aaaacgatat cggcaatggc tggcatggtt ggtcccccct ttccacgagt caaatgcgga
    16201 ggaacagaac ctgaaatcac aggaatttca tgaccatcac gagtcaagag tttgaaacgt
    16261 tggctgctaa ccaccatggg gttccagcca ccgatttcta cgacacggaa ggtaccatct
    16321 ggcttgattt cgctgaccat aaaaccaact tcgtccatat gagaagcgac caagacgcgc
    16381 ggtgcatcca cagcttctga atgtttgata ccaaaaatac cacccaagcc atctgtcacc
    16441 acttcatcca catgcggtgt caacttttca cgaagataag cacggacagg cgcttcatga
    16501 cctgagactg cagcaagttc tgttacttct ttaatttttg aaaataatgt tgtcatttca
    16561 gttccttctt tctttcatcc attttaccac tttttatagg agaaggatag tgggaaggtg
    16621 gatttctaag ttagtatctt agtcctgctc tatcttagaa aaggatagta ttctcttgca
    16681 tgtagtgcaa aatctagtaa acattccaaa attaactcga atatttattt ccaaacaaaa
    16741 aaacaataca ccatcaaagt tgtttgtatt tttcatgaaa tttacagaaa atagttgact
    16801 tccctttctt ctttctttaa atatatagtt ggttgagttt ggaatagtac gctgtagctg
    16861 ctgaaacatt tctagaaatt aatttgactt tcctaataga gttgttcata tcttatttca
    16921 atttactata gtacaaaact agaaaaggaa aaaatcatga ccaaaacaat tcttgttaca
    16981 ggtgggacta gctacattgg ctcccatacc gttaaagctc ttttaaatgc tggctataag
    17041 gtgcatgtcc tcgataatct ctccacagga aatcgagcgg ctgtggatag tcgtgctagc
    17101 tttaaagaac tggatgttta tgatgctagc gccttaaagg cttacttaga agaaaatcag
    17161 attgatgctg tcctccattg tgcaggtgaa attgttgtga gcgaaagtat tgaaaatcca
    17221 agtaaatact tcactgccaa cgttgctggt atgaaccaag ttctcaaagt cttatctgaa
    17281 gttggcattc aaaaaatcat gttctcttcg actgcttctc tctatggaaa taactgtatt
    17341 gacaagccag tgactgaaga taccctgctc gaccctgtca acccttatgc agagacaaaa
    17401 ctgatgggcg aacgaatgat ttactggatg gccaatcgct acgactggaa atatgttatt
    17461 ttccgttact ttaatgttgc tggggctgaa atggatgctt caaacggtct gcgtgtgaaa
    17521 aatccaactc acatcattcc aaatatcaac aagaccgcat tgggacaaaa tgatagctta
    17581 aaaatatttg gagatgacta cgatacacgt gacggttcat gtattcgaga ttacatttat
    17641 gtcttggacc ttgcataggc tcatgttaaa ggaatgaact acctatttca agaagacagt
    17701 tcttctcaaa tctttaactt aggaactaaa aaaggctata ctatcaaaga aatctttaaa
    17761 actgctgaag aattactaaa ttaaaaaatc ccacacgaga ttgttgctcg acgtgctagt
    17821 gacccagcca gtgtcctagc agatgcttca aaagctaaac aatatcttga ttggacggct
    17881 agctactccc tcgaagatat tattttatca gattaaggta aaaacatttt agaataggat
    17941 aacaaaggag aaagtcttgt tgctctctcc ttgttgtatt tattaaattg tcagaaattt
    18001 tattgacaga ttagaaaaat aatgttacat ttatatccgc agatatcttt cgataccaaa
    18061 tctacatgaa aggacggggt atgaaacttt ctcattattt aattggctta cttctactcc
    18121 tagtctttct ctctattagc attgggacca gtgatttttc atggggaaag ctatttgatt
    18181 tcgaccagca gacatggctc ctctttcaag agtcccgtct cccaagaact atcagtattc
    18241 tcctgactgc ctctagtatg agtatggcag gccttctcat gcagactatt acccaaaatc
    18301 agtttgctgc accgagtaca gttggaacga ctgaagccgc caaactggga atggtgctga
    18361 gcctttttgt ctttccatcg gctagtctga cccaaaagat gctcttcgct tttgtttcat
    18421 ccatcgtatt caccctcttc ttcctagcct ttatgaccat ttttactgta aaggaaaggt
    18481 ggatgttgcc tctgattggg atcatctata gcgggattat cggctcagtc acagaagtta
    18541 tcgcctatcg tttcaatctg gttcagagta tgactgcctg gacccagggc tccttctcca
    18601 tgattcagac ccatcagtat gagtggctct tcttaggcct catcatcctg ataaccgttt
    18661 ggaaattatc ccaaaccttc accatcatga atctggggaa agaaatcagc gagagtttgg
    18721 ggatttccta ctccctactt gaaaaactgg ccctctttct ggtggcgcta acgacaagcg
    18781 tcaccatgat taccgtgggt ggcctaccat ttctcggagt tatcgttccc aatcttgttc
    18841 gcaagcgcta tggagataat ctaagtcaaa ccaaactcat ggtcgcactg gttggtgcca
    18901 atctagttct ggcttgcgat atcctatccc gagttctgat taggccctat gagttgtctg
    18961 tcagtctctt gctaggaatc atcggtagtc tcggctttat cctacttctc tggagagggg
    19021 gacgaaaaga tgcagactaa aagcaaacat accaagctct tctggattct cattattctt
    19081 gccatcggag cttgtcttct ctacttttgg cccatcactc acttgtcagc ctttgcttgg
    19141 aagttgcgtt cccaaaagat catcgtttat ctcttggtag ccatcgcgac tgggatttcg
    19201 accattagtt ttcaaaccct gacggaaaat cgcttcctga cgcctagtat tttgggaatt
    19261 gaatccttct acgtcctact acaaacccta ctactggttt ttgaaagcaa gtttcttcaa
    19321 cttggcaaat cccctatctt agaattccta gtcttacttc ttgtccagtc cctcttcttt
    19381 ctcaccttac aaggttactt gaagacactg atgaagcaag acctggtctt catcctgctg
    19441 atctgtctag cgctcagaag tctctttcga aatatcagca ccttccttca agtcctaatg
    19501 gatccaaacg aatacgataa actgcaaaat agtctttttg cctcctttca acatctcaac
    19561 acttccatcc tagccatcgg ttctctgatc atcctcgctt tgacaatctt tttctttcga
    19621 aaagcagtcg ttctagatgt cttgcacctg caaagagaaa cggctcagat attgggactc
    19681 gatgttgaaa aagaacagaa agagctcctc tggggaatcg tgcttttgac ctcaacggcc
    19741 actgccttgg taggacctat ggccttcttc ggctttatgc tggccaacct cacctacctg
    19801 attgtcaaag actatcagca caagttactc tttatagtgg ccattctggt tggatttatt
    19861 agcttaacct tggggcaagc cttgattgaa cgagtctttg cactggaaat tcgtatcagt
    19921 atgatcattg agagtgtggg tggcttctta ttctttatct tactatatag gaggtctcgt
    19981 cagtgaaact ggaaaacatt gacaaatcca ttcaaaaaca ggatattttg caaggcattt
    20041 cgcttgaagt cagtcctcaa aaactgacag cctttattgg tccaaatggt gctggaaaat
    20101 cgactctcct ctccatcatg agcagactaa ccaagaaaga tcagggagtt ctcagtatca
    20161 aaggacgtga aatcgagagc tggaattcgc aagaactggc ccaagaacta accatcctaa
    20221 aacagaaaat caattaccaa gccaaattga ctgttgaaga actggtcagt tttggacgtt
    20281 ttccctacag ccgaggtcga cttagatcag aagactggga aaaaatccga gaaactctga
    20341 actatttgga actgaccaac ttaaaagacc gctacatcaa tagcctgtca ggggggcaac
    20401 tccagcgcgt ctttatcgct atggtactgg cccaggatac ggactttatc ttgctggacg
    20461 aaccactcaa caatctcgat atcaagcaaa gcgtcagcat gatgcagatt cttcgacgac
    20521 tggtggagga actcggcaag accattatca tcgtcctcca cgatatcaac atggccagtc
    20581 agtatgcaga tgaaattgtc gccttcaagg acggccaggt ctttagcaag ggaagcaccg
    20641 atcaaatcat gcaggctgac ctgctcagtc aactttatga gattcccatc acgctagctg
    20701 atatcaatga caaaaagatc tgtatctata gctagtaaca taaaagctca agttagagaa
    20761 ccttcagtct cttagtcaat aagattaaga gactccctaa atcgttatca cattttaaaa
    20821 aggagaaatt atgaaaacat cccttaaact ttatttcact gccctagtgg ccagcttctt
    20881 gctcctactt ggtgcatgta gtacaaactc aagcactagt cagacagaga ccagtagctc
    20941 tgctccaaca gaggtaacca ttaaaagttc actggacgag gtcaaacttt ccaaagttcc
    21001 tgaaaagatt gtgacctttg acctcggcgc tgcggatact attcgcgctt taggatttga
    21061 aaaaaatatc gtcggaatgc ctacaaaaac tgttccgact tatctaaaag acctagtggg
    21121 aactgtcaaa aatgttggtt ctatgaaaga acctgattta gaagctatcg ccgcccttga
    21181 gcctgatttg attatcgctt caccacgtac acaaaaattc gtagacaaat tcaaagaaat
    21241 cgccccaacc gttctcttcc aagcaagcaa ggacgactac tggacttcta ccaaggctaa
    21301 tatcgaatcc ttagcaagtg ccttcggcga aactggtaca cagaaagcca aggaagaatt
    21361 ggccaagcta gacaagagca tccaagaagt cgctactaaa aatgaaagct ctgacaaaaa
    21421 agcccttgcg atcctcctta atgaaggaaa aatggcagcc tttggtgcca aatctcgttt
    21481 ctctttcttg taccaaacct tgaaattcaa accaactgat acaaaatttg aagactcacg
    21541 ccacggacaa gaagtcagct ttgaaagtgt caaagaaatc aaccctgcca tcctctttgt
    21601 catcaaccgt acccttgcca tcggtgggga caactctagc aacgacggtg tcctagaaaa
    21661 tgcccttatc gctgaaacac ctgctgctaa aaatggtaag attatccaac taacaccaga
    21721 cctctggtat ctaagcggag gcggacttga atcaacaaaa ctcatgattg aagacataca
    21781 aaaagctttg aaataagcca cttcaaactc a
//
LOCUS       DAVBHS010000016        21154 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVBHS010000016 DAVBHS010000000
VERSION     DAVBHS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21154
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.016"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(295..2022)
                     /gene="ezrA"
                     /locus_tag="VQ651_000438"
     CDS             complement(295..2022)
                     /gene="ezrA"
                     /locus_tag="VQ651_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HET2753480.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2104..4050)
                     /gene="gyrB"
                     /locus_tag="VQ651_000439"
     CDS             complement(2104..4050)
                     /gene="gyrB"
                     /locus_tag="VQ651_000439"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET2753481.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4065..4628)
                     /locus_tag="VQ651_000440"
     CDS             complement(4065..4628)
                     /locus_tag="VQ651_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2753482.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4713..5267)
                     /locus_tag="VQ651_000441"
     CDS             complement(4713..5267)
                     /locus_tag="VQ651_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET2753483.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5278..6501)
                     /locus_tag="VQ651_000442"
     CDS             complement(5278..6501)
                     /locus_tag="VQ651_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET2753484.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVSLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6538..8988)
                     /locus_tag="VQ651_000443"
     CDS             complement(6538..8988)
                     /locus_tag="VQ651_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HET2753485.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQAMGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIIGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9187..10116)
                     /locus_tag="VQ651_000444"
     CDS             complement(9187..10116)
                     /locus_tag="VQ651_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2753486.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10256..10543
                     /locus_tag="VQ651_000445"
     CDS             10256..10543
                     /locus_tag="VQ651_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HET2753487.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10577..11911)
                     /gene="ciaH"
                     /locus_tag="VQ651_000446"
     CDS             complement(10577..11911)
                     /gene="ciaH"
                     /locus_tag="VQ651_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HET2753488.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11901..12575)
                     /gene="ciaR"
                     /locus_tag="VQ651_000447"
     CDS             complement(11901..12575)
                     /gene="ciaR"
                     /locus_tag="VQ651_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HET2753489.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12684..15230)
                     /locus_tag="VQ651_000448"
     CDS             complement(12684..15230)
                     /locus_tag="VQ651_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET2753490.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHIAYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15354..15794)
                     /locus_tag="VQ651_000449"
     CDS             complement(15354..15794)
                     /locus_tag="VQ651_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HET2753491.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15769..16638)
                     /locus_tag="VQ651_000450"
     CDS             complement(15769..16638)
                     /locus_tag="VQ651_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HET2753492.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFTELTNTEANCLVGTKEFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDLESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16680..17408)
                     /locus_tag="VQ651_000451"
     CDS             complement(16680..17408)
                     /locus_tag="VQ651_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2753493.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(17416..17895)
                     /locus_tag="VQ651_000452"
     CDS             complement(17416..17895)
                     /locus_tag="VQ651_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2753494.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVDHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVK"
     gene            complement(17906..18604)
                     /locus_tag="VQ651_000453"
     CDS             complement(17906..18604)
                     /locus_tag="VQ651_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HET2753495.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18597..18863)
                     /locus_tag="VQ651_000454"
     CDS             complement(18597..18863)
                     /locus_tag="VQ651_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HET2753496.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(18929..19855)
                     /locus_tag="VQ651_000455"
     CDS             complement(18929..19855)
                     /locus_tag="VQ651_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HET2753497.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19930..20745)
                     /locus_tag="VQ651_000456"
     CDS             complement(19930..20745)
                     /locus_tag="VQ651_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753498.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20749..21069)
                     /locus_tag="VQ651_000457"
     CDS             complement(20749..21069)
                     /locus_tag="VQ651_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET2753499.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLTKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         6398 a         4642 c         3705 g         6409 t
ORIGIN      
        1 aagttacttc aaagtacaga tttgaagctg cagataagac tgacgaaatc actttaatac
       61 taaatgaaaa tcaaaaagca aactaagaag ctagcctcag gctgtacttg agtacggtaa
      121 ggcgacgctg acgtgatttg aagagatttt cgaagagtat aaaacactgt tttgaagtgg
      181 tggataaatc ggctgggtaa aaactcaatc tcaggagaaa atgaagtaaa tctccccaca
      241 actatagaaa aagatttgat tctgctcctc acacaataaa atctttttct tttattaaaa
      301 acgaatcgtt tcacgtgttt tctcatatga ggtaacaaag cgattggtta caccaggctc
      361 tgccacttcc aatgcttgag aaatcttgtc aaatgaagcg tgataatcaa attctttttc
      421 aaaaatatct aaagcttcgt taaatgcttc ttgaatgcgt tcatcaaatg agcgatagcg
      481 gttagaatat tgcaagagtt gctctgtcaa agttgcatat tgtacaatat tataagtttc
      541 cgtttctaaa gcttccatat cattcgttgc aatttcaaga actcgggtaa cagattcaat
      601 gttaatcatt ttttgttcta actcaaccat taaatcctcg gtattattgc ttgccgtaaa
      661 gaataacttc aagaaagttt gtggaatacc tggcagattg cgtttttcca tgtatcgctt
      721 gatagtatgg agacgattga cataaacatt ggccttttga cgtgcattaa tatcatcttt
      781 ctcaatttgt gtcaggcgct cactaactga aatttgctca tcttcaatat cttttagttg
      841 agtttgtaaa tcctcaagat tttcttcaag aactgaataa gcttgggttg gttcttcttg
      901 atttgaagtt acctcaacaa tagctgcctc aaaactctct aattctgtct gaatacgacg
      961 aacatggctt gcagctgtct caggaagtaa ataggtcttg ttcaaacgtg caatatcttc
     1021 tcccaataaa gtattattct ctttcatatg ttgaaggtaa gttggaagag ttgcaagtag
     1081 attttccact actttctgag cagcaatttc tcgagtaaaa atatcataca aggcattgat
     1141 ttcctcttgt gcctgtccat tctcatattc ggcattatcc aattccaact gacgaatatt
     1201 ctcttggttt ttcttgaatg cttcataaag caagtggaaa cgcgcttcaa tatccgtttc
     1261 aacaaaatga taattagcat caattagttt acgataaccg gcttccaaat cctgtaattg
     1321 atctggcaat tctgtagaaa gcgtcgtaac caaggctgga acacgatcca caatatgact
     1381 taaggccaaa atgtgatttt ctgtattatc caaaatcact gcggcttcca cagggtcacc
     1441 cgatgaattc aaggttacaa attgtgaaaa ttcagattgg atattttcta attgtttttc
     1501 aatttcatcc aaggcttgac catactgttc tgaattttca gcaactctat gctgaagttc
     1561 ctcaaataaa tccaaagcat gaagaacacg accactattt ttagattctt gcttctctaa
     1621 gtctgccaaa gcattgcgaa ttgccgcaat atcttcttca atcaaagtaa tttgactctc
     1681 aatttggtca atttgatgac tggccttgag aaaacgaaat gaatggttat aaccttctgc
     1741 ttcaaagaga ttattttcaa tatcggcaaa agagttgaga gataaatcga cccatttttg
     1801 attccattca cggaaagcca cttgactttg tccaatcaag tgcatatttt ttacagcttc
     1861 tacttcatca tttactggaa gattgtatag ttcttctttt ctttcttcta gcgcctctaa
     1921 tctcccctcg tttcgcttac gtagaaagat tgccactaca taagccagaa ctaaaatgac
     1981 tgcaattgca accattaaat aaattagttg tccattagac atatcaaact ccttttttac
     2041 ttgaaacaat cgtaacgatt atatcatatt ttttagacca agggaactac ttctccctat
     2101 tttttagaca tcaagtgtac tatagacagc attttcttcg ataaactcac gacgaggctc
     2161 tactcgatcc cccatcaaca tatcaaagat tttatctgct tctgcagcat catctacaga
     2221 aactctagcc atcaagcgat gttcgggatc catggttgtt tcccacagct gatgatcgtc
     2281 catttcacct agccccttat aacgctgaat agtcggtttg gtacgacctt cactataacg
     2341 ggctaaagct tcttggagtt tgatttcttg atctgcaccc ggctggatat attctttaat
     2401 ctcgcttcca accttgacac catagattgg tggttgggca atataaacat aaccagcttc
     2461 taggattggt ttcatataac gataaatcaa ggttaaaaga agggtacgaa tatgggctcc
     2521 atcgacatcg gcatcggtca tcaaaacgag tttttggtaa cgggctttcg aaacatcaaa
     2581 ttctgcgcca aatcctgttc ccatggctgt gaaaagacta cgaatttctt cgttagctag
     2641 aatcttatcc atacttgctt tttcaacgtt caaaatctta ccgcgaattg gaaggatagc
     2701 ctgaaactca cggttacgac cagatttggc tgatccacca gctgagtctc cttcgacgat
     2761 gaagagttct gtttcagcag ggttattaga agaacagtct gctagtttcc ctggaaggtt
     2821 ggaaatttcc aaaccagatt ttttacgtgt gacttcacgc gcacgcttgg cagccacacg
     2881 agccttggca gctaaaatcc ctttttccac gatacgtttg gcaatctgtg gattttccat
     2941 gaggaaatcg gagaaggctt cactgaagag gcgattggta atcttgacca cttcgctatt
     3001 tcccaatttg gtcttggttt gtccttcaaa ctgtggattt gggtgtttaa ctgagataac
     3061 tgcagttaat ccttcgcgaa catcttcccc tgttaaatta tcttcattgt ctttcagtaa
     3121 cttattctta cgagcataat cattgataac acgagtcagt gccgtacgga aaccttgttc
     3181 atgcgttcca ccttcatggg tatgaatatt attggcgaaa ctcatgacat tttcatggta
     3241 acctgttgtg tactgcatgg ctacctcaac tgtgatatca tccatctcac cgtctgtata
     3301 gattggtgta tcaaagatta catccttgtt ctcgttgata tattcaacgt aactagcaat
     3361 cccaccttca taatgataat gcttggtttg ttccaaacct tggcgcttat cagtgataga
     3421 aatttgaaga ccgcgattta gaaaggccaa ctcttgaatc cgtttattta atttatcaaa
     3481 atcaaaggtt gttgtttcag taaagatttt tgggtccggt atgaagtgaa ccgttgttcc
     3541 agttctatct gtatccccaa ccacctcaag gtctgcaaca acatgaccac gacggtattc
     3601 ttggtaatga atcttaccgt ttttatggac atgaacgtct aattgagtgg aaagggcatt
     3661 aactactgac gaccccaccc cgtgaagacc acctgaaacc ttgtatccac caccgccgaa
     3721 ctttcctcca gcatgaagga ctgtaaagac ggtctcaaca gcaggacggc ctgttttttc
     3781 ctgaatatcg actgggatac cacgcccatc atccacaaca gtaatcgaat catctggctc
     3841 aataaaaact tgaatatggc tggcaaatcc tgccaaggcc tcgtcaattg agttatcaac
     3901 aatttcccag actagatggt gaagaccttc ttttgaggtt gatccaatgt acatccctgg
     3961 acgcatacga acagcctcta agccctctaa aacttgaatt tgactggcat cataatcctg
     4021 tgcctgcaga tttttgattt cttctgtcat cttattcctt tttcttatat gtctaatact
     4081 tgtcttaaat tcacgatact ggtaaacaag tgggtatcaa gtcctgcagc ttgacctgct
     4141 tcgatatcaa tcggccgatc accaatgaca agaccagagc taatctgata cttttctctt
     4201 aaataaagca tggattcggg atttggcttt ctcttaaagc ctgagctaga agtcaccact
     4261 tctgtaaaat aagctgctat agaggttttt tctaaaattt ccaaaacctg atcatttcga
     4321 tgagagacca aaaaatgacg gccaccttga tttaaaatgt cttccaatag gtcagaaact
     4381 ccttcaaata aaatcgggtg ttcaagctct ctggcttcat tttccttgta cttttctaaa
     4441 aaattctcta aattgggagc gaatgtctca atcgcaaaag gagtagaaac ctttaaagct
     4501 tgatagacac tgtcatggtc ttgtgtgata ccatacagtg ccaatgtttc aacaaatgca
     4561 gctgttgaag tttcataatt atccagtaaa gttccaccta aatcccagat gtaatcgtga
     4621 tatttcatac ccttcattat agcatttttt tatgaaaaaa gcgatgattt ccctgagttt
     4681 tccacataaa aaacgagaga ataactcccg ttttactgat ttttaccaaa gacatctcga
     4741 tagagcattc ctgttcgtaa actctctgcg tcccctccta gttgctccac caactcgtag
     4801 gcaaaggcaa gggctgttga aggaccccga ctggttgtca actgaccatc taccactact
     4861 gtttccttga cgtagtgacc atcaaggatt tgctcttgaa cgccgtcata acaagtgtat
     4921 cgcttatttt tcaatatctc tgcttgattg agggcaattg gtgccgcaca aatggctgct
     4981 agtttcttcc cttcttgctc gaagctttgc aattcttgaa tcaaggtctg attatcacgt
     5041 aaatgtgcag aaccaggcat acctccagga agaacaatca tatcatagtc tgataaatct
     5101 ccatcaaaga catgatctgc tcttacttgg attgcatgcg aacccgttac ttgctcttca
     5161 aaaccaacca tatcacatgt gatattggct cgacgcaaga catctacaac tgtcaaggct
     5221 tcaatttctt caaagccctg agctaacata actgctactt ttaccatgat tttctcctta
     5281 tttaatttgt tttaatacaa cctttttccg tttgaatggg acttttccgc tcttttcttc
     5341 agctagatta gtctggtaac tcatcgataa aagcaaacca acaccaatca gattactgat
     5401 aatagctgat cccccttgcg aaatgaaagg caagggaatc cccgtcaaag gaagtagtcc
     5461 agtcacagca ccgatattct caaagatgtg gaagagcaac atcataatca aacctgtgga
     5521 aatataagtg tagaactggt tatttgattt aagagtaatc ttcaacatac ggtaaatcaa
     5581 catgagatag agggcaataa ccaggacaga gccaataaag ccaaaatctt ctgcaataac
     5641 cgtaaaaatc atatctgact ctcgaactgg gataagcaga ttcgaagcat taaatccctg
     5701 accaaataag ccaccactcc caatggcaat ctgcccttga gcctgctggt aagtcgttgt
     5761 ttgggcaaac tcaaagggat tgagccaagc caaaatccga ttgatttggt aggtcggcat
     5821 tccaatctgg tgaagaaaag ctcgtccgtc cttgctaata aagatagcta agaaaccagc
     5881 aactcctgtt acagcagtca caaatactgg gataataatt ttccaagaaa cccctgataa
     5941 taaaacgatt cctgagaaaa tggctacaaa aaccaaagcc gtccccaagt cactttgaag
     6001 tgctaaaaga actaggactg gaatggtaaa gagaatcatc cagaaaatta acaaaaagtc
     6061 cagcgaaacc gtgcgtctcc attccttatg tttctttgta aattggacaa tgacacgagc
     6121 caacatgagg atataggata tcttcataaa ttctgacggt tgaaataggg taattccatt
     6181 tattgatacc cagtttttgg cacccgttga tgcaactaag cttggattat aaaatacaat
     6241 cggcaagatc ataagtccca agcctaaaat atatagaaag ggggtcacct tccaaagaaa
     6301 ttctgtatta aagagcatga ccacaaaacc aatcacaagc cccaaggcaa tccaggcgac
     6361 ctgctgccct aaaatgggca gaatattatt gggataatca tgactaacgg ctatatagat
     6421 agccaccaca ccgatgacca gtagaaaaaa tactggcaag agcaaactat aatcgactct
     6481 agagtcgaga gaacgtttca tatatactaa ccttatactt tcatacaata ctatttatca
     6541 aagttcatta aaaaatctat caatagcctc gtcaacttcg gatcgagaga tggttttaac
     6601 agtcgcttct tctgctagag atgctactat ttgtttgccg tatcgttttc cgatgattct
     6661 cctatccaaa ataagagtta aggaacgttg gtattcacgt ctcatacttc ttcccaaagc
     6721 ctgttttaaa cgaataatgg ccattggcaa ttgataatca taaaaggcat ttttcccttc
     6781 ttgattcagt tcttgattaa tctttttcgt caagggttct tgaggatttt ggaaaggaag
     6841 cctcggtaca acttgaatca caaaaggatg gcttgaaaaa tcaactccct cccagaaact
     6901 tgctgcacca agcaagattt gttgttcacc tttttcaaag cgtttcttta gctgatgaac
     6961 atccccattt ttatactggg ccaagtggct aactgtaagt aaatccgata ctgctagaag
     7021 catgtcttta gcggtaaaga gaaccaaaat cggttgctgg aaagcttgaa tttccactag
     7081 taaagcagct acctctctgg catagacttc taaggaggtt tctgttacca gagggaaatc
     7141 tttgaccata accacttctt gttcctgttt tccccgagat tcaatcttga caaatttggc
     7201 ttcaggatag cctaaaaggt ctgccaaaga aaccctctga ctaatctcaa gagtagccga
     7261 tactcccatg gcttggcaac tctcaggaag taaagaggac agaagagtac ggcctgattt
     7321 tgtagaagaa atctgaattt tcttttgact cgtttcagtt acttcaagcc agtaatcacc
     7381 ttcagctgta aaatagcgaa ccagctcatc aaagccttct acttctaatt ctgaaaaata
     7441 ctgatggaga ttgtccagag aatctaagat atttttccta gatttaccag actgaaattg
     7501 ttctatcaag tagagacact caaagcgaat actttctagt atccgttgtt gaaccctgtt
     7561 ttcttctcct actaaagcct tatcaattaa atcgataata gattgtatat cgtaggtctc
     7621 ttgaagcaga ttttctagag ctaacaaaat cttttggact tcatcaataa tcagtaaacg
     7681 gtcactgaca aattcaggat tatcttcaag tctggtcacg agataggcat gattagtcac
     7741 taaaagcttg caagtctctg ccctttcttg actacgtttc caaaaatctt ccgtcacaaa
     7801 taagctctgg gatgataaat tcccatcatg acgaaggtct gctagaaaat gttggtaacg
     7861 atagagttgc ccgatttcat ccaaatctcc tgtctctgtc tcagtaagcc agaccaagac
     7921 ttgcatttta aagcgtctaa ataagcgatt ttcatcattt tcctgcaagg aatgataaaa
     7981 ggcatccaac ttcagataat tttgtggtcc ctttaagcta tgaatatctg tatggaacac
     8041 ttccttgagg cgtttacctt cttcttccat gatttgattt tgaagaatct ttgtcggaac
     8101 actaagaaca atttgtcgct ctttggattg agataaagcg ggtaagagat agccataggt
     8161 tttcccaatc cctgtcggcg cttgaatcag agagacaggt tcatctttca atagcaagcc
     8221 aacctcttta gcaaaacttt cttgttcctc cctcacttca aggttcaaca gagaaatatt
     8281 tttagaaaag tcttgagata gttttcgtgg ctccagagaa gctgccgttt tcttaaaata
     8341 cagaccttga acttggacca agtctggaga actcaggata gattggttgc gataagtttc
     8401 ctcaataacc aggtaggact catataggag agcgtcagcc atttccagca agcgttccaa
     8461 gagaccttta ggaagctgag tcatcttttc ccgtaaaaaa agaagtaatt ctgctgtagc
     8521 ttgggcatct gaaagggctg tgtgtgcgtg tttaagagga attcctaatt ctcgacacaa
     8581 aatcggcaag ctatattttt ccagttcagg gaaaaagacc tgggccaatt cgaccgtatc
     8641 aacacgaggg tttcttagct catagccttc aaaaaataaa ttttccgcca agagattagc
     8701 atcaaactga acattatggg ctacaaaaat cccatcctcc accaagtcaa atatttttct
     8761 ggcaacttgc gaaaaatcag gtgcttgcgc cagacgttgg tctgtcaatc ctgtcagttc
     8821 tttgatatga gcatccaagg gttcatgtgg attgacatcc gtcgtatagt gatcgacgat
     8881 ttctccgtcc tcaatcacga caattcccac ttggataatt ttagccttac tacctgtgct
     8941 agttgcctct aaatccacca caacatagcg attaccaatc gttttcatta taaaaaatta
     9001 taccaaaaaa agggccattt ggcacctgca aacataggat aattttcaaa ctcaagaaca
     9061 ctacttttct taaaaatccc atcgaataga gcattctagc ctgctgattt tttaaaatag
     9121 tgaagaaata cgaaaaattg agagaagaaa tgttttcatc ttccttctct caacgaatca
     9181 tgtattttat ttaaccatgt caggatcgta gtcataaaat cctgtatcaa ggaaacggtt
     9241 tttagggtga agtttacgaa cttcttcatc cagcaagaag gcttggtcat ggctaaagtt
     9301 gccctcttga atcaagctac gcgcttggat aaagagtttt gcaatctcaa caaagttctg
     9361 accaggagtg tagagccctt ctagtttcca gcgagtaaag ccatgctcca ccaattctgt
     9421 taatttgatc atcaaatcaa ggtcattgtt ggcaaagata tgggtcccat gattatcttc
     9481 aaaaatggaa tagtggctct ctggatcact tggctcagcc aagaagaggt cacgtttatg
     9541 cgtcttttca tcatcgatat gtgtaaagtt atagtagttt tgcaagagtg gacgtttaga
     9601 atgatggatg acgctagcac cgtaaaccaa aacttcagca ggaatttcca aaatctctgg
     9661 cattttgaaa agttcagctg atggaatttc acgcgccaaa acagcctcag atgcgccagc
     9721 cttttgtccc cagaagttaa tctgacgact gctagttacc atggttgaag catcgtagat
     9781 ggtcttaaat gaataaccat cgcggttaac tacgtaaaag acgcctgcat ccccaatcgt
     9841 aatatagtct gtcttgattt cttccaagaa gtctaagaaa ggcttgatac ggtccatcat
     9901 atcttggtgc atgagagcat tgaccgcaac gagcaattcc ttaccagcat catgaaccaa
     9961 cttagcgatt tcacgtaatt ggtcataact aaaggtcgtt ggcagacgaa ggccaaaatc
    10021 tttctcaccg acatagatac ggtctacgcc agcttcgagt agttgttcaa cttgttcaat
    10081 actttcagca gttgctgtaa tgacaatctt ttccataaga aaaattatac cacatttctc
    10141 caaaatcatc catgctttga aaatgaaaag ggactttatt ctttttgtaa cctttctgta
    10201 ataattctct gccgaaaaaa tgataaaata ggtatgtact gttaaggaga gaattatgcc
    10261 cgtaagaaaa ttacaatcct atgaggtaga ctatcaagaa gaattaaacc agcagcttcc
    10321 tcattaccaa gcttatacac ctgaagcgca atctgatgcc aatctcaagg aaatcctatt
    10381 ttttattaat atcgctgttt tttgtatctg tattgctatc tttagtttta tctttttagc
    10441 attaaaatta tcaactgctc ttgcctttgc cgcagcaatc ggattcagct tacttgtttt
    10501 aaaagttcaa cggtctatta tcaaacgaaa acgtagaaga taaatccaaa atatcgcccc
    10561 agttggagcg atatttttat tttttctttt tagatggtgt ctgaatggca atcttcactt
    10621 caaagattgt tcccttgggt ttattatctt tgacagtaac agttcctttt agagcatcta
    10681 caatttgctt ggctagggat aatcctaaac caaaaccacc tttttgccga gttctagcct
    10741 tgtctactcg ataaaaacgg tcaaaaattt tctttttatc ttctgtcgaa ataccgattc
    10801 cattatcaga aacaagtaaa taaagattgc gatcggtcgc cgagataaga aaatcaattt
    10861 caccatcctc ctcagtatac ttgacggcat tatcgaaaag aatggtcatc agttgtttca
    10921 gaagaagctg atctgtgaca attgttcgat ggatacgatt ttcaaaacgg aagacacgat
    10981 tattttccga agcaatcatc tcgtagtttg tgaaagttgt attaaaaaag ctagttggaa
    11041 cttctgcaag ctccggctta atcccatcat ctctccgagc taagttcagc aagcttgtcg
    11101 ttaaaaaacg catatttcgg acttcttcca aactcgatgc aatgctttcg ctcacatcca
    11161 taatggtagc ttctggctta cgaaaaaggg tctctaagcg attttgcaaa actgcgagtg
    11221 gagttcgtaa ctcatgactg gcattttcca caaaagactg ttgcttctgc atactctcaa
    11281 gcaggggcct aacactgacc ctagctagat agagactggc aagtaaagac aaaatccaga
    11341 aactagccat cacgaccaca atcaattgct catgcttttg actagcctgt tccaactgac
    11401 tggtattaat caagacagca gcatacttga tattggttga aaccgaacta atattggtct
    11461 ccatcaaaat cacacgatag atttcttcct gcccatagct attaaaaacc tgaatctggt
    11521 agatatgtcc tagttctttc ttttctaact taatcttatc caagcccaaa aatcgatttc
    11581 cagaaagaag ttgagtaaag tctttatcaa agagaatgac ttccgtattg gaactgacat
    11641 taggttttat ttctacttta ctagcgtccg ccctagcatt ttctaaatct ttaatctctt
    11701 ctgttgccct atttatagcc agctgaataa ctgcttgagg attttcactc agtccatgaa
    11761 gcttatcgtc caccgaagta taaagactcg aatgcatgac ttgtaaaata atcagagtca
    11821 ttgtagaaaa aatcagggtg aagacaccga agttgcggat aaaataacta aagtcatccg
    11881 cataccatgt ttttttaagt ttactgaaca tcttttaaaa gatacccaac actacgcaaa
    11941 gtttgcaaat tctctgcaaa agtggttccc tttaatttct tacggacttt tgaaacatag
    12001 acttcgacaa ccgaaatcgt tgtatcacta tcaaatcccc atagacggtc aaaaatctgc
    12061 gtcttaggca aaatcacatt ttgattttga aggaaataaa ctagtaaatc gaactctttc
    12121 cccagcaatt cgacaggagt atcttcaact ttaacggtat tggttgataa attaaccacg
    12181 atattcccat aagtcaaggt gttttcatta aacttccctg aacgtttgag aagggcctga
    12241 atccgcattt taagttcttc taggtagaaa ggtttggtca gataatcatc cgctcccagt
    12301 tcaaatccat gtcccttgtc atccaaactt tccttggcag tcataatcag aactggtgtc
    12361 gtaattccct tttcacgcaa ttcttttaag acttggaaac catttttttc tggcaacatc
    12421 aaatccagca aaatcaagtc atagacacca ctctcagctt cgtagagacc ttcttctcca
    12481 tcaaatacct gcataacatc cgcaaaatcg tctaaaaagt caaatactga atttgacaga
    12541 cctaggtcat cctcaaccaa taagattttt atcatgagaa actcctcctt attaaaacta
    12601 ttataccaaa tttgccttaa aaaaaactca actctctgca ttttacatga gatagctgag
    12661 ttttcttttt attttaggct tatttatgca tttccgtatt gaagaacaac tgcttcgact
    12721 gcagcttttt cacggctaat caagtcaaca cgcgctgcaa tttccttgat tcccataccg
    12781 atgttacggc taagagcaag gtcagaaagt tgcggctcaa agaactcctt gtattccgcc
    12841 aagcgttgct gagtcttaaa tacatgagca ggaaggataa caaagctatc aaagctcata
    12901 tctcctccaa gggctgcctt aatccaagcc cagttttcac gcgcccaaga ccaagctgtt
    12961 ttctgagttg cttgatgagc taggaattgg taataccaag cagacaagtc ctgtggtttg
    13021 accacaaatt tgtccttcca agaagtaatc aagttttgga tattatccgc atctgtactg
    13081 tatgcaagag ctgctgccaa ctggcgttta aagacagcat ctgttgcgtg agtataagta
    13141 tcaagataaa gtgctaacaa gtctttagtc tcatgatgtt tcatctcatt aatcagaact
    13201 tgtgaacgaa tagctgctgg gagtcctgca agattctcct tgtgtgttgc gaagatttgg
    13261 ctagcgactt gactagcttc tgcatcattt gagcgaatca tcatcgaaac agccagctga
    13321 cgaaccaatt catcctcatc tgattctccg tctttagctt caaaaccaag acggtcatag
    13381 ttatgacgag ccaatttagc aaccagtcct ttgaaggctg tttcagcatc cgttccttca
    13441 tcaataaagc gctcaagggc tgaaatcact tgagaaacag ctgaaaccac cagataagac
    13501 tcttccttag caagtttatc aagaactgga agcaagtctg cataagcaat gtgccctgcc
    13561 tcagccaaca aacgacgttc ttgaacaatt tgcagtttgc ttgtgttatc aagtgtctct
    13621 agctcagcaa gaacagcttc taacaagtct ccttgatagt cggtaatata gtgggcagta
    13681 ttttcagtgt tgagacgaag agctccttca ttttcagcaa gaagagctgc gtagccaggg
    13741 atttcgatac tttcagtttc gagtgtatca ggcaagcctt tccagttgct attgaggggc
    13801 accacccaga gacggttctt gtcttcgttc tcaccgatga agaattgttt ttgtgaaatc
    13861 ttcaagacat cattttcaac tttaacagta agaactgggt aaccaggctg ttccaaccaa
    13921 gaatccatga aggctgcgac atcacgtcct gacgcttgac caagggcatc ccaaaggtca
    13981 ctaccaatgg tgttgctgta ttggtgtttt tcaaagtagg cgtgcaaacc tttagcaaaa
    14041 tcagcatctc ctagccaacg gcgaagcatg tgcatgagac ggcttccttt ggcatagacg
    14101 atagcgccgt caaagagtgt attgatttca tctggatgtt taacttcgac gtggacagac
    14161 tgaacgccat cagtagcgtc acgttcaaga gcaagaggta ctccacctgt ttggaaatct
    14221 tcaaagatat tccagcttgg ttcgatggta tccacacaga cgtattccat catattagcg
    14281 aaactttcat tgagccaaag gtcatcccac catttcatag tcacgaggtt cccaaaccat
    14341 tggtgagcca attcatgggc cacaacaagg gcaacttgtt gacggctagc aaatgtagag
    14401 ttctcatcga caaccaagta aacttcacgg taggtcacaa gaccccagtt ttccatagca
    14461 ccagctgaga agtcaggaag ggcgatgtgg agagattgag gaattgggta cttaactcca
    14521 tagtaatctt cgtaaaactc gatagagcga acagcgatat ccagtgagaa atcaagattt
    14581 gaaagtggat gtgctttggt tgagtagaca cctaccaggg taccattttt agttttagcg
    14641 gtcacccctt gcaaatcacc agcaacaaag gccaacaagt aagaagacat gcgaggtgtt
    14701 gtctcaaact tccagatacc tgtttcctta cggttttcaa catcgatttc tggcatgttt
    14761 gacaaggcca attcaccttc tgcttggtca aagcgaagag agaggtcaaa agttgctttg
    14821 gcttcaggct catccacaca tgggaaagct tcgcgcgcaa aatggctctc gaactgagta
    14881 gacaagacct ccttcttgac tccatcaact gtataataag aagggtaaat ccctgtcatg
    14941 ttgtctgtaa ttttaccaga aaaggcaaga accaattcaa cttgaccagc ctcagccaat
    15001 tcgatatgaa gggcttcatt gtcatggtca actgtaaatg gacgagcttg acctgcaact
    15061 tctacagagg tgatttccaa gtctttttgg tggagggaga tgcggtcact ctgtgcttga
    15121 ccagtgatgg tcactttccc agaaaaagtc ttggtctcac gactcaaatc taaaaataaa
    15181 tcataatgtt caggaacaaa ttgcttaata aaatgttcaa ctgcttgcat agttttctcc
    15241 tattctaagt ttaagagcta ctgctcttct tcaaacaaac ttattatatc atattttgct
    15301 tgagaaaaaa ggattggaca gcgatttcca aaactttctt ccttctaaat agtctacagt
    15361 gggtagacct tgcgaaattc ttctaaaaca accttgcttt caggtgtaaa agtcagccct
    15421 acttcaccca aacagtctgt gaaaaagtcc tcatgaacct ggcgccaata ggcaagtgat
    15481 ttgtctccct caccttcctt aaaggcatgt tgcgcagaaa cttgattgaa gagttcaaca
    15541 gaaacctttg taatttcgac aatgcagaca gactgatttt gactatctaa aatgacatcg
    15601 aaggtcccta cttggggaag tggttcgtct tctagtacat agaggtcata ggctgatgct
    15661 gttgctgtct tttctccttt aaacaccaaa tccgctaaaa ggtctggttc aactccaaaa
    15721 gcccaagcat cgatttcatc tccgatcaaa ggattgattt gcttgtattt attccacatt
    15781 tcttgcggta tcatgggtac tcctttgtaa ttttttactt tcttctttta tgtgtttaag
    15841 atgatctgga tggtcaatct ctaaatcaaa aatctctgga atagaactgt agtggataat
    15901 gcacttgata cccaactgat tcattttttg tatgaaagaa gtattcagat agcctgctac
    15961 agcaaaatta atcttgttct ttcttgcttt atcctgcata tctcttagca tatctaacat
    16021 tattggactt tccagatcat gccattgact gtttctcata gtcgcaaaaa caaaggaagt
    16081 caaatcattc attccaacta caatctttga aatgcccgtt tccagtatac tagataagtc
    16141 aaaatacgct gacggtaatt caatcatcgt tccgactttc ccagtaaagc catactgacg
    16201 caatactgta atagcttgtt ttaattggtc ggcatcattg acaaaaggaa agataacaga
    16261 tagattgggg ttggtttgat aaacttctgt aacgacatgt gcttcagcct gaaattcatc
    16321 caaacacgcc agtaaacgcc tggttcctct atagccaaac aagggatgcc cttcgtcaaa
    16381 aaactcttta gtccccacta aacaattggc ttctgtattc gttaattcag taaaacgata
    16441 ccaaacttct tcatctgagt acagggagca gatagtctct agataatctt ttacaaattg
    16501 ctgacaactt gctaatatga tgttttgatt aagctctctc aacaaatatt ccccacgaat
    16561 catgccgacg tggtgaaata gttgaggata aattttttca agaatttttt cgccactaag
    16621 ggcaagttga tttctcatag ccattcacct cgtcttaaaa gatacaatat catatataat
    16681 tatatcaaaa tttcctctag ttcaaaagct tcataaggtt cagataactc atatcctaaa
    16741 aattctgcta cttttttgga agcctcatta tgagcatccc aaagtggaaa catatctcta
    16801 tttaaactct ctagaatcat tgcagcacca agctttttag caaatccatt tccttgttcg
    16861 tttggtctag ttgcaacttc cacttcaact gctttacggt aaactaaccc tgaggaaatc
    16921 ccagcaatca gttcattatt tttaagaatc acaaagccaa atccaccttt taaaacaaaa
    16981 tcctgatagg actcaaaatc cccctgtaaa tcctgtgacc attcttccgt agaaaagcag
    17041 ttataaatat gattatcaat aggcacaata ttgtaacctt cctctaaatg agtaactaga
    17101 ttatttaaaa attcaacttg aaaatttgcc ttatctttaa aagaatagcg agtaaaagaa
    17161 tttagcccca cttttcgatg cagaaaatct tgccaattag tattttctga aataataatc
    17221 ttataatcta aaccatattt tctaacaaaa tcttcccaaa atctggaatc caactcccct
    17281 gctaggtata gaaaattccc aacatcataa aagcttgttg tcctatcatt gtttcggtag
    17341 acagttccaa tttgagattt aagaccatag agaaccatat ttttcttcca tgttttaaat
    17401 agttccatta tccctctact tcaccttcca attcatgtaa gaagtcttgt ccagttctgg
    17461 aaatcctaat aattcagact taaccttcaa gactaatggc gatgcatttt cttctgtaat
    17521 ctcttgaata tccatccaaa tatatccaag tgaatcattc gcaccatcag acacagcttc
    17581 cgaagtcgta acttgaggtg cactctcatt catttcaaca tcatacaagg ccatgacatg
    17641 gtcaaccata aaatttgtta actcttctct gacgaaaaca tcgtagattc gaggattaga
    17701 gtagcttcta acagtaaatc ccgtctcttc cataacttct ctagtcagcg tttccgtcag
    17761 tccttcacca agttgctgac tgcctccagg tagatcatac cgatgttgat aagggcctct
    17821 cgttttttca atgcagagta acttcccatt ttctaagcaa acagcgtaga ctccaaagtg
    17881 ttttttgatt tccatccaac tcctcctact tcaaagacca gccaccatct attgtcaaga
    17941 tttgtccttg catggcgctc gcttttccac ttgctaaaaa aagactaagc tctgctattt
    18001 cctctggctc aatccagcgc ttgattggag tttcactagc cacccagtca gccaaaccac
    18061 ctggttcaaa atccgcagcg gtcatagctg tcttgactgc tcctggagcg ataccaaaga
    18121 cctgaatccc agcttcagca tagtctagag ccaactgctt ggtgaagcca gccaaggcat
    18181 gcttggatga agtataggcg tgaccacctc cacctgctag gctagaagca atggaacaca
    18241 tattgatgat gattcccttt ttattttcca gcatttgtgt caaataatac cgagtcaact
    18301 ctactggaat aatgtagttg atttcaaaaa tctcttgaat gtcctgcgcc gtttgttcca
    18361 acagtggttt gtaatcatcc aaaactccag cagtattaca caaaacatcc acctgagggc
    18421 accagtcaaa aataggttcc aagtccaagg tcaaatctct ctgtaaaaag tgaaaatcac
    18481 cctctaagag tggcttttca ccttggtcaa ctccataaac ttgatagccc ttctctaaaa
    18541 agaggcgagc ttgagccaat ccgatccctg aactcactcc tgtaatgagt acacgtttag
    18601 tcatgcactt ctacccaatc cgttgccaaa acatcacaaa ctgtcgggct ccacatggaa
    18661 aaaccttctc cttcgccaga aacgttgatt aggaaataag gtgtcatttc aagtgcaagc
    18721 ccattttgct cgatggtatc aaagagttgg acatagtttt ccgcacctcc ccaaccagtt
    18781 cgtacatatt ttctcttagc ctttaaccca ggcaggatct cttcaaatgt catgtttttc
    18841 tcctttaatt ctacattctt catttaatta tagcaaaaaa ccgctttata cggctttttg
    18901 aatgtgagtt attcaaacct gctactactt acggcaaatt attccctgca gcaagataaa
    18961 tttcatacca ttcttttctt gttaagctaa agtttgccgc tcggctaact tctctcaagt
    19021 gcttaggatt tgttgtacct acgactgcct gcatttttgc tggataacgc aatatccaag
    19081 aaatggcaat agttgaagag gttactccat atttaatagc taaacgatca agtacttgat
    19141 ttaaagcttg aaatttctca tttccaacaa aattcccttt aaaatacccg aattgtaaga
    19201 cagaccatgc ttgaatgacc acatcgtgta attggcaata ttcaaaaatg ctgccatctc
    19261 gcatagctgc ttgactatct tccatattaa catgaaaagc tgattcaaat cctggagtaa
    19321 aagccgcact caattgtagc tgattaacag ctaacggctg cttgacatct tttttaagca
    19381 actccatcat cataggattt tgattagaaa ctccaaaatc tcgaacttta ccttgtttat
    19441 aaaggagatt aaaggcttct gctacttggt cagattccat caaagcatct ggtcgatgaa
    19501 ggagcaagct atctagatga tcaatcttca atctttgcaa aataccgtct actgatttta
    19561 taatatagtc cttagaaaaa tcaaaatagg taaattcttc aatgcgaatg ccacatttgg
    19621 actgaatcca catcttttct cttaaatctg gacgattttt taggacaaga cctaacagtt
    19681 cttcacaacg accacgacca taaatatcag ccaagtcgaa ggcattgatt ccaacagaaa
    19741 gtgctgtttc tacaagctct tcaacttctt ttacagactt atcttttatt ctcatcattc
    19801 cgagaacaat ttctgataat tctttgtcat cttgaccaag agttatgtat ctcatcaaat
    19861 ttttctcctt taatttctaa cattcttccc ttcattataa caaaaaaccg ctttgcaacg
    19921 gctttttgac tatacttcac tccattttat cttcttaaac ccacggaaca agacaaagat
    19981 tccaataaag aggacagcta aaggaataac ttttgtaagg aaaacatttg aaattcccat
    20041 ccactcataa taacggagca gagaacccac cacaagatgg gcaataatca tactgacaaa
    20101 tggacgaaag accgcttctt tccaattcca aataccgata actagcgaaa tcgtaaagac
    20161 agacaaacta tcccagggag ccggaatata aaaggctcct tcttgtatga agcttgccat
    20221 tcctacatat cctaaaacaa ctagaagaac tatagtccca acaacaatgt aagtgccaat
    20281 tttcatttta ggagaatctt ggactaaact tcttcgtaaa attgtggcca caagtccaaa
    20341 tccaatcaga aaaataagaa gttgccctaa aaatgtgagc aaattgactg ttaagagagg
    20401 acctttagaa aaatcactta gtagttgata ataacgtaat acagccagga caagaattgg
    20461 cgtcaaaagg gactctttga tagaactgcg aggtgctccc ttgagaatct ctttcattat
    20521 ttttttagga ttcttaccta gataatcctc tgcactcatg ccatctcgtt ctgcttctga
    20581 gaaatctagc atcatcaaat agatctgctc tctgagatag tcttcatcat agagaaatcc
    20641 agcaagatta aaactttccc acaactcctc aaaatacttt tgattctcct cagaaaactc
    20701 atgtagcaaa gcgcttgttt cttcgtaata cttcattttc ttcatggttt aacccccatt
    20761 cttaatccct tctacttttt gactcaaatc gtcccattgc tgccaaaaga ctgagacacg
    20821 ctcttctcct tctttcgtta atgaaaaata cttccgatct ggaccatctg gcgacgggcg
    20881 catgtcgcct cttatccatt gatttttttc taacttttgc aacaaaggat aaatagttcc
    20941 tggaacgata gtatcaaatc cagcctctcg caaagtctga accaactcat aaccataccg
    21001 ctctttttga ccaatcatat ccaagacaca accttcaaga acacctttta atagctgagt
    21061 ttctttcatc acttctccct tctaatctat tttgtaatac ctactagtaa cttcacctat
    21121 agtatattac ttctacacta gtttgtaaag cata
//
LOCUS       DAVBHS010000017        20571 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVBHS010000017 DAVBHS010000000
VERSION     DAVBHS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20571
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.017"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            128..2110
                     /locus_tag="VQ651_000458"
     CDS             128..2110
                     /locus_tag="VQ651_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2753500.1"
                     /translation="MKSSKLLALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQGSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            2411..7714
                     /locus_tag="VQ651_000459"
     CDS             2411..7714
                     /locus_tag="VQ651_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HET2753501.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATANENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLFSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKTPGNSTWYKGNRVVAPEPGSVNRLSITLKSDGQLNASNNDVNLFDTVTLPGAVN
                     DNLKNEKKILLKAGTYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVFLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVNDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGQGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLVSDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(7881..10040)
                     /locus_tag="VQ651_000460"
     CDS             complement(7881..10040)
                     /locus_tag="VQ651_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HET2753502.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGIYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(10037..10633)
                     /gene="recU"
                     /locus_tag="VQ651_000461"
     CDS             complement(10037..10633)
                     /gene="recU"
                     /locus_tag="VQ651_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HET2753503.1"
                     /translation="MINYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            10699..11226
                     /locus_tag="VQ651_000462"
     CDS             10699..11226
                     /locus_tag="VQ651_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HET2753504.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            11296..11625
                     /gene="gpsB"
                     /locus_tag="VQ651_000463"
     CDS             11296..11625
                     /gene="gpsB"
                     /locus_tag="VQ651_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HET2753505.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            11646..12026
                     /gene="rnpB"
                     /locus_tag="VQ651_000464"
     ncRNA           11646..12026
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VQ651_000464"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            12111..13268
                     /locus_tag="VQ651_000465"
     CDS             12111..13268
                     /locus_tag="VQ651_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET2753506.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARLPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERKLELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            13281..14675
                     /gene="mapZ"
                     /locus_tag="VQ651_000466"
     CDS             13281..14675
                     /gene="mapZ"
                     /locus_tag="VQ651_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HET2753507.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTNSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            14751..16175
                     /gene="gndA"
                     /locus_tag="VQ651_000467"
     CDS             14751..16175
                     /gene="gndA"
                     /locus_tag="VQ651_000467"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET2753508.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            16187..16876
                     /locus_tag="VQ651_000468"
     CDS             16187..16876
                     /locus_tag="VQ651_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2753509.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            16974..17930
                     /gene="cbpC"
                     /locus_tag="VQ651_000469"
     CDS             16974..17930
                     /gene="cbpC"
                     /locus_tag="VQ651_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HET2753510.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDDLFDNRPVFEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLRSSGAMA
                     TGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMATGWYQDGSTWYYLNAGN
                     GDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(18048..19280)
                     /locus_tag="VQ651_000470"
     CDS             complement(18048..19280)
                     /locus_tag="VQ651_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2753511.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVHIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFVTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(19323..19910)
                     /locus_tag="VQ651_000471"
     CDS             complement(19323..19910)
                     /locus_tag="VQ651_000471"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753512.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCEIAY
                     ALFAYSKVLEPSMFYNDLHTFHAELKKLLEKVTSNTENLGNLQLSWCEGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVILA
                     CSERDDHGLLMFQGDSGKADLFDFGIGSRGYIGVY"
     gene            complement(19810..20146)
                     /locus_tag="VQ651_000472"
                     /pseudo
     CDS             complement(19810..20146)
                     /locus_tag="VQ651_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
BASE COUNT         6410 a         3718 c         4601 g         5842 t
ORIGIN      
        1 cggttacgat attcatattt tttgcaaaga tgttatttga aaaataattt tcaaaaattc
       61 tgaaaattct gttgacaact ttctgaaaag agtctataat ggagagaaag ttttaaagga
      121 gaaaaggatg aaaagttcaa aactacttgc ccttgcgggc gtgacattat tggcggcgac
      181 tactttagct gcatgctctg gatcaggttc aagcactaaa ggtgagaaga cattctcata
      241 catttatgag acagaccctg ataacctcaa ctatttgaca actgctaagg ctgcgacagc
      301 aaatattacc agtaacgtgg ttgatggttt gctagaaaat gatcgctacg ggaactttgt
      361 gccgtctatg gctgaggatt ggtctgtatc caaggatgga ttgacttaca cttatactat
      421 ccgtaaggat gcaaaatggt atacttctga aggtgaagaa tacgcggcag tcaaagctca
      481 agactttgta acaggactaa aatatgctgc tgataaaaaa tcagatgctc tttaccttgt
      541 tcaagaatca atcaaagggt tggatgccta tgtaaaaggg gaaatcaaag atttctcaca
      601 agtaggaatt aaggctttgg atgatcagac agttcagtac actttgaaca aaccagaaag
      661 cttctggaat tctaagacaa ccatgggtgt gcttgcgcca gttaatgaag agtttttgaa
      721 ctcaaaaggg gatgattttg ccaaagcgac ggatccaagt agtctcttgt ataacggtcc
      781 ttatttgttg aaatccattg tgacaaaatc ctctgttgaa tttgcgaaaa atccgaacta
      841 ctgggataag gacaatgtgc atgttgacaa agttaaattg tcattctggg atggtcaaga
      901 taccagcaaa cctgcagaaa actttaaaga tggtagcctt acagcagctc gtctctatcc
      961 aacaagtgca agtttcgcag agcttgagaa gagtatgaag gacaatattg tctatactca
     1021 acaaggctct attacgtatc tagttggtac aaatattgac cgtcagtcct ataaatatac
     1081 atctaagacc agcgacgaac aaaaggcatc gactaaaaag gctctcttaa acaaggattt
     1141 ccgtcaggct attgcctttg gttttgatcg tacagcctat gcctctcagt tgaatggaca
     1201 aactggagca agcaaaatct tacgtaatat ctttgttcca ccaacatttg ttcaagcaga
     1261 tggtaaaaac tttggcgata tggtcaaaga gaaattggtc acttatgggg atgaatggaa
     1321 ggatgttaat cttgcagatt ctcaggatgg tctttacaat ccagaaaaag ccaaggctga
     1381 atttgctaaa gctaaatcag ccttacaagc agaaggtgtg actttcccaa ttcatttgga
     1441 tatgccagtt gaccagacag caactacaaa agttcagcgc gtccaatcta tgaaacaatc
     1501 cttggaagca actttaggag ctgataatgt cattattgat atccaacaac tacaaaaaga
     1561 cgaagtaaac aatattacat attttgctga aaatgctgct ggcgaagact gggatttatc
     1621 agataatgtc ggttggggtc cagactttgc cgatccatca acctaccttg atattatcaa
     1681 accttctgta ggagaaagta ctaaaacata tttagggttt gactcagggg aagataatgt
     1741 agctgctaaa aaagtaggtc tatatgacta cgaaaaattg gtcactgagg ctggtgatga
     1801 ggctacagat gttgctaaac gctatgataa atacgctgca gcccaagctt ggttgacaga
     1861 tagtgctttg attattccaa ctacatctcg tacagggcgc ccaatcttgt ctaagatggt
     1921 accatttaca ataccatttg cattgtcagg aaataaaggt acaagtgaac cagtcttgta
     1981 taaatacttg gaacttcaag acaaggcagt cactgtagat gaataccaaa aagctcagga
     2041 aaaatggatg aaagaaaaag aagagtctaa taaaaaggct caagaagatc tcgcaaaaca
     2101 tgtgaaataa ctgttgcaaa atataagaaa ggatttagta tttctcttga atgctgaatc
     2161 cttttttaca tttgtaaaga aagattctaa atgtactgac ccccaaaagt tggagcctct
     2221 ttttgtcaga atagagaaaa tttttgttaa ttttacttgt ttcctattgc tttctcagct
     2281 attatttgtt atattaaaag tacaattatt ttttatttat cagggttaag cattgcactt
     2341 tcagaggaag gagtattttt taaaaaggaa atgtaaacgc ttgctaaaaa atgaaaggat
     2401 ttagaagttt atgaataaag gattatttga aaaacgttgt aaatatagta ttcggaaatt
     2461 ttcattaggt gttgcttctg ttatgattgg agctgcattc tttgggacaa gtccggttct
     2521 tgcagatagc gtgcagtctg gttccacggc gaacttacca gctgatttag ctactgctct
     2581 tgcaacagca aacgagaatg atgggcgtga ttttgaagcg cctaaggtgg gagaagacca
     2641 aggttctcca gaagttacag atggacctaa gacagaagaa gaactattag cacttgaaaa
     2701 agaaaaaccg gctgaagaaa aaccaaaaga ggataaacct gcagctgcta aacctgaaac
     2761 acctaagacg gtaactcctg aatggcaaac ggtagagaaa aaagaacaac agggaacagt
     2821 cactatccga gaagaaaaag gtgtccgcta caaccaacta ttctcaactg ctcaaaatga
     2881 taacgcaggc aaaccagccc tgtttgaaaa gaagggcttg accgttgatg ccaatggaaa
     2941 tgcaactgtt gatttaacct tcaaagaaga ttctgaaaag ggcaaatcac gctttggtgt
     3001 cttcctgaaa tttaaagata ccaataataa tgtttttgtc ggttatgaca aggatggctg
     3061 gttctgggaa tacaaaactc caggtaatag cacatggtat aaaggcaacc gtgtagttgc
     3121 accagaaccg ggttctgtaa accgtctttc tatcactctc aagtcagatg gacaactcaa
     3181 tgccagcaat aacgatgtca atctctttga cacagtgaca ctgcctgggg cggtcaatga
     3241 taatcttaaa aatgagaaga agatccttct caaggcagga acttatggca atgaccgtac
     3301 agttgtcagc gttaaaacag acaatcaaga aggcgtaaaa gcggatgata ctcctgccca
     3361 gaaagaaaca ggtccggttg ttgacgatag caaggtgact tatgatacga tccagtctaa
     3421 ggtcctcaaa gcagtgattg accaagcctt ccctcgtgtc aaggaataca gcttgaatgg
     3481 acatactttg ccaggacagg tgcaacagtt caaccaagtc tttatcaata accaccgaat
     3541 cacccctgaa gtcacttata agaaaatcaa tgagacaaca gcagagtact tgatgaagct
     3601 tcgcgatgat gctcacttaa tcaatgcgga aatgacagta cgcttgcaag ttgtggacaa
     3661 tcaattgcac tttgatgtga ccaagattgt caaccacaat caagtcactc caggtcaaaa
     3721 gattgatgac gaaagaaaac tactttcttc tattagtttc ctcggcaatg ctttagtctc
     3781 tgtttctagt gatcaaactg gtgctaagtt tgatggggca accatgtcaa acaatacgca
     3841 tgtcagcgga gatgatcata tcgatgtaac caatccaatg aaagacctag ccaagggtta
     3901 catgtatgga tttgtttcta cagataagct tgctgctggt gtttggagta actctcaaaa
     3961 tagctatggt ggtggttcga atgactggac tcgtttgaca gcttataaag aaacagtcgg
     4021 aaatgccaac tatgtaggaa tccacagctc tgaatggcaa tgggaaaaag cttataaggg
     4081 cattgttttc ccagaataca cgaaggaact tccaagtgct aaggttgtta tcactgaaga
     4141 tgccaatgca gacaagaaag tcgattggca agatggtgcc attgcttatc gtagcattat
     4201 gaacaatcct caaggttggg aaaaagttaa ggatatcaca gcttaccgta tcgcgatgaa
     4261 ctttggttct caagcacaaa atccattcct tatgaccttg gatggtatca agaaaatcaa
     4321 tctccacaca gatggtcttg ggcaaggtgt tttccttaaa ggatatggta gcgaaggcca
     4381 tgactctggt cacttgaact atgctgatat tggtaagcgt atcggtggtg tcgaagactt
     4441 caagacccta attgagaagg ctaagaaata tggagctcat ctaggtatcc acgttaacgc
     4501 ttcagaaact tatcctgagt ctaaatactt caatgaaaaa attctccgta agaatccaga
     4561 tggaagctat agctatggtt ggaactggct agatcaaggt atcaacattg atgctgccta
     4621 tgacctagct catggtcgtt tggcacgttg ggaagatttg aagaaaaaac ttggtgacgg
     4681 tctcgacttt atctatgtgg acgtttgggg taatggtcaa tcaggtgata acggtgcctg
     4741 ggctacccac gttcttgcta aagaaattaa caaacaaggc tggcgctttg cgatcgagtg
     4801 gggccatggt ggtgagtacg actctacctt ccatcactgg gcagctgact tgacctacgg
     4861 tggctacacc aataaaggta tcaacagtgc catcacccgc tttatccgta accaccaaaa
     4921 agatgcttgg gttggggact acagaagtta tggtggtgca gccaactatc cactgctagg
     4981 tggctacagc atgaaagact ttgaaggctg gcagggaaga agtgactaca atggctatgt
     5041 aaccaactta tttgcccatg acgtcatgac taagtacttc caacacttca ctgtaagtaa
     5101 atgggaaaat ggtacaccgg tgactatgac cgataacggt agtacctata aatggactcc
     5161 agaaatgcga gtggaattgg tagatgctga caataataaa gtagttgtaa ctcgtaagtc
     5221 aaatgatgtc aatagtccac aatatcgcga acgtacagta actctcaacg gacgtgtcat
     5281 ccaagatggt tcagcttact tgactccttg gaactgggat gcaaatggta agaaactttc
     5341 tactgataag gaaaagatgt actacttcaa tacgcaggcc ggtgcaacaa cttggaccct
     5401 tccaagcgat tgggcaaaga gcaaggttta cctttacaag ctaactgacc aaggtaagac
     5461 agaagagcaa gaactaactg taaaagatgg taaaattacc ctagatcttc tagcaaatca
     5521 accatacgtt ctctatcgtt caaaacaaac caatcctgaa atgtcatgga gtgaaggcat
     5581 gcacatctat gaccaaggat ttaacagtgg taccttgaaa cattggacca tttcaggcga
     5641 tgcttctaag gcagaaattg tcaagtctca aggggcaaac gatatgcttc gtattcaagg
     5701 aaacaaagaa aaagttagtc tcactcagaa attaactggc ttgaaaccaa ataccaagta
     5761 tgccgtttat gtcggtgtcg ataaccgtag taatgccaag gcaagtatca ctgtgaatac
     5821 tggtgaaaaa gaagtgacta cttataccaa taagtctctc gccctcaact atgtaaaagc
     5881 ctacgcccac aatacacgtc gtaacaatgc tacagttaac gatacaagtt acttccaaaa
     5941 catgtacgcc ttctttacaa caggatcgga cgtctcaaat gttactctga cattgagtcg
     6001 tgaagctggt gatcaagcaa cttactttga tgaaattcgt acctttgaaa acaattcaag
     6061 catgtatgga gacaagcacg atacaggtca aggaaccttc aagcaagact ttgaaaatgt
     6121 agctcaagga atcttcccat ttgtagtggg tggtgtcgaa ggtgtcgaag acaaccgcac
     6181 tcacttgtct gaaaaacacg atccatatac acaacgtggt tggaacggta agaaagtcga
     6241 tgatgttatc gaaggaaatt ggtcactcaa gacaaatgga ctagtgagcc gtcgtaactt
     6301 ggtttaccaa accatcccac aaaacttccg ttttgaagca ggtaagacct accgtgtaac
     6361 ctttgaatac gaagcaggtt cagacaatac ctatgctttt gtagtcggta agggagaatt
     6421 ccagtcaggt cgtcgtggca ctcaagcaag caacttggaa atgcatgaat tgccaaatac
     6481 ttggacagat tctaagaaag ccaagaaggc aaccttcctt gtgacaggtg cagaaacagg
     6541 ggatacttgg gtaggtatct actcaactgg caatgcaagt aatactcgtg gtgattctgg
     6601 tggaaatgcc aacttccgtg gttataacga cttcatgatg gataatcttc aaatcgaaga
     6661 aattacccta acaggtaaga tgttgacaga aaatgctctg aagaactact tgccaacggt
     6721 tgccatgact aactacacca aagagtctat ggatgctttg aaagaggcgg tctttaacct
     6781 tagtcaggcc gatgatgata tcagtgtgga agaagcgcgt gcagagattg ccaagattga
     6841 agccttgaag aatgctttgg ttcagaagaa aacggctttg gtagcagatg actttgcaag
     6901 tcttacagct cctgctcagg ctcaagaagg tcttgcaaat gcctttgatg gaaacttatc
     6961 tagtttatgg catacatctt ggaatggtgg agatgtaggc aagcctgcaa ccatggtctt
     7021 gaaagaacca actgaaatca ctggacttcg ttacgttcca cgtggatcag gttcaaatgg
     7081 taacttgcgt gatgtgaaac ttgttgtgac agatgagtct ggcaaggagc atacctttac
     7141 tgcaactgat tggccagata acaataagcc aaaagacatt gattttggta agacaattaa
     7201 ggctaagaaa attgtcctta caggtactaa gacttacgga gatggtggcg ataaatacca
     7261 atctgcagcg gaactcatct ttactcgtcc acaggtagca gaaacacctc ttgacttgtc
     7321 aggctatgaa gcagctttgg ctaaagctca gaaattaaca gacaaagaca atcaagagga
     7381 agtagctagc gttcaggcaa gcatgaaata tgcgacggat aaccatctct tgacggaaag
     7441 aatggtggaa tactttgcag attatctcaa ccaattaaaa gattctgcta cgaaaccaga
     7501 tgctccaact gtagagaaac ctgagtttaa acttagctct ttagtttccg atcaaggtaa
     7561 gacgccagat tataagcaag aaatagctag accagaaaca cctgaacaaa tcttgccagc
     7621 aacaggtgag agtcaatctg acacagccct cttcctagca agtgttagtc tagccctatc
     7681 tgctctcttt gtagtaaaaa cgaagaaaga ctagtattta gtaaaacctc ttaacaagat
     7741 tacggaagca gtctctatct tttccaatga ggtttatagt acagaaaaag cctgagaaga
     7801 catcttctca ggcttttgtt aagcacataa atacaatagt gctatgacaa aatcacccag
     7861 aaaaatctgg gtgataaatg ttatggttgt gctggttgag gattctgatt ttgttgatca
     7921 ggggttgtat ttgattgttg cgtattattg ttaggattgg tagtcgtact attatttgtg
     7981 cttggagtgg ttgagctaga ctgtgaagtt gaactatctg atgatgagct tgaactttca
     8041 gttgatgggg gttgttgtgg agcaggtgag ctccacgtag aacgagcacc atttttaaat
     8101 acgaattctc catttctgta gagcccctct ggtatattcc aatcctctgg attgcttcct
     8161 tcagacaggt aggtcatcat agagcggtaa actttggcag cgaccgtaag gccattgcct
     8221 acaagtggtg tcagacggtt agaatagcct gtccatacag ccattgaata tttacgcgta
     8281 tagccagcaa atagttcatc aggtgctaca aattgagagg tcttgatgtg gttttcaatt
     8341 tcctcgtctg tatagttaga ggttcctgtt ttaccagcct gagggagcca agcaagatag
     8401 gcatttcgtc cagttccata agtcaagact gttttcatca tgtcggtcat catataggct
     8461 gtcgtttcct tcatggcacg agttccgaca ttagagaact ctttttcact cccatcacta
     8521 aagacgactt tatggatata cattggttta tagtaagttc caccatttgc aaaggcagcg
     8581 taagcagcag ccatcttttc actacttgct ccatattttt tgtctgattc ggttgtgtta
     8641 cttgaaatgg catttgagta gtgaatactt gggtagtcga ttccgagacc atttaggaaa
     8701 gtcttggcgc ggttgagtcc gaccttgttt agagtttcca cggctgggac gtttcgcgat
     8761 tgttgcaggg cgtattgcaa ggtgatgttg ccaaagtagc ccctatccca gttataaaca
     8821 ggggtatttg tcccagggta gttatagggc tcatcgtgaa cgatagtagc agttgaatcg
     8881 tagataccgt actccaaggc aggagcatag tctgtgatcg gtttcatagt tgatccccag
     8941 tcgcggtttg tttctactgc ttggttaatt ccgaaggaaa cattacttga ctgatggcgt
     9001 gctcctagct gggcaatgac tttaccgtta gaaacatcaa caatggtaga agcgacttgc
     9061 aattcatcgt ctggataggc aacgtattcg tctgtattgt aaatatccca cagatgtttt
     9121 tgagcttctt ggtctacatt tgtgtagaca tccatcccag ttgtgagcag gttatagcct
     9181 gtttcttctt caacttgatt gatgacttcc ttgaggtaat tatccatgta agcagggtaa
     9241 ttacttgctg atttgagact ttgtagtcca tcagtaattg gtgtattgac tgctttctca
     9301 tactgttcag cagagatgta gccttgattt ttcatttcag ataagaccaa gtttcggcgg
     9361 tcttgggctg cttctggatg tgaatagggg tcatattggt ttggtgcctg aggcattcca
     9421 gccagcaagg ctaactgagg taaacttaaa ttattgaggt ctttaccata gtagttttga
     9481 gctgctgtct gcattccata gttcccatta gacatgtaga ccttatttat atagtaggtc
     9541 aagatttctt gcttggttgc tttttgttct aactgaatcg ctaaccaagc ttcctgagcc
     9601 ttacgagaaa tagtctggtc ggaagtcgaa gttgaaaagt aagtcaactt aatcaactgt
     9661 tgagtgagag ttgatccacc ttggagggaa ttgctttgca gattgcgcaa gaaagctccc
     9721 aggatacgga tggtatcaat ccccctgtgg tcgaagaagc gatggtcttc gatagaaacg
     9781 attgctttaa ccaaatctgt gggaatatca ttagcttggg cattgacgcg gcgttcagaa
     9841 cccaagtcag caatgagttg atttttattg tcgtagattt tactagaagt tgttgcaact
     9901 agtttactct cggataggct aggagccttg ctaacgtagt agaaaaaaac tcctccgcct
     9961 aagacaatgg ctgcgataac caagcttaag aagctaatgc tcagatactt gattaggcgc
    10021 agaatcgttg gtttgttcat cttgttttac cacctaataa atgttctttg ataacattga
    10081 gataaggaat ttgagggaag gcaccagcct tgatttcata tccatattct cgaatatatt
    10141 caagtggcat tgatttttgt cccttatctt gatgatagaa gcgaatcaaa tcgaatgccg
    10201 gcaataagta ggtttcttgc tgagaagaaa agtgaagaag gacaaagcag attccttgtt
    10261 gggcaaggac ttgttccata tgctgaatct gatgtggatg aaaatttttc atcggaatcg
    10321 cacgtttttg ttttgtttcc ttgacttcaa agtcgatgta atatccatta taaacgccag
    10381 aatagtccgt cgttgaagct tgtcgaaaat aggcttcaac aatcttggca cgacttcgtt
    10441 gtggatagtc cacttgtacg atttgaatag gagttggttt cttatgtata acagccaagc
    10501 cctgagacaa atagtagtcg ttggtagcat tgatcatctt ttcaaaagac attcctcgat
    10561 ttgcgaaatt tttgggttga gaaagagatg tttgtctttt ttgtgatgaa actttatgtg
    10621 gatagttgat cataattctc cttattggta caataacatc actttattat accataattt
    10681 gcacagaaag ggttaaaaat ggctacagct ttggttttag gctattctgc ttttgatttg
    10741 ggtttatttt ctgataagga tcctcgtctt aaattgatta aaaaagcgat acgtaaagat
    10801 ttagaggcta tggcagcaga tggggtgtct tggcttgtgt ttacagggag tttgggtttt
    10861 gaatattggg ttttagaggt tgctcaggag atgaaaaccg aatacggttt ccagttggcc
    10921 accatttttg cttttgaaac ccatggggaa aattggaatg aaggcaatca gatgaaactg
    10981 agtcgcttta agcaggtaga ctttgtcaaa tatgcttatc ctcgctatga acacaagggg
    11041 cagttaagag attaccagca gtttttgctg gaaaacacga ctagttccta tcttttttat
    11101 gatgaagaaa atgaaacaaa actagcttat ttttaccaaa agatgaaaaa tcaagaggac
    11161 tattttataa agagattaac atttgatcaa ttaaatgaac ttgctgaaaa tttttccgaa
    11221 aattgaagct ttgaccttga tttttgtttg ccttttttta tataataata ctagcaagcg
    11281 agaatggaga gagacatggc aagtattatt ttttcagcga aagatatttt tgaacaagag
    11341 tttggacgtg aagtccgtgg ctataataaa gtagaagttg acgagttttt agacgatgtc
    11401 atcaaggact atgaaaccta tgctgccttg gtcaagtcac ttcgtcagga aattgcggat
    11461 ttgaaggaag aattaactcg taaaccgaaa ccttcaccag ttcaagcaga accccttgaa
    11521 gcggcaatta caagttctat gacgaatttt gatattttga aacgcctgaa tagattggaa
    11581 aaagaagttt ttggtaaaca aattttagat aactcagatt tttaagtagt tatttgagat
    11641 gtgcaatttt tggataatcg cgtgaggaga attgcttctc atgaggaaag tccatgctag
    11701 cacaggctgt gatgcctgta gtgtttgtgc taggcgaaac cataagccta gggacgagaa
    11761 atcgttacgg cagttgaaat ggctaagtcc ttggataggc cagagtaggc ttgaaagtgc
    11821 cacagtgacg gagtctttct ggaaacagag agagtggaac gcggtaaacc cctcaagcta
    11881 gcaacccaaa ttttggtcgg ggcatggagt atgcggaaac gaacgtagta ttctgactgc
    11941 tatcagctag agctgttagt ggtagacaga tgattatcga aggaagtggt cctagtcact
    12001 tctggaacaa aacatggctt atagaaaatt gcatataggt tggggctgag aaattttctc
    12061 aacctcattt tttaaagtgg acatatagaa aggtcttgca agactgtaac atgaaaaaag
    12121 aatttaattt aattgcaact gtggcagcag ggcttgaggc tgtcgttggt cgtgaagtgc
    12181 gagagttggg ctacgattgt caggttgaaa atggacgtgt tcgttttcaa ggagacgtga
    12241 gagctattat cgaaaccaac ctttggcttc gggcagcaga tcgtatcaaa attatcgtag
    12301 gaacgttccc agctaagact tttgaagagc tatttcaggg agttttcgct ttagattggg
    12361 aaaattattt accacttgga gctcggttac cgatttcaaa agctaaatgt gttaagtcca
    12421 aacttcacaa tgagcccagt gttcaggcta tttctaagaa agctgttgtc aagaaattgc
    12481 agaaacacta tgctcgccca gaaggggttc ctctgatgga gaatggccca gagtttaaga
    12541 ttgaggtctc tattctcaaa gatgtggcaa ctgtcatgat tgatacgacc gggtctagcc
    12601 tctttaaacg tggttatcgt accgaaaaag gtggcgctcc tatcaaggaa aatatggcag
    12661 cagccatttt acaactttct aactggtatc cagacaagcc tttgattgat ccgacctgtg
    12721 gttcggggac tttctgtatt gaggcagtta tgattgctag aaagatggcg ccaggtcttc
    12781 gtcgctcttt tgcatttgag gaatggaact ggatcagcga tcgcttgatt caagaagtgc
    12841 gcacagaagc ggctaaaaaa gtagaacgta agcttgagtt ggatatcatg ggctgtgata
    12901 ttgatgctcg catggtggaa attgctaagg ccaatgctca ggcagctggt gttgcaggag
    12961 atattacttt taagcagatg cgcgtgcagg atttacgttc cgataaaatc aatggagtaa
    13021 tcatttccaa tccgccttat ggtgaacgtt tgtcagatga tgcaggagtg accaagctct
    13081 atgctgagat ggggcaagta tttgcaccgc tgaaaacttg gagcaaattt atcctgacta
    13141 gtgatgaagc ttttgaaagc aagtatggta gccaagcaga taagaagcgt aagttataca
    13201 acggaacctt gaaagtggat ctatatcaat attttggtca gcgtgtcaaa cggcaagagg
    13261 taaaatagaa agggatactc atgagtaaaa aaagacgaaa tcgtcataaa aaagaaggtc
    13321 aagaaccgca atttgatttt gatgaagcaa aagagctaac agttggtcaa gctattcgta
    13381 aaaatgaaga agtggaagca ggagtcttgc ctgaggattc cattttggac aagtatgtta
    13441 agcaacacag agatgaaatt gaggcggata agtttgcgac tcgtcaatac aaaaaagagg
    13501 agttcgttga aactcagagt ctggatgatt taattcaaga gatgcgtgag gctgtagaga
    13561 agtcagaagc ttcttcggag gaagttccat cttctgaaga catcttacta cccttgcctc
    13621 tggacgatga ggagcaaggc ttggatcctc tattgctaga tgatgaaaat ccaacagaaa
    13681 tgactgaaga agtggaagag gagcaaaacc tttctcgtct ggatcaagag gactcagaaa
    13741 agaaaagtaa aaaaggcttt attttgaccg ttttggcgct tgtatcagta attatttgtg
    13801 tcagtgctta ttatgtctat cgtcaagtgg ctcgttcgac caaggaaatt gaaacttctc
    13861 aatcaactac agccaatcaa tcggatgtgg atgattttaa tacactttat gacgcctttt
    13921 acacaaatag caataaaacg gctttgaaaa atagccagtt tgataaactg agtcaactca
    13981 agactttact tgataagctg gaaggtagtc gtgaacatac gcttgccaaa tctaaatatg
    14041 atagtctagt aacgcaaatc aaggccattc aagatgtcaa tgcacaattt gaaaaaccag
    14101 ctattgtgga tggtgtgttg gataccaatg ccaaagccaa atcggatgct aaatttacgg
    14161 atattaaaac tggaaatacg gagcttgata aagtgctaga taaggctatc agtcttggta
    14221 agagccagca aacaagtact tctagctcaa gttcaagtca aactagcagc tcaagttcaa
    14281 gtcaagcaag ttcaaatacg actagtgagc caaaaccaag tagttcaaat gagactagaa
    14341 gtagtcgcag tgaagtcaat atgggtctct cgagtgcagg ggttgctgtt caaagaagtg
    14401 ccagtcgtgt tgcctataat cagtctgcta ttgatgatag taataactct gcctgggatt
    14461 ttgcggatgg tgttttggaa caaattctag caacttcacg ttcacgtggc tatatcactg
    14521 gagaccaata tatccttgaa cgtgtcaata tcgttaacgg caatggttat tacaacctct
    14581 ataagccaga tggaacctat ctctttaccc ttaactgtaa gacaggctac tttgtcggaa
    14641 atggcgctgg tcatgcggat gacttggact actaagcagt cgttacaaaa ttctttcttt
    14701 tcaaaagtaa aaatgataaa ataaaacaaa tgaaacaaga ggagtgtcaa atgacaaaag
    14761 ctaactttgg tgtcgtaggt atggccgtaa tgggtcgtaa ccttgccctt aatattgaat
    14821 cacgtggtta cacagttgct atctacaacc gtagtaaaga aaaaacggaa gatgtgattg
    14881 cttgccatcc tgaaaagaat tttgtaccaa gctatgacgt tgaaagtttt gtaaactcaa
    14941 tcgaaaaacc tcgtcgtatc atgctgatgg ttcaagctgg acctggtaca gatgctacta
    15001 tccaagccct tcttccacac cttgacaaag gtgatatctt gattgacgga ggaaacactt
    15061 tctacaaaga taccatccgt cgtaatgaag aattggcaaa ctcaggtatc aactttatcg
    15121 gtactggagt ttctggtggt gaaaaaggtg cccttgaagg tccttctatc atgcctggtg
    15181 gacaaaaaga ggcctacgaa ttggtttcgg atgttcttga agaaatctca gctaaagcac
    15241 cagaagatgg caaaccatgt gtgacttata tcggtcctga tggagctggt cactatgtga
    15301 aaatggttca caacggtatc gagtacggtg atatgcaatt gatcgcagaa agctatgact
    15361 tgatgcaaca cttgctaggc ctttctgcag aggatatggc tgaaatcttt actgagtgga
    15421 acaagggtga attggatagc tacttgatcg aaatcacagc tgatatcttg agccgtaaag
    15481 acgatgaaga ccaagatgga ccaatcgtag actacatcct tgatgctgca ggtaacaagg
    15541 gaactggtaa atggacgagc caatcatctc ttgaccttgg tgtaccattg tcactgatta
    15601 ctgagtcagt gtttgcacgc tacatttcaa cttacaaaga agaacgtgta catgctagca
    15661 aggtgcttcc aaaaccagct gccttcaact ttgaaggaga caaggctgaa ttgattgaaa
    15721 aaatccgtca agccctttac ttctcaaaaa tcatttcata cgcacaagga tttgctcaat
    15781 tgcgtgtagc ctctaaagaa aacaactgga acttgccatt tgcagatatc gcatctatct
    15841 ggcgtgatgg ctgtatcatc cgttctcgtt tcttgcaaaa gattacagat gcttacaacc
    15901 gcgatgcaga tcttgccaac cttcttttgg acgagtactt cttggatgtt actgctaagt
    15961 atcaacaagc agtgcgtgat atcgtagctc ttgcggttca agctggtgtg ccagtgccaa
    16021 ctttctcagc agctattact tactttgata gctaccgttc agctgacctt ccggctaact
    16081 tgatccaagc acaacgtgac tactttggtg ctcacactta ccaacgtaaa gacaaagaag
    16141 gaaccttcca ctactcttgg tatgacgaaa aataagtagg tcagccatgg ggaaacggat
    16201 tttattactt gagaaagaac gaaatctagc tcatttttta agtttggaac tccagaaaga
    16261 gcagtatcgg gttgatctgg tagaggaggg gcaaaaagcc ctttccatgg ctcttcagac
    16321 agactatgat ttgattttat tgaacgttaa tctgggagat atgatggctc aggattttgc
    16381 agaaaaattg agtcgaacta aacctgcctc agtcatcatg attttagatc attgggaaga
    16441 cttgcaagaa gagctggaag ttgttcagcg ttttgcagtt tcatacatct ataagccagt
    16501 ctttatcgaa aatctggtag cgcgtatttc ggcgatcttc cgaggtcggg acttcattga
    16561 tcaacactgc agtctgatga aagttccaag gacctaccgc aatcttagga tagatgttga
    16621 acatcacacg gtttatcgtg gtgaagagat gattgctctg acccgccgtg agtatgacct
    16681 tttggcgaca cttatgggaa gcaagaaagt attgactcgt gagcaattgt tggaaagtgt
    16741 ttggaagtat gaaagtgcga ccgagacaaa tatcgtagat gtctatatcc gctatctacg
    16801 gagcaagctt gatgttaaag gacaaaaaag ctacattaaa accgtgcgtg gtgttggtta
    16861 caccatgcaa gaatagaaaa gcagttgcag ttgtgtaact gcttttttga ggagttttta
    16921 tatattgaca tgcagtcgtg tctttgctac aatcgtttta ggaggatata taaatgaaac
    16981 ttttgaaaaa aatgatgcaa gttctactag cagtcttttt ctttggtttg ctagctacaa
    17041 atacggtatt tgcgaatacc acaggtggcc gatttgttga taaggataat agaaaatatt
    17101 atgtaaaaga tgatcataaa gcaatctatt ggcataaaat agacggtaaa acttactatt
    17161 ttggtgatat tggagagatg gttgtcggtt ggcaatactt agaaattcct ggaacaggtt
    17221 atcgtgatga tttatttgat aatcgtccag tcttcgaaat tggccttcag gagaagtggt
    17281 actattttgg acaagatggt gctttgctag aacaaacaga taaacaagta ctagaggcaa
    17341 aaacgtctga aaatacagga aaagtatacg gtgaacaata tcctctatct gctgaaaaga
    17401 gaacttatta ttttgataat aattatgctg taaagacagg ctggatttat gaagacggca
    17461 attggtatta tttaaataag ctaggaaatt ttggtgatga ttcttacaat ccactaccaa
    17521 ttggtgaagt tgctaagggt tggactcaag attttcatgt tactattgac attgatagaa
    17581 gcaaacctgc tccatggtac tacctccgtt cctcaggagc aatggccact ggctggtatc
    17641 aggaaggtac cacttggtat tatttagacc agccaaatgg cgatatgaaa acaggttggc
    17701 aaaaccttgg gaacaaatgg tactatctcc gttcatcagg agctatggca actggttggt
    17761 atcaagatgg ttcaacttgg tactacctaa atgcaggtaa tggagacatg aagacaggtt
    17821 ggttccaggt caatggcaac tggtactatg cttatagctc aggtgctttg gcagtgaata
    17881 cgaccgtaga tggctattct gtcaactata atggcgaatg ggttcggtaa tgacagggcg
    17941 cttataatta tattagaaca ctcgcttaat caaagaagtg actttgttgt acttgacctg
    18001 acgattgggg agattaaaag catgtgaaat ctacacatgc tttttcgtta gttatgagtg
    18061 ttgaacttga ctaaccattg acaaaagata agagcaatca aaataagata caaaaatatc
    18121 aatgctaact gcaccgaaac gcccgatgct atggataggg ctaataggga gagcagggac
    18181 ggggcgagaa tgtctactgt gctcagagca gagcccaccg tccccatctt gtcactgggg
    18241 atatgggcaa agacagctgc ttgtaggcga gggctgagaa tgccgataat cgtagaactg
    18301 atgaaactgg tcacgaaaat tacgatatag gcttgacaaa ggatactagt tattagagcc
    18361 aatagggaaa tctcacagaa aaccaagagg tgcgtgaaac ggatattttt aaatagatta
    18421 ctgctggtca tattgccaag gatagctcca caagacatga tgatggagag cagggcgaag
    18481 agttgcccaa tgctcagttg tgctagtgtc tgttggatga agcggagggc aatcagccga
    18541 ggggacacat ccaagatggc aatcatactg gtcgtcagaa acagtagtat gacggtatct
    18601 tttaacctca ggagcacagc cagcgactgt ttaagatgtt gaaaatattt cttaaaactc
    18661 agtgctgtat gtgacatttc aatccttttt tcaacctcat acaaggtatg tcgaataaca
    18721 tacaggccca aaaaggcaat gacaaaagtc agagtgttga taagggaaat agtttgaata
    18781 cttataacat tgataaggaa tgcgccagcc agattggcga caatgtggac aaccctcatc
    18841 agagactgcc tgaaccccat ggcatcatgc aggtcatcct taattacact gatgtagagg
    18901 gcgttcatca tgtaggcaga caggtagctc aaggtgtcag agatgaggtt gatgatgaca
    18961 atcataatga ctgaaactaa tgaaaagggc gacaagacga gaaatactgt caaaatagcg
    19021 tagagcagga ttttgatttt ggcaatacag agtgcgtgtt tgactctatt ttcttgaaaa
    19081 tctcctagaa tcccgagaaa gagttgcgat agtaggggaa ccatctctga aatggaaatc
    19141 agtaaaatcg ctagcggagc gaaattgtac tgagccacat aattgataat ggcgaggtag
    19201 aaaatactgt cgccgaatga tgaaaaacag ctgtttagca gcaactgtct atatatgttg
    19261 tttgtccaaa ataatttcat acatttctcc ttatgtttgc acatcgaatg ggaatttatt
    19321 atttaataga caccaatata ccccctgctt cctattccga agtcaaacaa atctgcttta
    19381 ccactatctc cttgaaacat cagtaaaccg tgatcatctc gttcagaaca tgctaaaatt
    19441 acctgttgga tttttttcat cagtaatttg ttttgattgt agacagtggt ttgtagtaag
    19501 ctagttattc cgtggcaata tcctgtcatc atttttagat gatggttaaa aacaaattct
    19561 tgatatttac taataatatc tttgtttccg tcacaatcgt acatacaaag atataagatt
    19621 attccggaaa ttccttcgca ccaagaaagt tgtaaatttc ctaaattttc agtattagaa
    19681 gtaacttttt ctaataattt ttttaattca gcatggaatg tatggagatc attatagaac
    19741 atagaaggtt ctaagacttt agaataggca aataaagcat atgcaatttc acaatatcca
    19801 tgagcaaagc tatagtctaa aatgctttct ttggctgtat ctctttgtag gaaataatga
    19861 taaatttccc catatactat tgtggatgaa agttttaaga gtattgtcat tggtaaattg
    19921 atagtatttt attaatgata ataggacacc agattttccc aatgcaaagt ctatttcttc
    19981 tagagtatca taattctcta ttaatttctt agcggtgacg tttgctaatt gtagatagta
    20041 ttgattttta ctgatagaaa ataaatgtag gagtaaccat agatagcctg catcaccaaa
    20101 tagcagtgag tattgatatt catatgtgtg tcgttctaat tgtcactgcc ttcttgatga
    20161 tagagagtat tttagaatcc agttctgggg ggtacaattt atttagagga aacagtagtc
    20221 cagctattcc gtgttggaat gataaattac ttacgaaatt tccaaattct gttgaagaag
    20281 tgatccttcc ttcagaattt acagttttat ctagtaaaaa tcgtagaaca ctattacatt
    20341 ctgttttgta atcatattta tgttcctcta tttttttaat tttgactaaa ttatttgttt
    20401 gacttaaatt tagtagacat tgtaaccaaa atagttgctc atctgtgagc tgaccttgag
    20461 tatgtgatag ttctagtaat tgaataggat cctttagtta gattcaatca atctataaca
    20521 caacaacata ataatacttt taggataact tgtcaaatat tatagtaaaa t
//
LOCUS       DAVBHS010000018        20406 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVBHS010000018 DAVBHS010000000
VERSION     DAVBHS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20406
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.018"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(1..1680)
                     /locus_tag="VQ651_000473"
     CDS             complement(1..1680)
                     /locus_tag="VQ651_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET2753513.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(1682..1915)
                     /locus_tag="VQ651_000474"
     CDS             complement(1682..1915)
                     /locus_tag="VQ651_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HET2753514.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            2297..2518
                     /locus_tag="VQ651_000475"
     CDS             2297..2518
                     /locus_tag="VQ651_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753515.1"
                     /translation="MTNQVLEENYLCDSLLAFSPPRDLCRLVCILLWIKCLDALLMLG
                     FFVWGCRDSRFTTRKFHPIYQDEVVFAFF"
     gene            complement(2731..2883)
                     /gene="cibB"
                     /locus_tag="VQ651_000476"
     CDS             complement(2731..2883)
                     /gene="cibB"
                     /locus_tag="VQ651_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HET2753516.1"
                     /translation="MMKDLNNCREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(2885..3070)
                     /gene="cibA"
                     /locus_tag="VQ651_000477"
     CDS             complement(2885..3070)
                     /gene="cibA"
                     /locus_tag="VQ651_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HET2753517.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            3248..3931
                     /gene="tsaB"
                     /locus_tag="VQ651_000478"
     CDS             3248..3931
                     /gene="tsaB"
                     /locus_tag="VQ651_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET2753518.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMESLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLTHTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMPEAHLSFEEVLEKVKGASQVTFVGEVGPF
                     VEQIQEHLPRTNYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            3928..4365
                     /gene="rimI"
                     /locus_tag="VQ651_000479"
     CDS             3928..4365
                     /gene="rimI"
                     /locus_tag="VQ651_000479"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET2753519.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            4355..5365
                     /gene="tsaD"
                     /locus_tag="VQ651_000480"
     CDS             4355..5365
                     /gene="tsaD"
                     /locus_tag="VQ651_000480"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET2753520.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(5411..6711)
                     /locus_tag="VQ651_000481"
                     /pseudo
     CDS             complement(5411..6711)
                     /locus_tag="VQ651_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(6987..7338)
                     /locus_tag="VQ651_000482"
                     /pseudo
     CDS             complement(6987..7338)
                     /locus_tag="VQ651_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7445..7737)
                     /locus_tag="VQ651_000483"
                     /pseudo
     CDS             complement(7445..7737)
                     /locus_tag="VQ651_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            7980..9186
                     /locus_tag="VQ651_000484"
                     /pseudo
     CDS             7980..9186
                     /locus_tag="VQ651_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            9179..9827
                     /locus_tag="VQ651_000485"
                     /pseudo
     CDS             9179..9827
                     /locus_tag="VQ651_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            9837..11453
                     /locus_tag="VQ651_000486"
     CDS             9837..11453
                     /locus_tag="VQ651_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2753521.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLSGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
     gene            11473..12317
                     /locus_tag="VQ651_000487"
                     /pseudo
     CDS             11473..12317
                     /locus_tag="VQ651_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813546.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12385..13886
                     /locus_tag="VQ651_000488"
                     /pseudo
     CDS             12385..13886
                     /locus_tag="VQ651_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
     gene            13898..14647
                     /locus_tag="VQ651_000489"
     CDS             13898..14647
                     /locus_tag="VQ651_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET2753522.1"
                     /translation="MSDNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSKLKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            14660..15832
                     /locus_tag="VQ651_000490"
     CDS             14660..15832
                     /locus_tag="VQ651_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET2753523.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGTLP
                     SKNEELMKFFCENNLNITFFDTFSEIKNNIDYYIIALPTDYDEKIGSFNTYEIEQTVS
                     KILRVKPNGKIILKSTVPIGFSNKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVV
                     GDETVEGRKIAELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELD
                     SFAERRSLNAEVVIKGVCLDPRIGNFYNNPSFGFGGYCLPKDTRQLKKEFIEINAPVI
                     EAIDISNTNRKQFIVKQILERKPKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIK
                     ILVYEPNLNVSIDNVIFEKNFELFIKQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
     gene            16227..17090
                     /locus_tag="VQ651_000491"
     CDS             16227..17090
                     /locus_tag="VQ651_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET2753524.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACAGLTASQLSKFELGQSM
                     LSADKLILAIQGINMNFDEFGHKLNNYQESPHMRIGRRVVDCFSHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKNALHSLDKSYPLTEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRWYMKSGYLNIISELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVLWQFLKKMQIFLQTKGSNQKEIEHFIQSLQVLENPQLTALFE
                     LRLQQYKELID"
     gene            17376..17609
                     /locus_tag="VQ651_000492"
     CDS             17376..17609
                     /locus_tag="VQ651_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753525.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            17631..18308
                     /locus_tag="VQ651_000493"
     CDS             17631..18308
                     /locus_tag="VQ651_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2753526.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATSLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            18320..18985
                     /locus_tag="VQ651_000494"
     CDS             18320..18985
                     /locus_tag="VQ651_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2753527.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFFVYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            19057..20283
                     /locus_tag="VQ651_000495"
     CDS             19057..20283
                     /locus_tag="VQ651_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2753528.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLATLEL
                     SALILGSLMSGYLQHTISIKIALYASLLLQLLLLVGFATAHFGLILIFSFLDAFFAGI
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALGTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
BASE COUNT         6377 a         3197 c         4125 g         6707 t
ORIGIN      
        1 ttattcttca tctggtgtga ggatcatcgg gatgatgatc ggttcacgtt cggtattttc
       61 atagaggaag gggcgaatag cgttgacaat ggcaccattg acagattgca cgctagcatc
      121 cttatttttc agtgcgatac gaatggcatt gaagaggata cgctggcttt ggcgaatcaa
      181 gtctccagac tctctcatgt agacaaagcc tcggctgagg atatctgggc cagacagaat
      241 catctgcgat ttgaagtcaa cagttgcgac tgccagaacg acaccgtctt cagatagatc
      301 gcgacgatct ttgaggacag ctgcgccaat ttcaccgata cgatttccat cgacatagat
      361 atcttgggcg ttgaaatgac ctgcgatacg agctgagtca gcagtaaggg caagcacatc
      421 gccattgctc atgataaaga tattgtcttt ctcaacacca gtatccactg ctagtccagc
      481 gtggactttt tgcatgcggt attcaccgtg gacaggcatg aagtattttg gcttaatcaa
      541 gcggagcatg agtttttgct cttgctgacc accgtgtcca gatgtatgga tattgttcac
      601 tttaccgtgg ataacttcga caccagcttc agaaatgatg ttaatcagct tgttgacgct
      661 agtagtgttt ccagggatgg gactagaaga gaagataacg gtatcacctg gttgtaattg
      721 tacttgacgg tgggttccgt tggcgatacg agagagggct gccataggct caccctgact
      781 acctgtacag aggataagaa cttctcctgc aggataatct ttgatttcat ttggctcgat
      841 aaaggttccc ttaggagctt tgatgtagcc aagatcgatt ccgttgacaa tggccttttc
      901 catagaacga ccaaagaccg caatcttgcg tccagtctta acagcagctt ctgttgcctg
      961 ctggagacgg aagatatttg aggcaaagga tgcaaagatg atacgtcctt caataccttg
     1021 gataatcttc ataatggact gaccaacgac tttttcagag ttggtaaagg ttggtacttc
     1081 cgcatttgtc gagtcagaca ggagacagag cacgccttct tcaccaagcg cagccatacg
     1141 atgcaagtcc gcaggttctc caactggagt aaagtcgaac ttaaagtcac ccgtacagac
     1201 gattttccct tgaggagtat gaatgacaat ccccaaaggc tctggaatag agtgagtcgt
     1261 tctaaagaaa gttgccttga gatttttaaa ggtcaactcg gtgttgtggt tgatttcgta
     1321 aagtttggcg ttgcgcaaga ggccgtgttc ttcgagtttc ccacggatca aagccaaggc
     1381 aagcggtcca gcgtagatag ggacatttgc ttgcttgagt aggaacggaa tcccaccaat
     1441 gtggtcctcg tgtccgtgtg tgattaaaac agccttgacg cggtcgatat tgtctacgat
     1501 gtaagagtag tcaggaatga catagtcgat accaagcaag tcatcttctg ggaatttaat
     1561 cccagcatcg acgatgataa tctcgtcttg gtattcaatt ccgtaagtgt ttttcccgat
     1621 ttctcctaga ccaccgatgg caaaaacacc gacttcttca ggtttaagag tgtaggccat
     1681 attagaactc cgtaatttcg aaggcgccag tttctttttc gtaatcgagc aatttgtcag
     1741 acaagagttc gatatattcg atattgtact ctgggcgatt ttcttcgaca agttggcgag
     1801 cagtgatacg gccctcaagt tctgagctgg catcgatgtc taggtaaagc gcgcgtgttg
     1861 tttcacggcg tgggctacgt tcttttgttt cttgataaaa aactttgtaa atcatatagt
     1921 tcctttctat ttacaggtca gcttattcca aacttttagc agatatttgc ttgatttcat
     1981 tccattttgt gtctagattg accttgattc gttacaatta tttcaattat accacaaaat
     2041 gaaaaattag taaaagacgg aaacgagaaa gttaggggac gatttcttcg gtaagcgttt
     2101 gcaaaaacaa agaaaatgtg ttatgttgat aacggaagtg aagtttatta cttgtcataa
     2161 ttccatatag attttaagtc aaggaggttg ccctatgtat aacttatttg cttttgctat
     2221 cggagttcgt ttgctagctt ttatcgtttt tgttttgcgt ttatggagga aatcttctat
     2281 ttgccaaact ggaacgatga caaaccaagt tcttgaggaa aattaccttt gtgactctct
     2341 actcgctttt tctcctcctc gtgacctatg tcgtttggtt tgtattttac tttggattaa
     2401 atgcttagat gccctgctta tgctggggtt ttttgtttgg ggatgtagag atagtagatt
     2461 tacgacacga aaattccacc ccatctatca agatgaggtg gtctttgcat ttttctaaaa
     2521 tgatatgaca atggaatttt caaagcttat tatttttatg ggagtcattg ttctgctcaa
     2581 aaatagagtt gaagcataaa aataaaacaa tacaaacaga taaatcgaat ggtaaaggga
     2641 tggtagtaat gttcaaatca ttgagaatgc tgagcaaact gtaagagatg aagacaataa
     2701 aatttatcct tctaaaaatg tgtttcttat ctagcatgac ttaccaaact ttttacgaag
     2761 gtcttttcca atggtataac ctgccataaa taaagcgata ccaacaccta caccaaagcc
     2821 acccttgatt tcttgcaatt ccttattaga aatttcacga cagttgttca aatctttcat
     2881 catattaatc tcctcctaaa aaataaccag ctgccaaacc agaacctata agtgctgttc
     2941 cacctgcaat agctttccaa gatactgcta ctccaaagat aaccaaggga gcgagaccgc
     3001 catcaatatc tgataattca ttttcagata aggataaaaa ttgattgtca agaatgtcaa
     3061 aatttgtcat tgaactttcc tccttttttc ttgttcactt ttatattcgg aaaagaaaag
     3121 gaaaagtgag cattttttta aattattttt agttaagtga gggcactctt gtctgggggc
     3181 aaagaaaatt cccatgtgcc atcttttgta gtataatagt aagcagacaa aaagatagga
     3241 atgtgttatg aaagtattag cttttgatac gtccagcaag gctctttctc tcgctatttt
     3301 agaggataag caggttcttg ccgagacgac gattaatatc aagaaaaatc acagtattac
     3361 cctcatgcca gccattgatt ttttgatgga gagtttggac tggacgccca aggatttgga
     3421 ccgaatcgtg gtggcggaag ggccaggtag ctacacaggc ttgcgaatcg cggtagccac
     3481 tgccaagacc ttgactcata ctctgaacat tgagttagtt ggtatgtcta gcctcttggc
     3541 tctggtgcca tatcaacaag aaggcttgtt cgttcctttg atggatgcgc gtcgcaacaa
     3601 tgtttatgca ggattttatg aaaatgctaa acctgtcatg ccagaagcgc acctatcttt
     3661 tgaagaggtg ctagaaaaag tcaagggtgc tagtcaggta acctttgtcg gagaagttgg
     3721 cccctttgtg gagcagattc aagaacactt gccaaggact aattataaag aaaccttgcc
     3781 taatgcagcc aatcttgctc ttttagcttg ggacaaggaa gcagactcct tgcatgattt
     3841 tgtgccaaat tacctcaaac gagtcgaggc tgaggaaaac tggctcaaga accataccga
     3901 gtctggtgag tcctacatta aacgcctatg atagaaatca agcgaattca acaacagcct
     3961 gacctagctc aagccatcta cgctgttatg gcagctgttt acctagtcag tccttggact
     4021 ctggagcaaa tccaagcaga tctgtcccaa gaccagactt ggtatgcatt ggcttatgat
     4081 ggggcagaag tgattggatt tctagctgtg caggagaatc tttttgaagc agaagtcctg
     4141 caaatcgctg tcaaaggagc ttatcagggt cagggaattg cgtcagcctt gtttgctcaa
     4201 ttgccgacag ataaggagat tttcctcgaa gtcagaaagt caaatcaacg agcgcaagca
     4261 ttttacaaga aagaaaaaat ggcggtcatc gctgagcgaa aggcctacta ccatgaccca
     4321 gtcgaggacg ccattatcat gaagagagaa atagatgaag gatagatata ttttagcatt
     4381 tgagacatcc tgtgatgaga ccagtgtcgc cgtcttgaaa aacgacgatg agctcttgtc
     4441 caatgtcatt gctagtcaaa ttgagagtca caaacgtttt ggtggcgtag tgcccgaagt
     4501 agccagtcgt caccatgtcg aggtcattac agcctgtatc gaggaggcat tggcagaagc
     4561 agggattacc gaagaggacg tgacagctgt tgcggttacc tacggaccag gcttggtcgg
     4621 agccttgcta gttggtttgt cagctgccaa ggcctttgct tgggctcacg gacttccact
     4681 gattcctgtt aatcacatgg ctgggcacct catggcagct cagagtgtgg agcctttgga
     4741 gtttcccttg ctagccctct tggtcagcgg cggacacaca gagttggttt atgtttcgga
     4801 ggcaggagat tataagattg ttggggaaac ccgtgatgat gcggttggtg aggcttatga
     4861 taaggtcggc cgtgtcatgg gcttgaccta tcctgcaggt cgtgagattg acgagctggc
     4921 tcatcagggg caggatattt atgatttccc ccgtgccatg attaaggaag ataatctgga
     4981 gttctccttc tcaggtttga aatctgcctt tatcaatctt tatcacaatg ccgagcaaaa
     5041 gggagaaagc ctgtctacag aagatttgtg tgcttccttc caagcagcag ttatggacat
     5101 tctcatggca aaaaccaaga aggctttgga gaaatatcct gttaaaaccc tggttgtggc
     5161 aggtggtgtg gcagccaata aaggtctcag agaacgccta gcagccgaaa tcacagatgt
     5221 caaggttatc atcccccctc tgcgactctg cggagacaat gcaggtatga ttgcctatgc
     5281 cagcgtcagc gagtggaaca aagaaaactt cgcaggctgg gacctcaatg ccaaaccaag
     5341 tcttgccttt gataccatgg aataaagagt ggctctttgt caagtgtagt gggtagtcga
     5401 aaagctgcaa tctggagagt acgaaattcg tactctcttt tttgatgttc aaagtcatca
     5461 aaatccgttt tttaaaattg ttaaagttcc ggaaaccaaa ggctttgcgc ttgataatct
     5521 taatcaaatt attggtcgct tcaagtttag cgttggaata aggcagctta agtgcgttga
     5581 taatcttatc tctatccctt aaaaaagtcc aaaagaccgt ttgaaaaagc ggatgaaccg
     5641 ttggcagttc ttgctcaatc aatccaaaga aatggtcggc attcttctct tgaaaatgaa
     5701 agagcaggag ttgatagagt tcgtagtgaa cctgtaatcc ctcagagtaa gaaagcagct
     5761 tatctcgtac ttcccgatgg gttaagtgca ttcgaaacgt gggctgatag aatcgtttct
     5821 gacagagctt gaagctatac tggtgtagta aatcagtcca gtggactgat ttagcccgag
     5881 cctagaaacg aaaaagcgag ggttccaaaa gcgcttgagc gcccgatact ccaaggattt
     5941 tcggtcaaat tggttcatga tttggattcg tactcggttc atagctcggc tcagatgttg
     6001 gacaatgtgg aagcggtcaa gaacaatctt cgcctttgga aagagttgct tagcgagccg
     6061 ataataaggg ctgtacatgt ccatggtgat gacctcgacc agctcccgaa cctctctctg
     6121 atagcgttgg aaatggtttc gaatcaccgc atgagttcgc ccgtctaaaa ttgcgaggat
     6181 ggatttggac tcaaaatctt gggcaatgaa gctcattttg ctctttttga agctatactc
     6241 atcccaactc ataacttttg gcaacttggt ccaatcggtt tcaaacttaa attccctcag
     6301 ttttcggatg acggtggaag ttgagaccgc caatctgtgg gcaatctcag tcatagactg
     6361 cttttcaagg aggagctgag cgattttttg tctcaccatg ttggaaatct ggcaattttt
     6421 cttgacaatg gatgtttgag aaacgaccac cttaaggcaa ttcttgcact gaaagcggcg
     6481 ctttttgaga tgcagtaccg tgggtaaacc ctgacagtcg agaagcggaa ttttagaggc
     6541 tttctggaag tcgtatttga tcatcttccc ttgacaatga ggacaaggag gagcggggga
     6601 atccaacttt gcctgaacga ctagatgggt ttggtatttc agaacagaca agattttgat
     6661 atttttgtct tccaatccga gcaaatctgt ggtattctta agttgttcca tatggaccct
     6721 ttctaatgag ttggttagac gcttttcatt ataggtcata tggggctttt tttctacaag
     6781 aaacgaccct ataattcctg gggtgggatt acccactaca gaaattatag agccaaagca
     6841 ttccaaagtc ttgtctgact ctggaatgtt ttctgttcac agttgttttg taactgccta
     6901 ataagtaacc gcgctataac gaggtatcta ccgagattgt caattctcca gtatactgtt
     6961 ataaaaaaca aaactggagg atttttttat gtcctcccat cgtcccatcg actgttccgt
     7021 agctgcccaa taacggagta tagtcggctg aaacaagaat aggacaaaag tgcctcaaga
     7081 aaagcatcgt aatttgacaa tattattctg agatgttttt gatgtaagcc catattttct
     7141 caatgggatt atactcgggt gaataggtag gaagtggtaa cagtctatgc cctgctcctt
     7201 acacatgttc attctgtgaa accttgcatt gtccataatg ataagggatg gtgtatctaa
     7261 agtaggtagt aagaattttt ggaagcaagc ttcgaaaaag tcactcgtca tcgtatcttt
     7321 ataggtcatc ggagccatag tatattgaaa caaaaaaggg cattagtact tctataacag
     7381 tatcgcaatc tctggtgtgc gttgtctcga tcttaattca aataattata tcttcttcca
     7441 gagagactaa ctgcctgctc aaggctcgat catatttgag ataaaaatag gtctcaaccc
     7501 ctgtctcata aatataaata ggagtcaggt agcttaagtg attcaattct ttaaggaacc
     7561 gatttacttt ttctgggtct tgttcgcagt aggtacggct ctttttagac tatatcccat
     7621 agcctttaga gcgtaatgaa tagttgttgg aggacagttg aattcagcag ctatttcagt
     7681 caaataagca tcgggatgag tttcaatata gatctttaat ttatctctat caactttttc
     7741 aattaatagt ggaagttttc ttaaagttag tgtgttgaaa cgtttttata cagtgctaaa
     7801 atttgaagtg tagtgcagat gtatttgtaa tagtattata gcaattcttt ggagaaggtc
     7861 gagacaagag aaaataggta tctttgagat ataagtatga tttactataa aattattgag
     7921 ttattcataa aaatttctct atcaaatatc ataatgcgtc gtttaaggag atggttacaa
     7981 tgcaatatag ttgtggaaaa ataaatatta atatccctga tggatatgga gacattaagg
     8041 atatagtgtt ttcggctcat attatagtta ggtataataa tggacattgt ggaggtatag
     8101 atccgcatat aattggactt tgtaaaaagc agatcagaag gatgtctcca tatcctattt
     8161 tgataatcgt atctagagat tcaaaggtta ttgatgatta taaaaattta gatattgcct
     8221 atgttgattg tactcaatgt tcaaataatt ttgaaactgc cttacacgtt aaaaatattt
     8281 taaaattatt aaaaatccaa cttatacact gtcatggcta ctcaactaat tattttttat
     8341 atatgttaaa aaaacttgac aagaatggat ttggaaaggt taagacagtg ataacatgcc
     8401 atggatgggt tgaatataac ttaaaaaaga aattcttgac ttattttgat ttttggacat
     8461 attctatggg agatgctttt atttgtgtat ctgaaactat gaaaaaaaga ttggagagta
     8521 taataaaaaa ataagaagat agttgcaata aataacggaa ttaatgtatc aaatagcgat
     8581 ttagatgtag taggtgttca agactttaaa aaagaatttt gtattccaaa taacaaaaaa
     8641 atcatttgtt atgttggaag gttggatcca gaaaaaaggc cggatagatt ccttgaattt
     8701 gcagaaaaat tatttttagt cagagaagat gtgattttta ttatggctgg aaatggtagt
     8761 atgtgggcta ctttgaaaga aaaaatttgc catttgaaat gtagagataa tttcaggtta
     8821 cttggtgaaa tctatccagc tactatagtt taccaaatat cggatttatt gtatattcct
     8881 tctgatacag aaggaatacc tatgtgtgtt ttagagtcaa tgtcacaaga aactccggtt
     8941 ttagctagta atgttggcgg gttaagtgaa attattgaac atagggttga tggattttta
     9001 tttgagaagg aagatgttga gggagtgtgt gcttgtgcta attttttact caatgattct
     9061 gagtatttga aatatatagg tgagaatagt aaatcaaaaa taagaaaaca tttttctgtg
     9121 caaaaaatgt ttgtagaaac catgagagta tatgatgaat tattagagaa gagtagtcat
     9181 ggataggaat atagatcaag aattagtaag tattataata ccaacacata ataggtatga
     9241 atctctgatt agagctgtga aaagttgttt acatcaaagt tataaaaata tcgaagttat
     9301 aattattgat gataattata gtaatgtaaa tttaagaaat aaaattatcc atcaatttgg
     9361 ctataccagt catagaatta agttaatttt aagtaatgaa gatttaggtg caactaatgc
     9421 cagaaacata ggtatcaaaa attctagagg taagtatata tcatttttag acgatgatga
     9481 tgaatatatg ccagatcgaa ttttaaagtt gatggcttgt tttaaaaaga gtagaatgaa
     9541 gaatttagct ttagtttata gttatggaat aataatttat ccaataggaa atattttaat
     9601 ttattgagtt tcaacgagag aatattggtt acaaagaaat attattcttt aaacataaag
     9661 cggttactat taataggaga caaatgcaag gctttaaaag taatcaagaa ggcaagagaa
     9721 gaaaaaattt ttaacgaatt tctttttcga aatatatgtt attatatcat tttggttttt
     9781 tctattgtat atatgacaac tatgttcaat taaaatttag aaagtgagaa actattgtgt
     9841 atactattat aaaatcaaat ataaaaaaat ttagtttatt aacgatattt attgttgctg
     9901 gtcaattatt gctaatttat gcagcaacta ttaatgctct ggtgttgaat gaattaattg
     9961 cgatgaattt agagcggttt ttgaaattgt caatctacca aatgattgtc tggtgtggga
    10021 taatattcct tgactgggta gtgaaaaatt atcaggttga agtgatccaa gagtttaatc
    10081 tagagattcg aaatagagtt gccacagaca tctctaactc tacctatcaa gaatttcata
    10141 gtaaatcatc aggaacatat ctttcgtggc taaataatga tgttcagact ttaaatgatc
    10201 aggcttttaa acaacttttt ttagtaataa aaggaatttc tggtactata tttgcagttg
    10261 tgactcttaa tcactatcat tggtcattga ctgtagccac cttgttttca ttaatgatta
    10321 tgctacttgt accaaaaatc tttgcatcga aaatgcgaga agttagtcta aatttaacta
    10381 accaaaatga agctttttta aaatctagtg agactatatt gaatggattt gatgtgttag
    10441 cgtccttgaa tcttttatat gtattgccta agaaaattaa agaagcagga attttattaa
    10501 agatggttat acaaagaaag acaactgtag aaacgttagc aggcgctatt agcttctttc
    10561 tcaatatttt ttttcagata tctctcgttt ttttaacagg ctatcttgca ataaaaggaa
    10621 tagtgaaaat tggtactatt gaagcaatag gagcactaac aggtgttatt tttacagcgc
    10681 taggtgaatt aggaggtcaa ttatcctcta ttattggtac gaagcctatt tttttaaaat
    10741 tgtattcaat taatccaatt gagtcaaata aaatgaatga tatcgaacca aatgaggtga
    10801 atagagattt tccgttatat gaagcaaaaa atatttgcta taagtatgga gataaagaaa
    10861 tattaaaaaa cttaaatttt tgttttcaac gtaatgaaaa gtatttaatt ttaggtgaaa
    10921 gtggaagcgg gaaatctaca ttattaaaat tattgagtgg ctttttgaga gattatagtg
    10981 gagaattgcg attctgcggg gatgatataa aaaaaacctc ctatttaaat atggtttcga
    11041 atgttctata tgtagatcaa aaagcttatt tgtttgaagg tacgattaga gataatattt
    11101 tattggaaga aaattatact gatgaagaaa tactacagtc tttagagcaa gttggtttga
    11161 gtgtaaaaga ttttcctaat aacattttag attattatgt tggtgatgat gggagattac
    11221 tgtcaggagg gcagaaacaa aaaattactt tagctagagg gctaattaga aataagaaaa
    11281 tagtattaat tgacgaggga acttctgcta tcgataggag aacttcgtta gcgattgaac
    11341 gtaagatatt agatagagag gatttgactg tcattattgt tacccatgct ccgcatccgg
    11401 aacttaaaca atattttact aagatatatc aatttccaaa ggattttatt taagaatata
    11461 ggggagggaa acatgagatt ctattgtgaa gcttatgagg taattactga agagaaaaaa
    11521 gtatacttta atgatattga gttagaagtt aagtattcct ctgtcgaaga attgtttata
    11581 atatgtgaga aattattaga taagaaaaaa gtatcatttt tttatgtata tgagaaaccc
    11641 ttaaggtatc tgttatttga ctatatattt ctactagttc tagctaaaaa aaatatccca
    11701 atcttagatg gagtttccaa taagcaagtt gatccaacgt tgctgcatca cttttctttg
    11761 gaaattgaaa agaattttat cgatttttgc tataaaaata tggatctcat cctaaaaact
    11821 caaagtatat ctttgtgtca tagagaagag cttattattg tagatgtttc agatgactct
    11881 aaatttggtg ttttttacaa gcgttttaga acactggttg ataaaaataa cggaaaatat
    11941 gtttgtgttt ataatttcag tcgtgtttca gatatactgc aaaactggaa aaattattgc
    12001 aatcgaaaat tttcggttac gtttactgaa agccaatttg aattatttaa gcttttatat
    12061 aatcaaaaaa attttaaaac aatttcgttg ctatttggaa aaaaattgtt gctggaggaa
    12121 ttatctatta tagcgatctt actaatatag aatacttttg tattttttgg tgggatagta
    12181 tgtttgggaa agattctatt ggaaaatacg tatatgtaga agagattagt agatgtcatt
    12241 ttttagatag aaattattct ttttgttatg gtttacaaga ttataaaagt aaacttatta
    12301 aatatttttt agaatagtta atctattcga gagagataaa gaaatatgga aaatacttta
    12361 ttgaatatca caggaagctt cgatatggaa actaggaatc taattagtta ttctttaact
    12421 gatatctttg aaacagataa aataagaatt gaacttcttg gagaaattta ttataaaaat
    12481 ataaagttgg aattgcatga attcgctgga ttgtataaga tttatggaat tagcttaata
    12541 aagaatatta ccggaatgtt tttaattata atttttgata cgaagacaaa agaattaaaa
    12601 atttttcaag atataacgac ttcatacttt aatttatact atactgttta tggaggtgtg
    12661 ttctatatat agtacatctt taaagaaagt gatgaagtta tctcatgttc ccgttacctt
    12721 aaataacaaa aaaatacaag aatttatgag aaatggattt attttagatt caaatacttt
    12781 agtaaccgaa attaataaat tagaatattt ctcttacatt tctgtaaata atactcttcg
    12841 tatatgtggt attgattata atgattcaaa caattttact aaagaacaag tattaaagaa
    12901 ttgggactct atgctacgtg aatctatatt aagagtgtat agtgaagcag gagaggctaa
    12961 tattacacta tcatctggtt ttgattcaaa ttatatatta tatacattag cgaattatac
    13021 gaattcttca attaatgcat tttgcatagg aggagaaaaa ggaattaatg agattcctga
    13081 agtaactaaa attgcaaaat tttatggtat aaatttatta gttgatactg taatgagccc
    13141 aagatttaga atattttccc gatatagtat ggagattgga aggaagtctc tttgaatgtg
    13201 gtgtcatact gcagtactat ttaggcaggt tgttgttcca aaaagggaac acaagtattc
    13261 tttgtggcga gtcagcggat gaaataatga cttttaagta tcattctgtg aactacaatc
    13321 aattttgtaa tgataaacag aagtcagtat attttagtta ttccgactat ccattttatg
    13381 ttacaaattc aattgtttta aagaaaaata gcctactttt gcatagtttt ggcattcatc
    13441 ctagatatcc atataaaatg tctgagattg ttgaaatgtc taaaaaaata tcagatctaa
    13501 acgataaaaa agaatttcac aaaaagaact gtgagttgag gtttcaagat agtgttcttg
    13561 ataacataaa tagtgtcccg ggtacaactc atttatttag ttgtttaaat attcaaacat
    13621 tagtaaaaat tattttatat atttttcgat acaacagttg tatgaaaata tttaatttta
    13681 gagataaaga gcttatcttt tttaataaga ttgtaaatgg gctgatacaa aatattgagg
    13741 agaatttaga ggatgatatt gaaaggatat tgaaatattt atatatatgt ttatttaacg
    13801 aaatattcat tattaagaat aaggtgaatt tttttgatga tgtggaattt aatcagacac
    13861 ttagtgagtt tttagataaa ttgtagaggg aatggttatg agtgacaata ctaagtatat
    13921 ctttttatct ccacatttag atgacgcaat tttttcctgt ggtgattata tttcaaaatt
    13981 aacttcagaa ggagaaattg tacttgttat tactatattt tctggttacc ctttgagcca
    14041 acagctacag ccctctgcta agcaatttca taagttgtgt aaccttggga aatatccgat
    14101 tgaagaaagg aagaaagaag acaggttagc ttgtgaacgt ctacaatgtg attttagaca
    14161 cttatcgtac tatgaatgtc tctataggaa agatagaaat ggcaattttc tataccgtca
    14221 tatctatagt aaattaaaaa atgaggacac attaaagaat gatatcataa aagagctgtt
    14281 aatgcattta gacgacaaat gtgtcgtata ttgtccgctt tcacttggtg accatgttga
    14341 tcatgttttt gtgaatagta ttggaagggc gcttgaattt atgcgttata aagtaatata
    14401 ttatgaggac ttcccatatg taagtgatag tagtatggtt agttatatgg gaaaaactaa
    14461 agaattaaaa atgtatcaag aggaacttga tgaaaaacat tatattgata gaatatcatc
    14521 aatattgtgc tataaatctc agattttgat aatctggaaa tcagttgaaa aacttttaaa
    14581 caatattaaa gagttgtatt taaggaatgg ggcagcttat tctattagat tttggattaa
    14641 gaaataagga agtagtttta tgaatatagc agtaattggt ttggggcatg ttgggctggc
    14701 ctacgcgtta ctatttgcat ctaaatataa agttgttgca tatgatatag attctgtaaa
    14761 aataaataat ttaaaaaagg gcactcttcc atctaaaaat gaagagctta tgaagttttt
    14821 ttgcgagaat aacttaaata ttactttttt tgatacattt tctgaaatta aaaataatat
    14881 tgattattat attattgcgc ttccgacaga ttatgatgag aaaattggta gttttaatac
    14941 atatgaaatc gaacaaacgg tatcgaagat tctgagggta aaacctaatg gaaagattat
    15001 tttaaagtca acagttccga tcggcttttc aaacaaatta aaaaggctgt ttgatacaaa
    15061 aaatatcatt tttgtccctg aatttttgag agaaggttgt tctatatatg ataatttata
    15121 tccaagtcgc atagttgttg gagatgagac agttgaagga agaaaaattg cagagttgtt
    15181 cctttcgatt agtactcata gtactgccaa tattaaaaat gttatgttag tttctcctac
    15241 tgaagcagaa gcaattaagc ttttttctaa cacattctta gctctccgtg ttgctttttt
    15301 taatgaacta gattcttttg ctgagaggag aagtttaaat gctgaagttg taataaaagg
    15361 tgtttgttta gatccaagaa ttggaaattt ttataataat ccttcttttg gatttggagg
    15421 atattgtctt cccaaagata ctagacaatt aaaaaaagaa tttatagaaa taaatgcccc
    15481 agtgatagaa gcgattgata tttcaaacac aaatagaaaa cagttcatag ttaaacaaat
    15541 attggaacga aagccaaaga tagtaggaat atataaattg gggatgaaat ataattcaga
    15601 taattacaaa gagtcagcta ttttaagtat aattaatgaa ctattgattg tcggtataaa
    15661 aattttagta tatgaaccaa atttaaacgt tagtatagat aacgtaattt ttgaaaagaa
    15721 ttttgaacta tttataaaac aaagtgatct aattgttgca aatagatggg ataggggcct
    15781 tgaagcttat aaagataaag tttacacaag ggggatctgg ataagagatt aactattgat
    15841 attaaaaagg caataattta taaaatttgt acaaaaaaat gttaaatttt cacattttca
    15901 tagtatactg tttaatagaa agtaggttct ttgtaaccgc tccattataa aatcctttca
    15961 ctcttccggt atactattag gaaaaacata actggaggat attttgagct cacacctcat
    16021 cgttccagtg gctgttcccc aagctcgtgt actgcccaaa aaagttagac attctattta
    16081 gaacgaggat tgagttctgt attgtacaag gctcggtcct tttagagtca gcttaaggct
    16141 ggctttttca atcaccaaag tgtcagaatg ttttgacaaa tgaacacaaa taatgatata
    16201 atatgcaaaa gctaggaggt ggtaggatga ttgaaaaaat ggaactgggg gaattttaca
    16261 aggaattgcg cttggcgaga aagctcaagc agtcagatgt ggcttgtgct ggactaacag
    16321 cctctcagtt gtccaagttt gaactaggac agtctatgct gtctgcagac aagctgatcc
    16381 tagccatcca agggatcaat atgaattttg atgagtttgg gcacaaactc aacaactatc
    16441 aagaatctcc acatatgcga attggtagaa gggttgtgga ttgcttttcc catcaggata
    16501 tagctgcttt agagcagcta ttggaggaag tcgatcaaga acagatggcg cagacctatc
    16561 gtcgtttgaa tgccattgtg attaaaaatg cactccattc cttagataaa agctacccgc
    16621 taacagagga ggatagcgag tttttgacta cctacctcta cgctattgag tcttggacct
    16681 ggtttgaact ctatcttttt tgcaatacta tgcccttctt gagcaatcaa gatctgatct
    16741 ttttatcaac ctccttactc gaaaaatcca aggaatttaa agagttagta cacaatcgat
    16801 ggtatatgaa aagtggttat cttaatatca tctcagaact tatggagcgc aagctatttt
    16861 cttatattcc aatctttgaa gctgagctgg agcgcatgct ccgtccatac gatgtttttg
    16921 aaaaagtatt gtggcagttt ttaaagaaaa tgcagatttt tcttcaaacc aaaggaagca
    16981 atcaaaaaga gattgaacac tttatccaat ctctgcaagt attagaaaat ccacaattga
    17041 cagccctttt tgaattgcgt ttgcagcaat acaaagagct tatcgattag ttagaaatgg
    17101 gaaaaagata ataatatcag caacgatcgt caataatgtt aaaacttgtt tcttcatatt
    17161 acttccctcc tcattagtaa taactttatt ataccaggga aataatcaaa tctaccaaaa
    17221 tcgcaaatct gaaatttcta taagaaaaaa tatcaaatat gcgattttct aaaataagcc
    17281 aatttccgtg ttatactgtc cttgtaaaac atttgaagca aatcctaggt cgcagaagtg
    17341 gtttacaaat gaagataatt gaaggagtgt aaaagatgtt aaatttacaa tttgcagaaa
    17401 caatggaatt gacagaagct gagttgcaag atgttagagg aggcaacctc gtaaattcta
    17461 tgggaggtgg tggaagaagt ggtatatctg gttggggcgt cccaggaatt taccctggtt
    17521 ggggcaatca agggatgagt cctaatcgtg gagcttttga ttggactata gatttagcag
    17581 atggattgtt tggccgccgt agaagatagg ctcttatagg agataatagg atgaagaaaa
    17641 tgaaagaagt gaaatttcac ctagcaactg gcttgttgat tttgacttat tacctcattt
    17701 ttaatgtcac atcagactta gattttatgg ttgctttgtc tgataatatg tactatgtgt
    17761 tccaagtttt attggtactt attctgggaa ctatagctac aattgctttt gtcaaaagtg
    17821 aacattggaa agagtgtggt cgtttccagt ttcgttggtc ttacctgggt gttttccttc
    17881 tttctttttt cttattgttc gtgtgggcaa atctaacaac ctatatcttt cctcgtaccc
    17941 agaatggaag tacagtcgta gaagtagcca ccagtttaac aggaattagc tattttgtta
    18001 cgcgaatact ttatactagt attatcgcac ctgtatcaga agaggttgtt tgtcgtggtc
    18061 ttttgatgac aagtttatct aaagttaaga gatactatct tgatgttctt gtttccgctg
    18121 ccatatttgg cgctatgcat gttttgcagt atggttggat aacgacagat ttcataaagt
    18181 attttggaat gggcttgata ttttgtatga tgtttcgcta cacccgatcc atctattggg
    18241 ctatagctct gcatgcttca tggaatagct tcttactcat tgttacttta ttggtatttg
    18301 gatattagaa aggtgatata tgaaaaagat aatctcacac cgctacttta ttatagtttt
    18361 tctactagtt attgctgacc agaagttcag tgttttagtt ttacgcagcg accttgttgc
    18421 tgggctatct gactttgcct attatctgtc ggatatgatg ttgaattttc ttgtggtttt
    18481 atttgctctt attgctatga tttggtcggg gaaatggcag aaaatcaata gtagaaagtt
    18541 taaaggatcc tatcttttct attccttcct agctctgctt gcttttgttg cttggaattt
    18601 tgtcaccttt tttcttttcc cacctactcg aaatgaaatt tcttatcaac atgctgctcc
    18661 tacttttaca ggagctacgg catttttgat gtattttttt tatcctgtga ttgcaggtcc
    18721 catttttgaa gacatgattt atcgtggatt ggtgatgaca gctctggaaa aaggaaagaa
    18781 atgggggcta gatgtacttg gttctgctat tttatttggg gtctcgcaca ttagtaatca
    18841 cggttgggtc ttgacagact ttttcgtcta tatgggtggc ggacttatat ttgcagtctt
    18901 atttagaatg acaaagtcca tttattggcc tattgggctg catatagtct acaacggcat
    18961 tggtcagctt ttgatgttac tgtaattttg gttgttagta gtatcgcggt ctatttctgg
    19021 ggaaagattc ccatttgaac ttaagaagga gttgtgatga aagtatttct tcaaaataga
    19081 gattttaggc aattaactat caaccagtgg atttcaacgc ttggggatac gattttttat
    19141 ctggccttcc tgaattatgt ggcagatgcg tcttttgccc ctttggcgat tttacttatc
    19201 acgatttcag aaacccttcc ccaagttcta caaatctttc tgggagtttt ggcggatttt
    19261 caacatcatc gtgtcttgaa atacacagtc attagtttta caaaattttt gctttactct
    19321 atagtgtccc tatcgctttc agggcagtct ttttccttgt tgctagtaac ctttatttgt
    19381 ctgcttaacc tcttgtctga cacattgagt tatttttcag gcgccatgct cactccgatt
    19441 ttcattagaa ttattgggca agaccatctg gcagaagcta ttggatttaa acagtcaact
    19501 gttagtttag tgaaaacaat cagtaatatc ctaggaggag tcttactagg cattctatcc
    19561 atccagttta tttccttact gaacgctctt acctttttaa tcgcattttt aggtatcctc
    19621 ttcataaaaa ctgacctctt gaaagtagaa aaaacgatta gctatcaaga aggactctct
    19681 gtaaaatcct tttgccagca tttgctccaa tcatcgaaat tgatatggaa tatgaataag
    19741 gtgctcttgg ttttgtttat tatctctact agtcaagcag tgataaatgt tacggttcct
    19801 atttccactc tatttttaag gaaccagccc tttttgaatt tacaaactgg tcaatctctt
    19861 gccttgttag ccacactaga attatcagcc cttattttgg gaagtctgat gagtggctat
    19921 ctgcaacata ccatttccat aaaaatagcc ctgtatgctt cactcctact ccaattacta
    19981 ctcctagtgg gctttgcgac agctcatttt ggcttgattc tgatctttag ctttttggat
    20041 gcctttttcg cgggtattct ctctcctaga ttgcaagaac tcgtctttaa acaaatacct
    20101 gaggagtcaa tgggagcagt tcaatcttct atcggtgcca ttacggttgt tttacctagc
    20161 ttatttacaa tagctttggg aaccattgct actagctttg gaactctggc agttagcttt
    20221 gttttattac tatttcttct agttgccttt gtcatgcttc tgaatattcg tgaaagtatt
    20281 tagtggagaa tttctataat atatgtttga gactggattg ccagtctttt tatgatggtc
    20341 aaaatcaaca gactttttct cttttctgaa tgtgttatag tagactgaat ctaaaatagt
    20401 acgaaa
//
LOCUS       DAVBHS010000019        20281 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVBHS010000019 DAVBHS010000000
VERSION     DAVBHS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20281
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.019"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..153
                     /locus_tag="VQ651_000496"
     CDS             <1..153
                     /locus_tag="VQ651_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2753529.1"
                     /translation="EMFYGYEKSFRSLENLEQAIVDYIDYYNNKRIKVKLKELSPVQY
                     RTKSFG"
     gene            complement(201..2744)
                     /locus_tag="VQ651_000497"
     CDS             complement(201..2744)
                     /locus_tag="VQ651_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HET2753530.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQKKVD
                     ILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTKTFT
                     EAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQAQT
                     GFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(2806..4428)
                     /locus_tag="VQ651_000498"
     CDS             complement(2806..4428)
                     /locus_tag="VQ651_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2753531.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            4634..5659
                     /gene="trpS"
                     /locus_tag="VQ651_000499"
     CDS             4634..5659
                     /gene="trpS"
                     /locus_tag="VQ651_000499"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET2753532.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            5812..7290
                     /gene="guaB"
                     /locus_tag="VQ651_000500"
     CDS             5812..7290
                     /gene="guaB"
                     /locus_tag="VQ651_000500"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET2753533.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(7341..8438)
                     /gene="recF"
                     /locus_tag="VQ651_000501"
     CDS             complement(7341..8438)
                     /gene="recF"
                     /locus_tag="VQ651_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET2753534.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(8441..8809)
                     /gene="yaaA"
                     /locus_tag="VQ651_000502"
     CDS             complement(8441..8809)
                     /gene="yaaA"
                     /locus_tag="VQ651_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HET2753535.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKIDIPDMNIDILLTQSTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            8968..10218
                     /locus_tag="VQ651_000503"
     CDS             8968..10218
                     /locus_tag="VQ651_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET2753536.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSYLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHCPSKYGDEKHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSQLDLFSGFLRMYAGIDRENRNQARKMMNNQLLDL
                     KKGYFTELELEQTKEMIRRSLLLSQDNQSSLIERAYQNALLGKSSADFKSWIAKLEQV
                     DKDAICRAANNVKLQAIYFMEGIE"
     gene            10215..11498
                     /locus_tag="VQ651_000504"
     CDS             10215..11498
                     /locus_tag="VQ651_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET2753537.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTLVTEVDGDVKQYPAGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELITSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKLTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            11540..12361
                     /locus_tag="VQ651_000505"
     CDS             11540..12361
                     /locus_tag="VQ651_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2753538.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            12372..12917
                     /gene="pgsA"
                     /locus_tag="VQ651_000506"
     CDS             12372..12917
                     /gene="pgsA"
                     /locus_tag="VQ651_000506"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET2753539.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            12914..13741
                     /locus_tag="VQ651_000507"
     CDS             12914..13741
                     /locus_tag="VQ651_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET2753540.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            13726..14565
                     /locus_tag="VQ651_000508"
     CDS             13726..14565
                     /locus_tag="VQ651_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET2753541.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPNQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            14558..15352
                     /locus_tag="VQ651_000509"
     CDS             14558..15352
                     /locus_tag="VQ651_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2753542.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            15411..16229
                     /gene="mreC"
                     /locus_tag="VQ651_000510"
     CDS             15411..16229
                     /gene="mreC"
                     /locus_tag="VQ651_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET2753543.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKVSQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            16229..16723
                     /gene="mreD"
                     /locus_tag="VQ651_000511"
     CDS             16229..16723
                     /gene="mreD"
                     /locus_tag="VQ651_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET2753544.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            16817..17995
                     /locus_tag="VQ651_000512"
     CDS             16817..17995
                     /locus_tag="VQ651_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET2753545.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            18219..18998
                     /gene="rpsB"
                     /locus_tag="VQ651_000513"
     CDS             18219..18998
                     /gene="rpsB"
                     /locus_tag="VQ651_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET2753546.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            19077..20117
                     /locus_tag="VQ651_000514"
     CDS             19077..20117
                     /locus_tag="VQ651_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HET2753547.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
BASE COUNT         6467 a         3234 c         4195 g         6385 t
ORIGIN      
        1 gaaatgtttt acggttatga gaagagcttt agatctttag aaaaccttga acaagctatt
       61 gtggactaca ttgattacta caacaacaaa cgaattaagg taaaactaaa agaacttagc
      121 cctgtgcaat acagaactaa atccttcgga taaattaatt gtctaacttt ttggggtcag
      181 tacattaact ggtcgatttt ttatgcctta gatgactttc gtctgtgatt atagattcca
      241 aatagtaaga gagaagtaaa ggaacagatt gctccagtaa taaaaccatt gggaatgaag
      301 gaaagtgtaa tagttccttt ccccttggga atgtcaactt tcataaatcc agtttgagct
      361 tgtttaattt ctattttctt accatcttgg taggcagacc aacctttgtc ataaggaatg
      421 gtgaagaaaa tagatgtatc ttgttggaca tcatatgtag caaaaacctt gtttttagaa
      481 gttgatactg tgacaggttg ttctttaatt ttttgaattg cctcggtgaa agttttggta
      541 tctaaacgat agaaggtagg agattcaaat gatacttgtg aatttccagg gaaactaaca
      601 ttgatattga aagttttttt ctctttagta tatcctagat taaagaagga gaagacatta
      661 tcagttgtaa aagtcttttt ttcaccattt acaaggatgt caaccttctt ttgtttatcg
      721 ttagaaaagt gaaggtttat gaaagagaga taaacttggc tgttttctgg aacttcaatt
      781 tgatactgga ttgctgcatc ttcatttgaa gaacttgtga cactaatcaa atcattagta
      841 ttttctgttt tttcataagg tattggagaa aaataatcaa aattgacgtt agcaagttga
      901 tttaaaaatg aggcctgatt atccaaggta tgttcattga acttgacatc attgtaaaca
      961 gattgactag caactgcaat cggaagagag tattgatttt catatagggt aagattatct
     1021 ttttgataga tatctttaaa gccatactta tcaataggac tgtctgagat attgtactgg
     1081 ataccaaata aactatcagc caaaatacta ttatttgcat atcggagatt gagattagtc
     1141 ccagaggatt taaaaccaag tttatctaaa gtagagcttg atgaacgatt tcgaacagat
     1201 gaaaattgag agattccatt gtagttgaat ttcatactgt catttcctgt ctgagtttgt
     1261 agtttttcag tacgagtaaa ttgatttcca atatatgttg agaaagattc catagctggg
     1321 atatctcgac tataagcact tcgagaagca aatccccatt ccttagcaat tccgtccatt
     1381 tgagatgaag catttaaact catttcaacc agtataaata aagagattag aatggcaaat
     1441 agattcacag atataaactt tttgataact gcaaggagta aaagagaata gacaaccaaa
     1501 aattcaagag taagcagaat attcaaatct gttaaaaaag aataatgcga ttttagatag
     1561 atggtagcta aaaatcctgc tactacaaga aaaagcgaaa ctaaaaaatt ccagacttta
     1621 agttctttca gacgctttaa gacttctgct gctgtgtaaa ttaacaaggt agagaaaatc
     1681 caagcatagc gatgtaaaaa catgtttgga gtatgcatgc cttgccaaaa taagtcaaga
     1741 gcttctatgt aaaagcttgc aattagaaat gcaaagaata ttacatatat gagtttcacg
     1801 tgaaacttaa tagatttcag cgtaaaaaat aaaatggtca aaataaaggg aaatagtcca
     1861 acaaaaatca ttgggatggc cccatacttt gttgtgtcaa aggaaccaat gaattgctta
     1921 gcaaagagat caagatacca gctactttca gtttgaaact ttgtaacttc agtcaatttt
     1981 tccccatgtg tctgtaaatc aaatagagtg ggaagagtca taatcaaact agccatacca
     2041 gctaaaaagg agataactat gaaatcaaga acagatgatt ttcgagtctt aaagtcccac
     2101 gaaatttgac agagatacca gaaaataaga aacaatactg tcatatatcc aaaataataa
     2161 ttttgaataa ataagattga cagacttgta aagtacaata ggagtttctt ttcagttatc
     2221 agtagatgta aaccagttat aattaaagga atcaagataa aaacatctag ccaggttttt
     2281 atctctaatt gactgacagt gaaactcatc agagcatagg aagtagataa ggctagtttt
     2341 aaagtctgag ggatagattg aaacaattta ttcaaactaa aaaaggttga cagaccaatc
     2401 aatccaaatt ttaagagagt ggtcagatag acagcatctg gcatattcgt tagatcaaaa
     2461 aagtaaacta gaggcgcgag aaaactaccc aagtaataac tagatagggc atagaagttt
     2521 agccctagac cacttgtaaa ggtgtaaaac agactactat ttccatgtag gatatttcgt
     2581 aaggctacat caaaaataac gtattgatga aagccatctc ctaatagagg agagttgtcg
     2641 ctattccagt agataccttg agatagatat actccagaca taatcactac aggaatgatg
     2701 aaagaaataa aataggttcg atatgttttt aaaaatgatt tcatgttacc tcgtagaatg
     2761 atagaaaact cagttggtta acccaactga gttttgaagt tttatttagt ctttccaaag
     2821 ttctttaact tttgcttgta cttctgcatt ttctaggaat tcatcgtagg tttcatcgat
     2881 acggtcaatg acgccatttt tagataagac aatgatatgg ttagccaaag tttgaataaa
     2941 ctcgtggtca tggctggcaa agatgattga ttctttaaag tttttcagtc catcattcaa
     3001 gcttgagata gattccaagt ccaagtgatt tgttggatca tcaagtacaa ggacatttga
     3061 ttttaagagc atgagttttg aaagcatgac acgaactttt tctccccctg acaagacatt
     3121 tacaggtttg ttaacttcat ctccagagaa gagcatacgg ccgaggaaac cacgtaggaa
     3181 agtattgtca tcttcttctt tacttgcgaa ttgacgcaac cagtcaagaa ttgattctcc
     3241 tcctgcaaaa tcagctgagt tatcttttgg taggtaagat tgactagttg taactcccca
     3301 cttgacagtt ccttcatagt caatatctcc catgattgca cgaattaatg cagtcgtttg
     3361 aatatcattt tgtccaataa gtgctgtctt atcatctgga cgcaagatga aactaatatt
     3421 atccaagata gtttcaccat caatctttac agttagattt tctactgtca agagatcatt
     3481 accaatctca cgttccgctt taaagttgat aaatggatat ttacgactag atggtacaat
     3541 ctcttctagc tcaatcttat caagcatttt cttacgtgat gttgcctgcc ttgacttaga
     3601 agcattggca gagaaacgag caacgaattc ttgcaattgt ttaatttttt cttctgcttt
     3661 agcattacgg tctgctagca atttagcagc aagctcagaa gattccttcc agaagtcgta
     3721 gtttccgaca tagagtttga tttttccaaa gtcaaggtcg gccatgtgag tacaaacttt
     3781 gtttaagaag tgacggtcgt gggatactac gataactgtg ttatcaaagt caatcaagaa
     3841 gtcttctaac caagtaatcg attggatatc caaaccgtta gtaggctcgt ccaagagaag
     3901 aacatctggt ttaccaaaaa gtgctttggc gaggagaacc tttacttttt caccgttggc
     3961 caattcgctc atgttttggt agtgtaattc ttctggaatg tttaggtttt gaagtagttg
     4021 agaggcttca ctctctgctt cccaacctcc aagttcggca aactctcctt cgagttcggc
     4081 agcacgaacc ccgtcctcgt ctgagaaatc ttccttcatg tagatagcat ctttctcttt
     4141 catgatgcta taaagttttt catttcccat gataacgaca tcaatggcac gttcatcttc
     4201 gtagtcaaag tgattttgac gaagaacaga gagacgttca tctggaccaa gagagatgtg
     4261 accagtagta ggttcgatat ctccagctaa aatttttaaa aaggttgatt ttccggcacc
     4321 attagcaccg attaatccgt aagtatttcc ttctgtaaat ttgatattga catcatcaaa
     4381 aagtttgcga tcactaaaac gtagtgaaac atcagatact gtaagcaatg tttttctcct
     4441 atatgtgtaa tatatttatt ctactagaaa atacagaaat attcaaattt ttatttgtca
     4501 attttgtgta aattatattt acagtatcct ttacacaaat ctgtaaaaag caaggctgat
     4561 ttattttgat aaattacggt tatttcatta aaaaaatgct ataattgaaa ggactatatc
     4621 gaaggagaac aaaatgacta aacccattat tttaacagga gaccgtccaa caggaaaatt
     4681 gcatattgga cattatgttg gaagtctcaa aaatcgagta ttattacagg aagaggataa
     4741 gtatgatatg tttgtgttct tggctgacca acaagccttg acagatcatg ccaaagatcc
     4801 tcaaaccatt gtagagtcta tcggaaatgt ggctttggat tatcttgcag ttggattgga
     4861 tccaaataag tcaactattt ttattcaaag ccagattcca gagttggctg agttgtctat
     4921 gtattatatg aatctagttt cgttagcacg tttggagcga aatccaacag tcaagacaga
     4981 gatttctcag aaaggatttg gagaaagcat tccgacagga ttcttggtct atccaatcgc
     5041 tcaagcagct gatatcacag ctttcaaggc taattatgtt cctgttggga cagatcagaa
     5101 accaatgatt gagcaaactc gtgaaattgt tcgttctttt aacaatgcat ataactgtga
     5161 tgtcttggta gagccggaag gtatttatcc agaaaatgag agagcagggc gtttgcctgg
     5221 tttagatgga aatgctaaaa tgtctaaatc actaaataat ggtatttatt tagctgatga
     5281 tgcggatact ttgcgtaaaa aagtaatgag tatgtataca gatccagatc atatccgcgt
     5341 tgaggatcca ggtaagattg agggaaatat ggttttccat tatctagatg tttttggtcg
     5401 tccagaagat gctcaagaaa ttgctgatat gaaagaacgt tatcaacgag gtggtcttgg
     5461 tgatgtgaag accaagcgtt atctacttga aatattagaa cgtgaactgg gtcctattcg
     5521 tgagcgccgt attgaatttg ctaaggatat gggagaagtt tataatatga ttcaaaaagg
     5581 tagtgaaaga gcgcgtgaag ttgcgggtca aaccctatct gaggtaaaag gggcaatggg
     5641 acttcattac tttaactaag tttattttac aagaaactat catttctatc tcgaagaaaa
     5701 gatagttttt ttatttaccc ctgtaacatt tgacaaattt ttatagaatg gtatgataga
     5761 tacaatataa aaagagtcaa gctcaaaaag aaagaaaaga ggaaacttcg aatgtctaat
     5821 tgggacacta aatttttgaa aaaaggtttt acctttgatg atgtattgct tattccagct
     5881 gaaagtcatg tgttgcctaa cgatgcagat ttaacaacta aattggcaga taatttgact
     5941 ttaaatatcc caattattac cgctgccatg gacacagtta cagagagtca aatggccatt
     6001 gctattgctc gtgcaggcgg tctcggagtt atccataaaa acatgtcaat tgctcaacaa
     6061 gcagacgagg ttcgtaaggt aaaacgttct gaaaatggag ttattattga tccgttcttc
     6121 ttgacgcctg aacatacaat tgctgaagca gatgagctta tgggtcgtta ccgcatcagt
     6181 ggtgttccag ttgttgaaac acttgaaaat cgtaaattgg ttggtatttt gacaaaccga
     6241 gatcttcgtt ttatttcaga ttataatcaa ccaatttcaa accatatgac tagtgaaaat
     6301 cttgttactg ctcctgtggg tacggatctt gcaacggctg agagtattct tcaagagcat
     6361 cgtattgaaa aacttccgtt ggtcgatgaa gaaggcagtc tttctggttt gatcactatc
     6421 aaagatattg aaaaagttat tgagtttcca aatgcggcta aagatgagtt tggtcgtctt
     6481 ctagttgcag gtgcagtagg tgttacttca gatacatttg aacgtgcaga ggctcttttt
     6541 gaggcaggag cggatgcgat tgttattgat actgcacatg gtcattctgc aggtgtcttg
     6601 cgtaaaattg ccgagattcg tgctcatttc ccagatcgga ctttgattgc tggaaatatt
     6661 gctactgctg aaggtgcacg tgccctttat gaagcgggtg tagacgttgt taaggttggt
     6721 attggaccag gttctatctg tactactcgt gtgattgctg gtgttggtgt tccgcaagta
     6781 acagctatct acgatgctgc agctgttgcg cgcgaatatg gtaaaacgat tattgctgac
     6841 ggtgggatca agtattctgg agatattgta aaagcacttg ctgcaggtgg aaatgctgtt
     6901 atgcttggat ctatgtttgc tggaactgat gaagctccag gcgaaactga aatcttccaa
     6961 ggacgtaaat tcaagactta ccgtggtatg ggatcaattg ctgctatgaa gaaaggttca
     7021 agcgaccgtt atttccaagg ttctgtcaat gaagcaaaca agcttgttcc agaaggaatt
     7081 gaaggtcgtg ttgcttataa aggagcggca gctgatattg ttttccaaat gattggtggt
     7141 attcgctctg gtatgggtta ctgtggtgca gctaacctta aagaactaca cgataatgct
     7201 caatttattg aaatgtctgg tgctggtttg aaagaaagcc atcctcatga tgtgcaaatt
     7261 actaatgagg caccaaatta ttctatgtaa aaaacaatga aaagaactcc agtgaaaaca
     7321 ggagttcttt tacaatgttg tcaatttcca tttacagcag ctttaccatc ctgaatagtg
     7381 aagatactta gattttctgg cagattttga agatggtcta agcttgttgt tgtgataaag
     7441 gtttggattg attgagaaat cgtttctaat aattttaact gtctagtgtt gtcaagttca
     7501 ctcatcacat cgtcaagcag taatatagga gattctgtgg taatgctttc cattaattcg
     7561 atttctgcta attttatcga gaggacgaga ctacgatgtt gaccttggct tccgaaacta
     7621 gcatccatcc catttatata aaaagaaatg tcatctcgat gaggaccgac accagtattc
     7681 tttttaaata aatctctgga tctacttttt tctaaagcaa ttttgaaaga ttcggataag
     7741 ttttgtttgt cagttatatt gacagaagat tgataggata ttgacaactc ttcgatctga
     7801 ttagagagtt caaaatgttt cttacgccca aatgattcta gtttttttat gaaatctaag
     7861 cggtgattca ttacacgaca tccataatca actagctgat catctaacac agaaaggaat
     7921 gtttcatcta ttttttgagc tgattttagg taagtgtttc tttgctttag gatgtggtta
     7981 taattggtta agtcagataa atagattggc ttaatttgcc caagttccat atcaatgaat
     8041 tttcgtcgaa tcgaaggtgc tcctttaatt agttgtaaat cttcaggagc aaataagaca
     8101 acattcatgt gtcctacata atctgaaagg cgtgcctgtt ttaagtgatt aacttttgtt
     8161 acacggcctt tttgtgttag ttcgatttct aggggaatgg atccagtttt tttctgaacg
     8221 agacctgaaa gatgaagttg ttcctcatca aaatgaatga gatttttatc tgttcgagtt
     8281 cgatgactac gcgttaaggc taaaaaatag atagcctcta acatgtttgt ttttccttgt
     8341 gcattacgtc ccaaaaagac attcaattta ggattaaagt ctattttcgt ttctttgtaa
     8401 ttacgaaaag tcttgagaga tagatgttgt agccacatga ttatctacca gggaatcgtg
     8461 gagcttgttg gcttttaggt gatgaagcag gtttcgattt gtctttttta actcccttat
     8521 tcatcttttt gacaagttta gcgatccgtt ctttttcaac tttatcagct tggtattcat
     8581 cttgttcttc agaagtagat tgtgtcaaca agatgtcaat gttcatgtca gggatgtcaa
     8641 ttttatcgcc aatacgaagt tttttaccac gacgactctc taattcccta ttaaagtaaa
     8701 cagaatgttc agagagaaat gatttaatag ctcctccgct atgtgtaatt ccaagttctt
     8761 tgagtagtgc ttggagggta ataaattctt caaataattt gtattccata attcacctca
     8821 atctttggta ttataccata ttttcctaaa aatagtgaga gatacagcga gaaatgtaag
     8881 cgatttttat ttcaaaagtg ttacagggaa caagaaaatt tcaggttttc gtgatataat
     8941 agaagtctgt atataaggag ataaatcatg gagttagtgc atggaatttc aacacatttt
     9001 atccaatcaa aaaagtttaa aacaaacaaa attaccgtgc gttttaccgc tccattatcc
     9061 cttgatacga ttgcaggtca catgttgagt gcaagtatgc tagagactgc taatcagatg
     9121 taccccactt ctcaagattt gaggagacac ttggccagtc tatacggtac agatatgtca
     9181 accaattgtt tcagaagagg gcaaagccac attatagaat tgacatttac ctatgttcgt
     9241 gatgagtttt taagtaggaa aaacgtgcta acttctcaga ttttggaact tgtaaaagaa
     9301 actctttttt cacccgtagt agttgataat gggtttgatc cggccttatt tgaaattgag
     9361 aaaaaacaat tgctagcaag tttagcagct gatatggatg attcttttta ttttgcacat
     9421 aaagaattgg ataaattgtt ttttcatgat gaacgtcttc aattggaata tagttattta
     9481 cgaaatcgta ttttagctga aactccacaa agttcttatt cttgtttcca agaattttta
     9541 gccaatgatc gaatagattt ctttttccta ggtgatttta atgaggttga aattcaaaat
     9601 gtattagaat catttggctt taaaggtcga aaaggagatg tgaaggttca gtattgtcaa
     9661 ccttattcta atatccttca ggaaggtatg gttcggaaaa atgtgggaca atccattttg
     9721 gaattaggtt atcattgccc ttctaaatat ggtgatgaga aacatttacc catgattgta
     9781 atgaatggtt tacttggtgg atttgctcac tctaaacttt ttacaaatgt ccgtgaaaat
     9841 gctggattag cttatactat ttcaagtcag ctcgatttat ttagtggatt cttgaggatg
     9901 tatgctggta ttgaccgaga aaatcgtaac caggctcgta aaatgatgaa taatcaactg
     9961 cttgatttaa aaaaaggtta ttttacagag cttgagttag agcagaccaa ggaaatgatt
    10021 cgtcggtctt tgttactttc tcaagataac caatcttcat tgattgaacg tgcttatcaa
    10081 aatgccttac ttggaaaatc ttcagcagat tttaaaagtt ggattgcaaa actcgagcaa
    10141 gttgacaaag atgctatttg tagagcagct aacaatgtga aactacaagc gatttacttt
    10201 atggaaggaa tagaatgaca aaggttgttt ttgaagaaaa atactatcca gctgtaaaag
    10261 aaatggttta tcgaactcgt ttggcaaatg gattgacagt agctcttttg cctaaaaagg
    10321 aatttaaaga ggtttacggg agtgtaactg tacagtttgg ttcggtagat acgcttgtca
    10381 cagaagttga tggagatgta aaacaatatc ctgcaggaat tgctcatttt cttgaacata
    10441 aattatttga aagagaagat tctagcgatt tgatgtcggc ttttacgagt ctaggtgcag
    10501 atagtaatgc ctttacaagc tttacaaaaa caaactatct tttttcagca acggattatt
    10561 ttttagaaaa tttagattta cttgatgaat tgataacatc agcacacttt actgaagctt
    10621 ccattctgac agagcaggat attattcagc aagaacgaga aatgtaccaa gatgatccag
    10681 attcgtgttt attcttttca actttagcga atttgtatcc tggtacacct ttagcaactg
    10741 atatagttgg aagtgaggag tccatttccc aaatcaatct aactaatttg caagaaaatt
    10801 ttacaaagtt ttacaaacct gtaaacatgt ctctgttttt agttggtaat tttgatgtgg
    10861 agcgagtaca ggactatttt gaaagcaaag aactgaaaga ttcagatttt caggaagtag
    10921 caagagaaaa gttgttttta cagcctgtaa agctaacaga tagtatgaga atggaagtat
    10981 cttctcccaa actagcgatt ggagttagag gtaagcgaga agtttctgaa gcggattgct
    11041 atcgacatca tattttatta aaattattgt ttgcaatgat gtttggttgg acttcggatc
    11101 gttttcaaaa atgttatgaa tcaggtaaaa ttgatgcgtc cttatctctg gaagttgaaa
    11161 taacaagtcg ctttcatttt gtcatgttga caatggatac gaaagagcca gttgctttgt
    11221 ctcatcaatt taggaaggct attcgtaatt ttacaaagga tttagatatt acagaggaac
    11281 atttagatat tatcaaaaga gagatgtttg gcgaattttt cagtagcatg aactctcttg
    11341 aatttattgc aacgcaatat gatgcttttg aaaatggtga gacaattttt gatttgccga
    11401 aaattttaca ggaaattact ttagaggatg tccttgatgc tggacatcat ttaatagatg
    11461 atggtgacat agttgatttt acaatatttc catcgtagta acctatcata atagacacta
    11521 gaaagaaggg atgacaagta tgagaaaaaa aacaattgga gaggttttac gattagctag
    11581 aatcaatcag ggattgagtt tagatgaatt gcagaaaaag acagaaatcc agttagatat
    11641 gttggaagca atggaagcag acgatttcga tcaacttcca agtccttttt acacgcgttc
    11701 tttcttgaaa aaatatgcat gggctgttga gttagatgac caaattgttt tggatgctta
    11761 tgattctggg agtatgatta cttatgagga agtagatgtt gatgaagatg agttgacagg
    11821 tcgtagacgt tcaagtaaga aaaagaagaa aaaaacatca tttttacctt tattttattt
    11881 tatccttttt gctttatcga ttttaatttt tgtgacttat tatgtttgga actatattca
    11941 aactcaacca gaggagcctt ctctttctaa ttacagtgtg gttcaatcaa caagttcaac
    12001 tagctctgtt ccccactcct caagtagtag ttcttctagt atagaatcag ctataagtgt
    12061 atcaggcgaa ggaaatcatg tagaaatcgc ttataagaca agtaaggaaa cagttaaatt
    12121 gcaattggca gtttcagatg ttacaagttg ggtcagtgtt tcagaaagcg aacttgaggg
    12181 cggtgtaacc ttatcgccaa agaagaaaag tgcagaagca acagttgcaa ctaaaagtcc
    12241 tgtaacaatt acgttaggtg ttgtaaaagg tgttgatttg acagtagata atcagactgt
    12301 tgatttatcg aaattaacag ctcagactgg acaaatcact gtaaccttta ctaaaaatta
    12361 aggaaaaacg aatgaaaaaa gaacaaattc ccaatctctt aacaataggt cgaattctct
    12421 ttatacctat ttttatcttt attttaacga taggaaattc gatagagagt catatagttg
    12481 cagctattat ctttgctgtt gccagtatta ccgactattt agatggatat ttagctcgta
    12541 aatggaatgt ggtcagtaat tttggtaaat ttgcagatcc tatggcggat aagttactag
    12601 ttatgtcggc ttttattatg ttgattgagt taggtatggc tccggcttgg attgttgcag
    12661 tgattatatg tcgtgagtta gctgtgacag gtttaaggct tttattggtt gaaactggtg
    12721 gaacaatttt agcagcagca atgcctggaa aaattaaaac ttttagtcag atgtttgcta
    12781 ttattttctt gctattacat tggactttgc ttggtcaagt tctactttat gtagccttat
    12841 ttttcactat ctactctggc tatgactatt ttaagggtag tgcctatgta tttaaaggga
    12901 catttggttc gaaatgaaat caataattga tgtaaaaaat ctttcttttc gctataaaga
    12961 aaatcagaac tactacgatg tgaaggatat tacgtttcac gtgaaacgtg gagaatggct
    13021 ttcgattgta gggcataatg gtagtggtaa atcaacgacg gttcgattaa ttgatggctt
    13081 actggaagca gaatccggag agattgtaat tgatggccaa cgtttgactg aggaaaatgt
    13141 ttggaatata cgtcgtcaaa tcggtatggt ttttcaaaat ccagacaatc aatttgttgg
    13201 agcgactgtt gaagatgatg ttgcttttgg tttggaaaat cagggacttt ctcgtcaaga
    13261 aatgaaaaag agagtggaag aagctctggc tttagttggc atgttggact ttaaaaagag
    13321 agagccagcg cgtctatcag gtggtcaaaa gcaacgtgtg gccattgcag gtgttgtagc
    13381 cctaagacca gctattttaa tcttagatga agcaacgagt atgttggatc ctgaggggcg
    13441 tagagaactt attgggacag taaaaggaat tcgaaaagac tatgatatga cggtcatttc
    13501 tattacccat gatttggaag aagtcgccat gagtgatcgc gtattggtca tgaaaaaagg
    13561 ggaaattgaa tcaactagta gtccaaggga gcttttctct cgaaatgatt tagatcaaat
    13621 tggattagac gatccttttg ctaatcaatt aaaaaaatct ttgagccaga atggctatga
    13681 tttacctgaa aattatttga cagaaagtga gctagaggat aagctatggg aattgctcta
    13741 gaaaatgtga attttacata tcaagaaggt actcccttag cttcagcagc tttgtcggat
    13801 gtttctttga cgattgaaga tggctcttat acagctttaa ttgggcacac aggtagtggt
    13861 aaatcaacta ttttacaact cttaaatggt ttattggtgc caaatcaagg gagtgtgagg
    13921 gtttttgata ccttaatcac ctcgacttct aaaaataaag atattcgtca aattagaaaa
    13981 caggttggct tggtatttca gtttgctgaa aatcagattt ttgaagaaac ggttttgaag
    14041 gacgttgctt ttggaccgca aaattttgga gtttctgaag aagatgctgt gaagactgcg
    14101 cgtgagaaac tggctctggt tggaattgat gaatcacttt ttgatcgtag tccgtttgag
    14161 ctgtcagggg gacaaatgag acgtgttgcc attgcaggca tacttgccat ggagccagct
    14221 atattagtct tagatgagcc aacagctggt ctagatcctc tagggagaaa agagttgatg
    14281 accctgttca aaaaactcca ccagtcaggg atgaccatcg tcttggtaac gcatttgatg
    14341 gatgatgttg ctgaatatgc gaatcaagtc tatgtaatgg aaaagggacg tttagtaaag
    14401 gggggcaaac caagtgatgt ctttcaagac gttgttttta tggaagaagt tcagttggga
    14461 gtacctaaaa ttacggcctt ttgtaaacga ttggctgata gaggcgtgtc atttaaacga
    14521 ttaccgatta agatagagga gttcaaggag tcgctaaatg gatagtatga ttttagggcg
    14581 ttatatccca ggggattcga ttgttcaccg attggatcca cgtagcaaat tgcttgctat
    14641 gatgctactg attttgattg tgttttgggc taataacccc ttgacgaatc tgattctttt
    14701 tatagcgaca gggatattta ttgccttgtc aggagtatct ctttcatttt ttattcaggg
    14761 cttgaagtct atgtttttct tgattgcctt cacaactatt tttcaactat ttttcatttc
    14821 taatgggaat gttttatttg agttttcgtt tgtgagaatc acggattatg ctttgcaaca
    14881 agctgggatt attttttgtc gctttgtatt gattattttc ttttcaactt tgttaacctt
    14941 aacaaccatg cctttaagtt tggcatcagc tgtcgaagct ttattagcac ctttaaagcg
    15001 tgtgaaagtt ccagttcatg aaattggatt gatgctgtct atgagtttgc gttttgtccc
    15061 aaccttgatg gatgatacga ctcggattat gaatgcacag aaagctcgtg gagtggattt
    15121 tggagaagga agcatcgttc aaaaagtaaa ggcgatgatt cccattttga ttcctctttt
    15181 tgcgacaagt ttaaaacgtg cagattcctt ggctatcgct atggaagcgc gtggctatca
    15241 gggtggaaaa ggcagaagtc aataccgaca attgaaatgg actctaaagg atacgctgac
    15301 cattcttgtt attctcgtac ttggttgttg tttatttttc ttaaaatctt agtagctttc
    15361 aggaattgat aaaaagttac tgtaacagtt tttgatataa aggtaggaat atgaaccgtt
    15421 ttaaaaaatc aaaatatgtc attattgttt ttgtcactgt tctgcttgtg tcagctctct
    15481 tagcgacgac ttattcaagt acaattgtga caaaattagg agatggaatc tcattggttg
    15541 atagagttgt acaaaaacct tttcagtggt ttgattctgt caaatcagat ttggctcatt
    15601 tgacacgaac atataatgaa aatgaaagtt tgaagaaaca gctttaccaa ttagaagtta
    15661 aatcaaatga ggtggaaagt ttaaagacag aaaatgaaca actgcgccaa ttgcttgata
    15721 tgaagtctaa attgcaagcc acaaagactt tagcagcaga tgttattatg cgttctccgg
    15781 tatcttggaa gcaggagttg accttagatg caggtagatc aaaaggtgct tctgagaaca
    15841 tgttagctat tgcaaatggt ggcttgattg ggagtgtttc aaaagtagag gagaactcta
    15901 ctatagtcaa ccttctgaca aatacggaaa atgctgataa gatttctgtt aaaattcaac
    15961 atggctctac tacaatttat ggaattatta ttggctatga caaggaaaat gacgttctta
    16021 aagttagcca attaaatagt aatagcgata ttagtgcggg agataaggtg actactggtg
    16081 gattaggaaa ctttaacgtt gctgatattc ctgttggtga agtggttgcc acaacgcata
    16141 gtacagacta tttgacacga gaagtaactg ttaaattgag tgcagatact cataatgtag
    16201 atgtgataga attagtgggg aattcataat gagacagttg aagcgagttg gagtattttt
    16261 attgcttcct ttctttgttc taattgacgc ccatattagc cagcttctgg gctcattttt
    16321 cccccatgta catttggcta gtcattttct ttttctattt ctcttatttg agacgataga
    16381 agtatcagag tatctctacc tagtctattg ttttgttata ggcttggttt atgatgttta
    16441 ctttttccat ctaataggga ttacaactct cttatttatc ttattgggag ccttccttca
    16501 taaattgaat agtgttattt tgttgaatcg ttggacaaga atgctagcta tgattgtact
    16561 gacattcctg tttgaaatgg gtagttatct tttggctttt atggtagggt tgacagtaga
    16621 tagcatgtcg atttttatag tctatagctt ggtaccgacg atgattttaa attttttatg
    16681 gattactgtt tttcaattta tttttgaaaa atattatcta taagaacgac atataaatgt
    16741 aacaaaggcg taatatttat taggcctttt tttggtatac tagtattgtc tttaaaagaa
    16801 ggagtatcta cgtaatatga agaaaaaaat cttagcgtca cttttattaa gtacagtaat
    16861 ggtttctcaa gtagctgttt taacaactgc gcatgcagaa acgactgatg acaaaattgc
    16921 tgctcaagat aataaaatta gtaacttaac agcacaacaa caagaagccc aaaaacaagt
    16981 tgaccaaatt caggagcaag tatcagctat tcaagctgag cagtctaact tgcaagctga
    17041 aaatgataga ttacaagcag aatctaagaa actcgagggt gagattacag aactttctaa
    17101 aaacattgtt tctcgtaacc aatcgttgga aaaacaagct cgtagtgctc aaacaaatgg
    17161 agccgtaact agctatatca ataccattgt aaactcaaaa tcaattacag aagctatttc
    17221 acgtgttgct gcaatgagtg aaatcgtatc tgcaaacaac aaaatgttag aacaacaaaa
    17281 ggcagataaa aaagctattt ctgaaaaaca agtagcaaat aatgatgcta tcaatactgt
    17341 aattgctaat caacaaaaat tggctgatga tgctcaagca ttgactacga aacaggcaga
    17401 actaaaagct gctgaattaa gtcttgctgc tgagaaagcg acagctgaag gggaaaaagc
    17461 aagtctatta gagcaaaaag cagcagctga ggcagaggct cgtgcagctg cggtggcaga
    17521 agcagcttat aaagaaaaac gagctagcca acaacaatca gtacttgctt cagcaaacac
    17581 taacttaaca gctcaagtgc aagcagtatc tgaatctgca gcagcacctg tccgtgcaaa
    17641 agttcgtcca acatacagta caaacgcttc aagttatcca attggagaat gtacatgggg
    17701 agtaaaaaca ttggcacctt gggctggaga ctactggggt aatggagcac agtgggctac
    17761 aagtgcagca gcagcaggtt tccgtacagg ttcaacacct caagttggag caattgcatg
    17821 ttggaatgat ggtggatatg gtcacgtagc ggttgttaca gctgttgaat caacaacacg
    17881 tatccaagta tcagaatcaa attatgcagg taatcgtaca attggaaatc accgtggatg
    17941 gttcaatcca acaacaactt ctgaaggttt tgttacatat atttatgcag attaatttac
    18001 agagggactc gaatagagcc ctcttttcag gttttaccgt gacaatccct attaaaaatt
    18061 atatcaaaat agcttgaaaa tattggaaaa gtatggtaaa atgaaaattg tcgtgtgaac
    18121 gataatactc attcttgatg aattgtgaag cagttgccct tgggtcgttt tgcgagttga
    18181 agtcaagaag aggaaaaaaa caaaaaggag aaatactcat ggcagtaatt tcaatgaaac
    18241 aacttcttga ggctggtgta cactttggtc accaaactcg tcgctggaat cctaagatgg
    18301 ctaagtacat ctttactgaa cgtaacggaa tccatgttat cgacttgcaa caaactgtaa
    18361 aatacgctga ccaagcatac gacttcatgc gtgatgcagc agctaacgat gcagttgtat
    18421 tgttcgttgg tactaagaaa caagcagctg atgcagttgc tgaagaagca gtacgttcag
    18481 gtcaatactt catcaaccac cgttggttgg gtggaactct tacaaactgg ggaacaatcc
    18541 aaaaacgtat cgctcgtttg aaagaaatta aacgtatgga agaagatgga actttcgaag
    18601 ttcttcctaa gaaagaagtt gcacttctta acaaacaacg tgcgcgtctt gaaaaattct
    18661 tgggcggtat cgaagatatg cctcgtatcc cagatgtgat gtacgtagtt gacccacata
    18721 aagagcaaat cgctgttaaa gaagctaaaa aattgggaat cccagttgta gcgatggttg
    18781 acaccaatac tgatccagat gatatcgatg taatcatccc agctaacgat gacgctatcc
    18841 gtgctgttaa attgatcaca gctaaattgg ctgacgctat tatcgaagga cgtcaaggtg
    18901 aggatgcagt agcagttgaa gcagaatttg cagcttcaga aactcaagca gattcaattg
    18961 aagaaatcgt tgaagttgta gaaggcgaca acgcttaatt tatacaaata gtaattacct
    19021 aggagggcgg ggcttagccc ggctctccta ttttcaaaaa atataggaga attaaaatgg
    19081 cagaaattac agctaaactt gtaaaagagt tgcgtgaaaa atctggtgcc ggtgttatgg
    19141 acgctaaaaa agcgcttgta gaaacagacg gtgacatcga aaaagcgatt gaattgcttc
    19201 gtgaaaaagg tatggctaag gcagctaaga aagctgaccg tgttgctgca gaaggtttga
    19261 ctggtgttta tgttaacggt aatgttgcag cagttattga agtaaacgct gaaactgact
    19321 tcgttgcaaa aaacgctcaa ttcgttgaat tggtaaatac tacagctaaa gtcattgctg
    19381 aaggaaaacc tgctaacaac gaagaagctc ttgctttgat aatgccttca ggtgaaactc
    19441 ttgaagctgc atacgtatct gcaacagcaa ctatcggaga gaaaatctca ttccgtcgct
    19501 ttgcattgat tgaaaaaaca gacgcacaac actttggagc ataccaacat aacggtggac
    19561 gtatcggtgt tatttcagtt gttgaaggtg gagacgaagc acttgctaaa caattgtcaa
    19621 tgcacatcgc agcgatgaaa ccaacagttc tttcttacaa agaattggat gagcaattcg
    19681 ttaaagatga gttggcacaa ttgaatcacg ttatcgacca agacaacgaa agccgtgcaa
    19741 tggttaataa accagctctt ccacacttga agtatggatc aaaagctcaa ttaactgatg
    19801 atgttattgc tcaagctgaa gctgacatca aagctgaatt ggctgcagaa ggcaaaccag
    19861 aaaaaatctg ggacaaaatt attccaggta aaatggatcg cttcatgctt gataacacta
    19921 aagttgacca agcttacaca cttcttgcac aagtttacat catggatgac agcaagacag
    19981 ttgaagcata ccttgaatca gttaacgctt cggtagttga gtttgctcgc tttgaagttg
    20041 gtgaaggtat cgagaaagct gcaaacgact tcgaagctga agttgcagct acaatggcag
    20101 cagccttgaa taactaataa tagaaaggaa gcaaattgct tccttttttg tatagggctt
    20161 agatttttcc tgaaattgtt ttttccccag tctcactcga ttagaataat taaaacaaaa
    20221 agccctataa taagggctaa gtgcatttag gagactatac ttcaaattca tagtaagcgc
    20281 g
//
LOCUS       DAVBHS010000020        20188 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVBHS010000020 DAVBHS010000000
VERSION     DAVBHS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20188
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.020"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(46..1458)
                     /gene="sufB"
                     /locus_tag="VQ651_000515"
     CDS             complement(46..1458)
                     /gene="sufB"
                     /locus_tag="VQ651_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET2753548.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(1508..1948)
                     /locus_tag="VQ651_000516"
     CDS             complement(1508..1948)
                     /locus_tag="VQ651_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET2753549.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            complement(1935..3161)
                     /locus_tag="VQ651_000517"
     CDS             complement(1935..3161)
                     /locus_tag="VQ651_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2753550.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPASARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            complement(3172..4434)
                     /gene="sufD"
                     /locus_tag="VQ651_000518"
     CDS             complement(3172..4434)
                     /gene="sufD"
                     /locus_tag="VQ651_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET2753551.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKINYLERLESRGEGSAKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSYADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            complement(4462..5232)
                     /gene="sufC"
                     /locus_tag="VQ651_000519"
     CDS             complement(4462..5232)
                     /gene="sufC"
                     /locus_tag="VQ651_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET2753552.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITRLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            complement(5382..5576)
                     /locus_tag="VQ651_000520"
     CDS             complement(5382..5576)
                     /locus_tag="VQ651_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HET2753553.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            complement(5604..7259)
                     /gene="dnaX"
                     /locus_tag="VQ651_000521"
     CDS             complement(5604..7259)
                     /gene="dnaX"
                     /locus_tag="VQ651_000521"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET2753554.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            complement(7259..7756)
                     /locus_tag="VQ651_000522"
     CDS             complement(7259..7756)
                     /locus_tag="VQ651_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET2753555.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            complement(7842..9044)
                     /gene="rpsA"
                     /locus_tag="VQ651_000523"
     CDS             complement(7842..9044)
                     /gene="rpsA"
                     /locus_tag="VQ651_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET2753556.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(9206..9277)
                     /locus_tag="VQ651_000524"
     tRNA            complement(9206..9277)
                     /locus_tag="VQ651_000524"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9243..9245),aa:Gln,seq:ttg)
     gene            complement(9287..9367)
                     /locus_tag="VQ651_000525"
     tRNA            complement(9287..9367)
                     /locus_tag="VQ651_000525"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9331..9333),aa:Tyr,seq:gta)
     gene            complement(9417..9647)
                     /locus_tag="VQ651_000526"
     CDS             complement(9417..9647)
                     /locus_tag="VQ651_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HET2753557.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(9815..10459)
                     /gene="pcp"
                     /locus_tag="VQ651_000527"
     CDS             complement(9815..10459)
                     /gene="pcp"
                     /locus_tag="VQ651_000527"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET2753558.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(10474..11397)
                     /locus_tag="VQ651_000528"
     CDS             complement(10474..11397)
                     /locus_tag="VQ651_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HET2753559.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(11394..12083)
                     /locus_tag="VQ651_000529"
     CDS             complement(11394..12083)
                     /locus_tag="VQ651_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HET2753560.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(12148..12852)
                     /locus_tag="VQ651_000530"
     CDS             complement(12148..12852)
                     /locus_tag="VQ651_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2753561.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSDM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGFPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(12908..13930)
                     /locus_tag="VQ651_000531"
     CDS             complement(12908..13930)
                     /locus_tag="VQ651_000531"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HET2753562.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(14062..16533)
                     /gene="parC"
                     /locus_tag="VQ651_000532"
     CDS             complement(14062..16533)
                     /gene="parC"
                     /locus_tag="VQ651_000532"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET2753563.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(16953..18896)
                     /gene="parE"
                     /locus_tag="VQ651_000533"
     CDS             complement(16953..18896)
                     /gene="parE"
                     /locus_tag="VQ651_000533"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HET2753564.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            19032..19673
                     /gene="plsY"
                     /locus_tag="VQ651_000534"
     CDS             19032..19673
                     /gene="plsY"
                     /locus_tag="VQ651_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET2753565.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(19718..20188)
                     /locus_tag="VQ651_000535"
                     /pseudo
     CDS             complement(19718..20188)
                     /locus_tag="VQ651_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         5793 a         4575 c         3647 g         6173 t
ORIGIN      
        1 gctgacgtgg tttgaagaga ttttcgaaga gtataaaatc aaattttatc caactgatcc
       61 ctccatttca tagctaatca agcggttcag ctcaactgcg tattccattg gaagttcttt
      121 tgtaaagggt tctacaaatc ccatgacaat catttcagtt gcctcagatt ctgacaatcc
      181 acggctcatg agataataca attgctcttc tgaaatctta gatactttgg cttcgtgttc
      241 caaagccact tgcgagttgt gaatttcatt aaatggaata gtatctgatg ctgacaagtc
      301 atccatgata atggtatcac attcaatgtg ggaaacagat ttcttagagt tcttgttaaa
      361 ggtgacttgt ccacggtagt caacctttcc tccaccttta gcgatggatt tagacacaat
      421 agacgagctg gtatgtggag cattgtgaat catcttagca cccgtgtctt ggtgttgccc
      481 tgcattagca aaggcgatag agagcatggt accacgcgct ccttctccat caagataaac
      541 agatggatat ttcatagtcg ttttggcacc caagtttcca tcaatccact caacagtggc
      601 atccttttga gccttagcac gctttgttac caagttatag acgttatcag accagttttg
      661 gatagttgta taacgcatat aagctccgtc caaagcaaaa atttctacaa tggcagcgtg
      721 caagctattg cttgaatatg ttggtgctgt acatccttct acgtagtgga cgcttgctcc
      781 ctcatcaacg ataatcaagg tacgttcgaa ctgacctata ttttcgttat tgatacggaa
      841 ataagtttga agtggaatat ctaccttgac accttttggc acgtagataa aagttccacc
      901 cgaccatact gctgagttga gggctgccaa cttgttatct gtcggcggta ccaacttcgc
      961 aaagtattgt ttaaataagt ctgggtattc cttgagtgcg gaatctgtat ctgtaaagat
     1021 aatacctaat ttttggaact cttccttcat gttgtggtaa accacttctg actcgtactg
     1081 ggcagaagcc cctgctaaat aagcacgttc agcttctgga atcccgatac gttcaaaggt
     1141 ttctttaatc ttttcaggta catcatccca agaacgggct ggtttgtcag atggtttttg
     1201 gtagtagatt aagtcatcaa agtcaatctc tgacaagtct gctccccaag tttgcatggg
     1261 catttttttg aaggtttcat aagacttcaa acggaactcc aacatccact caggctcacc
     1321 cttagcagca gataattcac gaataacacc ttcgttgagt ccttttcctg tcgataagac
     1381 aggctctaca tcgtcatgga aaccaaattt atattcacca aggtcaattg gttttggttc
     1441 tactctttct tcagccataa tatcctttct tcatttctaa taattcataa gacaaaagaa
     1501 acttgtctta ctgtttttct tgattttcaa ttgttttctt aagggcattc caagctaggg
     1561 ttgcacactt gattctttga gggaatttgg caacacctga caagaatgcc gcgtctccaa
     1621 gttggtcttg acgctcatct ttttgccctt gaaccatttc agaaaaaata gtcgccagtt
     1681 ctaaaatttc ttgtttggtt tttcctaaaa cggcatctgt catcatacta gcagaagcag
     1741 ttgaaatcgt gcatcctgaa tttagaaaag caatatcttc caaacggtcc tctgcatcaa
     1801 acttgacaga gaggttgatg acatccccac aagtcggatt gttgagactg atttgctcag
     1861 cgtcttctaa cttcccttgg tgatgtggat ttttcgaatg gtctgctacc actgccatat
     1921 aaaggctatc tagtttagaa agtgccattg aaaaactcct ttgtcttttg tagggcatcg
     1981 actagtttgt cgcaatctgc cttggtatta tagatataaa aacttgcacg agctgatgct
     2041 gggacttcca aatactgaag caagggttgc gcacaatggt gaccagcacg aacagccact
     2101 ccttcataat ccagagccgt cgcaagatcg tgaggatgga gatcacctag gttaaaggca
     2161 ataacacccg aacgttgagc caaatcctga gaaccgtaaa tggtcaatcc ctcaattgcc
     2221 tgcagttttg gatagacgta cgcaatcaat tcctgttcat gagcttcaac ggcatccata
     2281 ccaatctttt ccagataatc aactgcagtc gcaagtccaa tagctcctgc catatttggc
     2341 gttccagcct caaatttcca aggcaattcc ttccaactag cggattgctc gtagacaaaa
     2401 tcaatcatct cgccgccaaa ttctactgga gacatttgct caagatactt ttctttgccg
     2461 taaaggacac cgataccagt cggaccagcc atcttgtgac ccgaaaaggc gaaaaagtcc
     2521 agatccaagt cctggacatc aatcttcata tgaggtgtag attgagcacc atccactacc
     2581 atgattgccc caacttggtg ggctaattga gtgatttcct tgatcggatt gaccacacca
     2641 agaacattgg aggcatgagc tagggaaaca aatttaacct tatcagtcaa tttagctcgc
     2701 aaatcctcca tatccaaggc accgtcttta agatagacat agacaagctc tgctccagtc
     2761 tttcgacaag cttcctgcca tggaatgata ttagaatggt gttccattac tgaaatcaag
     2821 acctggtctc cctcagtgag aatttcctca gcaaagcgtg ccacccagtt aaggctggtt
     2881 gtcgttcctc tggtaaagag aacttccttt gtagagcctg cattaataaa cttacgaatg
     2941 gtttcacgag cagcttcata agaagctgtc gctcgttccg ctaaggtatg gacaccacgg
     3001 tgaacattgg cattgtcctg ctcatagtaa ctgttaatag ctttcagaac tactagtggt
     3061 ttttgtgtcg tcgcagcatt gtccagatag accagaggtt catcattgac aatctgatct
     3121 aaaattggaa aatccttgcg aatcgcttct acatctaaca taggcttccc cttagcgttt
     3181 tgacaatttc tcttcgatag ttgcaatcat ttcatcacga acttccttga ctggaatctc
     3241 cacgataaca gatccaagga aaccacgaac aaccaaacgc tctgcagttg ccttatccaa
     3301 tccacgactc atgaggtaat acatgtcttc tggatcaact tgtccgatag acgctgcgtg
     3361 tcctgcagtg acatcatttt catcaatcaa aagaattggg ttagcatctg aacgcgcttg
     3421 gtctgaaagc atgagaacac ggctctcttg ttgggcatct gctcccttag cacccttgat
     3481 gatgtggccg ataccattga aagtcaaagt tgctttttca aggataaccc catgttgtag
     3541 gatatttccg atagagttgc agccatagtt agttacacga gtatcaatcc cttgtacctg
     3601 acgaccactt gaaagagcta caaccttgag gtcagcatag ctaccattac caatcaagtc
     3661 actatcaaaa tcagcaacga catttccttc gttcatgaca ccgatagccc agtcaatact
     3721 tgcatcgttg cctaatttac cacgacggct aatgtaggca gtgacgtttt cacctagacg
     3781 gtcgatagca gcaaacttga cttgcgcacc tgaacgtgca atcacttcta ctgtgatatt
     3841 agcagttgct ttggcacttc cttcaccgcg tgactctaaa cgctccagat aattaatctt
     3901 agaattttta ccagcgataa tcataatatg cttgttaaac ggcacattgc tatcgctatc
     3961 ttggtagaaa attccttcaa ttggctctgt gatttctacg ttatctggaa tatagagtac
     4021 agcaccactg ttaaagtaag ctgtgtggta agccgccaac ttgtcatcat catacttaac
     4081 agatgacatg aagaattctt cgatcagctc tggaatttct tctaaagctg agtgaaagtc
     4141 tgtgaagaca acaccctgtt cagctaactc aactggagtt tgttcgaaaa cagtttgagt
     4201 tcctacttgc accaacttca agtgatgatc taaagctgtg aaatctggaa catttgctga
     4261 tggctcattt tctgtaatcg ttccatcacc cagattccaa cggtggaatt tgacacgctc
     4321 aataactggt aattccaaag tctcaatctt gtcaaaagct ttttgacgga gatcagccaa
     4381 ccagcttggt tcagcgtgca tttctgaaaa aagtttaata ttttctctag tcatttactt
     4441 ctcctaaaag atacgaggaa attacaattc ttccttgtag tcgtagccaa gttcttcagc
     4501 taattttgcg tatccttcac gttccaaacg cgcagccaat tctggaccac cagaaaggac
     4561 aacacgacct tccatcatca cgtgtaccac atcaggtgtg atatagttca aaagacgttg
     4621 gtagtgagtg atgatcatag caccaaaacc ttcaccacgc atggcattga cacctttaga
     4681 cacaacttta agagcgtcaa tatcaagacc tgagtcaatc tcgtccaaaa gagcaaatgt
     4741 tggctccaac atcaaaagtt gaagaatttc attgcgtttt ttctcaccac cagagaagcc
     4801 ttcgttgagg taacgctctg ccatttcttc tttcatgttg agcaattcca ttttttcatc
     4861 tagcctagta ataaactcac gaactgaaat cttctcatca tcttctttac ccgcattcat
     4921 agcggcacga agaaactcag cattggtaat tccagggatt tctgatgggt attgcatagc
     4981 aaggaaaagt cccatacgcg cacgctcatc cacttccaac tcaaggatgt ttacgccatc
     5041 aaacaaaact tcacctttag ttacttcata gtttggattt cccatgatag cggcagaaag
     5101 agtcgattta cctgtaccat ttggtcccat gatagcggca atttctcctg ttttcagggt
     5161 caggttaacc ccttttaaaa tttcttttcc ttcaatctca acgtgaagat ctttgatctc
     5221 taatactgac atgatagttc ctttcttttt caaaactttt acagtactta ctagaaaaac
     5281 gtctgatttc cccagaaata gagcaaaaat tctataaata tacttttcta gtataacaaa
     5341 aaaaagccta aaaggctttg attttgtctg gaagctgagg gctagtcttt cctattaaaa
     5401 tgctgatcga tgacatcaac gattttgccc atcacataac tgactcggaa atttctaaag
     5461 agtaaaattg cccaaaaggc tgccataata tagcggccag tcatccaggc ttcattgaaa
     5521 aaataggtct cagcagctgt aaacagcgcc ataataataa attgttgttt attcataaac
     5581 ttattgtatc acgaaatctt tctttagtct tcctctacct tcactttatc agccaaaaat
     5641 tcaaatcctt ctggaatcag gctttcttct acttcttttt cagtttgaga agatttggct
     5701 ttggctgaaa aggctgcgcg aacttctttc cattcctcca tggaaatagc taaaatctca
     5761 ggtgaaaaac ctgccgcctg actgaggata ttgccaaaca tggtattgag attgtctcgt
     5821 ttcatagttt gaccagcatt gaagttagac tcaaaagcaa gaatagcatg gtgttcattg
     5881 gcagcaaccg gttgagaacc aactagcaga gccttgtccg gcccacctag actttcaatt
     5941 acctctcccc aggcattctg caaacgaatt agattttgac gtgctaaatc aggattttcg
     6001 acggcctctt gtaagataga ttgcacttta ttgcgatcga cacgatagac tgttttgccc
     6061 gtagctggtc gactaggagc tggtgcaact tgtttaggaa ccgcacctac attagaaagc
     6121 tcttgtttga gacgggcaac ttcctgtctc agcgtagcaa tttcattttc aaccgctcct
     6181 gatagagctg gttcgggctt gatttccgcc aaacggacgg tcatcatttc agcataaatc
     6241 ttgggctgca aactagactt aatatctgct aaactcactg ttgctaagcg aatcatttca
     6301 aacagatttt tttgaggaag tgccaaattt tctacaaaga ctgaactatg atgagtattt
     6361 tctccccctg tttgaacaat taacaagtct cttaaatagt gcaaaagatc ggtcacaaaa
     6421 cgagtcatgc tcttaccatt gtcaaaaaga agattcaagc aagacaaagc tttgggaaca
     6481 tcctgttgag acaaggccgc cacataatca tccaaggctg ataggctaat agtgccagta
     6541 atttcttcag agatagcagt cgtcagctca tttccctgtg tcaaactcag ggcttgatcc
     6601 aaaatagaca aggcgtcccg cattccacct tccgcccgtc tggcaatgat ttccacagcc
     6661 tctggttcag aactgatatt ttctttttct aagatatagt gaatatgttc cttaatatcc
     6721 tgtgtcttaa ttgatttaaa ctcaaaacgt tgcacacggg atagaatagt agcaggaatc
     6781 ttgtgtaatt cagtagtggc caaaataaag actacattct gtgttggttc ttccagcgtc
     6841 tttaggaggg cattaaaagc ccctgtagac agcatgtgaa cctcatctat gatataaacc
     6901 ttataacgag caaggctagg cgcataggta gatttatcac gaatttcgcg aatttcatct
     6961 accccattat tagaagctgc atccatttca atgacatctt ctaaactacc gtccgtcact
     7021 gcttgacaaa tatagcagtt attgcaaggt tcgccaccca cttgattggg acagttcata
     7081 gccttggcaa agattttagc aacactggtt tttcccgttc cacgaggacc agaaaaaaga
     7141 taagcgtgac ttattttctc ttgctccacc gcttgtttaa gagtcttagc cacaacttct
     7201 tgaccaacta actgggagaa gttttgactt ctatattttc gataaagtgc ttgatacatt
     7261 aagatttttc ctcaaacatc gtaaagtccc atgttgtctt ttcaagcaaa atagcgacaa
     7321 attgttccaa ataatctcga tccatagcat cgtaatcctc aatctctgaa gaatccagat
     7381 ccagaactcc aagtaactga ccattcttca tcatcggcac cacaatttca cttttagcta
     7441 gactatcaca agaaatatag ttgagatagg tcgtcacatc tccaacaata acagtttcct
     7501 gaaagtgagc tgcctcacca caaacaccct tgcctagtgc aatacggatg caggaaacac
     7561 ctccttggaa gggacctaaa accaattcct ttccatcgaa caaataaaag cctgcaaata
     7621 cggtattagg aaaacgtgat tttataagag cactggcgtt ggaaagatta gccaaaacat
     7681 tggtttcgcc ttccaatagg aaggacaatt cttcatttaa catttgataa cgtgattgtt
     7741 tttctgattt taacatagaa ctattatatc aaaaaaggag cagagacaaa agaaaaatcc
     7801 cttgtttagt aaacaaagga ttttgtgaat tttcaatttg attaaagttc gatatcacca
     7861 aacaaatcag ccattgaaaa tcctgtttgt gtttctggaa gttcgaaatc acgcttttct
     7921 tgacgtcttg gacgacgtgg acgagcagca cgtttttctt ctttttgtcc ttcttcttgg
     7981 gctggacgtt cttcaagagc tttaatagaa agtgacacgc gttctgcatc tgcgttaact
     8041 tcaagaactt taacttgaac ttcttgacca actttaagag cttcttttgg attttcaatc
     8101 cgtttgtgtg aaatttgtga tacgtgaaca agtccatcga tacctggcaa tacttcaaca
     8161 aatgcaccga agtcagtcaa acgtttaact gttccttcta ctacatcacc tttagccaat
     8221 ttttgctcaa cgccatccca tggtcctggt actgttgctt taagtgaaag tgatacacgt
     8281 ccttcttctt cgttaagatc aaggattttc acttcaattt cttcaccaac agttacaact
     8341 gattttggtg atacgttacg ttcatgtgac aattcagtca agtgaaccaa tccgtcaaca
     8401 ccaccaaggt cgatgaaagc gccgaagctt gtgatacgag caactttacc agttacaaca
     8461 tcaccaacag ccaatttacc gaatacttca gcgcgagctg ctgcagtagc tgcttcaaca
     8521 acttcacgac gtgaaaggat gaagcggttt tctttagcgt taacttcttt gattttagta
     8581 tcaaattctt gacctacaaa acgctcagcg ttacgtacga aacgagtatc caacattgaa
     8641 gctgggataa atccacgaac accttcaaat tctactgaaa gtccaccttt aacggcacgc
     8701 gttcctttaa cagtaacaac ttcttcttcg cgaccaacaa gtttgtccca tgctttgcga
     8761 gcttcaaggc gttttttaga tacaaggtat gtaactgtat cagtatcttt accaactact
     8821 tgacgaagta caagaacatc caatacttct cctactttaa caaagtcatt gatatctgca
     8881 tcacgatcgt ttgtcaattc gcgaagagtc aagacacctt caacaccagt tccagagatt
     8941 gcaacgttag cttgagtcgc atcaactgtc aatacttcag cactaacaac atcaccagtc
     9001 tcaacttgac taacgctatt tagcaaatct tcaaattcgt tcatctaaaa aatcctccaa
     9061 caatcaagtt tttcttaaac ttgacatact tataattttt ttcctaagca cccgcaagga
     9121 catgttcata tcattcacgt ctttcaatta taaaaataaa ataccctaag atattttgct
     9181 ttttatcttg attattacct aagaactggg gtagctggat tcgaaccaac gcatgaggga
     9241 gtcaaagtcc cttgccttac cgcttggcta taccccatta atgaaatgga gagagaggga
     9301 ttcgaacccc cgaacccgaa ggagcggatt tacagtccgc cgcgtttagc ctcttcgcta
     9361 tctctcctac aatcaacatg gactattata tcatgaaaat ttcaaaaaaa caagacttat
     9421 tttgctaaat tataattttc aatcaaaatt tccacagctt tttccaattt tttataaaaa
     9481 ctatcgacat tcccattctt tataatggca aattgtccgt ctaattcggc aatttctccg
     9541 ataacttttt taccgatagt caatgtataa ccttcaaaac tgtctttacc aacattaact
     9601 ttggcatccg ctacttgaat ttcgattttt ttatctttct tgctcatttc atacctctct
     9661 tcactttttc tattttacaa gaaaatccta aaactagcaa gaaaaaactc tatatcaggc
     9721 ttctctgata tagagtttta tgtaaacaac ctcaattgat ttagcatagt taatagttag
     9781 taaacaagca taattgacta tcctttttag gttatcagtg agtctcaccg cctactaact
     9841 gcaaatcctt atctttgtag tctacaatag cttcaatagc tacctctatg cctcttacaa
     9901 tatcacatag actcattcct gcagtattcg gcttatttac tacttgttcc atcatatatg
     9961 gaatatgcat gaaacctgct ctcatattag ggaatttttt atccactaaa taaagagctt
    10021 ggtacatcaa atgattgcaa acaaaggttc ctgcactatt ggatacaact gccggaagtc
    10081 cctgtttttt gatagcttgt accatcgctt tgataggtaa actactaaaa taggccgatg
    10141 ctccatcaat acgaatcggt gtatcaattg gttgattgcc ttcgttatca ggtatgcgag
    10201 catcatcttg attaatagcc actcgttcag gtgttaagcc ggtcctgccg cctgcttgtc
    10261 caatacaaag tacagcatct ggttgatatc gtaatatttc tgcctctaaa acttctgacg
    10321 acttataaaa aaccgttgga atttctaccc agcgaacttc agccccatta atctcagatg
    10381 gtaataattt tacagcctcc aaagctggat taatcttttc acctccaaaa ggattaaaac
    10441 ctgtaaccaa tattttcatt ttattttcct ttactaaaat gcgagaaagt acattaagaa
    10501 tatgtgaata acaatcatta ctagagcaac acctgcttga gcctttataa cgccattctg
    10561 atctttcata tccatcaatg ctgctggtag agcgttaaaa ttagcagcca ttggggtcaa
    10621 taaggtccca caataacctg ctgtcatggc aagagcacca gccacaattg gattagctcc
    10681 cagagcaaat acaaagggaa ctccaacacc tgctgtaata acggtgaatg ctgcaaaagc
    10741 atttcccata atcattgtga atagaaccat tccaagaaca taagccaaaa ctcctataaa
    10801 gcgactatct gaaggaacaa taccgctaat cagatgagag ataacatcac caacacctgc
    10861 tacagtaaaa atagccccca aagcccctaa taattgagga acaatcccac ttgttgaaac
    10921 ttgctgagtc attcgattat tttctgataa cagactctta gggtgactat tggtaatcac
    10981 aagaacagaa attgtagcaa acaaggcggc aaggctaatc gaaatcttgc taaattctgg
    11041 aatcatttgc gctaagacca acgcaagtat tgccatcagc ataactggaa taaaaatttt
    11101 atttttcaac ctgttagatt caatattggc tttcatttca tctaaggatg gcaaggttcc
    11161 gatacggact tgcttaaaca atgttaacag cgataatagg attacaataa taccaatact
    11221 catatttggc atataggaac cacctataaa cgtaatagac aatagagtcc aaaatgcaga
    11281 tgtcccaagt cgaactgggt ttgttttatc tttataacta caataggctg tatggagaaa
    11341 ttgacaacca atcacaatat aggtcaactc taatagttgc tttgccaact ctgtcatttt
    11401 tgttctcctc ccctagtctt ttttgatatc aattttttat caaataaata attgtaaatc
    11461 cccactacaa taagtgttat aacagcaaca ataatagatg tagaagcaat ccctgcataa
    11521 ttgctttcat agcctaactg atctaatgtt ccccctatca agaggactcc cccagcacct
    11581 acaaacgtat tttgagcaaa gaaatttcca aaattttcat tcgcagccgc acgcgctttt
    11641 attgtctcat cttcaacctc tgttaacttt ctacctaatt gagactctgc agctgcttct
    11701 cccataggtt gaaccaaagg tctgacaaac tgagggtgtc ctcctagacg aattgaaaag
    11761 aaaccagcta actctcgaat aaagaaataa actgtataga agtttccaac tgtcagacct
    11821 ttaatctttc gaatcaaatc gattgatcgt tgcttgagtc caaaggtttc tgacagcccc
    11881 acaagaggca aggtaaccat aaaaatcgtg agcactcgct gattgctaaa ttcttttccc
    11941 aaaatctcca aaaattcaac gagagaaaca cctgaaacta aagctgtaac caaaccagct
    12001 aagactactg ttgcaattgt atcaaatttt aaaataaaac ccacaacaat gattgctatt
    12061 cctattaatc taatccactc catatcaaac tcctttatat tcaaaatgac agtattttaa
    12121 aaattttatc aagatcaata ccattcctta tttaatgtat ttttctagtt ctttttggta
    12181 tttgctattg gattccaatt tttctttttg ccatttttta aaaacctcgt tatattcttt
    12241 tgttgtaaca atatcttttt gcaatttcat tcctttaaag atatatggtt cccccttaat
    12301 accaacttgt gagtatggtt ttgagaatgg tactacgtta cttacaactg gaaaaccacc
    12361 agatgaagct gttggcatca ataatgaact atctgtcgac caagcttgag ctttggcata
    12421 tttttcatat ctcttctcta ggtcagtggt ctcagaaaca gcatcttcta acaatttctt
    12481 atatttatcc aaaccaggtt tagctacaac atccttatct tttcctttcg taatgccaag
    12541 gtgtttcatg tcagaaccag attttggatc cataatattc aagtaagacg ctggatcttg
    12601 atagcttgga gcccatcctg tactgttcaa atcatagtct ttttgagaag gagcaacatt
    12661 gccgtattta tcattttcca tcaaaccatc aataacattt ccaataacgt ctgtcctcga
    12721 tgttcgagtc gctatactgt agcccaatga tgctggatct actgcataga cataagaaaa
    12781 tgttgtcggt gcatctgctt ctttatcagt ttttccacaa gccactaaaa tagctgacgt
    12841 gctcaggacc actcctgctg ttaagagcca ctttttctat ttcataaaaa atctcctttg
    12901 gtttatttta atctactttt acaatccaac cttctggcgc ttcaatatcg ccaaactgaa
    12961 tacccgtcaa ttcattatat aatttacgcg tcacaggacc tacttctgtt tcactataga
    13021 atacatggaa atcatcacca tgttgaatac ctccaattgg agaaataacc gctgctgtac
    13081 cacaggcacc tgcctctaca aaacggtcaa gattatcaat tggaacatca ccctcaatag
    13141 gagttaatcc caagcgatgt tctgccaaat aaagcaagga atacttggta atagatggca
    13201 agatagatgg actcaatggt gttacaaatt cattatcagc tgtaattcca aagaagttag
    13261 ctgatccgac ttcttcaatc tttgtatgag ttgatgggtc cagatagata acatctgaga
    13321 aatgacgtga cttggccatt tttcctggta agagacttgc agcatagttt ccaccaacct
    13381 tagccgcacc tgtaccattt ggtgctgcac ggtcgtactc atcctgaatc aagaagttgg
    13441 ttgggaccaa accaccttta aagtaatttc caactggcat agcaaagatg gtgaaaatgt
    13501 actcttctgc cggttttacc ccgataatat ctccgacacc aatcaaaaga gggcgaagat
    13561 ataaggttcc acctgttccg tatggtggta cgtattcttc attcgcacgg acaactgctt
    13621 tacaagcttc tacaaacatg tctgtcggaa cttgtggcat caagagacgg tcacatgtac
    13681 gttgcagacg tttagcattt tcatcaggac ggaacagttg aacactgcca tccttagtac
    13741 gataagcttt caaaccttca aatgcttgtt gtccatagtg aagacttgga gaagactctg
    13801 aaatatgcaa agttgcatcc tctgtaagct ctccttgatc ccattgtcca tttttgaaat
    13861 gagcaagata gcgataaggt aatttcatat aggaaaaacc gaggttttcc caatcaatcg
    13921 ttactgtcat attccactcc ttattctaaa aacctatttc ttatattcta cactattttt
    13981 ctaaaatagc aagtatattt tgtaattttc agaaaatttc tccaataaaa accagctctt
    14041 agaactgatt cttcatttca cttatttatc ttcagtaact acttcctgaa gataagcgtc
    14101 aaaaacttct tcatctgaaa tcgtgtcaga aatgaagctt ccattgctag tgcgttctga
    14161 caagttcaag tcttgcaatc ggctttcata gattgttcct ttattggatt ggacaagcag
    14221 agtttggtcg ttcacatcca cttccgtact gaagaaatcg ccaacaaatc cttgctctgc
    14281 aactgctcct gccaagaaga cacgatgcgg tttgtttttc aactcacgca agacttgtaa
    14341 tcctcgtttg gcacggctgg ttgctagaat ttcctcaata gaaacacgtt tcaagcttcc
    14401 acgctgggtc aagaggtaga aggacgaagt attacagata aagccagatt ggaggacatc
    14461 atcttctttc aaattcatag ccttgacacc tgctgcctta gcaccgacaa ccggaacctc
    14521 ttcgatattg aaacgcaggg cataaccatt ttgactaacc aagacaacat catctagttt
    14581 aatcggagcc actgctacaa tctgatctgt atcgtctttg agcttagcat acttgacaga
    14641 cttagatcta taggtccgcc atggagtgaa ttcttttcgc tctacccgtt tgatttgacc
    14701 aaggcgagtc actgcaaagt aggttgtcgc atcgtcaaac tgatccagta cttccacata
    14761 aaggatttct tcattcgttt caaagtttgt gatggtttgg ctcagatgct ctccgatgtc
    14821 cttccaacga atatctgcca actcatggat tggtctgtag atgacatttc caagacttgt
    14881 gaacatcaag aggtgctggg ttgtcttggc agattgaaca aaaatcaaac ggtcatcatc
    14941 acgcttgcca atttcttcca aggtggaagc cgcaaaggaa cgtggactgg tacgcttgat
    15001 gtaacctgcc ttggtcacgc tgacgtaggt atcttcctca gcgataagac tagctgtatc
    15061 aatctcaatt gctttcgcag tgtcttctaa agaactcaaa cgaggagttg caaatttctt
    15121 cttgacctca cgaagttctt tcttcatgag attgtacata gtcctttcat caccgataat
    15181 agccgccagc atagcaatct tctcacgaag ctctgcttct tcttcctgca agacaaccac
    15241 atcggtattg gtcaaacggt acagttgcaa agttacgata gcctcagcct gttcttccgt
    15301 aaaatcatag ctaactttga ggttttcctt ggcgtccgcc ttattctcag aagcacggat
    15361 aagagcaatg acttcatcca aaatcgaaat cacacgaatc aaaccttcga cgatatggag
    15421 acgtttctca gccttttctt tgtcaaagcg tgaacgcgcc aaaatcactt ctcgacggtg
    15481 agcgatgtag ctagacagga ttggaacaat cccaacctga cgaggtgtga aattgtcaat
    15541 cgccaccata ttaaagttgt agttgatttg taggtcggtg tacttaaata agtagttgag
    15601 aacaagctca gtattagcgt ctttcttaag ttcgatagcg atacgaagac catcacggtc
    15661 agactcatca cgaacctcag caatcccagc taccttgtta ttaacacgaa catcatcgat
    15721 tttcttgact agattggcct tattgatttc ataaggaatc tcagtaataa cgatttgttc
    15781 cttaccacct tttagctttt caatttcagt cttggaacga acaaccacgc gccctttccc
    15841 agtctcataa gctttcttga tttcatcacg accctgaata atagcccctg tagggaagtc
    15901 tggtccaggc aagaattcca tgagtttatc aatctttgca gttgggtggt caatcatgta
    15961 aactgcagca tctatgacct cagctaaatt atggggagga atgtctgtgg cataaccagc
    16021 cgaaatccca gtcgaaccat tgaccaagag gtttggaaag gctgctggca agaccgttgg
    16081 ttctttctcc gtatcgtcaa agttccatgc aaaaggaact gtatttttct cgatatcctg
    16141 aagaaggtaa cctgcaattt cagacaaacg tgcctcagta taacgcatag ccgcaggagg
    16201 atctccgtcc atagaaccgt tattaccgtg catttcaact agaatctcac gatttttcca
    16261 gttctgtgac atacgaacca tggcatcata gatagaagaa tccccgtgtg ggtggaaatt
    16321 ccccatgatg ttcccgactg acttggccga cttacggtag ctcttgtcaa aagtattgct
    16381 atccttattc atagaataaa gaatacggcg ctgaaccggc ttcaacccat cacgaatatc
    16441 tggcaaagcc cggtcttgaa taatgtactt ggagtagcga ccaaagcgct ctcccatgat
    16501 gtcctccagg gacatgtttt gaatgttaga cataagatac aaagcccata aaataccaag
    16561 tgaaaataga aaattcttga agtaagcaaa ctcacaagag aatttatctt tttcacacag
    16621 tatctagggc gtgttcaact cctttcaaag aatgtagagt aggtttttat gcagtaaaag
    16681 atattttacg ggaattcctc ccgtgttcag ttacgataag taaccaaact atcctgtttg
    16741 tatttttcaa tatgaaaatc tggttttcca aaattagtct tagtttgtgt cttagccgct
    16801 cccttaagcg cctctttgag ataagcactc atagcagatt cttcattaat aatcctgcaa
    16861 ttttttcaaa ccaagatttt caaactgctt tttcacatag tcattcacat ccgactctaa
    16921 tttccagttt actaacatat tattttcttt cattaaaaca ctgtcgcttc ttctagcgta
    16981 aacttgacat tatcttcaat ccatttacgg cgtggttcta ccttatctcc catgagaaca
    17041 ttgacgcggc gttcggcgcg cgctaaatct tcaattgtga cacggatgag ggtacgtgtt
    17101 tctgggttca tggttgtttc ccagagctgg tccgcattca tctcaccaag tcctttgtat
    17161 cgttggaggg tagcgccttt accgaactgt ttacggagtt cttctagttc tccgtccgtc
    17221 caagcgtagg ccacttcttc tttctttcct ttacctttgg acatcttgta aagaggtggg
    17281 agggcaatat agacatgacc tgcctcgact agcggacgca tgtaacggta gaaaaatgtc
    17341 aagagcaagg tctggatatg ggcaccgtcg gtatccgcat cggtcatgat aatgatctta
    17401 tcatagttgg catcttcaat agagaagtct gctccaacac ccgcaccaat ggtataaatc
    17461 atggtattga tctcttcatt tttgaggata tccgccatct tggccttggc tgtattgata
    17521 accttaccac gaagaggtag aatagcctgg aacttgcggt cacgaccttg tttggcagaa
    17581 ccaccggcag agtccccctc aactagatag agttcattct tagcaggatt cttagattgg
    17641 gctggggtca atttcccaga caacaagccc ttatctttct tgtttttctt cccatttcgg
    17701 ctctcatcac gcgccttacg tgctgcttca cgagcatcac gggccttgat agccttgcgg
    17761 atgaggttag aagctaattc cccattttcc ataaggaaaa aggtcaactt atcagccact
    17821 attccatcca caactgggcg agctaggggg cttcctagtt tatccttggt ctgtccttca
    17881 aactgcaagt gttcttcagg aactaagata gaaagaacgg ccgctagtcc ctcacgatag
    17941 tctgaacctt caaggttttt atctttttcc ttgagaagac ctgttttacg tgcatagtca
    18001 ttcatgacct tggtaatggc agacttgagt cctgtctcgt gcgttccacc gtccttggtg
    18061 cgaacgttat tgacaaagga tagaatgtta tctgagaatc cgtcattgta ctggagggct
    18121 acttccactt gaaaaccatt gtcttcccct tcaaagtaaa gaactggcgt caagatttcc
    18181 ttatcttcgt tgagataaga aacaaaatct tgtactccat tctcatagtg gaactcaatc
    18241 gcttcatctg ttcgcttgtc cgttaaagac aaggtcacat ttttcaagag aaaggctgat
    18301 tcattaaggc gctctgaaat ggtattgtac ttgaaatctg tcgtagaaaa tatagtcgcg
    18361 tcaggcataa aagtaacttt ggtgcctgtt ttagacttgg gtgctgtacc gattttcttc
    18421 aaagtcgtga caggttttcc accattttcg aaacgttgct tgtaaactgc gccatcacgg
    18481 gtaatttcaa cttctaacca gctagaaagg gcgttaacaa cggaagaacc cactccgtga
    18541 agtccacctg atgtcttata gccaccttga ccgaatttcc ctccggcatg aagaatggta
    18601 aagataacct caacagttgg aattcccata gcgtgcatac ctgtcggcat cccacgtcca
    18661 tggtcttgaa ccgttagact accgtcttta ttgatggtta catcgatacg atcaccaaac
    18721 ccagacaagg cttcatcgac tgcattatca acgatttccc aaactaggtg atgaagacca
    18781 gcgccatcgg tcgatccaat atacatccct ggacgttttc ggaccgcatc caacccttct
    18841 agcacctgaa tagcatcatc attataattg ttaatattga tttccttttt tgacacaagg
    18901 aacctcctat tcgttcatct ttactattct acaggttttc caaggatttt gcaaaatttt
    18961 tctttctccg atgtgacaat tttagcagag attctctgct tttctttccc aattcatgat
    19021 ataataggag tatgattaca atagttttat taatcctagc ctatctgctg ggttcgattc
    19081 catctggtct ctggattgga caagtattct ttcaaatcaa tctacgcgag catggttctg
    19141 gtaacactgg aacgaccaat accttccgca ttttaggtaa gaaagctggt atggcaacct
    19201 ttgtgattga ctttttcaaa ggaaccctag caacgctgct tccgattatt tttcatctac
    19261 aaggcgtttc tcctctcatc tttggacttt tggctgttat cggccatacc ttccctatct
    19321 ttgcaggatt taaaggtggt aaggctgtcg caaccagtgc tggagtaatt ttcggatttg
    19381 cgcctatctt ctgtctctac cttgcgatta tcttctttgg agctctctat cttggcagta
    19441 tgatttcact gtctagtgtc acagcatcga tcgcggctgt tatcggggtt ctgctctttc
    19501 cactttttgg ttttatcctg agtaactatg actctctctt catcgctatt atcttagcac
    19561 ttgctagttt gattatcatt cgtcataagg acaatatagc tcgtatcaaa aataaaactg
    19621 aaaatttggt cccttgggga ttgaacctaa cccatcaaga tcctaaaaaa taaaatgcca
    19681 gttctgtgaa ctgcacccca aaagttagac aattaattta tccgaaggat ttagttctgt
    19741 attgcacagg gctaagtcct tttagtttta tcttaattcg tttattgttg tagtaatcaa
    19801 tatagtctat aatggcttgt tccaattgct taagcgactg aaacgacttc tcataaccgt
    19861 aaaacatttc cgatttcaga atcccaaaga aggactccat cataccattg tctgggctgt
    19921 ttcccttacg tgacatggat gcttgaattc ccttactctc taggaactga tgataagaat
    19981 cgtgttgata ttgccagcct tggtcactat ggagaatcgt attctcgtag tgcttctctt
    20041 tgaatgcctg ttccaacatt gtttatactt gttctaagtt gggcgaagtt gaaagattat
    20101 aggcgataat ttcgctgtta aagccatcta aaactggtga taagtaaagc ttttgagtac
    20161 ttgctggaat ggcaaattct gtcacatc
//
LOCUS       DAVBHS010000021        19966 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVBHS010000021 DAVBHS010000000
VERSION     DAVBHS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19966
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.021"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            380..1333
                     /locus_tag="VQ651_000536"
     CDS             380..1333
                     /locus_tag="VQ651_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2753566.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLISLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            1384..2574
                     /locus_tag="VQ651_000537"
     CDS             1384..2574
                     /locus_tag="VQ651_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET2753567.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGTDAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            2723..3094
                     /gene="rnpA"
                     /locus_tag="VQ651_000538"
     CDS             2723..3094
                     /gene="rnpA"
                     /locus_tag="VQ651_000538"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET2753568.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            3069..3893
                     /locus_tag="VQ651_000539"
     CDS             3069..3893
                     /locus_tag="VQ651_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET2753569.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAKIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            3912..4898
                     /locus_tag="VQ651_000540"
     CDS             3912..4898
                     /locus_tag="VQ651_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HET2753570.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            4949..5572
                     /locus_tag="VQ651_000541"
     CDS             4949..5572
                     /locus_tag="VQ651_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HET2753571.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPGWQLQQTSQWMNQAQRLRPHFWVYLQRDEQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            5703..7160
                     /locus_tag="VQ651_000542"
     CDS             5703..7160
                     /locus_tag="VQ651_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2753572.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            7183..7464
                     /locus_tag="VQ651_000543"
     CDS             7183..7464
                     /locus_tag="VQ651_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2753573.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            7541..8026
                     /locus_tag="VQ651_000544"
     CDS             7541..8026
                     /locus_tag="VQ651_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2753574.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            8043..8708
                     /locus_tag="VQ651_000545"
     CDS             8043..8708
                     /locus_tag="VQ651_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HET2753575.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            8712..9575
                     /locus_tag="VQ651_000546"
     CDS             8712..9575
                     /locus_tag="VQ651_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HET2753576.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SRGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            9577..10281
                     /locus_tag="VQ651_000547"
     CDS             9577..10281
                     /locus_tag="VQ651_000547"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HET2753577.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVAWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            10460..12133
                     /locus_tag="VQ651_000548"
     CDS             10460..12133
                     /locus_tag="VQ651_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2753578.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            12245..13336
                     /gene="ulaG"
                     /locus_tag="VQ651_000549"
     CDS             12245..13336
                     /gene="ulaG"
                     /locus_tag="VQ651_000549"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HET2753579.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            13450..15426
                     /gene="tkt"
                     /locus_tag="VQ651_000550"
     CDS             13450..15426
                     /gene="tkt"
                     /locus_tag="VQ651_000550"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2753580.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVTDYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            15543..15842
                     /gene="yajC"
                     /locus_tag="VQ651_000551"
     CDS             15543..15842
                     /gene="yajC"
                     /locus_tag="VQ651_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET2753581.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVEGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            15889..16317
                     /locus_tag="VQ651_000552"
     CDS             15889..16317
                     /locus_tag="VQ651_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HET2753582.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            16319..16729
                     /locus_tag="VQ651_000553"
     CDS             16319..16729
                     /locus_tag="VQ651_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753583.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            17029..19680
                     /gene="adhE"
                     /locus_tag="VQ651_000554"
                     /gene_synonym="adhC"
     CDS             17029..19680
                     /gene="adhE"
                     /locus_tag="VQ651_000554"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HET2753584.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITEPSMEATSALMNHEGIATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT         6266 a         3575 c         4326 g         5799 t
ORIGIN      
        1 gtgcattggt tagttgttta ctttcttcat aaattttatt gtatcatatt tttgtttcac
       61 aggaagtcta ataaataaaa attctttaaa attttgttgg gaaaatggga aaagatataa
      121 atggaaactt agtataacac ttggaaaact ctgtaaaaat agtaagtcaa ttcatgtccc
      181 gttttttctc tagggaagtt gggcggctat atttatatga ccatttgtcg taataaacat
      241 ccctaccaag ggtcaataga aataaattca acgaaacttt tgctgtcaga tttagatgcc
      301 caatttgtca tttatactct ttcatatagg actttctata aggatggaaa aaagaaggga
      361 aatttgataa gataggaata tggattttga aaaaattgaa caagcttata cctatttact
      421 agagaatgtc caagtcatcc aaagtgattt ggcgaccaac ttttatgacg ccttggtgga
      481 gcaaaacagc atctatctgg atggtgaaac tgagctaaac caggtcaaag acaacaatca
      541 ggcccttaag cgtttagcac tacgcaaaga agaatggctc aagacctacc agtttctctt
      601 gatgaaggct gggcaaacag aacccttgca ggccaatcac cagtttacac cggatgctat
      661 tgctttgctt ttggtgttta ttgtggaaga gttgtttaaa gaggaggaaa ttactatcct
      721 cgaaatgggt tctgggatgg gaattctagg cgctactttc ttgatctcgc ttgataaaaa
      781 ggtggattac ttgggaatgg aagtggatga tttgctgatt gatctggcag ctagcatggc
      841 agatgtaatt ggtttgcagg ctggctttgt ccaaggagat gccgttcgtc cacaaatgct
      901 caaagaaagc gatgtggtca tcagtgactt gcctgttggc tattatcctg atgatgccgt
      961 tgcgtcgcgc catcaagttg cttctagcca agaacatact tacgcccatc acttgctcat
     1021 ggaacaaggg cttaagtacc tcaagtcaga cggatacgct atttttctag ctccgagtga
     1081 tttgttgacc agtcctcaaa gtgatttgtt aaaagaatgg ctgaaagaag aggcgagtct
     1141 ggttgctatg attagtctgc ctgaaaatct ctttgctaat gctaaacaat ctaagactat
     1201 ttttatccta cagaagaaaa gtgaaatagc agtggagcct tttgtttatc cacttgctag
     1261 cttgcaagat gcaagtgttt taatgaaatt taaagaaaat tttcaaaaat ggactcaagg
     1321 tactgaaata taaaatagat tttgttataa tagttgaaaa cgcttaaaaa ggggtatcat
     1381 gttatgacaa aaacaattgc aatcaatgca ggaagttcaa gtttgaaatg gcaattatac
     1441 ttaatgccag aggaaaaagt gttagccaaa ggtttgattg aacgtatcgg tttgaaagat
     1501 tcaatttcaa ctgtaaaatt tgacggccgt tctgaacaac aaattttgga tatcgaaaat
     1561 catacacaag ctgttaaaat tttattggat gacttgattc gtttcgatat tatcaaggct
     1621 tatgacgaga ttacaggtgt tggccaccgt gttgtagcag gtggagaata tttcaaagaa
     1681 tcaacagttg ttgagggaga tgttttagaa aaagttgaag agttgagttt gttggctcct
     1741 ctacacaacc cggccaatgc agcaggtgtt cgtgctttca aggaattgtt gccagacatt
     1801 accagtgtag ttgtttttga tacttccttc cacacaagta tgccagagaa agcttatcgc
     1861 taccctctac caacaaaata ttacacagaa aacaaggttc gtaaatacgg tgctcatggt
     1921 acaagtcacc agtttgtagc aggagaagct gcaaaactct tgggacgtcc attagaagac
     1981 ttgaagttaa ttacctgtca tattggtaac ggaggctcaa ttacagctgt gaaagccggc
     2041 aaatctgtag acacttctat ggggttcact cctcttggtg gtattatgat gggaacgcgt
     2101 acaggggata ttgatccagc tatcattcct tatttaatgc aatatacaga ggattttaac
     2161 acaccagaag atatcagtcg tgttcttaac cgtgaatcag gtcttttggg agtttctgct
     2221 aattctagcg atatgcgcga tatagaagca gctgtagcag aagggaatca cgaggctagc
     2281 ttggcttatg aaatgtatgt tgaccgtatc caaaaacata tcggtcagta ccttgcagtg
     2341 ctaaatggaa cagatgccat tgttttcaca gcaggtgtcg gtgaaaatgc agagaatttc
     2401 cgtcgtgatg taatctcagg gatttcgtgg tttggttgtg atgttgatga tgaaaagaat
     2461 gtctttggcg ttacaggaga catctcaaca gaggcagcta aaatccgtgt cttggttatt
     2521 ccaacagatg aagaattagt cattgcccgt gacgttgaac gcttgaaaaa ataagtgaaa
     2581 ctaaaaaaat attcaataca aggagttggg aaagttattt ttccagcttc tttttctgat
     2641 gaaattgtcc aaaaccttgc tatgattggc ttttttgaaa aatatggtat aatagtagta
     2701 atttaataga tggagttgag ttttgaagaa aaactttcgt gtaaaaagag agaaagattt
     2761 taaggcgatt ttcaaggagg ggacaagttt tgctaatcgc aaatttgtgg tctaccaatt
     2821 agaaaaccag aaaaaccatt ttcgagtagg tctatcagtt agcaaaaaac tggggaatgc
     2881 cgtcactaga aatcaaatta agcgacggat tcggcatatt atccagaatg caaaagggag
     2941 tctggtagaa gatgtcgact ttgttgtcat tgctcgaaaa ggagtcgaaa ccttgggata
     3001 cgcagagatg gagaaaaatc tactccatgt attaaaatta tcaaagattt accgggaagg
     3061 aaatgggagt gaaaaagaaa ctaaagttga ctagtttgct aggactgtct ctgttaatca
     3121 tgacagcctg tgcgactaat ggggtaacta gcgatattac agccgaatcg gctgattttt
     3181 ggagtaaatt ggtttacttc tttgcgaaaa tcattcgctt tttatcgttt gatattagta
     3241 tcggagtggg gattattctc tttacggtct tgattcgtac agtcctcttg ccagtctttc
     3301 aggtgcaaat ggtggcttct aggaaaatgc aggaagctca gccacgcatt aaggcgcttc
     3361 gagaacaata tccaggtcga gatatggaaa gcagaaccaa actagagcag gaaatgcgta
     3421 aagtatttaa agaaatgggt gtcagacagt cagattctct ttggccgatt ttgattcaga
     3481 tgccggttat tttggccttg ttccaagccc tatcaagagt tgacttttta aagacaggtc
     3541 atttcttatg gattaacctt ggtagtgtgg atacaaccct tgttcttccg attttagcag
     3601 cagtattcac ctttttaagt acttggttgt ccaacaaagc tttgtctgag cgaaatggcg
     3661 ctacgactgc gatgatgtat gggattccag tcttgatttt tatctttgca gtttatgcgc
     3721 caggtggagt cgccctatac tggacagtgt ctaatgctta tcaagtcttg caaacctatt
     3781 tcttgaataa tccattcaag attatcgcag agcgcgaggc cgtagtacag gcacaaaaag
     3841 atttggaaaa tagaaaaaga aaagccaaga aaaaggctca gaaaacgaaa taaataagga
     3901 ggaatctggt aatggtagta tttacaggtt caactgttga agaagcaatc cagaaaggat
     3961 tgaaagaatt agatattcca agaatgaagg ctcatatcaa agtcatttct agggagaaaa
     4021 aaggctttct tggtctattt ggtaaaaaac cagcccaagt ggatattgaa gcgattagtg
     4081 aaacgactgt tgtcaaagca aatcaacagg tagtaaaagg cgttccgaaa aaaatcaatg
     4141 atttgaacga gcctgtgaag acggttagtg aagaaaccgt tgaccttggt catgtggttg
     4201 atgctattaa aaaaatagag gaagaaggtc aaggtatttc tgatgaagtc aaggctgaaa
     4261 tcttaaaaca tgaaagacat gccagcacta tcttagaaga aactggtcac attgagattt
     4321 taaatgaact tcaaatcgag gaagcgatga gggaagaagc aggcgctgat gaccttgaaa
     4381 ctgagcaaga tcaaactgaa aatcaagact tgaaagagat gggcttgaag gtcgagcaaa
     4441 gttatgatat tgcccaggtg gctacggatg tgactgccta tgttcaagcg attgtggatg
     4501 acatggatgt tgaagctaca ctttcaaatg attataaccg tcgtagcatc aatctacaaa
     4561 ttgacaccaa cgaaccaggt cgtattatcg gctaccatgg taaagtcttg aaggccttgc
     4621 aactgttggc tcaaaattat ctttacaacc gctattccaa aaccttctac gttacaatca
     4681 atgtcaatga ttatgtcgaa caccgtgcag aagtcttgca gacctatgcg caaaaattgg
     4741 cgattcgtgt tttagaagaa ggacgtagtc atcaaacaga tccaatgtcg aatagtgaac
     4801 gcaagattat ccaccgtatt atttcacgta tggatggcgt gaccagttac tctgaaggtg
     4861 atgagccaaa tcgctatgtc gttgtagata cagaataagt aaaatcaggg ttgacctgat
     4921 tttttgctag ctagaggagg ttaaactaat gttgaataag ataagagact atttagactt
     4981 tgctggtttg cagtaccgta atcctgataa agcgggagca gagcgagaga agatgctggc
     5041 attccgccac aaaggacaag aggcccgaaa ggcttttaca gaactggcca aagcctttca
     5101 agcaagccat ccaggatggc aactccaaca gactagtcag tggatgaatc aggcccagcg
     5161 tttgagacca catttttggg tttatctaca gagagacgaa caagtgacag aacctatgat
     5221 ggccttacgt ttgtatggga catctactga ctttggaatt tctttggaag tcagtttcat
     5281 cgaacgtaag aaggatgagc aaacactggg caagcaggcc aaagttttag acattccaac
     5341 cgttaaaggg atttattatc taacctactc taatggtcaa agtcaacggt gggaggcgaa
     5401 tgaagaaaag cgtcgtactt tacgcgagaa ggtgagaagt caagaagttc gaaaagtttt
     5461 agtgaaggta gatgttccta tgacagaaaa ttcgtctgaa gaagaaatcg tagaaggctt
     5521 attgaagtct tattctaaaa ttcttcccta ttatctagct acgagaaaat aagataattt
     5581 gtaaaacatc ataaatcata cagtccaaga gtgaacagtc cgctgtgtaa ttcttggtct
     5641 ttttgtttgc gctttcgcat tatataataa acttacaaaa acaattcaaa aggagaacaa
     5701 ttatggaagt cgtttcaagt gttctaaatt ggttttctag caatattttg cagaatcccg
     5761 catttttcgt aggtttattg gtgttgatag gatatgcact tttgaaaaaa cctgcccatg
     5821 acgttttttc agggtttgtt aaagcaacag tagggtatat gttgcttaac gtgggtgctg
     5881 gtggtttggt tacaaccttt cgtccaatct tagcagctct taactacaaa ttccaaattg
     5941 gtgcagcggt tatcgaccct tactttggac ttgctgcagc aaacaacaaa attgcagcag
     6001 agtttccaga ttttgttgga actgcaacta cagctctatt gattggtttt ggaataaata
     6061 tcttgctcgt agctcttcga aagattacga aggtaagaac cctctttatt actggtcaca
     6121 tcatggtaca acaagctgca acagtatctc ttatggttct attcttagta ccacaattgc
     6181 gcaatgctta cggtacagca gcgattggta tcatctgtgg actttactgg gcagttagtt
     6241 caaatatgac tgttgaggca actcaacgct tgactggtgg tggcggattt gcgattggtc
     6301 accaacagca atttgcaatc tggtttgtag ataaagtagc aggacgcttt ggtaagaaag
     6361 aagaaagttt agacaatctt aaattaccta agttcctctc aatcttccac gatacagttg
     6421 ttgcatctgc taccttgatg ctcgtattct tcggagccat tcttttaatc ttgggtccag
     6481 acattatgtc taataaagaa gtcatcactt caggaactct attcaatcct gctaaacaag
     6541 atttctttat gtacattatc caaacagcct ttaccttctc agtttacttg ttcgttttga
     6601 tgcaaggtgt ccgaatgttc gtatctgagt tgacaaacgc cttccaaggt atttcaaaca
     6661 aattgttgcc aggttcattc ccagcggttg acgttgcagc ttcttatgga tttggttctc
     6721 caaatgctgt cttgtcagga tttacctttg gtttgattgg tcaattgatt acaattgttt
     6781 tgctcatcgt ctttaaaaat ccgattctta ttattacagg atttgtacca gtgttctttg
     6841 acaatgcagc cattgcggtc tacgctgata aacgcggcgg atggaaagcg gctgttatcc
     6901 tttcctttat atcaggtgtc cttcaagttg ctctaggagc tctttgtgtg gcccttctcg
     6961 atttggcatc ttatggtggc taccatggaa atatcgactt tgaattccca tggcttggat
     7021 ttggatatat cttcaaatac cttggtattg ttggttatgt acttgtgtgt ctcttcttgc
     7081 ttgttattcc tcaacttcaa tttgccaaag caaaagataa agagaaatat tacaacggtg
     7141 aagttcaaga agaagcttag tatctagaaa aggagaaata aaatggttaa agtattagca
     7201 gcgtgcggaa atggaatggg ttcatcaatg gttatcaaga tgaaggttga aaatgctctc
     7261 cgtaagctta atcaaacaga ttttacagtc aattcatgca gtgtcggtga agctaaaggt
     7321 ttagcagtag gatatgacat tgtaatcgct tctcttcatt tgattcaaga attggaaggg
     7381 cgaactaatg ggaagttaat tgggcttgat aacttgatgg atgataaaga aatcaccgaa
     7441 aaactcagtc aagcactaca gtaaaaggtt ggagggggct ggacagaaac tgagagttat
     7501 cgtttctgtc cagccccctc tttaaataaa ggaggcagat atgaatttaa aacaagcttt
     7561 aattgacaat gactcgatcc gactaggttt agaggctaac aattggaaag aagcagtcaa
     7621 ggtagcagta gatcccttga ttgaaagtgg ggcaattttg ccagagtatt acgatgctat
     7681 cattgaatcg actgaagagt atgggcctta ctatatcttg atgccaggta tggctatgcc
     7741 ccacgctaga cctgaagctg gtgtgcaaag tgatgccttt tcattgatta ccttacaaaa
     7801 tcctgttgta ttttcagatg ggaaagaggt atctgttttg ttggcactag cagcaacaag
     7861 ttcaaaaatt cacacaagtg tagccattcc acaaattatt gccctgtttg aattagaaga
     7921 ttctattgca cgtttacagg cttgccagac taaagaagat gtcttggcta tgattgaaga
     7981 atctaaggat agcccttatc tcgaaggatt ggatttggaa agttagaaag aggaataaag
     8041 aaatgacaaa aagaatacct aatttacaag ttgcattaga ccattcagac ttgcaaggag
     8101 cgattaaagc agctgtttct gttggtcagg aagtagatat tatcgaagct ggaactgttt
     8161 gcttgcttca agttggaagt gaactggctg aagtcttgcg tagccttttc ccagataaga
     8221 ttattgtggc agacacaaaa tgtgctgatg ctggtggaac agttgctaaa aataatgcgg
     8281 ttcgtggagc agactggatg acttgtatct gttgtgcaac catccctact atggaagcag
     8341 ctctaaaggc tatcaagact gaacgaggag aacgaggcga aatccagatc gagctttatg
     8401 gcgattggac ttttgaacaa gctcagcttt ggctagatgc aggtatctca caagctattt
     8461 atcaccaatc tcgtgatgct cttcttgctg gtgaaacttg gggtgaaaaa gaccttaata
     8521 aggttaaaaa actcattgac atgggcttcc gtgtatctgt aacaggtggt ctagatgtag
     8581 atactctcaa actctttgaa ggtgttgatg tctttacctt tatcgcaggt cgtggaatta
     8641 cagaggctgc ggatccagca ggagcagcgc gtgccttcaa ggatgaaatc aaacgaattt
     8701 gggggtaaat catggtacgt ccaattggaa tttatgaaaa ggcaacccca acacacttta
     8761 cttggctaga acgtttaaat tttgccaagg agttaggctt tgattttgtc gagatgtcta
     8821 ttgacgaacg tgacgagcgt ttagcaagac ttgactggag taaggaagaa cgcttggaag
     8881 ttgtcaaaga aatctatgaa actggtgttc gtattccttc tatctgtttt tcaggccatc
     8941 gtcgctaccc attgggttca aaagatccag ttctagagga aaaatctcta gaactcatga
     9001 aaaaatgtat cgaattagct caagacttgg gagttcgtac gattcaatta gctggttacg
     9061 atgtttacta tgaggaaaag tcaccccaga cacgccaacg ttttatcaaa aatttgagaa
     9121 aagcctgtga ctgggctgaa gaagctcagg tggtacttgc tattgaaatt atggatgatc
     9181 ctttcatcaa tagcatcgaa aaatatttgg ctatagaaaa agagattgac tctcccttcc
     9241 tctttgtata tccagatatt ggtaatgtgt ctgcatggca taatgatatc tatagtgagt
     9301 tttatcttgg tcatcatgcc atcgcagctc tccatctcaa ggatacttat gcagtgacag
     9361 aaagttcaag gggccagttc cgagatgtac cttttgggca aggttgtgtc aaatgggaag
     9421 aagctttcga tattttaaag gaaaccaatt ataatggacc tttcctaatc gaaatgtggt
     9481 ctgaaaattg tgaaacagta gaagaaacac gcgcagccat tcaagaggcg caagcttttc
     9541 tctatccact cattaagaaa gcaggtttga tgtaagatga atcaagtaat caatgctatg
     9601 cgtaaacgag tctgtgatgc caatcaatca ttgccaaaac atggacttgt caaatttacc
     9661 tgggggaatg tatctgaagt caatcgcgaa ctcggtgtca ttgttatcaa accatcaggc
     9721 gtggattatg acgaattgac acctgaaaac atggtagtga ctgatctaga tggtaagatc
     9781 ctagaagggg atttaagacc atcttccgac ctcccaactc atgtgcaatt atataaggct
     9841 tggtcagaaa ttggtagtgt ggttcacacc cattcgacag aagctgttgc ttgggctcag
     9901 gcaggtcgtg atattccttt ctacggaaca acccatgcag attatttcta cggttcaatc
     9961 ccttgcgccc gtagtttgac caaggacgaa gtagaagtgg cctatgaaaa agatactggc
    10021 ctggttatcg tagaagagtt tgaacatcgc ggacttaacc cggttgaagt accaggaatt
    10081 gttgtacgca atcacggtcc attcacctgg ggcaaaaatc cagagaatgc tgtttatcac
    10141 tctgtcgtac tagaggaagt atcaaagatg aatcgcttta cagaacaaat caatccaaga
    10201 gttggacctg ctccccagta catactagaa aaacactacc aacgtaaaca tggaccaaat
    10261 gcttattatg gtcaaaagta agaacgatga aggaggagaa aaagataaat ttagctcctc
    10321 tttttacatt tgatttttat tgagagtaaa gttggagttg aagtaatttt aaaagatttt
    10381 ttagaaatag cgcttgatat atatatggta aaataaaaag aattgctgtg atatcaatag
    10441 atttggggga ttttttaata tggtactgga taaggcaagt tgtgatttgc ttcaatattt
    10501 gatggatcaa gaaacgtcca aaacgattat ggcgatttcg aaagatttga aagagtcaag
    10561 aaggaaaatt tattatcaca ttgacaaaat caatgctgct ctgggtgacg aggcgcttca
    10621 catcattagt attccacgaa ttggtattca cttaacggaa gagcagagag atgcttgttg
    10681 taaactatta tcggaagtag attcgtacga ttatatcatg agtgcgcatg aacgtatgat
    10741 gataatgtta ctatggatag gtatttctaa agaacgtatt acgattgaaa aattgataga
    10801 gttaacagag gtatctagga atactgttct caatgatttg aatagtattc gttatcaact
    10861 aactttggaa caatatcagg tgatcttgca agtgagcaag tcacagggat accaccttca
    10921 tgcccaccct cttaataaaa ttcagtatct tcaatcgctt ctatatcata tttttatgga
    10981 agaaaatgcc acttttgtat ctattttaga agataagatg aaagagaggt tagatgatga
    11041 gtgtttgctt tctgttgaaa tgaaccaatt ttttaaggaa caggttcctt tagttgaaca
    11101 agatttaggg aagaaaataa accatcatga aataactttt atgttgcagg ttctacctta
    11161 tttgctgtta agctgtcata atgttgaaca gtatcaagaa agacatcagg atatagagaa
    11221 agaattttct ttgataagaa aaagaataga gtatcaggtg tctaagaaat taggagaacg
    11281 gttgtttcaa aagtttgaaa tttctttgtc aggacttgaa gtttctcttg tagctgttct
    11341 cctcctctcc tatcgtaaag atttggatat tcatgcagaa agtgatgatt ttcggcaatt
    11401 aaaacttgct ttagaagaat ttatctggta ttttgaatca caaatccgaa tggagattga
    11461 gaacaaggat gatttgttac gaaatttgat gatccactgt aaagccttgt tatttagaaa
    11521 gacttacggt attttttcta aaaatcctct aacaaaacaa attcgatcca agtatggaga
    11581 attattttta gtcactagaa aatctgcgga aattttagaa ggagcatggt ttattcggct
    11641 aacagacgat gatattgcct atttgacgat tcatattgga ggatttttaa aatatacacc
    11701 atcatctcaa aaaaatatga aaaaagttta tctcgtttgt gatgaaggtg ttgcggtttc
    11761 gagacttttg ctgaaacaat gcaaacttta ttttccaaat gagcaaattg acactgtatt
    11821 tacaacagaa caatttaaga gtgtggaaga tattgcacaa gttgatgtag tgattactac
    11881 taatgatgat ttggatagca gatttccgat tttaagggtt aatcctatcc ttgaagcaga
    11941 agatattttg aaaatgctag actatcttaa acacaatata tttcgtaata agagcaaaag
    12001 tttcagtgaa aatctttcta gtcttatttc gtcttatatt gtagacagca agttggctag
    12061 taagttccaa gaagaggttc aaacacttat aaatcaagaa atagtagttc aagctttttt
    12121 ggaagatatt tgaaggacag tccaatgatg aacacaaacc tgtgtttttc ttggtctttt
    12181 ttagtgtttt gaagggtggt atactaatct caaagataac aattatatcc aaaggaggca
    12241 acatatgcca aacgtcaaag aaattacaag agagtcatgg attttagcca ctttcccaga
    12301 gtggggaaca tggttgaacg aagaaatcga agaagaagtc gtacctgaag gcaactttgc
    12361 catgtggtgg ctaggcaact gtggtacttg gattaagaca ccagctggtg ctaacgttgt
    12421 catggacctt tggtcaaacc gtggaaaatc aaccaaaaaa gtgaaagata tggttcgtgg
    12481 gcaccaaatg gcaaatatgg caggtgttcg taagctgcaa ccaaacttgc gtgttcagcc
    12541 aatggttatc gatccatttg ctatcaacga actagactat tacttagttt cacacttcca
    12601 cagtgatcat atcgacccat acacagctgc agcaattctc aataatccta agttagagca
    12661 tgttaagttt atcggtcctt accactgtgg acgaatctgg gaaggatggg gtgttccaaa
    12721 agaacgtatc atcgttgtta aaccaggtga cactatcgaa ttaaaagata tgaagattca
    12781 tgcagtagaa tcatttgacc gtacttgctt ggtaactctc ccagtgaacg gtgctgatga
    12841 gacaggcggt gaacttgctg gcttggctgt tacagatgaa gaaatggctc aaaaggctgt
    12901 taactatatc tttgaaacac caggtggaac catctatcat ggtgcagatt ctcacttctc
    12961 aaactatttt gcaaaacatg gtaaagactt taaaattgat gttgctttga ataactatgg
    13021 tgaaaatccg gtaggtatcc aagacaaaat gacatctatc gaccttcttc gtatggcaga
    13081 aaatctgcgt accaaagtca ttatcccagt tcactatgat atctggtcta acttcatggc
    13141 ttctactaat gagattctag aactttggaa aatgcgaaaa gatcgcttgc aatacgattt
    13201 ccatccattt atctgggaag ttggcggtaa gtacacttat cctcaagatc aacacttagt
    13261 agaataccat catccacgtg gttttgatga ttgttttgaa caagactcta acattcaatt
    13321 taaagctttg ctataatata aaaatattct ttggaggtta tccgtatgtt gcatgatacg
    13381 gataattttc atataatagt aaaatagaat gtgtgattca ataatcacct caaatagaaa
    13441 ggaaattcta tgtcaaatct atctgttaat gcaattcgtt ttctaggtat tgacgccatt
    13501 aataaagcca actcaggtca tccaggtgtg gttatgggag cggctccgat ggcttacagc
    13561 ctctttacaa aacaacttca tatcaatcca gctcaaccaa actggattaa ccgcgaccgc
    13621 tttattcttt cagcaggtca tggttcaatg ctcctttatg ctcttcttca cctttctggt
    13681 tttgaagatg tcagcatgga tgagattaag agtttccgtc aatggggttc aaaaacacca
    13741 ggtcacccag aatttggtca tacggcaggg attgatgcta cgacaggtcc tctagggcaa
    13801 gggatttcaa ctgctactgg ttttgcccaa gcagaacgtt tcttggcagc caaatataac
    13861 cgtgaaggtt acaatatctt tgaccactat acttacgtta tctgtggaga cggagacttg
    13921 atggaaggtg tctcaagcga ggcagcttca tacgcaggct tgcaaaaact tgataagttg
    13981 gttgttcttt atgattcaaa tgatatcaac ttggatggtg agacaaagga ttcctttaca
    14041 gaaagtgttc gtgaccgtta caatgcctac ggttggcata ctgccttggt tgaaaatgga
    14101 acagacttgg aagccatcca tgctgctatc gaaacagcaa aagcttcagg caagccatct
    14161 ttgattgaag tgaagacggt tattggatac ggttctccaa acaaacaagg aactaatgct
    14221 gtacacggcg cccctcttgg agcagatgaa actgcatcaa ctcgtcaagc cctcggttgg
    14281 gactacgaac catttgaaat tccagaacaa gtatatgctg atttcaaaga acatgttgca
    14341 gaccgtggcg catcagctta tcaagcttgg actaaattag ttacagatta taaagaagct
    14401 catccagaac tggctgcaga agtagaagcc atcatcgacg gacgtgatcc agtcgaagtg
    14461 actccagcag acttcccagc tttagaaaat ggtttttctc aagcaactcg taattcaagt
    14521 caagatgcct tgaatgttgt ggcagctaaa ttaccaacct ttctaggtgg atcagctgac
    14581 cttgctcact caaacatgac ttatatcaag acggatggac ttcaagacga tgccaatcgc
    14641 ttgaatcgta acattcagtt tggtgttcgt gaatttgcaa tgggaacaat cttgaacggg
    14701 atggcccttc atggtggact tcgtgtatac ggtggaactt tcttcgtctt ctctgactat
    14761 gtgaaggcag ctgtccgctt gtcagcctta caaggacttc ctgtgactta tgtctttacc
    14821 catgattcaa tcgcagttgg ggaagatggt ccgactcatg aaccagttga gcatttagca
    14881 ggtcttcgtg ctatgccaaa tctaaatgtt ttccgtccag cagatgcgcg tgaaacgcaa
    14941 gcagcttggt accttgcagt gacaagtgag aaaacaccaa ctgcccttgt cttgacacgt
    15001 caaaatttga ctgttgaaga tggaacagac ttcgacaagg ttgctaaagg tgcttatgtt
    15061 gtatatgaaa atgcagccga ctttgatacc atcttgattg cgacaggttc agaggttaat
    15121 cttgctgtct cagctgctaa agaattggct agtcaaggcg aaaaaatccg cgtagtcagc
    15181 atgccatcta cagatgtctt tgataaacaa gatgcagctt acaaggaaga aattcttcca
    15241 aatgcagtcc gccgtcgtgt tgcagtcgaa atgggtgcaa gtcaaaactg gtacaaatat
    15301 gttggtctcg atggtgccgt tctaggtatt gataccttcg gagcctctgc cccagcacca
    15361 aaagtattgg cagaatatgg ctttactgta gaaaatcttg taaaagttgt tcgaaacttg
    15421 aaataatcct aaaaatcagg gcgtaagctc tggtttttct taccagaaaa gtaaggtaca
    15481 atcttgtaaa agtagctgaa atttgatata gtagtcctat gtaaaagaca aaggagaaaa
    15541 caatgaatcc aaatattact tttttaatca tgcttgtagg tatgatggcc ttgatgttct
    15601 ttatgcaacg ttctcaaaag aaacaagctc aaaagcgtat ggaaagctta aataaactac
    15661 aaaaaggcta tgaagtgatt acaatcggtg gactttatgg aacagtcgat gaagtagata
    15721 cggagaaggg aacaatagtt cttgacgtag agggagttta cttgactttt gaactagctg
    15781 ctatcaagac agtattaccg ctgaaagaaa cagcttcact agaaggcgca attgaaaaat
    15841 aagacgggat cacgaactcc cgtttttcta taaaagaaag gaaatgggat gaaaaaatta
    15901 gtctttgtct gtctgggaaa tatttgccgt agccctatgg ccgagtttgt tatgaaatca
    15961 atgacagata actacgaaat ccaaagtcga gcaacttcct cttgggaaca tggcaatccg
    16021 attcataagg ggactcaggg aatttttcaa gagtatgaga ttccttatga caagaacaag
    16081 acatcgcttc agattagtaa ggaagatttt gaagcctttg attatattat cggaatggac
    16141 gcttcaaata tttctgactt acgtcagatg tgtccagtag actgtcaaga taagatttac
    16201 tcattttcat ctgaaagtgt tccagaccct tggtatacag gagattttga agaaacctat
    16261 cgacgtgttc aagagggctg tcaagcttgg ttagaacgtt tagaaaagga gagttaaggt
    16321 ggaaaatctt acgaattttt acgaaaagta tcgtgtctat ctgactcgtc cacgtttaga
    16381 gcttttggca gtagttacca ttgttttctg tgctgtactc gtcttttttc taaatattcc
    16441 aggaaaaggt gtcttaaaac tcgataatgg aacgattgtt tatgatggca gtcttgtccg
    16501 cggtaaaatg aatggccaag gtaccattac cttccaaaat ggagaccaat atacaggtgg
    16561 cttcaacaat ggagccttca acggaaaagg tacctttcaa tctaaagaag gctggaccta
    16621 cgaaggtgat tttgtaaatg gtcaggctga aggaaaaggg aaactaacaa cagaacaaga
    16681 agtcgtttat gaaggaactt ttaaacaagg cgtttttcaa caaaaataaa gcctccttat
    16741 caaaggaggt attattagaa tcgcaaggta agcgcttacc tgcaaatccc tttctttcca
    16801 aatccctctt ccaagcaagt ttgtgaaata aaaaatattt gaaataaatt tcacaaactt
    16861 caaagataaa acctgataag aaaagaaaat gagaaaagtt tcacaagagt ttaaaaattc
    16921 tttgtgaaat gtttatgaaa ctgtttacaa agttgaaata atgcgttata ataaacttgt
    16981 gaaaaaatta acaaaggata tcgttccttg aaagctatgg aggaaaatat ggctgataaa
    17041 aaaactgtga caccagagga aaagaaactc gttgctgaaa aacacgtaga tgagttggtt
    17101 caaaaagctc tagttgccct tgaagaaatg cgtaaattgg atcaagaaca agttgactac
    17161 atcgttgcca aagcatcagt agcagctttg gatgcccacg gagaattggc tttacatgcc
    17221 tttgaagaaa caggacgtgg tgtatttgaa gataaagcaa ctaagaactt gtttgcttgt
    17281 gaacacgtgg taaacaacat gcgtcacact aaaacagttg gcgttatcga agaagatgat
    17341 gtaacaggat tgaccctgat tgctgaacca gttggtgttg tttgtggtat cactccaaca
    17401 acaaacccaa catcaacagc gatcttcaaa tcattgattt cattgaagac acgtaatcca
    17461 atcgtctttg ccttccatcc atcagctcaa gaatcatcag ctcatgcagc tcgtatcgtc
    17521 cgcgatgcag ctatcgcagc tggtgctcct gaaaactgtg tacagtggat cactgaacca
    17581 tctatggaag caactagtgc ccttatgaac cacgaaggta ttgcaacaat ccttgcaaca
    17641 ggtggtaatg ccatggttaa agccgcatac tcatgtggta aaccagctct tggggtaggt
    17701 gccggaaacg ttccagctta tgttgaaaaa tcagcaaaca ttcgtcaagc agcacacgat
    17761 atcgtcatgt ctaaatcatt tgataacggt atggtctgtg catctgaaca agcagttatc
    17821 attgataaag aaatttacga tgaatttgta gcagagttca aatcttacca cacttacttt
    17881 gtaaacaaaa aagaaaaagc tcttcttgaa gagttctgct tcggcgtcaa agcaaacagc
    17941 aaaaactgtg ctggtgcaaa attgaacgct gacatcgttg gtaaaccagc aacttggatt
    18001 gcagaacaag caggatttac agttccagaa ggaacaaaca ttcttgctgc agaatgtaaa
    18061 gaagttggcg aaaatgagcc attgactcgt gaaaaattgt caccagttat tgcagttttg
    18121 aaatctgaaa gccgtgaaga tggtattact aaggctcgtc aaatggttga atttaacggt
    18181 cttggacact cagcagctat ccacacagct gacgaagaat tgactaaaga atttggtaaa
    18241 gctgttaaag ctattcgtgt tatctgtaac tcaccttcta cttttggtgg tatcggggac
    18301 gtttacaatg ccttcttgcc atcattgaca cttggatgtg gttcttacgg acgcaactca
    18361 gttggggata acgttagtgc cattaacctc ttgaatatca aaaaagtcgg aagacggaga
    18421 aataacatgc aatggatgaa acttccttca aaaacatact ttgaacgtga ttcaattcaa
    18481 taccttcaaa aatgtcgtga cgttgaacgt gtcatgatcg ttactgacca tgccatggta
    18541 gagcttggtt tccttgatcg tatcatcgaa caactggacc ttcgtcgcaa taaggttgtt
    18601 taccaaatct ttgcggatgt agaaccggat ccagatatca caactgtaaa ccgtggtact
    18661 gagattatgc gtgccttcaa accagatacc atcatcgcac tcggtggtgg gtctccaatg
    18721 gatgctgcca aagtaatgtg gctcttctac gagcaaccag aagtggactt ccgtgacctt
    18781 gtccaaaaat tcatggatat ccgtaaacgt gccttcaagt tcccattgct tggtaagaag
    18841 actaaattca tcgcgattcc aactacatct ggtacaggat ctgaagtaac accatttgcc
    18901 gttatctctg ataaagcaaa caaccgtaaa tacccaatcg ctgactactc attgacacca
    18961 actgtggcaa tcgtagatcc tgctttggta ttgacagttc caggatttgt tgctgctgat
    19021 actggtatgg acgtattgac tcacgcgaca gaagcatacg tatcacaaat ggctagtgac
    19081 tacactgatg gtttagcact tcaagccatt aaattggtct ttgaaaatct cgaaagctca
    19141 gttaagaatg cagacttcca ctcacgtgag aaaatgcata acgcttcaac aatcgctggt
    19201 atggcctttg ccaatgcctt cctaggtatt tctcactcaa tggcccataa gattggtgcg
    19261 caattccaca caatccacgg tcgtacaaat gctatcttgc ttccatacgt tatccgttac
    19321 aacggtacac gtccagctaa gacagcaaca tggcctaagt acaactacta ccgtgcagat
    19381 gaaaaatacc aagatatcgc acgcatgctt ggacttccag cttctactcc agaagaaggg
    19441 gttgaatctt acgcaaaagc tgtctacgaa ctcggtgaac gtattgggat ccaaatgaat
    19501 tttagagacc aaggaattga cgaaaaagaa tggaaagaac attctcgtga attagccttc
    19561 ctggcttatg aagaccaatg ttcaccagct aacccacgtc ttccaatggt agaccatatg
    19621 caagaaatca tcgaagatgc atactatggc tacaaagaaa gaccaggacg ccgtaaataa
    19681 ttgtttatca gtctagaagc aagacaaaaa ctcaatttga gggaaagatc cagtaatttt
    19741 tctatgataa aaggcatcct atcaaggttt ttgaacacct gataggatgc ctttttatga
    19801 tattgaggcc tttttgccct ttttgaaaaa ctagaataga aacaaaatat ataatagatt
    19861 gaaactggaa tagtacactt ctgcttctaa aacattgtta gaattcgatt tgactgtcct
    19921 gatcgatttg tcctattcct atttcatttt actatataaa aaatat
//
LOCUS       DAVBHS010000022        19892 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVBHS010000022 DAVBHS010000000
VERSION     DAVBHS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19892
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.022"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(177..1427)
                     /gene="ilvA"
                     /locus_tag="VQ651_000555"
     CDS             complement(177..1427)
                     /gene="ilvA"
                     /locus_tag="VQ651_000555"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HET2753585.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(1477..1833)
                     /locus_tag="VQ651_000556"
     CDS             complement(1477..1833)
                     /locus_tag="VQ651_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET2753586.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(2071..2307)
                     /locus_tag="VQ651_000557"
     CDS             complement(2071..2307)
                     /locus_tag="VQ651_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753587.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(2414..3436)
                     /gene="ilvC"
                     /locus_tag="VQ651_000558"
     CDS             complement(2414..3436)
                     /gene="ilvC"
                     /locus_tag="VQ651_000558"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET2753588.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVVEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(3502..3978)
                     /gene="ilvN"
                     /locus_tag="VQ651_000559"
     CDS             complement(3502..3978)
                     /gene="ilvN"
                     /locus_tag="VQ651_000559"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET2753589.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDKVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(3971..5671)
                     /locus_tag="VQ651_000560"
     CDS             complement(3971..5671)
                     /locus_tag="VQ651_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET2753590.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(5872..7539)
                     /locus_tag="VQ651_000561"
     CDS             complement(5872..7539)
                     /locus_tag="VQ651_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET2753591.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(7542..7907)
                     /locus_tag="VQ651_000562"
     CDS             complement(7542..7907)
                     /locus_tag="VQ651_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2753592.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(8064..8252)
                     /locus_tag="VQ651_000563"
     CDS             complement(8064..8252)
                     /locus_tag="VQ651_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET2753593.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(8368..8544)
                     /locus_tag="VQ651_000564"
     CDS             complement(8368..8544)
                     /locus_tag="VQ651_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET2753594.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            complement(8655..8804)
                     /locus_tag="VQ651_000565"
     CDS             complement(8655..8804)
                     /locus_tag="VQ651_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET2753595.1"
                     /translation="MDKVETTIKSLCVHHYNLKFYVFNDDLPREWFQLMEKRLETLNS
                     EIVNV"
     gene            complement(9116..10660)
                     /locus_tag="VQ651_000566"
     CDS             complement(9116..10660)
                     /locus_tag="VQ651_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET2753596.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            10873..11175
                     /gene="gatC"
                     /locus_tag="VQ651_000567"
     CDS             10873..11175
                     /gene="gatC"
                     /locus_tag="VQ651_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET2753597.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            11175..12641
                     /gene="gatA"
                     /locus_tag="VQ651_000568"
     CDS             11175..12641
                     /gene="gatA"
                     /locus_tag="VQ651_000568"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET2753598.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDANNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDTKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            12641..14083
                     /gene="gatB"
                     /locus_tag="VQ651_000569"
     CDS             12641..14083
                     /gene="gatB"
                     /locus_tag="VQ651_000569"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET2753599.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDLHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            14212..14772
                     /gene="efp"
                     /locus_tag="VQ651_000570"
     CDS             14212..14772
                     /gene="efp"
                     /locus_tag="VQ651_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET2753600.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            14794..15183
                     /locus_tag="VQ651_000571"
     CDS             14794..15183
                     /locus_tag="VQ651_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2753601.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGVYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            15176..15598
                     /gene="nusB"
                     /locus_tag="VQ651_000572"
     CDS             15176..15598
                     /gene="nusB"
                     /locus_tag="VQ651_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET2753602.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTNVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKVGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(15647..16024)
                     /locus_tag="VQ651_000573"
     CDS             complement(15647..16024)
                     /locus_tag="VQ651_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2753603.1"
                     /translation="MVLSNGQPTFLSTILSFVLLVIIGPVFEETLDRGYFMNTFFPKS
                     KYYLDVILSGLIFGISRLILSHRDPISLLYYSLIGFFFALVYRSTDNLRLTILCHSFF
                     NFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(16365..16550)
                     /gene="briC"
                     /locus_tag="VQ651_000574"
     CDS             complement(16365..16550)
                     /gene="briC"
                     /locus_tag="VQ651_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HET2753604.1"
                     /translation="MTGTETFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            complement(16709..17476)
                     /locus_tag="VQ651_000575"
     CDS             complement(16709..17476)
                     /locus_tag="VQ651_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HET2753605.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(17473..18339)
                     /locus_tag="VQ651_000576"
     CDS             complement(17473..18339)
                     /locus_tag="VQ651_000576"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HET2753606.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(18376..19743)
                     /gene="accC"
                     /locus_tag="VQ651_000577"
     CDS             complement(18376..19743)
                     /gene="accC"
                     /locus_tag="VQ651_000577"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET2753607.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(19755..>19892)
                     /locus_tag="VQ651_000578"
     CDS             complement(19755..>19892)
                     /locus_tag="VQ651_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabZ"
                     /protein_id="HET2753608.1"
                     /translation="VVPGDQLVMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIG
                     N"
BASE COUNT         5800 a         4610 c         3630 g         5852 t
ORIGIN      
        1 aggaaatcaa aatgaaggat ggaaatctga gccaaacatc ataagtgcta taatgaacag
       61 aagtaagaat tgcacgataa gttttctaaa accataggaa tcgtcccctt tcttcatctt
      121 cattatagca catatacatc agttgtacaa ataatatgat atttttttat tagtcctcag
      181 acaagcatat tataaagcgt ttcattacca tttaagttaa tataagctgg atcaaaacct
      241 tccattctac ggatcaaacc tgcataatca tgcttatctg ccaaagcgat cccaatcaat
      301 actgggcctg ttcccttgct agctcgtttg atatactcaa aacgtgtgat atcatcattt
      361 ggccccagga tatcatttac aaactcacgc aaagctcctg gacgctgtgg gaaattgacc
      421 acaaagtaat gtttgatacc atcataaatc aaggcacgct cttccatttc tggcatacgg
      481 ttgatatcat tatttcctcc agaaatgata caacaaatgg ttttcccctt aatatattca
      541 gctaaaacct ctaaagaggc gatactagcc gctccagcag gttctgcgac tatcccttgc
      601 ttagagtaaa ggtcaatcaa ggtttcagaa atcaatccct catcgacacc taccaaagtt
      661 tgaatatgtt gacgagttgc ttcataggtc aactgaccta ccttttgcac agcaatccca
      721 tccgcaaatt tatcaatttc cttgagtttg actggacctc cagcctcaaa ggcagctttc
      781 atggaacgcg ctccattcgc ctctactccg ataacctcaa tctctggact tgtttccttg
      841 atataggtag aaaccccggc aatgagaccg ccaccaccaa caggaaccaa gacagcatca
      901 aaatcaatcg attcttttcg agcttcttct aaaatctcat aagcaactgt tccttgacct
      961 gcttgaacat gagcatcatc aaaaggatca ataaaggtac gattttcaga gactgtaaat
     1021 tcttgagctg ctttggctga ggcatcaaag gtatctccaa ctagtttaat agttacaaaa
     1081 tccccaccaa aaaagcgaac ctgaccaatc ttttgttgtg gcgtagtaat aggcataaag
     1141 atagtagcag gaattttcat ttcattacaa gtataggcta ctccctgcgc atgatttccc
     1201 gcagaagcgc agactacccc acgttcacgt tcttccttgc tgagctggga aatggcataa
     1261 taggcaccac gaattttaaa ggagcgaaca cgctgggcat tttctttttt caaataaatc
     1321 ttagcaccat acttctccga taaataatga tcgtaatcca gtggagtatt cacaaccaca
     1381 ccgttcaaga ccttgtgagc cttgattata tcttttgaac ttaacatata aaacctcttt
     1441 ttctataaaa aacagtcact aatgactgtt ttttagttat tcatctccga aaacttcagt
     1501 aacactttca tggtatttat gagccatacg aatcgaaaac tctttaggaa gttcataacg
     1561 atttgtggaa atccaatcat ataaccattc taccaaactt tcttcactat cattacgatg
     1621 gaagataaaa tagtttaaaa tttctttata ttctacatca ttcaaatcca acgtgtctac
     1681 aaattgacta cgaggaacaa aattagaatc taattttctt agttgatctg tcaattctcc
     1741 caattgtaaa tccaactgaa aaaatagttt ttgcagttgc ctatcagttt catcacatcc
     1801 tgtcttgata gaaaacaaat tggtaatatt catatgattt caacccctaa ataagtacat
     1861 ctaatatccc acgaatagct gaagctaaag ttttcgcagt ccagtcattt aaacccgatt
     1921 ctttcaatcc agatgcaata atttgctcta tttgatataa accgccatct atcgtctcat
     1981 ttattctgcg aagaggacct tcaatatagg ataaagcttc tccgaatcta acagctgatt
     2041 taggtcctaa tgatttacca actatatttg ttaatgtttt ttatttctca aaatccattt
     2101 cgctatataa cgagcagatt tagtcgcacc agctctctca aacggagaat gaatttcaag
     2161 accttcttct ctagctattt gtgccaattt gaagaattgt tcaaaggtca tatgcaacct
     2221 accatcttca aaattagcat catcataaat gttaattaca gcattttcat catacgacca
     2281 atctgcttta ttttggtctg ctagcactat taccgacggt gtaattacat tatacaacta
     2341 gaaccaataa taacgatgca atttttctca taatcttttt cataaaatca tctccagcgc
     2401 tatatatttc taattagtta tagattttga atgcatcatc gtcgttttta ccaacaaatg
     2461 gcattgcttt acgcaattct gcaccaactt tttcaatttc aaggttagct gcttgttcac
     2521 ggtaagcagt caattttgga cgtccagctt tatagtcatt tacaaagtca tttgcaaatt
     2581 taccattttg gatgtctgcc aagacagcct tcatattttc tttaacttgt tcagtgatta
     2641 cacgtggacc tgatacatag tcaccgtatt cagcagtgtt tgaaatagat tgacgcattt
     2701 tcttgaatcc accttcgtag atcaagtcaa cgatcaattt catttcgtga agaacttcaa
     2761 agtaagccaa ttctggagcg taacctgctt ctgtcaagac ttcgaaacct gcttcgataa
     2821 gggcagtcaa accaccacaa agtacagctt gttcaccaaa caaatcttct tcagtttctt
     2881 ctttgtaagt tgtttcaaga agacctacac gagccgctcc aacaccttta caccagtcca
     2941 tagcaatgtt tttagcattt cctgttgcat cttggtatac tgcataaaga gctggaacac
     3001 caaatccttc ttcgtaagta cgacgtacca agtgtcccgg tcctttagga gcacacatga
     3061 agacatctac atccgcagga actttgataa attcaaagtg gatgttgaaa ccatgggcaa
     3121 atccaactgc gtttccagct tccaagtttg gagcgatttc tgcttcgtac aattcttgtt
     3181 gaatttcgtc tggcgccaag atcatgataa catcagccaa cttagtagct tctactactg
     3241 tgtaagtatc aaatccatct tcttttgctt tatcaaaaga tttacctgga cgtacaccga
     3301 taataacgtc acgacctgaa tcacgcaagt tttgagcatg cgcatgtcct tgtgaaccat
     3361 aaccgataac ggcgattttt ttaccgtcaa gtgctgctac tttaacatct ttttcatatt
     3421 ccatttgaac tgtcatagct tttctctctt ttctattatt tattgccttt taggctggtt
     3481 taataaattt aagttggatt tttaatcgcg ggtaaatcca gttgcacccg ttcgagcaat
     3541 attgcgaata ccgtatgggc gaatgactcg caatagggct tcgctctttt ctgcatttcc
     3601 cgtcatctga atggtaatcg agcttggcgc tacgtctacc actgttgcac ggaaaggttg
     3661 aataatcgct aaaatctcag ctctcttctc agctggcgct gacatcttaa ccaaaatcac
     3721 ctcgcgctcc aaatgaggct tgtctgtaat atctcgaatg cgaatcacat caatctgacg
     3781 attgagctgt ttgatgattt gctccacttt atcatgagaa gcaacatcaa taataatagt
     3841 gatacgcgat acattcggat cttctgttgc tccaacagag atgctttcaa tattaacctg
     3901 acgacgagat aggacacctg taaagcgatt gaggactcct gaacgatttt gtagttttgc
     3961 tgttaacatt ctacgcatgg aactgcaccc ccaacatctc atgattactc ttaccagccg
     4021 gtaccattgg taacacctgt tccttacgag aaatatctac ctcaattagc ataggaacat
     4081 cctcagtgat gacttcaagg tcttgagcca aggtctcagg attgtcaaac ttatagtttt
     4141 taataccata agcctgcgcc atcaattgga aatcaggaag ggtatcaaag accgactctg
     4201 atgttctgcc ttcatagaag gattcctgcc actggcgaac cattccaagt gaatgattgt
     4261 tcagcataac caccttgatt ggcaccttgt aaatattcaa aatagccaac tcctggttgg
     4321 tcatttggaa accaccatcc ccaacaaaca agactacttc cttatctggg ttagcaattt
     4381 tagcaccgat tgctgctgga attccaaagc ccattgttcc caaaccacct gaagtcacta
     4441 actgacgctc attttggtag ggataatact gagctgtcca catttggtgt tgaccaacgt
     4501 ctgttaccac aatggcatct ccattcgtca attcaccaat tcgttcaata actgcttgcg
     4561 gttgaaccac acgctctttc ttgtcataag aacgaacacg attcttgtct ttagtgactt
     4621 tctcaatcca cttttcagta ttgttgtgaa ctgttggttc tgctagcaac atttgcaagg
     4681 ccttcttagc atctccaact acaggaatgt ctgcactgat aatcttgcca atctcagctg
     4741 ggtcaatatc aatgtgggca accttagcat tcttagcgaa agtcttagga ttccccgtca
     4801 aacggtcatc gaaacgagaa ccaatactaa tcataaagtc cgcttccgtc atggcaatat
     4861 ttgctgcgaa tgacccgtgc atgcctccca ttccaagaaa gagtgggtga ctcgttgcaa
     4921 tcgttccttg tcccaaaaga ctggttacca ctggaatttg atagcgttct gcaaattcat
     4981 ttagttccgt agcagcctca gcataactaa ttccaccacc agctaacaag actggctttt
     5041 tagccttgga caattgcttc aagattttct tgatttgcat atcattcggc tcaagagtcg
     5101 gctgataact tggtaaattc acttctggtg aataaatgaa gtctgtttct aaagcagata
     5161 tgtcttttgg taggtcaatt acaactggcc ctggacggcc tgtagttgcg atatggacag
     5221 cttccgtaat gatacgcgga atatcagctg tctcacgaac ttggtaattg tacttagtga
     5281 ttggcatggt aattcctacg atgtctgcct cctgaaaggc atccttccca atccctgctc
     5341 gcgccacctg acctgtaaag accaaaaggg gaacgctatc gctcatagca tccgcaatcc
     5401 ctgtaatggc atttgttgct cctggtccac tagtgacgac ggcaacaccc aactttccag
     5461 ttgatttggc ataaccttca gcttcatgca aacaaccttg ctcatggcgc cctagaatgt
     5521 ggcgaatgcc tttaaaatta tatatcgcat cataaaaagg caaaaccgca ccaccaggat
     5581 aaccaaagat ggtatcaact cctaaatcac gaagtgtttc caaaactagg tccgaccccg
     5641 tcttaggaga ttctaaactg attttctcca ttgttcccct ttctttcctc ttaaaaataa
     5701 cttgttacta tcataccatt ttttcaaaat ttttcaagat aaaagaagaa attttctgaa
     5761 ttttctattt taacgtttat ttatgaatgt tatttttgtt tttattaaaa agataccgat
     5821 ttcatttact aaaaaagaaa aaagaactga tttctcagtc cttcattaat cttattccac
     5881 actaaatagg tatgggtaaa caggttgttg accttggtga atctcgactt caacgtcttc
     5941 gaattcttct acgatttctt gagcgatttc attggcaagt tcttcgcttc cgtcttcacc
     6001 gacatagaag gttacgattt cactatcttc atccaacata tgtttcaagg tttcagtcaa
     6061 tgtttggtgc atatcagggt ttgacacaag aatttttcca tccaccatac ctagattatc
     6121 gttttcatgg atttctaagc catcgatcgt tgtatcacgc acggctgttg tgacgcttcc
     6181 gctaacgaca tcgctaagag cagctgtcat acgctcttgg ttttcttcaa tggacttgct
     6241 tggatcaaag gcaagaagac ttgtcatacc ttgaggaaga gtgcgagcct ctaccactac
     6301 tgctggttgc tccaaaactt ctgccgcaga ttgagctgcc atgaagatgt tcttgttgtt
     6361 tggcaagaag atgatgttac gggcattgac ctgttcaaca gccttgataa agtcttctgt
     6421 tgaagggttc atggtttgac cgccttcgat aacataatcc acgccttgag aacagaagat
     6481 atctgctaga cctttaccag ccaccacagc aatcaaagca tactcttttt cttcagccga
     6541 cttgataact tgagtagctt ctttctcaac ctgtgcttcg tgttggttac gcatattgtc
     6601 aacttttacc ttgaccaagc taccatattt gagaccttct tgcataacaa gtcctggatc
     6661 ttctgtatga acatggactt tgacaatttc atcatcgtta acaacgagga gagaatcccc
     6721 aagctcatcc aagtagttac ggaattcatc gtagtcaaaa tctttagcat aggttggacc
     6781 ttgcttaaga gctaccatga tttcagtaca gtaaccaaac gtgatgtcct cagtcgctac
     6841 gtgaccagct acagacttat gatgctctac attgatcatc tcactcatgt tggcaggagt
     6901 cgctacaaag tcctcagatg caatatattc gccagtaagg gctgaaagga aaccttcgta
     6961 gatgaagacc aatccttgac cacctgagtc cacaacgcca acttctttca atactggaag
     7021 catgtctggt gttttagcta gagctgtttt agcaccttcc aaggctgcgc gcatgacttc
     7081 aacagtgtca tctgtttgct cagctttttt cttagcaccg atagcagctc cacgagaaac
     7141 tgtcaaaatc gttccttcaa caggtttcat cactgcctta taggcaactt ccacacctga
     7201 ttggaaggcc agagccaagt cttgacctgt taactcgtct ttatccttga tagcttggga
     7261 aaatccacgg aaaagctgag acgtaatcac tcctgagttc ccacgcgcac ccatcaaaag
     7321 ccctttggca agaatgctcg ctacttctcc aactgtagaa gctggcttgt ctgcaacttc
     7381 tttagcacca ttttcaatgg tcattcccat atttgtccca gtatctccat ctggaactgg
     7441 aaagacgttt aatgaattga catattcagc ttgcttattc aagcgagttg atgcagcctg
     7501 caccatttct tgaaataagc tagtagtaat ttttgacacg gttattctcc tacaactttg
     7561 atattttgaa tgtagacatt tacagtctga gcagtaattc caagctggtt ttccaagcta
     7621 aaggcaacac gctcttgaat gttttttgac acttcactaa tctttgttcc gtagcttaac
     7681 acggtatata catcaactgc aatactgcca tcttcggctg cctttacgac gacaccttta
     7741 gaataatttt ccttacctag cagggcttgg aaattatctt tgagggcatt tttactagcc
     7801 ataccgacca caccaaaaat ctcagttgct gctccaccta cgacggttgc aatcacttca
     7861 tctgttagtt cgatttgacc atcttttgta ttaattttta cagtcatcct ttttacctca
     7921 actagttgat actctatttt atcatatttc agcccaagtg taaaagcaga atgatgtatc
     7981 agcggatatt tactctattt ttcaaacgat gttataccca caataaaaga aaaaagaccc
     8041 taaggtctcc tttgctttta ttattaaacg cgttcaactt tacctgattt caaagcacga
     8101 gctgaagccc aaactttttt aggtttacca tcgataagaa cagtaacttt ttgaaggttt
     8161 ggttttacgg cacgttttgt ttggttcatc gcgtgtgaac ggttgtttcc tgatacagtc
     8221 ttacgacctg taaagtaaca tactttagcc attgtgtttt cctcctatta gatctaatat
     8281 agcggatgtg ctagcaccac ataccgtact atgttatcac actttcttgt tttttgcaag
     8341 ggaattggaa gattttttat ttgtgtctta aatcaggtct tgcgtgacat tttctgctct
     8401 ccacatgcca tcgttgatta acagaacacc agaattaaaa ttatgtgtat aaaaatcatc
     8461 tctaactgca gctaagggta tagccgtcaa gtccaaatcc cacagctcat ctatcgattt
     8521 tcttacaaca atatctgaat ccaaatacag tacacgagac tcgcttacat actttggaat
     8581 aaaataccta aaaaagccgc atatgaaagt ccctcaaagg ggagacgata acctttcaga
     8641 atattactgt caatctaaac attcacaatc tcactattca aagtctctag tcttttttcc
     8701 atcaattgga accattctcg cggaaggtca tcattaaaaa cataaaactt aagattataa
     8761 tgatgaacac aaagagattt tattgttgtt tcaactttat ccatataagc attatctgca
     8821 cctaagacaa tcgctttttt ctcttttttc tcttctttca ctttttatct catttctttt
     8881 tattcccatc atatgtttct acgtaaccat tattttcgcc tattcgttcg taaaaccata
     8941 ccagtggaga ttttagatga agtcccatta cggtttacaa tttttacatt acgacacgga
     9001 gttttacaaa tcgatttcat ttgccaaacg tagttagtga ggcagttagc tagttcgcca
     9061 aatagcgact agcgtccaac aatttggaac tttagttcca attgttggta ctgagtcaca
     9121 tcttctcctc taactctacg tctggatact tgtcagcaaa ccagcggagg gcaaagtcat
     9181 tttcaaagag aaagactggt tggtcaaaac ggtctttggc taagatattg cgacttgacg
     9241 acatccgttc atccaagtcc tcaggcttga tccaacgaac ggtcttttta cccattgggt
     9301 tcataactac ttctgcattg tactcgcctt ccatgcggtg tttaaagact tcaaactgga
     9361 gttgacctac agcgcctagc atgtactcac ctgtttggta attcttataa agctgaacgg
     9421 ctccttcttg caccaattgc tcaatcccct tgtggaagga tttttgcttc ataacattct
     9481 tagcagaaac tttcatgaaa atttcaggag taaaggttgg cagtggttca aattcaaact
     9541 tgtttttacc aactgtcaag gtatccccaa cttgataagt accggtatcg taaaccccga
     9601 taatatcacc tgccacggca ttgatcacat tctcacgact ttccgccata aactgggtaa
     9661 cattagatag tttggccccc ttaccagtac gagggagatt gacactcatg ccacgctcaa
     9721 attcgcctga tacgatacgg acaaaggcaa tacggtcacg gtgacgagga tccatgttgg
     9781 cttggatttt aaagacaaag cctgagaaat ccttgtcata aggatccaca atttcaccgt
     9841 ctgttttctt gtgaccatgt ggttctggag caaacttgag gaagatttca aggaaggtct
     9901 gcacaccaaa gtttgtcagg gctgaaccga aaaagacagg cgtcaattct ccagccagaa
     9961 tagcttcctc tgaaaactca ttcccagctt catttaaaag ctcaatgtca tccttgactt
    10021 gctcgtagaa aggattgcta ccaaagagtt tgtccccgtc ttctagactg gcaaaacgct
    10081 catccccttt gtaaagctct aaacgttggt tatagaggtc atacaagccc tcaaaggctt
    10141 tccccatccc gataggccag ttcatagggt agctagcaat gcccaagatt tcttccaatt
    10201 cttgcaagag atccaaaggc tcacgaccgt cacggtccag cttgttcata aaggtaaaaa
    10261 ctggaatgcc acgatgtttc acaacctcaa acaatttctt ggtttgagcc tcgatcccct
    10321 tggcagagtc cacgaccatg accgcagcat ccaccgccat caaggtacga taggtatctt
    10381 ctgagaagtc ctcgtgccct ggcgtgtcta agatattcac gcgcttgccg tcgtagtcaa
    10441 attgcataac agatgaagta acagaaatcc cacgttgctt ctcgatatcc atccagtcag
    10501 atttagcaaa agtccctgtt ttcttccctt ttaccgtacc agcctcacga atctcacccc
    10561 caaagtagag taactgctca gtgatggttg ttttccccgc gtccgggtgg gagataatgg
    10621 caaaggtacg acgtttctta atttcttctt gaatattcat aagttctctt tctttgattc
    10681 tctatttttc ttgtttcaat agctgagaat gatttttaca ttggatttta ccattccttt
    10741 caacattcca ttatatcgga ttttagcatt tttttcaatt tctatttctt ttcacttccc
    10801 cctcccttat ttataggaaa atatggtaaa atagaacaga ctaaaaatca tcatttcacg
    10861 aaaggatgca agatgaaaat tacgcaagaa gaggtaacac acgttgccaa tctttcaaaa
    10921 ttaagattct ctgaagaaga aactgctgcc tttgcgacca ccttgtctaa gattgttgac
    10981 atggttgaat tgctgggcga agttgacaca actggtgtcg cacctactac gactatggct
    11041 gaccgcaaga ctgtactccg ccctgatgtg gccgaagaag gaacagaccg tgatcgcttg
    11101 tttaaaaacg tacctgaaca agacaactac tatatcaagg tgccagctat cctagacgat
    11161 ggaggagatg cctaatgact tttaacaata aaactattga agagttgcac aatctccttg
    11221 tctctaagga aatttctgca acagaattga cccaagcaac acttgaaaat atcaagtctc
    11281 gtgaggaagc cctcaattca tttgtcacca tcgctgagga gcaagctctt gttcaagcta
    11341 aagccattga tgaagctgga attgatgcta acaatgtcct ttcaggaatt ccacttgctg
    11401 ttaaggataa catctctaca gacggtattc tcacaactgc tgcctcaaaa atgctctaca
    11461 actatgagcc aatctttgat gcgacagctg ttgccaatgc aaaaaccaaa ggcatgattg
    11521 tcgttggaaa gaccaacatg gacgaatttg ctatgggtgg ttccggtgaa acttcacact
    11581 acggagcaac taaaaacgct tgggaccaca gcaaggttcc tggtgggtca tcaagtggtt
    11641 ctgccgcagc tgtagcctca ggacaagttc gcttgtcact tggttctgat actggtggtt
    11701 ccatccgcca acctgctgcc ttcaacggaa tcgttggtct caaaccaacc tacggaacag
    11761 tttcacgttt cggtctcatt gcctttggta gctcattaga ccagattgga ccttttgctc
    11821 ctactgttaa ggaaaatgcc ctcttgctca acgctattgc cagcgaagat actaaagact
    11881 ctacttctgc tcctgtccgc atcgccgact ttacttcaaa aatcggccaa gacattaagg
    11941 gcatgaaaat tgctttgcct aaggaatacc taggcgaagg aattgatcca gaggttaagg
    12001 aaacaatctt aaacgcggcc aaacactttg aaaaattggg tgctatcgtc gaagaagtca
    12061 gccttcctca ctctaaatac ggtgttgccg tttattacat catcgcttca tcagaagctt
    12121 catcaaactt gcaacgtttt gacggtatcc gttacggcta ccgcgcagaa gatgcgacta
    12181 atcttgatga aatctatgta aacagccgta gccaaggttt tggtgaagaa gtgaagcgcc
    12241 gtatcatgct gggtactttc agtctttcat caggttacta cgatgcctac tacaagaagg
    12301 ctggacaggt ccgtacactt atcattcaag atttcgaaaa agtcttcgcg gattacgatt
    12361 tgattttggg tccaactgct ccaagtgttg cctatgactt ggattctctc aaccatgacc
    12421 cagttgccat gtacttagcc gacctcttga ccatacctgt aaacttggca ggactgcctg
    12481 gaatttcgat tcctgctgga ttctctcaag gtctacctgt cggactccaa ttgattggtc
    12541 ccaagtactc tgaggaaacc atttaccaag ctgctgctgc ttttgaagca acaacagact
    12601 accacaaaca acaacccgtg atttttggag gtgacaacta atgaactttg aaacagtcat
    12661 cggacttgaa gtccacgtag agctcaacac caattcaaaa atcttctcac ctacttctgc
    12721 ccactttgga aatgaccaaa atgccaacac taacgtgatt gactggtctt tcccaggagt
    12781 tctaccagtt ctcaataaag gggttgttga tgccggtatc aaggctgctc ttgccctcaa
    12841 catggacctc cacaaaaaaa tgcactttga ccgcaagaac tacttctacc ctgataatcc
    12901 caaagcctac caaatttctc agtttgatga gccaatcggt tataacggct ggattgaagt
    12961 cgagctagaa gacggtacga ccaagaaaat tggtatcgaa cgcgcccact tagaggaaga
    13021 cgctggtaaa aacacccacg gtacagacgg ctactcttac gttgacctta accgccaagg
    13081 ggtacctttg attgagattg tatctgaagc cgatatgcgt tcgccagagg aggcctatgc
    13141 ttatctgaca gccctcaagg aagttatcca gtacgctggc atttctgacg ttaagatgga
    13201 agaaggttct atgcgtgtgg atgccaacat ctcccttcgt ccttatggtc aagagaaatt
    13261 cggtaccaag actgaattga agaacctcaa ctccttctca aacgttcgta aaggtcttga
    13321 atacgaagtc caacgccagg ctgaaattct tcgctcaggt ggtcaaatcc gccaagaaac
    13381 acgccgttac gatgaagcga ataaagcaac catcctcatg cgtgtcaagg aaggggctgc
    13441 tgactaccgc tacttcccag aaccagacct acccctcttt gaaatttctg acgagtggat
    13501 tgaggaaatg cggactgagt tgccagagtt tccaaaagaa cgtcgtgcgc gttatgtatc
    13561 tgaccttggt ttatcagact acgatgctag tcagttgact gctaataaag tcacttctga
    13621 cttctttgaa aaagctgttg ccctaggtgg tgatgccaaa caagtctcta actggctcca
    13681 aggggaagtc gctcagttct tgaatgctga aggtaaaaca ctggaacaaa tcgaattgac
    13741 accagaaaac ttggttgaaa tgattgccat catcgaagac ggtactattt catctaagat
    13801 tgccaagaaa gtctttgtcc atctagctaa aaatggcggt ggcgcgcgtg aatacgtgga
    13861 aaaagcaggt atggttcaaa tttcagatcc agctatcttg atcccaatca tccaccaagt
    13921 ctttgccgat aacgaagctg ctgttgccga cttcaagtca ggcaaacgta acgccgacaa
    13981 agcctttaca ggattcctta tgaaggcaac caaaggccaa gccaacccac aagttgccct
    14041 taaactactt gcacaggaat tggcgaagtt gaaagaaaac tagacagaac aaaaccagcc
    14101 ctaaggttgg ttttttcttc tctaccaact cccaataact attttggctt tatttccaga
    14161 gtattttatg gtaaaatgaa gagtaataat atttattaaa gaggtaaaaa catgattgaa
    14221 gcaagtaaat taaaagctgg tatgaccttt gaaacagctg acggcaaatt gattcgcgtt
    14281 ttggaagcta gtcaccacaa gccaggtaaa ggaaacacga tcatgcgtat gaaattgcgt
    14341 gatgtccgta ctggttctac atttgacaca agctaccgtc cagaggaaaa atttgaacaa
    14401 gctattatcg agactgtccc agctcaatac ttgtacaaaa tggatgacac agcatacttc
    14461 atgaatacag aaacttacga ccagtacgaa atccctgtag tcaatgttga aaacgaattg
    14521 ctttacatcc ttgaaaactc tgatgtgaaa atccaattct acggaactga agtgatcggt
    14581 gtcaccgttc ctactactgt tgagttgaca gttgctgaaa ctcaaccatc tatcaaaggt
    14641 gctactgtta caggttctgg taaaccagca acgatggaaa ctggacttgt cgtaaacgtt
    14701 ccagacttca tcgaagcagg acaaaaactc gttatcaaca ctgcagaagg aacttacgtt
    14761 tctcgtgcct aatctctaga aagaggtcat tctatgggaa ttgaagaaca acttggcgaa
    14821 atcgttatcg ccccacgtgt acttgaaaaa atcattgcta tcgctactgc aaaggtagag
    14881 ggtgttcact ctttttcaaa cagatcagtg tctgataccc tttcaaaact ttcactcggc
    14941 cgtggcgttt atcttaaaaa cgtggacgaa gaactcacag cagatatcta tctctacctt
    15001 gagtacggag taaaagttcc taaggtagcg gttgctatcc agaaagctgt caaagatgcc
    15061 gtccgtaata tggctgatgt agaactcgct gctatcaata ttcacgttgc aggtatcgtc
    15121 ccagataaaa caccaaaacc agaattgaaa gatctatttg acgaggactt cctcaatgac
    15181 tagtccacta ttagaatcta gacgccaact ccgtaaatgc gcttttcaag ctctcatgag
    15241 ccttgagttc ggtacgaatg tcgaaactgc ttgtcgtttc gcctatactc atgatcgtga
    15301 agatacggat gtacaacttc cagccttttt gatagacctc gtttctggtg ttcaagctaa
    15361 aaaggaagaa ctagataagc aaatcactca gcatttaaaa gtaggttgga ccattgaacg
    15421 cttaacgctc gtggagagaa acctccttcg cttgggagtt tttgaaatca cttcatttga
    15481 cactcctcag ctggttgctg ttaatgaagc tatcgagctt gcaaaggact tctccgatca
    15541 aaaatctgcc cgttttatca atggactgct cagccagttt gtaacagaag aacaataaaa
    15601 agaaaaagtg tttgatagat atcaaacact ttttctttgc ctcccactat ctaaaaaaat
    15661 gataatagat ataattgtaa acaaaaatcc agataggttt tgcatgattg agaaagttaa
    15721 aaaaactatg gcagagaatc gttaatctca gattgtcggt agaacgataa acaagggcaa
    15781 aaaagaaacc aatcagacta taatataata aactaattgg atctctgtga gatagtatca
    15841 aacggctaat cccaaagata aggcctgata agatgacatc taaatagtac tttgacttag
    15901 gaaagaaggt attcataaaa taccctctat caagagtctc ctcaaaaaca ggaccgatga
    15961 ttacaagcag gacaaaagat aagatagtcg ataaaaaggt tggttgtcca tttgaaagaa
    16021 ccacggtaaa atactcatca tgaatattcc tatggttaat caaatgagca tagcgtgcct
    16081 aaaaattacc gagaatctga taaaccacat aagttgcaaa taaggagaag acaaatgacc
    16141 agtcccagct ctttttcttt tttaacctcc aaattaatag aaaaggaaac ttcccactaa
    16201 tcccactgtt aaaataagag aatagacatc agctcctaac cctaaaatga tcgtcacata
    16261 caatccaatt gtttgtggta aataggtaga tagtaaaata ataagcaaaa atattccaaa
    16321 ttgtcttagt ttctttgtgt ttctcatcgt acttttttga aagattaccc tgttcggaag
    16381 ccgtacttcc aagcatctat ataagaatta agtgcccctt gcctcatata gggagcaaat
    16441 tctctataat ataaccatct actatatcca tcttcccaaa cagcaagacc acctgaagtt
    16501 tgctccaagt cctcagttga aagaactgta aatgtttctg tacctgtcat tgcaagtacc
    16561 ttcttaaaat agattgttgt aggctcacat ttatagtata tttctttttt tgtctatttt
    16621 atagtctatc tcctcaactg gcaatttttc gacctgaatt gcatttttcc ataaaaaatg
    16681 agacctttct agtctcattt agtcattctt agtattttct aaatcgttga tagcgttctt
    16741 ccagcaactc ttctagcggt ttttgtgaaa gtctagccag ctccgtttgg agttcttttt
    16801 tgacactctt aattagttct ttactagaaa gtcctacttc agaaatcacc ttatccacca
    16861 cgtccatttc taacagttca tgcgaagtga ttttcatcag ttctgctgct tccatggcgc
    16921 gagtaccgtc cttccataaa atggaagcaa agccttctgg actgagaatg gcatagatag
    16981 aattttccag catccagaca cggtccgcga cagctagagc cagagccccg cctgaaccac
    17041 cttcaccgat aataatggcg ataataggaa ctttcaggtc actcatttcc atgagattgc
    17101 gagcgatagc ttccccttga ccacgttctt ccgctccgac accaggataa gcacctgctg
    17161 tattgataaa ggtcacaact ggacggccaa atttctcagc ctgtttcatc aaccgcagtg
    17221 cctttcggta gccttctgga tgtggttggc caaaattccg tttgaggttg tcttgcaaac
    17281 tcttgccttt ttggatacca accactgtta cagcttggtc tccaagccaa ccaataccac
    17341 caacaactgc accatcatca cgaaaagaac ggtcaccatg taattggata aattcatcaa
    17401 aaatgcctgt cgcaaagtcc aaggttgtca agcgactctg ctcacgcgct tctctgacta
    17461 tttttgcaat attcatctag gactccctcc atgcaatctg actaggctag caatcgtatc
    17521 tggtaagtct cttcttttga caatagcatc cacaaagcca tgttctaata ggaattctgc
    17581 cttttggaaa tcctcaggca agctttcacg aaccgtattt tcaatcacac gacgcccagc
    17641 aaaaccaacc aagctctgtg gttcagccag aatgatatcg ccttccatag cgaaagaagc
    17701 tgtcacacca ccagtcgttg gatctgtcaa aatggtcagg taaaagagac cagcatttga
    17761 atggcgttta accgctgcag agatcttagc catctgcatg agactcatga ttccttcctg
    17821 catacgggct ccaccagagg ctgtgaatag gacaactggt aatttttcga cagtcgcata
    17881 ctcaaacaaa cgagtgattt tttcacctac aaccgtaccc atagaagcca tgataaaatt
    17941 agaatccata atcccaagag ccacagtctg acctttaata agagcagttc ctgtcacaac
    18001 ggcttcatgc agacctgttt tttcacgcat agatgccagt ttcttttggt aaccagggaa
    18061 atgcaaggga tccttgcttt caatccctgt aaacaattct ttgaaggttc ccatatcaat
    18121 cgtcaaagcc aagcgttctt gggcagaaat acgaaaggta tagctacagt gcggacagat
    18181 acgttcactt cccagatcct tctgatagat ggtatgctta cagcctggac actgggaaaa
    18241 taattcatct ggaacctctg gcttagcttg aggtttttcc ctaaccgaac gattgggatt
    18301 gattcgaata tacttatctt ttttactaaa tagagccatt gattcccctt ttcggtttaa
    18361 actcttaaag tcattttatt ctttttcttg atatttaggt aagaaggttt ccatcaagaa
    18421 ggaagtatca taatccccag caatgacatt gcgatctgaa atgagatcaa gctggaaatc
    18481 tgcattggtc tgcactcctt caatctctaa ttcatagagg gcacgttgca ttttcatcaa
    18541 ggcgtcaaaa cgattttcgc cgtgtactat gattttggca atcatactat cataataagg
    18601 cggaatggta taacctggat aaactgctga atccacgcgc aagccaactc caccacttgg
    18661 cagatagaga ttagtaatct tacctggact tggagcaaag ttaaaggctg ggttttctgc
    18721 attgatacga cactcgatgg catgaccgcg taggacaata tcttcttgct taacagacaa
    18781 aggctgacct gccgcaatgc aaatctgttc cttaacgata tcaacacctg aaacaaactc
    18841 tgttactgga tgttctacct gaacacgagt attcatctcc atgaaataga aattgctact
    18901 tgcttcatca agaagaaatt caatggttcc tgcattctca tagccaacaa actctgccgc
    18961 tcgaacagca gcagcaccta tttcatgacg cagcgttttt ccgattgcaa tcgagggact
    19021 ttcttccaaa accttttggt tattcctttg aagagaacaa tcccgttcac ccaagtgaat
    19081 cacatgtcca tgctcatcac ctaggatttg aacctcaatg tgccgagctg gatagataac
    19141 ccgttctatg tacatggcac cattgccata attggccttg gcctcactag aggcagtttc
    19201 aaaggcagaa acgaggtcat ctggtttttc aaccttacga atccctttac cacctccacc
    19261 tgctgaagcc ttgagcataa caggatagcc aattttttca gcaacaatca aagcttcttc
    19321 agagttatgc acttctccat ctgaacctgg tataacaggc acacctgctt taatcatctg
    19381 agcacgcgca ttgatcttat cccccatcat atccataaca tgaccagatg gaccgataaa
    19441 cttgatacct acttcttcac acatggtcgc aaatttggaa ttttcactga gaaatccaaa
    19501 accagggtga atagcttctg cctcagtcaa gactgcagct gatagaactg cattaatatt
    19561 gagataagac tctgttgcct tgccaggacc aatacaaact gcttcatctg ccaacagcgt
    19621 atgaagagct tccttatcag cagttgaata aaccgctacc gtcgcaatcc ccaattcacg
    19681 tgccgcacgg ataatacgaa ccgcaatttc accacgattg gcaattaaaa tttttcgaaa
    19741 catggagaac ctccttagtt cccaattgca aaagtaaggg taccactggc tgcaagcttg
    19801 ccatccactt cagcctttgc ttcaaccaca gctatggtgc cacgacgttt tacaaaagtc
    19861 gctgtcataa ccaattggtc gcctggtaca ac
//
LOCUS       DAVBHS010000023        19617 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVBHS010000023 DAVBHS010000000
VERSION     DAVBHS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19617
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.023"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..789)
                     /locus_tag="VQ651_000579"
     CDS             complement(<1..789)
                     /locus_tag="VQ651_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HET2753609.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETAR"
     gene            complement(790..1563)
                     /locus_tag="VQ651_000580"
     CDS             complement(790..1563)
                     /locus_tag="VQ651_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET2753610.1"
                     /translation="MKSKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLASFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(1553..1831)
                     /locus_tag="VQ651_000581"
     CDS             complement(1553..1831)
                     /locus_tag="VQ651_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HET2753611.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGKGKISLEK"
     gene            complement(1831..2232)
                     /locus_tag="VQ651_000582"
     CDS             complement(1831..2232)
                     /locus_tag="VQ651_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HET2753612.1"
                     /translation="MITLFLSPSCTSCRKAKTWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(2442..3059)
                     /locus_tag="VQ651_000583"
     CDS             complement(2442..3059)
                     /locus_tag="VQ651_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HET2753613.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(3049..3822)
                     /locus_tag="VQ651_000584"
     CDS             complement(3049..3822)
                     /locus_tag="VQ651_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HET2753614.1"
                     /translation="MKYKGYLIDLDGTIYKGKDQIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(3833..4570)
                     /locus_tag="VQ651_000585"
     CDS             complement(3833..4570)
                     /locus_tag="VQ651_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HET2753615.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEESISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(4575..5705)
                     /locus_tag="VQ651_000586"
     CDS             complement(4575..5705)
                     /locus_tag="VQ651_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HET2753616.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(5789..6178)
                     /locus_tag="VQ651_000587"
     CDS             complement(5789..6178)
                     /locus_tag="VQ651_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET2753617.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(6194..6577)
                     /locus_tag="VQ651_000588"
     CDS             complement(6194..6577)
                     /locus_tag="VQ651_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HET2753618.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(6578..7366)
                     /locus_tag="VQ651_000589"
     CDS             complement(6578..7366)
                     /locus_tag="VQ651_000589"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET2753619.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(7359..8294)
                     /locus_tag="VQ651_000590"
     CDS             complement(7359..8294)
                     /locus_tag="VQ651_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HET2753620.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLSETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(8424..8600)
                     /locus_tag="VQ651_000591"
     CDS             complement(8424..8600)
                     /locus_tag="VQ651_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET2753621.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(8740..9447)
                     /locus_tag="VQ651_000592"
     CDS             complement(8740..9447)
                     /locus_tag="VQ651_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HET2753622.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            9598..10626
                     /gene="queA"
                     /locus_tag="VQ651_000593"
     CDS             9598..10626
                     /gene="queA"
                     /locus_tag="VQ651_000593"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET2753623.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIINMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDSMFIY"
     gene            complement(11297..11686)
                     /locus_tag="VQ651_000594"
     CDS             complement(11297..11686)
                     /locus_tag="VQ651_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2753624.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(11740..12447)
                     /locus_tag="VQ651_000595"
     CDS             complement(11740..12447)
                     /locus_tag="VQ651_000595"
                     /inference="COORDINATES: protein motif:HMM:NF019142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2753625.1"
                     /translation="MFRRNLNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGGIIVK
                     VATKPKVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTE
                     QVTVDRKQATDTVIKVPAKSKVEEVLVPCATKYEADNDLSAGQEQEITLGKNGKTVTT
                     ITYDVDGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDDPTLDKSQE
                     VEEVGEIGKLLLLQSIL"
     gene            complement(13147..13698)
                     /locus_tag="VQ651_000596"
     CDS             complement(13147..13698)
                     /locus_tag="VQ651_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2753626.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDIEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(14096..14920)
                     /gene="nadE"
                     /locus_tag="VQ651_000597"
     CDS             complement(14096..14920)
                     /gene="nadE"
                     /locus_tag="VQ651_000597"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HET2753627.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(14917..16377)
                     /locus_tag="VQ651_000598"
     CDS             complement(14917..16377)
                     /locus_tag="VQ651_000598"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET2753628.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(16493..16981)
                     /locus_tag="VQ651_000599"
     CDS             complement(16493..16981)
                     /locus_tag="VQ651_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753629.1"
                     /translation="MKNRFYYYQLLDEREEQLINKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(16971..17180)
                     /locus_tag="VQ651_000600"
     CDS             complement(16971..17180)
                     /locus_tag="VQ651_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2753630.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(17570..18012)
                     /locus_tag="VQ651_000601"
                     /pseudo
     CDS             complement(17570..18012)
                     /locus_tag="VQ651_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640911.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18304..19110)
                     /locus_tag="VQ651_000602"
     CDS             complement(18304..19110)
                     /locus_tag="VQ651_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2753631.1"
                     /translation="MILKDINFALNKGEIVGLVGRNGVGKSTLMKILVQNNQPTSGNI
                     ISSDNVGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQERFRSLIQELDLTQSINKKV
                     KTYSLGTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLAVLKKLALNENVGILI
                     SSHKLEDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGD
                     IVQEEGLRITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNRSK"
     gene            complement(19516..>19617)
                     /locus_tag="VQ651_000603"
     CDS             complement(19516..>19617)
                     /locus_tag="VQ651_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2753632.1"
                     /translation="VPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
BASE COUNT         5876 a         4366 c         3419 g         5956 t
ORIGIN      
        1 ccgagccgtt tctggcaggt caataccagc taccattcca ttgatatgct ctactggcaa
       61 caagtcaaaa tcatactctt ccagcaacca attgacaatc tcttcgtttt cctcgggtgc
      121 ccaggtacag gtcgaataaa ccagacgacc accttcagct aacatggtca ctgcatcctc
      181 cagaatttct ctttgcaagc tagcacattg actcggataa tctaagctcc aatagtccat
      241 agcatcaggt tgcttacgaa acattccttc accagagcaa ggggcatcaa gaacgattaa
      301 gtcaaaatag cctttaaaga ccttgaccaa gcggtcggca gattcattgg tcaccacgac
      361 atttgtcgct ccaaaacgct ccatgttttc aaccaaaatc ttagcccgtt tgcttgaaat
      421 ttcattggaa acaagtagcc cctcccctgc tagataggct gccagttgag ttgatttgcc
      481 ccccggtgca gcagccaagt ccaagacctt cataccagga ctgggttggg ctacttgagc
      541 caccatttga gcagcaggtt cttgcgaata aactaaacct gtagcatgct caggcgattt
      601 ccctgaaacc ttcccatagt ggccccaagg ggtttgagta atggcatcag aaaaggaaag
      661 ttgctcttct tttaagggat tgacccgaaa ggccgaaacc gcttcctcct caaaagaggc
      721 aagaaaatct cttgcctcat ctcctagtat ttctttatat ttttcaacaa atccttctgg
      781 aaatttcatt taagttcttt tcctttcgta aatataggac tgaatttcct cctgcatctc
      841 aagaggcacc atcatgaccg gctgtctggt ttgaaaatca ggagcttcac caaaaagggt
      901 cacaacccga tagcccagac tttcccctaa aatactagct gcggcataat cccatggttg
      961 cagataggtg agataggtca acaaacgccc tgacaaaatc ttggcaaaac taatggccgc
     1021 acttccatag acacgaacac caagaaccgc tcggctcaaa tcagccagcc cccattcatt
     1081 ggtttccagc ataccactat tccctgcaat gagaaaatct ccaagtggtt tagttttaaa
     1141 agaagctagg ggcctatcat ttagacaaac tggaaattcc ccaccaccgt ggtaacaatc
     1201 ccctttgacc acatcataaa tcagaccaaa ctgtccctga ccattttcaa aataagccat
     1261 cataacagca aaatcttcct gctgggctac aaaattattg gtaccatcaa tgggatcaat
     1321 gacccaaacc ttgccctctt gaaccgaggc tcgcagacaa ccttcttcag cacaaatctt
     1381 atcctcagga taacgggaca aaatctcacc aaccaagagt tcctgaactt ctttgtccag
     1441 tctggtcacc aaatctgttg gagaggactt ggtttcaaca cgcaagtctt cctgcatatg
     1501 gtcaagaatg tactgacctg ctttcttaac aagctcttta gcaaattcaa atttactttt
     1561 caagagaaat ctttcctttc cctttttctt tggcagcctg aactgctcgg taaagtgaat
     1621 agccactaac cgtttcaaat tctcgtccca aacgtttctc ttcgctcttg cttggcacga
     1681 ccgctttgaa tcccttatag gagtccagta actttttagc ctctactttg ccttcatagg
     1741 cagcttcaac atcattaaaa aaagaaagca ctgaagcaag ttcttcagtg ctccacgaca
     1801 aatctagtgg gtaactatac tgtttgttca ttaactaata ccagctctca ttcttgcttc
     1861 ttttagttct tgcttacgat aactacgagg gagaaaagca cgaatctcat cttcattaaa
     1921 accgatttgc atacgcttgg catcaataat aattggacga cgcaaaagac taggatactg
     1981 ctcaatcaaa tgaagcaatt ccgataccga aatactctct acatcaatat tcaatttttg
     2041 aaaaattttt gaacgagttg aaatgatgtc atcagtacca ttttcggtca aggaaaggat
     2101 gtgttgcaat tcttttcttg ttaaaggact ggtcataata ttgtgttcca caaagggaac
     2161 cttatgtttt tctaaccagg tcttagcctt acgacatgat gtacagctcg gtgatagaaa
     2221 tagtgtaatc atgcttttct cttcttatct atactttgct acttctatta tacaaaaaaa
     2281 taaggcgctt gactagggat ttttagaaaa aaagcctatt ttttcaggaa aaataggctg
     2341 tttgcgaacg attgacacaa ttggatttgg ttaattcact cttaacgatg gttttaaacg
     2401 atatatatat ttatatatgt aaattaaaaa catctttcct ttcacttcct acgacttttc
     2461 agacacagat agccaaagaa gctttcatag agggcaaaaa agaggaggaa ggcatgaaga
     2521 aagaaggtct ctggcaaaat cataataaca ggatccttgg ctggatcaaa aagccaggta
     2581 tcatctccca caaagagaat ttgatggaaa agagtaaaga attggtcaaa accaatcaaa
     2641 actcccccaa gtccaatcat cacaggtaag actactagag ccaggagact ttttcgataa
     2701 agagacaaaa agtccttttt cacaatccta ttgacaaaga catagaaact tggcagtgtc
     2761 actagagcta ctagctgaac caaatgaaag agattcttga ccactgcgaa atggtgcaaa
     2821 ccagctgctg acgaacgaaa atcaggcatc tgtaagacct gactaaaagg attggtcaga
     2881 taattcatca agatatgaaa attgtattga atggtttctg gttttagata gactcgattc
     2941 gttaagttta gccactgaat ctccatagga tagaaaatcc aagccagata aatggtcaga
     3001 aggattgaga gggagaggag aaagagcata gagccccaaa aggtcaattt agttttcatc
     3061 aaaatcccac tccgcaaggc tagaaaccac atgtgtcggt gcgattggca ggccagctac
     3121 ttcttctgcc ttggtaaaac ctgtcgtcac caagagcgtt ggaatgccat tgtcaatccc
     3181 agcccggata tcagtcaaat aattgtcccc aaccatgatt aactcttcac gttccaaacc
     3241 taagtgctca actgccttgt ccataatgat ggcattgggt tttccgatat aaaccggctt
     3301 cactcgtgtc gctacttcaa gcagcgtaat cagtgagcca gcacctggca aaagaccacg
     3361 ttccgtcggg atgttgaggt caggattggt tccgataaaa tgggcaccct tttgaatagc
     3421 aagagttgct gtggcaaatt tttcatagtc gacttgccaa tccagaccaa ctaccacgta
     3481 ggcaggtttt tccttgtctt ccacataacc agccgccttg atggcttcct tgagtcctgc
     3541 ttctccgaca acatagacgg tcttttcaag ccccaaatca ttcatatagt cgatggttgc
     3601 caaagtcgct gtgtagacag tcgatagggg cgtatcgata ttaaaattct gagccaacat
     3661 ctccttaaca ctctctggag tgcgggttgt attgttggtt acaaagagat agggaatgtc
     3721 ccgcttttgc aattcatgaa caaaagtctc tccagcaggg atttggtctt tccccttata
     3781 aatggttccg tctaaatcaa ttaaatagcc tttatatttc atctatttct ccctaagcct
     3841 tttttatttc ttgccaagta atgattgctt gggcattgat aaccccatca cttgtaattt
     3901 catgcttgct ttccagtcca gtccgttcaa cagccgatgt aatcacccca cctggtcgaa
     3961 cttccttgac atacttgagg ttgattttct tgggaatata gtgggtcaaa aaatccgctc
     4021 ccatgacctc aaaaatccag tctaagtatt tactgttatt gacatgacca ttcatatcca
     4081 agtcgtaaaa acgaacatgg taatccttgc tgatcgactc ttccaaggac tcatacttcg
     4141 gtccacggat aagtttttta tcaaaatcag actggtaagg agccacaatc tcaggttcaa
     4201 cagcatggac ttttcgactg tcgcggtcca tgagaacaaa ggtcgccatc atatggatga
     4261 gctcctgccc cgcttcatta taaatagtaa agcgacggta gcaaaaaagt cgattgtagc
     4321 tcaaggcttc cgtttcgatg gtaatttctt ccgcaaaacg aggcaaacga accacctcaa
     4381 tatcatattc tacgataatc cagaccagat tatagtcttc caaaatggcc ttatcactaa
     4441 ctcccagttc aatcgactgc atccctgaaa cttgcagtga cagcaaaatt acatctggaa
     4501 gtttgatatg accgttcata tcagccatat caaaaggaat tttcattttc atttgataag
     4561 ttaagcccat gatcctactc caaaataaat cgttccgcta cagtatctcc caaaaagaga
     4621 cctctctttg tcatgcgaac gtggtcaccc tcaatctgca tgaggccttg ttgaaccaaa
     4681 tctctgacaa tttctccata aagtccagca aaagactgtc caaatttttc ctcaaatcgc
     4741 gccatggaaa ccccggattt cttgcggagt cccaagaaca tttcttcttc catttgctcc
     4801 ttttgactca ggtgatcttc tgtaatacaa gcattgcctt cctcaaccgc actgagataa
     4861 tgacgaatgg gaccatgatt tttatagcgt actccattga cataaccaga tgcccctgca
     4921 ccaataccat agtattcagc attgtcccag tacatgagat tgtgacgact ttcaaagccg
     4981 ggtttggaga aattagaaat ctcataatgc tcaaaacccg ctcgctccag ctctgcaatg
     5041 atgtactcaa acatctccgc ttctagttcc tccttaggca gaggcaattt cccacgtcgc
     5101 atccgattca taaagaccgt atggttttct aaaatcaaac tatacaaact catgtgggga
     5161 atatccagtc caatggcttt agccacattt tcctttactt gctccatggt ctgaccaggc
     5221 agagcataaa tcaaatcaat ggagatgttg tcaaaaccag ccagtttcag gcgatcgata
     5281 ttttcataaa tatccttctc caaatgactg cgcccaatct ttttcaacat cttatcatca
     5341 aaggtctgga cacctagcga aacacgattg acagccgaat ttttcaaaac agctatctta
     5401 tccgcatcca aatcgcctgg attggcttca atggtcaact cttctaagac agacaagtcc
     5461 aagtttttag tcaagccatt cagtaaaacc tccagttgcg gagccgacag ggctgtcggt
     5521 gttccaccac cgatataaag ggttgacaac ttttcaatat cataagaacg aaactcttcc
     5581 agcagatgct ctaaatagct gtcgactggc tgatttttga tgaagacctt tgaaaaatca
     5641 caataataac aaatctgggt acaaaatggg atgtgcacat aggctgacgt tggttttttc
     5701 tgcatagtaa ttattatacc acaaagacta gattccagat aaaaatcacc atccccagat
     5761 acatagtccg tccggagatg gtgatggttt attcttctgt tatatcaatc acaatctctt
     5821 ctgagtcatc aagagcttcg gctttttctt gccattgctc cttgagatta tttaattgat
     5881 tttttgatgc ttctgtcgct tgaaaagcat aggatttagc ttgagcaagt atactgtcca
     5941 cagtgatttc acctgactca acctgttctt ttgttttcag aacaaaatct gtagcctgct
     6001 ccttaacttc tgtcagtttt tcacagactt gctccttggc atactccgga tcttctctca
     6061 aatcatctag aaaatcttga gcctgactgc aaacttgttt gcccttatca cttgttaaaa
     6121 acaaggcaag agctgcacct gaaacggttc ctaaaaggat tgaggataat ttacccataa
     6181 ggattctcct tttttatttt ttgaaaaatt tacttgcaag acgaagagct gacagacttg
     6241 caccagtctt gagtgttttt gaaccagctg atgaagcttt cttgctcaag acacgcgcat
     6301 ggtcattgag gtctgaaaca gatagagata aatctgcaac agcactgaag agtggatcaa
     6361 tcgtagccac cttgacattg atatcatctg ccaagacatt gaccttagcc aacaactcat
     6421 tggtgtgatg caaggtcaca tccacatctg aagtcaaggt tttaatcgtc ttttctgttt
     6481 catcgatgac acgaccaagc ttttgtacag taatgatcag atagaccaaa aagacaatca
     6541 aagctagggc aacaagaata tatgcaactt ctaacattta gttttcctcc tctgtaatat
     6601 agtaaggggc cttctttcga ttttgataaa taacgatcat tataccgaga ccgataagga
     6661 caactgacag ccattgggac actcgaaggc cgaagaacat gagactatct gttcgcatac
     6721 cttcgatgac catacgaccg aaaccatacc aaatcaagta aaaggccgtg atatgacctc
     6781 gtctgagact cttccatttc cgtctaaaaa tcagaatcaa ggcaaagcca agcagattcc
     6841 atagagactc ataaaggaaa gtcggttgac ggtagctccc ctcaatatac atctggtcac
     6901 ggataaagcc aggtagataa tccagattat ccactgttgc accataagct tcttggttaa
     6961 agaaattacc ccaacgcccc aaactttgag caatcataac gctaggcgcc gcaatatcta
     7021 gaaaatccca agtattgatg agtttacggt cagcaaagat atagagcaca agagccccag
     7081 ttatcaaacc accgtaaatg gccaaaccac cattccaaat ggcaaaaatc tctcctaaat
     7141 tctgactata gtaatcaaat cggaaaataa catagtagag acgagctcct aaaatagcca
     7201 agggaaaggc tactaagata aaatctaaaa tatcgtctgg tatgatcttc tttctaggtg
     7261 cttctttcat ggtcaaataa accgcaagaa tcaagcctgt cacaatacat aaggcatacc
     7321 aacgaatggc taggggtcct agttgaatag caattggatc aagcattttg cacctcattt
     7381 cgagcgatga gacttgtcag tcgttcgtcg aacaaacggg tcgcatcaaa gcccatttcc
     7441 ttggcacgat aattcatggc agctgcctca atcacaacag agatattacg acctgtttta
     7501 actggaatac gaatacgagg aatggctacg ccagaaactt caagttcctc tgcattgttt
     7561 ccaagacgat caaaggtctt atgcgtatcg taattttcca aatagacagc aagctgaacc
     7621 tgtgaagaat ccttgacagc actcgcaccg tagagactca taacatcgat aataccaacc
     7681 ccacgaattt caatcaagtg tttcaaaatt tcagctggtt caccccagag agtaatctca
     7741 tccttggcaa agatatcgac acggtcatct gctaccaaac ggtgaccacg tttgacaagc
     7801 tcaagacctg tctcgctctt accaattcca ctatctccct gaatcaagac gcccatccca
     7861 taaatatcca tcaagacacc gtgcacactg gtacgttctg ccaaacgaga atccagatag
     7921 ctagataact ctccagataa acgactggta gctgcacggc tggttaaaat agcaatctta
     7981 cattctctag cagcctttaa catctcctct ggaaccacca aaccacgggc aacaatgact
     8041 gctggtgtct cagatagaaa catttttttc aaaacttcat aacggctgtt ggaaggcatg
     8101 ctgatcagat aagaccactc cttcatcccc aaaagttgaa tccgctctgg tgtgtagtag
     8161 tcaaaatagc ctgtcatttc aagaccaggt cgcgtaatat ccgctgtatt gatttccttt
     8221 tcaagcaact ctggttcacc atagacaata tctagtctaa gcttttcaat cacttctttt
     8281 actaaaaccg acatcatttc ctccttcaaa tgagttcttc tctctattat atcaaattgt
     8341 aggaggaagt ttcagatttt tgcaggcttt ggagtattta tttaaaagaa aaaggactag
     8401 atttctctag cctttcattc caattagaat tttttacgtt tacgagctac ttctgattta
     8461 cgtttacgtt ttacagaagg tttttcatag aattcacgtt tgcgtgtttc ttgaagagta
     8521 ccagctttag taaccgcacg tttgaaacgg cgaagtgcgt cgtcaagaga ttcattctta
     8581 cgtactactg ttttagacat ttttttcact cccttcaagt tcgatatcta tttcattcta
     8641 cacgtaaaag gaataattgt caagcaaaaa tgctgtttaa ttttgatttt ttcttccatt
     8701 tatacaagag ttgaaagcgt tcaatagaac attgcttttt tatttttcga gtaagctaag
     8761 cgcttcagca tctgcgatga tggttacatc agggtgattt tggaggctac ttgcaggtag
     8821 gttctcagtc actgggccag atactgttcc ggcaatggct tctgctttcg actcaccgta
     8881 agcaaaaaga ataatagact tggcatccaa aatgttttta atccccattg aaatagcttg
     8941 ggttgggacg tcttcaatct tggcaaagaa gcgtgcattg gcttcgatag tagactggtc
     9001 aagttctact agatgcgttt gactgtcaaa tggagtacca ggctcattaa agccgatatg
     9061 cccattgcga ccgattccca agatttggag gtcaactgga tggtcagcca aaatttggtt
     9121 gtagcgttca acttcagctt cagcattgtc cttaacacca cgaggcaaga aactttcttt
     9181 aaatggtttt tggttgaaca agttttcttg catgaagtaa cgataagact gtggattgtc
     9241 cccatcaagg cccacatact catcaaggtt gacactggtt agatttgaaa aatcaaggtc
     9301 actctcaaca atttccttgt aaaattcaag tgggctactt cctgtcgcaa gtcctagagt
     9361 ttgagctcca ttagccaatt tttccttcaa aatctcaaaa gcaacttttc caccttgaac
     9421 ttggttttca actttaataa ctttcatctt ttcatcctcc atttctgtct atatttatta
     9481 tatggtatag accaattttt gtcaagtgaa aacgatttaa tttctaaaaa aagagcgtcc
     9541 aaacttgaaa cgtgttataa tggataggat tagaacttag aaagaatgaa cagatatatg
     9601 aatacagctg attttgattt ccacttgcca gaggaattga ttgcccaaac gccccttgaa
     9661 aaacgtgatg cctccaaact cctcatcgtc aaccgtgaga caggagaaat gcaagataaa
     9721 cacttccact ctattattaa tatgctggaa cctggtgatg cccttgtcat gaacgacacc
     9781 cgagttctcc ctgcccgcct ctatggtcaa aaagtggaga caggaggtca tgtggaactt
     9841 ctcctcctta agaacactag tggagacgag tgggaagttc tggctaaacc tgccaaacgc
     9901 ctcaaggtcg gtactcgtat cagctttggt gatggccgcc tcagcgctgt cgttacagaa
     9961 gaattgaccc acgggggacg cattgtccgc tttgaatacc aaggaatttt cctagaagtc
    10021 ttggaaagtc tgggagaaat gcctctgcca ccttatatcc acgaaaaatt agatgaccgt
    10081 gaacgttatc aaaccgtcta cgccaaggaa agcggctctg ctgcagcacc gactgctggt
    10141 cttcacttca ccaaagaact gctggcagaa atccaagcta agggtgttca tctagtctat
    10201 ctgactctcc atgtcggact cggaaccttt agacctgttt ctgtggataa tctggacgaa
    10261 cacgaaatgc attcagagtt ctatcaactt tctgaggaag ctgctgccac ccttcgctct
    10321 gtcaaagaaa atggtggtcg cgtcatcgct gtcggaacca cttctatccg caccttggaa
    10381 actattggtt ccaagtttga tgggcaaatc caagcagatt ctggttggac caatatcttt
    10441 atcaaacctg ggtatgagtg gaaggtcgtg gatgccttct caaccaactt ccatctgcca
    10501 aaatcaactc tggtcatgtt ggtttctgcc tttgcaggcc gtgaattagt cttagatgcc
    10561 taccaccatg ccatccaaga acactaccgc ttcttcagtt ttggtgactc catgtttatt
    10621 tattgagaaa gaatttctct aaatcttcta ataccaataa atcgctaaga tattatttca
    10681 aagaacatct acaattgaaa ctctagctag ctgtagaaga cgcctagtac attgaaatta
    10741 aaatgcttcc ccctagcttc gaaaatattg ccatagattg cgttgactct ccaaattgat
    10801 tcatctatat tttatttcag cttcctatat tttcttcgct gtttgtaaat caaaatgcaa
    10861 gacacatgag tagcaccata tttgttactc ttatctgtcc tctcaagaga ctattatgag
    10921 ttatttcaga atcattcact actttgaccc tgactctcct tagtctcaaa atcaaagact
    10981 tatactcttc aaaaatctct tcaaactgcg tcaacgtcac cttggattat atatgtgact
    11041 gacttcgtca gttctatcta caacctcaaa gcagtacttt gagcaacctg cgactagttt
    11101 tctagtttgc tctttgattt tcattgagta ttaaacaaaa agtgaacaaa tctgaattct
    11161 aatgtacaga agactaggct tgttcacttt tttatagtcg ctataagatg accttatcta
    11221 tagcttttta tatataatta tatataatta tatattcaga catactatta tcaattttgt
    11281 cgcagggagg aatctgttaa cgcacccatt caccattatc attgactcta tagccatcta
    11341 tacttgtatt gaccgctaac tcacccgatg catttacata ataccattta ccaccaactt
    11401 ggaaccattg attgactttc atagaaccgt tgctgttgag gtagtaccat gaaccattaa
    11461 cttgtaccca acctgttgcc atggaaccat cagtattata aaaataccac ataccatttt
    11521 cttgtttcca gtctgttgtt ggagcaactg ctttagctgg ttctactgct acatctgttc
    11581 cttggttaga tgtaacagat acaggatacg aaggaataga tgattgctca ggaacaacaa
    11641 ctttttcagg ttctctcgtc cctctcctta tacgtctttt taccatctct ttagtaattt
    11701 gacgagaagt agtttcttca attgttccat cacgttcatc tacagtatag attgtagtaa
    11761 gagtaattta ccaatttctc ctacttcttc tacttcttga cttttatcaa gagttgggtc
    11821 atcgagatat tctgtttcga ttggaatttc ttggacaaga acttggggct tggttccttt
    11881 tttaacaact cttgtttgag agtcttcttt ttgacttaaa gtactctcag ttacttgtcc
    11941 actctttcca tctacatcat aagttatcgt tgtaactgtt ttcccattct ttcctagagt
    12001 aatctcttgc tcctgtcctg cagaaaggtc attgtctgct tcatatttag tagcacatgg
    12061 aacaagaact tcttcaacct tgcttttagc tggaactttg ataactgtat ccgtggcttg
    12121 ttttctatca acagtaacct gttcggtaac atgaccagtc tctggattaa catcgtaggt
    12181 ccttgtcgta gttacatagc catcctctcc atcaattgta acaggatttt cactacggtc
    12241 ttttgtttca tctttttcat aacgaattcg cgtacttgaa attttcttgg ttactacctt
    12301 aggtttagtc gctactttta caataatacc cccattgtca gcgtcatcat actctattcc
    12361 ctcttcttta tctctagtat catctctgac atattgaatc ccatcagcag catgaacaaa
    12421 acttgtattc agattcctcc taaaaataaa gttagcccga ttaccgcaga accaaaaatc
    12481 tttccgagtt tacgtaatgc atagcgctta ttagtattag attttgccat tacatcctac
    12541 ttctagtata gcatcttttc tatcaaacgt taaacaatat actttatata taaaatagac
    12601 ttagaatgat atattgatta ttgaactaac actttaacta tatcgtaatc aatctcatat
    12661 ataaaggatt gcagacatct tatctaaata catgctaata tatttagata caaacattcc
    12721 aacttgataa ttttcactca tctttcatca ttccttatac aactatgcag tataaataga
    12781 atagttttct catcagaatg agactatttt aatattagat aatttgatag aaaaaacatg
    12841 aataaaattg gttgaattta attccctaac tcgatagagg attctatttt aaatttaaca
    12901 acagctagga ttacactata aaaagtacat taccacctgt tcggctggag aatctagtcg
    12961 attagctccc tcttagttac aagcttaaag cgatttttta actaatacta aaccagatta
    13021 aagaaggaac tagaacatct gtcttgtact aatcaatatg ctattttaaa gtttcaaata
    13081 aacttggaat gctaattatt atatttacaa taaatagttg acttcgatag aaacagaaaa
    13141 tagaattcaa cattcttccc tacttatacc ataataaata agatccgcaa gatagccttt
    13201 tctctctaca ccattaacaa tggtttgtag ataggacatt ccagcctttt ccatgacacg
    13261 acctgaagct gggttgagac tggcataacg tgctctgacc ttttgaaaac ctgcttgagt
    13321 aaaacaaaag tccaagatag ctttcaaagt ctctgtcatc ataccatttc cccagtaagc
    13381 cttgcctaac acatagccaa tttcacagct taaatcagcc tcgtctatct taacgatgct
    13441 gatatctcct atcacttgct ctgggttttc ttttagacaa atggcccatt tataatagtt
    13501 gagattagta taggaagcaa cccaattgcg aatcgagttt cgagtgattt cgatatcagg
    13561 atggggatcc caggtaacat aggtcagatt ctcagcagat gaagcccaat tttgaaacat
    13621 ggcttctgca tcactctcca caaatcttct taaaatcaaa cgttctgtct gtaatatttg
    13681 cgtaccgata gatttcatga tgctacctca cttccttatt tctatgctcg tagtaaaaag
    13741 gctcaactca gtaacagcta gaaaacgagt ttgtcttcta atttcttgac gcagtcgctg
    13801 cctggtttga aatgcgctct agcaagcttt ttcaaaccta gtcatcgttg caggggtaag
    13861 acgatgaaat cgaactcttc gagttaccga atctgcctca ttttcttatt tctaaactcg
    13921 gggttcaaga tcccccagac cttctctcac tttcttattt caagatgaga ggctgaaaac
    13981 ctccagtgga ggtttttctt gctttttaat ttcagggcaa gaagctaaaa aggtccccgg
    14041 accttcttcg ctttctcatt tctaggctca gggtaaaaac ctccaatgga ggtttttact
    14101 cccaaaagtc atcaaataca gtgatgggta agtggcgttt gtgttggcct ttgtgccacc
    14161 agttttcaat ggtcgcttga gcttctgggc tgattgtttt gccttctagg tagtcgtcaa
    14221 tctctgcgta ggtgactcca agtgcgactt cgtcagctag gcctggttta tcttcttcta
    14281 ggtctgccgt tgggattttt tcataaaggg ctggctctgc gccaagtttc tgcaagagct
    14341 gttttccttg gcgtttattg aggcggtaaa gagggagaat atccgcaccg ccgtcaccaa
    14401 acttggtaaa gaaacctgtg atattttccg cggcgtggtc tgttccaatg accgctccgc
    14461 tatgggaacc agcaagggca tactgagcaa tcatacggca acgtgccttg atattcccct
    14521 tgttgaagtc tgaaacagga ctacctgtcg cttcaactgc agctgtcatg gcatcagctg
    14581 attccttgat attcacaacc aagctgacat ctggctggat gaaggctagg gctttttgag
    14641 catctgcttc atcagcttgc actccgtatg gcaggcggac agcgataaat ttgtagctgt
    14701 cgtctcccgt ttcagctcgc agttcttcca tagctaattg cgccaaacgc cctgccaagg
    14761 ttgagtcttg tcccccagaa atccctagta caaaggtttt taggaaggga tgttttttca
    14821 gatatctttt taagaaatca atagaacgac ggatttcttc ctgggcatca atcactggtt
    14881 tgacacccag ctcttggata atcgtttctt gcaaactcat tcttcttctc cttcaccaag
    14941 ggcttccttg cgcatcttat caatcaagtc catcttatct tgccatacat cacgcgccaa
    15001 atccactgga tagtgctgcg gattgagcac acgcttatac tcatcccaca acttgtcaaa
    15061 ttccttacgg gcataatcct gaatgtcagt caaactaggc aagttgtaaa ctaatattcc
    15121 ttctttgaag atatccacca agagaggaac ggcatcaaaa ttacgaaccg tcttcttgat
    15181 gtatgtatag gtcggatgga acatcttgat ttctgtcatg tcgctaatat ccacaccatc
    15241 ataagtgata tagtcgcctt ctgacttgcc tttttcacga ctggtaatgc gccacacctg
    15301 cttcttacct ggcgtagaga ctttttccgc attattagac agcttaatcg tattgcgcat
    15361 ctgaccagtt tcatcttcga ttgcaacaat cttgtaaact gcaccaagag ctggctggtc
    15421 ataagctgta atcagcttgg tacccacgcc ccagacatca atcttggcct tttgcatctt
    15481 gagattgagg atggtatttt catctagatc attagaagca taaatcttag cctctgtaaa
    15541 tccagcctca tccagttgct gacggacttt cttagaaatg taggcaatat ccccagagtc
    15601 aatccgcaca cccataaagt taatctgatc acccagctca cgcgccacct gaatggcagc
    15661 tggtacaccg atgcgaaggg tgtcataggt atccacaaga aagacacaat ttttgtgggt
    15721 cgcagcgtaa gccttgaaag cttcatagtc attgccataa acctgtacca aggcatgggc
    15781 atgggttccc aaaacaggaa tgtcaaagag cttacccgca cgcacgttgc tggttccatt
    15841 ggcgccacca atcacagctg cgcgtgttcc ccagatggcc gcatccattt cttgagcccg
    15901 acgtgtccca aactccatca agggttcatc ttcgataacc gaacgaatac gagctgcctt
    15961 cgtcgccacc aaggtctggt agttgacgat gttcaaaaga gccgtttcga ccaactgaca
    16021 ttgggctaga ggtccttcca cctgcacaat cggttcatta gcaaaaacca aatccccttc
    16081 ttgggcagaa cgaacggtca actccaactt gaaattgcga aggtaatcca agaacgcccc
    16141 atgataacca agcgactcca aataggctat atcactatct gaaaaacgca agtcttcaag
    16201 atagttcaca attctttcca aacctgcaaa aaccgcatag ccgttcttaa aaggctgttg
    16261 gcggaaatac acctcaaaga ccgccttctt attgtgaatc ccttggtcaa agtaaacctg
    16321 catcatgttg atctggtaca agtccgtgtg caatgtcaaa ctatcatctg gatacatact
    16381 tttcctactt ccttagctag aaacccatga aaattttcaa gaactttcat gtattccaat
    16441 aaattagtac tattatatca cattttagct ggattgagaa aagagtaaca agctattctc
    16501 cactctccaa ttcatccata tcttgttcaa attttttctg agcccattcg ccatagctct
    16561 taagaccaag attgccaata aagacccacg gaaggtaaat gacataagta atgacccaag
    16621 cagacaggta tttaaaattc aaaggattgt gctgataaat ttctatgttg aattgataat
    16681 tctgcaacat caaaagagcc gtaatagcca aggttaggaa aaaacaaccc aaaatcgtaa
    16741 aatgaaaacg actatagtag gtcactccca gataacgggc acggttaaaa aagtaaaatg
    16801 tccctatgat gataacgatt agcagcatat tagaattaaa aaggcttggt gctaatactg
    16861 aaatgatata agataggagc gacaaagcga tgcagatata gaaactttca gagcccgctt
    16921 tattgatcag ttgttcttct ctttcgtcta gtaattgata ataataaaat ctatttttca
    16981 tcttcttcct cccaaaatag ttggtctagg gttttcccta aacatctgca aatagactgg
    17041 cagagcgaga gactgggatt gtattttccc gcctctatca aaccaatagt ctggcgtgtc
    17101 accccgacag cctctgccag ttgaccttgt gttaaatcac gctctacccg agctaatttt
    17161 aattttaaat ttttagccac cttcgtcctc cttatagttt taatactcat ctactcttta
    17221 aaaatccaaa ctaacacaag ctgtcagcta tttattataa ccccattaat ttgcactcca
    17281 gtattatctt cagccgaaaa tcagtatttt aggttgtttg aaataaactt cctagtttac
    17341 tcttttattt ttatttttat ttagcattag ctttagtagg atcttttaaa cgagttttta
    17401 ttgcttatat ttcttgttta gtttagttgg tttcatatca ctattatata atgcttttgg
    17461 aaatgagcct agtataagtc aaaaacgtgt tgaatagtga aagcaggagc ataccaatat
    17521 gactgatttt ttactgtatt ttaggtatgc aaaataatac taaatgaaac taaaatgaat
    17581 acttcttgct tttcttgaga gtgaaaaagt acaataaaat gattgtagtg attgccacaa
    17641 gatactgact aatagacatc gtatttgaag ccacaaatac aggtaaagaa taaaaaggaa
    17701 gaagttcaat tgctttgata aacaaatcat tactagtatt catcatcgta tttaatatat
    17761 tagggataaa gagtagtaaa gctactaaaa taccaagcca cttttcaatt tgtataaata
    17821 gaaaatgtaa gataatagaa aataaaacag gactaacaaa attgtattga ctacaatagc
    17881 tactataaaa tctaatccta aatcacctgg aatacccaat ttacttacta tgactagtaa
    17941 cacaatgcca ttcaaagcat aaataatgga agctgagatg gcatatagaa aattagacaa
    18001 gacgtaaatc atccgattct tctgttgatt ataaaacaaa cttaccgtat tgtgggaaaa
    18061 atctctacta atgactcgat tagcatgtat aacagccatt aacataccga gcatagcata
    18121 aaagtttgaa atatgcttaa taccaagcga tgtcccttaa gtctttagaa taaagaatgt
    18181 aagcaatatc ggcggtataa acgagataag caaactgaga tatattacgc ctgatgaata
    18241 taaatttctt atattttcct taagaaaatt caatttattt aatttcatga ttataacctt
    18301 cctttatttt gaacgattta ggtaaatatc tttaagcgtc tcttttttag tttcaaaatc
    18361 aactacttta atagagtttt cgttaaaaaa tttaaaaagc tcactacttt gaatattccc
    18421 agacatagta attctcaatc cctcttcctg aacaatatcc ccaaattctt gtttggtaat
    18481 gaaaatgtct ctatctgtag ctgatgaaaa agctatctca aacaagaaat tatgactatc
    18541 ttttcctact ttttgaaatg tcaaaagccc gttctccaag aaaagaactc tctcacaaat
    18601 ttcttcaatg tcttctaatt tatgactcga tattaaaatt cccacgtttt catttaaagc
    18661 taattttttt agaaccgcta aaactatttg tgatgattca atatctaaac cattagtcgg
    18721 ttcatctaaa atcaatatat caggttccgt aacgagagtt agaagcaaag ctaatttttg
    18781 ttttgtaccc aaagaatagg tctttacttt tttattaata gactgagtca aatctaactc
    18841 ttggatcaaa cttctaaatc tttcttgatt gtagtcaaca ccatataaat ttgacaaata
    18901 ttttaaattc tctaaacctg ttttagataa aaataatttt ggttcttcga ttaaataccc
    18961 aacattatca ctgcttataa tattacctga agtcggttga ttattttgaa caagaatttt
    19021 catcaacgta ctcttaccaa ctccatttct ccctactaga ccaacaattt cacccttgtt
    19081 aagtgcaaaa tttatatctt tgagaatgat tgaatgacca taatgcttac ttacattttt
    19141 attcctacat taatattcat ggtttactct ttgattctta ttgagtttaa aaagttgcgc
    19201 ctctttttaa caatattata tcatatatct aacttgatgc aatatataaa tgtcattttg
    19261 taaaatttta ttaacaaaaa actctctaca acaaaataat agagagtttt ctgcttatta
    19321 atttttttca aaatatctaa gacatcgctt ctttgtttaa tttgataggc tttgatacct
    19381 aagctccgac tttatcactt aaaaaataaa aaagacagct aatctcttgc gcatcaaggg
    19441 atttcgccat tctttaatct cgtttaagtg ttgtatatgg agctttgcat aattttttaa
    19501 aactaaacaa ctacattaag cacgaactgt gacgctggtt ccatcttcct tataaagatt
    19561 gataagcccc tctttaagag ctcgaaccat gtctcctgat tgaacgggtt gtgggac
//
LOCUS       DAVBHS010000024        19556 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVBHS010000024 DAVBHS010000000
VERSION     DAVBHS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19556
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.024"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..777)
                     /locus_tag="VQ651_000604"
     CDS             complement(<1..777)
                     /locus_tag="VQ651_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2753633.1"
                     /translation="MSKETVVYTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYAFTDYYLLTPDERKFVGLENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMVY"
     gene            complement(873..2183)
                     /locus_tag="VQ651_000605"
     CDS             complement(873..2183)
                     /locus_tag="VQ651_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET2753634.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGSGGGYEDKVNAAITTNSLPDVITL
                     DGPNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYN
                     KKMFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPAIDFRINSNDEMLPYAYM
                     PLIWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYP
                     MLLSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASE
                     FVKFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVA
                     YPQVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            complement(2310..3305)
                     /locus_tag="VQ651_000606"
     CDS             complement(2310..3305)
                     /locus_tag="VQ651_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2753635.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICN
                     ADSDSQNEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSENVCYVTSA
                     NYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKE
                     PLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKT
                     SVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIVIPTYFGGDKTTKKI"
     gene            complement(3667..5148)
                     /locus_tag="VQ651_000607"
     CDS             complement(3667..5148)
                     /locus_tag="VQ651_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2753636.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSS
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            5591..5755
                     /locus_tag="VQ651_000608"
     CDS             5591..5755
                     /locus_tag="VQ651_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET2753637.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            5839..6405
                     /gene="amaP"
                     /locus_tag="VQ651_000609"
     CDS             5839..6405
                     /gene="amaP"
                     /locus_tag="VQ651_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HET2753638.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            6417..6587
                     /locus_tag="VQ651_000610"
     CDS             6417..6587
                     /locus_tag="VQ651_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HET2753639.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            6626..7234
                     /locus_tag="VQ651_000611"
     CDS             6626..7234
                     /locus_tag="VQ651_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2753640.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            7265..7468
                     /locus_tag="VQ651_000612"
     CDS             7265..7468
                     /locus_tag="VQ651_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET2753641.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(7578..7856)
                     /locus_tag="VQ651_000613"
                     /pseudo
     CDS             complement(7578..7856)
                     /locus_tag="VQ651_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8054..8341
                     /locus_tag="VQ651_000614"
     CDS             8054..8341
                     /locus_tag="VQ651_000614"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2753642.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCSRQRRLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(8619..9389)
                     /locus_tag="VQ651_000615"
     CDS             complement(8619..9389)
                     /locus_tag="VQ651_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET2753643.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(9404..10252)
                     /locus_tag="VQ651_000616"
     CDS             complement(9404..10252)
                     /locus_tag="VQ651_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET2753644.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDRFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(10249..11724)
                     /locus_tag="VQ651_000617"
     CDS             complement(10249..11724)
                     /locus_tag="VQ651_000617"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET2753645.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYVSDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTSLKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(12047..13402)
                     /locus_tag="VQ651_000618"
     CDS             complement(12047..13402)
                     /locus_tag="VQ651_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2753646.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(13412..13729)
                     /locus_tag="VQ651_000619"
     CDS             complement(13412..13729)
                     /locus_tag="VQ651_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2753647.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(13746..14063)
                     /locus_tag="VQ651_000620"
     CDS             complement(13746..14063)
                     /locus_tag="VQ651_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2753648.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(14076..15761)
                     /locus_tag="VQ651_000621"
     CDS             complement(14076..15761)
                     /locus_tag="VQ651_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HET2753649.1"
                     /translation="MKNKKLISSLFILFLLIFIHVKVFANENLIKNSNFEQGELFWNS
                     RNRGIINSNESYSNGHYSGMIPSTAINDNRADGYIGQVIDIEPNEDYLVSAFAKVDIS
                     GAEGYFTARWFDNNKQGEIVQNNIGEAVDQTVNTTEWKKYMFSFNSGKHDKVLIQLVK
                     WSEDDTTKKSNIFIDNVEMYQLSKGNSYKKIWRDDFDGEQLNKKYWGYELGSIRGWEQ
                     QHYVRSDENVFLRSGNLVLRATNRSKEDQYFNPRNNHRKVVYNSGSVRTHGKVEFLYG
                     KLEMRAKLPKGQGVFPAFWTLGSDFTLDGKINPVQGRGWPSTGEIDIMELVGERNSNG
                     SGNKTVYQTLHYGQSDNDDGKFAGRGTAFKLSSGNFNDSYHTFSIDWYKDYIVWMVDN
                     HIVRKVYYGSDNIAKKIFNRPQYVQLNLAMGGSWPGVVDNNLSGTEFVVDYVSYSRND
                     LQQHQAEEYYSQSPKINGAKDIVINRGDVPDLLKGITTNSGYELDYSIENEPMFNNFG
                     GNTSVDLLIRNKTEKEKISQMPPGVYNLHYTSRPKHLDYESKVDRKTVTLTIK"
     gene            complement(15766..16662)
                     /locus_tag="VQ651_000622"
     CDS             complement(15766..16662)
                     /locus_tag="VQ651_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HET2753650.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(16665..17927)
                     /locus_tag="VQ651_000623"
     CDS             complement(16665..17927)
                     /locus_tag="VQ651_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2753651.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFITGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQGALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPSWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            18207..19385
                     /locus_tag="VQ651_000624"
     CDS             18207..19385
                     /locus_tag="VQ651_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET2753652.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT         6662 a         3777 c         2826 g         6291 t
ORIGIN      
        1 gtacaccata gtaattgttg agtttaatgg gccaccttgc gtcataacca ttggttgtac
       61 aatcagctta aaagcagaaa ttaaagttgt taacaataca aacagggctg ttggttttag
      121 taggggcatc gtgatataac gaaactgagc ccattttgag aatccatcta attcagcagc
      181 ttcatagaca tcttgtggaa tattctgcat tcctcctaaa aataaaagca tctgataacc
      241 tgcaccctgc caagcagaaa caaaaacaat tgcatacata gcttgtttag gactagtcaa
      301 gaaaggttga gaagcaatac ctaccttatt taaaatagca tttaataagc cactatttgg
      361 atttaaaaga taaagccaaa gaatagaaat aactaccaag gacatgacaa ctggagcaaa
      421 aaaagccact ttaaaaaaca tatttccttt tcgcttttta ttgacaatca aagccattcc
      481 taaggccgct cccaattgaa ctggaataat ccaaacaaca aatttcaaag tattcaagaa
      541 gctctttaag aaaatcggat cttttgttaa tcgaataaaa ttttcaagtc caacaaattt
      601 tctttcatct ggtgtcaaca aataataatc tgtaaaagcg taataaatga ccattccaac
      661 tggaattact aaaaagatac ctaataaaat taaagctgga gctaaaaaac tataacctaa
      721 tatgttatcc tttatttttt cacgtctttt tgtatacaca acagtttctt tactcatatc
      781 catctccttt aatcatatat atcaaactag gctattaaca agaaagggga aggtagttcc
      841 aaacattttc cttccacctt cttttttaca gattatttga gtgactgatc aatagcagtt
      901 tgcatttctt ttgtacgagt atccaagact ttttgcacat ttggattttc ttcataatag
      961 ctaatatctt gcattgcttg ttgaaaagct cgagaaacct gaggatatgc tacgacaact
     1021 gggcgagcat gagctgtttt tgaattttgt tccatcaaga aacgcattgg ttcggatact
     1081 ttatctttga tattttcaat tgtagattta cgaattggaa ggaccgaatt ccccaagcta
     1141 ataatttcac ttgattctgt atttgtagca aatttaacaa attcagatgc ggcctctttt
     1201 ttatcagatt ttgtcgtcac agctaattgc caactacctg aaggggatac taattttttc
     1261 gtcttactag atactgggta aggaaggata ccaaaatcaa tatctttgta gtttgtattc
     1321 atgtcagcaa tcgtccaaga accactcaaa agcattggat actctcctgt ttcaaaacct
     1381 ttttcgactg gactaacagt tgtatagcct tcttttacaa gattttgaat gaattgaact
     1441 gcttctacac tctctttgga gttgaagtat ccttctgcct ttgttccatc ttcgtttacg
     1501 acagaaccat tatttgacca aatcaacggc atataagcat atggcaacat ttcatcgttt
     1561 gaattaatac gaaaatcaat tgctggtttg ttataatgat ctttcaattt tttagcaatc
     1621 gtattaaatt cgtcccatgt ccatggcttt tctagcgttg gaagctctga ttccgcaatt
     1681 ccagcctctt tgaacatttt tttattgtaa taaacaccaa cattcgattc agaaaatcca
     1741 aatgcataga atttcccatt ataagttccc tgttgtttaa tactgtccaa aacatcatcc
     1801 atattattat cttttagata atcatctagc ggagtaatta ctttagattt tgcataagct
     1861 gccgtatttg gtccatcaag ggtaataaca tctggaagac tattagtcgt aatagcagca
     1921 ttaaccttgt cttcatatcc tccaccacta ccactacgag gaatatactc agttacaacc
     1981 ttgtacttac tttttcctga ttgattaaag ttatcaacaa ctttttgcca agcttttcct
     2041 tcatttgttt cgtcagagaa ttgtacccag actttaatat catttgaaga actcgcttgt
     2101 tttgagttag ttcctccact acaacctgca agtatagcta acgatagccc cgcaacaaca
     2161 gataacattg tacctttacg catcattttt ctcctttttg aaacgtttca ttttttattt
     2221 attttttaaa caagcttcta gcctattttt gaaacgtttc atttgttgat tatattataa
     2281 cagcgctttc attaattgtc aagcgctttt taaatttttt ttgttgtttt atctccccca
     2341 aaatatgtgg gaattacgat tcgtttttct agaatctctt ctcgcttaat cattttcagt
     2401 atcattgcaa cagattcttt tgccatcata ccaatatctt gaactattgt agacactaga
     2461 taagattgat cgacagaagt ttttaaacca tcaaacccaa ttatctggta atcatcaggt
     2521 gctattttcc ccagttcttt cataacacta ataactctca gagccatcat atcatttatt
     2581 gtaaatatac catcaatttc tttatgagat tgcaaaaaaa ttcttatctg atcttcagga
     2641 tttattaaag gttctttcat ttcaaaatta tagacatcta ctcctaattc cttagctttt
     2701 tctctgaatc ctttacttct gagtatagtc tcattctcat acatattaat tgaaccaata
     2761 aaagcaaggt gcttattatc ccttctaacc agttcctctg ctgcagtttg tcctcctttg
     2821 taatttgctg aagtaacata gcatacattt tccgaaaaat gtctatctat acttacaaac
     2881 ggtaaatcag ataaaatata tttgtcaata tcagaatatg taataccaat aattccatcc
     2941 actttatttt gtttcaacat ttcgatatat tcgatttcat tttgtgaatc gctatcagcg
     3001 ttacaaataa ataatttata attatatttt aataactctt tctcaacatg ataggcaaac
     3061 tccgaaaaaa atggatgcca tatgctcgga ataatcaaag cgatagtttc tgttctattc
     3121 tttttcatac ctcgagcata attatcaggg atatagtcca atgtttttat agccctttga
     3181 actttcctta aagttacttc tttaatgcct ttttctttat taattacacg tgaaacagtt
     3241 ccaacactaa ctcctgcttc taaagcaaca tctttcatgg taattgattt tctttgttct
     3301 accatattat cacctccttt caatatatag tatcatgcaa atgcttttta agcaactatt
     3361 tctcaatcat ttttggccag atcatttatc ccatcatgaa taaaatcact ccaattagct
     3421 tttgaaaata cttcaatttt catgtgtaaa catctacata aaacaggaaa agccttggtt
     3481 ttatggcttt tttcgtatct tctacaaaaa aagcaagagt tttagagggc tataaatcta
     3541 gatgtacatt ttgcttaaat gattgaaggt cttttcttaa caaaaacacc ccaaaaatta
     3601 gattttttct gtctaacttt tgaggtacag ttcaaacgcg aaatagcgtt tttttgttat
     3661 ttttggttac tcatctaatc gaataaacat catggcattt aacaaatata tgagtgagac
     3721 cgtgtttata ttatttgaat agatgagtct cttattttca ataggaggaa taataaaatt
     3781 agaaataatg atatcataag atgaatcttc taaagattcc tttgataatt ctaattcagt
     3841 ccaaacttcc agttcaaaat tattgctaca gtaataagaa agtgtctctg caacgaattt
     3901 tgcatgatac tgatcaaaat tactcataac caaaaccttt agtttaggct gattttgtag
     3961 caaattaatc accaaatgtt tggtatgagt gatgaaggta taagatagat gatttaccat
     4021 cattgaacta gaacaaacct caagagtctc taaatagtga gaaagctctt tttttatatc
     4081 tgaaacaaat tttggaaaaa tattttgaaa gttcctgatt gtattccctt tttgatcaaa
     4141 taaaataaac tcagtaaaca actcttgacg atacagatgt gcggtattat gcagatgcca
     4201 aatcagatta tccttattct ccatttcaat ctgatacttg actgaaatct gatcaataaa
     4261 atcactcaat agatggtaag atttttcaac ataactatcc ttttttacgc atttcataaa
     4321 gagactttca tctatgaaaa acattttttg aaagtaagac acaaataatt ggcaaacaac
     4381 ttcttcatct aaagagatat tgtattctga ttcaaaactc tgagcaacac cttctattcc
     4441 ttctgcctgc attaaaaaat ccaaactttg gtcgttaaaa gaatctttat ctacttccat
     4501 aaaatgacca aactttattc tatataggtt cgtaactagg agcaacttta gcattctatg
     4561 cgttgacaaa ttcattggaa agcttgtttc cttataaacc aattctaaca attgagatag
     4621 tggctctgat gaaaaatttt caaatggcca ttctaggaaa taatattttt ctgaaaaata
     4681 ttgtgcaaaa aagtaacgaa tgtctctctc atttccaatg atttgaacag gggtcagact
     4741 gacttcaaat tgaaattgcc ttttaatcac tttattgatt tggctaataa tacgatagag
     4801 cgaagatgaa ctgatataaa attctttaca aatactctca gcttgacaac cttcattaaa
     4861 gaagatgaat tctaaaatcg aaaaatgagt tgaatgttta aagaaatgat ggtaaaccat
     4921 ttcaatatca ctatcatcgg tattaataat gcgtatacca ttagtagaag aatgaaaaat
     4981 caagtcagga aaagcagatt taacatggga tagatcatct ttgactgcac gttctgtaca
     5041 atttaataac tctgctagtt cagaacgatg aaaccaacgt ttatgttcaa ataataattc
     5101 taataattct aattgcctat gacttttttt agataataaa tctctcatga atatctttct
     5161 ctctttataa attatcggat taaacctctt gcaattatac cacaaagaat aggtatagca
     5221 tgatataacg acttttccta aaatctttta tttcgtataa taacactacg gagacaatat
     5281 ataaacaatt ttcttatttt accgtctatt gagggcgtga atacagaatc aaattcaagt
     5341 ctaaagatta tatttttaat tttaaaaatt atataatagc aacaattaaa gaatttgatt
     5401 ttttaaaatt atataataat aacaatcgaa ataattgact tttctatatt aaagttatat
     5461 aatagtatta atcaaagaaa ttgatttttt gatattaaaa taaaaaagga gggtaggcag
     5521 tgttgtgatc aattattgct ggaggtctta ttggtctctt ggcaggtaaa atcactaaaa
     5581 aagtagttct atgggaatca tcgcaaatgt attcgctggt ttagtcgggg catatgcagg
     5641 acaatctctt ttaggtagtt ggggtccatc aatcgctgga atggctttgc tcccatctat
     5701 tgtaggtgca gcgattgtga ttactgtagt gtcattcttt acaggtagaa agtaaacttt
     5761 tcgccagtaa agttagcaaa ctatttttaa atcaatgacg ggaaaaatag tttaaatgtt
     5821 aaatcgaaag gattgtatat gtcaaaagca aagaaaatat gtttcattat tttctgtatt
     5881 ttaatcttga caattttcct tcctgttttg atagattatc atcaagttag tgatctaggt
     5941 attcatctac ttagctggag acagaactcc gtagttgaat tctatcttgc tagatatgtc
     6001 ttttggggga cagtggttct atcaacttta gttttattat ccattttagt tgtgatgttt
     6061 tatcctaaac gttacttgga aatccaactt gaaactaaaa acgatacatt aaaattaaag
     6121 aattcggcaa tcgaaggttt tgttagaagt ttggtgagtg atcatagatt gatcaagaac
     6181 ccaactgttc atgtaaattt acgaaaaaat aaatgtttcg ttcatgtaga aggtaaaatt
     6241 cttccttcag acaacatcgc tgacagatgc caaataattc aaaatgaaat aactaatgga
     6301 ttgaagcagt tttttggtat tgagcgtcaa gtaaaacttg aagttgcagt aaaaaattac
     6361 caaccaaaac ctcaaaacaa aaagactgtt agtcgtgtga agtaaggaag taaaaaatgg
     6421 aatggcttaa acaatatcga tatccaatta tcgctggtct cataggcgta tttctggctt
     6481 gtttgattgt ctcctttggc ttcttcaaaa caatatttgt attgatttta ggagcactgg
     6541 gagttgcagc tggattatat atcgaaaaaa actatataga taaataaaaa aataaaaatt
     6601 actaatttaa ttaaaggagt ttcatatgtc aaacgaaaaa aacacaaata ctaacgtaga
     6661 aaagaaagat gctactgttg tagctcacga aatcaaaggg gaacttactt acgaagataa
     6721 agttatccaa aaaatcattg gtctttcact agaaaacgtt tcaggtcttt tgggaatcga
     6781 tggtggtttc ttctcaaatc ttaaagaaaa aatcgttaac agcgatgacg taacaagtgg
     6841 tgttaacgta gaagttggta aaacacaagt tgcagttgac ttaaacgtta ttgttgagta
     6901 ccaaaaaaat gttccagctt tatattcaga aatcagagaa atcgtatctt cagaagttgc
     6961 taaaatgact gacttggaaa ttgttgaaat caacgtaaac gttgtcgaca tcaaaactaa
     7021 agaacagcat gaagcagact cagtaagcct tcaagatcgc gtatctgacg ttgctgaatc
     7081 aacaggagaa ttcacttcag aacaattcga aaaagctaaa tctggtcttg gatctggttt
     7141 ctcaactgtt caagaaaaag ttagcgaagg tgtagaagct gttaaaggtg cagcaaatgg
     7201 tgtagtatct cacgaaaaca ctcgtgtaaa ctaagataaa ataaatataa caggagaaat
     7261 tatcatgtca gtagaagaaa aattaaatca agctaaaggt tctattaaag aaggtgttgg
     7321 gaaagccatc ggtgatgaaa aaatggaaaa agaaggagca gctgaaaaag ttgtttctaa
     7381 agtaaaagaa gttgccgaag acgctaaaga cgctgtagaa ggtgctgtag aaggtgttaa
     7441 aaacatgttg agtggcgatg ataaataagg ttaaaagtta ctttatcttt ttggtaacat
     7501 tagtcaaaag agtctgagtc aagatgattc tcagaaaaca aaaagctaga gattcccaat
     7561 tgcggaactc tagcttttta attttgcctc tttctcttat tatatttcag caggttgttg
     7621 gccatgagta tgaatcccat gtcaattctc acttgacgct tacctctcag atgacatctc
     7681 ttataaccca aacaaacctt tatctgccca aagacagatt tcatatcaat cttacgttta
     7741 gcgaaaattt gtctaccctt ggaagataaa agtgcctgat attctttagt ttttaaacac
     7801 tggtaacgtt cattcatata cagtcccttt tgaggggctg attcaggttc ataatcgcag
     7861 tcaacattga tttcaaggct gtttgctttc tatctcccct ttttcatgat atattttcgt
     7921 acaggttctt caatagtttc tatagtaaaa tgaaccaaaa atagtacacg ttgcggtata
     7981 atgttcttgt ggcatattca ttagattttc gtaaacagtt ctctcctatt tgtgagcgaa
     8041 caagtatagt caattgattg tcagtttgaa gaccaagagt cgtaaggcaa aggatatggc
     8101 agaatccatc caagggtggc tggcacaatt tctagtgaac ctctttaaat ctattacgtt
     8161 tgactgtgga aaagagtttt caaaatggaa agatatttcc aatcatcacg atagtgagag
     8221 cttttttgcg aatctaggat gttcaagaca gcggcgtctc aacgaacatt caaatagact
     8281 tttaaggtgt catgacttgc caaagcagac agatttcaac gaagtcagtc aagaatttta
     8341 ggctggacaa aaactcaatt tcaggagaaa atgaaataaa tattcccaca ataaaacgca
     8401 tagtatcaaa gtttttccaa cacctgatat tatgcatttt ttaattttaa agactttttc
     8461 ccagcctcaa gaaatcacat cccacctaaa tcctttggat accaaatacc tcatcaggta
     8521 ttttctgagt tggtaagaat gtctagctta atttgacaaa tggagggcgt taaatccagt
     8581 ttttacagga agtctattta aatattttca cactgacctt acaaaatatg aaataaatta
     8641 tataaagtca ttcccatgac aaagaataac atccctttaa aacagtatct tacccaattt
     8701 tcaggtaaga cttttgatac aaccgtcccc aatacccctc cgcatataga agcaagaaaa
     8761 ataactggaa caggtgctaa atgatactga tttaaacctg taaccacaac aattgtagcc
     8821 aaatgagaca gctgggaaaa gaaaataatt gcaagagaat acatcgttgc ttcttttatc
     8881 gaaatagaaa aaaacaccat caataaagat acattgagcg gaccaccacc aatccctaaa
     8941 aaactagaaa ttaagcccaa tagaagacca caaatcatat agaatcctat cttatcaaat
     9001 tgataagttt ttttaaaatc tgttaaaaca aatgatacaa gtagcatcac aaatagtaaa
     9061 atcatttgaa caatggatac tagccgtact gataactgag ataacaaaac ttgaaaaata
     9121 agatgaccta gaaatcctcc taaaacagaa gaaaaggata ccgtaaaaat caatctccat
     9181 tttatctctt tactttgtga aaatcttttg attgttgaaa taatcgccat tataaaaaca
     9241 gagaaactag aataaaaagc aatatcactg accgaatggt atccaaaact atccataagt
     9301 ggcttaatta taattccacc acccatgcct gatatagaac ccaatccact agctattata
     9361 atgattagaa aataaataat ctcaaccact ttttaagtcc atcctattct ttcaaacatc
     9421 taaaacctag atggctacta gtggaagtag agatacatcc atttctagca gctactcggt
     9481 atcgattaca ataagaacaa tgacataaaa aggaacctcc tctgatagca tactcctcat
     9541 cttgtatccc atattttggc aactcatagt catcttcatt aaaactagct aataaggtat
     9601 atctcggatt gcgacaccat tcccaaacat tcccaatcat ttgatataga ccaaaatcat
     9661 taggtaaaaa ttcatagaca ggagccgttc ctataaatct atctaaggca gtattttcat
     9721 aaggaaatct tccttgccaa gtattggcat gataatatcc accttctaaa agagtatctc
     9781 cccaaggata ttcagattgt cttcctcctc tagcagcata ttcccactgt gcttcagtag
     9841 gtaaagacat tccgctccaa ttacagaaag ccaatgcatc ttctaaagca acatgaacta
     9901 ctggatggtc ttctaaaccc actagattac tgttctcacc gtagggatgt ttccagcaag
     9961 cgttcggaac ttgtaaccac cacggagccc cagcaggatg aggatatccc tccctttctg
    10021 cctctggaac gaacaatata aaaacaaaag accactcttg tcgctcagct agtgttttat
    10081 agcctgtttc tttcacaaat tgagcaaatt cttgattcgt aactggagta tccgcaatgg
    10141 aaaaatcctt tacactaaca gcgacttgag gaccttcaaa atcatctaaa aagccctctt
    10201 cagaatttgt tcccatcata aatgtcccac ccttgatttt tttcattttc atgattgact
    10261 atcccccgtt ttcgttagag ttggcgttat tttagaaagt tcaacaggaa ctaactcttc
    10321 atccacaaca aaaccttctt ctctatatct aagaaagtct actaattttt tcttcatctg
    10381 acgaacaata ggttggtatt tttcgcttgg atacaaatca ttcatttcat gcggatcttt
    10441 tttcatatca aataactgat aatgatttaa aacaggaaac cagataaatt tccactggtc
    10501 tgtcaagata tattgactag aatcttttcc taaagcatgt tcaccatgaa attctgttcg
    10561 ccacccctcg tactgtccaa ataataaatt cttaacactc cgtccatcta gttcatctgt
    10621 agtagttcct cctgccaaat ccacgagtga aggaaaaata tcttgaattt tcactaattg
    10681 cttgattgtt cccctattac cagctatcaa tccagccgga tcgtaaataa acgaaggaat
    10741 gtggatactt ccctgataag gatatccctt tctaaacaga taatgttcac caagctgatc
    10801 tccatgatcc gaaacaaacc agataatcgt atctttatca tgacgaaatt cttttaggga
    10861 agtcaaaaag cgactaattt gatggtctat atgagtaatc aatccaaaat aagcagcaac
    10921 cattcttctt tgatcatctt cctttaattt ccctcttaaa gcataaatag acggaatttg
    10981 cttttctaat acttcccaat tcccgatatg caaatcgaga aattgaggca agcgttccat
    11041 atacatatca aaatagtatt ttgggggatt taatggagca tgtggttttt caaaagacat
    11101 tttgagaaaa aaaggaaccg ttggatctct tctttgtaaa aatgaaatgg actcactcac
    11161 aacccagttt gttggatgta acttctcatc tttatcccaa ggtctagctt cccaagaatt
    11221 acaatccatt ccgtcatcaa tcaaatcaac atcataacca actttaccct ttaaaaaggc
    11281 tagatagtca ctaacataat caaattgact tccataagcc ttatcatact ttctatcaac
    11341 atgtagatac ccatcgtgaa gtaagacatg atcaaaccca agtctctgtc tggaaggaaa
    11401 aacatgcatc ttaccaatac actctgtttg ataacccata tctttaaaga cttttggaag
    11461 agtattagta aaattccaag gcacctcatc ttgataacca acacgtccac ttttatcttg
    11521 gtctaagcca gtcaataaag ctgccctagc aggaacgcaa gaaggaactg gagaataggc
    11581 attttcaaaa ttataaccta cactagccat catatctaat gttggagtgc taactagctt
    11641 atccttacta ttcaaggata aggcgtctgc tctcatttga tctacaacaa tcaaaataat
    11701 atttggctgt tttgtctgaa ccataaaatc tcctttctaa tatggcaaaa gaggcacaag
    11761 aagatatcta cctttactgc acccctttct atatcaatct ctctatataa agcaataaca
    11821 ttcttgttat gttttataga acaatgaact aaaatatgac taaatcgatt aggaaattca
    11881 aatcattttc tagtactgtt ttagaagtta cagtgtacta ttccaacttc aacaaactat
    11941 aaaccttggt ctaataacaa ttttagtgga gataagaaat cctacaccta actcatctta
    12001 cacgtaatct atttctattt tatcacaaaa aacgcaagta agaccattaa ctcaattcag
    12061 ttttatctgc cattttcaca aatgggaaat aagtcaagac actaataatc aaacaaacaa
    12121 ctgataagat gatggcacgc caatcaaatg ctgtagagaa gaaaccatat aaaattggag
    12181 gcattaccca agtaacattt tgtgtaacag gtgaaacaag accccagctt gttgcccagt
    12241 aagctaccgt tgccatgaaa accgggctaa gtacaaatgg tataaatagc aaaggattca
    12301 agacaactgg taaaccataa ttgaccggct caccaatatt aaacagaact ggtgctagac
    12361 caagtttagc aacttttcga taatgactgt ttcttgaaaa aattaaaata gcaagtacca
    12421 atcctaatcc tccaaaccag acaaacgccc caaaagaccc acttgtccat atataaggaa
    12481 tcggttcacc tttttggaaa gcatccagat tcgctaacat agcaactcca aacagccctt
    12541 ccatgatggg agccaataca tttcctccat ggagaccaaa aaaccagaat aacttattta
    12601 aaaagatcat cagaataact gcaaagaaac tttgagacaa acctagtaat ggcgtttgta
    12661 acaccttgta aacccaatca atcaataagt cattgctaag taaatggaaa acataagtca
    12721 agatggctac tatatacatc gccataaatc ctggaatgat agaagtgaac ggcttagcaa
    12781 tcgcaggggg aactgaatct ggtaacttga ttacccagtt ctttttcatt actttacaga
    12841 aaataataga ggctaaaaat ccaatcatca tggctgtaaa gtagcctctg gcattaatat
    12901 ggtttcctgg aatcacattc ccaatagtta ccatcagatt tttaccatca aatgctagat
    12961 tatcaattcc ttgtaagatc tgatctaatt tcacatctcc tacatttgcc aaagggaaac
    13021 tctttgtaac tgtacttcca atcgaaatga caaacgaagc aagtgatacc aaaccagcag
    13081 aaactgtatc aaccttgtaa atcttagcga tattcactcc caagcaatag atgaacaaca
    13141 aagaaacaat tggtatactt cccttgaata ccaaattatt gatgtcaaca agccactgaa
    13201 aggttttcgt aatactttct aggtgaaatt gttgtggtaa atccactaga aaagcattta
    13261 ataacaaagc aatggaacct gtcataataa caggcatagt ccccacaaat gaatcacgta
    13321 aagcaactaa aaacttctga ttcccaactt tcattgcaat tggagaaagt ttttcttgca
    13381 ttttatccaa cattgtattc atactaaact cctaattttt ttcaaatcgt tcgtatactt
    13441 ctaccacttc tttcgccaag tcaacaaaag tcagactcgt cattaaatga tcttgcgcgt
    13501 gtaccatcaa gaggctaatt gatgtttctt ccccctgtgc ctctcttgtc aacatttctg
    13561 tttgagcctt atgcgcctgt aataaggcag cattcgcatc agctaatctt cgattggctt
    13621 tagagaaatc cccttttttt gctgcctgaa tagcctccat agcattactt ttggcttccc
    13681 caccatacat aattagcccc attacagatt ctaaattact ttcatccata acattctcct
    13741 caatcttagt ctaataaact ttcagctaaa tctaaaactt tagaaccatt catcattccg
    13801 taatctgtca tcggaataac cgccacagga atctgtctat cttttagttt ttcttgaaag
    13861 tcccctagta aatagcgaac ttgggggcct aaaagcaaca catttacttc ttttgttgct
    13921 acaatttctt ctgcttcagg agctggaact gcaaaaattt ctgcatccaa ccccttatct
    13981 tctgctgcct tttgcatctt tgtcactagc agacttgtac tcatacctgc tgcacacgct
    14041 aacataattg ttactttagc catagcttac tccttttatt ttattgtcaa tgttactgtt
    14101 tttcgatcaa ctttagactc ataatctaaa tgtttgggac gactagtata atgcaaatta
    14161 taaactcctg gaggcatttg tgaaatcttt tctttttcag ttttattgcg aattaataaa
    14221 tctaccgaag tattccctcc aaagttatta aacataggtt cattttcaat agaataatct
    14281 aactcatatc cactattagt tgtaattcct tttaataaat caggaacatc tcctctgttt
    14341 attactatat ccttagcgcc atttattttg ggagattgac tataatactc ttcagcttga
    14401 tgttgttgta aatcatttct agagtaagaa acataatcaa ctacaaattc agtccctgat
    14461 aaattattgt ccactacccc aggccaactt cctcccatcg ctaaatttaa ctgaacatac
    14521 tgaggtctat taaaaatctt tttcgctata ttatctgatc cataatatac ttttctaaca
    14581 atatgattgt caaccatcca tacaatataa tctttatacc aatcaattga aaaagtatga
    14641 tacgaatcat taaagtttcc actcgataat ttaaaggctg taccacgtcc tgcaaatttt
    14701 ccatcatcat tatccgactg tccataatga agcgtttgat aaactgtctt attcccactt
    14761 ccatttgaat ttcgttcacc aactaattcc attatatcta tctcacctgt tgacggccat
    14821 cctcgtccct gaacaggatt aatcttccca tcaagtgtaa aatccgatcc aagagtccag
    14881 aaagcaggaa aaactccttg acctttcggt aattttgcac gcatctctaa tttaccataa
    14941 agaaattcta ctttcccatg tgttctaaca cttcctgaat tatatacaac ctttctgtga
    15001 ttgtttcttg ggttaaaata ctgatcttcc ttagagcgat tcgtagctct aagaactaaa
    15061 tttcccgaac gtagaaaaac attctcatca cttcttacat aatgttgctg ttcccaacca
    15121 cgaattgatc ctaattcata tccccaatat tttttattta attgttcgcc atcaaaatca
    15181 tctctccaaa ttttcttata tgaatttcct ttagacagtt gatacatctc tacattatca
    15241 ataaaaatat tagatttttt tgtagtatcg tcttctgacc atttcactaa ttgaatcaaa
    15301 actttatcgt gtttaccgga attgaaagaa aacatgtatt ttttccattc ggttgtatta
    15361 actgtttgat caacagcttc tccaatgtta ttttgaacaa tctctccttg cttattatta
    15421 tcaaaccatc ttgcggtaaa atatccctct gctccactaa tatctacttt ggcaaaagca
    15481 gatactaaat aatcttcatt aggttctata tcaataactt gtccaatata tccatctgct
    15541 ctattatcat taattgcagt agaaggaatc atacctgagt aatgtccgtt actataactc
    15601 tcatttgaat taattattcc cctatttcta gagttccaaa acagctctcc ttgctcaaaa
    15661 tttgaatttt ttattaaatt ttcattagca aaaactttca catgaataaa tattaataaa
    15721 aataatataa ataaacttga aattaatttc ttattcttca ttttatcacc tatttactga
    15781 cctagccaag cgagatactg tatgtctcac tcctctaatt gccttgctca atgcataaat
    15841 attatcgact gttgccagtc ccccatatcc tgcgtcacct atatgttgaa tatcaactcc
    15901 acaaatttta tttctaagtg caatttcctt gatagtatct gtatcagatg tttcttggct
    15961 agtaccaata gcactcagta ctagcccacc cttactatga acgagatcaa cgacttcacg
    16021 caactcttgg tcatgaaagg ctggaacggt tccaactgct ggaacaagta tcacatccgc
    16081 acctgcttcc aacaattgct ctgcgacaga aagctctgcc acaggctcat tcactcctgc
    16141 cccgtgcatc ttacctgcaa taatcaaacc agaaaagttt tccttagcag tttgaacagc
    16201 cttaatgatt tctcgattgc taactcctgt tccaggattt ccagtcaaac agacaaagtc
    16261 aaaacctaac tcctctattc gattcaatgt ttcaacacta gcaacacgac ctgcaacaat
    16321 ttcctgtgtt tcctctagca tctttgcaga tgggtcaatc ggttccaaat ttacaccaat
    16381 tggacaagca acaagctggt gtaagcgatg aataacatca tcacctgaac tatccaaagc
    16441 ataaatttta ggctcaaaaa catcaacaca attcaataaa atcatatcag ctccaaaagc
    16501 tctagcaatt tcagaattag ttatatcccc aacaaatgtc tcacgagtaa ctacattttc
    16561 agatagaaca actctacctt cacttgctaa aatactttgt tttaattctt cagcattcat
    16621 ctgtaatatc tcagatggat ttgcactaat taaacgtttc attctcaatc ctccaataac
    16681 ttaaaccgtt tttcaatagc tattacaaat ggtaaataaa gtaagacatc caaaacaatc
    16741 aatacaattt gtaataatga accagaaata cttcctgtag caagtaaacc acttataaca
    16801 ggtggcatag tccaacttgg atctgtataa gttagcggaa ccaaacctga tgccattgct
    16861 ccccacgcta ctagtaagtt aaacattgga gctaagataa atggaactag taacaaaatg
    16921 ttcaaaacaa taggaacgcc aaacattgct ggttcattta tattaaataa tccaggtata
    16981 acagttatag gtgccaaagt ttttagttgt ttactagctt tcttcttatg tgctaaatat
    17041 cctaacgcaa gaactaaacc tatggttgca ccaccgccac ctatgaatac aaaattatcc
    17101 ataaatacgc tagtaaaaat atttggaaga gtttctccag tttgataagc tactttattt
    17161 gcatctagat tagctaacca taacggtttc ataactgcat taacaacatt tcctccgtgt
    17221 aatcctacaa accaaaaact actattaagg aaaattaaga taagaagacc aataacatta
    17281 tttcctaaaa gtccaagtgg acctcccaaa gctccttgag caatttcatg aagattaggt
    17341 aaacttaatt tatctaaagt tgcataaaca atcaaccaac caacaatagt cactgcacca
    17401 ggaataatgg cactaaagct tttagatact gctggtggaa caccatccgg catttttatc
    17461 tgtatattat gatttataaa ccattggtag atatagccat taatcaatcc taaaatcata
    17521 gcaacaaata aaccttttga tgccatgaaa gcggtaggca ttcctgctcc agcctctcct
    17581 gtaatgaaag gcgtaactgt aataaaagat gataatgaca caataccagc aggaatacca
    17641 tcaactttgt attgtctagc cataaagtaa gcgataccaa aactagctac aagaccaagt
    17701 aatccaaaac tactatcaac acccttccat aaatagtcag caacaccaat atcacctaga
    17761 tatttttccc atcctggaat aggaaaactt gcaataacca ttagtaatga acctatcaac
    17821 aataaaggta tagttagagt aataccgtct cgaatagaaa ccaacgcttt attattaccc
    17881 aatctagccg ctactggaag taacttttct tgtaataatt tttccataac cttttcctct
    17941 taaatatatt ttattatcaa aagttatcaa ttatctttat tttttgacgt caagttatta
    18001 tttgattatc ttttgttatt tatattttac aactataaga aagccttgtc aaggatttgt
    18061 gtaaatattt gattattttt tattatcaat atataataaa aagagaccag taagcaatga
    18121 aaagcttaaa aagattaaca tctacattta tccatagcaa aaaatcatag gattttaaga
    18181 atctgacaac taggaagaaa actaatatga ccttatctaa gaaacaatta caactaagag
    18241 ctaaaatact ggaaacagtt tacacattag gtcccatttc acgaattgaa atcgctacta
    18301 aaacaggaat aacacctgca acaactagca gtatcacaaa tgatctgatt aaagaaaata
    18361 ttctcttaga acttggtgaa gacgaacacg atacaagcgt agggagaaaa aaaatcctgc
    18421 tagacattca agctaaacga ttttactaca ttggttgtga actatctgaa aaacatttca
    18481 cttttgcttt aggagataat ctaggaaata tcttaaagga agagaaagaa attgttacta
    18541 aacagttaat ccaagaaaag ggaaatcaac tcattaacca gacactaaag caattcttaa
    18601 ataactgctc tgattatgaa atagaagcaa ttggaatcgc gctacctggt cgctatttag
    18661 atgattataa aataacaaca aacaatcctt tatggcaaca tatcgactta gaaatgattc
    18721 aatcccactt tgataagcct ttgtttttct ctaacaatgt taactgtatg gctataggta
    18781 aacgcctatt tagtcgccaa cagaatgacc ctaattttgc ttattttcat tttgctagag
    18841 gtatgcactg ttcttatatc tacgacggaa acatttatgg gaaaggaaat ctaatgattg
    18901 gagaaatagg gcatacagtt gtttcatctg agggagaaga atgtagctgc gggcgcaagg
    18961 gatgcttaca aacttttgct ggagaatctt ggttaatcaa aaaatcaaaa attctatatc
    19021 atcaatctcc ctattctcta cttcctagtt tagtcaaaaa tgccgatgat attgatatcc
    19081 aagttatttt aaccgcttat caattaggtg acactggaat aatcacactt atccatcaag
    19141 ccttactcta tcttagtcaa accattttaa atatcagtat gatgattgac tctcaaaaaa
    19201 tctacctaca tagtccttta ctaacaaatc aacacatcat ccaaaaacta tatagtgaaa
    19261 tgaactacaa acccaaactg ctctataatc gcctacctga agtcattatt gaaccttaca
    19321 atgattttac agccgcacac tcagctattg cactttgttt gtatcataca attctacatt
    19381 cttaattttc tattacattt aataaatagg ctaaaaagag ggagtatatc ataaaaagcg
    19441 aataaacctg ttttctgaaa atcagaattc tgttttgttc gctttcttta tatttggaat
    19501 tatactccta tctccccttt ttcataatgt atagtgaaat gaaataataa caggac
//
LOCUS       DAVBHS010000025        18682 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVBHS010000025 DAVBHS010000000
VERSION     DAVBHS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18682
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.025"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..338
                     /locus_tag="VQ651_000625"
     CDS             <1..338
                     /locus_tag="VQ651_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2753653.1"
                     /translation="CLSETADKNWHKSDRIFVTNTGKTVHSSILSKSLQRANERLKKP
                     IPKHLSPHIFIHITISILSENKIPLKTITDRVGHPDSEVTNSIYTHVTKNMKDEAINV
                     LDKVMKKIF"
     gene            complement(830..1240)
                     /locus_tag="VQ651_000626"
     CDS             complement(830..1240)
                     /locus_tag="VQ651_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753654.1"
                     /translation="MPKKKIERISVIHREKILWLKWYFMRDKEKPKYSVLECKMFDAA
                     KNKDMLAYKKYATIKQITDIRVQTSEDDILTAIKEVYVYNHMNVIGACQRILFVSQSP
                     AYNKLNKWFETYSDLYFSIIPLPNMGAYHELVDS"
     gene            complement(1258..1635)
                     /locus_tag="VQ651_000627"
     CDS             complement(1258..1635)
                     /locus_tag="VQ651_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753655.1"
                     /translation="MRKYLKEIKELQELKELLSSRNTPEVIIVEGNDDLGEFFQVDGE
                     LFSDIELLENLKKWREWEVQVIVDDWCNRGLNEYETGILYFPKHEDKMDYIRFNKGLE
                     PLYHALDEPYTTISKSEWLKLLD"
     gene            complement(1577..2005)
                     /locus_tag="VQ651_000628"
     CDS             complement(1577..2005)
                     /locus_tag="VQ651_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HET2753656.1"
                     /translation="MAGNENDGLTSKQIKFIDAMLTEPTIDKACQKAGVSRATGHKYL
                     KVAAVKKTLRLKQDEMMDKTTQMLYLASSNAVSVLNDIMMDAKINPFIRTQAAKTILE
                     QSYKTHEIFGVVRQIEELRLEIEEVSKGDQRVTRTQGIIK"
     gene            complement(2049..2654)
                     /locus_tag="VQ651_000629"
     CDS             complement(2049..2654)
                     /locus_tag="VQ651_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753657.1"
                     /translation="MTFTTIKDDIKAFGKKKHGYMTGYIAKQEELHKQLQNGMIGEKY
                     AKDQLEAYKLEGDTYSRDTYNKIHAEIEKQHDLELEALKEKELSVTADDVAELTLLAS
                     MKMTKDELLGYFEKYKNKPLAIKKLWSIAEQYPEIAINLELFNAEQALESLILFFKRQ
                     LSYCHYSLLINGDKIQAVTTEMVVNSDAPELDRRLDEYLNK"
     gene            complement(2744..2917)
                     /locus_tag="VQ651_000630"
     CDS             complement(2744..2917)
                     /locus_tag="VQ651_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753658.1"
                     /translation="MTENKDNKLLEMMEKGFVLYSKNGIIKYIEIPDHGSIKLKAQDG
                     QLVYKEVTTGEQC"
     gene            complement(3516..4412)
                     /locus_tag="VQ651_000631"
     CDS             complement(3516..4412)
                     /locus_tag="VQ651_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET2753659.1"
                     /translation="MKSLQSSLGNIETFKLDELCPKHEIQLTQIKGERHVVVGWNENG
                     EVIKEVRCIPPYCEQCQEEQKKQDEEDAIADNLNAKLYLQTYNVLMSNKSYVADEFKT
                     KTFDDFNAVTSEEKRFLEFAKGQVQKYLDGMRGNTLITGGTGIGKTLLSVAIAKGINE
                     GYKTKGEPKSVLFVSLTEMIKQIKEGWNYGKGATLTEFEATELMKSVDYLIIDDLGAK
                     NAVIKPKSDWEQDLLFDVLNNRENTIFNTNLDSDELKIVYNERNYSRILKGLEGNTFK
                     AFTIKDKRYSINQLKNKVKQLK"
     gene            complement(4424..5233)
                     /locus_tag="VQ651_000632"
     CDS             complement(4424..5233)
                     /locus_tag="VQ651_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HET2753660.1"
                     /translation="MAKTKVYFWLKIDKKFFDNIFIKRLKTVPGGYTMTVIYIRLMLE
                     SLESDCILYYEGYFNNLKEELALKLDVSEDDIDMTMAYFTKCGLIQIDEDKNAELPQA
                     KAMVMSETNWASYKREQRKKGKLEEVQPSLTFSNSCPTEIEKELEIEVDKEQSPTPST
                     INQDFVNLYKSFEAETGKALSPLQIQELQYMLEDFSPELIHEALKEAVSQGKANFAYI
                     KAILNRWKQDNLLTVELVRNSRAAREAKKQQTNQLEPTSYEDWIPTQEKPF"
     gene            complement(5308..5523)
                     /locus_tag="VQ651_000633"
     CDS             complement(5308..5523)
                     /locus_tag="VQ651_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753661.1"
                     /translation="MNELDISNTQAVFVTVILIGLLLYLNHRDRKKSAQLERENQQTI
                     ETPSEDLNPDYGRYIQLAGVDVWGRIE"
     gene            complement(5525..5725)
                     /locus_tag="VQ651_000634"
     CDS             complement(5525..5725)
                     /locus_tag="VQ651_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753662.1"
                     /translation="MTLDLDNMTQAEFDEAMAEIQLQSPNIFQLISDFVNKKVTSVEI
                     DDLLNMKRAEQVAYIKNYKARA"
     gene            complement(5722..5913)
                     /locus_tag="VQ651_000635"
                     /pseudo
     CDS             complement(5722..5913)
                     /locus_tag="VQ651_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841301.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5902..6192)
                     /locus_tag="VQ651_000636"
                     /pseudo
     CDS             complement(5902..6192)
                     /locus_tag="VQ651_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            complement(6362..6514)
                     /locus_tag="VQ651_000637"
     CDS             complement(6362..6514)
                     /locus_tag="VQ651_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753663.1"
                     /translation="MKIVTFKPTKQIDGGFYLPGIDILFVSDKADAKSTEDVILFISR
                     NGLNKS"
     gene            complement(6525..6791)
                     /locus_tag="VQ651_000638"
     CDS             complement(6525..6791)
                     /locus_tag="VQ651_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753664.1"
                     /translation="MEILSKEIQLQGLQLLKQTLETLVELEKQRSSKLDLISRKELMD
                     LLGISATTLDNWEDLALKRYQTPMDGAKKVFYRLSDVYLFLAIK"
     gene            complement(7002..7184)
                     /locus_tag="VQ651_000639"
     CDS             complement(7002..7184)
                     /locus_tag="VQ651_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2753665.1"
                     /translation="MLITLSLAEKVRIKRARLQLTKKAVSEQLGIKSQTLTKVENGNY
                     DASKRIYESVMTWLVE"
     gene            7246..7749
                     /locus_tag="VQ651_000640"
     CDS             7246..7749
                     /locus_tag="VQ651_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2753666.1"
                     /translation="MYKMNRIKQLRKENNLTQRELANETKIPYRTIQRWENGETDIKS
                     DAAQVLADYFGVSIPHLLGYSDLIADTDSDIKAMAYSHLLTFVDEKQIKEFEKEFIRA
                     NDNSDTFGDLGSGEKALEKYYQALCYLVPPFRDILARYSFLSLDEKKSVYNIIVGLSN
                     NLSKENK"
     gene            7823..8986
                     /locus_tag="VQ651_000641"
     CDS             7823..8986
                     /locus_tag="VQ651_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET2753667.1"
                     /translation="MNIIEKIKKNGQRVYYASVYLGVDQLTGKKARTTVTATTKKGVK
                     VKARDAINTFAANGYTVKDKPTITTYNELVKVWWDSYKNTVKPNTRQSMDGLVRVHLL
                     PVFGDYKLSKLTTPILQQQVNKWADKANKGEKGAFANYSLLHNMNKRILKYGVAIQVI
                     QYNPANDVIVPRKQQKEKAAVKYLDNKELKQFLDYLDTLNQSNYENLFDVVLYKTLLA
                     TGCRISEALALEWSDIDLESGVISINKTLNRYQEINSPKSSAGYRDIPIDKATLLLLK
                     QYKNRQQIQSWKLGRSETVVFSVFTEKYAYACNLRKRLNKHFDAAGVTNVSFHGFRHT
                     HTTMMLYAQASPKDVQYRLGHSNLMITENTYWHTNQENAKKAVSNYETAISNL"
     gene            complement(9140..10444)
                     /gene="eno"
                     /locus_tag="VQ651_000642"
     CDS             complement(9140..10444)
                     /gene="eno"
                     /locus_tag="VQ651_000642"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET2753668.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            10608..11054
                     /locus_tag="VQ651_000643"
     CDS             10608..11054
                     /locus_tag="VQ651_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HET2753669.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(11051..12166)
                     /locus_tag="VQ651_000644"
     CDS             complement(11051..12166)
                     /locus_tag="VQ651_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET2753670.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIDVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGRHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(12175..12504)
                     /locus_tag="VQ651_000645"
     CDS             complement(12175..12504)
                     /locus_tag="VQ651_000645"
                     /inference="COORDINATES: protein motif:HMM:NF019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET2753671.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGGNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(12458..12820)
                     /locus_tag="VQ651_000646"
     CDS             complement(12458..12820)
                     /locus_tag="VQ651_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HET2753672.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHC"
     gene            complement(12879..14312)
                     /gene="glgA"
                     /locus_tag="VQ651_000647"
     CDS             complement(12879..14312)
                     /gene="glgA"
                     /locus_tag="VQ651_000647"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET2753673.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(14309..15448)
                     /gene="glgD"
                     /locus_tag="VQ651_000648"
     CDS             complement(14309..15448)
                     /gene="glgD"
                     /locus_tag="VQ651_000648"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HET2753674.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTFLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVRTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(15438..16580)
                     /locus_tag="VQ651_000649"
     CDS             complement(15438..16580)
                     /locus_tag="VQ651_000649"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET2753675.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(16570..18498)
                     /gene="glgB"
                     /locus_tag="VQ651_000650"
     CDS             complement(16570..18498)
                     /gene="glgB"
                     /locus_tag="VQ651_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET2753676.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEYGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDIYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEVIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         5537 a         3920 c         3148 g         6077 t
ORIGIN      
        1 attgtctaag cgaaacagcc gataagaatt ggcacaaaag cgaccgtatt tttgttacca
       61 atacaggaaa aacagttcat agttctatct tgagcaagtc tctccagcga gcaaacgaac
      121 gccttaaaaa accaattccc aaacatctgt cccctcacat cttcatacac atcactatta
      181 gcatcttatc agaaaataaa attcctttaa aaacaatcac ggacagggtt ggtcatcccg
      241 actctgaagt cactaattcc atctacaccc acgtcacaaa gaacatgaaa gatgaagcaa
      301 tcaatgtact ggataaagtt atgaaaaaga ttttttaaaa agttttgtcc cttttttgcc
      361 ctctaaatac aaaaatagcc cttcggataa aatccgaggg gctagaaacg ttgttaaatc
      421 aacggccgaa cttttgaatt tcatggttcg ggataaaata gttcactgaa ctattttatt
      481 ttttaaggtt atcataatat caaatagttc aattaaatac gctaaattac taatatactt
      541 tttacctttt tcattctaaa atgtaaagta caaacaatta caatatacta gagggggagt
      601 aaaaaaggta ataaaattga cgagttcagc aggcaaggga aatagcactt ttcatggtgt
      661 tattttttta attcagcaat ggagtagccg cttaattgat agaaacaaaa agcacctagt
      721 ttcctagatg ctagcacaat agcccggatt cgaaccggaa tctcctcaat taaggtgcaa
      781 tccctatata ctactcattg tgctttggtt cattatatca tacaatcaac tagctatcta
      841 ccaactcatg atacgctccc atattcggta atggaataat actaaaatac aaatctgagt
      901 aagtttcaaa ccatttattc agcttgttat aggctggcga ctgactaaca aataatatcc
      961 gttgacaagc tccgataaca ttcatgtgat tatacacata gacctcttta atggccgtta
     1021 aaatatcgtc ttcacttgtc tgtaccctaa tatctgttat ctgcttaatc gtagcgtatt
     1081 ttttataagc tagcatatct ttatttttag cagcatcaaa cattttacac tcaagaacac
     1141 tatacttagg tttttcttta tctctcatga aataccactt gagccataaa attttttctc
     1201 tgtgaattac tgaaatacgt tcgatttttt tctttggcat gattacctcc taaattatta
     1261 atctaatagc tttaaccact cactttttga gattgttgta taaggttcat ctagcgcgtg
     1321 atataaaggt tctaaacctt tgttaaatcg gatatagtcc attttatcct catgctttgg
     1381 aaaatataag attcctgttt catattcatt tagaccacgg ttacaccaat catcaacgat
     1441 aacctgcact tcccattcac gccacttttt aaggttttct aaaagttcaa tatcactaaa
     1501 taactcacca tcaacttgga aaaattctcc cagatcatcg ttaccctcga cgatgataac
     1561 ttcaggcgta tttctactac ttaataattc cttgagttct tgtaactctt tgatctcctt
     1621 tagatacttc ctcaatttct aacctcaatt cttcaatttg tcttactact ccaaaaattt
     1681 catgagtttt atatgattgt tcaagtatag tttttgctgc ttgagttctt ataaaagggt
     1741 taattttggc atccatcata atatcattga gtacagaaac agcattggat gaggctagat
     1801 atagcatctg tgtagtttta tccatcatct catcttgttt tagtctcaat gtctttttaa
     1861 cagctgcaac ttttagatac ttatgccctg ttgcccttga cacccctgct ttttggcacg
     1921 ctttatctat cgttggctca gtaagcatgg catctatgaa ttttatttgt ttagatgtaa
     1981 ggccatcatt ttcatttcct gccatctatt acctcctcgt tatcaaaata aaggggttgc
     2041 tagcccctct atttgtttaa atactcatcc aaacgacggt caagctcagg ggcatctgaa
     2101 tttactacca tttcagttgt tacagcttgg attttatcgc cgttgataag caaactgtaa
     2161 tgacaatatg aaagttgccg cttaaagaat aagattagac tttctagcgc ctgctctgca
     2221 ttgaataatt ccaagttgat agcaatttct ggatattgtt cagcaattga ccaaagtttt
     2281 ttaatagcaa gaggtttgtt cttgtatttt tcaaaataac ctagcaattc atcctttgtc
     2341 atcttcatac tagctaacaa ggtcagctct gccacgtcat cagctgttac gctgagttct
     2401 ttttctttta atgcctctag ctctagatca tgctgttttt caatttctgc atgaatttta
     2461 ttataggtgt ccctagaata agtgtccccc tctaacttat aagcctctag ctggtcttta
     2521 gcgtattttt cgcctatcat accattttgt aactgtttat gtaattcctc ttgtttagca
     2581 atgtagccag tcatgtaccc atgctttttc ttgccaaaag ccttaatatc atccttgatt
     2641 gttgtaaatg tcatgttatc ctccttacga aaaaataaaa gaggtatgac aaaagagcgt
     2701 taaactcaat tatcatacct ctggtttttc cagtcagtat ttattaacat tgttctccag
     2761 tggtcacttc cttataaact aactgcccat cttgagcttt tagcttaatg ctaccatgat
     2821 ctggaatttc aatgtacttt attataccat tttttgagta taaaacaaag cctttctcca
     2881 tcatttctaa taatttatta tccttgtttt cagtcatgaa tttctccttt atttcaagtg
     2941 tcccaacggg tacggttgta agctgagaca ttttagggct atctttgagc gacaaaaatg
     3001 tcgaaaagtc ttcagttcct gtataaccgt tattagtttt gtttgctact catttttgag
     3061 tcacaaatct gttaagcaaa aatgagtaat agctcgtaag ataagtaaat ctaatacttg
     3121 tattcctaaa gcgttgatat aactgatatt ttcaactatc caacgctctt tactaccctt
     3181 tttagtgtgc tttagctcat ttttgagctt ttttgagctt ttttgagttc cattttggga
     3241 atgcaaagca ggcgcgcacc gtgcatgctt ttttcttgct catagcctca ccctcagaag
     3301 tttgccgacc gagagcgtgg ggctttttat ttttgtataa ttaaaaacag ctgcacaatt
     3361 tttgtacagc tacgaccgag gcaggggcga cctgccgtac tttacactag ataatatcat
     3421 ctaggtagtc caactgtatt ttatcatatt aaaggctaca ttacaatttc tcaccctgtg
     3481 aagagtgtct atttttgttt aggtttgtaa gtcgattact tcaactgctt taccttattt
     3541 tttaactgat taattgagta tcgcttatct ttgattgtga aagccttaaa agtgttaccc
     3601 tcaagacctt ttagaatacg gctataattc cgctcgttgt atactatctt gagttcatcg
     3661 ctatcaagat ttgtattaaa tatagtattt tctcgattat tcagtacatc aaaaagtaaa
     3721 tcctgctccc aatcgctctt aggtttaata acagcatttt ttgcacctaa atcatcaata
     3781 attaagtaat caacagactt catgagttca gtagcttcaa actctgtaag tgttgcacct
     3841 ttaccataat tccacccctc tttaatttgt ttgatcattt cggttaggct tacaaaaagc
     3901 acactcttag gttctccttt tgtcttatac ccctcattta tacctttggc aatagcaact
     3961 gataaaagtg tttttccaat ccctgtacct cctgtgataa gcgtatttcc cctcatgcca
     4021 tcaagatatt tttgtacctg accttttgca aattctaaaa atcgcttttc ttctgatgtt
     4081 acagcattaa aatcatcaaa agttttagtt ttaaactcat ctgctacata gctcttattg
     4141 ctcatcaaca cattataagt ttgcagatat agtttagcat tcaaattatc agcaatcgca
     4201 tcttcttcat cttgcttttt ctgttcttct tggcattgtt cacaataggg tgggatacag
     4261 cgaacttctt ttattacctc tccgttctca ttccacccca ctactacatg tctttctcct
     4321 ttgatttgtg ttagctgtat ttcatgctta ggacacaatt cgtctagttt aaatgtctca
     4381 atatttccta aactagattg taatgatttc attttctgac ctcctaaaat ggtttttctt
     4441 gtgttggtat ccaatcttca tagctggtag gctctagttg attggtttgc tgttttttag
     4501 cctcacgcgc tgccctgcta tttctaacaa gttccaccgt caataaattg tcctgtttcc
     4561 aacggttaag gattgccttg atgtatgcaa agtttgcttt accctgactg acagcctctt
     4621 ttaacgcctc atggataagc tctgggctaa aatcttctag catatactgc aattcttgaa
     4681 tctgtaacgg tgacaatgct ttacctgtct cagcttcaaa agatttatag agatttacaa
     4741 aatcttgatt gatggtagag ggagtgggtg attgttcttt atctacttct atctctaatt
     4801 ctttctctat ctctgttgga catgagttgg aaaatgtcaa agatggttgg acttcttcca
     4861 attttccttt ttttcgttgt tcacgtttat aactcgccca gtttgtttca ctcataacca
     4921 tagcttttgc ctgtggtagt tctgcgtttt tatcttcgtc aatctgtatt aaaccgcatt
     4981 ttgtaaagta tgccatggtc atatctatat catcttctga tacatctaat tttagggcta
     5041 actcttcctt gagattgtta aagtagccct catagtataa aatacagtca ctttccaagc
     5101 tctctaacat gagcctaata taaataactg tcatagtgta cccaccaggt accgtcttta
     5161 gtctcttgat aaaaatatta tcaaaaaatt tcttatcaat ttttagccaa aaatagactt
     5221 ttgtttttgc catcgtcctc tcccttgctc atttataatt ctctaccttg attagtctta
     5281 atactcgctt gtcattcatt gttagactca ttcaattctt ccccatacgt ctacccctgc
     5341 aagctggata tatcgcccat aatcagggtt taaatcctcg ctaggtgttt ctatcgtctg
     5401 ttggttttct cgctccagtt gggcgctttt tttgcggtct cggtggttta aataaagcag
     5461 taagccaatc aaaatcacgg taacaaaaac cgcctgtgta ttgcttatat ctagttcatt
     5521 catgttatgc cctcgctttg taattcttga tgtatgccac ttgttcagct cgcttcatat
     5581 tcaacaagtc gtctatttcc acactggtta cttttttatt tacaaaatca gaaatcagtt
     5641 gaaaaatgtt tggactttgt agctggattt cagccattgc ctcatcaaat tctgcttgtg
     5701 tcatgttgtc taaatctagt gtcattgcat tgcctcctca aacttctcta taagacaact
     5761 tttatttgct ttctgagttc catttttaga gttaaaaaga atatatttta aggttacagt
     5821 agcctctaaa tactcctttt cagcatgctc tatatacgcc tgttgctctg cttcgttctc
     5881 aaaaaagtgc ttagcttagc gtttaaagaa tgctttgact aggtttagtt tgaattgccg
     5941 tactgcttcg gtattcttta gataagtgat cagcaaagta gcctgttgct cgttcagaat
     6001 ataggatttt ttaggttgtc ctctagtgtc taatttatgg attttaaatc caagtattcc
     6061 caactcttca aagtcaacct tattttctct tattaagcgt gtgatagtgt ggtgttgtac
     6121 gtcagcacat tcagcgatga tctcgcttgt ggtatacggc tctttcttgc cgtccatgta
     6181 gactagttcc attatggttc tacctcctgt ataaatttag ctagcttact ttttaattgt
     6241 ctcctctagc cctttttagc ctctacaacg gttttggcta acgtgttaga ggcttttata
     6301 acggcctgta tagctgtctc tatttcctcc gtggaatagt agctttgttt tctaatatca
     6361 tttaagactt gtttaaccca tttcttgaaa taaataaaat tacatcttct gtactcttgg
     6421 catctgcttt atctgagaca aatagaatgt caatgcctgg tagataaaac ccaccgtcta
     6481 tttgtttagt tggtttgaaa gtaactattt tcatttcata actcctattt tattgctaaa
     6541 aataaataca catctgacag acgatagaat actttcttag ctccatccat cggggtctga
     6601 tatcgtttaa gagcaagatc ctcccagtta tcaagggttg tagcacttat acctagcaga
     6661 tccattaatt ctttacgaga aattaaatct aacttactag atcgttgttt ttctagctca
     6721 actaaagttt caagagtctg tttaagaagc tgtaaaccct gtaactgtat ttctttagac
     6781 agaatttcca tttatatcct ccattttcct ttttataaat taagttgtct ttttcctcta
     6841 attttgataa aataagaaga gattatttag caaagggggt tgtccctttg ctaaatacct
     6901 aacaaaacca cgcgccttgc ctttgcttga gttttgttat ttttccttag ccgtgtacgt
     6961 tggtttggtc gcctgtacat ggctttttac ttgcgttaaa tttattctac tagccaagtc
     7021 atcacgctct catagatacg cttagaggca tcatagtttc cattctcaac ttttgttagt
     7081 gtttgagatt taatacccaa ctgctccgat actgcttttt tggttagctg taatcttgca
     7141 cgcttgatac gtactttttc agcaagtgat agtgtaatca acattggttt cacctccctt
     7201 ttttctccat ttttggcatt ttgtgttata atctctttag aaaggatgta taagatgaat
     7261 agaattaagc aattacgaaa agaaaataat cttactcaaa gagaacttgc taatgagact
     7321 aaaatccctt atagaaccat acaacgttgg gagaatgggg aaactgatat aaaatccgat
     7381 gctgctcagg tacttgctga ctattttgga gtatcaattc ctcacttact aggttatagc
     7441 gatttgatag cagatacaga tagtgatatt aaagcaatgg catactcaca tctactaact
     7501 tttgtagatg aaaaacaaat caaagaattt gaaaaagagt tcattcgtgc aaacgataat
     7561 agtgatactt ttggtgattt aggaagtggt gaaaaggcgt tagaaaaata ctatcaagca
     7621 ttatgctatt tagttcctcc ttttagggat atattggcta ggtatagctt tttgtcacta
     7681 gatgaaaaaa aatcagtcta taatattatt gttggtttaa gtaacaactt atcaaaagaa
     7741 aataaataac tttaaacatc atgtataaat acctaacaaa accacgcgcc ttgcctgctg
     7801 atggaaagaa aggtacaaat acatgaatat catagagaaa attaaaaaga atggccaaag
     7861 agtttattat gctagtgttt atctaggcgt tgaccaacta acgggcaaaa aagcccgtac
     7921 aactgttaca gcaaccacta aaaagggcgt taaagtaaaa gcgcgtgatg cgatcaatac
     7981 ttttgctgct aatggctata cagttaaaga taagccgaca attacaacat acaatgagct
     8041 tgtaaaagtt tggtgggata gttacaagaa tacagttaag ccaaatactc gccaatccat
     8101 ggatggattg gttagagtgc atttattgcc tgtatttggc gattacaagc tatctaaact
     8161 tactacgcct attcttcaac agcaagtaaa caaatgggct gacaaggcaa ataaaggcga
     8221 aaaaggggca tttgctaact actccttgct ccataacatg aataagcgta ttttgaaata
     8281 tggcgtagct atccaggtaa tacaatacaa cccagctaat gatgtcatcg ttccacgcaa
     8341 acagcagaaa gaaaaggctg ctgtcaaata cttagacaac aaagaattaa aacagtttct
     8401 tgattattta gacactctga atcaatcaaa ttatgagaac ttatttgatg ttgtcctgta
     8461 taagacttta ttggccactg gttgccgtat tagtgaggct ctagctcttg aatggtctga
     8521 tattgaccta gaaagcggtg ttatcagcat caataagaca ctaaaccgct atcaggaaat
     8581 aaattcacct aaatcaagcg ctggttatcg tgatatacca atagacaaag ccacattact
     8641 tttactgaaa caatacaaaa accgtcaaca aattcagtct tggaaattag gccgatctga
     8701 aacagttgta ttctctgtat ttacggagaa atatgcttat gcttgtaact tacgcaaacg
     8761 cctaaataag cattttgatg ctgctggagt aactaacgta tcatttcatg gtttccgcca
     8821 tacacatact actatgatgc tctatgctca ggctagcccg aaagatgttc agtatagatt
     8881 aggccactct aatttaatga tcactgaaaa tacttactgg catactaacc aagagaatgc
     8941 aaaaaaagcc gtctcaaatt atgaaacagc tatcagcaat ttataaaaaa taagggtgac
     9001 ccatttccgg gctaccctct tactatacca aaaattagta ggggtagtaa aaagggtatt
     9061 aaattataaa aagcactaag ggaaagcgcc ccaaagtgct tatttcaaag gctttatagc
     9121 ctataatcac ataaggagat tattttttaa ggttgtagaa tgatttcaat ccacgatatt
     9181 cagctacttc accaagttgg tcttcgatac gaagcaattg gttgtattta gcgatgcggt
     9241 ctgtacgtga aagtgaacca gtcttgattt gtcctgcgtt agttgcaact gcaatatcag
     9301 cgattgttga atcttcagtt tcacctgaac ggtgtgatac aacagcagtg taaccagctt
     9361 ctttagccat ttcgatagct tcaaaagttt cagtaagagt accgatttgg ttaactttga
     9421 taaggattga gttagcagca ccttcttgga taccacgtgc aaggtagtca gtgtttgtta
     9481 cgaagaagtc gtcaccaaca agttgtactt tcttaccaag acgttcagta agagctttcc
     9541 aaccatccca gtcgttttca tccataccat cttcaatagt gatgattggg tatttgttaa
     9601 ccaattcttc aaggtagtcg atttgttctg cagatgtacg aacagcagca ccttcacctt
     9661 caaatttagt gtagtcgtaa actttacgtt ctttatcgta gaattctgat gaagcacagt
     9721 caaatccgat aaatacgtct ttacctggta catatccagc agcttcaatc gcagcaagga
     9781 tagtttcaac accatcttca gttccttcga aacgaggagc gaatccacct tcgtcaccta
     9841 cggcagtttc caaaccacgt gatttaagga ttttcttaag agcgtggaag atttcagcac
     9901 cgtaacgaag ggcttcttta aatgttggcg caccaactgg caagatcatg aactcttgga
     9961 aagcgattgg agcgtcagag tgagaaccac cgttgatgat gttcatcatt ggagttggaa
    10021 gaactttagt gttgaatcca ccaaggtagc tgtaaagtgg gatttcaagg tagtcagcag
    10081 cagcacgagc tacagcgata gacacaccga ggattgcatt cgcacccaat ttacctttgt
    10141 taggagtacc gtcaagtgcg atcatagcac ggtcaatagc ttgttgatca cgtacatcgt
    10201 agccaatgat agcttcagca atgatgttgt ttacgttgtc aacagctttt tgtgtaccaa
    10261 gaccaccgta acgagatttg tcaccgtcgc gaagttcaac tgcttcgtgt tcaccagtag
    10321 aagctcctga tggaaccata ccacgtccga aagcacctga ttcagtgtaa acttctactt
    10381 caagtgttgg gttaccgcgt gagtctagga cttcgcgagc gtaaacatca gtaataattg
    10441 acatttttta ctctccttat gagttaaatt ttttacacct ctataatacc ttaaaacccc
    10501 tcctttttca agaaaaaacg ttatctttgt gcaacttttc cttaacttta taaagtaatc
    10561 gctttctttt gtctgtttta ttctaacttt tatgatatac tgttttcatg acagatttat
    10621 caaaacaatt acttgaaaaa gctcatggtg ggttaaaaat aaatccggat gagcaaagac
    10681 gctatcttgg tacttttgag gaaagagttc ttggatatgt agatattgac acagcaaata
    10741 gccctcagtt agaaaaaggc tttttattta ttttagaaaa ccttcaggaa aaagcagagc
    10801 cactatttgt gaagatttca ccaactatcg aatttgataa gcaagttttc tacttaaaag
    10861 aagcaaaaga aactgatagt caagccacca tagtatctga agagcatatt acttctcctt
    10921 ttggcctggt tattcatagc aatgcaccag ttcaagtaga agaaaaagac cttcgacttg
    10981 cttttccaaa actttgggaa gttaaaaagg aagaaccagc caaaacatcc ttatggaaga
    11041 aatggtttag ctaaatcttg cacatattta ataagtgccc aatattggca gccgtgcgct
    11101 ccagatagag actggcattt ttcaaactat cttctaaagg ttcacttttc tccaaaatag
    11161 aaaagacagc ttggatattt tcaaatggta ggggaggtaa atcttcagca agactaccgc
    11221 aaatagcaat aacaggaact ccaacagggg ttctttttgc aacacctata ggcgctttcc
    11281 cagcaaagct ttgactatca agtcttcctt ctccaacaac aaccaagtca gcatctgaaa
    11341 ctttcttatc aaagttgatt aagtccaagc aggtatcaat tccagacacg atacttgcct
    11401 gagcaaaagc acacaaacca ccagcaaggc ctccacctgc tcctgctcct ttaatttcta
    11461 atgttgcagg tgagaatttt tcataaaaat cttggatcgc ctgatctacg actgcaaaca
    11521 tagtcggatg tcgacctttt tgattgccaa aagtataagt cgcaccttga tgaccacata
    11581 agggacttac gacatctgct aaaatatgaa tttgaacacc ttcaggaatt ttatagcaat
    11641 tttctgttga aacagaagct aagtttaata aggattgacc ggaagcaggc aagacatttc
    11701 catccctatc ataaaattga taacctaaac cagcagcaat ccccagtcct ccatcattac
    11761 tggccgtgcc accaacatcg atataaatat ctttaatccc tttagaaatg agatggagaa
    11821 tcaactctcc aataccacaa gtttggattt gaagtggatt tcgtttctct agcggaattt
    11881 ttccaagacc aaccaagtca gctacttcaa atagtgccag ttcccctttt tgaaaatagc
    11941 gcatggcttc tttttgtcca aaagggtctg tcacttggat ccatttttct tttaggtcaa
    12001 gagaatgtcg gatagcatct acagtacctt ctcccccatc accaacaggg cagaggagac
    12061 attctacatc tgctatcgat tgttggaagc ctctttttat tgcttcagct acctgttgag
    12121 ctgtcaagct ttccttaaac gaatccggtg caattacaat cttcatattt tccctcattc
    12181 taaacagtca atcaaaggaa gaacttctaa aaaatccctc ttgtcaacat gatgtggtat
    12241 ttcttttttg agcatttctt tggaacaaaa ggcgattcct aactccgccg acttcaacat
    12301 taatagatta ttgcccccat caccgattgc caccgttctt tctttagaaa gttttagttt
    12361 ctttctccat ttttccagag tttctttttt gacctgggga cttataattt gtccaactaa
    12421 ttttcctgtt aaaagacctt ctttgacttc aagctggtta gcagtgaaat aggcaatacc
    12481 aagggatttt gctaatctct ccaactattg gtgtaaatcc accagacacc agaccaacta
    12541 ggataccatt cttttggaga atagagatga actctgggac atttagcgat agatgaattg
    12601 agttgaagac gttatcaaag accaaaatag gaagaccttc caacaaggac actctttttc
    12661 ttaaactgct ttcaaagacc aactctcctc gcattgctcg acttgtaatc tgcgaaattt
    12721 ctgcctcatg acctgcctct ctccctaaaa gatcaatcac ttcttctagg attaaggttc
    12781 catctacatc caaaacacac aagcctttta cttgagacat cagttctcct ctctaaacag
    12841 cctaaaaatc gtatgaagtc atcatacgat tttatctatt aattaactaa actatggtac
    12901 aagtcaaggt atgacttgca ggctgtatcc catgagaagt cactctccat agcttgtttt
    12961 tgtaggtttc tccaaatgtc tggatggttt ctatacaagt ccaatgctgt ttggaaagtc
    13021 caatttaacc aataaggaga tagattgtca aagctaaagc cagtaccgct tccttcgatt
    13081 ggattgaaag cgcgaactgt atctcgcaag cctccaactt catggaccaa tggcaaggtt
    13141 ccataacgca tagccatcat ttgagacaag ccacacggtt caaaacgact tggcatgagg
    13201 aagaggtcac aagcagcgta gatttcctga gcaagtttga catcaaaagt gatatttgtt
    13261 gatagcttgt ctgggtaaat ctgagcaaac catgagaaag ctccttcaaa ggctggatcg
    13321 ccagttccca aaagaacaat ctgaacatct tcttgcaaga tatggtgaag actttcgacc
    13381 accacatcaa aacctttttg acgtgtcaaa cgagaaacaa ttcccaccag tggaacgtct
    13441 gctctaacag gcaagccaac tctttcttgc aattttgcct tatttttggc tttcccagac
    13501 aaatcttcct gattgaaatg atagtctaaa agagcatccg tctgaggatt ataaagatca
    13561 gcatcaatcc cattcacgat accagatact ttaccagact ccattttaag aatctgatcc
    13621 aaattacatc caaactgact agtcataatt tcatgagcat agctaggtga aacggttgaa
    13681 acacggttcg catagagaat acctgccttc atccagttca gacagttgtt ccatcgaagg
    13741 gtgccatcag cgtaacgttc aaagccaact ccaaacaaat cacccaacat tccttctgaa
    13801 aattgtcctt ggaattctaa attatgaatg gttaaaactg tttcaatgtc ctcataggct
    13861 tgaatccaac ggtatttttc cttcaacaag aaaggaatca tagctgtatg gtagtcatga
    13921 acatggagaa gatcaggaat aaagtcaatc ctttccatag cctcaatggc agccagttgg
    13981 aaaaaggcaa agcgttctcc gtcatcaaaa tcaccgtaaa catgaccacg gaagaaataa
    14041 tattgattgt caataaagta gaaggttaca ccatttaata ctgttttctt aattccacaa
    14101 tactgtctgc gccaaccaac gctcacctca aaatgaagca catcttcaat ctgatttcca
    14161 aatttagcct ctaccatatc atagtagggt aaaatcactg caacttcgtg cccagctttt
    14221 accagtgatt ttggaagagc gccaatgacg tctcccaaac cacctgtttt tgaaaagggt
    14281 gcaccctctg ctgctacaaa taaaattttc atgaatgaat atcctctgtt actttagcac
    14341 ctttcttaac cacaactgga tgttctgcag ttcctcgaat cacaacacca ggctcaactt
    14401 caaccccttt gtccaagata gcatattcga cctgagcccc ttctccaata acaacacgag
    14461 ggaagagcag gctatcttta accaagctat ccttatggac atggatatta cgtgatagaa
    14521 cagaattagc tacttgacct tcaataatac taccagaggc aaactgagaa gttcttacct
    14581 tagatgtatt agcatagtaa gttggctctt cgtttttgac ctttgtataa atcttttggt
    14641 ttggtgagaa aagagaatag aatttttgtg attcaagcat atcgatattc gcttgataat
    14701 aagatttaac agagtgaata ttggctagat agcccgtgta ctcgtaggcg aaagctccct
    14761 cttttacagc caaatcccgt aaaacatagc gcaatttctc tggatgttct tttttagctt
    14821 cttcttccaa gtgttcaatc aaccaaggtg tatcaacgac aaagatatct gtagacatat
    14881 tgaacgtttc agctgttgac ttgctatcaa agagtttatg agaaagaaca tggtctgttt
    14941 catctacatc caagattgca tttacttctg aaatatcttt cttagctagt tttttataaa
    15001 ctacagtgat aggctctttt gttgtactat gtaggtggaa aacttggttc aaatcaatgt
    15061 taataagaac atcgcagttg agggcaaccg tttggtttga gccagaacgt ttcaaataag
    15121 taagaagctg ttggtagtat tcttttccaa ctgtactact ttctacacgg gtattgtaaa
    15181 ttcctagata gtaatggcta agaaaggttg ataagcccca ctcgcgtcct gaacgaatat
    15241 ggtcaaatac tgagctgata ttatcctgct ggaaaatacc aaagacacta cgaacacctg
    15301 cattagcaag gcttgaaagt gggaagtcaa tcaaacgata tttcccacca aatggcaaac
    15361 ttgctactgg acggtggtcc gtcaatgtcg acatattgtg aaaaccaact gtatttccta
    15421 aaatggcaga atatttatca atcttcatct gttgctaccc ccactacttc attatatcct
    15481 acaacttgta cttcatctgt tccatcaatt tcgacaccgt cagaaataat cgcaccttca
    15541 ccaataatgg cacgtttaat cttagctcct tgaccaatga tagctccact catgataact
    15601 gaatcaagga cttccgctcc ttcgcgaact tgcgcgcctg ttgaaaggat agaatgttta
    15661 acagttccat caacgaaaca tccgtctaca actaatgagt cttccacatg agcatttgcc
    15721 ccgaggaagt ttggtggtga aatcaagttt cttgagtaaa tcttccattg acggttacga
    15781 ctatccaagg cattttctgg agaaatatac tccatgttcg cttcccaaag tgactcaata
    15841 gtaccaacat ctttccaata accactaaat tcgtaagcat aaacactttc acctgactca
    15901 aggtaatttg gaatgacatt tttaccaaag tctgacatgc caaccttgct cttttcagca
    15961 gcgactaaca tattacgaag gcgttgccaa tcaaaaatgt agattcccat agaagctttt
    16021 gtagatttag gttgagctgg tttttcttca aattcaacaa tacgattgtt agcatctgtg
    16081 ttcatgatac caaaacggct tgcttcttta agagggacgt ctaaaactgc tactgtcaag
    16141 ctggcattat tatccttatg agactggagc atatcatcat agtccatttt gtagatgtga
    16201 tccccagaca aaatcaagac atactcagga ttgacactgt cgatatagtc gatattttgg
    16261 taaatagcgt gactagtccc ctcaaaccaa cgatttcctt cacttgcaga ataaggttga
    16321 agaatagaga cacctgaatt aataccgtct agtccccagc ttgaaccatt cccaatatgg
    16381 ttgttgagag caagtggttg atactgtgta acgaccccaa cattatgaat ccctgagttg
    16441 gcacagtttg atagggcaaa gtcaatgata cggtagcgcc caccaaattg cacagctggt
    16501 tttgcgatgc tttgagtgag tttaccgaga cgagttcctt gcccaccagc aagaatcaaa
    16561 gctaacattt catttttcat tttctactcc tttttggttt ttatttgtga cggttttagt
    16621 agatttcaag cgacgtttga ttttccatac acttgctccc atagccggta gggtaaaggt
    16681 taaggtctgc tcataatctt tccatagtcc ttcttgcgtt tgaacagttt gattatgttc
    16741 tttccaaacg cctccccact cttccaactc agtattccat acttcttcgt aaattcctgc
    16801 aacgggtagt ccgattgtaa aatctttccg ctcaacaggt accatgttaa agatacagac
    16861 taacatttct ccctttttac ccttacgaat aaaggaaaga acactctggt ctcgattatc
    16921 cgcatcaatg acttcaatac catcatagct ggtatcgatt tcccacagac agcgatgatc
    16981 tttgtaaaac tggtttagct gagaagtgaa atacttcatc ttagcattca ttgggtcttc
    17041 taggttagac cattccaact gttcttcaga tttccattct aggaattgac cgtattcgct
    17101 acccatgaag agcaatttct taccagggtg acaaatttgg tacgtataga gattgcgcaa
    17161 gcctgcgaat tgattgtaac gatctcccca catcttatgc atcatactct tcttgccatg
    17221 aaccacttca tcgtgcgaga atggcaagag ataattctcc ttgaaaacat acataaagct
    17281 gaaagtcacc aggttaaagt catatttacg ataaatcgga tcttcttcgt agaaacggag
    17341 gatatcattc atccagccca tgttccattt gtagtcaaat cctagaccac caatctcttt
    17401 cattcccgta atcttggttg cagacgaact ttcttctgca atcatcatca catctggata
    17461 ttctaactta ataacttcat tcaagcgttg aaggaaataa taaccttcat agttgagatt
    17521 tccgccatct ttattaggtg tccatggagc atcatcatag tccaaataga gcatgttgct
    17581 aacagcatcc acacgaatac catccaaatg atagatatca atccaatgct taatgcaaga
    17641 aattaagaag gactggactt cattttttcc aaggtcaaaa ttaagggcac cccaaccatg
    17701 gttatgagcc ttattatggt cttggtattc aaaagtcggt gtcccatcat aataggctaa
    17761 ggcatcatca ttgatggtaa agtgacctgg tacccagtcc acaataaccc caatattatg
    17821 ggtatgacac tcctcgacaa aatcttgaaa ctcctctggt ctaccatagg catgctctaa
    17881 agcgaagtaa cccataagct ggtaccccca actcaaacct agtgggtggg acatcaaggg
    17941 cataaactca atatgagtat agttcatctc aactaggtaa ggaatgagtt catctttcaa
    18001 ttgggcaaaa ctataaggac tgccatcagg atttctcttc caagagccag catgaacctc
    18061 gtaaatattg acaggacgtt cagcaaagcc ccaacgtttt cttcgtgcca gccaaagtcc
    18121 atccctccat ttcttatcag gaagctctgt tacgattgcc cctgttcctg gacgagcctc
    18181 atacctgaca gcaaaagggt caatcttcat cagttgatga ccattttgac gtgtgacatg
    18241 atatttgtaa atatgccctt cttgagccat attggtaaag acttcccaga ccccaaagtc
    18301 atttcttacc attggaatct gattttcaat ccaattggta aaatcaccaa ccaagtgaac
    18361 agcctgagca ttaggtgccc aaacacggaa ggtatagcca tactctccat ttagttcttc
    18421 cctatgtgct cctagataat gttggagata aaaattttca cccgtcataa aggtttttaa
    18481 tgcttctcta ttatccatat actccctttc tcctgtaagc gttttctatg tttttattat
    18541 actacctttt tagagaagat tcaagtaaat tactatactt ctttaattat tttgaaaatc
    18601 tacaacaagt tcacttactc gttcaattgt aaatcaatat tttttcaaaa aattgcgaaa
    18661 acacctttct ttttctacta ta
//
LOCUS       DAVBHS010000026        18116 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVBHS010000026 DAVBHS010000000
VERSION     DAVBHS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18116
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.026"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..1879)
                     /locus_tag="VQ651_000651"
     CDS             complement(<1..1879)
                     /locus_tag="VQ651_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET2753677.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(1873..2742)
                     /locus_tag="VQ651_000652"
     CDS             complement(1873..2742)
                     /locus_tag="VQ651_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2753678.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPDLENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNILCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQDVKKAVDNFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(2826..5471)
                     /locus_tag="VQ651_000653"
     CDS             complement(2826..5471)
                     /locus_tag="VQ651_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HET2753679.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDERFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELIVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAFRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVFRYYNMSQENVHISEHQQTILDLLE
                     RPYPVHSGPLDPQEIRTEFIKKEEI"
     gene            complement(5562..6842)
                     /locus_tag="VQ651_000654"
     CDS             complement(5562..6842)
                     /locus_tag="VQ651_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HET2753680.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSIDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            7013..9097
                     /locus_tag="VQ651_000655"
     CDS             7013..9097
                     /locus_tag="VQ651_000655"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HET2753681.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGKAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKENKWLDIHTEQNHSHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPNH"
     gene            complement(9137..10816)
                     /locus_tag="VQ651_000656"
     CDS             complement(9137..10816)
                     /locus_tag="VQ651_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET2753682.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNLDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFDCRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIYVLAFQAGETEKRLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            11571..12800
                     /gene="arcA"
                     /locus_tag="VQ651_000657"
     CDS             11571..12800
                     /gene="arcA"
                     /locus_tag="VQ651_000657"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HET2753683.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELILSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGNLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            12858..13874
                     /gene="argF"
                     /locus_tag="VQ651_000658"
     CDS             12858..13874
                     /gene="argF"
                     /locus_tag="VQ651_000658"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET2753684.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            14039..14986
                     /gene="arcC"
                     /locus_tag="VQ651_000659"
     CDS             14039..14986
                     /gene="arcC"
                     /locus_tag="VQ651_000659"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET2753685.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVIVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            15195..16706
                     /locus_tag="VQ651_000660"
     CDS             15195..16706
                     /locus_tag="VQ651_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HET2753686.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNTWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            16728..18059
                     /locus_tag="VQ651_000661"
     CDS             16728..18059
                     /locus_tag="VQ651_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET2753687.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAEDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
BASE COUNT         5058 a         4134 c         3767 g         5157 t
ORIGIN      
        1 aagtcgtata aatcgtcgaa gctgtcgcaa tggtatgcca ctggttagct gtagttgctg
       61 cgaaatcctt gtctgcgtag aagtcagtaa atggtaggat ttcaacttcc agctcgtcga
      121 tgcggtcaag ctggccagcc agataaacct cgatacggct ttctgctcgt ttgatgcgtt
      181 gcaagagtcc gcccatacgg atgtcaactg tatccaaacc aaagaccttg ttttctttca
      241 gccattggtg actaaagagg gcatggaagt cttcaatttg gcttctaagt tctggtaatt
      301 cttgtctggc gatttgttgt aaactttctt tatcatccgc ttggtaggcc tgacgaatgc
      361 gtcgtcccac atctactttg ctacttaaaa tagcattcaa ctgggcctga gtttcaaaga
      421 gataagcata gtttccagct ttttctttaa tgttagcaag cgtctcagca gcctgagcga
      481 agtgcggttt gtcctgttca ggtgtcatgt gttgatcaag aatcggacaa agaatatcct
      541 gataaaaaac atagcggttg ggattgatac cgctgagatt gcctggtagg tctggtaaga
      601 ggttggcaag gtcaatctgc ataaaatcct caaccgttag accagtattg gtcttgaagt
      661 gcgcagacaa accatctagg tcattgcgat agctgagttc tgcccagatt tgcaagcttg
      721 gtaggataga gaactgggca gtttcaccac cattgtctcc ccaacccgtt acgatgactt
      781 ctttaatctg attggcacgg caggctttat tagcctcgat agccactaga cggctaaaat
      841 ggttgtgagg tgtaaagcca atccacttcc aagctccccc tgcaaatgca aggtcatggc
      901 taatcttgtg atgattgcgg aaattacggt tgtatttttc ctcgctatcc tgataataat
      961 cccagtaaac cagagtcaca cggtctttga gacggtctag gtagacacga gtttcctctg
     1021 gaatttccac atcacggtcg tactggccat ccgctgacat gagtttgaag aacatatcac
     1081 tccacatctg gcagtggaaa ccatatttgt cagcaatatc cagcacgcgc tccaagtgtt
     1141 ggcacatgag gagactacga tccacaacac cgttcaaaat caggtagcgt cccaaaccaa
     1201 ccaagtgggc ttcgtccatc ccgatattga ccttgcgagt cttcagttta gacaacgtgg
     1261 caaacatgcc atcaatcagg tcataaacct tttcttcgcc aatgagaaga atgtcctcta
     1321 catcacggag ctcctgcact tccttgacac cccatttgac aaaggccgac aagtgggcca
     1381 aggtctggat gcatggtaca aaggtcatgt caaactgttg agcataggct tcgatttcct
     1441 gcaactcctc tgctgaataa gctccacgga aatagccaaa gtaaggctgc ccttcaatct
     1501 ggtaagtgtc ttccatgtaa agttcaaagg ttgagtagcc catgagagcc aagacctcaa
     1561 tcatctgctt ggcagaagcc acattcagca ccgcatttcg agaacagtca accatgtaag
     1621 ccaaatcttc gtaagctgct tgttcctcaa tctctacttt atcagcttct gctagaactg
     1681 ttaccaacaa ggataaggca cgataaagtt ggtgaggttt gcggtaggtc aattgatagt
     1741 gaccttcctc accctggata gagatagagg cttggtcaga ctgagtgaca gccacttcca
     1801 catctggtag agaaatatga ccttttaaaa cctcaatagc ttgcgtttgt ttgggactaa
     1861 gtcctgtaaa tcttaccatt gcttttcctc ctgtagccag ttgacaagag caccgtagag
     1921 attggcatct gcgtgatagg tgcaggcctg gataactggt gcgaccgtgt attcttcgta
     1981 ggtatcgaca aagttatcaa cagccttctt gacatcttgg ataaaatctg gattttgact
     2041 gatagagcca cccagactga tgacatctgg atcgatcaga tactggatat tgagcaagcc
     2101 ttgcgccaga ttgcggttca tgcgctcaat ggcttcttga caaaggatat taccagctgc
     2161 ggcctcttgg taaatcttgc gaccgtccca atcagtatga ccagattttt caatcacgta
     2221 tcgtaccata ttcccagttg atgctagttg cgaccagtta ttaagttttt cagcaggggc
     2281 aagggttgtc atgtagccaa attctccacc caagccgtgg cgacctcgat gaagtctacc
     2341 attgataatc atggctccgc caatccctgt cccaatcacg acacaggctg cattttcaag
     2401 atctggatga gctagtagtt cactgagtcc aacgcagttg gcatcatttt ctaaatggac
     2461 aggtagctga taagagctaa gcgcctcata ccaagaaaag ccatggatgt agggcaccgc
     2521 actgaagcca tcaatcacac ctgtctcttg attgactgca cctggaacgc tcatagcaat
     2581 cccactgtaa tcctgttctg acaagcgttg atctagccac gctagtaaat cctccaagtt
     2641 ttcaggcgtt gaaatacttg tcttatccag tattttccca tcaggagtca gactggcaaa
     2701 cttaatccca gtccctccga tatcaatcgt tgcaatggtc attgttttta ccataaaaag
     2761 gggcgaatca gcagttagtt cttacttttt cgcccctcct ttcttttgat gtttactttt
     2821 tgaaattaaa tttcttcttt tttgatgaat tctgtacgaa tctcttgtgg atccaatggt
     2881 cctgaatgaa ctggatatgg tcgctcaagt aagtcaagaa tggtttgttg atgttctgat
     2941 atgtgcacat tttcttgact catattatag taacggaaga catagccttc ttcattttcc
     3001 gccactttaa aggctgttgg acaaacttgc ggtatgctga gaacagaatg gctcaagagg
     3061 ctaccagtcg caaccacgct tccttcctgt ctagcaagct gaaggctggt aaacggtgtc
     3121 tgcaaggctt tggcacgacg gaaagctgaa aagcgttctt gggcttggtg gcattcaagt
     3181 gcaaactcga cttcaaactc acgcaagcat tgtgcttctg gcgttgggaa gtagccccag
     3241 tcacctagct cacctgatgc acgcaaaatg gtcacggcaa tggtgttatc cccaaggatt
     3301 tcgtattcat tcaatccctt attggataca gtcacccctt tttcatcgtc atacagactg
     3361 acaaaagctt gttggtgttg aggattttca ggattttccc atgaagcagc tggtttgttt
     3421 ggtcgtgtca ccacctcata gatactttca gaatcattgc ttggacgcgt gttatgagtc
     3481 ttgaccaaga gacggatacg gtgatccttg gcagtgttag taaagcgagt cttgaagcgg
     3541 atttgtggat tgtcaacaaa gacaatcaac tcagtttcca gaggaatgtt tgtcaattct
     3601 tctgaccgtc cagcctcacg cttcataaac tcgatgatac cttgttgctc ttcttctagc
     3661 ttttcatccg cactgacagg cacggtcaat tcatgtttga gcaagatttt agcatagcga
     3721 tctgtgtttt ccaagacctc gtggccctta agctctgcaa agattggctc tgttcctttt
     3781 ggttggaaat agatatactc gtttccgatg tccccacggt cttcgaagcg gataaagtct
     3841 tcataggctt cgtgagttgt cttgtcatat actgtgatat tgtcatccac actcaccgtt
     3901 acgaatggtg tatcaatcac tccgttttgg taaataccct cacggtgttc ttgttttcct
     3961 tccagcaatt ggaaggttgt ccaagaaagc ggcgctaggt gaactggaat ggtcacgcgc
     4021 acttgtcgag caatacgagc ttggcggaac ttgtcttttg gtaaatcgta ctcaaaatta
     4081 gctccgaggt cttcgattgt agcctctaca ggacgaccat ccaagtcctc cacacggtaa
     4141 cttggtaaga taagagcagc catctttttg tagccttctg ttgggtgcaa ttccttgaaa
     4201 tcacaagtcg ccacatcaat cactgtgctg acagtatcga ccttatcatg caagcctgtg
     4261 ttaatgacag taaagagata gtcactttga gccttatccg tagcaatttt acccttccac
     4321 tcgttgagca agttactttt aacaaagttt cctacttggt tgaccttggc aaaacgcgtt
     4381 tccatctcgc ggtgaacttc gtccacgcta cagccacaga tactatcatg tggcgcattc
     4441 tgcaaaagtg ttttccaagc ataggtcaac tggtccttgt ggttgtgtcc accagtgata
     4501 atagtcaagg gttctaccac ttgctctagg aggttgctat tttcttggaa ggcttgtttt
     4561 aggtaaatgc gggatgaaga agtgttggca agtgtgtacc agccatctgt ttcctgactg
     4621 gtcaactcgc ctgtaacagt tgataagtgt tcaggaagcg caccttctac agcttgaaca
     4681 tattcatcaa aagaactatg aacaaagatt acatccggga agagttcatt tgccacacga
     4741 atggcttcgc tcagattttt ctgtacaggc tggtggtcac agccgttcat catcaaccat
     4801 tggttggtcg aagcgtaggc acgcacatct gacaattttt gtttccagaa ggtcaaggcc
     4861 tcatctttgt caactgggat ttcattccca ttactgtacc agttggcaaa gagaatacct
     4921 aaaacacgac taccatccac accctgccag tacatttctg aaaactggga tgtaaaccgc
     4981 tcatcttcaa ggacttggtt gtcaaatcca atcggcttca caccacgacc aaaggctgcc
     5041 acgtgaatac ctgatttttg aaggatttga ggagcttgtc ccatatttcc aaaggtatct
     5101 ggaaagtagc caatctgggt tgatttaccc catttggcag cttcttgttg accaatcaag
     5161 gtattgcgga cattggcttc actggagatc aagtagtcat cctgcaagat gtaaaaggga
     5221 ccaattttaa gtttgccctc gtcaatgtag cgttggacct tgtcgcgatt ttcagggcga
     5281 atttgtaagt agtcatcaag gacaatagtt tgtccatcca agtggaaact cttgaactca
     5341 gggtcatttt caaagagatc aaagagattg tcaaacaatt ccaccaactg catacgatgg
     5401 ctttcaaaag gcaagtacca ctcacgatcc cagtgactat gtgagataat atgtacaaca
     5461 acattttcca tgaagtaaaa cctcattcta aatttaaatt ttaacgtttt aaatgtaaac
     5521 ttgtgattct gataagaatc ggttgagcta aagcgagccc cttagcggat atccaagtaa
     5581 tccaagacca actcacagaa catcatgtta gcccaggaga accattcacg agagtagagg
     5641 gtcggatcat ctacgtggaa gctttcgtgc atgacacctg taccaccatc gcaggcaacc
     5701 agctgatcca gcaagaattt tttctctgcc ttatctcttg ttgtcaatcc ttggatagaa
     5761 agggcgattg gccagatata gcgatagaag gtatgagaac ttccgagacc gctagcgtat
     5821 tctccttggt agaagtatgg attttcagag ctcagaatgg tacggcgagt ggcttgatac
     5881 acttcatcat cgatcgaaca gtagcccaga tagggcgcag ctagtagact tggtacattt
     5941 ggatcatcca tgatgctggc atttcctagg ccatctactt caaaagcgta aatcttttcg
     6001 cccttgctgt tggtggtgta agcgtagttt tcgattcctt cttggatttc atcctgaaga
     6061 cgcttggcat cagcaataac actctggcta tcagctaggt ttaatgctgc gaagatttct
     6121 tgcacataac ccaagactac tacagcaaac atatttgacg gaatcaagta actatactgg
     6181 caacagtcat cactcggacg aaaagctgac caagtcatac ctgtcactgc aaagtcaggt
     6241 ccaaagccat catttaccaa ggtgtcttcc ttacggtccg tatctcggac aaaacgataa
     6301 ggagagttct tgtggtcttg ttcaactgtc catagatgga gaatttcctt agtcgctgcg
     6361 acaaaagttt catcaaactg actagtctcg ccagtctctt tccagaggag ataagccaac
     6421 tgcaaaggat agcaaagcga atccacctca tacttgcgct cccagatcca gccgttaagg
     6481 tctgtgtggt cagtctcgtg gtgccctttc cagttctcct caatgttgaa ggagttagca
     6541 tagggatcct tgagtaccaa ggtcatctga cgtttgacca aacctgcaat ggtctgacgc
     6601 aggagggcat ctcttttagc tacatgaagg tagggtctga gttgggctgt cgaatctcga
     6661 agccacatgg caggaatatc cccagtcaag acaaaagttg aaccatcttc taagatttca
     6721 accgtattgt ccaaggtatc ggtgtagcaa cgctcaaaga catcaaccca ctctggatgg
     6781 tccttggccc gctctgctac ttcatctagc cattctctaa caatttcttt cgaataaatc
     6841 atcgtgcaat tccctctttc aaacaagttt catgttcagt atatagcttt tgagacagaa
     6901 aatacataca caaatgatag tcatttttct acaaaatagt tatctttaaa actccttttc
     6961 taaaggccct ctttttctga tataatgtag aaaaacagct aaaggaaaga ctatgaaacc
     7021 actacttgaa accatcgata cccgctttgg tactgctagc aagcatgcct tctctcgggg
     7081 aaataccctg ccatacacag gcgtgccttt tgggatgaat tactttgtgc cccagaccag
     7141 tgaccaggat ggatcttggt tttttgatcc gcatctgcct atctttcagg ggattcgatt
     7201 aactcaccag cccagtcctt ggattgggga ctactcttgg ctccttctga cacctgtcac
     7261 cagtcaactg gggggagaca gcctctttca ccgccagtct tcttatgata tagataaagc
     7321 ttgcttccag cctcactatt tgaagctctt ttctctgcgt tatcagattg aaacccagct
     7381 cacaccgact tgctatggtg cttctattcg tttgaatcaa aagcaaggca aagccctctc
     7441 cctctatctt cacgcagcag atgaactgac agtagagcaa gtagataagc ggactcttgc
     7501 cctgcgacaa gaaggtaaaa ctgaaactaa caaaaattcg ctaacgatgt tcactgccct
     7561 gcaaatgaac acggatattc ttgctatcag ccaagaagct ggggactggc gaattgactt
     7621 agcaagtagt caaaccgaga tgcaactagc cacttctttc atctctcctt ctcaagctct
     7681 aatcaatcta cctcaagaag attttgatag ctgtaaaaca agtgcccaag cggattggga
     7741 aaatctcctc catcgttttg acattataga gacaggagag gctgaccgaa ccttctttga
     7801 ccactgcctc tacagactct tcctcttccc acagactttt tatgaggttg atgagtcagg
     7861 gaaagccatc cacatggatc tggctactgg tactgtcaag cctggtgtcc tctttagcaa
     7921 caatggtttc tgggatacct tccgcaccac cttccccctc tttgccctta tcataccgga
     7981 gcactatcaa cgctttttag aaggtttcct caatagctac cgcgatactg gtttccttcc
     8041 aaaatggctg gctccagatg aacggggtat gatgccaggt acactgctag atggcattat
     8101 cgcagatagc gcctgtaagg acatggcccc cgacctagaa ggagaactct tccaagccat
     8161 gctcgaaaca gccagcaaag ccgaccctct cggcatcaat ggccgccacg gactagccca
     8221 ataccaagaa ctaggttacc tctctaccga ccaccacgaa agtgttagcc atactctaga
     8281 ctatgcctat agtgactttt gtatcgccag ctgtgccaaa aaactagaga acatagaaat
     8341 cgctgaaacc tacaaggctg cttcacaaaa ttaccgccag ctatttgacg ctgagacagg
     8401 ttacatgcga gcacgagaca atcaaggaaa ctttcaccct gacttctctc cttatagttg
     8461 ggggcgagac tacgctgaat gctctgccat tcaagctact ttaggcgttc tccacgacat
     8521 ccctggctta atccaactga tgggtggaaa agaaaccttt agcaactatc ttttgaaagc
     8581 ctgtcaagat gctcccctct ttgaaacaac aggctatggt tacgaaattc acgaaatgag
     8641 cgagatggct actgctcctt ttggacaaat agccatttcc aaccagccta gtttccacat
     8701 tccttatctc ttccgctaca gcgattaccc tgactacact gcccttctta tcaagacgct
     8761 ccgtcagaaa gcttttcacc caagttggga agcctatcct ggagatgaag acaatggtag
     8821 tctctctgct tggtacatct ggtcagctct cggattttat ccaacctgtc caggaaaacc
     8881 aagctatgat ctcggaatcc ctctctttga ccatctacga gtctacctag ctaaagaaaa
     8941 taaatggcta gatattcata ctgagcaaaa ccacagccat tttaactttg tcaaagaatg
     9001 ccgactggac aaaacactcg tatcaactat tcaacaccaa gacctcctaa aagctgaaca
     9061 actaactttc accctcagct ggctaccaaa tcactaataa ccaaaagaac ctttgcatcg
     9121 cgcaaaggtt cttcttttat gaaacttgga cttgaagttg atccacaagc tgaccatcca
     9181 ccgttaggtt cagatagaaa tccaaggttt tatctcctgc gaaatacaag gttggtagcg
     9241 tcagcctttt ttcagtctca ccagcttgaa acgcaaggac ataaatctcg tcctgatagg
     9301 cgacaccatg gacacctgtc cccggttgaa tcgaaatctt agcctctaaa gaggagctac
     9361 tttcactacg ttcaatccta aaatgaagag tctctccctt tgccacagct aggctttttt
     9421 ctgcaaatgc tagttcctga acaacttctt tttttgataa cccaggagtt ttataaaggg
     9481 aaatcttggt caatacaggg atagattgtg cctcggtaat catcacgcgc accttttgtg
     9541 cctctactag cggacctcgc aagaggcgct tatgaccaac tgtgaagccc ctgccaaact
     9601 cctgccagac accatccacc tctacttgca catgaaaagc agcgattcgt tgccctagct
     9661 tcaaatcttc tcttaactca attacatcaa aagttttagg agaacctaaa tcgagttcta
     9721 actggatggg caagtctgca tcgcttgccc aagagctggt ctcaaggccg tctgtcaaat
     9781 ggcgacaatc aaagtctgcg gaaagagctg gaccagatac ctcagctccc agagccaaat
     9841 cttctttata gagctcattg cgataggtcg caaattcata aagtcgttca atatcctttg
     9901 catcaaagag cccagcttga ttcggcggaa tattaagcaa gagtggagtt cctcgcccta
     9961 ctgagtgaaa gtagatttcg accaactcct cgagagactt aggatcctga tcctcatggt
    10021 agaaccagcc tggacggatg gaaacatctg cctctccgat tgaaaaaatc gtgcccgagg
    10081 gatccccgtg ctgaagatag ttcagctctg cttctgttcc tagtttatca agattcacct
    10141 tttgccacag tggatcacct gcataccctc gttcattgcc aatccagcgg atactggtgc
    10201 cttctgttga aaaaatcaag caatcgccct gcaggtcacg aatggtttca aaccattttt
    10261 caaattcata attaaccttt tgcgcgccct ctcctctggc accatccatc caaacctcag
    10321 cgaacttacc agcattccca tagttaggat ttgataagat ttccttcaac tgagccagat
    10381 aataggcatt gtagtccgct tctcggtcca catgatagag gggactatgg gcatcccacg
    10441 gtgacaggta gacccccata tccatatcaa actctgtggc agcttgggat acttcaagga
    10501 gcaagtcgcc ctttcctctc ctccaaggac tgaccttaac cgaataatct gtgtgagctg
    10561 tcggataaag gacaaagcca tcgtggtgct tgaccaccaa aatcaacttt ttgaagcccg
    10621 tttccttgag cacacgaacc cactcacgcg catccaactg actcgggtta aagcgctcag
    10681 gatcctcctg tccagtcccc cattcttggt cataaaaggt attaggaccg aagtggataa
    10741 aagctgctag ttcatctccc agataagcta gctgagtctg acttggtaac ggtccatgcg
    10801 gtttgatttt cttcatcgtt atatatctcc ttcttttgca acttctcttg ttcacaaggt
    10861 tatccacagg ttgtggataa taaaccatga tctaacagcc agctctttcc aactccaact
    10921 ggtaggctca ggatttttct cctcaatgtt ccctcttcaa acagctctgt catatggttc
    10981 tgctaacacc tctcagaatg ctactctcca taattattct caggcttctt tcaggtcatt
    11041 gtgtgaagac cattcactct ccagactttt ccacagattg tgaataagtg attattctaa
    11101 gctatcacct cgtcgactat aacaaaaaat aaaaagaggg tcaataagat aattatctct
    11161 tcctaaatac tcagaaaaga ttctataatt tgtacaattg aacaagaaat gcttctccaa
    11221 aatctatctt caaaagaatg ttgcatcttt ttatcctttc acacaaacat ttctctttat
    11281 acagatccaa gtgataacca gatgataatt ggacactttt tggtcatttg tatatttttt
    11341 gaaaaatttt ttaaaaaaat tttatatcct ttttatagaa gtttatgcat ttgtatctat
    11401 gtattctatc ctatgttatg tataataaca taaaattgtg ctaaacatca taatttatat
    11461 tattttccga gaaattatag ccattgacat tacctaggaa ttgcgttata acatacatgt
    11521 aagcggtacc caaaatggtg ccaagtcaaa atttttaagg aggaaaatac atgtcttctc
    11581 atccaattca ggtcttctca gaaattggga aactgaaaaa agttatgttg caccgtccag
    11641 gcaaggagtt agaaaacttg ttgccggact atcttgaaag gcttcttttt gatgatattc
    11701 ctttcttgga agatgctcaa aaagaacatg atgcatttgc ccaagctctt cgcgatgaag
    11761 gaattgaggt tctctaccta gaacaactcg ctgctgaatc attgacctct ccagaaatcc
    11821 gcgatcaatt tatcgaggaa tacttagacg aagccaacat ccgtgatcgt caaaccaagg
    11881 ttgctattcg tgaattactt cacggcatca aggacaacca agaattggtt gaaaaaacaa
    11941 tggctgggat tcaaaaagtt gaattgccag aaattcctga cgaagctaaa gatctaactg
    12001 acttagttga atcagattat ccatttgcaa ttgacccgat gccaaacctc tatttcactc
    12061 gcgacccatt tgcaacaatt ggaaacgccg tatcgcttaa ccacatgttt gcagatactc
    12121 gtaaccgtga aacactctac ggtaagtata tcttcaaata ccacccaatc tatggcggaa
    12181 aagtggattt ggtctacaac cgtgaagaag atacgcgtat cgaaggtgga gacgagctaa
    12241 ttctttctaa agacgtcctt gcagtaggta tctctcaacg tacagacgca gcttctatcg
    12301 aaaaactttt ggtcaacatc ttcaagaaaa atgttggctt caagaaagtt ttggcctttg
    12361 aatttgctaa caaccgtaaa ttcatgcact tggatactgt cttcactatg gtagactatg
    12421 acaagttcac tattcaccca gaaatcgaag gcaaccttca cgtttactca gttacttacg
    12481 aaaacgaaaa acttaaaatt gttgaagaga aaggtgactt agctgaactt cttgctcaaa
    12541 accttggtgt agaaaaagtt catttgattc gttgcggtgg tggcaatatc gtagcagctg
    12601 cgcgtgaaca atggaacgac ggttctaaca ctttgaccat cgcacctggt gtggtagttg
    12661 tttatgaccg caataccgtg accaataaga ttttggaaga atacgggctt cgcttgatta
    12721 agattcgcgg aagtgaattg gttcggggcc gtggtggacc tcgttgtatg tctatgccat
    12781 ttgaacgtga agaagtgtaa tcgctgttcg gtattcgtca atagaaaatg taaaaaatag
    12841 aaagaggaaa taataaaatg acaaattcag tattccaagg acgcagcttc ttagcagaaa
    12901 aagactttac ccgtgcagag ttagaatacc ttattggtct ttcagctcac ttgaaagatt
    12961 tgaaaaaacg caatattcaa caccactacc ttgctggcaa gaatatcgct ctcctatttg
    13021 aaaaaacatc tactcgtact cgtgcagcct ttacaactgc ggctatcgac cttggtgctc
    13081 acccagaata cctcggagca aatgatattc agttgggtaa aaaagaatct actgaagata
    13141 ctgctaaagt attgggacgt atgtttgacg ggattgaatt ccgcggattc agccaacgta
    13201 tggttgaaga attggcagaa ttctcaggcg ttccagtatg gaacggtcta actgacgaat
    13261 ggcacccaac tcaaatgctc gctgactact tgactgttca agaaaacttc ggtcgcttgg
    13321 aaggcttgac attggtatac tgtggtgatg gacgtaacaa cgttgccaac agcttgctcg
    13381 taacaggtgc tatccttggt gtcaatgttc acatcttctc accaaaagaa ctcttcccag
    13441 aaaaagaaat cgttgaattg gcagaaggat ttgctaaaga aagtggcgca catgttctca
    13501 tcactgaaga tgctgatgaa gcagttaaag atgcagacgt tctttacaca gacgtttggg
    13561 tatcaatggg tgaagaagac aaattcgcag aacgtgtagc tcttcttaaa ccttaccaag
    13621 tcaatatgga cttagttaaa aaagcaggca atgaaaactt gatcttccta cactgcttgc
    13681 cagcattcca cgatactcac actgtttatg gtaaagacgt tgctgaaaaa tttggtgtag
    13741 aagaaatgga agtaacagac gaagtcttcc gcagcaagta cgctcgccac ttcgatcaag
    13801 cagaaaaccg tatgcacact atcaaagctg ttatggctgc tacacttggt aacctttata
    13861 ttcctaaagt ataattttag ataataaacc gtctaccaac agctatgagg gctgcgacta
    13921 atagctttag tccggtcctc ttttatgtaa tggtaatcta ttatttctta taaaatatgt
    13981 gaaaaatcat taaattgaaa tctaaacgca ttctattgag tgtgataaag gagaatttat
    14041 ggcaaatcgt aaaattgtag tagctttggg aggaaatgcg attctttctt ctgacccatc
    14101 agcaaaggct caacaagaag ctttagttga aacagctaag catcttgtaa aattgattaa
    14161 aaatggagat gatttgatta tcactcacgg taatggacct caagttggga atctcttgct
    14221 ccaacatttg gcatcagact ctgaaaagaa ccctgccttc ccactcgact cacttgtcgc
    14281 tatgacagaa ggtagcatcg gtttctggtt gaaaaatgct ttgcaaaatg ctctcttgga
    14341 tgaaggcatc gaaaaaaatg ttgcctctgt tgtaacgcaa gttatcgtag ataaaaatga
    14401 tccagctttt gttaacttga gtaaaccaat cggtcctttc tattcagaag aagaagcaaa
    14461 agcagaagcc gaaaagagcg gagcgacttt caaggaagat gctggccgtg gctggcgtaa
    14521 ggtcgttgcc tcaccaaaac ctgttgacat caaagaaatt gaaaccatcc gtactctttt
    14581 aaataatggt caagtcgtcg tagctgcagg tggtggcggt attcccgtcg tcaaagaaaa
    14641 caatggacat ttgactggtg tcgaagcggt tattgataaa gacttcgctt cccaacgttt
    14701 ggcagaattg gttgatgcag acctcttcat cgttttgaca ggtgtagatt atgtatttgt
    14761 taactacaac aagccaaacc aggaaaaatt ggaacatgtg aatgttgccc agctggaaga
    14821 atatatcaaa caagatcagt ttgcaccagg tagcatgctt ccaaaagtag aagcagctat
    14881 cgcttttgtc aatggtcgtc cagaaggaaa agcagttatt acttcccttg aaaatctagg
    14941 cgccttgatt gaatctgaaa gcggaacaat tattgaaaaa ggataagttg ttttactaat
    15001 aagatgtatt ctatttctag tatctttata tcaaattaga aattattctt gaaaacatgt
    15061 acaatatttc aaaagatact agttttagac tttaatatgg taaaacaaat ataaatagaa
    15121 agcgttttct tgaatgttta tttaagaaag tagttggttt tttacacttt gttagacatc
    15181 aggaggaaaa acaaatgagt gaaaaagcta aaaaagggtt taagatgcct tcatcttaca
    15241 ccgtattatt gataatcatt gctattatgg ccgtgctaac ttggtttatc cctgcggggg
    15301 cctttataga aggtatttac gaggctcagc ctcaaaatcc acaagggatt tgggatgtcc
    15361 tcatggcacc gattcgggct atgctaggta ctcatccaga ggaaggttcg ctcattaaag
    15421 aaacgagcgc agcgattgat gtagccttct tcatccttat ggttggtggt ttccttggca
    15481 ttgtcaacaa aactggtgct cttgacgtag ggattgcctc tatcgtgaag aagtataagg
    15541 gccgcgaaaa aatgttaatt ttggtattga tgcctttgtt tgccctcggt ggtacaactt
    15601 atggtatggg tgaagaaaca atggccttct atccactcct tgtgccagtt atgatggccg
    15661 ttggttttga tagcctgact ggtgttgcaa ttattttgct cggttctcaa atcggctgtt
    15721 tggcatctac tctgaatcca tttgcgacag gtattgcttc agcgactgcg ggagttggta
    15781 caggggacgg tatcgtactt cgtctgatct tctgggttac cttgactgct cttagtactt
    15841 ggtttgttta ccgttatgcg gataagattc aaaaagatcc gactaagtca ctggtttata
    15901 gtactcgcaa agaagatttg aaacacttta acgtagaaga atcttcatct gtagaatcta
    15961 cacttagcag caaacaaaaa tcagttctct tcttatttgt gttgacattc atcttgatgg
    16021 tattgagctt cattccatgg acagaccttg gcgttaccat ttttgatgac tttaatactt
    16081 ggttgactgg tcttccagtt attggtaata ttgtcggttc atctacttct gcactaggta
    16141 cttggtactt cccagaaggc gcaatgctct ttgcctttat gggtatcctg attggtgtta
    16201 tttatggtct taaagaagat aagattatct cttccttcat gaatggtgct gctgacttgc
    16261 tcagtgttgc cttgatcgta gcgattgctc gtggtattca agttatcatg aacgacggta
    16321 tgattaccga tacaatcctc aactggggta aagaaggctt gagcggtcta tcttcacaag
    16381 tctttatcgt tgtaacttat atcttctatc tacctatgtc attcttgatc ccatcttcat
    16441 ctggtcttgc cagcgcaact atgggtatca tggctccact tggagaattt gtaaatgtcc
    16501 gtcctagctt gattatcact gcttaccaat ctgcttcagg tgtcttgaac ttgattgcac
    16561 caacatctgg tattgtgatg ggagctcttg cacttggacg tatcaacatt ggtacttggt
    16621 ggaaattcat gggcaaactc gtagtcgcta ttattgtagt gaccatcgcc cttcttctcc
    16681 ttggaacctt ccttccattc ctataaaata gtgagtgagg tgattccatg aaaatagata
    16741 taacaaatca agttaaagat gaatttctta tatcattaaa aaccttgatt tcctatcctt
    16801 cagtactcaa tgaaggagaa aatggaacac cttttggaca agcaatccaa gatgtcctag
    16861 aaaaaacttt agagatttgt cgagacatag gtttcactac ctatcttgac cctaaaggtt
    16921 attacggata tgcagaaatc ggtcagggag cagagcttct ggccattctc tgtcatttgg
    16981 atgttgttcc atcaggtgat gaagcagatt ggcagacacc gccatttgaa gcaactatca
    17041 aagacggctg ggtattcgga cgtggtgtcc aagatgataa aggcccttcg ctcgcagctc
    17101 tctatgcagt aaaaagcttg ctggaccaag gtattcagtt caaaaagcgc gtacgcttta
    17161 tctttggtac cgatgaggaa accctctggc gctgcatggc acgctacaat accatcgaag
    17221 aacaggccag tatgggcttt gcacctgact catcttttcc tctgacctat gctgaaaaag
    17281 ggcttctaca ggtcaaactt catggccctg gatcggatca actagagctt gaagtaggag
    17341 gcgcctttaa cgttgtacca gacaaggcca actaccaagg tcccctctat gaacaggttt
    17401 gtaacggtct caaagaagct ggttatgatt accaatccac tgaacaaacc gtaacggttc
    17461 tcggagtgcc aaagcatgct aaggatgcta gtcaaggtat caatgctgtc atccgactag
    17521 ctaccattct tgctcctctc caagaacacc ctgctctcag ttttcttgca acacaagcag
    17581 gtcaagacgg cacaggaaga caaatctttg gtgatatagc agatgaacct tctggtcacc
    17641 tatcctttaa tgtcgcaggt ctcatgatca atcatgaacg ttctgaaatc cgtattgaca
    17701 ttcggactcc tgtcttagct gacaaggaag aactagtaga gttgcttaca agatgtgcac
    17761 aaaactacca actccgctac gaagagtttg actatctagc gcctctatac gtcgcagaag
    17821 acagtaaact cgttagcaca ctgatgcaaa tctaccaaga aaagactggc gataacagtc
    17881 ctgctatttc atccggtggt gccacttttg ctcgcaccat gccaaattgt gtagccttcg
    17941 gcgccttatt cccaggagcg aagcagacag aacatcaggc aaatgaatgt gccgttctag
    18001 aagatttgta ccgtgctatg gatatttatg ccgaagccgt ctatcgactt gcaacttaat
    18061 caggcaactg tttctaccaa aaaaaatcga ccgattaatg aactgcaccc caaaag
//
LOCUS       DAVBHS010000027        17999 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVBHS010000027 DAVBHS010000000
VERSION     DAVBHS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17999
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.027"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(62..859)
                     /gene="proC"
                     /locus_tag="VQ651_000662"
     CDS             complement(62..859)
                     /gene="proC"
                     /locus_tag="VQ651_000662"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET2753688.1"
                     /translation="MKIGFIGLGNMGASLTKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(863..2125)
                     /locus_tag="VQ651_000663"
     CDS             complement(863..2125)
                     /locus_tag="VQ651_000663"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET2753689.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAVSFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            complement(2135..3265)
                     /locus_tag="VQ651_000664"
     CDS             complement(2135..3265)
                     /locus_tag="VQ651_000664"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET2753690.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMVEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRFQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(3348..5228)
                     /gene="cbpE"
                     /locus_tag="VQ651_000665"
                     /gene_synonym="pce"
     CDS             complement(3348..5228)
                     /gene="cbpE"
                     /locus_tag="VQ651_000665"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HET2753691.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSAYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGASAKNWKKIDGIWYYFNKENQMEIGWVQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTGGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SE"
     gene            complement(5231..6118)
                     /locus_tag="VQ651_000666"
     CDS             complement(5231..6118)
                     /locus_tag="VQ651_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2753692.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(6108..6569)
                     /locus_tag="VQ651_000667"
     CDS             complement(6108..6569)
                     /locus_tag="VQ651_000667"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET2753693.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMFLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(6566..7474)
                     /locus_tag="VQ651_000668"
     CDS             complement(6566..7474)
                     /locus_tag="VQ651_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET2753694.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            7467..7664
                     /locus_tag="VQ651_000669"
     CDS             7467..7664
                     /locus_tag="VQ651_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2753695.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            complement(7716..8387)
                     /locus_tag="VQ651_000670"
     CDS             complement(7716..8387)
                     /locus_tag="VQ651_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2753696.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(8441..8662)
                     /locus_tag="VQ651_000671"
     CDS             complement(8441..8662)
                     /locus_tag="VQ651_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HET2753697.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            8934..9743
                     /locus_tag="VQ651_000672"
     CDS             8934..9743
                     /locus_tag="VQ651_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2753698.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(9924..10799)
                     /gene="aguB"
                     /locus_tag="VQ651_000673"
     CDS             complement(9924..10799)
                     /gene="aguB"
                     /locus_tag="VQ651_000673"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HET2753699.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKLLAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYNLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(10809..11894)
                     /gene="aguA"
                     /locus_tag="VQ651_000674"
     CDS             complement(10809..11894)
                     /gene="aguA"
                     /locus_tag="VQ651_000674"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HET2753700.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVV
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(11891..13018)
                     /gene="nspC"
                     /locus_tag="VQ651_000675"
     CDS             complement(11891..13018)
                     /gene="nspC"
                     /locus_tag="VQ651_000675"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HET2753701.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(13018..14277)
                     /locus_tag="VQ651_000676"
     CDS             complement(13018..14277)
                     /locus_tag="VQ651_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HET2753702.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMLD"
     gene            complement(14274..15134)
                     /gene="speE"
                     /locus_tag="VQ651_000677"
     CDS             complement(14274..15134)
                     /gene="speE"
                     /locus_tag="VQ651_000677"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET2753703.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEYDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            15515..16104
                     /locus_tag="VQ651_000678"
                     /pseudo
     CDS             15515..16104
                     /locus_tag="VQ651_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16070..17545)
                     /locus_tag="VQ651_000679"
     CDS             complement(16070..17545)
                     /locus_tag="VQ651_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET2753704.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT         5301 a         4134 c         3281 g         5283 t
ORIGIN      
        1 agtttgaatt tgattttcga agagtatcag ccaccataaa agaggcagat aaaactacct
       61 cttatttacc tagttcttgt gttcgtttat aggcttgatg aactgcctcc atgactgtgc
      121 ctcgaaaagc atgcgcttct aggcttgcta cgccagcaat agtcgaaccg cctggactac
      181 aaacttgatc tttcaaaact ccaggatgtt gctggctttc taggaccaat tgcccagctc
      241 ctaccacagt ttgggctgcc attttcaagg ctgtttctcg tggtaatccc gtctgaacac
      301 ctgcatctgc caaggcctca ataaagagat agacaaaggc tggtccacaa cctgcaagac
      361 ctgtcgctgc attgattaag ctttctccta attcaaccaa gagaccagcc ttggctaaaa
      421 gctgacaaaa gatctcactg tcctcagccc tgcaattagg agacaaggca taactaatca
      481 ctccttgccc gatagaagca ggggtattag gcatcatacg aataattcgg tgttggcttg
      541 ggataagact tgctagtttt tctaaggtca atccagctgc catcgaaatc aaaagaagac
      601 tttctctttt ttcaaggatg gtctggtatt gagaaagcag ttcagaaaac tgagcaggct
      661 taacacctag aaaaatcaca tctgcttctt cgaatatttc ttcattgtta gaagtctgac
      721 caccgaagtt agcgatgaaa gcatccacct tgacttgact acgattggca aggagaatct
      781 catctgacgg tctagcctgc aagacagact tggttaaact agcacccata ttccccaaac
      841 cgataaatcc aatcttcatc tcttactccc ttatctgtcc atcaccagtg accacatact
      901 tgtagctagt caattccttc aagcccatag gaccacgcgc gtgcaatttc tgagtagaaa
      961 tccccatttc acaaccaaga ccaaattgtc ctccatctgt gaaacgagtt gaggcattaa
     1021 catacaccgc tgcagagtcc acttgatctg taaagtatgc tgcagcttca gcattttccg
     1081 tcacaatggc atccgaatga tgggtactgt gggattcaat atgcgcaacc gcttcttcta
     1141 aactgctcac aaccttaaca gctagaatat agtctaaaaa ctcggtatca aagtcttgtg
     1201 cttgagcagc ttgacctgaa acaaactggc ttgctttgct atctaggcgg aattgaattg
     1261 gttccaaccc agcttctttt cgatctgcaa ccaacacttg ctccaagcga ggaaggaagc
     1321 ttactgcttt gttttcatga accagcagaa cctccatggc attacaaaca gaaggacgac
     1381 tggttttagc attgttgatg atagacagcg ccttgtcttc gtctgcatcc ttatccacat
     1441 agacatggac aatcccagtt cctgtctcga taacaggtac aatcgcattc tcaaccactg
     1501 cattgatcaa gccagctcct ccacgaggaa tgagaaggtc tagatagccc ttggccttca
     1561 tcatagcata actactttca cggctagtat cctccaccag ttgaatcaca tttggatgaa
     1621 tagtagtcgt ctccaagccc ttcttcaagg ctgtgacaat ggcatgggtt gtttgatagg
     1681 catccttacc actacgaaga acaaccgcat ttccactctt aagagtcaaa gcagccgcat
     1741 cagacgtcac atttggacgg ctttcataga taataccgat gacacccata gctacacgtt
     1801 tttttgtgat aaccaaacca ttttcaagct gacttgtttc taaaacttca ccgattggat
     1861 ctggtaaggc aaccacttca cgaattcctc ttgccatcgc ttctatacga tctgcatcca
     1921 aataaagacg atccaacatc acatctgaga ttttcccctt agccgctgcc atatcgaggg
     1981 cattagccgc taaaatttcc tcagtagcag ccactaagtg atcagccatg gctagcaagg
     2041 cttggttttt cacttcttca ctagctgtgt taatcgattt tttaacagcc tgtacctgtt
     2101 caaattgttc ttgtctactc accatagttt acctctaaaa ttctgtaaaa agtagttgga
     2161 tttcaggagt aatggaaatc cagtcgtcac ggtaaatcaa gacacccttg gctttttgaa
     2221 aacgcaacat atcctccaaa gcagatgctc caaattgcac gcgtcctttt ccaagtgatt
     2281 ttccactttc cttatcaaat actgtcacga tatcaccgta agaaaagact ccttctgctt
     2341 caacgatacc agataagaga agactctttc catgttgaga gagagcttcc gcagcccctt
     2401 tatcaaccca aatagaacct tgactctgag catagaaggc aagccattgt ttctgggtac
     2461 gaagcccctt ctcttgagca acaaagtaag aaccatcctc ggtctcctct gccgcctcaa
     2521 ccatggaatc tgatttcaag gatgagcaga tataaacagg aactcctgat tccgtcgcga
     2581 tagttgcagc cttgattttg gttaacatac ccccagttcc gtttgacgaa ccagctccac
     2641 cagccatatc aataatctca cgattgatgg tctcgattct ctccaagcgt ttggctcttg
     2701 gatctgaatt aggatttcca gtatagagac cgtccacatc tgtcaagaaa actaaaaggt
     2761 ctgcttggac catcgccgct acttgagcac ttagagtgtc attgtcccca accttgagct
     2821 catcaataac gacactatca ttctcattga tgataggaat tgccccacgg ttgagcaaaa
     2881 ccgacaaagc ctgatgggca tttttataac gacgcttatc cacaaagtca tcttgggtca
     2941 gcaagatttg tgcagaaacg atttgacgca agagaagatt ggtcgtatat tcttccaaca
     3001 aaagtccctg ccctaccgct gctgaagcct gtttatcagc aattttagtc ggacgctttt
     3061 taaatcctaa ggctccaaaa ccagccgcaa tggcacctga agacaccaaa atcaactcat
     3121 gaccagcctc gtgcagcata gccaactgct gggtaatatc ctttacctta ctacgtgata
     3181 aacttccatc ctcatttgtc agagaagaag tgcccacctt aaagacaatc cgtttgtatt
     3241 tcatagtctc actccgatta ttcatattta tccattataa catgaataac aggaaataga
     3301 aaagagttga taggaaaaag aaggccctaa aaccttcttt tctactacta ttctgattcc
     3361 gatttgttta cactcgtttc agagctttgt gaagttgagg atgtcaaatc ttgactagtt
     3421 gaagaatttg taacactttc atgttgactc gtttctttat tttcaagatc ctttttcact
     3481 acttctttgg attcttttat ttctgaatga cttgtagttt taattgtagc agatttctcg
     3541 atcggaacat ccactaacca ggcaccactt gaatccacag tatagccttc tggtgtctta
     3601 ccattcacaa gtaattgacc attttctttc aagaagtacc atttatcctt gtctttaatc
     3661 caacctatag ctcgtgaacc acctgtcttg tagaaatacc aatcatttgc ctttttaagc
     3721 cagccttgct tcatagctcc tgattcttct agatagtaat gatgtccagc aacttctatc
     3781 tcacctgtct tcatgatacc agtagaatcc atatagtacc aggcttcttt atcttttacc
     3841 cagcccgttt tcatttcccc tgatggagca aagtaatacc attgcccctt atattgaagc
     3901 caacctgtct tcatagagcc atcaacatcc aaataatacc actgctcttt atcttgaacc
     3961 caaccaattt ccatctgatt ttctttgtta aaataatacc agattccatc aattttcttc
     4021 caatttttag cagaagcacc agagtctgtc aaatagaacc aatgattgtt ccattttttc
     4081 cattgattct gtaacaagat acccgtttgg ttaaagtaat accattcacc ttcgatttca
     4141 ttccaaccga cagcatactc tcctgtagaa tcaggcgctt gataccacca gttcccatat
     4201 gcactcttat gccaaccagc ttgaaaactt ggaatcggct tgtaggatgt tgaaatattg
     4261 acaaaaccgt cttttcgaat atcaaaaact gttgcatcat agtctttgct ggctgcgttg
     4321 attctctcaa ttcctcgttc tttgagccaa ttaacatact cactatcaac accatttttc
     4381 caaggtagac tatccgaagt ttgaacaatc aaactcggac tcaaattttt aatgaaatcc
     4441 ttggtatttg atttgttggt atcatggtga tgattaaact tcatcaaatc aacttttcca
     4501 atgagaggac catacttgtc ttctgctcca tgaacattat ctaaatcgcc cccaaggtaa
     4561 attttcttgc cattgacttt caccacgcta atcaaggaat tggaattgtc atcccaaatt
     4621 ttctttaatt cacccgatga atcagtttca ttttcataat tatagagctg aatatccatg
     4681 tccccaaact gaaaatgagc atccccttgt gtgatatttt gaataactga aacacctttt
     4741 tctgcagcag tctgtaaaac cttatcatag ccatacagat tatcccatag acgttcagaa
     4801 ttagtaatac gactatcact atatttctta agatagactc ggtcaactgg ataggtagac
     4861 agtaattcat caacatttcc aatatgatca ctgtgggtat gggtcaccaa aataaaatca
     4921 agtttttgga cacccaattc cttcaaacga cgaaagacac ggtctgttag aacatgctta
     4981 taagacgttt caattccttc tctccatgga taacgagaat cacttccatc tgggaaatca
     5041 taatcttctc ctgtatccac catggcaaaa tgtccattgc tttcaagaat aatcgcatca
     5101 ctgccacctt cttgaacatt gataaagtgg attttatttc ctgaactttc ttgagcctga
     5161 acattcccat accatgacaa acctaaaaaa gcgcctacaa gtgctaaact agttaatttc
     5221 tttttcattc ttactttctc aatctctcca aggtttcctt aaaaatctct gggatatctg
     5281 ctttaaattc caaggtctta cctgttctcg gatgagtaaa acctaaagtc ttggcatgaa
     5341 gaaattgtcc atgtcctttc aaagtcttgc gaggaccata gacctcatca ccagcgactg
     5401 gatggccgat ataagccatg tggacacgga tttgatgagt gcgccctgtc tccagttgca
     5461 actctactaa gctataatcg ccaaagcgtt ccaagacgtg aaaacgcgtc actgcaggct
     5521 tccctttagc agttacagcc tgtttcttac ggtctttttc actccggcca atcggcgctt
     5581 caattacacc acgatcatta ggcagatttc catgaacaat cgcccaatat ttgcggagag
     5641 actttttatc tttgagttct tgggcaagta ctagatgcgc atcatcgttt ttagcaatca
     5701 tgagaagacc tgacgtatcc ttatcaatac ggtgaacaat ccctggacgc agaaccccat
     5761 tgatacccga caagtcctta atatgataca tgagggcatt tactagggtt ccactggtat
     5821 gaccagcact cgggtgcaca accattccct gaggtttgtt aacgacagcc acatcctcat
     5881 cttggtagac tatttctagc ggaagatcct cagccacata ctctaatacc tctggttctg
     5941 gcacatggta agtgacgaca tcaccctctt ggactgtgta tttagctttc ttgacttgac
     6001 cattgaccaa gacctggcct gatttaattt gttcattcgc gagactacgt gataattctg
     6061 acaaatctga caaagcctta tccaaacgca gaccaccagt ttcaatttta atttccattt
     6121 atttcctctt ttagcattgc aatcaataaa ataatcactc caaccgtcag atagctatct
     6181 gccacattga aaattgcaaa gttgataaag tcaaggtgga acatatccac aacaaagccc
     6241 tgactgaccc tgtcaataaa gtttccaaga ccacccgcga ttattagagt caaacccaag
     6301 aacatccaga atgagtcctc catgtgttta tgtaaatacc aaatggcacc tatcacgaca
     6361 accagagtaa tgacagcgaa taacagctgc tgatcttgta agatagaaaa ggctgcacct
     6421 cgattttgca ggtaggtcaa gctaacgaaa ttgggaatcc aggagcgcac ttcacccagt
     6481 ggaatctgct ggacgatata ggatttgacc aactgatcca gcccaattaa aagcagtaca
     6541 atgactgcca ctattgctct ttttttcatg atttcctctt ttgatcaaaa tattcttgca
     6601 tgacttctac gaagagagtc ccagcttgac taagctccac ttcttcacgt ttaacataga
     6661 ccatgcggtt atctaggtta tccttgagac gaataactgt aatgccatta acactgtcac
     6721 tatctaaaaa tccagaacct gtcgcatagg cgtccgtccg ctccaaaata ccattcaagg
     6781 tggcacggtc tgtcacatta aacatctgtg agctagcgct ggtatcgaca aagttctctg
     6841 aataataaag gtactcgtct ttctcttgag tgaaacgaac cgttggtaaa tccgctaaat
     6901 cctccatgac taattcctct ttctgggcta aaggatgacc ctcacagaga taaatatggg
     6961 tatggaaagg aatcaattcg atgacctcca gacctaactt ttcaacccgt tgcataatcc
     7021 cctttttatt ttgattgttg aggtagataa tcccaatctc actatgccct tgcgccactt
     7081 catctaatat ttgaacagta gttgattcaa aaatacggaa gttcttatag tcaggatagc
     7141 gctctgaaaa ggccgtaata gttggtggta agaagtcata gtgctggcta gcaacggaaa
     7201 attcatcttt ttcttcttca ggattggcat actgattttg aaaaatatca aatcctttaa
     7261 ccaattcttg cgctttttca taaaattcca tcccacgacg ggtcaagaaa gtccctgagc
     7321 tggtccgacg gaaaatctta aagcccaact ctttttccaa atcacgaaca gaaatagaca
     7381 gactcggctg actaacatac atcttttcag cagcttcacg aaaagtacca ctattggcaa
     7441 tagccacaac atagcgtaat tgttgaatgt tcatcttcta cccccaactt ctttatcttt
     7501 tcattatacc atattttaga agttttccaa aaaggaaaaa agaacatcct attcttctta
     7561 actatcttca ctatctgcct ttctcacgcc aatcttattt tcaaaatcaa ttcaaaaaaa
     7621 taagtggcta cacaccactt cagtatcgga aagaaaaacg ttgactattt gtgaaaaaag
     7681 aaaatgccgg aaaattccga cattttttta gtaagctaac ttcctgaaaa taggagtaac
     7741 caaacatccc agaaggcagt gatattgatg agaatatgaa ctagtattgg atagtagaga
     7801 tttttagtca tgcgatacaa tagagctaaa atcagacctc cactagcata aatgaaaaaa
     7861 gcaagaggag ttaaagcaaa attgatatga atataaccga aaataatagc agaaagcaaa
     7921 acatctccgt accaaggtga gtttttgaaa aaggttgtca taagcacacc tcgataaatc
     7981 aattcctcag caataggggc gatgaagcaa acgatgagca agaaataagg gaactcctgt
     8041 ctccccatca tttctaccgt ttcattcaaa gaaatctgat ttgaagacag ggatatgaaa
     8101 tacgaaaaga ggaagtcaga catatatgaa atgatgtagc ccagtaaaag gtagatgaag
     8161 tacctcagct gccacttgaa atgaaaaatt ttctttcctg caaaaaccag tagatagatg
     8221 accgctaaga aaagaactcc gctctccatc aaaagcagaa tctgaaaaaa ttcacgactt
     8281 gctggtagat agggctttgc gaggtgattg aaaactctcc aaaaccaagt tgatttgtaa
     8341 aaaattaagg acaatgctag taaaatttga atagcccgtt ttttcatatt aaattctctg
     8401 ctttctccac ttatttcttc atattactaa taaattttac ttaaaatctc gcagcacttc
     8461 ctttgcaaag atgattgcct cctccaatat atcctaaatc ataggtgcct ctgggacaaa
     8521 atccatgaac cggcagagtt aatactggtt ctatttccca atcgtaccta ccattaggat
     8581 ttttaccata tgaatctgct ccaccttgta tcagctctaa ttcttcattt gttagattta
     8641 tattttttgt cattaaaatc atgatatatt tcctccatta ttcttcagat tagttgagta
     8701 atctgatact catacctact tacaaaaaaa ctattatatt aagttggttt tttaatttgt
     8761 ctagttgcaa cattgacaaa catagtatag catatctttg cgaaatatcc tcttcaaatc
     8821 atgaattgtc atcaaaacat cttaaactat aaaatcaatt agtctcaagc tttctatcaa
     8881 tttcttctcc aaaatatgct ataataatag caaaagataa agaaggaaga cctatgatta
     8941 aactactagc cttggatatg gacggcaccc tcctcaatga agccaaggaa atcccacaag
     9001 ctcacattac tgctattcac aaagccattg aaaaaggtgt caaactggtt ctctgtacgg
     9061 gtcgccccct tttcggtgtc ctcccctact acaaaaaact gggactcgac ctccagaatg
     9121 agtatgtgat tgttaacaac ggttgttcaa ctcaccagac tagcgactgg ggcttggttg
     9181 actggcaaga acttagtcca gctgacatcg aatacctcta tgaccttgct gaaaagagtg
     9241 atgttcagtt gacacttttt gacgagtcac attattttgt tctcggtgga aagcccaatc
     9301 aagttattga aaatgatgct aaactagtat tttcagacct gactgaaatt tctcttgagg
     9361 aagcgactag tggaaagttc cggatgttcc aaggtatgtt tttaggaaca aaagaacaaa
     9421 cagacgattt tgagcagcgt tttgctgaag agctttgcca acgattcagt ggagttcgtt
     9481 cgcagcctgt catttatgaa gcaatgccac ttggaacgac aaaggctact gctctttcac
     9541 gactagctga gattttgaag attgattcct cagagattat ggccatgggc gatgctaata
     9601 acgatatcga aatgctccag tttgcagggc ttgggattgc aatgggaaat gccagcgatt
     9661 atgtcaaatc tcttgcggat gccgttacct caagcaacga agaagacggc gttgcgcgtg
     9721 ctattgagaa atatattcta taaaaaagaa aagcaaatag acagaagtta ctgctatttg
     9781 cttttttgct aatattttaa aatacggact aatcaataga gaagaatagc gaatcataaa
     9841 aatcgattta ctagatgcca tagtatctta tagttgctaa taagaagtta gtctagcaga
     9901 atgaatctct catttctccc acactaatct gtaatttgtc tatacatttc tggtcttcta
     9961 tctcgaaaca agccccagtt taggcgttca cttgctccct tgtctaggtt ataagtagct
    10021 aacagaacag cttcttcttg tctttcagct cgttctagaa tagctcctgt ttcatccgtc
    10081 ataaaggagg aaccgtagaa gtcaagactg gaactctgtc cgccattttc ctcactagga
    10141 gtaacctcct ctaaaccata acgattggct gcgatgactg gaacaatatt cgctgctgcg
    10201 tgcccttgca tagtacgttg ccagtgacca caactatctg tatccaaaat tggctctgaa
    10261 ccgatagctg taggataaaa gagcaattca gcaccattca atgcaagaca gcgcgctgtt
    10321 tcagggaacc attgatccca acagataccg ataccaatct tagcatagcg agtattccag
    10381 accttgaaac cagtgttacc aggcgtgaaa tagaattttt cttgataata atggtcatct
    10441 ggtatatggg tctttcgata aacgcccagc acttccccat ctgcatcaat gacggcaata
    10501 gagttataca agacattacc atctttttca tagaaactga ttggtaaaac aacttgtagt
    10561 tccttagcaa gcaacttaaa atgctgaatg gcagtatttt ccgctacaga ttgggcatac
    10621 tggtagtagt catactgacg ttcctgacag aaatagggat gttcaaacaa ctcgggcaag
    10681 agaataattt gggctccttg ctcagcagcc tgacgtacta aacgctctgc ggtttggata
    10741 tttgttgcca catccttagc gcattgcatc tgaatggttg caactcttac atttctcatc
    10801 tttttctcct attctggaat ttgttgggtg atacagtgga tattgccacc acctaagaga
    10861 atatctctgg ctggtattcc gacaacttta cggtctggga aacacttgct gaggatatct
    10921 aaggccactt ggtcgtttac atcctcaaac tgtggaacca agacagcctt gttggcgata
    10981 taaaagttta cgtaggaagc tgctagtcgt tcacctgcgt atcgctcttc ttctccttct
    11041 tcatagatgt agcctggcaa atcttcttct gtcacaactt gtcgaactgc agggataggc
    11101 aatttatgaa tggtgaagtg acaacctttt gcatctgttt cctgttctaa gagttcgaga
    11161 tctgcttttg acatggcata ctggggatca ttttcgtcat ctgtccaagc caaaacaacc
    11221 tcagcaggac caacaaaggc aacaacatta tcgacgtgtt cattggtttc atcctgataa
    11281 ataccataag gaagccaaat aactttttca gcaccaagac tttctaataa tgtgttttca
    11341 atctcctctt tagtcaagtt aggattgcga ccaggactaa gcaagcaact ttcagttacg
    11401 agaatagttc cttgaccatc gctatggatt gcgcctcctt ccagtacaaa aggtttagca
    11461 tcatagacag gcctttccaa ggcctcagca aaacgactgg ctacttggtc atcctcttca
    11521 taatcttgat aaagaccatc ataggtgcct ccccaagcat taaaggccca atccacggct
    11581 aatttcttac ctttatcatt gacgagaatg gttgggccag tatcacgcgc ccaggcatca
    11641 ttggtgggaa tgtctaaata aacaaccttg tctccaagat aggattgggc ttcagataga
    11701 taggcctgct ccaccaaaag atagactctt tccccttctg ctatggtctc gatgatctga
    11761 gtaaatgctc ttttagcagc ctttccttga aaaggccatg atcctggtcg agtcggccat
    11821 atcatgaggg taccatgatg gggttcgtac tctgctggca tgtgatagcc taattttttt
    11881 ggactgtcca tcatgataat ctccctttaa agtcttgata gccaaaagct ttgagtaagc
    11941 tacagtctcc ctgttcgtcc atgagataga gacttggcaa tccaatacca ttaaaggtat
    12001 tatttttgac aaaagaataa atggccatgt cttgaaaata aagtctgtct ccgatttgga
    12061 ctggattttc aaaactataa tcaccaatca catcgccagt cagacaggta ttagaagaaa
    12121 gtctgtaggt atgggctttt tcctgtgact caaagccatt tctcaaaggt ggacgatagg
    12181 gcatctcaag tacatcaggc atatggcagg tcgcagaggc gtctaaaacc aagatttcca
    12241 taccgttttc tacaatatct aatacctcag ttgctaaata acccgcatta agcgcaatgg
    12301 cttcaccagg ctcgatatag atttcaagat tgtaagtttt tcggatacgc ttgatttctg
    12361 aaatcagcaa atccacatcg taaccttctc ttgtaatatg atgaccacca cccatattga
    12421 gccattttac ctcatgtaag tagggaccaa actgttcttc tactgctttc aaagttgttt
    12481 gtaaatcatc tgctccctgc tcgcaaaggg tatgaaaatg aagtccgtca accaaatcta
    12541 gcaaatcgct cggaatcttg tctatagtaa ctccaaagcg agaaccaggt gcacaagggt
    12601 catagagcgc gtgatctcct tgagttgaac actgagggtt gaggcgcaaa ccaacactga
    12661 caccagcctc tcgacaacga gccccatgct tacgcaactg tctctctgag ttaaagacga
    12721 tatggtctgt tatcccaagc aattcctcca tgtcctcatc cttgaaagct ggtgcaaaaa
    12781 catggacttc accaggaaat tcttcccttg ccaatttggc ctcatagagt ccactagctg
    12841 tcgtacctga tagatactgg ctaatcaagg gataagtttt gtagagggaa tatgccttct
    12901 gggcaagcaa gaccttgcaa ccggcctctt cttgtacata ttgtagaatg cggcaattag
    12961 cttctaactt ggccaagtca ataacatagg ctggtgttgg tacttgttct aacttcatta
    13021 gtccaacatt tgtggatttt caaccacaac ccatggcaaa ccatactcat tcaaagcttc
    13081 catgaatgga tctggatcta actcctcaag gttatacact ccagcttgtt tccaagttcc
    13141 gttcatgact aattttgtcc caatcatggc tggaacgcct gtcgtgtatg aaattgcttg
    13201 tgacccgact tctgcatagc actcttgatg gtcgcaaaca ttgtagatgt agatagtctt
    13261 ttcaacgcca tctttgacac ctgtaaagat acatccaata ttggttttac ctacagtacg
    13321 tggaccaagg ctagcaggat ctggaagcaa ggctttcaaa aattgaattg gaacaatttc
    13381 ttggccgtta aagttaatgg tatccgtacg aaggagtcca acattttcaa gacatttcat
    13441 gtgcgtcaag taagattgac caaaagtcat aaagaaacga atgcgtttga cacctggaat
    13501 gttcttggcc aatgattcga tttcttcatg gtgaaggaga tacatatctt tttgtccaac
    13561 ttgagggaaa tcatactcac gcttgataga catagcttcg acttcgaccc atttcccatc
    13621 ttcccagtaa gaacctggcg cagaaacctc acggagatta atttctggat taaagttggt
    13681 tgcaaatgga taaccgtggt caccgccatt acagtctaaa atgtcgatat aatggatttc
    13741 atcaaaatag tgtttgaggg cataagctga aaagacacta gttacacctg ggtcaaaacc
    13801 agaaccaaga agagcagtca agcctgcttc tttgaatttc tcttgataag cccactgcca
    13861 tgagtagtca aagtaggctg taaaaccaag ttccttacaa cgtttttcgt agatagcacg
    13921 ccactcaggg tcttctgtgt cttctgcttc gtagttggct gtatcgatat agtgaacacc
    13981 tgttgccaaa caagcatcca taatggttaa atcttgataa ggcagagcta cattcaaaac
    14041 agcttctggt ttgtagcttt caatcagagc aatcacttct tcaaccttgt cagcatcaag
    14101 agcagctgtc tcaatctttg tacttgtttt gccttccagt ttagctttca agtcatcgca
    14161 ttttgacttg gtacggctag caatcataat ctctgtaaat gtttcgctat cttgacaaat
    14221 ctttgaaata gcaacttggg caacgccccc acaaccaata actagtaaac gactcatttt
    14281 tttccttcct cttcttctaa aatgtcctca acatacttgg gcaacataaa ggctcccacg
    14341 tgtaagtttg cagtgtagta ttctgtgaaa agctggcgtt ttttccagcc ttccttgtca
    14401 aaatctttga cagggtggta ttttttcgat gcaaatccaa acaaccaata gccagctggg
    14461 ctagttggaa tatgggcctg ataaacccga ctgattggaa aggcttgatt gaccttgcgg
    14521 tgcatgcttc ggcaggccga ctcatcctcg tcaaagaacg gactcccatg ctggtaaatc
    14581 atgatgccgt cttccttcag agctcgataa ctattgccgt agaattcctt ggtaaagagt
    14641 ccttccgtat ggccaaatgg atctgtcgca tcgttgatga taatatcata gtcatattcg
    14701 cagtttcgca aaaagcgtag cccattttgg tagtaaatgg taacacgagg atcatctagg
    14761 cctgcagcaa agtctgggaa atactcacga cagacctcga ccaacatctc atccggttcc
    14821 acgatatcga tttgttccag ttcaggatag agtgttaata cttgggcaac accgccgtca
    14881 ccacccccaa taaccaatac tttctttgga tttgggtgga cagccatggg aacgtgaacg
    14941 gtcatttcat tgtagacgaa atcatccgca tctgagaaca agacatggcc atttaaaatc
    15001 agtattttcc caaaagctgg cgtatccaag acttcgatat cctgccattc actttttcca
    15061 gcgtaaagtt gcttggctgt tctcagagac aatttgacat ctggagtatg aacttcagaa
    15121 aaccataaat ccatctattt ctccttatat tatatttagc gcagttagct actgccaaag
    15181 cccaagtggt atacttggaa taagccactg tggattagtt catttcctcc aatcgcttga
    15241 cgatttccga aagggtctaa ccacagcttt ttgtttcaat tggtaatgag tttgataacg
    15301 catagacgtt ttatctcgtc taaggtaaca aggcaaaaag ttcgtggata accaagtaga
    15361 cttgaaatac tatatctgga ggtactccca tgctttatgt gggcattgat gtcgctaaaa
    15421 ataaacacga tgttacagcc ttgaatgttc gaggaaaact gttcttaaac cactcacttt
    15481 ttcaaataat aaagctggtt ttgaactctt agatctgtct cttcgacaac tcaaccaaga
    15541 ctgtctcatc gctcttgaaa ataccggaca ttatgccttt aatctactga acttcctgca
    15601 tgaacaagga tataaagttt acacctacac tcctttgctc atcaaggaat ttgccaaatc
    15661 attatcactt cgcaagacta aaacggataa gaaagacgct catggcatcg ctcttaaact
    15721 tctttctgac cccaatcgtg aacaatttca acacgataat cggcaagtag aactaaaaat
    15781 actgactaga catattcatc gtctcaagaa aaaacagtct gattggaaag tacaatacac
    15841 tcgttgtctt gatatcatct ttcctgagtt ggataaaatc gttggaaagc attcagaata
    15901 tacctaccaa ctcttgacac gctaccctaa tcctcagaaa aggattgagg caggatttga
    15961 taagctgata gaaattaagc gattgaccgc ttctaattca agggcttttt gccgtgctct
    16021 aaaatcatcg ccaaatcatc aaattgaaat aagaaatagt ttagataaac tattgacttt
    16081 tcttatagtt tgtctttctc ttaataacgt tgatatgatt gacctctgga tcttccgtcc
    16141 cttggaggga gcaaccacgt tccttggcga attggatata gtcgacaatt tctcgtgtaa
    16201 tgcgttcacc aggagccaag ataggaatac ctggagggta acacataaca aattctccac
    16261 agacctgtcc aacagaatca tccaaagtta aactttttct ttctgaatag aaggcttctt
    16321 gcggagacag cactaactcg ggctgaatat attctcctgc tatcaaatct tttccatctc
    16381 ttgaatagag tctcttaata tcagccagag caccaaccaa gcgctcgatg tcttggatgc
    16441 ggtcgccgat ggaaatatag gccaagatat tgccgatatc accaaactcg atctgaatgt
    16501 cgtattcgtc tcgcaagagg tcataaacct cgatacctgt taagccaata ccctgagtgt
    16561 aaactgacaa cttagttacg tcaaaatcgc aaaccgaaac accgtctatt aactcttttg
    16621 agtaggcata gtagccaccg atagcattga tttcatgacg agcgtactcg gatagctcaa
    16681 tgacttcctc aaacgactct ttaccacgaa gggccaagtt gcgacgtgaa atatccaaac
    16741 tagccatcaa taagtaagag gcagatgtag actgggtcag gtttataatc tgacgaacgt
    16801 actcgggatt catctgctcc ccgattaaaa gaagcgaact ttgtgtcaaa ctcccaccag
    16861 acttatgcat ggaaactgct gccatatcag ctcctgcatc catagcagaa attggaagtt
    16921 taccagtgaa atgcaaatgc gctccatggg cttcatctac taaaaccatc atgccagctt
    16981 catgagccat ttctgtcaac ccctttaggt ctgaacagat gccgtagtaa gtaggattgt
    17041 tgattaggat agccttagca tctggatggt cctttatggc ctgtgctact cggtcatttt
    17101 caagacctaa agcgatacca atcttaggat ctacactcat ctcgatatag atgggaatgg
    17161 caccacatag aaccaacgca ttgatagcag atttatggac atttcgtggc agaataatct
    17221 tatctcctgc cttgcaggtt gccagaatca tagtctgcac cgatgaagtt gttccaccaa
    17281 tcattagaaa ggcatggcta gctccaaaag catctgcagc cagctcctct gcatcacgaa
    17341 taatcgaaat aggatggcct aaattatcca agggtttcat cgaattgaca tcaatgccta
    17401 cacatttttc tcctaagagt tcgacaagtt ctggatttcc ccgtccacgc ttgtgacctg
    17461 gaacatcaaa gggaacaatc cttttcttgc gtaacttcac caaggcctca taaattgggg
    17521 cttggttttg atctaactct ttcaaaacat actcctttca atatttttag gaccttatga
    17581 aaactaagga actaaaggtt gactcatgaa aaaagagcag gttttcacct gctctgcaat
    17641 cttctgacgt gtttattttg gtttctgttg agtatgtctg ttcccgatgt ttcctaactc
    17701 tctagtagca aatattacgt actttattga tcgtttcaca agatgacgtg tgctttcacc
    17761 acactaaaaa tttatgaatt aggacaagtg tcctaacatc aacttataaa agtaggcttt
    17821 taaccctatc aataatgtgg cttttcaacc acgcttcggc attcaaccct gcctgtccgt
    17881 aggcattttt tactcgggtt tatatttgct agaaaacatc atctcatttt ctaagcttta
    17941 ttactatatc atgtttttaa gaacttgtaa agtatttttt gataaaaaac aaaaatata
//
LOCUS       DAVBHS010000028        17538 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVBHS010000028 DAVBHS010000000
VERSION     DAVBHS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17538
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.028"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            16..210
                     /locus_tag="VQ651_000680"
     CDS             16..210
                     /locus_tag="VQ651_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET2753705.1"
                     /translation="MGAEAIRRNCERGAIFSGKIKHHEDSQFKGDHYVECYAVLDNTV
                     IARDRITVPIDPLCGKDFIE"
     gene            554..1888
                     /gene="trmFO"
                     /locus_tag="VQ651_000681"
     CDS             554..1888
                     /gene="trmFO"
                     /locus_tag="VQ651_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET2753706.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRIFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEAIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            1972..2709
                     /locus_tag="VQ651_000682"
     CDS             1972..2709
                     /locus_tag="VQ651_000682"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET2753707.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            2718..3275
                     /gene="frr"
                     /locus_tag="VQ651_000683"
     CDS             2718..3275
                     /gene="frr"
                     /locus_tag="VQ651_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET2753708.1"
                     /translation="MANAIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRIHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVV
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3335..4189
                     /gene="cvfB"
                     /locus_tag="VQ651_000684"
     CDS             3335..4189
                     /gene="cvfB"
                     /locus_tag="VQ651_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HET2753709.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTSLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4198..4413
                     /locus_tag="VQ651_000685"
     CDS             4198..4413
                     /locus_tag="VQ651_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HET2753710.1"
                     /translation="MRKSFYTWLMTERNPKSNNPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4499..5467
                     /locus_tag="VQ651_000686"
     CDS             4499..5467
                     /locus_tag="VQ651_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET2753711.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMALVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            5647..6147
                     /locus_tag="VQ651_000687"
     CDS             5647..6147
                     /locus_tag="VQ651_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2753712.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            6150..6410
                     /locus_tag="VQ651_000688"
                     /pseudo
     CDS             6150..6410
                     /locus_tag="VQ651_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(6777..7888)
                     /gene="ald"
                     /locus_tag="VQ651_000689"
                     /pseudo
     CDS             complement(6777..7888)
                     /gene="ald"
                     /locus_tag="VQ651_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253293.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            8065..8634
                     /locus_tag="VQ651_000690"
     CDS             8065..8634
                     /locus_tag="VQ651_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2753713.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            8702..9352
                     /locus_tag="VQ651_000691"
     CDS             8702..9352
                     /locus_tag="VQ651_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET2753714.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTSVKETNLQ
                     AEVAAVSKDSSTGKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            9336..11576
                     /locus_tag="VQ651_000692"
     CDS             9336..11576
                     /locus_tag="VQ651_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HET2753715.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGVFGFWFLFQTWQQSQVSQNLVDSVERVRILPDTIKVNGDSL
                     SFRGKSDGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFDYQGY
                     LKTQGIYQTLNIKRIQSLQKVGSWDIGENLSSLRRKAVVWIKMHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     VFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVASRPLVFGQPNEWLLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIKKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            11756..11944
                     /locus_tag="VQ651_000693"
     CDS             11756..11944
                     /locus_tag="VQ651_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753716.1"
                     /translation="MTVTKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGGY"
     gene            11976..12563
                     /locus_tag="VQ651_000694"
     CDS             11976..12563
                     /locus_tag="VQ651_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2753717.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            12567..13742
                     /locus_tag="VQ651_000695"
     CDS             12567..13742
                     /locus_tag="VQ651_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753718.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWKDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKRIF"
     misc_feature    13916..14047
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14063..14593
                     /gene="infC"
                     /locus_tag="VQ651_000696"
     CDS             14063..14593
                     /gene="infC"
                     /locus_tag="VQ651_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET2753719.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            14626..14826
                     /gene="rpmI"
                     /locus_tag="VQ651_000697"
     CDS             14626..14826
                     /gene="rpmI"
                     /locus_tag="VQ651_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET2753720.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            14878..15237
                     /gene="rplT"
                     /locus_tag="VQ651_000698"
     CDS             14878..15237
                     /gene="rplT"
                     /locus_tag="VQ651_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET2753721.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15295..15675
                     /locus_tag="VQ651_000699"
     CDS             15295..15675
                     /locus_tag="VQ651_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2753722.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            15925..16725
                     /locus_tag="VQ651_000700"
     CDS             15925..16725
                     /locus_tag="VQ651_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HET2753723.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHESGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVNQRVCEDGPVFRTGTVVL"
     gene            16688..>17538
                     /locus_tag="VQ651_000701"
     CDS             16688..>17538
                     /locus_tag="VQ651_000701"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET2753724.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASA"
BASE COUNT         5246 a         3288 c         3983 g         5021 t
ORIGIN      
        1 tggaaagtaa gaaatattgg agctgaggcg attagaagaa attgtgagcg tggtgctatt
       61 ttttcaggta aaataaaaca tcacgaagat tctcagttta aaggagacca ctatgttgaa
      121 tgttatgctg ttttagataa tacggttata gcaagagata gaataacagt ccctatcgat
      181 ccgttatgtg gaaaagattt tatagagtag catataattg attcttaact ggaatactct
      241 ctatctcttt acatcaagaa aatgactaaa cagggaagtt tgccttcttc cctttttttg
      301 ttatactagt agaagaaaaa aatagaaaga tttgtgggag tgaaaaagtc cgggggactt
      361 tttcagcttg agccaagaaa ttcgagagcg aggaaagtcc gggggacttt ttcagcctga
      421 gccaagaaat tcgagagcga agtaagtcca atggactttt tcagcctgag ccaagaaact
      481 cgaaagctcg taagtctgat tggctttttc aatgtgaatc ttaacttcac actcccaaag
      541 aggtattagt gtcgtgtctc aatcttatat caatgttatc ggtgctggtt tggcaggttc
      601 tgaagcagct taccaaatcg cagagcgtgg tattccagtt aaactatatg aaatgcgtgg
      661 tgtcaagtcg actccccagc ataaaacaga caattttgct gagttggttt gttccaattc
      721 tttgcgtggg gatgctttga caaatgcagt tggtcttctc aaggaagaaa tgcgtcgctt
      781 gggttctgtt atcttggaat ctgctgaggc tacacgtgtt cctgcaggtg gtgcccttgc
      841 agtggaccgt gatggtttct ctcaaatggt gaccgaaaaa gttgccaacc accccttgat
      901 tgaagtggtt cgtgatgaaa ttacagaatt gccgacagat gttattacgg ttatcgctac
      961 tggtcctttg acaagtgatg ccttggctga aaagattcat gcccttaatg acggtgatgg
     1021 tttttatttc tacgatgcgg cagcgcctat tatcgatgtc aacactatcg atatgagcaa
     1081 ggtctacctc aaatcacgtt atgataaggg agaagcggcc tacctcaatg cccctatgac
     1141 caagcaagaa tttatggatt tccatgaagc tttggtcaat gcagaagaag caccgcttaa
     1201 ttcttttgaa aaagaaaagt actttgaagg ctgtatgcct atcgaagtca tggccaaacg
     1261 tggcattaaa actatgcttt atggccctat gaagccagtc ggtcttgagt acccagacga
     1321 ctatacagga cctcgtgatg gagaatttaa aacaccttat gcggttgtgc aacttcgtca
     1381 ggacaatgca gctggtagcc tctacaatat tgttggtttc cagacccacc tcaaatgggg
     1441 agaacaaaag cgtatcttcc aaatgattcc gggtcttgaa aatgcagagt ttgtccgtta
     1501 tggtgtcatg catcgcaatt cttacatgga ttcaccaaat cttcttgagc agacttaccg
     1561 ttctaagaaa caaccaaatc tcttctttgc tggtcagatg acgggggtgg aaggctatgt
     1621 tgagtcggcg gcttcaggct tagttgcggg aattaacgca gctcgtctct tcaaggaaga
     1681 aagcgaggct attttccccg agacgacagc gattggaagc ttagctcatt acattaccca
     1741 tgccgacagc aaacatttcc aaccaatgaa tgtcaatttt gggatcatca aggagttgga
     1801 aggcgagcgt atccgtgata agaaggctcg ttatgaaaaa attgcagagc gtgcccttgc
     1861 cgacttagag gaatttttga ctgtctaatt tttttgaaag aattgctcat gatactataa
     1921 aaatcttaga aattgtgata aaataggtag gatgaaagaa ggagagtgaa aatggcgaat
     1981 cccaagtata aacgtatttt aatcaagtta tcaggtgaag cccttgccgg tgaacgtggc
     2041 gtagggattg atatccaaac agttcaaaca atcgcaaaag agattcaaga agttcatagc
     2101 ttaggtatcg aaattgccct tgttattggt ggaggaaatc tctggcgtgg agaacctgca
     2161 gcagaagcag gtatggaccg tgttcaggca gattacactg gaatgcttgg gactgttatg
     2221 aatgctcttg tgatggcaga ttcattgcaa caagttgggg ttgatacgcg tgtacaaaca
     2281 gctattgcta tgcaacaagt ggcagagcct tatgtccgtg gacgtgccct tcgtcacctt
     2341 gaaaaaggcc gtatcgttat ctttggtgct ggaattggtt caccatactt ctcgacagat
     2401 acaacagcgg cccttcgtgc agctgaaatc gaagcggatg ccatcctcat ggctaaaaat
     2461 ggtgtcgatg gtgtttacaa tgccgatcct aagaaagata agacagctgt taagtttgaa
     2521 gaattgaccc accgtgacgt tatcaataaa ggtcttcgta tcatggactc aacagcttca
     2581 accctctcaa tggacaacga cattgacttg gttgtcttca acatgaacca atcaggcaac
     2641 atcaaacgtg tcgtatttgg tgaaaatatc ggaacaacag tttcaaataa tatcgaagaa
     2701 aaggaataag aaagaatatg gctaacgcaa ttattgaaaa agctaaagag agaatgaccc
     2761 agtctcacca atcacttgct cgtgaatttg gtggtatccg tgctggtcgt gccaatgcaa
     2821 gcttgcttga ccgtatacat gtagaatact atggagttga aactcctctt aaccaaatcg
     2881 cttcaattac gattccagaa gcgcgtgttt tgttggtaac accatttgac aagtcttcat
     2941 tgaaagacat cgaacgtgcc ttgaacgctt ctgatcttgg tatcacacca gctaatgacg
     3001 gttctgtgat tcgcttggtt gtcccagctc ttacagaaga gactcgtcgt gaccttgcta
     3061 aagaagtgaa gaaggtcggc gaaaatgcta aagtggctgt ccgcaatatc cgtcgcgatg
     3121 ctatggacga agctaagaaa caagaaaaag caaaagaaat cactgaagac gaattgaaga
     3181 ctcttgaaaa agacattcaa aaagtaacag acgatgctgt taaacacatc gacgatatga
     3241 ctgccaacaa agagaaagaa cttttggaag tctaaaaata aacagaaaaa ctcagttggc
     3301 attgctggct gagttttatt cgaaagaagg aaatatgaat acaaatcttg caagttttat
     3361 cgttggactg atcatcgatg aaaacgaccg tttttacttt gtgcaaaagg atggtcaaac
     3421 ctatgctctt gctaaggaag aaggccaaca tacagtaggg gatacggtca aaggttttgc
     3481 atacacggat atgaagcaaa aactccgcct gacaagctta gaagtgactg ccactcagga
     3541 ccaatttggt tggggacgtg tcacagaggt tcgtaaggac ttgggtgtct ttgtggatac
     3601 aggccttcct gacaaggaaa tcgttgtgtc actcgatatt ctccctgagc tcaaggaact
     3661 ctggcctaag aagggcgacc aactctacat ccgtcttgaa gtggataaga aagaccgtat
     3721 ctggggtctc ttggcttatc aagaagactt ccaacgtctt gctcgtcctg cctacaacaa
     3781 catgcagaac caaaactggc cagccattgt ttaccgtctc aagctatcag gaacttttgt
     3841 ttacctacca gaaaataata tgcttggttt tattcatcct agcgagcgtt acgcagagcc
     3901 acgtttgggg caagtattag atgcgcgcgt tattggtttc cgtgaagtgg accgcacttt
     3961 gaacctctcc ctcaaaccac gctcctttga aatgttggaa aacgatgctc agatgatttt
     4021 gacttatttg gaaagcaatg gcggtttcat gaccttaaat gacaagtcat ctccagacga
     4081 catcaaggca acctttggca tttctaaagg tcagttcaag aaagctttag gtggtcttat
     4141 gaaggctggt aaaatcaagc aggaccagtt tggtacagag ttgatttagg gaggcttatg
     4201 agaaaatcat tttacacttg gctcatgacc gagcgcaatc ctaaaagtaa caatcccaaa
     4261 gcaattttgg cagacctcgc ttttgaagag gcagcttttc caaaacatac agatgatttt
     4321 gatgaggtca gtcgcttttt ggaggagcat gccagtttct cttttaacct aggagatttt
     4381 gacagcattt ggcaggaata tctagaacac tagcatttat tcattgggtt tgggctagta
     4441 atttctccat ccctctgcta taataaaaag aaataaaagg attagagagg ttctttattt
     4501 gaaggaacat tcaatagaca ttcaactgag tcatccagat gacctgtttc atctttttgg
     4561 ttccaatgaa cgccatcttc gtttgatgga agaagagctt gatgttgtga ttcatgctcg
     4621 tacggagatt gtccaggttt tgggagaaga gtctgcttgc gaggaagccc gtcaggttat
     4681 tcaagctctc atggccttgg taaatcgtgg gatgaccgtt ggtacgccag atgtagtgac
     4741 ggctattagt atggtcaaaa atgatgaaat tgacaagttt gtcgcccttt acgaagaaga
     4801 aatcatcaag gataatactg ggaagcctat ccgcgtcaaa accttaggtc aaaaacttta
     4861 tgtggacagt gtcaaacagc atgatgtgac ctttggaatt gggccagcag gtacagggaa
     4921 gaccttcctt gcagtgacct tggcagtgac tgcccttaaa cgtgggcaag tcaagcgaat
     4981 tatcctaact cgtccagcgg tggaagcggg agagagtctt ggatttcttc cgggtgatct
     5041 taaggagaag gtggatcctt accttcgtcc tgtttacgat gccttgtatc aaattcttgg
     5101 gaaagaccaa acgactcgtc tcatggagcg tgaaattatc gaaattgcgc cccttgccta
     5161 tatgcgtggc cggaccttgg atgatgcctt tgtcattctc gatgaggcgc aaaacacgac
     5221 catcatgcag atgaagatgt tcttgacgcg tttaggcttt cattctaaga tgattgtcaa
     5281 tggagatatc agtcagattg acctgccacg taatgtcaag tccggtttga ttgatgctca
     5341 agagaaactc aagaacatcc atcagattga ctttgttcat ttttcagcca aggatgtggt
     5401 tcgccatcca gttgtcgctc agattatccg agcctatgaa tattctactg aagttgcaca
     5461 cgactgattt tgaggaagtt cgcctgcaaa agaatatact tttgagagag gaaaatccag
     5521 ttgtataggc taaaggtttg agacaacgat taggcacgat ggaaagaact tttatgtggc
     5581 tgatgacgat cagtgcatct tcctgtgtca taatcacagg gcacaagaaa gtaggaattt
     5641 gaaaagatga ttgaccaact atctaagtat tacagttgta ggatactaac tgaaaaggat
     5701 attccaagta ttttatcttt atatgaaagt aatcctctgt attttcagca ttgtccacca
     5761 gagccaaatt ttgcaactgt aaaagaggac atgctttgtc tacctgaagg taaagctaag
     5821 gctgataagt tttttgttgg attttggaat ggctctgacc ttgtggctgt tatggatttt
     5881 gtctatgcat atcctgatga ggagactgtt tttattggtt tgtttatggt tgatcaagcc
     5941 tatcagagga aagggattgg tagtcatatt gtgacagaag cactagctta ttttgctaag
     6001 aactttcgaa aggcacgttt ggcttatgtt aagggaaatc cgcaatctca gcatttttgg
     6061 gaaaagcagg gctttaaatc aattggatgc gaggttaagc aagaactcta tacggttgtt
     6121 atcgctgaac agagcctaga agattagaaa tggcatcaag taagaactat ttggaatttg
     6181 ttttggaaca attatcagga ttagatgatg tgacttaccg ttccatgatt ggggagtata
     6241 ttctttactt ccgcggcaag attattggcg gcatttatga cgatcgcttt ttagttaaac
     6301 ccgtgcaagc agtcttagat aagattgacc aatcttcttt tgagtttcca tacaaaggtg
     6361 ccaaagaaat gatttgagtg gaagaacttg ataataagat gtttctataa gacctgattt
     6421 tagctatgta taaccaactg ccaacgccca aacctaaaaa gaaaaagcaa gggtgaacga
     6481 agtaaaaaag aagtctgcta aggccctgtc tttgcacggg taaaatttta tatataaaaa
     6541 gaagctggga ctaaagagct cagcttcctt tggtttatat aattgtcatt acaagacgaa
     6601 gtggttgggc gaaactctgt tgactttatt caatttagag tttcttatgc acaattgagt
     6661 ctggaacgaa agtctccagt tgcaaagtat acagtacaat aaaccaacga tgtaatagct
     6721 gatgacacaa agcacagtgg gtaggacttg cgaagtcacc cttttctttt caaaatttat
     6781 actaaatcat tgatatcagt gtagtcacga ttaagtcctt gagcaactgg taggctagtc
     6841 aagtaacctt gataagtggt cacaccttga cgcaagcctt catcttcaga gattgcttgt
     6901 gcgaatcctt tgccagccaa agcttcgata taaggaagag tgacattggt tagggcgatg
     6961 gttgaagtgc gggcaaccgc accagggata ttggcaacgg catagtggag aacaccgtgt
     7021 ttttcataga cgggttcatc gtgcgttgtc acacggtcag ctgtttcgat aacgccacct
     7081 tggtcaacag caacgtcaac gatacagagc ctggacgcat ttgtttgacc atctcatctg
     7141 tcaccaattc cggtgctttt gcaccaggga tgagaatggc tccaatcacc acatcagcat
     7201 ctctcacact tgcttcaatg ttgaatgaat tagatataat agtttgaatt tgacttccaa
     7261 agacttcttc tagaactgag agacgcttgg cactaatatc taaaatagtc acttgagcac
     7321 caagaccaag ggcgatgcgg gcagcatgtg taccgacgac accatcaccg atgatagtta
     7381 cttttccttt tggaacacct ggtacaccac caagtagaac accagagcca ccagcttgct
     7441 tagtaaggaa gtgagctccg atttgaacag ccatacgacc tgcaacctca ctcataggaa
     7501 cgaggagcgg tagttgtcct tgattgtcac gaacagtttc ataggcaatt cctgttgttt
     7561 ttgctgctaa catagcatct gctaattctg gagcagcggc catgtgcaag taggtgaaga
     7621 gaagaagatc gtcgcgcaag taaccgtatt cagaacttaa aggttctttt actttcacaa
     7681 ccaactctgc tgcccaagct tcaccagcag tagcgacaat ctcagctcct tgcttttgat
     7741 agtcagcatc agtaaagcca gaaccgagac cagcatttgt ttcgataagg acacgatgac
     7801 cacgactaac taagctatga acacctgcag gtgtgagggc gacacggttt tcgttatttt
     7861 taatttcttt tgggattccg attaacattg agataaccta cctttcaatt gacggtcttg
     7921 ttttggttgt cacattccag ttcataaatc aaaaatgtga cggtttcatt gtatatgaaa
     7981 ccgcttcaaa aatcaagaaa aacttgtcat ccaaattttt ttatgctaga ctagtgaaaa
     8041 tcaagctcta atggagggaa aagtatggaa tcaatatttg tgaaatttgc ccagtatccg
     8101 tctatagaaa cggagcgttt attgcttaga cctgtaactt tggatgatgc ggaagcaatg
     8161 tttgactatg cctcggacaa gggtaataca cgttacactt ttccaaccaa tcaaagcttg
     8221 gaagaaacca agaataacat tgctcagttc tacttggcta atcccttggg acgttggggg
     8281 attgaattaa aagataacgg caagtttatc ggaactattg acttacacaa gattgatcct
     8341 gttcttaaga aggcagctat tggctacatt atcaataaaa agtattggaa tcaaggatta
     8401 acgacagaag ccaatcgtgc tgtgattgag ctagcttttg agaagatagg gatgaataag
     8461 ttgactgccc ttcacgataa ggctaatccc gcgtcaggaa aggtcatgga gaaatcaggc
     8521 atgcgttttt cccatgcaga accatatgct tgtatggacc agcatgaaaa aggccgaatc
     8581 gtgacaagag ttcattatgt cttgaccaag gaagactatt ttgcaaataa ataagcagtt
     8641 gaaaagaaat ttttcgactg ttttttcttc ctcttacgaa taatctaaga gaggagaaaa
     8701 tatggaagca attatcgaga aaatcaaaga gtataaaatc atcgtcatct gtactggtct
     8761 gggcttgctt gtaggaggat ttttcctgct aaagccagct ccacaaacat ctgtcaaaga
     8821 gacgaatttg caggctgaag ttgcagctgt ttccaaggac tcatcgaccg gaaaggaagt
     8881 gaacaaggaa gagaaggaag aaccagttga acaagatcta atcacagtag atgtcaaagg
     8941 tgctgtcaaa tcgccaggga tttatgactt gcctgtaggt agtcgagtca atgatgctgt
     9001 tcagaaggct ggtggcttga cagagcaagc agacagcaag tcgctcaatc tagctcagaa
     9061 agttagtgat gaggctctgg tttacgttcc tactaaggga gaagaagcag ttagtcaaca
     9121 gactggttcg gggacagctt cttcaacaag caaggaaaag aaggtcaatc tcaacaaggc
     9181 cagtctggaa gaactcaagc aggtcaaggg actgggagga aaacgagctc aggacattat
     9241 tgaccatcgt gaggcaaatg gcaagttcaa gtcagtagac gagctcaaga aggtctctgg
     9301 cattggtgcc aagaccatag aaaaacttaa agactatgtt acagtggatt aagaatttct
     9361 ctattcccct aatttacctg agttttctat tactatggct ttactacgcc attttctcag
     9421 tatcctattt tgctttgttg ggttttgttt ttctgctagt ctgcctcttt atccaatttc
     9481 cttggaaatc agcaggtaaa gttctagtga tttgtggagt ctttggcttc tggtttctgt
     9541 ttcaaacttg gcaacagagt caagtgagtc aaaatttggt ggattctgtt gaaagggtac
     9601 ggattttacc agacactatt aaggttaacg gtgacagtct gtcctttcgt ggtaagtctg
     9661 atggtcgtgc tttccaagtc tattataaac tccagtccga ggaggagaaa gaagcctttc
     9721 aagctttaac tgaccttcat gagataggac tagaagggaa gctttcggag ccagaagggc
     9781 agagaaattt tggtggcttt gactaccaag gctatctgaa gactcaggga atttaccaga
     9841 cactcaatat taaaagaatc cagtcactcc aaaaggttgg cagttgggat ataggtgaaa
     9901 acctgtccag tttacgtcga aaggctgtgg tttggattaa gatgcacttc ccagacccta
     9961 tgcgcaatta catgacagga ctcttgctag gacatctgga caccgatttt gaggagatga
    10021 atgagcttta ttccagttta ggaattattc acctttttgc cttgtcaggt atgcaggtag
    10081 ggtttttcat ggacggattt aagaaactac ttttacgatt gggcttgaca caagaaaagt
    10141 tgaagtggct gacttatccc ttttccctta tctatgcggg actaactgga ttttcagcat
    10201 cggttattcg cagtctcttg caaaagctac tggctcaaca tggggttaag ggcttggata
    10261 attttgcctt gacggtgctt gtcctcttta ttgtcatgcc aaactttttc ttgacagcag
    10321 gaggagtctt gtcctgtgct tatgctttta tcctgaccat gaccagcaaa gaaggggagg
    10381 ggctcaaggc tgttactagt gaaagtctag tcatctcctt gggcatattg cccattctat
    10441 ccttctattt tgcggaattt caaccttggt ctatcctttt gacctttgtc ttttcctttc
    10501 tttttgactt ggtcttctta ccgctcttgt ctatcttatt tgtcctttcc tttctctatc
    10561 cagtcattca gctgaacttt atctttgaat ggttagaggg cattattcgc ttggtctcgc
    10621 aggtggcaag tagaccgctt gtctttggac aacccaatga atggctttta atcctattgt
    10681 taatttcttt ggctttggtc tatgatttga ggaaaaacat taaaaagcta acggtattgt
    10741 gcttattgat tacagggctc tttctcctga ccaagcatcc actggaaaat gaaatcacca
    10801 tgctggatgt ggggcaagga gaaagtattt tcctacggga tgtaactggg aaaaccattc
    10861 tcatagatgt aggtggtaag gcagaatctt ataagaaaat caaaaaatgg caagaaaaga
    10921 tgacgaccag caatgcccag cgaagcttga taccctatct caaaagtcga ggagtagcta
    10981 agattgacca gctaattttg actaacacgg acaaggagca tgttggagat ttgtcagaga
    11041 tgaccaaggc tttccatgta ggggagattc tagtatcaaa agacagtctg aaacagaagg
    11101 aatttgtggc agaactacag gcgactcaaa caaaggtgcg tagtatgata gtaggggaga
    11161 acttacccat ttttggaagt cagttagaag ttctatctcc aaggaaaatg ggagatggag
    11221 gacacgatga taccctagtt ctgtatggga aattcttgga taagcaattt ctcttcacgg
    11281 gaaatttgga ggagaaagga gagaaggact tgctgaagca ctatccagac ttgaaagtaa
    11341 atgttttgaa agctagccaa catggcaata aaaaatcatc aagtccagcc tttctagaaa
    11401 aactcaaacc agagcttact cttatctcag ttggaaagag caatcgaatg aaactccccc
    11461 atcaggaaac attgacacga ctggaaggta tcaatagcaa agtttatcga actgaccagc
    11521 aaggagctat acgttttaag gggttggata gttggaaaat cgaaagtgtt cgataggaag
    11581 gataaatgtt gtagattagt gaaataaact aaaaatttgt tgcataataa tgataaaaac
    11641 ggcataatga aaacgtattc aatattgagg atataaaatc attaaaaatc agcaaaagtt
    11701 gttttagtta gttagtttat aatctattgg tcttcttcag tccagtgtat ctgctgtgac
    11761 agtcactaaa agttacaagt atgattggaa tacggttttg gaatatagta ccaactatca
    11821 cgaccatcag tatgcttgga ttccggaatg gtctcgttat tacagctatt ctgagtataa
    11881 agttggcgga ggctggaact acgctcgtta tgaggtcata aactactata ctggaggtta
    11941 ttaatcctta aagagtgaga aaaggagggc tggatatgtt aaatcttact catgttacct
    12001 taaaaacgcg acaagtcatc ttgcaagatg cggattttac ctttaaaaag ggtaggattt
    12061 atggccttct tgctatcaat ggctcgggaa agacgacact attccgagct atgagcaagt
    12121 tgcttcccct tagtagtgga cacatcgcag ttcctccttc tttgttttat tatgagagcg
    12181 ttgaatggct ggatggaaac ttaagtggga tggactacct tcgtctcata aaaaacatct
    12241 ggaagtcaga cctaaacttg agagatgaaa tcgcctactg ggaaatggct gactatatca
    12301 gtcttcccat ccgcaagtat tccttaggga tgaagcaacg cttggtgatt gctatgtatt
    12361 ttctcagtca ggccaaatgc tggctcatgg atgagattac aaatggctta gacgagtatt
    12421 atcgacagaa gttttttgat aggctagcac aaatcgatag acaagaacag ctggttcttt
    12481 taagttccca ctataaggaa gagttggttg atatctgcga tagagtagta accattcatc
    12541 aggggcagat agaagaggtt tagtttatga aagatgttag tctattttta ttgaaaaaag
    12601 ttttcaaaag tcgtttaaac tggattatct tagctttatt tgtatctgta ctcggtgtta
    12661 ccttttattt aaatagtcag actgcaaact cacacagctt ggagagcagg ttggaaagtc
    12721 gcattgcagc caacgagagg gctatcaatg aaaatgaaga gaaactctcc caaatgtctg
    12781 ataccagctc ggaggaatac cagtttgcta aaaataattt agacgtgcaa aaaaatcttt
    12841 tgacgcgaaa gacagaaatt ctaactttac taaaagaagg gcgctggaaa gaagcctact
    12901 atctgcagtg gaaagatgaa gagaagaatt atgaatttgt atcaaatgac ccgactgcta
    12961 gctctggctt aaaaatgggg gttgaccgcg aacggaagat ttaccaagcc ctgtatccct
    13021 tgaacataaa agcacatact ttggagtttc cgacccacgg gattgatcag attgtctgga
    13081 ttttagaggt tatcatccca agcttgtttg tggttgcgat tatttttatg ctaacacaac
    13141 tatttgcaga aagatatcaa aatcatctgg acacagctca cttatatcct gtttcaaaag
    13201 tgacatttgc aatatcctct cttggagttg gagtgggata tgtaactgtg ctgtttatcg
    13261 gaatctgtgg cttttctttt ctagtgggaa gtctgataag tggttttgga cagttagatt
    13321 atccctaccc aatttatagc ttagtgaatc aagaagtaac tattgggaaa atacaagatg
    13381 tattatttcc tggcttgctc ttagctttct tagcctttat cgtcattgtg gaagttgtgt
    13441 acttgattgc ttactttttc aagcaaaaaa tgcctgtcct ctttctttca ctcattggga
    13501 ttgttggctt attgtttggc atccaaacaa ttcagcctct tcaaaggatt gcacatctga
    13561 ttccctttac ttacttgcgt tcagtggaga ttttatctgg aagattacct aagcagattg
    13621 ataatgtcga tctaaattgg agcatgggaa tggtcttact tccttgcctg attatctttt
    13681 tgctattggg aattctattt attgaaagat ggggaagttc acagaaaaaa agaatttttt
    13741 aatagattct agctttccta taggtaggga aaataagtaa aaactaacat agagagggaa
    13801 tcaacttgat tctctctttt tgattcgaaa accaaaccaa aatacaaaca caaacttttc
    13861 aaaaaataac tttttatctt gacaagagct agaaaacttg gtatcatata aaagttgaga
    13921 aaagcagaag tgagagcttc tcgccttgtg acattaagtt gcctggccct acggatgaaa
    13981 agtttcgaag aaacgctatc ataacgtgcg ggcttgtata tttacgagtc cgctattgtt
    14041 tttctctaat aaaacaaaag aggtgaaaac catagcaaag caagacttat tcatcaatga
    14101 tgagattcgt gtacgtgaag ttcgcttgat tggtcttgaa ggagaacagc taggcatcaa
    14161 gccactcagt gaagcgcaag ctttggctga taacgctaat gttgacctag tattgattca
    14221 accccaagcc aaaccgcctg ttgcaaaaat tatggactac ggtaagttca aatttgagta
    14281 ccagaagaag caaaaagaac aacgtaaaaa acaaagcgtt gttactgtga aagaagttcg
    14341 tctaagtcca actattgaca agggtgactt tgatacaaaa cttcgtaatg cacgtaagtt
    14401 ccttgaaaaa ggaaataaag ttaaggtatc tattcgcttt aagggtcgta tgattaccca
    14461 taaagagatt ggtgcaaaag ttttagccga gtttgctgaa gcaactcaag atattgccat
    14521 catcgaacaa cgtgctaaaa tggatggacg tcaaatgttc atgcaattgg cgccagcgac
    14581 tgacaaaaaa taattgtcag aaagttaaat aaaggagaaa aaattatgcc aaaacaaaaa
    14641 acacaccgcg catcagctaa acgtttcaaa cgtacaggtt ctggtggact taaacgtttc
    14701 cgtgcttaca cttctcaccg tttccacgga aaaactaaga aacaacgtcg tcatcttcgt
    14761 aaagcatcta tggtgcattc aggagattac aaacgtatca aagcaatgct tactcgcttg
    14821 aaataatagc ttgactgtaa gtaaacaatt ctaggaaata tttggaggaa ataaaacatg
    14881 gcacgtgtta aaggtggcgt tgtatcacgc aaacgtcgta aacgtattct taaattagca
    14941 aaaggttact atggagctaa acacatcttg ttccgtactg caaaagaaca agtaatgaac
    15001 tcttactact atgcataccg tgaccgtcgt cagaaaaaac gtgacttccg caaattgtgg
    15061 atcactcgta tcaatgcggc agctcgtatg aacggacttt catactcaca attgatgcat
    15121 ggtttgaaat tggctgagat cgaagttaac cgtaaaatgc ttgctgactt ggctgttaac
    15181 gatgcagtag ctttcacagc tcttgcagat gcagctaaag caaaacttgg taaataacaa
    15241 ctgatgagac tggaacagga ttgtccagtc tttttaaaaa taaaggagaa aaatatggct
    15301 tcaaaaatgc tacacacttg cttacgagta gaaaatcttg aaaaatcaat cgcattttat
    15361 caagatgctt ttggttttaa agaattgcgt cgcagagatt ttccagacca tgccttcacg
    15421 attgtctatc taggtcttga gggtgatgac tatgagttgg agttgactta taactacgat
    15481 cacggtccgt atgtggttgg tgatgggttt gcccatatcg ccctcagtac acctgatctt
    15541 gaggcacttc atcaagaaca cagtgcaaaa ggctatgaag tgactgagcc aaatggtcta
    15601 ccaggaacta caccaaacta ttactttgtc aaagaccctg atggatataa ggtcgaagtc
    15661 attcgtgaaa aataatttcg tgcaagttaa gtagaatatt cgttttttac ttgacttttt
    15721 ttagctgaaa gagtatacta ataaaaattc aacctttaag gggagtccag agagactcac
    15781 aaggtgtcag ataaaagaat agtgcaattt tctagaggag actttttgag tgtgctctct
    15841 tgtgttgtac gattttaact gaggccttgc actagcaagg tcttttcttt atctggtccc
    15901 cttaaaattt aaggaggaaa agttatgaat cccacatgta agaagcgttt gggtgtcatt
    15961 cggttggaaa ccatgaaggt ggttgcacaa gaggaaatcg cgccagcaat ctttgaatta
    16021 gtcctagaag gagaaatggt tgaagccatg cgagcaggcc aatttcttca tctgcgtgta
    16081 ccagacgatg cccatctttt acgtcgtcct atttcaattt cgtctattga caaggcaaac
    16141 aagcagtgtc acctcattta tcggattgag ggagctggga ctgcaatttt ttcaacctta
    16201 agtcagggag acactcttga tgtgatgggg cctcagggaa atggttttga cttgtctgac
    16261 cttgatgagc agaatcaggt tctccttgtt ggtggtggga ttggtgttcc acccttgctt
    16321 gaggtggcca aggaattgca tgaaagtgga gtgaaagtag tgacagtcct cggttttgct
    16381 aataaggatg ctgttatttt gaaaacggaa ttggctcagt atggtcaggt ctttgtaacg
    16441 acagatgatg gttcttatgg catcaaggga aatgtttccg ttgttatcaa tgatttagat
    16501 agtcagtttg atgctgttta ctcgtgtggg gctccaggaa tgatgaagta tatcaatcaa
    16561 acctttgatg atcacccaag agcctattta tctctggaat ctcgtatggc ttgtgggatg
    16621 ggagcttgct atgcctgtgt tctaaaagta ccagaaagcg agacggtcaa ccaacgcgtc
    16681 tgtgaagatg gtcctgtttt ccgcacagga acagttgtat tataaggaga aaattatgac
    16741 tacaaatcga ttacaagtgt ctctacctgg tttggatttg aaaaatccga ttattccagc
    16801 atcaggttgt tttggctttg gacaagagta tgccaagtac tatgatttaa accttttagg
    16861 ttctattatg atcaaggcga caacccttga accacgtttt gggaacccaa ctccaagagt
    16921 agcagagacg cctgctggta tgctcaatgc aattggcttg caaaatcctg gtttagaggt
    16981 tgttttggct gaaaagctac cttggctgga aagagaatat ccaaatcttc ctattattgc
    17041 caatgtagct ggtttttcaa aacaagagta tgcagctgtt tctcatggga tttccaaggc
    17101 aactaatgta aaagctatcg agctcaatat ttcttgtccc aatgttgacc actgtaatca
    17161 tggacttttg attggtcaag atccagattt ggcttatgat gtggtgaaag cagctgtgga
    17221 agcctcagaa gtgccagttt atgtcaaatt aaccccgagt gtgaccgata tcgttactgt
    17281 cgcaaaagct gcagaagatg cgggagcaag tggcttgacc atgatcaata ctctggttgg
    17341 aatgcgcttt gacctcaaaa ccagaaaacc aatcttggcc aatggaacag gtggaatgtc
    17401 aggtccagca gtctttccag tagccctcaa actcatccgc caagtagccc aaacaacaga
    17461 cctgcctatc attggaatgg ggggagtgga ttcggctgaa gctgccctag aaatgtatct
    17521 ggctggggca tctgctat
//
LOCUS       DAVBHS010000029        17282 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVBHS010000029 DAVBHS010000000
VERSION     DAVBHS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17282
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.029"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..692)
                     /locus_tag="VQ651_000702"
     CDS             complement(<1..692)
                     /locus_tag="VQ651_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET2753725.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSYRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDG"
     gene            complement(803..1057)
                     /locus_tag="VQ651_000703"
     CDS             complement(803..1057)
                     /locus_tag="VQ651_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753726.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQV
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(1415..1948)
                     /locus_tag="VQ651_000704"
     CDS             complement(1415..1948)
                     /locus_tag="VQ651_000704"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET2753727.1"
                     /translation="MTIFKEQESEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLECFFKTR"
     gene            complement(1935..2261)
                     /locus_tag="VQ651_000705"
     CDS             complement(1935..2261)
                     /locus_tag="VQ651_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2753728.1"
                     /translation="MAYNTDFKQGALDSIKGGHRHVEAAKVFWCWRQNSLHVGKERRE
                     QRNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIAAHFDCAVPSVWAALKQIKVTL
                     KKDDDL"
     gene            2495..4213
                     /locus_tag="VQ651_000706"
     CDS             2495..4213
                     /locus_tag="VQ651_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET2753729.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPKEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            4324..4671
                     /locus_tag="VQ651_000707"
     CDS             4324..4671
                     /locus_tag="VQ651_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET2753730.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(4949..5785)
                     /locus_tag="VQ651_000708"
     CDS             complement(4949..5785)
                     /locus_tag="VQ651_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2753731.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(5798..6427)
                     /locus_tag="VQ651_000709"
     CDS             complement(5798..6427)
                     /locus_tag="VQ651_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2753732.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLDNLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(6437..7078)
                     /locus_tag="VQ651_000710"
     CDS             complement(6437..7078)
                     /locus_tag="VQ651_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2753733.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(7224..8453)
                     /locus_tag="VQ651_000711"
     CDS             complement(7224..8453)
                     /locus_tag="VQ651_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET2753734.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEESENLLT
                     KWMIARSYQEMDDLDTAYEHYQELIGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(8443..9609)
                     /locus_tag="VQ651_000712"
     CDS             complement(8443..9609)
                     /locus_tag="VQ651_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET2753735.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            9753..10766
                     /locus_tag="VQ651_000713"
     CDS             9753..10766
                     /locus_tag="VQ651_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HET2753736.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(10811..11230)
                     /locus_tag="VQ651_000714"
     CDS             complement(10811..11230)
                     /locus_tag="VQ651_000714"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET2753737.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDQVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(11241..12647)
                     /gene="atpD"
                     /locus_tag="VQ651_000715"
     CDS             complement(11241..12647)
                     /gene="atpD"
                     /locus_tag="VQ651_000715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET2753738.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFAGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(12733..13611)
                     /locus_tag="VQ651_000716"
     CDS             complement(12733..13611)
                     /locus_tag="VQ651_000716"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET2753739.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(13627..15132)
                     /locus_tag="VQ651_000717"
     CDS             complement(13627..15132)
                     /locus_tag="VQ651_000717"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET2753740.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALIHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(15147..15683)
                     /locus_tag="VQ651_000718"
     CDS             complement(15147..15683)
                     /locus_tag="VQ651_000718"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET2753741.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(15683..16177)
                     /gene="atpF"
                     /locus_tag="VQ651_000719"
     CDS             complement(15683..16177)
                     /gene="atpF"
                     /locus_tag="VQ651_000719"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET2753742.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(16191..16907)
                     /gene="atpB"
                     /locus_tag="VQ651_000720"
     CDS             complement(16191..16907)
                     /gene="atpB"
                     /locus_tag="VQ651_000720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET2753743.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(16942..17142)
                     /locus_tag="VQ651_000721"
     CDS             complement(16942..17142)
                     /locus_tag="VQ651_000721"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HET2753744.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
BASE COUNT         5222 a         3786 c         3096 g         5178 t
ORIGIN      
        1 ccgtcagtag tagtcatgct actgagtttt ttatctgtga cattgtagct ggtaccaatc
       61 agttgttttt tattggtaat gtaggttccg tcactttctt tttctccaat tccagtatca
      121 tttttcaatg atagcaaacg cccttgttca tcaacatagc gaactttcac attttctgag
      181 attagttctg ccaattctga ggtttttttc tttttcttga tttcttcggt tattttccct
      241 ttctcttctt cgggaatatt tagttttgga atgatttttt caacaacggt tcgtgatggt
      301 tccacagtat cttggatgac tgaaaagtca gctagaattg ggagattata ctgaacacgg
      361 taactttgag tgtttactcc tactctttca ttattctctg aaaatacttg tacggtataa
      421 gaaacaacat cttttcctaa tagaacatcc ccagtagaga aatagccgcc ttttcctagt
      481 ttgctatctc cagagcccac ttctttccta attttatcag atagtttttt accagtcagt
      541 acattcgttc gcacaatccc tttgtctacc cctacaaagt gggagaactt tttgaactct
      601 tcagaaccag atctagccca accattatta agggcatttg cttttgtatt tgtattctct
      661 ctcaaaggtt tggcgattag aattatattc atcggcactt agagttgctg ctatatctga
      721 ctcttgaata ccaacttcct tactaccatt tctagcggca gtatatgtgt atttatctgt
      781 ttctacttct agctgattat ccttaacatg ataggtaatt ttctcctggt ataagagtct
      841 tcagtagttt atatactctg tcatcatctt tagctttaga agcagctgag tttagccaag
      901 ttggggtatt aggagtatct ttatcaacct gtttcaagac attattcaag tcatttgcta
      961 gcctattggt tatcctcact gtgaaagttt tcttccagtc tgaaccactt ttattagact
     1021 tagatatgct catgaaatcc tggtcaacat tcaacacaat atcgccttgt tttttagcat
     1081 cttccgttgc ttctgcttca aattgatcat ggagtttttg ttgctctggt gtatagtttt
     1141 tttatttaca cgatttaatg tactattttt tattttagta ttaagaactc cattaatacg
     1201 atttttaaac tctgcttcaa aaaatccctt aatttgttgg tgcgcaagtt caacattcaa
     1261 acctttctta tacaagtctt cattctgata caagtcttca ttctgaacga tttcggctac
     1321 acggtgaatg gtttgatcgt ataccgtctt ctcttaacca gcagtatcct tagttttaat
     1381 gactactcga taggttcctt tagaaccgtt atagtcatct agttttaaaa aagcattcca
     1441 aacattcaaa aatagttgga acttccctta ataaatccgt cactttcttt ttcaagatag
     1501 cccaagcttg ctcaatagga ttcaaatccg gtgaataagg tggtagaggt aagaaaaagt
     1561 gtttatcttg gatgcagagt tcatccaaaa tgttcttggg atgaaaacta gcattgccca
     1621 tgacaataac atgaggtgtc ttcaaagcag gtaggagttg cgttttgaac cactccacaa
     1681 agaaatcgct tgtcatgctt ttcttgtaaa tcatgggagc tataaactct ccgtctactt
     1741 gtcctgcaac aattgaagtt cgctcaaaac gacgtccgct aatcttttca tagactttct
     1801 cccctctagg agcccctgca taaggacgat agaggtagcg gtcgattcct gtttcatcaa
     1861 tatatacgat tggtaaatct tttaggttat ccaaaatatc aagaaactca gcgactttct
     1921 ctgactcttg ttccttaaag atcgtcatct ttttttaaag tgaccttaat ctgctttaaa
     1981 gctgcccata ctgaaggaac agcacaatca aaatgtgccg caatttcccg taaaaaagca
     2041 tctggatgag cctctacaaa ggctttcaat tcttctaaag ggatcttacg ctttttgacg
     2101 actcgctttt tccgctctaa gttcctttgt tcacgtcttt cttttcccac gtgaagagag
     2161 ttctgacgcc aacaccaaaa aactttggcc gcctcgacat gtctgtgccc tcctttgatg
     2221 gaatctaatg ctccttgttt aaaatcagta ttatatgcca tagctttatt agaccatgct
     2281 tattttaaac taagcaacta tatcaatgtt ttgttatgag aagacttact ttttttactg
     2341 aaattgaatt tttcctagcc tcaacatcat ataaccccaa aattcaatct gacctggttt
     2401 gaagagatgt tcgaagcaga taaggtttta ctaaaactaa aaaaaatgat ataatcaaac
     2461 tgattaaatg ttttaaaaat aaaggagaat ttgaatgtct taccaagaaa attaccagaa
     2521 atgggttgat tttgtggagc ttcctgacta ccttcgtcaa gatttggaaa atatggacga
     2581 aaaaactaag gaagatgcct tctatacaaa tcttgaattt ggtactgcag gtatgcgtgg
     2641 cttggttggt gctggtacaa accgtatcaa catctacgtt gttcgccaag ctactgaagg
     2701 attggctcgt ttgattgagt caaaaggtgg aaacgagaaa gaacgcggtg tagcaattgc
     2761 ctacgatagc cgtcacttct cacctgagtt tgcctttgaa tctgcggcag ttcttgctaa
     2821 acacggcatc aaatcttacg tatttgaaag ccttcgtccg actccagaac tatcatttgc
     2881 agttcgtcat ctcaactgtt tcgcaggtat catggtcaca gccagccaca accctgctcc
     2941 atttaacggt tacaaggttt acggtgaaga cggtggacaa atgcctccac acgatgcgga
     3001 cgctttgact acttatatcc gtgcaatcga aaacccattt gcagttgaag ttgctgatgt
     3061 ggaaactgaa aaagcttctg gcttgattga agttatcggc gaagctgttg acgtagaata
     3121 ccttaaagag gttaaggacg taaacatcaa cccagccttg attgaagaat ttggtaaaga
     3181 catgaagatt gtctacacac cacttcatgg tactggtgaa atgttggctc gtcgtgctct
     3241 tgcccaagca ggatttgact ctgttcaagt tgttgaagcg caagcaactg ctgacccaga
     3301 cttctcaact gtaacatctc caaacccaga aagccaagca gcctttgccc ttgctgaaga
     3361 acttggtcgt caagttggtg cagatgttct tgtcgcaact gacccagacg ctgaccgtgt
     3421 tggtgttgaa gttcttcaaa aagatggtag ctacctcaac ctttcaggta accaaatcgg
     3481 tgctatcatg gctaaataca tcttggaagc tcacaaaaac gctggaactc ttcctgaaaa
     3541 tgccgctctc tgcaaatcta tcgtttcaac tgacttggta acgaagattg ctgaaagcta
     3601 cggcgcaact atgttcaacg tcttgacagg tttcaaattt atcgctgaaa aaatccaaga
     3661 attcgaagaa aaacacaacc acacttacat gatgggattt gaagaaagct tcggttactt
     3721 gattaaacca tttgtacgtg ataaagatgc catccaagcc gttcttgtcg ttgctgaact
     3781 tgctgcctac taccgttctc gtggtttgac acttgctgac ggtatcgaag aaatctataa
     3841 agaatatggc tactacgcag aaaaaacaat ctctgttact ctttcaggtg tcgatggtgc
     3901 tgaacaaatc aaagcgatta tggctaaatt ccgcaacaat gctccaaaag aatggaacgc
     3961 aacagctatc actgtcgtag aagacttcaa ggcacaaact gctactgttg ctgacggtac
     4021 tgttacaaac ttgacaactc ctccaagtga tgtgttgaaa tacacacttg ctgacggttc
     4081 atggattgcc gttcgccctt caggtacaga accaaaaatc aagttctaca ttgcagttgt
     4141 aggagaaacc aacgaagaat cacaagctaa gattgctaac atcgaagcag aaatcaatgc
     4201 atttgtaaaa taaagaccta taaaagatga gaccaaccaa cctcatcttt tttcgtatca
     4261 aatctaagca attttagaaa ctttatggca tactagactt atcaatgaaa gcgaggtaat
     4321 ctcatggaac aatttttaga taacatcaaa gaccttgaag tcactacagt tgtgcgtgcg
     4381 caagaagctc ttgataaaaa agaaactgca accttcttta tcggtcgcaa aacttgccct
     4441 tactgccgta aatttgcagg tacattgtca ggtgtcgtag ctgaaaccaa agctcacatt
     4501 tacttcatca atagtgaaga accaagccaa ctcaatgatt tgcaagcatt ccgctcacgc
     4561 tatggaatcc caactgtacc aggttttgtt catattacag atggacaaat caacgtccgt
     4621 tgcgactctt caatgtcagc acaagaaatc aaagatttcg caggattgta aaaaatcagc
     4681 taacaagtct taaaatcaca aaaacgcata atgtaaggtg caaaaacctt gatattatgc
     4741 gttttattgt tcctaaaaca gttacatcag catttcatta cagaacattt taattttgga
     4801 agtcaaatca atttttaaca atgttttaga aatcacagtg ggctattcca tattaaatta
     4861 tccagattca atcgctatta tcgttttcct agattacaaa aaaactgcgt tttgctaatc
     4921 aaaacgtagt ctcagccact attttatctt actgtccttc ttttacttct ttggttgcta
     4981 catcttctcc aaaccatttt tggctgattt cttggaactt gccgtccttg taaagactag
     5041 aaaaagcttc atttatcttc ttaaccaagg ttgtatcttc cttacgggct ccaaccgcaa
     5101 aagcttctgt ttctagtcca actgtaaaga cattataatc gtttaaaaca ccttctgctt
     5161 ctaaataata gtttgcatag acacggtcaa tcaatagacc atcaattcga tcgtttttca
     5221 aatcaatcaa ggcttcatta aaggtttggt attgattcgc ttccttatta gcgacaatat
     5281 tcttcaaaat ttctggattt gcttcaaagt ccgcataacc agatgaacca gcttgagctc
     5341 ctaatgtctt tccagtcata tcctttgcag tcgtgatacc agatgatttc ttcgtaacca
     5401 atacctgctc attcttcata tatgagttac tgaaagccac cttttcacgg cgttcgtctg
     5461 tagcggaata gccattccaa atcagatcaa tcgttccttt tgtcaattca gcttctttca
     5521 aatcccaatc aatcggttgc caatttaccg tgattccgta tttttcaaaa acagctgtag
     5581 ctaaatcaat atcaaatcct gcataagaac catctttctg agcaaatccc attggaacaa
     5641 aagtactatc aaatccaata gtaatagact tgttagactg gtactttgac caattatctc
     5701 cactagtttc gctagaattt ttcccacaag ctactaagaa caaagcagtc atcagactga
     5761 ctaatacaag catccatttt ttcatccatt tttcctccta tttaggttct actttcaata
     5821 atacatctgc gatattttca gcaaactgca aatcatgggt aaccacaatc tgggtcatcc
     5881 caagttccct attttgcaag attagcttct ccacttccaa acgtaattct ggatccaggg
     5941 cagaagttgg ttcatcgtag ccaatgattt ctgggtcaat catcatagca cgcgccaaag
     6001 ccacccgctg cttttgccca ccagatagtg agaaaggata ggcctctgcg tgtcctccta
     6061 gtcctaactg ttccaagagt ccactcgcct tcttctcagc ctcttcctgc ttcattccca
     6121 tggtcttcac aggcgataag gtcaaattgt ccagaactga taaatgaggg aacagttgaa
     6181 aatcttggaa tacaaatccc agtagattgc gcttctgcaa ttcatccagc tctaaaggtt
     6241 gtccattata aaagatttgc cctgaatcaa tggtttcaag acctgcaagc atacgtaaaa
     6301 gagttgtctt acctccacca gaaggtccaa cgatagccag gatttgcttt tcaggaatac
     6361 ttagactgaa attagacagg atttgtttgt ctccaaagac tttattgata tttcgtaatt
     6421 ctaacatggc agcctcctat ctataataac tatacttctt ctcaactttt ttggaaataa
     6481 ttgtcacaat cccaatcaaa atcaaataaa tggctcctgc caagaacata ggaactagac
     6541 tagcatcgcg gttagcagct gttcgactag ccaagataag gtctgaaatt ccgagagcat
     6601 agaccaaaga agtatccttg accaaactca taacttcatt aaagacacta ggaagaacga
     6661 tcttggtcac ttggggcaag ataatatagc gcactctgtc aaaagggcta aacttcaaga
     6721 ccttggcggc ctcatactgt cctcttggaa tagtgtcaat tcccccacgg aaaatttctg
     6781 caaagtaagc tgcatagttg agaacaaagg caataatagc tgcaggaagg cggtctaaac
     6841 gaatcccaat acttgggagc acataataga taaaaatcag ttgcaagagt aagggggttc
     6901 ctcgcataac ccagatgtaa atgttaatca gataatggag gggcttccaa tggacttgca
     6961 aggcaaaggc aatcaaaacg cccaagggaa tcgaaaatag caagaccagt gcaaagacct
     7021 gtacagtcgt gctcgcacca ttcagtaaac tcggtaatat ctcaaataaa taagacatac
     7081 tgcacctcct aaaagtaatt gttcctatta tagcatgaat aaacaagatt accaagtatt
     7141 tttgtaaaaa gattctacca tatacaaata aaaaccgtag ctatcaaaag agataaacta
     7201 cagttctata tgtttaaaca ttcttacaat ctctcaaaca gttcttgcat ttgcacatca
     7261 tctggaacca gttttaagta agtgtgagca tggacttttg cttcttcaaa atgtcccaat
     7321 tcacgcaaga gatagatata gtgttccaga aattctggat tgtccttcaa atctcctatc
     7381 aactcttgat aatgctcata agcagtatcc aaatcgtcca tttcttgata agaacgagca
     7441 atcatccact tggtcaaaag attttctgac tcctcactct gcaagtctag aatatcctca
     7501 taacgctcct gctccagata aatagtggct aaacgaagca agatttcttc tgtatcctca
     7561 gcgtcttctt ttgcagtaag gagataattt tctgcaccac tagcatcatg caattcataa
     7621 gaaaattgtg aagcagctag caagaggcga gtttcaaagg gatttttctc taatccttgc
     7681 ttagcgatac gcagggcttc ttgaacttga tgttccttat gtaaagcctg actgtaccca
     7741 tactcatagc cttcaaagtc aggagaaatg gtatcaagct gcttaaagta gagggtggct
     7801 ttttgatatt cttcttgatc aaaataaaga ctggccaact caaaagctgt taagtcatcg
     7861 tattctaact ccagggcttt ttctaaaaac tcagtagccg tttcaaattt ccctaactga
     7921 gcataggcaa agccaattcg ttgataggtg gaaatgcccg tttgctcata aatcgagcga
     7981 ttatctaact gggcataggc ttgaatagcc gcttggtaat tttccaactc actatccaac
     8041 tctgccaaac ccaatatcaa gagagaatcc tctgagtagg tcaaggcctc caataatttc
     8101 tcacgtgcca catctgtcaa accttccagc tggtaaaggt ctgccttcag agccaaagac
     8161 gagacatacc agtcactgtc agcttggatt tcctcaagat aggtaaaggc ttcttctatt
     8221 tgaccatcct cgctagcaat tgcagctaga ttaagatgaa cctctggaaa atcctctaca
     8281 attttcaggt aaatttcctt ggcctgagga tagaaaccaa tcccttcaag ataagttgcc
     8341 aactcataca gaagatcact tgaatcattt tctaaagctt tggcgaaata atgctcagcc
     8401 ttagttaaat cttgctcctc caaagcctgt aacatctgtt gactattgtt cactcttgac
     8461 ttcctcaccc tctaattcat atagaccact gactacctta taccattcga aaatggctga
     8521 aatgacaacc ttagcagagg cataaaccgg aataccaagt aaaactcccc agataccaaa
     8581 catagatcct gaagttaaca aaacaaagag aacattaata gggtggatgt ttaattgact
     8641 tcccaaaatc aatggagaga caaaacggcc ttcaatagtt tgttctacga taaagacaat
     8701 cactactttc aaaagcatga ctggaccagc aatcaaaccc aatactagag caggaagcat
     8761 ggctagaaag ctaccaagat aagggaccag atttaaaata ccagcagtaa cccccagcgt
     8821 aaccgcatag cgtagaccaa taatcttgaa gaagatgata aacattactg ctacaataat
     8881 agccactgtc acttgccctc gaacatagtt ggacaactgt tgattcacat ctgataaaac
     8941 ttgtccaaca ggttccttca attttcttgg aatgaattgg gtcaaatagt tacgcaagcc
     9001 tttcccatca cgcaagagat aaaagagcat gaaaggaacg ataatcaagg caacaatcac
     9061 ttgagaagct ccgctaataa aggcactcac ccagttgact gcctgagatg aaaccttact
     9121 tgccaaaact gtagcctggc tagaaaaatt ggtcaaaact tgctctaatt gaggtctgaa
     9181 atcatctggc aggtgctggg ctaccaatcc attaacaatc ctatctatat cttctaagta
     9241 aacaggaacg tttcttgcaa aggtcaaaac ctgacgttgc agatttggaa tggcgactgc
     9301 caagccccaa atgataaaga gagcgatgat aacaaagaca atagtgatag ctataacacg
     9361 attaacctta tgcttctcca tccaatcaac aataggattc aacaaataat ataacaaacc
     9421 agacaaaatg actggcaaca tcacaactgc taaaaagtct aaaacaggtg aaaatagaaa
     9481 actaatctta cttaaaataa aaagattcag tcccaataat aaggttacta aaaataccgt
     9541 aattgccttg ttatctaaaa accacttgaa aaaccaagat aggctaaaat gtttctcttt
     9601 ttgctccata aatacttcct ttctattgtt ctctcattat accatttttg aaacaaaata
     9661 actctttttt aaagtgctta catctaattg gatatcttta atttacttgt ttttgtgata
     9721 taatagtctt atctttacac agaggtatag taatgaaaga aactgtttat tttggaactt
     9781 atacacgtcg tacttctcaa gggatttaca aggcagactt tgatacagaa actggtcagc
     9841 tttcaaatct agaacttttt gcagctgagc caagtccaac ctaccttgcc tttgaccagc
     9901 accaacattt atacactgtt ggtagccaag acgataaggg gggaattgca gcctatcaaa
     9961 ctgacgggac tgtgttaaat catgttgttg aagaaggagc tccccactgt tatgttgctg
    10021 tcgatgaaaa gcgtgatttg gtttacgcag ccaactatca caagggacaa gtccttgttt
    10081 ataaacgcca ggaagatggt agtcttctac ttagtgatat ggatcaacac agtggccaag
    10141 gtccacatga aaatcaagct tccccccatg ttcactatac agatttaaca cctgaccact
    10201 atctagtgac ctgcgacttg ggaactgacc aagtcatcac ctatgacctc gatcaagaag
    10261 gaaaattatc taagctctat acctatcaca gcaagccagg agcaggctca cgccatatca
    10321 ttttccataa ccactataaa atcgcttatc tcatttgtga actcaatagt actatcgaag
    10381 ttttaatcta cgatggcgtt ggcgaatttg aacgtatgca ggtcatttca actttaccgg
    10441 aagcttacga aggctttaat ggaaccgctg ctattcatct ctctaaagac ggtaaatacc
    10501 tctacgcttc taaccgtggc catgattcta tcgcagtata taccatcctt gcggacggta
    10561 gcttagagtt attagaaatc gttccaacgc atggtcagac tccacgtgat tttgatttga
    10621 cacccgacca aaaatttctc attgttgtcc atcaagactc tgacaatgca actgtcttta
    10681 aacgtaattg tgacaatggt cgtctagcag aactctccaa cgacttccat gttcccgaag
    10741 cagtctgcat ccgttttgct ccttaaaaca ccataaaaaa tgaacttggt attttcaagt
    10801 tcattttttc ttatagtcta tttccgacat taatacggtt aatagcacgt tgcaaagcaa
    10861 tcttagcacg acgttcttgg tcaatcaaat gtttgtcttg tgcttcttca attgcacgtt
    10921 ctgcacgaag tttggcacgt tctgcacgac tgatatcgat atcacgagca cgttctgcag
    10981 agtcagcgac gattgtgatc atatcattgg caatttcaat aacaccaccg tttactgcaa
    11041 tccagttcac ttgatcttta tcatcgatac gttttacctt tacttcatca actgctaaaa
    11101 ccgcaatcat attttcatgt cgtggcaaga tccccatctc accatccaga gttcgaaccg
    11161 atacatagct ggcatggtga tcatagacga gaccatctgg tgtcacgatc tggacagtta
    11221 actgagccat agttcacctc ttaaaatccc attttttcag cttttgcaat cacatcttcg
    11281 atagaaccta caccacggaa ggcatcttct ggcaagtggt cgtatttacc atcaaggatt
    11341 tccttaaagc cacgtacagt ttcagcaact ggaacataag aacctggctg accagtaaat
    11401 tgttccgcaa cattgaagtt ttgtgacaag aagaactgga tacgacgggc gcgagcaacc
    11461 aaggtctttt cttcatcaga aagttcatcc ataccaagga tagcaatgat atcttgcaat
    11521 tcatggtaac gttgaaggac acgttttact tcagcagcaa ctgcatagtg ctcttctcca
    11581 acgatttcag gtgccaaggc acgtgagctt gaagcaagtg ggtcaacggc tgggtagata
    11641 cccaattgta ccaacttacg ttccaagttt gttgttgaat ccaagtgagc gaaggctgtt
    11701 gctggcgctg ggtcagtata gtcatccgct ggcacataga tagcctggat agaggttaca
    11761 gaacccttct tggttgatgt gatacgttct tgcaattgac ccatttccgt agcaagtgtt
    11821 ggttggtaac caacggctga tggcatacga cccaaaaggg cagatacttc tgaaccagcc
    11881 tgagtgaaac ggaagatatt atcgataaag agaagcacgt cttggccttc cacatcacgg
    11941 aagtattcag cgattgtcaa accagtaagg gcaacacgca tacgtgctcc tggtggctca
    12001 ttcatctgac caaagaccat ggctgttttc tcgataacgc ctgattcttt catttcccag
    12061 taaaggtcat tcccctcacg agtacgttcc ccaacaccag caaatactga aataccaccg
    12121 tgctcttggg caatgttgtg aatcaattct tggattaaga cagttttacc aactccggca
    12181 ccaccgaaaa gtccaacttt accaccttta aggtaagggg caagaaggtc aataaccttg
    12241 atccctgttt caaggatttc agaagaggta gacaactcat caaaagttgg agctttttta
    12301 tgaattggct gacgctctgc gtcttctgta aaaggagctt ccaagtcaat ggtatctccc
    12361 aaaacgttga agacacgtcc caaagtttct ttacctactg gtacagagat tggacgacct
    12421 gtgtccaata cttccattcc acgagtcaac ccatctgttg attccatggc gatagtacga
    12481 accataccat ctcctaactc caaggctact tcaaggacga tttttgtttt tctttcgtca
    12541 tttttgtaga cgacaagtgc attgttaatc tcaggaagtt tttcccctgc tgcaaacaaa
    12601 acgtctacaa cgggaccgat aacctgagca attttacctg aactcatctc cttctcctat
    12661 tctatataag atactgtcgg ttcctagctc aactaggtcc taagttcatt ttcatacgag
    12721 ctggactaga gcctattcta aggcactagc acctgctacg atttctgtaa tttcttgtgt
    12781 aatcgccgcc tgtctggcac ggttatactg aattgtcaaa tcattgatga ctttcttagc
    12841 attatctgtc gctgtttgca tggctgtcat acccgcagca ttctcagctg tcttggcatc
    12901 tataatggca ccgtaaatca tactttctgc aaactgaggc aacaactgct ccagaatttc
    12961 ttctcggctg gtttccaatt caaaagtcaa gctgtactct tcatccgctt catttggatc
    13021 caagtcaaca atcggaagca tttgttccac acgcatttga ctggttagcg tattgacatg
    13081 gtggttgtag caaacataaa gctcatcaaa gagttcattt tggtacattt caacagtttt
    13141 tgaaataatc ttacgaactt gatcaaagct aggttggtct gccaagccac gtaattcata
    13201 aagtggttga ataccgcgag ccttaaagaa atcagctccc atcccaccga tacagatcat
    13261 ttcaaaacct ttaccgtctg ggtggtattc ttctttcaac tccataacag ctttcaaaat
    13321 agaggaatta taacctccaa ccaaaccgcg gtctgaagtg ataacgatat agcctgtctt
    13381 cttcacagaa cggctaatca acatcggatt agttgaagca ccagctccat taccatgaag
    13441 gatatctgtc aaaagtttac gcactttctg agcgtaaact tggaagttgc gagcagcttc
    13501 ttcagagcga cctagcttag cagccgatac catttgcatg gcattagtga tttgactcgt
    13561 attttttgtt gaggcgattt ttgttttaat atcatttaga gatactgcca tctgacacct
    13621 ctattcttat tggaagctag attgattgag aaactctgta atcgcagcat ccaagactgc
    13681 ttcttctggc aagtcttttg tatcacgaat ggtttccaaa atctctggat gttgagcatc
    13741 aaagaaggca tggaactctt cctcgaaacg aacaatatca tctactggaa cagtatccaa
    13801 gaaaccatgt atcaaagcat aaagaatggt tacttgtttc tcaacaggta atggtttgtg
    13861 aacaggttgt ttcaagacct caacggtacg acgtccacgg ttcaacttag cctgtgttgc
    13921 tgcgtccaag tcagaaccaa acttagtaaa ggcttccaac tcacggtatg aagcaaggtc
    13981 gatacgaagt gtaccagcaa ccttcttcat ggctttgatt tgtgcagaac cacctacacg
    14041 agatacagat gaacccgcat cgatggctgg acgaatacct gcattgaaga ggccatcgcc
    14101 aaggaagatt tgtccatcag tgatagaaat cacgttggtt gcgatatagg ctgagatatc
    14161 tcctgcttgt gtctcgataa atggtagggc tgtaattgat ccaccaccaa gttcatcaga
    14221 aactttagct gagcgctcaa gcaaacggct gtggagatag aaaacatccc ctgggaaggc
    14281 ttcacgacct ggaggacgac gaagcaagag cgacagttca cgataagcta ccgcttgttt
    14341 tgatagatca tcgtatacaa tcaaaacatg cttaccttga tacataaatt cttccgccat
    14401 agcgacccca gtataaggag ctaggaagag caatggagat ggttgtgaag cagaggctgt
    14461 cacaacgatt gtgtagtcca aggcaccgta ctgacgaagt gtttctactt gcgtacgaac
    14521 tgttgattct ttttgtccaa tcgcgacgta gatacagatc atatcttgat ctttttggtt
    14581 caagattgta tcaatcgcaa tggttgtttt ccctgtctga cggtcaccga taatcaactc
    14641 acgttgacca cgaccaatcg gtacaagggc gtcaatagct ttcaaaccag tttgcaatgg
    14701 ttctgaaaca gacttacgtt gcataacacc aggagctggt gcttctactg gacgagtttt
    14761 atcagtgtgg atttctccaa gaccgtcaac tggacgacca agcggatcca caacacgacc
    14821 aatcagactt tcacctacag ggacttccat gattttccct gtacggcgga ttgtatcgcc
    14881 ttcacggata tctgtaaagt cacctaggat gataatacca acgtctgttg actccaagtt
    14941 ttgagccata ccataagagc cgttttcaaa attcaacaac tctccactca tgacattttc
    15001 aaggccgtga gcacgcgcga taccgtcccc gatataggtt acaacacctg tttcagtcac
    15061 atcaaaattg ggtttgaaat tttcaatttg ttgcttaatt aaagcgctga tttcttgtgc
    15121 gttaattgcc aaaagaacac cactttctat ttcaaatttt ctttaacaac tttaagttgt
    15181 tgtttaatac tcacatcaat tgtcttgtga ttggcaaaaa tgacaaaacc accaatgaga
    15241 ctttcatcga tttgttcttt tacactcctt actttcagag acattttttt ctcaatcaaa
    15301 gggagcaaac gagtcttctg ttcatcagtt agaggatgag cagacgtaat cgtcacttca
    15361 aatcgatttg tttctttttc aagtcggttc aagcaatcta caagcacatc ataaaaaaga
    15421 tttgctctgt gattgtaggc cagaacctgg ataaagtttt gtaataaagg cgacacagaa
    15481 tcttggaaaa aagcaattgt tttttcctta tccgactcgt ctactgccac ttgttttaaa
    15541 aaagaaggca gacctgtttt ttcaacaact tgcttgattt gagtcaagtc tgaaaagata
    15601 cggtcttctt ctcctttttc aagtaccaat tggacaaaag gcatgctgta tttttcaatt
    15661 acctttactg ttttcttgtc cattaagctt ctcctagctg atcgatatac tgatcaatga
    15721 gtgctttatg ggcatgactg tcaaggtttt gtgagatgat tttaccagct aagctgatgg
    15781 tcagatctgc gacctcaccc ttaacactct gtaaagcttc tactttattt tgagcaattt
    15841 cttgattggc tttttctttt aagtgtcctg cttctagttt agcatctgct aagatattag
    15901 ccttactttg ctcagctgtt tcctttgcat tttcaatgat tgtcttagct tctttacggc
    15961 taccagccaa ttcatcttcg cgtttttgag ccaatacttc tgctttttga cgggcttctt
    16021 cagctctgtc aatatctgaa gcaatttttt cagctctttc ttcgaaaatg cctgtaatat
    16081 tagaccatgc aaatttttta atcaagacta gcaaaagaat aaaagagcca gtgattaaaa
    16141 taaaattacc aattaattca cctactgtta cgtgcatcag ttactccttt ctattcctct
    16201 tcaatattaa tcttattccc taaatacaca gatgtcaaaa gagtaaaaac ataagcttgg
    16261 atgcaggaaa taaagacaga aaatgcagtc caagccaaat tagctccaaa ggctactgga
    16321 taccaataaa tagcttggtg ggaaagaaga agtaacaaac tcgtcatgac ctctcctgca
    16381 aagatattcc caaaaatccg caaagccaaa gataagaagt ttgtaaattc ttccaagata
    16441 ttcatcggta tgacaaaaac aggactcata aaagatttta tacttttttt aaatccacga
    16501 cgacgaacgc tttctatatg tgtcaacaaa atgacaagaa aagataaggt taagtcatac
    16561 tgtaaattag cggttggcga actccaccag ttagtcccat cgatcgtttg aagctttgtc
    16621 attaagccaa ggttattggc aatcaccatg aaaaggaata aacaaaggaa aaagagtgaa
    16681 taatctttca tgtagcgcga accaatgtta ggttctgtaa atccaataac aaagtcatag
    16741 acatactcaa gtacattttg ctttcctttg ggtttcaagg tcatattgcg gcttgcccaa
    16801 taaataaaga caaaaataac tcccacaatc aacaaagtca tggctaacat agtcagattg
    16861 aagataacag gaccaataga gatgattgga ttaatacttt cttccatcta aaatcctccc
    16921 ttttcttctc gttccatgta tttatttgat aataaatgag aagacaagag ttacaaagaa
    16981 agttccttca ataaaggcaa cacctaaaaa catcaaacta cgaaactcag aaagcatatc
    17041 tggttggcgt gctactgatt taaacagtcc attcatcaat aaaccttcac cgacagatac
    17101 gcccatacag gcaatacata agcctaaaaa tgttaaattc ataacaaatt ctcctttaaa
    17161 ataaaattta cttacttagt gtagtcctaa aatttaaatt tgtcaacttt taaccgctat
    17221 tggaagcgtt taataaaaat tatttccatt ttgctaaatt tcaagttcaa gttagccatc
    17281 ca
//
LOCUS       DAVBHS010000030        17268 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVBHS010000030 DAVBHS010000000
VERSION     DAVBHS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17268
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.030"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..965)
                     /locus_tag="VQ651_000722"
     CDS             complement(<1..965)
                     /locus_tag="VQ651_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET2753745.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTVTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPVENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKNFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQE"
     gene            1037..1414
                     /locus_tag="VQ651_000723"
     CDS             1037..1414
                     /locus_tag="VQ651_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HET2753746.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELMMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            1505..1837
                     /locus_tag="VQ651_000724"
     CDS             1505..1837
                     /locus_tag="VQ651_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HET2753747.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            1849..3420
                     /gene="ffh"
                     /locus_tag="VQ651_000725"
     CDS             1849..3420
                     /gene="ffh"
                     /locus_tag="VQ651_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET2753748.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(3615..4898)
                     /locus_tag="VQ651_000726"
     CDS             complement(3615..4898)
                     /locus_tag="VQ651_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HET2753749.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(5152..5865)
                     /gene="rsmG"
                     /locus_tag="VQ651_000727"
     CDS             complement(5152..5865)
                     /gene="rsmG"
                     /locus_tag="VQ651_000727"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET2753750.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            5959..6519
                     /locus_tag="VQ651_000728"
     CDS             5959..6519
                     /locus_tag="VQ651_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET2753751.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTSEEEKSVPKVDFSGLGD"
     gene            6521..7420
                     /gene="htpX"
                     /locus_tag="VQ651_000729"
     CDS             6521..7420
                     /gene="htpX"
                     /locus_tag="VQ651_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET2753752.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(7464..9023)
                     /locus_tag="VQ651_000730"
     CDS             complement(7464..9023)
                     /locus_tag="VQ651_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2753753.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKTAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLAQELQLTVRGQEKIGIIGPNGVGKSTLLAKLQQLLNDKREISLGFMPQDYHK
                     KLQLDLSSIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(9262..9579)
                     /locus_tag="VQ651_000731"
     CDS             complement(9262..9579)
                     /locus_tag="VQ651_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET2753754.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSPLIGLGSLKLNDQARTAWRNYDKIHYEHVKHVLSLYGPV
                     FGF"
     gene            9758..10300
                     /locus_tag="VQ651_000732"
     CDS             9758..10300
                     /locus_tag="VQ651_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET2753755.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            10300..10929
                     /gene="nth"
                     /locus_tag="VQ651_000733"
     CDS             10300..10929
                     /gene="nth"
                     /locus_tag="VQ651_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET2753756.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            11140..11661
                     /gene="pyrR"
                     /locus_tag="VQ651_000734"
     CDS             11140..11661
                     /gene="pyrR"
                     /locus_tag="VQ651_000734"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET2753757.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            11680..12603
                     /locus_tag="VQ651_000735"
     CDS             11680..12603
                     /locus_tag="VQ651_000735"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET2753758.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            12653..13732
                     /gene="carA"
                     /locus_tag="VQ651_000736"
     CDS             12653..13732
                     /gene="carA"
                     /locus_tag="VQ651_000736"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET2753759.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            14044..17220
                     /gene="carB"
                     /locus_tag="VQ651_000737"
     CDS             14044..17220
                     /gene="carB"
                     /locus_tag="VQ651_000737"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET2753760.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         5064 a         3349 c         3886 g         4969 t
ORIGIN      
        1 tcctcttgtg aaaaggtaat ggatttaaag accttgcggt tgacaaagcg ttgagataaa
       61 tctgcaagaa ttttgtcagg actagtcatc caaagctgga agtaggtatt catcacgcca
      121 tcatccagag ccagatagtc agtcaaggtc acattttttt cgaagaaagg caggagatgt
      181 ggggaagttc gtgcaaagaa attcttgtcc tcagggtaga gttccttggc gcgcttgaga
      241 agattctgta ggagaacttc catggcgcgt gttgctgggt ggaaataaac ctgcatgtac
      301 atctggtagc gactgaggac gtagtcttcg atggcgtgca tgccattgcg ctgaaaggcg
      361 ataccatttt cgacaggacg aatgactcgg aggatgcgag tcaggtcaaa ttccccatag
      421 gatgctcctg taaaatagga gtcgcgcaag agatagtcca tgcgatctgc gtcaatctga
      481 ctagaaatga gctgcacgac ctgcttatta ggataggtat ggtcaatgac actggccacc
      541 ttttctggga aatcaggtgc cacttgtagc aggacttggt gaatctctgt ctcaggattt
      601 tgaataatct cctgagtaat ggcttcatgg tctgtatcaa agagattttc aaaagtatgg
      661 gagtaggcac catgcccaag gtcgtgtagg agagcagcgg tcacggtcaa gagagactcg
      721 gcaggattcc attcctcagg atatttttct tcgaaaatct ctgtgatgcg tcgtgcaatt
      781 tcatagactc ctagacagtg agagaagcga ctgtgttctc caccgtggaa ggtataactg
      841 gaagttccca gttgtttgat ccggcgcaaa cgctgaaatt cttttgtatt aatcaagtca
      901 tagatgattt gattattgac atggatgtag ttgtgaactg ggtcacggaa tactttttcg
      961 ttcatatgac cattatagca aaatcctgct ctttttggta aaatagtagg acaagagaca
     1021 agaaagaggg cctgatgtga agattcggat gcgaaataca attcagtttg atgagcagtt
     1081 ggaagtgatt gaccagcttt atgatgtgga agtgcatgaa aaaggagatt atagctacct
     1141 gcttttctat aatgaggaaa aggaaaaagt agtgattaaa tttcatggtc aagaacttat
     1201 gatgagccga ttttctaatc ccaagaccat tatgcgcttt ctaaaggata gcgatagttt
     1261 agcctatatt cctacaccta tgggcatgca ggagtttatc atccaaacga gccattatca
     1321 agttgatagg caaaagattg aactagatta tcaactacaa aatcaagagg gacatccttt
     1381 tgccagctat caattggaaa ttacttgggg ctaggctcat gtctttttca aatttagcta
     1441 tctatcttct tggatcggct tcctagtgtg gtagattagg ctaatttttg gtataataaa
     1501 agttatggaa atcgaaaaaa ccaatcgtat gaatgcgctc tttgaatttt atgcggcgct
     1561 tttgacagat aagcaaatga attatatcga gctctactac gctgatgatt acagccttgc
     1621 tgaaattgcc gaggagtttg gtgtcagtcg tcaggctgtc tatgacaata tcaagcgaac
     1681 agaaaagatt ctggaagatt atgagatgaa attgcacatg tactcggact atattgtccg
     1741 cagtcagatt tttgatcaga ttttggagcg ctatcccaag gatgactttc tgcaggagca
     1801 gatagaaatt ttaacaagca ttgataatag agaataagag gaagaaaaat ggcatttgaa
     1861 agtttaacag aacgtttgca gaacgtcttt aaaaatctac gtaaaaaagg aaaaatctct
     1921 gaatctgatg tccaagaggc aaccaaagaa attcgcttgg ccttgctcga ggccgacgtt
     1981 gccttgcctg ttgtaaagga ctttatcaag aaagttcgtg agcgtgcagt cgggcatgag
     2041 gtcattgata cacttaatcc tgcgcaacag attattaaaa tcgttgatga ggaactgaca
     2101 gccgttttag gttctgatac ggcagaaatt atcaagtcac ctaagattcc aaccatcatc
     2161 atgatggttg gtttacaagg ggctggtaaa acaacctttg ctggtaaatt ggccaacaaa
     2221 ctcaagaaag aagaaaatgc tcgtcctttg atgattgcgg cggatattta tcgtccagct
     2281 gccattgacc agcttaagac cttgggacaa cagattgatg tgcctgtctt tgcacttgga
     2341 acagaagtac cagctgttga gattgtacgt caaggtttgg agcaagccca aactaatcat
     2401 aacgactatg tcttgattga tactgcgggt cgtttgcaga ttgatgagct cctcatgaat
     2461 gagcttcgtg atgtgaaagc attggctcaa ccaaatgaaa tcttgcttgt cgttgatgct
     2521 atgattggtc aggaagcagc caatgttgcg cgtgagttta atgctcagtt ggaagtgact
     2581 ggggtcatcc ttaccaagat tgatggcgat actcgtggtg gtgctgctct gtctgttcgt
     2641 cacattactg gaaaaccaat caagttcact ggtacaggtg aaaagattac ggacattgaa
     2701 accttccacc cagaccgcat gtctagccgt atccttggta tgggggatat gctcactttg
     2761 attgagaaag cttctcagga atacgatgaa caaaaagccc ttgaaatggc tgagaagatg
     2821 cgcgaaaaca cctttgattt taatgatttc atcgatcaat tagatcaggt gcaaaatatg
     2881 gggccgatgg aagacttgct caagatgatt ccaggtatgg ccaacaatcc agcccttcaa
     2941 aacatgaagg tggatgaacg ccagattgct cgtaaacgtg ccattgtgtc ttcgatgaca
     3001 cctgaagagc gtgaaaaccc agatttgtta aatccaagcc gtcgccgtcg tattgctgct
     3061 ggttctggaa atacattcgt cgaagtcaat aaattcatca aggactttaa ccaggctaaa
     3121 cagctcatgc agggtgttat gtctggggat atgaataaaa tgatgaagca aatggggatt
     3181 aatccaaata accttcctaa aaatatgcca aatatgggag gaatggatat gtctgccctt
     3241 gaaggaatga tgggacaagg cggtatgcct gacttatcag ctctcggagg agcaggaatg
     3301 ccagatatga gccagatgtt tggtggcggt ttgaaaggta aaattggtga atttgccatg
     3361 aaacagtcca tgaaacgtat ggctaacaaa atgaagaaag cgaagaagaa acgcaagtaa
     3421 gacaagagat tgatgcaaat ctcatttgat gagaaaataa agtaaaaatt tctatgataa
     3481 aacgtacagt atcaaggttt tagaatatct gatattatac gttttttgat tttaataatt
     3541 ttgttaagac acttttatta tggtatacga aaaatccact cagctgtctg agtggattag
     3601 gtgtatttag actcttagtc tttattttcg tatggcaaga tcaagttcaa gatgattcct
     3661 gtcatggctg aaagggcagt acctgaaagt gtaactggac caagtttaag gatagctcct
     3721 ccaagtccaa gaaccaacat agcacttgcg atgatgaggt ttcgcatttg agcgaaatca
     3781 acacgttctt taatcaagac tttcaaacca ttgctggcga taaccccata gagaaggatt
     3841 gacataccac caagtacagc gtttggaata gttgaaatca aggcagtgaa tttaccaagg
     3901 aagctgaggg caatcgcgat gaaggcagcg ttacggataa ctgagacaga agcgatacga
     3961 gtcataccga taacccctgt attttctccg taagttgtat tggctggtcc accaaggaag
     4021 gcagaaacag aagttgcgat accgtcacca agaagagtac ggtgaagacc tggttctttt
     4081 aagaattgac gaccacagat ttgacccaaa acagtatggt ctccgatatg ttcagaaatt
     4141 gttacgatag cgattggcaa gatagcgatg gcttctggac caaagtaaag attgtactct
     4201 ttaaaggcac cacctgtgct aaatggcaag tagaaaccag gaatttcgaa ccagttggct
     4261 ttaagaactg gtgtaaagtc aaccaagcca agagttagtg cgaaaaggta accaccgata
     4321 atggcaaaga ggaatggaat gattcgtagg aagccttttc cttttgtatt gataaaggca
     4381 gcaattagga aagtaacaac ggctaccaga gcatttttcc aatttccgtc tgctacaaga
     4441 cctgcattgg taacagctga acctgcaagt ccaagaccga taacgatgat cataggaccg
     4501 ataatgattg gtggcaagag tttatcaatc cattttgttc ctacaaatcg gatgctggta
     4561 gcaacaagga catagaccaa accagtcaag ataacccctg tttgggcagc agatacatcc
     4621 ccccccattt ctttcatagc cagtgacata gctgtgataa aggcaaatga agaacctaga
     4681 taaactggaa ctttaaaacc agtagcaatc atgtagatga gtgttccaac acctgaagca
     4741 aaaagggcaa cagatacagg cattcccaaa atcaatggta ccaagatggt cgcaccaaac
     4801 atggcgaaaa cgtgttggaa actaaggaga attccttttc cagccgaagg acgttggtca
     4861 acgtctagta acaaatcaac agttgattct tgtttcatat aaacctcact tttgggcatc
     4921 aaaaaagagc ccattatttt acagcaatag gccctcagag taagacttct ttaaaattta
     4981 gattttaaag agttttaaat atttctgcgt cttcgtcacc tcacgggatg acattaaaaa
     5041 cctttgctta ggatagtata gcagaaaaaa atgattttgt aaatcatttt ttcccgagcc
     5101 tagaaataaa agagcgaggc tgattttgta aacatttgtt tactaaaaaa tttaaagtgg
     5161 gcgtttattt ggcataccag ccttacgcgg atatttattt ggtgtttctt ttttcttttc
     5221 taccactgtg atatagcgcg gatctctatt cggtagggcg tagctgagat tgtcttcgac
     5281 cttactaaaa aggagattga gggcattctt agcttctaat aattcctcag gcgcattgct
     5341 ggccttgagt gccaatagtt ttccaccaac cttaaggtag ggaatggtca attcagatag
     5401 gacctgcata cgggcaaccg cacgagctgt tacaaaatca tattgagcac ggaagttctt
     5461 gtcttgggca aaatcttcgg cacgtccgtg gtagaaatga actccgttca aatccagttc
     5521 ttgagccaag agttgtagga agttgatgcg cttattgagt gaatcaataa tggtcacatc
     5581 taactccgga tagagaattt tcattggtag actaggaaat cctgccccag ccccgatatc
     5641 aagaagtttg atagtttcat tgggaatcaa accttgaaga atgggtgcaa tcgaatcgta
     5701 aaaatgtttg agataaactt cttccttgtc cgtaatcgcc gtcaaattaa tcttctcatt
     5761 ccactcgacc aagagctcaa aataacgttc aaattgttct ttttgctggt ccgaaagtgg
     5821 aagattctgc tcggcaagca agttgtaaaa tgtttttggt ttcatagtta tttccttctt
     5881 tagtatcatc ttattttacc acaatcatag aaaatttgat atactggtac taatctaaaa
     5941 gcagaaagga cttatattat gacttggatt attcttggag ttatcgctct tattgttatt
     6001 tttgtgattg ttagctataa cggtttggtt aaaaatcgta tgcaaaccaa ggaggcttgg
     6061 agtcagattg atgttcagtt gaaacgtcga aatgacctct tgccaaactt gattgagact
     6121 gtaaaaggtt atgccaaata tgaaggttct acccttgaaa aggtggcaga actacgtaac
     6181 caagtggcgg cagcgacttc accagcagaa gctatgaaag ccagtgatgc cctcactcgt
     6241 caggtttcag gtatttttgc agttgcagaa agctatccag atttgaaagc tagtgctaac
     6301 tttgttaaat tgcaagagga gttgacaaac acagaaaata aaatttctta ctctcgtcaa
     6361 ctctataaca gtgttgtcag caactacaat gtaaaattag aaactttccc gagcaatatt
     6421 atcgctggaa tgtttggatt taaagcggca gatttccttc aaacatctga agaggaaaag
     6481 tcggttccta aagttgattt tagcggttta ggtgactaag atgttgtttg atcaaattgc
     6541 aagcaataaa cgaaaaacct ggattttgtt gctggtattt ttcctactct tagctcttgt
     6601 tggttatgcg gttggttatc tctttataag atctggactt ggtggtttgg ttatcgcact
     6661 gattatcggc tttatctacg ctttgtctat gatttttcaa tcgacagaga ttgtcatgtc
     6721 catgaatgga gcgcgtgagg tggatgagca aacggcacca gacctctacc atgtagtgga
     6781 agatatggct ctggtcgctc agattcctat gccccgtgtt ttcatcattg atgatccagc
     6841 cttaaatgcc tttgcgacag gttctaatcc tcaaaatgcg gctgttgctg cgacttcagg
     6901 tctactagct atcatgaatc gtgaagaact agaagctgtt atgggacatg aagtcagtca
     6961 tattcgtaat tatgatatcc gtatttcgac tattgcagtt gcccttgcta gtgctatcac
     7021 catgctttct agtatggcag gtcgtatgat gtggtggggt ggagcaggtc gcagacgaag
     7081 tgatgatgac cgagatggaa atggtcttga aatcattatg ctagtggttt ccctactagc
     7141 tattgtactg gcacctctcg ctgcaacctt ggttcagctc gctatttctc gtcagaggga
     7201 atttctggca gatgcatcta gtgtcgagct gactcgcaat ccccagggaa tgattaatgc
     7261 cctagataag ttggacaata gcaaacctat gagtcgccac gtcgatgatg ctagcagtgc
     7321 cctttatatc aatgatccta agaaaggtgg ggggttccaa aaactctttt atacccaccc
     7381 acctatctca gaacggattg aacgtttaaa acagatgtaa aagaatccag aggtcgtctc
     7441 tggatttttg ctatgttgca aatttataaa tcttctaaat taactagctt caaaccgtgt
     7501 tctgtcatgc gatagatgat cgagcagact tcttttaaga aacgacggtc atgcgaaaca
     7561 gtgatgagac cgcctggata ggttgcaaag agttttctga tttggggttg agaagtggga
     7621 gaaaagtttc gtgtgggttc atccagcagg agaaagtttg gtttgcgcag aactagatcc
     7681 aaaagcagga gttttccctg ttgtccgcca gataaggagc gaatttgatg ctgcatttct
     7741 ggataactga aattgagact ggctaggtga gattggattt tctgaagttc ctctttttcc
     7801 ccagttttgc tgatataagc tattgaggat aaatccaatt gcagtttttt gtggtaatct
     7861 tgtggcataa aaccaagtga aatctctctt ttatcattca gaagttgctg taatttggct
     7921 aacagagttg atttcccaac accatttggg ccgatgatac cgattttttc ttggccacgg
     7981 acagttagtt gcagttcctg agccaaaaca cggtcgtcaa tggacaaatt ttctttttcc
     8041 agttggacta agactttaga agctggtaat ggttggatgt ctgaaaagaa aagttggatt
     8101 tgttcctctt caagtggctt ttgagtcatg gactgagctg tcttttcgta gcgtttttct
     8161 tgtgagagga cagttttcat ctttttagcc aataggcgac cggcagtact atctttggta
     8221 gctcgaagcg cagtttctac attttgctta actctccgat gtttttccat ggttttatcg
     8281 taggctcttt ggttgttagc agcttgctga ctttgtttgg caaaattagc ctttctctgc
     8341 tcactatagc tatcatagtc taaatgctct actagcgttt ccgcttcttt acggtgtttg
     8401 accagtcgca agtgaacaat agtgtctgcc gtttcagaaa gaaagtcttc atcatgggaa
     8461 atgaaaataa cggtttgcct ggtcttttga atctggcctt ttagccaatc aactgtctca
     8521 aggtctaggt catttgaagg ttcatctaaa aatagaatct caaagggttt ggctaactca
     8581 tggataagct gaattttcaa agcttcgccc cctgatagat tgccaatctc ttggtcactt
     8641 gcgaaacgat tgctatcaaa atgcaattcc tccgccaaac gatagaggat actgtagtct
     8701 aaatcaatag aatctaaaaa gaagtagtcg tgtaaagttt tcttttttag gtcctcaggg
     8761 actttttgag gaatgtaggc cagtgactga taatcagact ggatgtctcc cttgatagtg
     8821 aaatcagaca aatcttcccc cattaaagtt ttaagtaagg ttgatttacc atttccttct
     8881 tcgccaataa tagctacctt ttccccgtct tgtatggtca tgcttaggtc agagacaagg
     8941 tctcgtaaat ctttgttttg tgtgatggtc aggtgattga tttttatcat actattgccc
     9001 tttctagaat gtcgatagtg cataacaaaa agctctactt tacctagtag agcccaaagt
     9061 ctatgtcaaa aaaactctat taacatgcca gaaaaagagc ataaaagagc acacaaaaag
     9121 agacaaaaac aagatctact aaataaaggt tctttatttg atagacttag ttgttcatgt
     9181 ctcccttacc tccgagtatt agtatcttta tactatactt ttaaggaaat tttgtcaaca
     9241 gaaaactgga aattctatgc tctaaaatcc aaaaacaggt ccatagagac ttagtacgtg
     9301 tttgacatgc tcgtaatgga ttttatcata gtttcgccaa gcagtgcgtg cttgatcgtt
     9361 tagttttagg ctacccagtc caatcagagg actggtgggt tggtaaaatt tctcaagatc
     9421 aattaagata ctgttgttca acttttccgt ttttttgagc tcttgcatag tagtgtttag
     9481 cagctcttgt agtcgaatat catctgccgt accctgttta cagttttggt agcttttatt
     9541 taacttggat aggagttgaa tgagtttctt gtcttccatg tcttccatat gagcatcctc
     9601 cttgatctga tgtactcttt ccctatagta tagcactaaa aaataaaact gtcatagtaa
     9661 atatgttaaa attttgaatt ttaattacaa agtttggtgt gctgatttgt agccttttca
     9721 tggtataatc aagagagtaa atctttatgg aggaagtatg aaattaaata ttcaagaaat
     9781 tcgtaagcag tctgaaggtt tgaactttga acaaacgtta gatttagttg atgacctgcg
     9841 tgcacgtaat caagaaattt tagatgtaaa agatatcctt gcagttggga aagtacaata
     9901 tgaagaccgt atgtatttct tagattatca actatcttat accattgttc ttgcttcgag
     9961 tcgcagtatg gagccagttg agttagttga atcttatcca gtcacggaag ttttcatgga
    10021 aggcgcaact aaccagctag atcaagaagt tttagatgat gacttggtct tgcccattga
    10081 aaatggggag cttgaccttg ctgagagtgt atcagacaat atcctgctaa acattcctat
    10141 caaggtcttg acggctgaag aagaagctgg tcaaggattt atctcaggaa atgactggca
    10201 aatcatgaca gaggaagaat accaagctca acaagcagta aagaaagaag aaaacagtcc
    10261 ttttgctggc ttacaaggac tatttgacgg agatgaataa tggtcttgtc aaaaaaacga
    10321 gcacgaaagg tgctagaaga aatcattgcc ctcttcccag atgccaagcc tagtcttgat
    10381 tttaccaatc attttgaact cctggttgcg gtcatgttat cagcccagac gacagatgca
    10441 gcggtaaata aggccacacc aggtctcttt gttgcctttc caacacccca agctatgtct
    10501 gtagcgacag agagtgagat tgcttcacat atttctcgct tgggattgta tcggaataaa
    10561 gctaaattcc ttaaaaaatg tgcccaacag ttactagacg attttgatgg tcaagtccct
    10621 cagacacgtg aagaattgga gagtttggca ggtgttggtc gcaagacagc caatgttgtc
    10681 atgagtgtag gatttgggat tccagccttt gcagtggata ctcatgtgga gcgtatttgc
    10741 aaacaccacg atattgtcaa aaaatcagcg acgccacttg aggtggaaaa gcgggtcatg
    10801 gatatcttgc cgcctgagca gtggttagct gcccatcagg ccatgattta ttttggaaga
    10861 gccatttgcc atccaaaaaa tccagagtgt gaccagtacc cacaattata tgattttagc
    10921 aatttgtaag atgctacatt gaaacttttt gcttgatttt aagcatgctt tgtgctatag
    10981 taaatgataa ctaaatattc ctttaaacct gtcccgtgag gcaggcaagg agcgcgataa
    11041 agtagaaggg tgaagtctat actgtttgca gtagggctcg cttggctata aatttcaagt
    11101 ctccttgttt aaggagactt ttctttttgg aggtttgtca tgaaaacaaa agaagttgta
    11161 gacgaattga ccgtcaaacg agcgattacg cgtattactt atgagattat cgaacgcaac
    11221 aaagatttga ataagatcgt cttggctggt attaaaactc gtggtgtctt tattgcccac
    11281 cgaatccaag aacgtttgaa gcagttagaa aatctttcag ttcctgttgt ggaattggat
    11341 actaaacctt tccgtgatga tgttaaaagt ggagaagata cttctttggt ttctgtcgat
    11401 gtgacagacc gcgaagttat cttggtggat gatgtgctct atacaggtcg taccatccgt
    11461 gctgctattg ataatattgt cggtcatggt cgtcctgcgc gtgtgagttt agcagttcta
    11521 gtcgatcgtg gacatagaga attgccaatc cgtccagatt acgttggaaa aaatatccca
    11581 accagtcgtt ctgaagaaat catcgtagag atgacagaac ttgatgacca agacagagtt
    11641 ctgattactg aagaagctta gaaagttaaa ggagtagcca tgtcagaaaa tcaacaagca
    11701 ttgaaccatg tggtgtccat ggaagacctc actgtcgatc aagtaatgaa attgatcaag
    11761 cgaggaattg agtttaaaaa tggagcccag cttccctatg aagaccatcc gattgtttcc
    11821 aatctcttct ttgaggattc tacacggaca cataagtcct ttgaagtcgc agagattaaa
    11881 cttggattgg aacgacttga ctttgatgtg aagactagtt cggttaataa gggtgagaca
    11941 ctttatgaca ccattttgac tctgtctgct ttaggagtgg atgtctgtgt gattcgccat
    12001 ccagaggtcg actactatag agaattgatt gctagtccaa cgattacgac ttccatcatc
    12061 aatggtggag atggttcggg ccaacaccct agccagagct tgcttgattt gatgaccatt
    12121 tatgaggaat ttggccactt tgagggtctt aaagttgcta ttgcaggtga cttggaccac
    12181 tcacgcgttg ccaaatccaa tatgcagatt ttgaaacgct tgggagctga actctttttc
    12241 gctggacctg aggaatggag aagtcaagag tttgcagact atggaaagtt tgtaaccatt
    12301 gatgaaatca ttgatcaggt tgatgtcatg atgtttctcc gtgtgcaaca cgaacgccat
    12361 gatagtggag ctgtattttc aaaagaagac tatcatgccc aacatggctt gactcaagaa
    12421 cgttacgatc gtttgaaaga aacagcaatc ctcatgcacc cagctccaat caatcgtgat
    12481 gtagaaatag cagaccactt ggttgaagca ccaaaatcac ggattgtcca acaaatgacc
    12541 aatggtgtct ttgttcgaat ggcaatctta gaatccgtac tagcgagtag aaacgccaac
    12601 taaaacacaa gacgttaaaa gacgccagtg agcgtccacg agaggtgaaa atatgacaaa
    12661 aagaattcta gtattagaag atggcacagt ttttgaaggc aaggccttcg gagcagatat
    12721 tgatgtaaca ggcgaaatcg tctttaatac agggatgacc ggctaccaag aatccattac
    12781 agaccagtct tataatggac aaatcttgac ctttacttat cctttggtag gaaattatgg
    12841 tattaatcgt gatgattatg aatccattat tccaacttgt aagggagtcg ttgtttttga
    12901 agaagcgcgt agagctagca actggcgcaa ccaaatgacc ttggatgaat ttttgaaagc
    12961 caagaaaatt ccagggattt caggaattga tacgcgtgct cttaccaaga ttatccgtaa
    13021 gcatggtact atgcgtgcaa ccttgaccca tgttggggac agtatggacc atgtgacgga
    13081 ccagctccaa gcaacagttt taccgacaga caatatcaaa caggtttcta ctaaaacgtc
    13141 ttatccagct ccaggagttg gtttgagcgt ggtgctagtg gactttggtc tcaagcactc
    13201 aatcctacgt gaactttcta aacgcaactg taacgtgaca gttgttcctt attcgacaac
    13261 ggctgaagaa attctccatc tcaatcctga tggagttatg ttgtcaaatg gtccaggtaa
    13321 cccagaagac gttccacaag cactcgacat gattcgcggt gtacaaggaa aaattccaat
    13381 ctttggtatt tgtatgggac accaactctt tgcaatggca aacggggcta agacctataa
    13441 gatgaagttt ggccaccgtg gattcaacca tgcggtacgt gaaatcgcaa caggacgagt
    13501 agacttcacc agccagaacc atggttatgc agtcagccgc gaggatttgc cagagcactt
    13561 gattattacc cacgaagaaa tcaatgacaa gtcagtggaa ggtgtgcgcc acagatacca
    13621 acctgctttc tctgttcaat accacccaga tgcagctcct gggccacacg acgctagcta
    13681 cctatttgac gagtttatcg agatgatgga agtttttaaa caatcaaact aagaatgagt
    13741 aaaagctcgt cggagaattt atgtaactga acatggacgg aaagctcaga atttagagaa
    13801 accaacttgg attgtcaatc cttccttgat ttcctaaaat tcaatcgctt tctagtcgtc
    13861 ctttgtatct tatttaatgt cgccctgaga ggcgacgaat agaaaggaga agatacattg
    13921 ttcgcggaag tgaacacgcc ctaagaactg tgtgaaaaag ataaacatcg tcttgacgct
    13981 gaaggcgtct gcgccgagtt tcctattttc actgtgttct ttacgggctt tgtatcataa
    14041 actatgccta aacgtactga tattcaaaaa attatggtga ttggttctgg tccgattatt
    14101 attggtcagg ctgctgagtt tgactacgct gggactcagg cttgcttgtc gttgaaagag
    14161 gaaggttatg aggttgtttt ggttaactca aatcctgcaa ccatcatgac ggacaaggaa
    14221 attgctgaca aggtttacat tgaaccgatt acacttgagt ttgtgacacg tattcttcgt
    14281 aaggaacgtc cagatgcctt gctaccaaca ctcggtggtc agacaggtct caacatggcc
    14341 atggaattgt ctaaaaatgg tatcttagat gagcttggcg ttgaacttct gggtaccaaa
    14401 ttatctgcca ttgaccaagc ggaggaccgt gacctcttta aacaattgat ggaagagctt
    14461 gaacaaccca ttccagaatc tgaaattgtt aacacagtag aagaagcagt tgcctttgca
    14521 gcgacaattg gctacccagt catcgttcgt ccagccttta ccctcggtgg tactggtggt
    14581 ggtatgtgtg ccaacgagaa agaattgcgt gaaatcactg aaaatggttt gaaattgtca
    14641 cctgttaccc aatgtttgat tgagcgttcg attgccggtt tcaaggaaat tgaatacgaa
    14701 gtgatgcgtg actcagctga caatgccttg gttgtttgta acatggaaaa ctttgaccca
    14761 gttgggattc acacggggga ttccattgta tttgcccctg cgcaaaccat gtcagactat
    14821 gaaaaccaaa tgcttcgtga tgcgagcttg agcattattc gtgccctcaa gattgaaggt
    14881 ggatgtaatg ttcagctagc tcttgatcct aacagcttca agtattatgt tatcgaagta
    14941 aaccctcgtg tatcacgttc gtcagccctt gcttctaaag cgacaggata tccgattgct
    15001 aagttggctg ccaagattgc agtaggtttg accttggatg aggttataaa cccagttaca
    15061 ggttcaacct atgccatgtt tgaaccagcc cttgactacg tagttgctaa gattccacgt
    15121 ttcccatttg acaagtttga aaagggtgaa cgccgtctcg gtacacaaat gaaggcgact
    15181 ggagaagtca tggcaatcgg tcgtaacatc gaggaatctc ttctcaaggc ctgccgctcc
    15241 cttgaaattg gggtgcacca caatgaaata cctgaacttg cagcagtttc tgatgatgcc
    15301 ttgattgaaa aggttgtcaa agcccaagat gaccgtctct tctacgtatc agaagcaatt
    15361 cgtcgtggct acacacctga agaaattgca gagcttacaa aaattgatat cttctatctt
    15421 gataaactct tgcatatctt tgaaattgag caagaattgg gtgctcatcc acaagatcta
    15481 gaagttttga aaacagccaa acttaatggt ttttcagacc gtaagattgc tgaactctgg
    15541 ggaacgactg atgatcaagt tcgccaactt cgcttggaaa acaagattgt tccagtttat
    15601 aagatggtag acacttgtgc ggcagagttc gactccgaaa cgccatattt ctattcaacc
    15661 tatggttggg aaaatgaatc tatcagatct gataaagaat ctgtacttgt cctaggttca
    15721 ggtccaatcc gtattggtca aggggttgag tttgactacg caaccgttca ctctgttaag
    15781 gctatccagg cagctggtta tgaagctatc atcatgaact caaacccaga gaccgtttca
    15841 acagacttct ctgtatcaga taagctttac tttgagccat tgacattcga agatgttatg
    15901 aacgtcattg acttggaaca accaaaggga gttatcgttc agttcggtgg tcaaacagcc
    15961 atcaaccttg cggagccatt ggcaaaagca ggtgtgacca tccttggtac acaggttgct
    16021 gacctagatc gtgccgaaga ccgtgacctc ttcgagcaag ctctcaaaga attggatatt
    16081 ccacagccac caggacaaac agctaccaat gaagaagaag cagctcttgc agctcgcaag
    16141 attggcttcc cagttcttgt ccgcccatct tatgtacttg gtggacgtgc catggaaatc
    16201 gttgaaaacg aagaagacct tcgttcttac atgcgaactg ctgttaaggc tagtccagac
    16261 cacccagttc ttgtcgactc ttatatcgtt gggcaagagt gcgaagttga tgccatttca
    16321 gatggtaaaa atgtactcat tcctggtatc atggagcata tcgaacgtgc tggtgtccac
    16381 tcaggtgact caatggctgt ttacccacca caaaccttgt cgcaaaaggt tcaggagacc
    16441 atcgcagact acaccaaacg cttagcaatc ggtcttcact gccttggaat gatgaacatc
    16501 cagtttgtta tcaaggatga aaaagtctac gttattgagg tcaatccacg tgccagccgt
    16561 actgttccat tcctatctaa ggtaaccaat attcctatgg ctcaagtagc gaccaaactc
    16621 attcttggtc aaagtttatc agaacttggc taccaaaatg gactttaccc tgaaagcacc
    16681 cgtgttcata tcaaggcacc tgtcttctcc tttaccaaac tagctaaggt agacagcttg
    16741 ctcggtcctg aaatgaagtc aacaggtgaa gttatgggtt ctgatgctac tttggaaaaa
    16801 gctctctata aagcctttga agcttcttat ctacacttgc caacttttgg taatgtcgtc
    16861 tttaccatcg ctgatgatgc caaagaagaa gccttgaact tggctcgtcg tttccaaaac
    16921 attggttatg gaatccttgc gacagaaggg acagcagcct tctttgctag ccatggcttg
    16981 caagcccaac ctgttggtaa gattggtgat gacgataagg atatcccaag ttttgtacgc
    17041 aaaggaagaa ttcaagctat cattaacaca gtaggaacaa aacgaactgc tgacgaagat
    17101 ggtgagcaaa ttcgtcgttc agccattgaa cacggggtgc ccctcttcac agcgctagat
    17161 acagctaatg ccatgcttaa ggtacttgaa agtcgtagct ttgttacaga agcgatttaa
    17221 atagaaaaat aagatatagc agactgaatt tagaacagta cacaggaa
//
LOCUS       DAVBHS010000031        16890 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVBHS010000031 DAVBHS010000000
VERSION     DAVBHS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16890
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.031"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(92..1072)
                     /locus_tag="VQ651_000738"
     CDS             complement(92..1072)
                     /locus_tag="VQ651_000738"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET2753761.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNMGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLSFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            complement(1074..3326)
                     /gene="pbpX"
                     /locus_tag="VQ651_000739"
     CDS             complement(1074..3326)
                     /gene="pbpX"
                     /locus_tag="VQ651_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HET2753762.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDSFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAVYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKVEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            complement(3330..3647)
                     /gene="ftsL"
                     /locus_tag="VQ651_000740"
     CDS             complement(3330..3647)
                     /gene="ftsL"
                     /locus_tag="VQ651_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET2753763.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(3659..4609)
                     /gene="rsmH"
                     /locus_tag="VQ651_000741"
     CDS             complement(3659..4609)
                     /gene="rsmH"
                     /locus_tag="VQ651_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET2753764.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            4775..4969
                     /locus_tag="VQ651_000742"
     CDS             4775..4969
                     /locus_tag="VQ651_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2753765.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            4985..5527
                     /locus_tag="VQ651_000743"
     CDS             4985..5527
                     /locus_tag="VQ651_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HET2753766.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIVLFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            5538..5780
                     /locus_tag="VQ651_000744"
     CDS             5538..5780
                     /locus_tag="VQ651_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753767.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            5791..6126
                     /locus_tag="VQ651_000745"
     CDS             5791..6126
                     /locus_tag="VQ651_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753768.1"
                     /translation="MKSLARLLNIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(6236..7237)
                     /locus_tag="VQ651_000746"
     CDS             complement(6236..7237)
                     /locus_tag="VQ651_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2753769.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7297..9198)
                     /locus_tag="VQ651_000747"
     CDS             complement(7297..9198)
                     /locus_tag="VQ651_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET2753770.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYVVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDMLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYYFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYRELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSNKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            complement(9220..9513)
                     /locus_tag="VQ651_000748"
     CDS             complement(9220..9513)
                     /locus_tag="VQ651_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET2753771.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQTEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            complement(9513..10331)
                     /locus_tag="VQ651_000749"
     CDS             complement(9513..10331)
                     /locus_tag="VQ651_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2753772.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVVIGIIGSWLKIIA"
     gene            complement(10318..11097)
                     /locus_tag="VQ651_000750"
     CDS             complement(10318..11097)
                     /locus_tag="VQ651_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2753773.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTANHVVEEEAHDDWI"
     gene            complement(11112..11603)
                     /locus_tag="VQ651_000751"
     CDS             complement(11112..11603)
                     /locus_tag="VQ651_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET2753774.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLACNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            complement(11614..12804)
                     /locus_tag="VQ651_000752"
     CDS             complement(11614..12804)
                     /locus_tag="VQ651_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HET2753775.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(12816..13244)
                     /locus_tag="VQ651_000753"
     CDS             complement(12816..13244)
                     /locus_tag="VQ651_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2753776.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPVKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEDFESGI"
     gene            13515..14330
                     /locus_tag="VQ651_000754"
     CDS             13515..14330
                     /locus_tag="VQ651_000754"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HET2753777.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            14349..14990
                     /locus_tag="VQ651_000755"
     CDS             14349..14990
                     /locus_tag="VQ651_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HET2753778.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            15021..16022
                     /locus_tag="VQ651_000756"
     CDS             15021..16022
                     /locus_tag="VQ651_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET2753779.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            16032..16661
                     /locus_tag="VQ651_000757"
     CDS             16032..16661
                     /locus_tag="VQ651_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET2753780.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
BASE COUNT         5016 a         3787 c         3070 g         5017 t
ORIGIN      
        1 tgagcagcct aaagttagct ttctagtttg ttctttgatt ttcattgtgt atttaaacac
       61 aaaaacaccc tgaaacatca gggtgccatt cttacatcaa atacaaaatt gcaagggtca
      121 ggagacttgc tagaagccca actccccaaa agaagaagtc aaccttccac tcgctccaag
      181 gatttccttt accagacaat cccccaagct caaaatggtg atgtacaggc gtcatacgga
      241 aaatacgttt accacctgtc agtttgaaat aactgacttg catcataaca gaagttgttt
      301 caaaaacata tacaattccg ataatcaaga gagtccattc ttggtggaga gccatagaga
      361 tagctgccag catcccacct agggccaaac ttcccacatc acccataaag accttagcag
      421 gcttatggtt aaagatgaaa aaactgagca aaccaccaat catggcaaga atcactagaa
      481 gaatatccat ctgaccttgc acataggcaa taactccata ggcagacaaa ctaatcacaa
      541 cggaaatact agctaaaccg tcaacaccgt ctgtcaagtt tactgcgttt gaaaaaccga
      601 ctagccagaa aagagcgaag acaatataga aaatccctag atgcacttga taaccaaaga
      661 cagaaagcat atcgccaccg cgctcataga aaagatagaa gataactcca cctagaagct
      721 gaagagctaa tttttgctta ggattaagcc cctcattgat tttacgaaag accttgagaa
      781 agtcatctaa aaatccgacc aagccataca agaccaagat gaacaaaatc attcccatat
      841 tattgctgaa ttggctacta aatagggcga aaaagaaagc aaccaaaaca gaagtaatca
      901 agaaaaccaa acctcccatt gtaggagtcc cagcttttgc ctgatgctgt ttgacatcct
      961 catgcatctg ctggcctgta atttgcgcct ttctataaaa ttggataaag gccggaattc
     1021 ctactaaagt tagtaaaaat gtcacaattc cagcactgat ggaaataaac atattagtct
     1081 cctaaagtta atgtaatttt tttaatgtcc ttgatagctg tgttagcacg aacatcttgc
     1141 ttctgcacag tagagcccga accttgaaat tcaagttcta tattgagcca cttagcaagg
     1201 gtctcagcag tctcctttgt ccaaccatac atatctggaa cttcctctac tttatcagat
     1261 aagataagga cttgctgatt cggggcaaga ttcttccctt cttcagcaga actgttttta
     1321 atcttcgttc ctgttcccac aacgatgggt tgtacaagat tgcgacgcaa ttcttctgct
     1381 aaatcaccag gtgaaatatc cttgacacta ggcataggat aaggactttg ttgacttact
     1441 tgctccaaag ctttagctgt tgtttgaaga ttgagagagt ctttcatagc tgaagcccgc
     1501 tccaagatag gattggcaaa ttctcccaac tgaatacctg aataatgttc aggttgttgg
     1561 accgtcacat acaagataaa atcaggattt tcagccggac tcatcgatac agccgagaaa
     1621 atatagtcgg ttaacccgac tagataacca ccatttttct cgtcagcaat ctgagccgta
     1681 ccagacttga gggctacatt ttgcccagga acagttacag ttggcttgcc tgtgctgtgg
     1741 ttatacatgg ttccataaac cggatccgtc cctaccaaaa ccatgttagt ccgagttaga
     1801 ctagctgcat ctttagaaac aggatttccc acaatttctt tttgagattt ccgagcagtt
     1861 tgatcatttg gatcataaac ggcactaata aatttaggct ccagcatgac accgtcatta
     1921 gcaatagctg taaaggcacg aatcatttgc gtctgggtca ctgaaatccc ttgtccaaat
     1981 gagctttgcg caatgttgac aatattatcc gcaggaagct gaccagcata ctcatccgtc
     2041 aaaccgaaac gggtcggaac tccaaactta aaacgattaa gataatcaag ccaggtagca
     2101 tctcccatct tttgctcaag gagggtcatc ccaacgttac ttgagtgtgc aaaaccttga
     2161 gaaaaagtca tcattctgcc accagtcaat ccttcattaa cgtcccaatc tcgaatcgtg
     2221 gcatctgcaa tttttaactc actactatta aagacttctc ctcctggaaa ggtattatta
     2281 tcaatagcag cagccaacat catcactttc atagtggaac ctggctcata gttactttgg
     2341 taaaggatat cacgccaaac aaagtcctct gtaatgcctt cttttgtatc tgcatcaaag
     2401 gtcggtcgtt gcgttgttgc cagaatttcc cctgttttag cactgaccaa agtcgctgtc
     2461 atgtactttc cttttacctt ctcttgaaaa gaatccatct gggtttccat aaaggactgg
     2521 agggggctgg aaatggttgt ataaacatcc ttaccgtcca tcgttcgttg ggaaacttgt
     2581 tctgttccgg gtacaatatt acccagacga tccttttcat aggtaataat gccgtctgtc
     2641 cctgcaagaa tactgttcaa ggaactctcc attccagagg ttcccagcaa gctcttgctt
     2701 ccatcttcat tttcatggag ctgagctagg ccgataaaac tagaagcaaa ttgtccgttt
     2761 gggtaactac gattgggact ggttgtaaaa tcaatcccct tgacctctgc agcttccaat
     2821 tcttttttga tagacatcat attggcatag gtaatcccat ttccctttgc tccaaaggaa
     2881 acttgcttga gattaggttg cgagagttgc tctcttacat aggattcttc catgtccaga
     2941 tacttatgaa agacctctgc aaccttgtta aattgtgttt tttctacgta aagaatctta
     3001 cccgttgctg acttatagtt ctcatcaatg accgcataga cattatagga ggttgcatcc
     3061 tcagcaatcg ggactccatt tcggtcataa atagtcccac gtttggcagg aactgtacgg
     3121 gtggtttgat gaaccttctt agcttccttc gctaaatctg ttccaaagcg agtgcctgtc
     3181 ccaataatga ccgcaaaatt gactaaaaaa atggcaaaaa caaagacaga taataaactc
     3241 agactttttc caactctgcg tctgttttca gccggcgatt tccgattttt ggtcgcataa
     3301 cggattactc tttttgtcca cttcatatct tactccgcta ttctaatatt ttcattgttt
     3361 aattgcaaat cgtgtgaatt ggcaatttct ttcaaacgtt ctgcacgtaa tagttcattg
     3421 acctcttgct tggcatcgtc caattcggtc ttcttttcct ctatctgcgc attgattttt
     3481 gtcaaatcat tctgcacttg caagagcttg gtctgcataa aaataatact aatggctaca
     3541 ataagagtgg ttacagcaat ggaaaagtaa aaagcttttt ccacacgcga aaaccgttta
     3601 agttgcatct gtagtatttg acctgttttt tccatttttt ctgccatctt tttccctctt
     3661 acttgtgaat ttttctggcc acgcgcaact tggctgagtg cgagcggtta ttggcttcta
     3721 actcttccgc acttggcaag attggcttac gggacaccaa ttccatcttg ggcttgagat
     3781 catctgggat gaaaggcaag ccttttggaa cttcaactgt tgaagcttcc ttgaacaatt
     3841 gcttggtcaa gcggtcttct aaggaatgaa aggtaatcac tgaaattcta ccatccagag
     3901 ccaacatatc catagcctgc tggatggact catctgccgc tcccagttca tcattgactt
     3961 caattcgaat agcctggaaa atctgcttag caggatgacc cttcttcttg agttccttgg
     4021 caggtttgac caacttgata atctctgcta actcagtcgt tgtctcaatc ggcttcactt
     4081 cacgcgcttg ctcaatctta cgcgcaatct gtttagagaa tttatcctca ccgtatttga
     4141 agaaaatacg aaccaaatca tgatagtcat agtgattaac cacttcatag gctgtcagac
     4201 tagcatcctg attcatccgc atgtccagtg gcgcatcctt tttataagaa aaaccacgct
     4261 cacgctggtc caattgagga ctagacactc ccaagtcata acaaattcca tcaatttcct
     4321 gaacaccagc ttcgcgcaaa cgtgcctgta aatgacggaa gttatccttg ataaaggtca
     4381 ccattccctt ctcaatgtaa ggtgccaagc gtttttgcgc attgtcaatg gcattctgat
     4441 cctggtcaaa ggcatagaga tggccttttt cacttaattt acttaataaa tactcgctat
     4501 ggcctgctcc acccaaagtc gcatcaacgt agataccgtc aggttttacg tcaagcatat
     4561 caatcgtttc gtggagtaag accgttacat gatgaaattc ttttgtcata tcttatctat
     4621 tttaccacaa atccgaccag cttgcacttg tcagacaaat aggacaagga gactactatt
     4681 tcttcaaaaa atatgtcaaa tatacttgac actttatgtt taaaagacta taatatagtc
     4741 aaatatatac gacataaatc agaaaggaga ccagatgaat cgtgtgaaag aatttcgcaa
     4801 ggaattgggt atttcccagc tcgaactcgc caaggatatc ggtgtctcga gacagactat
     4861 caatatgatt gaaaacgaca agtacaatcc aaccttggaa ctctgtctca atctcgcccg
     4921 cagcctccaa actgacctca acagtctctt ttgggaggaa gatttttaaa aaaggagcaa
     4981 actcatgaaa aaagaaacct tcactgaaaa actgatcaaa cgcacatacg gtatttctgg
     5041 tccccttgac gaatacaaac ggcgtgaggc cgatagtatt gggaaccaag tctttatcgt
     5101 cctcttttat ctgatgattt tcggaaatct tattccactc cttctagcct ataaataccc
     5161 tcaagaagtg gctctaatct atcctcctct gattttagtg attgccctca tcgctgctgg
     5221 ctatgtcacc taccaaatga aaaaaacagg catcacagtc attgaaccag atatgctgaa
     5281 tgagaaagaa agcaagcaac tacactaccc aggtctgaaa gcaggtttgt tctttggtct
     5341 atggatgttt tttataactc ctcttctcag tatactcata gatgaaggtc aggactattt
     5401 tcattctctt ctcactataa gaaatggtgt atcaagtatt ctcggttcta ttttcttcgg
     5461 agcgagcata cagttcctca tctcccgtcg cattgcaaaa actaagaaaa atcaagatga
     5521 ggattaggag gaaactcatg aaaaaagaaa agaaacagtt acgttatcct ggtctgaaag
     5581 caggttcgat ttatggaaca gtgatatttt ttataattcc actcattgat accctaacaa
     5641 gtgaaaatcc aaattttatc agttctcttc taaatacaaa acatattttt aaaactatac
     5701 tgggagcttt ctttttcgga gtgatgatac atattgtcga ctcacttcgt attgcaagag
     5761 ctaaaaaaga ccaggattag gaggtgcctt atgaaatcac tagctagact actgaacatt
     5821 catgttttta tcagtatttt ccttttcttc gcccttattt caggagctgt ttctcataca
     5881 gttttactac tcctactcct ctttcttcct gcgctcaata aaggacttga gaaaatacaa
     5941 tcaaaacgga tacctgtcct caacgcagcc ctcttctttc tcctcatatc ctttccacaa
     6001 cttttaacca accctgtcca atggaaattt tcaatattcc tagtcgtaac catcatttca
     6061 agtttggcct acttctataa cttttatcaa gtagttaaag aagtagatca aaaacagttg
     6121 atttaggagc ttgctcccat aatttctcat ttcataagca aataatccca cctagcttgt
     6181 tctaggtggg attattttat ttcttaacag agattgcaag tcattttcct tcattttaga
     6241 aagtcgactc tttccaattc acactacaat ccaagacttg ttgcctttct tcttgattac
     6301 ggccttcaat ctgatcaatc aaaatctttg tagcctgttg tccttcctca aaggagggct
     6361 gaaccagcgt cgaaacactt ggagaagaaa agcaagtcca ctccgtattg tcaaaaccaa
     6421 tcaacccaac ttgtggcaag ttataattca actctttgat aacggtaaag actagaggta
     6481 gggcccaaca gttagggata aataccagag ttttttcatc gggatcgatt tctttttgta
     6541 aaaattcctt aatttgttcc aaattcgtat gcttatcttc aatggttaga ctggcgtgac
     6601 gcatattagc atctgttaaa gcatccacaa aaccacttgc ccgctcaatc cgagtactca
     6661 aacgactcgt atccgctgta atcaagagaa aatgctcata acctttttcg atacaggact
     6721 gggtcatgtc ataaacggca tcatagttat tggttttaac ccagctagtc cggtgttcat
     6781 agagctgact atcaaaaaag accattttct tctttttctc atcgatgata cgagaatatt
     6841 ttcggaaatt agaggtcggc tgaataataa agccgtctac tcccaagaga agcatgcttt
     6901 caatataccg gtcctcactc tcttggctgt aattactatt tcctatcatt acctggtagc
     6961 cattctggct ggcgatatcc tcaattccct taacaatttg gtttgagaaa ctgttggtaa
     7021 tatcaccgat caaaacaccg attaattttg ttcgtttgga gtttaagcta cgcgcaacaa
     7081 tgctcggttt gtaatttgtt tcatgaataa ctttttcaat cttttcacgt gtctcttggg
     7141 acattttttc atatttcccg tttaggtaaa atgacacggt tgttttcgag gtctgagcca
     7201 tttccgcaat gtcttttatg gtcagtttct tctccaatct aaaacacctc ctactaatac
     7261 ttattataac atattcacta ttcaaaatgc ctatttctag cactgcaagc gaatcattct
     7321 ctcatttatt ttatcatata ctagacattt tccacgcatt tttgttccgt caacgaggca
     7381 caatttttca cctacacaaa tatcctcgct taataacaga atcagcttgt ctgtttcgtc
     7441 atttttcact tcaaaagcca gagcattttc cacttcacga tcatctgtct ggtagacaga
     7501 atgacggatg atttcaaaac tctcatgcgc aatgatggta taatccagca tctgattctc
     7561 aaagaattgg cgcttggtga gtttgttact tctttcaagc tcattgtatt taggagaaat
     7621 gatggtatct tccaaatcaa aatcaacttc actccatagt ctcaactgat tgattttccc
     7681 atcttgatag gtcacatcct tgtcaaggat aaactgagtc aacgcctcat gctgaccttg
     7741 acacctgatg tcatccacca agagccagac atcctctacc aacatgagga tttttctctt
     7801 gtgaagataa ggcaaatcag gttctgctga ccaataagcc ccttcaatat aatgcactcc
     7861 ctccctttct ttatggtgac aaaacaggga gtgaggatag tattcatatt cccaggatcc
     7921 cgtgattctt tccggagctt tcccatctac aatgcaggtc gaatgactcc aagcactctt
     7981 taagagataa cgttcatata tctcccgata agaataacgc ccagcatcta tgaaaatagg
     8041 ttgtccttga tactgtaagc aaaaactatt ctcgtcacta tggctatggg cacttcctag
     8101 cggaccattt ttgaaaaata gataacgatg ttcatcctta atgcagacat gtccagagtc
     8161 ttcaaagatc atggacttag gctgccaagc tctcttttca aattcctgca gtcgcttgac
     8221 cttttctcgc cccaggaaca agaggctaag caaatcgact ttaacatcca gaccgttaag
     8281 aaggtcttcc ttgttcaaaa ccacagcaga caggctcaaa atttctgtcg tttctgtaga
     8341 atcgctatca ccaaaagcca aagttcgtcc atctaagcct gtcatcattt gaatataggt
     8401 cgccatcttt tccagcaact ctcggtaact atcttgcaag tctggaagca agagacacaa
     8461 atccagcaag gctttataaa cctctacatg atagagaatc gactgttcaa actggcttcc
     8521 atctcctaaa atctgtgtct caatttgctg tttcaactcc tctgaagcaa aatggtaagc
     8581 ttcttctagg tccatcttat ctgaaaagaa ataatagata gcaagcatcg gaattgtttg
     8641 taaaatcccc cagttactaa gggtgtactt ggcgcgatag tagcttttca taaagtcaat
     8701 ctgcttttct agactgacca aaattttctc tagttctttc tcctctagca tgtcaaattt
     8761 caagaggagc aagagtagtt tcaaccaagt aaaggaacga atacccgtat ccaaggttct
     8821 agtcatcaag gattgaggag aaaattctct cacctgctca atccaatcaa atagaaagaa
     8881 cttgcacttt tgaatatagt ccttatctcc ttctaccaca taccctatca taaactgcaa
     8941 gagatattct tgtcgattga gcatataagc ccattctgga tcatcttcaa atacttgatc
     9001 ccataccatc ggctggattt gatggatttt tgaacaaggc tccatatccc aaggactatc
     9061 aaacataaaa cgattgtcca tcaagcgttc aagggaactc ttgactttct catagtcttt
     9121 tgaacagtgc gacaagatat aatcacgaca ttggtttcca tcgactcttt caaaaaattg
     9181 tcttctttct tctttcatta tctattacca gaaaaagaac tacttaaaaa gcagttcttt
     9241 tgtctttccc attacacttt ccttttctac atagatgacc acaccttttg caatctgcaa
     9301 ggagaccaag tcatctttga tagaaatgat ttttccatga attccagaca ataacaacac
     9361 ttcatcacca gatgttaaag aagctaaata ctcttgtcgt tgctccgtct gtttgcgaag
     9421 caacttttgc tgacgaatag aatgaaagct tgacagtaaa aggggactca ctgccaagac
     9481 aatcactatt ccataaaaca atgttgtatc cattaagcta taatcttaag ccagcttccg
     9541 ataattccga tgacaactgt taaaataacg agtttatatg ttgtccattt cttttctttg
     9601 atcaagtagt aaactaaaag tgtaaatagg gctggtagaa gagctggagc aaccttatca
     9661 agcattccct gaatacttac gatactttgt ttagcgtctg ctttaacttc ccctgcagca
     9721 aaggtaatcg gcaccataat cttaacagat gtcgctgcca aaccagcaat tacggttaca
     9781 ccgataatat tggcaatacg agaaatcgtt gccatctgtt cgcttagttt atcaatcaca
     9841 gttgttccta gtttgtatcc atacagacca gttgacaatt taatcgctgt taaaatcgta
     9901 ttcatcgcaa ggaagaacaa gattggaccg acaaccaagc cttcttgagc aaacgaagct
     9961 gcgatggttg agaacaatgg agctaaacag aattgagaaa gagaatcccc aatacctgcc
    10021 aatggtccca tcaaggccat cttgatgcta cgtgtttctt ttgccggacg gccattttcc
    10081 aacattacaa gatgcaagct ggtaataaaa ggcaggaagt gtgggttggt attatagaat
    10141 tcacagtttt cttccaaggc ttggtagaaa ccttcctgat cctctccata gtgttttttc
    10201 aaagcaggat acatcacatt ggcatatccc aacccttgat agttactata gttaaatcca
    10261 ttttgacaaa agaatgcccg caaagacgtt ttaagataat cacgttttgt taatttgtta
    10321 gatccagtca tcgtgtgctt cctcctctac cacatgattc gctgtttttg gcttgttata
    10381 aaattcaatc aaagcaaaga tagtacctac aattgcaata ccaattgttg ggatgtttag
    10441 ataagctgca caaacatatc ccaacaagac aaagggaatc aactctttct tagccatcac
    10501 tgacaagatc atcgcaaaac cgatagctgg gagcatttta ccagcaactg tcaaacctgt
    10561 aagtaatacc ggtggaatgt agtctacgag tttcaacaag gtatccattg aaagggcacc
    10621 caagcaaccc aaggtaaatc caataaaggc aaacaaccaa attgttgcat ttagagtaaa
    10681 cttgaatttc ttcaaattat ggtttttcaa gtgcttcatc gccgtttcag gcgcaccagc
    10741 acgtacagtg taggcgaaag tttgtaagaa ttgaatcgcc acagcaatcg gagtagagag
    10801 ggcaagagcc gcttctggac tgactttacc agcactagtg atagccatca aggtaccaaa
    10861 gataccaggt ccgattgggt ttggtggaac agtacctcca gcaccaacac cgaatcccat
    10921 ataagccaat tcaccaatag ctcccattgc aagagcagta ggcagatcac ctagaataat
    10981 tccgacacca aatgacagaa caatacatct attggtgtaa attcctaaca gcattccact
    11041 aaaacagaaa gctgtccata atccaattaa aatcgcttga aatacattaa tcgacataac
    11101 gatctcctct tttaaatata gtccataatg ttgacttgaa cagctccatc atttcctgtt
    11161 ggagttgttt tcgtattaaa tgttacttga tgagtttggc ttaattcctt gagggctgcc
    11221 ttgtcctctt cacccaggaa gatggagcgt gtcacttgct ctttaccagg ggcattgtga
    11281 atgttcccaa tattgatttc tttgataggg acaccacctt ctaccaaggt taaagcgtcc
    11341 tttacatcct ttacaacgat aaagatcgtt tgagcaggat tagccttgtg aatgatatca
    11401 atcacctttt gcaaagggaa gaaacgcatg gcaactgagt ctggcacaac tgttttcatc
    11461 agagtttgtt gcatcttgtc cgtgcttact tcgtcattgg caacaatgac cgtattacaa
    11521 gctaggtatt ttacccaaag ttgtccttgt ccatgaatca accgttcatc gatacgggtc
    11581 ataataatat ttggcattgt catatttctc ctcctaccaa tataggttcc agtctttgta
    11641 gaaacggata agggcttcta ggtaatagta gtcaccccag atattgcctt catccactcc
    11701 tttacctgaa tgccatgagt acacaccgtg gagaagactt gtcccaccag gggtaaattg
    11761 atcatttgca taatgttcga tcaaggaacg aagcatggca tgcatagcat gtttataaat
    11821 atctttgtca gcatccacct ctgggagatg ttttagcatt tcatgaatcc cacagacggc
    11881 gatagctgtt gctgaagaat ctcgtgattg agcactacca tcattaaaaa tcaaatccca
    11941 ataggacaca tgatcttttg gcagacgatt caagaaataa ttggtcacac ccttaaacaa
    12001 gtcaaagcag gactcgtctt ttaagtgacg ataagtcaaa ggaataccat agactcccca
    12061 tgattgacca cgtgcccagc atgaatcatc actataccct tgtctcgtta cacctttaaa
    12121 gggttgacct gtctcaggat caaaatagaa ggtgtggaag gacgaagcgt catcacggat
    12181 tacattatta gctgaagcat agaaatgact ttctgcaata tcgtagtatt tttgatcgcc
    12241 tgtttcttga taagcaaaga ataagagttg gatattgagc aagcagtcga taatcaaacg
    12301 gtaatgctct ttcttgccca agtctcccca agcttgaata aaaccacctt tttcttgata
    12361 gcgttcaatc aacttatctg cagctttcaa ggttgcttct ctagcctctc catctccatt
    12421 tatcttatat tcagccatac aagacggtgt gtacaagaag ccgagatcat ggtgatccaa
    12481 ttctactctc ttattgacac gatccaggaa agaaagaaca tttttatgag cgatgttttt
    12541 aaatgcatcc tgttgactgt attcataagc caaccacagt tctcctgtcc agaaaccatt
    12601 ggtccattcc gtgttatcca tgattggata gacattatca aaggtagctg gcgtcgggaa
    12661 atcttccttg aaatagtcaa ggttgagttc taactgccga ataaccttat cgattgcctg
    12721 gccgacttct tctttcgtca gaagtggtac ttctaagaag cgctcaggcg attttatttt
    12781 ttcaatcgta acctttttta tcattctttt accctttaaa tacccgattc gaaatcttct
    12841 tcctctgcat ccgttgaaaa caattcttta ccatttacga ctccgccctg ggccgccact
    12901 actgatttat taacaacctc atcaaagcta tgagccattc tagcaaagac tgcttccatt
    12961 aacatggcta agttcaaacc cgagagaaca ttcattgttt tgactggatt ttctcccatt
    13021 atggtagaag aaaccttgaa tggcgatcct cccaagagat cacttaggat taaaacttct
    13081 tcttcatttg aaattgcaag taagattttt tgcttgagtt catctgctga cattccttcc
    13141 acaaagtcaa tcgcctccac attttcttga ttacctgcaa tcaattgtaa agaactataa
    13201 atccctgtag caaaatgtcc atgccctaca agtacaattt tcatcttttc tcctttcttg
    13261 tttaggttta ccggtttact ttatattccc cattgtaatc ctttattttt atcttgtcaa
    13321 tagttttttt aaaaaaatat tagcgctttc attatctttt taaccatttt aataaacaga
    13381 aatttcaaaa cattccgttt ttcaggaaaa caaatctttt tataaataaa aatatttatt
    13441 tattgacaaa accggtttgc tagaatataa tttggttata gagaagtctt aacaaatttg
    13501 aaaggaagta tcaaatgaca aatacatcat tctcaattga gcagttttct ttaaaaggaa
    13561 aaattgctct catcaccggc gcttcttatg gaattggatt tgctattgcc aaatcctacg
    13621 ctgaagccgg cgctactatt gtctttaacg atatcaatca agatctggtc aataaaggga
    13681 ttgaagctta tcgtgaagtt ggcatcgaag cccatggata tgtctgtgac gtgacagacg
    13741 aggacggtat ccaagccatg gtcaagcaaa tcgaacaaga ggttggtgtc attgacatcc
    13801 tcgttaataa cgctggtatt atccgccgag ttccaatgtg cgaaatgagc gccgctgatt
    13861 tccgtaaggt catcgatatt gacttaaacg caccatttat cgtttcaaag gcagttattc
    13921 cttctatgat aaagaaaggg catggaaaga ttatcaatat ttgttcgatg atgagcgaac
    13981 tgggacgtga aacagttagc gcttatgctg ctgctaaagg gggcttgaaa atgttgaccc
    14041 gcaacattgc gtctgaatac ggtggagcca atatccaatg taacggaatt ggaccgggtt
    14101 atattgccac tcctcaaaca gcacctcttc gtgagttgca agaagatggt tctcgccacc
    14161 catttgacca gttcatcatt gcaaaaacac ctgctgcacg ttggggaaat cctgaagatt
    14221 tgatgggccc tgctgtcttt ctcgctagtg atgccagcaa ttttgtcaat ggccacatcc
    14281 tatatgtaga tggcggtatc ttagcctaca tcggaaaaca acctgagtaa aaatagaaag
    14341 aagatcttat gaaaatcgca ttaatcaatg aaaatagtca agctagcaag aatcacatta
    14401 tttacgatag tctaaaagaa gcgacagata aaaaagacta ccaattattt aactatggta
    14461 tgcgtggaga agaaggagaa agtcaattaa cttatgtgca gaacggacta atggctgcca
    14521 tccttttaaa tacaaaggca gttgactttg ttgttaccgg ctgtggtacg ggtgtagggg
    14581 ctatgcttgc tttaaacagc ttccctggtg ttgtctgtgg tctagcagtg gacccaactg
    14641 acgcttacct ttattctcaa atcaatggtg gtaacgcctt gtctatccct tatgccaaag
    14701 gatttggctg gggggcagaa ctgaccctca aattgatgtt tgaacgctta tttgctgaag
    14761 aaatgggcgg tggctaccca agagaacgtg taatccctga acaacgcaac gctcgtatct
    14821 taaacgaggt gaaacaaatc acccacaatg atttgatgac catccttaaa acaatcgacc
    14881 aagacttcct caaagacacc atctctggca aatacttcca agaatacttc tttgaaaact
    14941 gccaagatga tgaagtcgct gcttatttga aagaagtatt agccaagtaa agctattcta
    15001 aaccagaaag gaactaatgg atgacgaaaa tattactgtt tggcgaacca ttaattcgaa
    15061 tctcaccatt agatgccacc agtatcggcg atcatgttgc cagttcgact tattttggcg
    15121 gatcagaaat taacatcgct tgtaatttgc aagccctggg tatctcaacg aaagtcttta
    15181 ccgcactccc tgccaacgag attggagatc gttttctcac attcttgaaa cagcaccaaa
    15241 tcgataccag ttcaatctgt cggcttggcg atcgaatcgg cctctactat ttggagaacg
    15301 gctttggttg tcgtcaaagt gaagttttct acgatcgtaa gcatacgagt atcagccaga
    15361 ttcggccaaa catgctagat atggattctc tctttcaggg gattagccat tttcatttta
    15421 gtggaatcac catagctatc ggtcaagagg tccgtgcgat ccttctccta ctcttggaag
    15481 aagccaagcg ccgaggaatt gtcgtttcaa tggatctcaa tctgagaaca aagatgattt
    15541 cagtcctaga agccaagtat gaattttcta agtttgcacg ttttactgac tattgcttcg
    15601 gtattgatcc tctcatgatt gatgaccaaa atctagagat gtttccaaga gacagtgcta
    15661 gcctagaaga ggtggaaaat cgcatgcgac ttttaaaaga agcctatggt ttcaaggcca
    15721 ttttccatac cctccgatct agtgatgagc aagacaaaaa tgtctatcaa gcctatgctc
    15781 tggaagaacg atttgaagag tctgtccaac taaaaactgc agtctatcaa cgaattggta
    15841 gcggggatgc ctttatatct ggtgcccttt accaactact ccatcattcc tccctaaaaa
    15901 ctaccattga ctttgcagtt gcgagcgcaa ctctcaaatg cactcttcca ggagaccatc
    15961 tctccacttc cgcaactagt attgaaaatt tactggcaaa tgcacaagat atcattcgtt
    16021 aggagaatta catgaccaaa tcagatacga ttattgaact aaaaaaacag aaaattgtcg
    16081 ctgtcattcg aggaaataca aaggaagaag gactacaagc ctcgattgct tgtatcaagg
    16141 gcggtatcaa agctattgaa atcgcctata ccaatcagta tgcaggacaa atcatcaagg
    16201 aacttgtaga cttgtatcag gacgatcaga gtgtttgtat cggtgcaggt actgtgcttg
    16261 atgccgtaac tgctagagat gccattctag ctggagcaaa ttacgttgtt tctccatctt
    16321 tccatgctga aactgcgaaa atgtgcaatc tctacagcac accgtacatt ccaggctgta
    16381 ttaccctcac agagatcacg actgcacttg aagccggtag tgaaatcatc aaactcttcc
    16441 caggtagtac tctcagtcca gcatatatct ctgcagtcaa ggcaccgatc ccacaagttt
    16501 ccgtaatggt aaccggagga gtcggcctaa acaacatccc tcaatggttc gctgctggtg
    16561 cagatgccgt tggaattggt ggcgaactca ataaactcgc ttcccaaggc aactttgacc
    16621 gcatcagcga gattgcccaa cagtatatta cactcagata aaatcataac tacccgtcta
    16681 acgggtggtt tatcccagag ctataagccc aaatcatcag ccagcgccta aagacgctgg
    16741 cttttacgtt gttcaagcct tattgctctt gactcgtcac ttgcctcttt aagagacttt
    16801 ggtattactt accactatcc ctaaagggat cctcatattc ttttacactc aatttatcta
    16861 gtgctatagt agattgaaac tggaatagta
//
LOCUS       DAVBHS010000032        16647 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVBHS010000032 DAVBHS010000000
VERSION     DAVBHS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16647
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.032"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            63..527
                     /locus_tag="VQ651_000758"
     CDS             63..527
                     /locus_tag="VQ651_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HET2753781.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            685..1110
                     /gene="lacA"
                     /locus_tag="VQ651_000759"
     CDS             685..1110
                     /gene="lacA"
                     /locus_tag="VQ651_000759"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HET2753782.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            1141..1656
                     /gene="lacB"
                     /locus_tag="VQ651_000760"
     CDS             1141..1656
                     /gene="lacB"
                     /locus_tag="VQ651_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HET2753783.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            1667..2596
                     /locus_tag="VQ651_000761"
     CDS             1667..2596
                     /locus_tag="VQ651_000761"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HET2753784.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDDQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKLVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLEELKEVLQESLFTGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAGLLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            2598..3578
                     /gene="lacD"
                     /locus_tag="VQ651_000762"
     CDS             2598..3578
                     /gene="lacD"
                     /locus_tag="VQ651_000762"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HET2753785.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKILVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3812..4648
                     /locus_tag="VQ651_000763"
     CDS             3812..4648
                     /locus_tag="VQ651_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2753786.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALIQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4684..5001
                     /locus_tag="VQ651_000764"
     CDS             4684..5001
                     /locus_tag="VQ651_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2753787.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            5003..6697
                     /locus_tag="VQ651_000765"
     CDS             5003..6697
                     /locus_tag="VQ651_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2753788.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERSGKSNDELKEKVAANFNTAKADAILEKA
                     GVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            6906..8312
                     /gene="lacG"
                     /locus_tag="VQ651_000766"
     CDS             6906..8312
                     /gene="lacG"
                     /locus_tag="VQ651_000766"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET2753789.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPLDPKNPSDVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            8355..8537
                     /locus_tag="VQ651_000767"
     CDS             8355..8537
                     /locus_tag="VQ651_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HET2753790.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLFLNFINPEE
                     WDTISFDSDFMPFQQS"
     gene            complement(8815..9576)
                     /locus_tag="VQ651_000768"
     CDS             complement(8815..9576)
                     /locus_tag="VQ651_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2753791.1"
                     /translation="MLKQEKLAKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(9758..10720)
                     /gene="nrdF"
                     /locus_tag="VQ651_000769"
     CDS             complement(9758..10720)
                     /gene="nrdF"
                     /locus_tag="VQ651_000769"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HET2753792.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(10908..13067)
                     /gene="nrdE"
                     /locus_tag="VQ651_000770"
     CDS             complement(10908..13067)
                     /gene="nrdE"
                     /locus_tag="VQ651_000770"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HET2753793.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(13148..13366)
                     /gene="nrdH"
                     /locus_tag="VQ651_000771"
     CDS             complement(13148..13366)
                     /gene="nrdH"
                     /locus_tag="VQ651_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HET2753794.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            13717..13980
                     /locus_tag="VQ651_000772"
     CDS             13717..13980
                     /locus_tag="VQ651_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HET2753795.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            13986..15719
                     /gene="ptsP"
                     /locus_tag="VQ651_000773"
     CDS             13986..15719
                     /gene="ptsP"
                     /locus_tag="VQ651_000773"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET2753796.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            15974..>16647
                     /gene="phtA"
                     /locus_tag="VQ651_000774"
     CDS             15974..>16647
                     /gene="phtA"
                     /locus_tag="VQ651_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HET2753797.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASE"
BASE COUNT         5221 a         2918 c         3460 g         5048 t
ORIGIN      
        1 aactagaatt ctagttttgc aggaagtcta ataaagaaac tagcaaggtc aggccttact
       61 ttattggtgg acatccagga tttaattgtc ttctacttga tgatgaagtc tatgaagatt
      121 attatctaga gtttgagaaa gaagagattt gctctgttcc acgtcctttc ccagaaacag
      181 gtatgttgga tttccaagat agaagtccat ggctagaggg acaaaaagaa atagatctta
      241 gttatgatct tttcagcaca gatgcagtga ctttggatga attgcaatct cgaacaattg
      301 cccttcgttc tcttaaacat gataagggat tgaaagtgca ttttgcagaa tttccaaacc
      361 tcatcatctg gtcaactttg aacaagggac ctttcattac ctttgaacca tggtctggct
      421 tgtcaacatt ccttgaagaa ggagatcatt tagaagataa gaagaatgtt tgtctcttag
      481 aagccaatca ggttgaagaa ttagggtttg agatagaagt tttataaaaa aagtaaaaaa
      541 taaataacaa aaacaaacac aaactgttga cttcttcaaa caatagtagt atattatgtt
      601 tgtgaggact aattgactgt tttttaaaga cagccaatac actgctggaa tccagcatag
      661 aaaaaataaa aaggagtata caatatgtct attgttatcg gtgcagatgc tgcaggtttg
      721 agattgaaag aagttgtgaa agacttctta gaaaaagaaa acttccacct tgtggatgtt
      781 acagctgaag gtcaagactt tgttgatgtg actctcgctg ttgctgcaga agtaaacaaa
      841 gaagaacaaa accttggtat cgtgattgat gcttatggag ctggtccatt tatggttgca
      901 actaagatca aaggaatggt tgctgcagaa gtatctgacg aacgttcagc ttatatgact
      961 cgtggccaca acaactcacg tatgatcact atgggggcac aacttgttgg tgatgaattg
     1021 gctaaaaaca tcgctaaagg atttgttaat ggtaaatacg acggtggacg tcatcaaatc
     1081 cgggttgaca tgttgaacaa aatgggctaa ttagattaca gtaagaaagg taagttaaaa
     1141 atgagaattg caattggatg cgaccacatc gtaacagatg aaaaaatggc ggtttcagaa
     1201 tttttgaaat caaaaggata tgaagtcatt gactttggta catatgacca tacacggact
     1261 cactacccaa tctttggtaa aaaagtaggg gaagctgtaa ctagcggcca agctgatctt
     1321 ggggtatgta tctgtggtac tggtgttggt atcaacaacg ctgtaaataa agttccaggt
     1381 gttcgttctg ccttggttcg tgatatgaca acagcccttt atgctaaaga acaattgaac
     1441 gctaacgtta tcggctttgg tggtaagatt actggtgaat tgcttatgtg tgatatcatc
     1501 gaagctttca ttcatgctga atacaaacca tctgaagaaa acaagaaatt gattgcgaaa
     1561 attgaacacc ttgaaagtca caatgctcaa caaacagacg caaacttctt tacagaattc
     1621 cttgaaaaat gggatcgcgg agaataccac gactaagagg tgacctatga ttttaacagt
     1681 cacaatgaac ccatccattg atatttccta tcccttggat gagttgaaga ttgatactgt
     1741 caatcgtgtg gtggatgtaa ccaaaacggc tggtggtaag ggacttaatg ttacccgagt
     1801 actttcagaa tttggtgatt ctgttcttgc tactggttta gtgggtggca aacttggtga
     1861 gtttttggtt gaacatatcg atgatcaagt aaagaaagat ttcttttcta ttcagggaga
     1921 aactcgtaac tgtatcgcta ttctccacgg tgacaaccaa acagaagttc ttgaaaaagg
     1981 tcctgaagta ttggaacagg aaggtcaaga ctttttggaa catttcaaaa aactcttgga
     2041 gtcagttgaa gtagtagcca tctcaggtag tctgccagct ggccttccag ttgattacta
     2101 tgcgagcttg gtagagcttg ctaatcaagc tggcaagcta gtagtcttgg actgctctgg
     2161 tgcagcactt caggcggttc ttgaatcacc ccataaaccg acagtaatca aacccaataa
     2221 tgaggaattg tctcagcttc taggaagaga agtttctgag gatttggagg aattaaaaga
     2281 agtccttcaa gaatctttgt ttacagggat tgaatggatt atcgtttcac ttggtgccaa
     2341 cggtactttt gccaaacatg gtgacacttt ctacaaggta gatattccta gaattcaggt
     2401 ggtaaatcct gttggatctg gagactctac tgtggcagga atttcttcag gacttcttca
     2461 caaagaatcg gatgcaggat tactcatcaa ggcaaatgtc cttggtatgc tcaatgctca
     2521 agaaaaaatg actggtcatg tcaacatggc caactatcaa gctctatatg atcaattaat
     2581 agtaaaagag gtataaaatg gctttaacag aacaaaaacg tgtacgctta gaaaaacttt
     2641 ctgatgaaaa tggtatcatc tcagctcttg catttgacca acgtggtgct ttgaaacgcc
     2701 tcatggctca acaccaaaca gaagaaccaa ctgtggccca aatggaagaa cttaaaatct
     2761 tggtagcaga tgaattgact aaatacgctt catctatgct tcttgaccct gagtatggac
     2821 ttccagcaac taaggctctt gatgaaaaag ctggtcttct ccttgcttat gaaaaaacag
     2881 gttatgacac aacaagcaca aaacgcttgc cagactgctt ggatgtttgg tctgcaaaac
     2941 gtattaaaga agaaggtgca gatgcagtta aattcttgct ttactatgat gtagatagct
     3001 cagacgaact caatcaagaa aaacaagcct acatcgaacg catcggttct gagtgtgtgg
     3061 ctgaagatat cccattcttc cttgaaatcc ttgcttacga tgaaaaaatt gcggacgcag
     3121 gttctgtaga atacgctaaa gtaaaaccac acaaagttat cggtgctatg aaagtctttt
     3181 cagacccacg ttttaacatt gatgtcttga aagttgaagt tcctgttaac attaaatatg
     3241 ttgaaggctt cgctgagggt gaagtggttt acacacgtga agaagcagca gccttcttca
     3301 aagcgcaaga tgaagcaacg aacttgccat acatctactt gagtgctggt gtatcagcta
     3361 aactcttcca agatactctt gtatttgctc atgaatcagg tgcaaacttt aacggagttc
     3421 tttgtggtcg tgctacatgg gcagggtcag ttgaagctta catcaaagat ggtgaagcag
     3481 cagctcgcga atggcttcgc acaactggat ttgaaaacat tgatgagctc aataaagttc
     3541 ttcaaacaac agcgacttca tggaaagaac gtgtgtaaga aagtcctcct agtttaggaa
     3601 catgaatcta aaaaaattta aaaaaagttg tatgtaaagg tttacaaaat aacttacttg
     3661 tgctatactt aaatcacaag ttaatacaag gtgagtgtta ctaagtaata ttaggcatga
     3721 tcacaggtga attagaaatc agctgatttt ctagttcatt tgtggtcatt ttttgtactc
     3781 atataccttt aagatataaa aggaggttga catgtatcga attctaaatc caatgaatca
     3841 caatgtctcg cttgtcagaa atgataaggg agaagaggtg attgtaattg gtaaggggat
     3901 tgcattcgga aagaagaagg gggatttgat tgctgaaaat catgttgaga aaatctttcg
     3961 gatgaagacc gaagagtcca gagaaaactt tatggctctt ctcaaagatg ttccgcttga
     4021 ttttatcaca gtgacctatg aaatcattga taagctatca aagaaatatc attatccgat
     4081 tcaagagtat ctctatgtaa ccttgacaga tcatatttac tgttcttatc aagctctaat
     4141 tcaaggaagg tacaaggata gtaatctgcc agatatttct gctaagtatc ctgtcgcttt
     4201 tcaaatcgca aatgaagctt ttgaaattta ccgtcagaag ctagcagatc attttcctga
     4261 ggacgaaatt attcggattg cttatcattt cattaatgct gaaggtgaaa atgaagtgga
     4321 acttgtggag tcgattgata agaggaaaga aattctcagg aatgttgaag aagttttaac
     4381 ggactatgca attcaacgaa ctaaaaagaa taaccatttc tatgatcgct ttatgatcca
     4441 tttgaattat ttcttggatt atttagacag atctagagat gataaccaat cacttctgga
     4501 tatggaagat catattaaac aatcctatcc aaaagccttc gagattggtt ccaagatcta
     4561 tgatgtgatt acgcaacata cgggtcttga tttgtataaa agtgaacgag tttatctagt
     4621 tctacatatc caacgtttat tgtcataaaa atttatttaa aactatataa ggagaattct
     4681 atcatgaata gagaagaagt aacattgtta ggttttgaaa tcgtagccta tgctggcgat
     4741 gctcgttcaa aactattgga agccttgaag gctgctgaag ctggtgattt tgaaaaagcg
     4801 gacgctctgg tagaggaagc tggtagctgt attgcagagg ctcaccacgc gcaaacaagt
     4861 ctattgacta aggaagcttc tggtgaggac ttagcgtata gtgtgaccat gatgcatggt
     4921 caagatcact tgatgacaac tatcttgtta aaagacctaa tgcaccattt aattgaactt
     4981 tacaagagag gagttaaata aaatggataa gttaataatt tttattgaga aaggaaagcc
     5041 tttctttgag aaattatcga gaaatatata cttaagagcg ataaaagatg gttttatttc
     5101 cagtatgcca gcagtattat tctcaagtat ttttatacta atagctgctg tgcccaatat
     5161 ttttgggttt aaatggtctg atgaacagtt agcttttatt ttgaaacctt acaactactc
     5221 aatggggatt ttagctctct tagtagctgg gacaactgca aaatcgttaa cagattcagt
     5281 taatacacgt agtatggaga aaaccaacca aatcaactac atgtcaactt ttttagcagc
     5341 tgttgttgga ttgttaatac tggcagctga tccaattgaa ggtggatttg ctaatggtct
     5401 tttaggtact agaggattac ttactgcatt tttggcagct tttattacag taaatatcta
     5461 taaagtatgt attaaaaata atgtaaccat tagacttcct gaagaggttc caccaaatat
     5521 tgcacaagtt tttaaagatg ttattccgtt tgccttatct gttctttcaa tatatggatt
     5581 ggatttaatt gttagaaata tttttggtac aaatgtagca gaatctgttg gtaaaatatt
     5641 agcccctcta ttttcagcta ctgatggtta tatcggttta gctattgtat ttggtgctta
     5701 tgctttcttt tggtttgttg gaattcatgg gccatctgtt gtagaacctt taattgtagc
     5761 tatcagttat gcaaatatag aagctaatgt tcagcttgtg caagctggta tgcacgcaga
     5821 taaaatttta aatccagtta ctcaaacttt tgttgtaaca atgggtggta ctggtgcaac
     5881 actagtagtt ccatttatgt ttatgtggtt atgtaaatca aaaagaaata gaattgtcgg
     5941 acgtgcgtct gtagttccta ctttctttgg cgtaaatgaa ccaatcctat ttggtgctcc
     6001 aattgtttta aatcctatct tctttattcc gtttgttact gctcctatta tcaatgtttg
     6061 gattatgaaa ttttttgtag atgttctaca aatgaatagt ttcagtatta ttttaccttg
     6121 gacaacacct gctccaattg gtattgttat gggaacggcg ctggctccat tgtcattcgt
     6181 cttggctata actttaatca ttatcgacac tttaatctat tatccatttg ttaaagttta
     6241 tgaccatcaa attttagaag aagaacgttc aggtaagtct aatgatgaat tgaaagaaaa
     6301 agttgctgca aacttcaaca ctgcaaaagc ggatgctatt cttgaaaaag cgggtgtcga
     6361 tgcagcacaa aataccatca ctgaagaaac aaatgtcctc gttctctgtg caggtggagg
     6421 aacaagtggt ctccttgcaa atgctttgaa taaggcagca gcagaataca atgtccctgt
     6481 gaaagcagca gcaggcggct atggtgctca ccgtgaaatg ttaccagagt ttgatcttgt
     6541 tatccttgcc cctcaagttg cttcaaactt tgaagatatg aaagcagaaa cagataagct
     6601 cggtattaaa ctagcgaaaa cagaaggcgc tcaatacatc aaattaactc gtgatggaaa
     6661 aggtgctctt gcattcgtac aagcgcaatt cgattaaggc tagagactct gaaatagtct
     6721 cctatcgtta cggaaatcgc tatggcgaat ttcctattat taattcgtcg gtaaaaagat
     6781 atcgttttta cctcctcatg tcacaattcg gtgacttggt acaagaagtg agatggagaa
     6841 ggatggctca ctgactcctc tcctctcact tttactttat ttaaatcaag aaataggtga
     6901 aaaaaatgac aaaaacactt ccaaaagact ttatttttgg tggcgcaaca gctgcctatc
     6961 aagcagaagg tgctacacat actgatggta aaggaccagt agcgtgggat aaatatcttg
     7021 aagataacta ctggtacact gcagaaccag ccagtgattt ttacaatcga tatccagttg
     7081 acctcaagct agcagaagag tatggtgtca atggtattcg aatttctatt gcttggtcac
     7141 gtattttccc gactggttac ggccaagtaa atgctaaagg tgttgagttt tatcataatt
     7201 tatttgcaga gtgtcacaaa cgtcatgttg agccctttgt aactcttcat cactttgaca
     7261 cgccagaagc tctccactca aatggagact tcttaaaccg tgaaaatatc gaacattttg
     7321 tagactacgc tgccttctgt tttgaagaat ttccagaagt aaactattgg acaaccttta
     7381 atgaaattgg gccaattggt gatggtcaat acttggttgg gaaattccct ccaggtattc
     7441 agtacgacct tgccaaagtc ttccaatcgc accacaatat gatggtgtct catgcgcgtg
     7501 cagttaagct atataaagat aaaggctata aaggcgaaat tggtgttgtt cacgctctgc
     7561 caactaagta tcctttagat cctaaaaatc cgtcagatgt tcgtgcagct gagttggaag
     7621 atattattca caataaattt atcttggatg caacttatct aggtcgctat tcagctgaaa
     7681 cgatggaagg tgtcaaccat atcttatcag tcaatggtgg tagcttggat cttcgtgaag
     7741 aagattttac agcattagaa gcagcaaaag acttgaatga tttccttgga atcaactact
     7801 atatgagtga ctggatggaa gcctttgatg gagaaactga aattatccat aatggtaagg
     7861 gtgagaaagg aagttctaaa taccaaatca aaggtgttgg tcgtcgtgta gctcctgatt
     7921 atgtaccacg tacggattgg gattggatta tctaccctca aggtttatat gaccaaatta
     7981 tgcgtgtgaa gaaagattat cctaactaca agaagattta catcactgaa aatggtctcg
     8041 gctataaaga tgagttcgtc gataacactg tttatgatga tggacgtatt gattatgtga
     8101 aacaacactt ggaggtcttg tctgatgcga ttgcagatgg agcgaatgta aaaggttact
     8161 tcatttggtc attgatggac gtcttttcat ggtcaaatgg ttatgaaaaa cgttacggtc
     8221 tcttctatgt agactttgaa actcaagaac gttatccgaa gaaatcagct cactggtaca
     8281 agaaagtagc ggaaactcag attatagact agtagaatta gtcattagat atagaatttt
     8341 agtgagtcaa aaagatgttc aaagatttta tccaatctat ttatgaaaaa gtttatatta
     8401 taaatttcga aaaatgctct caaataccgt gtttgacgag tgaagaattg aaaagtctat
     8461 ttttaaattt tatcaatcct gaagaatggg atactatctc ctttgattca gattttatgc
     8521 catttcaaca atcgtaacca atttctcaaa aaagttaaat cttatattta gtactctgca
     8581 aaactcttat ctaatcacgt tgcttatact caatgaaaat caaagagcaa ctttaaacta
     8641 ggaagcgagt cgcagatttc tcaatgcata gctttgagga attgggcaaa aagtctttga
     8701 tatagaaaaa gcatagtatc aggtgtttca acacctgata ctatgctttt tattgtggga
     8761 agatttactt tttttcttct gaaattgagt tgttacccag gctctttcag tttattaagg
     8821 cttgatgact ttaatgtgtt tagatagctt aaaaaggatt gaatcactta gtttagaatc
     8881 tgaaacaata gtatcaagat ttgatacatt ataaaaagta taaaaatcaa acttattgaa
     8941 cttactatga tctgcgagta aatatttttt attagaatta tttaaagcga tgcgttgagc
     9001 ctctccctct tcctcgctaa aagtagctag agctccgttt tgaataccat tacagctaac
     9061 gaaagcttta gaaaattgga gattagagag attttgtagg gtcaatgtgc caacaaaagc
     9121 acctgtaata tcgcgataat ttccacctat caaaatcaaa tctgttaatt ttcgttcgct
     9181 taaaatcaga aaaacaggta gactgttggt tacgacgcgg atattgtcaa taggcaactc
     9241 acgcgcaaaa aactctaatg ttgttcctgg tccaatgaaa atagtttctc tttcttctac
     9301 tagactgcct gcaaaatggg ctatttcttg tttttctgcc gtttggaggg cttgtttttc
     9361 aatatttgat cgctcattag tcaaaaggga gttggttcga agtttttcag ctccaccatg
     9421 cacacgaatc agcaaatctt tatcagctaa ttcctgtaaa tagcgccttg cagtcatatc
     9481 tgaaacggct atttcgtcca taatctgttt aactgttatg gttcctttac tatttactat
     9541 ctctaaaatt ttggctaatt tttcttgttt gagcatatta tcacctcgtt tcctactact
     9601 atcttaccat aaacaaactc atcattcaaa tacaaaaaca acaaaatgaa acaaaaacaa
     9661 aaatatcgaa gtttgttttc aaaactttcg atatttttgt tgggttataa ctttgatgtt
     9721 tctagtttac tttttgatga ttgagagtga tggagaatta gtctaaaccg tagttatagt
     9781 catcgtcttg catggcttca acttcgccaa gaaggtaacc atttccgact tgagagaaga
     9841 agtcatggtt ggaagttcct gttgaaatac cgttcataac gattgggttg acatcttcag
     9901 ctgaatctgg gaaaagtgga tcttgtccca tgttcatgag agctttattg gcattgtagc
     9961 gaaggaaggt tttaacctct tcagtccaac caacaccgtc ataaagactc tctgtgtagc
    10021 cttcttcatt ttcataaaga gtatagagta ggtcgtacat ccattctttg agtttttctt
    10081 gctcttcttc aggcaattca ttgaaaccaa gttggaattt gtaaccaatg taggttccgt
    10141 gaacagactc gtcacgaata atcaatttaa tgatttctgc aacgttggct agtttgttgt
    10201 taccgagata gtagagggga gtgaagaaac cagagtagaa gaggaaggtt tcgaggaaga
    10261 cgctggcaac tttcttttca agtgggctgc cgtttaggta gatttcgttg acaatctcag
    10321 ccttcttttg taggtaagga ttggtattgg tccattcgaa aatttcttca atctcagcct
    10381 tagtattcaa ggtagaaaag attgatgagt aagatttagc gtggacagat tccataaatt
    10441 ggatgttatt gaaaacagct tcctcatgtg gtgtacggat gtctgcgcga agggcttgaa
    10501 ccccagtttc agattgcata gtgtcaagaa gggttaaacc accaaaaact tttccgacca
    10561 agtctttctc tttgttagat agctttctcc agtcatccaa gtcgtttgat aagggaatac
    10621 gtgtatcgag ccaaaattgc tccgtcagtt tttcccaagt tgatttgtcg atgacatctt
    10681 cgatggcatt ccagttaatg gctttgtagt aagtttccat ttaaaatctc tttctgtgtt
    10741 tagtattgcg aactcacaat tatttctact ttaccataat tctataggag tatcgcacaa
    10801 aaagtcggaa gcccgacttt taaaatgtta cataaattat gttatgacat agtagatttg
    10861 attttatcag tgctgcttag ggaaaaataa tgtttctatg ctagaaacta aatcacacag
    10921 ctttcacatt ggttggcgcc gacttctcca ccgtcatctg taaaggtacg gacgtagtag
    10981 atagacttga ttcccttgtt aaaggcatag ttacgaagga tggacaagtc acgtgtcgtt
    11041 tgtttatttt ccctcttcca ttcgtaaagg ccttttggaa tgtcactacg catgaagagg
    11101 gtgagtgaaa gtccttgatc cacgtgttca gtcgcagcag cgtaaacatc gatgacttta
    11161 cgcatatcca tatcgtaggc agaagtgtag taaggaatgg tttctgtaga caagccagca
    11221 gcagggtagt agattttacc aattttcttc tcttggcgtt cttcgatacg ttgcgtaatc
    11281 gggtggatag aagcagaaac gtcgttgata tagctgatag aaccatttgg cgctacagca
    11341 aggcgatttt ggtggtaaag accatcttct tgaaccttgt cgcgaagttc agcccaatca
    11401 gcaacaccag ggataaagac atttttgaag agttctttaa cacggtctga tgttggaaca
    11461 aattcaccag ttacatactt gtcaaagtaa cttccgttag catagtctga tttttcaaag
    11521 ttgtggaagg taataccacg ttcacgcgca atattgtttg actctaccaa ggtccagtag
    11581 ttcataagca taaagtagat gcttgtaaat tcaacagact caggtgaacc atattcaatg
    11641 agttgttggg caaggtagct gtgcagtccc atggcaccga gaccaaaggt gtgggcttgg
    11701 ctatttccat ggtcaatcgt tggtacagct acgatatgtg aactatctgt aacgaaagta
    11761 agggcacgaa ccatagcacg gatagaacga ccaaaatcag gtgaagtcat catgttaacc
    11821 acgttggttg aacccaggtt acatgaaaca tctgttccca tttgaaggaa ttcttgagca
    11881 tcgttgatca agcttggttc ttgaacttga agaatctcag aacacaagtt actcatgata
    11941 atctttccat caacaggatt tgcacggtta gccgtatcga tgttgactac ataaggatag
    12001 ccagactctt gttgcaattt agagatttca gtttccaaat cccgcgcctt gatttttgtc
    12061 ttgcgaatat ttggatttgc gaccaattca tcgtattttt cagtaatgtc gatgtaattg
    12121 aatggcacac cgtattcttt ttctacagag taagggctga agaggtacat ttcttcattt
    12181 ttacgagcca attcgtagaa tttatcaggt actacaacac caagtgatag agtcttgaca
    12241 cgtacttttt catcagcgtt ttctttctta gttgaaagga aagcgatgat atctgggtga
    12301 aagacgttga ggtagacaac accggcacct tgacgttgcc ccaattggtt ggagtaagag
    12361 aagctgtctt caaaaagctt cataacagga acgacacctg aagcagctcc ttcatagcct
    12421 ttgataggtg caccagcttc acgaaggttg ctgagggtaa ttcccacacc accaccaata
    12481 cgtgaaagtt gaagagctga gttgatagaa cgtccgatag agttcatatc atccgtcact
    12541 tggattagga aacaagatac caactctcca cggcgagcac gtccagcatt caagaaggaa
    12601 ggagtagcag gttggtagcg ttggtggatg atttcattgg caatatcgat tgcaacagct
    12661 tcattcccat cagcgaaata aagggcgttg aagaagacac ggtcttccat attttcaaga
    12721 tagtattcac cgtcattagt ctttaaggca tattgattgt aaaatttata agctgccatg
    12781 aatgacttga attggaagtt ttggtctttg ataaattgag ctaattcttc caagaactct
    12841 ggacggtatt tcttgataaa ggctgtttcg atgtagttgt gttcaatgag gtaattgatt
    12901 ttgtctttga ttgaatcaaa aaccatagtg tttggaacta cattttcttt aaagaaagca
    12961 tccaaggctt ccttgtcttt atgaagcatg atttgtccat taacaggacg gttaatttcg
    13021 ttattaagac ggaagtaagt cacgtcttca agatgtttta atcccataaa atttccctta
    13081 tctaattaca aaagaaaggc ttctaagtta gccctaaaag cagtttcttc tggatgatgt
    13141 actaagatta tgctaattgt ttcagttttc ctggttggaa acctgaaaag acttcagttg
    13201 gtgtttggat aacaggagct gcgctaaaac cgagctcttt aacttgatcg acgtactcag
    13261 gttgctcatc aagattgatt tcacgataag agacattatt actgtccaag aaacgcttgg
    13321 tcattttaca ttggacacaa ttgtttttag aataaacggt taccattgtg taactcctct
    13381 tcaaaattta atactatctt agtatatcag aaaataaaat tttgtcaatg atttgaacta
    13441 aatatagtgg tgttcttttg ttgtttttat atataaacac aatatatagt gtttgtattg
    13501 tgaaatagca atgattatcc tataaattgt ttttaaagaa actatatcct gtgttttgtt
    13561 atctaggata gaagattttc agcaagttat ctgaaaggaa attgaaggaa aatccagaaa
    13621 atgcttgaaa aaaatcctag aagatggtat aatactaaat tgtaagggtt atcacatata
    13681 actcaaaaaa agaaagaaca aaaggagagt caaactatgg cttctaaaga tttccacgta
    13741 gtggcagaaa caggtattca cgcacgtcca gcaacattgt tggtacaaac tgctagcaaa
    13801 tttgcttcag atatcactct tgagtacaaa ggtaaatcag ttaaccttaa atcaattatg
    13861 ggtgttatga gtcttggtgt tggccaaggt gctgacgtaa ctatctcagc tgaaggtgca
    13921 gatgcagatg acgctatcgc tgcaatctca gaaacaatgg aaaaagaagg attggcataa
    13981 gggaaatgac agaaatgctt aaaggaatcg cagcatctga cggtgttgca gttgcaaaag
    14041 catatctact cgttcagccg gatttgtcat ttgagactat tacagtcgaa gatacaaacg
    14101 cagaagaagc tcgccttgat gccgctctac aggcatcaca agacgagctt tctgttattc
    14161 gcgagaaagc agtaggtacg ctcggtgaag aagcagctca agtttttgat gctcacttaa
    14221 tggttcttgc tgacccagaa atgatcagcc aaatcaagga aactatccgt gcgaagaaag
    14281 tgaatgcaga agcaggtctg aaagaagtta cagatatgtt tatcactatc tttgaaggca
    14341 tggaagacaa cccatacatg caagaacgcg cagcggatat ccgcgacgtg acaaaacgtg
    14401 tattggcaaa ccttcttggt aaaaaattgc caaacccagc ttctattaat gaagaagtga
    14461 ttgtgattgc gcatgacttg acaccatctg atacagctca attggacaaa aactttgtaa
    14521 aagcttttgt aaccaacatt ggtggacgta caagccactc agctatcatg gcacgtacac
    14581 ttgaaattgc tgctgtatta ggtacaaata acatcactga aatcgttaaa gacggtgaca
    14641 tccttgctgt taacgggatc actggagaag tgattatcaa cccaacagat gaacaagcgg
    14701 cagaatttaa agcagctggt gaagcctatg cgaaacaaaa agctgaatgg gcacttttga
    14761 aagatgctca aacagtgact gctgacggta aacacttcga gttggctgcc aatattggta
    14821 ctccaaaaga cgttgaaggt gttaacaaca acggtgcaga agctgttgga ctttaccgta
    14881 cagagttctt gtacatggat tctcaagact tcccaactga agatgagcag tatgaagcat
    14941 acaaggctgt tcttgaagga atgaacggta aacctgttgt cgttcgtaca atggatatcg
    15001 gtggagataa ggaacttcct tacttcgata tgcctcacga aatgaaccca ttccttggat
    15061 tccgtgctct tcgtatctct atctctgaga ctggagatgc tatgttccgc acacaaatcc
    15121 gtgctcttct tcgtgcgtct gttcacggtc aattgcgtat catgttccca atggttgcgc
    15181 tcttgaaaga attccgtgca gcgaaagcag tctttgatga agaaaaagca aaccttcttg
    15241 ctgaaggtgt tgcagttgcg gataacatcc aagttggtat catgatcgag attcctgcag
    15301 cggctatgct tgcagaccaa tttgctaaag aagttgactt cttctcaatt ggtacaaacg
    15361 acttgatcca atatacaatg gcagcagacc gtatgaacga acaagtttca tacctttacc
    15421 aaccatacaa cccatcaatc ctacgcttga ttaacaatgt gatcaaagca gctcacgctg
    15481 aaggtaaatg ggctggtatg tgtggtgaga tggctggtga ccaacaagct gttccacttc
    15541 ttgtcggaat gggcttggat gagttctcta tgtcagcaac atctgtactt cgtacacgca
    15601 gcttgatgaa gaaactcgac acagctaaga tggaagagta cgcaaaccgt gcccttacag
    15661 aatgctcaac aatggaagaa gttcttgaac ttcaaaaaga atacgttaat tttgattaat
    15721 cgaaaagtcc ctgcaactca gttacaggga ctttttttga tattttaaaa agaattttca
    15781 agaaaatctt tcttataaaa agtccaacct tgaaaaagta gtggtcagaa caaaaaatac
    15841 ttaaatggtt cataaaattc ttgacaagtt ggagatttag gagtaaacta ttaaccagtt
    15901 aagtaataga gaggagtttc tgcaatttag aaatgaattg caactagaaa tatcaaatag
    15961 aaagagagtt tcgatgaaaa ttaataagaa ataccttgtt ggttctgcgg cagctttgat
    16021 tttaagtgtt tgttcttacg agttgggact gtatcaagct agaacggtta aggaaaataa
    16081 tcgtgtttcc tatatagatg gaaaacaagc gacgcaaaaa acggagaatt tgactcctga
    16141 tgaggttagc aagcgtgaag gaatcaatgc tgagcaaatc gtcatcaaga taacagacca
    16201 aggctatgtc acttcacatg gcgaccacta tcattattac aatggtaagg ttccttatga
    16261 tgcgattttc agtgaagaat tactcatgaa agatccaaac tataagctaa aagatgagga
    16321 tattgttaat gaggtcaagg gtggatatgt tatcaaggta gatggaaaat actatgttta
    16381 ccttaaggat gctgcccacg cggataacgt ccgtacaaaa gaggaaatca atcgacaaaa
    16441 acaagaacat agtcaacatc gtgaaggtgg gactccaaga aacgatggtg ctgttgcctt
    16501 ggcacgttcg caaggacgct atactacaga tgatggttat atctttaatg cttctgatat
    16561 catagaggat actggtgatg cttatatcgt tcctcatgga gatcattacc attacattcc
    16621 taagaatgag ttatcagcta gcgagtt
//
LOCUS       DAVBHS010000033        16230 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVBHS010000033 DAVBHS010000000
VERSION     DAVBHS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16230
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.033"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(46..2478)
                     /locus_tag="VQ651_000775"
     CDS             complement(46..2478)
                     /locus_tag="VQ651_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2753798.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFMELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALVLGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     ALRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKVKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            complement(2480..2938)
                     /locus_tag="VQ651_000776"
     CDS             complement(2480..2938)
                     /locus_tag="VQ651_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HET2753799.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            complement(3057..3785)
                     /locus_tag="VQ651_000777"
     CDS             complement(3057..3785)
                     /locus_tag="VQ651_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2753800.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            complement(3785..4792)
                     /locus_tag="VQ651_000778"
     CDS             complement(3785..4792)
                     /locus_tag="VQ651_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2753801.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            complement(4830..5588)
                     /locus_tag="VQ651_000779"
     CDS             complement(4830..5588)
                     /locus_tag="VQ651_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2753802.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            complement(5551..5841)
                     /locus_tag="VQ651_000780"
     CDS             complement(5551..5841)
                     /locus_tag="VQ651_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HET2753803.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     regulatory      complement(5912..6014)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(6097..7443)
                     /gene="cbpD"
                     /locus_tag="VQ651_000781"
     CDS             complement(6097..7443)
                     /gene="cbpD"
                     /locus_tag="VQ651_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HET2753804.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     gene            complement(7532..7798)
                     /locus_tag="VQ651_000782"
     CDS             complement(7532..7798)
                     /locus_tag="VQ651_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753805.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            complement(7800..9152)
                     /gene="dnaB"
                     /locus_tag="VQ651_000783"
     CDS             complement(7800..9152)
                     /gene="dnaB"
                     /locus_tag="VQ651_000783"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET2753806.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            complement(9196..9648)
                     /gene="rplI"
                     /locus_tag="VQ651_000784"
     CDS             complement(9196..9648)
                     /gene="rplI"
                     /locus_tag="VQ651_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET2753807.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            complement(9645..11618)
                     /locus_tag="VQ651_000785"
     CDS             complement(9645..11618)
                     /locus_tag="VQ651_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET2753808.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDALGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNTVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            complement(11754..12302)
                     /gene="raiA"
                     /locus_tag="VQ651_000786"
     CDS             complement(11754..12302)
                     /gene="raiA"
                     /locus_tag="VQ651_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET2753809.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            complement(12382..13044)
                     /locus_tag="VQ651_000787"
     CDS             complement(12382..13044)
                     /locus_tag="VQ651_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET2753810.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQACQFWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            complement(13041..14339)
                     /locus_tag="VQ651_000788"
     CDS             complement(13041..14339)
                     /locus_tag="VQ651_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2753811.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVIEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            14395..15030
                     /locus_tag="VQ651_000789"
     CDS             14395..15030
                     /locus_tag="VQ651_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HET2753812.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVSVNLV"
     gene            15315..>16230
                     /gene="cysK"
                     /locus_tag="VQ651_000790"
     CDS             15315..>16230
                     /gene="cysK"
                     /locus_tag="VQ651_000790"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET2753813.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYE"
BASE COUNT         4788 a         3762 c         2744 g         4936 t
ORIGIN      
        1 tcactccact gtgacttatg gtcagatgct gatactttta ttcttttatg caatatcaaa
       61 ttttaactgg cctgctttga caccaatctt aagtgtgctg cctgccacta aatctccctt
      121 aagaagaagt tctgccaact tgtcctccac ttctgtttgc agggttctgc gaagtgggcg
      181 agctcccatc tctgggtcat atccttgatt tgctaacaat ttcagagctg aagcttgtaa
      241 tttcaagtca atgccttttt cagccaaact tgccactaaa ggcttgacca taatcttcac
      301 cacttcctgc atatggtcac tagataggct atggaagacc accttctcat caatacggtt
      361 gatgaattcc ggtctataag cttttttcag ttcttcaaac atgcgttttt ccatattttc
      421 ctggtcaaaa cgaatatcct tagccccaaa accaacagtc ttatcatcac gaagggcagt
      481 cgcacctagg ttcgatgtca taatgataat ggtatttgaa aaatcgacct tgcgtccctt
      541 gctatctgtc aaaacaccgt catccagaac ctgcaagaga acattaaaaa tatctgggtg
      601 ggccttctct acctcatcaa agaggagaac ggaatagggt ttattgcgaa ccttctctgt
      661 caactctcca ccttcttcgt aacccacata gcctggagga gctccattta gacggctggc
      721 tgcgaatttc tccatatact cactcatatc aaagcggata agggctgatt cgtcatcaaa
      781 gagaacttct gccaaagcct tggccaactc ggtcttaccg acacctgtgg gtcctaggaa
      841 cataaaggaa ccgatcggac gcttgtgact gcgaattcct gactgattac ggcgaatcgc
      901 acgactaatg cttgaaacag cttgatcttg accgatgaca cgtttatgga gttctgcttc
      961 aagatttaga tacttcttag catccgtttg agtcagtttt tgaactggga ttcctgacaa
     1021 gcgactcaag gtggtcaaaa tatcagactc tgtcaccaag tctttgtaga caggtacttc
     1081 ctcttctttt gcgattagct gggctgcctg tttccacttg ccatccatca gggccttatc
     1141 agctggactc aaatctgaat cgtctgcttt tacatacttt accttatttt gcactgttgc
     1201 tgctgcttcg tccaagaggt cgatagcaga gtctggcaag tgacgactgg ttaaataacg
     1261 atgagccatc ttaaccgctg tttcaaccgc ttcatctgtg atttgtacac ggtgatgttt
     1321 ctcataagtc gccttcaaac cttgtaaaat agtcatacta tctgccacac ttggttcttc
     1381 aatcgtcact ttagcgaaac gacgagaaag agccgcatct ttttcgatat gtttttgata
     1441 ttcttcctga gtggtggcac caaccgttct caaagctcca cgcgccaagg ctggtttcaa
     1501 gatattggcc gcatccagag tcgaatcaat cccgctacca gaacccatga tggtgtggag
     1561 ttcatcgata aagaggatga cttggccatc ttcttcaata tccttgatga tattattcat
     1621 gcgttcttca aagtcaccac ggaagcgtgt ccctgcaacg acattcatca aatcaagttc
     1681 taacacgcgc atcttagcca tttccgcagg cacatcacca ctggcaatac gctgagcaag
     1741 accaagcacc agagctgttt tcccgacacc agcatcccca accaagacag ggttgttctt
     1801 agtcttacgg ctcaagattt gaatcatacg tgagatttcc ttgtcccgac cgatgactgg
     1861 ttctaacttg ccagaacgcg cttgctctgt caaatcatgc gtatagtcct cgagaccacc
     1921 actaggagtc tgcggcatgc ccatcatatt ggccatagaa ttttgtttgt cagccacagt
     1981 acgatggcgt tggcgtaaag ccttgagatc ttcacgagtc cagcctgccc gttcttctaa
     2041 atttcgacga agagcagcaa tcttgacctg atctttcttg tcttcataag aaaaaccagc
     2101 cctctccaag atacgagtcg ccaaggcatt gccatcatgc aaaatcgcat agaggacgtg
     2161 ctctgtccct agtaccttag catggaccac tgacgctaca tactctgctt catcaaaaag
     2221 aacctgcaaa cgacgggaga acggcaattc cataaaggtt tcatcctggc tatagtccgt
     2281 ttcagtcagt tccaaagcca cctcttctaa acggtccatc tcatacggat aatcatttaa
     2341 agttgcccct gctacactat aactgtgatt agacatggca atcaacaagt gccacgactc
     2401 tagataacga gctccaaaat gtccagcaac catgtaggca ctttcgatac attcattcaa
     2461 tgcttttgaa tagttcatct tacttccctt ttctatctac ctcttgtatg acctgacgta
     2521 gcatgtttgc tcgaacaact ggagcttctt ctcctaaaac gcgatccaaa gctactgatt
     2581 ctagcaaatt catctcctgc ttggtcatca attcctgctc aaccaaaagc tggagaatat
     2641 cctcataaat ttcttgactg actcgctcac caatcgagta aagcagctcc cggagcattt
     2701 catgatgact agaaaactca atccgtccta tacgaatgta gcctccgcca ccacgcttac
     2761 tttcaaccaa gtagcctcta ctttccgtaa agcgtgtctt gatcacgtag ttaatctgac
     2821 taggaacaac ctgaaaggta tctgccaact gactccgttg caactccacg ataccagatt
     2881 gatctaaaat cgccttgatg taggcctcaa tatgatccga tgtattttta aatctcataa
     2941 caaacccacc tctctcttta aaccttgacc ttgtttgact attataccga aattttctca
     3001 tttttaaaag aaaaagggcg ctggtaaagg ataatcttca ccaactccct atttttctac
     3061 ttatctaagc ctaattctgc caagatttga cgtttgtagg caatcttttg gacttccttg
     3121 tcctcatctt cagaccaatc tagtttaatt tctgaaacaa tctgcccagg gcgatttttc
     3181 aagatataga tacggtcgct gagattgagg gcctcctcaa tactatgcgt gatgatcagg
     3241 gttgttagct gcaactgttt gtgaatctca agataccaag cgtggagttc catctttgtc
     3301 atctcatcca aggcgctaaa ggcctcatct aagagaaaga gcttgtgccc aaaaaggtag
     3361 gtccggagta aggctacacg ctggcgcatc ccaccgctaa gttcatgagg atacttgtct
     3421 cttacagctg tcagctggaa ggtcgcaaga attttatccg ctcgggaaat agcttctgcc
     3481 ttatccacct tttgaatcaa gaggggcaga atgatatttc caagcaccgt cttgtgctcc
     3541 aagagcagat ccttttgcaa catataactc acgcgcccct tgggattttc ttcaccatca
     3601 aggacaattc tccctgactg aacttctaaa atcccagcga ttagattaaa gagggtggtc
     3661 tttccaacac cacttgggcc tagaatggaa accacttcgc ctgaagtcac ctgtaggttg
     3721 atatcctcta aaatcctctc ctgaccatag gcataactga catgctctag tctaatttct
     3781 gtcattattt cacaaattcg ttggtgaagc ctttgtctgt caagtcttct ttaaggatac
     3841 cattttcttt atcccattta tagaaagcat tccagcgagc tgcgtcaaat tgaccccatt
     3901 tttccttgtc gcttgcgtat tcttttgaca agtatttttg agattcgatg acaaagtcac
     3961 gtttttcctt gagttcaggt gcattcttga tgagaatatc tgcagcttct tctggatgtt
     4021 ccatggcata ttggtagcct tttttgatgg cttggatgac tttgcgagct tcttctttgt
     4081 tatctttcag atagtcgttg tttgcgatga taactggtga atagtagtca aactccttga
     4141 catagtcttt caagtacatg aagttagcat ctacaccttg agatttagca aggataccat
     4201 cccaaccgta gtaaatccaa gcagtatcaa agacgccatt ggcaatcggt gtgattgagt
     4261 ttgagtcgtt atttggtact ttttcaacct tctcaaagtc tccaccttga gattctacca
     4321 aggttttcaa catagcaagt tcagttgggt cattccatgt cccatatttc ttaccaacca
     4381 agtcttttgg actgcttaca ttatcagatt tacgagagat gattcctgat gtattgtgtt
     4441 caacaatagc tgcaacggca gtgattcctg ctcctttttc caatttctta gccatgtagt
     4501 cttggaaata cactgcaaat ggtgcctttc cgttgataac caagtcagaa gaactttctt
     4561 ctggtggcaa tttcaaatca acatccactc cagcttcttt gaaataacct ttttccttgg
     4621 caacataaag ccctgtgtgg ttggtatttg gtgtccagtc taggataaag tcaaccttct
     4681 taagttctgc ctctttgttg tctttagaag cagttccttg accacaggcc acaagcacaa
     4741 cagctacaag agctgttaca agcgttaaaa acactttcca tgttttcttc attttgattt
     4801 cctcttttct ttttcaaaaa cgctctattt taagtacgtt tccatttaat cacatatttt
     4861 tcactgatat cgaccagctt catacccaaa agactgataa tcgacaccag aataataatg
     4921 gcaaacatgg tatcatactg aaacagtttt ttagactgaa tcatataaac accaagacct
     4981 tcaaaacctc ccaaccactc agataccaca gttgtgataa aggcgtagga gacactgacc
     5041 ctcagacctg cataaaagta aggcaggcta accgggattt taaaatgcca caggatttgc
     5101 caagggtttg ctcgcatcag actaaacaaa gtcagcatat ccttgtcaca atgcctaaaa
     5161 ccgtccaaaa tactaacgat gatgggaaag gttgttgtta agataatcaa gacaatcttg
     5221 ggcaaaatcc cataacccag ccacaagacc aggataggag ctatggcaat ggtcggaatg
     5281 gtctgaatga ccaccatcat agggtaaatc aggtcattga gccaagtcaa actatccatg
     5341 agcacagcca taagacaggc aatcaaaact cccaaaatca gccccagtaa agccactctc
     5401 aaggtcgccc agctatggtg ccagagaaat tctctgtcac gaacaaaggg ctggagaatt
     5461 tcaagaggtg tcggcaggat aaacttgggg agaagtttaa gaaaacctgc taactgccag
     5521 attgacaata ctccgagaaa gcccaataga ctaatgtgtc gtctcagtat acttttcaag
     5581 tttctcatca atacttaaaa tctctcctac atttattttg acattggcaa agacattgtc
     5641 tgcctcctgc cctgccactt ccagcgcttc ttttagaatg cgcataagct catcaaactc
     5701 cccttccaag accgtttcaa atggtgtcac taccatcgtc acttcttgag tttgcagata
     5761 agcaatgacc tgatcaataa cagctatccg atcaatcccc tgtgctaggg gtaaaacttg
     5821 caaggcaatg cttgctttca taagaacctc ctcttctcgt tttcctttta aaaaaaagaa
     5881 aaacctttac catacatgga aaaggtgcaa aattagacta agtatcgttc cctccgctgg
     5941 cattacccag atcaggtgcg gtcgaagttt aacacttcct ctcagactgt acacagactc
     6001 ccatatatta tatgtatata tgatagcgca aaataagaaa aatgtcaagg aaactgctta
     6061 cagaaaagag caggaaattt ctcctactcc aattttctat actcgttctc catcactgtt
     6121 tactctgtag ccatccaccg tcgtattaac agctaaggcg cctgaagagt aagaataata
     6181 ccacttacca gagacctggt accagcctgt tttcattgcc cctgaactac ccagataata
     6241 ccacgagcca ttctccttta accatcctgt ctgcatttca ccagataatt tcaaataata
     6301 ccagctagaa ccgtctttca gccatcctgt tgatttagaa ccatttgatt tgaaatgata
     6361 ccaactacca tttattttct tccaaccgac agttgaacta ttattggatc catagctaga
     6421 gatatttcct gattgattct gataattttg tgaagaagtc actccctcta gctgtatata
     6481 gcgacgactt ccattatagg cagtataact caaccacttg tagccgtctt tttcaagtat
     6541 ctgatcataa tgaaccttct ccccaggata atagtaatca atcacagttc cgctaactag
     6601 gggttcagtt ttgatagcag acttggtctt aaaataatga gttccacctg ttgaagaaag
     6661 aacagaatta cctagaggat ttttattcac agcctccaat tgaacataac gatagcttcc
     6721 attataggca gtataactca accacttgta gccgtctttt tcgagaattt gatcataatg
     6781 aaccttctct ccaggataat agtaatcaat cacagttgca ctaactaggg gttcagtttt
     6841 aatagcagac ttggtcttaa aataatgagt tccgcctgtt gaagttgagg attgactatt
     6901 cccaacactg ccaccatcca aatctttaaa atgaataaat cctgtcatcg tgtttgcttt
     6961 tataactcgt ttattatagg attctgtata accatagtta tattcctcaa tctcaatctg
     7021 atctcccatt acatttgaca cccaggcaac atgaccataa gttcctgcag tagaccaagt
     7081 aatggaacca atcgtcggtg tattatctac acgataacct tcccgacgag cacgatgtcc
     7141 ccattcattc gcatttccat aagctgccgg aatttcaaaa ccattgacat tactcaaacg
     7201 aaaggctaca aaagaagtac actgacgaga atacatgcgc cactgatcaa tctcctggct
     7261 cccattttta taataagcag gataatcatc cccacgcgca atcgatccat ttcctctgga
     7321 ataggcatag acactatcac cagctgctag catcaatcct actactaaaa aaggaacaag
     7381 ctttttaact gacatcttca agtaataact tgttcctctt gctataaacg gtaaaatttt
     7441 cattcttcct ccttgaaaaa taatataaag cgatgattgc cttcacctaa attattcgga
     7501 gaaaaaagaa aaagtgagaa aatttctcac tttagtcaag ataatgaatc aaattctttt
     7561 ctgtaagaat atctgagtaa gtaaaggatt caacgtaaac attaacttgt ttatctcctt
     7621 ccacatcccc aaactccaca ataaatagag atggataaac ttcaattagc ttacccaatc
     7681 tatttttttg gcgcttacga ccattctcca aagtcatttc tacgacttgt ccctcatgtg
     7741 ccttgatttc ttctttgatt tttttcatct tggctacatc tgtaaatgca tctgacatct
     7801 tatgcctccc tctttgagat acttgaaaat ttattgtatt ctttttggaa aatcaattcc
     7861 actgttccac gagctccact acggtttttc tcgataataa cttccacctt attatttggg
     7921 ataccctcct cttcttcacc accacgttca tagtagtcat cgcgatagag aaaagctacg
     7981 atatcagcgt cctgctcaat agacccagat tcacgaatat cagacaagac cggtctcttg
     8041 tcctgacgtt gttctacacc acgagaaagc tgactcagag cgattactgg aaccttcaat
     8101 tccttggcta gtattttcaa ctgacgagaa atttcagaaa cttcttgttg acgattttct
     8161 cgaccagttc ccgtgataag ttgcaaatag tctatcaaaa tcaaaccaag atttccagtt
     8221 tcttgagcca atttacgaga acgagaacga atctctgtaa tccgaatacc tggcgtatca
     8281 tcgatataga tactggcgtt agctagatta ccctgagcaa tagtatattt ttgccactcc
     8341 tcatctgtca attgccctgt acggatagaa tgtgactcca ctaagccttc tgcagctaac
     8401 atacgatcta ccaagctttc cgcacccatt tcgagtgaaa aaatagcaac cgttttgtcc
     8461 aacttagtcc caatgttctg agcgatattc aaggcaaatg ctgtcttacc aactgctgga
     8521 cgagctgcta agataatcaa ctcctcctca tgaagtcctg ttgtcatatg atccaaatca
     8581 cgataacctg tcgcaatacc tgtaatatcg gtcgtttgtt gcgagcgagc ttccagattt
     8641 ccaaagttga gattcaacac atctcgaatg ttcttaaacc cgcttcgatt tgcattttca
     8701 ctgacatcaa tcagcccttt ttctgcctga gcaataattt catcagctgg ttgtgacgct
     8761 tcgtaagctt ggttgacaga ctctgtcaac ttggcaatta aacgacgtag cattgctttt
     8821 tctgcaacaa tcttagcata atactccgca ttagcagaag ttggcacaga attaacaatc
     8881 tcaaccaagt aagacaagcc accaatattc tgtaaatcac cttgattatc aaggatagta
     8941 cgaaccgttg ttgcatctat ggcatcacca cgatcggata aatcgaccat ggcttggaaa
     9001 atcaaacgat gggcatactt aaaaaagtcc cgagactcaa tgtattctcg cacaaaaaca
     9061 agtttactct catcaataaa gatagcccct aaaacggatt gctcagctaa gatatcttga
     9121 ggttgtactc gtaactcttc tacttctgcc atcagacttc ccttcctttt acaatcttgt
     9181 caagaaggtg taaacttatc cttctttcac acgaagattg attacacttg tgatatcttg
     9241 atagattttc actggcacat caatcaaacc aaccgctcga atcggagctt gtacttgaat
     9301 atgacgttta tcaatcttaa ttccaaattg tttttgcaat tcttctgcaa tcttcttatt
     9361 ggtaatagag ccaaaggtac gaccatctgg accaactttt tcaacaaatt ctacaacagt
     9421 ttcttctgct tcaagttgtg ctttaattgc ttttccttct gcaatcatct cagcgtgagc
     9481 tttttcttcc gatttttgtt taccacgaag ttcacctaca gcttgagcag tcgcttcttt
     9541 ggctagattc tttttgataa gaaagttttg cgcataccct gttggtactt ccttaatttc
     9601 gcctttttta ccttttcctt taacatctgc taaaaagatt actttcattc ttctttctcc
     9661 ttttccttta tttcatttaa tacaatttct gtcagttttt cacctgcttc tgacaaggtt
     9721 acatctttaa tttgagctgc tgccaaatta aagtggcctc caccgcctaa ctcttccata
     9781 atccgttgta cattcagttt actacgactt cgagctgaga tagagataaa tccttgtgta
     9841 ttcttcgcaa gaacaaaact cgcttcaata cctgacatgg ctaacatggc atctgctgcc
     9901 ttactaataa caactgtatc atagcatttc gtgtccttag cctctgctat tagtacatct
     9961 gaacctaatt tacgcccctg taaaataagt tcattgacct cacgatattc ttcaaaatct
    10021 gtcgcagcga tttcctggat agcaatacta tcacttccgc gcgttctgag atagctagca
    10081 acatcaaatg tccgactagt tactcgcgag gtgaaatttt tagtatccaa catcatacca
    10141 gccatcaaga cacttgcttg catacgactc aaacgatttt tcttagaatt ctggaactga
    10201 atcaattccg ttaccaactc actggcacta cttgcaccac tttcgatata agtaatgacc
    10261 gtattatctg gaaaatcctg atcccttcta tggtggtcaa taacaatggt ttgggtaaat
    10321 aaatcataaa attcttttga taatgttaag gctgtctttg aatggtctac aagaatcaac
    10381 aaagaacgat tggtcaccat ccccaatgca tccttaacag acaacaactt cgtaactcct
    10441 tctttttcta tgaatgaaac agctcgttca atatctggag acatttgttc ttcatcataa
    10501 agagcatagc tattttcaat cacattgctg gcgaacaact gcatacctac agcagagccc
    10561 aaagcatcca tgtctaaatt tttgtgaccg actacaaaaa cctgatctac actccgaatc
    10621 ttatctgaaa tagctgtcat catagcgcgc gtacgagtcc gtgtacgctt gattgaagca
    10681 gcagacccac caccaaaata aactggattt ttcgtttcgt cgttttcctt aacaaccacc
    10741 tggtcgccac cacgtacttc agccaagttc aaattgagca aagcaacttt ccctatctca
    10801 tcatgatttc catcgccata agaaaatccc atacttaagg tcaagggcaa ctgtctctgt
    10861 ttcgactctt ctctgaaagc atcaataaca gaaaatttat cattcatcaa gccctcaagc
    10921 accgtgtagt cagtaaatag ataaaatcga tccatactta cccgacgaga aaacatcatg
    10981 tatttttctg aaaactctga tataaaatta gctacaaaac tattgatttg actaatatct
    11041 gactcagaag ttgcatcctc caaatcatca taattatcca cagagacaat cccaatcact
    11101 ggtcgacttg ttaccaattc atctgttatg gcttgttccc tggatacatc tacaaaatac
    11161 aaaacaccgg aagaagcatc catatgaaca gcataacgct tctcaccaag cttggcataa
    11221 gtagacggat ttcctactga agccttgata atcgtttgaa cagcttctaa atcaaaatca
    11281 ccatcttcct tggtcaaaat caattcagca tagggattaa accactcaac ctctccagaa
    11341 gataaattca atttcataac acctacaggc atctgttcca atagagctgt caaactttct
    11401 tccgcttggt ggtttacata ctgtatctgt tctacatcac tccttgtata atgcactctc
    11461 agtttcttaa ataaaaaaac atagcctcct acaaaaagaa acaaaattaa aaccgtcaac
    11521 agattattat taacaaaaat aatgaaagtg gataagactc caaacgcaat caatcctact
    11581 agaataggaa aaattggact tacataaaat tttttcattc aaaacctctt ggcacccatt
    11641 ataccataat acccctcaaa aagcgacttt ttaaaagtgt aatcagtaat tctatcaatt
    11701 ataagaaaaa ggtagtttac aattcagtaa acctaccttt acacatattg aaattaagat
    11761 tctttaacct ctaacaaacc aatttcgcca tcctcacgac gataaatcac attggttgtc
    11821 tgatcttcaa catccacata gataaagaaa tcatgcccca ataaatccat ttgtagaatt
    11881 gcttcttcca aatccattgg ttttaaatca atttgttttg aacgaacaac tttagactgg
    11941 acaatatttg aatcttccac caaagcatct gtaaataatt gaccagttgc taccttattt
    12001 ttatttttac gctcgatttt tgttttattt ttacgaatct gacgttcaat tttatcagtt
    12061 acaaggtcaa ttgaaccata catatcttga gatacatctt ctgcgcggag agtaatagat
    12121 ccaagcggaa tcgttacttc cactttagcc gttttttcac gataaacttt taagttaatt
    12181 cgggcatcca actcttgttc tggttggaag tacttttcga tcttttcgag tttagaaact
    12241 acataatcac gaattgcttc tgttacttct aggttttcac cacggatact atatttaatc
    12301 atatgagtac cttctttcta aacatttttg tttttatgat tttattataa cgctttcatt
    12361 ctatttttgc aaattttttc ctcatcttac aagggaaaat gtttttacat ccttagcacc
    12421 agcttcttcc aacagtttct taacacgatt tatagttgct cctgtagtat agatatcatc
    12481 tataagtagg atttttttag gaatagtgac tccactttta ataaagaaag gaagttctgt
    12541 ccccaagcgc tctgaacgat ttttagaaga actggctctc tcttctcttt tctctaataa
    12601 atctagatac tcaaagcctg ctgcctctac caagccctca acctgattaa atcctctgtt
    12661 agcatatcta tcaggactta ggggaattac aacaaattga tactctttgt actttttcaa
    12721 ctcctcactt aaaaatgaag cgaaaacttt tcttaacagg aagtctccat caaacttata
    12781 ccgactgaaa aaatccttca tagcttgatt gtaagtaaaa atcgctctat gactgacttc
    12841 aactccttct ttacaccaaa attgacaagc ttgacacttt gttgacaact ctgttttcat
    12901 acaatttgga cagttctctt ccccaattct ttcaaaagta gaatcacagt ctgaacaaag
    12961 acaagagtca tcattcctca gaagtaagag actactaaaa gttaaaacag tcttcatagt
    13021 ctgcccacat aacaagcact tcatagacca gcctccttat tcatcatctg aatttcctta
    13081 atcgccttct tgattgaagc atttaaccca tcatggaaga aaagcaaatc tcctgtcggt
    13141 ctatccatgc ttcgtccaac tcgtccacca atctgaatca aactagactt ggtaaacaaa
    13201 cgatgattgg cctctattac gaaaacatcc acacaaggga aggtaactcc acgctccaag
    13261 attgtcgtac tgataagtat tgtcagttct ccatctcgaa aagcttgtac ttgctctaat
    13321 cgatcctctg ttacagaaga tacaaagcca attttctcat ttggaaattg ctcctgtaag
    13381 atttcttcta actgctcccc tttcttaatt tctgaagcaa aaatgagtaa cggataagct
    13441 gtctttctct gcttctcaat ataggacttt aactttggtg acaaacgatt cttgtctaag
    13501 tagcgattaa aatccgataa ccaaattggt tttggaataa tcaacggatt tccatgaaac
    13561 cgtctcggta aactcagtct ttttagttct cctaaacgga cctttttatc taactcattg
    13621 gtcgaagtcg ctgttaaaaa gattctcaat ccattctcct ttacactatt cttgacagcg
    13681 tggtaaagcg tgggattatc aacataagga aaagcatcta cttcatccac tatcagcaaa
    13741 tcaaaagctt gataaaactt caataactga tgggttgttg caacaactag tggtgttcga
    13801 aaataaggtt ccgattctcc atgtagcaaa gctataccgc aagaaaaatc ctgttgcagg
    13861 cgcttgtaca gctccaaaca aacatctatg cgaggactag ccaaacacac tgcaccaccc
    13921 gcattgatca ctttagccac tacttgataa atcatttctg tctttccagc tcctgttacc
    13981 gcatgaacta aggttggctt ttgcttgtct actgcttgaa gcagtccctc tgacaccttc
    14041 tcttgaaaag gagttaattg gccgcgccat ttgagaacat cttgcttcgg aaaatcctcc
    14101 tgcggaaaat agtataaagt ttgatcactt ctgactcgct tcatcagcaa gcactctcga
    14161 caatagtaag caccgatggg caaataccat tcttctagaa tagtactatt acaacgttga
    14221 cagaaaagtt tccccttctc ctttctcatt gctggaagtt tctccgccaa ctgacgttct
    14281 tcttctgtta attcattctc agtaaataaa cgaccgagat aatctaaatt tactttcata
    14341 cttctttatt cgtaaaaact agcactttag attatttttt agtacaatta aatcatggaa
    14401 tttaggacaa ttaaagagga cggccaagtc caagaagaaa tcaaaaaatc tcgctttatc
    14461 tgccatgcca agcgtgttta tagcgaagaa gaggctcgtg acttcattac tgccatcaaa
    14521 aaagaacact acaaagcgac acataactgc tctgccttta ttattggaga acgtagtgaa
    14581 attaaacgta caagtgatga tggtgagcct agtggtactg ctggtgttcc catgcttggg
    14641 gtactagaaa atcacaatct caccaatgtc tgtgtggtcg tgacacgcta ctttggtggt
    14701 attaaactag gcgctggagg actaattcgt gcttacgccg gcagtgtcgc cttagctgtc
    14761 aaagaaattg gtattattga aataaaagaa caggctggca ttgctattca aatgtcttat
    14821 gctcagtacc aagagtacag taacttcctt aaagaacatg atctcatgga gctggataca
    14881 aactttacag atcaagtcga tacgatgatt tatgttgata aagaagaaaa agaaactatt
    14941 aaagctgcac ttgtggagtt ttttaatgga aaagtcactt taactgacca aggtttacga
    15001 gaggttgaag tttctgtaaa cttagtgtaa acaatgaata atacagcgtt tcgttgacat
    15061 tctcacaact actttagcga gcaaaataaa aagatgcgta ccaaaatata ctagaaaatg
    15121 aagcaattca aacgaaacct gatatcgttt tccttcacac ctatttacta gaattaactg
    15181 aacgcaatca cttgaaaatt aatgactttg atctatgata tatagaaatc ctatcgcttc
    15241 gataggattt ctatatttta caaatggtat ggatagcgtt atactaaaga tatcttatac
    15301 aaagaggtat tcatatgtct atttataaca acattactga attaatcggt caaacaccga
    15361 ttgttaaact taacaacatc gtgccagaag gtgctgcaga cgtctatata aagcttgaag
    15421 catttaatcc tggttcatct gtaaaagacc gtattgccct tagcatgatt gaaaaagctg
    15481 aacaagatgg tattctgaaa cttggttcta ctattgttga agcaacaagt ggaaacaccg
    15541 gtattggact ttcatgggta ggtgctgcta aagggtataa agtcgtcatc gttatgcctg
    15601 aaactatgag tgtagaacga cgtaaaatta tccaagctta tggtgctgaa ctcgtcctaa
    15661 ctcctggtag cgagggaatg aaaggtgcta ttgctaaggc tcaagaaatc gctgctgaac
    15721 gtgatggttt ccttcctctt caatttgaca atccagctaa tccagaagta cacgaaagaa
    15781 caacaggagc tgagatacta gctgctttcg gtaaagatgg attagatgcc tttgttgctg
    15841 gagtaggtac tggtggaacg atttctggtg tttctcatgc actcaaatca gaaaattcta
    15901 acattcaagt ttttgcagta gaagcagatg aatctgctat tctatctggt gaaaaacctg
    15961 gtcctcacaa aattcaaggt atctcagctg gatttattcc tgatacactt gatactaaag
    16021 cctatgatgg tatcgttcgt gtaacatcag atgacgctct tgcactcgga cgtgaaattg
    16081 gtggaaaaga aggcttcctt gtagggattt cctcagctgc agctatctac ggagccatcg
    16141 aggttgccaa aaaattaggt acaggtaaaa aagtccttgc cctagcacca gataacggtg
    16201 aacgttatct ctctacagca ctttatgaat
//
LOCUS       DAVBHS010000034        16181 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVBHS010000034 DAVBHS010000000
VERSION     DAVBHS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16181
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.034"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            66..287
                     /locus_tag="VQ651_000791"
     CDS             66..287
                     /locus_tag="VQ651_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2753814.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            804..1043
                     /locus_tag="VQ651_000792"
                     /pseudo
     CDS             804..1043
                     /locus_tag="VQ651_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1187..2416
                     /locus_tag="VQ651_000793"
     CDS             1187..2416
                     /locus_tag="VQ651_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET2753815.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2436..5132
                     /gene="ppc"
                     /locus_tag="VQ651_000794"
     CDS             2436..5132
                     /gene="ppc"
                     /locus_tag="VQ651_000794"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET2753816.1"
                     /translation="MSLQKLENSSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATNLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYEIILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5215..5432
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5545..6549
                     /locus_tag="VQ651_000795"
     CDS             5545..6549
                     /locus_tag="VQ651_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2753817.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     IELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTIKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            6618..7484
                     /locus_tag="VQ651_000796"
     CDS             6618..7484
                     /locus_tag="VQ651_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2753818.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            7481..8239
                     /locus_tag="VQ651_000797"
     CDS             7481..8239
                     /locus_tag="VQ651_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2753819.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            8329..10089
                     /gene="dnaG"
                     /locus_tag="VQ651_000798"
     CDS             8329..10089
                     /gene="dnaG"
                     /locus_tag="VQ651_000798"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET2753820.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10092..11201
                     /gene="rpoD"
                     /locus_tag="VQ651_000799"
     CDS             10092..11201
                     /gene="rpoD"
                     /locus_tag="VQ651_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET2753821.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11215..11544
                     /locus_tag="VQ651_000800"
     CDS             11215..11544
                     /locus_tag="VQ651_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET2753822.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            11659..12702
                     /gene="cpoA"
                     /locus_tag="VQ651_000801"
     CDS             11659..12702
                     /gene="cpoA"
                     /locus_tag="VQ651_000801"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HET2753823.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            12718..14043
                     /locus_tag="VQ651_000802"
     CDS             12718..14043
                     /locus_tag="VQ651_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2753824.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGILIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGALYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFRTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14074..14250
                     /locus_tag="VQ651_000803"
     CDS             14074..14250
                     /locus_tag="VQ651_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET2753825.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            14309..14443
                     /locus_tag="VQ651_000804"
     CDS             14309..14443
                     /locus_tag="VQ651_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HET2753826.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            14507..15817
                     /gene="obgE"
                     /locus_tag="VQ651_000805"
     CDS             14507..15817
                     /gene="obgE"
                     /locus_tag="VQ651_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET2753827.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            15827..15985
                     /locus_tag="VQ651_000806"
     CDS             15827..15985
                     /locus_tag="VQ651_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753828.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
BASE COUNT         4960 a         2827 c         3608 g         4786 t
ORIGIN      
        1 ccagttagga cactttgaat gatttcttag ttttatatat tatacttttc tcaggagaaa
       61 gctagatgta cagacgtttg agagatttga gggaagatca cgatttgacc caaaaacaaa
      121 tagctaaaat actttcgttt acaaactcag cttatgctaa aattgaacgg ggtgagcata
      181 cgttaacagc agatgtattg gtaactctct caaactttta cgatgtcagt acagactacc
      241 tattggaatt gacagatttt cctgataaaa ttcgctttag aaaataatct cctcaatttc
      301 atagagtgtg aaaatgagga gattttttat ttgttctttg aaaattaaat agtccaaaat
      361 ggtactttcc tcatttgtgg agcaaatttg aatggctcgc catgataaga gcgattttaa
      421 agccatcaat aaaatagagc gatactttat atgccatgat acaaatgata tacaatgata
      481 cttctgaccg ttcaggctgc catcgtaaaa gagcagcaag tgaaattctt atgatgactt
      541 catcagtcat gccacgttga atgtgtgagt ttgttagaga aacgcaatta atcctcaaaa
      601 ggttccccga accttttgag ttccacagac gcatcacgtg gagtgtgtaa gcttgttgct
      661 aaaagcgtaa aaaccttgga acgaaaggaa taatagactt cctgcgaaac aaaaatacaa
      721 tacaataaaa ctatggatga tgaagcaagt aaacaattaa gcgatagccg tttcaagagc
      781 cttgtaggtg ttcagcgcac gagatgttag ctgtgttaaa aacagcttat caacgcaacg
      841 taaacgcgca aaaggtggac gaaaaaccaa attaagccta gacgatctcc tcatggtaac
      901 tattcaatac atgcgagaat agcgcactta tgaacaaatt gcggctgatt ttggcattca
      961 cgaaagcaac ataattagtc ggaggtcaat ggattgaagc aactcttatt caaaatggtt
     1021 ttacgatttc aaattctgcc ttaattctgt aaaaacagta aaattcgaag gattgtaagg
     1081 taagagtttt tttctttctg aaaaaatggt ataatagcaa tcaaaactag aaaataaaac
     1141 ggaatttgga acagatttgt ctgtatccta gtagagtggt gatactatga agattagtaa
     1201 gaggcactta ttaaattatt ccatcttgat tccctacttg cttttatcta ttttgggctt
     1261 gattgtggtc tattcgacca ccagtgctat tttaattgaa gaaggcaaga gcgccttgca
     1321 gttggttcga aaccaaggaa tcttttggat tgttagtttg atactgattg ccttaattta
     1381 taaattgaga ctagattttt tgagaaatga gcgactaatc attttagtta tattaataga
     1441 aatgctttta ttgttcttgg ctcgttttat tggtatttca gtaaacgggg catacggttg
     1501 gatttcggtt gcaggagtaa ctattcagcc agctgagtac ttaaaaatca ttattatttg
     1561 gtatttagct caccgattct ccaaacagca agaagaaata gctacttatg attttcaagt
     1621 tttgactcaa aatcaatggc ttccccgtgc ttttaatgat tggcgattcg ttctcctagt
     1681 tctgattgga agtttgggaa ttttccctga tttaggaaat gcgactattt tagtcttggt
     1741 ttccttgatt atgtatacag ttagtggaat cgcttatcgc tggttttcaa ccattctggc
     1801 gctcgtatct gccgcttctg tctttgtctt gaccactatc agcctaatcg gtgttgagac
     1861 cttttcaaaa attccagtat ttggctatgt agccaaacgc tttagtgcct tttttaatcc
     1921 ttttgccgat cgtgctgatg caggtcacca gttagctaat tcttattttg ccatggtcaa
     1981 tggtggttgg tttggtctag gtcttggaaa ctcgattgaa aaacgaggtt atttgccaga
     2041 agctcataca gactttgtct tttctatcgt gattgaagaa tttggctttg ttggtgccag
     2101 tcttatttta gctctcttgt ttttcatgat tttgcggatt atcttggtcg gtattcgagc
     2161 ggagaatcct ttcaatgcca tggttgcact cggtgtcgga gggatgatgt tggttcaggt
     2221 atttgtcaat atcggaggga tttcgggctt gattccatct acaggagtaa ccttcccctt
     2281 cttatcccag ggtggaaata gtcttctagt cttatcagtg gcagtagcct ttgtcttaaa
     2341 tattgatgcc agtgaaaaac gcgctaagtt gtaccgagaa ttggaaaatc aaccaatgaa
     2401 ccttctgttg aagtaggata aagaaaggat agtttatgtc tcttcaaaaa ttagaaaatt
     2461 ctagtaataa aagtgttgtg caagaagaag tcttgattct aacagaatta ctggaagata
     2521 ttactaaaaa tatgcttgcc ccagagacct ttgaaaaaat aatacagttg aaagaattat
     2581 caacgcagga agattatcaa ggtctaaacc gtctagtgac tagcttatca aatgatgaaa
     2641 tggtctatat ttcacgctat ttctctatct tgcctctttt gattaatatt tcagaggatg
     2701 tggatttagc ttatgaaatc aatcatcaaa ataatattga tcaggactat ttaggtaaat
     2761 tatctacaac gattaaattg gtagcagaaa aggaaaatgc cgttgagatc ctagaacact
     2821 tgaatgttgt tccggttttg acagcccatc caacacaagt gcaacgcaaa agtatgttgg
     2881 atttaacaaa tcatattcat agtcttttgc gtaaataccg tgatgttaag ttggggttga
     2941 tcaataaaga taaatggtac aatgatttgc gtcgttacat cgaaattatc atgcagacag
     3001 acatgattcg tgagaaaaaa ttaaaagtga ctaacgaaat cacgaatgct atggaatatt
     3061 ataacagctc ctttttgaaa gctgtacctc atttgacgac ggagtataag cgcttagcgc
     3121 aagcgcatgg tctgaattta aaacaggcta aaccaatcac catgggtatg tggataggtg
     3181 gtgaccgtga tggaaatcca tttgttacag caaagacctt gaagcagtct gcactcactc
     3241 aatgtgaagt catcatgaac tactatgata aaaagattta ccaactttat cgtgaatttt
     3301 ctctttcaac tagcattgtc aacgtcagca agcaagtcag agaaatggct cgtcaatcca
     3361 aggataactc gatttaccgc gaaaaagagc tttaccgtcg tgccttgttt gatattcaat
     3421 caaaaattca ggcaactaaa acctatctga ttgaggatga agaagttggg actcgttatg
     3481 aaaccgccaa tgatttctac aaggatttga ttgccattcg agattctcta ctagagaata
     3541 agggcgagtc cttgatttca ggtgattttg tggaattatt gcaggcagta gagatatttg
     3601 gtttttacct agcatcaatt gatatgcgac aagactctag cgtctatgaa gcctgtgtgg
     3661 cagaactctt gaaatcagca ggaattcatt ctcgttatag cgagttgagc gaagaagaaa
     3721 agtgtgacct tcttttgaaa gaattagaag aagatccccg aattctttct gcgactcacg
     3781 cagaaaaatc agaattatta gcaaaagaac tagctatttt taagacggct cgtgttttga
     3841 aagataagtt gggagatgat gtcatccgtc agaccatcat ttcacatgca accaaccttt
     3901 ctgatatgct agaattagct attctgttaa aagaagtagg actggtggat acggaaaggg
     3961 cgcgtgttca gattgttccc ctttttgaaa caattgaaga cttggatcat tcagaggaaa
     4021 caatgagaaa atatctttct cttagccttg ccaaaaaatg gattgactca cgaaataact
     4081 accaagaaat catgcttggc tactctgaca gtaataaaga tggcggttac ttgtcatcat
     4141 gttggaccct ctacaaggct caacaacaat tgactgctat tggagatgaa tttggcgtta
     4201 aggttacctt cttccatggt cgtggtggta ctgtcggtcg tggtggtggg ccaacctatg
     4261 aagccattac atctcaaccg ctcaagtcta tcaaggatcg tatccgtttg acggagcagg
     4321 gtgaagtaat tgggaataaa tacggtaaca aagacgccgc ttactataac cttgaaatgc
     4381 tagtatcggc agctattaac cgtatgatta ctcagaagaa gagcgatacc aataccccaa
     4441 atcgttatga agccattatg gatcaagtag tggaccgtag ttacgatatc taccgtgatt
     4501 tggtctttgg taatgagcat ttctatgatt atttcttcga gtcaagtcca atcaaggcta
     4561 tttcaagttt taatattggt tctcgtccag ccgctcgtaa gactattact gaaatcggtg
     4621 gtttgcgtgc catcccttgg gtattctcat ggtcacagag tcgtgttatg ttccctggat
     4681 ggtacggggt tggttcaagc ttcaaggaat ttatcaataa aaatccagag aatattgcta
     4741 tcttacgaga tatgtaccaa aattggcctt tcttccaatc gcttctttca aatgttgata
     4801 tggttttgtc aaaatcaaat atgaatattg cttttgaata tgctaaactt tgtgaagacg
     4861 agcaagttaa ggccatctat gagattattt taaatgaatg gcaagttact aagaacgtta
     4921 tcttggctat tgaaggacat gacgaactct tagctgacaa tccatatcta aaagctagtc
     4981 tggattaccg tatgccttac tttaatattc tcaactatat tcagttagag ttgattaaac
     5041 gccaacgtcg tggagaattg tccagtgatc aagaacgatt gattcatatc accatcaacg
     5101 gaattgcgac aggattgcgt aattcaggtt gataattttc aagagtgaat gctaaaagtg
     5161 aatatcaaaa aaattctaat agactattga caagtagttt aaaaatgata taatttaacc
     5221 attcagaaaa gtaatcatac aaacttttta gagagtctgt ggtagctgaa aacagataag
     5281 tggcgatgat gaaaattggg ctgaatgcta tttagaattt gaaattataa aaattcggta
     5341 agcacacctt acagtgcatc tcgttattgc gagactgagc gatagggaaa ttccctataa
     5401 ttgaggtggt accgcgcatc gacgtcctca cacaagtttt ttgtgtgagg atttttttga
     5461 tggaggttag tatggaaaga aaacgatggc gtcgcttgtt tagataagtg aaatatgtta
     5521 aaggaaataa aaaggagaaa cagaatgaaa aataaacgtt taattggaat tattgctgca
     5581 ttagcagtct tagtagcagg aagcttgatt tattcttcaa tgaataaatc agaagctcag
     5641 aataataagg atgagaagaa aataaccaag attggtgtgc ttcaatttgt gagccatcca
     5701 tcccttgatt tgatttataa agggatccaa gatggacttg cagaagaagg atataaagat
     5761 gatcaagtta aaattgattt tatgaactca gaaggtgatc aaagtaaggt tgcgacaatg
     5821 agtaaacaat tggttgcaaa tgggaatgac cttgtggttg gtattgcaac accagcagcc
     5881 caagggttgg ctagtgcaac aaaagaccta ccagttatca tggccgctat tacagaccca
     5941 attggtgcta acttggttaa agatttgaaa aaaccaggtg gtaacgttac aggggtatct
     6001 gaccacaatc cagctcaaca acaaattgaa ctcatcaagg ctctgacacc gaatgtgaaa
     6061 acaatcggag ctctttactc aagtagcgaa gacaattcaa aaacacaggt tgaagaattt
     6121 aaggcttatg ctgaaaaagc aggtctgaca gtggaaacat ttgcagttcc ttcaacaaat
     6181 gaaattgcct caactgtcac tgttatgact atcaaggtag atgctatttg ggttccaatt
     6241 gataacacca ttgcatcagg atttccaacg gttgtctcta gcaatcaaag ttctaagaaa
     6301 ccaatttatc ccagtgcgac agctatggta gaagtaggtg gtttggcatc agttgtaatt
     6361 gaccaacatg accttggtgt ggcaacaggt aaaatgattg tgcaagtctt gaaaggtgca
     6421 aaaccagccg ataccccagt caatgtcttt tcaactggta agtcagtcat caataaaaaa
     6481 atagcacaag aactaggtat tactattcct gagtctgttc tcaaagaagc aggacaagtc
     6541 atcgaataaa taaggtaggg aggaggagac tcttcccatt ttttaaaaag atgaatatag
     6601 aaaggattaa ataaaatatg attgtttcca ttatttctca aggatttgtc tgggctattc
     6661 taggtctggg aatctttatg acatttagga ttttaaactt tccagatatg acgacagaag
     6721 gttccttccc tcttggggga gctgttgctg tcactttgat aaccaaaggc gtgaacccat
     6781 ttttagcgac acttgttgct gtaggagcag gttgtttggc tggaatggca gcaggccttc
     6841 tttatacaaa agggaagatc ccaaccttgc tctcagggat tttggtgatg acttcttgtc
     6901 actcaatcat gctcttgatt atgggacgtg cgaatttagg cctgcttgga accaagcaaa
     6961 ttcaggatgt tttgcctttt gattcggatt tgaatcaact cttgacaggt ctcatctttg
     7021 tgagcattgt tattgctctc atgctctttt tcttggacac taaactcgga caagcctata
     7081 ttgctacagg ggataatcct gatatggcta gaagtttcgg gattcatact ggacgcatgg
     7141 agctcatggg cttggtctta tcaaatggtg tgattgccct tgcaggtgcc ctcattgctc
     7201 agcaagaagg ctatgccgat gtgtctcgag ggatcggggt tatcgttgtg gggcttgcaa
     7261 gtttgattat tggagaagtt attttcaaga gtttgagctt ggcagagcgt ttggttacta
     7321 tcgttgtagg ttctatcgct tatcaatttt tagtgtgggc agttatcgca cttggcttta
     7381 atacaagtta ccttcgttta tacagtgcct tgattttagc agtctgcctc atgattccaa
     7441 catttaagca aacaatcttg aaaggagcca agttaagcaa atgacagcaa ttgtagaatt
     7501 aaaaaatgca accaaaattg ttaaaaatgg ctttgatgaa gaaaagatta ttttaaatga
     7561 tgtttcctta gaaatttttg aacgggactt tatcacgatt ttgggcggaa atggtgctgg
     7621 aaaatcaacc ctctttaaca ctatagcagg gaccttatca ctaactagtg gaactatccg
     7681 tattttaggt gaagatctca ctaagttttc acccgagaag cgtgccaagt acctgtctcg
     7741 tgtcttccaa gatccaaaga tggggacagc tccccgtatg acggtcgctg aaaatctttt
     7801 aatcgccaag tttcgtggtg agaagcgtgg attgttacca cgacgcttga ctagctataa
     7861 ggatgaattt caggcaacca ttgaaaaagt aggaaatggt cttgagaaac acttgaatac
     7921 accgattgag ttcttatcag gtggacaaag acaggctttg agtctcttga tggcaacctt
     7981 gaagcgacct gaattactcc tgttagatga gcatactgct gccctggatc caaagactag
     8041 tgttgctttg atggaattga cagatgaatt tgttaagaaa gatcagctaa cagcccttat
     8101 gattactcat catatggaag atgctctcaa atacggcaat cgcttgattg tcatgaaaga
     8161 aggacggatt atccaagatt taaaccaaga agaaaaagca aaaatgaaaa tctctgatta
     8221 ttatcaactc tttgaataaa gtagggaaaa tgagtgaaat ggtttacttt ttttctgaaa
     8281 taaagtatac tatataaagt aaactatgat aacatggagg tattgtgtat ggttgacaaa
     8341 caagtcattg aagaaatcaa aaacaatgcc aacattgtgg aagtcatagg agatgtgatt
     8401 tctttacaaa aggcaggacg gaactatcta gggctctgtc cttttcatgg tgaaaaaaca
     8461 ccttctttca acgttgtaga ggacaagcag ttttaccact gttttggttg tggtcgctca
     8521 ggtgatgtct ttaaattcat cgaggagtac caaggggtta cctttatgga ggctgtccaa
     8581 atcttaggtc agcgtgtcgg gattgaggtt gaaaaaccgc tttatagtga acagaagcca
     8641 gcctcgcctc accaagctct ttatgatatg cacgaagatg cggctaaatt ttaccatgct
     8701 attctcatga caacgactat gggcgaagag gccagaaatt acctttatca gcggggtttg
     8761 acagatgaag tgcttaaaca tttttggatt ggtttagcac ctccagaacg aaactatctc
     8821 tatcaacgtt tgtctgatca gtatcgtgaa gaggatttac tggattcagg cctgttttat
     8881 ctctcggatg ccaatcaatt tgtagacacc tttcacaatc gcattatgtt tcccctgaca
     8941 aatgaccaag gaaaggtcat tgccttctca ggtcgtatct ggcaaaaaac ggattcacaa
     9001 acttctaagt ataaaaacag ccgatcgact gcaattttta acaaaagtta cgaattatat
     9061 catatggata gggcaaaaaa atcttctgga aaaactagtg agatttacct gatggaagga
     9121 ttcatggatg ttattgcagc ctatcgggct ggaatcgaaa atgctgtggc gtcgatggga
     9181 acggccttga gtcgagagca tgttgagcat ctgaaaaggt taaccaagaa attggttctt
     9241 gtttacgatg gagataaggc tgggcaagcc gcgatattga aagcattgga tgaaattggt
     9301 gatatgcctg tgcaaatcgt tagcatgcct gataacttgg atcctgatga gtatctacaa
     9361 aaaaatggtc cagaagactt ggcctatcta ttaacaaaaa ctcgtattag tccgattgag
     9421 ttctacattc atcagtacaa acctgaaaac agtgaaaatc tgcaggctca gattgagttt
     9481 attgaaaaaa tagctccctt gattgttcaa gaaaagtcca tcgctgctca aaacagctat
     9541 attcatattt tagctgacag tctggcgtcc tttgattata cccagattga gcagattgtt
     9601 aatgagagtc gtcaggtgca aaggcagaat cgcatggaaa gaatttccag accgacgcca
     9661 atcaccatgc ctgtcaccaa gcagttatcg gctattatga gggcagaagc ccatctactc
     9721 tatcggatga tggaatcccc tcttgttttg aacgattacc gtttgcgaga agactttgca
     9781 tttgctacac ctgaatttca ggtcttatat gacttgcttg gccagtatgg aaatcttcct
     9841 ccagaagttt tagcagagca gacagaggaa gttgaaagag cttggtacca agttttagct
     9901 caggatttgc ctgctgagat atcgccgcag gaacttagtg aagtagagat gactcgaaac
     9961 aaggctctct tgaatcagga caatatgaga atcaaaaaga aggtgcagga agctagccat
    10021 gtaggagata cagatacagc cctagaagaa ttggaacgtt taatttccca aaagagaaga
    10081 atggagtaat aatggcaaca aaacaaaaag aagtaacaac atttgacgta caggtagcag
    10141 aatttatccg taatcataag caaaaaggga cagcaacaga tgatgaaatc aatgcaagtc
    10201 tggttattcc ttttaccttg gacgctgatg ggattgaaga tctcttgcaa cggattcagg
    10261 atgcagggat ttctatcaca gataacgaag gaaatccaag tgcgcgtgtt cttagcaatg
    10321 aagaagaacc agaactcagc gatgaggact tgattggatc aacttctgct aaggtcaatg
    10381 accctgtccg tatgtacttg aaagaaatag gggtcgttcc tctcttgacc aatgaagagg
    10441 agaaagagtt ggcactggct gttgaagctg gcgatatcga agccaaacaa cgtcttgcgg
    10501 aagccaatct tcgtttggtt gtttccattg ccaaacgcta tgtcggtcgt ggcatgcagt
    10561 tccttgactt gattcaagaa ggaaatatgg gcttgatgaa ggcggttgac aagtttgact
    10621 attctaaagg gttcaagttt tcaacttatg caacttggtg gattcgtcag gctatcactc
    10681 gtgctattgc ggaccaagct cgtaccatcc gtatcccagt tcacatggtt gaaactatca
    10741 ataaattggt tcgtgaacag cggaatctcc ttcaagaatt ggggcaagat ccgacaccag
    10801 aacagattgc tgaacgaatg gatatgacac ctgataaggt tcgtgaaatc ttgaagattg
    10861 cccaagaacc agtatctctt gaaactccta tcggtgaaga ggacgatagc caccttggag
    10921 actttatcga agatgaagtg attgaaaatc cagtggatta tacgactcgt atcgtcttgc
    10981 gtgagcaatt ggatgaaatc ttagatactc ttacagaccg tgaagaaaat gttctgcgtc
    11041 tacgttttgg actagatgat ggaaaaatgc gcacacttga agatgtgggg aaagtcttta
    11101 acgtaactcg tgagcgtatc cgtcagattg aagcaaaggc tttgagaaaa ctacgccaac
    11161 caagtcgcag caaaccgctt cgtgatttta ttgaagacta agagtgagga aaatatggct
    11221 tatacagaag agcaaattga aaacatcaaa acacggattt taacagcctt ggaagaagtc
    11281 atcgaccctg agttgggaat cgatattgtc aatcttggtt tgatctatga gattcgtttt
    11341 gacggtgaca cagggcaaac agagattgac atgactttga caactatggg ttgtccctta
    11401 gcagaccttt tgacagacca gatttatgat gctatgatag aggttccaga agtaacggat
    11461 actgaggtta aattagtctg gtatccagcc tggacagttg aaaaaatgag tcgctatgcc
    11521 cgcattgccc taggcattaa gtaaacatat aaaagcatgt gagagactag aggaaaagcg
    11581 aggaaatttc ccctcttttc ctctagtctc tcctttcttt tgctgatttt attcaaagaa
    11641 aatgatataa tagtagttat ggagaaaaag aaattacgca tcaatatgtt gagttcaagt
    11701 gagaaagtag caggacaggg agtttcaggt gcttaccgtg aattagttcg tcttcttcac
    11761 cgtgctgcca aggaccaatt gattgttaca gaaaatcttc caatcgaggc agatgtgact
    11821 cactttcata cgattgattt tccctattat ttatcaacct tccaaaagaa acgctcaggg
    11881 agaaagattg gctatgtgca tttcttgcca gctacacttg aaggaagttt gaaaattcca
    11941 tttttcttaa agggaattgt gaaacgctat gtattttctt tttacaaccg gatggagcac
    12001 ttggttgtgg tcaatcctat gtttattgag gatttggtag cagctggtat tccacgtgaa
    12061 aaagtgacct atattcctaa ctttgtcaac aaggaaaaat ggcatcctct accacaagaa
    12121 gaggtagtca gactgcgcac agatcttggt cttagtgaca atcagtttat cgtagtaggt
    12181 gctgggcaag ttcagaaacg taaagggatt gatgacttta tccgtctggc tgaggaattg
    12241 cctcagatta cctttatctg ggctggtggc ttctcttttg gtggtatgac agatggttat
    12301 gaacactata agaaaattat ggaaaatccc cctaaaaatt tgatttttcc aggcattgta
    12361 tcgccagagc ggatgcgcga attgtatgct ctagcggatc ttttcttgtt gcctagttac
    12421 aatgagctct ttcctatgac tattttagaa gctgcgagtt gtgaggctcc tattatgttg
    12481 cgtgatttag atctctataa ggtgattttg gagggaaatt atcgggcgac agcgggtaga
    12541 gaagagatga aagaggctat tttggaatat caagcaaatc ctgctgtctt aaaagatctc
    12601 aaagaaaagg ctaagaatat ttccagagag tattctgaag agcatctgtt acaaatctgg
    12661 ttggactttt atgagaaaca agccgcttta gggagaaagt aaaaagtgag gtaatctatg
    12721 cgaattggtt tatttacaga tacctatttt cctcaggttt ctggtgttgc gaccagtatt
    12781 cgaaccttga aaacagaact tgaaaagcag ggacatgctg tttttatctt tacgacgaca
    12841 gataaggatg tcaatcgcta cgaagattgg caaattatcc gcattccaag tgttcctttc
    12901 tttgctttta aggatcgtcg ctttgcctac cgaggtttta gcaaggcact tgaaattgct
    12961 aaacagtatc agctagatat tatccatact cagacagaat tttctcttgg cctgttgggg
    13021 atttggattg cgcgtgaatt gaaaattcca gtcatccata cctatcacac ccagtatgaa
    13081 gactatgtcc attatattgc taaggggata ttgatccggc cgagtatggt caagtatctg
    13141 gttagaggtt tcctgcatga tgtggatggg gttatttgcc ctagtgagat tgtccgtgac
    13201 ttgctatctg attataaggt caaggttgaa aaacgggtca ttcctactgg gattgaatta
    13261 gccaagtttg agcgtccgga aatcaagcag gaaaatttga aagaactgcg tagtaaacta
    13321 gggattcaag atggtgaaaa gacgttgctt agtctttcga gaatctccta tgaaaaaaat
    13381 attcaagcag ttttagcagc ctttgctgat gttctgaaag aggaagacaa ggttaaactg
    13441 gtagtagctg gggatggccc ttatctgaat gacctcaaag agcaagccca gaacctagag
    13501 attcaagact cagtcatctt tacagggatg attgctccta gtgagacggc tctttactat
    13561 aaagcggcgg atttcttcat ttcggcatcg acaagcgaaa cgcaaggttt gacctacttg
    13621 gaaagcttag ccagtggaac acctgtcatt gctcacggaa atccttattt gaacaacctc
    13681 atcagtgata aaatgtttgg agccttgtac tatggagaac atgatttggc tggtgctatt
    13741 ttggaagccc tgattgcaac accagacatg aatgagcata ccttatcaga gaaattgtat
    13801 gagatttcag ctgagaactt tgggaaacga gtgcatgagt tttatctgga tgccattatt
    13861 tcaaataact tccagaaaga tttggctaaa gatgatacgg tcagtcagcg tatctttagg
    13921 acagttttgt atcttccgca acaggtggtt gctgtacctg taaaaggatc tagacgcatg
    13981 ttgaaggctt caaaaacaca gttgatcagt atgagagact attggaaaga ccatgaagaa
    14041 tagaaagagg aacagctatg aaaaaaacaa ttaatgagaa gcggtcgtga taaaaagatt
    14101 gcgggtgttt gtgctggggt ggcccattat ctggatatgg atccgactat cgttcaagtc
    14161 atttggggtg ttcttacttg ctgttacgga gctggaattg tagcttacat tattttatgg
    14221 attatcgcac cagtagcaac tgactattga aaactagctc aattcatgct aagataatag
    14281 aaaatagaat gtaacgaagg agagaaaaat ggcatttgga gataatggaa atcgtaaaaa
    14341 aactatgttt gagaaaataa ccttgtttat cgtgattatc atgctagtag caagtttatt
    14401 gggaattttt gcaactgcaa ttggtgccct cagtaatcta taaaatagat tcaagaaaat
    14461 ttagtgactg ggatttccca gccctttttt aaagtgagaa gaaataatga gtatgttttt
    14521 agatacagct aagattaagg tcaaggctgg taatggtggc gatggtatgg ttgcctttcg
    14581 tcgtgaaaaa tatgtcccta atggaggccc ttggggtggt gatggtggtc gtggaggcaa
    14641 tgtggtcttc gttgtagacg aaggactacg taccttgatg gatttccgct acaatcgtca
    14701 tttcaaggct gattctggtg aaaaagggat gaccaaaggg atgcatggtc gtggtgctga
    14761 ggaccttaga gttcgagtac cacaaggtac gactgttcgt gatgcggaga ctggcaaggt
    14821 tttaacagat ttgattgaac atgggcaaga atttatcgtt gcccacggtg gtcgtggtgg
    14881 acgtggaaat attcgtttcg cgacaccaaa aaatcctgca ccggaaatct ctgaaaatgg
    14941 agaaccaggt caggaacgtg agttacaatt ggaactaaaa atcttggcag atgtcggttt
    15001 agtaggattc ccatctgtag ggaagtcaac acttttaagt gttattacct cagctaagcc
    15061 taaaattggt gcctaccact ttaccactat tgtaccaaat ttaggtatgg ttcgcaccca
    15121 atcaggtgaa tcctttgcag tagccgactt gccaggtttg attgaagggg ctagtcaagg
    15181 tgttggtttg ggaactcagt ttctccgtca catcgagcgt acacgtgtta tccttcacat
    15241 cattgatatg tcagctagcg aaggccgtga tccatatgag gattacctag ctatcaataa
    15301 agagctggag tcttacaatc ttcgcctcat ggagcgtcca cagattattg tagctaataa
    15361 gatggacatg cctgagagtc aggaaaatct tgaagaattt aagaaaaaat tgtctgaaaa
    15421 ttatgatgaa tttgaagagt taccagctat cttcccaatt tctggattga ccaagcaagg
    15481 tctggcaaca cttttagatg ctacagctga attgttagac aagacaccag aatttttgct
    15541 ctacgacgag tccgatatgg aagaagaagc ttactatgga tttgacgaag aagaaaaagc
    15601 ctttgaaatt agtcgtgatg acgatgcgac atgggtactt tctggtgaaa aactcatgaa
    15661 actctttaat atgaccaact ttgatcgtga tgaatctgtc atgaaatttg cccgtcagct
    15721 tcgtggtatg ggggttgatg aagcccttcg tgcgcgtgga gctaaagatg gggatttggt
    15781 ccgcattggt aaatttgagt ttgaatttgt agactaggag actggtatgg gagataaacc
    15841 gatatctttc cgagatgcgg atggtaattt tgtttccgcc gcagacgttt ggaatgaaaa
    15901 gaaattggaa gaactattta atcgtctcaa tccaaatcgt gccttgagat tggcacgaac
    15961 taaaaaggaa aatccatctc agtaaagaag ctaaaaaatc ccgtgcctca tcagacacgg
    16021 gattttgtgg tacgacaggc atgtatagca aactgaatct ggaatagcac agcatatctt
    16081 ctaaaatatt gttataaatt gatttgaatt tcaaaattaa attgtttgat tcttatctca
    16141 atttgttata gtatatctga tgtcaaagtt ctcggcgagt c
//
LOCUS       DAVBHS010000035        15189 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVBHS010000035 DAVBHS010000000
VERSION     DAVBHS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15189
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.035"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(129..347)
                     /locus_tag="VQ651_000807"
     CDS             complement(129..347)
                     /locus_tag="VQ651_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753829.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(443..681)
                     /locus_tag="VQ651_000808"
                     /pseudo
     CDS             complement(443..681)
                     /locus_tag="VQ651_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(674..958)
                     /locus_tag="VQ651_000809"
     CDS             complement(674..958)
                     /locus_tag="VQ651_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753830.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            complement(1570..1740)
                     /locus_tag="VQ651_000810"
     CDS             complement(1570..1740)
                     /locus_tag="VQ651_000810"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2753831.1"
                     /translation="MTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEETG"
     gene            complement(2390..3613)
                     /locus_tag="VQ651_000811"
     CDS             complement(2390..3613)
                     /locus_tag="VQ651_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET2753832.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            3781..5103
                     /locus_tag="VQ651_000812"
     CDS             3781..5103
                     /locus_tag="VQ651_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2753833.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEDFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            5105..6991
                     /locus_tag="VQ651_000813"
     CDS             5105..6991
                     /locus_tag="VQ651_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HET2753834.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYINRVFKTDIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRGHDFVP
                     ELRFEDFLDKELSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGSNWQSVVEYLEKL
                     SSYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            7159..7503
                     /locus_tag="VQ651_000814"
     CDS             7159..7503
                     /locus_tag="VQ651_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2753835.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            7513..8925
                     /gene="lacG"
                     /locus_tag="VQ651_000815"
     CDS             7513..8925
                     /gene="lacG"
                     /locus_tag="VQ651_000815"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET2753836.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQE
                     NSPFLPDPASDFYYRYEEDITLAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGEIGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAALNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            8995..10674
                     /locus_tag="VQ651_000816"
     CDS             8995..10674
                     /locus_tag="VQ651_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2753837.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMIAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGIIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QLISFVFLSLMLVVDILIYLPFCRAYDRQLLVKEDIASSNDIILEEDTSEIMPGEIDV
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLDIQIVATRGMEYIHLTKSPSKALQFVLEHYQAV"
     gene            complement(10800..12239)
                     /locus_tag="VQ651_000817"
     CDS             complement(10800..12239)
                     /locus_tag="VQ651_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET2753838.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(12243..13592)
                     /gene="trkA"
                     /locus_tag="VQ651_000818"
     CDS             complement(12243..13592)
                     /gene="trkA"
                     /locus_tag="VQ651_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HET2753839.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            13780..14628
                     /locus_tag="VQ651_000819"
     CDS             13780..14628
                     /locus_tag="VQ651_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HET2753840.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            14643..>15189
                     /locus_tag="VQ651_000820"
     CDS             14643..>15189
                     /locus_tag="VQ651_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HET2753841.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
BASE COUNT         4650 a         2407 c         3142 g         4990 t
ORIGIN      
        1 tccgctgaaa cggctcaaac agtgctagaa attgactgga attctctaag tgatttgttc
       61 cgattttatt tcatttcatc ataaaaataa gatgactggc taattgacaa tcatcttgtt
      121 tgatactgtt aataaaattc gcgaagttta tcttgatttt ctttaaaaaa tttttctaac
      181 caatccatac cataagcata actaaaacct tctaatagaa cagagaggtc aatagtatca
      241 aatcccatgt cttttaaata tttaaaggta tcgaatggtg aatgaaagtt tttgtattta
      301 ataattgcac caatatcttg caaatcctgc tctctgatac tcatcatctt cattcctaac
      361 acatactcaa tagggactac taaaacggtc agattctcaa atgagtacaa ggattcgcat
      421 aaacttaagg gaggttgttt gttcatattt gctttgcaac atgattgttg agccataact
      481 cttcgtggat gttaagatta aactgttttc ctacacgagc aataatttca ttgattttct
      541 gattttggtc ataaaaagca tcaacatctt gtgtggcacg taaaccatga tattctaaga
      601 cataaccata attgttaaag tcagattttc ctgaattaat ttcttattta gggcttggaa
      661 gaccggtttc attttatcca tgtttgtatt tctccatcaa tttttgaaca ttggttaact
      721 tagcaggttt atttaatgaa atcacatatt tatgagttgg agtatgctct tgaaaggcat
      781 acttcaagct aattttttga aaacgggtta gttctttgga gtctagcaac ttttctacat
      841 cttggtttgt taaataagaa tagtcttcaa aaataacttt tcgcaaatag cgaaaccaat
      901 ggcttgcgtt gaattgtgat tgtttggatg cctgtatcat ataatcgtac caatccattg
      961 tcttcctcct ataaaattta gtttatatta ttgtagttta tattcaaaaa tgtagttttt
     1021 cttaattagc tcgtatctat aggatgatac tgaaaatctt cttgttgctt taaatcacaa
     1081 tcgaaaaatt ctgtttttca tcttagaaaa gaatgtttag aaatgtaaaa tgaacctttt
     1141 ttcttatccg aaaaccttac taaagtatat aatttaggca gtcaattctg tatgataatg
     1201 tttgtactat cgtcgagttt ttgcttgatt gcctaacgag ataaatttta gaagagtttt
     1261 cggtgtgatt tgacaagttt ctgactggaa ggatagttac actagtattg tttattaaaa
     1321 tgagcagata gagaaaattt ggaatctatt tgcttattat caatcgtttt tttagcttat
     1381 ttgtcttaga ttggtataga actgagctat ggattaatct tcttctatct tatcctgttc
     1441 ctaaatatgt tggtagaaaa atgaagcagt tgttacgagt gtctgtattg ggaaaaattt
     1501 tgtctataga attctttaaa tgatatgaag gctaggaatg ccaaaacact gacatatttc
     1561 gtttaaagtc tatccagtct cttcaattgt atttgtagaa atgctatcag taatatattc
     1621 gcgaacacta tgatttacaa cagtgtggtc gtacatcttt gtaactttat tatacaacaa
     1681 ccatttacag gtgtaaagtt gagcagtgtc tttagtaaat ttagggactt gttttgtcat
     1741 agttttgata cctgtattcg ttgtttcatg agcatttgac gtttcttttc atgtaggcat
     1801 ttcaacaatt agaatgatat ttttccgagg atgaacttat atattgtgac tttttagagt
     1861 tatgatgcct cttgatttga ctaccgctat caatttcatc cacttttaca gtttcatggt
     1921 tttatgaacc gaaacatcta ggtacaggtt ttagcatcaa ttgattgttt ttgagccata
     1981 tgaagttgaa acgtggagat gcttttcttg tgataacgcc ctattcaaag aggaataacc
     2041 gtttttggag agagttcagt actatgtggc gctaaatact atatgtaaaa catatattaa
     2101 acaagattta actaaggaga attgtaaaaa caaaaatgag aatttcatat aaagtttatt
     2161 ctaggagtat gtatcttgaa taaaaatcgt tatttaagtt cagggttatg atgaatgttt
     2221 ttataataat aaaatgcagc tagaagaaaa gttattttaa tataaatcac tcaacataga
     2281 gagaaggcgg tttcctatat ggaagtcgtc ttgttttcat attgtctaaa tacgagatat
     2341 ctactacagg ttatagttga tttaaacagg tctatagatt tctaatatat taaggtgaaa
     2401 taatatcttg taatacattg ctatgttcta tgaaaaaagc gacgatagct aatgcacaag
     2461 ctcctatact tccacgatgt ttgttgtaag gtaaaatttc aatttctata ttacgagtaa
     2521 agggaacgaa gtggagctgg tcttggattt tatccctgac ttcatgagtg aaaggttgat
     2581 aattaagcaa ttgactgttg atatagattt tatcagcagg attgatgagg aggagatttg
     2641 caatagaagt ggctaataaa ttgactcctt tatcaatttg ttgacgtaaa gcggagtcgc
     2701 ctaaattata agccgttaaa agggtgtcta aatgaatgtc tttttcagtc tttacaaggc
     2761 tttttagtac agttagttgg gaatttttaa ataataattg ggcgtgtttg attaaccaag
     2821 catccgaaat atatgtttgt aaacaacctt tttttccgca ttcacaatat tgcccattca
     2881 attcgacaat ggtgtgtcca atttctccga tataaggatt tttagaggct cctatttttt
     2941 cttttgtgaa aaaggaagtg taaataccta atccagcgtg taggaaaata aagttatcgg
     3001 gggtattgtg tggattaaaa aggtattgtc ctatagccat acaatcgata ttatttttta
     3061 caataaaagg aaaattgaat cttttaatta catttaataa attaaaagat tcccatatgg
     3121 ggttgtttgt aatgatactt ccggagtctt tgttataatg tccaggtatt gataagccaa
     3181 atccaagtaa ttttgtttcc aaagggacgt actctatagc tgtttttatc atattggtaa
     3241 tggtggaatt gatttctgaa ataggataat cttcattcaa aatttctgtc tcgaaaagag
     3301 ttttcccttg taagtcacat agactgagaa agatacctag gacgtttatt tcaatacctc
     3361 caacaacata cggaatatta tctgtgatag aaatagtttt tctacgtctt ccagatccgg
     3421 aaaactcgct aaattcatca ccagtttcgt atatgaggga ctgttcgctt agctctttta
     3481 tgatgttagt cgttgttgct ggagtaagtc ctgtttcatt aacaatatct gtacgggatg
     3541 ttgctggatt tcgatataga aactccagta tagtggctct attttgtaaa aaggctgatt
     3601 gttttgtaat catttgtttc ctcctttggt ttattttata ttaaacataa gaaaaaatca
     3661 attaaaatta ttattttata caaagaaaaa aatattgtaa aaacaacaaa aaatatattg
     3721 acaacgcttt ctgttagtgg tatatttaat ttataaaaaa taaaaaaagg agttccggtg
     3781 atgaacaata ttttagcgtt tttagaaaca aaagtcgctc cgtttggtga aaaagttggc
     3841 aaccaacggc atttgaaagc tattcgtgaa ggatttatga tggcaatgcc tttgatttta
     3901 gtcggatctt tatttcttat tctaatcagt tggcctcaag aggattttac aaattggctg
     3961 aatagtgttg gattgctaag tatcttgaca actatgaatc agtcaacagt agcgattatc
     4021 tccttggtcg cttgtttcgg tattgcctac aggttgtcgg aaggatatgg tacagatggt
     4081 ccgtcggcag ggatcatagc cttatccagt tttgtattga tggcacctcg tttttcgagt
     4141 atggtttatg ataaaaatgg ggagcaggtc aagcagttat ttggcggcgc aataccattt
     4201 tctagcctga atgcatcttc tttgtttatg gcgattacta ttggattggt tacagcagag
     4261 atttatcgta tgtttatcca gcgcggaatt acgataaaaa tgccaagtgg tgtcccagat
     4321 gtggtaagta aatcattttc agctctttta cctggcttta ctacttttgt tttgtgggct
     4381 ctggtcttaa aaggtcttga agcggcagga gttgcaggag gtctcaacgg actcctaggt
     4441 gcaattgttg gaacaccgct taagttaatt gcaggaacgc ttccaggtat gattctatgt
     4501 gttattgtaa actcattctt ttggttctgt ggagttaatg ggggacaagt tttaaatgct
     4561 tttgtagatc cagtttggtt acaatttact acagaaaacc aagaagctgt ggctgcagga
     4621 caaacactcc aacacattat tacattaccg tttaaagatt tatttgtatt tattggtggc
     4681 ggtggagcga ctattggtct tgcgatttgt ctcttcctat ttagtaagag tcgtgcgaat
     4741 aaaacattag gtaagctagc tattataccg tctattttta atatcaatac agctattcta
     4801 tttacgtttc caacagtttt aaatccgatt atgctgattc cgtttattgc tactcctaca
     4861 atcaatgcct taattaccta tgtatcaatg gctgtaggat tagtacccta cacaacaggt
     4921 gtaatccttc cgtggacaat gccaccgatc ataggaggct tccttgcgac aggagctagt
     4981 tggcgaggag ctctattaca agttgttttg attttggttt ctgtagcaat ttattatcca
     5041 ttcttcaaaa ttgcagataa acgcaatctt gaaaaagaaa aagctactgt tggagggaaa
     5101 taagatggtt atcagagtat ttgatcagca gaaaaatact tattctagct ttgccttaga
     5161 ggaattaagt tactatataa atcgggtctt taagactgac atagaacttg tcgaggagaa
     5221 ggaagcggat atttttgtag gattagtcaa taaagaggat agaaaagacc atgttcttat
     5281 ctcattagac aagggtaagg ggagaattga gtctaataca attgtaggtt tgcttattgg
     5341 aatttaccga atgtttcatg aatttggggt tgtgtatact agaccagggc gcggacatga
     5401 ctttgttcca gagttacgat ttgaagattt tttagataaa gagctatcta tagatgaaac
     5461 agccagttac tatcataggg gagtatgtat agagggagca gattcatttg aaaatatact
     5521 agatttcatt gattggctac ctaagattgg gatgaacagt tttttcattc agtttgaaaa
     5581 tccctattct tttttgaaac gttggtatga acatgagttt aatccatacc taaataaaga
     5641 aaaattctca aatgaattag tacaagaatt gagtgatagg ttggataaag aattgcaaaa
     5701 aagaggtctt attcatcatc gtgttggtca tggatggaca ggtgaggttt taggttactc
     5761 ttcaaaattt ggctgggaat caggtcttag tatttcagag gagaagaaac cctatgtcgc
     5821 tgaaataaac gggaaacgag aattatttaa tacggctccg attttaacca gtctggattt
     5881 ttcaaatcca gatgtggcgg ataagatggt agaaattatc aaggattatg ccaagaaaag
     5941 acctgatgtt aactacttac atgtatggtt gtcggatgct cgtaataata tttgtgaatg
     6001 cgaaaactgt agacaagaat tggtttcgga tcagtatatt cgtattctca atcaattgga
     6061 tagggcttta acgagtgagg gattagatac aaagatttgt tttctgcttt accatgagtt
     6121 gttatgggcg cctcagaaag aaaaattaga taatcctgaa cgctttacca tgatgtttgc
     6181 accgattaca agaacatttg aaatgagtta tgcagatgta gattttgaca attccatacc
     6241 tacgcctaaa ccttatctgc gtaataaaat tatacttccg aattctcttg aggaaaattt
     6301 atcttatctt tttgagtggc aaaaaacatt taaaggagat agtttcgtat atgactatcc
     6361 tttagggcgt gctcattatg gcgatttagg ctatatgaaa attagtcaaa ccatttacag
     6421 agatgtatct tatctttcta acctacattt gaacggttac atttcgtgtc aagaattacg
     6481 tgccggattc cctcataatt ttcctaatta tgttatgggg gaaatgctct ggaagaagac
     6541 aagaagttat gaagaattga ttgaagaata cttttctgct ttgtatgggt caaattggca
     6601 gtctgttgtt gaatatttag aaaaattatc cagttattcc tcatgtgatt attttaatgc
     6661 aattggcagt cgtcaaaatg atgttttagc gaatcattat tatatagctt acaatctagc
     6721 tgataatttt ttgccaatta ttgaggaaaa tatttctaag ttattaaata gtcaaaagga
     6781 tgaatggaaa cagctcagtt atcatcgtga atatgttgtt aagatggcga aggctttata
     6841 tcttcaagca accggaaaaa caaggcaagc tcaagatgaa tggagaaatg tgttgaatta
     6901 tatccgtggg cacgaattgc tatttcaatc taatttggat gtttatcgtg taattgaagt
     6961 agcaaaaaat tacgctggtt tccacttata aatcataagt atagaaaatg aaccaaggta
     7021 ttcagagaag attgatccta aatattatga aatttaagga tttttaagat atttagggtc
     7081 gactttctat ttatatcgta gcgaagtcat tttaataatg atgtgtaaaa gatggatcaa
     7141 gattgaggag gaagaaagat gaaatcaaaa gaagaaataa atatgcttgg ttttacaatt
     7201 gtcgcttacg caggagatgc aaggtcagat ttgatggatg ctttggcgtt tgcgagagat
     7261 ggatattttg aacaggcaag agaattggtt gagtctgcaa acgactcaat agtgtctgcc
     7321 catcgagaac agactaattt attagcggag gaggcatctg gagataattt tgaagtgagt
     7381 tttatcatga ttcatggtca agataccttg atgacaacga tgctattgta tgatcaggtc
     7441 aagtttttta ttgatgaata tgaacgaatt cgaaagattg aagaacatat tggtttgcaa
     7501 tgaggattag tcatggaaaa tttacaggtt aaagccttac cgaaggagtt tttattagga
     7561 gctgctaccg ctgcttatca agtagagggt gcaactaggg tagatggcaa aggaacaaat
     7621 atgtgggatg tttatttgca agaaaacagt ccgttcttac cagatccagc tagtgatttt
     7681 tattatcgtt acgaagagga tataactttg gcggcagaac atggtttgca ggctttgcgt
     7741 ttatctattt cttgggttcg tatatttcct gatatagatg gggagcctaa tatattagct
     7801 gttcgttatt accatagagt ctttcagtct tgcttaaaac ataatgtgat tccgtttgtt
     7861 tctttacatc attttgattc gcctcagaaa atgttagaaa caggggattg gttgaataga
     7921 gagaatattg atcgtttcat acgatatgct cgcttttgtt tccaagaatt tacagaagtc
     7981 aagcattggt ttacaatcaa tgaactgatg tctcttgctg caggtcaata tataggaggt
     8041 cagtttcctc caaatcatca ttttcaatta tctgaagcaa ttcaagcgaa tcataatatg
     8101 ttgttggcgc atgctcttgc agtcctcgaa tttcatcaat taggaattga gggagagata
     8161 ggttgtattc atgctttaaa gccaggctat cctattgatg ggcaaaaaga aaatattttg
     8221 gcagctaaac ggtatgatgt ttataataat aaatttctat tagatggaac ttttttgggc
     8281 tactacagtg aggacacgct ttttcacttg aatcaaatat tggaagctaa taattctagc
     8341 tttattattg aagatggtga tttagaaatt atgaagagag ctgcagctct taatacgatg
     8401 tttgggatga attattatcg ttcagaattt attcgtgaat acaaaggtga aaatagacaa
     8461 gaatttaatt caacaggaat aaaaggacag tcttctttta aattaaatgc tctaggtgaa
     8521 tttgtaaaaa aacctggtat tccgacaaca gattgggatt ggaatattta tcctcaaggg
     8581 ttatttgata tgttgcttcg tatcaaagaa gaatatcctc aacatccggt catttattta
     8641 actgaaaatg gtacagccct taaagaagtt aagccagagg gcgagaatga tattattgat
     8701 gacagtaaga gaatccgtta tattgagcaa catttacaca aagttttaga ggctcgagat
     8761 agaggagtca atattcaagg ctattttata tggtctttgc aagatcaatt ttcttgggcg
     8821 aatggctaca ataagcgata tggtcttttc tttgttgatt atgaaacaca gaagagatat
     8881 attaagaaaa gtgctctttg ggtaaaaggg ctaaaacgga attaaggtta gcgatttgac
     8941 tgatgtttaa tatattttaa ctatgagctt aaatttttta caggaggagt ttttatggat
     9001 aagttagtcg ctgccattga aaagcaacaa gggaaatttg aaaaaatttc tactaataat
     9061 tatatgatag ctattaaaga tggatttatt gctactatgc ctttaattat gttttcaagc
     9121 tttttgatga ttattattat gattcctaaa aatttcggag tagagttacc gagtccagct
     9181 attgcctgga tgaggaaagt gtatatgcta accatgggag ttttgggtat tattgtttca
     9241 gggacagttg caaagtcatt ggctggaaat gttaatagaa aaatgcctcc tggaaaggta
     9301 ataaatgata cttctgcaat gttggcggcc atatgtggct atcttgtatt aactgtaacg
     9361 cttgtagttg atgagaaaac gggatctaca agtttgtcga caaactattt aggatctcaa
     9421 ggattgataa cttcgtttgt cagtgccttt attactgtaa atgtttaccg attctgtatt
     9481 aagcgagaca ttactattca tttacctaag gaagttcctg gggctatatc acaagctttt
     9541 agagatattt ttcctttttc ttttgtttta cttattagtg gtttgttaga tattgtatct
     9601 cggtttagtt tagatgttcc ttttgcccaa gtatttcaac aactattgac tcctattttt
     9661 aagggggcag aatcatatcc tgctatgatg ttgatttggt ttatgtgtgc tttgctttgg
     9721 tttgttggaa ttcatggacc atctattgtc ttacctgctg ttacagcttt acaactgagc
     9781 aatatggaag agaatgctca acttcttgca aatgggcagt tcccttatca ttctttaaca
     9841 cctaatttcg ggaattatat cgctgctatt ggaggaacgg gggctacctt tgttgtacca
     9901 tttattttga ttttctttat gcggtctaaa caattaaaat cggtaggtaa agctacaatt
     9961 actcctgttt tatttgcggt aaatgaacct cttctatttg gtatgcctgt tattttgaat
    10021 ccctatcttt ttgtcccttt tttgatgact ccaccagtga atgtatttct aggaaaggta
    10081 tttattgatt tctttggaat gaatggattt tatatccagt taccttgggc ctttcctggt
    10141 cccttgggat tgttaattgg aacgaatttt caacttatct cctttgtatt tttatctttg
    10201 atgttagttg tcgacatatt gatttatttg ccattctgta gagcgtatga tagacagtta
    10261 ctggtgaaag aagatattgc aagctcaaat gatattattt tagaggagga tacaagtgaa
    10321 ataatgcctg gtgagataga tgtaataaaa agtaaggagt tgaaagtact ggttctttgt
    10381 gcagggtctg gaacaagtgc gcaattagcc aatgcaatta acgagggcgc tcaattagca
    10441 gaagttagag tgattgcgaa ttcaggagcg tacggagctc attatgatat tatgggtgtt
    10501 tatgatttaa ttattctggc cccacaagtt cggagttatt atagagagat gaaggtggat
    10561 gcagaaagat tagatattca gatagttgct accagaggaa tggaatatat tcatttaaca
    10621 aagagtccaa gtaaagcctt acaatttgta ttggagcatt accaagctgt gtagtaagtt
    10681 tttccatctt ttatttgagt aaagattttg cttacagata ggcttggatt taaaaacgtt
    10741 cccccttttt taatataaga atccctcttt cacaattgta aaaagaggga ttttgtattt
    10801 tatctcttag accaagttct cttcataaag agaaggagga ttgggtaaat ctccaagcgc
    10861 cctgcaatca ttgcaaagga taggagaatt tttgagatgg gactaaagat tgagaaacta
    10921 gaagtggttc ctagaatagg cccgatatta ttgaaacagc taaagacagc gctggtcacg
    10981 accagaaaat cattgctatc taggctgaca ataaagataa gcgctagcaa aatcatagca
    11041 tagatgacaa agtacttgag aatcttatgc tgggtatctt tgtcaatcac cgttttatta
    11101 acatggaggg tcaaaacacg gtggggcgat aggattgaca aaatttggtt tttggcaatt
    11161 tttgaaagga tgaggcctcg aataatcttg agtccacctg cagttgatcc agcagagcca
    11221 ccgattgcca tgaggaaaag gaggataaac tgggagaaga ggggccagtt ggtaatatct
    11281 ccatatccaa aaccagttgt tgtaatgatg ttggaaacct ggaagaaggt catttcaaag
    11341 ctctttgaaa accctgggta gaggtagagg gtgttgaggc taatcaagcc tgtagaaacc
    11401 agtacaatga ccaagtaagc cctaagctct tcatctccaa agaaggcctt gatgcgacgg
    11461 agcatgaggt agtagtagag gttgaaattt actccaaaaa ccagaactcc gatactgacc
    11521 agataggtaa tcagtgagct gccatagtgg gcaattccgt cgttatagac ggtaaagcct
    11581 ccagttcccg ctgtccccat agcaatgaca aaactatcgt agagaggcat accagctaga
    11641 taatagatga tgacaaagag ggagaagaga gctagataaa ggagatagag aatctgggca
    11701 gtgtttttta gtttggatac aaccttgcca aaaacaggac ctggaacctc agccttcatc
    11761 acctctaggt ggctattttt ggcattgtcc ataatagcaa gtgcaaaaac aagcactccc
    11821 atccctccaa tcaagtgggt aaaacttcgc cagaagagga gggaacgact gagaactgaa
    11881 acgtcgttca aaatacttgc tccagtagtc gtaaagccgg aactaatctc aaaaaaggca
    11941 tcaataaggc tggggatttg cccagaaaag acaaagggga gaccaccaaa gaaagaccaa
    12001 aggatccaac agagggcaac gatcaagact ccctccttgg cataaatccg ttgatttttt
    12061 ggcttctgta aactccctga accgcctaac aatacgagaa tccctatggt cgaaaagagg
    12121 gctgtaaaga cttggctcga ttcacggtaa tagacagcga tagccacagg aaccaagagg
    12181 agaacagctt caatcaaaag taactttgaa aggaggtaac gaatcatact tttattcatt
    12241 tcttacctcg caatcaagtc ataaatcttg gtgatgtttg gcaacaaggt tgttactagg
    12301 agcttgtctc caacttccaa catatcctcc ccagttggga aaatagtctt gccctttcga
    12361 ataatggctg caataagaac cccttttttc aatttcagtt gagaaagagg tttggcagtc
    12421 attttattgg cttccttgat atggaattgc agggtttcga tttggccatt ggctagatgg
    12481 tgcatggctt gaaggtctga atactgggca ttaactcgac cacgaataaa gtgcataatc
    12541 gtatctacag cgatgctttt aggtgtgatg atacttgaaa aatcaggcgc attgataatc
    12601 tcgaggagac tggtacgatt gaccttagta atatttttct gtacacctac cctgtcaagg
    12661 aacatagatg taatcagatt ttcctcatcg actcctgtta gagtcgcaac ggcatcatag
    12721 tgttgagcac tttcttccag caggatatct tttgcggttc catctccttg aacgatgtag
    12781 agatttggga atttctcgct aaagaagctg gcgatttcag gattgatttc aatgactttt
    12841 gtatcgatac gactatcttt gagaatacca agtagataat aggcaattct acctgcccca
    12901 acgatgagaa ggctcttcac ggcgcgtgat ttaaaataat tatggaagag tatcatatcg
    12961 acacggttac cagtgacaaa gattctatct ttatcctgta cagtcatgtc accgcttgga
    13021 atgataattt gatgatccct ctctatcgca cagacaatga cattaccaaa ttttttacga
    13081 aaatcagaaa tgggcatttg gcaaagaccg ctggtggact tgacgacaaa ttccatgagg
    13141 ctaacgcgtc caccagcaaa gcgttcgaca gacagggcgt tggggaagtc aatgatattc
    13201 gcgatagcac gggcagccaa gagctcagga ttaacgataa gagaaaaacc gagaatattc
    13261 ttttccttga aataagagtt agaatattca gggttccgca cccgaacgat agtttcttta
    13321 gctcccattt tcttggctag aactgctgca atcatgttga cttcatcgtg ctcagtcagg
    13381 gcgataaaga tatcacaatc ttggacgctg gcttgctcaa gaatggcaaa atcggccccg
    13441 ttaccaagga taccaatgat atcaaagcga ctgacaatat gattgagaac agcttcgtct
    13501 tgctcaatca gcaaaacatc atgcttttct gcaaccaagg agcgacagag ggcaaaacca
    13561 acttttcccc ctccgacaag gataattttc ataataaaac ctactttttc atgatgtaac
    13621 tatcataccc tttttcaaga aaaaatgcac ctactagcta ataacaagag cttttagtga
    13681 aaattcgcta taagataaaa ctatacccta accaattgaa atagctatta gcgactttct
    13741 ctgaaatatg gtatgataaa ggatatacaa ggagataaaa tgaataataa tttactggta
    13801 ttacaatcag actttggtct ggttgatggt gcggtatcgg ctatgattgg agtggcttta
    13861 gaagagtctc caaccttaaa aatccatcac ttgacgcacg atatcacgcc ttataatatt
    13921 tttgagggga gctatcgtct ctttcagacg gtggattact ggcctgaggg aacgacgttt
    13981 gtatcggttg tcgatccagg tgtcggttcg aaacgtaaga gtgtagttgc caagactgca
    14041 aaaaatcaat acattgtcac gccagataat gggacgcttt cctttatcaa gaaacacgtt
    14101 ggcattgtag ccattcgtga gatttctgag gtggccaata ggcgtcaaaa cacagagcat
    14161 tcttatacct tccacggtcg tgatgtctat gcctatactg gtgctaaact ggccagtggt
    14221 cacattactt ttgaggaagt agggccagag ctcagtgtgg aacagattgt agagcttcca
    14281 gtcgtagcga ccatcataga agatcatctg gtgaagggag ccattgatat tctggatgtg
    14341 cgtttcggtt cgctttggac ctctatcaca cgggaagaat tttacaagtt ggaaccagaa
    14401 tttggtgatc gttttgaagt gaccatctat catgctgata tgctggtcta tcaaaatcag
    14461 gttgtctatg gcaaatcatt tgcagatgtg agaattgggc aacccatcct ttacatcaac
    14521 tctctctatc gtttaggttt ggctatcaac caaggttcct ttgccaaggc ctataatgta
    14581 ggtgtcggtt catcttggac cattgaaata aagaaaatag aaggataaaa taggagaata
    14641 gtatggaaat caaatttaca attaaacaag ttgttgctgt cggaattggc gctgccctct
    14701 ttgtcgtcat cgggatgatc aacattccaa cccctgttcc aaatacaagc atccagcttc
    14761 agtatgcggt acaagcgcta ctttctatta tttttggacc gattatcggt ttgcttgttg
    14821 ggttgattgg tcatgcaatc aaggactctc ttgttggtta tggtctatgg tggacttgga
    14881 ttatcgctag cggactcttt ggtttagttg tgggactttt tagaaagtac gttcgcgtga
    14941 tcaatggtgt ttttgactgg aaagatattc ttatttttaa cctcattcaa ctacttgcaa
    15001 atgcccttgt ttggggtgtc ttggcaccac ttggagatgt tgtgatttat caagaagcgg
    15061 cagaaaaagt atttgcacaa ggaattgttg cgggaattgc caatggtgta tctgtagcta
    15121 ttgcaggaac tcttctctta cttgcctatg caggaaccca aactcgtgca ggaagtttga
    15181 aaaaggact
//
LOCUS       DAVBHS010000036        15106 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVBHS010000036 DAVBHS010000000
VERSION     DAVBHS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15106
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.036"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(123..1931)
                     /gene="glmS"
                     /locus_tag="VQ651_000821"
     CDS             complement(123..1931)
                     /gene="glmS"
                     /locus_tag="VQ651_000821"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET2753842.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(2123..3502)
                     /gene="bglA"
                     /locus_tag="VQ651_000822"
     CDS             complement(2123..3502)
                     /gene="bglA"
                     /locus_tag="VQ651_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET2753843.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIHDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KKLSDNNGF"
     gene            complement(3602..5455)
                     /locus_tag="VQ651_000823"
     CDS             complement(3602..5455)
                     /locus_tag="VQ651_000823"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET2753844.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(5468..6727)
                     /gene="rseP"
                     /locus_tag="VQ651_000824"
     CDS             complement(5468..6727)
                     /gene="rseP"
                     /locus_tag="VQ651_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET2753845.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(6749..7552)
                     /locus_tag="VQ651_000825"
     CDS             complement(6749..7552)
                     /locus_tag="VQ651_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET2753846.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(7561..8319)
                     /locus_tag="VQ651_000826"
     CDS             complement(7561..8319)
                     /locus_tag="VQ651_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET2753847.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(8550..9122)
                     /locus_tag="VQ651_000827"
     CDS             complement(8550..9122)
                     /locus_tag="VQ651_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET2753848.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(9103..10074)
                     /gene="ruvB"
                     /locus_tag="VQ651_000828"
     CDS             complement(9103..10074)
                     /gene="ruvB"
                     /locus_tag="VQ651_000828"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET2753849.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            10235..10327
                     /locus_tag="VQ651_000829"
     CDS             10235..10327
                     /locus_tag="VQ651_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HET2753850.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            complement(10954..11370)
                     /locus_tag="VQ651_000830"
     CDS             complement(10954..11370)
                     /locus_tag="VQ651_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2753851.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(11382..12077)
                     /locus_tag="VQ651_000831"
     CDS             complement(11382..12077)
                     /locus_tag="VQ651_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2753852.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            complement(12271..14772)
                     /locus_tag="VQ651_000832"
     CDS             complement(12271..14772)
                     /locus_tag="VQ651_000832"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET2753853.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSTEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLIRSKEIVAENNGALD
                     KVYNETVKAVTEQIDSLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAATGESISYVTWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
BASE COUNT         4368 a         3501 c         2877 g         4360 t
ORIGIN      
        1 tacctacggc aaggcgacgg tgacgtggtt tgaagagatt ttctaagagt ataacacgaa
       61 tcttgagtac aagcaaccaa tttgctcaca agaaaaagcc tagataacta gactttttta
      121 gcttattcta ccgttactga cttggcaagg ttacgtggtt tgtccacatc gaggccacgg
      181 tggagggttg caaagtaagc gactaattgc gttggtacga ccattgaaat tggtgagagg
      241 tatggatgta cggtcgtaag gacgatatcg tcggtatctt tggctacatt ctcttctgcg
      301 atagtgagga ctttggcacc acgggctgcg acctcttgga tatttccacg agtatggttg
      361 gcaagaactg gatctgacaa gagagccaaa acaggcgttc cttcttcaat caaggcaatg
      421 gttccgtgct tgagttctcc tgccgcaaaa ccttcacact ggatataaga aatctctttg
      481 agtttgagac ttgcttccat ggctacgtaa taatcttgac cacgtccgat gtaaaaggcg
      541 ttacgagttg tttcaagaag ttcacgaacc ttggcttcaa tggtttcttt ctctgaaaga
      601 gttgattcaa tagactgagc tacgattgac aattcatgaa ccaggtcaaa ggcttgcgct
      661 ttagcattac catttgcttc tccgactgct tttgcaagga aggcaagggc tgcgatttgc
      721 gctgtatagg ctttagttga tgccacggca atttcaggac ctgcgtgaag gagcatggta
      781 tagttggctt cacgtgagag ggttgaacct ggaacatttg tcactgttaa gcttggaatt
      841 cccatttcat tagccttgac caaaacttga cgactatccg ctgtttcacc agattggctg
      901 ataaagatga agagtggttt cttgctgaga agtggcatac cgtagcccca ctcagatgag
      961 attccaagtt caactggtgt atctgtcaat tcttccaaca tcttcttaga agcaaatcct
     1021 gcatggtaag atgttccagc tgcaaggatg tagatgcggt ctgcgtcttg aacagcctta
     1081 atgatatctg ggtctacgac aacttgacca gcctcatctg tgtaggcttg gatgagtttc
     1141 cgcataacag ttggttgctc gtcaatttcc ttgagcatgt agtaagggta agttccctta
     1201 ccgatatctg acaagtcaag ttcagcagtg tagctagcac gctcacgacg atttccatca
     1261 tagtcttgaa cttccacgct atcagccttg acgattacca actcttggtc atgaatttcc
     1321 atgtattggt tggtttcacg aatcatagcc atagcatctg agcagaccat attgtagcct
     1381 tccccaagac caatcaaaag tggagatttg tttttcgcta catagatgac atctggattt
     1441 tcagagtcaa tcaaggcaaa ggcatatgaa ccacggataa tatgaagagc ttttttaaag
     1501 gcttcaagaa ctgagagacc gtcttcttcc gcaaattttc caatcaaatg tacggcgatt
     1561 tccgtatctg tttgcccttt gaagtggtgc ccagcaaggt attcttcttt aatttcaagg
     1621 tagttttcaa tcaccccatt gtgaaccaag acaaaacgtt ctgtctcaga gcggtgtggg
     1681 tgagcattgt cttccgttgg tttcccatga gttgcccaac gagtatgtcc gataccagtt
     1741 gttccctcaa caccagctgt cttggcagac aattctgcga tacgaccaac agccttcacc
     1801 aaatggttat cagcaccatc taggacaaaa attcccgcag aatcatagcc acggtattca
     1861 agcttttcaa gcccttgaat caaaatatca gttgcatttg tgtttccaac aacaccaaca
     1921 attccacaca tagtatatac gacacaggca agctgtgctt tctccttaaa attggtatag
     1981 tctaattcat cttttataga atcagcaaaa acagtatata cttgtttctt tcacttgtca
     2041 agagtaaaaa ttggtatagt tcaaattaag ctcctgtaag caaaaaactc tgaccgattg
     2101 ggataatcag tcagagtcct ttttaaaatc cattattatc gcttaatttt ttgaaccagt
     2161 ggcctgattt cttcagacga cgttcttgcg tttccaagtc taattcgacc aaaccatagc
     2221 gatttttata gctgttgagc catgaccagc agtcaataaa ggtccaaatc aagtagccct
     2281 tacagttggc accatcttca atggcacggt gaagttcacg aagatgacct tttacaaagt
     2341 caatacggta atcatcttga atcattccat cttgacggaa tttttcttcc ccttcaacac
     2401 ccataccatt ctcagtcaac atccattcaa tattgccata attttccttg atattttggg
     2461 cgatgtcata aatcccttgc tcataaatct cccaaccacg gtgagaattg attttacgtc
     2521 caggcatcac ataaggctcg taaaaatgtt ctggtaagag tggactctct ggatgcttag
     2581 caaatcgagg agccataaca cgcaaaggtt gatagtagtt cacaccaagg aagtccaccg
     2641 tattatcatg aatgagttcc aactcttcct ctgtagcatc aggtaaaaga ccgtgttcat
     2701 gcaagatttc taccaactcc tgtggataag tccccaagac agatggatct aagaaagatt
     2761 gggcctgaaa aagggccgca atacgagctg ccttgacatc agcaggatgc tggctacgtg
     2821 gataagccgg tgtcaagttg aggacaatcc caatcttgga atcaggcaaa agttcatggc
     2881 aagccttaac agcccggctg ctggccaatt gtgtatgata ggctacctta acagctgcct
     2941 ctgcatccac cttatgtgga taatgggcat cataaaaata accaaattct acaggaacga
     3001 tgggctcgtt aaaggtaatc cattgatcca ctaaatctcc ataagtctca aaacaaaaac
     3061 gagcatagtc ttcataggct gagactgtcg ccttattttc ccaaccatca ccatcctctt
     3121 gaagggcaaa aggtaaatca aaatgataga gattgactaa cagacgaatt cctttagcct
     3181 taatagcctc aaagacctta cgataaaaat ccacaccttg agggttgact tttccacagc
     3241 cttgtggaaa aatccgtgac cactgaatag aagtccgaaa gactgtgtga ccagtctcta
     3301 acaaaagctc aatatcccgc tcccaatttt cataaaaagt cgatgtctta tctgaaccaa
     3361 tcccattata gtaacgattt ggctccactt ggaaccagta atcccagaga ttgtctccct
     3421 taccgtcacc agctacacgt ccttctgtct gcggtccaga agtagaggat ccccagacaa
     3481 aatcctttgg aaatcttagc ataacattta cctctttatc tactcatttc tcccattata
     3541 cagaaaaaac aaggtaaaaa ctagttacat tttttccttg tttttcttct gattatagtt
     3601 tttatttctt gcttaggatt tcaagcgttt caagcacgtt atctgcatga acctcaatgg
     3661 tgtcaccagt tgccttgatc ttaacttcta caatgccatc ggccgctttt ttaccaacag
     3721 tgatacggat tggaagacca atcaagtcac tatcgctaaa tttaacaccg acacgttcgt
     3781 tacggtcatc tgtcaagact tcataaccag ctcccatcaa gcttgcttca agtttttctg
     3841 tcaaggcttg cgcttcttca tccttgacat tgacagtaat caaatgcaca tcaaatggtg
     3901 ccaattcttt agggaaattg attccccaag cgtaacggta ttcacctttt ggcgttttgt
     3961 taacaaagag gcgagcgtgt tgctccatca ctgctgaaag aagacggctg acaccgatac
     4021 cgtaacatcc catgatgatt ggcacagcac gaccattttc atccaagaca tctgctccca
     4081 tgcttgctga gtagcgagtt ccgagtttga aaatatgacc gatctcaata ccacgcgcaa
     4141 agttaaggac accttgtcca tctggggaaa tttcaccctc acgaacttca cggatatcca
     4201 catattctgc agtaaaatca cggcctgggt tcacaccagt caagtggtag ccatcttcgt
     4261 tagcaccgac aactgcattg cgaacatctt gcaccttacg atctgcaata attttaatat
     4321 tctctggcaa accaactggt ccaagtgaac caaatcctgc ttgaacaaca ttcgccactt
     4381 cttcttcgct agcaacgtca aagaaatctg ctcctaagtg gtttttcaac ttgacttcgt
     4441 tgagttggtc atttccaact agaagggctg caacaagctc accatctgca atgtagaaga
     4501 gggttttaat cgtttgttct tctggaacat tgaggaaagc tgcaacttca tcaattgatt
     4561 taacatctgg cgttgcaaca cgagtaactt cttcttcagc gacaacacgg ttgcttggtt
     4621 tgtactcgtt tgttgccatt tctaagttag ctgcatagct agactcactt gagtaagcaa
     4681 tggtatcttc accagagact atccatttga gcaattctgc cttgatttct tcttgcactt
     4741 ctgcaggaat ttcgtcaaat gaggcaactg acttgtccaa gacaacccag cggtcaaggt
     4801 ctgtacgagc agatgtaatg gccataaatt cttggctatc cttaccaccc atggctccac
     4861 cgtcaccaat aatagccttg aagtctaaac cactacgagt gaaaatacgc tcataggctg
     4921 ctttgtactc atcataaaca ctatccaaac tatcatagtt agcgtggaaa ctataagcat
     4981 ccttcatgat aaactcacgt gtacgaagaa gtccattacg tgggcgtttt tcatcacgat
     5041 acttgggctg aatttgataa aggttgagtg gcaattgctt gtaagattta acagaatcac
     5101 ggacaatagc tgtaaaggtt tcttcgtgag ttggacctaa gataaagtct gatttttcac
     5161 ggttttttag tttgtaaagg tcttcaccat aggtttcgta acgacctgat tcacgccaca
     5221 attctgcact aagaagggct ggagccaaca tctcaacagc accaatcttt tcgaattctt
     5281 ggcgcatgat gtttttagct ttttcaatca cacgattggc aagtggtaga taagaataaa
     5341 cacctgctga aacttggcga acataaccag cacgcaacat aagagcatgg ctgataactt
     5401 gagcatcgct tggcatttcg cgaagcgttg ggataggcat tttactttgt ttcataatat
     5461 tcctcgatta tctaaaaaag agtcgcataa tgtcattcca agtcacagca atcatcaaga
     5521 caaccatgat gaccactccg gccaaggtga cataggtttc aatttcttgt ttcaatggtt
     5581 tgcggcggat ggcttctagg atattgagca caatcttacc accatccaag gctggaatcg
     5641 gaataagatt aaaaatccca atattgatgg aaatcattgc caagaagtac aagatatttt
     5701 caattccatt tttagcagca tcactacttg ccttaaagat agcaacaggt ccacccaact
     5761 tgttcaaatc cggttggaaa atcagatttt tcagagctga gagaattcgg agagctgagt
     5821 cagcagcagt tgtaaaacca cctacaaaca tggatagaaa atctgactta acccccggtt
     5881 gaacacctag aaggtaacga ccttgactat cttcgggtgt aacagtgact tgtttgtcac
     5941 tccccttttc agaaatagtc acatccaaag tcggtgccgt cttatctttg gtttctgttt
     6001 ccacagcttg gatcaagctt tcccagttgc taacctcatg tgagccaatc ttggtaattt
     6061 gtgccgtttc tggtactcct accttggcca aggcaccttg gggcatgata tggaactgat
     6121 tggtatcaac atctctgaca ccaccctgca taaagattaa aacccaaaaa acaacgacac
     6181 ctaagataaa attgttcata ggacctgcaa aattggtaat cagtttgccc cagatagtcg
     6241 cattttgata ttgaacatct aaaggtgcaa tccgaacctc agtaccatct gcttccacaa
     6301 ccgttgcatc gtgatccact gcaaatgttt tttcttcttc cagaaccaat cctttgataa
     6361 agagcttgtc ttcaaaatca aactgggtca cctgcatagg gagggctgtt tgatccaatt
     6421 ttttacctga gagattgatg cgtttaacct taccatcatc agcaagtgtc aaactaacag
     6481 gcgttcctgt cttgatttca gttgtatcat caccccaacc ggccatgcgg acatagccac
     6541 ccagaggcaa gattcgaatg gtataggccg ttccatcctt gccaatgtga gcaaaaattt
     6601 taggtcccat accgatggca aattcacgta ctaaaatccc tgatttcttg gcaaagtaga
     6661 agtgcccgaa ctcgtgcacc actacaataa tcccgaaaac cagaataaag gttaaaattc
     6721 cgagcatagc gtttcctccg tcttttgatt aaaagagtcc aaataagtgc atgattggaa
     6781 atacaagcaa catactatcg aaacgatcca aaacaccacc atgtccaggg ataaatttcc
     6841 cagaatcctt aacaccaaaa tgacgtttga tcgaactttc tagtaaatca ccaaattgtc
     6901 cagcaatgct aaagaaaata gcaaagactg acatcttgta aattccatat ggaagagcaa
     6961 ctgtactgtc aactatcata aagataatgg ttactaaaat tgctcctaaa ataccaccca
     7021 aggcaccctc aagggtttta ttaggcgata cccttggtgc taactttcgt ttcccatagt
     7081 tcatcccaac aagataggca ccactgtctg tcgcccagac gatacacaag gctaagagag
     7141 ccttgtccaa acctgcaaca cgagcatcta gtaaagcatt aaatccaaag cccacgtaga
     7201 agctcatagc aagagggaaa accgcatcct caatcgtata agacttgcta aaaacggtcg
     7261 ttcctaacat gattgaaatc aaaacactat aggcaaccac attcccatca actggcaaaa
     7321 aagtcaggta attctccaag ggaatggtca atgcaaaggt tgcaaagagg gtcaagaggc
     7381 cctccatcgt catggtctct agacctctca tcttcaaaag ttcatgcatg gctagcatgg
     7441 ctatgattcc gattgctatc tgaagcaaga ggcccccaat cattaaaatt ggtaggaaaa
     7501 tagccagggc aatccctgca aacaaggttc ttttctgtaa atcctgggtc atatttcctc
     7561 ctaaactcct ccaaatcggc gatgacgacg attataggca agaatagctt cctgcaaggc
     7621 cgcttcgtca aaatcaggcc ataaggtgtc cgtaaaataa agctcactat aggctccctg
     7681 ccatggaaga aaattgctca aacgtaattc tccactagta cggataatca agtctgggtc
     7741 tcgtaagtcc ttaggcaaat gctgagtaaa gagatagtta ccaatcaatt cctctgtgat
     7801 gtcacctggg ttgattttgg catctaaaac atcctgggaa atcaacttaa gcgcctgtgt
     7861 aatctcagca cgtccaccat agttaagagc aaaattaaga atcaatcctg tgttgttctt
     7921 agtcaattcc tcagccttgg ttaaagcttc aaaggtttgc ttaggcaggc ggtctgtctc
     7981 cccaatcatt tgaatcttaa cattattcgc atgtagttcc gggacataat tatcataaaa
     8041 ctctactggc aagttcatga taaacttgac ttcctgatct ggacgggtcc agttttccgt
     8101 agaaaaagca tagaccgtaa taaccttgac gcccagtttg ttggctgcct tggtcacggt
     8161 ttgcaatgct tccatgcccg ccttatgtcc aaaaactcgc ggttgcatac gttttttagc
     8221 ccaacggcca ttgccatcca tgatgatgcc gatatgagca ggaacctgtg tcggaacctc
     8281 tacttccaca gccttatctt tcttaaaaaa tccaaacatg atcttattcc tattcaaaaa
     8341 tctatcgttt cattatacca tatttcccca ttttcttcta tcactaagct atttattctc
     8401 aggcaccatg cccatttttc aaaaaaataa gccgcctgat tgggcgactt tatttttata
     8461 gggagattat tatgaaaaag ttttaggagt ttaagttaag gtcttcttaa cttatgaact
     8521 tagtgtacac tccctagctt aaagtttcct taagtatttt taaaaatcaa atttttccat
     8581 ttctcctgcc aatttttctt ggataaacgt gtttgataga gttccattcg gtcttcattt
     8641 tctaagaaat gaggagttgg acgaacttga aaattcaaaa tatcctccaa accataaggt
     8701 gcatagagtt caaaatctga ttcttcattc aagcgcagtc caactgccgt acaccgttct
     8761 ggatacttac tcatagcatc acgagaactg gtataggaag cagtgtgagg actgtgctga
     8821 tgcatataga cctgattttt caattcccac tggtactgag gaaaatcctc tctcagcttt
     8881 ttctccagta ataaggtttc ctcataagaa aaatctggat caaagaaaat cacatctata
     8941 tctgtttcat gatcaaaagg ggatttgtct gacaaaagat tccaaatgaa atttctgaca
     9001 gaacctgctg ccaaccacga gtctttcaga ccaaggtctc ggatgatcgt cagaatggcc
     9061 atcatatctg gattttctct aaaagcctct aagatttctt gcttattttt cactgtattc
     9121 ataacctaag tgctcatatg ccttagcagt cgccacccgt ccagaccgtg tccgcatgat
     9181 aaaacctttt tgaatcaagt aaggctcata catatcttca actgtctcac gctcttcggc
     9241 gatattcaca gaaagagttc ctagaccaac aggtcctcca ctgtacatct caatcatggt
     9301 acgaaggatt ttttgatcca catagtccaa accttcatgg tcaacatcca gcatagtcaa
     9361 agccttatcg gtaataatat catcaattac cccattcccc attatctggg caaaatcgcg
     9421 cacgcgcttg aggagacgat tggcaatacg aggggtccca cgactacgta gggccaactc
     9481 agatgctgcc tcatgagtga tttccatctc aaaaatatct gccgtccgct cgacaatttc
     9541 tgtcaagtca gcatgggcat aatactccat atggcctgta atcccaaaac gtgcccgtag
     9601 cggattggag agcataccag cccgagtcgt cgcaccaatc aaggtaaaag gtggtaactc
     9661 caaatgaaca ctacgactac cctcaccagc cccaatcata atatctatgt agaagtcctc
     9721 catagcacta taaagcacct cttccactga cattggcaaa cgatggatct catcaataaa
     9781 aagtacatcc ccaggctcta actcattcaa aatagctacc agatctccgg ctttttcaat
     9841 gactggaccc gacgtctgct taagattgac tcccagttcg ttggcaataa caaaggccat
     9901 ggtcgttttc ccgagacctg gaggcccaaa taagagcaca tgatccagcg cttcatcccg
     9961 cattttggca gcttcaataa agatttgtag ctggtccttg accttatcct gtccgatata
    10021 ttcacgtaaa tactgaggac ggagcgtgcg ttctactaac tcctcatccc ccattatctc
    10081 attatctaaa attctactca tggctctatt atatcaaaaa accaagccac aaacaaaaaa
    10141 gccacctgat tgggtgactc ctaagtttag cacttatgtg gtataatatt ctacgacact
    10201 gctacaccgc ctacgaaagg aggtgagata gcccatgatg gaattagtac tcaaaactat
    10261 catcggacca attgtggtcg gtgtcgttct tcgtatagtc gataaatggc taaacaagga
    10321 caaatagtgt caaaaaagac ctcaagctta tttggtcgtg agcttggggt cttttctagc
    10381 ctatgatata gaactagtac tcaattcctt tttattatcc catagttcac gaattttgtc
    10441 aaaggtttac attttcttca accgctgtac gacaagacgg ttaagattaa gagaacgtta
    10501 gggattctat caatttcata gaaattttga tttcgtaaac gaagagacaa tcttacatgt
    10561 cacttctcat ttaatacgcc actactagac aagcaaaatc attattacag tagttccagt
    10621 tcttcaatta acagtcactt acaatcaaat tgagtttgaa ctagctgaag cgaccacaga
    10681 cctatttctt agttatattc gctaaaaaag tccccgccaa ttccccgacc aaaatccgaa
    10741 aaataccgaa aaatatcgaa aaattatttt tagaatagtc cccaaaagcc tgaaatagag
    10801 ctaaaaaact ccacctgatt gggtggagtt aagggagatt attatgaaaa agaaaagttt
    10861 aggattttat taaataaagt taggaggtct ttatttaatg actatataat actagaccat
    10921 ccttaaattt tgcttaagaa aaaacaagtt ttgttatttt ttacgattca tccaagtcat
    10981 ccctatacaa ttataggtgg ataagatttc aaagcccata gaacgataga agcccaaggt
    11041 tctttctgtc tcttctgtca ccagctggac ttgataggca tctttgtaat cctctaaagc
    11101 ctctttcatc aaggcactac caattccttg acgctgatag attggtaaaa cgattaaatc
    11161 ctgaaccaat actgatgaga atccatctcc aaccaaacga atcaagccca ccacagcatc
    11221 accatcaagt gccagataaa ttactaatga atgagataag gcctgctcca gcatctctgg
    11281 ttgatgggta taatttgtcc aaccgacagc ctgatagaga tgtaaaacat cctctagctt
    11341 gacaatttct tgctttttaa tagtaatcat ctcaacacct cttaaagttc tctcaagctc
    11401 ttgtactgct gtccattttt atcaaaattt tcaggacgca accatgtttc caaacgatct
    11461 ttgacttgag gccagtcctt atcaatcata gacaaccaat ccgtatctct cgtacgtccc
    11521 ttataaacca ctgcctgacg gaaggttcct tcataaataa agctcaaacg ctccgcagct
    11581 cgtctggatg gcagatttaa ggcatcgcat ttccactcgt agcgacgata gtttagctcc
    11641 tcaaaaacat agcgagctag gagatactga gcttcagttc ctatccgtgt accccttagc
    11701 tctggagaaa aagtgactgc tccgacttct attactcggt tattctggtc aatgcgcatg
    11761 agagaaaaag atcccaaagc cttaccagtc gccttgtcta tgattgcata gtaaaaacgg
    11821 tccttacgag ccaacatctg atgtaaaagg ctaaccagct cccccatatc tgctactggc
    11881 tcctgaaaaa ggtaggtcca catctcctga ggcgtatcag gcccataaac agctagcaaa
    11941 tcctccgcat gcttttccac tgaaagagcc tctatcctag cataacgccc ttctaagaaa
    12001 tcaatagaag gcagttcacc tggtgtataa ccttccattg actccccaat catttgacca
    12061 tattcattta ctggcatagc tttctccttt tttcactttt caaactttat agttgcccat
    12121 agtataccat actttcataa gaagattcaa aaaatcctcc agattctact ctgaaggatt
    12181 cctaccttat ttcacaacga tattaacgag tttagttggc tcaaacagtt tgggaaactg
    12241 tttgaggttg gaaatcagca aataaattgg ttattttaca acaatattaa ccaatttatt
    12301 tggtacacta atcactttca cgatttcctt accgtcaatt tctgctttga ctttttcatc
    12361 cgctagagca atttcttgca attcttcacg tgataggtct ttagcgacca tgagtttggc
    12421 acggacttta cctttgattt ggacaacgat ttcgatttcg tcttcaacca gtttactttc
    12481 gtcccatgtt ggccaagtca cgtaagagat tgactcacct gttgcagcaa cagtttgcca
    12541 gagttcttct gccaagtgag gtgcaaatgg ggcaatcaat tggataaatc ctttggcata
    12601 gtcaacatag agcttgtctt ccttgttagc cgcattgaca aagaccataa gttgggcaat
    12661 ggctgtgttg aatttgaggg aatcaatttg ctctgtgaca gctttaacag tttcgttgta
    12721 aaccttgtca agagcgccat tgttttccgc aacaatttcc ttacttctaa tcaaacggta
    12781 aacacggtca aggaacttac gacttccttc cagaccttct tctgaccaag caatcgaagc
    12841 atcgagtggt cccatgaaca tttcatagac acgaagggta tcagcaccgt attgttccac
    12901 cacatcgtct gggttgacaa cgttcttgag cgatttagac atcttggctg gcgcttgctc
    12961 caactcttcc cctgtttcca catggaagaa agaaccatca cgtttttcaa ccttgtcagt
    13021 cgccacaaga gccccacggt ggtcacgata gcttgttccc aaaatcattc cttggttaaa
    13081 gagtttttgg aatggttcct tagttggaac aacaccgagg tcatagagga atttatgcca
    13141 gaaacgagcg taaagcaagt gaagtacagc atgttccgca ccacccacgt agatatctac
    13201 tggcaaccat tgtttgagca gatcctcgtc agccaatttc tcagtattgt gtgggtcaat
    13261 atagcggagg tagtaccagc ttgaacctgc ccattgtggc atagtatttg tttcacgacg
    13321 acctttgaca ccatcttcac gagtcacttc aagccagtct gtcaagttag ctagtggact
    13381 ttcaccagta cctgagggac ggatatcctt ggttacaggc aaaacaagtg gcaattcact
    13441 ttcagggaca gctgttgaag ttccatcttc ccaatgaatg attgggattg gctcacccca
    13501 gtaacgttga cggctaaaga gccagtcgcg gagacggtag gttaccttct cttgtccaca
    13561 gcctttttct tccaaccaag ccacaatctt ggcaatagcg tcttctttgt tgagtccatc
    13621 taggaagtct gaattaacgt gaaggccatc ttctgtgtag gcagcttctt caacatttcc
    13681 accttcaagt acttcgacga ttggaaggtc aaattgtttg gcaaattccc agtcacgttg
    13741 atcgtgggca ggcacagcca ttaccgcacc tgtaccatag ctagcaagaa cgtaatccgc
    13801 aatccagatt ggaatttcct taccattgac aggattgatg gcataagcac cagtccatac
    13861 accagttttt tctttggcaa ggtcggtacg agccaagtct gacttaaggc tggcttggtg
    13921 tttgtagtct gctactgctt ctgcttgctc tgtgcttgtg atggcatcta ctaattcatg
    13981 ttcaggagcc aaaacagtga aagtcgcacc gaaaagtgtg tccggacgag tagtaaagac
    14041 tgtaaattcc ttgtctgttc cttttacttt gaaagttaca ttggcaccag ttgatttacc
    14101 aatccagttg cgttgcatat ccttgataga ctctgaccaa tctagttcat ctaagtcatt
    14161 gagcaagcgc tctgcgtaag ccgtgatttt gagcatccat tggcgcattg gtttgcggac
    14221 aactggatag cctccacgtt cagaagttcc gtcaggaagc acttcttcat tggcaatggc
    14281 agttcccaat tcctcaaccc agtttactgg cacttcagct tcataggcca agcctttttc
    14341 gtaaagcttg gtgaaaatcc attgagtcca cttgtagtag tttggatctg ttgtgttgac
    14401 ttcacgatcc cagtcataag aaaatccaag cgcattgatt tgacgtttga agttggcaat
    14461 gttttccgct gtaaattctg ctgggtcatt accagtatcc atagcgtatt gctctgcagg
    14521 caaaccaaaa gcatcccaac ccattgggtg aaggacattg tagccttgcg cacgtttgta
    14581 acgactgagg atatcggttg cagtataacc ttctgggtgt cctacgtgca gaccagctcc
    14641 agacggataa gggaacatat caagcgcata aaacttaggt tttgatgcat ctgttcctgt
    14701 cttaaatgta tgatgttctg cccagtagcc ctgccactta ggctcaattt ctttatgatt
    14761 gtaaaaactc atggtctctc tccaattttg tgatgttact attataccat ttttgaggga
    14821 atttgaaatg tgtttaaagg aaaaactgta tactgtatct cattaatttt gatgactatg
    14881 aattgctcta tctcccactt tttatcccat tgttatgcag taaatcaagg agtaagtcaa
    14941 gaaacttatc tcgtatagca caaaatactt attttagacc gaagataaaa ctggaatgct
    15001 aattaatgta ttcacaataa atagccgaca acctatgtcg ctttttgatt tatgaagaac
    15061 gtatagtgga ctgaatctga aatagtacga aacaattgct aaaata
//
LOCUS       DAVBHS010000037        14810 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVBHS010000037 DAVBHS010000000
VERSION     DAVBHS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14810
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.037"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            150..488
                     /locus_tag="VQ651_000833"
     CDS             150..488
                     /locus_tag="VQ651_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET2753854.1"
                     /translation="MTLTIHVYYKGDGDCAIKFAREMLESGLVAEIRCQKGNLKYEYF
                     LPIEKEGIILLIDQWINQEALDEHHQSNTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            678..2114
                     /locus_tag="VQ651_000834"
     CDS             678..2114
                     /locus_tag="VQ651_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET2753855.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPVTCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTDQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKKVILSNGESVE"
     gene            2166..2405
                     /locus_tag="VQ651_000835"
     CDS             2166..2405
                     /locus_tag="VQ651_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HET2753856.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            2535..2849
                     /locus_tag="VQ651_000836"
     CDS             2535..2849
                     /locus_tag="VQ651_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HET2753857.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            2967..4946
                     /locus_tag="VQ651_000837"
     CDS             2967..4946
                     /locus_tag="VQ651_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET2753858.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFAQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNSFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSYVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFISIFEKIK"
     gene            4956..5270
                     /locus_tag="VQ651_000838"
     CDS             4956..5270
                     /locus_tag="VQ651_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HET2753859.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            5301..5807
                     /locus_tag="VQ651_000839"
     CDS             5301..5807
                     /locus_tag="VQ651_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753860.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFINPSPIIIFPIMLIVFSILATVEQFKLYGNRKEKLGITLMFVRIQILISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            5887..7233
                     /gene="celB"
                     /locus_tag="VQ651_000840"
     CDS             5887..7233
                     /gene="celB"
                     /locus_tag="VQ651_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HET2753861.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            7613..7789
                     /locus_tag="VQ651_000841"
     CDS             7613..7789
                     /locus_tag="VQ651_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753862.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            8199..10412
                     /locus_tag="VQ651_000842"
     CDS             8199..10412
                     /locus_tag="VQ651_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HET2753863.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHFFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(10634..11110)
                     /locus_tag="VQ651_000843"
     CDS             complement(10634..11110)
                     /locus_tag="VQ651_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET2753864.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            11333..14533
                     /locus_tag="VQ651_000844"
     CDS             11333..14533
                     /locus_tag="VQ651_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HET2753865.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSADASTRVIEAKDGA
                     ITISSHEKLRAAVHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESDKDKRLWNSA
                     TTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQPSEDYQTD
                     KQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKEV
                     KKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSIS
                     SQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSMEW
                     MHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDPE
                     HFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGFY
                     QDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAPL
                     LVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQS
                     DSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGFG
                     LSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTET
                     DAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFLG
                     SNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDSK
                     KNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYML
                     IPNVGRATFNQMIKELERSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVLK
                     RGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKNH
                     SDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
BASE COUNT         4933 a         2145 c         3043 g         4689 t
ORIGIN      
        1 atcgctataa gaaaaaatca ttttattggt ataaagaagt gattgaaagt aatggcgaaa
       61 cgttagataa ttagttcata aaaaacaagt ttagatcctc atctaaactt gtttttttaa
      121 aattgaagga tgattttgga ggatatgaga tgacattgac tattcatgta tattataaag
      181 gcgatggaga ttgtgcaata aagtttgcaa gagaaatgct tgaatctggg cttgtcgcag
      241 aaattagatg tcaaaaaggt aatttgaaat acgaatattt tttaccaatt gaaaaagagg
      301 gaattattct ccttattgat cagtggataa atcaagaagc tttagatgaa catcatcaat
      361 caaatacaat gcagaaaatc ttagatttaa gaaaaaaata tcacttgcag atgcaagttg
      421 aacgttatat agaagatgat tctgggatgc cagaaagcga taagtcgttt atagacacag
      481 ggaattagaa ttattgtgag agtttttgta gtttgaaagt attgaaaatc gtaaagatat
      541 cattacgaga atacaagaag atattgttct atttccatta aaaattactt ctttccttaa
      601 taataaggaa aaagaatacg ttttcatctt ttggtttgag gtttataata agaatatcaa
      661 agtcaggagg aaaaaagatg actactttta aagatggatt tttatggggt ggtgctgttg
      721 ctgctcatca acttgaaggc ggatggcaag aaggtggcaa aggaattagt gttgctgatg
      781 ttatgactgc tggtcgccat ggagtagctc gtgaaattac tttgggagtt ttagagggta
      841 aatattatcc aaatcatgag gcgatagatt tttatcatcg ttataaagaa gacatagcac
      901 tttttgctga aatgggattc aagtgcttcc gtacctctat tgcatggaca cgtatctttc
      961 caaaaggtga tgagttagag ccgaatgaag aaggattaca gttttatgat aatctttttg
     1021 atgaatgctt aaagaatggt attgaacctg tcatcactct atctcatttt gaaatgcctt
     1081 atcacttagt gaccgaatat ggtggttgga aaaataggaa attgattgat ttctttgctc
     1141 gttttgcaga agtcgtattt aaacgttaca aagataaggt taaatattgg atgactttca
     1201 atgaaatcaa taatcaagcg aattatcagg aagattttgc accatttact aactcaggta
     1261 ttgtatatga ggaaggtgat aatagagaag caattatgta tcaagcagca cattacgaat
     1321 tagttgcttc tgcacgagct gtaaaaattg gtcatgagat taatccagat tttcaaatag
     1381 gttgtatgat tgcgatgtgt ccaatttatc cagttacttg caatcctaag gatatcttaa
     1441 tggcaatgaa agctatgcag aagcgttatt attttgctga tgtgcatgtt ttaggtaaat
     1501 atcctgagca tattttcaag tattgggaac gaaaaggtat ttcagttgat tttactgacc
     1561 aggataaaga agatttactt ggtgggactg tagattacat tggtttcagt tactatatgt
     1621 cctttgctat agactctcat cgtgaaaata atccttattt tgattatctt gaaacagaag
     1681 atttagtgaa aaataattat gttaaggctt ctgaatggga gtggcaaatt gatccagaag
     1741 gtttacgtta tgcgttaaat tggtttacag accactatca cttaccactc tttattgttg
     1801 aaaatggttt tggagctata gatcaagttg cagcagatgg tatggtacat gatgattata
     1861 gaattgaata tctaggtgcc catattcgtg aaatgaaaaa ggctgtagtt gaagatggtg
     1921 ttgatttaat gggttatact ccatggggat gtattgattt ggtttcagct ggtaccggtg
     1981 aaatgcggaa acgttatggc tttatttatg tagataaaga tgacaatggg aagggaagtt
     2041 ataatcgttc cccgaaaaaa tcttttggct ggtataagaa agttatttta tctaacggtg
     2101 aatcagtaga atagtataaa caaactgaga ttattctcag tttgtttagc atattgagga
     2161 gacatatgat tggaagtatt gcagcaattt taacaacatt tgcattttta ccacaagttt
     2221 ttcgtgtagt taagacaaag gacacaggat ctattgctct aggtatgtat gttatgcaag
     2281 ttattggtat tgcattgtgg cttgctcatg gtattcgtat aggagacttg cctttgattt
     2341 tagcaaatag tgtttcattt ctactctcag gaataatttt attttataaa ttgaagtata
     2401 aataattttt ttccatatca tttaggaaaa tgagggaatt gtcattgaaa acgctttatt
     2461 ttataataaa aatagataac aaataagcca acttagcaca gctttgattg gatttaagaa
     2521 aaggagaaaa tattatggca aaagctttaa ttatttgtgc agcaggaatg tcttcatcac
     2581 ttatggcaca gaaaacaaca gagtttctaa aaggaaaagg gaaagatatt gtagttgatg
     2641 ctattagtgc tactgaagga gggaaagcta tcgcagcagc agcttttgat ttgtatcttg
     2701 tcagtcctca aacaaaaatg tactttaagc aatttgaaga ggctggttct aaagcaggaa
     2761 aaccagttgt acaaatacct cctcaagcct atattcctat tccaatggga attgaaaaaa
     2821 tggctaattt gattcttgag aatatttgat gaacacatga aatgtcaata aatatttaag
     2881 ttaagataaa ttgttttgag tttgatataa attgatttaa gtgtataata tgcttcaata
     2941 tatgaaaaca tacgttttag gagggaatga atgtgttaaa cattaaagag aaagatatcc
     3001 ttcaattttt aataaaaaat aaggaacgtt ttgttacaag caaagaactg gcagaatatt
     3061 tatcctgttc agatagaact gtccgtaatg tactgaaatt aattgagaag acaatgatta
     3121 ttcaaggagt ccggttgatt tcaaaacaag gtcaagggta tcaaatattt tttgagaatc
     3181 aaggcgctta ccaggagttt agacaaactt atgaactgga agaagactat acgaaaactg
     3241 cggtttcaaa aggtgatgat cgtttagtat ttatcttaaa taaattatta tttgagcaag
     3301 ttccagttct ttttgatgat ttagctgatg aactttacgt tagtcgttca acattatctc
     3361 atgatttcag aaagatacga gtgatgttat cagagtataa tttgtccatt gagagtaggg
     3421 cgaataaagg agtttatgtc tcaggcgagg aacgtgataa acgacgtttt atcataaatt
     3481 atttcctaga gaatcaattt tttaaaacaa tacattgcta tgtaaaattt aacttttttg
     3541 ctcaaactgt tccattagaa gaatttgcta gaattgtttt ggatgaatgc caagaggcca
     3601 acctaaaact atctgatttt gtactacaga atttagtagt tcacatagca ttgtctgtga
     3661 ttcgtttaaa atccggattt gaaataaaaa atatagactg tcagatgact gatgatgcaa
     3721 tagagagaaa ggttgctcaa agaattcttt ctaaagtaag agaagtaaca aatcaggaat
     3781 ttccagttca ggaaatagac tacattactt tgcatttgtt agctaagagt caacaatgtc
     3841 aaaaaaatca gaagaatatt tctgaagaag ttttaaaaaa atctttattt aaaacatttc
     3901 agaatttagg attagatgat atgtataact tttcttcaga tttccaacta attgaaggat
     3961 tgataactca tctaatgaca ctacaagtac gtttagaaag ccggattacc ttaaataacc
     4021 ctttggtaga tgaaattaaa cagaattata gtgatatatt ctttatgact agagaaattc
     4081 ttgcaaatat ggacaagttc ttggagtgga caatatcaga tgatgaagta gcatatgtaa
     4141 gtcttcactt tttagctgct atggaacgta gcaaagagag tactaagttt aatattcttg
     4201 ctatttgtgc aacaggattt ggtgcagccc aaatgttaag aaatcgtttg gaaacggagt
     4261 ttgggaagcg agtagaagtt gttgatgtaa taggctatta tgaattgaat caagaaaaac
     4321 ttaaaggaat agattttata gtgtcagctg ttgatttatc aaatctatat tttcaaattc
     4381 cagtttttaa agttagtgtg tttcttaaga gtgatgagat ggaaatgatt agaaaagcta
     4441 tggatcagat gcaagtttct agttatgtac aatcatcaaa gattaataaa tttgaaaaca
     4501 acagctttag acagtatttt agtaaagaaa attttttaat atgtactgaa tcagacaagg
     4561 taaatttact tgaaaagatg gttgagagtt tatctgttgg tgaatcaaat gagtttgaac
     4621 aatctttact ttatggaatt aaacagcgcg aagaattaag ttcagttgtt ttttctgaaa
     4681 aaattgcagt tccgcatcct attcagccct ttggtacaga gggaaaggtt tcggttgcaa
     4741 tctgtaaaga ttcgttactc tgggataatc aaagttcata tgtccagcta gtatttcttc
     4801 tatctccatc aatatatggg aacgaaggtt tggcaactgt gactaaaaag atagtctctc
     4861 tgacagagaa tgatgagttg caaaatcagc taatatcttg caataacttt gaagatttta
     4921 ttagcatatt tgagaagata aaataggagg ttcatatgaa tcaagaggaa ctgcaggttg
     4981 cagcatttga aattatcctg cattcgggaa atgcaaggtc agaaatacat gaagcatttg
     5041 caaaaatgag agaaggtagt tttgatgatg cagaaagtaa attaaatcaa tcaaatgaga
     5101 taattcttga agcgcatcat gcacaaacca aactcttgca agaatacgca agtggagtgg
     5161 aaattaaaat tgaaattatc atggtacatg ctcaagatca tttaatgacc actatgacat
     5221 tattagaagt agcaaaggaa atgttagctc tttataagaa agttaactaa atatgtgttg
     5281 ttatataagg aggaaatgat atggataaaa aagaacgaca aaagattgaa caacaacgtc
     5341 gagagatggc tctaactaat acttttttta atcgatatct tcttctacgt tattcaatag
     5401 cactcttctt ttttgggaat atttattggc ttttgaatca gtttattaat ccctcgccta
     5461 ttattatttt ccccattatg ctcatagtat tttctatttt agcaactgtt gaacaattta
     5521 aactctatgg aaataggaaa gaaaagttgg gaataacact aatgtttgtt agaatacaaa
     5581 tacttatttc aataggatta cttgttttaa cttggacaag ttggtttaag aatctttttc
     5641 cgatttttga aaataaccaa gtagcacgtt tatttgtttt tgtagttctt ctattaggtt
     5701 tagttctgag tttgttagac attagaagaa ttaaaaaaat ttataaacga acagataagg
     5761 cttatcaaca atttgtccaa ttggaaaaga actcacttag tttataatcc tgatctagta
     5821 taatttgtat ttgaaactag tctttagaaa gtaaaaatta ttatgtttta ttaaggaggt
     5881 ctatttatgg atcaacaaaa cgggttgttt ggttttcttg aaaaccatgt tatgggacca
     5941 atgggcaaac ttgctcagtt taaagtagtg cgtgctatca cggctgcagg tatggctgct
     6001 gtaccattta ctattgtagg atcaatgttt ttggtattca gtattttgcc acaagctttc
     6061 tcattttggc caattgtggc agatattttc tctgcttcat ttgataaatt cacatcactt
     6121 tacatggttg caaactatgc gactatgggt tctctatctc tttatttcgt tctatcactt
     6181 gcatatgaat tgacaaaaat ttatgcagag gaagaagaac tcaatatgaa tcctcttaat
     6241 ggtgccttgc tcgccttgat ggcttttgtc atgacagtac cgcaaatcat ttttgatggt
     6301 ggaatgatga agactgtgac aagtctaaaa gaaggtgcag taattgcaga tggatgggca
     6361 atgggaaatg gagtcgcacg ttttgggaca acagggattt ttaccgcaat cattatggca
     6421 attgtgactg ttcttattta tcgtatgtgt gttaaacata attgggttat taaaatgcct
     6481 gaagctgttc cagaaggagt ttctcgtgga tttaccgctt tggttccggg atttgttgtt
     6541 gcatttgttg ttatctttat caacggtctt cttgtagcaa tgggaacaga tatttttaaa
     6601 gtcattgcaa ttccatttgg ttttgtatcc aatctgacta attcgtggat tggtttaatg
     6661 attatttatc tattgactca actactttgg attgtaggta tccacggtgc gaacattgtt
     6721 tttgcatttg ttagtccaat tgctcttgct aacatggctg aaaatgctgc tggcgggcac
     6781 ttcgctgttg caggtgaatt ttctaatatg tttgtaattg caggtggttc tggtgcaact
     6841 ttaggactat gtttatatat tgcttttgcc tctaaatctg aacagcttaa agcaatagga
     6901 cgagcatctg tagttccagc cttatttaat attaatgaac cattaatttt tggattacct
     6961 attatctata atccagcctt ggctatacca tttattttag caccaatggt tactgctact
     7021 atttattacg tagcgaattc tctaaacttt attaagccaa ttatcgcaca ggttccatgg
     7081 ccaactccag tagggattgg agctttctta gggacagcag atcttcgagc tgtattagtt
     7141 gctctagtat gtgcatttgc agcattctta gtctatcttc cattcatccg tgtatatgat
     7201 caaaaattgg tgaaagaaga gcaaggtatc taataagtaa aaaagtatac tcgatgatag
     7261 aatccatata gtttaaataa tgagtgttaa tattagcagt gaagaggtct ttttaagata
     7321 ttcttctctg ctttttcata ttttttcgct atgtagggta tgaagcaaaa tagaaagtag
     7381 ttcgaatagt tgataaacag gaaactgctg ttatttaaaa aaaacaataa agtaaagtat
     7441 gttatcgtag aatacgatac aatagtaaaa aagcaaaaag aatgcgtttt atatttgggt
     7501 ttgaggttta taaaatagga taagcgtgct gaaaacttag atatgacaaa caaaataaaa
     7561 tttttaaaag ctacatagaa acatggtata atgtatctaa aggaggtagc atatgtcctt
     7621 agatatagat aaagaaaaga tgacaatcat gggaatagct tttgaaaaca gatccgtttt
     7681 taagagtgtt tggtatgctt taagtacaaa tatgattgaa ggatggcgac ctacggttag
     7741 tgatgttgaa aaattacgag atgaagctct tgctctgggg atgacttaat gaagcgcaac
     7801 tgttatgagt cttatgacta tgttgatccg gataatttat ataccgatcc gggttcttca
     7861 gttctaagaa ataagcagga aggaaaaaag cacgcgagct tgaatatcgt atgattgcta
     7921 gtaagagttt gaaattattt atcaatccta tcttagttaa tagtttaaaa gtagtttgtt
     7981 tcacaactgc ataagcttag aaaagttgaa aagtgataac tctttaataa actcttggtt
     8041 tgttaaaaga tattttgtcc actacatttt ttcaattgac tattatttgt catagatata
     8101 acagatagaa cagatagaac attgcattca gaataatgaa aaacgcatca aaaatggtgc
     8161 gttctgtttt tatgctacca taaaaggagg aaaacaaatt gaaaattttt aagggagagt
     8221 tttatcgaat ctctgtatta acagacaagc tagtaaggtt agaatactct caaactggaa
     8281 gttttgagga tagaacgaca caacttatct ataatagaga ttttggccaa gtttcgttag
     8341 attatatcga gacatcaaac gtactagata ttatgacgga ctattttcat ctgcacttta
     8401 ataaaggaga atttaacgcc gaaaatttat ttatagaatt aaaaggaaat tttgccgtat
     8461 atggtagtcg ctggtatttt ggtgaatcta ttgaaacgtt aaaaggaaca gctcggactc
     8521 tggataaggc agatggagca atctcgttag aagatggaat tattagccga aatggtatag
     8581 ccttattgga tgattctcaa ggatttattt gggatgaaca atctggttat attgagagag
     8641 aaaatcaaat tgacctgtat ttctttgcct atgggcatga ttatagagga gcaatcagag
     8701 acttttacca tttgactggt tcaacaccct tgttgccaag atatgcttta ggcaattggt
     8761 ggagtagata ttggccttat acgtcggatg aatacttgga tttaatagac agatttgaaa
     8821 cagagaaaat tccattatct atcggtgtgt tagatatgga ttggcatata actgacattc
     8881 cagctcgttt tggaagtggc tggacaggat atagttggaa tagaaactta ataccaaatc
     8941 cagaacagtt attgcaacaa cttcatgata gaaagttaaa actctcctta aatgtccatc
     9001 ctgctgatgg gatacgggct tatgaagaag cttatcctca aatcgcaaaa cggttggggt
     9061 taaatgtaga actagaagaa cctgctattt ttgatttttt taatccctct tttagggaag
     9121 cctactttaa agatgttcat tatgagctag aaaagcaggg agtagatttt tggtggattg
     9181 actggcaaca agggacacaa ggtatgctag atccactttg gcttttaaac cattatcact
     9241 atcaggatag ttgtaaaaat gcagaaggtg gtttgatttt atcaagatat gcaggtcctg
     9301 gtagtcaccg ctaccctgtt ggtttttcag gggatactat tattagttgg aattccttaa
     9361 ggtttcaacc ctattttaca gcgacagcat ctaatatcgg ttatagttgg tggagtcatg
     9421 atatcggtgg acatatgttg ggggattatg acgaagagct acaaactaga tggctacagt
     9481 ttggtgtttt tagtccgatt actcgattac atagttctag aagtcctttt aatagtaaag
     9541 aaccttggtt cttttcagaa acaacatcta agattatgaa gaaatacctt cgtttgagac
     9601 atcagatgat tccctatcta tataccatga atgtaaagac acatgaggaa ggtgccccat
     9661 taatcagtcc aatctattat ttctatccag agaatgatga gagctataat gttccaaacc
     9721 aatacttttt tggaacagaa ctgatggtgg ctcccattgt agaaaagatg gatttgacat
     9781 tccaatctgc aaaagtagat gtatggttcc ctgaaggtga atggtatgac ttcttttcag
     9841 agaaaaaata cacaggtggt gtgaagttaa gtgtttatag ggacatctcg actacgcctg
     9901 tgtttgcaaa aagtggtgca atcattccct tggttggttc tgagataggt atgggtgttg
     9961 atttacctga ggttgtagat tggtatgtat tcccagggaa acaacatttt tttgaaatgc
    10021 ttgaagatca aaatggtcaa agatataaaa caagattatc aatcgattgg gaaatgggaa
    10081 tggtagagtt agcattacaa ggagattcta gtatcgttcc aagcaataga aaacatagaa
    10141 ttcattttaa aggaacgaat gtgtctataa ttgaattgcc aaataagaat gatacagcta
    10201 aatttgaatg gaaagataat aaaaggacat ctttaaatga cgaagttttt agactactaa
    10261 agacagcttc tcttccatat gaattaaaag atagattgtt aaatcaattc atcaatgcga
    10321 aaaactctca tgacttaatg aatatcttgc atcatcagga taaggaattg agagggcgtt
    10381 tgttggaaat gatatttact agccaaaact aattgaaata attagaaagt catttgttta
    10441 gtgtatatga agattctgta tggtattcgt tatcactggt cacattgaaa atgtgaaata
    10501 agcacctaca acatagactt tacaacactt catgtttcat atcatggggt gtttttctat
    10561 ttaagtcaaa gcccatcaaa aaatctcact attcatcagg ttaaaggaaa cctaatcata
    10621 gtgagattgt gaattatagt agagtttgta tcgcctcttc aatttgtttt gggtctgttt
    10681 ttgaagaaaa gcgttcaaag acttgcccat ctcgaccgat gagaaactta gcgaaattcc
    10741 attcgattcg ttttcctagt gggccggatt tctggtcttt taaccagaca tagagagggt
    10801 ctgcttcctt accgttgacc ttaatcttgg caaaacgtgg gaaggtggtt tgaaaatgta
    10861 ggctacagaa ggcgttgatt tcctctgcgc tgccgggtgc ttgtcccata aactgattgc
    10921 aagggaaatc caatatttcg aagccctgat cttgatagcg ttcatagagt tcttgaagtc
    10981 cctggtactg gggcgttaaa ccacatccag tagcagtgtt gacaatcaag agaactttgc
    11041 cacgatagct atccaaggga gttgcttggt tgttttggtt caaaacggaa aaatcataaa
    11101 gtgaagtcat gagggcctct tttctttttc tacattgtaa cagtttttac ttttttcgtc
    11161 gagtcggatg gaagatattg ttgaagaata agccttcaat atgatagaaa ccgtttacaa
    11221 ataactttaa aaatggtatg atttaattaa accggtttac ttagttgcta taaatctcta
    11281 atttgagatt tagttcctat tgattttaca atatgtttat tggagtgtat acatgcaaac
    11341 aaaaacaaag aagctcattg tgagtttgtc ttcacttgtt ttatcaggat ttttattaaa
    11401 ccattatatg acagttggag cggaagaaac gactacgaat accattcagc aaagccagaa
    11461 ggaagttcag tatcagcaaa gggatacaaa aaatttagtt gaaaatggtg attttggtca
    11521 gacggaggac ggaagcagtc cgtggacagg aagcaaagct caggggtggt cagcttgggt
    11581 agaccagaag aatagtgcag atgcctcaac tcgagtcatt gaggctaagg atggggctat
    11641 cactatctca agccatgaga aattaagggc agcggttcac cgtatggttc ctattgaagc
    11701 taagaaaaag tataaactgc gtttcaagat taaaacagat cataaagtcg ggattgccaa
    11761 agttcgtatc attgaggaaa gtgataagga caagcgattg tggaattctg caacgacgtc
    11821 aggaacaaag gactggcaga ccattgaagc agactatagc ccgactttag atgttgataa
    11881 aatcaagctg gagttattct atgaaacagg aactgggact gtttccttta aggatattga
    11941 gctggtagag gtagcagacc agccttctga ggactatcaa acagataaac agcttgagga
    12001 aaagattgat ttaccaattg gaaaaaaaca tgttttttct cttgcggact atacttataa
    12061 ggtagaaaat cctgacgttg cttcagtcaa aaatggaatt ttagaacctc ttaaggaagg
    12121 gacaaccaat gtcattgtca gtaaagatgg caaggaagtg aaaaagattc ctttgaagat
    12181 tctagcctct gttaaggatg catacacaga ccgtttggat gactggaatg gcatcatcgc
    12241 tgggaatcaa tactatgatt ctaaaaatga acagatggcc aaattaaacc aggaattgga
    12301 aggaaaggta gctgatagcc tatctagtat ttcaagtcag gcggaccgca cctatttgtg
    12361 ggaaaaattt tcaaattata aaatgtctgc aaatctgact gccacttatc ggaaattgga
    12421 ggagatggcc aagcaagtga ccaatccttc ttctcgttat tatcaagatg aaactgtcgt
    12481 tcgaacagtc agggattcca tggaatggat gcataaacat gtctacaata gtgaaaagag
    12541 cattgttggg aactggtggg attatgaaat cggtacacct cgtgccatca acaatacctt
    12601 gtctctgatg aaagaatact tctctgatga ggaaattaaa aaatatacag atgtgattga
    12661 aaaatttgta ccagatcccg aacatttccg aaagacgact gataacccat ttaaggctct
    12721 aggtggaaac ttagttgata tgggaagagt aaaagtaata gctggtttac tgcgtaagga
    12781 tgatcaagaa atttcttcta ccattcgctc gattgagcaa gtgttcaagt tggtagacca
    12841 aggtgaaggt ttttatcaag atggatccta tatcgaccat accaatgttg cctatacggg
    12901 tgcttatggg aatgttttga ttgatggcct gtctcaactg ttgccagtca ttcaaaagac
    12961 caagaatcca atcgataaag ataaaatgca aaccatgtac cactggattg ataaatcgtt
    13021 tgctcctttg ctggtgaatg gagagctgat ggatatgagt cgtggacgct cgatcagtcg
    13081 tgcaaatagc gaggggcacg tggccgcagt agaagtacta agagggattc accgaatagc
    13141 ggatatgtct gaaggagaaa ccaaacaacg tttgcagagt cttgtgaaga ccattgttca
    13201 atcggatagt tattatgatg tctttaagaa tttgaagact tataaggata tcagtttgat
    13261 gcaatccttg ttaagtgatg caggagtcgc aagtgttcca agaacaagtt acctatctgc
    13321 ctttaacaag atggataaaa cagccatgta caatgcagag aaagggtttg gatttggctt
    13381 gtcactcttt tccagtcgta ccttgaatta cgaacacatg aacaaggaaa ataaacgtgg
    13441 ttggtatacg agtgatggga tgttctatct ttacaatggc gatttgagtc actatagcga
    13501 tggctactgg ccaacagtta atccatataa gatgcctggt acaacagaga cggatgctaa
    13561 gagagcggat agcgatacag gtaaagtttt accgtctgct ttcgttggaa cgagcaaact
    13621 agatgatgcc aatgcgacag caaccatgga tttcaccaac tggaatcaaa cattgactgc
    13681 tcataagagc tggtttatgc taaaggataa gatcgccttt ttaggaagca atatccaaaa
    13741 cacttcaaca gatactgctg caactacaat tgaccagaga aaactggaat caagtaatcc
    13801 atataaagtc tatgtcaatg ataaagaagc ctcccttaca gaacaagaaa aggattatcc
    13861 tgaaacccaa agtgtctttt tagaatcatc cgattcgaaa aagaatattg gttacttttt
    13921 ctttaagaag agttcaatca gtatgagtaa ggctttgcaa aagggagcct ggaaggatat
    13981 caatgaagga cagtcagaca aggaagttga aaatgaattt cttacgatta gtcaggctca
    14041 taagcaaaat ggagattctt atggctatat gctcatccct aacgtgggtc gtgccacctt
    14101 caatcaaatg ataaaagagt tagaaagaag cctcatcgaa aataacgaaa cccttcagtc
    14161 tgtttatgat gccaaacaag gagtttgggg cattgtgaaa tatgatgatt ctgtctctac
    14221 tatttccaac caattccaag ttttgaaacg tggagtctat accattcgaa aagaagggga
    14281 tgaatataag attgcctact ataatcctga aacccaggaa tcagctccag atcaggaagt
    14341 ctttaaaaag ctagagcaag cagctcagcc acaagtacag aattcaaaag aaaaggaaaa
    14401 atctgaagag gaaaagaacc attcggatca aaagaatctc cctcagacag gagaaggtca
    14461 gtcaatcttg gcaagtctag ggttcttgct acttggggcg ttttatttat tccgtagagg
    14521 aaagaacaac taatttgttc atcatctaga tgaattacat gaaactgtgg aaaatagaaa
    14581 aaatccgaag aaggccaaat atcttcggat ttttctttta aaaaaggata agttgatact
    14641 tatccttttt acttcattat gtgactatag cgttttgaaa ctagaatagg acatcataac
    14701 tgctaaaaga tttctataaa ttcatttgat tttcctaatc aatttattcg tatcctattt
    14761 cactctacta tattagtccg tctcgcacct cacggagcga gacaaaacaa
//
LOCUS       DAVBHS010000038        14733 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVBHS010000038 DAVBHS010000000
VERSION     DAVBHS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14733
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.038"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            244..1110
                     /locus_tag="VQ651_000845"
     CDS             244..1110
                     /locus_tag="VQ651_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2753866.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYQVNHLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAYFLYKLEENMSMT
                     LKVEHYIADITE"
     gene            1447..2004
                     /locus_tag="VQ651_000846"
     CDS             1447..2004
                     /locus_tag="VQ651_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase family
                     flavoprotein"
                     /protein_id="HET2753867.1"
                     /translation="MDKKNLLVLVTGSVGASNVDTYLYYIKKFYNVKVILSENSKKFI
                     SKELISYFCDKVYDEIFVEEVVPHVFLPYESDLLLILPATANVIGKIANGIADDLVTA
                     TVLNFNKKIIFCPNMNSTMWDNHIVQRNVSILKELGHIFLFESKKTYEVGLRKAIDST
                     CSMLQPQSLVKELIKLENIVLEEGH"
     gene            2225..3331
                     /locus_tag="VQ651_000847"
     CDS             2225..3331
                     /locus_tag="VQ651_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET2753868.1"
                     /translation="MINKKIQQVVLESLQNFLNGNFISPCVVYDFGLLETVLDEFKNQ
                     IPVTFNYQLFYAVKANSNEKILEFLVDKIDGVDVASLSELDVAKKFFPPTQISVNGPA
                     FSYETLYNLIKKQYKVDINFLEHLQQFSPKESVGIRVTEPDELNNRMSRFGINICSDN
                     WTSNLQNPLITRLHFHFGEKDDKFIVKLDKILFKLQEINKLREVREINLGGGFMKLFM
                     ENRLKEFFLSLMEIYKKYDIDSTVTTIIEPGSAITSFSAYMITSPVNVSEVNEQQVIT
                     LDTSIYTNTLWFVPHIITTLNSSSKERYSTILYGNTCYEHDKYKMKVSLPRLTQNSSI
                     VFFPVGAYIKSNHSNLHRNDFMREVYLWTKNLTY"
     gene            3307..4698
                     /locus_tag="VQ651_000848"
     CDS             3307..4698
                     /locus_tag="VQ651_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753869.1"
                     /translation="MDKKLDILDKVKEYLGNKTTQILDNQYKEFLKLNDIRRAFGISE
                     KVLNNSFNFTSKEFNDLINNENYLFEYACRIREEWRKKCFNHSYRFLCSPIITDDFLN
                     TKTLRSSQIEYKYERYLSKSSIGDRAVDGFVSFNTLTANGMSAIKLCLEILNSIFFKK
                     KIDLLYSTGYYETRFLLNNLAKSGISCYEVSNCELDKDKFYNVFMMEPNRADLTLQKT
                     DFKIVEYFVKYKNNSIKVVILDISYQGSNFKLVEFLEKFKFANVIIFVVRSLIKLDQM
                     GLELTNGGIIEVFIPNHLRKLKNFIEEEFNKFRNSHGANLSLYEYCLLDNSLTLKNDW
                     NYSDLVMKFTSNFYADIKDLFMENSDIEIIHEEGVPFVFLDLIGEGKKEYEMFFQWLN
                     FFYKQLGITLYARNSFGFRNLTVEYFGIIGTERYIFKICPGVYKGLSYYLMKFLLKSF
                     SNEYLKTTDEVNR"
     gene            4695..5873
                     /locus_tag="VQ651_000849"
     CDS             4695..5873
                     /locus_tag="VQ651_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2753870.1"
                     /translation="MKNLIKLLIIRLIVNLADSVFYIVALWHVSNNYSSSMFLGIFIA
                     VNYLPDLLLIFFGPVIDRVNPQKILIISILVQLAVAVIFLLLLNQISFWVIMSLVFIS
                     VMASSISYVIEDVLIPQVVEYDKIVFANSLFSISYKVLDSIFNSFASFLQVAVGFILL
                     VKIDIGIFLLALFILLLLKFRTSNANIENFSFKYYKREVLQGTKFILNNKLLFKTSIS
                     LTLINFFYSFQTVVVPIFSIRYFDGPIFYGIFLTIAGLGGILGNMLAPIVIKYLKSNQ
                     IVGVFLFLNGSSWLVAIVIKDYTLSLILFFVCFMSKGVFNIIFNSLYQQIPPHQLLGR
                     VNTTIDSIISFGMPIGSLVAGTLIDLNIELVLIAISIPYFLFSYIFYTDNGLKEFSIY
                     "
     gene            6298..6531
                     /locus_tag="VQ651_000850"
     CDS             6298..6531
                     /locus_tag="VQ651_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2753871.1"
                     /translation="MISIDLGGTNIKITVLSNDGEIETLWSITTDTSEKGSQIISDII
                     SSIKNKLTERNIPDSDLLGIGMGSCSSYFPCKS"
     gene            6814..8763
                     /gene="mutL"
                     /locus_tag="VQ651_000851"
     CDS             6814..8763
                     /gene="mutL"
                     /locus_tag="VQ651_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET2753872.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKIQITDNGYGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(9088..9555)
                     /locus_tag="VQ651_000852"
     CDS             complement(9088..9555)
                     /locus_tag="VQ651_000852"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET2753873.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(9556..10761)
                     /locus_tag="VQ651_000853"
     CDS             complement(9556..10761)
                     /locus_tag="VQ651_000853"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET2753874.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(10781..11416)
                     /gene="ribE"
                     /locus_tag="VQ651_000854"
     CDS             complement(10781..11416)
                     /gene="ribE"
                     /locus_tag="VQ651_000854"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET2753875.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(11401..12501)
                     /gene="ribD"
                     /locus_tag="VQ651_000855"
     CDS             complement(11401..12501)
                     /gene="ribD"
                     /locus_tag="VQ651_000855"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET2753876.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(12636..12750)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12907..13500
                     /gene="ruvA"
                     /locus_tag="VQ651_000856"
     CDS             12907..13500
                     /gene="ruvA"
                     /locus_tag="VQ651_000856"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET2753877.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            13510..14073
                     /locus_tag="VQ651_000857"
     CDS             13510..14073
                     /locus_tag="VQ651_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET2753878.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRDFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            14070..>14733
                     /locus_tag="VQ651_000858"
     CDS             14070..>14733
                     /locus_tag="VQ651_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2753879.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     VGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAI"
BASE COUNT         4637 a         2396 c         2761 g         4939 t
ORIGIN      
        1 gaaaggggct gaaagaacta ggggaacagt tgtaattaga aggtgatagg tatgaaaagt
       61 ggcgttgaag ctggataagt ttataaagag tttgaatgaa gaaatgaaaa gtaaataagt
      121 tgatgatgaa ttgcaaaaat agattttata tacaatttgt atctttcaaa tcattgattt
      181 gaattttaga tttaatcatg ttacaattaa aaatgaaatc aaatagaaga ggaagatgca
      241 aacgtgggca cactattagc aacaagatta aaaaatagac gaaaagaatt aaaaatgtct
      301 cagcgagaac tagctgaagg tatctgtaaa caggggcaaa tcagtcgatt agagagcgga
      361 gagtttactc ctggagcaga tttcttgcat gcccttgcta agaagttaaa agtcagtatg
      421 gattattttt ttgatgagca ggttgtagag aaagttgagg agttatcaga atttaagaaa
      481 ttagcacaga catttattac aaatcgcaat tatgagtctt tgaaatatat atatgaattg
      541 gagaatgtaa aagctcaccg cctatcttta gcagataaaa tctacatgga atggataaaa
      601 tctctgatag atttttattt ttacggacag aaagaagatg cgattgtacg attagagggg
      661 gtattatcac agttaagtgt atctgatttg aactatctcc aagtttcaaa caccctattc
      721 aatttttatt atgatattgg agatttaggt cgtttcaata aaattcgtga aaaactagag
      781 tatcaagtaa atcacctcaa tttgagtaca atcgaagagt tagaactatc tattaagttt
      841 aactacaacg tttgtcgtta tctatggtta cagaaaaata ttgaagaagc tattactaaa
      901 attacagcta ctataaaaca atgtaaggct tacagaacga attatctttt ggctgatttg
      961 tacttattga tgggaaatgt aagtgaaaat ttttcttcta aaagttcggt gaaagaatat
     1021 tttgagacgg catattttct atacaaactt gaggagaaca tgtcaatgac tctgaaagtt
     1081 gagcattaca ttgcagatat aacagaataa tgaatacggt gttcaagtga gcatcgtttt
     1141 tcaaacctag ttcagataca aaaaagtatc aaataaaaaa aaataaaaaa acattataaa
     1201 aacaattgac aagctaattt tgaagtgata aaataaatgc aagaaaacaa aaggagaggt
     1261 atatgagaag tttgtttaag aaaatagttg ctgtattagt tattggctta attctgttag
     1321 gaattgcgag aacacctcaa gttcataaga tggctagagg gattgatcct ggtcctgcga
     1381 attttattta gaaatcaatt tctactaatt gagtactaat tgagtattaa tgggtaaaaa
     1441 gattgtatgg ataagaaaaa tttacttgta ttggttactg gttcggtagg tgccagtaat
     1501 gtagatacat acttatatta tataaaaaaa ttctataatg ttaaagtgat actttctgaa
     1561 aattctaaaa aatttataag taaggaatta attagttatt tttgtgataa agtttatgat
     1621 gaaatatttg ttgaagaggt agttccgcac gtttttctgc catatgaatc tgacttactt
     1681 cttattttac cagctacggc aaatgtgatt ggcaaaattg ctaatggtat tgctgatgat
     1741 ttagttacag caactgtttt aaactttaat aaaaaaataa ttttttgtcc caatatgaac
     1801 tctactatgt gggacaatca catagttcaa agaaatgtat caattctaaa ggagttggga
     1861 catatatttt tatttgagtc taaaaaaaca tatgaggtag gattgcgtaa agcaatagat
     1921 tcaacatgtt caatgttaca accacagtcg ttagtaaaag aacttatcaa attagaaaat
     1981 attgtccttg aagagggaca ttaaaaacta ctgagaatat taaggagggg aaaaaatgga
     2041 aaattcatca atcgatgtag atatgctgtt ggaagaattg acacaagaag caatggtcgt
     2101 tgttgctgtt gataaggact gttaatttaa acttatggca atatatgaaa ggttactgga
     2161 tgttttaaat tatgcaggca gtagcctttt attatataca aatggataaa gtaaggataa
     2221 tacaatgatt aataaaaaaa tacaacaagt tgttttggaa tcattacaga attttttgaa
     2281 tgggaacttc atttcgcctt gtgtagtcta tgattttggc ttgctggaaa ctgtacttga
     2341 tgaatttaaa aatcaaattc ctgtaacatt caattaccaa cttttttatg ccgttaaagc
     2401 aaattcaaat gagaagatac ttgaattctt agtagataaa attgatggag ttgatgtggc
     2461 gtcattatct gaattagatg tggctaaaaa atttttccca ccaactcaaa tttctgttaa
     2521 tggtcccgca ttttcttatg aaactttata taatctgatt aaaaaacaat ataaagttga
     2581 tattaacttt ttggaacatc ttcaacaatt ttccccaaaa gaatctgttg gaataagagt
     2641 aacggagcca gatgaactta ataatcgtat gagtcgattt ggaataaata tttgcagtga
     2701 taattggact agtaatttac aaaatccttt aattacacga ctgcattttc attttggaga
     2761 aaaagatgat aaatttattg ttaagttaga taaaatatta tttaagttac aagaaattaa
     2821 taaacttaga gaggttagag aaataaatct tggaggcggt tttatgaaat tatttatgga
     2881 aaatcgtttg aaagaatttt ttctatcact tatggaaatc tataaaaagt acgatattga
     2941 tagtactgtg actacaataa tagaaccagg tagtgcaatt acttcatttt ctgcctatat
     3001 gattactagc ccagttaatg ttagtgaggt gaatgagcag caggttatca cgttagacac
     3061 atcaatatac accaatacat tatggtttgt tccgcatatt attacaacgt taaattcaag
     3121 tagtaaagag cgttatagta ctattctcta tggtaatacc tgttatgaac atgacaagta
     3181 taaaatgaaa gtttcgcttc caaggttaac tcaaaatagc agtatagtgt tttttcctgt
     3241 aggagcttat ataaaaagca atcattcaaa tttacatcgt aatgatttta tgcgggaggt
     3301 atatttgtgg acaaaaaact tgacatatta gataaagtta aggaatattt aggaaataaa
     3361 actactcaaa ttctggataa tcaatataaa gaatttttga aacttaatga tataaggcga
     3421 gcgtttggta tttcagaaaa agtattaaac aattctttta attttacgag taaagaattt
     3481 aatgatttaa ttaataacga aaattattta ttcgaatatg catgtagaat tagagaggaa
     3541 tggagaaaaa aatgctttaa tcattcttat cgttttctat gctcacctat aattacagat
     3601 gattttctta acacgaagac attgagaagt agccaaattg aatataaata tgagcgatat
     3661 ttatcgaaaa gttcgatagg cgatagagcg gttgatggct ttgtttcctt caatacttta
     3721 acagctaatg gtatgtctgc tattaaacta tgtcttgaga tattaaactc tattttcttc
     3781 aagaagaaga ttgatttatt atattcaacc ggatattatg aaacaagatt tttattaaat
     3841 aatcttgcta aatcaggtat tagttgctat gaggtaagta attgtgaatt ggataaagat
     3901 aaattttata atgtattcat gatggaaccc aatcgagccg atttaacatt acaaaaaact
     3961 gatttcaaga tagtagaata ttttgttaag tataaaaata attcaataaa agtcgttatt
     4021 ttagatattt catatcaagg ttctaatttt aaattagtag aatttttaga gaaatttaaa
     4081 tttgcgaatg taattatttt tgtggtacga tctttgataa aattagatca aatgggatta
     4141 gaattgacaa atgggggaat aatagaagtg tttattccta atcatttgag aaagttgaaa
     4201 aattttattg aagaggaatt caataaattt agaaattctc acggagctaa tctaagcctc
     4261 tatgaatact gtttgcttga taattcttta accttaaaaa atgattggaa ctattctgat
     4321 ttagttatga aatttacgag taatttttat gctgatataa aagacttgtt catggaaaat
     4381 tctgatattg aaatcatcca tgaagaggga gtaccttttg tatttttaga tttaataggt
     4441 gaaggtaaaa aagaatatga aatgtttttt caatggttaa acttctttta caaacagctt
     4501 ggaatcacat tgtatgctag aaatagtttt gggtttcgga atctaacagt agagtatttt
     4561 ggaattattg ggacagaaag atatatattt aagatttgtc caggtgttta taaagggtta
     4621 agttattatt tgatgaaatt tttattaaaa tctttttcaa atgaatattt aaaaactact
     4681 gatgaggtta atagatgaaa aatttgataa agttgctaat aattagattg attgttaact
     4741 tagcagacag tgtattttat atagtagcat tgtggcacgt tagcaataat tattcttcga
     4801 gcatgttctt aggaatattt attgcagtaa attatctacc ggatttgtta ctaatctttt
     4861 ttggaccagt tattgacaga gtaaatccgc aaaaaattct tataatatca attttggttc
     4921 aattagcagt ggctgtaata tttttattat tattaaacca aatatcattt tgggtgataa
     4981 tgagtctagt gtttatttca gtaatggcta gctccataag ttacgtgata gaagatgtgt
     5041 tgattcctca agtggtagaa tatgataaga ttgtatttgc aaattctctt tttagtattt
     5101 cgtataaagt attagattct atttttaatt cattcgcatc atttttacag gtggcagtag
     5161 gatttatttt attggttaag atagatatag gcatattttt acttgctcta tttatattgt
     5221 tgttgttaaa atttagaact agcaatgcga atatagaaaa cttctctttc aaatattaca
     5281 agagagaagt gttgcaaggt acaaagttta ttttaaataa taaattatta tttaaaacca
     5341 gtatttcttt aacgcttata aacttttttt attcatttca gacagtagtt gtaccgattt
     5401 tttctattcg atattttgat ggtccgattt tttatggtat ttttttaact attgctggtt
     5461 tgggtggtat attgggaaat atgctagcgc caatcgtaat aaaatattta aaatcgaatc
     5521 aaattgttgg tgtatttctt tttttgaacg gctcaagttg gttagtagca attgttataa
     5581 aagactatac tttatcactt attttatttt tcgtttgttt tatgtctaaa ggagtcttca
     5641 atattatttt taattcgttg taccaacaaa tacctccaca tcaacttctt ggtagggtaa
     5701 atactaccat tgattctatt atttcttttg gaatgccaat tggtagttta gttgcaggaa
     5761 cgcttattga tttgaatatt gaattagtgt taattgctat tagcatacct tattttttgt
     5821 tttcttatat tttttatacg gataatggat tgaaagaatt tagtatatat tagaaatgtt
     5881 tatgttcatt caaaagcata atgactataa ctgaaaaaga aaagtgatat ctttaaggtt
     5941 gttcttcttg gtggtgagat tcgtgagaca acccaagctt ttgtcggaaa gattaccaat
     6001 gctttgatgg ataggatgta ctttagcaag atgtttttag tggtaacggt atcgtggatg
     6061 gacgtgtaat aacctcttct ttcgaggagt attttactaa aaaactagcc ttggagcgtt
     6121 ccccagaaac ggacttactc attgactctt caaagatttg gggagaagat tttgcttcat
     6181 ctgttccttg aaaaaagtca cagcagtcat cacagacgat agtactgaac aaaactatga
     6241 agagttagaa atttatacgc aggtgattgt ataaaggatc tggaaataga taagaagttg
     6301 attagtattg acctaggtgg tacaaatatt aagattactg ttctttcaaa tgacggtgag
     6361 attgaaactt tgtggagtat tacaacagat acaagtgaga aaggttctca aattatatcg
     6421 gacatcatca gttctattaa aaataaattg accgaacgga atattcctga tagcgacctt
     6481 cttggaatcg gtatgggaag ttgctcatca tactttcctt gtaaatcata ggggctataa
     6541 actctccgtc tacttgtcct gcaacaattg aagtctgctc aaaacgccgt ccgctaatct
     6601 tttcatagac tttctccctt ttaggagcct agctttctag tttgttcttt gatttttatt
     6661 gagtatacca ctattttact ccctctggca agggactttg tctatgtgga gggattgggc
     6721 tcctatgtgg tggagctttt ctgttctttc tgaaatatgg tataatagca ctaatcaatt
     6781 tctaggaaaa tagatacgga aaggggctga aagatgtctc atattattga attgccagag
     6841 atgctggcaa accaaatcgc agctggagag gtcattgaac gtcctgccag cgtggtcaaa
     6901 gaattggtag aaaatgccat tgacgcgggc tctagtcaga ttatcattga gattgaggaa
     6961 gctggtctca agaaaatcca aatcacagat aacggttatg gaattgccca cgatgaggtg
     7021 gagttggccc tgcgtcgcca tgcgaccagt aagataaaaa atcaagcaga tctctttcgg
     7081 attcggacgc ttggttttcg tggtgaagcc ttgccttcta ttgcgtctgt tagtgtcttg
     7141 actctgttaa cggcggtgga tggtgctagt catggaacca agttagtcgc gcgtgggggt
     7201 gaagttgagg aagtcatccc agcgactagt cctgtgggaa ccaaggtttg tgtggaggat
     7261 ctctttttca acacgcctgc ccgtctcaag tatatgaaga gccagcaagc ggagttgtct
     7321 catatcattg atattgtcaa ccgtctgggc ttggcccatc ctgagatttc ttttagcttg
     7381 attagtgatg gcaaggaaat gacgcggaca gcagggactg gtcaattgcg ccaagcaatc
     7441 gcagggattt acggtttggt cagtgccaag aagatgattg aaattgagaa ctctgaccta
     7501 gatttcgaaa tttcaggttt tgtgtccttg cctgagttga ctcgggctaa ccgcaattat
     7561 atcagcctct tcatcaatgg tcgttatatc aagaacttcc tgctcaatcg tgctattttg
     7621 gatggtttcg gaagcaagct tatggttgga cgttttccac tggctgtcat tcacatccat
     7681 atcgaccctt atctagcgga tgtcaatgtg catccaacta agcaagaggt gcggatttcc
     7741 aaggaaaaag aactgatgac cctggtttca gaagctattg caaatagtct caaggaacaa
     7801 accttgattc cagatgcctt ggaaaatctt gccaaatcga ctgtgcgcaa tcgtgagaag
     7861 gtggagcaaa ctattctccc actcaaagaa aatacgctct actatgagaa aactgagccg
     7921 tcaagaccta gtcaaactga agtagctgat tatcaggtag aattgactga tgaagggcag
     7981 gatttgaccc tgtttgccaa ggaaaccttg gaccgattga ccaagccagc aaaactgcat
     8041 tttgcagaga gaaagcctgc taactacgac cagctagacc atccagagtt agatcttgct
     8101 agcatcgata aggcttatga caaactggag cgagaagaag catccagctt cccagagttg
     8161 gagtttttcg gacaaatgca cgggacttat ctctttgccc aagggcgaga tggactttac
     8221 atcatagatc agcacgctgc tcaggaacgg gtcaagtacg aggagtaccg tgaaagcatt
     8281 ggcaatgttg accagagcca gcagcaactc ctagtgccct atatctttga atttcctgcg
     8341 gatgatgccc tgcgtctcaa ggaaagaatg cctctcttag aggaagtggg cgtctttcta
     8401 gcagagtacg gagaaaatca atttattcta cgtgaacatc ctatttggat ggcagaagaa
     8461 gagattgaat caggcatcta tgagatgtgc gacatgctcc ttttgaccaa ggaagtttct
     8521 atcaagaaat accgagcaga gctggctatc atgatgtctt gcaagcgatc tatcaaggcc
     8581 aatcatcgta ttgatgatca ttcagctaga caactcctct atcagctttc tcaatgtgac
     8641 aatccctata actgtcctca cggacgtcct gttttggtgc attttaccaa gtcggatatg
     8701 gaaaagatgt tccgacgtat tcaggaaaat cacaccagtc tccgtgagtt ggggaaatat
     8761 taaaagtata aaaaagtctg ggaaaaattt tcaaaatcag aaaaacgcat aaaatcaggt
     8821 gttcaaaaac cttgatttta tgcgttttat catggaaata gttacttcat gttttcctaa
     8881 ttcttttcga aactcttttt aaaccacgtc agttttatca gtaatctcaa aacagtgttt
     8941 tgagctaatt ttgccagttt tatctgtaac atcgaagttg tgtttgacca ctctgcgact
     9001 ggtttcctag tttgctctat gattttcaca gagtattaaa ttgcgatttt gccaagtttc
     9061 tttattcgtc taaaagtaga gtctgttcta tgcgtctaat gtacgaatta ggttgaccat
     9121 ttcaatagct ccttgtgcac actcagaacc cttatttcct gctttggtac cagctcgttc
     9181 tatggcttgt tcaattgtat ctgtcgttag cacaccaaac ataacaggaa tttcgctatt
     9241 taaactgatt tgggcgattc ccttagatac ctcgctacat acataatcat aatgacttgt
     9301 attccctcta atgacagctc ccaagcagat aattgcatca tattttttac tttttgccat
     9361 ttttgatgca atcagtggta tttcaaaagc tcctggaacc caggctacct ctatatcttt
     9421 ctcgtttaca ttctctcttt tgagattatc tagtgctcca gataataatt ttgaagttat
     9481 aaattcatta aatctcgcta caacaatacc tattttaata ttgtttgcta ctaaattacc
     9541 ttcataagtg ttcatttatt tttcctccat atttaaaatg tgacccattc gatttttctt
     9601 tgtttctaaa taaaaactat cgtaaggatt ggcttctatt tcgattgata ttctactgga
     9661 aatggtaatt ccatattttt ctaactgttc aaccttgtca ggattatttg tcagtaaatg
     9721 aagtgactga agtcccagat ctttaagcat ttgtgctcca atatgatatt ctcttaaatc
     9781 accttcaaag cctaatgcaa gattggcatc aagcgtatcc atgccttgat cttgtaaatg
     9841 ataggctttt aatttattga taagtccaat tcctcgtccc tcctgtcgca agtaaagtaa
     9901 gacacccgaa ccattctcaa caatcatttt catagcttta tcgaattgct gtccacaatc
     9961 gcaacgtaaa gagcctaaaa catctcctgt taaacattcg gagtggaccc gacataatac
    10021 attggcttca tcctctatat ttcccataat aagagcaaga tgatgttccc catttagttt
    10081 atctatatag ctaattgctt tgaaattacc gtatctagta ggcatattga cagttgaaac
    10141 tcgttctacc agctgatcat atacttttct atattcttgt aattctttga tggtaattag
    10201 tggaatgttg tgtttcttcg agaactgaat taaatcatct gttctcatca ttttgccatc
    10261 atgattcatt atttcacaac ataggccaca ctcttttagt ccagctaatt ttaataaatc
    10321 aacagttgct tctgtgtgtc catttctttc tagaacacca cctttttttg caattaaagg
    10381 aaacatgtgt cctggcctgc gaaaatcaga gggtgttata tcttcagcta cacacatacg
    10441 tgcggtcagt cctctctcct cggcagaaat acctgtggtc gtttctttat aatcaattga
    10501 aactgtaaaa gcagtcttat gattatctgt attgttttca accataggtg aaagcattaa
    10561 ttgattagct aaactttcgc tcataggcat acaaattaat cctttggcat aagtagccat
    10621 aaaattaaca ttttctgttg tagctgcttg tgcagaacaa attaagtctc cttcattttc
    10681 tctatccttg tcgtctataa caagaacaag tcgtcccttc tgcaatgctt ctaatgcttc
    10741 ttgtattttt cgatattcca ttgactgatt atcctttctg ctaaaatcca ttttgatata
    10801 atagttcctt agatatttct gattttggag agttatccat cagtttttgc acatatttac
    10861 ctaagatatc attttcaaga tttactgtac tcccgacttg tttactctta agaatggttt
    10921 gttccaaggt atgagggata acagatactg aaaagtttac tttggagact ttagcgacag
    10981 tcagactaat gccgtcaatt gtaatagatc ctttttcaac tattaaatct aaaatttctt
    11041 tttgtgtgtt gatttgatac catacagcat tatcatcttt ttttattgac gagatttttc
    11101 ctgtaccatc aatgtgtcct gtaacgacgt gacccccaag tcgaccgttg acagataagg
    11161 ctctttctag attcacctca cttccatgtt ttaatagagt aagagctgtt cgactccatg
    11221 tttcattcat tacatcaact gtaaaggatt gatgattgaa atgagtaact gtaagacaga
    11281 taccatttac tgctatacta tcgcctaaat ggatatccgt taatattttt gaggctttaa
    11341 ttgatagttt acaattacga gagtctttct gtattctttc aacttttccg atttcttcaa
    11401 ttattcctgt gaacatggat aaatcacttc actttctatg agatagtcat ttcctatttg
    11461 agaaaaagca taaggtttca atctaatagc gtcatttggc aaagaaatgc cttcacctcc
    11521 gacaggaaac ttggcactgc ctccaaaaat ttttggtgca atatatattt tcagctcatc
    11581 aacaatttgt tgttccaaag cactccaatt cattagactg cccccttcta gaactaggct
    11641 atcaatctgc atgtttccta gatgttgcat taaactcgat aagtctatat gattgccttt
    11701 tttctttatg gaaagtattt cacagccatg attttgatat agcttcattt tatttttgtc
    11761 ttcagaggaa gtggcaatgt aagttttaat atcatttgct gtttttacga ttttagaggt
    11821 aagaggagtt cgtaaatgtg tatcgcatat gatacggata ggatttttcc cttcctccaa
    11881 tctacatgtc agcaaaggat cgtcttgaat aacagtattg actcccacca taattgcact
    11941 aacatggtgt cgtaactgat gcacatgctt tcttgcttct tcttcagtaa tccatttgga
    12001 ttgatttgtt ttagtggcta tttttccatc cattgacatt gcatatttca taaaaacata
    12061 gggtacatgc tgggtaatat actttctaaa actttttatt aagttaagac actcattttc
    12121 taaaattcca acagtaactt gaagattatt ttcctcaagt atctttactc cttttccaga
    12181 tacaatagga ttacagtcta ggcttccaat gactactctt gtaataccac tatcgattat
    12241 agcatctata cagggaggtg ttttcccgaa gtgacaacag ggttcaagtg ttacataaag
    12301 cgtcgctccg acaggggatt ctctacagtt tttaagagca tttctctcag catgtgggcc
    12361 accaaaaaac tcatgataac cttgtccgat gatgtgatta tcttttacaa taactgcgcc
    12421 gaccatagga ttgggattga cgtaaccagc ccctttttgt gccagtttta ttgctaattt
    12481 catatatttt gaatcgctca tctcactacc tccaaaaaaa atataccttg aataggggac
    12541 tactcaaggc atacaaaaga aaacttatgc gattaacaaa aatactctga aatgacaagt
    12601 aatcatttca gagcacgcaa aaagcacaaa tatactttta tcttctttca tccagactat
    12661 actgtcggct ttggaatttc accaaatcat gcctttcggc tcgtgggcta taccaccggt
    12721 agggaattac accctgccct gaagatagtt attcaattac agatgattat agtacttaat
    12781 tttgaatatg tcaacagata aataccgatt gtttttgata tactgtattt gtgataatcg
    12841 attctcgctc ctcggataaa gaaaatatga tatactagat aaacgaaata agagagaagg
    12901 aatactatgt acgcatattt aaaaggaatc attaccaaaa ttactgccaa atacattgtt
    12961 cttgaaacca atggtattgg ttatatcctg catgtggcca atccttatgc ctattcaggt
    13021 caggttaatc aggaggctca gatttatgtg catcaggttg tgcgtgagga cgcccatttg
    13081 ctttatggat ttcgctcaga ggatgagaaa aagctctttc ttagtctgat ttcggtctct
    13141 gggattggtc ctgtatcagc tcttgctatt atcgctgctg atgacaatgc tggcttggtt
    13201 caagccattg aaaccaagaa catcacctac ttgaccaagt tccctaaaat tggcaagaaa
    13261 acagcccagc agatggtgct ggacttggaa ggcaaggtag tagttgcagg agatggcctt
    13321 cctgccaagg tcgcagtgca agcaagtgct gaaaaccaag aattggaaga agctatggaa
    13381 gccatgttgg ctctgggcta caaggcaaca gagctcaaga aaatcaagaa attctttgaa
    13441 ggaacgacag atacagctga gaactatatc aagtcggccc ttaaaatgtt ggtcaaatag
    13501 gagcagagaa tgacaaaacg ttgttcgtgg gtcaagatga ccaacccact ctacatcgcc
    13561 tatcatgatg aggagtgggg ccagcccctc catgatgacc aagcgttgtt tgagttattg
    13621 tgtatggaaa cctatcaggc aggcctgtct tgggaaacgg tactcaacaa acgccaagct
    13681 ttccgagaag cctttcatag ctatcaaatt cactcagtcg cagagatgac tgacactgaa
    13741 ttggaagcca tgctggagaa tccagctatc attcgaaata gagccaagat ttttgctaca
    13801 cgcgctaacg cccaagcttt tctacagtta caggcagagt acggctcttt tgatgcctat
    13861 ctttggtctt ttgttgaggg gaaaactgtc gttaacgatg ttcctgatta tcgccaagcc
    13921 ccagctaaaa cacccttatc tgagaaatta gccaaagatc tcaaaaaacg agacttcaag
    13981 ttcacaggcc cagtcgccgt attgtctttt ctacaggctg cagggctagt tgatgaccac
    14041 gagaatgatt gtgagtggaa aggtcttaaa tgatgtctaa caaaaataag ggaattctga
    14101 tttttgcgat tctctataca gtcctctttg tgtttgatgg cgttaaattg ctggcttctt
    14161 taatgccatc tgccattgca aattatcttg tttatgtagt tttagctcta tatggctcct
    14221 tcttattcaa ggatagattg atccaacaat ggaaggagat tagaaagact aaaagaaaat
    14281 tcttctttgg cgtcttaaca ggatggctct ttctcattct gatgactgtt gtctttgaat
    14341 ttgtatcaga gatgttgaag cagtttgtgg ggctagtcgg acaaggtcta aatcagtcta
    14401 atattcaaag cacttttcaa gagcaaccac tactgatagc tgtttttgct tgtgtcattg
    14461 gacctctggt agaagaatta tttttccgtc aggtcttatt gcattacttg caggaacggt
    14521 tgccaggttt actaagcatt attctggtag gacttgtttt tgctctgact catatgcaca
    14581 gtttggctct atcagagtgg attggtgcag ttggttactt aggtggaggc cttgcctttt
    14641 ctattattta tgtgaaagaa aaagagaata tctactatcc actacttgtt cacatgttaa
    14701 gcaacagcct ctccttaatc attttagcta tca
//
LOCUS       DAVBHS010000039        14353 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVBHS010000039 DAVBHS010000000
VERSION     DAVBHS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14353
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.039"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            60..716
                     /locus_tag="VQ651_000859"
     CDS             60..716
                     /locus_tag="VQ651_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET2753880.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            706..1029
                     /locus_tag="VQ651_000860"
     CDS             706..1029
                     /locus_tag="VQ651_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET2753881.1"
                     /translation="MVSKYLLLAVIFSDLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAV
                     LRLVF"
     gene            1133..1963
                     /locus_tag="VQ651_000861"
     CDS             1133..1963
                     /locus_tag="VQ651_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2753882.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NSSPKPFNYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYQMAV
                     NNISYTKERAEKYLYAAPTVKDPNVLVVKKDDSSIKSLDDVGGKSTEVVQGTTSAKQL
                     EDYNKQHSDNPTILNYTKANLQQIMGHLSDGQFDYKISDKITVETVIKNQGLDNLKVI
                     DLPSDQQPYVYPLLAKGQDELKTFVDKRIQELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2004..2081
                     /locus_tag="VQ651_000862"
                     /pseudo
     CDS             2004..2081
                     /locus_tag="VQ651_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC protein"
     gene            2185..3015
                     /locus_tag="VQ651_000863"
     CDS             2185..3015
                     /locus_tag="VQ651_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2753883.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPKPFIYEENGELTGYEIEVVRAIFKDSDKYNVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            3169..4023
                     /locus_tag="VQ651_000864"
     CDS             3169..4023
                     /locus_tag="VQ651_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HET2753884.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            4123..5496
                     /locus_tag="VQ651_000865"
     CDS             4123..5496
                     /locus_tag="VQ651_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET2753885.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            5489..6550
                     /locus_tag="VQ651_000866"
     CDS             5489..6550
                     /locus_tag="VQ651_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HET2753886.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            6552..7244
                     /locus_tag="VQ651_000867"
     CDS             6552..7244
                     /locus_tag="VQ651_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2753887.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(7274..7843)
                     /locus_tag="VQ651_000868"
     CDS             complement(7274..7843)
                     /locus_tag="VQ651_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET2753888.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            7978..8592
                     /locus_tag="VQ651_000869"
     CDS             7978..8592
                     /locus_tag="VQ651_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET2753889.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            8594..10240
                     /locus_tag="VQ651_000870"
     CDS             8594..10240
                     /locus_tag="VQ651_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2753890.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDR
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHRASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            10252..11538
                     /locus_tag="VQ651_000871"
     CDS             10252..11538
                     /locus_tag="VQ651_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2753891.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGCD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            11599..12132
                     /locus_tag="VQ651_000872"
     CDS             11599..12132
                     /locus_tag="VQ651_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2753892.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRTAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKASAEEEETEELA"
     gene            12210..12680
                     /gene="nrdI"
                     /locus_tag="VQ651_000873"
     CDS             12210..12680
                     /gene="nrdI"
                     /locus_tag="VQ651_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HET2753893.1"
                     /translation="MKIISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(12973..14235)
                     /locus_tag="VQ651_000874"
     CDS             complement(12973..14235)
                     /locus_tag="VQ651_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET2753894.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFSGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         4254 a         2680 c         3264 g         4155 t
ORIGIN      
        1 aagacaatat gaggagaaag atgaaagaaa aaggatttta ggagggggcg caggcagcca
       61 tgccaacggc tcttggctat gtcagtatcg gcctggcctg tgggactatc ggtgcgccct
      121 atgtgacacc tgttgagatg ggcttgatga gtctctttgt ttatgctggg agtgcccagt
      181 ttgccatgtt ggcactgatt gtggttcaag ctcctgtggc agctattgct atgacggttt
      241 ttctaatcaa cttgcgtctc tttttgttga gtttacacgc atcgacttat ttccgtcata
      301 ccagtctttg gtacaatatc ggtatgtcta gtatcttgac agatgagacc tatggcgttt
      361 tgatgggtga attggcccat acagacaagg taaatcctat gtggatgcac ggaaacaatc
      421 ttaacagcta tgtggcttgg tttgtgggga cagtagtcgg aacggctctg ggtggcctgc
      481 taccaaatcc agaaatcttt ggcttggatt ttgccctggt tgggatgttt attggtattt
      541 ttgcttcgca atttcagatt atgcaaagac ggattcctgt ccgcaatctg ctcattatcc
      601 tagcagttgt tgcggtgtcc ttctttttgc tcttgacagt ggtgtctcag tcactagctg
      661 ttctgtttgc gacgctactt ggttgtagca tgggggtggt tttagatggt cagtaagtat
      721 cttttattag cagttatttt ctctgacttg gtgacttgga ttctccgtat gattcccttc
      781 atcttagtca agtataaggg cttgcctgca atcgttgagc gttttttgaa gttcttgccc
      841 gtttccatta tctttgcctt gattctttca agcgtagtga caggtaaggt tgggagcctt
      901 cctcaaatta aatggctaga cttcttagcc gtctttccaa cagcttgggt agcctttcgc
      961 taccgcaatc tagtcggaac agttctcttt ggagtagtct tgattgccgt cttgcgtttg
     1021 gtcttttaaa tcagccataa agaaaaccta tcacagatat atatatcata taatggtgta
     1081 aatgattttt ttctgttaag attataaggt attctatttt ggaggaaatg acatgaaaaa
     1141 aatcgttaaa tactcatctc ttgctgccct agggcttgtt gctgcaggtg tgctagcagc
     1201 ttgctccggt ggtgctaaga aagaaggaca agcagctagc aagaaagaaa ttattgttgc
     1261 aactaattct tcaccaaaac cattcaacta tgaagaaaat ggcgaattga ctggttacga
     1321 gattgaagtc gttcgcgcta tctttaaaga ctctgataag tatgatgtca agtttgagaa
     1381 gacagagtgg tcaggtgtct ttgcgggtct tgacgctgac cgttaccaaa tggctgttaa
     1441 caacatcagc tacactaaag aacgtgcgga gaaatacctt tatgcagccc caactgttaa
     1501 agaccctaac gttcttgtag tcaagaaaga cgattctagc atcaaatcgc ttgatgatgt
     1561 tggtggaaaa tcaacagaag ttgttcaagg aacaacatct gctaaacagt tagaagacta
     1621 caacaaacaa cactcagata atccaactat ccttaactat actaaagcaa acttacaaca
     1681 aatcatgggc cacttgagcg atggtcagtt tgactacaag atttctgata aaatcactgt
     1741 tgaaacagtc atcaagaacc aaggtttgga caacttgaaa gtcatcgatc ttccaagcga
     1801 ccaacaacct tacgtttacc cacttcttgc taaaggtcaa gatgagttga aaacatttgt
     1861 agacaaacgt atccaagaac tctacaaaga cggaactctt gaaaaattgt ctaaacaatt
     1921 cttcggagac acttacctac cggcagaagc tgatattaaa taatttcttg aaatcatcca
     1981 ttctgagtaa gatgggtgat ttttcagttc gctaccgcaa tctagtcgga acagttctct
     2041 ttggagtggt cttgattgcc atcttgcgtt tggtctttta aattacccat aaaaaaaact
     2101 tatcacagag atagatatca tataatggcg taaatgctct ttttctgtta agattataag
     2161 gtattctatt ttggaggaaa tgacatgaaa aaaatcgtta aatactcatc tcttgcagcc
     2221 cttgctcttg ttgctgcagg tgtgcttgcg gcttgctcag gtggtgctaa gaaagaagga
     2281 caagcagcta gcaagaaaga aatcatcgtt gcaaccaatg gatcaccaaa gccatttatc
     2341 tatgaagaaa atggcgaatt gactggttat gagattgaag tcgttcgcgc tatctttaaa
     2401 gattctgaca aatataatgt caagtttgaa aagacagaat ggtcaggtgt ctttgctggt
     2461 cttgatgctg accgttacaa tatggctgtc aacaatctta gctacactaa agaacgtgcg
     2521 gagaaatacc tctatgccgc accaattgcc caaaatccta atgtccttgt agtgaagaaa
     2581 gatgactcta gtatcaagtc tctcgatgat atcggtggaa aatcgacgga agtcgttcaa
     2641 gccactacat cagctaagca gttagaagca tacaacgctg aacacacgga caacccaact
     2701 atccttaact atactaaggc agatttccaa caaatcatgg tacgtttgag cgatggacaa
     2761 tttgactaca agatttttga taaaatcggt gttgaaacag tgatcaagaa ccaaggtttg
     2821 gacaacttga aagttatcga acttccaagc gaccaacaac cgtacgttta cccacttctt
     2881 gctcagggtc aagatgagtt gaaatcattt gtagacaaac gcattaaaga actctacaaa
     2941 gacggaacgc ttgaaaaatt gtctaaacaa ttcttcggag acacttatct accggcagaa
     3001 gctgatatta aataatttct tgaaatcatc cattctgagt aagatgggtg gtttctcagt
     3061 ttttagggat atagttttaa actatatatc tgactatcta ataacaattt gtgaaatcaa
     3121 acaaattgtg agatactagt acggtattat ttttaaggag aaagaatcat gaaaatcaaa
     3181 aaatggcttg gcctagcagc ccttgctaca gtcgcaggtt tggctcttgc agcttgcgga
     3241 aactcagaaa agaaagcaga caatgcaaca acgatcaaaa tcgcaactgt taaccgtagc
     3301 ggttctgaag aaaaacgttg ggacaaaatc caagaattgg ttaaaaaaga cggaattacc
     3361 ttggaattta cagagttcac agactactca caaccaaaca aagcaactgc tgatggcgaa
     3421 gtagatttga acgctttcca acactataac ttcttgaaca actggaacaa agaaaacgga
     3481 aaagaccttg tagcgattgc agatacttac atctctccaa tccgccttta ctcaggtttg
     3541 aatggaagtg ccaacaagta cactaaagta gaagacatcc cagcaaacgg agaaatcgct
     3601 gtaccgaatg acgctacaaa cgaaagccgt gcgctttact tgcttcaatc agctggcttg
     3661 attaaattgg atgtttctgg aactgctctt gcaacagttg ccaacatcaa agaaaatcca
     3721 aagaacttga aaatcactga attggacgct agccaaacag ctcgttcatt gtcatcagtt
     3781 gacgctgccg ttgtaaacaa taccttcgtt acagaagcaa aattggacta caagaaagca
     3841 cttttcaaag aacaagctga tgaaaactca aaacaatggt acaacatcat tgttgcgaaa
     3901 aaagattggg aaacatcacc taaggctgat gctatcaaga aagtaatcgc agcttaccac
     3961 acagatgacg tgaaaaaagt tatcgaagaa tcatcagatg gtttggatca accagtttgg
     4021 taataagaaa cagggaggtg ggagagaaaa ttccacctct tgcttttgta tagagtatag
     4081 attgtaaaga agactattcg ttcatagaaa ggtagagaga atatggtttt tcctagcgaa
     4141 caagaacaga ttgaaaaatt tgaaaaggat catgtagtcc aacattattt tgaggttttg
     4201 cgtaccttga tttctaagaa atcagtcttt gcccagcagg ttggactcaa ggaagtcgca
     4261 aattatctgg gtgagatttt caagcgtgtt ggagctgaag tggagattga tgagagctat
     4321 acagcgccct ttgtcatggc acatttcaag agttcgcgtc cagatgccaa gaccttgatt
     4381 ttctataacc actatgacac tgtgccagcg gatggggatc aggtctggac agaggatcct
     4441 tttacgcttt cggtccgcaa tggcttcatg tatgggcgtg gggttgatga cgacaagggt
     4501 catatcacag ctcgcttgag tgctttgaga aaatatatgc agcaccatga tgatttacct
     4561 gtcaatatca gctttatcat ggagggagcg gaggaatcgg cttcaacaga cctagataag
     4621 tatttggaaa agcatgcaga caaactccgt ggggcggatt tgttggtctg ggaacaaggg
     4681 accaaaaatg ccttggaaca gctggaaatt tctggtggca ataaggggat tgtgaccttt
     4741 gatgccaagg taaaaagcgc tgatgtggat atccactcga gttatggtgg tgttgtggaa
     4801 tcagctcctt ggtatctcct ccaagcctta cagtctcttc gtgctgcgga tggccgtatc
     4861 ttggttgaag gcttgtacga agaagtacaa gagcccaatg aacgagaaat ggccttgcta
     4921 gaaacttatg gtcaacgaaa cccagaggaa gttagtcgga tttatggatt ggagttgcct
     4981 ctcttacagg aggagcggat ggcctttcta aaacgtttct ttttcgagcc agcgcttaat
     5041 atcgaaggaa tccagtctgg ttatcaaggt cagggtgtta agactatttt acctgcagaa
     5101 gccagtgcca agctagaggt tcgtctggtt ccgggcctag aaccgcatga tgttctggaa
     5161 aaaattcgga aacaactaga caaaaatggc tttgataagg tagaattata ctataccttg
     5221 ggagagatga gctatcgaag cgatatgagc gcaccagcca ttctcaatgt gatcgagttg
     5281 gccaagaaat tctatccaca gggcgtttca gtcttgccga cgacagcggg gacaggacct
     5341 atgcatacgg tctttgatgc cctagaggta ccaatggttg catttggtct aggaaatgcc
     5401 aatagccgag accacggtgg agatgaaaat gtgcgaatcg ctgattatta cacccatatc
     5461 gaattagtag aggagctgat tagaagctat gagtagagat attatcaagt tagatcagat
     5521 cgatgtgact tttcaccaaa agaagagaac catcacagcg gttaaggatg tgaccattca
     5581 catccaagaa ggggatatct acggaatcgt tggatattct ggagcaggga aatcaaccct
     5641 tgtacgggtg attaacctct tgcaaaaacc atctgcaggg aaaattacca ttgacgacga
     5701 tgtgattttt gatggtaagg tcaccttgac ggcagagcag ttgcgtcgta aacgtcaaga
     5761 tatcgggatg attttccagc attttaacct gatgagccaa aagacagcag aggagaatgt
     5821 agcctttgcc cttaaacact ctggactcag caaggaagaa aagaaggcta aagtagctaa
     5881 gttgttggac ttggttggtt tggcagatcg tgctgaaaac tacccttcac aactatctgg
     5941 agggcaaaaa cagcgtgtgg caattgcgcg tgccttggcc aatgatccaa aaatcttgat
     6001 ttcagacgag tcaacttctg cccttgatcc gaagacaacc aagcagattt tggccttgtt
     6061 gcaagatttg aaccaaaaat taggcttgac tgttgtcttg attacgcatg aaatgcagat
     6121 tgtcaaagac attgccaacc gtgttgcagt tatgcaggat gggcatttga ttgaagaggg
     6181 tagtgtgctt gaaatcttct caaatcctaa acaacctttg actcaagact ttatctcaac
     6241 agctacaggt attgacgaag ccatggtcaa aatcgagaag caagaaatcg tggaacactt
     6301 gtctgaaaac agtctcttgg tgcaactcaa gtacgctgga gcttcaacag acgagccact
     6361 tttgaatgaa ttgtacaagc attaccaagt aatggctaat attctctatg ggaatatcga
     6421 aattctcgat ggtactcctg ttggagaatt ggtggtggtc ttgtcaggtg aaaaagcagc
     6481 gttggcaggt gcccaagaag ccattcgtca agcaggtgta caactaaaag tattgaaggg
     6541 aggacagtaa gatggaatca ttgattcaaa cctatttacc aaatgtctat aaaatgggtt
     6601 gggctggtca ggcaggctgg ggaacggcta tctacttaac tctttatatg acagttcttt
     6661 ccttcattat cggaggcttc ttggggctag tggcaggtct ctttctcgtc ttgacagcgc
     6721 caggtggtgt cttggagaat aaagtcgtat tctggatttt agacaaaatt acctcaattt
     6781 ttcgtgcagt tccctttatc atcctcttgg caatcttgtc accactttct cacttgattg
     6841 ttaaaacaag tatcgggcca aatgcagccc ttgtcccact ttcttttgca gtctttgcct
     6901 tctttgcccg tcaggtgcag gttgtcttgg ctgaattgga tggcggtgtc attgaggcgg
     6961 ctcaagcgag cggagcgact ttctgggaca tcgtgggtgt ttacctatca gaaggtcttc
     7021 cagatttgat ccgtgtgacg actgtgacct tgatttccct tgttggggaa acagctatgg
     7081 ccggtgcggt tggagctggt ggtatcggta acgtagccat cgcttatgga tttaaccgct
     7141 acaatcacga tgtgaccatc ttggcaacca tcattatcat tttgattatc tttgcaatcc
     7201 aattcttggg agatttcttg actaagaaat tgagccataa ataaaaaaga gccgcgtggc
     7261 tctttttaac tgatcagatt ttctgtgcaa attttttact caaggcttgt ccaatcaagg
     7321 cacccactag ggctccgatg acaatacttg cgataaatag aaggacagtt ccagggtttg
     7381 gagcgaccat gatgcggtcg atatattctt gggattttcc tcttgccaga agagtagcca
     7441 tataggcttt gggcgcaatc cacataagca agattggtcc tgttgtacta aaggcgaaaa
     7501 taatgaaaga aaggaagttc tttgttttgt ccttgtattt tcctaaatga gctactccat
     7561 ctgctaggag gccacagata attccaggaa ggaaggaacc agcaccgtgt ttagttccca
     7621 agaaaaagag ggcaatgaca aggccgatag tggtaatggc tccaaagcgc ggaacttttg
     7681 cgactaggat catatagacg ctaccgccga caagggcagt aaaggcaggc gcataaaaca
     7741 tgtttccagt ttggtcaaag agattgccca aaaggacacc aatccccatg cagaggaagt
     7801 aaagaactgc aaaaagcagt gtagttaaga tagatttctt catgaattgt ctcctgataa
     7861 ttttttcaca attctcattt taccatggtt tggtgggatt gtaaatggat actagaagtt
     7921 tttgaaaacg cttgaaatat gatataatgg tttcatagtt atttttaggg gaatatcatg
     7981 ggtagatttt tagactttgt ctttaatcgt ttctttttag ggatgattgc gacagccttc
     8041 ttttggctat taactttagc aggagggatt atccttggtc tagcgccggc tagtgccacc
     8101 ttgatgagct tatatgcaga acatggttat agctttcggg aatacagttt gaaggaggct
     8161 tggtctcttt acaagcaaaa ttttgtctca agcaacctga ttttctatag ctttttaggt
     8221 gtgggtctag ttttgaccta tggtttgtat ctcttggtgc aattgcctca tcagactatt
     8281 gttcatttga ttgcgaccct tttgaatgtc ctagtagttg ccctgatctt tttggcttat
     8341 acagtatctt taaaattaca agtttatttt gccttgtcct atcgaaatag tctcaaatta
     8401 tccttgattg gcatctttat gagtctagca gctgtggcca aggttctcct tgggactgtg
     8461 ctacttgtag caattggtta ctatatgcct gccctgctat tttttgtagg aattgggatg
     8521 tggcatttct ttatcagtga tatgttggaa cctgtctatg aaatcatcca tgaaaaattg
     8581 gcgacaaaat agaatgaagc agttttggct acatacgctt ctaagaacct atagttcagt
     8641 gatgatcatt atcattgcga gttttgcaat cttactctct tacgctgact gggattcacg
     8701 tgaaaaggaa gcccagagag tagcccagcg tgtaactgct agaacagtga gtgaaattga
     8761 atattaccat agagagtcaa cccagatagc tcaggcttta gttgaaaatc aagctcgtat
     8821 tgagggaatc tataaatact ttagccttag catgccagac tatttttact ggcaattaga
     8881 gcggaaagct tcgccttata tatcagtctc tctgtatgaa aatgttgatg acctctatgt
     8941 tcgaaatgat tttgtaactg gggtggccat tgcttttcaa gattacaagg aagtctatgt
     9001 ttctactaaa gacaaacgta gtggagaaaa aatcagggct gaggatttca aaccagcagg
     9061 aaatagtttt gccattccgg tgtcagatcc agtgtcagat caagatttag gagtgattta
     9121 catctccttg gatcctgctg ttttatacca tgccattgat aatactagag gtcatactcc
     9181 gatggcagta acagtgacct caccttttga tacggagatt tttcatattg gtgagacagt
     9241 tgatagggag agtgaaaatt ggctagttgg cttaacttct catggatatc aggttcaggt
     9301 ggcagttcct aaaaactttg ttttacaagg aacagtgact agctctgctt tgattgtggg
     9361 tttgagcctt ctctttattg tcattcttta tctgactttg aggcagactt ttgctaatta
     9421 ccaaaagcag gtagtggatt tagtggaatc cattcaagtc attgctcaag gcgaagaggg
     9481 gcgtcggatt gacatttccg aaaaagatca ggaattactc ctaatcgcgg agacgaccaa
     9541 tgatatgttg gatcgattgg aaaagaatat ccatgatatt taccagttag agcttagtca
     9601 aaaagatgcc aatatgcgag ccttgcaggc gcaaatcaat cctcatttta tgtataatac
     9661 gctggagttc ttgcgcatgt atgcagttat gcagagtcaa gatgagttgg cagatatcat
     9721 ttatgaattc agtagtctct tgcgtaacaa tatttccgac gaaagagaga ccctcctcaa
     9781 acaggaatta gaattttgcc gtaaatacag ctatctctgc atggttcgct atcccaagtc
     9841 cattgcctat ggtttcaaga tagatccaga gttagagaat atgaagattc ccaagtttac
     9901 cttgcaaccg ctggtagaaa actatttcgc gcatggtgtt gaccacaggc ggacagataa
     9961 tgtgattagc atcaaggctc ttaaacagga tggttttgtg gaaattttgg tggtcgataa
    10021 tggtagagga atgtcggctg aaaagttggc aaatatccga gaaaaattaa gtcagaaata
    10081 ttttgaacac cgagccagct acagtgatca aaggcagtct atcgggattg tcaatgtaca
    10141 cgagcgtttt gtgctctatt ttggagaccg ctatgccatt acgatagagt ctgcagagca
    10201 agccggtgtt cagtatcgta ttacaattca agatgagtag aaagggagaa aatgtataaa
    10261 gtattattag tagatgatga gtacatggtg acagaaggtc tgaagcgttt gattcccttt
    10321 gataagtggg atatggaggt cgtcgcaaca gccagtcatg ccgatgaagc tctagaatat
    10381 gttcaggaaa atcctgtcga tgtcatcatt tccgatgtca atatgccaga caaaacaggg
    10441 cttgatatga ttcgggagat gaaagagatc ttaccagatg ctgcctatat cctgctctca
    10501 ggttatcagg agtttgatta tgtaaaaaga gcaatgaacc ttagtgtggt ggactatttg
    10561 gtcaagcctg ttgataaggt agagctggga aatctgctgg agaagattgc aggtcagctc
    10621 ggcgagagag ggaagaaaag tcagactctt agtcaagaat tagacgaggc tggatttgtt
    10681 agttatttag gggataagga gaattggtgg ataggtctat ccaaggaaaa acaaggttcc
    10741 ttcaccattc cctactatgt cttgggccaa gactggcaga ttttcatttc tgaccaaccc
    10801 ctagatggtt tagtcgttac accttttgaa gctccttatc aagaacactt tgaacgctgg
    10861 aagctgaatg ctgagaaaac cctcttttac ggttctgtaa atttgcagca gtctgagagt
    10921 ctctttgcct attacgaacc gatttatagg gttatcattc agggaaatct caatcaaatc
    10981 gtagaagagt taaatctctt ggagaaggta gttcttgaaa atacgccgcg tgttccgatt
    11041 actaaacagc tttttattca gtttgtcatg gatgttttcc atttatttga acatctcaaa
    11101 gctgatgacc tgacagacat cgtcaaaacc attcatgcta ttcaatcctt cgatgaattg
    11161 gttccttata tcaaggaaac tctgagcagc tttttcggtc aataccgtat gaatgaaaat
    11221 gtggtcagtg tgctggaagt cattggttgt gattaccaaa aagagctttc cctcaaggat
    11281 atcagtaagg ctctctttat caatcctgtc tatctagggc agttgattaa gcgtgaaacc
    11341 gattcgactt ttgcagagtt actaaacaaa caacgtatta aggctgccca acaacttttg
    11401 ctttcaacta gtgacagcat cgaagatatt tgttatgctg ttggttacag taaccttgga
    11461 tatttctata aagttttccg aaaattgtgc ggaaaatcgc caaaagccta ccgaaaacag
    11521 gtagaaacta tactataaga tttgtattcc tttacaaaat gtgctataat ataaatatta
    11581 ttattaggag gttctatcat gaaaaagaaa ccaatttatc tatgggtctt gctaatcttg
    11641 tctgccctta tttcagttcc gtctctgttt ggaattgtga gtcccttgcc tagtaaagaa
    11701 gcccttcgta ctgctcagaa acaagttgca ggggtcaatg ctcagcaatt agaagaccag
    11761 cttaattata cctatagagt agcagaagca tctcattcta tttttaatgt tgctttgatt
    11821 gtgctatcta ctattttagt ggtcgtagcg attgtcttcc ttgttcgtaa aaatttgcaa
    11881 tatgcaaact atacttacgt tggctatgtt ttgttagcta ttattggttc gatttatggc
    11941 tatgttggtt tacaagacgc tgtgcagttg gttcaagatg agagcatgcg tttgacagtg
    12001 agtattggtt caaaagctgt tagcattttc tatatcgtca tcaatgttct cttcctagcc
    12061 cttgtcttct ataagatgtg gcgacaacag aaagcctcgg cagaagaaga ggaaacagaa
    12121 gagcttgcct aagtattgac aaaaaagaac tatcaagata caatcttggt agttgttttt
    12181 tgattataga taaaatcatg gaggtacata tgaagataat ttctttagtt tatattagtt
    12241 tgagtggcaa cactgaaagt tttgtgacgc gcttgaaaga ctatctcttg tcccagtaca
    12301 aagggattga ggttcaaaag attcatatca aggatttagt caaggaaggc aaaaatttct
    12361 atgaaatgga ccatccctat gtcgcttttt tgccgaccta tctcgaaggt gggaatggcg
    12421 tggataacgg agatgttgag attttgacga caccggtggg agattttatt gcctatggta
    12481 acaatgctag taagtgtttt ggtgtggttg ggtcaggaaa tcgtaacttt aataaccaat
    12541 actgcctaac agccaagcaa tacagtcaac gctttggctt tcctgtattg gctgactttg
    12601 aaatgcgagg catgctggaa gatatcaaac atgtcgcagc tattatcgca gatttgtatg
    12661 agttggaaaa ggagaattaa tactcaatga aaatcaaaga gcaaactagg aagctagtcg
    12721 caggttgctc aaagcacagc tttgaggttg cagatagagc tgacgtggtt tgaagagatt
    12781 ctcgaatagt ataaaattct tataagaggt ggttgaagtt gttcaactgt tttttgagta
    12841 taaacagtct ttaagaacgt aaaaaagcat aagttcaggt cttcaaaacc ttgttcttat
    12901 gctttttatc atagaaatat ttgctgaatt ttctcttgaa atgaaacctt atcttcatct
    12961 cgcgcttgtc ccttaccctg cttttccgat tgcgagtgcg tagataaaga agatggcgaa
    13021 gccaaagagg aaaatcaatc ccgcaatggc aaggtgtttg agccaagaag gaagattttt
    13081 gttttcacgc gttaccaagg cgtaattcaa aagagcaaag aaaggtgttg tcaggaaaga
    13141 gccaatcatg gcaaagcgga gcatggttga aacctgacca gagaagaact tgataatgac
    13201 gataccgatg atagcagtga tggtcatcca gatgttcaaa gatttacgat tgtcctcttt
    13261 ttgactgatt agcagtcgga gagattcctg attaacgcga gaatagccat cgataacagt
    13321 tataactgtt ccaaagatac agaggaaggc aataaaggta atcaagtaac gggaccattc
    13381 gccaagaaca gaggcataca tgcccacgaa ttgagagatg tatttggctg aagcagcttc
    13441 aaccgcctgc cctgtaggat actgaatcag tgctcccagt gccacaaaga agacggctag
    13501 gatagctgtt ccaatataac cagtgttaaa gtcaaacaga gcgtcttctg tgttaaagtt
    13561 gacggtcttt ctcttttcag ctgaccaaag tgaattgatg gctgaaattt caataggagc
    13621 cggcatccat cctaggaggg agacgatgaa gggcagagct gccatttgcc aaggtgtctt
    13681 ctcgacaaaa tcagaactgt attctggatg cttgaccgcc gcaatgataa ctgccagaac
    13741 agtcgcaatg gttaaggcag acataatcca tttgaccatg ccgtctaaaa gtttgtagcc
    13801 tccaaagagt agcatagccc aaatgattgc aacgagaatg agggaccact gagtaatgct
    13861 aagtccaatc attgggaagg cactggcgat gatagctgag cacagaatgg caacaccagc
    13921 cgtgttgacc atagccgaaa agacattgag gataaagaaa atccagagat agagttttcc
    13981 tttttcggca taaccttcaa ccaaagtctt tccagtatca gctgtgtatt cagcaccaaa
    14041 acggaaaaat ggatatttaa agacattggc taagatgacc aagagaagta gagaccaacc
    14101 gtaagaaccg ccagcttgag ttgaggatac aatgtgggaa cctccaacag cggcagttgc
    14161 cattaggatt ccaggtccca ttgcttttag cttgcttgcc caggttgatt gatttaagga
    14221 aatagcttgc gacatgataa atactccttt ttgaattttc agaataatct gtatatgtaa
    14281 tctatattct acaaaaaatt ttggaaaaat gcaagaatat tttgaaaaaa gatagaaaat
    14341 ttacaaagaa aat
//
LOCUS       DAVBHS010000040        14311 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVBHS010000040 DAVBHS010000000
VERSION     DAVBHS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14311
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.040"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            132..914
                     /gene="thiM"
                     /locus_tag="VQ651_000875"
     CDS             132..914
                     /gene="thiM"
                     /locus_tag="VQ651_000875"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET2753895.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVMIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            916..1545
                     /locus_tag="VQ651_000876"
     CDS             916..1545
                     /locus_tag="VQ651_000876"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET2753896.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      1754..1842
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2104..2664
                     /locus_tag="VQ651_000877"
     CDS             2104..2664
                     /locus_tag="VQ651_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2753897.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGFVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            2665..4050
                     /locus_tag="VQ651_000878"
     CDS             2665..4050
                     /locus_tag="VQ651_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2753898.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIVERVQTVGILLQNPNHQFTMESLFEELVFTMENI
                     GYHLQEIDSKIAEVVQQCRCKDILHRLIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELVYDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGSITWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            4052..4702
                     /locus_tag="VQ651_000879"
     CDS             4052..4702
                     /locus_tag="VQ651_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HET2753899.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAIFPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQKLHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAYGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            4713..5405
                     /gene="tenA"
                     /locus_tag="VQ651_000880"
     CDS             4713..5405
                     /gene="tenA"
                     /locus_tag="VQ651_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HET2753900.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            5410..5934
                     /gene="thiW"
                     /locus_tag="VQ651_000881"
     CDS             5410..5934
                     /gene="thiW"
                     /locus_tag="VQ651_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HET2753901.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERID"
     gene            5934..6737
                     /locus_tag="VQ651_000882"
     CDS             5934..6737
                     /locus_tag="VQ651_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET2753902.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            6730..7362
                     /locus_tag="VQ651_000883"
     CDS             6730..7362
                     /locus_tag="VQ651_000883"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET2753903.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(7571..8362)
                     /gene="thiD"
                     /locus_tag="VQ651_000884"
     CDS             complement(7571..8362)
                     /gene="thiD"
                     /locus_tag="VQ651_000884"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET2753904.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEGMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(8446..8544)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8728..9123
                     /locus_tag="VQ651_000885"
     CDS             8728..9123
                     /locus_tag="VQ651_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HET2753905.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTIQTLL
                     ARLVEKEYLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            9134..9505
                     /locus_tag="VQ651_000886"
     CDS             9134..9505
                     /locus_tag="VQ651_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET2753906.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QVGEFGFACGMNMMHGKMIVE"
     gene            9515..11758
                     /locus_tag="VQ651_000887"
     CDS             9515..11758
                     /locus_tag="VQ651_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET2753907.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYIVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQSLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            11965..13740
                     /gene="spxB"
                     /locus_tag="VQ651_000888"
     CDS             11965..13740
                     /gene="spxB"
                     /locus_tag="VQ651_000888"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HET2753908.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            13851..14198
                     /locus_tag="VQ651_000889"
     CDS             13851..14198
                     /locus_tag="VQ651_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2753909.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
BASE COUNT         4229 a         2611 c         3175 g         4296 t
ORIGIN      
        1 tagcctccta gtttgctctt tgattttcat tgagtataaa tcattcaatg taaatgaaaa
       61 aaattcttaa cgaaagatag aaataaatcg aaatcagtag atcgtataaa gtgaaaagga
      121 aggatttcaa tatgacaagt ttaaaattat taaaagaaaa agcaccattg gtcatttgta
      181 taaccaatga tgtagtaaaa aatttcacag caaatggatt agtagcactg ggcgcctcac
      241 cagccatgag tgagtttcca gcagatttag aggatttgtt aaagtatgct ggtggtttat
      301 taataaacat aggaacattg acagatgaaa attggaaatt ataccaagct gctctgaaaa
      361 ttgcagagaa atataatgtc ccagcagttt tagatcctgt agcctgtgga gcaggagaat
      421 atagaaaaaa agtagcagat gatctaatca acaattataa actagcagcg attagaggaa
      481 atgctggcga gattgcctct ttagtaggaa tagatgtggc atctaaagga gtagatagtg
      541 cgggcgtaga taatattgac gaaattgctc tagcagcaaa tgagaagttc aatattccaa
      601 tagtagtaac aggtgaagtg gatgccattg ctgttaatgg agaagtggta atgattcata
      661 atggtagtgc tatgatgccg aaagtcattg ggacaggatg cttattagga gctgtagtag
      721 caagctttat cggactagaa aaaggtcaag aattgaaatc attagaaaca gcaatgttgg
      781 tttacaatat cgctggagaa atggcagaaa aacgtccaaa tggacatctt cctgggacat
      841 ttaaagttga atttataaat gccttatacg agattacaga tgaagatgta aaggaattca
      901 aaagagtgaa gtaagatgtt tcataaagaa ttactaaaac tatattttat ttgtggaacg
      961 actacttgcc aaggaaaaaa tctatataca gtcgttgagg aagccttaaa aggtggtata
     1021 accttatttc aatttcgaga aaaaggtgag ggtgctttag aaggcttaga aaaacttgag
     1081 ctagctatcc aaattaaaga actttgtaaa aaatacaatg ttccatttat cgttaatgac
     1141 gatatagatt tagcaatgga aattgacgcc gatggcgtac atgtaggtca agatgatatt
     1201 ggtgttgatg aaattagaaa attgatgcca gataaaataa ttggtctttc tataagaaac
     1261 gaagaagaat ttcaacaatc aaaagttgaa tatgtagatt atgtaggtgt tgggcctgta
     1321 tttggtaccc agtcaaagga tgatgctggt ggtactatag gttatgaagg tcttgaattg
     1381 atgagaaaac tattgccaca aatgccttta gttgcaattg gtgggataca gacgaaacat
     1441 attaaagaca ttataaagac caatatggat ggcgtttcaa tcatttcggc aatatcgtat
     1501 gcaaaaaata tagaaaaaac tgttcgggaa atgagtgaac aatagatatc tatccttgga
     1561 ttctaagtaa gtaggatttt ccttttttaa aaagctatgt ccagattcag cttgaatggc
     1621 taattcatca aacaggtcat ttgttttttg caaaagaaag atctgctgtc tttgtgagtg
     1681 caaggctgta tttccagttc aatttcttga actagtataa aatgtgtgtt ataataatca
     1741 cataattaaa taataaagac atttggggtg ctttaggctg agatgatacc cattgaacct
     1801 gatacagtta agactggcga agggaaatgt gaaatgtttt tttcgtatgt cgaaaagaac
     1861 tgtctaatct tgtaagctga actctaattt gtatctgtaa ttagagtttt ttcttttacg
     1921 aaactggatg aagatgatat agtagattga aataagatgc gaacaaatca attagaaaat
     1981 tcaaattaat ttctagaaat attttagtgt tcttgtgtac tgttctaaat tcagtctgct
     2041 atacaaagta tttaggtcgg tcttcttgag tgccccactt ttctttaaaa aaaggagttt
     2101 tttatgttga aaaaatggca gttaaaagat gttatcttgc ttgctttctt gtctatcttt
     2161 tttggtgggg ttttcgttgg ttcaggatat gtgtataata ttctcagtct actcttaaca
     2221 cctcttggtt tgcaggcctt tgccaatgaa atcctcttcg gtctctggtg tatggctgcg
     2281 cccattgctg ccatctttgt tccgagagtc ggaagtgcaa cgattggaga agtgctagct
     2341 gcgcttgctg aagtccttta tggtagccaa tttggtctag gagctctttt gtctggcttt
     2401 gttcaaggtt tgggaagtga atttggtttt atcgtaacta agaatcgcta tgaaagttgg
     2461 ctctctctaa ctgctaatag tattgggatt acgcttgtta gctttgtcta tgaatacatt
     2521 aagttaggtt actacgcctt ttcccttccg tttgtccttt ccttgcttgt ggtacgtttt
     2581 atttctgttt atttcttctg taccatcttg gttcgtgcca ttgtcaaact ctatcatcag
     2641 tttgcaactg gaggaaaagc atagatgggt ctggaactac gagcgattca gtccccaatc
     2701 ttctctgagc cgtttgattt tacttttcat gcgcaagcct ttaccttgtt agttgggagt
     2761 agtgggtctg gaaaatccag cctctttcaa atgattgccc aagttagttc tcttccctat
     2821 agcggtcaag tcctgataga tgggagcgag gtcagtcagc tttctatcgt cgaacgtgtc
     2881 cagacggttg gtattctctt gcaaaatcct aatcatcaat ttaccatgga gagcttgttt
     2941 gaggagttgg tttttaccat ggaaaatatc ggctatcacc ttcaggaaat tgattctaaa
     3001 atagcagagg ttgtccagca atgtcgttgc aaggacatct tgcaccgtct catccatcac
     3061 ttatcaggtg gggaaaagca aaaagcagcg ctggctgtcc tctttgccat gaatcctagg
     3121 gtctatctct tggatgagcc cttcgcttcc attgaccgca agagcagaat cgagatattg
     3181 gagattctaa aagagttggt ctatgatggg aagacagtta ttttgtgcga ccatgattta
     3241 tctgactata aagcctatat cgaccatatg gtggagctaa gagacggaaa actaagggaa
     3301 gtgtttcaaa tcccttccta tgagatgaca caggttgctt caaaggaagt tgcttctagc
     3361 ccggaactat tccatatgaa ccgtgtgact ggtgagcttg gtaatcgccc cctcttttca
     3421 attgctgatt tcacattcta tcaagggatt tcctgtatcc tgggtgacaa tggtgtcggg
     3481 aaatcaaccc tctttcggtc tattcttcaa tttcaaaagt ataaggggag cattacttgg
     3541 aagggttcgg tcctgaaaaa gaaaaagagt ttgtatcgtg atctgactgg tgttgttcag
     3601 gaagctgaga agcagtttat ccgagtcagt ctgcgagagg agcttcaatt agatggacct
     3661 gattctgaaa gaaatcagcg gatttttcaa gctttacgat attttgattt ggagcaggca
     3721 gtcgataaga gtccctatca attaagtggt ggtcagcaaa aaattcttca gctcctgacc
     3781 atcttgacca gtaaggcttc cgtgatcttg ctagatgaac cttttgcagg tttggatgat
     3841 agagcctgcc attatttttg caagtggatt gtggaggaga ggaatcaagg aagaagtttt
     3901 ctgctcatta gtcatcgttt agaccctttg atttctgtgg ttgattattg gattgagatg
     3961 actagtcagg gtcttcggca tgtgaaagaa gtgaccatta ccaaaccact tacatctcag
     4021 agtagcaata cccaagggga ggtgagatag tatggtcaaa gtagcaaccc agacaccgat
     4081 tatcagtctc ttcttgctga ttttatcctt ggaaacatct ttcattcctt cgattgctct
     4141 gaatctttcg gtagtcgcat tttgtattct ctttatgctc tattaccgtc gatttaaaat
     4201 gttagcttgg atgatcatac ttgccatttt cccatctttt gccaactact gggcagttca
     4261 gttacacgga gatgcttcac aggcagtcat gcttggaacg agggcctttg tgacagtttg
     4321 tatcggcctt gtctttgttt cctctatttc actaaaagag cttctcttgt acttggctca
     4381 aaaggggcta tcacgctctt ggtcctatgc cttgattgtg gtattcaatt cttttcctct
     4441 cattcagcaa gaaatcaagt ccctcaaaga agcttgccta ttacgtggtc aaaaactaca
     4501 tttttggtcg cccttgattt acagtaaggt tctgatgaca gtctttaggt ggcgccatct
     4561 ttacctgaga gctctatctg cttacggata tgacgaacat gcacagttga agaatagcta
     4621 tcggactttt tatattccta aaaaaacaaa attaatctac ctgcttttct ttttattgct
     4681 tcaaaccagt ctatttttat aaaggagtta ttatggaatt tacagatatt gcgatggaat
     4741 tatccaagaa agcttggcag gcttcctttc atcatccctt tattttacaa ttacaagagg
     4801 ggaatttaga acctgccatt ttccgctatt acctgattca ggatgcctac tatctgaagg
     4861 ctttctcaga aatctatcat cttttggctg ataagacttc aaaccaagag atgaaaagac
     4921 tcttgaaaca aaatgctcag ggtttagtgg agggtgagtt atttattcgc caacaatttt
     4981 tcaaggaatt ggaaatcaac gaccaggaaa tggagcaaca tccaatcgct ccaacctgtt
     5041 atcattacat ttctcatatt tataggcaat ttgcagaacc gaacttagcc atagcttttg
     5101 caagcttgct tccttgtcct tggttatacc atgatatagg caaatcactt aatcttaaac
     5161 catcaccaaa tcctctctat caacaatgga ttgaaactta tattacggat gagttagagc
     5221 aacagatcag agaggaggga gcactggtca atcagcttta tcgagaaagt gatgagacag
     5281 ataagcaaaa aatgctagat gccttccaca tcagtgttca tatggaagcc aagttttggg
     5341 agatggctta ccaacaccag acatggaaga gcgatttaca gtctttagaa aaaggagaag
     5401 aatagaaaca tgagaaagca ccaactacaa gttcacaaat tgaccatttt atctatgatg
     5461 attgcccttg atgtagtcct tacacctatc tttcggattg agggaatggc accgatgtcc
     5521 agtgtagtca atattctagc gggaatcatg atgggacctg tttatgcctt ggctatggct
     5581 acagtgacag cctttatccg tatgacgact caagggattc cgcctttagc tctcacagga
     5641 gcgacttttg gagcccttct agcaggtctc ttttataagt acggtcgaaa attttacttt
     5701 tctgccttgg gagaaattgt gggaacaggt attattggtt ctattgtttc ctatcctgtt
     5761 atggtgctct ttacaggatc ggctgctaag cttagttggt ttatctacac tcctcgattt
     5821 ttcggagcaa ccttgattgg tacagcgatt tcctttattg catttagatt tttaatcaag
     5881 caggaattct ttaaaaaagt gcaaggatat ttctttgctg aaaggataga ctgatgcagg
     5941 cattgacaaa tccttttcct ataggctcta gttccctcat tcactgtatg accaatgaga
     6001 tttcttgtga gatgctggca aatgggattt tggctctggg atgcaaacct gtcatggcag
     6061 atgatccccg tgaagttctt gattttacta agcaaagtca ggctctcttc atcaatttgg
     6121 ggcatttgtc agctgagaag gaaaaagcaa tccgcatggc agcttcgtat gcaaaccaat
     6181 cttctctccc gatggtagta gatgcggttg gcgtaacgac ttcatccatt cgtaagagct
     6241 tagttaaaga ccttttagac tatagaccta cggtccttaa aggaaacatg tcagaaattc
     6301 gaagtcttgt tggattaaag caccacggcg ttggggtcga tgcgagtgct aaagatcaag
     6361 aaacggagga tttgcttcaa gtcttgaaag actggtgtca gacctatcct ggtatgtctt
     6421 tcttagttac tggccccaag gaccttattg tttcggagaa tcaagttgct gtactggaga
     6481 atggctgtac tgaattagac tggataacag ggacaggaga cttggttgga gccttaacag
     6541 ccgtttttct cagccaagga aagactggtt ttgaagcttc ttgcttagca gtctcttatc
     6601 tcaatatcgc tgctgagaaa atagttgttc aaggaatggg attggaagaa tttcgttacc
     6661 aagtactcaa tcagctttcg ctcctaagaa gagatgaaaa ttggctagat accatcaaag
     6721 gagaggttta tgaatagaga agcacttaga ctatatctgg taaccaatcg ctatcaagat
     6781 tccgtggaaa gctttcttgc aaaagttgag acggcctgcc gttcaggggt taccatagtc
     6841 caattgcgag aaaaaaatct cacaaccaat caatattatc aactggcaaa acaagtcaag
     6901 gaaataacag atgcctatca ggtccccttg ataatcgatg atcggttgga tgtttgtctt
     6961 gcggttgatg cagcaggtct gcatatcgga gatgatgaat taccagtttc ggtagctcgc
     7021 aaagtcttgg gtcctgaaaa aatcctcggt gtcaccgcta aaacggttaa aagagctctg
     7081 gaagcggaga aatcaggtgc agattacttg ggtacaggag ccattttccc aaccaccacc
     7141 aaggaaaatg cgcccattac tctgatatcg accttgaaaa caatttgcca aacggttgcc
     7201 attccagtag ttgctattgg aggcttgact tcggagaata ttgatcagct tatgggcact
     7261 ggcatagctg gtgtagctgt tgtacgtgat ttgatgcaag cagaagatat tgaggcaaaa
     7321 acgcaagcct ttttaacaaa gttgcatgac attctttcct aattaatact caatgaaaat
     7381 caaagagtaa actagaaagc tagtcgcagg ttgctcaaag tactgccttg aggttttaga
     7441 taagactgac gaagtcagct caaaacagtg ttttgaggtt gcagatggaa gctgacgtaa
     7501 tttgaagaga ttttcgaaga gtataaaaac tctctaattc ccttaaagtg ggaactagag
     7561 agtttttttc ttaatcttta aaagttgtat ggttgactgg accagaacca tgaccgagtt
     7621 gaggggcatc ttgaatagct tttgtgataa aggccttggc cttatcaact gcctggtaaa
     7681 gactcttgcc cttggctagt tcagcagtaa tcactgcagc aaaggtacat ccagtaccat
     7741 gggtgtgaca ggtttgaatt cgtgggcttt cccagacaaa ttgttcattc ttggtaaaga
     7801 ggaaatcttt agcaccacct ttgagatgtc cgcctttgat aaccacagac tgaggaccaa
     7861 attcttttaa aatcaggcga ccagcacgct gcatgccttc ggggtcatgg attgaaaaac
     7921 caacaatctc ttctgcttca ggaagatttg gcgtaataat agttgctaga ggtagtaagt
     7981 ttgttttgag atagtctcta gcacttgagt caatcaaggc atctccactt gtagcaacca
     8041 taacaggatc aaggacatag ggacaatcca gttttttaag atagggttgg atgatttcca
     8101 tgatttcagt agtagccaac attccagttt ttacagcctg aggtggaata tcagaaaaga
     8161 cactctccaa ttgggctttc aacatttgag gagaaacgtg ctcgattagc tgaacacctc
     8221 tggtattttg agcgacaaga ctggttacaa cagccattcc atagacatct ctcgcttgga
     8281 atgactttaa atctgccata atgccagcac caccactagg gtcagtccct gcaatggtca
     8341 aagcaacggg taaataagtc atctaaaacc tcccaaccaa ctgaagtttg tattcttaag
     8401 aacaagctag tctgttttag aaaggcaata gaagactgct agtccccatt atcatttcca
     8461 cttccatcag ctagcattac ctagattgag tcagagggat ctaacagtcg ttaatctcag
     8521 ctttacgcac ccctagtggt tgttttcttt tttctttagt atagcatatt tttattgttt
     8581 atatagtaat atttgactgc aaatcgtttt tcaaatgatt aaaattctat ttttcgaaga
     8641 aaatattatt tctataattg acaaatgtag attttaagag tatactgatg agtgtaattg
     8701 acaaatgtag attttggagg tatggtaatg cagatttcag atgcagaatg gcaggtcatg
     8761 aagattattt ggatgcaggg ggagcagacc agtacagatt tgattagggt tttggcagag
     8821 cggttcgact ggtccaagtc gaccattcaa actcttttgg ctcgtttggt tgagaaagag
     8881 tatctgactc ggaaaaaaga aggcaagtcc tttgtttatt cagccctttt aactctagac
     8941 caaagtcggg atttacttgt ccaagatatc aaagacaagg tttgttcccg taggattagg
     9001 aatttgttgg ctgacttgat tgtagaatgt gaatttactc agactgactt ggaagacttg
     9061 gaagctgtga tttcagagaa gaaatcaagt gctgtaacag aagtaagatg taattgtatg
     9121 taaaggagac gtcatgttaa atagtattgt aaccattatt tgtattgccc ttatcgcgtt
     9181 tatcttgttt tggtttttca aaaagcctga aaaatctgga caaaaagccc agcaaaaaaa
     9241 cggataccaa gagattcgag tggaagtcat gggaggctat actcctgagt tgattgtcct
     9301 caagaaatca gtgccagcac gcattgtctt tgaccgcaag gatccttcac catgtctgga
     9361 tcaaattgtt tttccagatt ttggtgtaca tgcgaacctg ccaatggggg aagagtatgt
     9421 agtggaaatc acgcctgagc aggttggaga gtttggcttt gcttgtggta tgaacatgat
     9481 gcacggcaag atgattgtag agtaggtgga gactatgaca gaaattgtga aagcaagctt
     9541 agaaaatggc attcaaaaaa tccgtatccg agctgaaaaa ggctatcatc cagcccatat
     9601 ccagcttcaa aagggaattc cagctgagat tacctttcat cgtgctactc cttcaaactg
     9661 ttataaggaa attctgtttg aagaagaagg tatcttggaa ccaatcggcg tagatgagga
     9721 gaaagtcatt cgttttacac ctcaagaatt agggcgacat gaattttctt gtggcatgaa
     9781 gatgcaaaag ggaagctata tagtcgttga gaagactcga aaatctctat ctctcctgca
     9841 acgtttttgg attactagta tctttactgt gcctcttgtg attctcatga ttgggatgtt
     9901 gacaggtagc attagtcatc aagtcatgca ttggggaacc tttctagcaa caacgcctat
     9961 tatgttagtt gcaggtaagc catatatcca gagtgcttgg gccagtttta aaaagcacaa
    10021 tgccaacatg gataccttgg ttgcgctggg aactctagtg gcttatttct atagcctagt
    10081 tgctctcttt gctggtctcc ctgtttactt cgaaagtgct ggatttatcc tctttttcgt
    10141 tcttttggga gcagtttttg aggaaaaaat gagaaaaaat acgtcccaag ctgtggagaa
    10201 attactggac ttgcaagcta aaaccgcaga agtcttgagt gatgatagtt atgtccaagt
    10261 tcctttggaa caagtcaagg taggcgacct gattcgagtg cgtcccggtg aaaagattgc
    10321 tgttgatggt gtcgtagtag aaggtgtctc tagtattgac gaatccatgg tgacaggtga
    10381 gagtctgcct gtggacaaga cagttggaga tactgtcatt ggctcaacca tcaatcatag
    10441 tggaacgctt gtctttagag cagaaaaagt tggctcagag actgttttgg ctcagattgt
    10501 ggattttgtg aagaaagctc agacaagtcg tgcgccgatt caggacttga cggataagat
    10561 ttcagggatt tttgtcccag tagttgtcat tttaggaatc atgacctttt gggtttggtt
    10621 cgtcttgctc agggatagtg tggtcgtgct tggagctagc tttgtgtcct ctcttctcta
    10681 cggagtggcg gttttgatta tcgcctgtcc ttgtgccttg ggacttgcaa caccgacagc
    10741 ccttatggtg gggacaggac gtagtgccaa gatgggggtt ctcctcaaaa atggaactgt
    10801 cttacaggaa atccagaaag ttcaaactct tgtctttgat aagaccggga ctttgacgga
    10861 agggaaacct gtggtaacag atatcatcgg cgacgaagta gaagtgtttg gattggcagc
    10921 ctccttggaa gatgcttctc aacacccact ggctgaggct atcgttaagc gagcgagtga
    10981 agctggactt gagtttcaaa ctgttgaaaa ttttcagtcc ttgcacggga aaggtgtttc
    11041 agggcgaatc aatggaaaac aagttttact tggaaatgct aaaatgctgg atggtatgga
    11101 tatttctaat acttatcaag ataaactaga agaactagaa aaagaagcta agacagttgt
    11161 gttcttagct gttgacaatg aaatcaaagg cttgcttgct ttgcaagata ttcctaagga
    11221 aaatgctaag ctagccatca gtcagctaaa aaaacgtggt ctccgaacag tcatgctgac
    11281 aggagacaat gctggtgtgg cgcgtgctat tgcagatcaa atcggaattg aagaggtcat
    11341 tgcaggcgtc ttgccagaag aaaaagccca tgaaatccat aaactgcaac agtcagggaa
    11401 agtagccttt gttggggacg gtatcaatga cgctcctgcc cttagtgtag cagatgtggg
    11461 gattgctatg ggagctggaa cagatatcgc catcgagtca gcagatttgg tgttgacaac
    11521 caataatctt ttaggagtgg ttcgtgcctt tgatatgagt aagaaaacct ttcatcgaat
    11581 tctactcaat cttttctggg cttttatcta caatgttgtc ggaattccga ttgcagcagg
    11641 agtcttttca ggtgttggac tggctctcaa cccagaattg gcaggtctag ccatggcctt
    11701 tagttctgta tctgttctga ccagttccct tctgttaaat tttagtaaaa tagactaaaa
    11761 gtaatgacta gatttctttg ttataaaaca gaaatgacag aactttcaaa actgtattag
    11821 aatagagaat agagagtttt gagcagattt ttagaaaagt cagcataaat atgatacagt
    11881 ggaatagtaa aaatttggag aacgtttcca attctatgta atcgtattct ccaaatctaa
    11941 aaaaattgaa ggagagttat cattatgact caagggaaaa ttactgcatc tgcagcaatg
    12001 cttaacgtat tgaaaacatg gggcgtagat acaatctacg gtatcccatc aggaacactc
    12061 agctcattga tggacgcttt ggctgaagac aaagatatcc gcttcttaca agttcgccac
    12121 gaagagacag gtgctcttgc agcggttatg caagctaaat tcggcggctc aatcggggtt
    12181 gcagttggtt caggtggtcc aggtgcgact cacttgatta acggtgttta cgatgcagct
    12241 atggataaca ctccattcct agcgatcctt ggatcacgtc cagttaacga attgaacatg
    12301 gatgctttcc aagagcttaa ccaaaaccca atgtacaacg gtatcgctgt ttacaacaaa
    12361 cgtgtagctt acgctgagca attgccaaaa gtaattgacg aagcctgccg tgctgcaatt
    12421 tctaaaaaag gtccagctgt tgttgaaatt ccagtaaact tcggtttcca agaaatcgac
    12481 gaaaactcat actacggttc aggttcatac gaacgctcat tcatcgctcc tgctttgaac
    12541 gaagttgaaa tcgacaaagc tgttgaaatc ttgaacaatg ctgaacgccc agttatctat
    12601 gctggatttg gtggtgttaa agctggtgaa gtgattactg aattgtcacg taaaatcaaa
    12661 gcaccaatca tcacaactgg taaaaacttt gaagctttcg aatggaacta tgaaggtttg
    12721 acaggttctg cttaccgtgt tggttggaaa ccagccaacg aagtggtctt tgaagcagac
    12781 acagttcttt tccttggttc aaacttccca tttgctgaag tttacgaagc attcaagaac
    12841 actgaaaaat tcatccaagt cgatatcgac ccttacaaac ttggtaaacg tcatgccctt
    12901 gacgcttcaa tccttggtga tgctggtcaa gcagctaaag ctatccttga caaagtaaac
    12961 ccagttgaat caactccatg gtggcgtgca aacgttaaga acaaccaaaa ctggcgtgat
    13021 tacatgaaca aactcgaagg taaaactgag ggtgaattgc aattgtatca agtttacaat
    13081 gcaatcaaca aacatgctga tcaagacgct atctactcaa tcgacgtagg taacactact
    13141 caaacatcta ctcgtcacct tcacatgaca cctaagaata tgtggcgtac atctccgctc
    13201 tttgcgacaa tgggtattgc ccttcctggt ggtatcgctg ctaagaaaga caatccagat
    13261 cgccaagtat ggaacatcat gggtgatgga gcattcaaca tgtgctaccc agacgttatc
    13321 acaaacgttc aatacgacct tccagttatc aaccttgtct tctcaaatgc tgagtacggc
    13381 ttcatcaaga acaaatacga agatacaaac aaacacttgt ttggtgtaga cttcacaaac
    13441 gctgactacg ctaaaattgc ggaagctcaa ggagctgttg gattcacagt tgaccgtatc
    13501 gaagacatcg atgcagttgt tgcagaagct gttaaattga acaaagaagg taaaactgtt
    13561 gtcatcgatg ctcgcatcac tcaacaccgt ccacttccag tagaagtact tgaattggat
    13621 ccaaaacttc actcagaaga agctatcaaa gccttcaagg aaaaatacga agcagaagaa
    13681 ctcgtaccat tccgtctctt cttggaagaa gaaggattgc aatcacgcgc aattaaataa
    13741 ttcctctcgc cgaaaatcaa atatgaaact tgtagacttt attctttgat tttcatagag
    13801 cagaacttta aaagatcgga gcaattcggt ctttttatgg aggagagtaa atgaatttaa
    13861 atcaattaga tattatcgtt tcaaatgttc cccaagtctg tgctgacttg gagcatattt
    13921 tggataaaaa ggcagattat gctgatgatg gttttgctca gtttacgatt ggtagtcact
    13981 gccttatgtt gtcacaaaat catttggttc ctttggaaaa ctttcagtca ggaatcatta
    14041 ttcatatcga ggttgaggat gtagaccaga actacaaacg gttgaatgag cttggtataa
    14101 aggttttaca cggaccaact gtaaccgatt ggggaacaga gtccttatta gttcaaggac
    14161 ctgctggtct agtgcttgat ttttatcgta tgaaataggt cgcgtagtca tttgacgtta
    14221 accttagcta tttttaaaat agaaattgag aatatgaact attgaaagat cggagcaatc
    14281 cggtcttttt atataaagaa attgcatata a
//
LOCUS       DAVBHS010000041        14021 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVBHS010000041 DAVBHS010000000
VERSION     DAVBHS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14021
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.041"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(144..758)
                     /locus_tag="VQ651_000890"
     CDS             complement(144..758)
                     /locus_tag="VQ651_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HET2753910.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1574..1843)
                     /gene="rpsO"
                     /locus_tag="VQ651_000891"
     CDS             complement(1574..1843)
                     /gene="rpsO"
                     /locus_tag="VQ651_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET2753911.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(2069..2614)
                     /locus_tag="VQ651_000892"
     CDS             complement(2069..2614)
                     /locus_tag="VQ651_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET2753912.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGSGS
                     LYIDETKTTRLLDTPDFPEEFKSLAKAQADELDLLRTKNNLNWAFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKRNKPD"
     gene            2853..3053
                     /locus_tag="VQ651_000893"
     CDS             2853..3053
                     /locus_tag="VQ651_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753913.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKGLYINNLL
                     FFTPMVILIVSLLYSYRFRFSL"
     gene            3484..3624
                     /locus_tag="VQ651_000894"
     CDS             3484..3624
                     /locus_tag="VQ651_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HET2753914.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            3631..3807
                     /locus_tag="VQ651_000895"
                     /pseudo
     CDS             3631..3807
                     /locus_tag="VQ651_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
     gene            3865..5808
                     /gene="thrS"
                     /locus_tag="VQ651_000896"
     CDS             3865..5808
                     /gene="thrS"
                     /locus_tag="VQ651_000896"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET2753915.1"
                     /translation="MINITFPDGAIREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHGDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(5857..6831)
                     /locus_tag="VQ651_000897"
     CDS             complement(5857..6831)
                     /locus_tag="VQ651_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET2753916.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVEMQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6824..7501)
                     /locus_tag="VQ651_000898"
     CDS             complement(6824..7501)
                     /locus_tag="VQ651_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2753917.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            7657..8730
                     /locus_tag="VQ651_000899"
     CDS             7657..8730
                     /locus_tag="VQ651_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HET2753918.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDKLAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHKIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(8821..9123)
                     /locus_tag="VQ651_000900"
     CDS             complement(8821..9123)
                     /locus_tag="VQ651_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753919.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(9191..9628)
                     /locus_tag="VQ651_000901"
     CDS             complement(9191..9628)
                     /locus_tag="VQ651_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET2753920.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKKSKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTDIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(9704..10546)
                     /locus_tag="VQ651_000902"
     CDS             complement(9704..10546)
                     /locus_tag="VQ651_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET2753921.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            10658..11308
                     /locus_tag="VQ651_000903"
     CDS             10658..11308
                     /locus_tag="VQ651_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET2753922.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRMHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            11519..12153
                     /locus_tag="VQ651_000904"
                     /pseudo
     CDS             11519..12153
                     /locus_tag="VQ651_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
     gene            12284..12721
                     /locus_tag="VQ651_000905"
     CDS             12284..12721
                     /locus_tag="VQ651_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753923.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISNTYTILSISSFLV"
     gene            complement(12816..13115)
                     /locus_tag="VQ651_000906"
     CDS             complement(12816..13115)
                     /locus_tag="VQ651_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753924.1"
                     /translation="MNRNVLNFLRTESAERVSLYIDKANRLEGDVTLLAPSSQDLEDI
                     KNAMFSNPNLELKVARLDVMKKIAYASTRNHYLTGATIFGDISKGTYNCDPKSYV"
     gene            complement(13523..13885)
                     /locus_tag="VQ651_000907"
     CDS             complement(13523..13885)
                     /locus_tag="VQ651_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753925.1"
                     /translation="MRLASESRQILDKLKDDVYNKLGYEVSYSSIVSQAVREYVPKKE
                     RIDWIKLKETAIPFSSLKQSNNWEYQTSLMLEKDVLILLSELQNFFLDVFQAKRIHRA
                     FCIRLCLRAQFLLSNNDS"
BASE COUNT         4303 a         3062 c         2450 g         4206 t
ORIGIN      
        1 ttataagaat ctcctaatca aatcaaaagc cactctacaa tagagtaact ttaagattgt
       61 ccatttatac tcaatgaaaa agccctctga aaatcagaga gctgatttga gctcggacta
      121 aaatcctagt gaaacaaaaa aatctacacg atcagaaaag tctctatcgt gccattttca
      181 tacatgatgt atagtccaag tagaatgaat actaagggca caatgattcg ctcgtatttt
      241 tcaattgtct cgaatattaa cggaatagaa gataacaccc gactaatctc gcaaaagata
      301 attatgccga ttacaaacac aagcaaggcc acgagggtct gtgaccaatc taacgaagca
      361 aaataaggta tatagatacc taaattatct ccgccagacg caattgtcag caatgtaact
      421 gtccaaaaca gttgatttgc cttgctttgt tctaatcttt caataatttc ttcctcttct
      481 tcttccgcat cttctccaac aattgcaaag cgaatcccta aatagatagg gattaaacca
      541 agcaatccaa ccatccattc ttcaggcacg aaattaacga cataagcagc aactaaactc
      601 gcccctacaa gtaagcctgt gcctagatat tgccccgcat aaatatgcca tttctgttta
      661 ttctgtgata gctgtgcaaa taaaataatt aaaataatta aataatcgat actggtggaa
      721 atataaacac caatagcaga tatgattgtc tgtcccataa aaaaaaactc ctgtattagt
      781 cagtaataaa tcagcagatt ttaggagagc acagacacat taatttagct atcgctagtg
      841 ggtaatatcg aacgtaggaa agcattttac tcctcctcaa aacgtagtta caaagtaatt
      901 tctaactgga attcagtgac tacttccatg taaagtataa catactatac tttactttgt
      961 aaagagtggg ctcttctagg gggcttgcat acaactactc ggcttgaaaa gccgagtaac
     1021 tgcgcacctt tatggtgtaa ttagctgata ccgtttgagg tttttgtagt ctccaaaatt
     1081 gttaccgata aaacctcaat aaaaaagccc tctgaagtca gagagctgat ttgagtccgg
     1141 gctaaaatcc tagtgaaaaa gatgaaactc cttgtgctca tcgaacactg tgtcatttcc
     1201 ctattttcat acggattttt gacgccctta gcatcatgat tcttgctaga taaatcgttt
     1261 atagactagg cgacgaggcg aagatcgtac gaaactttca gtgaaacaaa aaaaatctcc
     1321 ccgaagggag aaaagctggt ttattttacc ttacagtgct aggaaccgta accaaagatt
     1381 atacttgagt atatcgaggt aaggtgacaa cacaaaaaag ctacccaatt tagagtagct
     1441 tcattatcaa gatatgctca attgaacacg gcctaagcac ttggcaaaaa agataaaatc
     1501 tcctagaaac tgaagtttct tcgtaaattt tcctattttt actttgtgct tttgacagcc
     1561 tttgtatctt gaattagcga cgaagtccta gagagttgat caactcacgg taacggttaa
     1621 cgtcgttttt acgcaagtat gcaagcaagt tacgacggcg accgattttt ttcatcaatc
     1681 cacggtaagt agcgtggtct tttttgtgtt gtttgatgtg ttcattaagg tggttgattt
     1741 cccaagtaag gacagcaact tgaacctcta ctgaacctgt atcaccttcg tgacgtgcat
     1801 attgtgcgat gatttcattt tttttctctt ttgagattgc catgatgttt ctccttttta
     1861 tttggcttca tccgagtgac aggttggcat gcctgtaacc aagaagaagt tatttgtctt
     1921 tacgacaatc cctattctac caagatttaa attttctgtc aaacattaat actctcatag
     1981 ttattatttt tcaagtaaag agatacgttc ttttatatga tgaccttttt caagttcatc
     2041 taccaatcca atagcataat ctgcataact aatctggctt attccttttt tcattagtag
     2101 taaaaatttc tcctgctaga atgtagtttc ctgttttttc accatcggga ataaaatcta
     2161 cagcaggact gacaaaagcc cagttgagat tattttttgt acgaagaaga tccaattcat
     2221 ccgcttgagc ttttgctagt gacttaaatt cttcaggaaa atcaggcgta tctaaaagtc
     2281 gtgtcgtttt tgtttcatct atataaaggc ttcctgatcc ccctacaact agaaaacgtg
     2341 tctgtgttcc agcaagaagc tgattaaata gttcgattga tttgctgtgg agcggtagcg
     2401 tatctggtgt ataagcacca aacgctgaaa taacagcatc aaatccagta agatcatctt
     2461 ttgtcaactc aaataaatct tttttaataa tagactcagc ttgacttttg ttttcagaac
     2521 gaacaatagc cgttacttca tgtcctcgtt tgactgcttc ttcaacaatt gccttccccg
     2581 cttgtccatt tgctgcaata actgctagtt tcatttttta tacccctctt gttgtaatta
     2641 ttttagttac aacaattgtg acactcttaa taatcaatgt caatagtctt gcttaattat
     2701 tatcaaaata tttctaccaa gaaaactaac catgattcta gtgaaaagaa aaatcttctt
     2761 tgtcaacaaa tttactttct tgttttaaac atactataat aatcatagca agagatctaa
     2821 gttgtctgtt tttttaaaac gaggtgatta tcatgcgtag attctattcc catctcccct
     2881 actatctggt catattattc ttttattggc cactttatga gttgttcttg ctagttgttt
     2941 ctgaccccct tacactcaag ggactctata taaacaatct tctcttcttt acacctatgg
     3001 taatcttgat tgtatcgtta ctctatagct accgtttccg tttctcactt tgatggttag
     3061 ttggtaacgg actgctcttt tactttacta tcataacctt tggtgtgttt atactaattt
     3121 acttgctaat ctatgaaaca gttgctctgg tgggcatgga ttctggtatt agcatcaagc
     3181 atattctaca aaaaatgaaa aacaaaaaac tttcacaaaa tccttgaaaa atctcacaat
     3241 catgctataa taatccatag agacaagtca cttagtccct ttctactaga gagtgcgtgg
     3301 ttgctggaaa cgcataggaa gtctaaactg atactactct tgagtttttt atgaaaacat
     3361 aaaacggtgg ccacgttaga gccgatcaga ggtgtccctc tcttttgagg tacataaatg
     3421 aaggtggaac cacgttgcga cgtcctttcg aggatgtcgc atttttttat taggatacta
     3481 attatggagt tgcaagaatt agtggagcgc agttgggcaa tccgacaagc ttatcacgaa
     3541 ctggaagtta agcatcatga tttcaagtgg acggtagaag aagacctctt ggctttatct
     3601 aatgatattg gaaatttcca atgactggtg atgacaaagc aaggacgcta ctatgatgaa
     3661 acaccctaca cactggaaca aaaactttca gaaaatatct ggtgactatt agaactttct
     3721 caacgtttgg atatagatat tctgacggaa atggaaaact tcctctctga taaagaaaag
     3781 caattgaacg ttagaacttg gaagtagtct gctgataaaa aatcaatgct tagaaactat
     3841 gaaataataa aaaaggagaa catcatgatt aacattactt tcccagatgg cgctattcgt
     3901 gaattcgaat ctggcgtaac aacttttgaa attgcccaat ctatcagcaa ttccctagct
     3961 aaaaaagcct tggctggtaa attcaacggc aaactcatcg acactactcg cgctatcact
     4021 gaagatggaa gcatcgaaat tgtgacacct gatcacggag atgcccttcc aatcttgcgt
     4081 cactcagcag ctcacttgtt cgcccaagca gctcgtcgtc ttttcccaga cattcacttg
     4141 ggagttggtc cagccatcga agatggtttc tactacgata ctgacaacac agctggtcaa
     4201 atctctaacg aagaccttcc tcgtatcgaa gaagaaatgc aaaaaatcgt caaagaaaac
     4261 ttcccatcta ttcgtgaaga agtgactaaa gacgaggcac gtgaaatctt caaaaatgac
     4321 ccttacaagt tggaattgat tgaagaacac tcagaagacg aaggcggttt gactatctat
     4381 cgtcagggtg aatatgtaga cctctgccgt ggacctcacg ttccatcaac aggtcgtatc
     4441 caaatcttcc accttctcca tgtagctggt gcgtactggc gtggaaacag cgacaacgct
     4501 atgatgcaac gtatctacgg tacagcttgg tttgacaaga aagacttgaa aaactacctt
     4561 caaatgcgtg aagaagctaa ggaacgtgac caccgtaaac ttggtaaaga gcttgacctc
     4621 tttatgattt cacaagaagt gggacaaggt ttgccattct ggttgccaaa tggtgcgact
     4681 atccgtcgtg aattggaacg ctacatcgta aacaaagagt tggcttctgg ctaccaacac
     4741 gtctacactc caccacttgc ttctgttgag ctttacaaga cttctggtca ctgggatcat
     4801 taccaagaag acatgttccc aaccatggac atgggtgacg gggaagaatt tgtccttcgt
     4861 ccaatgaact gtccgcacca catccaagtt ttcaaacacc atgttcactc ttaccgtgaa
     4921 ttgccaatcc gtatcgctga aatcggtatg atgcaccgtt acgaaaaatc tggtgccctc
     4981 actggccttc aacgtgtacg tgaaatgtca ctcaacgacg gtcacctatt cgttactcca
     5041 gaacaaatcc aagaagaatt ccaacgtgcc cttcagttga ttatcgatgt ttatgaagac
     5101 ttcaacttga ctgactaccg cttccgcctc tctcttcgtg accctcaaga tactcataag
     5161 tactttgata acgatgagat gtgggaaaat gcccaaacca tgcttcgtgc agctcttgat
     5221 gaaatgggcg tggactactt tgaagccgaa ggtgaagcag ccttctacgg accaaaattg
     5281 gatatccaga ttaaaactgc ccttggaaaa gaagaaaccc tttctactat ccaacttgat
     5341 ttcttgttgc cagaacgctt cgacctcaaa tacatcggag ctgatggcga agatcaccgt
     5401 ccagtcatga tccaccgtgg ggttatctca actatggaac gcttcacagc tatcttgatt
     5461 gagaactaca agggggcctt cccaacatgg ctggcaccac accaagtaac cctcatccca
     5521 gtatctaacg aaaaacacgt ggactacgct tgggaagtag ccaagaaact ccgtgaccgc
     5581 ggtgtccgtg cagacgtaga tgagcgcaat gaaaaaatgc agttcaagat ccgtgcttca
     5641 caaaccagca agattcctta ccaattaatt gttggagaca aagaaatgga agacgaaaca
     5701 gtcaacgttc gtcgctacgg ccaaaaagaa acacaaactg tctcagttga taattttgtt
     5761 caagctatcc tagctgatat cgccaacaaa tcacgcgttg agaaataaga gtctagcata
     5821 aaagcctcca atctggaggc tttttctcat ctatttttac tcaaggacta agttcacttg
     5881 agcaaactga atccgcactg tcgttccttt tccgacctca gactcgatac gaatctggtg
     5941 ccccagttct tcagaaattt tcttagatag ataaaggcca agtccagagg actgctgggt
     6001 caaacggcca ttgtatcctg aaaagccacg ttcaaatact cggaggacat cactgttttt
     6061 tatcccgatt cccgtatctt tgatacaaag ctcttggtca tccatataaa tctccagacc
     6121 accttccttg gtgtacttga gactgtttga gatgatttgc tcaataacca ctagcagcca
     6181 ctttttatcc gtcacgattt ctttatcaag gtcatgtaga ttgacattta agcctttttg
     6241 aataaagaaa agagcatatt tacgaattat ttccttgacc aagtcctcaa tttgaacctg
     6301 ctttaagagc aaatcatcat ggaaactttc taaacgcagg tactgtaaaa ctaggttggt
     6361 ataagagtcg attttgaaaa tttcctgttc tagctgctgc ttcagttggc ggtcgaccac
     6421 ttctgcaact aagagttgac tggctgcaat gggggtcttt atctgatgga cccacaaggt
     6481 atagtaatcc agcaaatccg tcagttttct ttctgctttt gacctctgct gatagagttc
     6541 catctcacgc gcttctaatt tttctgctaa agctatttcc aaaggagact tggcttccct
     6601 ctctccatag agaagttcct ggcgatagac ctgcatttcc accaatatgt cccaagtgaa
     6661 aaataagatg gttacaaagc aacacaagaa gaaaaagtag aggaagtaaa ttcctagact
     6721 ggcaaataaa aactgaaaga gtaagacaag aaatgccaaa gaaagcagat agataaaaag
     6781 acgactacgg gagcgcagat aggctagaaa aaattgtttc caatcaagca tgcttcaatc
     6841 cgtaccctat tcctttcttg gtctcgataa atcctaccaa tccctgctcc tccaactttt
     6901 tacgcaaacg agccacattg acagagaggg tattatcatc aatgaaaaag tcactgttcc
     6961 aaagttcccg catcaggtcg tcacgtgcta cgatgttgcc tgcatgctca aataacacgc
     7021 gtaaaatctg gaattcattc ttggtcaaat tcaagacttg cccttgataa tgtaaatcca
     7081 tggatttggt attgaggata acaccagcat attccagcaa actctcatca cgcccaaact
     7141 cataggaacg acgcaacaag ccctgaacct tagctaaaag aacctgctgg tcaaaaggct
     7201 tggtcacaaa gtcatccgcc cccatattga ttgccatgac aatatccata gcctggtctc
     7261 tcgaagaaag aaacatgata ggtaccttgg aaatcttgcg gatttcctga caccagtgat
     7321 aaccattaaa caagggcaaa ccaatatcca tgaggaccag atgaggttcc gactgaacaa
     7381 atagactcaa aacttccata aagtcttcta ccaggaccac ttcaaatccc cattcagaga
     7441 gcattttccc aatctgttga cgaatgacct gatcatcttc tattaataaa atcttgtgca
     7501 tgcgcttctc cttttccatt attataacag atttttccat gctagatggt ctgaaactga
     7561 atttgaaata gcctgttttt agccagtgca aacaggctat gctactagct aatttgaggg
     7621 aaatttgcta agataaataa aaagaaagga gctcttatgg ccaatatttt tgactatctg
     7681 aaagatgtcg catatgattc ttattacgac cttcccttga atgagttaga cattctaacc
     7741 ttaatagaaa tcacctacct ctcctttgat aatctggtct ccacacttcc tcaacgtctt
     7801 ttagatctag cacctcaggt tccaagagag cccaccatgc ttactagcaa aaatcgcctt
     7861 caattattag ataaattggc tcaacacaag cgcttcaaaa attgcaaact ctcccatttt
     7921 atcaacgaca tcgaccctga actgcaaaag caatttgcgg ctatgactta tcgtgtcagc
     7981 ctcgatacct atctgattgt ctttcgtggg acagatgaca gtatcattgg ctggaaggaa
     8041 gatttccacc tgacctatat gaaggaaatt cctgctcaaa agcacgccct tcgctattta
     8101 aagaactttt ttgcccatca tcctaagcaa aaggttattc tagctgggca ttccaaggga
     8161 ggaaatctcg ctatctatgc tgctagccaa attgagcaaa gtttgcaaaa tcagatcaca
     8221 gcagtttata catttgatgc acctggtctc catcaagaat tgacacagac tgcgggttat
     8281 caaaggataa tggatagaag caagatattc attccacaag gttccattat cggtatgatg
     8341 ctggaaattc ctgctcacaa aatcatcgtt cagagtactg ccctgggtgg catcgcccag
     8401 cacgatacct ttagttggca gattgaggac aagcacttcg tccaactgga taagaccaac
     8461 agtgatagcc agcaagtaga cacaaccttt aaagaatggg tggccacagt ccctgacgaa
     8521 gaacttcagc tctacttcga cctcttcttt ggcactattc ttgaggctgg tattagctct
     8581 atcaatgact tggcttcctt aaaggcgctt gaatacattc atcatctctt tgtccaagct
     8641 caatccctca ctccagaaga aagagaaacc ttgggtcgcc ttacccagtt attgattgat
     8701 actcgttacc aggcatggaa aaatagataa tactcttgaa aattaaatgt atacaaaaca
     8761 aaagacctag aatacatact ttcatgtgca ttctaagtct ttttaaatag aatctaatag
     8821 tcaataaaaa tcaaagagca ttgagagata atggggcttg gaacgtccct ctcgcttcaa
     8881 caaaatgacc ccattataga ttaaaaagat gccacttaga aaaagcaaaa aaggaagtaa
     8941 gacaaaggca aatacataaa aagctaactg aacattctcg tatccatttt tataaaaaag
     9001 gtaggataga taaaaataac ttgaaatgag ggataataaa aataatactg gattccacaa
     9061 acttctatta tccttccaaa atgacactat aaaggctaat acaattccta taacgagata
     9121 catttcttac tcctttaata gctacatttt atcataatta tccaaagaaa aaagagggca
     9181 tttatccctc ttaatccttc atctgactct ctgcatcggc cacgactttt tctagactgg
     9241 tttgaccaag ttctgcctcc atagtcaact gaattctctc caatttttga tccaaaacat
     9301 catgaatatg agctcctaca gggcaatttg gattcggatt gtcatggaaa ctgaagagtt
     9361 gacctgtctt accaagacat tcgaccgcct gataaacatc taaaagacta atatccttaa
     9421 ggtccttgac aatatctgtt ccgcccgttc cacgcgctac tgaaatcagc tctgccttct
     9481 tcaactggga caagatcttt ctgataatga caggattgac cccgacacta gcagccagaa
     9541 aatcactggt caccttgctt ttcttcccct cgagggcaat gattatcagc atatgagtcg
     9601 caatggtaaa tctacttgga atttgcatcc tcttctcctt tttacgaggc taccctgcct
     9661 ctactcttct ttttctatta ttataccctt tttagttgta atgtcaatcg ttaccacttt
     9721 tcaaccagtc gtctaactcc cgatcgcaac cctctttctg agccaattct ctcaaaaatt
     9781 cctgatgatg agtatggtgg atcccattga ccagactttc atagtaaacc tcaaaatagg
     9841 gaagtctcag gtctttagcc agctgcaatt cagctgccac atcgtaatct acccgtcgga
     9901 agtccatatc taccaggccc ttgtcatcaa actccaaaat catatactgg gcccgcaagt
     9961 ccttccgtag ctgagcgtcc aaaaagaaag gttggccaat cgaacccgga ttgacaatca
    10021 attgcccacc agtcccgtaa cgaagcaact gctggtgaat gtgtccataa acagcaatat
    10081 cacagggagg atgagtcacc aagcggtcaa attcctcttg tttgccagta tgaatcaact
    10141 ctcgccccca gttcttatca ggcagatgat ggctaattcc cactgtcaaa tccccaaact
    10201 gacgatgaat ttgaagaggt tgattgtgga gcacttcaat ttcttctagg gaaatttcct
    10261 ctaaaacata ctggcactgg cgcaagagat agcgttgact agggcgagta ctatccaatt
    10321 ccttacggac accatgccaa agactgtctt cccagtttcc caaaactcta gccgtaatcg
    10381 gtagttgatc caacaagtcc aaaatccttc tacgccctgt ccctggcatg agaatatctc
    10441 ccaaaagcca gtattcatcc actcctagct gccgagcatc tgccaaaaca gcctccaagg
    10501 cggtggtatt tccatgaata tctgaaagaa gagctatttt cgtcatatcc atctcctcgt
    10561 tttttctctt gcaataagta taacataaaa agtcacagct agagaaatct agcttttttt
    10621 gatatactag ataaagatat tagacaagag gaaacgaatg accccaaaca aagaagacta
    10681 tctaaaatgt atttatgaaa ttggcataga cctgcataag attaccaaca aggaaattgc
    10741 ggctcgcatg caagtctctc cccctgccgt aactgaaatg atcaaacgaa tgaaaagtga
    10801 aaatctcatc ctaaaggaca aggaatgtgg ctatctactg actgacctcg gtctcaaact
    10861 ggtctctgag ctctatcgta agcaccgctt gattgaagtt tttctagttc atcatttaga
    10921 ctatacaagt gaccagattc acgaggaagc tgaggtcttg gaacacactg tctctgacct
    10981 gttcgtggaa agactagata aactgctagg tttccctaaa acctgccccc acggaggaac
    11041 tattcctgcc aagggagaac tcctcgttga aatcaataac ctcccactag ctgatatcaa
    11101 ggaagctggc gcctaccgcc tgactcggat gcacgatagt tttgacattc tccattatct
    11161 ggacaagcac tcacttcaca tcggtgacca gctccaagtt aagcagtttg atggcttcag
    11221 caataccttc actatcctca gtaacgacga ggatttacaa gtgaatatgg acattgcaaa
    11281 acaactctat gtcgagaaaa tcaactaatt tctcaagtcc cctaccaacc ctgaaagttt
    11341 tattttcagg gttttatttc tatcatttta ttttactttc atttggtggt agaatatttg
    11401 aaaaaaaaat gggagaatat ttgaaaaaaa gaaaggtagt ttaacctatg aaaaaatcac
    11461 taaaaatttt tgctacatct aaatagtttg acctcttcgg ggttgctttg gtcgttggat
    11521 tgcgattgca tctggttacc tcaactcacg cctcgataaa tttgtagact ggggaccatg
    11581 gacagctctt gtaccctttg gattgatttc cgtaaccaac gttgggattt ccatgttgtc
    11641 cactcgtttc acggggaaat taagcaaatg ggaaaattac tttggtattg ttaataccat
    11701 tttgtccggt gctattgatt atatccttgg aaataaggcg gccatcatta cctatcccgt
    11761 caccttcctc atttatacct ttgctataaa gaaatggaaa gcttcgcaag aaggcagacc
    11821 caaccaaatg agccaaaaac aggtaaaatt ggcggccatc atcatttcca tcatcgcctt
    11881 cctctttgcc tttgtgacca actatatcgg atatgggggc aagatgaatc tccttgccta
    11941 cgtaacaact attgcctttg cactgtccct cattgccaat gctttgaatg cattggcaat
    12001 gagggacagt ggggcttttg gttgatttac aatttcgttc agctgacaaa agctggtatt
    12061 caaggaaact tcgccaatat cggtaaatac atcttttata tcctcaatgc aatcggagct
    12121 ttatttgtct ggaatgatga agaagttaga taatagaagg aaactcaaat aaagaatact
    12181 agccaactta agagaatctc ccctctgcta tttttatcat tttagttgta actcccattc
    12241 ttttagagta gaatgaagtt agataaaaga gggaggtctt gtcatgaaaa aactctatag
    12301 aatccatttt atagcaattg cagttattga tttgctacta tttgcatttt ttatcactag
    12361 actcgagaca tcttttgagt ggctcttgct ctctggttta attttcttcc ttgctcaagg
    12421 actcctgcta tttctcttgg tcgtccgact caaacatcaa ttcgctgaga tttatcctca
    12481 aatcaataaa aagattcgct tctactattt aggggttctc accattgatt ttctattttt
    12541 tgttctctta gccttcatta gttctcagcg tttttcatct cttatgccaa tcatcactgc
    12601 ttgccattct actttttatt atatgacagc tgactaccta agagaaaact atccagactt
    12661 ttacgacaaa cacatctcta acacgtatac tattttatcg atttcttctt ttttagtata
    12721 agacagctcg ttctccagaa atattcatta actttcattt ttatactttt cctttcgatt
    12781 ttcaaaaaca aggggaggga ttaacctccc ttatattaga cataactttt aggatcacaa
    12841 ttgtatgttc ctttcgaaat atccccaaaa atcgtagccc ctgttaaata atgatttctt
    12901 gtagaagcat atgctatttt cttcatcaca tccagtctcg ctacttttag ctcgagatta
    12961 ggattggaaa acatagcatt tttaatatct tccaaatctt gagaactagg tgcgagtaga
    13021 gttacatcgc cctccaatct attagccttg tctatataaa gactaactct ttcagcactt
    13081 tctgttcgca gaaaatttag tacatttcta ttcatttttt tacctcgtat ttttctaatt
    13141 attgattttt tctagaaaca aaatcaattc aacggtgaag ctctagcttt catcttcatt
    13201 cgtacctcct tttgtgtata tctagcttta cataaaccta tgatttagag tatagtttct
    13261 caattattct cagaaactat ggagatgatt taattatcat acaaatcaaa gacctctcct
    13321 tgctcatctt caccgtaact ctcgagttcc tcataaaagt attccaaagc agtttgttct
    13381 tctggtgtca taaagaaact cctttctaaa caatattagt aaaaaatacg ttcttatttc
    13441 catcctaaat aaagtagtgt ttttatcccg taataggttt tatttttgcg gagcattaca
    13501 ctccgcatta atattgatag tattaagaat cattattact taataaaaat tgcgctctca
    13561 aacatagacg tatacaaaat gccctatgta tacgtttcgc ttggaagaca tctaagaaaa
    13621 aattttgtag ttcagacagt aagattaata catctttctc taacataagt gatgtctggt
    13681 actcccaatt attgctctgt tttaacgatg aaaaaggtat agctgtctct tttaatttta
    13741 tccaatctat acgttctttt ttaggaacgt attctctaac agcttgacta acaatactgg
    13801 aatagcttac ttcatagcct aatttattgt aaacatcatc tttaagctta tctaatatct
    13861 gtctggattc agacgctaaa cgcattctaa cattctcttt tcccatatta tcaccccttt
    13921 ctgacgggat tctgttatcc cgtaaattaa gtatataaca atttagaaaa aaaatcaaga
    13981 attaagttga aaaaaattcc acaatctaaa aatttatcat t
//
LOCUS       DAVBHS010000042        14004 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVBHS010000042 DAVBHS010000000
VERSION     DAVBHS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14004
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.042"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(113..1963)
                     /locus_tag="VQ651_000908"
     CDS             complement(113..1963)
                     /locus_tag="VQ651_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET2753926.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMVDFWGLSASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(2031..3170)
                     /locus_tag="VQ651_000909"
     CDS             complement(2031..3170)
                     /locus_tag="VQ651_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2753927.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKGKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDAEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGGLILKIDAHSKVTESFVMNNVAVIQQGEFVCGGPRP
                     TIVKGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLSLLTLLTLLKHK
                     NGFLIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKGTVIYLEK"
     gene            3228..4394
                     /locus_tag="VQ651_000910"
     CDS             3228..4394
                     /locus_tag="VQ651_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HET2753928.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TAMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIDILMTVALFTVDRADKIL"
     gene            4533..4859
                     /locus_tag="VQ651_000911"
     CDS             4533..4859
                     /locus_tag="VQ651_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET2753929.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            4846..5439
                     /locus_tag="VQ651_000912"
     CDS             4846..5439
                     /locus_tag="VQ651_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HET2753930.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEVAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            5432..6343
                     /locus_tag="VQ651_000913"
     CDS             5432..6343
                     /locus_tag="VQ651_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HET2753931.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            6406..7470
                     /locus_tag="VQ651_000914"
     CDS             6406..7470
                     /locus_tag="VQ651_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753932.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(7542..8402)
                     /locus_tag="VQ651_000915"
     CDS             complement(7542..8402)
                     /locus_tag="VQ651_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HET2753933.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHIYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTETDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            complement(8524..9510)
                     /locus_tag="VQ651_000916"
     CDS             complement(8524..9510)
                     /locus_tag="VQ651_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HET2753934.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(9782..11257)
                     /locus_tag="VQ651_000917"
     CDS             complement(9782..11257)
                     /locus_tag="VQ651_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HET2753935.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(11361..12284)
                     /locus_tag="VQ651_000918"
     CDS             complement(11361..12284)
                     /locus_tag="VQ651_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2753936.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(12298..13227)
                     /locus_tag="VQ651_000919"
     CDS             complement(12298..13227)
                     /locus_tag="VQ651_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2753937.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(13546..13896)
                     /locus_tag="VQ651_000920"
     CDS             complement(13546..13896)
                     /locus_tag="VQ651_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753938.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETADKDVLENSQEELQKAVLFLADEKGSEHTEAELI
                     DNLKEVIAKLKANA"
BASE COUNT         4185 a         3025 c         2582 g         4212 t
ORIGIN      
        1 cggcaaggcg acgttgacgt gatttgaaga gattttcgaa gagtataagt ctctacataa
       61 taaaacgcat attaccaagg ttatttaact tgataatatg cgtttttctt ttttattcaa
      121 tgtgggttgt ttgattagca aaggcgataa tagcttgctt caactcatct ccactgagag
      181 tgcggaactc tccaatcttt tgattgatgg attctaaagg catgacatta accttaccaa
      241 cgaaaatctt atccataact tgattatcaa cgagttcggt tgataccaag agttcttcgt
      301 agagtttttc acctgggcgg attccaactt caacgattgg aatttcactt tcagtgtggc
      361 cacttagaag caccatcttc ttggccaagt cataaatctt gactggtttg cccatatcaa
      421 ggataaagac ttccccatct ttggcataag caccagcatg gataaccaga cggctagctt
      481 ctggaatggt cataaagtaa cgggtcatgc ggaagtctgt caccgttaca ggcccacctt
      541 cagcaatctg acgttcaaag actggaatga cactaccacg gctaccaaga acattcccaa
      601 aacgaactgc acagtaggtt gattggctac gttggttaaa gccagtgaca atcaactccg
      661 ccacgcgctt ggttgctccc ataacatttg gtggattgac tgccttatct gtcgaaatca
      721 taaccatctt aggcaccttg gcttcatcaa cggctctagc aacattgtaa gttccacgga
      781 tattgttttt gaaggcttct tttggattgc gctccatcat aggaacatgc ttgtgggctg
      841 ccgcatgata aacaatagca ggtttgtact gctcaaagac ttgcaacaaa cgatcatagt
      901 cttgaatgtc cgcaatcaca ggtacataat caatcccttg gaacttacga atcaattcat
      961 gataaacaag gtagattgag ttttccccat gaccgagcaa gacaatgcgt tcaggattga
     1021 agcgactaac ttgacgacag atttcagaac cgattgaacc tccagctcct gtgactaaga
     1081 tggtcttacc tgtcagttct gcacccagac gcgattcgtc aagacggatt tcctgacgac
     1141 ccaaaaggtc cgtaatatca attttttgga agccagtacc tgcttggtga aggccctgaa
     1201 caacagtttc aaccttaggc atcttgtaac atttgacacc cagcttatta cacatctgca
     1261 agatacgctc atattctgac ggatccagcg acggaatcgc aacgatgaca cgctcgattt
     1321 gatggcgttt ggctaattca ggcagattgt cataagagcc caaaacagga ataccaccaa
     1381 gtttttgacc ctttttctta gaatccttat ccaaaatacc gaccagttct aattcactgg
     1441 ttggatgttg gtaactatcc ataaagagag ccccaccatc accggcacca atcaagaagg
     1501 tccgacggtg ttctccatca ccactacctt ttttgcgtct ggagtagatt aactgccaag
     1561 taatccgtgg caataaaatc aagaaggtac tcaacaagat aaagagaatg ataaaacgga
     1621 tggagaagag tggcaagaag gcataacaga tactatatga caagacactg ctagcagtca
     1681 caccaaaaaa gattttcatg aaatccgtaa tcttgctgta acgactaata ctcgcgctca
     1741 agccccaaaa atcaaccatc aattgataga acaggaaggc caaactcgta tagataatgt
     1801 agtcaacagg tgctggatta atcagcccat aaaataaaat ataagataca atgatggaaa
     1861 ccaccatact gaaaatatca aatattcccc agaaaacctg cttttgttgt ttatttaaaa
     1921 tttccaccag atcaatcaca taatctgtga gttttttatt catagaaatt tactcctcct
     1981 taaaatagtt ctactatatt gttattatag cacattttaa tttatttggc ttatttttcc
     2041 aaataaatta ctgttccctt gtactccttc ttccatttaa atcctctaat taaacctaca
     2101 atcgtcccaa ggccataagc aaagtgaatg gaaaataaaa gaaagggcat cacaattaga
     2161 aatccatttt tatgttttaa taaagtcagc aaagtgagta atgacagtaa tgataaaagt
     2221 agaaaatagg cacctaatag taaagttatg aatacgaatg tgatcggtaa caatgctaga
     2281 ctaaacacaa gactcaaaac aaataaacaa ggaacataat gaaataatga taaacactta
     2341 aactgaacat gacttgtcaa gccaatccac aaaccatttg aatacttttg atgcagcatt
     2401 ttcttgaatg ttggtcgaat atactgataa gatagaatac ttgggctata gcggatttta
     2461 taaccatgtt ctcgaattct ataatgaata tcattatctt cagttcggcc aagttgctca
     2521 tttactaaac caaccttctg gaaaacctct cgtttataca tcccatgaaa aatagaagaa
     2581 acatatctat cctcggaact atttcgataa ttggcaatgc tactgccaaa catattttcc
     2641 tcaacaagat gcaaggtctc tgcccatttt ccttttcctt tgacaatcgt cggtctaggc
     2701 cctccacaga caaattcacc ttgttgaata acagccacat tgttcattac aaaactctca
     2761 gtaacttttg aatgagcatc aatttttaaa ataaggcccc ctacagaatg cttaactccc
     2821 aggttaaaac cactagcttg atttttctta ggattgttat acaatctaat tgagttaaac
     2881 tctgcatctt cttttataaa ttgctgaatg atagctgtgg tcccatctgt ggacatagca
     2941 tttataaata gaatttcaat attctcttta ggataggttt gattttttaa gtcttcaatt
     3001 agaccaggca gatatttttc ttcattgtat gcactgatta caatggatac gagaagactt
     3061 tcaacatttt gagtcactac tactctcctg aattttccct tccaccattc tatcataatt
     3121 ttcaagactt ttccattttt atcttgaaag ccagaaacct tacttgacat tatagtgaaa
     3181 aagccttaaa ataagctgtt attaaaatca gctagaagag atattatatg aaaaagcaat
     3241 cactcttttt tgttccaggt attatcctga ttggtgtttc cttgcgaact ccttttactg
     3301 ttttacccat tattttggga aatatttcgc aaggtctgga ggtagaagtt agttcgcttg
     3361 gtgtcttgac cagcctgcct ctccttatgt ttaccctctt ttcaccattt tctacccgac
     3421 tggctcagaa aatcggcttg gagcatctct tcacctacag cctcttcttc ttgaccatcg
     3481 gctcacttat tcgactaatc aatctgcccc tgctctatct aggaaccttg atggttgggg
     3541 caagtgtcgc agtcatcaat gtcctgcttc ctagtcttat ccaagccaat caaccaaaga
     3601 aaattggttt tctgaccacc ttatatgtaa cgtctatggg gattgcaacg gctctggctt
     3661 cctatctagc tgtgcccatt acacaagcca gttcttggaa aggacttatc ctcctcctca
     3721 cgttactctg tctagcaact tttttggtct ggctcccaaa tcaccgctat aatcatagac
     3781 tagctccaca aaccaaacaa aaaagtcaaa taaaggtcat gcgtaataaa caggtttggg
     3841 caattattat cttttcaggt tttcaatcct tgatctttta caccgccatg acctggttac
     3901 ctaccatgtc tatccatgca ggtctatcca gtcacgaagc tggcttgctg acttctatct
     3961 tatctctgat tagcattcct ttttcaatga ccatcccaag cctgacaacc agtttatcta
     4021 ctcgcaaccg tcagctcatg ctcactctgg tttcactagc tggtgtggtc ggcatttcca
     4081 tgctcttttt cccaatcaat aatttcattt actggcttgc catccatctc ctcatcggaa
     4141 ccgcaaccag cgccctcttc ccttatctca tggtcaactt ttcactcaag accagcgccc
     4201 ctgaaaagac agcccaattg tccggcctat ctcaaacagg aggctatatc ctagcagcct
     4261 ttgggccaac cctctttggt tacagttttg acctattcca ctcttgggta ccagctgtag
     4321 ctgccctctt gctcatcgat atcctgatga ctgtggccct ctttacagtg gacagagcgg
     4381 ataagatcct ttaaacttct agttttagct agaagttttt tatatataaa aattttacac
     4441 atttctcttg acagggcttt cttttagatg tacaatgtat gtgtagaaaa attatatata
     4501 aaaatcttac acattagaaa aggaggtttc ccatgtactt tccaacatcc tctgccttga
     4561 ttgaatttct catcttggct gtactggagc agggtgattc ttatggttat gagattagcc
     4621 aaaccattaa gctgatcgct aatatcaaag aatccacact ctatcccatt ctcaaaaaat
     4681 tggaaggcaa tagctttctg acaacctatt ctagagagtt ccaaggtcgc atgcgcaaat
     4741 actactcctt gacaaacggt ggtatagagc agctcttgac cctaaaagat gaatggacac
     4801 tctatacaga caccatcaat ggcatcatag aagggagtat ccgccatgac aagaactgaa
     4861 tacctgactc agctagaact ctatctcaag aaactacctg aagctgaccg tatcgaagcc
     4921 atggactatt tcagagagct ctttgacgat gctggagtcg aaggagaaga agaactcatc
     4981 gctagtttgg gaactcccaa agaagtggcc cacgaagttc tatccaatct tctcgataaa
     5041 aaaatcaatg aagcacccgc tcaaaaaaat aaccgacaaa ttttacatat cgccttgtta
     5101 gccctccttg cagcacctat cggcattcct ctgggaatcg ccatcctcgt gaccctgttc
     5161 gcaatccttg tagccgcttt gactgtcatt ctggctttct ttgcagtttc catactgggt
     5221 atcatcggcg gattcctatt tttagttgaa agtttcactg tcctcgccca agccaaatca
     5281 gcctttatct tgatttttgg ttctggttta ctggctatcg gtgcttcttc gctagtttta
     5341 cttggcattt cctatgtagc tcgcttcttc ggtctactca ttgttcgtct ggtacaattt
     5401 gttcttaaaa aaggaaagag aggtaatcag catgcgtaaa tggacaaaag gatttctcat
     5461 ctttggtgtg gtgactaccg ttatcggctt tatcctgctt tttgtaggta tccaatctga
     5521 cgggattaag agcctacttt ccatgtccaa agaacctgtc tatgatagcc gtacggaaaa
     5581 gctaaccttt ggcaaggaag tcgaaaacct agaaattact ctccaccaac acacgctcac
     5641 catcacagac tctttcgatg atcaaatcca catttcttac catccatctc tttctgctca
     5701 ccatgatctt atcaccaatc agaacgatag aactctgagt ctcactgata agaaactgtc
     5761 tgaaactccg tttctctctt ctggaattgg tgggattctt catatcgcaa gtagctactc
     5821 tagtcgtttt gaagaagtta ttctccgact accaaaaggg agaactctaa aagggatcaa
     5881 catctcagcc aatcgcggac aaaccaccat cataaatgct agccttgaaa atgcgaccct
     5941 caatacaaac agctatatcc tccgaattga aggaagtcgt atcaaaaaca gtaaactcac
     6001 aacgcccaat atcgttaata tctttgatac agttcttaca gatagtcagc tagagtcaac
     6061 agagaatcac ttccacgctg aaaatatcca agtccatggt aaggttgaac tgactgccaa
     6121 agattatctc agaatcatcc tagaccagaa agaaagccaa cgaattaact gggacatctc
     6181 aagcaactat ggttctatct tccaattcac aagagaaaag cctgaatcaa gaggtacgga
     6241 attaagcaac ccttacaaaa ctgaaaaaac cgatgtcaag gatcaactca ttgcgagatc
     6301 tgatgataat attgatctaa tatccacacc aagcagacgt tgacaaaaac gcttacatct
     6361 gctactatgg aagcatattt atctaactaa aaaggaggtt ctaccatgaa acaagaatgg
     6421 tttgaaagta atgattttgt aaaaacaaca agcaagaaca agcctgaaga gcaagctcaa
     6481 gaggttgcag acaaggctga agaaacgata gccgatctcg atacaccaat tggaaaaaat
     6541 actcaagtag aggaggaagt ctctcaagct gaagtcgaac tggaaagcca gcaagaagag
     6601 aaaattgaaa ctcctgaagg cggtgaagcg agaacagaaa tagaagaaaa gaaggcatct
     6661 aattctactg aagaagagcc agacctttct aaagaaacag aaaaagtcac tatagctgaa
     6721 gagagccaag aagctcttcc tcagcaaaaa gcaaccacga aagagccact tcttatcagt
     6781 aaatctttag aaagtcctta tatccccgac caagctccaa aatctaggga taaatggaaa
     6841 gagcaagtgc ttgatttttg gtcttggcta gtggaagcga tcaaatctcc tacaagtaag
     6901 ttggaaacaa gtatcacaca cagttacaca gcctttctct tgctcattct gttttctgca
     6961 tcttcctttt tctttagtat ctatcacatc aaacatgctt actatggaca tatagcaagc
     7021 attaacagtc gcttccctga gcagctagct cctttaactc ttttttctat catctctatc
     7081 ctagtagcga caacactctt cttcttttca ttcctcttgg gtagtttcgt tgtgagacga
     7141 tttatccacc aggaaaagga ctggacgcta gacaaggttc tccaacaata tagtcaactc
     7201 ttggcaattc caatcttcct cactgctatt gctagtttct ttgctttctt tgatagccta
     7261 cgatttacgg ccctcttgtg tgtgattagc attggaatca ttctgcttgc cagtctccat
     7321 atcattacaa gaccaagtca agcaagtgaa accgactcct tctatcaatt attcttgtct
     7381 gtccttgtga acggagttat tatcctcctc ttctttgtag ctgaagtggc actgattgga
     7441 gattatcttc gtatcttggc ctttctttaa aattcaatcc tgtcatcctt ggcaggattt
     7501 tttctatgtc aaaaaagcaa gtcgattgac ctgcttctgc tttacttttc ttgtgctaat
     7561 gttttaaaat ctttgtctaa tatgggttgc acttctattt gattggcgtc tagttggtgg
     7621 taaaaagatg ggggtaatac ctctaagaat tttgcatagt ccaagcgagc aatggcttca
     7681 tagtcttctg gtttagagcc atctcctgtg atttgaacca agtcaatctc ccactgcact
     7741 tccttatcca ccaattgctc aatataggct gcaggaacaa acggttctct cggtaaaact
     7801 gtcttgactc cttgagctag atggtaaacg ccatgcactt ttccttctcc atcctgatag
     7861 aaggaaactc tcgcaaagta ggcatctgtc tcttgaaccg cacgcacacg cgcttcaaac
     7921 tgagccaaac catctgcctc taaaaagctt ttcaagttct cacgactaaa gaaaatagga
     7981 ttaaaaattc cttggcaaat cgtaaaacga gctacagggt ctgctagata ggcttgaata
     8041 cttgcttgga aataagcttc aaaatcaaaa ccatctagcc cttcaatatc agtccctgta
     8101 taagcaagca aagcatctaa aaatgacttg ataatctgcc agtctgtctc tgttagtagg
     8161 tcaggaagat cgacacggta agccttttga ccatcagcat aactgacctt aaaaagaaat
     8221 tgtgattgcc ccactatcgc acactcaata tactcgagac gattaagagg ctgacggaga
     8281 tagactacat catagatatg tgactccaaa ccgtctagca atgccaagat cgacttggca
     8341 ttcaaaatct cttgttctcc taaaatgctc tgtttatttg gaataaaaaa tgttttcgcc
     8401 ataactggac tcctttgaaa tcgcttacat acagtatacc ttatttttct gaaagataca
     8461 gaaacaaact ttagattttt gagtacaaag catagaaaaa cagcccctga ggactgtttg
     8521 atcttataca gtagctgctt gatccaagct ttcaccgata gcggctaggc gctcgatcac
     8581 ttcagcttgt gtcaattcat tttttgaaac atagcggtta cgtgggtgaa cacggcactc
     8641 gtgtgagcat ccacgaaggt acttgtcttc attttcttct gatgtcaaga tacgacggtt
     8701 acagaaggga tttccacagt tgacataacg ttcacatggt gttccatcaa accagtcttt
     8761 ccctacgatg gttgggttga catggttgac atcaacggcg atacgctcgt caaatacgta
     8821 catttttcca tcccaaagct caccttgaac ttctgggtct ttaccgtaag ttgcgattcc
     8881 tccgtgcaat tggccgacat ctttgtagcc ttcacggacc atccagcctg agaatttctc
     8941 acagcgaacg ccacctgtac agtaaaccac gacacgcttg tccatgaatt tttccttgtt
     9001 atcacggacc cattgtggta actcacggaa gttgcgaata tctgggcgaa tagctccacg
     9061 gaaatgtcct aggtcgtact cataatcgtt acgtgtgtca aggacaacgg tatctttatc
     9121 aagaagcgct tctttgaact cttttggaga caagtaagca cctgttgttt caagtgggtt
     9181 gatgtcattg tcaaagtcgt tgtcttccaa accaaggtgg acaatttctt tcttgtagcg
     9241 aacaaacatc ttcttgaagg cttgttcatt ttcttcgtca atcttgaacc agagttcttc
     9301 cattcctgga aggctgtgaa cgtagtccat gtatttttga gttgtttcat agtcacctga
     9361 aactgttccg ttaattccct cgtcagcgac taggatacgg cctttaagac cgattgattt
     9421 acagaaagcc aagtggtctg cagcaaattg ctctgcattt tcaattggag tataaaggta
     9481 gtaaagtaag acacgaatat cttttgccat aagatttgtt ctcttttcta ttcttaaatt
     9541 ttctaaattt tttatttatc catactagta tacctgcttg agaaaacgaa tgcaatttat
     9601 ttgactggaa attgtagagg attctcgaaa tttcctgaat ggttaaaata aggacaagag
     9661 aaaacatgga tatctatatc cttgtgccaa aaaaaccact gccctcccca gaccaacctg
     9721 aggaaagcag tgattcttat tttaggagtt aggaatgaat acacgaaatc aatttagctg
     9781 attatttttt gtttttcaag aattcatcgt attgtttttg catttcgttc aatacttttt
     9841 cgtaggcacc ttcagatttc aatttttcca tcaattctgg aatcgcttta tctgggtcta
     9901 cagtaccagt gttgatagct gtatcaaatt gttgcattgt gttagcaata gctgagattt
     9961 cagatttcac attgtcagta ttgaagataa atccaagcgc tggagattct ttagcttctg
    10021 ccaattcttt cttagaattt tcgatttgtt ggtctgtaac gttttcgttg atgtaaagga
    10081 tccagttgtt accagtgttc catccaccca tgtgagtgtt tcctttgtag ccatcaagaa
    10141 cgcgaacacg gttttcttta ccttcaattt tttcccagtt cttgccttct ggaccgtaaa
    10201 caagaccgtt caaaagttct gggttcgtat tcaagaggtt caagatttcc attgattttt
    10261 ctttgttctt agagttgttt gagatgacaa agttagcaac ttgtgttgtt tggtttttct
    10321 tgatgaagtt agtgattggt ttgatttgga tatctttgtt ggcaacacgt gaaagcaagc
    10381 tgttaccgta gtcagctggt cctactgttt cttcacgaac gaaccaagta tcttgttgaa
    10441 ggtcaaatga agtatcgctt gttgcgacgt cttttggaat gtagccagct tcatagaatt
    10501 tgtgaagagt cttcaagtgt tctttgaaac gaggcacttc gtaacggttt acaactttag
    10561 tagtatcgcc ttcaaggtca ataacgaatg gaagcccgtt tgctactggg tagtcaaaat
    10621 tatcagatgg gatgaaaact ttaccaatag caaatggtac tacgtctgga gctttttctt
    10681 tgatttgttt caagactggc tcaagagttt cgtaagaagt aacacctgaa atatcgatac
    10741 catatttggc aaggagagtt ccgttgaagg caaagttttg agatgatgct acgttggctg
    10801 caactggtac agcataaatc ttaccattta cagtattacc cttgatataa gctggatcaa
    10861 gtgccttgta aaggtcttta ccttcttttt tgtacaattc tgtcaagtca gcataagcac
    10921 ctttttgagc atttacaata tagttatctg caaaggcgat atcatagttt tcaccagatg
    10981 atgtgataac tgacattttc ttaccatagt caccccagcc aaggtattgg atatccaatt
    11041 tggcaccaac tttttcttcg atgattttgt tggcatttgc taacaattca tccaagttat
    11101 ctggtttgtc accgatttgg tacattttga taacaggttt gtcacctgaa tcagcagctt
    11161 ttttgctgtt acctgtcaaa tttccacaag cagcaagacc tgcagccaga gcgactacac
    11221 tagcagatgc aaaagcatat tttttccagt ttttcatgat aaaaactcct ttttttattt
    11281 ttaaacttat aaacaatgta atgatcttaa cttcacacaa gggaagttgg gaactgagaa
    11341 atgttctttt tcaataagca ctattctttc acaccaccga tagtcaaacc ttttacaaag
    11401 tagcgttgga agaatggata caaaatcgcg attggaaggg ttgctaccac aaccatggcc
    11461 atacgacctg tttctttcgg tagagcaact cccagttgac cagtcaagcc gaccgctttg
    11521 gcaatgtagt ccatattttg ttggatttgc atgagcaaat attgcaatgg atacaagtta
    11581 tcactcttga tgtaaagaag ggcgttgaac cagtcattcc agaaaccaag agctgtcaaa
    11641 agcgtgatgg ttgcgatacc tggtagtgac aatggcaaac agatttggaa gaaaatccga
    11701 gcctcactgg caccatcgat acgagccgat tcgagaatgg cttctggaat ggtcttcttg
    11761 aaaaaagaac gcatcaagat gatattgaat ggtgagagaa gcattggaac aatcaaggcc
    11821 caaacagtgt caccaagctg aagtacacgg gtcaccatga tataacctgg taccaaacca
    11881 gcgttgaaca acatactgag aaggacgaag atggtaaaaa atctgcgata cttaaaggtt
    11941 gtccgtgaaa tagcgtaggc ataggttgtt gtgataaaga catttgtcaa tgtcccaact
    12001 acggttacaa agacagagat gaagagggct tgtaggattt tatccttaaa ctgtgccaaa
    12061 aactcaaaac cgtctaagcc aaattgggat gggaagaagc tatagccgta ttggaggagg
    12121 cttttctcgt ctgtcactga aataatgata acgaatacaa aaggtaggat acaagagagg
    12181 gcaatcaaac ccgaaatgat actgaagaag atatctgctt tcttactgaa ggagtgaatg
    12241 ccgacattat caattttttc ttttttaatt ttcttttttg ccatattctc ctcctttcta
    12301 gaacaaagct gagtttggat cgactcgtct tgcaagcaag tttgatagga taaccagaat
    12361 caaaccaaca acggattggt aaagaccggc tgctgcagcc ataccgatat ctgctgtctg
    12421 agtcaaacca ttaaagacat atacgtccaa aacgttggtt acattgtaaa gctgaccagc
    12481 attgtgtggg atttgataga agagaccgaa gtctgcgcgg aagatatttc cgactgcaag
    12541 gatggtcaat acagttacaa gcggagtcaa ctgaggaatg gttacgttgc gaatacgttg
    12601 ccacttgcta gctccgtcca ctgtcgctgc ttcgtagtag gttggatcaa ttcccatgat
    12661 cgtcgcatag tacatgacac tgctatatcc aaagcctttc caaataccta ggaaaagtag
    12721 gagatagggc cagatgccca ggtcagcgta gaaattgact tctttgagac caagactttc
    12781 caatagatga ttgaacaccc ctttatcaat atttaggaag gcatctgtaa agaaactgat
    12841 gataacccaa gacaagaagt aagggaacaa catagaagtt tgaaaaatct tcaccattct
    12901 cttagaacgg agctcgctga ggataatggc aatccctaca gatacaacta aacctagaaa
    12961 gataaagcca agattgtaga ggacagtatt tcgtgtgata ataaaggcgt ctcttgaact
    13021 aaataagaat ctgaaattat cgagtccgac ccatttacta tttatgatac tatctatgaa
    13081 accattactg gtcatgtggt agtctttgaa ggcaaccacg ttcccaaata ctggaatgta
    13141 aaagaataga atcaaccaga gtgcccctgg caaaaccatc aagagaaaga tccagttgtc
    13201 tctcaatgtt tttgaaaact ttttcataat ttcctccctt tttattttga tatccatcta
    13261 aaaattcttt tttagacttt tgataacgat tacattatta gtatactcct atttgcaggt
    13321 taggttaaac tcctaattat agaaaaaact ccacaaatta tgtagcagat ttaaaacttt
    13381 atcaccacta tcaaacaaat gtcctaaatc aattgtttat tttatctcta ttagcccagt
    13441 gatggcgtca ctctgttata agcatccaac aacggggtat actgaaaaat ctccagacta
    13501 gggaactcag cgatagttcc taatctggag atttttaata tgttattagg cgtttgcttt
    13561 caacttagca ataacctctt taagattatc aatcaactct gcttcagtat gctcagagcc
    13621 tttttcatct gccaagaaca aaactgcttt ttgaagttct tcttgagagt tttcaaggac
    13681 atccttatct gctgtttctt tgcccgctgt ttgtcctcca tccttagttg ctgactggct
    13741 ttccttaatg gactctaggg aagcaatggc atctttgact gtttgcaaga tatcacgtaa
    13801 accttgctct gtcaaactat catctgcaaa agctttctta gcctctgcca aaaccagacg
    13861 tgctgaatct gtggtaggat tcgatacacc tgtcaatgat ctcaaaagat tttctaaagt
    13921 ttgagtctgc ttactaatac tagactaaaa tcaaaaagca ttatataaca gtgatatgaa
    13981 atcaactaaa gaagaaatcc aaac
//
LOCUS       DAVBHS010000043        13998 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVBHS010000043 DAVBHS010000000
VERSION     DAVBHS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13998
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.043"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(317..2008)
                     /locus_tag="VQ651_000921"
     CDS             complement(317..2008)
                     /locus_tag="VQ651_000921"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET2753939.1"
                     /translation="MNTKELIASELASIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            2144..2590
                     /gene="argR"
                     /locus_tag="VQ651_000922"
     CDS             2144..2590
                     /gene="argR"
                     /locus_tag="VQ651_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2753940.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            2641..5175
                     /gene="mutS"
                     /locus_tag="VQ651_000923"
     CDS             2641..5175
                     /gene="mutS"
                     /locus_tag="VQ651_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET2753941.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKLDSQNNFLVAIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSCQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPALAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLITGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTENPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            5322..7016
                     /gene="patA"
                     /locus_tag="VQ651_000924"
     CDS             5322..7016
                     /gene="patA"
                     /locus_tag="VQ651_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HET2753942.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDVPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(7346..7615)
                     /locus_tag="VQ651_000925"
                     /pseudo
     CDS             complement(7346..7615)
                     /locus_tag="VQ651_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            7807..9573
                     /gene="patB"
                     /locus_tag="VQ651_000926"
     CDS             7807..9573
                     /gene="patB"
                     /locus_tag="VQ651_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HET2753943.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTHVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            9712..10401
                     /locus_tag="VQ651_000927"
     CDS             9712..10401
                     /locus_tag="VQ651_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET2753944.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLDEL"
     gene            10405..10584
                     /locus_tag="VQ651_000928"
     CDS             10405..10584
                     /locus_tag="VQ651_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753945.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            10571..11920
                     /locus_tag="VQ651_000929"
     CDS             10571..11920
                     /locus_tag="VQ651_000929"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET2753946.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            12044..13504
                     /locus_tag="VQ651_000930"
     CDS             12044..13504
                     /locus_tag="VQ651_000930"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET2753947.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            13518..13952
                     /locus_tag="VQ651_000931"
     CDS             13518..13952
                     /locus_tag="VQ651_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2753948.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNTYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT         4089 a         2703 c         3164 g         4042 t
ORIGIN      
        1 tttggttagg tgaattagtg aagcgtttag gcaagtgtct ctggttacga cgtcatggac
       61 tctaaatcga ttatatttag gggtcatgac tagtgaagca gttagctagt tcgcatataa
      121 gcggctagcg tctaacaatt aggaacttta gttccaataa ctttaagatt acgacgtttt
      181 aggacataaa tcgatcatat ttatgtccta aaactagtga agtatgtcct aaaactagtg
      241 aagcgcctag ccaaagtccg aataggattt ggcgttagtt acttagattg ctttgcaatc
      301 aagtaacttt ggcgatttac atcttctctg gcgcttctac tccaagcaag cgaagggctt
      361 ctttgagaac gactgcggtt gcgtagctga gggctagacg gctgtcgcgt tctgggcttt
      421 catccaagat acgtgtatgt gcatagtatt tgttaaagga ttgagccagg ctaattgcaa
      481 atttagcaat gatagaaggt tcaaagttat ctgccgcacg gttgataata cgtgggaagt
      541 cttgaatgag tttaatgatt tcccagcttt cagtatcatt caagctatag ttgccagctg
      601 tttctggttt gaaatcggct ttgcgtaaga tagattggat acgagcgtag gcatattgaa
      661 cgtaaggtcc agtttcaccc tcgaaggata ccatagcctc taggtcgaag tcgtatccat
      721 ttgtacggtc ggttttgagg tcatagaatt taatggctcc aaccccaaca gcatgtgcta
      781 cttggtcttt gttttctagt tcaggatttt tagcctcgat ttggaccttg gcacggctaa
      841 cagcctctgc aacagtaggc tctagcaaga tgacattccc tttacgagta gagagtttct
      901 tcccttcttt tgtaaccaaa ccaaaaggaa cgtgagtaat gtcgtcactc cagtcgtagc
      961 ccatctcttg caagacagct ttgagctgtt taaagtgggc agattgttct tgaccaacga
     1021 catagataga tttagcaaat tggtattcgt ttttacggta gagggctgca gccaagtcac
     1081 gtgtgatata gagagttgca ccatcagact tcttgatgag ggctggatgt tcaattccgt
     1141 atttctcaag attcacaact tgggcacctt ctgattcaag aagtagtcct ttttcagaaa
     1201 gaatgtctac aactgcatcc atcttatcat tgtagaaggc ttctccgtta tagctgtcaa
     1261 attcaacctt caattcattg taaaggcggt taaattccac taaactttca tcgcggaacc
     1321 attgccaaag agcgagagct tcctcatctc cattttcaag tttacggaac cattcgcgcg
     1381 cttcttcatc caagctaggg tcattttcag cttcagcgtt gatgcggaca tagagtttaa
     1441 ggagttcatc gattggatga gcttttacag cttcttcgtc gccccatttt ttgtaggcaa
     1501 caatcaacat cccaaattgt ttaccccagt ctcccaaatg gttgaccttg accgtttgat
     1561 aaccgatttt ttggaaaata tgtgacaagc tatctccgat aacagttgaa cgcaggtggc
     1621 caatagaaaa tggtttagcg atattcggac tagacatgtc gataacaaca ttttcttgtt
     1681 taccaatatt ttggtcagca tagtgttctt tttcagtggt aacagcttgc aatacttgag
     1741 cagaaatggc agatttatca aggaaaaagt taacgtaagg tcctgttgcg acaacttttt
     1801 caaaggcttg gctgttcatt ttttcagcca gttcagccgc aatcatttgt ggtgctttac
     1861 gttcgacttt ggcaagagaa aaagcaggga aagcgatgtc acccatttct gagtttttag
     1921 gggtttccag taactttaaa atagcctctt ggtccaggct atcaatgatg ctagccaatt
     1981 cgctagcaat caattctttt gtattcatta agagctcctt tttggacttt tctactattt
     2041 tatcacaatt ttaaagaaag aagaaaaaat ttttgaaatc tcctgttttt ttggtataat
     2101 atggttataa atatagttat aaatatgcac gcaagaggat tttatgagaa aaagagatcg
     2161 tcatcagtta ataaaaaaaa tgattactga ggagaaatta agtacacaaa aagaaattca
     2221 agatcggttg gaggcgcaca atgtttgtgt gacgcagaca accttgtctc gtgatttgcg
     2281 cgaaatcggc ttgaccaagg tcaagaaaaa tgatatggtg tattatgtac tagtaaatga
     2341 gacagaaaag attgatttgg tggaattttt gtctcatcat ttagaaggtg ttgcaagagc
     2401 agagtttacc ttggtgcttc ataccaaatt gggagaagcc tctgttttgg caaatattgt
     2461 agatgtaaat aaggatgaat ggattttagg aacagttgct ggtgccaata ccttattggt
     2521 tatttgtcga gatcagcacg ttgccaaact catggaagat cgtttgctag atttgatgaa
     2581 agataagtaa ggtcttggga gttgctctca agacttattt ttgaaaagga gagacagaaa
     2641 atggcgatag aaaagctatc acccggcatg caacagtatg tggatattaa aaagcaatat
     2701 ccagatgctt ttttgctctt tcggatgggt gatttttatg aattatttta tgaggatgcg
     2761 gtcaatgctg cgcagattct ggaaatttcc ttaacgagtc gcaacaagaa tgccgacaat
     2821 ccgatcccta tggcgggtgt tccctatcat tctgcccaac agtatatcga tgtcttgatt
     2881 gagcagggtt ataaggtggc tatcgcagag cagatggaag atcctaaaca agcagttggg
     2941 gttgttaaac gagaggttgt tcaggtcatt acgccaggga cagtggtcga tagcagtaag
     3001 ctggacagtc agaataattt tttggttgcc atagaccgcg aaggcaatca atttggccta
     3061 gcttatatgg atttggtgac gggtgacttt tatgtgacag gtcttttgga tttcacgctg
     3121 gtttgtgggg aaatccgtaa cctcaaggct cgagaagtgg tgttgggtta tgacttgtct
     3181 gaggaagaag aacaaatcct cagctgccag atgaatctgg tactctctta tgaaaaagaa
     3241 agctttgaag accttcattt attggatttg cgattggcaa cggtggagca aacggcatct
     3301 agtaagctgc tccagtatgt tcatcggact cagatgaggg aattgaacca cctcaaacct
     3361 gttatccgct acgaaattaa ggatttcttg cagatggatt atgcgaccaa ggctagtctg
     3421 gatttggttg agaatgctcg ctcaggtaag aaacaaggca gtcttttctg gcttttggat
     3481 gaaaccaaaa cggctatggg gatgcgtctc ttgcgttctt ggattcatcg ccccttgatt
     3541 gataaggaac gaatcgtcca acgtcaagaa gtagtgcagg tctttctcga ccatttcttt
     3601 gagcgtagtg acttgacaga cagtctcaag ggtgtttatg acattgagcg cttggctagt
     3661 cgtgtttctt ttggcaaaac caatccaaag gatctcttgc agttggcgac taccttgtct
     3721 agtgtgccac ggattcgtgc gattttagaa gggatggagc aacctgctct agcctatctc
     3781 atcgcacaac tggatgcaat ccctgagttg gagagtttga ttagcgcagc gattgctcct
     3841 gaagctcctc atgtgattac agatggggga attatccgga ctggatttga tgagacttta
     3901 gacaagtatc gttgcgttct cagagaaggg actagctgga ttgctgagat tgaggctaag
     3961 gagcgagaaa actctggtat cagcacgctc aagattgact acaataaaaa ggatggctac
     4021 tattttcatg tgaccaattc gcaactggga aatgtgccag cccacttttt ccgcaaggcg
     4081 acgctgaaaa actcagaacg ctttggaacc gaagaattag cccgtatcga gggagatatg
     4141 cttgaggcgc gtgagaagtc agccaacctc gaatacgaaa tatttatgcg cattcgtgaa
     4201 gaggtcggca agtacatcca gcgtttacaa gctttagccc aaggaattgc gacggttgat
     4261 gtcttacaga gtctggcggt tgtggctgaa acccagcatt tgattcgacc tgagtttggt
     4321 gacgattcac aaattgatat ccggaaaggg cgccatgctg tcgttgaaaa ggttatgggg
     4381 gctcagacct atattccaaa tacgattcag atggcagaag ataccagtat tcaactgatt
     4441 acagggccaa acatgagtgg gaagtctacc tatatgcgtc agttagccat gacggcggtt
     4501 atggcccagc tgggttccta tgttcctgct gaaagcgccc atttaccgat ttttgatgcg
     4561 atttttaccc gtatcggagc agcagatgac ttggtttcgg gtcagtcaac ctttatggtg
     4621 gagatgatgg aggccaataa tgccatttcg catgcgacca agaactctct cattctcttt
     4681 gatgaattgg gacgtggaac tgcaacttat gacgggatgg ctcttgctca gtccatcatc
     4741 gaatatatcc atgagcacat cggagctaag accctctttg cgacccacta ccatgagttg
     4801 actagtctgg agtctagttt acaacacttg gtcaatgtcc acgtggcaac tttggagcag
     4861 gatgggcagg tcaccttcct tcacaagatt gaaccgggac cagctgataa atcctacggt
     4921 atccatgttg ccaagattgc tggcttgcca gcagaccttt tagcaagggc ggataagatt
     4981 ttgactcagc tagagaatca aggaacagag aatcctcctc ccatgagaca aactagtgct
     5041 gtcactgaac agatttcact ctttgatagg gcagaagagc atcctatcct agcagaatta
     5101 gctaaactgg atgtgtataa tatgacacct atgcaggtta tgaatgtctt agtagagtta
     5161 aaacagaaac tataaaacca agactcacta gttaatctag ctgtagcaag gagacttctt
     5221 tgacaattct ccactttttt gctagaatga catcacacaa acagaatgaa aaggagctga
     5281 cgcattgtcg ctcccttttg tctatttttt aaggagaaag tatgctgatt cagaaaataa
     5341 aaacctacaa gtggcaggcc ctggcttcgc tcctgatgac aggcttgatg gttgctagtt
     5401 cacttctgca accgcgttat ctgcaggaag tcttaggcgc cctccttact gggaaatatg
     5461 aagctattta tagtatcggg gcttggttga ttggtgtggc cgtagtcggt ctagttgctg
     5521 gtggactcaa tgttgtcctc gcagcctata ttgcccaagg agtttcatcc gaccttcggg
     5581 aggatgcctt ccgtaaaatt caaacctttt cttatgctga tattgaacaa tttaatgcgg
     5641 gaaatctagt cgttcgaatg acaaatgata tcaaccagat tcagaacgtt gtcatgatga
     5701 ccttccagat tcttttcaga cttcccctct tgttcatcgg ttcgtttatc ctagcggttc
     5761 aaaccttacc ttctctgtgg tgggtgattg ttctcatggt agtcttgatt tttggtttga
     5821 ctgctgtcat gatgggaatg atggggcctc gttttgccaa gtttcaaacc cttcttgagc
     5881 gcatcaatgc cattgccaag gaaaatttac gtggcgttcg tgtggtcaag tcctttgtcc
     5941 aagaaaaaga gcaatttgct aagtttacag aggtctcaga cgagcttctt ggtcaaaacc
     6001 tttacattgg ttatgccttt tcagtagtgg aaccctttat gatgttggtt ggttacgggg
     6061 cggtcttcct ctctatttgg ctggtcgcgg gaatggttca gtcggatccg tctgttgttg
     6121 gttccatcgc ttcttttgtt aattacctaa gccagattat ctttaccatt gttatggttg
     6181 gatttttggg aaattctgtc agccgtgcca tgatttccat gcgtcgtatt cgagaaattc
     6241 ttgacgcaga gccagctatg accttcaagg atgtcccaga tgaagagttg gttggaagcc
     6301 ttagctttga aaatgtgacc tttacctatc caatggacaa ggaaccgatg ctgaaagatg
     6361 tgagctttac tattgaacct ggtcaaatgg ttggtgtagt tggagcgact ggtgcaggaa
     6421 agtcaacctt ggctcaattg attccacgtc tctttgatcc acaggaaggg gctatcaaaa
     6481 tcggaggtaa ggatattcga gaagtgagtg aaggaaccct gcgtaaaaca gtttccatcg
     6541 ttctccaacg tgccattctt tttagtggaa cgattgcaga taacttgaga caggggaagg
     6601 gaaatgctac tctatttgaa atggagcgcg cagccaatat tgcccaggct agtgaattca
     6661 ttcatcgtat ggagaaaacc tttgaaagtc cagttgaaga acggggaacc aatttctctg
     6721 gtggacaaaa acaaaggatg tcgattgcgc gtgggattgt cagcaatcca cgtattctga
     6781 tttttgatga ttcgacctca gccttggatg ccaaatcaga gcgcttggtg caagaagctt
     6841 tgaataagga cttgaagggg acgacaacca ttattattgc tcaaaaaatt agctcggttg
     6901 tccatgcaga caagatcttg gttctaaatc aaggacgatt gattggtcaa ggcacgcatg
     6961 cagacttggt tgccaacaat gccgtttacc gtgaaatcta tgaaacacag aaatgaaaga
     7021 caaactataa gaaaagtcaa tagttttatc taaactattt cttatttcaa tttgatgatt
     7081 tggcgatgat tttagagcac ggcaaaaagc ccttgaaaaa gtccattttt tcaaaggtaa
     7141 tcctgtgttg atttcagaaa ttacatcact ttttgttcgt caaatggcag ctctttttta
     7201 ggatataaaa cagggttcgg ataagttttt ttgcaaggtg gatgatggct acattgtaat
     7261 gttttccttg ttataactta gtcttaagat aggccttaaa agcaggtgaa aagtgagggc
     7321 atgctttggc agcttgtatg agtacctacc gcagatgagg ggaaccccgt ttgaccattc
     7381 ttccagctaa atcaatctga cctgactgat aaatagaaga atccagtcca gcgaaagctt
     7441 gtaattgagc aggattatca aaggcatgaa tatttcgaat ctcggctaaa atgaccgccc
     7501 ctaaacgatt cccaatccca gtaaccgtcg tgatgaccga gttgaactca gccatcaagt
     7561 cattgacaca tttttccgcc ttgtcaatga gcctcttgta atgtttgatg atttcattac
     7621 acgagataaa acgtctatgc gttatcaaac tcattaccaa ttaaaacaaa aagctgtggt
     7681 tagatccttt cggaaatcgt caagcgattg gaggaaatga actaatccac agcggcttat
     7741 tccaagtata ccacttgggc tttggcagta gctaactgcg ctaaatataa tataaggagg
     7801 agtaaaatga agacagttca atttttttgg cattatttta aggtctacaa gttctcattt
     7861 gtagttgtca tcctgatgat tgttctggcg acttttgccc aagccctctt tccagtcttt
     7921 tctggacaag cggtgacgca gctagccaat ttagttcaag cttatcaaaa tggcaatcca
     7981 gaacttgtat ggcaaagcct atcaggaatc atggtcaatc ttggcctgct ggttttggtt
     8041 ctatttatct ctagtgtaat atacatgtgt ctcatgacgc acgtgattgc agaatcgacc
     8101 aacgagatgc gcaaaggcct ctttggtaag cttgctcagt tgacggtttc tttctttgac
     8161 cgtcgacaag atggcgatat cttgtctcat tttaccagtg atttggataa tatcctccaa
     8221 gcctttaacg aaagcttgat tcaggtcatg agcaatattg ttttatacat tggtctgatt
     8281 cttgtcatgt tttcgagaaa tgtgacgctg gctctcatca ccattgccag caccccattg
     8341 gctttcctta tgctgatttt catcgtgaaa atggcacgca aatacaccaa cctccagcag
     8401 aaagaggtag ggaagctcaa cgcctatatg gatgagagca tctcaggcca aaaagccgtg
     8461 attgtgcaag gaattcaaga ggatatgatg gcaggatttc ttgaacaaaa tgagcgcgtg
     8521 cgcaaggcaa cctttaaagg aagaatgttc tcaggaattc ttttccctgt catgaatggg
     8581 atgagcctga ttaatacagc catcgtcatc tttgctggtt cggctgtact tttgaatgat
     8641 aagtctattg aaacaagtac agccctaggt ttgattgtta tgtttgcaca attttcacag
     8701 cagtactacc agcctattat ccaagttgca gcgagttggg gaagccttca gttggccttt
     8761 actggagctg aacgaattca ggaaatgttt gatgcagagg aggaaatccg acctgaaaag
     8821 gctccaacct tcactaagtt gcaagaaagt gttgaaatca gtcatatcga tttttcatac
     8881 ttgcctgata aacctatttt gaaagatgtc agcatttctg cccctaaagg ccagatgaca
     8941 gcagttgttg ggccgacagg ttcaggaaaa acgactatta tgaacctcat caatcgcttt
     9001 tatgatgttg atgctggtgg tatttatttt gatggtaaag acattcgtgg ctatgactta
     9061 gatagtctta gaagcaaggt gggaattgta ttgcaagatt cggtcttgtt tagcggaacg
     9121 attcgagaca atatccgatt tggtgtgcca gatgctagtc aggaaatggt tgaggtagca
     9181 gcaaaagcaa cccacattca cgactatatc gaaagtttgc ctgataagta cgatactctt
     9241 attgatgatg accagagcat cttttcaaca gggcagaagc aattgatttc aatcgctcga
     9301 accctgatga cagatccaga agttctcatt ctcgatgaag caacttcaaa cgtagatacg
     9361 gtgacagaaa gcaagattca gcatgccatg gaggtggttg tagcaggtag aactagtttc
     9421 gtcattgccc accgcttgaa aaccattctc aatgcagatc agattattgt ccttaaagat
     9481 ggagaagtca ttgaacgtgg taaccaccat gaacttttga agctaggtgg cttttattca
     9541 gaactctatc acaatcaatt tgttttcgaa taagaaagaa gttgtcctat gtgggcagct
     9601 ttttcttgtc cataaaaaat gtttatcaca gccttaaaaa aaacatatta gacgaaagtc
     9661 attttgagtg atatgatagg actatcgtta gcattcgaaa ggagaggcat catggctaga
     9721 acggttgtag gagttgctgc aaatctatgt cccgtagacg cagaaggcaa aatcattcat
     9781 tcatctgtat cttgtagatt cgcagagatc attcgtcaag tcggtggtct ccctttagtc
     9841 attcctgttg gtgatgagtc agttgtacgt gattatgtgg aaatgattga caaactcatt
     9901 ttgacaggag gccaaaatgt tcatcctcag ttttatggag agaaaaagac cgtcgagagc
     9961 gatgattaca atctggtccg tgacgaattt gaattggcac tcttgaagga agcgcttcgt
    10021 cagaataaac caattatggc aatctgtcgc ggtgtccaac ttgtcaatgt tgcctttggt
    10081 ggaaccctca atcaagaaat cgaaggtcac tggcaaggac tacctttcgg gacatctcac
    10141 tctattgaga cagtagaagg aagcgtggtg gctaagctat ttgggaaaga aagtcaggtc
    10201 aactcagtcc atcgtcaaag cattaaagat ttggcaccta atttccgtgt aactgctatt
    10261 gattcaagag accaaactat tgaagcgatt gagtctatcg acgagcaccg cattatcggt
    10321 ttacagtggc atccagagtt tctggttaat gaagaagatg gcaatttaga attatttgag
    10381 tatttattgg atgaactgta acaattggaa catctggtcg ttctttcttt tattttttca
    10441 aaatttttag aatgtggtat tttacgcaaa cgtttgaatt ctgataaaaa ttggagaaat
    10501 tcgacaaaaa aacttgaaaa aaacgaaggt aagcgttatg atagaaaaga agaaatattg
    10561 gaggaagaac atgtcacata ttaaatttga ttattcaaaa gttttagaca aatttgttgc
    10621 accacatgaa gtggaataca tgcaatcaca agtaacagca gcagatgaat tgatccgtaa
    10681 aggaactggt gctggtagcg acttcttggg atggttggac cttcctgaaa aatacgaccg
    10741 cgaagaattt gaccgcatct tgaaagctgc tgagcaaatc aagtcagaca gcgatgtttt
    10801 ggttgtgatc ggtattggtg gatcttacct tggtgcaaaa gcagcaatcg acttcttgaa
    10861 tcaccatttt gctaacttgc aaacaaaaga agaacgcaaa gctccacaaa tcctttacgc
    10921 aggaaactca atctcatcta cttaccttgc tgacttggta gagtatgttg cagacaaaga
    10981 cttctcagta aacgtaattt ctaaatcagg tacaacaact gaaccagcga ttgctttccg
    11041 tgtctttaaa gaactcttgg ttaagaaata cggtcaagaa gaagctaaca aacgtatcta
    11101 tgcaacaact gaccgccaaa agggtgctgt taaggttgaa gcagacgcta acggttgggg
    11161 aacatttgtt gttccagatg atatcggtgg acgcttctca gtattgacag ccgttggttt
    11221 gcttccaatc gcagcatcag gagctgacat aaaagctctt atggaaggtg cgaatgcagc
    11281 tcgcaaagac tacacttcag acaaaatctc tgaaaacgaa gcttaccaat acgcagctgt
    11341 tcgtaacatc ctttatcgta aaggctatgc aactgagatc ttggtaaact atgagccatc
    11401 acttcaatac ttctcagaat ggtggaaaca attggctggt gaatcagaag gaaaagacca
    11461 aaaaggtatc tacccaactt cagccaactt ctcaactgac ttgcactcac ttggtcaatt
    11521 tatccaagaa ggaactcgta tcatgtttga aacagttgtc cgtgttgaca aacctcgtaa
    11581 aaacgtgctt attcctactt tggaagaaga ccttgacgga cttggttacc ttcaaggaaa
    11641 agacgttgac tttgtaaaca aaaaagcaac tgacggtgtt cttcttgccc acacagatgg
    11701 tgatgtacca aacatgtatg tgactcttcc agagcaagac gctttcactc ttggttacac
    11761 tatctacttc ttcgaattgg caattgccct ttcaggttac ttgaatgcta tcaacccatt
    11821 tgaccaacca ggtgttgaag cttataaacg taacatgttt gcccttcttg gaaaaccagg
    11881 atttgaagaa ttgagcaaag aacttaacgc acgtctataa tagaagaaaa gagtggtttg
    11941 cccactcttt ttactctctt tatccataga aattggactc agccaagact tgtgatataa
    12001 tatagaaagc aaaaaggcag acgcctagat aataggagaa actatgtcaa aagatatccg
    12061 cgtacgttac gcaccaagtc caacaggact actacacata ggaaatgctc gtacagcatt
    12121 gtttaattac ttgtatgcgc gccatcatgg tggaacattt ctcatccgta tcgaagatac
    12181 tgaccgtaaa cgccatgtcg aggatggtga acgttcacaa cttgaaaacc ttcgctggtt
    12241 aggcatggat tgggatgaaa gtccagaatc acatgagaat tatcgccagt ctgagcgttt
    12301 ggacttgtat caaaaataca tcgaccaact attagctgaa ggaaaagcct acaaatctta
    12361 cgttacagaa gaagagttgg cagctgaacg tgaacgtcaa gaagtagctg gcgaaacacc
    12421 acgctacatc aatgaatacc ttggtatgag tgaagaagaa aaagcagctt acatcgcaga
    12481 acgtgaagca gcagggatca tcccaactgt tcgtttggct gtcaatgagt caggtatcta
    12541 caagtggcat gatatggtca aaggcgatat cgaatttgaa ggtggcaata tcggtggtga
    12601 ctgggttatc caaaagaaag acggttaccc aacttacaac tttgccgttg ttatcgatga
    12661 ccacgatatg caaatctctc atgttatccg tggagatgac catattgcta atacaccaaa
    12721 acagcttatg gtctatgaag ctcttggttg ggaagctcca gagttcggtc acatgacctt
    12781 gattatcaac tctgagactg gtaagaagtt atctaaacgt gatactaata cacttcaatt
    12841 tatcgaagat taccgaaaga aaggttacct tccagaagca gtctttaatt ttattgctct
    12901 tcttggttgg aacccaggtg gtgaagatga gattttctct cgtgaagaac tcattaaact
    12961 ttttgatgaa aaccgcctca gcaagtcacc agcagccttt gatcagaaaa aactagactg
    13021 gatgagcaat gattatatca agaatgcaga ccttgaaacc atctttgaaa tggcaaaacc
    13081 attcttagag gaagcaggcc gtttgactga caaggctgaa aaattagttg agctctataa
    13141 accacaaatg aaatcagtag atgagattat cccattgaca gatcttttct tctcagattt
    13201 cccagaattg acagaagcag agcgcgaagt catgacgggt gaaacagttc caacagttct
    13261 tgaagcattc aaagcaaaac ttgaagcgat gacagatgat aaatttgtga cagaaaatat
    13321 cttcccacaa attaaagcag ttcaaaaaga aacaggtatt aaagggaaaa atcttttcat
    13381 gcctattcgt atcgcagttt caggcgaaat gcatgggcca gaattaccag atacaatttt
    13441 cttgcttgga cgtgaaaaat caattcagca tatcgaaaac atgctaaaag aaatctctaa
    13501 ataaagagga tacaataatg gacatctggg aaaagatgta cgaagaagca cagaaactat
    13561 acaatccaca tgaagtatca gactttgttt atgccaatca tgttgtagct gcagtagaag
    13621 cagaagatgg acaaatattt acaggattct gtatggaggg aacctgtggt gttttccatc
    13681 tctgcgcaga acgggcggcc ctcttcaata cgtaccaatt ttcaggacaa actaaggtta
    13741 agaaagtatt agcctttcga gacaaaccac cttatggtgg aagttcagcc atgccttgcg
    13801 gtgcttgtag agaattcctt ttagagctta atgctgaaaa taaagatgca gaattcatga
    13861 tggactataa tataagaaaa acagttaaag tcgcagaact aatcccttat tggtggggag
    13921 aagaacgtgc ttctaagttt aatgagcgat agaagagtca gtataattct ggcttttttt
    13981 actataagtt gaaaaaat
//
LOCUS       DAVBHS010000044        13981 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVBHS010000044 DAVBHS010000000
VERSION     DAVBHS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13981
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.044"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            81..878
                     /locus_tag="VQ651_000932"
     CDS             81..878
                     /locus_tag="VQ651_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HET2753949.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1026..1148
                     /locus_tag="VQ651_000933"
     CDS             1026..1148
                     /locus_tag="VQ651_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET2753950.1"
                     /translation="MTCDFKFETLQLHAGQVVAPATKSRAVPIYQTTSFIFDDT"
     gene            1170..2210
                     /locus_tag="VQ651_000934"
     CDS             1170..2210
                     /locus_tag="VQ651_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HET2753951.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLG
                     NSLINIPDLEKLAEIAHKHQIPLVSDNTFAIPYLINVFSHGVDIAIHSATKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTCDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKDVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            2381..3655
                     /locus_tag="VQ651_000935"
     CDS             2381..3655
                     /locus_tag="VQ651_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HET2753952.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKDFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3665..4543
                     /gene="truB"
                     /locus_tag="VQ651_000936"
     CDS             3665..4543
                     /gene="truB"
                     /locus_tag="VQ651_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET2753953.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEIILGYSTTTEDASGEVVAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4811..5086
                     /locus_tag="VQ651_000937"
     CDS             4811..5086
                     /locus_tag="VQ651_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET2753954.1"
                     /translation="MEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQSVMANLSQGL
                     SLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5194..5832
                     /gene="udk"
                     /locus_tag="VQ651_000938"
     CDS             5194..5832
                     /gene="udk"
                     /locus_tag="VQ651_000938"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET2753955.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5960..7300
                     /locus_tag="VQ651_000939"
     CDS             5960..7300
                     /locus_tag="VQ651_000939"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET2753956.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7278..7490
                     /locus_tag="VQ651_000940"
     CDS             7278..7490
                     /locus_tag="VQ651_000940"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET2753957.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7487..8362
                     /locus_tag="VQ651_000941"
     CDS             7487..8362
                     /locus_tag="VQ651_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET2753958.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLESLQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8355..9170
                     /locus_tag="VQ651_000942"
     CDS             8355..9170
                     /locus_tag="VQ651_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET2753959.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHSVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9163..9594
                     /locus_tag="VQ651_000943"
     CDS             9163..9594
                     /locus_tag="VQ651_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2753960.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9601..11268
                     /gene="recN"
                     /locus_tag="VQ651_000944"
     CDS             9601..11268
                     /gene="recN"
                     /locus_tag="VQ651_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET2753961.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRTIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLDLNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11270..11998
                     /locus_tag="VQ651_000945"
     CDS             11270..11998
                     /locus_tag="VQ651_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HET2753962.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYSRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12077..13900
                     /gene="lepA"
                     /locus_tag="VQ651_000946"
     CDS             12077..13900
                     /gene="lepA"
                     /locus_tag="VQ651_000946"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET2753963.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         4140 a         2549 c         3341 g         3951 t
ORIGIN      
        1 ttttatgaaa tacagaaaac gcttcctaaa acctaaagtt tgtgatataa ttatcaagaa
       61 aaaagattta ggagtttatt atggtttctt cagaatttat ctcaaagatt gaatttgctt
      121 gcaataagaa agaaagtctt tatagtcaaa gcaaatttaa gtatgcgatt cgttcgatgt
      181 tcgcaggtgc atttttaacc ttcagtactg ctgcaggtgc agttggggct gacttgatta
      241 ataaaattgc accaggtagt ggacgcttcc tctttccatt cgtttttgct tggggcttgg
      301 cctacattgt ttttttgaat gccgagttgg tcacttcaaa catgatgttc ttgactgctg
      361 gtagtttctt aaaaaaaatc tcttggagaa aaacagctga gattttacta tactgtacct
      421 tgttcaacct tatcggagcc ttgatagcag ggtggggctt tgctcattcg gcagcctatg
      481 cgaatctgac acacgatagt ttcatctcag gtgttgttga gatgaagtta ggccgctcaa
      541 atgaattggt cttgcttgag gcgattttgg caaatatttt cgtaaatatt gcgattctgt
      601 catttatttt ggtcaaagat ggtggtgcca aactttggct tgtgttgtca gctatttaca
      661 tgtttgtatt cttaacaaac gagcacattg cggcgaactt tgcttctttc gcgattgtga
      721 aattcagtgt tgctgcggat tcaattgcca acttcggtgt tggaaatatg cttcgccact
      781 ggggtgtgac tttcatcgga aactttatcg gaggaggcct cttgatgggt cttccatatg
      841 ccttcctcaa taaaaacgaa gatacttatg tagattaaga aaatgagcac gattgagtcg
      901 tgcttttttc attttcaaaa taaggtaata gctatttctt atatcaaaat atagaaaact
      961 gatatttgta aactataact caaggtgcta caatatcctt aataaaatga tatggaggtc
     1021 accttatgac ttgtgatttt aaatttgaaa ctctacaact acatgctggt caagttgtgg
     1081 ctccagctac taagtctcgt gcagtgccga tttatcaaac aacatctttt atttttgatg
     1141 acacgtagga aggtgctgat ctgtttgcct tgaggaaacc agggaacatt tatactcgta
     1201 tcaccaatcc tacaacagct gcccttgaag gtggtgttga agcgctagca acagcatcag
     1261 gtatgactgc agtgacttat acgattttgg cgcttgccca tgctggtgac catgtagtgg
     1321 ctgcttcgac tatttacggt ggaaccttca atcttttgaa agaacccctt cctcgttatg
     1381 gtatcacaac aacctttgtc gatgttgata atttggagga agtagaagca gctatcaaag
     1441 acaataccaa gcttgtcttg attgaaacct tgggtaactc cttgattaat attccagacc
     1501 tggaaaaact ggcagagatt gctcataaac atcaaatccc acttgtgtca gacaatactt
     1561 ttgcaatacc ttatttgatt aacgtcttct ctcatggcgt tgacattgcc attcactctg
     1621 cgactaagtt tatcggtggg catggtacaa ctattggagg aataattgtc gatagtggtc
     1681 gttttgactg gacggcttca gggaaattcc ctcaatttgt tgacgagggt ccaagctgcc
     1741 acaatttgag ttatacttgt gatgtgggtg cagcagcctt tattatagct gttcgagttc
     1801 aattgcttcg tgatacaggt gcagccttgt caccattcaa tgctttcctc ttgctacaaa
     1861 gacttgaaac ctcttcactt cgtgtggaac gccatgtaca aaatgctgag acaattgttg
     1921 attttcttgt caaccatcct aaggtagaaa aggtaaatta tccaaaactt gcagatagtc
     1981 cttatcatgc cttggctgag aaatacttgc caaaagatgt cggttcaatc tttaccttcc
     2041 atgtcaaagg tggcgaggaa gaagcacgca aggtcattga taatttagaa atcttttctg
     2101 accttgcaaa cgcggcagat gctaaatcgc ttgttgtcca tccagcaaca accactcacg
     2161 gtcaattgtc agaaaaagac ctagaagcag caggtgtcac accaaactaa attcgtttgt
     2221 caatcggtct tgaaaatgta gaagatttga ttgaagactt gcgcttggcc ttggaaaaaa
     2281 tttaaagtaa aagaagataa acagtgggct tcgactcact gtttttgatt ttccctcagg
     2341 catgatataa tggttaaaga agtctagaaa gaggaacgat atgaacgaaa tcaaatgtcc
     2401 caactgtggg gaagtcttta cagtaaatga gagtcagtat gccgaactct tgtcccaagt
     2461 gagaacggca gagtttgata aggaactaca cgataggatg aagcaggaac tggccttggc
     2521 tgagcaaaag gccatgaatg agcaacagac taaactggct cagaaggatc aagaaattgc
     2581 gcaattacag agtcagatcc aaaactttga tacagaaaaa gaattggcca agaaagaggt
     2641 tgaacagaca agccatgagg ctctcttggc taaggacaag gaagtacagc tcttagaaaa
     2701 tcagttggct accttgcgtt tggagcatga aaatcaactg caaaagaccc tttctgacct
     2761 agaaaaagaa cgggatcagg ttaaaaacca actacttttg caggaaaagg aaaatgaatt
     2821 atctttggct tctgttaagc aaaactacga agcccagctc aaggcagcta gtgaacaagt
     2881 cgagttttat aaggatttta aggctcaaca atctacaaaa gcgattgggg aaagcctaga
     2941 acagtatgca gagagtgagt ttaacaaggt tcgtagtttc gcctttccaa atgcttactt
     3001 tgagaaggat aacaaggtct cttcgcgtgg gtctaaaggg gactttatct tccgtgagtg
     3061 tgatgaaaat ggagttgaaa tcatttctat catgtttgag atgaaaaacg aagcagacgg
     3121 aacagagaag aagcacaaga atgcagattt ttacaaggaa ttggacaagg accgtcggga
     3181 gaagaactgt gagtatgccg ttttggtgac catgcttgag gccgataatg actactttaa
     3241 cacagggatt gttgacgtca gccacgagta tgaaaaaatg tatgttgttc gtcctcaatt
     3301 ctttatccag ttgattggtc tcttacgtaa tgcggcgcta aattccctaa aatacaagca
     3361 ggagttggcc ttggttcgcg agcaaaatat tgacattacg cattttgagg aagacttgga
     3421 tgcctttaag ctagcttttg ctaagaacta taattcagct tcgactaact ttggaaaagc
     3481 tattgatgaa atcgacaagg ccatcaaacg catggaagag gttaagaaat tcctgactac
     3541 atctgaaaac caactccgtc tagctaacaa caaattggaa gatgtctctg ttaaaaaatt
     3601 gacccggaaa aatccaacaa tgaaagcgaa gttcgaagca ctgaaggggg agtagaaagc
     3661 aaaaatgaac ggtattatca acttaaaaaa agaagcagga atgacctcgc atgatgcggt
     3721 ttttaaactg cgtaagattt tgggaaccaa gaaaattggt catggtggaa ccttggatcc
     3781 ggatgtggtg ggtgttttgc cgattgcggt tggcaaggcg acacgcatgg tcgagtttat
     3841 gcaggacgag ggtaagatct atgaggggga aatcattctg ggctattcca cgacgactga
     3901 ggatgctagt ggggaagtgg tcgcagaaac ccctgttttg tctcccttgg atgaaaagct
     3961 tgttgatgaa gcgattgcta gcttgactgg gcctattact cagattcccc ctatgtattc
     4021 ggcagttaag gttaatggtc gcaagctcta tgagtatgcg cgtgctggtc aggaagtgga
     4081 gcgtccagaa cgtcaggtga ccatttatca atttgagcga acaagtccga tttcttatga
     4141 tggccaactt gcccgattca cttttcgtgt aaaatgcagt aaagggactt acatccgtac
     4201 tttgtcagtt gatttgggtg aaaagcttgg ttatgcggct catatgtccc atttgactcg
     4261 tactagtgct gctggcttac aattagaaga cgctcttacc ttggaggaaa ttgctgaaaa
     4321 agtagaggct gggcaattag attttctcca tcctttagag attgggacag gtgaccttgt
     4381 caaagttttc ctaactccag aagaggctac agaagttcgc tttggtcgtt ttattgagct
     4441 agaccaaacg gacaaagaac tggctgcctt tgaagatgat aaattgttag ccattctaga
     4501 aaaacggggc aatctctata agccaaggaa ggtttttagc tagatcgttt aggaataaaa
     4561 atcgggtgat agataacaat tgcttgataa acccccatac taatagtaga atggttttgg
     4621 gaattataat attccaattg ttgcgagttg tagatactca aataatctat atagaaattt
     4681 agaggtgtga aatgaagcaa cttaaaattc tttcagataa atatttagag tccatgacag
     4741 gttctggtgg gaacttaggt ctaggatttg gtgtgataat tccatgatgt gaaatgagtt
     4801 tcgagaaagg gtggagcaac ttcttcaaca aaaagaaata aatgaaaata gtgagttgag
     4861 tcacctgttt cgtcttgcta tacaaaattt agacagaaat gaaaaatacc aatcggtcat
     4921 ggccaatttg agtcaagggt tgtcacttta cctcatgacg catcattacc aggcacctaa
     4981 gtctgtcatt gattttggtt tatggattgc caaagctcct agccaggaaa gagggagact
     5041 ggctttcttg cagatgcttg ctcaaaccct acagggcttt cgttaaaact gtcgaacatc
     5101 agagggaaga ctctggtgtt ttttgctgat gagactataa attttgtaaa aaatgtgata
     5161 gaatagacga cggataaaat aacggaggat agcatgcaaa atagaccaat cattattgga
     5221 gtgacaggtg gttctggtgg tggtaaaacc agtgtttcaa gagccatttt atcgcatttt
     5281 cctgatgaaa agatttccat gattgagcat gattcatact acaaggatca gtctcatttg
     5341 acctttgaag agcgtgtcaa aaccaactat gaccatcctt ttgcctttga tacagacttg
     5401 atgatcgagc agattaagga attgttggca gggcgtccgg tggacatccc gacctacgac
     5461 tatacagagc atacacggag tagcaagacc tatcgtcagg agcctcaaga tgtctttatc
     5521 gttgagggca ttttggtctt ggaggacaag cgtctgcgcg atttgatgga tatcaagatt
     5581 tttgtggata cggatgacga tgtgcgtatt attcgtcgta tcaagcgtga tatggaggaa
     5641 cgtggccgta gccttgatag cgttattaac cagtacttag gtgtggtcaa accaatgtac
     5701 caccagttta tcgagtcaac taagcgttat gctgatatcg tcattcctga aggggttagc
     5761 aataccgtgg ctatcgacct gttgacgacc aagattgcaa agattttgga agaagctcga
     5821 aacagcaaat aatcacatgt ggaggccttg cctccttttt ctatttttcc ctcataatgg
     5881 tacataaatg gagattttta ggcatatttt tggtataata atacccataa aagagcaagt
     5941 aaatgaatag taggtggaga tggaaaagta tttatcggta acaactttga ccaaatatct
     6001 gaaaatgaaa ttcgataaag acccatactt ggaacgggtc tatttaactg gtcaagtttc
     6061 caactttcgt aaacgaccta ctcaccaata tttctcccta aaggatgacc atgcagttat
     6121 tcaagcgacc atctggtctg ggatttatca gaaattaggg tttgacctgg aagaaggaat
     6181 gaagatcaat gtgattgggc gtgtacaggt ctatgaacca agtggtagct actccatcat
     6241 cattgaaaaa gctgagcctg atggggttgg ggcgcttgcg attcagtttg aacaacttaa
     6301 gaaaaaattg acagaagaag gcctgtttca agaacgcttc aagcaagctc taccccaatt
     6361 ttctaagaga attggggtag taaccagccg tagtggagcc gttattcgag atattatcac
     6421 gaccgtcagc aggcgattcc caggtgttga catccttctt tatcctacca aggttcaagg
     6481 tgaaggggct gcggaggaaa ttgctagaaa tattgcgcgt gctaatcaac gggacgattt
     6541 ggatttgctc attattggtc gtggaggtgg ttctattgag gatctctggg cctttaacga
     6601 agaaattgtg gtacgagcta tttttgaatc tcgtttgcca gttatctcta gtgtggggca
     6661 tgagacggat gtgaccttgg cagattttgt ggcagatcga cgcgctgcaa cgccaacagc
     6721 ggcggctgaa ctggcaacac ctgtgaccaa gttggatgta ttagctcatt tgcaaaatca
     6781 ggaaaaacgg atggcaacgg cagtccgaaa tgttctatct aagaaacaag aggctttgaa
     6841 aaaatgcagt cagtctgtta tctttagaca acctgagcgc ttgtatgacg gttatttgca
     6901 acgcttggac caactgcaac tgcgtttgaa acaaagtttg cgaactcgga tttctgataa
     6961 caaacaatta gttcaagcaa gaactcatca attagtacaa ttatcacctg ttaccaaaat
     7021 ccaacgctat caagaccgtt taggacagtt ggacaagctc ttaggtagcc aaatggcgtt
     7081 agtttatgac gccaaggttg ctgaggccaa gcgactttcg gaagctttgc tcatgttgga
     7141 tactagccga atcgtggcgc gtggttatgc tattgtcaaa aaagaagagt ccgttgtaga
     7201 ttcggttgag agtttgaaga aaaaagacca agtaacgctt ttgatgcgag atggtcaagt
     7261 agaattagag gttaaagatg tcaaaacaaa agaaatttga ggaaaatcta gcagaactgg
     7321 aaaccattgt ccaaagtttg gaaaatggtg aaattgctct ggaagatgcg attactgcct
     7381 ttcaaaaggg catggtcttg tcaaaagagc tccaagctac gctggacaag gctgaaaaga
     7441 ccttggtcaa ggtcatgcaa gaagacggaa cagaaagtga ttttgaatga aaaagcaaga
     7501 aaaattagct cttgtcgagt cggctttgga agatttttat ggagaccagc agtttgcctc
     7561 tagtttacgg gagtctgttc tctattctat tcatgctggt ggcaagcgta ttcggccttt
     7621 tctcttgtta gaagttctgg aatccttgca ggttaccatc aaacctgctc acgcgcaggt
     7681 agctactgcc ttggagatga ttcatacagg gagcttgatt cacgatgacc ttcctgctat
     7741 ggatgatgac gattatcgaa gagggcggtt aaccaatcac aagaaattcg gtgaagctat
     7801 ggccattttg gctggagatg ccttattctt agacccatat gccttgattg cgcaggcaga
     7861 tttgccaagt cagatcaagg tggacttgat tgccaactta tcccttgctt caggtagtct
     7921 gggtatggtg gcagggcaag ttttggatat ggagggcgaa caccagcact tgtctctgga
     7981 agaacttcag actattcatg ccaataagac tgggaagtta ctagcctatc ccttccaagc
     8041 ggcagctatt atagctgaat tgtcacctga aatgcaggtg aagctgaaaa ctgtgggtga
     8101 attgattgga cttgcttttc aagtcagaga tgatgtactg gatgtgacag ctagttttga
     8161 ggaaatcggc aagacacctc aaaaggatct gcaggcagaa aaatcaacct atcctgcctt
     8221 gttgggcttg gaagagtcca ttgccttttg taaccaaacc ctggatcaag ctaatgaaaa
     8281 attagaagaa attgcccagc agcttccctt tgaaacagaa tcgattgtaa gtgtagtaga
     8341 aagtttgaga atcaatggct aaggaaagag tggatgtact agcttataaa caggggttgt
     8401 ttgaaacgag agagcaggcc aagcgaggtg tgatggctgg tctagtcgta gcagtcctta
     8461 atggagaacg gtttgacaag ccaggagaga aaattccaga tgacaccgaa ttaaaactca
     8521 agggggagaa actcaagtat gtcagccgtg gtggtttgaa actggaaaag gccttgcagg
     8581 tctttgattt gtcggtggat ggcgcgacta cgattgatat cggggcctct actggaggtt
     8641 ttaccgatgt catgctacag aatagtgcca agttggtctt tgcagtcgat gttggtacca
     8701 atcagttggc ttggaaatta cgccaagacc cacgagttgt cagcatggag cagttcaatt
     8761 ttcgctatgc tgaaaagact gatttcgagc aggagccgag ctttgccagt attgatgtga
     8821 gtttcatttc ccttagtctg attttgccag ccttgcacag tgtcttggct gatcaaggtc
     8881 aggtggtagc acttgtcaaa cctcagtttg aggcaggacg tgagcagatt gggaaaaatg
     8941 gaattattcg agatgctaag attcatcaga atgtccttga atctgtaaca gctatggcag
     9001 tagaggcagg tttttcagtc cttggcttgg acttttctcc catccaaggt ggacatggaa
     9061 atattgaatt tttagcgtat ttgaaaaaag aaaagtcagc aagcaatcag attcttgctg
     9121 agattaaaga agcagtagag agggcgcata gtcaatttaa aaatgaataa aaaagaaaga
     9181 cttgaaaaaa ttagaagatt ggtgacagat tatcaaatcg gcacgcaaga agaaattgta
     9241 gaacatttga aagaagcagg tatcactgcc actcaggcga cggtatcccg agatatcaaa
     9301 gagttaggta ttgtcaaaat tcctttgaga gacaacacct atgtctacga attgccaaaa
     9361 tcaatcgtaa aaagtctgca actggctgaa gacaatatcg aatcggctga attgatggat
     9421 aagatgatca atctccaagt tattccagga aatacggctt ttgtaaaagc tcagttaatc
     9481 gagacttttg cagacaagat ttttagctgt ttgactgatg atagctcgat tttagtcatt
     9541 gccagaagtg gaagtctagc agaggaaatc tttgaacaag taaaaaattg gtaggtctat
     9601 atgttacttg aaatttcgat aaaaaacttt gccattattg aggctatttc cctcaatttt
     9661 gaaaagggga tgactgtcct gactggtgaa acgggtgcag ggaagtcgat tatcattgat
     9721 gccatgaata tgatgttggg agctcgtgcg acgacagatg ttattcgtca tggtgcacca
     9781 aaggcagaga ttgaggggct tttctcagtt gagaatagtc gtcttttaca ggaaattttt
     9841 gatgagcaag gtttagaatt aggtgatgaa attatcatcc gtcgggaaat cttgcaaaat
     9901 ggtcgtagta tcagccgtgt aaatggtcag atggttaatc tgtctgtttt gcgaactatt
     9961 gggcaacatc ttgtagatat tcatggtcag catgaccatg aggagttaat gcgtccccaa
    10021 ctgcatatcc agatgttgga tgaatttggt gatgccgctt tttgggactt gaaagaaacc
    10081 tatcaaacga gttttgatgc ctatcggaaa atgcgcaagc aggttctgga agtcaagaaa
    10141 aaccaacagg aacacaaggc acgtatcgaa atgttggaat ttcaaatggc agagattgag
    10201 gcagcaaact tgcaggctgg agaagacttg gatctcaatc aagagcgaga taaactcctc
    10261 aaccacaaaa atattgcgga tacactgacc aatgcctaca gtatgttgga caatgaagat
    10321 ttttcaagtc tggccaatgt tcgttcagcc atgaatgaca tggaaagtgt cgaagagtat
    10381 gaccctgaat accgtgaaat ttcaagctct ctgtctgaga cctactatgt tttagaagac
    10441 attagcaaac gtttggaagc tatcattgag gaccttgatt ttgatggcaa tcgcctcatg
    10501 caggttgaga atcgtttgga cctccttcat actattactc gtaagtatgg tgggactgtt
    10561 gatgatgttt tgctttattt tgccaagatt acggaagaat acaatctctt gacaggcaat
    10621 aatctttcgt ctgaggacat ggaagcagag cttaagaagt tggaagtcaa tcttgtcaat
    10681 ttggcaggtc aacttgcttc tgctcgtcat aatttggctc agcaactcga agctgagatt
    10741 aaacaagaac tgcaagatct ttatatggaa aaagcccagt ttcaggttcg ttttagtaag
    10801 ggaaaattca gtcgtgaggg aaataaaatg gttgagtttt atatttcaac caaccctgga
    10861 gaagacttta aacccttggt taaggttgct tctggagggg aattatctcg tctcatgtta
    10921 gccattaagt ctgccttttc acgtaaagaa ggcaagacta gcattgtctt tgatgaggtg
    10981 gatacgggag tttcaggtcg tgttgctcaa gctattgctc agaaaattca taaaattggt
    11041 cagcatggtc aggttttggc tatctcccat ttgccacaag taattgcgat tgcagattat
    11101 caattcttta ttgagaagat tagtaatgac cattcaacgg tttcgactgt tcgtctcttg
    11161 acggtcgaag agcgagtgga ggaagttgcc aagatgttgg caggtgatga tgtgacagaa
    11221 gcagccctga cgcaagcaag agaattgttg agaaacaggg agaaataaga tgacagatta
    11281 ttatgtaatt ggagatgttc acggaaaagc tgggatgctg gaagaccttc tcaaaacctg
    11341 ggatggtcag ccccagttgc tctttctagg tgatttgatt gacagaggtg aggatagtca
    11401 ccgtgttctt gaaatggtca aggacttggt ggacaatcaa ggggctatct gtttgtccgg
    11461 aaaccacgag tatatgtttt tgacttggct agatgaccca gaagaaagtt atgaccatta
    11521 tagtcgcaat ggtggagata caaccattaa ctctatccta ggtcgtccct tggatgcacc
    11581 agttgatgga gtagaagatg ccaagcgggt tgccgctgaa gcggcagact tggtcgaatt
    11641 cattcgtcaa atgccatttg tagtagagac agacaagtat atctttgttc acgcaggtat
    11701 tgatttgacc ttggacgact ggcatgaaac aacagattat aaaaaagtat ggcttagaaa
    11761 accattccac ggagctgaaa atcatactgg aaaaaccatt gtcttcggtc atacaccggt
    11821 ctatggttta ttacagcaag aacgcggtac atctgagctt tggactacag aagatggcaa
    11881 gattggaatg gatggaggag ctgtttatgg tggtgtcctt cacgggattg tctttacaga
    11941 ccaaggaatg acagaacacc actttatcga aaatgacggc tttgtcgctg aagattagta
    12001 ctcctagcag ggtatggtct tgtcaaaata tcaaaaacaa tttataataa atagataccc
    12061 tgaaaggaag agaatcatga acttagaaga attgaaaaaa cgacaggaga agatccgtaa
    12121 cttctctatt atcgcccata ttgaccacgg aaaatcaact ctagcagacc gcattttgga
    12181 aaaaacagag acggtttcaa gtcgtgaaat gcaggcccag cttttggata gcatggagct
    12241 agagcgggaa cgtggaatta ctattaagtt gaatgccatc gagttgaatt acactgcaaa
    12301 agatggggaa acttatattt tccacttgat tgacacacca gggcacgttg actttaccta
    12361 tgaagtttca cgttcgctag ctgcctgtga gggtgctatt ttggtggtcg atgcggctca
    12421 aggaattgag gctcaaactc ttgccaacgt ttatctggct ttggataatg atttggaaat
    12481 catgcctatc attaataaaa ttgacctgcc ggctgcagat ccggagcgcg tgcgtacaga
    12541 gattgaagat gtgattggtt tggatgccag tgaagcagtt ttggcttctg ccaaggctgg
    12601 tattgggatt gaagaaatcc tcgagcaaat tgtagaaaaa gtaccagcac caacgggtga
    12661 tgtgacggcg ccacttaagg ccttgatttt cgactctgtt tacgatgctt accgtggggt
    12721 tatcctccaa gtgcgtgtca tggacggagt ggtcaaacct ggtgataaga ttcagctcat
    12781 gagcaatagt aagacctttg atgtggccga agtcggtatt tttacaccaa aagcggttgg
    12841 tcgtgatttc cttgcgactg gtgatgttgg ttacattgcg gcgtctatca agacggttca
    12901 ggatactcgt gtgggtgata ccgttacctt ggcaaccaat cctgcggcag aaccattaca
    12961 tggttataag cagatgaatc ctatggtctt tgcgggtctc tacccaatcg aatcaaacaa
    13021 gtacaatgac ctgcgtgaag cgcttgaaaa attgcaactg aatgatgcta gtcttcagtt
    13081 tgaaccagaa acatctcagg cacttggatt tggtttccgt tgtggatttc ttggacttct
    13141 ccatatggat gttatccagg aacgtttaga gcgtgagttc aatattgacc tcatcatgac
    13201 agctccgtct gttatttaca aagttaattt gaccgacggt gagtctatgg atgtgtctaa
    13261 cccatctgag tttccagacc caactaagat tgcgaccatt gaagagcctt atgtcaaggc
    13321 gcaaatcatg gtaccacagg agttcgtcgg agcagtaatg gagctagctc agcgtaagcg
    13381 tggggacttt gtgactatgg actatattga tgataaccgt gtcaatgtta tctatcaaat
    13441 tcctcttgct gaaattgtct ttgacttctt tgataaactt aagtcttcga cacgtggtta
    13501 tgcaagcttt gactacgaat tgtcagaata tcgcccatct aagctggtga aaatggatat
    13561 tcttctcaat ggagataagg tggatgccct cagctttatc gttcacaagg actttgccta
    13621 cgaacgtggg aaactcatcg ttgataaact caagaaaatc atccctcgcc aacaatttga
    13681 agttccaatc caagcggcta ttggacacaa gattgtcgct cgtactgata tcaaggccct
    13741 tcgtaagaac gtacttgcta aatgttatgg tggtgacgtt tctcgtaagc gtaaactcct
    13801 tgaaaaacaa aaagctggta agaaacgcat gaaatccatc ggatcagttg aagttccgca
    13861 agaagccttc ctcagcgtct tgagtatgga tgaagaataa gttatcagaa atgcctttcc
    13921 ttgttcacag gaaatttata agaatcaata gtaaagcagg cttagatggt ctgctttttt
    13981 a
//
LOCUS       DAVBHS010000045        13793 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVBHS010000045 DAVBHS010000000
VERSION     DAVBHS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13793
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.045"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            131..607
                     /locus_tag="VQ651_000947"
     CDS             131..607
                     /locus_tag="VQ651_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2753964.1"
                     /translation="MKLSLYSEVEHYHFLSSYSLNDITYTDTPKNIIEKSKKDKYRYP
                     IVILNKDHKVIGFFCLHLNTGPKEYGYYQNDYALIRGFSIHDNFRNQGYGSNALNEIF
                     EFIDSTLKLEINHIILAVNEKNILAQKAYRNSGFSVVKKDLEGKRGKLIIMEKSRN"
     gene            complement(1281..1673)
                     /locus_tag="VQ651_000948"
     CDS             complement(1281..1673)
                     /locus_tag="VQ651_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET2753965.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLTRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTDKSQYEFLFPSVK
                     IKEEKTDKKEEVATDSSEKAEKKIRREA"
     gene            complement(1651..2112)
                     /locus_tag="VQ651_000949"
     CDS             complement(1651..2112)
                     /locus_tag="VQ651_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET2753966.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVGQLEAELDRSQFEKVEGNRLYMKQDDKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            complement(2075..2335)
                     /locus_tag="VQ651_000950"
     CDS             complement(2075..2335)
                     /locus_tag="VQ651_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753967.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            complement(2340..2768)
                     /locus_tag="VQ651_000951"
     CDS             complement(2340..2768)
                     /locus_tag="VQ651_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET2753968.1"
                     /translation="MEGQIVKSMIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVE
                     EQIFFMEFEELYRETQKRSVASQQKTSLNLDGQMISNGSQKLTVPKGIQAPSGQSITF
                     DRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            complement(2737..3063)
                     /locus_tag="VQ651_000952"
     CDS             complement(2737..3063)
                     /locus_tag="VQ651_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET2753969.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            complement(3065..4081)
                     /locus_tag="VQ651_000953"
     CDS             complement(3065..4081)
                     /locus_tag="VQ651_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET2753970.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLDFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            complement(4029..4970)
                     /locus_tag="VQ651_000954"
     CDS             complement(4029..4970)
                     /locus_tag="VQ651_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HET2753971.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            complement(5046..5411)
                     /locus_tag="VQ651_000955"
     CDS             complement(5046..5411)
                     /locus_tag="VQ651_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HET2753972.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            complement(5562..6620)
                     /locus_tag="VQ651_000956"
     CDS             complement(5562..6620)
                     /locus_tag="VQ651_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET2753973.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            complement(6783..7934)
                     /gene="nagA"
                     /locus_tag="VQ651_000957"
     CDS             complement(6783..7934)
                     /gene="nagA"
                     /locus_tag="VQ651_000957"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET2753974.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            complement(8087..9904)
                     /locus_tag="VQ651_000958"
     CDS             complement(8087..9904)
                     /locus_tag="VQ651_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HET2753975.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMIFLLSATAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            complement(10005..11147)
                     /gene="tgt"
                     /locus_tag="VQ651_000959"
     CDS             complement(10005..11147)
                     /gene="tgt"
                     /locus_tag="VQ651_000959"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET2753976.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMLLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAECGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFRKYFVEKYGYNKSGRNF"
     gene            11277..12134
                     /locus_tag="VQ651_000960"
     CDS             11277..12134
                     /locus_tag="VQ651_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HET2753977.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            complement(12162..12806)
                     /gene="pcp"
                     /locus_tag="VQ651_000961"
     CDS             complement(12162..12806)
                     /gene="pcp"
                     /locus_tag="VQ651_000961"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET2753978.1"
                     /translation="MKVLVTGFEPFGGEKGNPTLEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSVTPERVAINQDDARISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRPTTPTMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(12913..13281)
                     /locus_tag="VQ651_000962"
     CDS             complement(12913..13281)
                     /locus_tag="VQ651_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HET2753979.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATRSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLWYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            complement(13292..13717)
                     /locus_tag="VQ651_000963"
     CDS             complement(13292..13717)
                     /locus_tag="VQ651_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET2753980.1"
                     /translation="MIEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIKVKEEMESILTEFERTELSRLLNKLVLGIENIEI"
BASE COUNT         4097 a         3096 c         2418 g         4182 t
ORIGIN      
        1 tgaaaataaa ggactaaagc aataactcta aatacatctg atttttatag gatatttcat
       61 aaaaaaaatc tacataccta gagtcctatt tagttatgta tcttacataa agagaaagga
      121 attaattatc atgaaattat ctttgtatag tgaagttgaa cattaccatt ttttaagttc
      181 ttattcttta aatgatatta catatactga taccccaaaa aatattattg aaaagtctaa
      241 aaaagataag tatagatacc caatagtaat acttaataaa gaccataaag taattggatt
      301 tttttgccta cacctaaaca ctggtcctaa agagtatggc tattatcaaa atgattatgc
      361 gcttattaga ggattttcta ttcatgataa tttcagaaat caaggatatg gttctaatgc
      421 tctgaatgaa atatttgaat tcatagactc aactttaaaa ttagaaatta accatattat
      481 attggctgta aatgaaaaaa atatattggc tcaaaaagca tatagaaatt ccggtttttc
      541 tgttgtgaaa aaagatttag aaggaaaaag ggggaaatta attattatgg agaaaagcag
      601 aaactagtaa tgatactagt ttctgctttt ctctaagtct gctataaatt tataacatgt
      661 tgaaataaga tgtgaataaa ttgattgcag aattcaaacc aatttctaac aatgttttag
      721 aaaactcggc ggactattcc agattcaatc cactatattc tatcttattt gcatattttg
      781 agagaaataa tctatatact aaacttttat gtttataatt cattcctttc gtttcactca
      841 aggcacaaga cagaatgaaa aagtggtgtg ctctttattt tgttttataa taatagtgag
      901 aaaacctatc actactacaa atcacgggga ggtgaataag tgagtggtat agccactacc
      961 tcgcattttt ataggtgtca ttctttccat caagcacaag aactgtgact aggagcacgt
     1021 aaacttataa ttgaataagc gactcgtttg tgaaaggaaa gaatagtcaa gggataggca
     1081 gactcaaatt tggtgaaggg aaacccctca tgttaaaagc caaacttcaa ttttaaaaat
     1141 tttaaaattt gtttcgtatt cactactcct tgcctttata ttggattgat taagagaacc
     1201 tttcgcgttt ctagaaacta cagtggacta ttctggattc aacgcattat agttgaattg
     1261 actaggaatt ctctttcttt tcaggcttct cttctgattt ttttctccgc tttttcgctt
     1321 gaatcagtcg ctacctcttc ctttttatcc gttttctctt ctttgatttt gactgaagga
     1381 aacagaaact catattgact cttatccgta cgaacagtcg tcaccaagct tgttttcttg
     1441 ttttgatagc tcacctgacc tagattaaaa acctgttccc cactttcttg ctcggcttta
     1501 tccttggttc gtttagccat ggcaaaagca accaattttt ctttatttaa agcatagtct
     1561 tgatagtggg cgatttgtcg ggtcaaataa aattgcaaca aaagactaaa gatggctgct
     1621 atggtgactg cgtagaggag aacacctgcc ttaactttct tctttttcca cacgatagat
     1681 gaactccctt tctaagcctt tttgaaactg gaaacgaaaa tgaaccagtt gattgtcctc
     1741 tgtaatctga gctgatttga ggccataaac cataggctga tacccccgtc cactagcatc
     1801 ggttttacga aaatcgtctg actttgactt accgatggcg atgtccttgt catcttgctt
     1861 catgtatagg cgattgcctt ctactttttc gaactgcgaa cggtctaatt ctgcctcaag
     1921 ttggcccaca aacaagagcc actccttttg ctcgctttgt tgctggtagc gaacttctga
     1981 aatgaggagc tgactcatag cttgaaaaag gagtaatccc ccactgatga caatgagggc
     2041 aagcagggat tctaacaagg taaaagcctt gaccttatgg ctctttgatt gccaacaact
     2101 gttctgaacc atggtagacc tccaatccct tttcactaga aaatacctga atctcaactc
     2161 cgttgatgct tacctgattt tgccccgtct gcaaggccat cttagctacc ctcaagactt
     2221 cttccttttg caagattttt gcttcctctt gcctattttt ttgaatttgt cccaaaagga
     2281 gggtcgcaat gctggcaaag atagctagag cgactactgc ttccagtaaa atcactgccc
     2341 taattttttg tttccttaat gcgtttaatt tttccatttc ctagatataa ttgatagcga
     2401 atcgctcctt tactggtctg aaattcaacc ttagccaggg acgaattgcc cccagctcgg
     2461 tcaaatgtaa tactttggcc tgatggtgcc tgaattcctt taggaactgt caacttttga
     2521 ctgccattgc taatcatctg cccatccaag ttcagactag tcttttgttg actagctaca
     2581 ctgcgttttt gggtttcccg atagagttct tcaaactcca taaagaaaat ctgttcctct
     2641 accgctgcaa aagtggactg gacagagccg gacaagccca aggcaaggat actcacaagt
     2701 cccaaaacca agagactttc cagcatggta aaggccttaa tcattgactt tacgatttgc
     2761 ccctccattt ttagtatagt attcattata agctttaacc tgttcttccg tgatgcgccc
     2821 atctccttgt aacttgctca gagtagcatc ttcattttta tccaagctat aaagttctgc
     2881 ctggctttcc accaccttaa caacagctgc ttttcctttg tcattgactg cttctttttg
     2941 cttggtcaga ttaggtacaa agagcaagaa aagcacgctg ataatcagca agaccaccaa
     3001 catctccacc aatgtaaaag ctttaacctt agcttttttc aagaatgtca tcattttttt
     3061 cattttaaaa atttacctcc atattttgat acatgggcat gagcattgcc gcataaagta
     3121 aaacgataat cagtgccaca aagataaaaa ccagtggctg caccaaattc atggtgcggt
     3181 tgactcgggt aaaaaaggct tcccaagttt tttcagcata gatttccaac tcactaccca
     3241 gcttggactt aacttcccca tactctatga tgagactcaa ttccttccta aagaaaggat
     3301 aggttcctat cgtctgagaa aattcacggc catttttcag ggtttgagcc agatcttgac
     3361 cgatttcttt aaagagctgg gaaccttgtt cctgcatcat ttgaaaaatc tgcgtcagct
     3421 ccattccctg tgaaatcata ttcccccatt cacgtgcata ataggctgtc aagtaggtct
     3481 gcacaaagat tccaataaag ggaaggcgtg ctaagataga aaagacactc atcttagaac
     3541 ttcttttata aaaagttagt gctaaaaggg caagcacgga aacaagccct accatgccta
     3601 gaaaaatttg tggcagatta ccgataattc gggtggcaat attgctacta tccagttgtg
     3661 ggagcaggta attccgtagc cccagcataa ttaagagaag aaaatccagc aaaatcaaag
     3721 gataggtcgc tacttcaatt aactttttct tgaccttggc cagattgtct agatattctt
     3781 ctatctttcc caaactcagg tgaagattgc catgaacttc agctagagat aactgagtga
     3841 caatggtact tgaacatccc aaactttcca tcatttctga gaatgatttc ccctgagaca
     3901 agcctgtacg catctgggtc acacactgct tgtccaacaa ggaactccta tctaaaaagg
     3961 agatagtctc caccagatga aaaccgctag aaaagagatt gttaaatagg gtgatgatat
     4021 ttttttgctt agctgtagct aattttttcc gtctcagcct gaagacttgt gatatgtcca
     4081 tctttaagaa gttggtcaat ttgctcattc cacttggctg cttggtgttc ttgataatct
     4141 ctgtttgcaa agtcaacgat tcctcctccc ccgattaatc tctggtagca gactccttgc
     4201 agaacaactg ccaattcttc ttcactcaca cccaactcca gcagacgctc ataaacacct
     4261 cggatactct tggcgtgaat ggttgaaaag actgtcgcac ctgtcaaact agctctgacc
     4321 actgcacgcg ccgtctcgct gtcacgaatt tctccgataa tcaagagatc tggtcgatga
     4381 cgcaaggaaa gtttgattag attttcatag gttaggccga ttgcttcgtt caactgcaac
     4441 tgaagcatgt cgtcctgctt gatttcgaca ggatcttcga tggacataac ttgctgtcct
     4501 ttaaagagtg acttggacaa ttcatgcatc aaggtcgtct taccactccc aaccgggcca
     4561 gcaaaaagat agagtccccg ttgcctgtac tgcttgccta attcttcaat atcctgaaac
     4621 caaaaatgca agtcctgctc ctcatcgtgc aacaaacgga taaccaaact ctcatgcccc
     4681 cgataatcgc ctacagtaga taaacgtaga gacgccatct tctggtcata ggcataatca
     4741 caggaaccca gttgactacg tcttttttct cccacattca tacccgccac aaacttaaag
     4801 tgactgataa cggctgcaaa cttttcaaaa tcatagctac caattttaca gcgctcgtct
     4861 cctaccctca tatgaagctc ataggcgtct aacttaggga caaaataaat atcctgcgtc
     4921 ccttttttcc gagctgaacg aatgatttct tgtgcaattt cttgaaccat acttacctcc
     4981 tcacctatac tattcgcaaa gcgttagaaa aataaaaaaa gcaactccaa aaaagttact
     5041 tttctctatt ttaatttcat acggcaagaa cgattccttt cctgactggt ttgtttttca
     5101 tagcctgagc gtagtttttc gtctgggata acctgctcat cctgcaaaag agccaaagaa
     5161 tggtattgtt gtaaatactc atcacattca tcacaccagc gttggtcttc cgggtcccaa
     5221 aaaatggtca tcaagtcgct tctactttta taaagagggg attcttgttc caatctaaac
     5281 cagcaagttt tgtagtattt gagagcatca taatactggt caaattttct acttgctaca
     5341 atatcttctt cccaaccttc taagaaccac cacggttcaa aatctccgta catttctata
     5401 acacgataca tattcattca ttcctttgta ccgtatatta taaccaattc cgccagacaa
     5461 ggaaagaaat atgctcattt cttaattttt tgataaaaaa tccagccatc tgatggatga
     5521 ctgaatttct atttgcttat gtacaaaaat atttttacta cttaggcttc tgagatatcg
     5581 ttttcgataa tgaccttaat ggcatggtgg tctgctgcct tactgaagac ttcgtaggct
     5641 ttttcaattt cactgagttt gaaatagtga gttaccaatt tttccggttc aatcttatga
     5701 ctttcaagtg ctttcaacaa ttgtggagtc gtatttgtag ataccaaacc agttgttaca
     5761 ttgatgttgc gaatccaaag tttatctaaa tcgaattcaa ctggtttacc atgcacacca
     5821 cagttggcaa ccgttccgtc tacaccgata atcttttgac agaaatcaaa tgtttcagga
     5881 atccctacag cttcgatagc gacatccaca ccacgaccat ctgtcaaatc ataaatttct
     5941 ttaatggctt ttttagggtc tgaagaatta accttatgag tcgcaccgaa tgatagggca
     6001 gtttccaagc ggttatcgtc taggtctacc ataatcaatt tagctggtga atagaattgg
     6061 gctgttaaaa gagcagccaa tccaactgga cctgaaccaa taatggctac gctgcaacca
     6121 ggttctactt tcccttttaa gacaccaatt tcatatccag taggcagaat gtctgacagc
     6181 ataaccaaag cttcatctga caagtcttct ggagtatggt aaagagtatt atctgcatga
     6241 gggacacgta gatattcagc ctgcatacca tcaatcaagt gaccgaaaat ccagccccct
     6301 tcgtcttcac agtgggcata aattcctttt ttacagtagt agcatttacc acaggcacag
     6361 acgcaagaaa tcaagacctt gtcacctttt ttgaagttgg aaactccttc cccaacttct
     6421 tcaacaatcc caatcccttc gtggccaaga atggtaccac tttggcaagt aggaacatcc
     6481 cctttgataa tatggaggtc tgttccacaa atagtggttt ttacaatacg cacaatagcg
     6541 tctgttggct tgcgaataac tggtttgtct acatcaacaa aagaagcaag tcctggttta
     6601 acataagtat aggctttcat aaagcactcc tccgtttttt tatttgtaat atttatagtt
     6661 caattgtaag cctttttctt tgtttcttca agcttttttt gtgatttttt taacttttta
     6721 tttaccagta aagtaccctg tataaaaaag cagaagctag tctctaactt ctgccttctc
     6781 tcttatgctt gataacgttt cacaccatct aggtaggttg ctaccaattc caaatcttta
     6841 tctaatacga tgaagtcagc gtcgtagcct tcacggattt ggccacagac gtcatcgatg
     6901 tgaacggatt tagctgggtt gaagctggcc atcatgactg cttcatgcgg attcgcaatt
     6961 ccccattcga ccacattctt caaaccatct ttgagtttga ggatagaacc tgccaagtta
     7021 cctgtcgatt tgaggcgtgc agttccattt gcaacgacaa ctgggaattc tcccaacata
     7081 taatctccgt cttccaatcc cccagctgtc atacagtctg tgataagagc gatgttttct
     7141 gttccttttt gtttgataag aatttcgcaa gcctttggat ctacgtggtg accatcacag
     7201 atcaactctg cataggtatg tggcaattgg tacatggctc caaccatacc caattcacgg
     7261 tgagtcaacc cacgcattcc attgtaggca tgcacccaaa cactcgctcc agcatcgact
     7321 gcttttttgg cttcatcaaa agtcgcgttt gaatgtccaa gagcaaccgt cacaccttcg
     7381 cccgtaactg tacgaacaaa gtcttccacc ccatcacgtt ctggtgcaag ggcaatttta
     7441 ttaagcaagc catttgccgc tttttgccaa gaatgaaact cctcaacacc cgggtctctc
     7501 atataagttg gattttgtgc ccccttaaaa gtttctgtga aatatggacc ttcataataa
     7561 atcccacgaa tcttagcacc tgttgcttct ttataatggt ttccaagatt ttcagtgact
     7621 gcaagcaatt gctcataagt ggctgttaaa gttgtgggta agaaactggt aacaccggta
     7681 ctaagaagtc cttcactcat agtatgcaat gtaccttcaa tgttgttgtc catcacatct
     7741 acacctgcat atccatgaat atgagtatcc acaagtcctg gggcaatgct ataacctgta
     7801 tagtcaatca cctcagcccc ttcaggaatc tgctctacat gtttcccaaa cttgccgtcc
     7861 acaagttcca agtaaccacc tcgacgaact ccgtgtgggt agaaaaactg atccgcttta
     7921 atatagttag gcataatgtt aacctcctta aaagattgat tctacaattt attatgtcaa
     7981 ttacattata aacctttctt ttttatttgt aaatggtata gacctatttt ttggagatat
     8041 tttaaaagag ctggatttct ccaactcttc tcttttttaa tacaagttat tttgatttaa
     8101 ccggcttgtc ttgagctgtc tgcaaagctg tggcaatcgt atctgcatac aattttgctc
     8161 ctgcttcgat agtgctactc tcactcccga aatgaacctg gtctgttcca gcccaaattt
     8221 ctggatgctc tttcgcaact tgattccaat ctgctatcgt aatgtaaggt gtcttctctg
     8281 ccaattctct catataggca gcagccttct caacgatggc ataggtctct tttgtcttat
     8341 ctccctcata aggagtcacc aaaatcatat ggtgtccctt aggaagattt ttcacgatac
     8401 tgtcccagtc atccttgtaa ttctcaggat tatttacccc agtcgcaatg accaccgtct
     8461 taggtaaaaa tttattctgg ctattattta gcatgatttc atttgcggtc ttggttgtta
     8521 cgctgacctg cgcgttaatc tgtgctccag gaagagctgt ctgtagtgct gtatttgccc
     8581 ttaaagccac tgagtcacca attaacatag tgccatcagc aattcccaaa ctgtttgcat
     8641 ctgcccgttc tgccatcacc ttggtctggc caatatttgt tgcagcttgc ttcaagccat
     8701 tgacagtcaa gtctgtctca aacgctccca cttgtggtgc caacaaggtc accatgcaga
     8761 caatgaaggt caagattcct gtacctgctg caagaattgc gtgaatataa ggcaggggac
     8821 gaagagtttg taaaataggt gtgttcttgc ctgcaatcca aggttccaat acataaaatg
     8881 acagactggc aaagccataa gaacaaatca gagtcagtaa tacagcaaga agatttgatg
     8941 tcaactgtga gaaaatgata tagaaaggcc aatggaaaag ataaaccgca tagctagtat
     9001 ccgctaaaaa gctgataatc ttcggctcct gtatatgatg tgtcttttca tgtaagacac
     9061 gcgccgccag aatcatggca agagctgcaa gactggcaag caagaagccg ataagatagg
     9121 caaaaagata agtgaatttg acaaagaaag tcaaaagaac taggaaacca aagcctcctc
     9181 caaaaactac taaagtcttt cgtaaatccc agattttatc caactgcttg acgagggaag
     9241 tcgtctgacg aacgcctaca atagttgcta acatacttcc caaaaagaat ggatagacat
     9301 gagttaaact ggagaaataa acagaggaat aagaggtcac tagaaaacta ccaataaaca
     9361 tggagaagaa actgatcaag aaggcagtag cagataagag aaagatcatc cccttcaact
     9421 gaccatttga tttagcttgt ttggataaga accaaactgc caatccccaa agaatatagt
     9481 agtgaacctc aactgccaaa ctccaattat gaacaaacaa atgaggaatg aactgagatt
     9541 cataactccc acctgttaag agttcataga agttggtcat aaagcctaag acgcccgcaa
     9601 tctggccacc aattccagca acatagtctt ggcgaaccaa gaaagtaaaa ggcatggtca
     9661 ccaagaccat caaaaccaca ggtggcacaa tccgataaaa gcgtcttcta aaaaatccta
     9721 tcaaatcaat ctcattgttc ttagaaaatt cttcaatgag tagagctgta atcaggaaac
     9781 ctgaaaatgt gaaaaagaca tctaccccga aaaatcctcc aggaaagatc gtctgaaaga
     9841 agtgatacaa gagtaccaaa agtaaaccta taatcctaat caaggaaaac catttaatgc
     9901 gcatacgagt ttattcttcc tttcattgta cactttattc tatcaaaaaa agaagaaaaa
     9961 tcctaagaga aaacttagga tttttagctt atatcaattc cattttagaa attacgtcct
    10021 gacttattat agccatattt ttccacaaaa tacttacgga attccaagag attgtcatcc
    10081 atgatggctt gtcgcacttg cttcatcagg ttaagcaaga agtaaagatt gtggtagcta
    10141 gtcaagcgga taccaaaggt ttcatcagcc ttgagcaggt gacgaaggta agcgcgtgta
    10201 tagttattac atgtgtagca atcacactca ggatccagtg gcgtaaagtc ctcagcaaac
    10261 tgggcatttt tcacaaccaa acgtccttga ctggtcatac aagtcccgtt acgagcaatt
    10321 cgagtcggta agacacagtc aaacatatcc accccacgaa tgaccccatc gatcaagcta
    10381 tctggcgctc ccacacccat cagataacga ggtttatttt caggcagcag ttgagttgta
    10441 aagtccaaga ccgcattcat ctcttcatgg gtttctccca ctgccaaacc accgatagag
    10501 tagcctgaga aatccatgct gacaagatcg tgagctgatt ggcggcgaag gtcttcaaat
    10561 cctgcacctt gcacaattcc aaacaaacct tggtcatgtg gacgacggtg agccttcaaa
    10621 ccacactcag cccaacggct ggtacgctcg atcgatttct taacgtagtc ataaggttga
    10681 taaaactgag gacattcatc aaaggacatc atgatgtctg aacccagatt attctgaata
    10741 gagatggctt tttctgggga taggagcatc ttagaaccat ttagatgatt tttaaaggtt
    10801 actccttctt ctgtgatatt acggctatct gctaaagaat aaacctgaaa accaccacta
    10861 tctgtcaaga taggctggtc ccaattcatg aacttgtgga gaccaccagc gcgtgcaatg
    10921 agttcatctc cagggcgaag ccagagatga taggtgtttg ataggataat tcccgaaccc
    10981 atctccttca attcttcagg tgactgagtt ttgacagtgg cttgtgtccc aactggcata
    11041 aacataggtg tcggaaaggt accgtgggga gtgatgattt ctcctagacg agctcctgtg
    11101 tgtttttctt tcttaatcaa acgatatttg attggtgaat ctgacatttt ttacctccga
    11161 agctgggaaa gacagtccca gttcatacag tgtgcccaag gacacactgt atgattttat
    11221 caaaaaaagt aggaactgtc acgaactatg tataagaact atggtataat aagagaatga
    11281 actatccaaa aattgattta aaaacaattc gtcaggagtc aaaacatttt caggctgaca
    11341 ccccccgtct ctttctcctc tatatccttc ccagtatgct ggtcatctta tctggttttc
    11401 ttaatccctt atctcggatc cacgggactg ttttagaaca gccatttttc agcatacttg
    11461 gccaaatact tcaaacctac ctctttcctc tactagtctc ctttatcggg actattcttt
    11521 tgaccagttc cgtctatgca acactgacac tcatgaaaga ttctaaaacg gagccatctg
    11581 taaaaaacag cctagctctc tttgatgagg agcgcttttc tcaaaccttt ttgaccctac
    11641 ttctcaaacg tttctatctc tttttatgga gtatccctaa tttacttggg atttacctcc
    11701 tcttttatag tagcttttta gccaaaaaat tcgtcaccct acatcctgag tttccaaatc
    11761 tagatctctc atcagttgaa accgagcgtt tcctcatggt ctttggactc tactttcttg
    11821 caagtctcat cttgatcatt gtaggaaata tcctctatat tccacagtat tatgcctatt
    11881 cacaggtgga atttcttctc tgttatagtc tagacctagg acaggtccca ccaagacgaa
    11941 tcctaaaaac tagccgttcc tttatgaagg gctacaagtt ccagcacttt gtcctagatt
    12001 tacagcttct tccttggtac ttcctcaatt ggattacctt tggcattgct agtttttcac
    12061 tcctacctta tatccaatgc actaaaatta tgttttaccg agcagtattg gctcgaaaac
    12121 gtccaaaagc ttgaaggttt ttcccctcaa gcttttttct atcaatgagt ttctccgcct
    12181 accaacttga gttcctgatc tccatgttct atcatagcgc cgattgctgc ttctatccct
    12241 cgccgaatat ccactaaact catagttgga gtagtcggtc tgttcaccac ctgttccatc
    12301 atataaggaa tatgcataaa acctgcctta acatatggaa atttcttttc taccaaatag
    12361 agagcctgat acatcaaatg gctgcagaca aaagtccctg ccgtattgga aacagaggcc
    12421 ggtaagccct ctttttttat agcttgaacc atcgctttaa tcggcaaact actaaagtag
    12481 gccgaagcac catctgggcg aatgggacgg tcaatcggtt gattatcttc gttatcagaa
    12541 atgcgtgcat cgtcttgatt aatggccact cgttcaggtg tcacactagt tcttccacca
    12601 gcttgcccaa tacaaaggac aaagtcaggt tgataacgat tcatctcttc ttccaatact
    12661 tgagcagatt tgtgaaaaac tgtcggcacc tctagccaac ggacctcagc accatggatt
    12721 tcagctggta aacctttaat ggcctccaaa gttggattgc ccttttcccc tccaaagggc
    12781 tcaaaacctg tcactaatac tttcatttct aactcctcaa aaccaattca atgagacttt
    12841 tttcttaaaa acaagtataa catatttccc ttattctgga ttaaaaagga ttagcgagat
    12901 taaaatcgac cttcaagtgt atttaaagag taaaatacta atcaaggcca aaatagctga
    12961 tccaccttgc ttcaaaataa tttttttatc cgctgttaaa gagccgtaag tcgcagcacc
    13021 aatcacaaat aagacaaaaa tagtcacaat ttctaaattc tgtgagaaat aaatgccata
    13081 ccagagaaag actcctagca gagccttata aatcccttga tttttgaaca atgaacttac
    13141 tgacggatga gccaattctt ccttttccat attaaataca cgactagtcg catctgatcg
    13201 cgtagcaata ctttccaaat aaaaaatgta aaaatgctcc aaagcaacga tcgttgttaa
    13261 aataattgta ataattgaca tctatttctc cttaaatttc tatattttca atacccaaaa
    13321 ccaatttatt taataaacgg ctgagttctg ttctctcaaa ctctgttaag atactttcca
    13381 tctcttcctt aaccttgata tgttgcagag ggggattcac cactaactgc tccttggcat
    13441 acttcgcagc ctctaccaac acttcccgct gattttcagg attacgatga cgctccacca
    13501 aaccttccgt ttccaaaatc ttgaaatgcc gtgtcaaagc agcctgatca attttcaaac
    13561 gctcctgaac cgccatttgg ttacaaggag aatgctccag caaaaacagt aaaatctgat
    13621 accgtgtcaa actaatccca agcctttttt caaacaattg cgtactcgct tgctcagaca
    13681 agcggagttg atacagtaaa tcttgaatct ctatcatttc ttcctcctat ccattgactt
    13741 atcaatagtt gaccaatcaa ttataatata aagtatagtg aaatgaaata aga
//
LOCUS       DAVBHS010000046        13195 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVBHS010000046 DAVBHS010000000
VERSION     DAVBHS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13195
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.046"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            256..603
                     /gene="ssrA"
                     /locus_tag="VQ651_000964"
     tmRNA           256..603
                     /gene="ssrA"
                     /locus_tag="VQ651_000964"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(786..873)
                     /locus_tag="VQ651_000965"
     tRNA            complement(786..873)
                     /locus_tag="VQ651_000965"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(837..839),aa:Ser,seq:gga)
     gene            1035..1775
                     /locus_tag="VQ651_000966"
     CDS             1035..1775
                     /locus_tag="VQ651_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2753981.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNITVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1772..2683
                     /gene="pfkB"
                     /locus_tag="VQ651_000967"
     CDS             1772..2683
                     /gene="pfkB"
                     /locus_tag="VQ651_000967"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HET2753982.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDVVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            2680..4632
                     /locus_tag="VQ651_000968"
     CDS             2680..4632
                     /locus_tag="VQ651_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2753983.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            4796..7021
                     /locus_tag="VQ651_000969"
     CDS             4796..7021
                     /locus_tag="VQ651_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET2753984.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(7066..7494)
                     /locus_tag="VQ651_000970"
     CDS             complement(7066..7494)
                     /locus_tag="VQ651_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753985.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            7632..8774
                     /locus_tag="VQ651_000971"
     CDS             7632..8774
                     /locus_tag="VQ651_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2753986.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            8783..9997
                     /gene="thiI"
                     /locus_tag="VQ651_000972"
     CDS             8783..9997
                     /gene="thiI"
                     /locus_tag="VQ651_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HET2753987.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVTFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            10132..10956
                     /locus_tag="VQ651_000973"
     CDS             10132..10956
                     /locus_tag="VQ651_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2753988.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYFECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            10978..11717
                     /locus_tag="VQ651_000974"
                     /pseudo
     CDS             10978..11717
                     /locus_tag="VQ651_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            11730..12896
                     /locus_tag="VQ651_000975"
     CDS             11730..12896
                     /locus_tag="VQ651_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET2753989.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
BASE COUNT         3831 a         2524 c         2982 g         3858 t
ORIGIN      
        1 attcgttcta ttcttatttc attttactat attttggtgc aataagtgaa aagtagtccg
       61 aataatataa ggattgattt tatagttttt aaactcaaat gaattgaaat aaagagagta
      121 cgaaaattct catctgaaag tattttagaa taattctctt cgtgaatttc ttcaaaacag
      181 atagcttcat cttaggtatg tgatttcttt ttgcattttt gagttagata aggtataatg
      241 gttttattgt cttttggggt cgttacggat tcgacaggca ttatgaggca tattttgcga
      301 ctcgtgtggc gacgtaaacg ctcagttaaa tataactgca aaaaataaca cttcttacgc
      361 tctagctgcc taaaaaccag caggcgtgac ccgatttgga ttgctcgtgt tcaatgacag
      421 gtcttattat tagcgagata cgattaagcc ttgtctagcg gtttgataag agattgatag
      481 actcgcagtt tctagacttg agttatgtgt cgaggggctg ttaaaataat acataaccta
      541 tggttgtaga caaatatgtt ggcaggtgtt tggacgtggg ttcgactccc accggctcca
      601 ttattccttt gcattctttt gcattccttg gtaaaacgtt gttaaatcaa cgttttttat
      661 ttttatcttt ggtattcctt tgcattcttt tgctaaaaag ggagtcacaa acagacccta
      721 ttttaaaaaa aggatagaaa aaaggataca acatttgtcg catcctaaaa ataatctttt
      781 ttccacggaa gacatgggat tcgaacccac gcacgctatt acacgcctac cgcgtttcca
      841 acacggcctc ttaagcctct tgagtaatct tccaatactt actcaaatag tctaccataa
      901 aggctcttat cttgcaataa aaattctaga aataagaaaa atgatagatt ttgaaagaaa
      961 atgataaaaa atgcttgact tcaaaagaaa gtatgataga atgaatagtg taaacgataa
     1021 caggaggtga ttcggtgtta aaaacagagc ggaagcaact gattttagag gagttaaatc
     1081 aacatcatgt agtttctcta gaaaaattag ttagtttgct agaaacgtca gaatcaacgg
     1141 ttcgaagaga cttggatgag ttggaagcgg aaaacaagct tcgtcgtgtg catggtggag
     1201 cagaactccc ccactcctta caggaagaag aaaccattca agaaaaatct gtcaaaaacc
     1261 ttcaagaaaa gaagttgctg gctcagaaag cagcctctct cattaaagaa aaagatgtca
     1321 tctttatcga tgctggaaca acaactgctt ttttgattca tgaattggtc aataagaata
     1381 ttacagttgt gaccaactcc attcaccatg ccgctcagtt ggttgaaaag cagattccaa
     1441 ctgtcatggt tggaggaaac gtcaagacgg cgacagatgc tagtatcggt ggcgttgctc
     1501 ttaaccagat taaccaattg cactttgacc gtgcctttat cggaatgaat ggtgttgacg
     1561 atggctatta tacgactcct gatatggagg agggagctgt gaaaagagct attttggaga
     1621 atgccaagca gacctacgtc ttggtggatt cgtcaaaaat tggacaaact tgctttgcca
     1681 aggtagcacc actcaaacgc gctatcgtta tcactagtca agggcatgag ctcttgcagg
     1741 ttattaagga gaaaacggag gtaatagaag tatgatttat acagtcacac tcaatccatc
     1801 cattgactat atcgttcgtt tggaccaagt caaagttggt agtgtaaatc gtatggacag
     1861 tgatgataag tttgctggtg ggaaaggaat caatgtcagc cgtgtcttga aacgtttgaa
     1921 tataccaaat acagcgacgg gatttatcgg tggctttact ggtaaattta tcacagatac
     1981 tttagcagag gaagaaatcg agacacgttt tgtccaggtg gcagaagata ctcgtatcaa
     2041 tgttaaaatc aaagcagacc aagaaacaga aatcaacgga acgggtccaa ctgttgaatc
     2101 ggttcagcta gaagaattga aagctatttt atctagtctg acagcagaag atacagttgt
     2161 ctttgcaggt tcaagtgcta aaaatctagg caatgttatc tataaggatt tgatttcctt
     2221 gacgcgccag actggtgcgc aagtggtctg tgactttgaa ggacagacct taattgatag
     2281 tttggactac cagcctcttc ttgtaaaacc aaacaatcat gaacttggag cgatttttgg
     2341 ggttaaactc gaaagtttag atgaaattga gaaatacgct cgtgagttac tggctaaggg
     2401 tgctcaaaat gttattatct ctatggctgg tgatggtgcc cttcttgtca catctgaggg
     2461 agcttacttc gctaaaccaa tcaaaggaac agtcaaaaat tcagttggag ctggtgattc
     2521 tatggttgct ggattcacag gtgaatttgt caaatcaaaa gacgtagtag aagccttcaa
     2581 atggggagtg gcttgcggaa cggcaactac cttctcagat gacttggcaa cggcggaatt
     2641 tattaaagaa acatatggaa aagttgaggt agaaaaacga tgaaaattca agacctattg
     2701 agaaaagatg tcatgttgct agatttgcag gcaactgaaa aaacagctgt catcgacgag
     2761 atgattaaaa atttgacaga ccacggttat gtaacagatt ttgaaacatt taaagaagga
     2821 attttggcgc gtgaagcttt gacttctact ggtttgggtg atggaatcgc aatgcctcac
     2881 agcaaaaacg ctgctgtcaa agaagcgaca gttctatttg ctaagtcaaa taagggtgtt
     2941 gactacgaga gcttggatgg acaagcaact gacctcttct tcatgattgc agctccagaa
     3001 ggtgccaatg atactcactt ggcagccttg gcagaattgt ctcaatactt gatgaaagac
     3061 ggttttgcag acaaacttcg tcaagcaaca tctgcagacc aagttatcga actttttgac
     3121 caagcttcag aaaaaactga ggaacttgtt caagcacctg ctaatgactc tggtgacttt
     3181 atcgtagctg ttacagcttg tacaacaggt attgcccaca cttacatggc ccaagaagcc
     3241 cttcaaaaag tagctgctga aatgggggtt ggtatcaagg tcgaaaccaa cggtgctagc
     3301 ggtgttggaa atcaactaac tgcagaagat atccgtaagg ctaaagctat tatcattgca
     3361 gcagacaagg ccgttgaaat ggatcgattt gatggaaaac cattgatcaa tcgtccagtt
     3421 gctgacggta tccgtaagac agaagagcta attaacttgg ctctttcagg agatactgaa
     3481 gtctaccgtg ccgctaatgg tgccaaagct gcaacagcct ctaacgaaaa acaaagcctt
     3541 ggtggtgcct tgtacaaaca cttgatgagt ggtgtatctc aaatgttacc attcgttatc
     3601 ggtggtggta tcatgattgc ccttgccttc ttgattgacg gtgctttggg tgttccaaat
     3661 gaaaaccttg gcaatcttgg ttcttaccat gagttagctt ctatgttcat gaaaattggt
     3721 ggagctgcct ttggtttgat gcttccagtc tttgcgggtt atgttgccta ctctattgct
     3781 gaaaaaccgg gtttggtagc aggtttcgtg gctggtgcta ttgccaaaga aggttttgcc
     3841 tttggtaaaa ttccttatgc cgcaggtggt gaagcaactt caactcttgc aggtgtctca
     3901 tctggtttcc taggtgccct tgttggtgga tttatcgcag gtgccttggt tcttgccatc
     3961 aagaaatacg ttaaagttcc tcgttcactc gaaggtgcta aatcaatcct tctattgcca
     4021 cttcttggaa caatcttgac aggatttgtt atgctagctg tgaatatccc aatggctgca
     4081 atcaacactg ctatgaatga cttcctaggc ggtcttggag gaggttcagc tgtccttctt
     4141 ggtatcgtcc ttggtggaat gatggctgtt gacatgggtg gaccagttaa taaagcagct
     4201 tatgtctttg gtacaggtac gcttgcagca actgtttctt caggtggttc tgtagccatg
     4261 gcagcagtta tggctggagg aatggtgcca ccacttgcaa tctttgtcgc aactcttctt
     4321 ttcaaagata aatttactaa ggaagaacgt aactctggtt tgacaaacat catcatgggc
     4381 ttgtcattta tcactgaggg agcgattcca tttggtgccg ctgacccagc tcgtgcgatt
     4441 ccaagcttca tccttggttc agcagtagca ggtggactcg ttggtcttac tggtatcaaa
     4501 ctcatggcgc cacacggagg aatcttcgtt atcgccctta cttcaaatgc tctcctttac
     4561 ctcgtttctg tcttggtagg agcaatcgta agtggtgtgg tttatggtta cctacgcaaa
     4621 ccacaagcat aaaaaataga aaaatgaaaa gattggaccg tttggtgcag tctttttctc
     4681 ttcccgaaat gcctgtgaaa tatggtataa tagaagaatg gcaaacaaga atacaagtac
     4741 aacaagacgg agaccgtcta aagcagaact ggaaagaaaa gaagcgattc aacgaatgtt
     4801 gatttcgtta ggaattgcga ttttattgat tttcgcagcc ttcaaattag gggctgcagg
     4861 tataaccctt tataatttaa ttcgcttgct agtgggtagc ctagcttatc tggcgatatt
     4921 cggcctatta atctatctct tctttttcaa gtggatacga aaacaggaag gactcttatc
     4981 tggctttttc accatatttg ctggcttact cttgattttt gaggcctact tggtttggaa
     5041 atatggtttg gacaagtccg ttctaaaagg gaccatggct caggttgtga cagatctgac
     5101 tggttttcga acgactagct ttgctggagg gggcttgatc ggggtcgctc tttatattcc
     5161 aacagccttt ctcttttcaa atatcggaac ttactttatt ggttctatct tgattttagt
     5221 gggttctctc ctagtcagcc cttggtctgt ttacgatatt gctgaatttt tcagtagagg
     5281 ctttgccaaa tggtgggaag ggcacgagcg tcgaaaagag gaacgctttg tcaaacaaga
     5341 agaaaaagct cgccaaaagg ctgagaaaga ggctagatta gaacaagaag agactgaaaa
     5401 agccttactc gatttgcctc ctgttgatat ggaaacgggt gaaattctga cagaggaagc
     5461 tgttcaaaat cttccaccta ttccagaaga aaagtgggtg gaaccagaaa tcatcctgcc
     5521 tcaagctgaa cttaaattcc ctgaacagga agatgactca gatgacgaag atgttcaggt
     5581 cgatttttca gccaaagaag cccttgaata caaacttcca agcttacaac tctttgcacc
     5641 agataaacca aaagatcagt ctaaagagaa gaaaattgtc agagaaaata tcaaaatctt
     5701 agaagcaacc tttgctagct ttggtattaa ggtaacagtt gaacgggccg aaattgggcc
     5761 atcagtgacc aagtatgaag tcaagccggc tgttggtgta agggtcaacc gcatttccaa
     5821 tctatcagat gacctcgctc tagccttggc tgccaaagat gtccggattg aagcaccaat
     5881 ccctgggaaa tccctaatcg gaattgaagt gcccaactcc gatattgcca ctgtatcttt
     5941 ccgagaacta tgggaacaat cgcaaacgaa agcagaaaat ttcttggaaa ttcctttagg
     6001 gaaggctgtt aatggaaccg caagagcttt tgacctttct aaaatgcccc acttgctagt
     6061 tgcaggttca acgggttcag ggaagtcagt agcagttaac ggcattattg ctagcattct
     6121 catgaaggcg agaccagatc aagttaaatt tatgatggtc gatcccaaga tggttgagtt
     6181 atctgtttac aatgatattc cccacctctt gattccagtc gtgaccaatc cacgcaaagc
     6241 cagcaaggct ctgcaaaagg ttgtggatga aatggaaaac cgttatgaac tctttgccaa
     6301 ggtgggagtt cggaatattg caggttttaa tgccaaggta gaagagttca attcccagtc
     6361 tgagtacaag caaattccgc taccattcat tgtcgtgatt gtggatgagt tggctgacct
     6421 catgatggtg gccagcaagg aagtggaaga tgctatcatc cgtcttgggc agaaggcgcg
     6481 tgctgcaggt atccacatga ttcttgcaac tcagcgtcca tctgttgatg tcatctctgg
     6541 tttgattaag gccaatgttc catctcgtgt agcatttgcg gtttcatcag gaacagactc
     6601 ccgtacgatt ttggatgaaa atggagcaga aaaacttctt ggtcgaggag acatgctctt
     6661 taaaccgatt gatgaaaatc atccagttcg tctccaaggc tcctttatct cggatgacga
     6721 tgttgagcgc attgtgaact tcatcaagac tcaggcagat gcagactacg atgagagttt
     6781 tgatccaggt gaggtttctg aaaatgaagg agaattttcg gatggagatg ctggtggtga
     6841 tccgcttttt gaagaagcta agtctttggt tatcgaaaca cagaaagcca gtgcgtctat
     6901 gattcagcgt cgtttatcag ttggatttaa ccgtgcgacc cgtctcatgg aagaactgga
     6961 gatagcaggt gtcatcggtc cagctgaagg taccaaacct cgaaaagtgt tacaacaata
     7021 aaaaaatagc ttctttccaa gtttggaggg aagctatttt agtggctatt gattgctttt
     7081 attttctgaa gttggcgcat tggactgttt ttcgttttca gtagcaggtt tacttgaagc
     7141 aggagtagaa gagtcctgag ttgctgtttt ctgatcttct tttttctctt ccttgacgct
     7201 agattttggt gtttcctctt gctgtgtttt ttcttgacta gtgttagtct ctttagttgg
     7261 actggtgttt tccttagggg attccttttg gatttctttg acaatggttg tcgtctggct
     7321 tgtcgtaggt tcttttttaa tatttttgtt attatccaag gcgttcataa tcgtgatact
     7381 tgcggtaatc aagccaagga tactagcgat tgtagcaacg gttttttgaa agactgtaaa
     7441 gccttttttg atgtgttctg tttttggttt tgaagttcta cgatagttag acatacattc
     7501 tctcctttga ttatgattta ttatactctg tttctttaaa aaaatcttaa aaaaagagaa
     7561 atttcccttt cttgtcgcaa gctccgtttc atgatacaat agaggaagtg attttagaaa
     7621 gcgtgagaaa catgatttac tttgataatt cagcgacgac caagccttat cctgaagccc
     7681 ttgaaaccta tatgcaggtc gcttcaaaaa ttttaggaaa cccatctagt ctccatcgtt
     7741 tgggagacca agcaacacga atcttagatg cttctcgcca acagattgca gatttaatcg
     7801 gtaagaaaag cgatgaaatc ttctttacct cgggtggaac agaaggggat aactggctta
     7861 tcaagggtgt ggcctttgaa aaagctcagt ttggcaagca catcattgtt tcagccattg
     7921 aacatccagc agtcaaagag tcagccctct ggttgaaaag tcaaggattt gaagtggatt
     7981 ttgctccagt tgataagaaa ggcttggtcg atgttgaggc gttagcaggt ttgatacggc
     8041 atgatacaat cctcgtttcc atcatggctg tgaacaatga aatcggctct atccaaccta
     8101 ttgaggctat ttcagaattc ttggcagaca agccgactat ttccttccac gttgatgcgg
     8161 ttcaggcgct tgccaaaatt ccgactgaaa agtatctgac agaacgggtg gattgcgcga
     8221 ctttctctag tcacaagttc cacggggttc gaggtgttgg ctttgtctat atcaaatctg
     8281 gcaagaagat tacacctctt cttacaggtg gtggccagga gcgagattat cgttcgacaa
     8341 ctgaaaatgt ggcagggatt gcagcgacag ccaaggccct ccgtttgtct atggaaaagc
     8401 tagatatctt taggagcaag actgggcaga tgaaggcagt gattcgccaa gctcttctga
     8461 actatccgga tatttttgtc ttttcagatg aggaaaactt tgcacctcat attctgactt
     8521 ttggaatcaa aggtgttcga ggtgaagtca tcgttcacgc ctttgaagac tatgatattt
     8581 tcatctcaac aacctcagct tgttcatcta aggcaggaaa accagccggt accttgattg
     8641 ccatgggagt ggacaaagat aaggccaagt cagctgtgcg tcttagccta gacttggaaa
     8701 atgatatgag tcaggtcgag cagtttttga ccaagttaaa attgatttac aatcaaacta
     8761 gaaaagtaag ataggagcat tcatgcagta ttcagaaatt atgattcgct acggagagtt
     8821 gtcaaccaag ggtaaaaacc gtatgcgttt catcaataaa cttcgtaata atatttcgga
     8881 cgttttgtct atctataccc aagttaaggt aacagcagat cgcgaccgtg cccacgctta
     8941 cctcaatgga gctgattaca cagcagttgc agaatctctc aaacaagttt ttggaattca
     9001 aaacttttct cctgtttata aggttgaaaa atctgtagaa gttttgaagt cttctgtcca
     9061 agagattatg cgggacatct acaaggaagg tatgaccttt aagatttcta gcaagcgtag
     9121 cgaccacaac tttgaacttg atagtcgtga actcaaccaa acacttggag gggctgtatt
     9181 cgaagccatt ccaaatgtgc aagttcaaat gaaaagtcct gacatcaatc ttcaggtgga
     9241 gattcgtgaa gaagcagcct atctttctta tgaaaccatt cgtggggctg gtggtttgcc
     9301 agttggaact tcaggtaaag ggatgctcat gttgtcagga gggattgact cacctgtagc
     9361 aggttatctt gctcttaagc gtggggtgga tatcgaggca gttcactttg ctagtccacc
     9421 atatactagt cctggtgccc tcaagaaagc gcaggacttg acccgtaaat tgaccaagtt
     9481 tggcggaaat atccagttta tagaggtgac tttcacagag attcaagagg aaatcaaagc
     9541 caaagcgcca gaagcttatt tgatgactct aactcgtcgc tttatgatgc ggattactga
     9601 ccgtattcgt gaggtacgaa atggtttggt tatcatcaat ggggaaagtc taggtcaagt
     9661 agccagccaa acccttgaaa gtatgaaggc tatcaatgct gttaccaaca ctcccatcat
     9721 tcgtcctgtg gttaccatgg acaagttgga aatcattgac atcgcccagg aaatcgatac
     9781 ctttgacatt tcaatccaac cgtttgaaga ctgttgtacc atttttgcac cagatcgtcc
     9841 aaaaacaaat cctaaaatta agaatgcgga gcagtacgaa gcgcgtatgg atgttgaagg
     9901 cttggttgag cgagcagtgg ctggaatcat gattactgaa atcacacctc aagccgaaaa
     9961 agatgaagtt gatgacttga ttgacaatct gctctaattc agaaaatcca aaagaatagc
    10021 gaaaatcagt aaaaaaagtt agttttttct ctaaaaacag gtaaaaaact aacttttttt
    10081 atttttatga tataatgata taaaattttg aatatagaga gttttctgac aatgaatcaa
    10141 tcctactttt atctaaaaat gaaagaacac aaactcaagg ttccttatac aggtaaggag
    10201 cgccgtgtac gtattcttct tcctaaagat tatgagaaag atacagaccg ttcctatcct
    10261 gttgtatact ttcatgacgg gcaaaatgtt tttaatagca aagagtcttt cattggacat
    10321 tcatggaaga ttatcccagc tatcaaacga aatccggata tcagtcgcat gattgtcgtt
    10381 gctattgaca atgatggtat ggggcggatg aatgagtatg cggcttggaa gttccaagaa
    10441 tctcctatcc cagggcagca gtttggtggt aagggtgtgg agtatgctga gtttgtcatg
    10501 gaggtggtca agccttttat cgatgagacc tatcgtacaa aagcagactg ccagcatacg
    10561 gctatgattg gttcctcact aggaggcaat attacccagt ttatcggttt ggaataccaa
    10621 gaccaaattg gttgcttggg cgttttttca tctgcaaact ggctccacca agaagccttt
    10681 aaccgctatt tcgagtgcca gaaactatcg cctgaccagc gcatcttcat ctatgtagga
    10741 acagaagaag cagatgatac agacaagacc ttgatggatg gcaatatcaa acaagcctat
    10801 atcgactcgt cgctttgcta ttaccatgat ttgatagcag ggggagtaca tctggataat
    10861 cttgtgctaa aagttcagtc tggtgccatc catagtgaaa tcccttggtc agaaaatcta
    10921 ccagattgtc tgagattttt tgcagaaaaa tggtaagtta agaaaggaaa aaacgaaatg
    10981 catattgaac atcttagcca ctggagtggt catcttaacc gtgaaatgta ccttaaccgt
    11041 tatggacatg gtgggattcc agttgtggtc tttgcttcat caggtggtag tcacaacgaa
    11101 tactatgatt ttggcatgat tgatgcctgt gcttccttta tcgaggaagg ccttgtccag
    11161 ttctttaccc tatctagttt ggatagtgag agctggttgg ctacttggaa aaatgctcat
    11221 gaccaagcgg aaatgcaccg tgcctacgaa cgttatgtga ttgaggaggc cattctttta
    11281 tcaagcacaa gacaggttgg tttgatggca tgatgacgac aggttgctct atgggagcct
    11341 atcatgcact caatttcttc ctccagcatc cagatgtctt taccaaagtg attgctctca
    11401 gtggtgttta cgacgcacgt ttctttgtcg gtgattacta caacgatgat gctatttacc
    11461 aaaactcgcc agtagattat atttggaacc aaaacgacgg ctggtttatt gaccgttacc
    11521 gtcaggcaga gattgtgctg tgtacggggc ttggagcctg ggaacaagat ggtttgccat
    11581 ccttttacaa gctcaaagaa gcctttgaca agaaacaaat tccagcctgg tttgctgaat
    11641 ggggacatga tgtcgcccat gactgggaat ggtggcgtaa acaaatgcct tatttcctcg
    11701 gtaatctcta tttataaaag gagttaccta tgaattacct tgttatttct ccctactatc
    11761 cacaaaactt tcaacagttt accatcgaac tagctaataa aggcatcaca gtcttgggaa
    11821 ttggtcaaga gtcttacgag caattggatg agcccttgcg caatagcttg accgagtatt
    11881 ttcgtgttga taatcttgag aacatagatg aagtcaaacg tgcagttgct tttctctttt
    11941 ataaacatgg tccaattggc cgcatcgagt ctcacaatga atactggctt gagctagacg
    12001 caacactcag agaacaattc aatgtttttg gtgccaaacc agaggatctc aaaaagacga
    12061 aatataagtc tgaaatgaag aaacttttca aaaaagcagg tgttcctgtg gtacctggag
    12121 ctgttatcaa gacggaagca gatgttgatc aagcagtgaa agaaatcggt cttccaatga
    12181 ttgccaaacc tgataatgga gtgggagcag ccgcaacctt taaacttgag acagaagacg
    12241 atatcaatca cttcaagcaa gaatgggacc attcaaccct ttatttcttt gaaaaatttg
    12301 tcacttccag cgaaatctgt acctttgacg ggctcgtgga caaggatgga aagattgtct
    12361 tctcaacaac ctttgactac gcctatacac cgcttgacct catgatttat aagatggaca
    12421 attcttatta tgtgctcaag gatatggatc ctaaactgcg caagtatggg gaagcaattg
    12481 tcaaagaatt tggtatgaaa gaacggtttt tccatattga gttcttccgt gagggggacg
    12541 attatattac catcgagtac aataaccgcc ctgcaggtgg ttttaccatt gatgtttata
    12601 actttgctca ttccttggac ctttatcgtg gctatgcagc tattgtcgca ggagaggagt
    12661 tcccggcgtc agactttgaa actcagtatt gtttggctac ttctcgccgt gcaaatgctc
    12721 actatgttta ttcagaagag gatttgcttg ccaaatatag ccagcagttc aaggttaaaa
    12781 aagtcatgcc agctgccttc gcggaacttc aaggagatta cctgtatatg ctgaccactc
    12841 cgagtcgaca agaaatggag cagatgattg cagatttcgg acaacgtcaa gaataagaac
    12901 tatcggatta aggaaattaa ctcccttaat ccttttgttt tgtctgataa aaaataagag
    12961 catcccaaca aggtagctat cataaaactt gttcgataac tatttgaagc aggattaggt
    13021 ggtcagaaat taaattttaa tatttcaatt gagtcatagt attgtgtttg cgtatcctta
    13081 aatcagctaa aaggatccat gacgacacct atacgatata gttttcaaga taccaaacaa
    13141 gtctattaat attcaatgaa aatcaaagag caaactagga agctagccgc aggtt
//
LOCUS       DAVBHS010000047        13039 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVBHS010000047 DAVBHS010000000
VERSION     DAVBHS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13039
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.047"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            228..1910
                     /locus_tag="VQ651_000976"
     CDS             228..1910
                     /locus_tag="VQ651_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2753990.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELVKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHRKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            1895..2725
                     /locus_tag="VQ651_000977"
     CDS             1895..2725
                     /locus_tag="VQ651_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2753991.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVASLLLSLQNQGRFYNPWK
                     "
     gene            2918..3421
                     /locus_tag="VQ651_000978"
     CDS             2918..3421
                     /locus_tag="VQ651_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET2753992.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      3475..3709
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3874..4437
                     /locus_tag="VQ651_000979"
     CDS             3874..4437
                     /locus_tag="VQ651_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2753993.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTVAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYILLKPTLKHYER"
     gene            4451..5077
                     /locus_tag="VQ651_000980"
     CDS             4451..5077
                     /locus_tag="VQ651_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET2753994.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRSLTRLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLTLAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGALFIEFPFYDKLRFQWRFKGKQK"
     gene            5189..6061
                     /locus_tag="VQ651_000981"
     CDS             5189..6061
                     /locus_tag="VQ651_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000525616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2753995.1"
                     /translation="MGMLLATRLKNKRKELKLSQKELAEGICEQGQISRMEQGKYSPG
                     SELLFQLSKRLKVSMDYFFEDTEVSSLENIDKFKELSKKFLDEREYESLHYIYELEKS
                     KRARLSAKDQIYLDWIDTLVQFCYLNHQTGAVAKLEKILLSLKKSDFYYLQLGNTLAN
                     FYFDMGQEDTYQELYQLLKENLQSLSVHHLEQLELMIKFRYNYCRYLWLNERTSEAIE
                     EITETIAICKRYNSSYRLADLYCLLGSISEGFAERDRILGFYQVSYQLYKHEENQRMM
                     LEIEKEMKLLESED"
     gene            6191..6328
                     /locus_tag="VQ651_000982"
     CDS             6191..6328
                     /locus_tag="VQ651_000982"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753996.1"
                     /translation="MKRSQVVKRCILIALLLLFLLNVLSGSLSVTTRGVDPRPAYGVS
                     L"
     gene            6752..7666
                     /locus_tag="VQ651_000983"
     CDS             6752..7666
                     /locus_tag="VQ651_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET2753997.1"
                     /translation="MKLYQINDGIQIFESSNLEKIVIRNYRNGKKITIDHQPLLKKLL
                     LFLEVPKREEEILLYLPELSNKTLRSVLTNFMSLNIIHCQDTLIIHKKINILIIGLGT
                     TGGYLADGLCRSNINSKLFLLDKDRVDETNVGRQIYLRDDIGQYKVDVFKQKFSDKEI
                     KVFKIFINSKKDLEKICKREGIDLVIQCGDSPSPRELGKIVNTVCDTLQIPYIVNTGY
                     MSNVVPLPEFYYPNNRYNFNYKHQFSDEKLLFSQVLSKADYMVAIQSSFVMLMQIKNF
                     IFNEEPIYYGYRGYYNSVTLAWEVEKID"
     gene            7659..8672
                     /locus_tag="VQ651_000984"
     CDS             7659..8672
                     /locus_tag="VQ651_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2753998.1"
                     /translation="MTKYLELDYSHSYILEGFRKNDAFIENHKECLDMLSDKIWRDNK
                     YMNTEKNISSPTRTILSRYTCNILSSLFIDISKNSIEKLIVTCENSDLNWIYNYYKSV
                     YNGVFCELRQLIQQYCKVKDKYEKWLFIKAIINQGIRQNEKEVVEFFLKQLKDNNQGI
                     FDYINSFSFYLLKFYSKDKSRIFLEEQLDIDDFLGSDKEDYARSLVFKNYASLLPDTS
                     ELKRNIMEKCLSQTPQDTDLWKEWFETYASQKEIRQKATEIFKHGYSDISLLKLVKIE
                     PDDTESLIRMIILCTSDLNSNIARYLISFLSDGRLKEFLELFVENFDFLNIIEGKTSE
                     INF"
     gene            8656..10248
                     /locus_tag="VQ651_000985"
     CDS             8656..10248
                     /locus_tag="VQ651_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2753999.1"
                     /translation="MRLIFRIIRKDIILYSILTFLLSIAMVSEAFLMQFIVDSIKFGE
                     KKYVLISIFIVIFLILQTLLYYYQQFLTAVLSKKSAYEYRKLIFKNIQQTSLRLVTGE
                     KNDSILASLTTQIDQIEYNYFYSVYWGGYLLCQLVVAIVMSLYLNPLMSVLTICLSLP
                     NLFVALLFKNSLEQKQEELINETNSSIAVTQDLIEGITDWKIANREPNIFRVFESKTL
                     KLLEKQVKVEKFQYIVVSLNQLFSNVLYFGSWIVGGFLIIRGQLSLGSLIAFSQLLAR
                     ISYPVYASSDLLSKYISGKKIVEILSKEFIFSDESRNYPDEFNVISFEHYFTNIDEKD
                     MPFDKEFYKNKKYLLKGKSGSGKTTILKSILREYDDYSGIVKINGVDVRDIKESVLFD
                     NIAYVSQKPHLFSASLRDNLTLFSEDFTDNEIVEVLKFVELSKWANKDCLDMMLSNDD
                     IKLSGGEVKRVSLARALLMNKNILLLDEFSAGIDYETLLKIEKKLIDLDKMIIYVTHV
                     DIDRSGKQFDDVIDLDCLFISS"
     gene            10440..11343
                     /locus_tag="VQ651_000986"
                     /pseudo
     CDS             10440..11343
                     /locus_tag="VQ651_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091364.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11361..11498
                     /locus_tag="VQ651_000987"
                     /pseudo
     CDS             11361..11498
                     /locus_tag="VQ651_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
     gene            11526..11867
                     /locus_tag="VQ651_000988"
     CDS             11526..11867
                     /locus_tag="VQ651_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754000.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLKNHI
                     LPLWIGMVSFF"
     gene            11840..12151
                     /locus_tag="VQ651_000989"
                     /pseudo
     CDS             11840..12151
                     /locus_tag="VQ651_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            12279..12866
                     /locus_tag="VQ651_000990"
     CDS             12279..12866
                     /locus_tag="VQ651_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HET2754001.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
BASE COUNT         4065 a         1966 c         2706 g         4302 t
ORIGIN      
        1 aaccatacct acggcaaggc gaaactgacg tggtttgaag agattttcga agagtataaa
       61 atgatgggga aggctgggca actagtcttt ttcgatgttt atagattagt taggcaagta
      121 agctaggata ggaatctttt tggtgctaag attttaagag aagagactgc aagaagggct
      181 tgatttatga taaaatagaa gtctaagaag cgaatgaaga gagtaagatg aaagaagcta
      241 taattgagtg gaaggatttc tctttccggt atgaaacaca acaagaaccg accttgcaag
      301 ggatagactt gaccatttac aagggagaga aagtcttaat tgttggacca tctggatcag
      361 gtaaatctac cttgggtcag tgtttgaatg ggattattcc caatatttac aagggtcaga
      421 catatggaga atttttgata aagggtcaaa cagcctttga tatgagcatc tatgataagt
      481 ctcatctggt tagcacagtt ttgcaggata cagatgggca gtttattggc ttgtctgtgg
      541 cagaagattt ggcgtttgct ctggaaaatg atgtgacagc cctagatgag atgaaaggtc
      601 gtgtttataa atgggctgaa aagctggacc ttcttccttt actggatcag cgtcctcagg
      661 atttgtcagg tggacaaaag cagcgagtca gtctggctgg tgtcttgatt gatgaaagtc
      721 cgattctctt gtttgatgag ccactcgcca atctagatcc caagtcaggt caggatatta
      781 tcgaattgat tgaccagatt cataaggaag aggggacgac gactcttatt atcgagcacc
      841 gtttggagga tgttctgcat cgccctgtgg atcggattgt cttgataaac gatggtcgta
      901 tcctttttaa tgggagccct gaccagttgc ttgcgactga tttattgact caaaatggaa
      961 ttcgagaacc cctttatcta accactctcc gtcaattagg tgtggactta gtcaaggaag
     1021 aacagttagc gaatctggat aacatgtcta tctcaaaagg tcaggttcag ctgcagaatg
     1081 aactggtaaa agaaactcca gaattgcagt cactctttaa attagaggac gtgtctttct
     1141 cttatgatga tagaccgatt ttaaaatccc tacatttaga tattaaaaag ggtgaaaaga
     1201 ttgctattgt cggaaaaaat ggagcaggga aatcaactct agccaaggct ataagtagct
     1261 ttattcagac ggaaggacgc tatctttggg aaaaacagga tataaaaggc gattctgttg
     1321 cagagcgggc ggaacgagta ggctatgtgc tacaaaatcc taatcaaatg atttcaacca
     1381 atatgatttt tgatgaggtg gctctagggc tccgtttgcg aggtgtggat gagaaggaaa
     1441 ttgaaacgag agtctatgaa accttgaaaa tctgtggact ttatgaattc cgtaattggc
     1501 ctatttctgc cctgtcattt ggtcagaaaa aacgtgtcac cattgcttca attttggtct
     1561 taggagctga aattattctc ctagatgaac cgactgcagg tcaagatcag aagaactata
     1621 ctgagattat ggaatttctc gaagagttac atcgaaaagg gcataccatt gtcatgatta
     1681 cccatgatat gcaattgatg ctggattatt cagaccgagt ccttgtcatg gtggatggag
     1741 aattgattgc cgatactgtt ccagccagtc tgttgagcga tcctgagctg ttagtaaaag
     1801 ccaatctaaa agaaacctcc atctttaact tggctaagaa actagatgtg gatccactgg
     1861 atttaacggc attttacaaa gaaaggagag agggatgcaa gctaaattaa tcggttacca
     1921 gcatagagat actgtgattc atcgcttgtc aggagctggg aaacttctct ttttcattct
     1981 ggtatcattg gcggccatga ttagctatga taccagaatg ctcttgctga ttgccatctt
     2041 ttcagtcttt ctcctctatt tatcagaaat tcgctttaaa gatgtttcct ttgtagccgt
     2101 ttttgcgacg gtatttgccg ttttaaacgt tttgatggtc tatctctttt ctcccgagta
     2161 tggggttgga ctttatggag agagaagtgt gatttggcag ggaatcggtg tctacactct
     2221 aaccagccag gagctctttt atctgctaaa tttggccatt aagtatcttt gcaccattcc
     2281 tctggctatt atctttttga tgacaaccca tcctagtcag tttgcttcca gtttaaatca
     2341 aattggtgtg ccctataaga ttgcttattc tgtcagcctg accttgcgct atattccaga
     2401 tttgcaggaa gaattcttta ctatcaagat gtctcaggag gcgcgtggga tggaattatc
     2461 caagaaagct tctcttatgc aacgaatcaa aggcaatctg ctcattatta cgcccttgat
     2521 ttttagctcg ctagaacgca ttgataccat tgcgactgcc atggagcttc gccgctttgg
     2581 gaaagaaaaa aaacgcacat ggtatagtta tcaggccttg aaaaaaggag actatattac
     2641 cttgctcttg gcaaccttgt ttttagtagc tagtttacta cttagcttgc agaatcaggg
     2701 acgattttac aacccttgga aatagcttga aaaaattgaa aaaatcaagt cgtttctatt
     2761 gacaatgatt ctgaaagtgt tatactaaga aagtagtttc gctgatttac ttcaaacctg
     2821 ttgtgaggta agttaacgat gccttaacca cgctgtttgc tgagcttgac tccgggcagt
     2881 gtggctattt ttttgcaatg gtgaaaggaa gcaagtcatg acaaatcaca ttgtattatt
     2941 tgaacctcaa attccacaaa atacgggcaa tatcgcgcgt acttgcgctg cgaccaattc
     3001 tcccctccac atcatcaagc ctatgggctt tcctattgat gaccgcaaga tgaagcgggc
     3061 tggattggat tactgggata agctagagat ttatttttac gagagtttgg aagatttcat
     3121 atctcagatg aagggaaaac tctatctgat ttcgaaattc gcggaaaaag tctattctga
     3181 ggtggattta tcgactgacg aggaccatta ttttctcttt ggacgtgaag acaagggctt
     3241 acctgaagat tttatgcgag aacatcctga gaaggctctc cgtattccca tgaatgatga
     3301 acatgtccgc agtctcaatg tgtctaatac cgtctgcatg attgtctatg aggctcttcg
     3361 ccagcagaac tttgcaggtc ttgaactcgt ccatacctat gaagtggata aattgaaata
     3421 aaaaatgaaa atgaacaaaa tgcttgcgct tgcaagcgtt ttttgttatg ataaaagagt
     3481 cttcagggca gggtgtgatt cccgaccggc ggtgacttta actaggaaat gatcttttcc
     3541 ttttatactt tgttgacaag ctttgcctaa ccagaagtta tgcctacagc ttgtcgccta
     3601 gtctaaaaga aaaatctcta tttccttctc tttaaagaag tccgcgagcg caagctgatg
     3661 tggtgagatt ccacaaccga cagtatagtc tggatgggag aagacgaaag aatagctttg
     3721 tctgttttga tagatttata gccggattgt aaccacttgc tttgtttttc tttaatagag
     3781 taggagggaa cttttgggat ataaaaagtg agaatagata gaggaatcct ttccaacttc
     3841 ttctgatttt atagaaaatt ggaggaacct gttatgacaa acacacgtcg actttcgacc
     3901 attgcaattt tatcagccat ctcatttgtg ctgatgtact ttgactttcc gcttttacca
     3961 gcggcatctt tcctcaagat cgaatttagt atcttgccag tccttgtggg tctggtggtc
     4021 atggatttgc ctgctgctct aggagtgctc ttgcttcgct cactcttgaa attgcttctt
     4081 aacagccaag gagtgaatac ttacattggt ttgccgatga atatcgtagc tttgggagtt
     4141 tttgtcatcg tatttgcttt gatttggaaa aaggaacgga caacccttcg tttcctacta
     4201 ggctctctag ctgggactat tggtttaacc gtggctatgt tagttctcaa ctatgtttat
     4261 gctgttcctt tgtacgctaa gtttgctaac tttgatattg gaaaaatttt gggactttcc
     4321 aactacctaa tgaccatggt attacctttt aacttgattg aaggtgtaat cttttccgtt
     4381 tcattctggt tgttgtacat tctcttgaaa ccaaccttaa aacattatga aagataaaca
     4441 aacattttta atgaagggca gttttgccct tttacttttc gttattcttg gctatatggt
     4501 caaattttac cctgaaatgt tggtcaattt tgaccaatcg attcagactg ccattcgagg
     4561 agacttacca gattatttga ctattctttt tcgatccctc acacgtctga ttgatattcc
     4621 agtgattatc acttgggttg tcattatagc ttttgtcttt tatcgtaagc gatggaagat
     4681 agaaagtttc ttcatgctgg gaaatctgac tttggcaggt cttttaatcg tgacctttaa
     4741 aaatatctac cagcgcccac gaccagctat cttacatctg gtggaggaga agggattttc
     4801 cttcccaagt gggcattctc tggctgtaac gctgatggta gggactttga ttgttattct
     4861 tagtcagcgg attaaagatc aggtctggag aaaaatcgta caaatcgtcc ttggcctcta
     4921 cctagtcagt gtgctggtat caagagtcta tctgggagtt cattacccat cagatgttct
     4981 tgccagtctc tgtgtgggct tgggagccct gtttatcgag tttccctttt atgataagct
     5041 ccgcttccaa tggcgattta aaggcaaaca gaaatgatgg aaaagataaa aaattgtaca
     5101 aatacaaata gaaccatatg aaatatatac ttgaaacatg ttttggagtt tgatatattt
     5161 taataaataa agcaaggagg aagaaagtgt gggtatgtta ttagcgacac gcttaaaaaa
     5221 taaaagaaag gaattaaaat tatctcaaaa agaattagct gaggggattt gtgagcaagg
     5281 gcagattagc cgtatggagc aaggtaaata ctcacctggg tcagaattac tctttcaact
     5341 atctaagcga ttgaaggtga gcatggatta tttctttgaa gatactgagg tttctagttt
     5401 agaaaatatt gataagttta aagaattatc taagaaattt ttggatgaga gggaatatga
     5461 atctctccat tacatctatg aactggaaaa atctaaacgt gctcgtctat ctgctaaaga
     5521 ccagatttat ttggattgga ttgatacatt agttcaattc tgttatttaa atcatcaaac
     5581 aggggcagtt gcgaaactgg aaaaaatact gttatctctg aaaaaatctg atttttacta
     5641 tctacaactg ggaaatacct tggctaattt ttattttgat atggggcagg aagacacata
     5701 tcaggaactc tatcaacttt taaaagagaa cctccagtca ctatctgttc atcatttgga
     5761 acagttagaa ttgatgatta aattccgata taactattgt agatatttgt ggttgaatga
     5821 acgaacatct gaagctatag aagaaattac agaaacgata gctatttgta aaagatataa
     5881 tagtagttat cgattagcag atttgtattg tttattggga agtataagcg agggctttgc
     5941 tgaaagggat aggatactag gtttttatca agtatcttac cagctttaca agcacgaaga
     6001 gaaccagaga atgatgctag aaattgagaa ggaaatgaaa ttattggaaa gtgaagatta
     6061 agcaaaaaga tttgtattct tagataactt taatacatat tataaaaaaa ataaaaaaat
     6121 atcataaaaa ttattgacaa accaaaattg atgtgttaga atatcaccaa tagttaagga
     6181 ggtttagaag atgaaaagat ctcaagttgt caaacgatgt atattaattg ctttattgct
     6241 tctatttcta cttaatgtgt taagtggttc tttatctgta acaactagag gagttgatcc
     6301 aagaccagca tatggagttt cgctttaaag ttgtgcatag ttcttctttt atagacggag
     6361 agtttttctc aatagtaagt gattcaaatt ttgatggatg attttccatt aaattaagtg
     6421 attgcgaaaa ctttttgaaa tacattctct ttggatattg aaagtttgtt ttggattttt
     6481 ttatgaagct tatcttagct gttagattgg ggttttatgg gatttgtcct caccgggaaa
     6541 aaatcttaaa tctttgaaga aagaggtttt tatcttatgg ttagagatac atcagaagaa
     6601 attgaagtta ttgacttgtc ttctatgacc gtttcctact catctgttat gctaggaaac
     6661 tcttggatga cgtggaattg atttattaag atagattatt tggtttttac caaaaatctg
     6721 tctttttaat ttttaaagga ctaatggtga tatgaaacta tatcaaataa atgatgggat
     6781 acaaattttt gaaagtagta atcttgaaaa aatagtaatt agaaattata ggaatggtaa
     6841 gaaaataact atcgatcacc aaccattatt aaaaaaatta ctactattcc ttgaagttcc
     6901 taaacgtgaa gaagaaattc tattatatct tccagaatta tcaaataaaa cattacgaag
     6961 tgttttgacg aattttatga gtttaaatat aattcattgt caagatacat taataattca
     7021 caaaaaaata aacatattga taataggttt agggactacg ggtggatatc tggcagatgg
     7081 gttatgtcgt agtaatatta attcgaagct atttctttta gataaggatc gtgtagatga
     7141 gactaatgta ggtagacaga tttatttaag agatgatatt ggtcaatata aagttgatgt
     7201 atttaaacaa aagtttagtg ataaagaaat aaaggtattc aaaattttta ttaatagtaa
     7261 gaaagatttg gaaaagattt gcaaaaggga gggtatcgat ttggttatac aatgtggtga
     7321 ctccccttca cctcgagaat taggaaaaat tgtgaataca gtatgtgata ctctccagat
     7381 accttatata gttaacactg gctacatgag taatgtagtt cctttgccag aattttatta
     7441 tccaaataat agatacaatt ttaattataa gcatcaattt tcagatgaaa agttactgtt
     7501 ctcacaagtt ttgtctaaag ctgattatat ggtggcaatt caatcatcat ttgtaatgtt
     7561 aatgcaaata aaaaatttta tatttaatga agaacctatt tattatggtt ataggggata
     7621 ctataattct gtaacattag catgggaggt ggaaaaaatt gactaaatat ttagaattgg
     7681 attattctca ttcatatatt ttggaaggtt ttagaaaaaa tgatgcgttt attgaaaatc
     7741 acaaagaatg ccttgatatg ttatcagata agatttggcg agataataaa tatatgaata
     7801 cagaaaaaaa catatcatca ccgacacgaa caatcttatc acgctatact tgtaatattt
     7861 tatcgtcttt gtttattgat attagcaaaa atagcataga aaaattgata gtgacatgtg
     7921 aaaatagcga tcttaattgg atttataatt attataaatc ggtttacaat ggagtatttt
     7981 gtgaattgcg acaacttata caacagtact gtaaggtgaa ggataagtat gaaaagtggc
     8041 tttttataaa agctattata aatcaaggta ttcgtcaaaa tgagaaagaa gttgttgaat
     8101 tctttttaaa acaattgaag gataataatc aaggtatttt tgattacatc aatagtttta
     8161 gtttttatct attaaagttt tattcaaaag acaaatctcg cattttttta gaagagcagc
     8221 tagatataga tgattttttg ggatcagata aagaagatta tgctaggtct ttagttttta
     8281 agaattatgc ttctctattg cctgatactt ctgaattaaa aagaaatatt atggaaaaat
     8341 gtttaagtca aacacctcaa gatacagatt tatggaaaga atggtttgaa acttatgcca
     8401 gtcagaaaga gattagacaa aaagcaacgg aaatatttaa gcatggttat tcagatattt
     8461 cgttactaaa attagttaaa attgagcctg atgacactga gagtttaatc agaatgatta
     8521 tcttatgtac cagtgaccta aatagtaata ttgcaaggta tttgatttca tttttgagcg
     8581 atgggcgatt aaaagaattt ttggagttat ttgttgaaaa ttttgacttt ttgaatatta
     8641 ttgagggaaa aactagtgag attaattttt agaataatta gaaaagatat tatattatat
     8701 tctatattaa catttttgct atcaatagcg atggtaagcg aagcattttt gatgcaattt
     8761 attgttgatt caattaaatt tggtgaaaag aagtatgttt tgatttctat ttttattgta
     8821 atttttttga ttcttcagac tttattgtat tactatcaac aatttcttac agctgtatta
     8881 agtaaaaaat ctgcatatga gtaccgtaaa ttaattttta aaaatattca gcagacttct
     8941 cttaggcttg ttacaggtga aaaaaatgat agtattctag cttctttgac aacgcaaatc
     9001 gatcaaatag aatataatta tttttattct gtttattggg gtggatattt gctttgccaa
     9061 ttagtggtag ctatagttat gtcgctttat ctaaatccat tgatgtctgt attaaccatt
     9121 tgcttaagtc tccctaattt atttgtagca cttcttttta aaaactcctt ggaacaaaag
     9181 caagaagagc taattaatga gacaaattcc tcaatagcag ttactcaaga tttaattgag
     9241 ggaattactg attggaaaat tgcaaataga gaacctaata tatttagagt gtttgaaagc
     9301 aaaacattga agcttttaga gaagcaagtg aaggtagaaa aatttcaata cattgttgtt
     9361 agtttaaatc aattgttttc aaatgtttta tattttggta gttggatagt aggtggattc
     9421 ttgattatac gggggcaact ttcattaggg agtttgatag cgttctctca attattagca
     9481 agaatttctt accctgttta cgcttcttcg gatttattgt caaaatatat tagcggtaaa
     9541 aaaattgtag aaattctttc taaagaattt atattttcag acgaaagcag aaattatcca
     9601 gatgagttta atgttatttc atttgaacat tattttacaa acattgacga aaaggatatg
     9661 ccttttgata aagaatttta caaaaataaa aaatatttgt tgaaagggaa aagtggtagt
     9721 gggaaaacaa caattttaaa atcgatttta cgagagtatg atgattatag tggaattgta
     9781 aaaattaatg gggtagatgt tagggatata aaagagagtg ttctttttga caatatagct
     9841 tatgtttccc aaaaacctca cttgttcagc gcaagtctta gagacaacct gacattattt
     9901 tctgaagact tcactgataa tgagatagtt gaggttttaa aatttgttga attatctaaa
     9961 tgggctaata aagattgctt ggatatgatg ctttcaaatg atgatataaa attatcagga
    10021 ggggaggtca aaagggtttc tttagctcga gccttattga tgaataagaa tattctgtta
    10081 ttggatgagt tctctgctgg gattgattat gaaacattgt taaaaataga gaagaaactc
    10141 attgatttgg ataagatgat tatttacgtg acgcatgtgg atatagatag atccggcaaa
    10201 cagtttgatg atgtcattga tttggattgt ctttttatat cctcgtgaca tgagagttga
    10261 gtcagttgaa ttattttgtc actctgttta gttcttgttg gtagtgtcag gtaatctttg
    10321 ttaatttttc aggaagtcta ttgaaatatc ctcttggtta tatattacga ggagtaatta
    10381 atggatattc tcgagaattc aatacaacaa tttgattcta aagttaagta tgtttcttta
    10441 tttgcggaat caatctttta tcagatacca tgagctattt tgcaggtgct atgatttctc
    10501 ctttgtatgt gaggattatc ggagatgata tgacagatgc tatgggggtt agacaggcaa
    10561 ctgctggtgt agttaatatt ctcagcaacc ttataggggg agttttgatt gggggtcatc
    10621 agtcttcaga tgtttgtagg tttgaatgct ttgacctttc tttttgcact gttgggtgtg
    10681 gtgtttattc gtacttcttt gagagatatt gaggaacaga tggaggttag taaaagatta
    10741 gccgcaaaat cttttggaga gcatttcctc cattcaatga agatattgtc aagttttcca
    10801 gccatcatca aattaatatt cattgcagct atgaaccaag tgattttaag cattattatc
    10861 cctttaacga ctttgatgtt aatccatcat ccatttatct tctttgacac tgggaaatcc
    10921 attgcagcat tgtcagtggt tattttttca ggaacgattg tgggaagttt tttgagtgga
    10981 ggtatcctaa aaaatattcc cataaaaatg attgtctact ttgaagaaat tatgcatctc
    11041 tgtattctct tttcttggtt gaccaatcat ttcctccttt tattggtagt tgtcttctta
    11101 tgcgcagttg cagtaggaat gatggggcct cggattttca accttgtatt ctcgatggtt
    11161 ccagaagaag tcatgggaac catccagtca gtgcttggag tattcaatgt tgttgtacca
    11221 ggaattctta gcatgctcat cgttgcctta gcgagtgcta caaacctaaa aatcacagtg
    11281 cttccacttt ttatttttgt attgactgcc ttagcaattg tgaaagcaat gaaaatagag
    11341 taatagtagt atgaactact gtctcttttt gggtgggagt tcattaccca tcagacgtcc
    11401 ttgccagtct ctgtgtgggc ttgggagttt tgtttatcga gtttcccttc tatgacaagc
    11461 tccgcttcca atggcgattt aaaggcaaac agaagtgagt atcgaattcc cttgaggaga
    11521 aagaaatgaa agtcaatata gcagatcttc atccgactca actatattta tcagaaaaga
    11581 agttgcaaga tattcagatg ctttaccagt cggcagaaac aatccaagtc gatccaatca
    11641 gtattcttgc ctttggagat tgtttgctga ttacagatgg gcatcacagg gcttatcagg
    11701 ctttattggc aggtcgggat acgatttctg ctgagtggga tagagatggt ggtgatgaac
    11761 tatatcatct ctatgcgcaa gcttgcgagg aaagaaagat ttactctgtt tttgatttaa
    11821 aaaatcatat cttacctctt tggatcggca tggtgagctt tttttgattg aagtcaagga
    11881 gaagggagaa tggtttccaa ttggggatat tacactatct caggataatc tccccattgt
    11941 gattgggaat tccgcttacc aacatcgagg acttggaaaa aagattctaa gtgctttgat
    12001 tgaattggct cgagtaaaag gatggaaaga attgagagtc aaggaaatct acacctacaa
    12061 tcatgcttct aggaggtgtt tcaagtcgct tggatttgtg gaaaatggag caacagaaaa
    12121 aggaaggagt tttatattgg aattagtcta atcctgccac tttctccccc ttaacctttg
    12181 actattccgc aaaattatcg taaaataaag agtaaatgat aaaatgaggt cagagtctgt
    12241 tcgctctgac gatagtagta taaatgagga gaaacgcttt ggaattagaa gtatttgctg
    12301 ggcaagaaaa aagtgaacta tctatgattg aggtagcgcg tgctatatta gaacttcgtg
    12361 gtcgcgatca tgagatgcat tttagcgatc ttgtaaacga aattcaaaac taccttggaa
    12421 catcaaacag cgatatccgc gaagctttgc ctctgttcta cacagagttg aactttgacg
    12481 gtagcttcat ctcacttggg gacaacaaat ggggtcttcg ttcatggtat ggtgtggacg
    12541 aaatcgacga agaaatcatc gctcttgaag aaaatgacga cgatgaagta gcaccaaaag
    12601 ctaagaaaaa acgtgtcaat gcctttatgg atggtgattc agatgccatt gactacaatg
    12661 cagatgatcc agaagacgaa gatgcatacg aagcagatcc agctctttca tacgatgatg
    12721 aaaatccaga tgatgaaaaa aatgaagtgg aagcttatga tgcagaaatc aacgaaatcg
    12781 ctccagatga cttgggagaa gatgtggatc tcaacgaaga cgacgacgag ttttcagatg
    12841 acgacgctga aaccagcgag gaataagagt tagctattga caattatcct atttttaggt
    12901 atcatattgt ttgggcacct cttttagagg tcggggctcc ctagttctta gggagctatt
    12961 tttgtttttt caagaagtta tcttcttgta ttttatactc aatgaaaatc aaagagcaag
    13021 ctaggaaact ggccgtagg
//
LOCUS       DAVBHS010000048        12487 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVBHS010000048 DAVBHS010000000
VERSION     DAVBHS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12487
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.048"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(251..433)
                     /locus_tag="VQ651_000991"
     CDS             complement(251..433)
                     /locus_tag="VQ651_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754002.1"
                     /translation="MAVIFKNVLIEEKTEEEKQHILSILNGEYDLSDDVSCRLRRLNE
                     ESKRLEVFVKETNSLV"
     gene            complement(649..1881)
                     /locus_tag="VQ651_000992"
     CDS             complement(649..1881)
                     /locus_tag="VQ651_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET2754003.1"
                     /translation="MKIAVAGTGYVGLSIAILLAQHHQVVAVDIIPEKVELINKKKSP
                     IQDDYIEKYLAEKELNLVATLNAEEAYENADFIVIAAPTNYDPKKNFFDTSSVEAVIE
                     AVMKYNPDAIIIIKSTVPVGYTKSVRRKFLTDNIMFSPEFLRESKALYDNLYPSRIII
                     GTDKDDKDLVKSAEIFVKMLQEGAVKENIETLYMDFTEAEAVKLFANTYLALRVSYFN
                     ELDTYSEMKGLDTQAIIDGVCLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYSDVPQ
                     EMITAIVQSNRTRKDYIADRILDLAGSYVGNDFYKPDKEKEIVVGIYRLTMKSNSDNF
                     RQSSIQGIMKRLKAKGVTIIIYEPTLESGTTFYGSQVVNDFEIFKKSSQVIVANRFDE
                     TLEDVRGKVYTRDIFKRD"
     gene            complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQ651_000993"
     CDS             complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQ651_000993"
                     /EC_number="5.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate 4-epimerase Gla"
                     /protein_id="HET2754004.1"
                     /translation="MRGKTILITGVAGFIGSNLVRKLLELEGTMTIIGLDNLNDYYDI
                     ALKDYRLLQLNKLIQDYPEKNWIFIKGDIADKMLINKIFKSYHPQIVVNLAAQAGVRY
                     SITNPDVYIESNIVGFHNVLEACRHSYEIYDGGVEHLVYASSSSVYGGNKKIPFATED
                     KVDNPISLYAATKKTNELQAYAYSKIYNIPSTGLRFFTVYGPAGRPDMAYFGFTDKLR
                     SGSDIEIYNHGNCKRDFTYIDDIVEGIVRVMQSAPKKLVGSDNLPLAPYKVYNIGNSK
                     PENLLDFVDVLQQELIKAGVLPENYDFDSHKKLVPMQPGDVPVTYADTSDLERDFGFK
                     PKTSLREGLRKFAEWYKDYYNV"
     gene            complement(3048..4217)
                     /locus_tag="VQ651_000994"
     CDS             complement(3048..4217)
                     /locus_tag="VQ651_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET2754005.1"
                     /translation="MRLSKKYWFALTSKFLLILLTLLSSIIINRGLGVEKKGDYAYVM
                     NLVEIFYILGGVGLGQAYSTFKRNHSNEYQNKFVFLTFIQGLLFGIIGLIIVLADVPI
                     DYGGVIIVLSSLSIMRTNLTMIAVIENSIKRNKIVLGVSVVYSVMLVLLLLFHLVSIE
                     MVFLVYAINEFLRIILFLYYYNMFPVFEHISIKEFFTIYKFGFLSMILTLLITFNYSI
                     DIIMLKQMSSSYNVGIYSVAVTFSNMFLMVPDSFKEILFGDSAKRTFEKNKAINAIKV
                     SFLFMILLIIGFLSFGEFAITLLYGSSYIKAFPITLVIFLGSLSMIFFKILQPIYISQ
                     GKQSVAIKILSVSALINISLNWYLIPNYQITGAAIASAISYTVCGLFFIIDYLRN"
     gene            complement(4403..5707)
                     /locus_tag="VQ651_000995"
     CDS             complement(4403..5707)
                     /locus_tag="VQ651_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HET2754006.1"
                     /translation="MKFSLQSNDIKNKIILLVGVVFSGTLILCSENNDVTIPLFSMIF
                     LSFMAMIIVKFNIFHPYVWYSSFFTIYSISYPLLYRMEYINYGYTKDLIFMQWLALAT
                     LLLTLPSKAIVLSERKINLNNNNYLLFLNNMNTFITTVAIIYLLGSGFRNKGEIYSGA
                     NIVIMLVFSLVYFMILIHTYQLFIQLSTKKWRLLNCNTNTAIVICLFSIVTGERDYMF
                     TLFILDVIILFYFHKIKRFYLVMLVPLAALLIPLSAVFKYTLLSGQVSSINTNNIWFD
                     LLDGEFVSASRNLQILILHNMGNYFEGRSFFNDIVRIFYNTGYSNQTWFMDTFFPNVH
                     STKYGFTLVGEGYVNGGYFGIVMIFMLTGFLMRFLYINAQRNIYGMLIYLYMIPIFIY
                     STRADFANILSPLLKYAILGTLVIVFINRMVLISPLDEMGGE"
     gene            complement(5824..6978)
                     /locus_tag="VQ651_000996"
     CDS             complement(5824..6978)
                     /locus_tag="VQ651_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2754007.1"
                     /translation="MKVFIATEFRCTIYKNEIYLKPKAYFIYERYAKAFGEIVLCSRY
                     EKVESYPEGYHKANFINKFIPIEGLHQVLFGQNKNKIIEGMIDSDLIVVRIPSIIGSK
                     TADYALKIGKPYLTEIMGDAWDSYWYHSLKGKLLAPYIYAKTKSIVKNANYCIYVTEK
                     YLQDRYPNIKSNIVASNVNITSVENRSLKSRLYKLKKFNPQKISIMTTASVNVRAKGH
                     RFVLEAMKRLEIQGILLDYYLAGDGDQSFLKKKAEELGVANRIHFLGELTTSQIYEYL
                     DKVDLYIQPSLQEGLPRAVIEAMSRACPCIGSNKAGIPELLGPHCLFTPSSSQAIAGS
                     IIDFISMDKEKMVIKQFEFSKKFLSSVLNKRRSDYFAQIRRELNNSDGLD"
     gene            complement(6975..7526)
                     /locus_tag="VQ651_000997"
     CDS             complement(6975..7526)
                     /locus_tag="VQ651_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET2754008.1"
                     /translation="MNRIIKNIVGLLRANICLILLKVCRNKDLTFHYFLKFGKNVTVE
                     QESNAKLILGKKIRVNAGGVLKVRKGAKLKISDDVFLSNNCMIACRKYIDIKSGVKCG
                     PGVLIYDHDYDVSVPGGLKAKKFKTAPVMIGENVWIGANSIVLKGVSIGENSVVAAGS
                     VVTKDIPADTIFIQKRLSREMKL"
     gene            complement(7519..8655)
                     /locus_tag="VQ651_000998"
     CDS             complement(7519..8655)
                     /locus_tag="VQ651_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2754009.1"
                     /translation="MNLQKKKIMLITNHDDDIYCFRKEVIESLVEYGYEVLVSCPYGD
                     KIELMKSIQFEYEDITIDRRGINPLKDIRLFLSYIRLLRKYRPDVVLTYTVKPNVYAS
                     LAATILGIKYINNVTGLGSVLTMGSCVRSLVLLLFRISFRKSNCVFFQNEYNMKMLKT
                     LGLILGDSKLIPGSGVNIEKYPVQQYPNGGNGIQGETIVFNFIGRILKEKGIDTYLAA
                     AQIIKSRYPKTEFNIIGFIEPTESNYELKICDLEKKGIVYYLGQQKDVIPHITRSHAI
                     IHPSVYGEGMSNVLLENASSGRVLITTDNPGCKEIVKDRETGYIFQGGNVEELVSILE
                     VFLGLENEKRKEMGLQGRMYIEQYFSRQIIIDAYLDKIKGALDE"
     gene            complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQ651_000999"
     CDS             complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQ651_000999"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HET2754010.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNTLCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIAMSFARAGYKTLLFDGDIRNSVMSGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTPPIGIVI
                     DAAIITQKCDASILITATGEANKRDVQKAKQQLEQTGELFLGVVLNKLDISVDKYGVY
                     GRK"
     gene            complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQ651_001000"
     CDS             complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQ651_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HET2754011.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYLVNRNQGEKPGLTNQDLQAGAYLVKDYREIVLSQDVLEEVISDLKLD
                     LMPKGLANKIKVTVPVDTRIVSVSVSDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNTLIGFLAGVIGTSVIVLLLELLDTRVKRPEDIENALQMT
                     LLGVVPNLGKLK"
     gene            complement(10172..10903)
                     /locus_tag="VQ651_001001"
     CDS             complement(10172..10903)
                     /locus_tag="VQ651_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase CpsB"
                     /protein_id="HET2754012.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKELFVDNPRKIIMDQLI"
     gene            complement(10905..12350)
                     /locus_tag="VQ651_001002"
     CDS             complement(10905..12350)
                     /locus_tag="VQ651_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET2754013.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLICFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLLFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSKIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADS
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLTDGDRDRGRNQQKVIVAIIQKLTST
                     EVLKNYSSILQGLQDSLQTNIPIETMIDLVNTQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLTVVKAAIQDVMEGR"
BASE COUNT         4203 a         2421 c         1643 g         4220 t
ORIGIN      
        1 aattcactta cttcatatta catttagcat gaaatgaaat aagcgaattc tactttgttt
       61 gcatacaatt aaaaatcaat tcctaaggat gttttaaata actgtgtaaa aggcataatt
      121 tcaccatttc ttagtttttc aatgctaaat tacatagtaa ctggagagct attagaaatc
      181 tctaacttcc tcatagttaa aaaatgacaa tgagatactt attgatttca attcattaac
      241 agaatacgaa ctagacaagt gagttcgtct ccttaacaaa cacttccaag cgtttacttt
      301 cctcattcag tcgtcgtaat ctacaactta catcatcaga taagtcatac tcaccattta
      361 gaatagatag aatatgttgt ttttcttctt cagtcttctc ttcaataagc acatttttaa
      421 aaattactgc cattatcagc acctcacttc gaaggaatat atgcaactat atcctattaa
      481 gtcaatctct actgacaaaa gaagtatatt tacttcacta agtgcgagcg agaatataga
      541 aatatcttta ttgttttaga tgagttgtcg ctatcctcca gctagttttt aactattcta
      601 tcattcaaat gatatagatt attagggact agaaatatac aaattgattt aatctctctt
      661 aaaaatatcc ctagtataaa ctttacccct cacatcttct aaagtctcat caaaacgatt
      721 tgcaacaatc acttgactag atttcttaaa tatttcaaaa tcattaacta cttgactacc
      781 atagaaagta gtaccacttt caagagttgg ttcgtaaata ataatagtaa caccttttgc
      841 tttcaaacgc ttcataatac cttgaataga agattgtcta aaattatctg aattactttt
      901 cattgttaaa cgataaattc ctacaacaat ctctttttct ttatctggtt tatagaaatc
      961 attgcctaca taagatccag caagatctaa aatacgatct gcaatataat cttttcttgt
     1021 ccgattactt tgtactatag ctgttatcat ttcttgtgga acgtcagaat aatttgcaag
     1081 aagttgcttg gtatctttag gaagacagta acccccataa ccaaaagaag gattattgta
     1141 gtgagaacct attctcggat ctaaacaaac tccatcaata atcgcttgag tatctagtcc
     1201 tttcatctca gaataagtat ctaactcgtt aaaataggaa acccggaggg caagataggt
     1261 attagcaaaa agttttactg cttcagcttc tgtaaaatcc atatacaatg tttcaatatt
     1321 ttctttcact gcaccttcct gaagcatttt tacaaaaatt tcagcagact ttaccaaatc
     1381 tttatcatct ttatctgtac ctataataat tcgactcgga tataggttat catataacgc
     1441 ttttgactcc cgtaaaaact caggactaaa cataatattg tctgtaagaa atttcctccg
     1501 tactgacttt gtatatccaa caggaacagt acttttaatt attataattg catcaggatt
     1561 atatttcata actgcttcaa tgacagcctc gactgaagat gtatcgaaaa aatttttttt
     1621 cggatcataa ttagttggag ccgcaataac tataaaatcc gcattctcat atgcttcctc
     1681 tgcattcaat gtagctacga gattcaattc tttttccgcg agatattttt ctatataatc
     1741 gtcttggata ggggacttct ttttattgat taactcaact ttctcaggaa tgatatctac
     1801 ggcaacaact tgatgatgtt gagccagtaa gattgcaata gataatccaa catatcctgt
     1861 acctgctact gctattttca taattttttc ctttctcaaa acactctcgt tatacattat
     1921 agtagtcttt ataccactct gcaaactttc tcaaaccttc tcgtaaagaa gtttttggtt
     1981 taaaaccaaa atctctctct aaatcacttg tatctgcata agtaacagga acatcgcctg
     2041 gttgcatagg aactaatttt ttatgactat caaaatcata attttccggc aaaactcctg
     2101 ccttgattag ctcctgttga agaacatcca caaaatctaa caaattttcg ggtttactat
     2161 tcccaatatt gtacacttta tatggggcta acggtaaatt atcagatccc actagtttct
     2221 tcggagctga ttgcatgacc cttactatcc cctcaacgat atcatctata taagtaaaat
     2281 ctcgtttaca attaccatga ttataaatct caatatcgga accacttcgc aatttatctg
     2341 taaatccaaa ataggccata tctgggcgcc cagcaggacc ataaactgta aaaaagcgta
     2401 agcctgtcga aggaatattg taaatctttg aataagcata cgcttgaagt tcatttgttt
     2461 ttttagtcgc agcatatagt gaaataggat tgtctacctt atcttctgtt gcaaacggga
     2521 tttttttatt tccaccatat accgatgagg acgatgcata aactaagtgt tccacaccac
     2581 catcatatat ctcatacgaa tgacgacaag cttccaacac attatgaaag ccaacgatat
     2641 tactttctat atatacatct ggatttgtaa tggagtagcg tactcctgcc tgggcagcta
     2701 gattaacaac aatctgagga tgataactct taaaaatctt attaataagc attttatctg
     2761 caatatcacc cttgataaat atccagtttt tttctggata atcctgaatt agcttattaa
     2821 gttgaagcaa tcgataatct ttaagtgcaa tatcatagta atcattcaaa ttgtccaaac
     2881 caatgattgt catcgttcct tctaattcca ataatttcct aaccagattt gatccaataa
     2941 aaccagctac tcctgtaatt agaattgttt ttcctctcat atcataatac ctcttgttat
     3001 ttttatattt tcgtccacta atacacattc tttaacaact attataatca atttctaaga
     3061 taatctataa taaaaaacaa accacataca gtataagaga ttgctgatgc tatcgcagct
     3121 cctgtaattt ggtagttagg aattaagtac cagtttaaac ttatatttat caaagccgac
     3181 acagataaaa tttttatagc tacactctgt ttaccctgag atatataaat tggttgtaaa
     3241 attttaaaga aaatcatcga taagcttcct aaaaaaatga ctaatgttat aggaaatgcc
     3301 tttatataac tggaaccata aaggagcgta atcgcaaatt ctccaaaaga aagaaatcca
     3361 ataattaata aaatcataaa tagaaatgat acttttattg catttattgc tttatttttc
     3421 tcaaaagttc ttttagccga atcaccaaat aaaatctctt taaatgagtc tggaaccatc
     3481 aaaaacatat tagaaaacgt aactgcgaca ctgtatatac ctacattata actactagac
     3541 atttgcttca acattatgat atcaatacta taattaaatg taattaatag agtaagaatc
     3601 atagaaagaa atccaaactt atatatagtg aaaaattctt taatactaat atgttcaaaa
     3661 actgggaaca tattgtaata gtataaaaat aaaatgatac gtaaaaattc attaatagca
     3721 tataccaaaa ataccatctc tatagaaact aaatgaaata gtaacaacaa tactagcata
     3781 acggaatata ctacgcttac tcctaataca atcttattcc tctttatact attttctatt
     3841 actgcaatca tagtaagatt tgttctcata atagatagac tggacaaaac tattattacc
     3901 ccaccataat ctatcggaac atcagctaaa actattatta aacctattat tccaaacaat
     3961 aatccttgaa taaaagttaa aaaaacaaat ttattctgat actcattaga atgattcctt
     4021 ttaaaggtcg agtaagcctg acctaaacca actccaccta aaatataaaa aatttctact
     4081 aagttcatta cataggcata atctcctttt ttttcaacac ctaatcccct attaattata
     4141 attgaggata aaagtgttaa taagattaac aaaaatttac tcgttaaagc aaaccaatat
     4201 ttcttagata gtctcactta ttttctccgt atgttaaatt ttataacagt aaatttaaaa
     4261 ttatagtata ttgaaataga atatgaacaa aacgattagg gaatttaaag taatttataa
     4321 aaattcttta gaagctgcat tgtactactc caacttcaaa ccactatagt atcaaaaagt
     4381 ataccccacc tttttaaata aactactcac cacccatttc atccaatggt gatatcaaca
     4441 ccattctatt gataaaaaca ataaccaatg ttcccaatat agcatattta agtaatggag
     4501 ataatatgtt agcaaaatca gcacgagtag aataaataaa aataggtatc atatacagat
     4561 atatcagcat cccgtatata tttctttgag cgtttatata aagaaatctc atcaaaaagc
     4621 ctgttagcat aaaaatcatt acaattccaa agtatcctcc attcacatat ccctctccca
     4681 caagagtaaa accatatttg gttgaatgta cgtttggaaa aaaggtgtcc ataaaccaag
     4741 tttgattaga ataccccgta ttataaaaaa ttcttactat gtcattaaaa aaagaacgac
     4801 cttcgaaata attgcccata ttatgtaata tcagaatctg taaattacga ctagctgata
     4861 caaactcacc atctagcaaa tcaaaccaaa tattatttgt atttatagat gaaacttgcc
     4921 cacttaataa agtatattta aaaactgcag ataacggaat cagtagtgcc gcaagcggaa
     4981 ctaacattac aagataaaaa cgttttattt tgtgaaagta aaataatata atcacatcaa
     5041 ggatgaataa agtaaacata taatctcttt cccctgtaac gatagagaag agacagataa
     5101 ctatcgctgt gttagtatta caatttagta gtctccattt tttagtactc aactgtataa
     5161 atagctgata tgtatgaatg agaatcataa aataaaccaa agaaaatact aacattatca
     5221 caatattggc acctgaatat atttcccctt tatttctaaa accacttcct aatagatata
     5281 taattgctac tgttgtgata aacgtattca tattattgag aaataataaa tagttattgt
     5341 tatttaggtt tattttcctt tcgcttaaca caatggcttt agaaggtaga gttaacaaca
     5401 aagttgccaa agccagccat tgcatgaaaa tcaaatcttt tgtataacca tagtttatat
     5461 actccattct atagagtagc gggtaagaaa tagaataaat agtaaagaag ctactatacc
     5521 acacataggg atgaaaaata ttaaatttca caattatcat tgccataaaa cttaaaaaaa
     5581 tcattgaaaa taatggaatt gtaacatcat tattttcact gcataaaatt aaagtccctg
     5641 aaaaaactac acctactaga agtattattt tattttttat atcatttgat tgtagactaa
     5701 acttcattta cttttttcct ttaactaata atttaaacgt aatgcgtatt caaaaatttg
     5761 attggctatg tagtcagttt ttatagcata tttaatatat cgaaacggat tttccatcgc
     5821 attttaatct aaaccatcag aattatttaa ttctcttcta atttgagcaa aatagtcact
     5881 tctgcgttta tttaatacac tcgaaagaaa tttttttgag aattcaaact gttttatcac
     5941 catcttttcc ttatccatac taataaaatc tataatagat cccgcaatcg cttgtgaaga
     6001 ggatggagta aatagacaat gtggccccag caactctgga attcctgctt tattagaccc
     6061 tatacaaggg caagcacggc tcatagcttc aattaccgca cgaggtaacc cttcttgtaa
     6121 acttggttgt atatataaat caactttatc caaatactca taaatttgtg atgtagtcaa
     6181 ttcccctaaa aaatggattc tattcgctac tcccaattcc tctgctttct tttttaagaa
     6241 actttgatca ccatcacctg ctaaataata atccaacaaa ataccttgta tttctaatct
     6301 cttcattgct tccaatacaa atctatggcc cttggctcgt acattcacag atgctgttgt
     6361 cattattgaa attttttgag gattaaattt tttcaactta taaagacggc tcttcaaaga
     6421 tctattctct acagaggtaa tattaacatt tgaagcaacg atattagatt taatattagg
     6481 atatctatct tgtaaatatt tttctgtcac gtatatgcaa taattagcgt tttttacaat
     6541 tgatttagtt ttggcgtata tatatggagc taataatttt ccctttaaac tatgatacca
     6601 gtaagaatcc caagcatccc ccattatttc tgtcagatac ggcttaccta tcttcaatgc
     6661 gtagtctgca gtttttgatc caattataga cggaatacga acaactatta agtcgctatc
     6721 aatcattcct tcaataatct tatttttatt ttgaccaaat agaacctgat gtagaccttc
     6781 tatagggata aatttattaa taaagttcgc tttatgatat ccttcagggt atgattccac
     6841 cttctcataa cgactacata gtacaatctc tccaaatgct ttagcatagc gctcataaat
     6901 aaaataagct ttcggtttta aatatatttc atttttatag attgtacatc taaattcagt
     6961 cgctataaat actttcataa tttcatctcc cttgataaac gtttctgaat aaatatagta
     7021 tcagctggaa tatcctttgt tacaacactt cctgctgcaa ccacactatt ctcaccaata
     7081 ctcactccct tcaagacaat gctgttagct ccaatccaaa cattttctcc aatcataact
     7141 ggggccgtct taaatttttt tgctttcaat ccacctggaa cactaacatc atagtcatga
     7201 tcatatataa gtactccagg accacatttt actccagatt taatatctat gtatttacga
     7261 caagctatca tacaattatt actcaaaaat acatcatcag aaatcttgag ttttgctcct
     7321 tttctaactt tcaatactcc cccggcgttt actctaatct tttttcctaa gattaattta
     7381 gcattagatt cctgttcgac agttacattc tttccaaatt ttaaaaaata atgaaaagta
     7441 agatccttgt ttcgacacac ttttaataat atcaagcaaa tatttgcacg aagtaaacca
     7501 acaatatttt ttataatcct attcatctaa agctcctttt attttatcta aataagcatc
     7561 tataataatt tgacgagaaa aatattgctc tatatacatt ctcccttgaa gtcccatctc
     7621 ttttcgtttt tcattttcta gacctaaaaa aacttccaat atagagacta gttcctcaac
     7681 atttccccct tgaaatatat agcctgtctc tctatcttta acaatttctt tgcaacctgg
     7741 attatctgtc gtaattaaaa cacgtcctga actagcgttt tctagtaata cattgctcat
     7801 tccttcacca tacacactgg gatggataat tgcatgggaa cgggtaatat gaggtatcac
     7861 atctttttgt tgtcccaaat aataaacgat tccttttttt tctaagtcac aaattttaag
     7921 ttcataatta ctctctgtcg gttctataaa gccaataata ttaaactctg ttttgggata
     7981 tcgactctta ataatttggg cagcagccag ataagtatct ataccttttt cttttaatat
     8041 cctaccaata aaattaaaaa cgattgtttc tccttgaatt ccatttccac catttggata
     8101 ctgttgcaca ggatactttt caatgttcac tcctgaccca ggtatcagct tagagtcacc
     8161 caaaatcaag ccaagagttt ttaacatttt catattatat tcattttgaa aaaaaacaca
     8221 atttgatttc ctaaaagaaa ttctaaacag taataacacc aggcttctta cacaagaacc
     8281 cattgttaag acactaccta aacctgttac gttatttata tattttattc ctaaaattgt
     8341 tgcagctagg ctagcatata catttggttt cacggtatat gtcaagacta catccggtct
     8401 atattttctc aataatctta tataactaag aaatagacga atatctttta aagggtttat
     8461 tcctcttcta tcaatagtaa tatcttcata ctcaaattga atagacttca tcaactctat
     8521 cttatctcca taaggacatg acactagcac ttcataccca tactcaacta gtgactctat
     8581 aacttctttt cgaaaacaat agatatcatc gtcatgatta gttattaaca taattttttt
     8641 cttttgtaaa ttcatcaaaa taatcttcct aaaatataat taaccgctac tccattatcc
     8701 tttacaacta tcattttaaa aatagtaatt ttctataagt aattgtttcc aaattgtgac
     8761 actttaaaat tctatacaca tatagaactt acaaactatt tacgaccgta aactccatac
     8821 ttatcaaccg agatatccaa tttatttaaa acaactccca ggaacagttc ccctgtttgt
     8881 tctaattgtt gtttcgcttt ttggacatca cgtttattcg cctcacctgt tgctgttatc
     8941 aagatggacg catcacactt ttgagtgata attgccgcat caataacaat tccaataggt
     9001 ggtgtatcaa caatgatata atcaaaatat ttacgcaatg tttcaatcat atcattaaaa
     9061 tttttacttt gtaacaaggc tgtagggttt ggtgatacag atcccgattg aactacaaat
     9121 aaattttcaa tatttgtatc acataaaccg tgagataaat cagctgtccc agataaaaat
     9181 tctgttagcc ctgtaatttt ttcacgagat ttaaaaaatc ctgacataac tgaatttcga
     9241 atatcgccat cgaacaaaag agttttatag cctgcacgcg caaatgacat tgctatattt
     9301 acggaagtag ttgtttttcc ttcccccgga ttaacagaag taacggaaat tacttttagt
     9361 ttatctccgc tcaactgtat atttgtacac aaggtattgt aatattcttc tgccttctta
     9421 atgaactcca gttttttttg tgctatttct aatgtcggca tctctctctc ctatttcaac
     9481 ttacccaagt ttggtacaac tcccaaaagt gtcatctgca gtgcattttc gatatcttcc
     9541 ggacgtttca cacgagtgtc caaaagttca agaagaagaa ctataacact agttccaatc
     9601 acccctgcca aaaaaccaat tagtgtattg cgtttaatat ttggcgaaga cggtgatatc
     9661 gccggtcttg cctcctccag tgttgtcaca tcagaaacac gagtaatact gataattttt
     9721 tgagcagcta cttctcttaa agagttagcg atacggcttg cctcttcagg aactcgatca
     9781 ctaactgaaa cagagacaat acgggtatca actggtactg ttactttaat tttattagcc
     9841 aaaccttttg gcatcaaatc tagtttcaaa tcagaaataa cttcctccaa aacatcctgc
     9901 gataggacaa tttcacgata gtctttaacc aagtatgctc ctgcctgcaa gtcttgattg
     9961 gttaaaccag gcttctctcc ctgattacgg ttaactagat aaatccgagt cgtactagta
    10021 aactcaggtt tgataacaaa agtactgtag gcaaaagcaa ctgaagaagt tataattgcc
    10081 actaataaaa tgaccaactt tcttttccat aaagctctga atagttgcaa tacatcgatt
    10141 tccaaagtgt tttgttcctt catattttct cctaaattaa ttgatccatt ataatttttc
    10201 tgggattatc tacaaaaagt tctttcgctt ttttcgctcc atatttctta gcaatgatat
    10261 catatgcctg ttgcatatat ggaggtctac tgtctaaatt gtgcatgtca cttgcaacta
    10321 catgaactaa atcacgttcc aaaaaatacc gagctctctt tttcatgaat ttatattttt
    10381 caccaaagaa cttaggtttt gaaacatgat aactatttat ctgagtatag caccccatat
    10441 caatcagttc acgaacacgt ttttcgttat tctctaaagc atcataacgt tcaatatgag
    10501 caattactgg cgtgattccc aacatcaaaa tattgctcaa tcccgtatga atctgacgat
    10561 aggaagtatg catgctaaac tcaatcaagg cataacgact atcattaagg gtaggaattt
    10621 cttttttttc tagcttttct agagcatcca gagtatagta tatctctgcg ccataagcaa
    10681 tgactaaatc atctgctact tcttttgcaa tttcccgaac ctgaagaaag ttttctgcta
    10741 tcttctcttc cggagtttca aacatgccct tgcgacggtg agaggtagag acaatggttc
    10801 tcaccccctg cctgtaggat tctgccaaga gagccttgct ttcctctctt gacttgggac
    10861 catcatctac atcaaagacg atgtgcgaat ggatgtctat cattttatct accctccatc
    10921 acatcctgta tagctgcttt aactacagtt aaactactat catctatttc catcacatag
    10981 aggttactgt ctggcattgc ataagaagga agatccatcc gacctgtccc ttttaaatct
    11041 tgagaattta ctttataatt ccctccactt tccaactgag tattcactaa atctatcata
    11101 gtctcaatcg gtatatttgt ttgaagagaa tcctgcaatc cttgaagaat actactatag
    11161 tttttcaaaa cctctgtaga agttaacttc tgaataattg ctacaatgac cttttgttgg
    11221 ttgcgaccac ggtcacggtc tccatcggtt agtgagtagc gttcacgtac aaaacctaaa
    11281 gcttgctcag agtctagatg gacattccct actgggaaat ggaacttccc atgtagagct
    11341 gaaaactctt gatcattatg aacatctacc cctcccaata agtcaatcat tttcaagaaa
    11401 gaagtgaagt tcaatcgcac atagtaattg atatccactc catagagatt ttctaaggtg
    11461 tgaatggacg aatcaactcc ataaatgccc gcatgggtta atttatcttt ttgattattt
    11521 ccactatctg cgattggtac ataggcatca cgtggcgttg tggtcaagag gattttcttg
    11581 gtatctcgat tgacagtcat taggatattg acatctgatc gcgacaccga actaatagga
    11641 ccataggtgt caattccact aacatagata ttgaaagact gattcttaga cgtcttagga
    11701 gcctctactt ttttagtgaa tcccttggta taaatctttt ttatcttcga tgcgtagtct
    11761 ggatactctg actcgatgat attttcaaag acactattta ggacaatggc cttagtctcc
    11821 cctgcaatca aactcttgta agctgccaag taagacgaac tctggttgac cgtcaaatcg
    11881 gtattctgac ttgacttgat atcagctagt agtttttgaa tattttcatt atcagtccca
    11941 gtcggtgctg tcacactcgt cagttgcgta acattttcga tcttactatc tgctaaaaca
    12001 gcgacactga ttgaatattc tgagtaatta gaagtcgcat ttaaacgatt ggtcagtcca
    12061 acaaactgct gtactgcaaa gagcgacact gagctgacaa ggatagagaa cagcaacaga
    12121 aaaatagtaa acttttcagc ttttttatag ataatcaaga gtagccctac caaggcaact
    12181 agtaggacta acgcagttac cactaggtta agatatctaa aagcaaggat attgtactta
    12241 aagattaaga acaataaaaa acaaattaac aataaataaa tagtcagcaa aacgatatta
    12301 acacttcgct tcactttctg tgaacgtgat tttttaaaac gtctactcat gattaacacc
    12361 tataccttga acatcataca attatatcac ttttttacgg taatgtctac acctttattt
    12421 tcactatctg catctttaag tatcttagta gacttcccgc gaaccaaaaa tatagtaaaa
    12481 tgaaata
//
LOCUS       DAVBHS010000049        12330 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVBHS010000049 DAVBHS010000000
VERSION     DAVBHS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12330
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.049"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(60..509)
                     /locus_tag="VQ651_001003"
     CDS             complement(60..509)
                     /locus_tag="VQ651_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET2754014.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(511..1269)
                     /locus_tag="VQ651_001004"
     CDS             complement(511..1269)
                     /locus_tag="VQ651_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET2754015.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1384..3363)
                     /gene="pknB"
                     /locus_tag="VQ651_001005"
     CDS             complement(1384..3363)
                     /gene="pknB"
                     /locus_tag="VQ651_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HET2754016.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3360..4100)
                     /locus_tag="VQ651_001006"
     CDS             complement(3360..4100)
                     /locus_tag="VQ651_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET2754017.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVFMNEEDAE"
     gene            complement(4115..5428)
                     /gene="rsmB"
                     /locus_tag="VQ651_001007"
     CDS             complement(4115..5428)
                     /gene="rsmB"
                     /locus_tag="VQ651_001007"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET2754018.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5421..6356)
                     /locus_tag="VQ651_001008"
     CDS             complement(5421..6356)
                     /locus_tag="VQ651_001008"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET2754019.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6369..8765)
                     /locus_tag="VQ651_001009"
     CDS             complement(6369..8765)
                     /locus_tag="VQ651_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET2754020.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGTVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(8831..9145)
                     /locus_tag="VQ651_001010"
     CDS             complement(8831..9145)
                     /locus_tag="VQ651_001010"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET2754021.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9170..9796)
                     /gene="gmk"
                     /locus_tag="VQ651_001011"
     CDS             complement(9170..9796)
                     /gene="gmk"
                     /locus_tag="VQ651_001011"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET2754022.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(9926..11539)
                     /locus_tag="VQ651_001012"
     CDS             complement(9926..11539)
                     /locus_tag="VQ651_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET2754023.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(11730..11984)
                     /locus_tag="VQ651_001013"
                     /pseudo
     CDS             complement(11730..11984)
                     /locus_tag="VQ651_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            complement(11988..12242)
                     /locus_tag="VQ651_001014"
     CDS             complement(11988..12242)
                     /locus_tag="VQ651_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HET2754024.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
BASE COUNT         3276 a         2943 c         2446 g         3665 t
ORIGIN      
        1 gaaagcgttg ttaatttatt tatctcattt ttcagaaaat tcttttattt ctgtaaagtc
       61 tacgatactc gatgtgtttt tatataatga tacagtctga gaatcactgt tccgctagcc
      121 attccaatag agattaccaa agccaacatg acaaccaagg tcgcacttgc cagtgcttta
      181 ttatagtccc ctgtcacaaa aaaggcagtt gttcggtagg agagataacc tggaaccagc
      241 ggtgccaaaa tggccaagat aaagaccaca gcaggtgtct tataaagaat acttaaaatc
      301 tggctgacac aagaaccaat aatggctgca atgaaggtag ctacaatgac attggtcggt
      361 tccttgagca agagatagat tagccagaca gtcatgccca aaatccctcc aggtaagagc
      421 atagaccgtt gcacattgag tacgattaaa aaagtgataa tggcaagaaa acttgctact
      481 gcttgtaata aaaaggttgt tagtgtcata ttagttcatc aataccaagg cgacagaagt
      541 tcctgcccct aaagcgaggg taatgagcag ggattcaaac atcttactca taccagagtt
      601 tatgtggttg gtcataatat cacggaccgc attggtcaag gcaatacctg gtacaaacgg
      661 catgaccgca ccagctataa tcaaatctgc cgttgaagga aaacctgtgt agcgagccca
      721 aaactgggca attatcccaa agacaaaggc tccagcaaag gctgtcacaa agggaattcg
      781 gataaatttt tccacataga gggaaaaggc aaaaccaaat aaggtcgcca ctcctgcccc
      841 aagtgcgtcg tagatatttc cgctaaacat aactgaaaag aaaggagcac taaaggtcgc
      901 agccagagtt acctgcaact tagtataggg aaggggttga gcttgcaagg ccgtcaattg
      961 cttaaaggct gtttctaagt caatctgccc cccaactagc tgacgagaaa tctggttcac
     1021 atcgcagact ttttcgatgt tataagaaga ggaggtcacg cgcttcatgc gcgaaatatt
     1081 ggtattttca atagagaaaa agatagcggc aggcatggca aggacattgc aatccacaat
     1141 cccctgcgaa tgcgcgattc gaatcatggt atcttctaca cgatggattt ctgagccact
     1201 tttaaggaga atagtccccg ctagcataat cacatcaatg acggcattta attcttttga
     1261 ttcttccatg ctttcctcct tttatcaact ccctctattc tatcacaaat ccggactcaa
     1321 aaaaaatctt tgccatgaaa tcatgacaaa gattgattac tcattttgat tatccatctg
     1381 cttttaagga gtagctgaag ttgttttagg tttgtagatt gaaatcttga ctctagtctt
     1441 attgaggtct accttttcac ctgctctagg actttgttca acaaccatgc cttctacact
     1501 acctgcaggc gctgtcgtca cttctacaac ttctatatta gcttccttaa tcccaacaat
     1561 ttgaatcaaa ttgttcttag taaactccaa gctggaacca atgtaactcg gcatggcaac
     1621 acttgtaact tttttagcta ctgtcaagac aatttgagta ggtttactca catcataagt
     1681 cgttccggca cctggacttt gtttcataat cgttcctggt tcgctttcgc tggactcttc
     1741 ttcctctatc ttaatcaaat tctcaggaac cttcttctgc ttgagttctg agattacttc
     1801 tgtagagttc cgtccaatat agttccctaa ttgaatcgtc gtagcttttt tagctactgt
     1861 caaaacaatt tgagttgcct tgctcaagtc ataggtcgta ccttctggta gactttgctt
     1921 caggaccgtt ccagcctcac tctcattcga ctcttcttcc tcaattttaa tcaaattatc
     1981 tggaactttt ttctctttta attccgcgat aacatcagaa gatttccggc cgacataatt
     2041 actaatttgg aaggattgtt tgcctgatga gacaaccaga ttgattttcg ttccttcttt
     2101 tcgaccagtt ccagcgccag gatctgtacg gataatccgc ccttcttcca ccttttcact
     2161 agcctctgtc ttctcctcac caatctcaaa attggctttt ttgagcgttg ccttggcctc
     2221 tgcaactgtc tgacctgcca catctggaat ggcaatggtt gcaggagttc tggatagtat
     2281 ccaaataaga gaagctgcca ccaatacaag gctggccaac aaaatcaggt aacgcatctt
     2341 aaatctatgt tttttcggtg cttgtggttg gtaagtttcc tctgtcacag cctggcttgg
     2401 gtttttgatt gatttgtgtt ctgtctgcgc ttgaacctta ggaatagatg tcaaggtact
     2461 ctgagaaacc ttcggcaagg tcttggtatc tgccttgctc gtttcatcaa agattaactt
     2521 actttcattt ctacgattgt aggacaagct actagacaag tccacataca tctctgaaac
     2581 cgagcggtag cgattggtca actttttagc agttgccttg ataataacat tttctaaagc
     2641 ctgaggtaca gatggatttt ctgcaataac ggacggcagg ggtttctgga aatgctggag
     2701 ggcaatggtc accgcgctat ccccgtcata agggatatgg cctgtcaaca tctcatagaa
     2761 aataatcccc atggcataga tatcactctg cacagtcgcc ttcgaaccac gcgcctgctc
     2821 tggtgacaag taatgaactg agcccaacat cgagttagtc tgggtcagac ttgtctctgc
     2881 aaaggctaca gcaatcccaa agtctgtgac cttggcagtc ccatctggtg tcaaaaggat
     2941 attttgaggt ttcaagtccc tgtgaacaat tcctcgagta tgggccaagc gcatagccaa
     3001 gagaatttgt cccatgatac ggactgcttc ttcattagaa agaggataat gttccttgat
     3061 atagcgtttg aggtccagtc cagccacata ctccatagct aggtactgtt gaccgtcttc
     3121 ctcgccaata tctgttatcc gaacgatatg aggatggtct agatctgcca tagctctcgc
     3181 ttcacgctga aaacgagcta cagctatcgg gtccgtctgg tagttggtcc tcagaacctt
     3241 cactgccact tcttccccat ctaagattaa gtctttggct aggtagacat ccgccatgcc
     3301 tcctcgacca atctgtttga caatccgata gcgtccggca aaaatcttgc cgatttggat
     3361 cattctgcat cctcctcgtt cataaaaaca agggcaaccg taatgttgtc taaacctcct
     3421 gcattgttag caaaacgaac aagtgtctcc gttttatctg ctaaaggaat atcactggtt
     3481 acaatatcac gaatctcact gcctgaaatc atgttggtca agccgtcact attgagcaag
     3541 agatagtcac ctgactcaag gataactgtc ccaaaatcag gctgaatttc atctttttgc
     3601 ccaatagact gggtgataat atttttttgc ggatgagctt ctgcctcttc tggtgtcaat
     3661 tgaccagcct tgagcaattc attaaccaag gaatgatcgc tcgtcaactg atggtattct
     3721 tctccacgaa tcaagccgat acgcgaatca ccaatatgag catagatagc ctgattatca
     3781 ataatagcaa ggacttccaa agtagttccc atgcctctgt aagcttcatc ctgaccaagc
     3841 tggtgaatct tttgattttc aatttctagg taatgggcga accattcacg cacttcattg
     3901 actgtatcga tctgggtatc aacccaagct acacccaggt ctgtgaccgc catttcacta
     3961 gcgatattcc ctgcacgatg acctcccatc ccatcagcta aaataatcat ggtacgtcca
     4021 gctctattga catagtggtt gacatagtct tggttatttg ttcgtttctg accaacatct
     4081 gttaataatg aaatttccat gtgtcagttc cttcctaatc cgatatcttg cgaaattgac
     4141 tgatgaagaa tccatcactt ccatacaatt caggtgtaat gaggatacag ccgtctttca
     4201 tgatatcctt acattcatgt tctagtttta cctgctcgaa ctcgggatga ctctctaaaa
     4261 aggccttaac gacttgaaaa ttctcctctg agacgatagt gcaggtgcta taagttatta
     4321 taccaccttt gcctagtatt tgacaaacac tacctaatat ttctaactga atttcctgca
     4381 aggacgcgaa atctgccgtt tctttattgt atttgatatc tggttttcgg cgcaaaagac
     4441 cgattcctga acaaggagca tccaccaaaa tcttatcaaa ggaatcctgg tcaaaaaact
     4501 catgcacctt tctggcatcc aatttttgag tttgaacccg atcttcaact cccagacgtt
     4561 gggcattttc ttgaattaaa tccaacttgt ggtcgtacaa gtccagagca gtaacctgac
     4621 ctgtcgtaag ataagaggct atatgggctg ttttcccacc tggagccgca caggcatcaa
     4681 gcactcgctc atcaccttgt aaatcaagcg tcggagcaac caactgactg gactcgtctt
     4741 ggatggtaat ggctccatcc gcaaacaaat tatgccctgc aaaatgcccc tgctccttaa
     4801 ccagaccagt ggttgctaaa agggaattat tcgcctccaa caaggcttgg atttcctctt
     4861 ttcgacttag gtctgttaca cgaatactgg ctttgtttcg cactaacagg ctttcaaaga
     4921 tggcttttgc tctctcctct ccgtattctt ccttgagttt ggcaactagc caaactggga
     4981 gagaataggc aatggagtca cgcttgtttt ttcgcttgat gctagcaata tctggccagc
     5041 cttcacgcaa gatacggcga aggacagcgt tgaccaattt ttcactgcct tttttacgga
     5101 gtttggccaa ttccactgct tcattgacca cagcatgatc tggaatcttg tccaaatagc
     5161 ggagttggta ggcactcatg agaagaagga catagagcca gctgtctaac tggtctctgt
     5221 cttcgataaa gtgggatagg taccattcca gagtcagttt acgggctacc gttccataga
     5281 ctagctcggt cactaagccc ttgtctgctg cagagagctg actccccttg aggtgtttat
     5341 ttaaggcaat atttgaatat gcttgattca caaaaacatc ctctagcact gctagagcta
     5401 aacttctagc cgtttctact ttagtcacca aatcgttctc ctacagtcaa tgtacgtcca
     5461 actccattga ggaaggaagc aatgtccatc ttaggcttac cagctggctg cacttgtttg
     5521 agggatagag ccccttcagc cgttgcgaca atcaattctt tcttgccgat agagagaatc
     5581 tcacctggat ttccctgacc ttctactggt agggcttcat aaatcttaaa gcggtcgccc
     5641 ttaaggaaag tatgggcaac aggccagggg ttcattccac gaatttggtt aaagagttga
     5701 cgattggttt tgttccagtc cagtttttct tcctctggct ttatatttgg agagaaggta
     5761 acctgactcg tatcctgcgg ttcaggtttg atatcaccag caatataggc aggcagagtg
     5821 tccaaaagca aatcacgacc aactagcgcc aatttttcaa acaaggtgcc aacattgtcc
     5881 tcatctgtga tcggaatgct gcgacgagaa atcatatctc ctgcatccat ttccttaacc
     5941 atttccatga tggtcacacc agcttcctca tccccttgaa tcaaggcata atggataggc
     6001 gcaccaccac ggtgtctagg aaggagggag gcatgaacgt tgacagcaaa gtccatgcta
     6061 tcaaggagtt tgcttgggag aaactgccca aaagcagcag tcacaattcc atctgctcct
     6121 agcttcataa gttcttccat ctctggactt ccagataatt tttcaggttg gtagatagat
     6181 agtcctgctt ccttggcagc ctgcttgact ggggtttctt ggataacttt tttacgacca
     6241 acagcacggt ctggctgggt cacaacggct agaatttcgt aacggtcatc tgtcaaaagt
     6301 ccttttaaga ctgttgctga aaagtcgggg gtccccataa agattagttt tgtcatatct
     6361 tctccttctt ataaaaattg ctgcggctca tggtcaatgc tgagacggag ctcactattt
     6421 tcccgttctt gagtcaaggc caagacctgg ttgagggtcg gccccagctc atcttctaaa
     6481 cggtatttaa ttaaaatctg gtaatgatag aggttgtggg tacgggcaat cggttttggc
     6541 gttggcccca gaatgggact ggtctctgac aagcctgacc gcaaaatgtt catgacttca
     6601 taggcacgtt tgaaaacctc ttcttctttc ttgtgagaaa gggtaatacc aatcgtgaaa
     6661 tagtaaggtg gatagccgag ttgtcgtctg attcccattt cataggcata aaagccttcg
     6721 taatcttgat ccttggcaaa tcgaatagca tagtgctgcg aattgtagga ctgtatcaag
     6781 acttgacctg ctttttcagc ccgacctgct cgacctgcca cctgagtcaa gagctggaag
     6841 gttctctcag aagaacggaa atcaggcaga ttcaaggccg tatccgcatt tagaactccg
     6901 actagggtaa cattgggaaa atccaaaccc tttgcaatca tctgagtacc aagtaaaata
     6961 tccgcttccc ctcgcccaaa ctggtcaagc aaggcttggt gactgccttt ctttcgagtc
     7021 gtatccacat ccatcctcaa aatgcgagct tggggaaaga gttctgctag ctcatcataa
     7081 gccttctgag ttcccgtccc atagtaacga atactgcggc tcttacagtt aggacagacc
     7141 tgaggaatat ccttcgagaa accacaataa tggcagttca tagtcttggt atccatatgc
     7201 aaggtcagag aaatatcgca gttgggacaa gtatccaccg tcccacactc ccgacacatg
     7261 acaaagctag aataaccacg gcgattgagc atgagaacca cctgctcttt tttaaccaga
     7321 cggtcttgga tagcctctag caaaggaggc gtaaagtttg acgtctcatt ttgtccgata
     7381 tagtctcgaa agtcaatcac ttgaacctca gggattgtag ccaaaggatt ggcacgttgg
     7441 gttagacgta agtgttgata gacgcctttg ccagcacgtg cccggctctc taagctcggc
     7501 gttgcagatc caagtaccag agttgcttga ttatactgag cccgtaaaat agctacctct
     7561 ctggcatggt aacggggatt gctgtcctgc ttataagccg cttcatgctc ttcatcaata
     7621 atcatgacac ccagattttt cagaggagca aagatagcag atctggcacc aacaacaact
     7681 tgggcatcgc cacgctccac cttgcgccat tcatcatact tttcgccatt ggataatcct
     7741 gagtgaagaa tggctacctt gtccccaaaa cgtgctataa aacgctcggt catctgagga
     7801 gtcaaggaaa tctcaggtac cagcaaaata gctgtcttac ccttatccag ggcaccttgg
     7861 ataatctgca agtaaacctc ggtcttccca cttcctgtaa tcccttgaag tagaaaggga
     7921 ggttgagaac tgccaataga actcacaacc gcatcacgcg cctgtctttg ttctggattt
     7981 aactccaaag gtctacttgc ttcaattcct tcaaaataag cagccgagcg ttgaacttcc
     8041 ttttggacta tggtaacagt accttgatcc acaaagaagt tgacttgctc tcgcgagtag
     8101 gactctaaca agctagccaa ggaagcgctc tctggatgag acagcagata atctctcagt
     8161 tccaactttt tcttggcacg tgtagaaatc tcaacacctt ctaattgggc atggtcaacc
     8221 tcataccaag actgggtctt gaccttcttt tgatcgactg cctgatattc cagaccaagc
     8281 aggccttttc tagtcaaacg catcatttca gcttgcttgg caaggtctag tgaagaaaag
     8341 gctagcgaat cttctgaacc aaacaggcgt actcgttctt cctgactcaa gccttccaga
     8401 ggatagagaa tcttgtcata gctagaattc agaaaccctg gaagcatggc cttgaggata
     8461 gagattttgt aggagaagac agatttgcgt aactcctcag ccagccagag ttgttctggc
     8521 gtgagaacag gagaaaaatc cagcacctct gcaatatctt ttaaatcttg ctccatctct
     8581 tctccatctg attgggactt caaaccaaga acaatccctt gaatcaggcg attaccctta
     8641 ccaaaaggca catgaacccg catcccaact tccagcattc cctcaaattc ctccggaatc
     8701 ctgtaactat agggctggtc cgtctgcatc aagggcacat ctacgataat cttagctagg
     8761 gccatcttct cacctcctcc ttgtcagtac attcttgcaa tagaaaaaat aagattgagt
     8821 ccccccaacc ttaaattttt tcaccatctt ctttttcttt agcaatttgc tctttgattt
     8881 tcttttcttc ttcttctttg cggcgttttt cttcttcgat acggcgacgc actgcttcac
     8941 gttttccttc tggatctggg tgaattgtaa cgtttcctga ttcgatttct tctaaagcgc
     9001 gaagagttga tttttcagac ttgaaacctt gagttgctgg ggcacctgct tccaattcgt
     9061 gggcacgttt tgcttccaag attacgagtg aatattttga aggaaccttg tcgagcaagg
     9121 tatcaataga gggttttaac atcatttgct tgtacctatt ttctaaattt tatcgggtag
     9181 ttggagattt tggtaacatc tcctgatagt gaccaatgac acgatccaca cagaagtgtt
     9241 ctgcttcaat cacacatttg acacgttcag cagctagggg cacctgatcg ttgacaatcg
     9301 cataatcata ctcacgcatg agggcaattt cttccttggc cttttcgatt cgttgggcaa
     9361 tcacttctgc actatctgtt ccacgaccta ccaagcgatc ttgcaattca tccaaatctg
     9421 gtggtgtcag gaagataaag acagcatctg gaaccttttt cttgacctga agagcaccct
     9481 gaacttcaat ttcaaggaaa acatcgattc ccttgtccaa ggtttcattg acataggtca
     9541 gaggagttcc atagtagttg ccgacatatt ctgcgtattc caacatctgt ccttgacgaa
     9601 tcagctcttc aaattcttca cgagtacgga agaaatagtc aacaccgtcc acttctccag
     9661 gacgttgtgc gcgtgtcgtc atcgatacag aatattgaaa ttggttttca gaactctcaa
     9721 aaatctctct tctaaccgtt ccttttccaa cccctgaagg accagaaaaa acgattagta
     9781 agcctcggtc tgccattgtg tctcctttta gtcaatctgt gaaataacat ttctctagaa
     9841 taatggcaaa aagccagatt atcctttaca gtctttctat ctagtgtaac aaaaaagcag
     9901 taatttttca actgctcttt cttatttatt tagcataatc tactgcacga agctcgcgaa
     9961 tcacggttac cttgatattt cctggataat cgagattgtt ttcaattttc ttacgaactt
    10021 tgtgagccaa gattgtgact ttgtcgtcct tgatttttcc tggattgacc atgatacgaa
    10081 tttcacgtcc tgcttgaagg gcaaagctag tttgcactcc ttcaaagccg ttagcaattt
    10141 cttccaaatc atggagacgc ttgatgtagc tttcaagaga ctcactacga gcacctggac
    10201 gggctgcgct caaggcatct gctgcagcga cgataactgc tatcacgctc tcagcttcaa
    10261 catctccgtg gtgactagca atcgtattca ccacaactgg gggttccttg tacttacggg
    10321 ccaattccat accgatttca acgtggctac cttcaacctc atggtcaatg gctttcccga
    10381 tatcgtgaag gaatccagca cgacgggcaa gagccgcatt ttcaccaagt tcgctcgcca
    10441 tgataccagc caacttagca acctcaatcg aatggcgcaa aacattttgt ccatatgaag
    10501 tacggaactg caaacgtccc ataatcttca tcaagtctgg atgaaggttt ggcgcaccaa
    10561 tttcataggc agcagcctca ccgtattcac gaatcttatt gtcaatctct tgacggtttt
    10621 tctcaaccaa ctcttcgata cgagctggat gtatacgacc atctttgagc aacatttcca
    10681 tagtcatacg ggcaatctca cgacgaatcg gatcaaatcc tgacaaggtc accacttctg
    10741 gtgtatcgtc gataatcaca tcgacccctg tcaaactttc aaaggtacga atgttacgac
    10801 cttcacgacc aataatgcgt cccttcatag tatcgtctgg cagatgaact gttgagtttg
    10861 ttgactccgc tacatattca ccagcgatac gttgcatagc ttgaaccaag atgtccttgg
    10921 ccattttgtc agaacgttcc ttgacctctt gctcagcttc gcgaatgcga ctggcaatct
    10981 ccctggtcaa gttttcctct gtctgagcca agataatatc tcgtgcttct gcctgagaca
    11041 gagcaccaat acgctctagt tctgcttctt tttgtctttc gacttcctct aattgctctt
    11101 cacgcgcatc aaggtttttc gctctatcag aaatactttg ttctttttgt tcaagtgttt
    11161 gttctttact cgtcaaattg tcgtccttac ggtcaaggct agtagctctc tctgtcaaac
    11221 gactttcgat ttgtttgagt tcttgacgtt ctgatttgaa ttcagcgtcc acttcttcac
    11281 ggtattttct ggcttcttct ttggcctcca atagtgcttc ttttttaaga gacttgcttt
    11341 cacgtttggc ttcattaaca agtaaatccg cttcacgctc agcttgtcca cgtaaattag
    11401 ttgcttcttg ttcagcattt aaaagcatca actctgcagc ttcctgagat gatttcatct
    11461 tagctgagat gctgacatat ccaatgacta aaccaatgat gacggcaaaa acagcaatcg
    11521 caagcgacat gatttccatg tttttacctc attttattgt tattccgaat gacatacatt
    11581 cttttacatt ctaccataaa aaagtgattt tcacaaacct aaaatagaat atgttttggg
    11641 gaatttggaa cacatttacc aaaataaact tgttgtttag aaatagtagt ttagtagaga
    11701 cttgagaaaa agcctacttt gcagtagact tagtaatgat ctttaaagga caagaaagcc
    11761 acgctatctc catccatcat ataaatcaag cgattttctg catcaatacg ccgtgaccag
    11821 gctccttggt aatcatattt gagtggctct ggttttccta ttcctgtaaa tggatcgcgt
    11881 tgaatatcct tgattaattt attgattctt tttagtgttt ttttatcttg gttttgtcag
    11941 tagcaataat ctgcccaggc atcttctgta aacttgagta gcattcatca ctcctcaatc
    12001 acatggacct gagtgcttcc agcacggact tgagccattc ctcgcaaaac cttgtctgat
    12061 aactccttat tttgggcgat tctcagggtt tcctgaatac tgtcccattc gctctttgaa
    12121 agaactacaa tgtcctcatc tggattttta ttgaccaccg tcaaaggctc aaattcatca
    12181 tttaccttct tcatgtagtc ctttaaatga tttcggaatg ttgagtaaag gactgcttcc
    12241 ataaccatac ctcgttttag ctcttttcca ctattataca cgaaaagaaa gaaattgtca
    12301 ggaacttgta caagattttc ttttctatct
//
LOCUS       DAVBHS010000050        12109 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVBHS010000050 DAVBHS010000000
VERSION     DAVBHS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12109
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.050"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(116..2374)
                     /locus_tag="VQ651_001015"
     CDS             complement(116..2374)
                     /locus_tag="VQ651_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2754025.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2603..2833)
                     /locus_tag="VQ651_001016"
     CDS             complement(2603..2833)
                     /locus_tag="VQ651_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HET2754026.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3023..3706
                     /locus_tag="VQ651_001017"
     CDS             3023..3706
                     /locus_tag="VQ651_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2754027.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSEIVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3706..4440
                     /locus_tag="VQ651_001018"
     CDS             3706..4440
                     /locus_tag="VQ651_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2754028.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDIAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4594..5451
                     /locus_tag="VQ651_001019"
     CDS             4594..5451
                     /locus_tag="VQ651_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HET2754029.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGYRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5593..6465
                     /locus_tag="VQ651_001020"
     CDS             5593..6465
                     /locus_tag="VQ651_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET2754030.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLMTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVILLGPGL
                     GDNAFGEDLVKQVFAGLKQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            6747..7430
                     /gene="rpiA"
                     /locus_tag="VQ651_001021"
     CDS             6747..7430
                     /gene="rpiA"
                     /locus_tag="VQ651_001021"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET2754031.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7444..8655
                     /locus_tag="VQ651_001022"
     CDS             7444..8655
                     /locus_tag="VQ651_001022"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HET2754032.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8657..9199
                     /locus_tag="VQ651_001023"
     CDS             8657..9199
                     /locus_tag="VQ651_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET2754033.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9215..10024
                     /locus_tag="VQ651_001024"
     CDS             9215..10024
                     /locus_tag="VQ651_001024"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET2754034.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10500..11480
                     /locus_tag="VQ651_001025"
                     /pseudo
     CDS             10500..11480
                     /locus_tag="VQ651_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            11505..12017
                     /locus_tag="VQ651_001026"
     CDS             11505..12017
                     /locus_tag="VQ651_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HET2754035.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPISAQPHRKITHWGTTL
                     NSKVSDDDVL"
BASE COUNT         3570 a         2218 c         2752 g         3569 t
ORIGIN      
        1 agattcagtc tactatatat cataatggac aaagggcaag ctgtcaagaa attacagtta
       61 ttttagtaaa aaaagaagca gaaaaattta attttctact ccttttctat aggatttatt
      121 tttctttttc agaactttta acttcagctt ttttggcaga tttaatctga atgtttccct
      181 tgctagtcat aactgctttg agatcttttt cgcttggatt ttcaaggtag tagtcagtga
      241 ttgtgtcctc aatatagtct tgaatagtcc gacgaagtgg gcgtgctccc atttttggat
      301 cataacctag gtcaaccaac ttttccttga ccttgtcagt tacatccaaa cgaatgttgt
      361 tgctagagag gcgcttgtta acatctgcta gcatgagctc gacaatctga aggaggttat
      421 ccttgctgag agccttaaat tcgataatgc catcaaaacg gttcataaac tctgggctaa
      481 agaagttacc gagttcaccg aggacagaat tggtacgtcc ttctctagca gcaccaaatc
      541 caacgctagc ttcggtcttt cctgtacctg catttgaggt catgataatg atggcatcct
      601 tgaagctaac ggtgcgtcct tgcccgtctg tcaaacgacc atcgtccaag acttgaagaa
      661 acatgtgcat aacatctggg tgagcttttt ccacttcatc gagaaggatg agagaatatg
      721 gattgtggcg aactttttca gttaattgac cagcctcatc atagccaaca taacctggag
      781 gagcgccgac caacttagcc acactatgtt tttccatgta ttcactcata tcaaagcgaa
      841 tcatactatc agcagaacca aaaagttcga tagccagttg tttggaaagt tctgtcttac
      901 cgacaccagt tggcccaacg aagaggaagc ttccgattgg gcggttaggg gtaccaagtc
      961 cgacacgatt acggcgaata gccttggcaa tcttatcgac tgcatcatct tggccaataa
     1021 catgagactt gagatcttcg gctagatgga tgagttgaga ttgttctttc tctttcaaat
     1081 caccaacagg gatattggtt ttctgctcga taatgtgctc aatagttttc tcgctgatga
     1141 taggagtatc ctggtctgtg atctttttct tttgcatttc cttatacttg gcaatctggt
     1201 cgcggaagta ggccgccttc tcaaaatctt cttctcgtgt agcttgagac ttgagatttt
     1261 cagcctcaat caagcgctga tcaattactt taggatccac aaaattcaag gtcaagttca
     1321 tcttagaacc agcttcatct aggaggtcaa tggccttgtc aggcaagaag cgatcttgga
     1381 tgtagcgatt ggaaagagtt gcagctgctt caatcgcagc atctgtatat tgaacgtggt
     1441 ggtaatcttc gtatttcttt tgaatccctt tgagaatagt gattgtttcg tccaccgttg
     1501 gttcatcgac tttaacaggc tgcatacgac gctcgagggc agcatccttt tcaatgatac
     1561 ggtattcatt gagggtagta gcaccgacta gttgcagttc tccacgagca agggctggct
     1621 tgaggatatt tcctgcgtcc atattaccat cactcgcaga accagcacca acaatttcat
     1681 ggatttcatc gataaagagg atgatgtctt cacgtttgcg aatttcttcc atgagttttt
     1741 gcatgcgttc ttcaaattgt cctcgaatcc ccgttccttg aactaagcta accacatcca
     1801 gacggatgac ttgtttacct tggagtttat gtggcacatc gccatcgaca attttctgag
     1861 ctagaccttc gacaacggcc gtttttccga caccaggttc accgataagg acaggattat
     1921 tcttggttct acgattgaga atctcgatga cacggataat ctcatcgtcg cgcccaataa
     1981 cggggtcaat gtctccacga cgggcaattt cagttacatt aataccaaat tcttccagca
     2041 ggcctttttc ttggctaggt ggcggagttt gagcagatcc acgattttgg gaaccataac
     2101 cgccgtttcc accgtaacct ccacctgatt gggttggggg aataggagga gtattgctag
     2161 aaggtctgaa attgtttaga tcattgaaga aatcaccaaa gggatcgaag tcacgattgt
     2221 tcagatccgt catacctttg aagaggctat tgttaggatc tgttttgata atcttatagc
     2281 agttttgaca gaggtcaatt tgtttttgtt ttccattgag attggtgtaa agatgaattg
     2341 ttgagtcgtt aattttacag ttttgacaaa gcatacatct acctcatttc tttctttagc
     2401 ctgacttgtt taaaggtcaa aaatagtcaa attctatact ctcattataa caggattggg
     2461 gtgtaaagca agtaaaaaga ttgaagcttt gagaagttgt tacaatgaaa atttttgtga
     2521 atttggacta taaaaaaatc ctatttgtga gacaaaaagg attttactta gacatgcagg
     2581 gacaatccca gcttgtttta gattagtaga gaagttcgta aatttccatt gcgattaagt
     2641 caatgctatc aaacgtatac gtttcgcgat cttctacgtc acgaagggta aagattgggc
     2701 cgttttcttc gtcgtggctg tacgcaactt ctgcaacaac aactccttcg cgttcaaaat
     2761 tacgttttaa atttccgcca tcgtttgcca ttgcttcaag gcggttgatg attctaacca
     2821 aatgtgattc cattttgatt ctccttcatt tcttatcgtt actattttac cataaaggag
     2881 gccaacttga ctagaaaaaa ctaagatttc tcgctaattc tgctagttta aagattttta
     2941 aataaattgg ataaaaaatt taaattttaa ttcaaaacat gttataatca tacagtattc
     3001 tattttagaa agcagtgtga ctatgaattt ttctttttta cctaagtatt taccttattt
     3061 taactatggg gctgttgtga cgattcttat ttctatctgt gttatctttt tgggaactat
     3121 tttgggtgtt gtcttggctt ttgggcaacg ttcaaagttt aaaccgcttg tttggttggc
     3181 caacttgtac gtttggattt tccgtgggac accgatgatg gttcaaatta tgattgcctt
     3241 tgctcttatg catatcaatg ctccgactat tcagattgga attttaggtg ttgatttttc
     3301 gcgtctgatt ccagggattt tgattatctc tatgaatagt ggtgcttatg tttcggagat
     3361 tgttcgtgcc ggaatcaatg cggttccaaa aggtcagcta gaggcggctt attcgctagg
     3421 gattcgtcct aaaaatgcga tgcgttatgt gattttgcca caagcagtca aaaatatctt
     3481 gccagcattg gggaacgaat ttatcaccat tatcaaggac agctccctct tatcagctat
     3541 tggggtcatg gagttgtgga atggggctac aacagtttct acaacaacct atctaccttt
     3601 aacaccactt ttatttgcag cattttacta cttgattatg acctctattc tgacagtagc
     3661 cttgaaagct tttgaaaaac atatgggaca aggagataag aaataatgac agaaaccttg
     3721 ataaaaattg aaaatttaca taaatctttt ggaaagaatg aagtattgaa gggcatcaac
     3781 ctcgagatta aaagaggaga agttgtcgtt atcatcggtc cttcagggag tgggaaatct
     3841 accttgcttc gctctatgaa tttgttggag gaagcaacca aggggaaggt tatctttgag
     3901 ggagtcgata ttacggacaa gaagaatgac ctgtttgcca tgcgtgagaa gatgggcatg
     3961 gttttccaac aatttaacct ctttcctaat atgactgtga tggaaaatat taccttgtca
     4021 cctatcaaga ctaaaggtga aagcagggaa gttgctgaga agagagccca agagcttttg
     4081 gaaaaagttg gtttgccaga taaggcagac gcttatccac agagtctgtc gggtgggcaa
     4141 caacaacgga ttgccatcgc gcgtgggttg gctatggaac cagatgtttt gctctttgac
     4201 gaaccaactt cagccttgga tcctgaaatg gtaggtgagg tattggctgt tatgcaagac
     4261 attgccaagt cagggatgac tatggttatc gtaacacatg agatgggatt tgcccgtgag
     4321 gtggcagatc gtgttatctt tatggcagac ggtgtggttg ttgaagatgg gacacctgag
     4381 cagatttttg aacaaaccca agaacaacgg actaaagatt tcttgagtaa ggttttataa
     4441 tctattttaa aatttcaaga caagattagt gaaaagttca accagagctt tttcttatag
     4501 tttgaaacta taggataacc taggaaagaa gtgttagagg gcactagcag tttttggtat
     4561 aatggaagat atttgaaaga aaagagaagt gatatgacac agattattga tgggaaagct
     4621 ttagcggcca aattgcaggg gcagttggct gaaaagactg caaaattaaa ggaggaaaca
     4681 gctctagtgc ctggtttggt agtgattttg gttggggata atccagccag ccaagtctac
     4741 gttcgcaaca aggagaggtc agctcttgcg gctggttacc gtagcgaagt agtgcgagtt
     4801 ccagagacca ttactcaaga ggaattgtta gacctgattg ctaaatataa tcaggatcca
     4861 gcttggcatg ggattttggt ccagttgcca ttaccaaaac atatcgatga agaggcggtt
     4921 ttattagcca ttgacccaga aaaggatgtg gatggtttcc atcccctaaa catgggacgt
     4981 ctttggtctg gacatccagt catgattccc tcgacacctg caggaattat ggaaatgttt
     5041 catgaatatg ggattgactt ggaaggtaaa aatgcggtcg taatcggtcg ttcaaatatc
     5101 gttggaaaac ctatggctca gcttcttttg gctaaaaatg cgacagtaac cttgacccac
     5161 tcacgaactc ataatcttgc caaggtggct gctaaagcag atattcttgt agtcgcaatc
     5221 ggccgcgcca agtttgtgac tgctgacttt gtcaaaccag gtgcggttgt cattgacgtt
     5281 gggatgaatc gagatgaaaa tgggaagctc tgtggagatg ttgattatga ggcagttgca
     5341 ccacttgcta gccatatcac gccagtccct ggaggtgtcg gtcctatgac cattaccatg
     5401 ctgatggagc aaacttatca agcagcactt cggacattgg atagaaaata agagaagcat
     5461 tcgattaata gagtgtattt tctatagcta tatctaaaat ggcagaaatg aatattaaat
     5521 tttagatata agattacaaa aggaggtctg cgtctccttt ttattgtata ataaaagtga
     5581 gaggaaaaaa ggatgaaagt gattaatcaa accttattag aaaaagttat tattgaacgt
     5641 tctcgttcaa gtcataaagg agattatggt cgtctgctgc tgcttggtgg tacttatcct
     5701 tatggaggtg ccatcatcat ggctgcttta gcagctgtaa aaagcggtgc aggattgatg
     5761 accgttggaa cggacaggga aaatatcccg gctctgcaca gccatttacc tgagcctatg
     5821 gccttttctc ttcaagacca gcaattgtta aaagagcaat tggagaaggc agaagttatc
     5881 ttgctggggc ctggtttagg agacaatgct tttggagaag atttagttaa acaggtcttt
     5941 gctggcttaa aacagaatca gattttgatt gtagacggag gtgccttgac cattcttgct
     6001 aggacaagtt tgtcatttcc ctcaagccag cttatcctaa ctcctcacca aaaagaatgg
     6061 gaaaaattat ctggaattac gattgaaaag caaaaggaag atgcaacggc tagtgttctg
     6121 acttccttcc ctcaaggaac aattttggtg gagaaaggcc cagctactcg tatttgggaa
     6181 gttggccaat ctgattatta ccagttacag gttggtggtc cttatcaggc gactggggga
     6241 atgggagata ctctggctgg gatgatcgca ggatttgtag gccaattccg acaggccagt
     6301 ctctacgagc gtgtggcagt agcgacccat cttcattcag ccatagccca agaactatct
     6361 caagaaaatt atgtggtctt accgacggaa atcagcaggt atcttcccaa aataatgaaa
     6421 attatttgtc aacaagagag ggtaagcaaa gataaacttg tttaaaatag acagataaat
     6481 ctacttcctt ctcttaattt atgattcttg aaatgcgcct gctttaaggc taaactagac
     6541 gtatcaaagt taagagtttg tttttgtaac tggaaagaga tgaacaaatc aaatacataa
     6601 aagataaact catttttatt cgttttgtaa aaataaaatg catattttta aagaaagatt
     6661 gatgaaaaat gtagatttga tgtttttact tgaataaaaa gcggtttcat agtaaaataa
     6721 gaactgagaa tgatggaggt aaatcggtgg aaaatctgaa gaaaatggca ggtatcaagg
     6781 ctgctgagtt cgtgagcgat ggaatggtcg ttggacttgg aacaggctcg actgcctatt
     6841 attttgtcga agaaatcggt cgtcgtatca aggaagaagg attgcagatt acagctgtaa
     6901 caacttctag tgtgaccact aaacaggcag aaggccttaa tatcccgctc aagtctattg
     6961 accaagtaga ctttgtcgat gtgacggttg atggcgcgga tgaagtggat agtcagttta
     7021 atggaatcaa aggcggtggt ggtgcccttc tcatggaaaa ggtggtcgca acaccatcaa
     7081 aagaatacat ttgggtggtg gatgaaagca agctggtcga aaaactaggt gcttttaaat
     7141 tgccagtaga agtggttcag tatggtgcag agcaggtctt tcgtcatttt gaacgagctg
     7201 gctacaaacc aagtttccgt gaaaaagacg gtcaacgttt tgtgacggat atgcagaatt
     7261 ttatcattga cctcgccttg gatgtcattg aaaatccaat tgcttttgga caagaattgg
     7321 accatgtcgt tggtgttgtg gagcatggtt tattcaacca aatggtggat aaggtaatcg
     7381 ttgctggacg agatggagtt cagatttcaa cttcaaaaaa aggaaaatag aagggggcat
     7441 aagatgtcta aatttaatcg tattcatttg gtggtactgg attctgtagg aatcggtgca
     7501 gcaccagatg ctaataactt tgtcaatgca ggggttccag atggagcttc tgacacactg
     7561 ggacacattt caaaaacagt tggtttgaat gtcccaaaca tggctaaaat aggtcttgga
     7621 aatattcctc gtgaaactcc tcttaagact gtagcagctg aaagcaatcc aactggatat
     7681 gcaacaaaat tagaggaagt atctcttggt aaggatacta tgactggaca ctgggaaatc
     7741 atgggactca acattactga gcctttcgat actttctgga acggattccc agaagaaatc
     7801 ctgacaaaaa tcgaagaatt ctcaggacgc aaggttattc gtgaagccaa caaaccttat
     7861 tcaggaacgg ctgttatcga tgattttgga ccacgtcaga tggaaactgg agagttgatt
     7921 atctatactt cagctgaccc tgttttgcag attgctgccc acgaagacat tattcctttg
     7981 gatgaattgt accgtatctg tgaatacgct cgttcgatta cccttgagcg tcctgccctt
     8041 cttggtcgca tcattgctcg cccttatgta ggtgaaccag gtaacttcac tcgtacggca
     8101 aaccgtcgtg acttggctgt atctccattt tccccaactg ttttggataa attgaatgag
     8161 gctggtatcg atacttatgc tgtgggtaaa atcaacgata tctttaacgg tgctggtatc
     8221 aaccatgaca tgggtcacaa caagtcaaat agtcatggaa ttgatacact attgaagact
     8281 atgggacttg ctgagtttga aaaaggattc tcattcacaa acctagttga ctttgatgcc
     8341 ctttacggcc atcgtcgtaa tgctcacggt taccgtgatt gcttgcatga gtttgatgaa
     8401 cgcttacctg aaattatcgc agctatgaga gagaatgacc ttctcttgat tactgcggac
     8461 catggaaatg acccaacgta tgcaggaacg gatcacactc gggaatatat tccattgttg
     8521 gcctatagcc ctgcctttaa aggaaatggt ctcattccag taggacattt tgcagatatt
     8581 tcagcgactg ttgccgataa ctttggtgtg gaaactgcta tgattgggga aagtttctta
     8641 gataaattgg tataagatga cgcgctatgc tttgctggtg agaggtatca atgttggtgg
     8701 taagaataag gtcgtcatgg cggagcttcg tcaagaattg acaaacttgg gactggaaaa
     8761 ggttgagagc tacatcaata gtggcaatat tttctttact tcgatagatt ccaaagccca
     8821 attggttgaa aagctagaga ctttctttgc agtccattat ccatttattc agagcttttc
     8881 tttactgagt ctagaggact ttgaggcgga acttgaaaat ctaccagctt ggtggagcag
     8941 agacttggca cgaaaagatt ttctctttta cactgagggt ttggatgtgg accaagtcat
     9001 cgcgacagtt gaaagtttag agctgaaaga tgaagtgctt tattttggaa aacttgggat
     9061 tttctggggg aaattttctg aagaatccta ttctaagact gcctatcata agtacttgct
     9121 gaaggtgcct ttctaccgcc acattactat tcgtaatgct aaaacctttg acaaaattgg
     9181 tcaaatgcta aaaaaataat aaaggagaca cacaatgaca tttttaaaca aaatccatga
     9241 aactgctact ttcctgaaag aaaagggaat tgcagcccct gagttcggtc taatccttgg
     9301 atcaggactt ggagaattgg cagaagaaat cgaaaatcca gttgtagtag actatgctga
     9361 gattccaaac tggggccgtt caacagtagt cggtcatgct ggtaaattgg tatatggtga
     9421 actggcaggt cgcaaggtct tggctcttca agggcgtttc catttctatg aagggaatcc
     9481 tctggaagtg gtgactttcc cagttcgtgt gatgaaagtt cttggatgtg aaggtgttat
     9541 tgtaaccaat gcagctggcg gtatcggatt tggtcctggt accttgatgg ctatctcaga
     9601 ccatatcaac atgacggggc aaaatccatt gatgggtgaa aacttggatg actttggccc
     9661 acgtttccca gatatgtcta gggcctacac accagaatac cgtgccactg cccatgaagt
     9721 ggctaaaaaa cttaatatca agcttgatga aggtgtctat atcggagtta ctggtccgac
     9781 ttatgaaaca ccagcagaaa ttcgttccta taagacactg ggagcagatg cagttggtat
     9841 gtctacggtt cctgaagtta tcgtggcagc ccactctggc ttgaaagttc tgggaatttc
     9901 atgtatcact aactttgcgg ccggtttcca agaagaactc aatcacgaag aagttgtaga
     9961 agtgactgaa cgtgttaaag gtgatttcaa aggcttgctt aaagcgattc ttgctgaatt
    10021 gtaagaaaaa agatttaaaa gggggagtgc ctctgttttt tcaggattga ctgcctatcc
    10081 ggattaaaga agaaacagag gaatactatg agcttcttcc tgctcttata actgaaagaa
    10141 gcggaagaat aggtatgtct gatctgatag ccagcattgt gaaagacaag attctaggat
    10201 actagcatta gcttcctagc caagcagact agtatgataa ggagagatga gaatgaattg
    10261 actttctgaa tttctcagtc ttatcatata tagcacaatg agatttcgct tgagtctgct
    10321 tgtaaataaa cgaaaagaaa gataagaaat aatgaaaatt ggtcaacgaa ttatgcgctt
    10381 tggcataaaa aattaagtat cggagttgta tctgttgtag tcggctttga tttctagctc
    10441 cagctggaat ttcagccaat gaagtaaagc aagatgtaac atctgaagtg gtaataggtg
    10501 tgctagattc taaggaggaa ttgaaagagt cagaaaatga tgctccaaaa ctagaaactc
    10561 ctcttagaga ggagccaaga ctagctcctc aaacgcttcc ggaagcaagt gaagttcttg
    10621 aaaacaaaag ggaagagtca aaagtagaga taacataacc agctcaagcg gatgatatcc
    10681 gcaaggttgt tggggaatta gccaaggata taagtattac taagttgtat atgacaggtc
    10741 attctcttgg atgttaccta gctcagattg cagcggttga agcttaccaa aaatatcctg
    10801 atttttataa ccatgtattg aggaaagtga caactttcag tgctcctaaa gtgattactt
    10861 ccagaactgt ttggaatgct aagaatggtt tctgggatgt tggtttggaa agtcgtaaat
    10921 tagctgttag tggaaaaatt aagcattatg tggttgataa tgacaatgtt gtgactccct
    10981 tgattcataa taatcgtgat attgttacat ttacaggtaa ttcacgcttt aaacaccgtt
    11041 ctcgtggcta ttttgaaagt ccaatgaatg atattcctaa ctttaatatt ggtaaacaag
    11101 ctaccttgga taaacatggt tatcgtgatc cgaaattgga taaagtgcga ttctttaaga
    11161 aacaggctct gcctcgatct tctagtcaac caagcgctga accaatggaa aatattgcct
    11221 taggaaaaca ggttactcaa agttcgacag ctttcggagg agatgctaga agagctgtgg
    11281 atggcaaagt cgatggtaac tatggtcaca attctgtcac tcatacaaac ttccaatcta
    11341 agccttggtg gcaagtagat ttggctaaag aagaaaccat tcgccaaatc aatatttaca
    11401 accgaacaga cactgcccag gatagattgg caaactttga tgtcattctt ttagacagtt
    11461 ctggtaaaga aattgagtga aaacgtataa tatctcctaa agatgtgtca gcacaaatta
    11521 cgattaacca taaaaaagcg cgctatgttc ggattgagct agaaggctat aatgccctca
    11581 gtcttgcaga agttgaagtt ttccgcttta tagctacgaa tgctgaaacg gcgacacaag
    11641 tttctaagcc agttcaacca atcagtcaga ctcctgtgaa agataaaaca ttgacaattc
    11701 aacacagtgg agcttacatt gcccgctact ccataacttg ggaagaagtt ccagtagata
    11761 aagatggaaa ccaagttgtt cgtagtcatt cttgggaagg aaacggtcgc aaccagactg
    11821 cgggttttgt cctcaacctc ccaatcaaag aaaatatgag aaatctgcga gttaagattg
    11881 agaaaaagac gggcctacta tggaatagat ggcaaacaat ctatgaaaac agaccaattt
    11941 cagctcaacc ccaccgtaaa attacccatt ggggtacgac attgaattcc aaggtgagtg
    12001 acgatgatgt cttgtaatct gatggtacaa tgacagttag tttgtctagt ttataagaaa
    12061 gtactacctg agcttgaata gaactcaggt agctctctgt gaaagaaca
//
LOCUS       DAVBHS010000051        12098 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVBHS010000051 DAVBHS010000000
VERSION     DAVBHS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12098
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.051"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            51..1100
                     /locus_tag="VQ651_001027"
     CDS             51..1100
                     /locus_tag="VQ651_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET2754036.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAVHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGSNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(1200..5042)
                     /gene="spuA"
                     /locus_tag="VQ651_001028"
     CDS             complement(1200..5042)
                     /gene="spuA"
                     /locus_tag="VQ651_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HET2754037.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPGNANASLEKTEEKPAASPA
                     DPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDD
                     VEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVE
                     KLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQW
                     PDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQ
                     IFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHL
                     GNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGK
                     LGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDST
                     NKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDSKIDDAHKVAKAAFVDP
                     AKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDY
                     LKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSD
                     PKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDLFEDLEPNYYHFMDADGTPR
                     TSFGGGRLGTTHHMTKRLLIDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARAL
                     NPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPA
                     FITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKA
                     ENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHL
                     LRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQS
                     TDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKARE
                     FNLGTAFAHLRNAEVLADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTS
                     HESTAEEKPDSTPSKPEHQNEASHPEHQDPAPEARPDSTKPDTKVADAENKPSQATAD
                     SQAEQPAQEAQASSVKEAVRNESVENSSKKNIPATPDRQAELPNTGIKNENKLLFAGI
                     SLLALLGLGFLLKNKKEN"
     gene            5459..5872
                     /gene="rpsL"
                     /locus_tag="VQ651_001029"
     CDS             5459..5872
                     /gene="rpsL"
                     /locus_tag="VQ651_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET2754038.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            5892..6362
                     /gene="rpsG"
                     /locus_tag="VQ651_001030"
     CDS             5892..6362
                     /gene="rpsG"
                     /locus_tag="VQ651_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET2754039.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            6787..8868
                     /gene="fusA"
                     /locus_tag="VQ651_001031"
     CDS             6787..8868
                     /gene="fusA"
                     /locus_tag="VQ651_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET2754040.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            8972..>12098
                     /locus_tag="VQ651_001032"
     CDS             8972..>12098
                     /locus_tag="VQ651_001032"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HET2754041.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPAFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFESVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVER"
BASE COUNT         3353 a         2346 c         2839 g         3560 t
ORIGIN      
        1 ttagaataat ttttcaaatt gaaggacaga gatagacaag gagaatcaca atggtagaat
       61 tgggaatttc aacatttggg gaaataacgg agcttgaagg gactggacaa acttacagtc
      121 atgctgaacg cattcgccag ttggtggcag agattgagct ggctgacaag gttggtttgg
      181 acgtatatgg gattggcgag caccatcggg cggattttgc agtatcagcc ccagagattg
      241 tcctggccgc tggggcagtc aataccaaga aaatccgttt gaccagtgca gtcagcattc
      301 tgtcaagcat ggatccgatt cgtttgttcc aacagtatgc cactatcgat gctttgtcaa
      361 atggacgagc ggagattatg gctggaaggg gctctttcac ggaatctttc cctctgtttg
      421 gttatgactt gaaagactac gactctcttt ttgatgagaa attagacttg cttcagttag
      481 tcaatgaaaa gaccaagtta gactggcaag gtcgattgac tcaaacgatt gctggtaaag
      541 aagtttatcc tcgtccagtt caggacaaat tgcccttgtg gatagctaca ggtggtcatg
      601 tcgaatcaac agtgaagatt gctcaggctg gtctaccgat tgtatatgct attattggtg
      661 gtaatccacg ttattttaaa aagttgattc aggcttatcg tgagattgga agcgaagcgg
      721 tccatgctga caaagacttg aaagtgggag cccattcttg ggggtggatt gcggaagatg
      781 gtgagcaggc ggtgaaagat tatttccatc cgactaagca agtggtggat gctatttcca
      841 aagaccgtcc gcactggcag gaattgcgtt atgagcaata tttggagcag gtagggtcaa
      901 atggcgccat gtttgttggc aatccagatc aggtggccga aaaattgatt cgcatgatag
      961 aggatttagg attggaccgc ttcatgcttc atctaccgct tggttctatg cctcatgacc
     1021 aggttctgag agctattgaa ctcttcggaa cacaagttgc acccaaagta cgagcttatt
     1081 ttgctatgaa agaggcttaa taaaaaatcc taatcaagtt tattaggaca acaaatattg
     1141 tacgaaaatt aatcccccga gctataatgc ttgggggatt tttctatagg agggctagtt
     1201 tagttctctt ttttattttt tagtaagaaa ccgagaccaa ggagcgcaag gaggctgatt
     1261 cctgcaaata ggagtttgtt ttcgttttta attcctgtat ttggaagttc agcttgtcta
     1321 tctggggttg caggtatatt tttcttgcta gagttttcta ccgattcgtt tcgaaccgct
     1381 tcttttacag atgatgcttg tgcttcttgt gctggttgtt cagcttgtga atcagctgta
     1441 gcttggctag gtttattttc cgcatcagct actttggtat ctggtttagt agaatcaggt
     1501 ctagcttctg gagctggatc ttgatgttca gggtgagaag cttcattttg atgttcaggc
     1561 ttggaagggg ttgagtctgg tttctcttct gcagttgact catggctagt tccattttga
     1621 gagactcgaa gaacagtagc tgtaagggca ttcaatttca agcctttttc agtccattca
     1681 agtcctttcg ggttggcaat tccgactgat cctgcttggt tttcatctgc caaaacttcc
     1741 gcatttctta gatgtgcaaa ggcagttccc aaattaaatt cgcgagcttt ttcatccgca
     1801 ttgacaaaga ctgcgtagat atcgccgttt ggagcagtga tttgatagcc aatcactaca
     1861 tcctcttttt ccacaccatt ttggcctggg acagtgatga ggtggacacg gtctttgata
     1921 tcttgaagac tcttaagtcg gaaggcatct gtagattgac gaagggcaat caaacctttc
     1981 atatagtcac ggctcttgac attttcagga taagctttac catctgtagc cttagtccag
     2041 tcaaacttgt tgactgcatc actagaatcg taagagtcat ggatgaagta aggatagtca
     2101 aatgggttgc cgtccttatc acgcaacaag tgagatttgt ttggaacctt atcctctgct
     2161 actggagtct tgtaggctgg gtcacggaat tgtttagtac gtccatattc ctgaccggag
     2221 tggataaatg gagttccttg agctgtcaag accatgagat ttccaagtcg taaacgacgg
     2281 tggatttcag catagttctc agccttgctt gggtcttttt tgatagactg ggcaatgatg
     2341 tcaaagaggg tcaagttatc atgggctgcg atgtattgga tgacatctcc agggctgtca
     2401 gcttcaaagt tagttggttg agcaatgaga tttttaaaga tggtgttgac atcacgcttg
     2461 ccacctgtga taaaggcagg ttgaccttcg tttggataac cagatttgag gttgttacgg
     2521 atgtcatctg aaaagacagc gacagtatcg gtatgtttca tccaatcttg gtcagcagct
     2581 ttagtaggca tgttttcatc accggcatag gttctccaac cttcaccaag catgatgagg
     2641 tttggattga gggcgcgtgc agccttgtaa gcttcttcga tagaagcggc gtcatggtct
     2701 cccatcatat cgaaacggaa gccatccact ttgtaggtat caactaggta tttgatagag
     2761 tcaattagga gccgtttggt catatggtgg gttgtcccca agcgtccacc accaaagcta
     2821 gttcgaggtg tgccatcggc atccataaag tggtagtagt ttggttccaa atcttcaaag
     2881 agatcgactt tggctgtgtg gttataaacg acatctagga tagctcccat accacgtttg
     2941 tggatttcgt tgatgaggtt tttaaattct gcgattcgtt tttctggatt cttaggatcg
     3001 cttgagtaca taccagtcaa ggagaagtag ttttgagggt catatcccca gttgtagttg
     3061 ctgttgcttg aagcgtagtc agacaagcgt tcatggtttt tcaattcatt gacaaagtag
     3121 taagacaaga ctggaaggag ctggatatgg gttacaccca agtctttgag atagtctagt
     3181 ttttcaatga aggcttcaaa agtcccaaat ggtttggtca agtcttttgc aatggcagga
     3241 tctgaagtga aatcacgcac atgagcttcg tagataacgg cgtcttcacg agtcttgaaa
     3301 ttgtgaatct taccataagt caagtcttga ggtccgagtt tagctggatc tacaaaggcg
     3361 gctttagcca ctttatgggc atcgtcaatc ttggaatcgt cgctattcca agcagcaaga
     3421 gatttagcgt aaggatcgag tgcaagaaca gttttacctt gacgctcgat ttggtattga
     3481 taatagtagc cagtgaaatc tgtgattccg agtttgtttg tgctgtctag agtttgtttc
     3541 caagttcctc tttccccttt ttcaagagcg acagttccaa ctactttgtc agggtcattc
     3601 ttgtcgtaga caacaacaga aaccttatca gcacttggtg accaaagggt caaatcaact
     3661 tgttttcctt cttcttttag gtcagctccc agtttgccat catagctgta tgtctcatct
     3721 ttcaggcgcc agcttgtttt ggtagtgaat tggtcggaat tgtagctaac agtataagga
     3781 tgttttgtgt cagagaaatc tccgctgtag gtcactttct taccagcttc atcgattgca
     3841 acatcggtaa tagttacctt gtttcctagg tgattagtga tgttggagtg tttgaggata
     3901 tcttcttttt tagcaccgac aagtgttgaa aagctacttt caatgctaga agtgcctacg
     3961 tgttgggctc ctgtcatacg gatatcatgg acatagtatg gatttgtgta aatcgattca
     4021 tcatcgtctt ttaggaaaat ttggctatga tttttcaaat ctgtgaactt ataattttct
     4081 ttacggattt tcacgtcgtc tccttgtttg ctctcatcta gtaataaaaa tccaaattct
     4141 cttgcggctt cattaagagg aatgtcgata tagcggccat atttgcctgt agccgtaaag
     4201 tctgttccgt caggccattg agcgctactt ggatttttca catctcccca gtaccagaga
     4261 gatttcttgt catagttgcc atctgtgcgg tagtagttga cgcgaacagt tcctgcaggc
     4321 tgtggctcgt aagagaaaac cttgtaatct tggtctaacc aagcttcgtt catttttgga
     4381 actagttttt ctacagattt atcgccggtt agattttttc cagctgtatt gttgatgagg
     4441 aagctaattt tcttggcttg ttctcccttt aatttgacat ctaggtaata gccgtagtca
     4501 tctttcttgg catccttgaa ggacaaagct ccgtttggcc agttttcaga tggtttttca
     4561 acatcgtccc aagtccatag tccttgagca tccttgtttt cttcaggaag ttttttgaca
     4621 tggatacgga agtagttatc ttcgatgggc tcttctgcct tagtttcagt tgttactgga
     4681 ctagtagaag tagttggctc acttgaacga tcttgtccag tttgtggtgc tggatcggct
     4741 gggcttgcag caggtttttc ttctgttttc tctagggaag cgtttgcatt tccaggtgaa
     4801 gaaatatcac tttcgttctt accagagcca gctgctgtat cagtaagagg ttgggcaagg
     4861 gtggttgtat ttctgttgtt gggttcgtta gtggttggtg tactttcgtc ggatgagata
     4921 gttggtgtag ccatggcaag caagacaagg cttgcgccga taaggacaga accagttcca
     4981 tttttgaggg aacggatgct gtagaccatt tttttctcag tgtgagatgg tgtttttctc
     5041 ataatgattc tccaatcaat aaatttctat atcagtatag catgtagtaa aacaatatgc
     5101 aagcgttttc tttgatttgt gaaagaaatt cttacttttg agaagttttc cgtactattt
     5161 tatatatcag tataaatgac ggagataaaa atgtcacgta agccaagccg tgttattagg
     5221 aagatgatgt gttctcagct atttttattc agaaattgat ctctaacatt accaataaaa
     5281 aagaaaaata tcttgacaag caagggaaaa ttttgttaca ataatagacg gtacttttta
     5341 cttttggtct ctcaagagtg tacagggacg tgctgacaaa tgttgcaaaa gtacacacag
     5401 atgatatctg tcaccaagtg tatcatcacc aaaaataaaa aaacacagga gaatgtagat
     5461 gcctacaatt aaccaattgg ttcgcaaacc gcgtaaatca aaagtagaaa aatctaaatc
     5521 accagctttg aacgttggtt acaatagtca taaaaaagtt caaacaaacg tttcttcacc
     5581 acaaaaacgt ggtgttgcaa ctcgtgttgg aacaatgaca cctaaaaaac ctaactcagc
     5641 ccttcgtaaa ttcgctcgtg tacgtttgag caaccttatc gaagttactg cctacatccc
     5701 aggtatcgga cacaacttgc aagagcacag cgtggtgctt cttcgcggtg gacgtgtaaa
     5761 agaccttcca ggggtacgtt accatatcgt ccgtggtgca cttgatactg caggtgttaa
     5821 cgatcgtaaa caaggccgtt ctaaatacgg tactaaacgt ccaaaagcat aaggaaaggg
     5881 gataaagaga aatgagtcgt aaaaatagag ctccaaaacg tgacgtattg ccagatccgc
     5941 tatacaattc acaactagtt actcgtctta tcaaccgcgt tatgcttgat ggtaaacgtg
     6001 gtactgctgc ttcaatcgtt tacggtgctt ttgagcaaat caaagaagct actggcaacg
     6061 atgcacttga agtatttgaa acagctatgg aaaacatcat gcctgtactt gaagtacgtg
     6121 cacgtcgtgt tggtggttct aactaccaag tcccagttga agttcgtcca gaacgtcgta
     6181 caacacttgg acttcgttgg ttggtaacaa tcgctcgtct tcgtggtgaa cacacaatgc
     6241 aagaccgtct tgcaaaagaa atcttggatg ctgctaacaa cactggtgca gcagttaaga
     6301 aacgtgaaga tactcaccgt atggctgaag ctaaccgtgc attcgcacac ttccgttggt
     6361 aagataggat gcgaaagcgt taagaaagtc ccagagaaaa tagggaatcg aagcaggttg
     6421 cggttgcaac caatgagatt catctttttc tccagacttt tagcttgagc tcaactaaat
     6481 catgatgcta ggaacggtaa ggatgcaagg taaaaatagg aaactgacgc agtattcgac
     6541 gaatacaagg agttttatct ttttcacgca gcatcccgtt ccagctcaca tcggctaact
     6601 aactttagcc cgggttcaaa ttagctaaat cgattagtat tagctataac tcagcttacc
     6661 atctcgtaag ttgaaaccaa caatagcatg aaaacattga gaacgggtag gtcctgccta
     6721 tccgttttta ttaaaatcgt gttataatag aatagaaatc aaaaataaat aggagaaaca
     6781 aacctcatgg cacgcgaatt ttcacttgaa aaaactcgta atatcggtat catggctcac
     6841 gtcgatgccg gtaaaacaac aactactgag cgtattcttt actacactgg taaaatccac
     6901 aaaatcggtg aaactcacga aggtgcgtca caaatggact ggatggagca agagcaagaa
     6961 cgtggtatca cgatcacatc tgctgcgacg acagctcaat ggaacaacca ccgcgtaaac
     7021 atcatcgaca caccaggaca cgtggacttc acaatcgaag tacaacgttc tcttcgtgta
     7081 ttggatggtg cggttaccgt tcttgactca caatcaggtg ttgagcctca aactgaaaca
     7141 gtttggcgtc aagcaactga gtacggagtt ccacgtatcg tatttgccaa caaaatggac
     7201 aaaatcggtg ctgacttcct ttactctgta agcacacttc acgatcgtct tcaagcaaat
     7261 gcacacccaa tccaattgcc aatcggttct gaagatgact tccgtggtat cattgacttg
     7321 atcaagatga aagctgaaat ctatactaac gaccttggta cggatatcct tgaagaagac
     7381 atcccagctg aataccttga ccaagctcaa gaataccgtg aaaaattgat tgaagcagtt
     7441 gctgaaactg acgaagaatt gatgatgaaa tacctcgaag gtgaagaaat cactaacgaa
     7501 gaattgaaag ctggtatccg taaagcgact atcaacgttg aattcttccc agtattgtgt
     7561 ggttcagcct tcaaaaacaa aggtgttcaa ttgatgcttg atgcggttat cgactacctt
     7621 ccaagcccac ttgacatccc agcaatcaaa ggtattaacc cagatacaga cgctgaagaa
     7681 attcgtccag catctgacga agagccattt gcagctcttg ccttcaagat catgactgac
     7741 ccattcgtag gtcgtttgac attcttccgt gtttactcag gtgttcttca atcaggttca
     7801 tacgtattga atacttctaa aggtaaacgt gaacgtatcg gacgtatcct tcaaatgcac
     7861 gctaacagcc gtcaagaaat cgacactgtt tactcaggtg atatcgctgc tgccgttggt
     7921 ttgaaagata ctacaactgg tgactcattg acagatgaaa aagctaaaat catccttgag
     7981 tcaatcaacg ttccagaacc agttatccaa ttgatggttg agccaaaatc taaagctgac
     8041 caagacaaga tgggtatcgc ccttcaaaaa ttggctgaag aagatccaac attccgcgtt
     8101 gaaacaaacg ttgaaactgg tgaaacagtt atctcaggta tgggtgaact tcaccttgac
     8161 gtccttgttg atcgtatgcg tcgtgagttc aaagttgaag cgaacgtagg tgctcctcaa
     8221 gtatcttacc gtgaaacatt ccgcgcttct actcaagcac gtggattctt caaacgtcag
     8281 tctggtggta aaggtcaatt cggtgatgta tggattgaat ttactccaaa cgaagaaggt
     8341 aaaggattcg aattcgaaaa cgcaatcgtc ggtggtgtgg ttcctcgtga atttatccca
     8401 gcggttgaaa aaggtttggt agaatctatg gctaacggtg ttcttgcagg ttacccaatg
     8461 gttgacgtta aagctaagct ttatgatggt tcatatcacg atgtcgactc atctgaaact
     8521 gccttcaaga ttgcggcttc actttccctt aaagaagctg ctaaatcagc acaaccagct
     8581 atccttgaac caatgatgct tgtaacaatc actgttccag aagaaaacct tggtgatgtt
     8641 atgggtcacg taactgctcg tcgtggacgt gtagatggta tggaagcaca cggtaacagc
     8701 caaatcgttc gtgcttacgt tccacttgct gaaatgttcg gttacgcaac agttcttcgt
     8761 tctgcatctc aaggacgtgg tacattcatg atggtatttg accactacga agatgtacct
     8821 aagtcagtac aagaagaaat tattaagaaa aataaaggtg aagactaatc cgtcctcact
     8881 ctagaaggaa gtcacttagt ggcttccttt tgtctttaga aaatacctct aaatatggta
     8941 aaatagtaga agaataatgt gaggaaaatg aatgtcaaat agttttgaaa ttttgatgaa
     9001 tcaattgggg atgcctgctg aaatgagaca ggctcctgct ttagcacagg ccaatattga
     9061 gcgagttgtg gttcataaaa ttagtaaggt atgggagttt catttcgtat tttctaatat
     9121 tttaccgatt gaaatctttt tagaattaaa gaaaggtttg agcgaagaat tttctaagac
     9181 aggcaataaa gctgtttttg aaattaaggc tcggtctcaa gaattttcaa atcagctctt
     9241 gcagtcctac tatagggagg ctttctctga aggtccatgt gctagtcaag gttttaagtc
     9301 cctttatcaa aatttgcaag ttcgtgctga gggtaatcag ctatttattg aaggatctga
     9361 agcgattgat aaggaacatt ttaagaagaa tcatcttcct aatttagcca aacaacttga
     9421 aaagtttggt tttccaactt ttaactgtca agtcgagaag aatgatgtcc tgacccaaga
     9481 gcaggaagag gcctttcatg ctgaaaatga gcagattgtt caagctgcca atgaggaagc
     9541 gctccgtgct atggaacaac tggagcagat ggcacctcct ccagcggaag agaaaccagc
     9601 ctttgatttt caagcgaaaa aagctgcagc taaacccaag ctggataagg cggagattac
     9661 tcctatgatc gaagtgacga cagaggaaaa tcgtctggta tttgaagggg ttgtttttga
     9721 tgtggagcaa aaagtgacta gaacaggtcg tgttttaatc aactttaaaa tgacggacta
     9781 tacttcaagt ttttctatgc aaaagtgggt taaaaacgag gaagaggccc agaagtttga
     9841 cctcatcaag aagaattctt ggctccgagt tcgagggaat gtggagatga ataacttcac
     9901 acgcgatttg actatgaacg tacaggatct gcaggaagtt gttcactatg agcggaagga
     9961 tttgatgcca gaaggtgagc gtcgggttga gtttcatgct catactaaca tgtcgactat
    10021 ggatgctttg ccagaggtcg aagagattgt tgcaacagct gctaagtggg gacacaaggc
    10081 ggttgctatc acggaccatg ggaatgtcca gtcctttcca catggctata aggcggctaa
    10141 gaaagcggga atccagctga tctatgggat ggaagccaat atcgtggagg accgtgtccc
    10201 tatcgtctat aacgaagtgg agatggactt gtcagaagca acctacgtgg tctttgacgt
    10261 ggaaacgacg ggactttcag ctatctataa tgacttgatt caggttgcgg cttctaagat
    10321 gtacaagggg aatgttattg ctgaatttga tgaatttatc aatcctgggc atcccttgtc
    10381 agcctttact acagagttaa ctggaattac agatgatcat gtcaaaaatg ccaaaccact
    10441 agaacaagtt ttgcaagaat tccaagaatt ttgcaaggat acggtcctag ttgcccacaa
    10501 tgctaccttt gacgttggct ttatgaatgc taattatgag cggcatgatc ttccaaagat
    10561 tagtcagcca gttattgata cgctggagtt tgctagaaac ctctatcctg agtataaacg
    10621 ccatggtttg gggcctttga ccaagcgttt tggtgtggcc ttggaacatc accacatggc
    10681 caactacgat gcggaagcga ctggtcgtct gcttttcatc tttatcaaag aggtagcaga
    10741 aaaacatggt gtgaccgatt tagctagact caacattgat ctaatcagtc cagattctta
    10801 caaaaaagct cggatcaagc atgcgaccat ctatgtcaag aatcaggtag gtctaaaaaa
    10861 tatctttaag ctggtttcct tgtctaatac caagtatttt gaaggagtgc cacggattcc
    10921 gagaacggtt ctagatgccc atcgagaggg cttgatttta ggttcagcct gttcagaggg
    10981 tgaagttttt gacgtggtcg tttctcaagg tgtggatgcg gcggttgagg tggccaagta
    11041 ttatgatttt atcgaggtca tgccaccggc tatctatgca cccttgattg ccaaagagca
    11101 ggtcaaggat atggaggaac tccagaccat tatcaagagt ttgatagagg ttggagaccg
    11161 ccttggcaag cctgttctgg ctacgggaaa tgttcactat atcgaaccgg aagaagagat
    11221 ttatcgtgaa attatcgtcc gtagtttggg acagggtgcg atgattaatc gaactatcgg
    11281 tcatggtgaa catgcccaac cagcaccact tccaaaggct cattttcgaa cgactaatga
    11341 gatgttggat gaatttgcct ttttgggaga ggaactggct cgtaaactgg ttattgaaaa
    11401 caccaatgcc ttggcagaaa tatttgaatc cgttgaagtc gttaagggtg acttgtatac
    11461 gcctttcatc gacaaggctg aagaaacagt tgctgagttg acctataaga aagcttttga
    11521 gatttatgga aatccgctgc cagatattgt tgatttgcgg attgaaaaag aattaacatc
    11581 catactgggg aatggatttg ctgtgattta tctggcatcg cagatgctgg tgcaacgttc
    11641 taatgaacgg ggttatttgg ttggttctcg tgggtctgtc ggatctagtt tcgttgcgac
    11701 catgattggg attacggagg tcaatcctct ctctcctcac tatgtctgtg gtcagtgtca
    11761 gtacagtgag tttatcacag atggttcgta cggttcagga tttgatatgc cccataagga
    11821 ctgtccaaac tgtggtcaca aactcagtaa aaacggacag gatattccgt ttgagacctt
    11881 ccttggtttt gatggggata aggttcctga tattgacttg aacttctcgg gagaagatca
    11941 gcctagcgcc cacttggatg tgcgtgatat ctttggtgaa gaatatgcct tccgtgcggg
    12001 aacggttggt acggtagctg ccaagactgc ctatggattt gtcaagggtt acgagcgaga
    12061 ttatggcaag ttttatcgtg atgcagaagt agaacgcc
//
LOCUS       DAVBHS010000052        12032 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVBHS010000052 DAVBHS010000000
VERSION     DAVBHS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12032
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.052"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..907
                     /gene="lytB"
                     /locus_tag="VQ651_001033"
     CDS             <1..907
                     /gene="lytB"
                     /locus_tag="VQ651_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HET2754042.1"
                     /translation="YMAKNETVDGYQLGSDGKWLGGKATNENAAYYQVVPVTANVYDS
                     DGEKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYES
                     DGHRFYHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNY
                     SAEELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKD
                     KNNFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEY
                     ASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(968..2623)
                     /gene="pavA"
                     /locus_tag="VQ651_001034"
     CDS             complement(968..2623)
                     /gene="pavA"
                     /locus_tag="VQ651_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HET2754043.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPLANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            2728..3225
                     /gene="ybeY"
                     /locus_tag="VQ651_001035"
     CDS             2728..3225
                     /gene="ybeY"
                     /locus_tag="VQ651_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET2754044.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLGYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            3206..3601
                     /locus_tag="VQ651_001036"
     CDS             3206..3601
                     /locus_tag="VQ651_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET2754045.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            3618..4517
                     /gene="era"
                     /locus_tag="VQ651_001037"
     CDS             3618..4517
                     /gene="era"
                     /locus_tag="VQ651_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET2754046.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPDRFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            4566..5390
                     /gene="mutM"
                     /locus_tag="VQ651_001038"
     CDS             4566..5390
                     /gene="mutM"
                     /locus_tag="VQ651_001038"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HET2754047.1"
                     /translation="MPELPEVETVCRCLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQGCVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            5390..5995
                     /locus_tag="VQ651_001039"
     CDS             5390..5995
                     /locus_tag="VQ651_001039"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET2754048.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFWQEIILENGELNRPLLASLIFSNPDEREWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            5982..7181
                     /locus_tag="VQ651_001040"
     CDS             5982..7181
                     /locus_tag="VQ651_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HET2754049.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            7181..7330
                     /gene="rpmG"
                     /locus_tag="VQ651_001041"
     CDS             7181..7330
                     /gene="rpmG"
                     /locus_tag="VQ651_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2754050.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            7370..7603
                     /gene="secG"
                     /locus_tag="VQ651_001042"
     CDS             7370..7603
                     /gene="secG"
                     /locus_tag="VQ651_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET2754051.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            7705..10059
                     /gene="rnr"
                     /locus_tag="VQ651_001043"
     CDS             7705..10059
                     /gene="rnr"
                     /locus_tag="VQ651_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HET2754052.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSHSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            10022..10489
                     /gene="smpB"
                     /locus_tag="VQ651_001044"
     CDS             10022..10489
                     /gene="smpB"
                     /locus_tag="VQ651_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET2754053.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            10505..11365
                     /gene="tehB"
                     /locus_tag="VQ651_001045"
     CDS             10505..11365
                     /gene="tehB"
                     /locus_tag="VQ651_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HET2754054.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEDVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            11438..>12032
                     /gene="coiA"
                     /locus_tag="VQ651_001046"
     CDS             11438..>12032
                     /gene="coiA"
                     /locus_tag="VQ651_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HET2754055.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQG"
BASE COUNT         3496 a         2226 c         2800 g         3510 t
ORIGIN      
        1 ttacatggcg aaaaatgaga cagtagatgg ttatcagctt ggaagcgatg gtaaatggct
       61 tggaggaaaa gctacaaatg aaaatgctgc ttactatcaa gtagtgcctg ttacagccaa
      121 tgtttatgat tcagatggtg aaaagctttc ctatatatcg caaggtagtg tcgtatggct
      181 agataaggat agaaaaagtg atgacaagcg cttggctatt actatttctg gtttgtcagg
      241 ctatatgaaa acagaagatt tacaagcgct agatgctagt aaggacttta tcccttatta
      301 tgagagtgat ggccaccgtt tttatcacta tgtggctcag aatgctagta tcccagtagc
      361 ttctcatctt tctgatatgg aagtaggcaa gaaatattat tcggcagatg gcctgcattt
      421 tgatggtttt aagcttgaga atcccttcct tttcaaagat ttaacagagg ctacaaacta
      481 cagtgctgaa gaattggata aggtatttag tttgctaaac attaacaata gccttttgga
      541 gaacaagggc gctactttta aggaagccga agaacattac catatcaatg ctctttatct
      601 ccttgcccat agtgccctag aaagtaactg gggaagaagt aaaattgcca aagataagaa
      661 taatttcttt ggcattacag cctatgatac gaccccttac ctttctgcta agacatttga
      721 tgatgtggat aagggaattt taggtgcaac caagtggatt aaggaaaatt atatcgatag
      781 gggaagaact ttccttggaa acaaggcttc tggtatgaat gtggaatatg cttcagaccc
      841 ttattggggc gaaaaaattg ctagtgtgat gatgaaaatc aatgagaagc taggtggcaa
      901 agattagtac tataagtgaa tatgatttga gtgaatagta agttaaaaat cctgatttca
      961 agtaaaatca ggattttttc atggatgcaa tttttttgga gtctggtgtg acgcggaggg
     1021 tcttttgtcc tgtgtaagtg acaaagccgg gttttccacc agttggttta ttgagttttt
     1081 tgacttcaat catatctacc tgcaccagat tcgacaggcg cccttgagag aagtaggcag
     1141 ctaactctgc tgcgtctgtc ttgactgcat cagatgggtc aagatttcct gagatgacaa
     1201 catggcttcc aggaatgtcc ttagcatgga accaaagttc ctccttgcgg gccattttaa
     1261 aggtcaattc ctcattttga agattgtttc gtccgacata gatgatggtt ttgccatcgc
     1321 ttgctagata ttgttctagt tttttgcgtt tctggatttt ctcccgttgt cttctgcgga
     1381 taaaacctgt ttgaatcaat tcttcacgga tttcagcgat ttcttccagt ccagcttggt
     1441 tgaggacggt ttctacactt tccagataga gaatagtggc tttggtttct tcaatcaaat
     1501 cagtcaagta tttgacagct tctttgagtt tctgataccg tttaaaatag cgttgggcat
     1561 tctggttggg agtcagagcc ttatcaagcg caatcatgat aggttggttg gtatagtagt
     1621 tgtctaggat aacctggtct tggtcgttag gcacttggtg gaggaaggtt gtcagcaatt
     1681 ctcctttttg acgaaattct tcagcgttgt ctgtcgccag taactctttt tcctgttttt
     1741 tgagtttgtg tcggtttttc tgaagttcat tttcaacacg acgaatcagt tcactggcct
     1801 gctgtttgac gcggtcgcgc tcagccttat ccttatagta ggtgtccaac aaatcagaaa
     1861 gatttgcaag aggctctccc acctgatttg caaaaggaac tggactgaag gaagtctcag
     1921 tcaagcatgg cttggtttct tgattgaaaa aatttcggaa agcggaaagt ttttcactaa
     1981 ccagtatcct ttccaattca tttgccgtat cgcgtcccag accttgaaag aggctttgaa
     2041 gattttttgc tgttagttct tgggtttgca ggatttcaaa gagcttttca tccttgatag
     2101 taaaaggatt gagagatttt gtacttggcg gagcgatata ggtcgatcct ggaagtaagg
     2161 tgcggtagct attttgtgaa aagccgacgt gtttgataac ttcgaggatt ttatgactgc
     2221 ttttatcgac cagtagaata ttactgtgtt tccccataat ttcgataatc aaggtagcct
     2281 ggatatggtc tccaatctcg tttttattgg aaactgtaat ttccacaata cggtcatttt
     2341 ccacttgctc aatcgactca atcagggccc cctgcaaata ctttctcaaa accatgataa
     2401 aggtagaagg ttgggctgga ttttcaaaag tcgtttgggt cagctgaatg cgtccaaaaa
     2461 ctggatgggc agaaaggagc aggcgatggc tttggcgatt gctgcggatt tgcaagacca
     2521 actcttgttc aaaaggctga ttgattttct ggatgcgacc attcactaat tcgcttcgca
     2581 attcctcaac tatgtggtgt aaaaaaaatc cgtcaaatga catcgttctc tccttgtgat
     2641 tgtattccat agtattatat caaaaaggta gaataaaatc atggaaatgt ggtataataa
     2701 agccaagtaa agagaaacga gaagcacatg tatattgaaa tggtagatga aactggtcaa
     2761 gtttcaaaag aaatgttgca acaaacccaa gaaattttgg aatttgcagc ccaaaaatta
     2821 ggaaaagaag acaaggagat ggcagtcact tttgtgacca atgagcgtag tcatgaactt
     2881 aatctggggt accgtgacac cgaccgtccg acagatgtca tcagccttga gtataaacca
     2941 gaattggaaa ttgcctttga cgaagaggat ttgcttgaaa atccagaatt ggcagagatg
     3001 atgtctgagt ttgatgccta tattggggaa ttgttcatct ctatcgataa ggctcatgag
     3061 caggccgaag aatatggtca cagctttgag cgtgagatgg gcttcttggc agtacacggc
     3121 tttttacata ttaacggcta tgatcactat actccggaag aagaagcgga gatgttcggt
     3181 ttacaagaag aaattttgac agcctatgga ctcacaagac aataaacgaa aatggaaaaa
     3241 tcgtgacctg atatccagtt tagaatttgc catcacaggg atttttactg ctatcaagga
     3301 agaacgcaat atgcgaaaac atgcagtgac ggctctagtg gtcatccttg caggttttgg
     3361 ttttcaggtg tcacgaatcg aatggctctt tctcctattg agcattttct tggtggtagc
     3421 ctttgaaatt atcaattctg ctatcgaaaa tgtggtggat ttggccagtc actatcactt
     3481 ttccatgctg gctaaaaatg ccaaggatat ggcggctggc gcggtattag tggtttctct
     3541 ttttgcagcc ttaacaggcg cattgatttt tctcccacga atctgggatt tattatttta
     3601 aacagtaaga ggaaattatg acttttaaat caggctttgt agccatttta ggacgtccca
     3661 atgttgggaa gtcaaccttt ttaaatcacg ttatggggca aaagattgcc atcatgagtg
     3721 acaaggcgca gacaacgcgc aataaaatca tgggaattta cacgactgat aaggagcaaa
     3781 ttgtctttat cgacacacca gggattcaca aacctaaaac agctctcgga gatttcatgg
     3841 ttgagtctgc ctacagtacc cttcgcgaag tggacactgt tcttttcatg gtgcctgctg
     3901 atgaagcgcg tggtaagggg gacgatatga ttatcgagcg tctcaaggct gccaaggttc
     3961 ctgtgatttt ggtggtgaat aaaatcgata aggtccatcc agaccagctc ttgtctcaga
     4021 ttgatgactt ccgtaatcaa atggacttta aggaaattgt tccaatctca gcccttcagg
     4081 gaaataacgt gtctcgtcta gtggatattt tgagtgaaaa tctggatgaa ggtttccaat
     4141 atttcccgtc tgatcaaatc acagaccatc cagaccgttt cttagtttca gaaatggttc
     4201 gcgagaaagt cttgcaccta actcgtgaag agattccgca ttctgtagca gtagttgttg
     4261 actctatgaa acgagacgaa gagacagaca aggttcacat ccgtgcaacc atcatggtcg
     4321 agcgcgatag ccaaaaaggg attatcatcg gtaaaggtgg cgctatgctt aagaaaatcg
     4381 gtagcatggc ccgtcgtgat atcgaactca tgctaggaga caaggtcttc ctagaaacct
     4441 gggtcaaggt caagaaaaac tggcgcgata aaaagctaga tttggctgac tttggctata
     4501 atgaaagaga atactaagta gaggtgggct catgcctgct tcttgttttt acagaaggag
     4561 gacttatgcc tgaattacct gaggttgaaa ccgtttgtcg ttgcttagaa aaattgatta
     4621 taggaaagaa gatttcgagt atagaaattc gctaccccaa gatgattaag acggatttgg
     4681 aagagtttca aagggaattg cctagtcaga ttatcgagtc aatgggacgt cgtggaaaat
     4741 atttgctttt ttatctgaca gacaaggttt tgatttccca tttgcggatg gagggcaagt
     4801 atttttacta tccagaccaa ggacctgaac gcaagcatgc ccatgttttc tttcattttg
     4861 aagatggtgg cacgcttgtt tatgaggatg ttcgcaagtt tggaaccatg gaactcttgg
     4921 tgcctgacct tttagacgcc tactttattt ctaaaaaatt aggtcctgaa ccaagcgaac
     4981 aagactttga tttacaggtc tttcaatctg cccttgccaa gtccaaaaag cctatcaaat
     5041 cccatctcct agaccagacc ttggtagctg gacttggcaa tatctatgtg gatgaggttc
     5101 tctggcgagc tcaggttcat ccagctagat cttcccagac tttgacagca gaagaagcga
     5161 cagctattca tgaccaaacc attgctgttt tgagccaggc tgttgaaaaa ggcggttcca
     5221 ccattcggac ctataccaat gcctttgggg aagatggaag catgcaggat tttcatcagg
     5281 tctatgataa gactggtcaa ggatgtgtac gctgtggtac catcattgag aaaattcaac
     5341 taggtggacg tggaacccac ttttgtccaa actgtcaaag gagggactga tgggaaaaat
     5401 catcggaatc actgggggaa ttgcctctgg taagtcaact gtgacaaatt ttctaagaca
     5461 gcaaggcttt caagtagtgg atgccgacgc agtcgtccac caactacaga aacctggtgg
     5521 tcgtctgttt gaggctctag tacagcactt ttggcaagaa atcattcttg aaaacggaga
     5581 actcaatcgc cctctcctag ctagtctcat cttttcaaat cctgatgaac gagaatggtc
     5641 taagcaaatt caaggggaga ttatccgtga ggaactggct actttgagag aacagttggc
     5701 tcagacagaa gagattttct tcatggatat tcccctactt tttgagcagg actacagcga
     5761 ttggtttgct gagacttggt tggtctatgt ggaccgagat gcccaagtag aacgcttaat
     5821 gaaaagggac cagttgtcca aagatgaagc tgagtctcgt ctggcagccc agtggccttt
     5881 agaaaaaaag aaagatttgg ccagccaggt tcttgataat aatggcaatc agaaccagct
     5941 tcttaatcaa gtgcatatcc ttcttgaggg aggtaggcaa gatgacagag attaactgga
     6001 aggataatct gcgcattgcc tggtttggta attttctgac aggagccagt atttctttgg
     6061 ttgtaccttt tatgcctatc ttcgtggaaa atctaggtgt agggagtgag caagtcgctt
     6121 tttatgcagg cttagcaatt tctgtctctg ctatttccgc ggcgctcttt tctcctattt
     6181 ggggtattct tgctgacaaa tacggccgaa aacccatgat gattcgggca ggtcttgcta
     6241 tgactatcac tatgggaggc ttggcctttg tcccaaatat ctattggtta atctttcttc
     6301 gtttactaaa cggtgtattt gcaggttttg ttcctaatgc aacggcactg atagccagtc
     6361 aggttccaaa ggagaaatca ggctctgcct taggtacttt gtctacaggc gtagttgcag
     6421 gtactctaac tggtcccttt attggtggct ttatcgcaga attatttggc attcgtacag
     6481 ttttcttact ggttggtagt tttctatttt tagctgctat tttgactatt tgctttatca
     6541 aggaagattt tcaaccagta gccaaggaaa aggctattcc aacaaaggaa ttatttacct
     6601 cggttaaata tccctatctt ttgctcaatc tctttttaac cagttttgtc atccaatttt
     6661 cagctcaatc gattggccct attttggctc tttatgtacg cgatttaggg cagacagaga
     6721 atcttctttt tgtctctggt ttgattgtgt ccagtatggg cttttccagc atgatgagtg
     6781 caggagtcat gggcaagcta ggtgacaagg tgggcaatca tcgtctcttg gttgtcgccc
     6841 agttttattc agtcatcatc tatctcctct gtgccaatgc ctctagcccc cttcaactag
     6901 gactctatcg tttcctcttt ggattgggaa ccggtgcctt gattcccggg gttaatgccc
     6961 tactcagcaa aatgactccc aaagccggca tttcgagggt ctttgccttc aatcaggtat
     7021 tcttttatct gggaggtgtt gttggtccca tggcaggttc tgcagtagca ggtcaatttg
     7081 gctaccatgc tgtcttttat gcgacgagcc tttgtgttgc ctttagttgt ctctttaacc
     7141 tgattcaatt tcgaacatta ttaaaagtaa aggaaatcta gtgcgagtaa aaattaatct
     7201 caaatgctcc tcttgtggca gtatcaatta cctaaccagt aaaaattcaa aaacccatcc
     7261 agacaagatt gaggttttaa agtattgtcc caaggaaaga aaagtaaccc tacatcttga
     7321 atctaagtag attttatggt aaaataatag ggattttaag gagtttgata tgtataacct
     7381 attattaacc attttattag tattatctgt tgtgattgtg attgcaattt tcatgcaacc
     7441 aaccaaaaac caatccagca atgtatttga tgccagttca ggtgatttgt ttgaacgtag
     7501 taaagctcgc ggttttgaag ctgtaatgca gcgtttgaca gggattttag tctttttctg
     7561 gctagccatt gccttagcat tgacggtatt atcaagtaga taagaaaata atgggcagga
     7621 ctaggtcttt gcctcttttt atttttaaag gatgtttgag aaggttttac agtaaaagaa
     7681 aattaaaaaa tctagaaaga aaatatgaaa gatagaataa aagaatattt acaagacaag
     7741 ggaaaggtga ctgttaatga tttggctcag gctttgggaa aagacagttc caaggatttt
     7801 cgtgagttga ttaaaacctt gtccttaatg gaaagaaagc accaaattcg ttttgaagaa
     7861 gatggtagtc tgacattaga aattaagaaa aaacatgaga ttaccctcaa ggggattttt
     7921 catgcccata aaaatggctt tggctttgtt agtctggaag gcgaggagga cgaccttttt
     7981 gtagggaaaa atgatgtcaa ctatgctatt gatggtgata ccgtcgaggt agtgattaag
     8041 aaagtcgctg accgcaataa gggaacagca gcagaagcca aaattattga tatcctagaa
     8101 cacagtttga caacagttgt cgggcaaatc gttctggatc aggaaaaacc taagtatgct
     8161 ggctatattc gttcaaaaaa tcagaaaatc agtcaaccga tttatgttaa gaaaccagcc
     8221 ctaaaattag aaggaacaga agttctcaaa gtctttatcg ataaataccc aagcaagaaa
     8281 catgatttct ttgtcgcgag tgttctcgat gtagtgggac actcaacgga tgtcggaatt
     8341 gatgttcttg aggtcttgga atcaatggac attgtatccg agtttccaga agctgttgtt
     8401 aaggaagcag aaagtgtgcc tgatgctccg tctcaaaagg atatggaagg tcgtctggat
     8461 ctaagagatg aaattacctt taccattgac ggtgcggatg ccaaggactt ggacgatgca
     8521 gtgcatatca aggctctgaa aaatggcaat ctggagcttg gggttcacat cgcagatgtt
     8581 tcttattatg tgaccgaggg gtctgccctt gacaaggaag cccttaaccg tgcgacttct
     8641 gtttacgtga cagaccgagt ggtgccaatg cttccagaac gactatcaaa tggcatctgc
     8701 tctctcaatc cccaagttga ccgcctgacc cagtctgcta ttatggagat tgataaacat
     8761 ggtcgtgtgg tcaactatac cattacacaa acagttatca agaccagttt tcgtatgacc
     8821 tatagcgatg tcaatgatat cctagctggc gatgaagaaa agagaaaaga atatcataaa
     8881 attgtatcaa gtatcgaact catggccaag cttcatgaaa ctttagaaaa catgcgtgtg
     8941 aaacgtggag ctctcaattt tgataccaat gaagcgaaga ttttagtgga taaacaaggt
     9001 aagcctgttg atatcgttct tcggcagcgt ggtattgccg agcggatgat tgagtctttt
     9061 atgttgatgg ctaatgaaac agttgccgaa catttcagca agttggattt gccttttatc
     9121 tatcgaattc acgaggagcc taaggctgaa aaggttcaga agtttattga ttatgcttcg
     9181 agttttggct tgcgcattta tggaactgcc agtgagatta gtcaggaggc acttcaagac
     9241 atcatgcgtg ctgttgaggg agaaccttat gcagatgtat tgtccatgat gcttcttcgc
     9301 tctatgcagc aggctcgtta ttcggagcac aatcacggcc actatggact agctgctgac
     9361 tattatactc actttaccag tccaattcgt cgttatccag accttcttgt tcaccgtatg
     9421 attcgggatt acggccgttc taaggaaata gcagagcatt ttgaacaagt gattccagag
     9481 attgcgaccc agtcttccaa ccgtgaacgt cgtgccatag aagctgagcg tgaagtcgaa
     9541 gccatgaaaa aggctgagta tatggaagaa tacgtgggtg aagagtatga tgcagttgta
     9601 tcaagtattg tcaaattcgg tctctttgtc gaattgccaa acacagttga aggcttgatt
     9661 cacatcacta atctacctga attttatcat ttcaatgagc gtgatttgac tcttcgtgga
     9721 gaaaaatcag gtatcacttt ccgagtgggt cagcagatcc gtatccgtgt tgaaagagcg
     9781 gataaaatga ctggagagat tgatttttca ttcgtaccta gtgagtttga tgtgattgaa
     9841 aaaggcttga aacagtctag tcatagtggc agagggcgtg gttcaaatcg tcgttcggat
     9901 aagaaggaag acaagagaaa atcaggacgc tcaaatgata agcgtaagca ttcacaaaaa
     9961 gacaagaaga aaaaaggaaa gaaacctttt tacaaggaag tagctaagaa aggagccaag
    10021 catggcaaag ggcgagggaa aggtcgtcgc acaaaataaa aaggcacgcc acgactatac
    10081 aatcgtagat acgctagagg cagggatggt cctgactgga actgaaatca agagtgtacg
    10141 agctgctcga attaatctca aggatggctt tgctcaagtg aaaaatggag aagtttggct
    10201 gagcaatgtt catatcgcgc cttacgaaga gggcaatatc tggaaccagg aaccagaacg
    10261 tcgtcgtaaa ctcctgctcc ataaaaagca aattcaaaaa ttggaacaag agaccaaagg
    10321 gacaggaatg accttagttc cccttaaggt ctatataaaa gatggctacg ctaagcttct
    10381 tttaggactt gccaaaggga agcatgacta tgacaaacgg gagtctatca aacgtcgtga
    10441 gcaaaatcga gatatcgcgc gtgtgatgaa agctgttaat cagcgataaa aagaggaatt
    10501 gaaaatggaa aaattagttg cctataaacg catgcctttg tggaataaac aaacaatgcc
    10561 tgaagatgtt cagcaaaagc acaatacaaa agttgggact tgggggaaaa ttactgtctt
    10621 gaagggagct ctcaagttta ttgaattgac agaagaaggg gaagttctag ctgaacacct
    10681 ctttgaagca ggggcagaca atccaatggc ccaacctcaa gcctggcacc gagtggaagc
    10741 tgccacagat gatgtggaat ggtacttgga attttattgt aaacctgagg attattttgc
    10801 taaaaaatac aataccaatc ctgttcattc agaggtccta gaggccatgc agacagtgaa
    10861 acaagggaaa gctttggatt tgggttgtgg tcaggggcgt aattctcttt ttctagccca
    10921 gcaagatttt gatgtgacgg ctgtagatca aaatggacta gctcttgaaa tcttgcaaag
    10981 cattgtggag caggaagatt tggacatgcc tgttggcctt tacgatatca attcagctag
    11041 cattgaacaa gaatatgatt ttatcgtttc aacagttgtt ctcatgtttc tacaagcgga
    11101 ccgcattcca gctattattc aaaatatgca ggagaaaacc agtgttggtg gttacaacct
    11161 tatcgtttgt gccatggaca cggaggatta tccttgctcg gttaatttcc cattcacctt
    11221 taaagaagga gaactggcag actattacaa ggattgggaa ttggttaagt acaatgaaaa
    11281 tccaggccat ttgcaccgtc gcgatgagaa tggcaatcgt attcaactac gctttgcgac
    11341 cttactagct aagaaaatca agtaaacaca catgaagatt aggaattttc ctgatctttt
    11401 ttctttttta cgaatgatat agaaaaggag ggaattcatg tttgttgcga gagatgctag
    11461 gggagaattg gtaaatgtgt tagaggataa acttgagaag caagcataca cctgcccagc
    11521 ttgtggaggc cagctccatt tgcgtcaagg accaagtgta cggacgcatt ttgcccataa
    11581 atccttaaaa gactgtgatt ttttctttga aaatgaaagt ccagaacacc tggccaataa
    11641 ggaatccctc tatcactggt tgaaaaaaga gacaaaggtt caattagagt acccgctttc
    11701 agaacttaaa cagattgcgg atgtatttgt aaatggcaat ctagctctag aagttcagtg
    11761 tagtcccttg cctcagaaag tccttaaaga gcgaagtgag ggatatcgta gtcagggtta
    11821 ccaagtactg tggttgctgg gtcaaaaact gtggctcaag gagcgtttga ctcgtctaca
    11881 gcaaggtttt ctttatttca gtcaaaacat gggcttttat gtttgggaat tagacaagga
    11941 aaaacaagtt ttaagactca aatacctgat ttaccaggat ctccgcggta aactccatta
    12001 tcaaatcaag gaattttcct atggtcaagg ta
//
LOCUS       DAVBHS010000053        11954 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVBHS010000053 DAVBHS010000000
VERSION     DAVBHS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11954
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.053"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(101..1732)
                     /locus_tag="VQ651_001047"
     CDS             complement(101..1732)
                     /locus_tag="VQ651_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HET2754056.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGITITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2025..7004
                     /locus_tag="VQ651_001048"
     CDS             2025..7004
                     /locus_tag="VQ651_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HET2754057.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLRYQGHEVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNKVLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAVLASLGLASLALTLRRKREDKD"
     gene            7094..8290
                     /locus_tag="VQ651_001049"
     CDS             7094..8290
                     /locus_tag="VQ651_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET2754058.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8426..8953
                     /locus_tag="VQ651_001050"
     CDS             8426..8953
                     /locus_tag="VQ651_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET2754059.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9030..9386
                     /gene="glnR"
                     /locus_tag="VQ651_001051"
     CDS             9030..9386
                     /gene="glnR"
                     /locus_tag="VQ651_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HET2754060.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9423..10769
                     /gene="glnA"
                     /locus_tag="VQ651_001052"
     CDS             9423..10769
                     /gene="glnA"
                     /locus_tag="VQ651_001052"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET2754061.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10865..10984)
                     /locus_tag="VQ651_001053"
     CDS             complement(10865..10984)
                     /locus_tag="VQ651_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET2754062.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11472..11954
                     /locus_tag="VQ651_001054"
                     /pseudo
     CDS             11472..11954
                     /locus_tag="VQ651_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3840 a         2180 c         2568 g         3366 t
ORIGIN      
        1 tactatatat ctgcagaata ctctttcagt ttcattcagc ctagtcttgc ttaggaaaaa
       61 gtaaaaagcc atccaattat gatggatagc ttcttggttc ttagatttgt tctgcaaaaa
      121 ccttttcagc aaggttcata gcgtggtctg atacacgagt gtagtgtgag atgatgtcga
      181 taaagttgac cccagcttgt gttgaacatt cgcctttgtt gaggcgtttg atgtgggttt
      241 ttctgagaac acgttctatc ttattgattg cttcatgacg ttctacaaga ctgcgtgctt
      301 tttcaatatc attgttttcc acactatcca gagcatcttt gataaagtca ctagtttggc
      361 ggtaaacttc ctctaattct ttcaaggcgg catcagaaaa ttcaacattt ttccgttgaa
      421 gatagtcagt cagattgagt agagcctccg tgtggtctcc aatccgttcc aaatcacggg
      481 aggaatcaag gatattggta agcacttcac tttctttttg gctgagagct tcgcttgaaa
      541 gggcaatgag ataacgtgtt aattgctcat cgatggtgtt aattgcttct tcggttttat
      601 gccctttttc agcaactttt tcatccaagt caatgatgta cttataggaa aggtcaaagg
      661 ctttagcagc gtagtttcct aagtgcaaga gctctttctt agcatttcct agagcgatag
      721 atggggcctg tttgatgaaa tgttcatcaa gatataaggg ttcgtatttg acaacctcgt
      781 cctctccagg aataatcttg gttacaaagt aagccagagc tccgataaat ggaaattgga
      841 caatggtgtt ggtaatatta aaggttccgt gagcaaaggc gatggtcatt tccggtgcta
      901 gatttagcgt agcttcaaac caatgaatca ggacagtaaa aggaactaga aaaataacgc
      961 agacgactgt tccgataacg ttgaaggcaa catgagctcc tgctacccgt ttagctgcaa
     1021 tattagcccc taaagaggca atgatggctg taatggttat cccgatattg tcaccaaata
     1081 gaactggcaa agctccctgt aggtcaatta gattgccggc gtagaggttt tgtaaaatcc
     1141 caatggtagc cgaagaagct tgaattagca aggtcaagcc ggtaccgaca aagacaccca
     1201 aaacaggatt cttacttagc tcaatcatat agtccttaaa gacctgtaaa tccttgagtg
     1261 gagccattgc gccgctcatg agattgaggg caaaaaagat accaccgaca ccaaagagga
     1321 tgcgtccgat attattgact gtccgatttt ttgtaaaaaa gagacagacg gcaccgataa
     1381 agagcatagg tagggcatag ttacctaatt taaaaccgat gagaaaggat gtgacagttg
     1441 tcccaatatt agcacccatg acaatcccga tagcctgacg taaggttaag agaccggcac
     1501 tgaccaggcc gactgtgata actgttacac cagaactaga ctgaattaga gcagtcatcc
     1561 caataccaac cagaactcca aagaaaggat tactagtata tttgtcaatg taaaaacgaa
     1621 ggcgatctcc agcagcttgt tgcaaaccgt ctcccatggt cttgatgcta tataagaata
     1681 gccccagacc acctaaaaag tgaaataaaa tttcctgcca attaatggac atttcttttt
     1741 cctccgaaaa ataatcgcgg aatatcttct attctatttt aaaggataaa agtaaatcta
     1801 acaagtgtta atgtcaagtt tgtaaaagaa aattataatt gataagtgta ttatttttaa
     1861 gagtttttaa ccttatttta gatttgtgca aacagttatt gttttttgtc taaaaatgtt
     1921 ttaggataaa aatgtgatta tcatggttct agagtttgat aaaaaactac agaatataaa
     1981 gcgctttctt gaaaacaaca aaatcaatct tttaggagga gaaaatgaag aatccatttt
     2041 ttgaaagacg ttgtcgttac agtattcgta agttatcagt aggagcctgc tcgctgatga
     2101 ttggtgctgt tttatttgct ggtccagcct tggctgaaga aactgcagtt cctgaaaata
     2161 gcggagctaa tacagagctt gtttcaggag agagtgagca ttcgaccaat gaagctgata
     2221 agcagaatga aggggaacat gctagagaaa acaagctaga aaaggcagaa ggagtagcga
     2281 tagcatctga aactgcttcg ccagcaagca atgaagctgc aactactgaa actgcagaag
     2341 cagctagcgc agctaaacca gaggaaaaag caagtgaggt ggttgcagaa acaccatctg
     2401 cagaagcaaa acctaagtct gacaaggaaa cagaagcaaa gcccgaagca actaaccaag
     2461 gggatgagtc taaaccagca gcagaagcta ataagactga aaaagaagtc cagccagatg
     2521 tccctaaaaa tacagaaaaa acattaaaac caaaggaaat caaatttaat tcatgggaag
     2581 aattgttaaa atgggaacca ggtgctcgtg aagatgatgc tattaaccgc ggatctgttg
     2641 tcctcgcttc acgtcggaca ggtcatttag tcaatgaaaa agctagcaag gaagcaaaag
     2701 ttcaagcctt atcaaacacc aattctaaag caaaagacca tgcttctgtt ggtggagaag
     2761 agttcaaggc ctatgctttt gactattggc aatatctaga ttcaatggtc ttctgggaag
     2821 gtctcgtacc aactcctgac gttattgatg caggtcaccg taacggggtt cctgtatacg
     2881 gtacactctt cttcaactgg tctaatagta ttgcagatca agaaagattt gctgaagctt
     2941 tgaagcaaga cgcagatggt agcttcccaa ttgcccgtaa attggtagac atggccaagt
     3001 attatggcta tgatggctat ttcatcaacc aagaaacaac tggagatttg gttaaacctc
     3061 ttggagaaaa gatgcgccag tttatgctct atagcaagga atatgctgct aaggtaaacc
     3121 atccaatcaa gtattcttgg tacgatgcca tgacctataa ctatggacgt taccatcaag
     3181 atggtttggg agaatacaac taccaattca tgcaaccaga aggagataag gttccggcag
     3241 ataacttctt tgctaacttt aactgggata aggctaaaaa tgattacact attgcaactg
     3301 ccaactggat tggtcgtaat ccttatgatg tatttgcagg tttggaattg caacagggtg
     3361 gttcctacaa gacaaaggtt aagtggaatg acattttaga cgaaaatggg aaattgcgcc
     3421 tttctcttgg tttatttgcc ccagatacca ttacaagttt aggaaaaact ggtgaagatt
     3481 atcataaaaa tgaagatatc ttctttacag gttatcaagg agaccctact ggccaaaaac
     3541 caggtgacaa agattggtat ggtattgcta acctagttgc ggaccgtacg ccagcggtag
     3601 gtaatacttt tactacttct tttaatacag gtcatggtaa aaaatggttc gtagatggta
     3661 aggtttctaa ggattctgag tggaattatc gttcagtatc aggtgttctt ccaacatggc
     3721 gctggtggca gacttcaaca ggggaaaaac ttcgtgcaga atatgatttt acagatgcct
     3781 ataatggcgg aaattccctt aaattctctg gtgatgtagc cggtaagaca gatcaggatg
     3841 tgagacttta ttctactaag ttagaagtaa ctgagaagac caaacttcgt gttgcccaca
     3901 agggaggaaa aggttctaaa gtttatatgg cattctctac aactccagac tacaaattcg
     3961 atgatgcaga tgcatggaaa gagctaaccc tttctgacaa ctggacaaat gaagaatttg
     4021 atctcagctc actagcaggt aaaaccatct atgcagtcaa actatttttc gagcatgaag
     4081 gtgctgtaaa agattatcag ttcaacctcg gacaattaac tatctcggac aatcaccaag
     4141 agccacaatc gccgacaagc ttttctgtag tgaaacaatc tcttaaaaat gcccaagaag
     4201 cggaagcagt tgtgcaattt aaaggcaaca aggatgcaga tttctatgaa gtttatgaaa
     4261 aagatggaga cagctggaaa ttactaactg gctcatcttc tacaactatt tatctaccaa
     4321 aagttagccg ctcagcaagt gctcagggta caactcaaga actgaaggtt gtagcagtcg
     4381 gtaaaaatgg agttcgttca gaagctgcaa ccacaacctt tgattggggt atgactgtaa
     4441 aagataccag cctaccaaaa ccactagctg aaaatatcgt tccaggtgca acagttattg
     4501 atagtacttt ccctaagact gaaggtggag aaggtattga aggtatgttg aacggtacca
     4561 ttactagctt gtcagataaa tggtcttcag ctcagttgag tggtagtgtg gatattcgtt
     4621 tgaccaagcc acgtaccgtt gttagatggg tcatggatca tgcaggagct ggtggtgagt
     4681 ctgttaacga tggcttgatg aacactaaag actttgacct ttattataaa gatgcagatg
     4741 gtgagtggaa gctagctaag gaagtccgtg gtaacaaagc acacgtgaca gatatcactc
     4801 ttgataaacc aatcactgct caagactggc gcttgaatgt tgtcacttct gacaatggaa
     4861 ctccatggaa ggctattcgt atctataact ggaaaatgta tgaaaagctt gatactgaga
     4921 gtgtcaatat tccgatggcc aaggctgcag cccgttctct aggcaataac aaggtacaag
     4981 ttggctttgc agatgtaccg gctggagcaa ctattaccgt ttatgataat ccaaattctc
     5041 aaactccgct cgcaaccttg aagagcgaag ttggaggaga cctagcaagt gcaccattgg
     5101 atttgacaaa tcaatctggt cttctttatt atcgtaccca gttgccaggc aaggaaatta
     5161 gtaatgtcct agcagtttcc gttccaaaag atgacagaag aatcaagtca gtcagcctag
     5221 aaacaggacc taagaaaaca agctacgccg aaggggagga tttggacctt agaggtggtg
     5281 ttcttcgagt tcagtatgaa ggaggaactg aggacgaact cattcgccta actcacgcag
     5341 gtgtatcagt atcaggtttt gatacgcatc ataagggaga acagaatctt actctccaat
     5401 atttgggaca accagtaaat gctaatttgt cagtgactgt cactggccaa gatgaagcaa
     5461 gtccgaaaac tattttggga attgaagtaa gtcaggaacc gaaaaaagat tacctagttg
     5521 gtgatagctt agacttgtct gaaggacgct ttgcagtggc ttatagcaat gacaccatgg
     5581 aagaacattc ctttactgat gagggagttg aaatttctgg ttacgatgct caaaagactg
     5641 gtcgtcaaac cttgacgctt cgttaccaag gtcatgaagt caactttgat gttttggtat
     5701 ctccaaaagc agcattgaac gatgagtacc tcaaacaaaa attagcagag gttgaagctg
     5761 ctaagaacaa ggtggtctat aactttgctt caccagaagt aaaagaagct ttcttgaaag
     5821 caattgaagc ggccgaacaa gtgttgaaag accatgaaac tagcacccaa gatcaagtca
     5881 atgaccgact taataaattg acagaagctc ataaagctct gaatggtcaa gagaaattta
     5941 aggaagaaaa gacagagctt gatcgtttaa caggtgaggt tcaagaactc ttggctgcca
     6001 aaccaaacca tccttcaggt tctgccctag ctccgcttct tgagaaaaac aaggccttgg
     6061 ttgaaaaagt agatttgagt ccagaagagc ttgcaacagc gaaacagagt ctaaaagatc
     6121 tggttgcttt attgaaagaa gacaagccag cagtcttttc tgatagtaaa acaggtgttg
     6181 aagtacactt ctcaaataaa gagaagactg tcatcaaggg tttgaaagta gagcgtgttc
     6241 aagcaagtgc tgaagagaag aaatactttg ctggagaaga tgctcatgtc tttgaaatag
     6301 aaggtttgga tgaaaaaggt caagatgttg atctctctta cgcttctatt gtgaaaatcc
     6361 caattgaaaa agataagaaa gttaagaaag tatttttctt acctgaaggc aaagaggcag
     6421 tagaattggc ttttgaacaa acggatagtc atgttatctt tacagcacca cactttactc
     6481 attatgcctt tgtttatgaa tctgctgaaa aaccacaacc tgctaaacca gcaccacaaa
     6541 acaaagtcct tccaaaacct acttatcaac cggcttctga tcaacaaaag gctcctaaat
     6601 tggaagttca agaggaaaag gttgcctttc atcgtcaaga gcatgaaaat gctgagatgc
     6661 tagttgggga acaacgagtc atcatacagg gacgagatgg actgttaaga catgtctttg
     6721 aagttgatga aaacggtcag cgtcgtcttc gttcaacaga agtcatccaa gaagcgattc
     6781 cagaaattgt tgaaattgga acaaaagtaa aaacagtacc agcagtagta gctacacagg
     6841 aaaaaccagc tcaaaataca gcagttaaat cagaagaagc aagcaaacaa ttgccaaata
     6901 caggaacagc tgatgctaat gaagccctaa tagcagtctt agccagcctt ggtcttgcta
     6961 gtttagcctt gaccttgaga cggaaaagag aagataaaga ttaaatatcg aaaaatcttg
     7021 tgaaatcttt ccttatattt ccaaagtgtg atataatagt ttcgaataaa ataaataaag
     7081 gagtttttgt aacatggcaa aacttactgt taaagacgtt gacttgaaag gtaaaaaagt
     7141 cctcgttcgt gttgacttca acgtaccatt gaaagatggc gtaatcacta acgataaccg
     7201 tatcacagca gctcttccaa ctattaagta catcatcgaa caaggtggac gtgcaattct
     7261 tttctctcac cttggacgtg tgaaagaaga agctgataaa gctggcaaat cacttgctcc
     7321 tgtagcagct gacttggcag caaaacttgg tcaagatgtt gttttcccag gtgtcactcg
     7381 tggtgctgaa ttggaagcgg caatcaacgc tcttgaagat ggacaagttc tcttggttga
     7441 aaacactcgt tacgaagatg ttgacggcaa gaaagaatct aaaaacgatc ctgaacttgg
     7501 taaatactgg gcatcacttg gagatggtat cttcgtaaac gatgcattcg gtacagctca
     7561 ccgtgcacac gcatctaacg ttggtatctc agcaaacgtt gaaaaagcag ttgctggttt
     7621 ccttcttgaa aacgaaattg cctacatcca agaagcagtt gaaactccag aacgtccatt
     7681 cgtggctatc cttggtggtt caaaagtttc agacaagatc ggtgttatcg aaaacttgct
     7741 tgaaaaagct gataaagtcc ttatcggtgg tgggatgact tacacattct acaaagcaca
     7801 aggtatcgaa atcggtaact cacttgtaga agaagacaaa ttggatgttg cgaaagctct
     7861 tcttgaaaaa gcaaatggta aattgatctt gccagttgac tcaaaagaag ctaacgcatt
     7921 tgctggttac actgaagtgc gtgacactga aggtgaagca gtttctgaag gcttccttgg
     7981 tcttgacatc ggtccaaaat ctatcgccaa atttgacgaa gctttgactg gtgccaaaac
     8041 agttgtatgg aacggaccta tgggtgtatt tgaaaaccca gatttccaag ctggtacaat
     8101 cggtgtgatg gacgctatcg tgaaacaacc aggagttaaa tcaatcatcg gtggtggtga
     8161 ctcagctgcc gcagcgatta accttggccg tgcagacaag ttctcatgga ttagtacggg
     8221 tggtggagca tcaatggaac ttcttgaagg taaggttctt ccaggacttg cagccttgac
     8281 agaaaaataa gattttataa acaaatcaaa gaagagaggg atgaaagttc ctcttttctt
     8341 ttgcttaaaa taaaaacgct tcctctcaac tattactcat aaaaatcacc gatttatgat
     8401 aaaatggaaa tagaaagttg agattatgag ttattttaaa aaatataaat tcgataaatc
     8461 ccagttcaaa cttggtatgc gaacctttaa aacaggtatt gctgtttttc tagttctctt
     8521 gatttttggc ttttttggct ggaaaggtct tcaaattggt gctttgacag ccgtttttag
     8581 cctgagggag agttttgatg agagtgttca ttttgggact tcgcgtattc taggaaatag
     8641 tatcggtgga ctctatgcct tggtcttctt cttattaaat acttttttcc acgaagcctt
     8701 ttgggtgacc ttggtagttg ttccaatctg caccatgtta accattatga caaatgtagc
     8761 catgaataac aaagcagggg ttattggtgg tgtagcagct atgttaatca ttaccctatc
     8821 aattccaagt ggtgagacaa ttttgtacgt gtttgtgcgt gtattagaaa cgtttatggg
     8881 agtttttgtc gcaattatcg taaattacga tattgatcgt attcgtctct ttttagagaa
     8941 aaaagaaaaa taatgttaca ttttataaca ttatcaattg acgtttgtct ttttttagac
     9001 tataatagac agaaagaagg aaattgtaaa tgaaggaaaa agaatttcgc cgaaatatgg
     9061 ctgtttttcc tatcggcagt gttatgaagt tgaccgattt atcggcgcgt cagattcgtt
     9121 attatgaaga tcaagagttg atcaagcccg atcgaaacga aggaaatcgt cgcatgtatt
     9181 ccttgaatga catggatcgt ctgcttgaaa tcaaagatta tatctctgaa ggttataata
     9241 tcgctgccat taagaaaaaa tatgctgaac gtgaagcgaa atccaagaaa gcggttagtc
     9301 agactgaagt acgtcgtgca cttcacaatg aactcctcca acaggggcgt tttgcttcag
     9361 tacagtcacc ttttggtcgc ggttaggcaa ccgcaagtag tcatagatta aggagaaaac
     9421 ttatgccaat cacagctgca gatattcgtc gtgaagtcaa ggaaaaaaat gttaccttta
     9481 ttcgtcttat gttctcagat attttgggaa ccatgaaaaa cgtcgaaatt cctgctacag
     9541 atgaacagtt agataaggtc ttgtcgaaca aggttatgtt tgatggatct tctattgaag
     9601 gttttgtacg tatcaatgag tcggatatgt acttgtaccc ggacttggat acatggacag
     9661 tcttcccttg gggagatgaa aatggaagtg ttgcaggtct gatctgtgat gtctatacaa
     9721 cagaaggtga accatttgcg ggtgaccctc gtggtaattt gaaacgagct cttcgtcaca
     9781 tggaagaagt tggattcaaa tccttcaacc ttggtccaga gccagaattc ttcctattta
     9841 agttggatga aaatggggac ccaacacttg aagtgaatga caagggtggc tactttgatt
     9901 tggcacctac tgaccttgcg gacaacacac gtcgtgagat tgtgaatgtc ttgaccaaaa
     9961 tgggatttga agtagaagcg agtcaccacg aggttgcggt tggacagcat gagattgact
    10021 ttaagtacga tgaagttctc cgtgcttgtg ataagattca aatctttaag cttgttgtta
    10081 aaaccattgc tcgcaaacac ggactttacg caacatttat ggcgaagcca aaatttggta
    10141 ttgctggatc aggtatgcac tgtaatatgt ccttgtttga tgcagaagga aataacgcct
    10201 tctttgatcc aaatgatcca aaaggaatgc agttgtcaga aacagcttac catttcctag
    10261 gcggtttgat caagcatgct tacaactata ctgccatcat gaacccaaca gttaactcat
    10321 acaaacgttt ggttccaggt tatgaagcgc ctgtttacat tgcttgggct ggtcgtaacc
    10381 gttcgccact tgtgcgcgta cctgcttcac gtggtatggg aactcgtctt gagttgcgtt
    10441 cagtggatcc aatggcgaac ccttacattg ctatggctgt tcttttggaa gttggtttgt
    10501 atggtattga aaataaaatc gaagcaccag ctcctatcga agaaaatatc tacatcatga
    10561 cagcagaaga gcgcaaggaa gctggtatta cagaccttcc atcaactctt cacaacgctt
    10621 tgaaagcttt gacagaagat gaagtggtta aagctgctct cggagatcac atctacacta
    10681 gcttccttga agccaaacga atcgaatggg caagttatgc aaccttcgtt tcacaatggg
    10741 aaattgataa ttatttagac ctttactaat actaatatag aagaaagatt gcctgtgggt
    10801 aaacagtcac acagcagtct aagccatcaa atacctataa cccttaaaat agcgatttcg
    10861 gtcgctattt atttttcgaa aaatcctctt gactcttttg cgaattagat ttatcatcag
    10921 acgtgtagaa gaaaatcaag gagtcactat ggaaaaattc aatttcaaaa acaatatcgg
    10981 gcaagaaaac aagttacttc aaatcgaaat ctacaagttt actaactttt gcaagcttca
    11041 aaattacaca tctgtaaata ttttttcgaa agatatattt gaagccatag taaattgaga
    11101 ctagaatagt acactacatc tgctaaaaat ttctagaaat taatttaact ttcctaatcg
    11161 atttgtttac atcttatttc aatctactat attatctcaa taaatgatga tggcgactgt
    11221 tttgtttgtg cttgtcgatt tttgaatcct gatcgagcgg aagaatattt ctgccgaggt
    11281 tgtcaagggg aaaatcctga agatattgaa ttttatgatg aacaattaca ggcagaaaag
    11341 gtggaggctt tgaatattcg attggaagta aaaaattgaa ttatctacaa tctcatcaac
    11401 aataaaccta tttcaacccc attatctata ggcgtatttt tacgcctttt tatatacttg
    11461 aagcacataa gaaacattat gggaagtttg ctgatggaag tactcaagaa attgatgttc
    11521 cttatgatat tcctgatact tgggagtggg tgaggataaa atcaatttat tggaattttg
    11581 ggcaaaataa gccagagaaa tcctttaggt atatagatac gtctagtatt gatagaaaaa
    11641 agaacataat caactacaaa aatctacaat atctttcacc tgaacaagcg ccttcccgtg
    11701 ccagaaaatt agtttcgcag aatagtgtct tattttcaac agttagacca tatctaaaaa
    11761 atattgctgt agttagagaa cttaaagagt atttgatagc tagtacagca tttattgttt
    11821 tggatacttt acttaacgaa acatatttga aatattattt attatcagat aattttatca
    11881 atcgtgttaa taataaatct actggaacaa gttatcctgc aatcaatgat tataatttta
    11941 atctattatt aatt
//
LOCUS       DAVBHS010000054        11931 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVBHS010000054 DAVBHS010000000
VERSION     DAVBHS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11931
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.054"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(151..765)
                     /gene="lepB"
                     /locus_tag="VQ651_001055"
     CDS             complement(151..765)
                     /gene="lepB"
                     /locus_tag="VQ651_001055"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET2754063.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            complement(769..1650)
                     /locus_tag="VQ651_001056"
     CDS             complement(769..1650)
                     /locus_tag="VQ651_001056"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HET2754064.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQLVEENRGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TSDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            1737..2039
                     /gene="zapA"
                     /locus_tag="VQ651_001057"
     CDS             1737..2039
                     /gene="zapA"
                     /locus_tag="VQ651_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET2754065.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            2036..2584
                     /locus_tag="VQ651_001058"
     CDS             2036..2584
                     /locus_tag="VQ651_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET2754066.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIFYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            2688..5024
                     /locus_tag="VQ651_001059"
     CDS             2688..5024
                     /locus_tag="VQ651_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HET2754067.1"
                     /translation="MNKKILETLEFDKVKALFDPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     regulatory      5188..5276
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            5367..6689
                     /locus_tag="VQ651_001060"
     CDS             5367..6689
                     /locus_tag="VQ651_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HET2754068.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVFFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     gene            6907..7455
                     /locus_tag="VQ651_001061"
     CDS             6907..7455
                     /locus_tag="VQ651_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET2754069.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            7565..8464
                     /locus_tag="VQ651_001062"
     CDS             7565..8464
                     /locus_tag="VQ651_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HET2754070.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACSGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(8489..9763)
                     /gene="serS"
                     /locus_tag="VQ651_001063"
     CDS             complement(8489..9763)
                     /gene="serS"
                     /locus_tag="VQ651_001063"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET2754071.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGTDEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(9977..10348)
                     /locus_tag="VQ651_001064"
     CDS             complement(9977..10348)
                     /locus_tag="VQ651_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HET2754072.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(10351..11715)
                     /locus_tag="VQ651_001065"
     CDS             complement(10351..11715)
                     /locus_tag="VQ651_001065"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET2754073.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
BASE COUNT         3290 a         2482 c         2619 g         3540 t
ORIGIN      
        1 ctatccacaa cctcaaaaca ctgttttaag cagcctacgg ctagcttcct agtttgttct
       61 ttgattttcg ttgagtattt gtcaataaac aatcacttct cacgatagaa gaagaggctg
      121 agattggtga ttctcggcct cttaggtctc ttaaaatgtt ccgatacggg tgattggcca
      181 gaagcggaat ttagcttccc ctgtgatatc ttttgctttg aaggtaccta cgtggcggct
      241 gtcgctcgaa accaagcggt catctccgag gagaaggtat tctccttctg gaacagtaaa
      301 gctaaagttg gtgttgtagt tgacatcaac tgtgaaggct tgggcttttt gagcgatact
      361 tctaaagaaa gttcctttat ttccttcaaa gcccttgcct gagtaggtgc tttggagttt
      421 gtcatccttg aagcgtttga tatagtctgc tagataaggc tcgtccgttt ctttgtcatt
      481 gatgtagagt ttatcatttt cgtaacgaat ggtgtcgcca ggcattccaa tcacgcgctt
      541 gacgatgtcc ttattgccat cttcctcatg ggccaccacg atatcaaaac ggtcaatagg
      601 aaggtgtttt acaacgaaga gaatttcgcc atccgctagg gtcggatcca tggaatgtcc
      661 ttctacgcga acattgctcc aaaaaaagat acgacttaaa gctagtaatg acagaattag
      721 gaggaacaat ccccactctt ttaagaaatt tttaaatgaa ttcataactt acctttctaa
      781 gcgttttttc gctttttcag tgtttttaaa gtgcaatttg gcgcagaagc tgagtccctg
      841 cataccatag gcttgcaaaa tctggctagc caccttgtca gaagccgttc cagctccact
      901 tgggagctga taacccagtt ctcgtcccaa attttcaaga ttttccagaa agagatcacg
      961 cgcaatgaca gaagaaactg cgacagacaa gtatttgccc tcagcctttt cttctaagct
     1021 gataggattg ctgaaacgat tggcctcttg tgccaagtac ttgtcataat ttttagcact
     1081 ggtaaaggca tcaatcacaa ttttctcagg ctgaacacct ttttgaagga ggagatagat
     1141 agcctgatta tggagggcaa ccttaaccga aacagcattg tagcggtctc cgatgacctc
     1201 gttgtacttg ctgggtgaga gaaggagtgc ctggtgctgg attttttcct tgagaatagg
     1261 agcaatctga cggatctttt ggtcggtcag agtcttagaa tcccccacac cgagttttcg
     1321 taaaaagtcg tgctggtcag atgtgacaaa ggcagccaca actgcaagcc caccaaagta
     1381 ggaaccattt cccacctcat ctgtcccaat taaaggaaga ttttgccccc tgttttcctc
     1441 tacaagttga tagccaaaga aactagcgta tttctcagcc ccttcaccct gaagcaagat
     1501 ttttccagaa gtatagatag aaaccgttgc ttgaggtagt ttcaaaaagt agcggatata
     1561 gggattctta ctgggagcca gactggtttg atagtgttca agaaaagcct gaatatcctt
     1621 ttcgcttggt gtgagtgtga tacttgccat agtttctatt gtaccacaaa agcagtaaaa
     1681 tttgtaaaaa ctgacaaaat tagcgaattt tggtataata tcgtgaggtg aattttatgg
     1741 caaatctaaa tcgattcaaa tttacattcg ggaaaaaatc gttaaccttg acaagcgaac
     1801 atgataacct ttttatggag gaaatcgcta aggttgcgac agaaaaatac caagcaatta
     1861 aagaacaaat gcctagcgca gatgatgaaa caatcgctct tttgttggca gtcaactgtt
     1921 tatcaactca gctcagccgt gagattgaat ttgacgataa ggagcaagag ctagaagaac
     1981 tccgtcacaa gcttgtgact tgtaagcaag aacagagcaa gattgaggat tccttatgat
     2041 ttcattcctt cttctattgg tcttggtttg gggattttat atcggctatc ggagaggcct
     2101 gctcttacag gtttattacc tgatttcagc catggcatcg gcttttatgg ctggccagtt
     2161 ttataagggg cttggagagc aattccattt attgctccct tatgcaaatt cgcaggaagg
     2221 tcaggggact ttctttttcc catcggatca actctttcag ctggataagg tcttttatgc
     2281 aggtatcggc tacttgcttg tatttgggat tttctatagc attggtcgtt tgcttggtct
     2341 tctcttacac ttgattccta gcaaaaaact gggtggtaag ttgttccaag tttcagcagg
     2401 tatcttgtcc atgttggtga ccttatttgt cttgcaaatg gccttgacaa tcttggcgac
     2461 catccccatg gcagttatac aaaatcctct tgaaaagagt atcgtcgcaa aacacatcat
     2521 ccagagcata ccgataacaa ccagttggct caaacaaatc tgggtgacaa atttaatcgg
     2581 ataaaaaggg caggagtttt cctagccctt tgtttacaga tttgactcga atctatcaga
     2641 atgtaaaaag ctaccacacc tagacattca aagacaagga aataaagatg aataagaaaa
     2701 tattagaaac attagagttc gataaggtca aggccttgtt tgatcctcat ttgttgaccg
     2761 agcagggctt ggagcaattg agacagctgg ctccgactgc caaagcagat aaaatcaaac
     2821 aggcttttgc tgagatgaaa gaaatgcagg ctcttttcgt cgagcaaccg cattttacta
     2881 ttctctcaac taaggaaatt gcaggagtct gcaagaggtt ggagatggga gcggatctca
     2941 atatcgagga gttcctactc ttgaaacgcg tgcttcttgc cagccgagaa cttcaaagtt
     3001 tttatgccaa tctggaaaat gtcagcttgg aagaattagc cctttggttt gagaaattac
     3061 atgattttcc gcaattacaa ggaaatcttc aggcctttaa tgatgcgggt ttcattgaaa
     3121 attttgccag tgaagaattg gcgcgaatcc gtcgaaaaat acatgatagc gagagtcagg
     3181 tacgcgatgt tttacaagac ttgctcaagc aaaaagcgca gatgttgacg gaaggaattg
     3241 ttgctagcag aaatggccgt caggttttac cagtcaaaaa cacctaccgc aataagattg
     3301 caggtgtcgt tcatgatatt tctgctagtg gaaacaccgt ctatatcgaa ccacgtgagg
     3361 tagtcaaact gagcgaagaa attgctagtc tgcgagcaga tgagcgctat gaaatgcttc
     3421 gcattctcca agaaatttct gagcgtgtcc gccctcatgc ggctgagatt gctaatgacg
     3481 cttggattat cggtcatctg gacttgattc gtgccaaggt tcgatttatc caagaaagac
     3541 aagcagtcgt gcctcagctg tcagaaaatc aagagattca actgctccat gtctgccatc
     3601 ctttggtcaa aaatgccgtc gcaaatgatg tccattttgg tcaagattta acagctattg
     3661 tcattacagg tcccaataca ggtgggaaga ccatcatgct caaaactctg ggcttgacac
     3721 aggtcatggc ccagtcagga ttgccgattt tagcagacaa gggaagtcgt gttggtattt
     3781 ttgaagaaat ctttgctgat attggagatg agcagtctat tgagcagagc ttgtctacct
     3841 tctctagcca tatgaccaat atcgtggata ttcttggcaa ggtcaaccaa cattcactct
     3901 tacttttgga tgagttgggg gctggtactg atccccaaga gggagcagcc cttgccatgg
     3961 ctattctgga ggaccttcgc ctgcgtcaaa tcaagaccat ggcgacgacc cactatccag
     4021 aactcaaggc ctacggtatt gagacagcct ttgtgcaaaa tgccagtatg gagtttgata
     4081 ctgcaactct tcgcccgacc tatcgcttta tgcagggtgt tcctggccga agtaatgcct
     4141 ttgaaattgc caaacgtcta ggcctatctg aagttatcgt aggagatgcc agtcagcaga
     4201 tcgatcagga caatgacgtc aatcgtatca ttgagcaatt agaagagcag acgctggaaa
     4261 gccgcaaacg tttggacaat atccgtgagg tggagcaaga aaatctcaag atgaaccgtg
     4321 cgctaaaaaa actctacaac gagcttaatc gtgaaaagga aaccgagctt aacaaggcgc
     4381 gtgaacaggc tgctgagatt gtggatatgg ccctaagtga aagtgaccag attctcaaaa
     4441 atctccacag taaatcccaa ctcaagcccc acgaaatcat tgaagccaag gccaagttga
     4501 aaaaattggc tcctgaaaaa gtggacttgt ctaaaaataa ggtccttcaa aaggccaaga
     4561 aaaaacgagc tccaaaggtg ggagatgata tcgtggttct cagttatggt cagcgtggta
     4621 ccttgaccag tcaactcaag gacggtcgct gggaagccca agttggcttg attaagatga
     4681 ccttggaaga gaaagagttt gatcttgttc aagcccagca agaaaaacca gtcaagaaga
     4741 aacaggtcaa tgttgtgaaa cgaacttctg ggcgaggacc tcaagctaga ctggatcttc
     4801 gaggcaagcg ctatgaagaa gccatgaatg agctagatac cttcatcgac caagccttgc
     4861 ttaacaatat ggctcaagtt gatatcatcc atggtatcgg aacaggagtc atccgtgaag
     4921 gagttaccaa atacttgcaa agaaacaaac atgtcaagag tttcggctat gccccacaaa
     4981 atgctggagg cagtggtgcg actattgtca cttttaaagg atagcagtat tctggacttt
     5041 ataaagtaaa aactgttgaa ctaattttta ctaataaaca cattgacaaa agccaacatt
     5101 ttttgtaaaa ttagaatcaa ttaaatacca acaccgaatg aagtttaata gaagtgggga
     5161 atcgtttgat tttccatgac tgtaaatgga cggaactctg gagagaccgt aaaggcaccg
     5221 aaggggcaag gcaggcaact gctcaaactc tcaggtaaaa ggacagagct aggatagacc
     5281 gctttttagc atttatctaa gcattccaga gtacatgtat cttgcatgtg ctctttcttt
     5341 tggggttgaa acgataggag aaggaaatgt tagaattgct taaatcaatc gatgcttttg
     5401 cttggggacc gcccctcttg attttattgg tcggaacagg gatttaccta accatgcggc
     5461 taggactctt gcaggttttg cgtctgccca aggcctttca gcttattttt atccaggata
     5521 agggacatgg tgatgtatcc agttttgcag ctctgtgtac agccttggca tcaactgttg
     5581 gaacaggaaa tatcatagga gttgcgacgg ctatcaaggt tggtggacca ggagctctat
     5641 tttggatgtg gatggcggct ttctttggaa tggctaccaa gtatgcggaa ggactcttgg
     5701 ccatcaaata ccgcaccaag gacgaccatg gtgcagtagc gggaggtccc atgcactata
     5761 tccttctagg gatgggagaa aagtggcgac cacttgctgt tttgtttgca gtagcaggag
     5821 tattggttgc tctcttggga atcggaacct tcacccaagt caactcgatt acagaatcta
     5881 tccaaaatac aacgacgatt tcgccagcca tcacagctct cgtcttgtct gtctttgtag
     5941 cgattgcagt ctttggtgga ctcaagtcta tttctaaggt ttcaactact gttgttcctt
     6001 ttatggccct catttatatc ttaggaactc ttacagttat tttctttaat atcggaaaaa
     6061 tccctggcac aatcgcttta gtctttacct cagcttttag tccccttgct gcggtaggtg
     6121 gatttgctgg tgctagcgtt cggatggcta ttcaaaatgg tgtggcgcgt ggtgtgttct
     6181 caaacgaatc tggtctgggt tctgctccta ttgcagctgc agctgccaag acaaatgaac
     6241 cagtagagca aggtttgatt tccatgacag gaacctttat tgataccctc atcatttgta
     6301 ctctaactgg tttgaccatc ttggtaactg gagtttggag tggtgacttg aatggggttg
     6361 ccttgactca gtcagctttc tcaacagtct tttcacactt tgggcctgcc ctcttgacca
     6421 tcttccttgt actttttgcc tttacaacga ttctaggttg gaactattac ggagaacgct
     6481 gttttgagtt cctctttggg gctcgcttta tctggctcta ccgtgtgttt tttgtgctca
     6541 tggtcttgtt aggaggattt atcgagttgg atatggtctg gattatcgca gatatcgtta
     6601 acgccttgat ggctctgcca aacttgattg ccctcttggt cttgtcgcca gtcgttattg
     6661 ctgaaactaa aaagtatttt gacaaataat ggaatcacac agctcgtgtg atttttttgc
     6721 ctataaataa tttttatcag cctgatatat aatatatatt atgcaagtga ttggaagtgt
     6781 gataaactaa tataacaacg aaaatcttat gagagaataa agctttatct tagataaact
     6841 taggaatcag gattttataa gattttccaa taatattagt gtcaataata agaaaagagg
     6901 tatcttatga caacatttac tatccataca gtagagtcag caccagcaga agtgaaagaa
     6961 attcttgaaa cagtagaaaa agacaacaat ggctatattc ccaacctaat cggtctcttg
     7021 gccaatgccc cgactgtttt agaagcctac caaattgtct catctatcca ccgtcgcaac
     7081 agcctgacac ccgttgagcg tgaagtggtg caaatcacgg cagccgtgac caatggttgt
     7141 gccttctgtg tcgcaggtca cacagccttt tccatcaaac aaatccagat gaatgatgac
     7201 ttgattcaag ctcttcgcaa tcgtactcca attgaaacag atcctaaatt ggatacccta
     7261 gctaagttta ccttggcagt tatcaatacc aagggtcgtg taggagatga agccttgtct
     7321 gagtttttag aagctggcta cactcaacaa aatgccttgg atgtggtttt tggtgtcagc
     7381 ctagcaatcc tctgtaacta tgccaacaac ttagctaata caccaattaa tccagaattg
     7441 caaccttatg cctaattcat atctgagtaa aatgaagtct gaaataagcg gacttcgttt
     7501 ttttaaatcc tcatttaagc gcttttatgt tatactgaaa ctaacatgat tattggaggt
     7561 taggatgaaa aaactcccct tagtattttc aggttgtttg ctaggtttgg caggagctgg
     7621 aaatcttatt ttagatacgt tgccggttct atcccatctt tttagtctga ctggtttggt
     7681 tttatggatt tactttctaa ttctgcatct ctttaattgg aaagaaacca agcaagaatt
     7741 gaccaagccc cctcttttgt caggaatggc aacctttcct atggctggga tgattttatc
     7801 gacctatgtc tttcgcgtat tctcttatct tcctttggta gcacaaggga tttggtggtt
     7861 ttcatttctc ttggatttga ccttgattgc tggttttacc atcaagtttg cttgttcagg
     7921 gcggagggtt catgccactc caagttggac ggttctctat gtggggatag cagtggctgc
     7981 cttgacctat cctctggtag gtattatcga aattgcctat gcgaccttga gttttggttt
     8041 tctcttgacc ttctatctct atccccttat ttatagcgat ttaaagaaac atccactccc
     8101 actagccttg cttggacaag aaggaatcta ctgtgctcct ttctctctac tcttggcttc
     8161 tctagttcga gtaggaggaa ccagcctgcc gacttgggtc ttgattgtca tgattttggc
     8221 ttctcaatcc ttctttttct ttgttttaac tcgtctgccc aacattttaa aacaaggttt
     8281 tcaaccagcc ttctcagccc tcaccttccc aaccattatc acagcgacct cgctcaagat
     8341 ggctcaggga attttgaaac ttccatttct ggattacctg gtattggctg aaaccattat
     8401 atgcctaact attttattct ttgtactagg tgcttatctg atttggttac gaaaaaaggt
     8461 ctagctagaa atagctaaac cttatttttt atggtttgat gacttcagct ccacccatgt
     8521 atggacgaag tgcttctggg atggtcacag aaccatcttc attttggtaa ttttcaagaa
     8581 ttgcagccac tgtacgtcca actgcaagtc cagagccgtt caaggtatga aggagtttca
     8641 ccttgccatc tgcttcatca cggtaacgga tttgggcacg acgggcttgg aaatcttctg
     8701 tgtttgaaca gcttgagatt tcacggtaat tgttttgtgc tggaatccac acttccaagt
     8761 cgtaagtctt cgcagctgag aagcccatat ctccagtaga gagagcaacg acacggtatg
     8821 gaaggttgag tttttgaaga atgttttcag cgttggctgt cattttttcc aattcttcgt
     8881 aagattcttc tggtttggca aatttgacca tttcaacctt gtggaattgg tgcaaacgga
     8941 tcaagccacg cgtatcacga ccggcagaac cagcctcaga acggaatgac ggactcatgg
     9001 cagtgaagta gattggaaga tctttgccgt ctaagatttc atcacggtag tagtttgtca
     9061 gaggaacttc agcagttgga atcaagacaa agttggtatc gctgagttca aaagtatctt
     9121 ccttaaattt tggatactga ccagtaccaa acatagaatc atggttgact atgtaaggtg
     9181 tgatgacttc agtatagcct tcttttccat gttcatccaa cataaagttg tagatagcac
     9241 gttccaaacg agcaccgagg cctttataga agaggaagcg agcgcctgtt accttaccac
     9301 cgcgttccca gtcaaggata ccaaggtctt caccgagatc ccagtgagct ttaggttcga
     9361 agtcaaactc gcgtggagta ccccaacggc gaacttccac attgtcgtct tcgtcagtcc
     9421 caacaggaac gctgtcagct gggatatttg gaagagtcgt tgtaaattct gtcaatttag
     9481 catcgatttc tgccaattca gcatccaagg ctttaacctc agcagataga ttttgcatgg
     9541 cagcaatctt gtcatctgta ttttccttgt tgcgcttagc ttgggcaatc tcagcagaaa
     9601 ctgtgttacg ttctgctttg agagtttcaa ccttgaccaa gatgttacga cgtttagcat
     9661 cgatttcttt catttcattc aagacagcag catctacacc acgtgtagct aatttttctg
     9721 cgacagcttc aaaatctgta cgaatacgtt tgatatctaa cataagaact cctttatgaa
     9781 aaaagcacgc ctgacaaaac gttggagtgg cagggccacg gttccatcca acttcacagg
     9841 tgtgcacttg attgtgtatg taattgttac taacggtaga atttcaccta tcccccctat
     9901 ctgctcgcag tacccgcaga ctttctgaaa gaagaagata acctacttat ccgttgctat
     9961 gattatacta aagtttctac ttttttgcaa atagattttt aaatttttgg ctaattgtct
    10021 gaatcagggt cggaagtttg acgaccttgt cattgcctag tttttcgcgt gcaattttga
    10081 gaatggcacc tgagtctttt gaagcaaaga ggaattttcc tttgtctgta aagacttcga
    10141 agtggcggct gattttgcgt ccagtgacat tggctccaat ctgattgata tggctccaag
    10201 gaatctggat aaattgttcg acattgacat ctgggtaaaa ttccaaagcc tgatctccga
    10261 caaggaattt cccaactttc ccagcgatag agaggtagga agtgcctgtc gtactgagga
    10321 gtactgtttt gttaagtgat tgggccatgc ttagtcttcc ttactttctc caaaaaaggc
    10381 attgtagagg gctttaattg ctgctttctc ttggtcttta ttgacaacaa acataataga
    10441 aacttcacta gaaccttgag acatcatctg gatgttgatt ttgttttcag atagagcgcg
    10501 tgtcgcagta gcagtcactc cgatatggct cttcattttt tcaccaacaa tcataatgat
    10561 agaaaggtcg tgttcgattt ctgcatgatc tactttagcc ttttgaacca actgacgcag
    10621 gatttcttct tccttgatgg gagttagttg gcgagaacgg agaatgatag aaagatcgtc
    10681 gatacctgtt ggcatatgtt cccaaccgat gttaagttct tccaggattt gcagaacctt
    10741 gcggccaaat ccaacctcac ggttcatgag gtatttcgac atgttaatgc tgacaaagcc
    10801 tgagtcacca gcaattccca caactggaaa ttcatcatta ctgtgtttta gaacgatacg
    10861 agtacctgga tggtcagggt tgttggtatt cttgataacc agaggaattt ttccacggta
    10921 ggcaggaaga agagcctcgt catgaaggac tgagaagcct gcataggcca actcgcgcat
    10981 ttcacggtag gtcaactcag gaatcgagtg tggttggtgg ataataccag ggtgggctgc
    11041 aaagatacca tcaacgtccg taaagttttc atagaggtca gctttgacac cagcagcaat
    11101 gatagaacct gtaatatcag aacctccacg tgagaaagta cagatttgat tttccttagt
    11161 gacaccaaag aaaccaggaa tgacaaggac ttcattggtg tttgtcaatt cttcaatctt
    11221 gtcataactt gatggaatga tgcgagcgtg accaggttca cttgtgacca caatcccagc
    11281 ttctctaggg tgcatatagc gtgcatcgat accattttgg ttaaagtagg cagcaatcaa
    11341 tttggcattg ttattttcac cggctgctag gaaagtatcg tagagaaatt cattttcttc
    11401 aataggaaga gtggccaagg cgtgaatgct tttagaaatt ttttctagca cagctggttt
    11461 tagtcccaat tcactaacca tagcagcata gcggtcgata atccagcttt ggctcttgct
    11521 aatatcgtta cccgcaacat agtcgcggta gtatttaatc agggcatccg taaccttagt
    11581 atcttcagca ttgcgtttac caggcgcaga aacgactaca aaacgacgct ctgaatcgct
    11641 tttgacgatg tttaaaactt tttctaattg actagcagag gcaagagaac tacctccaaa
    11701 tttaacaact ttcataagaa ctcctaaagt aagtatttta tacgattata gcagaaagaa
    11761 aggctttttt caatgaagaa aataagatgc tttctagcct aaagcgagcc tttcttatcg
    11821 gctgagacac tttcagtcga ttttttcttg ctttctgctc ctaaaaaaga tatactttga
    11881 cagtgaaata atttaacact caatgaaaat caaagagcaa actaagaagc t
//
LOCUS       DAVBHS010000055        11745 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVBHS010000055 DAVBHS010000000
VERSION     DAVBHS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11745
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.055"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            171..2462
                     /gene="pcrA"
                     /locus_tag="VQ651_001066"
     CDS             171..2462
                     /gene="pcrA"
                     /locus_tag="VQ651_001066"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HET2754074.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTTYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2505..3185
                     /gene="radC"
                     /locus_tag="VQ651_001067"
     CDS             2505..3185
                     /gene="radC"
                     /locus_tag="VQ651_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET2754075.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3182..3871)
                     /locus_tag="VQ651_001068"
     CDS             complement(3182..3871)
                     /locus_tag="VQ651_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET2754076.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYNLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(3889..4530)
                     /locus_tag="VQ651_001069"
     CDS             complement(3889..4530)
                     /locus_tag="VQ651_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET2754077.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(4613..4828)
                     /locus_tag="VQ651_001070"
     CDS             complement(4613..4828)
                     /locus_tag="VQ651_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET2754078.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(4836..5183)
                     /locus_tag="VQ651_001071"
     CDS             complement(4836..5183)
                     /locus_tag="VQ651_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HET2754079.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFSLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5188..6303)
                     /locus_tag="VQ651_001072"
     CDS             complement(5188..6303)
                     /locus_tag="VQ651_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2754080.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6313..7272)
                     /locus_tag="VQ651_001073"
     CDS             complement(6313..7272)
                     /locus_tag="VQ651_001073"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET2754081.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7385..7954)
                     /locus_tag="VQ651_001074"
     CDS             complement(7385..7954)
                     /locus_tag="VQ651_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HET2754082.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAEDYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8082..8753
                     /locus_tag="VQ651_001075"
     CDS             8082..8753
                     /locus_tag="VQ651_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HET2754083.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            8737..9555
                     /locus_tag="VQ651_001076"
     CDS             8737..9555
                     /locus_tag="VQ651_001076"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HET2754084.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            9552..10448
                     /locus_tag="VQ651_001077"
     CDS             9552..10448
                     /locus_tag="VQ651_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2754085.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            10492..11466
                     /gene="pta"
                     /locus_tag="VQ651_001078"
     CDS             10492..11466
                     /gene="pta"
                     /locus_tag="VQ651_001078"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HET2754086.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT         3416 a         2253 c         2528 g         3548 t
ORIGIN      
        1 gtattgagct agtttatgaa aaaattgggt gaaaaagtcg agtgttttag aaacccacag
       61 tgtagtattc tagtttcaat ccactatatt ttgctactcc ccgtaaagtt tctattttcc
      121 ctgatttctg atataataga aatattgact tcaagagtaa ggaagagaag atgaacgcat
      181 tattaaatgg aatgaatgac cgtcaggctg aggcggtgca aacgacagaa ggtcccttgc
      241 taatcatggc aggagctggt tctggaaaga ctcgtgtttt aacccaccgt atcgcttatt
      301 tgattgatga aaagctggtc aatccttgga atatcttggc cattaccttt accaacaagg
      361 ctgcgcgtga gatgaaagag cgtgcttata gcctcaatcc agctactcag gactgtctga
      421 ttgcgacctt ccactccatg tgtgtgcgta ttttgcgtcg cgatgcggac catattggct
      481 acaatcgtaa ttttacaatt gtggatcctg gtgaacagcg aacgctcatg aaacgtattc
      541 tcaaacagtt gaacttggat cctaaaaaat ggaatgaacg aactattttg gggaccattt
      601 ccaatgctaa gaatgatttg attgatgatg ttgcttatgc tgctcaagct ggcgatatgt
      661 atacgcaaat tgtggcccag tgttatacaa cctatcaaaa agaacttcgt cagtctgaat
      721 ccgttgactt tgatgatttg attatgctga ccttgcgtct ctttgatcaa aatcctgatg
      781 ttttgaccta ctaccagcaa aaattccaat acatccacgt tgatgagtac caagatacca
      841 accacgctca gtaccaattg gtcaaactct tggcttcccg ttttaaaaat atctgtgtgg
      901 ttggggatgc ggaccagtct atctacggtt ggcgtggtgc tgatatgcag aatatcttgg
      961 actttgaaaa ggattacccc aaagctaagg ttgttttgtt ggaggaaaat taccgctcaa
     1021 ccaaaaccat tctccaagcg gccaacgagg ttattaaaaa taataaaaat cgccgtccta
     1081 aaaatctctg gactcaaaac gctgatgggg agcaaatcgt ttactatcgt gctgatgatg
     1141 agctggatga ggctgtattt gtagccagaa ccatcgatga acttagtcgc agtcaaaact
     1201 tccttcataa ggattttgca gttctctatc ggactaatgc ccagtcccgt acaattgagg
     1261 aagccctgct caagtctaac attccttata ccatggttgg cggaaccaaa ttctacagcc
     1321 gtaaggaaat tcgcgatatt attgcttatc tcaaccttat tgctaatttg agtgacaaca
     1381 ttagttttga gcgtattatc aacgagccta aacgtggaat tggtctaggt acagttgaga
     1441 aaatccgtga ttttgcaaat ttgcaaaata tgtctatgct ggatgcttct gctaatatta
     1501 tgttgtctgg tatcaagggt aaggcagccc aatctatctg ggattttgcc aatatgatgc
     1561 ttgatttgcg ggagcagcta gaccacttaa gcattacaga gttggttgag tccgtcctag
     1621 aaaaaacagg ttatgtcgat attcttaacg cccaagcgac tctagaaagc aaggcaaggg
     1681 ttgaaaatat cgaagagttt ctttctgtta cgaagaactt tgatgacacc actgatgtga
     1741 cagaagagga aactggtctg gacaaactga gtcgtttctt aaatgacttg gctttgattg
     1801 ccgacacaga ttcaggtagt caggagacat cagaagtgac cttgatgacc ctgcatgctg
     1861 ccaaaggtct cgaatttcca gttgtctttt tgattgggat ggaagaaaat gtctttccac
     1921 ttagtcgtgc gactgaagat ccagatgaat tagaagaaga gcgccgtcta gcctatgtag
     1981 gtatcacgcg tgcagagaaa attctctatc tgaccaatgc caactcacgc ttgctttttg
     2041 gtcgtaccaa ttataaccgt ccgactcgtt ttattaacga aatcagttca gacttgcttg
     2101 agtatcaagg tctggctcgt cctgcaaata caagctttaa ggcatcatat agcagtggta
     2161 gtatttcctt tggtcaaggt atgagtttgg ctcaggctct tcaagaccgt aaacgcggtg
     2221 ctgccccaaa atcgatccag tcaagcggtc ttccatttgg tcaatttaca gctggcgcaa
     2281 aatcagcatc tagcgaggca aattggtcca ttggtgacat tgctctccac aagaaatggg
     2341 gagagggaac tgttctggaa gtttcaggta gcggtgctag gcaggaattg aaaatcaatt
     2401 tcccagaagt aggtttgaaa aaacttttag ccagtgtggc tccaattgag aaaaaaatct
     2461 aattttccat ccttctcacg aataataaag tgaggaggat ttttatgtac agtatttcat
     2521 tccaagaaga ttcactatta ccaagagaaa ggctggccaa ggaaggagtt gaagcgctta
     2581 gtaaccaaga gttgctagct attttactca ggacaggaac acgtcaagct agcgtttttg
     2641 aaattgccca aaaagtcttg aacaatcttt caagcctaac ggatttgaaa aaaatgaccc
     2701 tgcaggaatt gcagagtttg tctggtattg ggcgtgttaa ggccatagaa ttacaagcta
     2761 tgattgaact ggggcatcgt attcacaaac acgagactct tgaaatggaa agtattctca
     2821 gcagtcaaaa gttggccaag aagatgcagc aggaattagg ggataaaaaa caagagcacc
     2881 tggtggcact ctatctcaat actcaaaatc aaatcatcca tcagcagacc atttttatcg
     2941 gttctgtaac tcgtagtatc gctgaaccgc gagagattct tcactatgca atcaagcata
     3001 tggcgacttc tcttatcttg gtccacaatc atccttcagg agcggtagcg cctagccaaa
     3061 atgatgatca tgtcactaaa cttgttaaag aagcctgcga attgatgggg attgttctct
     3121 tggaccattt gattgtctct cattctaatt actttagtta tcgtgaaaag acagatttaa
     3181 tctaaagttc attaacgaca tagtcaaaga gttttttatc tttgggacga ttttcaaaaa
     3241 gaagttctgg atgccattgg acaccgagaa aggcgacatc atccgtactc atgacagcct
     3301 caatgatacc atctttagga tcatgagcca caacttttaa atttggtgct aagtccttga
     3361 tgctctggtg gtggaaggag ttgatatgag agatttctcc atagatttct tggagaacgg
     3421 tatctggttc tgttaccaag cgttgagttg tgtactcaac agaagaatcc tgccaatggt
     3481 cttcgatatc ttggtacaaa gttccaccca tggcaacgtt aaagagttgg gtaccacggc
     3541 agacagagaa aatgggcttt ttctgtttaa tagcttcctt gatgagggcc agttcgaaga
     3601 tatctctttg aaggttatag tcatcgctat caatggtttt gggttcgcca taaaattttg
     3661 gatcgacatt ttgcccacct gtcaagatga gcttgtcaat caaactgata tagtggcagg
     3721 ccatttcttg atcaccaatc ggtaggatga tgggaatccc tccagcatct ttaacgcctt
     3781 caacaaagcc ttttgctgcg tagctcatca tgatgtcatc atctggatga gctttttcgt
     3841 ttcctgtaat cccaataact ggttttttca taaaatgatt ttcgctttct aatcctcttt
     3901 tcgcatgaag tagaggaggg tttggagttc acttgtcaaa tcgacatact gaacgaccac
     3961 gtcttttggt aaatgcagat ggactggtga aaaactgaga attcctttca caccagcatc
     4021 aaccaagaga ttagcaacct cttgtgactt gacgctggga acagttagga tagcagtctt
     4081 cacatcagca tccttgattt tatccttgat ctgagaaatc ccgtaaatgg gaatcccgtc
     4141 aggagtttgg gtaccgactt caggatggtc gtctaggtca aaggccatga taatcttcat
     4201 cttgttacgt tcgtggaagc ggtagtggag aagggcatgg cccatatttc caataccaac
     4261 cagcatgaca ttggtaatag agttgtcatt gagcaaatcg gcaaaaaatg tcattagttt
     4321 tttgacatca tagccaaaac cacgacgacc aagttcacca aaataggaaa aatcacgacg
     4381 tacggtcgct gaatcaatac cgatagcctc tgcaatttgc ttagagttgg cacgttcaat
     4441 cttttctgca tgaaatctct taaaaattcg atagtagaga gagagtcttt ttgctgtagc
     4501 ttttggaata gcaaactgtt tatctttcac aaaatcacaa cctttctatt cttctatttt
     4561 atagaaacat tgtgaaaaaa tcaacaaaaa taagaaaaaa ctaagaaaaa tcttagtttt
     4621 gatgtaaaaa atctgcatga gatagaaaac ggtagaggtc tccgaccaga ccctgataaa
     4681 ctttttggcc cctaaaagtc agagaagtca cataaagtgt atctggtaag gttacacatc
     4741 ctgacaaagt caacatgaga gcctcatgat cctcatactt gagagtacgc tctacatgat
     4801 agcagtcctt ataggtcagt tcaaacattt tggctctatc tttccgattt tgtaaagaca
     4861 ccacgttcta ccaagctatc catgaggaag tagaattttt cctgatgaat atggtggtct
     4921 tctgatttga aaatatcaac tagacgaagg ctaaacttgt cagtgatatt gattttagcc
     4981 cctgtaagtt ccttgttaat gatgattttg agttggaagc cttcaccgct gtttggcact
     5041 ttttccaaaa ggcgagtcag ttcatagtta ccaaccttag tttcaaaaaa ggtgttatct
     5101 ttgagggtga attttttaac agaagggcta agagtgtaat cgtaacgaca attttttaac
     5161 tgaatgattt tttcaaatgc catatggcta acctccgata atttctttta aggtttttgc
     5221 gagggtttgt aggtcttcaa cggtattttg tggcgacaaa ctgatgcgaa gggattcctt
     5281 caagcgttct gaatttgcgc catacatggc ttcaagaaca tggctggatt ggacaacgcc
     5341 tgcagtacag gctgagccag tagagattga aattccagct aaatctagcc gaaggagtaa
     5401 gaggtcattt ttctgaccag gaaatccaat attgagaaca taagggagat gatgttttcc
     5461 tctattcaag taatactgaa tgccctccag ctctgccaga aaggcggttt ctagattttg
     5521 tacatgttga aaatgttctt cttgtttttc taggtcttct tttagggctg caaccatgcc
     5581 tacgatagca ggcagatttt cagttcctgc acgttttttc tgttcctggt ctccgccatg
     5641 tagataggaa tcaaagtcca tgctagatgc gtagagaaaa ccgattccct taggaccatg
     5701 gaatttgtgg gcagaagcag tgagaaaatc aatgcccaat tcttctgaat gaattgggat
     5761 tttaccaata gcctgaactg catcaacatg ataggcagca gggtgttgct tgagtatttg
     5821 gccaatttca gcgatgggca gtaggtttcc tgtctcatta ttggcaaaca tggtagaaac
     5881 caaaatcgta tcgtcacgta aagccttttg aatttgctgg gctgtgattt cttgattttc
     5941 tggctggata atggttgctt caaacccaaa gtgttgaacc aagtaatcaa ttgtttcaag
     6001 gacagcatgg tgctcgatgg cagttgtgat gatatgtttt ccttgttctt ggtgacgaag
     6061 acagtagcca atgatggtag tattattgcc ttcagtccca ccagaagtga aaaagatatg
     6121 ttgaggtttt gtccttagta actgggctag ttcctgacgg gcttctcgca agagtttgcc
     6181 agcttgacga ccatgaccat gaatactaga aggatttccg tgggtttctt gcataacctt
     6241 ggtcatagct gaaatagcaa ctgctgacat aggagtcgtt gcagcattgt ccaaataaat
     6301 caaagaatca ccttatttct ttttattgta ggcaaagagt gggctgactg gttttctttc
     6361 gtgaatacgg acgatagcat caccaattaa ctcactagca gtgatgtagc atacattttt
     6421 aggagttttt tcttttgttg ctactgaatc agtcacaaga atttctttaa tattagtatt
     6481 gtcaagaagc tcagcagctc cctcgacgaa gagaccgtgg ctagaaacag cataaatttc
     6541 tgtagctcct tcacgttcaa cgattttaga agcttcagag aaggtacgtc ctgtatttaa
     6601 aatatcatca atcaagatag ctttcttacc ttcaacatca ccaataatat aaccttcgtt
     6661 acgagttgca tcgtcttgag ggtagtcgat aatggcgata ggagcatcaa gatattcagc
     6721 caggctacgc gcacgtttga cacctgaatt tttagggcta acgacaacaa catctgaacc
     6781 aagcaatcct ttatcgcagt aatgttttgc gaatagggga acagtgaaaa gattatccac
     6841 tggaatatca aagaaacctt gaacctgaac ggcatgcaaa tcaagagtca ggatacgatc
     6901 aactccagcc ttaaccagca tattggcaac tagttttgct gtaagtggct cacgaggaca
     6961 agcaatgcgg tcttgacgtg catagccaaa atatggaagg acaacgttga tactgtgggc
     7021 acttgcacgc acacaagcat cgaccatgat taacaattcc attaggtggt tgttgacagg
     7081 gaaacttgtt gattggatga tgtaaacatc ataaccacgg acactttctt cgatatttac
     7141 ttggatttct ccgtctgaaa attgacgtga tgatagtttt ccaagtggga caccaacagc
     7201 ttgggcaatt ttttgtgcaa tctcttggtt agagttgagt gcgaaaagtt tcatgttttt
     7261 tctatctgac attatagacc gtcctctgta aactttataa atcctagtta tatttacctt
     7321 acatatatga actgggattt gtgtattttt atcttttcta ttttaccaaa aaatggagat
     7381 tatttcagct atttttcata cttttgacaa atcgaaccaa ttttgaagga gctttttggt
     7441 aggaaatctg atttttctct aaaaattgtc ggaaatcctg tttgccttgc tcatgatttt
     7501 ccacttcaag ctccaattcg taatctgtta tatcaaagta tcggctctga tccagtgcca
     7561 tgagaccaat agctgttttc atttcatagc gaagcgttgt tagacaacca agaacctgcc
     7621 agttcttact ttggatacca tgtttcgcca attcatccag tactagtcct tgaggaagtt
     7681 cttccttact cagatagtcc tcagcatctt ttagttgcaa tttttggttg tattccatgt
     7741 ttccaacact ctgcgggact ttgagtgtca actcagccca gtcttcaaag gttcgaatgc
     7801 gcatagcgac tttcttttct cgcagttcaa aatcaggcgt gtcgatgtag taatttgttt
     7861 gaagaacagg agtgacacct gtgaactggt cttttagacg attgtattca tcttttttca
     7921 atagtgtttt caattcaatt tctaaatgtt tcatttttct tacctttttt tatcgttgaa
     7981 agcggattta tggtataata agcattgtat ttattgtata tgaatctgga gaaaaaatca
     8041 aagatatttt tgacggataa tatgagaaca agggagaata tatgacctta gaatgggaag
     8101 aatttctaga tccttacatt caagctgttg gtgagttaaa gattaaactt cgtggtattc
     8161 gtaagcaata tcgtaagcaa aataagcatt ctccaattga gtttgtgacc ggtcgagtca
     8221 agccaattga gagcatcaaa gaaaaaatgg ctcgtcgtgg cattacttat gcgaccttgg
     8281 aacacgattt gcaggatatt gctggcttac gtgtgatggt tcagtttgta gatgacgtca
     8341 aggaagtagt ggatattttg cacaagcgtc aggatatgcg aatcatacag gagcgagatt
     8401 acattactca tagaaaagca tcaggctatc gttcctatca tgtggtagta gaatatacgg
     8461 ttgataccat caatggagct aagactattt tggcagaaat tcaaattcgt actttggcca
     8521 tgaatttctg ggcaacgata gaacattctc tcaactacaa gtaccaaggg gatttcccag
     8581 atgagattaa gaagcgactg gaaattacag ctagaatcgc ccatcagttg gatgaagaaa
     8641 tgggtgaaat tcgtgatgat atccaagaag cccaggcact ttttgatcct ttgagtagaa
     8701 aattaaatga cggtgtagga aacagtgacg atacagatga agaatacagg taaacgaatt
     8761 gatctgatag ccaatagaaa accgcagagt caaagggttt tgtatgaatt gcgagatcgt
     8821 ttgaagagaa atcagtttat actcaatgat accaatccgg atattgtcat ttccattggc
     8881 ggggatggta tgctcttgtc ggcctttcat aagtacgaaa atcagcttga caaggtccgc
     8941 tttatcggtc ttcatactgg acatttgggc ttctatacag attatcgtga ttttgagttg
     9001 gacaagctag tgactaattt gcagctagat actggggcaa gggtttctta ccctgttctg
     9061 aatgtgaagg tctttcttga aaatggtgaa gttaagattt tcagagcact caacgaagcc
     9121 agcatccgca ggtctgatcg aaccatggtg gcagatattg taataaatgg tgttcccttt
     9181 gaacgttttc gtggagacgg gctaacagtt tcgacaccga ctggtagtac tgcctataac
     9241 aagtctcttg gcggtgctgt tttacaccct accattgaag ctttgcaatt aacggaaatt
     9301 gccagcctta ataatcgtgt ttatcgaaca ctgggctctt ccattattgt gcctaagaag
     9361 gataagattg aacttattcc aacaagaaac gattatcata ctatttcggt tgacaatagc
     9421 gtttattctt tccgtaatat tgagcgtatt gagtatcaaa tcgaccatca taagattcac
     9481 tttgtcgcga ctcctagcca taccagtttc tggaaccgtg ttaaggatgc ctttatcggt
     9541 gaggtggatg aatgaggttt gaatttatcg cagatgaaca tgtcaaggtt aagacctttt
     9601 taaaaaagca cgaggtttct aagggattgc tggctaagat taagtttcga ggtggagcta
     9661 ttctggtcaa taatcaaccg caaaatgcaa cgtatctatt ggacgttgga gactacgtta
     9721 ccattgacat tcccgctgag gaaggctttg aaaccttgga ggctattgag catccattag
     9781 atattctcta tgaggatgac cactttctag tcttgaataa accctatgga gtggcttcta
     9841 ttcctagtgt caatcactct aacaccattg ccaattttat caagggttac tatgtcaagc
     9901 aaaattatga aaatcagcag gttcacattg ttaccagact agatagggac acttctggct
     9961 tgatgctctt tgccaagcac ggttatgccc atgcacgatt agacaagcag ttgcagaaga
    10021 aatctatcga gaaacgctac tttgctttgg ttaagggaga tggacatttg gagccagaag
    10081 gggaaattat tgctccgatt gcgcgtgatg aagattccat tattaccaga cgagtggcta
    10141 aaggcggaaa gtatgcccat acttcataca agattgtagc ttcttatgga aatattcact
    10201 tggtctatat tcacctgcac actggtcgaa cccatcaaat ccgagtccat ttttctcata
    10261 tcggttttcc tttgctggga gatgatttgt atggtggtag tctggaagat ggtattcaac
    10321 gtcaggctct gcattgccat tacctatcct tttatcatcc atttttagag caagacttgc
    10381 agttagaaag tcccttgccg gatgatttca gtaaccttat tacccagtta tcaactaata
    10441 ctctataaaa actgtctcag agtataatta ttatcttaaa ggagaaaact catggaagtt
    10501 tttgaaagtc tcaaagccaa ccttgttggt aaaaatgctc gtatcgttct ccctgaaggg
    10561 gaagagcctc gtattcttca agcaacaaaa cgcttagtaa aagaaacaga agtgattcct
    10621 gttttgcttg gaaatcctga aaaaattaaa atttatcttg aaattgaagg aatcatggat
    10681 ggttatgagg tcatcgaccc tcaacattat cctcaatttg aagaaatggt ttctgccttg
    10741 gtggagcgtc gcaagggcaa aatgactgaa gaagatgtac gcaaggtttt ggttgaagat
    10801 gtcaactact ttggtgtgat gttggtttac ttgggcttgg ttgatggaat ggtgtcagga
    10861 gcgattcact caacagcttc aacagttcgt ccagctctac aaatcatcaa aactcgtcca
    10921 aatgtaactc gtacttcagg agccttcctc atggttcgtg gtacggaacg ttacctattt
    10981 ggagactgtg ccattaacat caatccagat gcagaagcct tggctgaaat tgccatcaac
    11041 tcagcaatca cagctaagat gtttggcatc gaacctaaaa ttgccatgtt gagctattct
    11101 actaaaggtt cagggtttgg tgaaagcgtt gataaggtcg ttgaagcaac taaaattgct
    11161 cacgacttgc gtcctgacct tgaaatcgat ggtgagttgc aatttgatgc agcctttgtt
    11221 cctgaaactg cagctctgaa agctcctgga agtacggtag ctggtcaagc aaatgtcttc
    11281 atcttcccag gtatcgaggc cggaaatatc ggttacaaga tggctgaacg cctgggtggc
    11341 tttgcggctg taggacctgt tttgcaaggt ttaaacaagc cagttaatga tctttctcgt
    11401 ggatgtaatg cagatgatgt ttacaagttg accctcatca cagcagctca agcagttcat
    11461 caatagtgaa aactataaag cgatatacta tgctatactg tagttatgaa actatgtacg
    11521 aaaagcactg ccattaattc ctgagaacta aattactgat tggtgtcaaa aaggaaaact
    11581 tccaagcgat gatatcctgt ctatacacga cctatagaaa tctgtaatat gtatgtccgt
    11641 aaaacgataa tttccctttt tgattttaaa tgagtatgaa aagagaattt tttcatgaga
    11701 aaacgcataa tattaagtgt cttgacacct gctattatgc gtttt
//
LOCUS       DAVBHS010000056        11547 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVBHS010000056 DAVBHS010000000
VERSION     DAVBHS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11547
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.056"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            395..1105
                     /gene="deoD"
                     /locus_tag="VQ651_001079"
     CDS             395..1105
                     /gene="deoD"
                     /locus_tag="VQ651_001079"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET2754087.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1128..1652)
                     /locus_tag="VQ651_001080"
     CDS             complement(1128..1652)
                     /locus_tag="VQ651_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HET2754088.1"
                     /translation="MKIAIIGYSGSGKSTIAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1705..1941)
                     /locus_tag="VQ651_001081"
     CDS             complement(1705..1941)
                     /locus_tag="VQ651_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET2754089.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(2009..2929)
                     /locus_tag="VQ651_001082"
     CDS             complement(2009..2929)
                     /locus_tag="VQ651_001082"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HET2754090.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            3119..3523
                     /locus_tag="VQ651_001083"
     CDS             3119..3523
                     /locus_tag="VQ651_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754091.1"
                     /translation="MKILKRYILELCFILSFALPFIKGTNADNGRCFVETYYGFTFLM
                     EHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            3739..4320
                     /locus_tag="VQ651_001084"
     CDS             3739..4320
                     /locus_tag="VQ651_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2754092.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGQKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            4317..5594
                     /locus_tag="VQ651_001085"
     CDS             4317..5594
                     /locus_tag="VQ651_001085"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HET2754093.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIRGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEAREFA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVHQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            5612..6274
                     /gene="deoC"
                     /locus_tag="VQ651_001086"
     CDS             5612..6274
                     /gene="deoC"
                     /locus_tag="VQ651_001086"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET2754094.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            6261..6650
                     /locus_tag="VQ651_001087"
     CDS             6261..6650
                     /locus_tag="VQ651_001087"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2754095.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6740..7792
                     /locus_tag="VQ651_001088"
     CDS             6740..7792
                     /locus_tag="VQ651_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HET2754096.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALNNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            7936..9471
                     /locus_tag="VQ651_001089"
     CDS             7936..9471
                     /locus_tag="VQ651_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754097.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            9464..10522
                     /locus_tag="VQ651_001090"
     CDS             9464..10522
                     /locus_tag="VQ651_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2754098.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            10525..11481
                     /locus_tag="VQ651_001091"
     CDS             10525..11481
                     /locus_tag="VQ651_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2754099.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         3283 a         2065 c         2642 g         3557 t
ORIGIN      
        1 agaaaggtag agcgcgtgtt ctaatttgaa cacgagtaga aaacttttct aaaaacaaaa
       61 acgaaaggat gggtaaactg tattcgctga actgaatacg ggcgactctc ctctaaatca
      121 aaattaagaa aggaattgac cccaccctaa aagtagtggg aaaaagatag ttggtctagc
      181 gagcatcgct cactgcgccc aactcctatt ttcccttcgc tttttgatgg gtttggtatc
      241 tttctcaata taaaataaag aaaggtagag cgtgtgtttt gatttgaaca cgagcggaaa
      301 actcggaaaa tagataatct gactgaaaaa tcaggatttc tcgtcaggtt cctaattttc
      361 agtcgttttc ttctcgctct ttgtatcata aattatgtct atccatattg ctgctcagca
      421 gggtgaaatt gctgataaaa ttcttcttcc tggggatcct cttcgtgcta agtttattgc
      481 ggagaatttc cttgatgatg ctgtttgttt taacgaagtg cgtaacatgt ttggttacac
      541 tggtacttac aagggtcact gtgtatctgt catgggaact gggatgggaa tgccatctat
      601 ttcgatttat gcgcgtgagt taatcgtaga ctacggtgtg aagaaattga ttcgtgtggg
      661 aactgcaggt tctttgaatg aagaggttca tgttcgtgaa ttagttttgg cgcaggcggc
      721 tgcaaccaac tcaaacatcg ttcgtaatga ctggccacag tacgattttc cacaaattgc
      781 tagctttgat ttgcttgata aagcctacca tatcgccaaa aaacttggta tgactactca
      841 cgttgggaac gttttgtcat ctgatgtctt ttactcaaat tactttgaaa agaatatcga
      901 gcttggtaaa tggggagtca aggctgtgga aatggaagca gcagctcttt actatcttgc
      961 tgcccaatac catgttgatg cgctagctat catgaccatc tctgatagct tggtcaatcc
     1021 agacgaagac acaactgcag aagaacgtca aaataccttc actgatatga tgaaggttgg
     1081 tttggaaacc ttgattgcag aataattata gccaaaaagg aagcccttta tgaattgtag
     1141 gacttccttt tcttatccag aaattgatct agctctctct gatttcgaag aatagtgact
     1201 ttatgtgaat attcttggca aagtttttgg taattttctt tttgagtttt gctacgccca
     1261 tcccaaagaa tccatctgat aaactcccac tcaaagcgtt cagggcaatc tgccgccata
     1321 ctttctctga cttttccacg gtatttaaga taacgcttaa aggctctaaa gagacaggtc
     1381 aatggcgaaa aattgagaaa gatgatttgg tcagcttctt gcattcgttc ttggtagtag
     1441 caccaagaat aattaccatc gatgacccaa gctttatgct tggtgagaaa gttttttatc
     1501 tcggttaaca tccattcgca gtcactgtct tgccaaccag gttgaaattg gagtgtgtcc
     1561 atgtgcagtt ttggaatgga gtagtagtta gataactttt ctgctatagt tgacttacca
     1621 gaaccagaat atccgataat tgcgattttc attttctact ttttcctatt tggagacaaa
     1681 aaaacagcct ctatggactg tttcttattt agcaagttta gctgaaagac gagctttatc
     1741 gcggcttgct ttgtttttgt gaatcaaacc tttagtttct gctttatcga tagctgagct
     1801 agcagcacgg aaaagttctt cagatgggtt tgcttcgaaa gcttttatag cagtacgcat
     1861 agctgatttt tgagctgagt tcttttcgtt ttgtttaacg ttcaattcag cgcgtttgat
     1921 agctgattta atgtttgcca atgttcttac ctccatattt actaactata ccattatata
     1981 tgaaaactta cgttttgaca agggaaaatt acttttttaa gtaaatttca tcgatttcat
     2041 ggttcttgct tttatgaaga atcacttctg cacgatttct ggttggttca atataatttt
     2101 gcagatttgt gagattgata ctgatccaga cctgatgggc aaaggactcc acttccccaa
     2161 tcggcatctg agtaaaacga taatagtagc tatcagggtc gttttgggct aggctcagca
     2221 ttttcaagaa acggtccaga taccaacttt caatatcatc cactccagca tctacataga
     2281 tggaaaagtc aaagaagtca gtgatataga gacgatcgtt ttgtggattt tgaaagacat
     2341 taattccctc aacaattaca aaatcagcag ctttgacact ttgtttcttt tcgggtacga
     2401 tgtcgtaaac ttcatgagaa tagacaggaa tatctacatc ttgtccattt ttgatgcggt
     2461 ccaagaagtt gagaagagct tccatatcat agctttcagg aaatccttta cgatttaaaa
     2521 tcccctgctc aatcaaggtt tgattgggat agagaaaacc atcagttgta accaactcaa
     2581 ccgtagcatc tgtaaacgta cgggacagta ggatttgaag taggcgactg gttgtggatt
     2641 ttccaacggc aacactccca gaaaccccaa taataaaagg ttgagattta ctttcacgtt
     2701 ggaggaaaat tccttttgaa aaggctaaat cttccttagt tcgcttgtaa atctgaatca
     2761 gatgagccaa ggggagatag atatctgtaa cgtcttggag actgatttgg tcattaaaac
     2821 tcttgataga ttccaattct tcttctgtca aaggaggtgt tgtctttcga tgtaaagatt
     2881 gccaagtctg gcggctgatt ttttcaaaat gtaaaaattc gttggtcatt tgttttcccc
     2941 tagatatttt gtctatcata ccataaaaag ctaaaaaaat aaagcggttt cttgaatgtg
     3001 gtaagtcaat ggtatatagt ataagtgatc aatttattga tgaaatagga gatagattct
     3061 actgatttat aagcttataa agggggcttt ttagttcaca acttatagga ggtgtactat
     3121 gaaaatctta aaacgttaca tattggaact ctgttttatt ttaagttttg ctttaccttt
     3181 tataaaagga accaatgcag ataatggtag atgctttgtg gaaacctatt acggttttac
     3241 ttttttgatg gaacatgcta ttgtaacagc tgtctttatc tattcgtttt taattgcttt
     3301 cttactaaaa aaacgatggg cgaaatggat tgctgctggt agttattgct ttttagtcct
     3361 atggattgcg acagagggtt atttcttccg catgtcacta gaagatttga tacgactttg
     3421 gacaagtttg gaaatcctga cacaaacgta tcagttgggc ttttatctaa acattttgtt
     3481 aggaattttg ttgataataa agtattttaa ggttaagcaa tagaaataat aatctgcctg
     3541 attatagatg ataaactttt tgtttttatc tggactgtga tatttatagt tctaaatggt
     3601 ttattattta taagtgttga aagagattga agaaaatggc ttcgtgactt gctttcttcg
     3661 gtctttttcg ttttattatc aatttgtaaa cgatttacaa tttgagtcaa attatggtaa
     3721 aatagtgtca tgagtaaaat gtattatgca gaaaatcctg acgctgctca cgacattcat
     3781 gagttgagag tggacttgtt gggacaaaaa atgacttttt tgacagatgc gggtgttttt
     3841 agcaagaaaa tggttgactt tggaagtcaa ctcttgctca agtgtctgga tgtcaaccaa
     3901 ggagagacag tcctagatgt aggctgtggt tatggtccat taggtttgtc cttggtcaag
     3961 gtttacggag ttcaggcgac catggttgat attaacactc gtgccttgga tttagctcgg
     4021 agaaatgctg aaaaaaataa tgcaaaagcg acgattttcc agtccaacat ctatgaacaa
     4081 gttgaaggac attttgatca tgtcatttcc aatcctccta tccgtgcggg caagcaagtc
     4141 gttcatgaaa tcattgagaa aagtaaagat ttcttggaaa ctggtgggga tttgacaatc
     4201 gtcatccaga aaaaacaagg ggctccaagt gctaagtcca agatggagga tgtgtttggt
     4261 aattgtgaaa tccttaaaaa agacaaagga tactatatcc ttagaagtgt gaaagaatga
     4321 gagcagttga tttaatacaa aaaaaacgag acggtcaaga actgacttca agtgaaattg
     4381 aatggctggt agaaggctat gtgtcaggaa ctgttcctga ttatcagatg tctgcctttg
     4441 ctatggctgt ttattttaaa ggaatgacga ctcgtgaaat ttctgattta acgatgaata
     4501 tggttaagac tgggcaagag tttgacctgt cagccattga tggtgtgaaa gttgacaagc
     4561 attctactgg tggtgtcggc gataaggtaa ccttgatttt agctcctctt gttgctagct
     4621 tcggtgtgcc tgtagcaaaa atgagtggtc gtggtcttgg ccatacaggc ggaacaattg
     4681 ataaattgga gtccattagg ggctatcaag tagaacgtag tcaagaggat tttattcgtc
     4741 aggttcagga tattggagta tctgtcattg ggcaatcaga ccagctggtc aaagcagata
     4801 agcttctcta tgcccttcgt gatgtgaccg caactgtcga cacgattcct ttgattgcga
     4861 gttcggtgat gagcaagaaa attgcggcag gggcggatgc tattttgcta gacgtgactg
     4921 tcggtgaggg tgccttcatg aagacggttg atgaggcgcg tgagtttgct caaactatgg
     4981 tagatcttgg taaggttgtt ggacggaaga cagtagcagt gattacggat atgagccagc
     5041 ctttgggacg agcgattgga aatcgtctgg aaatacttga agcattggag attttgcaag
     5101 gtcaaggacg tcaggatatt acccacttta tttgtgaatt ggctcaaatt atgcttggtt
     5161 tggcaaatgt taacaagact gttgaagaag ttcaccaaca tcttgagaat ggtcaagcac
     5221 tggctaagtt tgaggaaatg gtgcaagccc aaggtggaga cttggaagac ctctatcgtc
     5281 ctgtaaatgt agctcatgtg gtggaaatcc ctgctcagga aacgggtgtc atttcagctc
     5341 ttccagctat ggactttggt ctttatgcca tgaggctggg ggctggtcgt gcagttaagt
     5401 ctgatgcctt ggactatgaa acaggaattg tttttgaaaa gaagattgga gactccgttc
     5461 aaaagggtga aattgttgca aaagtataca caaatgaaaa aattccacct caactagtta
     5521 cagatttcca aaaatgtgtt aaaataagtg atgaagtgaa aaaaatccgt gaaattgtag
     5581 aaatcatctc ctaaatggag ggaaaaccga aatgaaatta aataaatata tagatcatac
     5641 gcttttaaaa caagatgcaa agaaaaaaca aattgatagt ttgttgtctg aggctagaga
     5701 gtatgacttt gccagtgttt gcgttaatcc gacctgggtt gaacatgcta aaaaaggact
     5761 tgaaggcaca gatgttaagg tttgcacggt agtaggtttc cctttgggag caacaacttc
     5821 agccgtgaaa gcatttgaga caaaagaagc tatccaaaat ggtgcagatg agattgatat
     5881 ggtgatcaat gttggagctc tcaaatcagg taatttagcc ttggttgagt cagatattcg
     5941 cgcagtagtg gaagcaagtg gtgataagtt agtgaaagtc attattgaag cttgccttct
     6001 gacagaccaa gaaaaaattg ttgtttgcca attggcccaa aaagctgggg ctgactttgt
     6061 taaaacatct actggttttt caactggtgg tgctacgata gcagatgtta cattaatgcg
     6121 tgaaacagtt ggatctgata tgggagtcaa ggctgctggt ggagctcgtt cttatgcaga
     6181 tgctcttgcc tttgtcgaag caggtgcgac ccgtatcgga acgtcagctg gggtagctat
     6241 tttaaaagga gaattggcag atggcgacta ctgagttgat tgaactggca attgaaacca
     6301 gcaaacatgc ctatgtcccc tattctcact ttcctatcgg agcagtttta gtagccaaag
     6361 acggaagtgt ttacacggga gtgaatatcg aaaatgctag ctatcctttg accaattgcg
     6421 gtgagagaac agctattttt aaggctatat ctgaaggcca aagagaattt tcagaattga
     6481 ttgtctatgg tcaaactgaa aaaccgatct cgccatgtgg tgcttgtcgc caagtgatgg
     6541 ctgaattttt tgaacaagat ctaaaagtga ccttagtcgc aaaagataaa tcgacggtcg
     6601 agatgacggt cggggaatta cttccatact cttttacaga cttaaactag tctgagtcgc
     6661 tctctgagtg gcacggtcct tgtggccaac caatccatac ttgcaacatc gttgcacatc
     6721 ttatttagga ggttcagtaa tgaacaagaa acaatggcta ggtcttggcc tagttgcagt
     6781 ggcagcagtt ggacttgctg catgtggtaa ccgctcttct cgtaacgcag cttcatcttc
     6841 tgatgtgaag acaaaagcag caatcgtcac tgatactggt ggtgttgatg acaaatcatt
     6901 caaccaatca gcttgggaag gtttgcaggc ttggggtaaa gaacacaatc tttcaaaaga
     6961 taacggtttc acttacttcc aatcaacaag tgaagctgac tacgctaaca acttgcaaca
     7021 agcggctgga agttacaacc taatcttcgg tgttggtttt gcccttaata atgcagttaa
     7081 agatgcagca aaagaacaca ctgacttgaa ctatgtcttg attgatgatg tgattaaaga
     7141 ccaaaagaat gttgcgagcg taactttcgc tgataatgag tcaggttacc ttgcaggtgt
     7201 ggctgcagca aaaacaacta agacaaaaca agttggtttt gtaggtggta tcgaatctga
     7261 agttatctct cgttttgaag caggattcaa ggctggtgtt gcgtcagtag acccatctat
     7321 caaagtccaa gttgactacg ctggttcatt tggtgatgcg gctaaaggta aaacaattgc
     7381 agccgcacaa tacgcagccg gtgcagatat tgtttaccaa gtagctggtg gtacaggtgc
     7441 aggtgtcttt gcagaggcaa aatctctcaa cgaaagccgt cctgaaaatg aaaaagtttg
     7501 ggttatcggt gttgatcgtg accaagaagc agaaggtaaa tacacttcta aagatggcaa
     7561 agaatcaaac tttgttcttg tatctacttt gaaacaagtt ggtacaactg taaaagatat
     7621 ttctaacaag gcagaaaaag gagaattccc tggcggtcaa gtgatcgttt actcattgaa
     7681 ggataaaggg gttgacttgg cagtaacaaa cctttcagaa gaaggtaaaa aagctgtcga
     7741 agatgcaaaa gctaaaatcc ttgatggaag cgtaaaagtt cctgaaaaat aatggatgga
     7801 agtcatttct taggaagcgg ccaagggccg cttttttaag agttgagaca aagtcatttt
     7861 gtctcagtaa agcttgctac tagacaaact agcaagtttt attgaaataa aataagactc
     7921 tgaaaggaag agcacatggc acacgaaaat gtcattgaga tgcgtgatat taccaaggtg
     7981 tttggtggat ttgttgccaa cgacaaaatc aacttgcacc tacgaaaagg tgaaattcat
     8041 gcacttttag gagaaaatgg ggctggtaag tccacgctaa tgaacatgtt agcaggcctt
     8101 cttgaaccaa ctagtggtga aatcgcggtc aacggtcaag ttgtcaatct cgactcccca
     8161 tctaaagcag ctagcttggg aatcgggatg gttcaccagc actttatgtt ggttgaagcc
     8221 ttcacagtgg ctgaaaacat cattttaggt agtgaattga ctaaaaatgg tgtgctagat
     8281 atcgctggag ctagcaaaga aatcaaggct ctttctgaac gttatggctt agctgttgac
     8341 ccttctgcca aggtagcaga tatctcagtt ggagcccaac aacgtgtaga aattttaaaa
     8401 acactttatc ggggggctga tatccttatc tttgacgaac caacggctgt tttgactcca
     8461 tcagaaattg atgagttgat ggctattatg aaaaatcttg tcaaagaagg aaaatcaatt
     8521 atcttgatta cccacaaatt ggatgaaatt cgcgcagttt ctgaccgtgt tacagttatc
     8581 cgtcgtggga aatcaattga aaccgttgaa attgcagggg ctaccaatgc tgatttggcg
     8641 gaaatgatgg taggacgttc tgtttccttt aaaacagaga agcaagcctc taaaccaaaa
     8701 gaagtggttt tgtctatcaa agatttggtg gtcaatgaaa accgtggtgt tccagctgtt
     8761 aaaaatctgt ccttggatgt tcgtgctgga gagattgttg gtattgcggg gattgatgga
     8821 aatggtcagt ctgaactgat tcaagccatt acaggtcttc gtaaggttga atctggtagc
     8881 attgagctaa aaggagattc aattgtaggc ttgcacccac gtcagattac agaactaagt
     8941 gttgggcacg ttccagaaga ccgtcaccgt gatggcttga ttttggaaat gatgatatct
     9001 gaaaatattg cccttcaaac ctactataaa gaaccacata gtaaaaatgg aattttgaat
     9061 tattcaaata ttacttctta tgctaaaaag ctgatggaag agtttgatgt tcgcgctgcc
     9121 agtgaattag ttcctgcagc tgcactctca ggaggaaatc aacaaaaagc aattattgct
     9181 cgtgaaattg atcgagatcc tgatctcctt atcgttagcc agccaactcg tgggttggat
     9241 gtcggtgcca ttgagtatat ccacaaacgc ttgattgaag agcgtgataa tggcaaggct
     9301 gtccttgttg tcagctttga attggatgag attttaaacg tctcagaccg tattgccgtt
     9361 atccacgatg gtaagattca aggtattgta tcaccagaaa caaccaataa acaagaactt
     9421 ggtgtcttga tggctggtgg aaacttggga aaggagaaga gtgatgtcta aaaaattaca
     9481 acaaatttcg gttcccttga tttctgtatt tctaggaatt ttactcggag ccattgtcat
     9541 gtggatcttc ggttatgatg ctatttgggg ctacgaagaa ttgttctata cagcctttgg
     9601 cagtctgcgt gggattggag aaatcttccg tgctatgggt cctctggtct tgattggtct
     9661 tggttttgcg gttgccagtc gagctggttt ctttaacgtc ggacttcctg gtcaggcttt
     9721 ggcaggttgg attctcagtg gttggtttgc gctgtcgcat ccagatatgc cccgtccctt
     9781 gatgattcta gcaaccatcg tgattgcctt gattgctggt gggattgtcg gagcgattcc
     9841 aggtattctt agggcctatc tagggacgtc agaggttatt gtaaccatca tgatgaacta
     9901 cattgtcttg tatgtaggga atgcctttat ccatgctttc cctaaagact tcatgcaaag
     9961 tacagattcg accattcgtg ttggggctaa tgcaacctat cagacacctt ggttggctga
    10021 gttgactggt aactcacgga tgaatattgg tattttcttt gccatcattg ccgttgcagt
    10081 tatttggttc atgctcaaga aaacaactct tggttttgaa atccgtgcag ttggtcttaa
    10141 tccacatgct tcagaatatg ctggtatttc tgccaagcgg actattatcc tatctatgat
    10201 tatttcaggt gccttggcag gtcttggtgg agctgttgaa ggtttgggaa ccttccagaa
    10261 cgtctatgtt caaggttcgt cattagctat cggatttaac ggaatggcgg ttagtttgct
    10321 tgcggccaac tcaccaattg gtatactctt tgcagccttc ctatttggcg ttctccaagt
    10381 tggggctcct ggtatgaatg cggcgcaggt accatctgag cttgtcagca ttgtaacagc
    10441 gtctattatc ttctttgtca gtgttcatta ccttatcgaa cgctttgtca aaccgaaaaa
    10501 acaagttaaa ggaggtaagt aaagatgtct attataacct tgctccccct cttggtgtct
    10561 tctatgctga tttactcagc acctctcatc tttacaagta tcggtggtgt tttctctgaa
    10621 cgtggtggtg tggtaaacgt tggccttgaa ggaattatgg ttatgggtgc cttttctgga
    10681 gttgtcttta accttgaatt tgcagaacaa tttggagcag caactccatg gctatccttg
    10741 cttgtagcag gattggttgg tagtgttttc tctatcatcc acgcagcagc gacggttcat
    10801 ttccgtgcag accatgttgt cagcggtacg gtattgaact tgatggcgcc tgccttggct
    10861 gttttcttag ttaaagttct ttataacaaa ggacaaaccg acaacctaag tcaaactttt
    10921 ggacgctttg atttcccagt cttggcaaat atcccagtga ttggtgatat cttcttcaag
    10981 tcaactagtc tacttggtta tctggcgatt gccttctcat tccttgcttg gtttattctc
    11041 tttaagactc aatttggtct tcgtctccgc tctgtcggtg aacatcctca agcagcggat
    11101 accttgggaa tcaacgtcta caagatgaga tatttaggga ttattatttc aggttttcta
    11161 ggtggaattg gcggagcgat ttatgcgcaa tcaatctcag ttaacttctc agtgacaact
    11221 attgttggac ctggatttat cgcccttgct gcgatgatct ttgggaaatg gaatccaatc
    11281 ggtgctatgc tttctagtct cttctttgga ctttcacaaa gtttggctgt tatcggttct
    11341 caattgccgt tcctacaagg agtgccagcg gtttatcttc aaattgcacc ttatgttttg
    11401 acaattcttg tcttagcagc cttctttgga aaagcagtcg caccaaaagc agatggtatc
    11461 aactacatca aatcaaaata agcatacaaa aaaacgtcag tccaatgact gaactgcccc
    11521 ccaaaagtta gacagaaaaa atctaac
//
LOCUS       DAVBHS010000057        11469 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVBHS010000057 DAVBHS010000000
VERSION     DAVBHS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11469
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.057"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            125..379
                     /gene="blpM"
                     /locus_tag="VQ651_001092"
     CDS             125..379
                     /gene="blpM"
                     /locus_tag="VQ651_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HET2754100.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            395..598
                     /gene="blpN"
                     /locus_tag="VQ651_001093"
     CDS             395..598
                     /gene="blpN"
                     /locus_tag="VQ651_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HET2754101.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            842..991
                     /gene="blpO"
                     /locus_tag="VQ651_001094"
     CDS             842..991
                     /gene="blpO"
                     /locus_tag="VQ651_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HET2754102.1"
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            1095..1214
                     /locus_tag="VQ651_001095"
     CDS             1095..1214
                     /locus_tag="VQ651_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HET2754103.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            2000..2398
                     /locus_tag="VQ651_001096"
     CDS             2000..2398
                     /locus_tag="VQ651_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET2754104.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEVGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKKQTVFLILQQ"
     gene            2436..3125
                     /locus_tag="VQ651_001097"
     CDS             2436..3125
                     /locus_tag="VQ651_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2754105.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNFV
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            3167..3415
                     /locus_tag="VQ651_001098"
     CDS             3167..3415
                     /locus_tag="VQ651_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754106.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLKWFVLERLELKDDA"
     gene            3445..4056
                     /locus_tag="VQ651_001099"
     CDS             3445..4056
                     /locus_tag="VQ651_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2754107.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            4217..5011
                     /locus_tag="VQ651_001100"
     CDS             4217..5011
                     /locus_tag="VQ651_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET2754108.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            5008..5643
                     /gene="trmB"
                     /locus_tag="VQ651_001101"
     CDS             5008..5643
                     /gene="trmB"
                     /locus_tag="VQ651_001101"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET2754109.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            5769..6248
                     /gene="rimP"
                     /locus_tag="VQ651_001102"
     CDS             5769..6248
                     /gene="rimP"
                     /locus_tag="VQ651_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET2754110.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            6292..7428
                     /gene="nusA"
                     /locus_tag="VQ651_001103"
     CDS             6292..7428
                     /gene="nusA"
                     /locus_tag="VQ651_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HET2754111.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            7450..7743
                     /locus_tag="VQ651_001104"
     CDS             7450..7743
                     /locus_tag="VQ651_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HET2754112.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            7736..8035
                     /locus_tag="VQ651_001105"
     CDS             7736..8035
                     /locus_tag="VQ651_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HET2754113.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            8052..10844
                     /gene="infB"
                     /locus_tag="VQ651_001106"
     CDS             8052..10844
                     /gene="infB"
                     /locus_tag="VQ651_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET2754114.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            11095..11445
                     /gene="rbfA"
                     /locus_tag="VQ651_001107"
     CDS             11095..11445
                     /gene="rbfA"
                     /locus_tag="VQ651_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET2754115.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3505 a         2010 c         2610 g         3344 t
ORIGIN      
        1 ttgcaattca agacgtttcg atgactattc aagatctgga tgaaaaattt taaaaaacga
       61 tgatatacta aacttgtcaa agttacaaca agacaaaaat taaaaataaa aaagagtatt
      121 tgtcatggat acaaaaatta tggaacaatt tcatgaaatg gatataacta tgttatctag
      181 tattgaagga ggcaagaata attggcaaac taatgtcttg gaaggtggtg gtgctgcttt
      241 tggtggctgg gggctaggga cagctatttg tgctgcgagt ggtgttggag caccatttat
      301 gggagcatgt ggatacatag gagctaaatt tggtgtggat ctttgggcag gagtaacagg
      361 ggcaacgggt ggattttaat aaaaggagac ttgtatgaat acttattgta atataaatga
      421 aacaatgctg agtgaggttt atggaggtaa ttccggagga gcagctgtag ttgctgcttt
      481 aggttgtgca gcgggtggag tgaaatatgg gagacttcta ggaccatggg gcgctgcaat
      541 aggaggaatt ggaggagcag tggtttgtgg atatttagcc tataccgcta catcataaaa
      601 atgaaagcta aattttatga aggctatatt ctttattatt ctcttcgctt ttcaaaccta
      661 tcttatttat ctatccataa gtatctccga taaaaaacaa aagactattg aattaacatc
      721 attgaattgt tttgtcatat tatttctaat ctatgataaa ttaatttttc tctttattgc
      781 ctatgttttt ttgataattt ttatattaaa cttgttccgt aactgaaaag tgatatacta
      841 tatggataca aaaatgatgt cacaatttgc agttatggat aatgaaatgc ttgcttgcgt
      901 tgaaggtgga gatattgatt ggggaagaaa aattagttgt gcagcagggg ttgcatatgg
      961 cgcaattgat gggtgtgcaa caacggtttg acatttctat tgggaccatt tgctatagga
     1021 ataggtgtaa ctggtgctgc aggtggagct atacttggag gtgtagccta tgcagcaata
     1081 tgttggtggt aattatggat tttaaaagtt ttattattgg tttagtagtt ggtatatttg
     1141 gtccttatat ggatgactta attagaaaaa tattttcaaa atcttccaaa aaggatacgg
     1201 atagtactct ctaaaaaata aatttctccc ctgtatttgc tagcttaatt tacaggggag
     1261 tttctttaat attgtttcgc ctgtcaaaat gatatgtgat agcaggatga aaagtctaca
     1321 aacaagttgg tatcaataca atataaagag gaaagtatgt ttttaatgct tactttttaa
     1381 attttttaac aattcaagat gtttcgatga caattcaaga tttggatgaa aaattttaaa
     1441 aaacaatgat atactaaact tgtcaaagtt gcaacgggtg ctgctacatt atgttaaaaa
     1501 tcgctcttga ttgataagct ggatttgtta ggaaaggagt aaataattat ggataagaaa
     1561 aagatagttt cgactataat atgtatagta tttcttgtag tgtcagtaga taattttttt
     1621 agagacttaa ccccgttact gttcatttta aatattattg gcctgtcttg cttttcagtg
     1681 ctaacataca ttaacataaa agagatactc ttaaatataa gtaaatgatg atttggttag
     1741 tcctagaacc aatcaactta tgtttatcgg cttggcagat tttatgtttg tcatttgttt
     1801 gtatagaggt atttcagaaa cagaattcta tcaacaacta atcgcttata taggtgtatt
     1861 ctctgcttgt ttatcacgct tctgctcatg tggggcttga agtattataa aaagtagctt
     1921 tctatccttt tctgacagga tatttgagta gaaggctcta ttaaattgtt agtgaaataa
     1981 atgaatggag gtatttaata tgaaatacag attatttttt gttattttct tgagtagtgt
     2041 gttggatatt cttttaggga catttttaca aatctctatc gtatctattg ggtggcttgt
     2101 tctttacagt ggtttgtttg aagtaggagt tttccttctt gctaataaag gggtggcggt
     2161 aaaaatcaag gaagtagata ttcgaaatcg ctttaaattt atttttggaa aaaccttatg
     2221 gtttcaaatt cttttgctca tctttttgat aatcaaactt tatcttggtt tggatgcgag
     2281 gttgatttta ttctatggac atattttcat tgtctttaat gccttaatgt atctattatc
     2341 tagtagtcag gttagcctta aaaaaaaaca aactgtcttc ttaatcttac agcagtagag
     2401 tctgacaagg tgaggtcata gtagggagga tttctatgaa aaagtatcaa cttctattca
     2461 aaataagtgc agtcttctct tacttatttt tcgtatttgg tctttctcag ctgacgctta
     2521 tcgtccaaaa ttattggcaa ttttcttccc agattggtaa tttcgtctgg attcaaaata
     2581 tcttgagttt actttttatt ggagtcatga ttgtggttct tgttaagaca ggccatggtt
     2641 atctcttccg cattccaaga aaaaaatggc tttggtattc gattttgaca gtattagtgg
     2701 tagtgttcca gatctctttt aacgttcaga cagctaaaca tgttcagtca actgcggaag
     2761 gttgggctgt attgattggt tatagtggga ctaactttgc agagctaggt atttatatag
     2821 ccctgttctt tctggttcca ctgatggaag aattgattta tagaggatta ctgcaacatg
     2881 ctttctttaa gcattcgcga tttggtcttg atttgcttct tccttctatt ttatttgctc
     2941 tccctcattt ttcaagcctg cctagtctgt tagatatctt cgtctttgca acagttggaa
     3001 tcatctttgc tggtttgacc cgctatacca agagcattta tccatcctat gcggtgcatg
     3061 tgatcaataa tattgtagcg accttcccgt ttttgctcac ttttctacat agggtcttgg
     3121 ggtaaaaaaa caaaaggctt gcttttcagc catagaggag gtcatcatgt ataaacactt
     3181 atttttccta gattccaaaa ctttagatcg gttgacaccc tatattctag tcttggcttc
     3241 tgacaccatt gcttttaatg tttttgtgct aacctttgta tctgcggtgg tttttaattt
     3301 cctaaattcc atgctagctt taatggctat attcataggg gctggctatg tggtcggatt
     3361 ttggttgcta aaatggtttg ttttggaaag gttagagcta aaggatgacg cgtaggaagt
     3421 ctgtttggtg aggaggatag ttttatggag ttttttgata aatttcatgc cttgtgtttt
     3481 ggatttttag tactaataat tgtcattaca gttccttata cgattaacca tgggggtttt
     3541 tttcaaaatg aatctgcatt gattcttgta agtcttcttg taacctcgct gagtgttgct
     3601 tatgctagaa agtttgaaat gatttctttt gggatgttaa gcaagaaaca acttttgctt
     3661 ttcattgcaa tctttcttct aagtgtactt gagacgctgg tttatattca tttcttcgct
     3721 gtttcttctg gctcaggggt ccaacacttg gcggaagtca gcagaggaat ttccctgtct
     3781 ttgattttga ctacctcagt ttttggcccc atccaggagg aactcatttt cagaggactt
     3841 cttcaaggtg cggtttttga caattcttgg ttagggcttg tgctaacttc ctctctcttt
     3901 tctttcatgc atggaccttc taatgtccct tcgtttattt tttatctact tgggggtttg
     3961 ttgctgggct ttgcttataa aaagagtcaa aacctatggg tttctactct agtacacatg
     4021 ttttacaaca gttggccact cctatattat ttataaaaat tatgaaaagg taagcagaag
     4081 acggtgctta cctttttcaa gctataatga tacccaagcc aatccagagg cttttctttg
     4141 agaatcgggt tagagtggtt gacgaatagg ccaaaaacta gtagaatagt aaggaaactt
     4201 tatacggagg aaagaaatgg atttgggtga taatgagcta acactgactc ccatacctgg
     4261 gaaaagtggc aaggcttata tgggtagcta tcctgacggg aagcgcatct ttgtaaaaat
     4321 gaacacctct ccaatcctac ctggtctagc tagagaacaa attgctccac aattattatg
     4381 gagtcgtcgt ttggcagatg ggcgtgatat gtgtgctcaa gaatggttga caggcaagat
     4441 attgaccccc tatgatatga atcgtaagca aatcgtcaat attttaaccc gtcttcatcg
     4501 ctcacgtccg ttgatgacac aattgagtcg tttgggctat gccatggaaa cacctgtaga
     4561 tttactacag tcttggcagg aaacggctcc agatgctttg cgtaaaaatc attttatcag
     4621 tgaagtgatg gctgatttac gtcagactat tccaggattt agagaggacc atgcgaccat
     4681 tgtccatgga gatgtacgac atagtaattg gattgagaca gatagtggct tgatttattt
     4741 agtagattgg gattcggttc gcttgaccga tcgcatgttt gatgtggccc atatgctctg
     4801 ccattatatt tcagaacatc agtggaagga atggttgacc tactacggtt acaagtacaa
     4861 tcaaacggta ttaagtaaat tgtattggta tggtcaattg tcttatttga gtcagatttc
     4921 caagtattat atgaaccaag atttagaaaa tgtcaatcgg gagattcatg gtttgcgtca
     4981 tttccgagac aagtatggaa agagaagatg agagttagaa atcgtaaagg ggcaacagaa
     5041 ttactagagg caaatcccca gtatgtggtc ctcaatccct tggaagccaa ggcaaaatgg
     5101 cgggacttgt ttggcaatga taatcccatt catgtggaag ttggaagtgg aaagggtgcc
     5161 tttgtttcag gtatggccaa gcaaaaccct gacatcaact atatcgggat tgatattcaa
     5221 aagtctgttt tgagctacgc tttggacaag gtgcttgaag ttggagtgcc taacatcaag
     5281 ctcttgtggg tagatggttc tgacttaact gactattttg aagacggtga gattgatcgc
     5341 ttgtatctga acttttcaga tccatggccg aaaaaacgcc atgaaaagcg tcgtttgacc
     5401 tacaagacct tcttggatac cttcaaacgt atcttgcctg aaaatggaga aattcatttc
     5461 aagacggata accgtggctt gtttgagtac agtttagtga gcttttctca atatggcatg
     5521 aaactcaatg gtgtctggtt agatttgcat gccagtgatt ttgaaggcaa tgtcatgaca
     5581 gaatacgagc aaaaattctc aaacaagggg caagttatct accgagttga ggcagaattt
     5641 taagagatag cctaaaatta ggctgtacaa gtgcttttgc tttacataag ttggcaaacg
     5701 tgctatactg atagtaagaa tatgaaaagt gaggcgggga aatatcttcg cctcttgctt
     5761 atgaggaggt ggacgcaatc gcaacaatcg tagaattagt cagagaagtt gtagaacctg
     5821 tcatagaagc tccttttgaa ctcgtggata tcgagtatgg aaagattggc agtgacatga
     5881 ttctcagtat ttttgtagat aaacccgaag gaattacctt gaacgacacg gcagacttga
     5941 cagaaattat cagtcctgtc ctagacacca tcaagccaga tcccttccca gaacaatatt
     6001 tcctagaaat taccagtcca ggtttggaac gccctttgaa aaccaaggat gccgtcgctg
     6061 gagcggttgg aaaatacatc catgtcgggc tctaccaagc catcgataag caaaaggtct
     6121 ttgaaggaac cttgttggcc ttcgaagagg acgagttgac tatggaatat atggacaaga
     6181 cgcgtaagaa aaccgtccaa attccataca gtttagtatc aaaagcacgt ttagcagtta
     6241 aattatagaa aaagaaagga tagcttttga ggattcaaaa gtgaagaaaa catgagtaaa
     6301 gaaatgctag aggccttccg cattttggaa gaagacaagg gaatcaaaaa agaagatatc
     6361 atcgacgcag tagtagagtc gcttcgttcc gcttatcgca gacgctatgg tcagtcagac
     6421 agcgtagcta ttgacttcaa cgaaaaaaca ggtgacttta cagtttatac tgtccgtgaa
     6481 gttgttgatg aagtatttga tagccgtttg gaaatcagct tgaaagatgc tcttgccatt
     6541 aattcagctt atgaacttgg agacaaaatc aagtttgaag aagcaccagc tgagtttggt
     6601 cgtgtagcag cccagtctgc caaacaaacc atcatggaaa aaatgcgcaa gcaaacacgt
     6661 gccatcactt acaatactta caaagaacat gagcaagaaa tcatgtctgg tacagtagaa
     6721 cgctttgaca accgctttat ctatgtcaac cttggtagca tcgaagccca attgtcaaaa
     6781 caagaccaaa ttcctggaga agtttttgct tctcatgatc gtatcgaagt ttatgtttac
     6841 aaggttgaag acaaccctcg tggtgtgaac gtctttgtta gccgtagtca tccagaaatg
     6901 atcaaacgtt taatggagca agaaattcca gaagtttatg atggaactgt tgaaatcatg
     6961 agcgtggctc gtgaagcagg tgaccgtacg aaggttgctg ttcgtagcca caatccaaac
     7021 gtggatgcta tcggtacaat cgttggacgt ggtggtgcta atatcaagaa gattactagc
     7081 aaattccacc cagctcgtta cgatgctaaa aatgaccgca tggtaccaat cgaagaaaat
     7141 atcgatgtta tcgagtgggt agcagatcca gctgaattta tctacaatgc catcgctcct
     7201 gctgaggttg accaagttat ctttgatgaa aacgacagca aacgtgcctt ggtggttgtt
     7261 ccagataaca agctttctct tgccattggt cgtcgtggac aaaacgtgcg cttggcggct
     7321 cacttgactg gttaccgtat cgatatcaag tctgctagcg aatttgaagc catggaagac
     7381 gctgcttcag tagagttgga agtagaaaac gatactgtag aagaataaaa gctgctagag
     7441 gagggaaaga tgaaaacaag aaaaatccct ttgcgcaagt ctgttgtgtc taacgaagtc
     7501 attgataagc gtgatttgct ccgcattgtc aagaacaagg aaggacaagt ctttattgat
     7561 cctacgggca aggccaatgg ccgcggcgct tatatcaaac tagacaatgc agaagcccta
     7621 gaggcgaaaa agaagaaggt ctttaaccgc agctttagca tggaagtgga agaaagcttt
     7681 tatgacgagt tgatcgctta tgtggatcac aaagtgaaaa gaagagagtt gggacttgaa
     7741 taagcaaaag ataagtaatc tcttggggct tgctcagcga gcagggcgca tcatatcggg
     7801 tgaagaattg gtggtcaagg ccattcaaga cggcaaggcc aagttggtct ttctagctca
     7861 tgatgctgga cccaatctga ccaagaagat tcaagataaa agtcattatt atcaagtaga
     7921 aattgtaacc gtgttttcaa cactggaatt aagcatagca gtcgggaaat cgagaaaggt
     7981 tttggctgta acagatgctg gatttacaaa gaaaatgagg tctcttatgg aatagaagag
     8041 gaggacatga tttgtctaag aaaagattgt acgaaatcgc aaaagaactt ggaaaagaaa
     8101 gtaaagaagt tgtagcgcgt gcaaaagagt tgggcttgga tgtgaaaagc cactcatcaa
     8161 gtgtggaaga agctgtcgct gcaaaaattg ctgccagctt taagcctgca gctgctccga
     8221 aagtagaagc aaaacctgca gcaccaaaag taagtgcaga aaagaaagcc gaaaaatctg
     8281 agccagctaa accagctgta gctaaggaag aggcaaaacc ggctgagcca gttgctccga
     8341 aaacagaaaa agtagcagtg aaaccgcaaa gtcgtaattt caaggctgag cgtgaagccc
     8401 gtgctaaaga gcaggcagaa cgacgcaagc aaaataaggg caataaccgt gaccaacaac
     8461 aaaacggaaa ccgtcagaaa aacgacggcc gtaatggtgg aaaacaaggt caaagcaacc
     8521 gcgacaatcg tcgctttaat gaccaagcta agaagcagca aggtcagcaa aaacgtagaa
     8581 atgagcgccg tcagcaagag gataaacgtt caaatcaagc ggctccacgt attgacttta
     8641 aagcccgtgc agcagcccta aaagcagagc aaaatgcaga gtacgctcgt tcaagtgagg
     8701 aacgcttcaa gcagtatcag gctgctaaag aagccttggc tcaagctaac aaacgcaagg
     8761 aaccagagga aatctttgaa gaagcggcta agttagctga acaagcacag caagttcaag
     8821 cagtggttga agtcgtccct gagaaaaaag aacctgcagt ggatacacgt cgtaaaaaac
     8881 aagctcgacc agacaaaaat cgtgacgatt atgatcatga agaagatggt cctagaaaac
     8941 aacaaaagaa tcgaagtagt caaaatcaag tgagaaatca aaagaatagt aactggaata
     9001 acaacaaaaa gaacaaaaaa ggcaataaca agaacaaccg taatcagact ccaaaacctg
     9061 ttacggagcg taaattccat gaattgccaa cagaatttga atatacagat ggtatgaccg
     9121 ttgcggaaat cgcaaaacgt atcaaacgtg aaccagctga aatcgttaag aaacttttca
     9181 tgatgggtgt catggccaca caaaaccaat ccttggatgg ggaaacaatt gaactcctca
     9241 tggtggatta cggtatcgaa gccaaacaaa aggttgaagt ggataatgct gacatcgaac
     9301 gtttctttgt cgaagatggt tatctcaatg aagatgaatt ggttgagcgt ccaccagttg
     9361 ttactatcat gggacacgtt gaccacggta aaacaaccct tttggatact cttcgtaact
     9421 cacgtgttgc gacaggtgaa gcaggtggta ttactcagca tatcggtgcc taccaaatcg
     9481 tggaaaatgg taagaagatt accttccttg atacaccagg acacgcggcc tttacatcaa
     9541 tgcgtgcgcg tggtgcttct gttaccgata ttacgatctt ggtcgtagcg gcagatgacg
     9601 gggttatgcc tcagactatt gaagccatca accactcaaa agcagctaac gttccaatca
     9661 tcgtagccat taacaagatt gataaaccag gtgctaaccc agaacgcgtt atcggtgaat
     9721 tggcagagca tggtgtgatg tcaaccgctt ggggtggaga ttctgaattt gttgaaatct
     9781 cagctaaatt caaccaaaat atcgaagaat tgttggaaac agtccttctt gtagctgaaa
     9841 tccaagaact caaggcagac ccaacagtgc gtgcaatcgg tacggttatc gaagcgcgct
     9901 tggataaagg aaaaggtgcg gtcgcaaccc ttcttgtaca acaaggtacc ttgaatgttc
     9961 aagacccaat cgttgtcgga aataccttcg gtcgtgtccg tgctatgacc aacgaccttg
    10021 gtcgtcgtgt taaagttgct ggaccatcaa caccagtctc tatcacaggt ttgaacgaag
    10081 caccgatggc gggtgaccac tttgccgttt acgaggatga aaaatctgcg cgtgcagcag
    10141 gtgaagagcg tgccaaacgt gccctcatga aacaacgtca agctacccaa cgtgttagcc
    10201 ttgaaaacct ctttgatacc cttaaagctg gggaactcaa atctgttaat gttatcatca
    10261 aggctgatgt acaaggttct gttgaagccc tttctgcctc acttcaaaag attgacgtgg
    10321 aaggtgtcaa agtgactatc gtccactcag cggtcggtgc tatcaacgaa tcagacgtga
    10381 cccttgccga agcttcaaat gcctttatcg ttggtttcaa cgtacgccct acaccacaag
    10441 ctcgtcaaca agcagaagct gacgatgtgg aaatccgtct tcacagcatt atctacaagg
    10501 ttatcgaaga gatggaagaa gctatgaaag ggatgcttga tccagaattt gaagaaaaag
    10561 ttattggtga agcggttatc cgtgaaacct tcaaggtgtc taaagtggga actatcggtg
    10621 gatttatggt tatcaacggt aaggttgccc gtgactctaa agtccgtgtt atccgtgatg
    10681 gtgtcgttat ctatgatggt gaactcgcaa gcttgaaaca ctataaagac gacgttaagg
    10741 aagttacaaa cggccgtgaa ggtggattga tgatcgacgg ctacaatgat attaagatgg
    10801 atgatgtgat tgaggcgtat gtcatggaag aaatcaagag ataagatttt ttgctccttt
    10861 cttaggtggt gagggacgca agcaaaccga tggtttcatt gcttattttt gagcctaggg
    10921 tctcaaaaat cccctgtgat gggactgata aatcagttcc atcactttca ccacggcgaa
    10981 agaagcagat gacttcaaat tgaacttcgt ttcaatttaa actgaaaatc aagaagttta
    11041 aaatagctag gtctgctggc ctagcttttg gttcaaagta gagaaaggaa tatcatggca
    11101 aatcatttcc gtacagatcg tgtgggcatg gaaatcaagc gtgaagtcaa tgagattttg
    11161 caaaagaaag tccgtgatcc acgtgtccaa ggtgtgacca tcacagatgt tcagatgctg
    11221 ggtgacttgt ctgttgccaa ggtttattac accattttga gtaaccttgc ttcggataac
    11281 caaaaagccc aaatcgggct tgaaaaagca actggtacca tcaaacgtga acttggtcgc
    11341 aatttgaaat tgtacaaaat cccagatttg accttcgtca aagacgagtc catcgagtat
    11401 ggaaacaaga ttgacgagat gctacgcaat ctggataaga actaaagaag aggggttgcc
    11461 cctcttttt
//
LOCUS       DAVBHS010000058        11343 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVBHS010000058 DAVBHS010000000
VERSION     DAVBHS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11343
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.058"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..1017)
                     /locus_tag="VQ651_001108"
     CDS             complement(<1..1017)
                     /locus_tag="VQ651_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2754116.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMKELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     KGEILEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESSEKFEAIKQNLVDEMWKVAQRELATGFYYGTPSENEQLFGARRKIPEYKFVAEVV
                     SYDDA"
     gene            1641..1823
                     /locus_tag="VQ651_001109"
     CDS             1641..1823
                     /locus_tag="VQ651_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET2754117.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            1833..2198
                     /locus_tag="VQ651_001110"
     CDS             1833..2198
                     /locus_tag="VQ651_001110"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET2754118.1"
                     /translation="MPNSENTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
     gene            2195..3385
                     /locus_tag="VQ651_001111"
     CDS             2195..3385
                     /locus_tag="VQ651_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET2754119.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
                     RNGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSS
                     NLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNEC
                     LNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
                     QQLL"
     gene            complement(3900..4892)
                     /locus_tag="VQ651_001112"
     CDS             complement(3900..4892)
                     /locus_tag="VQ651_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2754120.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
     gene            5052..6806
                     /locus_tag="VQ651_001113"
     CDS             5052..6806
                     /locus_tag="VQ651_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754121.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAVLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIVTPILVLVLGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAEVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAIIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            6790..8511
                     /locus_tag="VQ651_001114"
     CDS             6790..8511
                     /locus_tag="VQ651_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754122.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERIHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            8522..9151
                     /locus_tag="VQ651_001115"
     CDS             8522..9151
                     /locus_tag="VQ651_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET2754123.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALIALGAFLGGILGAYIGKALLKKHFSNGLYCVGYFTPCLILWCYLN"
     gene            9052..9609
                     /locus_tag="VQ651_001116"
     CDS             9052..9609
                     /locus_tag="VQ651_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2754124.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGINFKNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            9597..10961
                     /locus_tag="VQ651_001117"
     CDS             9597..10961
                     /locus_tag="VQ651_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754125.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGSGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDILEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDESSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAI
                     ASVMCKTSPFIFFDEPSSGIDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADE
                     IFEL"
BASE COUNT         4146 a         1616 c         1920 g         3661 t
ORIGIN      
        1 cgcatcatca taagaaacca cttcagcgac aaacttgtac tcaggaattt tacgacgagc
       61 accaaacaac tgctcatttt cagatggtgt accatagtaa aatcctgtag ccagttcacg
      121 ttgggcaacc ttccacatct cgtccactaa gttttgtttg atggcttcaa acttttcaga
      181 actttcaaga taggcatcca cagccgcctt gtagcagttg gttactgttg atacgtagtg
      241 aatagacttc atacgtcctt cgatttttag actgtccaca ccattttcaa tcatatctgg
      301 aatgtggtca atcatagaca tatcaacggc tgacattgaa aattcttcta gaatctcacc
      361 cttcaaactc ttacgttctt tcccaaatgg catatcgtaa aggtcgtatt tccaacggca
      421 tgactgagaa cacccaccac ggttggcatc acgcatactc atgtggtttg aaagagtaca
      481 acgtccagag tatgaaatac acatagctcc atggacaaag gcttcaattt caacatctgt
      541 acgtttgcgg atttcagcta attctttcat tgaaacctca cgtgctaaaa cgacacgagt
      601 caagcctagc tctttccaga actcaagggt ttcatagtta gtggcactgg cttgggtaga
      661 aaggtggatt tcaaggcctg gtgcttcagt cgctgcaatc ataatcaagg ctgggtcaga
      721 tacgataact gctgcaatcc cgatatcacg cagtttacga aaccactcac cagcaccagc
      781 ttcatttcct tcgtgcataa ccatattagc cgctacatag accttggcac catacttggc
      841 cgcaaactgc acgccttctt ccatctgttc gaaagtaaag tttcccgcac ggctacgaag
      901 accataggcc tgaccaccga taaagacagc atctgctcca tactgaacag ctacctttag
      961 cttctctaaa gtccctgcag gtgataaaac ctcaggacgt tttaatgttt ttgtcatgat
     1021 ttctcctatt tgcaatataa aagtttgaca tttttccttc tcattttata gtaaataaag
     1081 gataaaatca agcctagacg gattgttttg acaattctaa tcttttagac tttgcataat
     1141 aactattaaa cagtcgaaaa ttaccccagt tttaacaagt agtcaaaaag tctaaaaaag
     1201 taaaaaccag tatcccaaga tactgactcg taaaaatata taaattagtc cttagatgac
     1261 tactcccact ctgttgctcc tataatggaa aaggattgat acagtctaga atatcgcttt
     1321 tttatttatt attctgatat aaaactgtta gaattgattt tttgttttat acccaaatat
     1381 gaatagagac gtagctaata agttgaataa catatttgga aaaatgagaa atagcaatat
     1441 tactagaact tttagaaaca atcaatattt aaaaagaaaa aagctcaaag aatttattaa
     1501 accatgttat aatagatata gctataattt tatttataac aagaggatag aaatgaccga
     1561 attagaaaga aaaaatcgaa aaattagcta agaaatattc tgataactta aacatcaaag
     1621 ttcaagagag agttcgtgaa atggcaaatg ataataagag ccattatttg atatacagag
     1681 ttttaggtat ttcatttgaa gaaggagaaa atatcgattt gtatcaaaat aaaggtcgtt
     1741 ttttatacaa atatgctggt tcatttttag aagaagctgc agtactatcc tttaacgaaa
     1801 aatttggtac agaaaatact taaaaagtta acattcctaa ttctgaaaat acaaaaccta
     1861 agacttttga aattgattgt ttagtcggag aaaaacacgc atacgaaata aaatggtggg
     1921 atgcaactac agatggagac catataacta aagaacacac tagaataaaa gttattcata
     1981 acaaaggata tataccaatt cggttaatgt tctactatcc aaatagaact caagctataa
     2041 aaattcagca aactttagaa acattgtata acggtattgg agggaaatat tattatggag
     2101 attctgcctg ggaacattta agagcagtga ccggtattga tttacttagt attctaacag
     2161 atattgcaaa taaaaaaaca ggggtaaaat caaaatgaca gtattaaaag gagataactt
     2221 agaaatatta aaaactattg aatcctcaag tattgattta atctatatgg accctccttt
     2281 ctttacacag aaaacccaaa aattatctaa taacaaaaat attatgtatt cattcgaaga
     2341 tacgtggact tcgattgagg attacaaaga atttttgtct gtaagattag aagaatgcaa
     2401 aagagtgcta aaaaatagtg gcagtatttt cgttcattgt gataaaattg caaatcatca
     2461 tattagatta attttagata atatctttgg agcagatatg tttcaaagcg aaattatatg
     2521 gaactataaa cggtggtcta attcaaaaaa gggattattg aacaatcatc aaaacattta
     2581 cttttattca aagtcaaaag attttaaatt taatacaatt tttacagagt attcttctac
     2641 tacaaatatc gaccaaatac tagtagaacg aaaacgaaat ggaaactcta aaactatata
     2701 taaagttgat aataatggta actatattct agcaaaagag aaaaatggag ttcccctttc
     2761 agatgtttgg aatataccat ttcttaatcc aaaagctaaa gaaagagtag gttatcctac
     2821 acaaaaacct attctgttat tagaacaaat tataaagatt gctactgata aaaatgatat
     2881 agttttagac ccgttctgtg gaagtggaac tactttagta gcctccaaga ttttgaatag
     2941 aaattatatg gggattgatt tatctgagga agctatcaat ataactcagc aacgtctgga
     3001 aaatgttata aaaacaagtt caaatttatt gaataaagga atcgaagcat atagaaccaa
     3061 aactgaggaa gaggaaaaca ttcttaaatt attacaggca aaaattgttc aaagaaataa
     3121 aggaattgat ggttttttac ctaaacattt tcaaaaaaaa ccgataccta taaaaattca
     3181 aaaaaataat gaatgtctga atgagagtat ctctttatta cagaatgcta taaactccaa
     3241 aaaacttgat tttggagtag ttataaaaac tcattcagat aatttattat ttgattttga
     3301 tacgattcct gaaaatataa tagtagttga ccattttgaa cttacaatcg aaaaatggtt
     3361 aagtaaaagt caacagttac tatagtttgc aaattattta cagaaagaac aaaaaatgct
     3421 ctgaaacact gatttcaagg cattttttgt tagtttaaaa ttactcccat tcttcttttc
     3481 caaacgtaca atatatccaa aaccattcaa aatactagat tctatttttt ataatatcac
     3541 taaatccacc taattatagg acgttttcag atttttagtc ccgtcccagt cccagtaccg
     3601 gagaaatatt gttttaatat aatatctctt tttgtcttct aagctcttaa aagcaaaaga
     3661 acaagtaaag agtcaagaca aggataaaaa gtccatatta gggcaaataa aaagctttaa
     3721 gacagatgac aaatctaagt caaataagaa agaccatagc aaaggtgcag agagataaat
     3781 attggcggtc ttcggactgc ctttattttt ttatccattt ttcaaatcaa atttattcag
     3841 actatatatg cacatataca cttaaattca tataaaaaca tggcttgtaa aaaattactt
     3901 taatcacaat aatcgcattt aaaattgtga tgtttgcaag ctaaattacg gacttcactt
     3961 ggaagttttc ccttgtatct tttataatag atagaaaatt tgctggcaga tgaatatcca
     4021 acagattctg ctatctcttt tataggtagt tcagtgttta aaagaagagt ttcagctaca
     4081 ttcattcttt ttctttgagt gtactctgta atgctttgac aatatttttc cttaaataaa
     4141 ttttttaatt tagtaccact cattttagat attttttcaa gcgtgccttg atttacattc
     4201 gttgcaaaat gatcatctaa gaatcttgct acatcttcaa gtgctttatc atcatcaatt
     4261 tcaattttat atttttttct atttaagtat gtgtcaatta ctatacttat ccattcattt
     4321 gcctttgctt taaagaaaaa atcagcggca ggagcgtcca tcttacaatt taatatttcc
     4381 attgccactc tttctaaggc ctttgtaagt attatttgat tcggttgaag caaggttgaa
     4441 taaaaagatt ctggattaat gttaatagat gctaaatgtt tttctattag ctctttttta
     4501 aaaccaatgg aaacagcaag ataacaacaa ttctcgtgta ataaaaaaac aaaatcatct
     4561 tttatattat caaaatcaaa agtacataaa gagtttgcgg taatagtttg atacggatta
     4621 aacttttctc cgtttgcact gacaatgtaa cttgaataaa ttgaaacata gtctgacata
     4681 ctataagtgc tattttgaac tacttcctct ttgatataaa aatcatgtat atcgataatg
     4741 aagctgcctc cttcataaaa ccaatatagg ccttctgcat aagttgaatt ctcttttttc
     4801 caacaaaatg tatgacctgc acttgaatac ttctcattgt ttttacattc atctactttc
     4861 atataatctt ttacaaaatc ataatatgac ataacacact atccctttta gacaatattc
     4921 caattagcct tattaattca aaactattgt attagtaatt ataacagatg tataatagaa
     4981 aagcaatgat agatattatc aattaagcga atttatatct aaaagggata ttaaagaaag
     5041 gagatatgct tatgaagatt tacaaaaaac tatttgctta tgtccaagat aagaaatatc
     5101 ttggggtttt ggccataatt ttttctgcta tatctgctgt acttacagta tatggatatt
     5161 atttaatcta caaatttcta gataagttaa taattaattc aaacttatcc ggtgcagaga
     5221 gtatagcatt aaaatctgtt attacactaa caagtggagc gatattttat tttgtctcag
     5281 gaatgttttc acatatcttg ggatttaggc ttgaaacaaa tttaagaaaa aggggaatcg
     5341 atggtctgga aaaagcaagt tttaggttct ttgacttaaa tccatctggt caaataagaa
     5401 agatcataga tgataatgca gcacaaactc atcaggtggt agctcacatg attccagata
     5461 gctctcaggc aatagtcaca cctatacttg tccttgtact tggctttata gtaagtataa
     5521 gagttggcat aattttgctt gctcttacta taattggtgg cttaatttta ggggcaatga
     5581 tgggcgagca agaatttatg aagatatacc aagaatccct atctaaacta agtgctgaaa
     5641 ctgttgagta cgtgagagga atgcaagttg taaaaatatt taaagcaaat gtagagtctt
     5701 ttaaaagctt ttataaggcg ataaaagatt actcaaagta tgcttatgat tattccctat
     5761 cttgtaaaag gccttatgtt ttgtatcaat ggttattttt tggactgatt gcaattttaa
     5821 ttattcctat agtttatttt atgactagct tagctagcgc agaggtgatt ttacttgagc
     5881 ttatcatgat tttattttta tcaggagttc tctttgtttc attcatgaga atgatgtggt
     5941 actccatgta tatttctcaa ggaaattatg cagtagatac tttagaggcg ctttacgaag
     6001 atatgcaaaa agacaaatta gtgcatggta atgtcaataa ttttaaaaac tataatatag
     6061 aatttgagaa tgttagcttt gcttataatg ataaagctat cattgaaaat ttatccttta
     6121 atttagaaga aggaaagtcc tacgcacttg tcggttcatc tggatcaggc aaatcaacag
     6181 tagcaaaact tatatcaggt ttttacaatg ttaataaagg aagcataaag ataggcggga
     6241 tagcaataag tgaatattct gacgaagcct taattaaagc catttccttt gtttttcaag
     6301 attcaaaatt attcaagaag agcatttatg ataatgtagc tttagctaat aaagatgcga
     6361 cgaaagatga cgttatgaga gccttaaaat tagcaggatg cgatttaata ttagacaaat
     6421 tcccagaaag agaaaataca atcataggct caaaaggtgt ttatttatcc ggtggagaaa
     6481 aacaaagaat tgcaattgct agagcaattt taaaggattc caaaattatt attatggatg
     6541 aagcatcagc atctattgac ccagataacg agtttgaatt gcaaaaagct tttaaaaatc
     6601 ttatgaagga taaaacagtt atcatgattg cacacaggct atctacaatt aaagaccttg
     6661 atgaaattat tgtcatggat agtggaaaaa ttatagaaag agggtctgac aaagaattaa
     6721 tgtcaaaaga tacaaggtat aagagcctgc aagagatgtt taacagtgcg aatgaatgga
     6781 gggtttcaaa tgaaagagtt ttataaaaaa agatttgctc ttacagatgg aggagcaaga
     6841 aatttaagta aagcaacact ggcttcattt ttcgtttatt gtataaacat gcttcctgcc
     6901 atattactta tgatttttgc tcaggaagtt ttggaaaata tgggcaaaag caatggcttt
     6961 tatatagtat tctcagtttt gactttgata gcaatgtata ttttgctttc tatcgaatac
     7021 gataaattat ataacacaac ctatcaagaa agtgcagatt taagaataag gacagcggag
     7081 aatttatcaa aattacctct atcttacttt tctaaacatg acatttccga catttcacaa
     7141 acaatcatgg ctgatattga aggcatagag catgcaatga gccactcaat accaaaggtg
     7201 ggcggcatgg tactgttttt cccattaata tctgtaatga tgctagcggg caatgtcaag
     7261 atgggtttag ctgtaattat tccatctatt ttaagcttta tatttatacc tttatctaaa
     7321 aaatatcagg ttaatggaca gaatagatat tatgatgtct taagaaaaaa ctcagaaagc
     7381 tttcaagaaa atatcgaaat gcaaatggag attaaagcat ataatttatc gaaggatatt
     7441 aaagatgact tatataaaaa aatggaagat agtgagagaa tacacttaaa ggcggaagta
     7501 actacaattt taactttgtc tatatcttca atatttagct ttatatctct tgctgttgtg
     7561 atatttgtcg gcgtaaatct aattattaat aaagagataa attctctcta ccttatagga
     7621 tatttactag ctgctatgaa gataaaagac tctttagatg catctaaaga gggcttgatg
     7681 gaaatatttt atttatcgcc caaaatagaa agattaaaag aaattcaaaa tcaagattta
     7741 caagaaggcg atgactatag cttaaaaaaa tttgatattg atctaaaaga tgttgagttt
     7801 gcctacaata aagacgcaaa agttttaaat ggtgtaagtt ttaaagctaa gcagggagag
     7861 gtcactgctt tggtaggtgc aagtggctgc ggtaaaacaa ctatcttgaa acttatatca
     7921 agactttatg attatgacaa gggacaaatc ttaatcgatg gcaaagatat aaaagaaata
     7981 tcaacagaat ctctttttga taaggtctct atagttttcc aagatgtggt tctctttaat
     8041 caaagcgtta tggaaaatat tagaatcggt aagcaagatg caagtgacga agaggttaaa
     8101 agagcagcaa aacttgcaaa ttgcacagat tttatagaaa aaatggataa aggtttcgat
     8161 acagttattg gtgaaaacgg agctgagcta tcaggaggag aaagacaaag attatcaata
     8221 gccagagcct tcttaaaaga tgcgccgata ttgatcttag atgagataac agcaagcctt
     8281 gatgttaaca acgagaaaaa gattcaagag tctttaaata atttagttaa agataaaact
     8341 gttgtaatca tttcacatag aatgaaatcc atagaaaatg cagacaagat agtagttctt
     8401 caaaacggaa gagtagaaag cgaaggtaag catgaagagc ttttacaaaa atcaaaaatt
     8461 tacaaaaatt taatagaaaa gacaaaaatg gcagaagaat ttatttatta ggaggactac
     8521 aatggataat aaaaaattaa aagtaaaaga tttagtaagt atcggtgttt ttggcgtaat
     8581 ttattttgcc ttcatgtttg gagttggtat gatgggcttg attccaatat tgttcttaat
     8641 atacccgaca gtattagcca tagttgcagg aactgttgtt atgttattta tggctaaggt
     8701 tcaaaagcca tgggcactat ttatatttgg tatgatatca ccacttgtga tgtttgcagc
     8761 tggtcatacc tacgtagttg tggttttatc acttatagta atgataatag cagaattaat
     8821 tagaaagatt ggtaattata attcatttaa atacaatatg ctttcttatg caatcttcag
     8881 cacatggata tgtagctctt taatgcaaat gcttttagca aaagaaaaat atatggagtg
     8941 gtctttgatg acgatgggaa aagattatgt tgatgtatta gaaaagttaa taacttatcc
     9001 tcacatggct ttaatagcct taggtgcttt cttaggagga attcttggag catatatagg
     9061 caaggctcta ttgaaaaaac acttttcaaa tggattatat tgtgtgggat actttactcc
     9121 ttgcctaatt ttatggtgtt atctgaatta aaccctatag ttaagatgtt tttgagtata
     9181 cctattgtta ttagaatgtt tattttacca tttatggcag caagctttat gataaagacc
     9241 tcggatgtag gcgcaataat ttcatcgatg gataagctta agatttcaaa gaatgtatcc
     9301 atacctattg cggttatgtt tagattcttc ccatctttta aggaggagaa gaaaaacatc
     9361 aaaatggcta tgagagtaag agggataaat tttaaaaacc cagtcaaata tcttgaatat
     9421 gtttctgtgc cactactcat tatatcatct aatatatcag atgacattgc aaaagcggca
     9481 gaaacaaagg caatagaaaa tccaattgcc aagaccagat acattcgcgt aaagatacag
     9541 ctaattgatt ttgtttatgt tttagcggtt gctggactta ttgtgggagg cttaatatgg
     9601 ttgaaataaa aaatttaagt cttgattatg gtgaagagca tatattagat gatatatcac
     9661 tatccatagc cgagggagag tgcgtgctat ttacaggaaa aagtggaagt ggtaagtcat
     9721 ctttaataaa ttcaatcaat ggactagctg taaggtatga taacgcaaag acaaagggcg
     9781 aaataattat tgatggtaag aatataaaaa atttggaact ttatcaaatc tcaatgcttg
     9841 tttcaactgt ttttcaaaat cctaagacat atttttttaa tgtcaatacg acattagaat
     9901 tattatttta tttggaaaat atcggtcttg caagagaaga gatggacagg cgtttgaagg
     9961 atatacttga gatattcccg ataaaaaatc ttttgaacag aaatatattt aatctatccg
    10021 gcggtgaaaa acaaattctt tgcattgcag cttcttatat agcaggtaca aagattatag
    10081 ttatggatga gtcttcatcg aatttagata ttaaaagcat aagtgttttg gcaaagatgc
    10141 taaagatatt aaaagagaaa ggcataagca taattgttgc agagcataga atttattatt
    10201 tgatggacat agttgaccgt gtatttttaa tagataaagg aaagcttaaa aaaacttata
    10261 ctagaagtga atttttaaag ctagataaaa atgaattaaa tgctttaagt ttaagagata
    10321 aagaattaag taaattaaaa gttccttatt taaaagaagg tggagagtat cagataaaaa
    10381 atcttagcta caaatttact gatgatgagt gtttaagctt aaaagatatt tcgttcaagc
    10441 ttgggaaaat ttatggcata ataggatcca acggacgagg aaaatcaacg cttttaagat
    10501 gtttaatagg tcttgagaaa aaatcaaaag aagaaattta ttttaaggga gagaagctat
    10561 ctaaaaaaga aagactcaaa aactcttcac ttgttatgca agatgtaaat catcaattat
    10621 tcacagatga agtattcaac gagcttagat taggagtaaa gaattttgat gaagaaaagg
    10681 cgaaaatcat tttaaaagat ttaggcctgg acgaatttat tgaaaggcat ccgatgagtt
    10741 tatcaggagg gcaaaagcaa aggcttgcaa tagcatctgt tatgtgcaag acttctccat
    10801 ttatcttttt tgacgaacct tcaagtggta tagattattc caatatgata aaaatatctg
    10861 aactgattaa taagtataaa accatggata aaataatttt tattgtttcc catgatatag
    10921 aatttttaaa tgaagtggca gatgaaattt ttgaattgta aaatagagtc tgttaaacat
    10981 gtctatagaa ctggtaaatt gtctaaaaat ttaaaagata ctaacacata ttgatattta
    11041 ttagaaaatt aacttcagga tttttacagt atttataaac tttataatat gtatttatag
    11101 tattaataat ttccatatct ttagggaaat tatttattta cttaccataa ttaaagagat
    11161 gagagataaa tatcggcagt cttcggactg cctttatttt tttacttaat ttaatgcagt
    11221 aaatcatacg attgaaagga atctgcgtta tatgctataa ttaggtagtt aatctataca
    11281 tttagaaagg taaaatatag aattggagaa tgatatggga ttttcatata ataaattgta
    11341 acc
//
LOCUS       DAVBHS010000059        11204 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVBHS010000059 DAVBHS010000000
VERSION     DAVBHS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11204
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.059"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(308..1474)
                     /gene="recA"
                     /locus_tag="VQ651_001118"
     CDS             complement(308..1474)
                     /gene="recA"
                     /locus_tag="VQ651_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET2754126.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            complement(1529..2785)
                     /locus_tag="VQ651_001119"
     CDS             complement(1529..2785)
                     /locus_tag="VQ651_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET2754127.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNLLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQVDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGISSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(2869..3885)
                     /locus_tag="VQ651_001120"
     CDS             complement(2869..3885)
                     /locus_tag="VQ651_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET2754128.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEKALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(3893..4411)
                     /locus_tag="VQ651_001121"
     CDS             complement(3893..4411)
                     /locus_tag="VQ651_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2754129.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(4401..4844)
                     /gene="tsaE"
                     /locus_tag="VQ651_001122"
     CDS             complement(4401..4844)
                     /gene="tsaE"
                     /locus_tag="VQ651_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET2754130.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(4930..5550)
                     /locus_tag="VQ651_001123"
     CDS             complement(4930..5550)
                     /locus_tag="VQ651_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754131.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(5933..6796)
                     /locus_tag="VQ651_001124"
                     /pseudo
     CDS             complement(5933..6796)
                     /locus_tag="VQ651_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7470..7766
                     /locus_tag="VQ651_001125"
     CDS             7470..7766
                     /locus_tag="VQ651_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2754132.1"
                     /translation="MLKFRKVVASSLAVLAFSLALPVLAVSHRGGEWTYGGHHDPNNW
                     GAFSNYYHGSQYHWAYVGSNGRNNQKTVYAGARSAAYAFVNTNFGEQVTFDAGW"
     gene            7869..9884
                     /locus_tag="VQ651_001126"
     CDS             7869..9884
                     /locus_tag="VQ651_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2754133.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSYYYPNVVVHDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFIALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAGLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            9886..10518
                     /locus_tag="VQ651_001127"
     CDS             9886..10518
                     /locus_tag="VQ651_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754134.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLTYLDLDKRIFELSGGESQRVALAKVILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDRLK"
     gene            10659..10937
                     /locus_tag="VQ651_001128"
     CDS             10659..10937
                     /locus_tag="VQ651_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HET2754135.1"
                     /translation="MKKFDNYIVEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
BASE COUNT         3421 a         2236 c         2009 g         3538 t
ORIGIN      
        1 taaagctatt atatcagctt tccagaggag cgacaagaaa cagcaaactt atatatagcg
       61 gaatgaaata aggaaagtca aatcgatttt taacaatgtt ttagaagtag atatgtacta
      121 ttctagtttc gatctattat agttggttga gtttggaata gtacgctgta gctgctaaaa
      181 catttctaga aattaatttg actttcctaa tagagttgtt catatcttat ttcaagctac
      241 tatatataat ctaaaaactt gaatcaaaaa tcgaaaaagt agcggatttc tccactgctt
      301 taacagctta ttcttcaatt tcgatttcaa gttcatcgcc taagtcaagc ggaacttctt
      361 cattcactgc ttcttctttc tttggctcat cttttttgtt ttcagtatct tgttctgaaa
      421 cttcttctcc atcaatcaag ccaaatttag aacggacttg cttatcaatt tcatcaaaga
      481 tttctgggtg ctctgccaag tatttcttag cattctcaga accttgccca attttttcat
      541 ctttgtaaga ataccaagcc cctgcttttt tgataatatc caaatcgctt gcaatcttca
      601 aaagctcacc agtcttagaa attccttctc cgtacataat ttcaactacg gcttccttaa
      661 acggtggagc taccttattt tttacaacct taatcttagt ttctttaccg acattggttt
      721 ctttttggtc accagttccc ttaatttgtg tattaccacg aacatccaag cggactgaag
      781 catagaattt caaagcacgt ccgcccggtg ttgtttctgg atttccaaac atcactccaa
      841 ctttttcacg caattggttg ataaaaatgg caattgtttt ggttttattg atagaggcgc
      901 caagtttacg catggcctgg ctcatcatac gagcctgcaa accaacatgg ctatctccga
      961 tatctccatc aatttccgca cgaggaacaa gggcagcaac tgagtcgact acgacaagat
     1021 caactgcacc tgagtcaatc aattttcccg caatctcaag accttgctct cctgagtctg
     1081 gttgagacaa gagcaattcg tcaatattga caccaagggc cgcagcataa gctggatcaa
     1141 gggcatgttc cgcatcgata aaagcagcaa tcccaccttc tttttgcgct tgtgcaactg
     1201 catgaagggc aaccgttgtc ttaccagatg actctgggcc atagatttcg atgatacgtc
     1261 ccttaggata accacctgag ccaagggcaa tgtcaagagc taaagaacct gagctcatca
     1321 cttgcacctt ttgctccgca cgttcaccca aacgcatgat tgatccttta ccaaagtctt
     1381 tctcaatcaa tttaagagcg tcattcaagg ccttttcacg ttctgcccca aatttttttg
     1441 aaatttcttc taattttttt ggttttttcg ccattctatt ctcctacatt ctaatgatcc
     1501 tcagacctat ctactattat atcaaaaatt agtcacttaa taaagccttg cgaactaggt
     1561 taaaggcatg cataaccgca atgtgacgta catctgctcg gcttctgcct ccaatattaa
     1621 ccttgataac ctcagttcct tgatcttgcg ccaagcctat gaagactgtc ccaactgggt
     1681 gtccttctag gctatctggt cctgccactc cagtcaaact aattccaaaa tcagactggg
     1741 tcttgcttcg tgcctgctca gccatcttct gagctgtaaa ttcagacacc acaccatgtt
     1801 cttccaaatt cttggcagga atatccaaca tccttgattt ttcctccaag ctataggtca
     1861 caaaaccacc cttaaatata cttgaaattc cagaaaaatt cgccacggta gcttggaaaa
     1921 gacctgccgt caaactctct gcagccgcga tggttttccc ttgccttttc agttcttcta
     1981 ccacaatgct ggctaaacta gtttcttccc cataaccata gcaaaagtct cgtaaagaaa
     2041 ttccttcgaa agtctggcag tccaagattt gattttccaa gatatccagc gcttgattcg
     2101 cctcttcttg actgctagcc tttgttgaca gacgtagagt gacttctcct gtcttggcat
     2161 aaggggccaa ggtaggatcg acctgattat caattaaatc agccaaaatc gtaaccagct
     2221 ggctctcgcc aatcccaaag aaacgaagaa ctcgggaata cagcttgctc cctgtcatca
     2281 acttgggtag aagtaggttt aagaccatgg gtttcaattc acttggcgga cctggaagga
     2341 cgacataggt cactccgtct acttctaatt ttcctccaac agccagtcct gtttcgtttg
     2401 gcagtggaat cgctccttct acaatttgag cttgtctttc gttattcggt gttcgggcat
     2461 agtctggtcg cagggcaaaa aagatatcca acttctcctg agcctgagga tcaaagacta
     2521 atgctttccc taaaaattta gctagggttt gtttggttag gtcgtcctca gttggcccca
     2581 aaccgcctgt caaaatcacc agactgctac gttgactggc aatctcaagc aaagacaaga
     2641 gacgaacttc attgtctcct acagccgtct gaaaatatac atctacccca atctcagcta
     2701 gtttttccga caaaaactgg gcattggtgt tgacaatctg tcctgtcaaa atctctgttc
     2761 caacagcaat gatttctgct ttcatgtttc ctcctaccta tctattcgta tttttttgaa
     2821 aaaatcgcag gaattttcct acgattgatt ttttatttgt atcaaaagtt aattatcttc
     2881 atcaccaaca ggtgctctgc caaataaatc ttcaaataaa accgcattgg tttcaagctg
     2941 agtaacttct tcttgtccca aagaacgtcg gagtatattt tgcatttcca acatatgtgc
     3001 tcttgaaaca atctggtaag aaacaccttg aagtatctct ccttcaccct gcaactgctg
     3061 agtttcaatg gttttaaatg aatctttata gcctagcaag ttagggatac tttttgcaga
     3121 caaatcaata ttggtctgca tattgtcact caaagctttt agaatctctt gataatgacc
     3181 aacgctattt aaactgagag ctttttccat gactttttga ataacttcac gttgacgttt
     3241 ttgacgacca taatccccct caggatcttg gtaacgcatt cgtgcataga ctagggcttt
     3301 ttctccccca atatgttgct ccccaacacc gatagaaata gtattaaatt cttcttggtc
     3361 actgatagaa attgggaaac ctaggatatt attgactgta atacctccta ctgcatccac
     3421 tagtttttgc aaccctctca tattgaccat cacatagcga tcaatatgga tattcatcat
     3481 tttttgaatg gtttctatag caagctctgc tccaccatct gcatatgctg agttcagttt
     3541 cgcttcatga gcctgaccat tccctgattc aatgcgcgtc agaatatccc gctctaaact
     3601 catcattgtt gtttttttcg ttttaggatt cactgtcatc aagatcatgc tatcacttct
     3661 accgacccaa gtttcagttc gttcaacatt tccggtgtcc actcccatta acagaatggt
     3721 tagaggttca gtcgcttcaa taaccttggt ttcttcgccg atttttttat aggttttagc
     3781 taaggtttct gtcccttgtt gataaatagt ataagcaaaa acacctactc ctactacagt
     3841 tacagaaagt aaagctagca ccattccaat aattttttta accatatttc tactaaccta
     3901 tcagtttacc catcaagtaa acatcgataa atttcccttc ttctatatat gccccacgct
     3961 cttggctacc ttcaatgaca aagccatgct tttgataaag atggactgct gcttgattac
     4021 gagtttggac agtcagttgg agacgacgca gaatgccact tgcttgtgcc cactctatcg
     4081 cttcttctag caacaaactt cccaagccat tattccaata tctttttcca atcacaatga
     4141 agagatctcc aatatgacgg actctcttac gctgatcagc tgtaatattt acaataccag
     4201 caattttgcc atttaagaat gcaagtaagg ttatctgatt gtccgaacta gcttgcttgt
     4261 tgaggaatat ttccatctcc tcactagtca agagaatacc atctccgtct aggctggtaa
     4321 agtctgtctc caaactcaca cgatttaaaa aggccactaa ttcagctgca tctttgggct
     4381 ctgcttccct aatgagcaat tcatactcca tattgaagct cctctaacaa tttctcagca
     4441 cgcaaaccct ttgcctgaaa atttaaacgg cgtccatctg cttcttttag aatttccaat
     4501 tctaaataag catctggcaa ggcatctcct aagagatttc cccactcaat aacagtcacg
     4561 ccgccaccaa agataaactc atccaagtcg atagaatcag catctccttc aatacgataa
     4621 acatctaggt gataaagtgg aagtcgacct tcatactctc tcacgatagt ataggtggga
     4681 cttttaatca tttgagaaat ctgtaatcct tttgcaagtc ctttagtaaa ggtcgtttta
     4741 cctgcaccca gttctccagt taagattaaa acatcattct ttgctaatag atggcccaaa
     4801 cgctccccta aggcttgcaa ctcttcttca ttttttgtgt acatactctt attataccaa
     4861 aaacttttct tttgtgtcta ttttcctact aaacttatca tcataacatc cataaaaaac
     4921 aggctttctc taaaagaaaa tgagcgtaac aatgaccaat acaagatctc ggaaaatatg
     4981 accataaaag gaaacttcct tcttaaccga atttgggaca agataggctg caaaaaacaa
     5041 gcccagtcca atataaatca gaagtgagac aatggtcatt ggatttctta agaaaagaag
     5101 tgttgctaaa atagtcacca acactgtctt ttttctgtct agcatagcaa gaaaatcgcg
     5161 cacgtatttt ttcaagggta aaaaaatcag caaatctagc ccaaatagga aaaagaagga
     5221 tggcaataaa aagtcaacta attcttgctg cagcgtattt ttgatgaaca agttatctga
     5281 caaaacaaga acagctccta acaaattaat taagagtaac atactgtaaa aaagcttcac
     5341 cgacttctta ctggctagga cactatggac ttcttgctta cgggtataaa gataatttac
     5401 tccagcacag attcctgaaa cgaaaaccat gctcccgatg aaaaaagctg tactttgttt
     5461 aaaggacaag atgcattcct tccataggaa acagctactc aaactgattt gaattaaagc
     5521 taacaaaaat aagattctca ttgatttcat cttctctctc ccttcctacc aatcattata
     5581 ctaggagaaa agagagaact gtttctaatc ttctcaaatg tctctttaag acgctaaaca
     5641 aacactagag actaatactc aatgaaaatc aaagagcaaa ctaggtagct agccacaggt
     5701 tgctcaaaac actgttttga gattgcagat agagctgacg tgatttgaag agattttcga
     5761 agaatataaa tttgaaatca tgaaaatccg tcaaacgggt ggttgttttg tctcgcacct
     5821 cacggagcga gacggactca gagtcacata attataaggc tgatagtatt aatctaacta
     5881 tcagccttac aggttattta acgtttcaga aaaactataa tgtcaagatt aactaaacag
     5941 tatctaattc cttcaaataa ttttctatct tcatcagcat taaaggattg ttataaatct
     6001 tgcatagctc tcttgcttct atataataat ttttgacttg ttctctgtct agaaatttgg
     6061 ctccagcatt tcctacaaga ataagtagag gagccaattg gtagcttgtc tgtctttgtt
     6121 tacagagttc gatagtctca agagcttctt ggatggcttc attatatttt tcctttgata
     6181 ctaggtagtg agcgtaattg taacgaactc tgatgtagcc aaataaaaac tcttgatgct
     6241 caaaattttt tgtctgatat aactccatca aatgagagta gtttacctca tattcttgtt
     6301 cacgacccac taaggaatag aaattagata gagtattcaa cgcctttaaa taaatcaaag
     6361 tatttgagga gacttttaat aatatatttt ccaatgaaga aattgcttca cacttactgt
     6421 catattgaca gaagtcaata atagctttaa tccattcaag gtaagttcgg tcttctagtg
     6481 ttagaaaagt acttcgttca atctctattc tataaatata ttccaaattt tcataatttc
     6541 tatcatctaa taggcgagca gataattgct tgaaattaga gaggttagat ttaatttcaa
     6601 tttgttcatt aaaaaaataa tctaatggca cttcgagtcg ttgtgagagt ttgaacaaaa
     6661 ggtctgcgga gggaatgaaa taccctctct caattttact aatctggctc tattcacaaa
     6721 ttccttctgc aagagtttgt tgggagagtc tcaactcttt tcttttcaat ctgaatttct
     6781 ctgcgagtgt attcattaaa gataataaac cttcctattt gcttaatttc attataaaat
     6841 ttttagttca aaatagcaag ttaaaggaat aaataattct ttgcggtaat aaacaaaaaa
     6901 aagtcaaaaa ttacataaaa atgcttggct aaataaaata tatttgttaa aataaggata
     6961 atgaaaaaga aaagggtttc acgaatgaag aaaaatcgtg gaattcaaaa attagctata
     7021 ttagtattac taggtgtttt tatgtttagt aacacaattc cttaccaaca gtttattcag
     7081 aagaatagac aattggagat tcgagtccaa tcgcaaaaga agtccaatgg tcttgatgtt
     7141 gggaaggctg attgatttta tattacttta aaattcaact atttagagaa aggtattctg
     7201 atgagagctt gtaacaatac cagatagata taagaatgga gatgtttgat aaaatgcttt
     7261 catagctaac caactgttga aaattatgag ttacaaaatc taaagtattt aagattggtt
     7321 tataaaatta ggtctctgcc tgtagaaagg aacgtgcagt tagaaaggag gaaaaaatag
     7381 gatactataa ttcagactga gatttctaat taaaatatat agttgcattt cgctcatttt
     7441 cataatattt cattcagagg agtaataata tgttaaaatt tagaaaagta gttgcatcat
     7501 cccttgccgt tttggcattt agtttggctc ttcctgtttt ggcagtttct caccgtggtg
     7561 gcgagtggac ctatggaggc catcatgatc caaataattg gggagcattt tcgaattatt
     7621 atcatggttc acagtatcac tgggcctatg ttggaagtaa tggacgtaac aatcaaaaaa
     7681 cagtttatgc aggagcacgt agtgcggcct atgcgtttgt taatacaaat tttggggagc
     7741 aagttacatt tgatgcggga tggtaaagaa taattgaaga agattctttt atcaatagtt
     7801 cagtaagctt gttagtttat agagggcagg ggtatttctg ccctctgttt tagaataagg
     7861 aatgcactat gaaaaaatta tttatactca tatccaacct gcttgctagt ttgttctttg
     7921 tttgggtatt taccatttgg actgatacat atgtctccta ttattacccc aacgttgttg
     7981 tacatgattc ttcccctgaa acaacttttc agcatgttgc tacacgcttg gaaaaactag
     8041 cagaagaaac agatagcttt attgcgattc aacaccaaga tcctaactca gaaggtacta
     8101 cagttttttc ttatacgacc tttggggacg gaaagttgcc tgatgggctt caggaaaaaa
     8161 aactagaaga tgctcaaagt agtagtgttg aaacaaacta ttttgtattc gatggacacc
     8221 tagatattca cttgctaagg gaggagctaa gtcaacttgg cttgactaac atgaacctga
     8281 caattccatc taaactatct acgttaatgg ctatctttag taacggattt cagttaatca
     8341 gtctattgat tttcatctta acctttatag cactgacttt aattagtcaa attagccaat
     8401 tacggtcgtc gggcattcgc ctcatttcag gagagaaacg gtggtccatt ttccttagac
     8461 cagttggtga ggatttgaaa gggatagctg ttggtttcag tttagctggc gtgctcgcca
     8521 ttcttatgca aaaaattctg tcgcttccaa cgcaatccct aatgaccata ggagcaggct
     8581 tgctcagtta taatctcatc ttgttgtcga tctccctgtt tttcgctcaa ctcttcgcag
     8641 ttgggataaa aaagatacac ctgatgcaga ttataaaagg gcaagtgcct gtcagaggaa
     8701 tcattagttt gattttaatc ggtcaactat tagcgattat tattgtaacg ctgggaatag
     8761 ggagtagctt aaagtattcc caagcctggc aacagcatcg gattggacaa gaggcttgga
     8821 gtcaggaaag gcaactgatt accctatcaa tcagccgtga aggaacgagt cctggttttg
     8881 atgaacaagc tcaaaggaaa ctcagaactt ggtatcaatt gatggatctg gctgtttcag
     8941 aacaaaaggc tttcttatct agacaccagt taattgaccg tactttgcaa aatggcatgg
     9001 cttcctccaa aaacttgata acctctacag aatggcacga ttacaacccg aatggcaatg
     9061 tccttatcgt cacaccgcaa tacttggagc gtcaaaacat tcctgtagat acaactattg
     9121 agcaaaagat gaatcacctt gatgtagggg agtttgtctt attgctgcct gaacacctcc
     9181 gttcagagga agaacattat aaatctgttt ttgaagacga cttaaccagt cgcatgtcta
     9241 gtcaagatga acgacagcaa atgactgcta cggtaggtta tttagaatca ggtcaggatc
     9301 gttttgtgta taatacgacc cctatttctt accagcagtt tttgaaagat ccaatcatca
     9361 ttgttataac accccaatca actggtccac agtccatttt gttttggata gacgcagtac
     9421 agaactacgt tctctttaat caattgtctg atgcccagga gcttatccag agacaaggca
     9481 ttgaaaattg ggtctcagaa atgcaaacag gttaccacaa ctacatcaca ttattggata
     9541 atattcagag ggaacgttgg gtaatgctag caggagctgg gcttgggatt gcaacttcaa
     9601 tcttgttgtt taacactatg aataggctct actttgaaga atttagacgt gccattttta
     9661 tcaaacgcat tgcaggtctc aggttcttag aaatccatcg cacttatctc tttgctcaac
     9721 tgggtgtgtt tttactggga tttgttgcga gtgtatttct tcaggtagag ataggagttg
     9781 ctttcttagt cttgttactc tttactggtc tatctctttt acagttacat gtccaaatgc
     9841 agaaagaaaa caagatgtcc atgcttgttt tgaagggagg ttaatatgat tgaacttaaa
     9901 caggtgagta aatcttttgg agaacgagag ttattttcga atctttcaat gacatttgag
     9961 gctggaaaag tctatgcctt aattggttca agtggtagcg gaaaaacaac cttgatgaac
    10021 atgattggga aattagaacc ttatgatggg acgatttttt accgaggtaa agacttggcc
    10081 aattataaat cgagtgattt tttccgtcac gaattgggct acctcttcca gaactttggc
    10141 ttaattgaaa accaaagtat tgaagaaaac cttaagctag gtctcattgg tcaaaagttg
    10201 agtcggtcgg aacagcggtt gaggcagaag caggctttag aacaggtcgg cctgacttat
    10261 cttgacctag ataagcgcat ctttgagtta tcgggcggag aatcgcaacg ggttgccttg
    10321 gcaaaagtta tcttaaagaa tccacccttt attctggcag atgagccaac agcttcaata
    10381 gacccagcaa cttctcagtt gattatggag attctgctat ctcttcgaga tgataatagg
    10441 ctaatcatta tcgcaacaca taatccagca atttgggaga tggctgatga agtgttcacg
    10501 atggatcgtc tgaaataaaa atccttgttt ttaattgcac gatgagttac tgaaatatta
    10561 tcatgaatca ataattggag ttaatttaga attgtacttt attaatattg aggtaacttt
    10621 ttcttgataa aggaagaaat agtggagagg aagttagaat gaaaaaattc gacaattata
    10681 ttgttgagaa gccttgcgat tctaattcag ataaactgca aaaaatctta ataattgaaa
    10741 atttggtaga tgatattttg caatttcctc tcagaatcaa taatagtgta ggagagattt
    10801 tcctcctaca accgtttcaa aagaaaacta tctttattcc atgttatttt gaggaagata
    10861 ttgtgaaagt caaagatgat gataaagttg agtggaattt gttagaattt caaaaattta
    10921 gagcattttt ggcttagtaa tctgtgttga aggctcaaaa cctatggtaa aaaagtcgct
    10981 ttgaaaacgt attgcctcca aagatttagt taaataatga tttaacacaa aaagaaatta
    11041 ttgaagttct ggaaagatgt tgtttcagta ttgagaaaag gtgggaaaaa cttgcgattt
    11101 tcacagagaa aggaagaaaa agtatagaaa tatagtcaat tgaaacaaga acaggacaaa
    11161 agaacctttt gtgccatatt tttctccttt cgctttacaa ttgg
//
LOCUS       DAVBHS010000060        11149 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVBHS010000060 DAVBHS010000000
VERSION     DAVBHS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11149
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.060"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..330)
                     /locus_tag="VQ651_001129"
     CDS             complement(<1..330)
                     /locus_tag="VQ651_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2754136.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSK"
     gene            complement(396..620)
                     /locus_tag="VQ651_001130"
     CDS             complement(396..620)
                     /locus_tag="VQ651_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HET2754137.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(698..1441)
                     /locus_tag="VQ651_001131"
     CDS             complement(698..1441)
                     /locus_tag="VQ651_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2754138.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILLLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(1441..2241)
                     /locus_tag="VQ651_001132"
     CDS             complement(1441..2241)
                     /locus_tag="VQ651_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2754139.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(2399..2755)
                     /locus_tag="VQ651_001133"
     CDS             complement(2399..2755)
                     /locus_tag="VQ651_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET2754140.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(2768..3280)
                     /locus_tag="VQ651_001134"
     CDS             complement(2768..3280)
                     /locus_tag="VQ651_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET2754141.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(3280..3774)
                     /locus_tag="VQ651_001135"
     CDS             complement(3280..3774)
                     /locus_tag="VQ651_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2754142.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            3909..4355
                     /locus_tag="VQ651_001136"
     CDS             3909..4355
                     /locus_tag="VQ651_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HET2754143.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            4352..4999
                     /locus_tag="VQ651_001137"
     CDS             4352..4999
                     /locus_tag="VQ651_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET2754144.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(5020..5601)
                     /gene="pdxT"
                     /locus_tag="VQ651_001138"
     CDS             complement(5020..5601)
                     /gene="pdxT"
                     /locus_tag="VQ651_001138"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET2754145.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(5602..6477)
                     /gene="pdxS"
                     /locus_tag="VQ651_001139"
     CDS             complement(5602..6477)
                     /gene="pdxS"
                     /locus_tag="VQ651_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET2754146.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(6629..8008)
                     /locus_tag="VQ651_001140"
     CDS             complement(6629..8008)
                     /locus_tag="VQ651_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2754147.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKDKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            8303..9226
                     /locus_tag="VQ651_001141"
     CDS             8303..9226
                     /locus_tag="VQ651_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET2754148.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGISPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            9286..9891
                     /locus_tag="VQ651_001142"
     CDS             9286..9891
                     /locus_tag="VQ651_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2754149.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKTIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLTHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            9909..>11149
                     /locus_tag="VQ651_001143"
     CDS             9909..>11149
                     /locus_tag="VQ651_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2754150.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRNFKFKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGAS"
BASE COUNT         3269 a         2527 c         2035 g         3318 t
ORIGIN      
        1 tttagaacca gttgctacga taactgtacg tgtttcatat gtttggtcat cagtcatcac
       61 tttcttaaaa tcaccatggt cttcgacatt ttcaacataa ccataaatgt gctcaacacc
      121 aagattttca agtggttcaa acatcttttc agccaattca ggtccactaa tattagcgta
      181 tcctgggtaa ttttcgatat cagatgtatt attcatctga ccacctggca gaccaccttc
      241 aatcaaagct acttttagat tgcttcgagc agcatacaag gccgcagtca tccctgcagg
      301 tccagcaccg ataataatag tatcgtacat atagattcct tctttcttgt tgtaactatc
      361 tttattctaa ctctttcttg tcaatcacgc aaggattatg ccttgaaaac aagaattaaa
      421 ctcataaccg caaaggataa tactgggaca accaagactc caatcataat catatcatag
      481 agatgggctt ggcgagccct ggctgtctta tcaactcccg acatggctct cagaccaatc
      541 caaatcccta aaaaaatcag aacaaggatg gtggtcaaga tcaaactctc gaaatataaa
      601 gaaaatagtt gcagtagcat gatttctctc atttctatct tttttaaaga gtaaactcag
      661 ctagtccaac taactgagtt ttcctttatc tattatatca aatataagtc cgtttgtaac
      721 tagcgaagaa ttcttttgtc cgctcttctt taggggtgtg gataatctca tccggagttc
      781 cagactcgat gattttcccc ttatctaata agagaatttt atccgcaact tgggctacaa
      841 aggacatgtc atgactgacc aaaatcatgg tctgacctga cttagcagca tctgcaatag
      901 acttttctac ttcaccgacc aattctgggt caagggctga agttggttcg tctaagagca
      961 aaacatctgg tttcatagca agcgcacgcg ctagggcaac ccgttgcttc tgtccacctg
     1021 ataaatggcg aggataatgg ttttcacggt ccgaaagccc aaccttagcc aactcttcct
     1081 tggcaatctt agtcgcttct tggtcagata atttcttgac aacaaccaag ccttctttca
     1141 cattatcaag tgctgttcga cgttcaaata aattaaactg ttggaaaacc atagacaact
     1201 tacggcgtag ggcaaggatt tcttcttgag tgattttaga aaaatcaact gaaaaaccat
     1261 caatctgaat agagccactg tcaggtgttt caagataatt gagactgcga agaaaggttg
     1321 attttccagc tcctgaagaa ccaatcaagg ctacaacttc ccctttttga atatccaagt
     1381 tcagatgatc caagacagtc tgtcctgaaa aggatttgct taaattcgaa atcttaatca
     1441 ttaacgaagg tctcctttca catctgtttg cactgtatca ggtgcagaaa tagccatttt
     1501 tctctcgatg aaacgaccga ggctttcaat tccgatattg actacccaat aaacaagggc
     1561 aacagagatg aagcgttcaa aatagcgata atcagctcca cctagaatct gagcttgggc
     1621 aaagacttcc acaacacccg cactaaaagc tagagatgtt cccttggtca aaccgatgag
     1681 ggaattaatc aaggttggag tagctaccac cgctgcatta ggaataatca ctcgtcgata
     1741 aacttgcgct cgggtcatac ccagactgcg cgccgcctca atctcaccag gattaactga
     1801 gagaatggct gcacgaatgg tttcactagc ataagctgcc tcattaaagg caaaagcgac
     1861 aatcgcaaaa gctgcagctg gaatcgcatt gatattgaga ccagttcccc attgctgatt
     1921 gagggctttc aaagccaaag ggattccgta gtaggtcaac atgagttgca ccaaaatcgg
     1981 tgtccctttt aagaaactaa caaagaaggc ctgcaaggga tataaaatct tgacacgatt
     2041 gatcttcaca atggcaaaaa gaagcgccaa aaccaagcca aaaagggcac caccaattgt
     2101 caacataatt gttgttggaa gttgttggac aattctaggg attccatcaa agaccgaacg
     2161 taggctaaac agcttgccat ccggaatcaa ttgcatcaag ttttggtacc aatctgatgc
     2221 taaaatcgtt gtaacattca taaaaacatc ctcttctgat aatgattcat tctaccatgc
     2281 ttctgccgtc aatgaaatta tttgctacgg tcagatacag actttgtcta cctactagtt
     2341 tatcatactt tgaaacaggg aagttatata ttttatctat caaagagata gataaaaact
     2401 atttcagtcc cagttcttca taagattttc gataaccgat ttgcttaaca gttccatttt
     2461 ctactaaaat gggccgtttt aacaacatac cgtcacttgc tagcaactca gccgcttctt
     2521 ggtttgacaa acttcctacc ttatctttta gccctaattc acggtatttg ataccactgg
     2581 tgttgaaaaa ttgcttcaat tcaaatcctg aggtttctag ccaattcaaa atgacttctt
     2641 ggctaggtgt ttcttccacg atatggacgg ctttatagtc cacacctaat tgatttaatt
     2701 cttgttttgc ttttttacaa gttgaacatt tggggtattc gataaattct aacatgtgct
     2761 tctccttcta tttgtttata tctttaaaat ctacgccttc atgctccaag agccaagctt
     2821 tcttttccac tcctgcagca tagcctgtca gacgcttgcc tgctcccaac acacgatgac
     2881 aaggtactag gatagaccaa ggattgcgtc ccactgctcc accaattgct tgagcagaag
     2941 ccacttgcag gtcttgagca atttgtccat aggtcactgt ctgaccataa ggaatgccct
     3001 gtaaatagtc ccaaactctc ttttcaaaat tcgttccgat tggcgccaag agcaagttgg
     3061 ataaatcctg aggcttgcct ttaaagtaat catctaagca agcaataact gggtctaaaa
     3121 taggatgact aacaacttct tctatcgttt catctcctag tcccctctca aaatgcttct
     3181 gctcctgaac ccaaattcca tacaaataat ggtcatcagc tacaagtgat agaataccaa
     3241 ttggtgagga gtagagtatt tttacatact tcttttgcat tatttcttta atttttgata
     3301 ggccaagcgt tcgccatcta ctggcacctt accaacttgt ttaaaaccaa gtttttcaaa
     3361 aatatgttgc ataaccttgt tttcagcatg cgtatctgag cgaaaatcaa gataatcaaa
     3421 accttcaatc aagccctcta agaaggtttg agcaactcct tttccctgca catctgctgc
     3481 cacagcaata cggtgaaaga ctagatactc tgactctcca gcttgccagt ttccctcata
     3541 aatagcttca taggcctcct ctggactctt ggtcacagca gcataggcta gcagttctcc
     3601 ctcttccaag gctacgtagg cttgccctga gatgatatca tcgataataa catctgcatt
     3661 tggatagcca ttttgccact ggtcactacc agcatctgct aaacattttt tggcatcctc
     3721 catcacctgc atgatggcat ctacttcatt tggaaaagct aaacgaattt ccatcttttt
     3781 tcctcatttc tgactagttt ctcccatcat agcataattt aagtatttct acaagcagtt
     3841 aaaacttgac tttgtgtttt tgttatttta taatctagtt atcaaaacta tataaaaagg
     3901 agttgaaaat gaaaactacc ttttcctacc caaaatgggc agaaattcca aacattgacc
     3961 tctatctgga ccaggttttg ctctatgtca atcaggtctg cgcccctatc tctcctaata
     4021 aagacaaggg cctaacagca tctatggtca acaattatgt gaaaaatggt tacctgacaa
     4081 agcctgacaa aaaaaaatac caacgccaac agattgcccg tttgattgct atcacaaccc
     4141 tcaagtctgt attttctatt caagaaatag cccagacact taatactcta caaactcaag
     4201 caagttcaga ccaactctac gatgcttttg tggactacat gaaccaagga attgacccag
     4261 ctaaccctat tatccaaacc agctgccaaa ccgtcaaact ctatcatcaa actctagact
     4321 taatcgatca tactcaagag gaggtaatcc aatgaacact agtcttaaac tcagcaaaca
     4381 actcagtttt ggagaggaga ttgctaatag cgtgacccat gctgtgggtg cagtcatcat
     4441 gcttatcttg ctgcctattt catccatcta tagttatgaa gcacacggat ttttatcctc
     4501 tatcggcgtt tccattttcg tcatcagtct ctttctcatg ttcctatcat ccaccattta
     4561 tcactctatg gcctatggtt cgacccacaa atatgttttg cgaatcattg accattctat
     4621 gatttacgtt gccattgccg gctcatacac gcccgttgtc ttgaccttga tgaataactg
     4681 gtttggctat ctgattattg tcatccaatg gggaacgacc atctttggta ttctctataa
     4741 aatctttgct aaaaaggtca atgagaaatt tagccttgct ctttacctga ttatgggctg
     4801 gttggttctg gctatcattc ctgccattat cagtcaaacg acacccgttt tctggagtct
     4861 catggtaact ggcggactct gttatacagt tggagctgga ttttatgcca agaaaaaacc
     4921 ttatttccac atgatttggc atctctttat cctagctgcg tccgcacttc aatacatcgc
     4981 tattgtttat tacatgtaaa aaagttgaga aattcaatct caactttttt ctttacacat
     5041 attgataaag tactggtgca agcgcacatc atcagtcaat tctggatgaa aagaacttac
     5101 caacatattt ttttcttggg ctgcaacaat ttgattgttc actgttgcta gaatttctac
     5161 accctcacca acactactga taatcggacc acggataaag gtcattggaa tcttgccaac
     5221 tcccttacat tctgcttccg tgtagaaact tcctaattgg cgcccataag cattacgctc
     5281 gaccaccata tccatagttc ctagatgact ctctttctga gaagtgattt ccttagccag
     5341 caaaattaag cccgcacagg tcccaaacac tggtaagcca gatagaatgg cttctcggat
     5401 gggaagtagc atgttctggt cacgtaagag cttgcccatg gttgtagact caccaccagg
     5461 caaaatcaaa cccgacaagt cactctgatc ttgctgaaaa tcatctagat ttctgagttc
     5521 tacactctcg acacctaatt gatctagcac ttttgcatgt tctgcaaagg ccccttgcaa
     5581 ggccaatatt ccaattttca tctattttcc tcgttcagcc atgagaattt ggatttcatt
     5641 ttcattaata ccaaccatgg cttctcctaa atcttcagag atttgagcta ggatttgagg
     5701 attacggaag ttagtcacag ccttaacaat ggcactcgct cgtttaacag gatctcctga
     5761 cttgaaaata cctgaaccga caaagacccc ctctgcccct aattgcatca ttaacgcagc
     5821 atctgctggc gttgcaacac ctccagcagc gaaatttaca actggcaatt ttccatgttc
     5881 atgaacatat tggaccaatt ctacagggac ttgcaaatcc ttggcagcaa cataaagctc
     5941 gtcctcacgt aagttttgaa tgcggcgaat ttcctgattc atcatacgca tatgacgaac
     6001 agcttggacg atatcccctg tccctggttc tcctttggta cgaatcatgg aagcaccttc
     6061 agcgatacga cgcaaggctt cacccaaatc cttagcacca cagacaaaag gaacttggaa
     6121 ttctttcttg tccacatgga aacggtcatc agctggagat agaacttcac tctcgtcgat
     6181 ataatcaatt tcaatagcct ctaaaatctg agcttcaaca aaatgcccga ttctgacctt
     6241 agccattact ggaatactaa ccgcttcttg gatttcctta atcatctttg ggtcgctcat
     6301 gcgggaaact cctccagctg cacgaatatc agccggaatt cgttccaagg ccatcacagc
     6361 tgccgcacca gcagcttctg cgatacgagc ctgttcagga ttctgcacat ccataataac
     6421 accacccttg agcatctgtg ccaagttttt atttagttca taacgatttt cagtcatttg
     6481 ttttatcctc atcatttgta ttggtagcta ccttttcatt ttaagtcaga acagaatgaa
     6541 gcacaagata aaaaaactat aattgtatgg ggacagattg gctaaaaact atctgacctt
     6601 aacttaaggc cagatagctc attcattttt atttttcagc cgtaagggca gccattgtga
     6661 tgtagttgta tggtttattg aagtgtggca agaagaagag gtctgtcaat gccaatttat
     6721 caattgtcac atgctcttgg atagcaagtg agaacatgtg gattcccatg ctaattgcaa
     6781 tatcatgtga aaccatttgg gcaccaagaa tttcacggct atctttgtca aagacaatct
     6841 taattgctac ttcatggttg tcatgtttca tgaattctgg tttttgaaga tcgttaaagc
     6901 ctgtttcagt tgcgttgtaa ccagcagctt tcgctttttc aagagtcaaa ccagttgaaa
     6961 ccatgtgaag accgtagatt gagataccat ttgaaccttg aacaccgatt ccttccaatt
     7021 catgtccaca agcgttgtag gcaccaacga taccagtgcg aacagcattt gaagcaagag
     7081 cgatatagct tgtatcttta cgagcattgt cataaacagt cgcacagtca ccaacagcgt
     7141 aaacgcctgg gatagatgtt tcttgtttct tgtctacaag gaaggcaccg ttgcggaaga
     7201 gttcgatctt accatcagca agggctgtgt ttggacggaa accaactgca aggataacca
     7261 tatccacgtc aaagctttct ttgtcagtaa tcaagcgttc aactttacca tcaccttcga
     7321 ttgctttaac agtttgacct agagccaagc ggatgttgtg atcttccaag ttcttcgcca
     7381 tcatttgtgt gaagtctttg tcatagtaac cgttcaagac agtatcaacg atatcaacaa
     7441 ggacaacttc ttttccaaga cgttcaaagg cttcagcaag ttcaacaccg atgtaaccac
     7501 caccaacaac ggcgatacgg tcgagatgtt ggctcttgtc agaaagttta ttgataactt
     7561 cttcagcatt ttggtacaat ttcacgaatt gtacgttttc aagagttgct ttaaattcgc
     7621 ggtttccttt aacaatttca acaccttcga ttggtggcaa gattggtgta gaacctgtag
     7681 cgaaaatcaa tttctcgtat gattctttgt gttcttttcc ttcaacttct gctgtaacca
     7741 ctttgttatc atagtcgatt gaaagaactg gtgaattcat ataaacctta gcacctttag
     7801 cttctaattt gtctttatca gaatagaaca agccttcagc accgtcaatt tgttcaccaa
     7861 tccaaagagc cattccacat cctaggaaag agatgttaga gttttggtca aatacaacaa
     7921 tttcgttctc atttccaaaa ttatccaaca tggtattgat acatgctgta ccagcgtggt
     7981 tagcaccgac tacaacgatt ttactcataa aaaatttcct gccttcattc taaatttact
     8041 ttactagtat accatttacc gttagcgttt acaagggggg gactattatt tcagcatttt
     8101 ttatcaaaaa taattcccca tttatcctgt tttcaagcct catgaatttt atgagcatat
     8161 ttcttgactt tttccaaatt ttgtttgtga aaattctaac tttatggtat tataataaca
     8221 tgaaaataaa atgtaagggc ttcaatttag atgattattt gatattgcaa cttattttct
     8281 ataagagaaa ggagttggta gcttgactct tcgttcacat tctgaacggc taatggggac
     8341 tactatcact atttcattag tggatgagca agccgatatc tttctccaaa aatcctttga
     8401 cttgctcaaa gagcttgaat accgcttcaa cgccaatagt caagaatctg agttgatgga
     8461 aatcaattat caagcaggaa tatcccccgt cacagttcat ccagacctgt ttgagctgat
     8521 ttcacttggg ttagagcata gcctagcgct ctctagccac ctcaatatca gcattggtcc
     8581 cttaattcaa acctggcgta tcggtttttc agatgccaag gtcgcccagc ctcaagaaat
     8641 tgaatcggtg ctgcctctaa tcaatcctca tggtatcgag ttagattctt ctacttctac
     8701 tgtgttttta aaacagaaag gaatgaagat cgatctaggt tgtttagcca aaggatacag
     8761 tgcggataag gttgcccaat ttcttaggaa agagggggtg acttctgcct tgatcaatct
     8821 gggagggaat atcctgacca ttggaaaaaa tcaggcaaga ggggataacc catggcaaat
     8881 cgggattcaa gacccagcca atcctagggg aaatcattta atgaccatcc ctgttgtcaa
     8941 taaatctgtc gtgacttcag gcatttatga acgtcacctg accgtcgatg gacaagatta
     9001 ccatcacatt tttgacagtc aaacaggata tcctgttgaa acggaactag cgagtctaac
     9061 aatcatctct gataaatcag tcgatggcga aatctggacg actcgactct ttggagaaag
     9121 acctgcttct atcctctggc aagtcgaaag tttggagggc atcgaagtca tcctcatcga
     9181 taaagaaggc cacctaagct gttcttcagg aattcctact ctatagagac aactgtttca
     9241 atggaatttt aaaattgtat tatgtaaaaa gagaaaggaa atatcatgtt aaaacttatt
     9301 gctattgttg gaacaaattc aaaacgttct acaaaccgtc aattgcttca atacatgcaa
     9361 aaacactttg ctgacaaagc tgaaattgaa cttgttgaaa tcaagaccat tcctgtcttc
     9421 aacaaaccag ctgacaagca agtacctgct gaaatattgg aaattgctgc taaaatcgaa
     9481 gaggcagatg gcgttattat cggtactcct gagtatgatc actctattcc agctgttttg
     9541 atgagcgctc ttgcttggtt gtcttatggt atttacccac ttttgaacaa accaatcatg
     9601 attacaggtg cttcttacgg tacgcttggt tcatctcgtg cccaattgca acttcgtcaa
     9661 atcttgaatg ctcctgaaat caaggcaaat gttcttccag atgaattctt gctcacacac
     9721 tctcttcaag catttaaccc aagtggcgac ttggttgacc ttgatgttat caagaaattg
     9781 gatgccatct ttgatgactt ccgtatcttc gtgaagatta ctgagaaatt gcgcaatgcg
     9841 caagaattgc ttcgcaaaga tgctgaagac tttgactggg aaaatttgta agataggaga
     9901 ccgaaagaat gaaatttgtt ggacttgttg gatcaaacta cgatcaatca tataaccgca
     9961 aactcttgga atttatccgt cgcaatttca aattcaaatt tgaattagaa gtacttgaaa
    10021 tcgacgaagt tccaatgttt aaccaagacg aaaaatggga cgaaagcttc caattgcgtt
    10081 tcttgtataa caagattaca cgtgctgatg gtgtcattat cgccactcct gagcacaacc
    10141 acactatctc agcttcactc aaatctgtac ttgaatggct ttcatacgaa gttcatccat
    10201 ttgaaaacaa gcctgttatg attgtgggtg catcatacta tgaccaagga acatcacgtg
    10261 ctcaagttca ccttcgtaaa atccttgatg ctccaggtgt taatgcctac acgcttccag
    10321 gtaacgaatt ccttcttggt aaagctaagg aagcttttga taacaacgga aatatcacca
    10381 acgaaggcac tgttaaattc cttgaaactt gcttagataa ctttgttaaa tacgtaggag
    10441 tcgtttcgaa attgaaaaaa ccaaaaccaa tcgaatcaga agacttggat tgtggaaaac
    10501 caatcgctac aaccatcaca gaagttgacc ctgacgatcc agaatgggta gaaaaagttg
    10561 cagcaatcac tggtgctgtt tctggtgata cctatgtcaa attggaccac ggtatcctaa
    10621 cagttaacca aattgatatg ttcttgaaag ctatgccatt tgaattgaca tacgctgacg
    10681 acaacaacca attcctctac tacaacaacg ctcaccaaga tccagacact atgttcgcta
    10741 aacgtgtacc acctcaatca ggtagccgta tgtcgactgt tcatggttct cttccaccag
    10801 cacgtatgaa gaacgtagag tgggttatcg gaacacttcg caacggaaac caagaatacg
    10861 tccgtacgat cgttccaggt tctcctgcag gtgttatcaa cactcacaac taccaagcaa
    10921 tgtactatcc tgatggatca tacgctggta tcaatgaaat cgtctttaac ttccaaccat
    10981 ggcttgactg gtacctaaaa gagactggtc aacgtttggt gggtggtagc ggaccatttg
    11041 ctcctgctgc cggaggtcat ggagacgccg atgctacttc tggcgcttct gattcagggg
    11101 atgctggagg ccacggtggt gacgcagacg ctacagccgg tgcaagtta
//
LOCUS       DAVBHS010000061        11113 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVBHS010000061 DAVBHS010000000
VERSION     DAVBHS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11113
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.061"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            86..2308
                     /gene="recJ"
                     /locus_tag="VQ651_001144"
     CDS             86..2308
                     /gene="recJ"
                     /locus_tag="VQ651_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET2754151.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKTYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2483..4144
                     /locus_tag="VQ651_001145"
     CDS             2483..4144
                     /locus_tag="VQ651_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET2754152.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPEHIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKILSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4213..4992
                     /locus_tag="VQ651_001146"
     CDS             4213..4992
                     /locus_tag="VQ651_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HET2754153.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5104..6324
                     /gene="murM"
                     /locus_tag="VQ651_001147"
     CDS             5104..6324
                     /gene="murM"
                     /locus_tag="VQ651_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HET2754154.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6329..7561
                     /gene="murN"
                     /locus_tag="VQ651_001148"
     CDS             6329..7561
                     /gene="murN"
                     /locus_tag="VQ651_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HET2754155.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7640..8341
                     /locus_tag="VQ651_001149"
     CDS             7640..8341
                     /locus_tag="VQ651_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET2754156.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKMGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8381..10228
                     /gene="uvrC"
                     /locus_tag="VQ651_001150"
     CDS             8381..10228
                     /gene="uvrC"
                     /locus_tag="VQ651_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET2754157.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGIHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVAAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQRKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            10218..11051
                     /locus_tag="VQ651_001151"
     CDS             10218..11051
                     /locus_tag="VQ651_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET2754158.1"
                     /translation="MNHKIAILSDIHGNATALEAVIADAKTQGASEYWLMGDIFLPGS
                     GANDLVALLKELPITASVRGNWNDCVLEALDGQYGLEDPQEIQLMRMTQFLMERMDPA
                     TTVWLRSLPLLEKKEIAGLRFSISHNLPNKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
BASE COUNT         3346 a         1947 c         2656 g         3164 t
ORIGIN      
        1 ttataaaaaa gcaatctgga tagagaaaac gtctaaatca tgttataatg aagcaataga
       61 attcttagaa agagtggatg tctttttgat aacacctact tatgaatggc agtttgccct
      121 gcaggtagaa gatgcggatt ttacaaagat agccaagaag gctggactgg gtcctgaggt
      181 ggctcggtta ttgtttgaga gagggattca ggaccaagaa agtctgaaga agtttttaga
      241 accttccttg gaggacttac atgatgctta tctgctccat gatatggaca aggcagtgga
      301 gcggattcgt caggctattg aagaagggga aaatattctc gtttatggag actatgatgc
      361 ggatggcatg acttcggctt ctattgtgaa ggaaagtttg gaacaacttg gtgctgagtg
      421 ccgagtttac ctgccaaatc gttttaccga tggctatggc cctaatgcta gtgtttataa
      481 atactttatc gagcaagaag gaatttcctt gattgtgacg gtggacaatg gggttgctgg
      541 tcatgaggct attgcattgg ctcagtctat gggagtagat gtcattgtga cagaccatca
      601 ttccatgcct gaaaccctgc cagatgctta tgctattgtc catcctgaac atccagatgc
      661 ggattatcct tttaaatatt tggctggttg tggagttgct ttcaagttgg cttgtgccct
      721 gttagaagaa gtgcaagtgg aattgcttga tttggtcgct attggaacta ttgcagatat
      781 ggtgagtctg acggatgaaa atcgtatctt agttcaatat ggtctggaaa tgttgggtca
      841 tacccagcgc attggtctgc aagaaatgct ggacatggct gggattgctg ccaacgaagt
      901 aacagaagaa acggttggtt tccagattgc tcctcgtttg aatgccttgg gccgcttgga
      961 tgatcccaat cctgccattg atttgttgac tggatttgat gatgaggaag cgcatgagat
     1021 tgcccttatg attcaccaga aaaacgaaga gcgcaaggaa atcgttcagt ctatctatga
     1081 agaagccaag accatggtgg atcctgagaa gaaggttcag gtcttggcca aggaaggctg
     1141 gaatcctggg gttctaggaa tcgtggctgg tcgtttattg gaagaattgg gacagacagt
     1201 cattgttctt aatatagaag acggtcgtgc caagggcagt gctcgtagtg tggaagcggt
     1261 cgatattttt gaagctctgg atccccatcg agacctcttc atcgcctttg gaggtcatgc
     1321 aggtgcagcg ggtatgacgc tggaagttga gcaactctca gatttatctc aggttttgga
     1381 agattatgtt cgtgaaaaag gtgcagatgc tggtggcaag aataagttaa acctagatga
     1441 agagttggat ttggaggcac ttagcttgga aacggtcaaa agttttgaac gtttagctcc
     1501 ttttggaatg gataatcaga aacctatttt ttatatcaag aattttcagg tcgaaagtgc
     1561 tcgtactatg ggggcaggta atgcccatct aaagctgaaa atttccaagg gtgaggcgag
     1621 ttttgaagtg gtagcctttg gtcaaggcag atgggcgaca gagttttctc aaaccaagaa
     1681 tctagagtta gcggttaaat tgtctgtcaa ccaatggaat ggccaaactg ccctccagtt
     1741 gatgatggtg gatgcgcgag tggaaggtgt tcaacttttt aacattcgtg gaaaaaatgc
     1801 agtcttgcca gaaggtgttc cagtcttgga ttttcctgga gaactgccaa atcttgcggc
     1861 tagtgaagct gttgtcgtaa aaaacattcc agaggatatt actcagctga agaccatttt
     1921 tcaggaacag catttctctg ctgtctattt caaaaatgat attgacaaga cctattatct
     1981 gacaggttat gggactagag atcagtttgc taaattgtac aagactattt accagttccc
     2041 agagtttgat attcgctaca agctgaaaga tttggctgca tatcttaata ttcaacaaat
     2101 cttgctggtc aagatgattc aagtatttga agaactaggc tttgtgacga taaaagatgg
     2161 tgtgatgaca gtcaataaag aggcgccaaa gcgggagata ggagaaagtc aaatttacca
     2221 aaatctcaaa caaaccgtta aagaccaaga aatgatggcg ctgggtacgg tgcaagaaat
     2281 ttatgatttt ttgatggaaa aagagtagaa gttaggaaag agttgggaaa tcaactcttt
     2341 tttgaaaaca gaccttcatt ttgaaaatca tcaaaaaaat ggtataatgg taggaaaaga
     2401 ttcggctgaa agtatcagaa cttttagaat aagagggtag aattgcccta taatcaagat
     2461 aaactaagat tttggaggaa aaatgagtaa tatcagttta acaacacttg gtggtgtgcg
     2521 tgagaatgga aaaaatatgt acattgctga aattggagag tccatttttg ttttgaatgt
     2581 agggttaaaa tatcctgaaa atgaacaatt aggggtcgat gtggtgattc caaacatgga
     2641 ttaccttttt gaaaatagcg accgtattgc tggggttttc ttgacccacg ggcatgcgga
     2701 tgccattggt gctctacctt atctcttggc agaggctaaa gttcctgtat ttgggtctga
     2761 gttgaccatt gagttggcaa agctctttgt caaaggaaat gatgccgtta agaaatttaa
     2821 tgatttccat gtcattgatg agaatacgga gattgatttt ggtgggacag tggtttcctt
     2881 cttccctacg acttactccg ttccagagag tctgggaatt gtcttgaaga catcggaagg
     2941 aagcatcgtt tatacaggtg acttcaaatt tgaccaaacg gctagtgaat cttatgcaac
     3001 tgattttgct cgtttggcag agattggtcg tgacggcgtc ctggctctcc tcagtgattc
     3061 ggccaatgca gacagcaata ttcaggtggc tagtgaaagt gaagttaggg atgaaattac
     3121 ccaaactatt gctgactggg aaggtcgtat catcgttgca gctgtttcca gtaatctttc
     3181 tcgtattcag cagatttttg acgctgcgga taaaacaggt cgacgtatcg tcttgacagg
     3241 atttgatatt gaaaatatcg tccgcacagc gattcgtctt aagaagttgt ctttagccaa
     3301 cgaaattctc ttgattaagc ctaaagatat gtctcgcttt gaagaccatg agttgattat
     3361 tcttgagaca ggtcgtatgg gtgaacctat caatggactt cgtaagatgt cgattggtcg
     3421 ccatcgttat gtagaaatca aggatgggga cctggtctat attgctacgg ctccgtctat
     3481 tgctaaagaa gcctttgttg cgcgtgtgga aaatatgatt tatcaggcag gtggggttgt
     3541 caaattgatt acccaaagtt tacatgtatc agggcacgga aatgtgcgtg atttgcagct
     3601 gatgatcaat cttttgcaac ctaagtacct cttccctgtc caaggggagt atcgtgagtt
     3661 ggatgctcac gctaaggctg ccatggcagt tgggatgttg ccagaacaca tcttcattcc
     3721 taaaaagggg acgaccatgg cttacgagaa tggagacttt gttccagctg gatcggtttc
     3781 agcaggagat atcttgattg atgggaatgc cattggtgat gttggaaatg ttgttcttcg
     3841 tgaccgtaag atcttgtcag aggatggaat tttcatcgtg gctattacag tcaaccgtcg
     3901 tgagaagaaa attgtggcta gggctcgtgt tcatacgcgc ggatttgttt atctcaagaa
     3961 gagtcgcgat attctccgtg aaagttcaga attgattaac caaacggtag aagattatct
     4021 tcaaggagat gactttgact gggcagatct taaagggaag gttcgagata atttgaccaa
     4081 gtatctcttt gaccaaacca agcgtcgtcc agctatttta ccagtagtca tggaagcaaa
     4141 ataatcgttg aaataaacag agagaaagtc gagtttcggc tttttcttat agaaaaatag
     4201 aaggagaaaa tcatggcagt aatgaaaatc gagtattact cacaagtatt ggatatggag
     4261 tggggggtga atgtcctcta ccctgatgcc aatcgagtgg aagaaccaga gtgtgaagat
     4321 attcccgtct tgtacctttt gcacgggatg tctggaaatc ataatagttg gcttaagcgg
     4381 accaatgtag aacgcttgct tcgaggaact aatctcatcg ttgttatgcc caataccagc
     4441 aatggttggt acaccgatac ccagtatggt tttgactact acacggctct agcagaggaa
     4501 ttgccacagg ttctgaaacg cttcttccct aatatgacta gcaagcgtga aaagaccttt
     4561 atcgctggtc tttctatggg aggttacggc tgcttcaaac tggctcttgc gacaaatcgt
     4621 ttttctcatg cagctagttt ttcaggtgcc ctcagctttc aaaacttttc tcctgaaagt
     4681 caaaatctgg gaagtccagc ctactggaga ggtgtttttg gagagattag agactggaca
     4741 actagtccct attctcttga aagtctggct aaaaaatcgg ataaaaagac caaactttgg
     4801 gcgtggtgtg gcgaacagga tttcttgtac gaagccaata atctcgcagt gaaaaatctc
     4861 aaaaaactag gttttgatgt gacctatagc catagcgctg gaactcacga gtggtactac
     4921 tgggaaaaac aattggaagt ttttttaaca accctaccaa ttgatttcaa attagaagag
     4981 agactgactt agtttgaact tcagcatagg gggagtagaa ctaaaataaa atatgttttc
     5041 actagacttt tcaaacgaaa gtagtagaat agtaataaaa tactggagga aagagagtag
     5101 gaaatgtacc gttatcaaat tggcattccc acattagaat atgatcagtt tgtcaaagaa
     5161 catgaattag ccaatgtatt acaaagtagt gcttgggagg aagttaagtc taattggcaa
     5221 catgagaagt ttggtgttta cagggaagaa aaattactgg cgacagctag tattttgatt
     5281 agaactcttc cgctaggcta taaaatgttt tacatcccaa gaggacctat attggattat
     5341 ggggataaag aactcttgaa ttttgccatt cagtctatta agtcctatgc tcgcagtaag
     5401 agagcggttt ttgtgacttt tgacccaagt atttgcctat ctcaaagttt aatcaatcag
     5461 gaaaagacag aatttcctga aaatctggct attattgata gtttgcaaca aatgggagta
     5521 aggtggtcag gaaaaacgga ggaaatggga gacaccattc aacctcgtat tcaggcgaaa
     5581 atatacaagg aaaattttga agaagataaa ctttccaagt caacaaaaca ggctattcga
     5641 acagcacgaa acaaagggct tgagattcaa tatggtggac tggaactatt agattcattt
     5701 tcggagttga tgaaaaaaac tgagaagcga aaagagattc atttgaggaa tgaagcctat
     5761 tataaaaaat tgttagataa ttttaaggac aaggcctata tcaccttggc caccttggat
     5821 gtttctaaac gttcgcaaga gttagaagaa cagttagcga aaaatagagc cttggaagag
     5881 acctttactg agtcgactcg aacttcaaaa gtagaagcgc agaagaagga aaaagaacgt
     5941 ttgttagagg aattgacctt cttgcaggaa tatatagatg taggtcaagc gagagttcct
     6001 ttagcggcta ctttgagttt ggaatttggt actacctctg tcaatatata tgctggtatg
     6061 gatgatgatt ttaaacgtta caatgcacca attttaacat ggtatgaaac ggctcgctat
     6121 gcctttgagc gaggtatggt ctggcaaaat ttaggtggtg ttgaaaactc tctcaatggt
     6181 ggactttatc attttaagga aaaatttaat ccaacgattg aagaatactt gggtgaattt
     6241 acaatgccca ctcatcctct ctatcctctg ttaagacttg ctcttgattt ccgtaaaaca
     6301 ttaagaaaaa aacatagaaa gtaagtatat ggcactaaca acactcacga aagaagagtt
     6361 tcagacttat tctgatcagg tttcttctcg ttcctttatg caatctgtcc agatggggga
     6421 tttgctagaa aaaagagggg ctcgaattgt ttatcttgct ttgaaacaag aaggagaaat
     6481 tcaagttgca gctctggttt atagcttgcc catgctgggt ggtctgcata tggaactcaa
     6541 ttcggggccg atttataccc aacaagatgc tcttccagtt ttttatgcag agttaaaaga
     6601 atatgccaag caaaatggtg tattagagtt gcttgtaaaa ccttatgaaa cttatcaaac
     6661 ttttgatagc caaggtaatc caatagatgc tgagaaaaaa agtattattc aaggtttgac
     6721 tgatttaggt tatcaatttg atggcttaac aacaggttac ccaggtggag aaccagattg
     6781 gttatactat aaagatttaa ctgaattaac tgaaaagagt ttgcttaaaa gttttagcaa
     6841 aaagggtaaa cccttggtga aaaaggctga aacctttggc attcggttga aaaagttaaa
     6901 acgtgaagaa ctatcgattt ttaagaatat aacaaaagaa acctctgaac gtagagaata
     6961 tagtgataaa agtttagaat attatgagca tttttatgat acttttggag aacaagcgga
     7021 gtttctcata gcaagcttga atttttcgga gtatatgagc aaattgcaag gtgaacaaag
     7081 taaactagaa gaaaacttgg acaagttgcg acttgatttg agtaaaaatc ctcattctga
     7141 gaaaaaacaa aatcaactga gagaatattc tagtcaattt gaaacgtttg aagttcgaaa
     7201 agcagaagcg cgagacttga ttgaaaaata tggagaagaa gatattgttt tagctgggag
     7261 tttatttgtt tatatgcctc aggaaacgac ttatctcttt agtggttcct acactgagtt
     7321 taataagttc tatgcccctg cactgcttca aaaatatgtt atgttggaaa gcataaaacg
     7381 tggaatacct aaatacaact tcctaggcat tcaagggatt tttgatggaa gtgatggtgt
     7441 tttgcgtttt aaacagaatt ttaatggcta tattgtacgc aaagcaggta ctttccgtta
     7501 ccatccatcg cctttaaaat acaaagctat ccagttactc aaaaaaatag taggacgtta
     7561 agatgaaaaa gtcagtattt agatttcttt tagcttcttt tagtaaaata attcttattt
     7621 gctagaaagg tggagagaca tgcgctggct ttttcgtttg ataggggctt tcttttcttt
     7681 tgtgtggcgt ttgttttggc gtctggtttg gatagttgtg ctcttatgtg tgcttgcttt
     7741 cggacttctc tggtatctga acggggattt tcaaggagcg ctaaagcaag cagaacggtc
     7801 agtaaaaatg ggtcaacaaa gtattgacca atgggagaaa acagggcaac tgcctaagtt
     7861 aagccagaca gatagtcacc agcattctga aggaaggtgg gcacaggcct ctgctcgtat
     7921 ttacctggat ccgcagatgg attcacgctt tcaagaggct tatttagaag caatccagaa
     7981 ctggaatcaa actggtgctt ttaactttga actcgtgact gagtctagta aggcggatat
     8041 tacggctacg gagatgaacg acggaggcac tcctgtggca ggagaggcgg aaagtcaaac
     8101 taatctctta acagggcaat tcttgtccgt aacggtgcgg ttgaatcatt attatttgtc
     8161 caatccatac tatggctact cctatgaacg ccttgtccat acggcagaac atgagttagg
     8221 tcatgcgatt ggcttggacc atacagatga gaagtctgtc atgcaaccag caggttcctt
     8281 ttatggtatc caggaagagg atgttgcaaa cctccgaaaa atatatgaga ctagtgagta
     8341 gggtactatc tttccctact ttttttgcta taatggaact atgaacaact tgattaaatc
     8401 aaaactagag ctcttgccga ccagccctgg ttgctacatt cataaggata aaaatggcac
     8461 cattatctat gtaggaaagg ctaaaaatct gcgtaatcga gtacggtcct attttcgtgg
     8521 aattcatgat accaagacag aggctctggt gtctgaaatt gtggattttg aatttatcgt
     8581 cactgagtcc aatattgagg cgcttctgct ggaaatcaac ttgatcaagg aaaataagcc
     8641 caagtacaat atcatgctca aggatgacaa gtcctatcct ttcatcaaaa tcaccaatga
     8701 gcgctatcca cggcttatca ttactcgtca ggtcaaaaag gacggtggtc tttattttgg
     8761 cccttatcca gatgtggggg cagccaatga aatcaagcga cttctagata ggattttccc
     8821 ttttcgaaaa tgtaccaacc cgccctctaa ggtctgtttt tattaccata tcggccagtg
     8881 tatggcccac accatctgta agaaggatga ggcttatttc aagtctatgg ctcaggaggt
     8941 gtctgatttt ctgaaaggtc aggatgacaa aatcatcgat gatctcaaga gtaaaatggc
     9001 agtagcagca caaagtatgg agtttgaacg tgcggcggaa taccgtgacc tgattcaggc
     9061 tattggaacg cttcgaacca agcaacgggt catggctaag gatctccaaa atcgcgatgt
     9121 ctttggctac tatgtggata agggctggat gtgtgtgcag gttttctttg ttcgtcaggg
     9181 caagctcatc gagcgcgatg tcaatctctt cccctacttc aatgatccag atgaggattt
     9241 cttgacctat gtgggacaat tctatcaaga aaaatctcac ctagttccca atgaggtact
     9301 gattccgcag gatattgacg aagaagctgt caaggctttg gtggatacca agattctcaa
     9361 accccaacgt ggagagaaaa aacagctggt caatctagcc ataaaaaatg cacgtgttag
     9421 tctggagcag aaatttaacc tgctagaaaa atcagtcgaa aagacccaag gagctattga
     9481 aaatctagga cgtttgctcc aaattccgac tccagttcgc atcgagtcct tcgataactc
     9541 caacatcatg gggaccagtc ctgtttcagc catggtggtc tttgttaacg gcaggccaag
     9601 taaaaaagat taccgtaagt acaagataaa aaccgttgtt gggccggacg actatgctag
     9661 tatgagagaa gtcattcgaa gacgctatgg tcgagtacag cgtgaggctt tgactcctcc
     9721 agatttgatt gtgattgatg gggggcaagg tcaagtcaat attgctaagc aagtcatcca
     9781 agaagaactg ggcttggata ttccaattgc aggtctgcaa aagaatgata agcaccaaac
     9841 ccatgaattg ctctttggag atccgctgga agtggtggac ttgtctcgca attctcagga
     9901 atttttcctc ctccaacgca ttcaagatga ggtccaccgc tttgccatta ccttccaccg
     9961 ccaactgcgc tccaaaaatt ctttctcatc tcaattggat gggattgatg gtctgggacc
    10021 taaacgcaag caaaatctca tgaggcactt caagtctctg accaaaatca aggaagccag
    10081 tgtggatgag attgtcgaag ttggagtacc aagagcagta gcagaggctg tgcaaagaaa
    10141 gttgaacccg caggaaacag aaatcttgct tcaagtggcg gaagaaagag tagattacca
    10201 aacggaagga aaccacaata aaccataaaa tcgcaatttt atcagatatt catggcaatg
    10261 cgacggcgct agaagcagtg attgcagatg ctaaaactca aggagccagt gaatattggc
    10321 tcatgggaga cattttcctc cctggttcag gtgcaaatga cttggtcgct ctgttaaagg
    10381 aacttcctat cacggcaagt gttcgaggca attggaatga ttgtgtcctt gaggctttag
    10441 atgggcaata tggcttagaa gacccacagg aaatccagct catgcgaatg actcagtttt
    10501 tgatggagcg aatggatcct gcaacgactg tctggctacg aagcttgcct ttgctggaaa
    10561 agaaagaaat tgctggactg cgcttttcta tctctcataa tttgccaaat aagaactatg
    10621 gtggtgactt gctagttgag aatgatacag agaaatttga ccaactgcta gatgcggaaa
    10681 cggacgtggc agtttatggt catgttcaca agcagttgct tcgttatgga agtcaagggc
    10741 aacaaatcat caatccaggg tcgattggca tgccctattt taattgggag gcgttaaaaa
    10801 atcaccgtgc ccagtatgcc gtgatagaag ttgaagatgg ggaattggtc aatatccaat
    10861 ttcgtaaagt tgcttatgat tacgaggctg agttagaatt ggccaagtcc aaggggcttc
    10921 cctttattga aatgtatgaa gaactgcgtc gtgacgataa ctatcagggg cacaatctgg
    10981 aacttttagc cagcttaata gaaaagcatg ggtatgtaga ggatgtgaag aatttttttg
    11041 attttttgta agagtttcct aaaatagcca atgcaaacta aaaaagcgat tggctggtcc
    11101 aatcgctttt agt
//
LOCUS       DAVBHS010000062        10920 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVBHS010000062 DAVBHS010000000
VERSION     DAVBHS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10920
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.062"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(6..890)
                     /locus_tag="VQ651_001152"
     CDS             complement(6..890)
                     /locus_tag="VQ651_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HET2754159.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDMIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(894..1280)
                     /locus_tag="VQ651_001153"
     CDS             complement(894..1280)
                     /locus_tag="VQ651_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HET2754160.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLISLMLEEQILTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(1273..2616)
                     /locus_tag="VQ651_001154"
     CDS             complement(1273..2616)
                     /locus_tag="VQ651_001154"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET2754161.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYEVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEGLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(2698..3582)
                     /locus_tag="VQ651_001155"
     CDS             complement(2698..3582)
                     /locus_tag="VQ651_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2754162.1"
                     /translation="MIQVRNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHIEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(3594..4211)
                     /gene="cysE"
                     /locus_tag="VQ651_001156"
     CDS             complement(3594..4211)
                     /gene="cysE"
                     /locus_tag="VQ651_001156"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HET2754163.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFIW
                     KHGFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(4227..6440)
                     /gene="pnp"
                     /locus_tag="VQ651_001157"
     CDS             complement(4227..6440)
                     /gene="pnp"
                     /locus_tag="VQ651_001157"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET2754164.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDDQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRLELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            complement(7125..7991)
                     /gene="metF"
                     /locus_tag="VQ651_001158"
     CDS             complement(7125..7991)
                     /gene="metF"
                     /locus_tag="VQ651_001158"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET2754165.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(8055..10304)
                     /gene="metE"
                     /locus_tag="VQ651_001159"
     CDS             complement(8055..10304)
                     /gene="metE"
                     /locus_tag="VQ651_001159"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET2754166.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVASDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(10565..10831)
                     /locus_tag="VQ651_001160"
     CDS             complement(10565..10831)
                     /locus_tag="VQ651_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2754167.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGVSQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
BASE COUNT         3052 a         2499 c         2166 g         3203 t
ORIGIN      
        1 cttctttaca aaccaactat agttgacaaa gaacctaaaa tcaattgata acacaaggtc
       61 aggtcggtca actctttcaa ctgaagccct gtcaactctt cccatttatc aatcttgtat
      121 tggagagaat tgcggtgcag atagagttgc tgggctgttt tagtgagaac agcactattt
      181 tcccaaagag agagaatgat ttcctgaatc tgatcttgat ccaaaatcat ctggtgtaga
      241 cattctttaa ttggcttcaa gtccacgagt ctttctccca tactccaaag atagagctga
      301 gaaaaagtat gaacaccttg gtgaccctga cgccaccatg tcttgaacaa atcccgctca
      361 gctttgatta agtctgatag ggcttgatgt cctgtctgag accaaacctg acccaacatg
      421 atagaaagac gaagtccaaa gtcatactca actgcttcaa tcatatcact taaaatatct
      481 cttacagaag tgtatttgtc ttgttgaagc acgaaaacat aatcctgagc tccgacctgt
      541 aacactgtct gacaattcgg aaaaagagtc cgcatcatat ctagccaaga agccagattt
      601 tcctgctgaa aataagaaag atggcaataa accaactgaa tctttttaaa aacttgcggt
      661 gcctgtccct tgccttcaac cagataggaa taccaagggt ttagcgaacg agcctgctcc
      721 tgctgggtca aaagggcaac caactgcttt tcacgctcgc tgagcccagc ttcctccagc
      781 aaaatccact gctgagaagc taaaggaagc gtgagatagc cctctttctc tactggttgg
      841 tctgaaatcc gagcctcagg aaaccagtct tgtagttctt ttgccctcat gttctagccc
      901 tccacttttt ggatgcacca tgaaaccaaa ctctcaagac gttccagatt ctcagtcata
      961 tggagatagc ccataactgc ttcaaatccc gtggacatac gataagtcac gacatctgca
     1021 tttttagcct ttgtgtgact attggtattg cggccacgtt tgtagatttc ttcttctttt
     1081 tccgttagga tctgctcctc caacatgaga gaaatcaggc gagcttgagc cttggctgac
     1141 acgtacttgg tcgcctcttg gtggagttta ttgggcttgg tcataccttt gaggatgagg
     1201 tggcggcgaa tatacataga atacaccgca tctccctcaa aggctagcgc aatcccgtta
     1261 atgagattga catcaatcac gtgtccacct cactccatcc ttggtatcaa ggagcttaat
     1321 tccttgagta accaattggt cacggatttg gtctgctgtc gcaaagtcac gattggcacg
     1381 cgcctcttgg cgtttttgaa tcaagtcttc aatctctgca tccaaaactt cctcaacaaa
     1441 gacaattcca aaaatttcta acatatctgc aagagcttgc ttgacacttg catcatagtt
     1501 ccctgagttg atccatttgg ccatttcaaa gacaactgtg ataccgttgg cagcattaaa
     1561 atcttcatcc atagctgcta caaacttatc tttaaagttt tgtaactctt gggcatccac
     1621 gtttcctgta aatggttgtt cgtaagtatt cttcagatac ttgagattgg tctcggcatc
     1681 gcgaactgcc ttttccgtga agttgatagg cttacggtag tgctgggtcg caaagaagaa
     1741 acgaagtact tgcccatcaa gagttttaag ggcatcgtgt accgtaatga agttacccaa
     1801 ggacttagac attttgacat tgtcgatatt gacaaagcca ttgtgcatcc agtagttagc
     1861 aaaagccttg cctgttttag cttcagattg ggcaatttca ttggtgtggt gtggaaactc
     1921 taggtcagct ccaccaccgt gaatatcaat ggtatcacct aaaatctctg tcgacatgac
     1981 tgaacactca atatgccaac ccggacgtcc aggtccccaa ggactatccc aagaaatctc
     2041 acctggtttg gaagatttcc atagagcaaa gtctacagga ttttccttac gagccgtttc
     2101 ttcatcggta cgacctgaag cacctagctc caaatcttcc aaggttttat tagccaattt
     2161 agcatagttg tgggattttt ctacacggaa atagacatcc ccttgactct cataggcaaa
     2221 gcctttctcg atcaagcctt ccacaaaacg gatgatgtct gccataaact ccactacacg
     2281 cggatggcga gtcgcaggtt tcacgcccaa ggccgtcaca tcctcacgaa aggcagcgat
     2341 gtacttatcc gcaacctcct gaggcgtgat gccttcttcc ctggcacggt tgataatctt
     2401 atcatccaca tccgtaaaat tggaaatata ggcaacctcg tacccacgat actcaaaata
     2461 gcgacgaatg gtatcaaaag ctaccgttga acgagcattc cctacatgaa tatagttgta
     2521 caccgttggc ccacaaacat acatcttgat cttgccgtcc tcaatcggga caaattctcg
     2581 caaatcacga gacatggtgt catagatttt aatcataaat cataatcaag aaagctgaaa
     2641 tccaagaaca attagtttca tcactaaaag ttcaagtaaa tttcagtccg aatatctcta
     2701 cacttcggaa tcccttgctc ctttctcatt cagataaacc acctgagtct gtttgacaaa
     2761 gccaattttt tcatacaaac gtttggcacc tacattgcta tcttccactg caatctgaaa
     2821 ttccttgtca ttttgctcaa ttagttggtt gacgagggat tttgctaagt agcttccata
     2881 gcctttccca cgttcaggtt ccgatattgc taaaccgtag aagtaattcg tattagtcga
     2941 taaatctacc gtgcaagtcc caataacctg accgtctttt aacaaaatat atagtcggct
     3001 ttctggatcc ttcagagctt cagcgacata tctatccaca acttctctcg attcatgttc
     3061 ctctgaaaat gcctgaaatt ttaattgact aatttgatcc tgatacgaac tatctgctaa
     3121 caaaacttca atatgggaaa catttgctaa cggataaggt cttctatcct tacctaacca
     3181 agtttctgtc tcttcatcct cgattagtcc ccagttactg gcaaagtcag gatgattctc
     3241 taaaaaaata cgttctgtct gaaaagtgac tgatcgaatg gggaaagaag ctgtttctct
     3301 ctcaaaacta gtaaacaatg cacgcgcaat cccctgacgg cgatgacctg gatgaaccag
     3361 tatcgtcact tctacatctt ggtcatctgc atagacagtt aataaaccaa caagttcgcc
     3421 tttttcataa taaaggaaaa aggcgggcat gtttgggtca aaattaagca tgttagagag
     3481 atagggatcg cgataggtac cgtcatagtt ttggcaacag ttaattactt ttttcgcctc
     3541 agatagctcc tcttggctta acttgtttct tacttgaatc atataggtat cctctacaaa
     3601 ccagacgatc tgtgactggc atctttagcc tgctcgagtt tattgacata atactctcgt
     3661 ttttcttcga cttcgtgaat gacaggctca tctttcttac catgaagacg gacaatcttg
     3721 gccggaatac cgacaaccgt cacatcacta ggtacatctg ctacgacaac tgctgcagca
     3781 ccgaccttgg cattttcacc aatttccaca ggtccgataa cttgggcatg ggctgatatg
     3841 agggctccct ttcgtacagt cggatggcgt ttgccacagt ctttccctgt tcccccaaga
     3901 gtcactccgt gatagagaag aacgcctttt tcaacaatcg ctgtctctcc aatcaccaga
     3961 ccagaaccat ggtcaataaa gacacctgaa tcaatctggg ctcctggatg aatctcaatc
     4021 tgagtccaaa agcgccaaaa ctgactgtgc atacgagcta agagtttgaa gccgtgcttc
     4081 cagataaaat gcgagagacg gtgggccgcc aaggccttga cacctggata agtcagcaaa
     4141 acctccaaag tggtgcgggc cgctggatca ttttctttta cgatatcaat ggtttcgcgc
     4201 caccacccca tacatttctc cttttcttat tctgaatctt ttggtgtttc tgtaaattct
     4261 ttcttaggtt tgtgatcctt gtgatgacgt gggcggtgag gacgctcaga cttttcacct
     4321 ttttcatcat gctcaggttt tggcggacga ggtagaagag ccttcataga ggcatcgata
     4381 cggccttttt catcaatctt gataacctta acatcaactt catccccgat ttctaccaaa
     4441 tcctctacac gattggtacg agtccaagcc atctcagaga tatgaacaag ggcatctgtc
     4501 ttatcaaaga ggttaacaaa ggcaccaaat ttctcgatac gaacgacttt agcacggtaa
     4561 acttcatcca ctttggcttc acgaaccaaa ccagcaataa tttctttggc acggttaata
     4621 gcatcttggt cactagagta gatagacaca tttccttctt cgtctatatc aatcttaaca
     4681 cctgtttcag cgataatctt gtcgatggtt tctccaccct taccgatgac aatcttaatc
     4741 ttgtccacat caatcttgat cgtatcaatt ttcggagcag ttggagccaa ttctagacga
     4801 acttctggaa tggttgcttc aatgacatca aggatttcaa aacgcgcttt cttggcttga
     4861 gcaagagcct ccgtcaagat ttctgcagta atcccttgaa tcttgatatc catttgaagg
     4921 gctgtaatcc catcacgagt acctgcaacc ttgaagtcca tatctccaaa gtgatcttcc
     4981 aaaccttgga tatctgtcaa tactgtgtag ttatttccat ctgagataag tcccatagca
     5041 ataccagcta ctggcgcctt gattggcaca ccaccagcca taagggcaag agttcccgca
     5101 cagatagaag cttgagatga agaaccgttt gattccaaaa cttctgctac tagacggata
     5161 gcgtatggga attcttccaa gcttggcaag acttgagcaa gagcacgctc accaagggca
     5221 ccgtgaccga tttcacgacg acctggcgca ccgtaacgac ctgtttcccc tacagaatat
     5281 tgagggaagt tatagtggtg cataaagcgt ttcttgtact ctggatccaa accatcaatg
     5341 atttgagttt ctcccatcgg agccaaggtc aagactgaaa gagcttgagt ttgcccacga
     5401 gtaaagagac ctgaaccatg tacacgagga aggaagtcaa caaccgcatc caaaggacgg
     5461 atttcatcga ccttacgacc atcaggacgc accttgtctt ctgtaattaa acgtcgcact
     5521 tctgcgtgtt ccatttgttc caagatttca gccacatcac gcataatacg gtcaaattct
     5581 tcgtggtccg catatttttc ttcgtaaacg gcagtcactt ggtctttcac tgcttgagtt
     5641 gcagcttcac gggccaattt ctcttctact tgaactgcct tttggaggtc actgttgtag
     5701 gctgcaatga tttcagcttg caattcagca tccacgtgaa gcaattccac ttctgctttt
     5761 tctttaccga cagcagcaac gatttcttct tggaaggcaa tcaattcttt gacagcttcg
     5821 tgccctttaa gaagcgcttc caacatgatt tcttctgaca attctttggc accagactct
     5881 accatgttga tagcgtgctt agttccagct actgtcaatt caagaagaga ttgctctgct
     5941 tgttcttgac ttgggttgat gatgatttgg tcatctacat atcccacttg taccccagca
     6001 attggtccgt caaatggaat atctgaaata gacagtgcca aagatgaacc aaacatagca
     6061 gccattggtg cagatgcatt ttcatcataa gaaagcactg tattgatgac ttggacttca
     6121 ttacggaaac cttccgcaaa cataggacga atcggacggt caatcaaacg cgctgtcaag
     6181 gtcgcatctg ttgaaggacg tccttcacgt ttcataaagc caccaggaaa cttcccagcc
     6241 gcatacattt tttcttcgta gttgacttgg agtgggaaaa aatccccagt tgccattttc
     6301 ttagacataa cggcagcagt caagacagtt gactcaccgt aacgtacgac aacagagcca
     6361 tttgcttgct tagcaacctg accagtctct acaattaact cacgacccgc aaaagtcgtt
     6421 tgaaacactt gttttgccat tttaatcccc tttggattga tgaaattata cgccttgcct
     6481 acaaagatca agataccaag gacgtcaaaa gcaaagtaaa aataggaaac tgacgaagtc
     6541 ttcgatgaag acaagacagt ttatcttttt tacacagctt ttcggccgtg ttcaattaca
     6601 caagatattt tggacggttc ggcttgccga acatttctgt agaaaaatag gaaggtgacg
     6661 tcgcactcga cgagtgctag gaagcttatc ttttttccta agaaatgaga ccaaaattca
     6721 agtcatcaag ataccaagcc gtcaagcaac tcaaaggaag ataggaaatc gaacgacgga
     6781 gcgactgctc ctaggcagat ttatcttttt ccacagagtt gtaggcgtgt tcagttttca
     6841 agatacatca ttagaaaggt ttaatattaa agtatctaaa cctttcacgc taatcgctat
     6901 cgggcgatta gctaaatgct ttactaactc tttcgtcaaa taacatcgat ttgactcact
     6961 cgtgtcgtta aatcttacag tttaaatgca ttgtattatt taataccttc atctttgtat
     7021 caagtacgta cagaatctat tttatcatat ttttcttaaa aagtgaggtc tttaccatta
     7081 aaaaggaacc attcccctca cctgagaaga atggtttgct tttattatcc tagagactgg
     7141 tgattaaaca aggcatgggt tgcttgatgg atgtattttg ctgtatcagc attattcatc
     7201 gtatagagat gcacaccggc aacatcctga gttaccaagt ccacgatttg gtccactgca
     7261 taggcaagtc ctgctgctct gagcgactca gggtcatgct catacttgtc taagatggct
     7321 ttaaatttgc gtggaagatg gatattctca caagtcttca agagtcggag agcctgattt
     7381 cgattcagaa ttggcataat tcctgcatga ataggaacat caatcccagc caagatacac
     7441 ttgtcctgaa aatcatagaa gcgctcattg tcaaagaaga gctgagttac gaggctcgaa
     7501 cagcctgcat ccactttctt cttaagattt tgaatatctg aaatctgatt tggcgaatct
     7561 ggatgccctt ctggatagca agctccaata atatcaaagt gaggggcttg ttccttgata
     7621 aactcaatca agtcggttgc atagcggaaa tccttttgtg gttctacgtc tggaataata
     7681 tccccacgaa gagccaagat tttctgaacc ccaactttgt ccaagtcagc aatagtttca
     7741 gcaaccttgt ccttagttag ataaatagct ggcaagtggg caatggtcgg aatcgccaag
     7801 tcattttgga taaagtcagc caaacgaacc gtcgtttcct tgatattaaa tttattattg
     7861 ctggcagtta cactgataaa atggggagcc aactcctgca tatcctgcaa ggctgaaata
     7921 atgttaccat tacccacggc tgggtttgga gggaacactt caaatgagag tgacggtgtt
     7981 tggcgtgaca tatgtaataa ccttttctag ttgatttctt tttgaacaac cactgtatgg
     8041 agagaaatcc aatcttacaa tttctcacgc gcagctttag ctgcttcaac aaggcggatc
     8101 aagctttctt ttgtttctgg gataccacgt gttttcaaac cacagtcagg gttgatccaa
     8161 actttcttgc ttggcacttt agcaaggatg gcttcgattg tgttgtcgat ttcgccttca
     8221 tttggtacac gaggtgagtg gatatcgtaa accccaggtc ccacttctgt ttggaagttt
     8281 ttcgctttga gttcgtccaa gatttcaagg tttgaacggc tggcttcaaa ggaaataacg
     8341 tctgcatcca tgttatcgat agctgggatg atatctgtaa attctgagta acacatgtga
     8401 gtgtggattt gtgtgtctga cgctactgtt gagtgtacca agcggaaggc aggaattgcc
     8461 cagtcaaggt aatcttcgta ccagtcacta cgacggagtg gcaatttttc acgaagagca
     8521 gcctcatcaa tttggatgat tttcacacca gcagcttcaa ggtcaagcac ttcatccttg
     8581 atagcaaggg cgatttgaag agtagaatcc ttgatagaga tgtcttcacg tgggaatgac
     8641 cagttaagga tggtaacagg tccagtcaac atacctttaa caggtttgtt tgtacgactt
     8701 tgtgcatagc tagaccattt aacagtgata gggttaagac gagtgacatc accccagatg
     8761 attggtggtt ttaccccacg cataccgtat gattgtaccc atccattttt agagaagagg
     8821 tatcctgaca agttttgacc gaagtactca accatgtcat tacgctcaaa ttcaccgtga
     8881 acaaggacat caaagtcaat atcttcttgc cacttgatcc attcgtcaat cgtttcagca
     8941 aggaaagcat cgtactcttt ttgagacaat tcacctttac ggtaagccaa acgtttggca
     9001 cgaacttctt ttgtttgagg gaatgaacca atcgttgttg ttggaagagc tggaagtttg
     9061 aaagcttctt cttggatagc ttcacgttct gcaaaggctg gcaaacgagt gtagtctgcg
     9121 tctgtcaagc cagcgatacg cgcacgaagt tcagcatttt caccaacacg ctcagtcgca
     9181 aagagttctt tgttggctgc aagagcttct gaaccttgac cattgcggat agcatccaag
     9241 tcacggattt catccaattt ttcaactgca aaggcaaagt ggttcaagag tgctggttca
     9301 aattcttcat tagcagttgt aaatggcaca tgaagaagtg agcaagagct tgtcaaaacg
     9361 atgttttcag ctggaatttg ctcaagaaca gccaagctct tttcgtagtt gttgcgccag
     9421 atgtttttac cattgacaat acctacatag agagtcttgt cagctgggaa gccaccttta
     9481 acgagttcaa gagttttctt accttcaaca aagtcaagac cgatagcatc tactggtaag
     9541 tttacaaggt cagcgtatac gtcacgaaca tcaccgaaat aagtttgaag caagacttca
     9601 agaccttttt tgtcagccaa gagtttgttg taaaggttca agaagagagc tttttcttca
     9661 gctgtcaagt cttttacaag agccgcttca tccaattgga tgcgagtcgc accaagttca
     9721 gccaatttag caaaaacttc ttggtaagca gccactaagc tatctacgaa gtcgtctgct
     9781 ttcacgcctt cttcaaagtc tgacaattga aggaaagtga agggacctac aagaacagga
     9841 cgagtgttca atccaagttc tttggcttct tggaactcat cgaaaatctt gtgaccagcc
     9901 aattttactt gagtgtcttt ttcaaattta ggaacgatgt agtggtagtt agtgttgaac
     9961 catttcttca ttggaagggc gcgaacgtcc cctttttctc cctggtaacc acgtcccaaa
    10021 gcgaagtagc gctcaaggtc agacaagtcc aagttttgaa cggatgcagg caccacgttg
    10081 aaaaggaaag ccgcatctag gaagttatca tagtgagaaa agtcatttga tggaatttca
    10141 gtgatgcctt tttctttgac aatgttccag tgtttagcac gcaagtcttt tgctgctgct
    10201 aaaagttctt cttctgagat ttcttttcta aagtattttt cagttgtaaa ttttaattca
    10261 cggaattcgc ccaaacgagg gaaaccgatg attgtagttg acatgatgtg tcctccaaaa
    10321 tttgttgttg aaactatctt aacagaaaag aaagcgtctg tataattgta aaaaattagg
    10381 gtttgatata gtttgaaact atatatctgt ttcggacaaa agaaaaagac ttgaagcaaa
    10441 cgtctcaaat cctttgtaat tcttacttta cagctatatt ccaattagaa tactaaaaca
    10501 tgttattagt aattcttata agtgactatg acctgttatt agaaaagact ataactgatt
    10561 ctagtcaact tttcccctgt tcaagtggaa cgactgctag tgtctttcct agactggtta
    10621 gaacttttaa gaccgtatcc aactgaagac tgctctctcc tgtttccatc ctagctatga
    10681 caggttgact cactccactg acttcttcca gctttttctg actgattcct tgttcatacc
    10741 tagcctcaat caactcgctc atgatagcca ctcgcatatc actttcaagg atttcctcct
    10801 tgctaaagag ctcagatgga catccttcca attactccca atagcactat tcttcatcac
    10861 ttaaccctct ttcttttacg tctatgtatt tttaaaagaa tgagcgaatt atgattcgat
//
LOCUS       DAVBHS010000063        10740 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVBHS010000063 DAVBHS010000000
VERSION     DAVBHS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10740
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.063"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..156)
                     /locus_tag="VQ651_001161"
     CDS             complement(<1..156)
                     /locus_tag="VQ651_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET2754168.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRK"
     gene            complement(137..322)
                     /locus_tag="VQ651_001162"
     CDS             complement(137..322)
                     /locus_tag="VQ651_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754169.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(626..2188)
                     /gene="guaA"
                     /locus_tag="VQ651_001163"
     CDS             complement(626..2188)
                     /gene="guaA"
                     /locus_tag="VQ651_001163"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET2754170.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            2330..3028
                     /locus_tag="VQ651_001164"
     CDS             2330..3028
                     /locus_tag="VQ651_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2754171.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(3071..3967)
                     /locus_tag="VQ651_001165"
     CDS             complement(3071..3967)
                     /locus_tag="VQ651_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET2754172.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLLIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(3976..4911)
                     /locus_tag="VQ651_001166"
     CDS             complement(3976..4911)
                     /locus_tag="VQ651_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HET2754173.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4908..5633)
                     /locus_tag="VQ651_001167"
     CDS             complement(4908..5633)
                     /locus_tag="VQ651_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HET2754174.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(5717..6352)
                     /locus_tag="VQ651_001168"
     CDS             complement(5717..6352)
                     /locus_tag="VQ651_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HET2754175.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(6354..7181)
                     /locus_tag="VQ651_001169"
     CDS             complement(6354..7181)
                     /locus_tag="VQ651_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2754176.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(7203..7539)
                     /locus_tag="VQ651_001170"
                     /pseudo
     CDS             complement(7203..7539)
                     /locus_tag="VQ651_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
     gene            complement(7540..8256)
                     /locus_tag="VQ651_001171"
     CDS             complement(7540..8256)
                     /locus_tag="VQ651_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET2754177.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISMADII
                     EKAGITPIGVYTHEPRDQAPLVIKEVNAIKVALLAYSYGFNGIEQYIFQEDYNRYLSD
                     LNEDKIKAEVERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSKTVEKDGDKKLIIY"
     gene            8779..9390
                     /locus_tag="VQ651_001172"
     CDS             8779..9390
                     /locus_tag="VQ651_001172"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET2754178.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEGIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9435..10163
                     /gene="rlmB"
                     /locus_tag="VQ651_001173"
     CDS             9435..10163
                     /gene="rlmB"
                     /locus_tag="VQ651_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET2754179.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFLNRL"
     gene            complement(10202..>10740)
                     /locus_tag="VQ651_001174"
     CDS             complement(10202..>10740)
                     /locus_tag="VQ651_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2754180.1"
                     /translation="GVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLTRFAKTVTIV
                     HRRDQLRAQKVLQDRAFANEKISFIWDSVVKEIKGENRVESVVFENVKTGQVTEQAFG
                     GVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLKDLRQVTTAV
                     GDGAIAGQEAYKFITEHS"
BASE COUNT         3274 a         2401 c         1935 g         3130 t
ORIGIN      
        1 tttacgtcca ccacaaaaga gaaaaacttg acctgagaaa ggatctaatt caaagtgggt
       61 tttaaccaga taagccagtg aatcaatgcc ttgcctcata tccgttttcc cacatacgag
      121 atagacctgc cctaggctag atagatgaat tgtcatagag caacacctta tccaaaagct
      181 gttctaccat ttcgagattg agagattgaa aaaaacttac ttcaagcttg ccaatacgaa
      241 ttttaagcag aatatcattt ctctttttag aatcaaaagg acgagattgt ggaatctcta
      301 caggaacaat aggttgtggc attaaaatat cctccaatag ttttacttta ctaatagtat
      361 acaggaggaa ctccatgaat gatagatacc ttgttatgac gcgattacta tattagaaag
      421 ttggctctct aattaaatta attctcatta tatatatcaa aagaccgcag cttgaaaact
      481 acggtctaat tttttgtcca aatgtaaacc acttggaact attattcaat ttattctttt
      541 tagtaaatgt tcccgtttta atcccagtta cagtttttaa tcctctgtaa ccgttgattt
      601 tacaaagctt ttaatttttt tgcgattatt cccactcaac tgttgcaggt ggtttacttg
      661 taatatcgta gacgatacgg ttaacatgat ccacttcatt tacgatacgt actgagattt
      721 tttgaagtac ttcccatgga attttggcaa aatcagcagt cataccatcg atagaagtga
      781 tagcacggat tgcaatcgtg tagtcatatg tacgaccgtc acccataaca ccgactgaac
      841 gaacgcctgt gttaacagtg aagtattgcc aaatatcgcg gtcaagtcca gctttagcga
      901 tttcttcacg aagaatagcg tctgattcac gaacggtttc aagtttctct tcagtgattt
      961 cacccatgac acggatagca agtcctggtc ctgggaatgg ttggcgccat acgatatggt
     1021 ctggcatacc aagctctgta ccaagagcac gaacttcatc cttgtaaaga gtattgagtg
     1081 gttcaatcaa ttcaaactgc atatcttctg gaagaccacc cacgttgtgg tgtgacttga
     1141 tagtttgagc tgtatccgta ccagactcga taacatctgt atataaagta ccttgagcaa
     1201 ggaatttcac atctttgagc ttgcttgctt cgtcatcgaa tacatagaca aactcgttac
     1261 cgatgatttt acgtttttgt tcaggatcag aaacgccagc aagtttgtca aggaaacgtt
     1321 tagcagcgtc tgctttgacg atattcaaac caaacttacc accgagcatg tccataactt
     1381 gatcagcttc gcctttacga agaagaccgt ggtctacgaa gatacagatc aattgatcgc
     1441 caatcgcttt ttggagaaga accccaacga cagatgagtc aacaccacct gatagaccaa
     1501 gaaggacacg tttatcaccg acggtttcac gaattttttt gatctgcatg tcaatgaaat
     1561 tatccattga ccagtcacct ttagccttac aaatgttaag ggcaaagtta cgaaggatat
     1621 catttccgta tacagaatga cgaacttctg ggtggaattg gataccgtaa atgtgtttat
     1681 ctgggttttc gatggctgcg tatgggcagt cagctgatgt acctgtacga acaaagtcag
     1741 caggaatctc agtaaccgca tcaccatggc tcatcaaaac agtctgttca tcaggtgttg
     1801 attcaaaaag cgctgatggt gtgtgagtta gggttgattg accgtattca cgatttccag
     1861 catcacctgc aggaacaact tttcctccaa gtttatgggt caataactgc ataccataac
     1921 agattcccaa aattggaatt ccgagttcga agatttctgg gtcaatatca aatgaaccat
     1981 cttcatatac agaatttgga ccacctgata gaataattcc tacaggattg acttcacgaa
     2041 cttcagcagc tgaaatttta tggcttttta gttctgaaaa aacaccaatc tcacggatac
     2101 ggcgtgaaat cagctggttg tactggctac catagtccaa tacgatgatt ttttctacat
     2161 cttgcaaatc agttgaaatg ttgctcatct ttttcctttc tcaaaagaaa tatcttttct
     2221 caatattcta tcacaaataa gggcgaatta ccaactaaac aaggcgaagt ttgacttttt
     2281 ctagtttatt ggggtacaat agagaaaaga tagaaatgaa gtgaaaaata tgctacctgc
     2341 ttatatgaaa atccatgatc agataaaaaa ggatattgac gagcaccgtt gggctattgg
     2401 tgagaggctt cccagtgaaa gagatttagc tgagcagttt gcggtcagtc gcatgaccct
     2461 ccgccaagcc gtatctctat tagtcgaaga aggcgtctta gagcgccgtg taggaagcgg
     2521 cacctttgtt tccagtactc gagtacaaga aaagatgcga gggacaacca gttttactga
     2581 aattgtcaaa tcccaaggta aagttccctc tagccagctc atttcctaca aaaaaaccat
     2641 tcccaatgag caagaagttg ccaagctagg aatttttcca acggacaata ttatccgaat
     2701 ggagcgggtc cgctatgccg accaagttcc cctagtttat gaagttgctt ctattcctga
     2761 aaaattcatt aaggacttta aaaaagaaga aatcacaagt catttcttcc aaaccttgca
     2821 aaaacatggc tatcgtatcg gcaaatctca acagaccatc tatgctcgcc ttgctaaaga
     2881 aaagattgcc cactatttgg aagttgaaaa aggacatgct attcttggat tgacccaggt
     2941 ttcctaccta gaagatggta ctgcttttga atacgtaaaa agtcaatatg taggcgaacg
     3001 ctttgaattt tatcttgaaa ataattagat taccacgcaa aaactctctg tcacggacaa
     3061 agagtctttt ttatttttct aagaataaat ctttcaaaga aatcaaagtt acagcaaaca
     3121 atatcattgc cataccaaga aaatcaatgg gataaaattg ttcattcatt attaagaagg
     3181 caaagaaaat cgccgatatt ggctcaattg aagccaacaa gcttgacttg accggtccta
     3241 tcagactggc tcctttaagg aaagctgtat aggcaaagac agtcccgata aggataatgc
     3301 ctgcaaacgc aaggagaaaa tcaagactag tcgggatatc ggcctgtaga acccctgtaa
     3361 aaggaagggc gaccaaacct gctatgacca ttcccacacc aatgagcaag ctgctccccc
     3421 actttttaat caaggctatg ggtaaaatga tatacagagc ataagtcaag gcagaaaaga
     3481 gaccccagaa cagaccagca ggtgtcatgg ataactggtc caactgccca tgtgttgcga
     3541 tcaggaaggt tcctccgatg gcgaatatga tggaaactat ctctcccagt gtcggtgcca
     3601 ccctatcctt gatacagcta taaattaaaa ttccgacagg acaaacatac tgaagcaccg
     3661 tcgctgttcc cgcattggtc tcctgaatag cagacagata ggcgaattgg ttgagaaaaa
     3721 gaccaatcag agcaaaaata agaagagaca gcaaactctt tctatccttt aaaaagacca
     3781 gtattttatc ctttgcagta gcataagcca agagcatgag aattccacca gcgattaaaa
     3841 gacgcaagtt ggtcaagacc agagccgaaa ttccgtgtgc cattaggtat tggccactcg
     3901 ttcctgacaa cccccaagca ataccagcca caactgttag taaagtccct tttaaactat
     3961 ttgacatgtc tctccttact cttctttgcg aatcaagttc atcacttgat tgcggtctaa
     4021 tatccattga gctctctcgt ccttttcata ggtacgattc gataggaaaa tagtggcttc
     4081 ttgcttctga cgattccaca tgataaaggt acctgtatag cccgtatggt ctagccaatc
     4141 tccttccaaa ttccatgcta aagaacgttc cttgtcatcc aaaggagaaa aattttgatt
     4201 taagtctctt gcaaaatcat ctgctaaata gtgttctaaa aagatttgta aatcctttat
     4261 agtcgaaaat aaaccagcac tcccagcatg tctacccagg agacgagcct tgggatcatg
     4321 cactatgcct gcctctacac ctctaactgt tggaacagca agctcaactg gcccaaactt
     4381 ggtttccgtc attccccaag gtttccagac ttgatccttt aaaatcacat ccaaatcttg
     4441 attaaaaatt ctttccaaaa taaagcccaa cagcaaaaaa tggacatccg aataaagaaa
     4501 ggctggctga cttcgtctgt tgagatgaaa catcgcttcc tttaattcag gggctgttaa
     4561 aagatcacga ttaggaataa aaggatcaag gtctgttgca tgagtcaaga gctggcgaat
     4621 agtgatgtct ggataatcac tctcaggtaa aaaatctatt accagtctat caatatctaa
     4681 ttgacctatt tcccacaaga aggtacaaac tgtgccaacc ccaacaacct tgctgacact
     4741 agctagatca taaactagtc ctgcctcagt ctgcaagcca tgctctgggt cactctggcc
     4801 taaatagaac tctgtccatt gattgtcctt aaaatacgca aaagaggctc cgggataaat
     4861 tcctgcctcg atttgttctt ctattttttt aataatcttg gtccacttca tacttcttca
     4921 aaccacaatt caacattatt tctatcttta ccaaggaaga atttttcaga cttaggaata
     4981 aaatattcag tagactcaaa cttctggcga agacttgcta tgtctaattc attgaccaag
     5041 aacttgagca tagataagtc ccaggtaacc gtattgtcca cagtcaaatc ctgcccctga
     5101 gctggaataa aatcaaggga tgccccaatt tcagatgatt ccaagaaact ttcgatatca
     5161 gttgggagat gtaattccat agaaatctca aatttactta aagaaattga tgccaaatct
     5221 gtttgaaatt caggcttttc tcctacttct actagacttg ctctgtcatc ttccgcatga
     5281 atcaaaatca aatcatcttc tggtgagaaa atttcaaaag cgtagccatt ttgaccttta
     5341 tataatcgat gaatcgaatc tgttttagat aagattcctt caatttctaa gggattttcc
     5401 accttgacaa tcaatctagc tagttttttt cttccctcta ccttacgagt acgcatactg
     5461 ggagcttctt ctaaaaccag cttttcaaga cccgtttggt cacctagtga cagaaaggcc
     5521 gattcttcta acaaggcctt cattccaagg gtttcaatat aaaatgtttc atttaatttt
     5581 ctattattag cttttaaagt aggaataatc cgtacaatct gatttacatt cataaattcc
     5641 tccgtcactt tttattttaa aggattttag aattttttac aagatattat gctcaaattt
     5701 aacattattt agctatttag taaagatagt ctttcaagga ttttgacaaa gcctgataac
     5761 cagcaatact gaggtgcagt ccatcagttg tatattcttt tttgagttgg cctgcttggt
     5821 ctgtcaaaca atcaaatact ggcacaaatt ccacctgcat ataggcagat gcaagctctt
     5881 gataggcttg attccagttc tgaatttttt cattcgagcg gatatagact gcctgctggt
     5941 actcctctcc ctcattgaca ggcaaaatgg aaagcaattt aatctctgtc aatggataat
     6001 cgcgagcaac ggattgaatg atagcttcga gattattgag agcctcattc acaggaacat
     6061 cctttccgat atcatttgtc ccaatcagaa gaaaaatttt atctactgct ccaccatata
     6121 gatgagcatc aagattctct agtaacagtc ctgtctgata gccacgaatt cctcgattga
     6181 caatcgtctt tgaagtccca aatagctcct gtagaggata atactcgaca atggaatccc
     6241 caataaaaag aatgtttggt tctacaacag aaatgtgatt taggtgacga tacttagttt
     6301 gaattttttc ttgttccttt aggagccaat tttctaataa ctgtactgcc acttcattct
     6361 cctttttcta accagttttc caagacttga taaactcctc cttggctgtt ggctggtgct
     6421 agagcagtcg ccacagcttt ggctttctca tcagcatttt ccatcgcata ggcaattcca
     6481 gccatttcaa gcatttcaac atcattttca ctatcaccaa aagccatgat ttcttgggat
     6541 ttcaagtccc agcgcttgag taattcctcc aagccccatg ctttatgaat cccagcttgg
     6601 aggatatcaa tgcaaccata gccactggat acagctcgga cacggccatc aaagagagca
     6661 ttgatttctt ccaaaaccga actcaaacgt tcctcaccaa caaccatgct catcttgagc
     6721 acaccaccaa agaggtcaga tgttaattca tccacaaatt gcatccgttg gtagaatttt
     6781 tcaatcattt ctggagtcat aaaactttca agatctgtaa aaatcgtacc ttccttgaca
     6841 aaatcaccct tcatccccgt tacaacaaac tggtcctgac acgctcgacc cttgaaatga
     6901 gccaaagcct tgttgacaat ggcgtcatcc catgtctgag cctgaatcaa ttcattgttt
     6961 tcaaaaatac gagcgccatt agcaacaacc agaaccactc gatccaccaa gggactcagt
     7021 agttgtctca tgcggtgaat ttcattgccc gtcgcaatga caaaacgaat gcccctttga
     7081 tccaactgat ctaaaatctt ttccaatcgt gggagatcaa gctgacccct agcatccagc
     7141 aaggtcccat ccatatctgt cgcaattacc ttaatcatca tttactttcc tctggattca
     7201 agctaatacc acttcaatcc cacatgttca ttcatttctt tataggctgt atcaattcgt
     7261 tccttagtcg cttcatctaa ctggtcacga tgactgccac ccctctataa aatcttccaa
     7321 aatataagtt tggtaatgat acaagggata tccttctggt gaaaaggttc cctttggtgt
     7381 tcgattgacc caagtaggat gagctttagc tgttccgata gttgtttttc catccttctt
     7441 cttgatggtg acatccatga gaacaccacg ttcagtccac ttagcattct cttcatctcc
     7501 catagattca atccgttgat tggaaatgaa gttccccatt taatagataa tgagtttctt
     7561 atctccatct ttttcaaccg ttttagatgg ttcaacaacg tgaggatgcc ctccaaagat
     7621 aatatccgct ccccaatcga tcatcttgtg ataaagagct ttttgttctt cagttggttc
     7681 caatcgatac tcaacaccca tctgaggcat gataatggtg atatctgctt ccttctctgc
     7741 ccgttcgact tcagccttta tcttatcttc gtttaaatct gaaagataac gattatagtc
     7801 ttcctgaaaa atatactgct caattccatt gaaaccatag gaataggcta acaatgcaac
     7861 cttgatagca ttcacttcct taatgaccag cggagcctga tcacgtggtt cgtgcgtata
     7921 aactccgatt ggagtgattc cagctttctc aataatatcg gccattgaaa taactccctc
     7981 aatttgcgaa tccaaaatat gattatgagc taaatccagc acatgataac ctgcttcctt
     8041 aatagcatcc ataacttcaa caggagcatt aaagagaaga taacccgcta aataatgatc
     8101 cttattaatg gttccttcaa aatcaccaat agctaaatct gcttgcttga gccaaggagt
     8161 cacatactca aaattttcat gaaagtcata ggtaccgtct tcttttttag ctgaaaagaa
     8221 aagtccatcg tggtagagca aatccccgtg ggccataatt ctggcagttt tttcctcttc
     8281 ctactcctga gacttttgct tagtcccctc ttgagaaata gtatctcgtt tttgactagt
     8341 caagggattc cttggaagct ttcaaacaac aagaccaagc ccattgataa accaactgct
     8401 agcaagagta tcgccacaaa tcccttattg ctccacttgc gataactcct aaaaagttta
     8461 ccaagccctt cataaaacga aaagctaaac caccctgatt tcgattttgt cttctttgta
     8521 tcttcattct ccctactttc ttatgcaagc cttttctttt tattatatca cagataagta
     8581 tttctttcac aattgaattg aacttcccat ctattttcta taaatcctaa atgccataat
     8641 gctttcaatt cttgtcattt tgtgatatca tgtagaggta atgaaaggaa atgaactaat
     8701 ccacagtggc ttattccaag tataccactt gggctttggc agtagttaac tgcactaaac
     8761 ataatataag gagagaaaat gtctgcaata gaacgtatta caaaagctgc tcacttaatt
     8821 gatatgaacg atattatccg tgaagggaat cctactctac gcgcgattgc tgaggaagtc
     8881 actttccccc tatctgacca ggaaatcatc ctaggcgaaa agatgatgca attccttaaa
     8941 cattcccaag atcctgtcat ggctgaaaaa atgggactcc gcggtggtgt tggactggct
     9001 gctccccagt tagatatctc aaaacgcatt atcgctgttt tggtacctaa tattgttgaa
     9061 gaaggcgaaa ctccacagga agcctacgat ttggaaggca ttatgtacaa tccaaaaatc
     9121 gtctctcact ctgttcaaga tgctgctctt ggcgaaggag aaggttgcct gtctgttgac
     9181 cgtaacgtgc ctggctatgt tgttcgccat gcccgcgtta ctgttgacta ctttgacaaa
     9241 gatggagaaa aacaccgtat caaactcaaa ggctacaact ccattgttgt tcagcatgaa
     9301 attgaccaca ttaacggtat catgttttac gatcgcatca atgaaaaaga cccatttgca
     9361 gttaaagatg gtttactgat tcttgaataa agaaaatccc gttgcaagac ggggttttgt
     9421 gttataatag aggcatgaaa acaaatgata ttgtctatgg tgtccacgcc gttaccgaag
     9481 ccctccttgc aaatacagga aacaaactct acctccaaga agatctccga ggtaagaatg
     9541 ttgagaaagt caaggaacta gctacagaaa agaaggtgtc catttcttgg acatcaaaaa
     9601 aatctctctc tgagatgact gaaggtgctg ttcatcaagg ttttgttcta cgagtgtctg
     9661 aatttgccta tagcgagcta gattacatcc ttgcaaaaac acgccaagaa gaaaatccac
     9721 ttctattgat tctagatggt ctaaccgatc cccataatct gggttctatc ttgcgaacag
     9781 ccgatgcgac caatgtttca ggtgtcatca ttcccaagca ccgtgctgtc ggagtaactc
     9841 ctgtcgttgc caaaacagcc acaggtgcta ttgaacacgt tccaattgcc cgagtgacaa
     9901 acctcagtca aaccttagat aaactcaagg atgaaggctt ctggaccttt ggaacggata
     9961 tgaacggtac tccttgccac aagtggaata caaaagggaa aatcgccctc atcatcggaa
    10021 atgaaggaaa aggcatctct agcaacatca aaaaacaggt cgatgaaatg attaccattc
    10081 cgatgaatgg acatgttcaa agcctcaatg ccagtgttgc tgcagccatt ctcatgtacg
    10141 aagtttttct aaatagacta taaaaaagtt tccagtcatc tgattggaaa cttttttatg
    10201 attaactatg ttctgtgata aacttgtagg cttcttgacc agcgatagct ccatctccaa
    10261 ctgctgttgt tacttggcga aggtctttca agcgaacatc tccaactgca aagataccgt
    10321 cgactgcagt cttcatgtgg ctatctgtca caatccagcc tgcctgatct tggatattca
    10381 attctttaac aaaatcgcta agagggtcca aaccaacata gataaagaca ccaccgaagg
    10441 cttgctctgt cacttgacct gttttcacat tttcaaaaac gacagattct actctgtttt
    10501 cacccttgat ttccttcact acagaatccc agataaagct gattttttca ttcgcaaagg
    10561 cgcgatcttg taaaaccttt tgggcacgaa gttggtcacg acggtgaaca atggtaacag
    10621 tcttagcaaa acgagtcaag aagagggctt cttcaacagc tgaatctcca ccaccaacta
    10681 ccaataaatc ttggtcacgg aagaaagcac catcacacac agcacagtaa gaaacaccac
//
LOCUS       DAVBHS010000064        10611 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVBHS010000064 DAVBHS010000000
VERSION     DAVBHS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.064"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            110..358
                     /locus_tag="VQ651_001175"
     CDS             110..358
                     /locus_tag="VQ651_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754181.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRIYSGNLILQELITVNGAYKATYIGELSSN"
     gene            489..1661
                     /locus_tag="VQ651_001176"
     CDS             489..1661
                     /locus_tag="VQ651_001176"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HET2754182.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENADSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            1661..2935
                     /locus_tag="VQ651_001177"
     CDS             1661..2935
                     /locus_tag="VQ651_001177"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HET2754183.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLEFANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLIGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VAFSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(2971..3936)
                     /locus_tag="VQ651_001178"
     CDS             complement(2971..3936)
                     /locus_tag="VQ651_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2754184.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQTIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(3917..5371)
                     /locus_tag="VQ651_001179"
     CDS             complement(3917..5371)
                     /locus_tag="VQ651_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET2754185.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKSQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            5748..7631
                     /locus_tag="VQ651_001180"
     CDS             5748..7631
                     /locus_tag="VQ651_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2754186.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYALADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVSKGDAVIE
                     VKI"
     gene            7780..8667
                     /locus_tag="VQ651_001181"
     CDS             7780..8667
                     /locus_tag="VQ651_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2754187.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGTSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(8987..9604)
                     /locus_tag="VQ651_001182"
     CDS             complement(8987..9604)
                     /locus_tag="VQ651_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET2754188.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(10019..10207)
                     /locus_tag="VQ651_001183"
                     /pseudo
     CDS             complement(10019..10207)
                     /locus_tag="VQ651_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         3082 a         2073 c         2349 g         3107 t
ORIGIN      
        1 aatttgattg ctggcattat caatcatgaa ctaggattct agttttgcag gaagtctact
       61 actttcctta ttgtctgtaa atctactgac cttgttgttt atcccagtca tggtttctag
      121 ttcgggctca gagtttcaaa gtggatggca agagcatcaa ttgattgctg agaaggttag
      181 taaaacactt gataagacat ttgataagga tgtcagagaa attccgacca gtcagtttta
      241 tcaaaaattt gtagatgaga tgggaaggat ttactcagga aatttgatcc tccaggagct
      301 gataactgtg aatggagctt ataaagctac ttatatcggt gaactctcta gcaactaatc
      361 attctttaaa atgatgacca ataagaggct tgatttggtg aatcaagtct tttttctctc
      421 ttttactgcc atttgtgata aaatagtata gaacgatttt ttacatgaat gataaaacag
      481 aggtaaatat gacaatcggt attgataaga ttggttttgc gaccagtcaa tatgtcttga
      541 aattacaaga cttagcagaa gcgaggggaa ttgaccctga aaaattaagt aaaggactct
      601 tactcaagga attgagtatt gcgcccctaa ctgaggacat cgtgaccttg gcggccagtg
      661 ctagtgactc tattttaact gagcaagaaa gacaagaagt tgacatggtc attgtggcta
      721 ccgagtcagg aattgaccag agtaaggctg cggccgtctt tgtgcatggc ttgctgggca
      781 tccagccctt tgctcgtagt ttcgagatta aagaagcctg ctatggggcg acagctgccc
      841 tccattatgc caaattgcat gtggaaaatt ctccggagtc caaggtcttg gtcattgcca
      901 gtgatattgc caaatatggt attgaaactc cgggagaacc aactcaaggt gctggaagtg
      961 tagctatgtt gattacacaa aatccacgca tgatggcctt taataatgac aatgtagctc
     1021 agacccgtga catcatggat ttctggcgac caaattactc gacaactcct tatgtaaatg
     1081 gtgtctattc tacccaacaa tacttggata gtttgaaaac gacttggctt gaatatcaaa
     1141 aacgctacca gcttactctg gatgattttg cggctgtttg tttccacttg ccttatccta
     1201 aattagcgct aaaaggcttg aaaaaaatca tggataagag cctgcctcaa gagaaaaaag
     1261 acctcttaca aaagcatttt gaccagtcta ttctctacag tcaaaaggtg gggaatatct
     1321 acacaggttc acttttcctt ggacttttgt ctctcttgga aaatgcagat agcttgaaag
     1381 ctggggataa aatcgccctt tatagttacg gaagtggagc tgtggctgag ttcttcagtg
     1441 gtgaattggt tgaaggatat gaagcttatt tggataaaga ccgcttgaac aagctcaacc
     1501 aacgaactgc cttatccgtt gcagactatg aaaaggtctt ttttgaggaa gtaaacttgg
     1561 atgaaacaaa ctctgcccag tttgctggct atgaaaatca agattttgcc ttggttgaaa
     1621 ttctcgacca ccaacgccgt tatagcaagg ttgaaaaata atgaagataa gttggaatgg
     1681 attttctaaa aaatcatacc aagagcgcct cgagctgtta aaagctcagg cgctccttag
     1741 tcctgagaga caagctagtc tggagaagga tgaacagatg agtgtgactg tggcagacca
     1801 gctgagtgag aatgtagtgg gaactttttc tctgccttat tcgctggttc cggaggtact
     1861 tgtcaacggt caggaataca ccgttcccta tgtgacagaa gaaccctctg tggttgcggc
     1921 ggccagctat gccagcaaaa tcatcaagcg tgcaggtggt tttactgcac aagtccatca
     1981 gcgccagatg attgggcagg tagcccttta tcaagttgct aatcctaaac tagcgcaaga
     2041 gaagattgcc agcaagaaag cggagctctt ggagtttgcc aatcaagcct atccttctat
     2101 cgttaaacgt ggaggtgggg cgcgtgatct gcatgtcgag cagataaaag gcgaaccaga
     2161 ctttctcgtt gtttatattc atgtcgatac ccaggaagcc atgggtgcca atatgctcaa
     2221 caccatgctg gaagccttga aaccagtctt agaagaactc agtcagggac agagtctcat
     2281 aggaatcctg tccaactacg cgaccgattc tctggtgact gcaagctgtc gcatggcctt
     2341 tcgctacttg agccgccaaa aggatcaagg acgagagatt gcggagaaaa ttgcgttggc
     2401 tagtcagttt gcgcaggctg atccttaccg agctgctact cataataaag gaatttttaa
     2461 tggtattgat gcgattttga ttgccactgg taatgactgg cgtgccatcg aagctggggc
     2521 ccatgccttt gccagtcgag atggacgcta tcaaggtctt agttgctgga cgctggacct
     2581 tgaaagagaa gaattggtcg gtgagatgac cctgcccatg cctgtagcga ctaagggtgg
     2641 ctctatcggc ctcaacccac gtgtagcttt cagtcatgat ctactaggaa atccttctgc
     2701 cagagaatta gcccagatta tcgtgtccat cggtcttgcc caaaattttg cagccctcaa
     2761 agctttggta agtacgggca tccagcaagg ccacatgaaa ctacaggcca aatccctagc
     2821 tctcctagct ggggctagtg aatctgaagt tgctccccta gtagagcgcc tcatctcaga
     2881 taaaaccttt aacctagaga cagcccagcg ctatctcgaa aatttaagat cataaaaaac
     2941 tcagacgaat cggtctgagt tttcttgtgt ttaaatactt tttcctggta atagtgtaac
     3001 tggtaagaag taaccagttg tggcaacttc cttgccttca atcttttgca agagaagatc
     3061 aatagtgaga caagcaatct cttccaaagg ttgcttgata gtagccaatt gagggtagta
     3121 attttcgata aagtaggtcc catcatagcc gatgaccttg agctcttttg ggacagaaat
     3181 gcccaattct tgagcgattt taatgaccag aatagctgtc aaatcatccg aagcaaaaat
     3241 ggcatctggt ttttcccggg tcaagatatt cttgatttcc atttcttttc tgacgggaga
     3301 aaagtcactg gaaacattga taataggagc ttttgggagt acggatgcaa aaccagcgtg
     3361 gcgtagtccg gttggcgaat tggaattgtc attccctgta atcatgatga tagtctgggc
     3421 acctgtcttg accaaggttt gggcagcaag aaccccacca gcatagttgt cagaggagac
     3481 gacagggatg tctggcgata ggtttcggtc aaaggaaata atcggcgctg tcacacgatt
     3541 gtagtcttcg attcctaggt tgtgactacc agaaatgatg ccgtccacct gattggcttc
     3601 caacatttcg atgtattcgc gttccttctc agaatcatgt tcactgttgc agatgatggt
     3661 cttgtaacca tttttgaaga gttggtgttc caatttatca atcaattctg catagaaaac
     3721 attggaaata ttagggaaaa tcaagccgat taacttagct gattttcctt gcagactacg
     3781 agccaggttg ttgggtttat agcccaattc tcgcatggct tcattgactt tttggatggt
     3841 tttctcagat agataccctt ttttattgat aacccgagaa acggtagtag gactgacgcc
     3901 tgcaagtttg gcgacatcag ttagttttgc gaccataatc taattcatag taagttccag
     3961 ttgggtttcc agatttgatc aggataccat tttggtccgc atgtgggaag acacgaccag
     4021 aaaatacttt ttctccttta ttgatgaaaa tttcaaagac agagttatcg atgaagattg
     4081 tagcagtagt agcctgattc tcgatagggc aagaacgagt tgtcccaaat tcttgggcat
     4141 actgttctcc agcctggcta cgatccactg tcacttgacc gtttacaagg tcaaagttga
     4201 ttgaaagtcc cttaccttct ttatcagcaa gtaagacaat ctcgctctgg ctattagctt
     4261 ccaagttgag ttcaagttcg taagtgttct tggtttggga acggtttgag aaggcttctt
     4321 cagaagcacg aaggtcctta atagcagcga ctggatactg gtagagcttg tcgtctttga
     4381 tagtgagttc cttgaccaaa gagaaggttc cttggtggtc aaaacggtca gatgggtaag
     4441 aaacatctgg caaaccaagc cagctaactg ctagagcacg cccatcagga gcgttgaagg
     4501 cttgagttgc ataggcttcg aaaccgtaat ccatgttttg aagttgagac acatctacca
     4561 ttttggcatt tttagggtca aaggaagccc caatcttata catatttgga aagatattat
     4621 cgtagtctag aactttctta tccaatcctt gtggacagta gagaaggaca ggatgttcct
     4681 ctacaaagac cagattagga cattccatca tgtaggcagt acggtcgtta gcaaagtcaa
     4741 ggtcgccaac tgcttgccag tttgtgtagt cgttattgac agccttgtag agacggacga
     4801 agcctttttt ctccaagtcc tgtccaccga cgacagcata gtattgactt ttaaagttaa
     4861 aaatttgtgg atcgcggaag tggtcagtag agtctgctgg ctggtcaatc aagatcttgt
     4921 caatctttgt aatcttaccc tccttgtcca tcaaagcacc gatctggtat gggtgacgga
     4981 tccagttttc atcgcgaaca tttcctgtat aaaataggaa taagttatcg ccaaattgca
     5041 tggcagaacc agagtaggca ccgtggctat ctaatggagt atctggtaaa actttgattc
     5101 cagtttcttt aaagtgaacc aaatcatcac tttctagctg tgcccaagat ttcaaaccgt
     5161 gggctgcacc aaaaggaaaa ttctggtaaa agaggatcca cttgccatca aagtaagaaa
     5221 agccatttgg gtcgttgaga agtcctgttt ttggctcaac atggtaatga gtatgccatg
     5281 gagattgtgc catattttcc tttatatgtt gaatttcttc ttgcgtccaa tcttgataaa
     5341 gtctgtaacg acgctcagtt gtccattcca ttcattcctc caaaaatctt atcactttta
     5401 atagtaactg ttttcgataa aaaatgcaag ccttttatgt taaaaaagag gaaaaactgt
     5461 caaacgatta tcataaaata aatacaggaa atcaatcaaa tttttacgaa aatgtgaaag
     5521 ataatgaaat aaaagacttt ttaaccaagg tttcaaactt atttttcaag gaattcctga
     5581 tgaaacagtc aaaaacaatc aatctaaaac ttgcccacta agtggataga atattttttc
     5641 tgcaaaacgc ttgacatatt tttaaaacat gataaaataa tagtcgtaag ggcgaataaa
     5701 atcgctttca aacaagaaca aaatgttata taaggagatt tttgcaaatg aacaatcagg
     5761 aaattgcaaa aaaagtcatc gatgccttgg gcggacgtga aaatgtcaat agtgttgccc
     5821 actgtgcgac tcgtctacgt gtcatggtca aagatgaaga gaaaatcaat aaagaagtga
     5881 ttgagaactt ggaaaaagtt caaggtgctt tctttaactc agggcaatac caaattatct
     5941 ttggtacagg tacagttaac aaaatgtacg atgaagttgt tgtacttggc ttaccaacat
     6001 catctaagga tgacatgaaa gcagaagttg ctaaacaagg gaactggttc caacgtgcta
     6061 tccgtacttt cggtgatgtt ttcgttccaa tcatcccagt tatcgtagcg acaggtctct
     6121 tcatgggtgt gcgtggtctt ttcaacgctc ttgaaatgcc acttccaggt gactttgcaa
     6181 cttacacaca aatcttgaca gatacagcct tcatcatctt gccaggtttg gttgtgtggt
     6241 caaccttccg tgtatttggt ggaaatcctg ccgttggtat cgttcttggt atgatgcttg
     6301 tctctggctc acttccaaac gcttgggcag ttgctcaagg tggtgaagta acagcgatga
     6361 acttctttgg tttcatccct gttgttggtt tgcaaggttc cgttcttcca gccttcatca
     6421 tcggggttgt cggagctaaa tttgaaaaag ctgtccgcaa ggttgttcca gatgtcattg
     6481 acctcttggt aacaccattc gtgacacttt tggtcatgtc tatccttgga ctctttgtca
     6541 ttggaccagt tttccacgtt gttgaaaact acatccttat tgctacaaaa gcgattctta
     6601 gcatgccatt tggtcttggt ggtttcttga ttggtggggt tcaccaattg atcgtcgtgt
     6661 caggtgtgca ccacatcttc aacttgcttg aagtgcaatt acttgctgct gaccatgcta
     6721 acccattcaa cgctatcatc acagctgcta tgacagctca aggtgctgct actgttgcgg
     6781 ttggtgttaa aacaaaaaat ccaaaactga aaacacttgc tttcccggct gctctttctg
     6841 ccttcctagg tattacagag cctgctatct tcggggtgaa cttgcgcttc cgtaaaccat
     6901 tcttcctttc attgattgct ggtgcaatcg gtggtggatt ggcttctatc cttggacttg
     6961 ctggtactgg taatggtatc accatcatcc ctggtacaat gctttatgtt ggtaacggac
     7021 aacttccaca ataccttctt atggtagctg tatcatttgc ccttggtttt gctcttactt
     7081 acatgtttgg ttacgaagat gaagtagacg caactgcagc tgcaaaacaa gctgaagtgg
     7141 ctgaagaaaa agaagaagtt gcgccagcag ctcttcaaaa tgaaacactt gtaactccta
     7201 tcgtcggtga tgttgtcgct cttgctgatg tcaatgaccc agtcttctca agtggagcta
     7261 tgggacaagg tatcgctgtg aaaccaagtc aaggcgtggt ctatgcacta gctgatgctg
     7321 aagtttcaat tgcctttcca acagggcacg cttttggttt gaaaacaaga aatggtgctg
     7381 aagttttgat tcacgttggt attgacactg tatctatgaa cggtgacggt tttgaagcaa
     7441 aagttgctca aggtaataag gtgaaagctg gcgatgttct tggaacattt gactcaaaca
     7501 aaatcgctgc agctggactt gatgatacaa caatggttat cgttacaaat acagctgact
     7561 acgcttcagt agctccagtc gcaacaggtt cagtttcgaa gggggatgct gtgatcgaag
     7621 tgaaaatcta atcaatcctc tctaatgtga aaacgaacaa atgacatgtt tgttcgtttt
     7681 tattagatag taagatttac agaggaaaat ctaaaaaata gagacattta gactttcgaa
     7741 gtatgctata ataaagaaaa taaaaacaag aggtttatca tgacaaaatt atatggaagc
     7801 ttggaagcgg gcggtacaaa gtttgtctgt gctgtcggtg atgaaaactt taacgttgta
     7861 gaaaaaacac aatttccaac aacaactcca atcgaaacaa tcgataaaac cattgagttc
     7921 ttctcaaaat tcgataacct tgctggtctt gcagttggtt catttgggcc gattgatatt
     7981 gacaaaaact caaaaactta tggctttatc acgacgactc caaaaccaaa ctgggcaaat
     8041 gtggacttgc ttggtgccct tcgtcgcgcc ctaaacgtgc caatgtactt cacaacagac
     8101 gtaaacagct ctgcttacgg tgaaatggtt gctcgtaaca atgctggtgg tcgtatcgaa
     8161 aacttggttt actacacaat cggtacaggt atcggtgcag gcgtcatcca acgtggtgag
     8221 tttatcggtg gtgtgggtca ccctgaaatg ggtcattatt atgttgctag acacccaatg
     8281 gatattgaaa aagagtttaa gggtgtttgt cctttccata agggatgtct agaaggttat
     8341 gcagctggta caagtttgga agctcgtaca ggtgtacgtg gggaaactat tgaactcaac
     8401 aaccctgttt gggatgttca agcctactat atcgctcaag ctgcggttaa tgcgacagtg
     8461 actttccgcc cagacgtgat tgtctttggt ggaggggtca tggctcaaca acatatgctg
     8521 gaccgtgtcc gtgagaaatt tacatctctt cttaatggtt acctaccagt accagatgtg
     8581 cgtgactata tcgtgacgcc agcagtcgca ggaaatggtt ctgccacact tgggaacttt
     8641 gttcttgcaa aagaagtttc aaaataaagc acaaaatccg cttgggaaac caaacggatt
     8701 ttgtggttga aaacaatatt tttgagcctt tccttaagtg attacggacg gtagcgactt
     8761 tcttcgaaat tccataccta aactttgagc ctagtgtctc aaagtttccg aatacctgaa
     8821 accattagat ttcaggtgtt tctatcacgg cggaaaggct aggtaagtcc tcgcctcact
     8881 atcgaaaaat atgcataatt tccataccaa agtatattta gtatcaagct atagttaatc
     8941 tacgctttaa aaatccactt gggaaaccaa acggattttt tacgtgtcaa agcatttgcc
     9001 agaaaaagtc aataatatag gtcagaccgt aggtataaac cacgagggta aagggtttgg
     9061 tcagaatgat gatagtcatg tagcgcttga aactcatctt ggtcagggcg gccagcatac
     9121 agagaaagtc agctgggcta atgggccaaa tcatcataaa aataaagaag cggtcaaaac
     9181 gattgccctt atctagccag ccgatgtact tgtcgtaggt gcgcttgctg acgacagact
     9241 ggacaaaggc agctccgtat aggcgcacta gataaaagat aatggcacag ccaatcacga
     9301 tgccgatata gttgtagata gtcccgatga tgtgcccgta gataaagacc ccagccaccg
     9361 aggtcaaggc ccctggaatg atagggacga cagtctgtaa aatctgtaaa aagataaaga
     9421 gaggtggacc ccagatgcct gcctgctgga taaaggcaga gagggtttcc ttggattgta
     9481 aaatcccagc ctgataagcc caaatacaga aaatcaaact cccaacccca ccgacaatcg
     9541 atgagatatt gataactcgc tgcagaaggg gagaaacagc tttcatttgt ttatcctgtg
     9601 acatgtaaac tcctcataga tagtatattt ctactatacc atattttgga ggagaaaggg
     9661 ggaatgagtc ttttttgata aggaatagaa agtaaatagc ccttcgagac ttagaatagg
     9721 tctgtcaact aaaggttata tgcattctag gtttcgaata atcgcaattc cataaattta
     9781 aaagtattct aagtttcgaa tagcttatct gagataattt tgaggcattc gcgctttcga
     9841 atagtcattt tccctaaagt ttatatgtat tctgaagcta gaaagactaa aatataaaag
     9901 tagtaagagt ctctctctga tggacgtaat ttatggctag tatgaatcct ttgaattgtt
     9961 agttaatttt aatattaatc agttctgaaa taattactaa atatagttag atggtgagtt
    10021 aattattgct aaatttcaag ttcaagttag tcatccagaa gtcttctctg ggggacttgt
    10081 agttcaagca ttttttagga tagttgttaa tccacttttc gataaatgcg acttcctttg
    10141 tagttgtttt cttagttccc tgaggtaacc atctatgaat gagcctgttg tgattttcat
    10201 tagatcctat agtgatgtgc ataatagata tagcagattg aaactagaat agtatacatc
    10261 tacttctaaa acattgttag aaatcaattt gactgtcctg atcaatttgt cctgttttta
    10321 tttcatttta ctatataatc cttagaaaac atatcggaca agcggttgaa ttccgttccg
    10381 tccattagta gataaaggat tactccttct tgaaatgaca gatgaacttg tttcttataa
    10441 aaatgttgtc tgaaactatt agcctttttc cagaagtttt agaggaagaa aactttacga
    10501 ggaaaaagga gttactcaaa taacaattca ggattaaaaa tagacagttg aggagcggaa
    10561 ggtataaatt aagtttgcta ctgtataatg gatttatcac taatactctt c
//
LOCUS       DAVBHS010000065        10477 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVBHS010000065 DAVBHS010000000
VERSION     DAVBHS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10477
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.065"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(32..985)
                     /locus_tag="VQ651_001184"
     CDS             complement(32..985)
                     /locus_tag="VQ651_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET2754189.1"
                     /translation="MSISQRTTKLILATCLACLFAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(1053..2262)
                     /gene="sstT"
                     /locus_tag="VQ651_001185"
                     /pseudo
     CDS             complement(1053..2262)
                     /gene="sstT"
                     /locus_tag="VQ651_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            2401..2961
                     /locus_tag="VQ651_001186"
     CDS             2401..2961
                     /locus_tag="VQ651_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HET2754190.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            3018..3926
                     /locus_tag="VQ651_001187"
     CDS             3018..3926
                     /locus_tag="VQ651_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET2754191.1"
                     /translation="MVLEKQLGHGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            3878..4468
                     /locus_tag="VQ651_001188"
     CDS             3878..4468
                     /locus_tag="VQ651_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HET2754192.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            4471..5577
                     /gene="yqeH"
                     /locus_tag="VQ651_001189"
     CDS             4471..5577
                     /gene="yqeH"
                     /locus_tag="VQ651_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HET2754193.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVATRKAII"
     gene            5791..6102
                     /gene="yhbY"
                     /locus_tag="VQ651_001190"
     CDS             5791..6102
                     /gene="yhbY"
                     /locus_tag="VQ651_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HET2754194.1"
                     /translation="MSLASKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            6154..6783
                     /locus_tag="VQ651_001191"
     CDS             6154..6783
                     /locus_tag="VQ651_001191"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET2754195.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            6783..7376
                     /gene="yqeK"
                     /locus_tag="VQ651_001192"
     CDS             6783..7376
                     /gene="yqeK"
                     /locus_tag="VQ651_001192"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HET2754196.1"
                     /translation="MSYQDYINCSREALLEKMAEFLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSNQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIANLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            7377..7877
                     /locus_tag="VQ651_001193"
     CDS             7377..7877
                     /locus_tag="VQ651_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET2754197.1"
                     /translation="MKTAFIIIDVQNILVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            7890..8243
                     /gene="rsfS"
                     /locus_tag="VQ651_001194"
     CDS             7890..8243
                     /gene="rsfS"
                     /locus_tag="VQ651_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET2754198.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            8563..9303
                     /locus_tag="VQ651_001195"
     CDS             8563..9303
                     /locus_tag="VQ651_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2754199.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            9313..10410
                     /locus_tag="VQ651_001196"
     CDS             9313..10410
                     /locus_tag="VQ651_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET2754200.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVLD
                     YQKIADLYTEKGAEMEKFVKNLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKVRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
BASE COUNT         3148 a         2065 c         2434 g         2830 t
ORIGIN      
        1 gcaaggcgac gctgacgtgg tttgaagagt attactgtct atatttttgg taaaaatcaa
       61 cttttacttg gatgaaggtt ttggcttcac gtaggagttg aagaagggtg gcgcgggttt
      121 caaattcttc tcttgtcttg ggcagactgc ggttccggaa gacttccaga taacgttcaa
      181 tttcatctag caaatcagaa gcaggattgg tctggctcag ttgacctgca atttttgaaa
      241 agagttgcgc taagatcagg ctttcactgg cggcaaggtg acaagtgtta atctgttggg
      301 ccatgtttct caggatacga ctttgtcgtt gtctcatctc aaagtagtgg atatggtagt
      361 cggtctggtg aaagaggtgg tcagagtgat ccaaatagac cagtctgagg gcttctttca
      421 aaagcgtgtc taattctgct accagctgtg ctcggttgcg tccgtctcct ctggataaat
      481 agtatttgaa gcgctggagg atatctttta acttttcttc caccagcgtg tggtagtgct
      541 ggatttcctc ttctcgtgaa ggcatataga gattaacaag caaggcaaat cctgtaccga
      601 tagcaaagag aaggaattcg ttgactagga ggtctggaga ggttgactct tgaaccaaga
      661 gatggctaac caaaaccgtg cttggtgtga tgccaatttc ccagcccatc ttgtaggcta
      721 aaggaacgta gaaggccaga tagaggccga gactccagat atgaaatccg ctcaagtgaa
      781 aagctagaac accgatagcc agagctagaa gcatagaaaa aagacgattg cgagccagtt
      841 ttaaagtact tctacgcgta tcagataggc tcaagagagc gataattcca gccgaaactg
      901 ctgacgaaag attgagaaaa taagcaaaca ggcaggcaag acaggtagct aagatgagct
      961 tggtcgtacg ttggctaata gacataagaa tttcctaata agttagaata aaagcgtaaa
     1021 agacaagaca tgagcaggct tgccttgatg agttattttt tacgggttgc tgcgtattcg
     1081 gcaacggcgg taaagaggac atctgtagaa gagttaaggg ctgtttcaca tgagtcttgg
     1141 atgacaccaa tcacaaaacc aaccccaaca atttgtatgg caatatcgtt agaaataccg
     1201 aaaaggctac aagcaactgg gataagaagg agggaacctc cggcaatacc tgaagcacca
     1261 caggctgaga tagctgctac cacactgagg acaaaggctg tggcaaagtc aacaggaatt
     1321 ccaagagtgt taactgtaac aagggtcaaa aggttaatgg taatcgctac tccagccata
     1381 ttgatagtag aaccgagtgg gatagaaaca gaataggtat ctgggttgag tccaaggtca
     1441 tggcagagtt tcatattgac aggaatgtta gccgcagaac tacgagtgaa aaaggctgtc
     1501 acaccgctga cacggaggca gttccaaact agagggtaag gattgcgtct cataaagaag
     1561 aaggcaatca aagggttgac cacaggggca acaaaaagca tagtcgttac taatagaacc
     1621 aataaaatac cgtagttggc aagccttccg actcccttgt cagaaatggt tttaaaaaca
     1681 agaccaagga ttccaaatgg agtcagattg atgatccatt cgacaatttt agaagtcacg
     1741 tcagcgatag tttttagcaa ttctttacta tttttactgg cttctctcat agcgattcca
     1801 aaaatgactg cccaagataa gattccaata tagttagcag taagcagggc gttgactggg
     1861 ctgtcaacca gtttgagcaa gaggttgctg aggacctgcc caatcccatc tggtggtgca
     1921 atttcagtat tggcactatt tagggtaatt tcaataggga cgatgaaact tgctagtaca
     1981 gctacaagag cagcggcgaa agtccctatc ataggatata caagaaaaca acagttttca
     2041 tattgctatc ttgtcccttt tgatgttggg aaagggcatt ggcaacgaga gcaaagacta
     2101 ggataggagc aacagctttt agacctccaa cgaataaatc cccgagtagc ccaatccctg
     2161 agagattagg aagggtcagt cctaggattc cccacaaagc ataccaatca agatacgctt
     2221 gacaaggctt gccttattcc aagcatgaat gattcttttc ataataatct cctttttgtg
     2281 tagtgattat gattatagta taaatgatag acaaaatcaa gaattttctg tctatttttt
     2341 gaatatttat ggagaatgag actgatgaaa atatggtata atgaaataaa ggagttttat
     2401 atgcaaaaat ttattcaggc ttatattgaa aagctagatg tgacaaccat tatcgagaat
     2461 attctaacca aggtcatttc tcttttactg cttttgattg tattttatat tgctaaaaaa
     2521 atgcttcata ccatggtgca gagaattgtc aaaccttctc taaaaatgtc tcgtcatgat
     2581 gtcggacgcc aaaaaaccat ctcacgttta ctagaaaatg tgtttaatta tacgctatat
     2641 ttctttttac tctactgcat tttgtcgatt ttaggtttgc cagtttctag tttgctggct
     2701 ggagctggta ttgctggggt agcgattggt atgggagccc aaggctttct gtctgatgtc
     2761 atcaatggct ttttcatcct ctttgaacgt caactggatg tgggagatga ggtcgttctg
     2821 acaaatggac cgattactgt atcgggtaag gttgtcagtg tgggaattcg tacgacacag
     2881 cttcgtagcg aggagcaagc ccttcacttt gtccctaacc gaaatatcac agttgttagc
     2941 aatttctcac gcacagacta gacctgttat tttaagtaat ttgtggtaca atagagggag
     3001 tttaataagg agaaaagatg gttttagaaa agcagttggg ccatggttgt acctggatag
     3061 acctagacct aggaaagttg aataaactag aagacctttc tgaaatttac ggtttggaca
     3121 aggaaaccat tgaatacgca ctggatagaa acgagcgcgc ccacatggac taccaccgtg
     3181 aaagtgggac ggttaccttt atctataatg tcctagacgt aaaaaaggac aaggcctact
     3241 atgagacttt tcccatgacc tttatcgtcg agcatcgtcg cctgattacc attagtaata
     3301 ccaagaacgc ctatgtcatt gaacagatga ctcgttatct ggagagccat gacacgcttt
     3361 cgatttataa gtttctcttt gccagtctgg aaatcatcag caatgcctac tatcctgtca
     3421 ttgagcagat ggacaagagt agggatgagg tcaatgacct cttgcgccag cgaactacca
     3481 agaaaaacct ctttgccctg tctgatttgg agactggtat ggtttatctg acggcagctg
     3541 ccaaacaaaa tcggattttg ttagagcata ttcaaggtca tgccttgtat cgtagttttg
     3601 atgagattga gagagaacag tttgatgatg ccatgattga ggctcatcag ctggtatcca
     3661 tgacagacct aatctctcag attttacagc agctttcagc ctcttacaac aatattctaa
     3721 acaataatct gaatgacaat ttgacaacct tgactatcat ttcagtcttg ctagctgttt
     3781 tggcagtcgt gacaggcttt ttcggaatga atgttccctt acctttaaca gatgagcccc
     3841 atgcttggct ctatatcagt ttggctagtg caggtttgtg gattgtttta tccttgttac
     3901 taaggaaaat tgcgaaaaaa agttaagaaa aggagccaga atggcgattg aaaattatat
     3961 accagatttt gctgtggaag cagtctatga tctgacagtc ccaagcctgc aggcgcaggg
     4021 aatcaaggct gttttggtcg atttggataa taccctcatt gcttggaaca accctgatgg
     4081 aacgccagag atgaagcaat ggctacatga ccttcgggac gcgggtattg gcattatcgt
     4141 agtgtcaaat aacaccaaaa aacgcgttca acgagcagtt gagaaatttg ggattgatta
     4201 cgtttactgg gccttgaagc ccttcacatt tggtattgac cgtgccatga aggaattcca
     4261 ctatgacaaa aaggaagtgg tcatggttgg cgaccagctc atgacagata tacgagcagc
     4321 ccaccgtgca gggattcggt caattttagt caaacccttg gtccaacatg actcaatcaa
     4381 aacgcagatt aaccgaactc gtgagcgtcg tgttatgaga aaaatcactg aaaagtacgg
     4441 accgattaca tataaaaaag gaatttaact atggaagaaa ttctctgtat tggttgtgga
     4501 gcaaccattc agacgacaga taaagctggt cttggtttta ccccccagtc ggcacttgaa
     4561 aaaggtttgg agactggcga agtctattgc caacgctgtt tccgtctccg ccactacaat
     4621 gaaatcacag atgtccagtt gacgaacgat gatttcctca agctcttgca cgaggtggga
     4681 gacagtgatg ccttagtggt caatgtcatt gatatctttg attttaatgg atctgtcatc
     4741 ccaggtttac cacgtttcgt ctcgggcaat gatgtcctct tggtaggaaa taaaaaagat
     4801 atccttccta agtcagttaa gtctggtaag attagccagt ggctcatgaa acgtgcccat
     4861 gaagaaggtc ttcgtccagt cgatgtggtc ctaacttcag cacaaaataa acatgccatt
     4921 aaggaagtca ttgataagat tgaacactac cgtaagggcc gcgatgtcta tgtggtcggt
     4981 gtgaccaacg ttggaaaatc aactctaatc aatgctatta tccaagaaat cacgggtgat
     5041 cagaatgtca tcactacttc acgcttccca gggacaacct tggacaaaat agagattccg
     5101 cttgacgacg gatcttatat ttacgatacg ccgggaatta tccaccgcca ccagatggct
     5161 cactacttga cggccaaaaa cctcaagtat gtcagtccta aaaaggaaat caagcctaag
     5221 acctatcagc ttaatcctga gcaaacccta tttttaggtg gtttgggacg ctttgacttt
     5281 atagcaggag aaaagcaagg atttactgct ttctttgata atgaactcaa actccatcgt
     5341 agcaagcttg aaggagctag tgctttctac gataagcacc tgggaactct tctgacacca
     5401 ccaaatagca aggaaaaaga agatttccca aggctagtcc agcatgtctt taccattaaa
     5461 gataagacag acctagtcat ttcaggccta ggctggattc gtgtaacagg cacagcaaaa
     5521 gtcgccgtct gggcaccaga aggcgtcgcc gtcgccacac gaaaagcaat tatttaaaac
     5581 aaaatcgctg agcgaacaaa gtgagctcat atagcgatag ttattgccgt ggtgtgattg
     5641 ccaatctgta gattggcaag gggcacaatt gagaccaagg ctcaattgtg aggtcaggaa
     5701 atccattttt caaagatgaa atctttggaa aattagttcc gaccgtccct caccacctaa
     5761 aataactatc aaaaaacaag gaagaaaatt atgtcattag catcaaaaca acgtgccttc
     5821 ctcaacagcc aggcacacac cctcaaacct atcatccaaa tcgggaaaaa tggactcaac
     5881 gaccaaatca aaaccagcgt ccgtcaagct cttgatgcgc gtgaattaat caaggttact
     5941 ctcttacaaa acacagatga aaacatccac gaagtagctg aaattttgga agaagaaatc
     6001 ggtgtggata cagtccaaaa aataggacgc atcttgattt tgtttaagca atctagcaag
     6061 aaagaaaatc gcaagatttc taagaaagtc aaagaaatct aagaccgaaa ctccaaataa
     6121 ctgtttttat agagaaataa aggggactag cctatgacaa tcgaactatt gactcccttt
     6181 accaaggtag agttggagcc agaaatcaag gagaaaaaac gcaaacaagt tgggatttta
     6241 ggggggaatt ttaaccctgt tcacaatgcc catctcattg ttgcggatca agtacggcaa
     6301 cagttgggac tggatcaagt tcttctcatg cctgaatacc aacctcctca cgttgataaa
     6361 aaggaaacca tccctgaaca ccatcgtctc aagatgcttg agttggcaat tgagggaatt
     6421 gacggcctag tcattgaaac cattgagttg gagcgcaagg gtatttccta cacctacgac
     6481 accatgaaga ttttgacaga gaagaatcca gatacggatt attactttat catcggtgcc
     6541 gacatggttg actatctgcc taagtggtac cgaattgatg aactggttga catggttcag
     6601 tttgtggggg ttcagcgtcc acgctacaag gtagggactt cctatccagt tatctgggtg
     6661 gacgtaccgc tcatggatat ctcgtccagc atggtgcggg acttccttgc ccaaggtcgg
     6721 aaacccaact ttctcctacc tcagccagtg ctagactaca tcgagaagga ggggctctac
     6781 tgatgtccta tcaagactat atcaactgct cccgtgaggc cttgttggaa aaaatggcag
     6841 agtttctacc tgagaaacgt ctaacccatt gtctaggtgt ggagcgtgcc gctatggaat
     6901 tagctcagcg ctttggtgta gatgttgaga aagcaagtct agcaggtctt cttcatgact
     6961 atgctaaaaa gttgtcaaat caggaatttc tagccttgat tgaccgttac cagctagacc
     7021 ctgacctcaa aaactggggc aataatgtct ggcatggtat ggtcggaatt tacaaaattc
     7081 aggaagattt ggatttgcat gattcagaaa tcctgcgagc cattgaagtc catacagtcg
     7141 gtgctggtca aatgacagac ctagataagg tcatctacgt cgcagactat atcgagcaca
     7201 atcgagcctt tcctggagta gatgtggcgc gtgagattgc aaatctatcg ctcaataagg
     7261 cggtggccta cgaaacagct cgtaccgtgg agtatctagc tcatcaggga ttccccatct
     7321 atccccaaac ccttgaaacc tataacgcct ttgtgcacta tttgaaagag aactaaatga
     7381 agacggcttt tataatcatt gatgttcaga atattttagt agaaactggg ttccagacaa
     7441 aaagtctatt gggaaaaatt tcttatttac aaaaccaggc tagaagcaag aatattgaaa
     7501 ttatctatgt tcaacatatt gagaactctg aagctcaaac atcagaagat tggcagttat
     7561 ctgcgctttt aaatcgaaaa cctgctgaaa aggtctttca gaagaagtat aacagtattt
     7621 tcaaagaaac tggcttaaaa gaatacttgg ataagcaggg gattgaaaaa ttagttttat
     7681 gtggtatgca gacagaatat tgtgtggata cctctgtcaa ggttgccttt gaatatggct
     7741 atcagcttat tgttccagaa ggtgctgtca caacctttga tggggatgac attccagcag
     7801 aaacgattaa tgaattttat gaggacattt gggaggagcg ctttgcagat gttctagatt
     7861 acaaacatat tttctaaaaa gaggattaaa tgaacgaaaa agaattacta gaactagtcg
     7921 tgaaagcggc tgatgagaaa cgtgcggagg atatcctcgc acttgatgta caagatttga
     7981 ctagtgtgac ggactacttt gtcatcacta gctcaatgaa tagccgtcag ttggacgcta
     8041 tcgcagctaa tatccgtgaa aaagtagctc aagcaggctt taaaggtagc catgtcgaag
     8101 gcgatgcagc tggaggctgg gtcttactgg accttggtgc tgttgtcgtg catatctttt
     8161 cagaagaaat gcgtgcccat tataatctag agaagctatg gcatgaggcg aattcagtag
     8221 atatttcaga agctcttgct tagaagatga actcagttgt agtcaactgg gtttctttgc
     8281 ttattttaga aaaattgata gaaaggtaaa gggcctcgtg tttgtttcat ttcgaaactg
     8341 aacacgagct aaaagctgag ataaaaagta actttctttg tatctgacga tagggaagaa
     8401 agtttcctat gtactctttg cttttaaggc tctttgtatc ttagaaagga tttgaacccg
     8461 tcctgtaatt ctcggaaaat agatagcctt ccttggagct cggctccatc gtaaggctcc
     8521 taattttcta tcgaattacg ggcggacttg gtatcatgtt ttatggcgac ttatgaaacc
     8581 tttgcggcgg tctatgatgc ggtcatggac gatagtttat acgacaaatg gacgaatttt
     8641 tctctgcgtc atttgcctaa gaccaaggag agaaagaaac tcttggaatt ggcttgtggg
     8701 acaggaattc aatcagtgcg cttttctcag gctggttttg atgtgactgg acttgacttg
     8761 agtgcggata tgttgaagat tgcggagaag agagcaactt cagccaagca aaagattgct
     8821 tttatagaag gtaatatgct gaatttgtcc aaggcaggga aatacgattt tgtcacgtgt
     8881 tattcggact ctatctgcta tatgcaggat gaggtggaag taggggacgt ctttaaggac
     8941 gtgtacaatg cgctgaatga agagggagtt ttcatctttg acgtgcactc gacctaccag
     9001 acagatgaag tattccctgg ctattcctac catgaaaatg cggaagattt tgccatgctt
     9061 tgggatacat atgaggacga agttcctcac tccatcgtgc atgagctgac tttctttatc
     9121 aaggaggctg acggttcctt tagtcgccac gatgaagtac atgaggagag gacctatgag
     9181 atcttgacct atgatatctt gctggaacag gctggattta agtccttcaa actctatgca
     9241 gactttgagg ataaggagcc aacagaaact agcacccgtt ggttttttgt ggcgcagaag
     9301 taggagatta gcatgaccat aacaggtatt atcgcggagt tcaatccttt tcataatggc
     9361 cataaatacc tgctggatca ggcagaggga ctgaaaattg ttgccatgtc agggaatttc
     9421 atgcagcgtg gagagcctgc tattgtggat aagtggacaa gggcccagat ggcgctggaa
     9481 aatggcgcag acttggtagt ggaattgccc tttttagtca gtgttcaggc agctgatttc
     9541 tttggtcaag gagctgtgga tatcttggat cgcttgggca ttgatagtct agtttttggg
     9601 acagaggaag ttctggatta ccagaaaatc gctgacttat acacagagaa aggtgctgag
     9661 atggagaaat ttgtgaaaaa tctgcctgat tccctttcct atccccagaa aacccaagct
     9721 atgtggaagg aatttgctgg tcttgatttt tcaggtaata cccccaatca tgttcttgct
     9781 ctggcctatg ccaaggcggt tgcaggacga aatatcaagc tgcatccgat tcagcgtcag
     9841 ggggcaggtt accattctgt gaataaagat gtggactttg cctcggcgac agccctccgt
     9901 caacatcaga aggaccaaga tttcttagaa cgttttatgc cttctgttgc cctatttgag
     9961 caggccagta aggtgatctg ggaagactat tttcccttgc tccgctatca aatcttgtca
    10021 aatcccgacc taaccaccat ctatcaggtc aatcaagaaa tggctgtgcg cattaaagaa
    10081 gctattaaaa cagcccagtc tgtggaagaa ttggtcgagt tagtgactac caaacgttac
    10141 accaaggcgc gtgtcagacg cctattgacc tatattttgg tacaggctag agaaaatgtc
    10201 ttgccagaag ccattcatgt ccttggattt actgaaaaag tcaggcaaca tctcaagtct
    10261 ctgaaaggac aggtcaatct agtcagccga attggcaaag aaccttggga tgctatgacc
    10321 caaaaagcag accagattta ccaactaggg aagccaagta tagcagagca aaattttgga
    10381 agagtgccga ttagaataga aacaaactaa gtctaccgaa aggtaggctt tttgagattt
    10441 tttacgaata atactcttcg aaaatcaaat tcaaatc
//
LOCUS       DAVBHS010000066        10464 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVBHS010000066 DAVBHS010000000
VERSION     DAVBHS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.066"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            102..1127
                     /locus_tag="VQ651_001197"
     CDS             102..1127
                     /locus_tag="VQ651_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HET2754201.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            1117..2124
                     /locus_tag="VQ651_001198"
     CDS             1117..2124
                     /locus_tag="VQ651_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2754202.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLATFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            2124..3131
                     /locus_tag="VQ651_001199"
     CDS             2124..3131
                     /locus_tag="VQ651_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2754203.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            3131..3925
                     /locus_tag="VQ651_001200"
     CDS             3131..3925
                     /locus_tag="VQ651_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754204.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
     gene            4313..4702
                     /locus_tag="VQ651_001201"
     CDS             4313..4702
                     /locus_tag="VQ651_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2754205.1"
                     /translation="MPKRYLLDGKTPRISATNIDNGILGYYEDIDDKNYRVFENFISV
                     SFLGAVFYHKYKASLDMKIHCLKLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTV
                     LPDIKIKLPIDSRGTPDWNYMERYRDR"
     gene            4668..4982
                     /locus_tag="VQ651_001202"
     CDS             4668..4982
                     /locus_tag="VQ651_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754206.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            5666..5827
                     /locus_tag="VQ651_001203"
     CDS             5666..5827
                     /locus_tag="VQ651_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754207.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            5919..7598
                     /locus_tag="VQ651_001204"
     CDS             5919..7598
                     /locus_tag="VQ651_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET2754208.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCNHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKVDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            7866..8432
                     /locus_tag="VQ651_001205"
     CDS             7866..8432
                     /locus_tag="VQ651_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754209.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKTYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            8754..8942
                     /locus_tag="VQ651_001206"
     CDS             8754..8942
                     /locus_tag="VQ651_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET2754210.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            9004..9762
                     /locus_tag="VQ651_001207"
     CDS             9004..9762
                     /locus_tag="VQ651_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET2754211.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYALS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVVF"
     gene            9820..>10464
                     /locus_tag="VQ651_001208"
     CDS             9820..>10464
                     /locus_tag="VQ651_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET2754212.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNLTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAII"
BASE COUNT         3873 a         1416 c         1984 g         3191 t
ORIGIN      
        1 ccttttttga aaaaattata ttattaaatt tgtaactgta tctattgaca atgataatta
       61 ttatcgatac aatagacttg aaatatgttt aaggagtttt tatgaaaaac aaattttttc
      121 taatagctat tttagctatg tgtatagttt ttagcgcttg ttcttctaat tctgttaaaa
      181 atgaagaaaa tacttctaaa gagcatgcgc ctgataaaat agttttagat catgctttcg
      241 gtcaaactat attagataaa aaacctgaaa gagttgcaac tattgcttgg ggaaatcatg
      301 atgtagcatt agctttagga atagttcctg ttggattttc aaaagcaaat tacggtgtaa
      361 gtgctgataa aggagtttta ccatggacag aagaaaaaat caaagaacta aatggtaaag
      421 ctaacctatt tgacgatttg gatggactta actttgaagc aatatcaaat tctaaaccag
      481 atgttatctt agcaggttat tctggtataa ctaaagaaga ttatgacact ctatcaaaaa
      541 ttgctcctgt agcagcatac aaatctaaac cttggcaaac tttatggaga gatatgatta
      601 aaattgattc aaaagcctta ggtatggaaa aagaaggtga tgagttaatc aaaaatactg
      661 aagctcgtat atccaaagaa ttagaaaaac atccagaaat caaaggaaaa atcaaaggaa
      721 aaaaagtatt atttactatg attaatgctg cagatacatc aaaattctgg atttatacta
      781 gcaaagatcc aagagcaaat tatttaacag atttaggtct agttttccct gaatcattaa
      841 aagaatttga gagtgaagat agttttgcaa aggaaatttc tgcagaagaa gcaaataaga
      901 taaatgatgc tgatgtaatc ataacttatg gtgatgataa aactcttgaa gctttacaaa
      961 aagatcctct tttaggtaaa ataaatgcaa ttaaaaatgg tgccgttgct gtaattccag
     1021 ataatacacc gttagcagcc tcatgcactc caacaccact ttcaataaac tatactattg
     1081 aagaatacct aaatctttta ggaaatgcat gcaaaaatgc gaaataaaaa acaaataaac
     1141 ctaggcataa tttttgtaat ctgcctaggt cttcttatta caatattttt gtcattaaag
     1201 cttggaacaa aagaaattaa tatcagagat tttttagcaa cttttggaat gggcaataca
     1261 aatgatgatt ttattaaatc aattatatat aaaagaatac ctagaactat ttttgcaatt
     1321 ttagcaggtt ctagtcttgc cataagcggt gtattgatgc aatcagttac tagaaaccca
     1381 atagctgatc caggtatact cggtataaac acaggagcaa gtcttagtgt agtaattggt
     1441 ctttcttttt taggaatttc atcaagcata agccatataa gttttgcaat cattggtggc
     1501 ttagtaagtg caatttttgt atacgcgatt gctgtaagcg gaaaagcagg ccttacccct
     1561 ataaaactag ccttatcagg aacttgtgtt agtatggctt taagcagttt tgtaagtttt
     1621 ttaattttac cgaataataa cgtcttagac aaatttagat tttggcaaat aggtagcctt
     1681 ggagcagcta cattatcttc tatatctaca ctactacctt ttataatttt aggtcacttg
     1741 atagctatat ttatttcatc agatttaaac gctttagcta tgggtgatga aatggctgtt
     1801 ggtcttggag ttaatgttaa taggataaga tcacttgcaa taattgcaag tgtgctttta
     1861 tgttcaagta ttactgcaat tggtggacct attggcttcg taggtcttat agttcctcac
     1921 ttttgtggct tatttataag caaagatata cgcacaatga ccatttcttc atcttttata
     1981 ggtgcagagc tcttgcttat atgtgatata atcggccgta tgttaggtaa accaggtgaa
     2041 attgaagtag ggataattac tgcaataatc gggggtccag tacttattta tgtaactatg
     2101 aaaaatagag gggttaataa ctaatgcaaa atttaattat aggtattcaa aaaagaaaaa
     2161 atagaataac actattttcc tcactatttt tattaataat aatcagtcta tcatttttca
     2221 ttttacttat cggagatgaa agttattctt tttcaacttt gattaaagtc ttaaatagtg
     2281 aaactgttcc tggagctagt ttttcgatta tggaaattag attaccaaaa ttattagcag
     2341 gaattatagc tggctggtct tttggattgg caggatttat ctttcaaact atgttaagaa
     2401 atcctcttgc aagtcctgat ataatcggtg tcacaagttc ttcatctatt gcagcggtct
     2461 tttgcatatt ggtattaaaa acaaatagtt taactactgg aattatttca ataacttgtg
     2521 gactaacatc atctttaata ttatttttac tagctaaaaa agatggtttt tcagcagcaa
     2581 gactgataat attaggtatt ggttttcaag ctgtcacaag agcaggcacc tcatttttat
     2641 tgttgaaagt agcaagatat gaattacaag aagttatgag atggctcagt ggctctttat
     2701 cttttacaaa gttagatgac atacctcttg ttctaatagt aagtattatt gctactatat
     2761 tagttttatt ttttaataaa agactagaaa ttattgaact tggtgaagaa atagcaatcg
     2821 gacttggagc aaatcccgag ctttcaaggc ttgttttaat tttttgcgct gtatctttaa
     2881 ctgctttttc tacttcaatt acaggaccaa tagcttgtat atctttttta gctggtccca
     2941 tagccttaaa tattggcaag aaaagaagcc caatattagc tggattggtt ggaattttac
     3001 tagttttgtt atcagacata ttctctcaaa atattttacc agctagatat ccagtaggtg
     3061 ttgtaactgg cttgttaggt tcaccatact taatatactt actaataaaa atgaacagga
     3121 ggaatatata atgaaaggtc tgtggtcaaa taatcttacc tgcggttatg atgaaaaaat
     3181 aatcttggaa aatataaata taaaaatacc tgaagaaaaa atatcagtta ttattgggtc
     3241 aaatggttgt gggaaatcaa cactcattaa aaccttgtct cgacttataa agccattaga
     3301 gggagaagta ttgcttgata ataaatcaat taattcttat aaagaaaaag atttagcaaa
     3361 acacatagct atattacctc aatctccaat aatccctgaa tcaataacag tagctgatct
     3421 tgtaagccgt ggtcgtttcc cctacagaaa gccttttaag agtcttggaa aagatgacct
     3481 tgaaataata aacagatcaa tggttaaggc caatgttgaa gatctagcaa ataacctagt
     3541 tgaagaactt tctgggggtc aaaggcaaag agtatggata gctctagccc tagcccaaga
     3601 tacaagtatc ctacttttag atgagccaac tacttacttg gatatctcat atcaaataga
     3661 actattagac ctcttgactg atctaaacca aaaatataag acaaccattt gcatgatttt
     3721 gcacgatata aatctaacag caagatacgc tgattaccta tttgcaatta aagaaggtaa
     3781 acttgttgca gagggaaagc ctgaagatat actaaatgat aaactagtta aagatatctt
     3841 taatcttgaa gcaaaaatta tacgtgaccc tatttccaat tcgcctctaa tgattcctat
     3901 tggcaagcac catgttagct cttaataatt gaatattgga attcatttaa aagtcgcaaa
     3961 ttttgaactg accctcaaaa gttaggccaa aaaactaact aaaggaggtc agttttttaa
     4021 tggcaaatac agcacagaat ttaagataaa attagtaaaa gaatatcatg aatgaaaaat
     4081 aggatataga gaataacagc aatattaaaa agatcaggac tgataataaa caaaaagaaa
     4141 gtacaacgac tagttcaaaa gctaaaactt caagtaaaaa gttattcaag aaaatctaga
     4201 aaatactcat cctacaaagg acaggtagga aaaatatcag acaacaaaat aaaaagaaat
     4261 atttattacc gaaaaaaagg ggaaatcgag tcaagtacca acaggctcat ctattccaaa
     4321 aagatattta ctcgatggaa aaactcctag aattagtgct acaaatattg acaatggtat
     4381 cttaggttac tacgaagata tagatgataa aaattataga gtttttgaaa attttatatc
     4441 tgtttcattt ttaggagcag tcttttatca taaatataaa gcaagcctag atatgaaaat
     4501 tcattgttta aaattaaaaa ataaagaatt gaataaagaa gtagcattct atttaacttc
     4561 aattattagg caagctttaa aaaatactga atataaagat caaatatcta gcacagtatt
     4621 gccagatatc aaaattaaat taccgataga cagtagaggt actccagatt ggaattacat
     4681 ggaaagatat agagatagat aaagagagag agggagagat agataaaaag tccaccccca
     4741 ttttttatgg agaattcaaa aatgtaaggt taagcaaaga agaatataaa aatctaaaag
     4801 aaaaattaaa ctcacacaca gatataatga taaataaact atccagatac atggaaagca
     4861 gtggtaagac ctatcaaaac cactatgtga caatcttaaa atggtatgaa gaagataaag
     4921 acaaactaag acagaaaggt ttaaataaaa aaatgaatta tgatgtagga gaatctttat
     4981 agataaaaag aggtggaaag gcactattaa agactttaag tctaaaaagg tatcaaagta
     5041 tgccagagat aaaaaaatat aaatatgaca ctttaaaggc gattagaaaa ttaaagaaat
     5101 gctagaagac ttttagatga cagttaccaa gagggtctaa ttttcactgc tacttatgca
     5161 gtgggaattt gaccttttta tataagcaaa ttctatttca aaattggtta ccaagaaaga
     5221 cttcttttca ctgttaagta taagagggta atttatagaa ttttgttaca ttaagataaa
     5281 tattttctga tggtcaaatt ttaaaaagat taataactct cgataaattt tgaaccttaa
     5341 caattgaata gaccaagaca agacgttaat tatttttgga gcgtaataac ctatccgtca
     5401 agatctttcg gctgaataat tcggcaaaaa aagttctgct aagtaaaaag aaagtgagcg
     5461 ataagtaaga gtttgaatat agggagggat accctatccg ctatgaagaa aatgaggata
     5521 atgatacttc taaggttgaa gccggctcat ccggaataga ggcggaggtg agattcctat
     5581 gttgctaatc caaagcactt gtcaatgtca aaaaacttaa ttaaacaaat aattattaga
     5641 tataaaactt tagaggaggt atatattgga caatgactgg aatggattgg ctgatttaat
     5701 agcaaatctg attgcaaaat atgctggtgc tttagattta gataatctac ccgatccaac
     5761 gccagcaaaa aatcaagaga tgaaaaatag ttttgacatg gcaaaaacac aaattgagac
     5821 ggactaataa aagtgttata atatacttga aatgatagtc caaacttaaa ttaaaaaaat
     5881 tatatatgat atagaaaaat tatattgaga ttaatactat gtcaaaagaa aaaataaaag
     5941 tatacctcta tacacgagta tctacgtcaa tacagataga ggggtattcg ttagaggcac
     6001 aaaaatcacg aatgaaagct tttgctattt acaacgatta tgaaattgtt ggagagtatg
     6061 aagatgccgg gaagtctggc aagtctattg aaggtagaat acagttcaat cgaatgatgg
     6121 aagatataaa atctggaaaa gatggagtat cttttgttct tgtgtttaag ttatcaagat
     6181 ttgcaagaaa tgccgctgat gttctttcta cacttcagat aatgcaggat tatggagtta
     6241 atcttatctg cgttgaagat ggcattgatt catctaaaga tgctgggaaa ctaatgattt
     6301 ctgttttatc agctgtggct gaaatagaaa gagaaaacat ccgtattcaa acaatggaag
     6361 gtcgcattca aaaagcaagg gaaggaaaat ggaatggtgg ctttgctccg tatggatata
     6421 aacttgaaga cggcaagctg tttataaatg aggaagaggc agttgcaata agaacgattt
     6481 tcgaccagta tgtaaatact acgataggag ccaatgggat atctaaatac ttagagaatc
     6541 atggaattag aaaaatccca agacagaatg gtaagaatcc tttgtttgat gcaggtctta
     6601 taagaaagat attaaagaat cctgtatata atgggaaaat agcatttgga agaagaactt
     6661 tagaaaaagt tcatggtaca agaaatgaat ataagcaggt tgaacaagat gaatatctaa
     6721 tatctgaagg gatacatgaa gctatagttt ccgatgaagt ttggcaagct gctcaggtta
     6781 agctaaaatc tcaagcaaag aaatatgagc atgtgaataa aggaaaagat acacgcacac
     6841 acttgctttc tggaattgta aaatgcccga tatgtggagt gggaatgttt gggaacaagt
     6901 gtatcaagaa aaagaaagat ggcacaaagt ataaagattt ttattactat ggttgtaacc
     6961 ataggcagat gataagaggt cataagtgta cattcagtaa gcaaattaga gaagaattgt
     7021 tagatgatgc tgttgcagag gtgattgtca agatagtaag taatccgaaa tttgcttcta
     7081 tgatgcaaga aaaaatcaac atgaaggtgg atacctctga aatagaaaaa gaaatagata
     7141 attaccaaaa ggaattgaga aagagtcatt ctacaaagtt taagctaatt gaggaaatag
     7201 ataatttaga tgttgaagat aagcattata agagaaggaa acaggattta gatgatagac
     7261 tatatcgtat gtatgacaaa gtagatgaat tagaatcatc actaattgat gcgaaagcaa
     7321 agaaacagac tattgaagct gaaaagctta caggagataa catatataag gttctgatct
     7381 attttgataa gctctataaa gtcatgaatg atgtagagcg taggcagtta atttcagctt
     7441 tgatttctga aattcaagtt tatgaagaaa aacaatctaa cggacaatgg ctaaaatcca
     7501 ttacttttaa gctaccaatt atagaagaaa atctcaacat aggtttggac aatgatgaac
     7561 aagttgaatg tgtaagttta ttagagaaac gtagttaatc ctcaaaaggt tccccgaacc
     7621 ttttgagtcc cactgacgca tcacgctgag gcggtatcac tgcttgtacg agttgaagca
     7681 tcagcgaagt aaaagtagat gttcagccaa tgcaacgagg tgagaccgaa ggtcgataag
     7741 gtgtgttctg ggtataggac tcgtaaagta agtaggaaat atgatgagat gatacagaga
     7801 gcctgaattg gtcaggtgac gtgcttattt acattagcct gataatgaaa atggagagat
     7861 tatcgatggt tatattggaa ttgtaccaaa atgattattc taaagattta gtagcatttg
     7921 attccataga agatgggaaa gcctttgtag ctcaaatccc gggatacact ctggaaacag
     7981 aagatggttt tgaagtggag tattttaatc ctaaaaacat accggattat atggaaatta
     8041 tttttaacgg aaatattgtt cctttatcta aatttatgtt cgatcctgag gaaaatgtaa
     8101 acattatttg gaaagagatt tcaaatttat ccctcaaaaa tgatagagtg atcgaaggat
     8161 attcaaaaat tgatgcatat gtcgttaaca accatgaagt taaaacctat gtcgaaacaa
     8221 gagaaacgaa ctatcgtaaa gcaaaagact tcttagaaag ccgcggatat gaaatcgaca
     8281 gaagcttttt cggaagtgaa gacggtgaag cgattcttta cagaaaaaaa gggatcgaag
     8341 tttggcattt cttgtgtcac ttagacccaa tgtttgtaga gattgaagat gtagaaggat
     8401 atgttaaaga agaagttgga gagattcaat agtcaaccaa agttccttgt gagtatatac
     8461 ctttgctagt aaaagagtca aaacgttata acgaaggggg ggaagaaaat ccttgagtgt
     8521 aagtcttttt cttgtttaga gggaaagtat tagagtagcc gctcataatt tatactagat
     8581 ttgataactg atttgagaca gattttattt ctataaatat tgtgaaaatt ttttaatacc
     8641 aaagatttaa agttactgtg ttaaaaatcc tctccacttt taaactaatg tattataaaa
     8701 gccagttgac acaagtgtta cacctgtgat agaattaact tgaggtgatg aaaatggcaa
     8761 ccgataaaaa tagaataatg attagcttgg atgataaaaa tctagagaaa cttgaaaatt
     8821 tagtagaaga tgctagagat agaaggggaa tgcgactaac aaagtcccag gttattgaat
     8881 tactcctaaa taccgttgat tatttcgatg atattatggg agctatttac tcaaaaaaat
     8941 aagagcaaaa ttgctattat tttttacaaa tcagtgtaac actagtgata taggagtgat
     9001 gaaatggttg tttatattag acaaagtaaa ttaccaagtg aggtttcaat taataaatac
     9061 aatgctcaag taggtgccta cttacaagga gaagaagtta ttttatatca atctttttct
     9121 gaaataaaag agttaacaag tgaagatata gttgtagatt atataatgga gactagagca
     9181 ttactaaaaa tgatgggctt aaacgttccg gtttatgact atcctattga gcttaaagag
     9241 ttttatggtc gaaagattta tgctggtatt ttaggagaga ttgtgaatat acctgataat
     9301 tggggaaaat ttattaagcc taaagctggt tcaaaagtct ttactggaag agttgttaat
     9361 ggaacccatg atttaatagg tattggtcta cctttcgact atcctatatg gattagtgag
     9421 gttgtagaat tcatagctga atggcgttgt tttgtgttag atggtcgcgt attagatgtt
     9481 cgaccctata caggtgatta tcatgcacaa tttgatgcaa gtgtaattga tgaagcgata
     9541 tcatgttgga aagatgcgcc aatagcttat ggactagata tcggtgttac tcgcgatggc
     9601 agaacacttg ttgttgaagt aaatgatggt tatgcattgg gaaattatgc cctatctcct
     9661 ttaaaatcaa tcaattttca tagagctagg tggaaagaaa tggtaaaacc ctattttgaa
     9721 aaaaatgaaa tttttaaaat ccaacaggat gttgttttct aaaataaata tggtatataa
     9781 ccaatataaa aagcatcaaa atgaacaagg aggaatctga tgcttggagc aattattgga
     9841 gatattgtcg gttctgttta cgaatggaac aatatcaaaa caaaggactt tcctttattt
     9901 cggaaggact gcttttttac agatgacacg gttatgacct gtgctgttgc agaagcaatt
     9961 atgaatggtg gacaaaaaga cgatttcatt gacgcgatga agaaatatgg cagaatgtat
    10021 ccgaatgctg attacggtgc tcggtttaat gcatggctaa acagcgataa ccgtgagcct
    10081 tataatagct ttgggaatgg atcagctatg cgtatttctc catgtgcttg gatcatggac
    10141 tgtggttttt atgcgaaaac tggtatatgg ccatcatcta gaggacttac gagtctttct
    10201 gcagaggtaa ctcataatca tccagaaggt gtcaaaggcg ctatggctac agctgatgct
    10261 atctttctat gtcgttttta ctttggtggt tattgtagag agtacgagca atcaattaac
    10321 gacaatctta cagagtgtaa aagacgaatt aaggattata tagaaaaaga atacgattac
    10381 aatctatctc aaactttaga tgaaatccgt cctaactatc gttttaacga aacatgtcag
    10441 gaaactgtcc ctcaagccat tatc
//
LOCUS       DAVBHS010000067        10463 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVBHS010000067 DAVBHS010000000
VERSION     DAVBHS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10463
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.067"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(20..616)
                     /locus_tag="VQ651_001209"
                     /pseudo
     CDS             complement(20..616)
                     /locus_tag="VQ651_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(1204..1980)
                     /locus_tag="VQ651_001210"
     CDS             complement(1204..1980)
                     /locus_tag="VQ651_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HET2754213.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            2102..2848
                     /locus_tag="VQ651_001211"
     CDS             2102..2848
                     /locus_tag="VQ651_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2754214.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            2861..3841
                     /locus_tag="VQ651_001212"
     CDS             2861..3841
                     /locus_tag="VQ651_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HET2754215.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            4036..4356
                     /locus_tag="VQ651_001213"
     CDS             4036..4356
                     /locus_tag="VQ651_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2754216.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            4402..4710
                     /locus_tag="VQ651_001214"
     CDS             4402..4710
                     /locus_tag="VQ651_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2754217.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            4727..6025
                     /locus_tag="VQ651_001215"
     CDS             4727..6025
                     /locus_tag="VQ651_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2754218.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQTLGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            6176..8614
                     /locus_tag="VQ651_001216"
     CDS             6176..8614
                     /locus_tag="VQ651_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HET2754219.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            8633..9301
                     /locus_tag="VQ651_001217"
     CDS             8633..9301
                     /locus_tag="VQ651_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HET2754220.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEVGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            9319..10407
                     /locus_tag="VQ651_001218"
     CDS             9319..10407
                     /locus_tag="VQ651_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HET2754221.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
BASE COUNT         3430 a         1482 c         2079 g         3472 t
ORIGIN      
        1 tcttatttca ttttactata ccatcaataa gtgtgaaaag atcttttaca taagaccaag
       61 ccttatcatc tttgtaacca ccttgagctt gtagcatatt tgttaattga gcaaaggcgc
      121 tagaagagtt tgctgggtca gcagttgcga tttttccttt tagttcaggt ttgagaaggt
      181 cgctatatcc ttcgatgttc atgcctttag ttaaatcagg gtggacgatt aaaacactac
      241 catctagtgt ataaggagta gagaagccag ttgtgttttg atattctttg ataacattat
      301 cattttcttt tgaaatatag ttttcaaaga gttctccgtg ggtagcatat tgtgtataag
      361 aaccaccaaa gataatatca gctacaggaa cttctttttc tgactctagt tttttgaaaa
      421 gttctccagt accagcttga atcagttcta ttttgatacc atatttttct tcaaaggcag
      481 gaatagttgc tccaattaag ccttctgagt ttggtgaata aacgactagc gaaccaccgt
      541 ctcctttatc agatgaactg tcatcggcag attcattaga agaacaagca gcataataca
      601 tccatttctt tttcatgatg gatacctccg ttgtgttatt taagtttatt ttaaaacaat
      661 gtaagcgttt ttaaaaccta caattctatt ctatagtgta ttgaatctat aacagtacac
      721 attgactgct aaaatatttc tataaattaa tttgactttc ctgatagaga tgttcacatc
      781 ttatttcaat tcactatatt agagtaaaat tctctacaaa aaaaaagaat agcctatttt
      841 actatttttc cgagtgattt caattccttt ggggaaatat ggagatactt tttaaatcct
      901 gacaaatggt tgtttctttt tctaaatcgg tgatactgta tcggagaatg cgcgtgaggt
      961 cacaaaggct gcgatagagc ttctatggag aatttctttt tggagagatt ttttaaagga
     1021 atgagacatc cgctacctcc ttggaaggtt tttttgtgat aagtatagca gtttaaaaat
     1081 tctagagcaa ggtaaaatag aaaaggtttg gctaaaattt tttagctaaa ccttttgaaa
     1141 agttaagtat gtattataga tgcttgttat ctgcaaatag ctagagcatt tcaaggaaat
     1201 aaactagaaa taacaattaa tatggtggtt cagaaatacc ttttgataat caataagatc
     1261 ttcaggatga agtgcgttga ttccatccat ttcatatttc cgatttaata aacgatattt
     1321 gttttcacca aattgatgaa aagggagtag ttgaacttgg tcgatattta atgagttaaa
     1381 tagagtagcg aatttttctg catcctctaa actattgtta aaattaggaa taactgggat
     1441 tcttaaaacg atagttttat tttgtgaaaa agcataatga atgtttttaa taatcatttg
     1501 attaaaaacc ccagtcactt ttttatgttt tatagaatta taatgtttta ggtctgtgta
     1561 gataaaatcc acatattgaa ttaaatcaat aaatttttca tgatcaacaa aggcagtagt
     1621 ttcaatggca gtgtgtatgt gatgttcttt agctgatttt aagatagctt tagcaaattc
     1681 aaactgagca aatatttcac ctcctgataa agttaaacct ccaccggatt cttcgtaaaa
     1741 ttctttgtct tttaatacct ctgtaataat ttcttctaca gtcttttctt caccgactaa
     1801 ggtgaatttc tctcgttgag catctttcat tttttcaggt ttcattcttt gagattcagg
     1861 attagaacac catggacagc gcagaggaca tccttttaaa aaaacagttg tacgaatacc
     1921 cgggccgtca tgaattgaaa agtgttgaat attaaaaata attcctttag atatttccat
     1981 aagagttcct cctcttcata agtctatcct atcacaaaaa cgaaagaaaa acaattacga
     2041 acgaaaggtt aatttgttgt tttttgcttt ttattttggt agaataaaac ggaggtgtaa
     2101 aatgaaaaga ttggaacaaa ttattaaatt agtatcagaa tatgaaaaga tcgacgttaa
     2161 tacattatcg gaaaaattaa atgtatcgaa agtaacgatt agaaaagatt tagataaatt
     2221 agagtcaaaa ggtttattac acagagagca tggatatgct gtattaaata gtggagatga
     2281 cttaaatgta cgtttgtcaa ttaattatga aattaagaga aaaattgttc aggaagcagt
     2341 aaaattggtg tcagataatg aaacgataat gatagaatct ggatcgacct gtgctttact
     2401 tgctgaggaa atttgcaagc aaaaaagaaa tgttacgatt gtaacaaatt cattttttat
     2461 agcaaatttt gtgagagctt atgattcatg tcgtattatt gttcttggtg gagagtttca
     2521 gaaggattca caggttactg tgggaccttt attaaaagaa atgatacaga cttttcatgt
     2581 gcatcaagct tttgttggga cagatggata cgataaagag atgggcttta caggaaaaga
     2641 tttaatgcgc agtgaggtag ttcaatatat ttcagcagcg tcggataaag tcattgtcct
     2701 aactgactca agtaaatttg ataaaagggg tacagtaaga agatttgctt taagtcaagt
     2761 ctatgaagta ataacagacg aaaaactttc taaacaaaat atagctacat tagaaaatgc
     2821 tgggataatg gttaaggtag tttcgtaaga ggttaagtgt atgaatcaag ataggaataa
     2881 actgctctct aaaattgctt atctgtatta tattgaaaac ttaaatcagt cacaaatagc
     2941 agcaaaatta ggaatttata gaacctctat tagtagaatg ttaacagaag caaggaatgc
     3001 aggaattgtt aaaattgaaa tagagaattt tgataccaat atgtttaagt tggaaaatta
     3061 tgtaaaagaa aaatatggtc tggaaagttt agaaattatt ccaaatgaat ttgatgataa
     3121 tccaacaatt ttatctgaaa gaatttctca agttgcagca ggtgtcctta ggaatctaat
     3181 tgatgataat atgaaaattg gcttttcttg ggggaaaagt ttaagtaatt tagtagattt
     3241 aattcacagt aaaagtgtcc gaaatgttca cttctatcct ctagcaggtg gtcctagtca
     3301 catacacgct aaataccatg tgaatacact gatttatgaa atgtctagaa aatttcatgg
     3361 agagtgtaca tttatgaatg caacgattgt gcaagaaaat aaattgttag cagatggtat
     3421 tttgcaatca agatattttg aaaatttgaa aaatagttgg aaagatttag atatagctgt
     3481 agtcggaatt ggtgatttta gcaataaagg aaagcatcaa tggttagaca tgcttacaga
     3541 ggatgacttt aaagaattga ccaaagttaa aactgtagga gaaatttgtt gtcgattttt
     3601 tgattcaaaa ggtaaagaag tctatgaaaa cttacaggaa agaacgatag caatctcttt
     3661 ggaagattta aaaaatattc ctcaaagttt agctgttgct tacggtgata cgaaagtatc
     3721 ttcgattctt tctgtcttgc gtgctaattt agtaaatcat ttgattacag acaaaaatac
     3781 aattttaaaa gttttggaag aagatgggga tttgactttt agagagattc taggtgagtg
     3841 aaaatgatag actgattcag tttatcgttt ttctttttag ttgattgcac atttgtgctt
     3901 atataaacaa aaatagttta tctgttgttt ttggattgac aactttatta tgtagttgta
     3961 ttctatagtt acaaaagaaa attttaaaat ttcaaatgaa aaaagctttt tacatagtga
     4021 aatgaggagg aatttatgga aattattgtt ccagatcaaa ttatcatggg tttaatttta
     4081 tatgctggtg atgcgaaaca acatatttat aaagcgttag attacataaa aaatggtaca
     4141 tgtgaacggt gtgaagaaga aatccagtta gctgatgccg ccttattaga agcccataat
     4201 ctacaaacaa aattcttggc acaagaagcg tctggtacaa agacagaaat tacagctctc
     4261 tttgttcatt cacaagatca tctcatgact agtatgacgg agattaattt aatcaaagaa
     4321 attattagtt tgagaaaaga acttcataaa aaataatact agagtattat cattgttatt
     4381 aacatagagg aggaaaacat aatggtgaag attggtttgt tttgtgcagc aggtttttct
     4441 actggtatgc ttgtaaataa tatgaaaatt gcagcgcaat ctagtggagt tgaggcagaa
     4501 atagaggcgt tttctcagtc taaattagcg gattatgcgc caaatataga tgttgcacta
     4561 ttgggtccac aagttgctta tacattagat aaatcaaaag aaatttgtga taagtgtgat
     4621 gttccgatag ctgttattcc gatgatggac tatggtatgt tagatgggaa aaaagtatta
     4681 gatttggccc tatctttgat tagtgggtaa gaaaaggaga tttattatgt caaagatgga
     4741 tgttcagaaa atcattgcac cgatgatgaa gtttgtgaat atgcgtggca ttatagctct
     4801 aaaagatggg atgttagcaa ttttgccatt gacagtagtt ggtagtttgt tcttgattat
     4861 gggacaattg ccgttcgaag gattaaataa gagcattgct agtgtttttg gagctaattg
     4921 gacagagccg tttatgcaag tatattcagg aacttttgct attatgggtc taatttcttg
     4981 tttttcaatt gcctattctt atgctaagaa tagcggagta gaggctttac cagctggagt
     5041 tctatctgta tctgcattct ttattttgct aagatcatct tatatcccta aacaaggtga
     5101 ggcgattggg gacgctatta gtaaagtttg gtttggaggc caaggaatta tcggtgctat
     5161 cattataggt ttggtagtag gaagtattta taccttcttt ataaagagaa aaattgttat
     5221 taagatgcca gaacaagttc cacaagctat tgccaaacag tttgaagcaa tgattccagc
     5281 atttgtaatt ttcttatctt ctatgattgt atatatttta gcgaagtcat tgactaatgg
     5341 cggaacattc atagaaatga tttattctgc tattcaagtt ccgttgcaag gtttaactgg
     5401 atctttgtat ggtgctattg gaattgcatt ctttatatca tttttgtggt ggtttggtgt
     5461 tcatgggcaa tcggtagtaa atggagtagt gacagctctg cttttatcta atcttgacgc
     5521 taataaagct atgttagcct ctgctaatct atcattagaa aatggtgcac atattgttac
     5581 tcaacaattt ttagattcat ttttaattct atcaggttca gggattacgt ttggtcttgt
     5641 agttgccatg ctttttgcag caaaatcaaa acaataccaa accttaggaa aagttgcagc
     5701 ttttccagca atatttaacg taaatgagcc agttgtattt ggatttccga ttgtcatgaa
     5761 tccagttatg tttgtacctt tcattcttgt tcctgtactt gcagctgtga tagtatatgg
     5821 agctattgca acaggtttca tgcagccatt ctcaggggta acattgcctt ggagtacacc
     5881 agctatttta tcaggatttt tggtgggtgg atggcaagga gttattactc agctggtgat
     5941 attagcgatg tctacattgg tttattttcc attctttaaa gtacaggatc gtttagctta
     6001 ccaaaatgaa atcaaacaat cttagaggta tttgtgtgtt actgttaaac tcacacattt
     6061 gtgctaaaaa ttagagagtt aaaatttttc tagttaaaag cttgaaaatt tctataaaaa
     6121 tcggtattat attttcgaaa gaaataaaaa tattttcgaa agaaaggtgg ttacgatggt
     6181 aaatacagaa gtagcaagaa caacaatcaa gacagaatat tttggcagcc ttactgaaag
     6241 gatgaacaaa tatcgagaag atgttttaaa taaaaaacct tatattgatg ctgagagagc
     6301 agttctagca acacgcgcct atgaacgata caaggaacaa cctaatgtcc taaaacgtgc
     6361 atatatgctg aaagaaattt tggaaaatat gactatctat attgaagaag aatctatgat
     6421 tgcgggaaat caagcttctt ccaataaaga tgctcctatt tttccggaat atacgctaga
     6481 atttgttctc aatgagttgg atctttttga aaagcgtgat ggagatgttt tctatattac
     6541 agaagaaaca aaagaacaac ttagaagtat tgctccgttt tgggaaaata ataatttacg
     6601 tgctagagct ggtgccttat tacctgaaga agtgtctgtt tatatggaaa caggattctt
     6661 cggtatggaa ggtaagatga attctggaga tgctcaccta gcagttaact atcagaaact
     6721 tttgcaattt ggtttaagag gttttgaaga gcgggctcgt aaagcaaaag tagctctaga
     6781 tttaacagat ccagcaagta ttgataaata tcatttttac gactctatat ttatcgtaat
     6841 cgatgctatt aaagtatatg caaagcgctt tgttgctctt gctaaaagtt tagccgaaaa
     6901 tgcaaatcct aaacgtaaga aagaattact tgagattgca gatatttgct ctagagtccc
     6961 atatgaaccg gcaactactt ttgcagaagc tattcaatca gtttggttta ttcaatgtat
     7021 tttacaaatt gaatctaatg gccactctct ttcatatggc cgttttgatc aatatatgta
     7081 tccatatatg aaggctgatt tagaaagtgg taaagaaaca gaagatagca ttgttgaacg
     7141 tctgacaaat ctttggatta agacaattac aatcaataag gttcgcagtc aatcacatac
     7201 attttcttca gcaggaagtc ctttatatca aaatgttaca attggtggac agactcgaga
     7261 taagaaggat gctgttaacc cattatctta tttggtatta aaatcagttg cacaaaccca
     7321 tctaccgcaa cctaatctaa ctgtacgtta ccatgcaggt ttagatgctc gtttcatgaa
     7381 tgagtgtatt gaagtgatga aacttggttt tggtatgcct gcatttaata atgatgagat
     7441 tattattcct tcttttattg caaaaggagt attggaagat gatgcttatg attacagtgc
     7501 cattggatgt gttgaaacgg cagttccagg gaaatggggc tatcgttgca caggtatgag
     7561 ttatatgaac ttccctaagg ttctacttat cacgatgaat gatggaattg atccggcttc
     7621 gggtaaacgg tttgcaccaa gctttggtca ttttaaggat atgaagaact tttctgaatt
     7681 agaaaatgct tgggataaaa cactaagata tttgacacga atgagtgtta ttgttgaaaa
     7741 ttctattgat ttatcattgg aacgagaagt tcctgatatt ctatgttcag cattgactga
     7801 tgattgtatt ggtcgtggaa aacaccttaa agaaggtgga gcagtatatg attatatatc
     7861 aggattgcaa gttggaattg caaatttgtc ggattcatta gctgcaatta aaaaattggt
     7921 gtttgaggaa gaacgtataa gcccaagtca gctttggcat gcactggaaa cagattatgc
     7981 cggagaagaa ggtaaggtca ttcaagaaat gttgattcat gatgcaccta agtatggtaa
     8041 tgatgatgat tatgctgaca aattggttac tgctgcttat gacatttatg ttgatgaaat
     8101 tgctaaatat cctaatacac gttatggaag agggcctatt ggaggaattc gttattcagg
     8161 aacatcttct atctcagcca acgtagggca gggacgtgga acattagcaa ctccagatgg
     8221 acgcaacgcg ggtacaccgt tagcagaggg ttgttcacca tcacataata tggatcaaca
     8281 cggccctaca tctgttttaa aatctgtttc aaaattacca acagatgaaa tcgtaggtgg
     8341 ggttctctta aatcagaaag taaatcctca aacgttagcc aaagaagaag ataaattaaa
     8401 actaattgct ttgttacgaa cattctttaa tcgtttacat gggtaccata ttcaatacaa
     8461 tgttgtttcc agagagacgt tgattgacgc tcagaaacat cctgaaaaac acagagactt
     8521 aattgttcgt gttgcaggat actctgcatt cttcaatgtt ctttctaagg caacccaaga
     8581 tgacattata ggacgtactg agcatacttt gtaaaataaa gaggttcttt ttatggaatt
     8641 tatgcttgac acattaaatt tagatgagat taaaaagtgg tctgaaattt tgccgctagc
     8701 tggggtaact tcaaatccca ctattgcaaa aagagagggt tctattaatt tttttgaacg
     8761 aatcaaagat gtaagagaat tgattggctc tacaccctct attcatgttc aggtgatttc
     8821 tcaagatttt gaaggcatct taaaggatgc tcatgaaatt cgaagacaag caggagatga
     8881 tatatttatc aaagtacctg ttactccagc tggattacgt gcaataaagg tgctaaaaaa
     8941 agagggctac catatcactg caacagctat ttatacagtt attcagggat tattagctat
     9001 cgaagtagga gcggattacc tagctccata ttataataga atggaaaatc tgaacattga
     9061 ttcaaattct gtcattcgtc aattagctct tgctattgat agacagaact ctcctagtaa
     9121 gattttagct gcatccttta aaaatgtagc acaagtaaat aatgctttag ctgcaggtgc
     9181 gcatgctgtt acagcaggag cagatgtttt tgaatcagct ttcgccatgc catctatcca
     9241 aaaggcggtt gatgattttt ctgacgattg gtttgttact caaaatagtc gttccattta
     9301 gatagagagg aaatacatat gagaattttt gctagtcctt ctagatatat tcagggggaa
     9361 aatgccttgt ttgaaaatgc caaatcaatt ttggatttgg gaaattaccc tattctatta
     9421 tgcgatcagt tggtttatga tattgttgga aaacgatttg aagattatct acataggtat
     9481 ggtttccata ttgttctggc gctatttaat ggtgaagctt ctgacaatga aatcaatcga
     9541 gttgttgcct tggctgagaa agaaaattgt gatagtatta tcggtcttgg tgggggaaag
     9601 acgattgata gcgcaaaagc tattgcagat ttgattgaaa agcctgttat tattgctcca
     9661 acaattgcat cgaccgacgc acctgtatct gctttatctg ttatttatac agatgaaggt
     9721 gcatttgatc attatctatt ttattctaaa aatccagatt tagttttggt tgatacaaaa
     9781 gttatttcac aagcccctaa gcgtttatta gcgtctggta ttgcagatgg tttagcaact
     9841 tgggttgagg cgcgtgcggt tatgcaggca aatggaaaaa ctatgttggg acaacagcaa
     9901 acattggctg gagttgcaat tgcgaagaaa tgtgaagaaa cgctgtttgc agatggttta
     9961 caggctatgg cagcttgtga agctaaagtg gtgacaccag cattagaaaa tattgttgaa
    10021 gctaatactt tattgagtgg cctaggtttt gaaagtggag gattagctgc ggcgcatgca
    10081 attcataatg gttttactgc attgacaggt gacattcatc atttaacaca tggtgaaaaa
    10141 gtagcttatg gaactttagt acaactatta ttggaaaata gacctaaaga agaacttgat
    10201 aagtatattg agttttacaa aaaaattggt atgccaacaa ctctaaaaga aatgcatttg
    10261 gatcaagttg gatatgatga tttaataaaa gttggtaaac aagcaactat ggagggtgag
    10321 acaattcatc agatgccgtt taagatttcg ccttcagatg ttgctcaagc tattatcgct
    10381 gtagatgcct atgtaaattc aaaataaaca ataaggacta ctgttttcca aatggtagtc
    10441 ttttattgat cctcgtattg aat
//
LOCUS       DAVBHS010000068        10319 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVBHS010000068 DAVBHS010000000
VERSION     DAVBHS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10319
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.068"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            86..1261
                     /gene="mutY"
                     /locus_tag="VQ651_001219"
     CDS             86..1261
                     /gene="mutY"
                     /locus_tag="VQ651_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET2754222.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     LIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1316..2020
                     /locus_tag="VQ651_001220"
     CDS             1316..2020
                     /locus_tag="VQ651_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2754223.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2013..3362
                     /gene="vicK"
                     /locus_tag="VQ651_001221"
     CDS             2013..3362
                     /gene="vicK"
                     /locus_tag="VQ651_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HET2754224.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3364..4173
                     /locus_tag="VQ651_001222"
     CDS             3364..4173
                     /locus_tag="VQ651_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET2754225.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4264..4506
                     /locus_tag="VQ651_001223"
     CDS             4264..4506
                     /locus_tag="VQ651_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HET2754226.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4508..4762
                     /locus_tag="VQ651_001224"
     CDS             4508..4762
                     /locus_tag="VQ651_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET2754227.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4809..5582
                     /locus_tag="VQ651_001225"
     CDS             4809..5582
                     /locus_tag="VQ651_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HET2754228.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFTTKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLAKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPASIFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSSQRILLEKQSKKIEELHKMIQDLIEKT"
     gene            complement(5842..6828)
                     /locus_tag="VQ651_001226"
     CDS             complement(5842..6828)
                     /locus_tag="VQ651_001226"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HET2754229.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGLYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            7022..9490
                     /gene="gyrA"
                     /locus_tag="VQ651_001227"
     CDS             7022..9490
                     /gene="gyrA"
                     /locus_tag="VQ651_001227"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET2754230.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIALRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEE"
     gene            9490..10233
                     /gene="srtA"
                     /locus_tag="VQ651_001228"
     CDS             9490..10233
                     /gene="srtA"
                     /locus_tag="VQ651_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HET2754231.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         3205 a         1752 c         2303 g         3059 t
ORIGIN      
        1 agatagaaaa gttatatact atctattgaa aatattataa atcagataca aagacaggtg
       61 aaaccttctg tgttatacta gagatatgtt agatttgaaa gaatacggta tcgtcatgtg
      121 gccggaggag aaagtcattt ctttccgtga gaaacttctc gcttggtatg atgaaaacaa
      181 aagagatttg ccttggagga gaagtaaaaa tccttatcac atctgggtat ctgaaatcat
      241 gcttcagcag accagggtgg atacagttat cccttactac gaaagattcc tggactggtt
      301 tccaactgtc gaaagtctgg caactgcgcc tgaggagagt ttactgaaag cttgggaggg
      361 cttgggctat tattctcgag ttcgcaatat gcaggctgca gcccagcaga ttatgactga
      421 ctttggtggc caatttccaa atacctatga aggaatttcc agcttgaaag ggattggacc
      481 ttacacagca ggagccattt ccagtattgc ttttaacttg cctgagccag ctgtagatgg
      541 taatgtcatg cgggtcttgg cgcgtctgtt tgaagtcaac cacgatattg ggattccaag
      601 taatcgaaaa atttttcagg caatgatgga gatcttgatt aatccggatc gtccgggtga
      661 ttttaatcaa gccttgatgg acttagggtc tgatattgag tctcctgtaa atcccagacc
      721 tgaagaaagc ccagtcaagg actttagtgc ggcatatcag aatggaacca tggaccgtta
      781 tccaattaaa tctcctaaga aaaagccggt tcctatttat cttaaagcat tagttgtgaa
      841 aaattctcaa ggacaatttt tacttgaaaa aaatgaaagc gaaaagctct tggcaggttt
      901 ttggcatttc ccccttatag aagttgataa cttttcgcaa gaagagcagt ttgacctctt
      961 tcatcaggtt gcggaagaaa gtgtgaactt tggccccagt ccagaagaga gtttccagca
     1021 ggactatgac ctagatgttg attggcttga tgtttgtttt gatacagtcc agcatgtttt
     1081 tagtcatcga aaatggcatg tacagattgt agcaggtcag gtgagtgact tccatgattt
     1141 ttcagatagg gaagttcgtt ggctttcacc agaagaattc aagaattacc cacttgccaa
     1201 accccaacaa aaaatctggc aggcttatgc acaagccaac ttagacagta gccaagacta
     1261 gctattgtgt cttctgacta ttttttggta taatagtaga gaaaaaggtg aacatatgaa
     1321 aaaaatacta attgtagatg atgagaaacc aatctcggat attatcaagt ttaatatgac
     1381 caaggaaggt tacgaagttg taactgcttt taatggtcgt gaagcgctag agcaatttga
     1441 agcagagcaa ccagatatta ttattctgga tttgatgctt ccagaaattg atggtttaga
     1501 agttgctaag accattcgta agacaagcag tgtgcccatt cttatgcttt cagccaaaga
     1561 tagtgaattt gataaggtta tcggtttgga acttggggca gatgactatg taacgaaacc
     1621 cttctccaat cgtgagttgc aggcgcgtgt taaagctctt ctgcgtcgtt ctcaacctat
     1681 gccagtagat ggtcaggaag cagatagtaa acctcaacct atccaaattg gggatttaga
     1741 aattgttcca gacgcctacg tggctaaaaa atatggcgaa gaactagact taacccatcg
     1801 tgaatttgag cttttgtatc atttagcatc gcatacaggt caagtcatca cgcgcgaaca
     1861 cttgcttgag actgtctggg gttatgacta ttttggtgat gtccgtacag ttgatgtgac
     1921 tgtacgacgt ctgcgtgaga agattgaaga tacgcccagc cgaccagagt atatcttgac
     1981 gcgccgtggt gtagggtatt acatgagaaa taatgcttga tttactgaaa caaaccattt
     2041 ttaccagaga ttttatcttt atcctgattt tgttaggttt catccttgtt gtgaccctct
     2101 tattactgga aaatagacgt gataatattc agttgaagca aatcaatcaa aaggttaaag
     2161 atttgattgc aggagattat tccaaggttc ttgatatgca aggtgggtct gaaatcacca
     2221 atattaccaa taatttgaat gacttgtcgg aggttattcg tctcactcag gaaaatctag
     2281 aacaagagag taagaggcta aatagtattc tgttttatat gacagatggg gttcttgcga
     2341 ccaaccgtcg gggtcagatt atcatgatta acgatacagc caagaagcaa ctggggttgg
     2401 ttaaggaaga tgttctgaat agaagcattt tggaattgct caagatagaa gaaaactatg
     2461 aattgcgtga tttgattacc caaagtccag aattgttgct agattcccaa gatatcaatg
     2521 gcgaatattt gaaccttcga gttcgctttg ccttgatacg tcgagagtct ggctttattt
     2581 caggtttggt ggctgttttg catgatacga cggagcagga gaaggaagaa cgcgaacgaa
     2641 gactctttgt ttccaatgtt agccatgagt tacggactcc tctgactagc gtaaaatcct
     2701 atcttgaagc cttggatgag ggggctttgt gtgaaactgt agcaccagac tttatcaagg
     2761 tttctcttga tgagaccaac cgtatgatgc gcatggtgac ggatctcctc catctttcac
     2821 gtattgataa tgctaccagt cacctagatg tggaactgat taacttcact gcttttatta
     2881 cctttatcct caatcgtttt gacaagatga aaggacagga aaaggagaaa aaatatgagt
     2941 tggtgagaga ttatcccatc aattctatct ggatggaaat tgatacagat aagatgacgc
     3001 aggttgtcga caatatttta aataatgcta ttaagtattc gccagatggg ggtaaaatca
     3061 ctgtcagaat gaagacaact gaagaccaga tgattttatc catttctgac cacggtttgg
     3121 ggattcctaa gcaggattta ccacgtatct ttgaccgttt ctatcgtgtg gatcgtgcta
     3181 gaagtcgtgc acaaggtggt acaggtctag gactgtctat cgctaaagaa attatcaaac
     3241 aacataaggg ctttatttgg gccaagagtg aatacggcaa gggttcaacc tttaccattg
     3301 tgctccctta tgataaggat gcagtgaaag aagaagtatg ggaggatgaa gtagaagact
     3361 agaatgagtg aaataggctt taaatacagt attttagcgt cgggttccag tggaaattct
     3421 ttttatctgg aaacctcaaa aaagaagctt ttagtagatg caggcttgtc tggcaagaaa
     3481 attaccagtc tgctagctga aattaaccgt aagccagaag acctggatgc catcttgatt
     3541 acccatgagc attcagatca tatccatgga gtaggcgttt tggctcgcaa gtatggtatg
     3601 gatctttatg ccaatgaaaa gacctggcaa gctatggaaa atagtaaata tcttggcaag
     3661 gtggattctt cgcaaaagca tatttttgaa atgggtaaaa ccaaaacctt tggagatatc
     3721 gacatcgaga gttttggtgt aagccatgat gcagtcgcac cgcagttcta tcgctttatg
     3781 aaggatgata agagttttgt cctcttgaca gatacaggtt atgtcagtga ccgtatggcg
     3841 ggcattgtcg aaaatgcgga tggttatctt atcgaggcca accatgatgt agagattttg
     3901 cgatcaggtt cttacgcttg gcgcctcaaa caacgaatcc tatctgacct tggtcacctt
     3961 tctaacgagg acggtgctga agctatgatt cggacgctag gaaatcgtac taagaagatt
     4021 taccttgggc atttatctaa ggaaaacaat atcaaggaac tggctcatat gaccatggtc
     4081 aatcagctgg ctcaagctga tctgggagtc ggagtagact ttaaggttta tgatacctca
     4141 ccagataccg caacaccatt gacagagata tagaaagaac gctgagaagg tgttcttttt
     4201 atattgactg gacacctaaa aagtaataca atggtgttac cattaaaaaa gggagcacaa
     4261 aagatgacta ctattacatt aaaagtttct gaagctgata aaacatttat gaaagcaatg
     4321 gctaagtttg aaggagtttc cctttcggaa cttattcgca ccaaaactct tgaagcccta
     4381 gaagatgaat acgatgctcg tgtggcagat ttagcctatc aagagtattt agaagacttg
     4441 gaaaaaggag ttgaacccat tacttgggaa gaaatgatgc atgatttagg cttgaaggat
     4501 gaataatttg tataaattag ttccaacaag acgttttatc aagcaattga aaaaattgga
     4561 ccgttatacg cagaagctaa ttacaaacta tttacaaacc aatgttttgg aagatccaag
     4621 acgacacgga aaggctttgg ttggtaatcg cgttggtcaa tggcgctata gaattggtaa
     4681 ttatcgagtt atcgtacaaa ttgtagatga tgaattagtt gttgctactc tagaagttgg
     4741 tcatcggaga gatatttatt aaattaaagc tgagtaataa cagtatagaa agtaaggaga
     4801 gtaaaaattt gaagaaaaaa tggttttttg cagattatta tgacacaaca attattttac
     4861 ttgctttaat ctctgttatt ctggtattac ttggatttgc tgagatgatt gatttggaca
     4921 atccgcctta tagtattatt gatttagtaa tctggggtgt ttttgtaatt gattatagtt
     4981 ggcgcttttt tacgactaaa agaaaatggc gctttattct tgaaaatatt tttgatttat
     5041 tggctattct ccctttaaat gctattttta cagtgtttcg attagggcgt atctttcgtt
     5101 tagctaaact aacaaaatta ctgaaactga ctcgtttact tagaataatt ggacttacag
     5161 gtaaattaga aagaaaaatt agtagattct tacggacaaa tggcctgatt tatatcttgt
     5221 atgttaatat ctttattgtt ctagtaggga gttcgatttt atccgttgtt gaagaaaaat
     5281 ccttctcaga tagtctttgg tgggctcttg tgacagtaac tactgttgga tatggggata
     5341 ttgttcctgc ttccattttt gggaaatggc tagcagtttt actgatgctt gtcggtattg
     5401 gaacaatagg gatgttaaca agtgctttga cgaacttttt tgtaaaggat aatccagatg
     5461 aacagataaa acttgataaa ctcaaagatg aattatcgag ccaaagaata ttactagaga
     5521 aacaatctaa aaagatagaa gaactacata aaatgataca agatttgatt gaaaaaacat
     5581 aataaaaata aagatgagac aaagtttgag ctgaggagaa atcaagtaag ttttgacaca
     5641 atagaacgca tattatcagg tataaaaact tgatactatg cgttttatag agaaataaaa
     5701 taaaatctga acaaatcaat taggaattta aagcatttta taacaacgtt ttagagtgat
     5761 ggggtgctat ttcaacttca acctactata atacagaaaa aaacaactcc ctgatgattc
     5821 aaggagttgt ctatagttaa attagttttt agaagcttct tggaattctg gatttttcca
     5881 tgcttcgtca ataatagctt gcaattcttt agcagatgct tgcatttttt gagtttctgc
     5941 atcgttcaat ggaatgttta ctggacgaac gataccatgt gcaccaacaa cagctggttg
     6001 accgataaag acattctcaa ctccgtatag accttcttgg aatactgaaa gtggaagtac
     6061 tgcgttttca tcgtcaagga ttgctttagt gatacgagca agggctactg cgataccgta
     6121 gtatgttgca ccttttttgt tgatgattgt gtaggctgca tcacgaacac cttcgaacaa
     6181 ttcaatcaat tcagcttctt gaacattttg agtgtcttta aggaattctt caaggtttac
     6241 accagcgatg ttagcgtgtg accaaacagc gaactcagag tcaccgtgtt cacccatgat
     6301 gtaggcgtgc actgaacgag catccacatc caatttttca gcaagtgctt gacggaaacg
     6361 agctgagtca agtgaagtac ctgaaccgat aacgcgttct ttagggaaac cagagaattt
     6421 ccaagttgag taagtcaaaa cgtcaactgg gttagcagca acaaggaaga tacctttgaa
     6481 accagattca acaacttgag ttacgattga tttgttgata gcaaggtttt tacctacaag
     6541 gtcaagacga gtttcacctg gtttttgagg tgcacctgca gtaatcacaa caaggtcagc
     6601 gtctgcacag tcagagtatt gagctgcata gattttttta ggtgaagtga aggcaagggc
     6661 gtgactaagg tcaagcgcat caccaacagc tttttcatgc aattgtggaa tttcgataat
     6721 tccaagctct tgtgcaattc cttggttaac aagtgcaaaa gcgtaagatg aacctacagc
     6781 accatcaccg acaaggataa cttttttgtg ttgtttagtt gaagtcattg ttttaaacat
     6841 ctccttaatt ttattagggg attttcccta gacaacttca ttctatcact tttaaaaaac
     6901 tttgtcacga atatgcctta tagttctcga tgtaaacgtt ttagtggttt agaggctgaa
     6961 atagatggga atttatggta taatgttgtt acttactaat tgtgaaatga ggcatttatt
     7021 aatgcaggat aaaaatttag tgaatgtcaa tctgacaaag gagatgaagg caagttttat
     7081 cgactacgcc atgagtgtta tcgtagcgcg agctcttcct gatgttcgag atggcttaaa
     7141 acctgttcac cgtcgcattc tctacggaat gaatgaattg ggtgtgaccc cagacaaacc
     7201 ccataaaaaa tctgctcgta ttacagggga tgtcatgggt aaatatcacc cacacgggga
     7261 ttcctctatt tatgaagcca tggtccgtat ggctcaatgg tggagctacc gttacatgct
     7321 tgtagatggt catgggaatt ttggttccat ggatggagat agtgctgccg ctcaacgtta
     7381 taccgaggca cgtatgagca agattgctct ggaaatgctt cgtgatatca acaaaaatac
     7441 agttgatttc gttgataact atgatgccaa tgaacgggaa cccttggtct tgccagcgcg
     7501 ttttccaaac cttttggtta atggagcaac tggtatcgcg gttgggatgg caaccaatat
     7561 tccacctcat aatctgggtg aaaccattga tgcagtgaag ttggtcatgg ataatcctga
     7621 agtgactacc aaggacttga tggaagtctt gcctggacca gattttccaa ctggtgctct
     7681 tgtcatgggg aaatcaggta tccataaggc ttatgaaaca ggtaaaggtt cgattgccct
     7741 acgttctcgt acagagattg aaacgactaa gactggtcgt gagcgcatcg ttgtaacaga
     7801 atttccttac atggtcaata aaaccaaggt gcatgagcat attgttcgct tggttcagga
     7861 aaaacgcatt gagggtatca cagcagtacg tgatgagtca aaccgtgaag gtgttcgatt
     7921 tgttattgaa gtcaagcgcg acgcctcagc caatgttatt ctcaataacc tcttcaaaat
     7981 gacccaaatg caaaccaatt ttggtttcaa tatgctcgct atccaaaatg gtataccgaa
     8041 aattttgtct cttcgtcaga ttttggatgc ttatatcgag caccaaaaag aagtggttgt
     8101 tcgtcgtaca cgttttgata aggaaaaagc ggaagcgcgc gctcatatct tagaaggtct
     8161 cttgattgcg ctagaccata tcgacgaagt gattcgtatc atccgtgcta gtgaaacgga
     8221 tgcggaagct caagctgagt tgatgagcaa gtttaagctt tctgaacgtc aaagtcaagc
     8281 tatccttgat atgcgtcttc gtcgtttgac aggtttggaa cgcgataaga ttcaatctga
     8341 gtatgatgac ctcttggctc tgattgcgga tttagcagat attcttgcta agcctgaacg
     8401 tgtttctcaa attatcaaag acgaattgga tgaagttaaa cgtaaatttt ctgataagcg
     8461 ccgtacagag ttgatgattg gacaggtctt gagtctcgag gatgaggact tgattgaaga
     8521 atcggatgtc ttgattaccc tttctaacag aggctacatt aagcgtttgg atcaggacga
     8581 gttcactgct caaaaacgtg ggggtcgtgg tgtccaagga acgggagtga aagatgatga
     8641 ctttgttcgt gagttagtgt caactagcac ccatgatcat ctgctcttct tcacaaacaa
     8701 gggacgtgtc tatcgtctta aaggttatga aattcctgag tatggtcgga ctgccaaagg
     8761 gctaccagta gtcaatctct tgaaattgga tgaagacgaa agtattcaga cggttatcaa
     8821 tgttgagtct gatcgcagtg atgatgctta tctcttcttt acaacccgtc acggtattgt
     8881 gaagagaacc agtgttaagg agtttgccaa tattcgtcaa aatggtctca aagcgctgaa
     8941 tttaaaggat gaagatgagt taatcaatgt cttgttggca gaaggagata tggatattat
     9001 cattggtacc aagtttggtt atgcagttcg ctttaatcaa tcagccgttc gtggtatgag
     9061 tcgtatcgcc actggtgtga aaggtgttaa ccttcgtgaa ggagacacag ttgttggtgc
     9121 cagcttgatt actgatcaag atgaggttct tattatcaca gaaaaaggat atggtaagcg
     9181 tacagtcgct actgaatacc caacaaaagg tcgtggtggt aagggaatgc agacagctaa
     9241 aattaccgaa aaaaatggct tgctggccgg tcttatgact gttcaagggg atgaggattt
     9301 gatgattatc actgatacag gtgtcatgat tcgaaccaat cttgccaata tttcacaaac
     9361 aggacgtgca actatgggag ttaaagtaat gcgcctggat caagatgctc agatagtgac
     9421 tttcacaacg gttgcggtgg cagaaaaaga agaagttggg acagaaaacg aaacagaagg
     9481 tgaagaataa tgtctcaaaa aaataataaa aagaaaaaca agcgaaaaaa tctgctgaca
     9541 aatatcctag caggatttct gatattactg tcactggctt tgatttttaa tactcaaatt
     9601 cgaaatattt tcatagtctg gaataccaat aagtatcaag ttagccaggt atcaaaagaa
     9661 aaattagaag aaaatcagga tacagaaggc aattttgact ttgattctgt caaagctatc
     9721 tcttcggaag ctgttctaac ttctcaatgg gatgctcaaa aattaccagt tattggggga
     9781 attgcaattc ctgaattgga aatgaatttg ccgattttta aaggacttga taatgttaat
     9841 ctcttctacg gagctggtac aatgaaacgc gagcaagtaa tgggagaagg aaattatagt
     9901 ctagctagtc accatatctt tggtgttgat aatgctaata aaatgttatt ttctccttta
     9961 gataatgcta aaaatggcat gaagatttat ctaaccgata aaaataaagt ttatacttat
    10021 gaaatacgtg aagtcaaacg tgtgacaccg gatcgtgttg atgaagttga tgatagagat
    10081 ggggtcaatg aaatcacatt agtaacctgt gaagaccttg ctgctacaga acgtattatt
    10141 gtcaaaggtg atttgaaaga aacaaaagat tattcacaaa catctgatga aattctaaca
    10201 gctttcaatc aaccatataa acaattttat taatacaaat cagtgaaatc atggatttca
    10261 ctgatttttt aagattttca taaaaatata gtaaaatgaa ataagaatag gacgaatcg
//
LOCUS       DAVBHS010000069        10298 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVBHS010000069 DAVBHS010000000
VERSION     DAVBHS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10298
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.069"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            2..523
                     /locus_tag="VQ651_001229"
     CDS             2..523
                     /locus_tag="VQ651_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2754232.1"
                     /translation="MKFLCSDELTILQILYGASLISFLWMYGQRKQVIKVNMKTRIQW
                     LGIGFVSLLIISLCFSLIHAQESTNQANLIGLQHQIPWFSFLLFLINASMVEEFLYRE
                     ILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYVSLGMFLGLVRYKLDLWGSMGLH
                     LVWNLLVYSLLLF"
     gene            924..1733
                     /locus_tag="VQ651_001230"
     CDS             924..1733
                     /locus_tag="VQ651_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2754233.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNNYSCKALC
                     ECLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIREKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKVSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWNSLLLNENCLVIGASVSGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTNHLSYGNRISPQFPILLITDCLFSHYLESPERQYYYNQTIIHKEE"
     gene            1738..2439
                     /locus_tag="VQ651_001231"
     CDS             1738..2439
                     /locus_tag="VQ651_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2754234.1"
                     /translation="MSQIWTKEKFISQVHGGVIVSCQALPGEPLYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVINEFIKKIKEKYPNQLLMADVSNFDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            2464..3381
                     /locus_tag="VQ651_001232"
     CDS             2464..3381
                     /locus_tag="VQ651_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HET2754235.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQERVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHVACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAAPNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            3409..4950
                     /locus_tag="VQ651_001233"
     CDS             3409..4950
                     /locus_tag="VQ651_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HET2754236.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGNSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGAVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLMANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNAGSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDDRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDIPEIKADSS
                     WEVRH"
     gene            4969..5427
                     /locus_tag="VQ651_001234"
     CDS             4969..5427
                     /locus_tag="VQ651_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2754237.1"
                     /translation="MIISEQSDFRRYASINKHFSKVCDFLENTNLTDLVDGKVDIDGE
                     NVFANCMIYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDEPVKKLVIKVLNEEK"
     gene            5429..7651
                     /locus_tag="VQ651_001235"
     CDS             5429..7651
                     /locus_tag="VQ651_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HET2754238.1"
                     /translation="MKKNIKQYVTLGTAVVLSAFVANSVAAQETETSEVSTPELVQPV
                     APTTPISEVQPKSGNSSEVTVQPRTVETTVKDPSSATEETPVLEKNNVTLTGGGENVT
                     NELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNHLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPRDVGGRDLQISGGATYIDSVIVEKNNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLKKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFKNNKKTEYRNGTHVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSTDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            7691..8800
                     /locus_tag="VQ651_001236"
     CDS             7691..8800
                     /locus_tag="VQ651_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2754239.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEYEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            8957..9847
                     /locus_tag="VQ651_001237"
     CDS             8957..9847
                     /locus_tag="VQ651_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2754240.1"
                     /translation="MKIIGIDIGGTTIKADLYNEFGTSLNQFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYILNHSIDGVGISTAGVVNANTGEIIYTGYTIPGYIGVNFTFEIEKRF
                     GLSTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRAGDKVAKETFEIFVE
                     NLTKGLLTITYLLNPEILILGGGILAKKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            9872..10195
                     /locus_tag="VQ651_001238"
     CDS             9872..10195
                     /locus_tag="VQ651_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754241.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELVSIESNLEMKQQNLIDEVNKTIKENYEKIQYISSSRRGEFVEKIIGRVV
                     EKYGY"
BASE COUNT         3512 a         1486 c         2026 g         3274 t
ORIGIN      
        1 attgaaattt ctttgtagcg atgagttaac tatactacag attctctatg gagcgtcact
       61 catttctttt ttatggatgt atggtcaacg aaaacaagtg atcaaggtta atatgaaaac
      121 taggatacaa tggcttggta ttggattcgt tagcctactg attataagtc tatgttttag
      181 tctaatccat gcgcaagaaa gcacgaatca agcaaactta attggacttc aacatcaaat
      241 tccttggttt tcatttttat tgtttttaat caatgcgagt atggttgaag aatttctgta
      301 tagagaaatt ttatggaact tggttagaaa attagatatt cgagttgctt tgacaagtgt
      361 tttatttgcc ttagcacatc atccaggaac cattatagct tggtgtttgt atgtatcgct
      421 ggggatgttt ttagggcttg tacgctacaa attggactta tggggcagta tggggctaca
      481 tttggtgtgg aacctactag tttatagttt gctgcttttt taaaataagt gatctgattt
      541 atctcataaa tcagtagttt ttataggatt atttttaatt cggaaacaaa aatgtattaa
      601 cagcataaaa taaagaaact aggaacattt tttttatttt ttagaaataa aatgttgtat
      661 agaaattccg aaagaaataa taaaatcaaa ataaaaatta ataaaatagg tatatttgaa
      721 catattgacg tcgacaaatg ttcttgcaag acataaaata caaataaaaa gaataaaaaa
      781 tttttatatt tttatatttg ttgttctgtt ataaaatgaa agaaattaga aactagtttc
      841 taaatcatga aaaatattga aaaaaaaaaa aaataagtgt taaaataaag aatgtaaacg
      901 tttacttcaa taaaggagct catatgttac tgcaaaaaga actaattcca atgatagaag
      961 ctaacttacc aaatatggca tatgctgaaa aagacattgc taaattcttc ttaaaacagc
     1021 aacctctgaa taattattca tgtaaggcat tgtgcgaatg ccttaatgta tccaaagcaa
     1081 cattgactcg atttgcgaaa aaatgtggtt ttaaaggttt tagacaattc attttcaaat
     1141 accaagagat gattcgtgag aaagaaaagt tagcattgta tacagaggct acagaaaaag
     1201 ttttatccga ctatgaagaa atgttgagaa aaacttacac ggttcttgat gaagttcaac
     1261 ttgagcgtat tgctgagatg atagaaactg ctgagcgtgt atatctttat ggtaaagtaa
     1321 gttctgttct tgctttacaa gaaatgaaga tgagatttat gcgtctcgga gtcattggtg
     1381 aagtattatc agacgaggat atgattttgt ggaatagctt attacttaat gaaaattgcc
     1441 ttgtcattgg agcatccgtt tcaggtcaaa ctgatattgt actagaaggt cttcaaaaag
     1501 ctgcggataa aggcgctaaa acagttttaa tgactacaag aaaatttgac gaagaagatt
     1561 gtttctttga tgaactattg ttattagctt cgaccaatca tctctcgtat ggcaatcgca
     1621 tatcacctca gtttccaata cttttaatta cagactgctt attctctcat tatctggaaa
     1681 gtccagagag acaatattat tacaatcaaa ctattatcca taaggaggaa taataaaatg
     1741 agtcaaattt ggactaaaga aaaatttata agtcaagtcc atggtggagt cattgtttct
     1801 tgtcaagctt tacccggtga acccctttat aatgaagaat ttagcttgat gccttttatg
     1861 gctaaagcag ctttagaggc aggagcagtg ggcattcgcg caaattctgt gcgtgatatt
     1921 aaagcaattc agaaagtagt agatttacca ataattggaa ttatcaaaag agattatcca
     1981 cctcaagaac catatattac tgctacgatg aaagaagtag atgaacttgt agaatgcgga
     2041 acaacagtca ttgcatttga tgcaacttta agaccaagat atgatggctt agttatcaat
     2101 gaatttatca aaaaaataaa agaaaaatat cctaatcaat tgttgatggc ggatgtaagt
     2161 aattttgatg aaggtctcta tgcatttaaa tcaggcgttg attttgttgg tacaacatta
     2221 tcagggtaca caagtacaag tgtacaatca gatgagcctg attttgaact aatgaaaaaa
     2281 ttggctgatt ttaatattcc ggtaattgcc gaaggaaaaa ttcattatcc agaacaatta
     2341 aaaaaagctt atagtttagg tgttaccagt gttgtcattg gtggagcgat tacacgtcca
     2401 aaagaaattg ctcagcgatt tattaatgtc atcaaataaa aaacaatata aaggagaaat
     2461 ctgatgaaag atttaactaa atacaaaggc gttattcctg cattttatgc ttgctatgat
     2521 gaaaatggtg aaattagcca agaacgtgta aaatctctgg tgcaatattt cattgacaaa
     2581 ggtgtaaaag gtatctatgt aaatggttct tcaggtgaat gtatttacca aagtgtagaa
     2641 gatcgtaaac aaattattga agctgttatg gaagttgcta aaggtaaatt aacagttatc
     2701 aaccatgttg catgtaataa cacgaaagat agtattgaat tggcaaaaca ttcagaaagt
     2761 gttggagtcg atgctattgc tgctatccca cctatttact tcaaattgcc agaatattca
     2821 attgcagcat attggaatgc aatgagtgaa gctgcgccaa atacagattt tatcatttat
     2881 aatattccac aattggcagg ggttgcgttg actggtagtt tgtatgcaac aatgcgtcaa
     2941 aatcctcgtg tgattggagt taaaaattct tctatgcctg tacaagatat tcaaatgttt
     3001 gtagctgcag gtggagaaga ttacattgta ttcaatggtc cagatgaaca atatcttggt
     3061 ggtcgcttga tgggagcaga agctggtatt ggtggtactt atggcgttat gccagatttg
     3121 ttcttgaaat tggaaagttt gattcaagaa cgagatttag atactgctaa aaaacttcaa
     3181 tatgctatca atgaagttat ctataagatg atatcaggta aggcaaatat gtatgctgta
     3241 gcaaaagagg ttttgcgtct aaatgaaaaa cttgatttag gttctgttcg tcaaccctta
     3301 gaagcattgg cagaaggtga cttggaagtt gcaaaacaag cagcagaact tattcaacaa
     3361 gcacgaaaag aatttttata ataaataaaa tcaatatgga gatatgttat gggcacaaca
     3421 ggatttacaa taattgactt aattatcttg attgtttatt tacttgcggt gttggttgca
     3481 ggtatctatt tctctaaaaa agagatgaaa ggaaaagagt tcttcaaagg agatggttcg
     3541 gttccttggt atgttacttc ggtatccatt tttgccacaa tgctcagtcc gatttccttc
     3601 ttgggactcg ctggtaactc ttatgcaggt agctggattt tatggtttgc tcaattaggg
     3661 atggtagtag ctattccact gacaattcgt tttatcttac ctatctttgc acggatagac
     3721 atcgatacgg catatgatta cttggataaa cgttttaatt ctaaagcact tcgtattatt
     3781 tcagcactct tgtttattat ttatcaattg ggacgtatgt ctatcattat gtacctccca
     3841 tcagctggtt tatcagtatt gacaggaatt gacatcaata ttttgattat tttgatgggt
     3901 gcagttgcaa ttgtttattc ttacactggt ggtctaaaat ccgtattatg gacagacttt
     3961 attcaaggtg tgattctgat tagtggtgtc gttttagctt tatttgtact gatggctaat
     4021 attaaaggtg gctttggtgc agtagcagaa acattagcaa acgggaaatt ccttgctgca
     4081 aatgaaaaac ttttcgatcc taacttgctt tcaaactcca tctttttaat tgtgatgggt
     4141 tcaggcttta caatcttgtc ttcctatgct tcatctcaag atttggttca acgttttact
     4201 acaacacaaa atattaagaa acttaataag atgttgttca caaacggtgt cttgtcactt
     4261 gcaactgcaa cagtctttta cttgattggt acaggcttgt acgtattcta tcaagtacaa
     4321 aatgcaggta gtgcagctag caatatccct caagaccaaa tctttatgta ctttattgca
     4381 taccagttac cagtaggtat cacaggtttg attttggcag cgatttatgc agcatctcaa
     4441 tcaactattt caacaggttt gaactctgtt gcaacttcat ggacattgga tattcaagat
     4501 gtcatttcta aaaatatgtc agacgatcgt cgtacgaaaa ttgcacaatt cgtatctcta
     4561 gcagtaggtt tattctcaat tggtgtttcc attgtcatgg ctcactcaga tattaaatct
     4621 gcatacgaat ggttcaatag cttcatggga cttgtacttg gtctacttgg tggtgtattt
     4681 attcttggat ttgtttctaa aaaagcaaat aaacaaggtg cttatgcagc gctgattgta
     4741 tcaactatcg tcatggtatt tattaaatac ttccttcctc caacagctgt gagctactgg
     4801 gcatattcat tgatttcaat ctctgtatca gtagtttcag gttatattgt atctgttctt
     4861 actggaaata aagtatctgc acctaaatat acaacgattc atgatattcc agaaattaaa
     4921 gcggattcaa gttgggaagt tcgtcactaa tagaaaagag aaaataatat gattatttct
     4981 gaacaatctg attttagacg atatgcttct atcaataaac atttttcaaa agtctgtgat
     5041 tttttagaaa atacaaattt gacagattta gttgatggaa aagttgatat tgacggggaa
     5101 aatgtttttg caaattgtat gatctatcta gctgatggag tcccaggaga tatttttgaa
     5161 actcataaga aatatttaga tattcatatt gttgttgaaa atactgaaaa aatggcagta
     5221 acttccccag ttcgtgccca gtcacgtgta ccttttagcg aagaaaagga tattgctttt
     5281 tacgatagca aagactatca aattgttgaa ttgcttccag ggaatatgtt ggtgactttt
     5341 gaagaagatc ttcaccaacc aaagattcat tgcaatgatg agcctgttaa aaaacttgtt
     5401 attaaagttt taaacgagga aaaataaaat gaaaaaaaat attaaacaat acgtaacctt
     5461 aggtactgca gtggtattat cagcatttgt tgctaactca gttgcagctc aggagactga
     5521 aacttctgaa gtatcaacac cagagttggt gcaacctgtt gcaccaacga ctccgatttc
     5581 ggaagtacaa cctaaatcgg gtaactcttc ggaagttact gtacaaccta gaacagtcga
     5641 aactactgtt aaggatccat cttctgcaac ggaagaaact cctgtcttag aaaaaaataa
     5701 tgttacttta acagggggcg gagaaaatgt tactaatgag ttaaaggata aatttactag
     5761 cggtgacttt actgtagtga ttaagtacaa tcagtctagt gagaaaggct tacaagctct
     5821 gtttggaata tctaattcta aacccggtca acaaaatagt tatgtagatg tgttccttag
     5881 agacaatggt gaattgggaa tggaagcgcg tgatacttct tccaataaaa atcacctagt
     5941 atccagacct gcttcagttt ggggtaagta caaacaagaa gctgtgacta acactgttgc
     6001 agtagtagca gattcagtca aaaaaacata ttctttatat gcaaatggta caaaagtagt
     6061 agaaaagaaa gtggataact tcttaaacat caaggatatt aaaggtattg attactatat
     6121 gcttggggga gtgaaacgtg caggaaaaac ggcgtttggt tttaacggaa cactagaaaa
     6181 tatcaaattc tttaatagtg cattggatga agaaactgtt aaaaagatga caacaaacgc
     6241 tgttactgga catttaattt atacggctaa tgatacaaca ggttctaact atttccgtat
     6301 tccagttctg tatactttta gcaatggtcg ggtattttca agcattgacg ctcgttacgg
     6361 cggaactcat gattttttga ataaaattaa tattgctaca agttatagtg atgataatgg
     6421 taagacatgg actaaaccaa aattaacatt ggcattcgat gattttgcgc cagtaccatt
     6481 agaatggcct cgtgatgttg gtggacgtga cttacaaatc agcggtggtg caacctatat
     6541 tgactctgtt attgttgaaa aaaataacaa acaagtactc atgtttgcgg atgtgatgcc
     6601 tgctggagta agttttagag aagctactag aaaagattca ggttataaac aaattgatgg
     6661 taattattac cttaaattaa agaaacaagg tgatactgat tacaattata ctattcgtga
     6721 gaatggtact gtatacgacg atcgtaccaa cagaccaact gaattttcag tagataaaaa
     6781 tttcggtatt aaacaaaatg gtaattattt gacagtagag caatattcgg tttcatttaa
     6841 aaataataaa aagactgaat atcgtaatgg gactcacgtc catatgaata tcttctacaa
     6901 agatgcattg ttcaaagtag tgccaacgaa ctatattgca tatatttcta gcaatgatca
     6961 tggcgaatct tggtctgcac caactttatt acctcctata atgggactta atcggaatgc
     7021 gccttatttg ggtcctggac gtggaatcat tgaaagctca actggacgta ttcttattcc
     7081 gtcttacact ggtaaagagt ctgcattcat ttatagtgac gataatggag catcttggaa
     7141 agttaaagtt gtgccacttc cttctagttg gtcagcagaa gcacaatttg tagaattgag
     7201 tccaggagta attcaagcat acatgcgtac aaataatggt aaaattgcat atttaacaag
     7261 tacagacgca ggtactactt ggagtgcacc ggaatatttg aaatttgttt caaatccaag
     7321 ttatggaaca caattatcaa tcatcaatta tagtcaactg attgatggta aaaaggctgt
     7381 cattttaagt actccaaact ccacaaatgg tcgtaaacac ggacaaattt ggattggtct
     7441 aattaatgat gataatacaa ttgattggcg ttatcatcac gacgttgatt atagtaacta
     7501 tggatactca tattcaacat tgacagagtt accaaatcat gaaattggat tgatgtttga
     7561 aaaatttgat tcatggtctc gtaatgaact tcatatgaaa aatgttgtac catatataac
     7621 atttaagatt gaagatctga aaaagaatta aagctgaaat ttgaaaatat ataaaaagag
     7681 gataaaaatt atggtaaatt acggtattgt tggagctgga tattttggag ctgatttagc
     7741 tcgctcaatg aacaaaattg aagatgcaaa agtggttgcg gtatttgacc caaatcatgg
     7801 agaagaagtt gctcaagagt tgggatcaga tgtttgtgca agtttagatg aacttgtagc
     7861 acgtgaagat attgattgtg tgatcgtagc ttcacctagc taccttcacc gtgaaccagt
     7921 tgtgaaagct gctcaacatg gcaaacacgt attttgtgaa aagccaattg cattgtctta
     7981 tgaagattgt aaagccatgg ttgacgcatg taaagaaaat aatgttatct ttatggctgg
     8041 tcacatcatg aacttcttta acggtgtaca ccatgctaaa gaattgatta ctcaaggtaa
     8101 aatcggtaaa gttctttatt gccatgccgc tcgtacaggt tgggaagaac aacaaccaac
     8161 tgtatcatgg aagaaacttc gttctcaatc tggaggacat ttgtaccatc atattcatga
     8221 attagattgc attcagttta tcatgggagg acttcctgaa aaagcgacaa tggtaggagg
     8281 caatgtatat cataaaggtg aaaactttgg tgatgaagat gatatgctca ttgtaaactt
     8341 agaatactct gatgatcgtt atgctgtttt ggaatatggt aatgctttcc gttggggtga
     8401 acactacgtc ttgattcaag gaactgaagg agctatcaaa cttgatttgt tcaatactgg
     8461 aggtactctt cgtgttaaag gtgaaggaga atcacacttc ttagttcatg aaactcaaga
     8521 ggaagatgat gaccgtacag ctatctatac cggtcgtggt atggatggag caattgcgta
     8581 cggtaaacca ggagtacgtt gcccattatg gttgcaaaca tgtattgata aagaaatgga
     8641 atatctacat gacatcatta aaggtggaga aattacagaa gaatatgaaa aacttctcaa
     8701 tggtgtagct gctttagaat caatcgctac cgctgatgca tgtactttat cagttaaaga
     8761 agatcgaaaa gtaagtcttt cagaaatcac aaatgcttaa cttttgtaaa acagaatagt
     8821 aaattcttat cattatataa tttctaaagt tctgtgatac aactcattga ataaagaaat
     8881 agagatggga ctgggataat gcccagtccc attttttatc aaaaagtaat gagatcaaaa
     8941 atgtgggagt gatgaaatga agattatagg gatcgatatt ggcggaacaa caattaaagc
     9001 agatttatac aatgagtttg gaacgagttt aaatcagttc aaagagatag aaacaattat
     9061 tgactatgat ttgggaacga accagatatt aaatcaggtc tgtgatttaa ttggtgagta
     9121 tattttaaat cattcaattg atggtgttgg gatttccact gctggagttg ttaatgctaa
     9181 tacaggagag atcatctata caggctatac aataccaggg tatatcggag taaactttac
     9241 tttcgaaata gaaaaacgtt ttgggttgtc tacttttgtt gaaaatgatg ttaattgtgc
     9301 tgcattaggt gaattgtgga agggacaagc caaagataag aaaaatgtag taatggttac
     9361 tattggaaca ggtataggag gcagtattat tgtcaatgga caaattgtta acggatttaa
     9421 ctatactgct ggtgaagtag gctatattcc tgtaggtaat tcggattggc aaagcaaagc
     9481 ctcaacaacc gcattgattc atttatatca aaagaaaagc ttgaaaacta atcaaactgg
     9541 gcgtactttc tttactgatt taagagctgg agataaagtc gctaaagaaa cttttgaaat
     9601 ttttgtagaa aatctaacaa aaggtttatt aacgattact tatctactta atccagaaat
     9661 tctcatatta ggaggtggga tcctggctaa aaaggatatt ttgttacctg aaattcaaag
     9721 ttctttagct aaaaatgcaa tggataatag gtttttgcct aaaaatcttg tggcagctac
     9781 attaggaaat gaagctggtc gtataggagc tgtaaaaaat ttcttagata gaatttctaa
     9841 taaatagtat gaaagataag gaggtgtcac aatgactaac tctgtatttt cgacaatgca
     9901 agatattgag aatgttgcaa ccgatattat aaaatcatat gataatgaga tttatactta
     9961 taaagctgtt tcccaagaag aattggaaaa actagaaaaa agttatgatg aaaaaagtca
    10021 cgaagaatta gtttcaatag aaagcaattt agaaatgaaa caacagaacc ttattgatga
    10081 ggttaataaa acaatcaagg aaaattatga aaagattcag tatatttcat caagtaggag
    10141 aggagaattt gtagaaaaaa ttattggtag ggtggtagaa aaatatggct attagtcaga
    10201 tgaaaagaat ttctctacta ttttctaaaa gtagtcttga tgatgtttta aaaactattc
    10261 aagaactaga gtcagtgcag ttccgtgatt taaaggtt
//
LOCUS       DAVBHS010000070        10152 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVBHS010000070 DAVBHS010000000
VERSION     DAVBHS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10152
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.070"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            118..1560
                     /gene="gtfA"
                     /locus_tag="VQ651_001239"
     CDS             118..1560
                     /gene="gtfA"
                     /locus_tag="VQ651_001239"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HET2754242.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTEILPEIHEHYSIQFKIADHDYYVYDFTLPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAREVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVSF"
     gene            1698..2891
                     /locus_tag="VQ651_001240"
     CDS             1698..2891
                     /locus_tag="VQ651_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET2754243.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            3578..5557
                     /locus_tag="VQ651_001241"
     CDS             3578..5557
                     /locus_tag="VQ651_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2754244.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            5624..7120
                     /locus_tag="VQ651_001242"
     CDS             5624..7120
                     /locus_tag="VQ651_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2754245.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            7120..8046
                     /locus_tag="VQ651_001243"
     CDS             7120..8046
                     /locus_tag="VQ651_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2754246.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSIRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            8055..9122
                     /locus_tag="VQ651_001244"
     CDS             8055..9122
                     /locus_tag="VQ651_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754247.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            9133..10059
                     /locus_tag="VQ651_001245"
     CDS             9133..10059
                     /locus_tag="VQ651_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754248.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
BASE COUNT         2990 a         1802 c         2127 g         3233 t
ORIGIN      
        1 ctacggtaag gcgacactga cgtggtttga agagattttc gaagagtata acatgaattt
       61 gatttacgaa gccagatcat atgagacttg gcttcaatta gaaaaaggag agtaatgatg
      121 ccaattcaaa ataaaaccat gttgattacc tattctgata gccttggaaa taatcttaaa
      181 gacttatatg ataatttgga agagcatttt ggagatgcta ttggaggagt tcacctttta
      241 ccatttttcc catcaacagg tgatcgtgga tttgcgccag ttgactacga cgaagtggac
      301 tcagcttttg gtgattggga ggatgttaag cgtttaggtg agaaatatta tcttatgttt
      361 gattttatga ttaatcatat ttctcgtcaa tccaagtatt ataaggacta tcaagaaaaa
      421 catgaagcca gtgaatttaa agatctcttt ttaaactggg ataagttttg gccagaaaac
      481 cgtccgacac agtctgatgt agatttaatt tacaagcgta aggatcgtgc accaaagcaa
      541 gagattgtgt ttgaagatgg ttcagtggaa catttgtgga atacctttgg tgaggagcag
      601 attgatcttg atgtgaccaa agaagtaact atggaattta tccgtaagac cattcagcac
      661 ttggcaagta atgggtgtga tttgattcgt ctagatgcct ttgcttatgc agtgaagaaa
      721 ttggatacta atgatttctt tgtggaacca gatatttggg atttattgga caaagttcga
      781 gatatcgctg ctgagtatgg gacagagatt ttacctgaga ttcatgaaca ctattcgatt
      841 cagtttaaaa tagcagacca tgattactat gtttatgatt ttactcttcc aatggtgaca
      901 ctttatactc tttacagttc cagaacagag cgtttggcta agtggttgaa gatgagcccg
      961 atgaagcaat ttacgacgct agatacccat gatgggattg gagtagtaga tgtcaaggat
     1021 atcctgaccg atgaggagat tgactatgct tcaaatgaac tctataaggt tggagccaat
     1081 gtcaaacgta agtactctag tgccgagtat aacaacttag atatctacca aatcaattca
     1141 acctactatt cagcgcttgg agatgatgat gtcaagtatt ttctcgctcg tctaattcaa
     1201 gcttttgccc caggtattcc tcaggtttac tatgtgggtc tattagcagg caagaatgac
     1261 ttgaaattat tagaagaaac taaagaaggt cgaaatatta atcgtcatta ctatagcaac
     1321 gaggaaatag caagagaagt gcaacgacct gttgtgaagg cccttctcaa tctattttct
     1381 ttccgtaacc gttcagaagc ctttgatcta gaagggacta ctgagataga gacaccaaca
     1441 gctcacagca ttgtaatcaa acgtcaaaat aaagataagt ccgtaacagc agtagcagaa
     1501 attgatttgc aaaatcagac ttatcgggta attgagaatg gagttgaagt atcattttga
     1561 agccttgata tggtggataa aatagggttt ttattttgga aaacgtttcc tttgttttca
     1621 aattgctaaa aaagtggtac aatataggat agcttactat tatctgaatc agctgatttg
     1681 gagagaaagg attcattttg aaatcaatag gctttattga aaagctgaag gggttgtcta
     1741 gtaaagagct gattttattg ggaattatcc taagtatctt tttacccttt tatctttttg
     1801 tagttgtact ctgtttatat attatcagtt tgatttttac aggagacatg aaaagtattc
     1861 ttcagaaaat gggggagcat ccgatgctgc ttctttttct tagctatagt actgttatat
     1921 ccattcttgc acaaaattgg atgggtcttg tggcttcagt aggaatgttt ctatttacta
     1981 ttttcttttt gcactatcag tcgattttat cccataaatt ctttcgattg attttgcagt
     2041 tcgtcttgtt tggtagtgtc ttgtcagctg cttttgccag tttagaacat ttccaaattg
     2101 tgaagaaatt taactatgct tttctttcac ccaatatgca ggtgtggcat cagaaccggg
     2161 cagaagtgac cttctttaat cctaattatt atggaattat ttgttgtttc tgtattatga
     2221 ttgctttcta tctgtttaca acgaccaagt tgaattggtt gaaagtattc tgtgtgattg
     2281 caggctttgt taatctcttt ggtttgaact ttactcaaaa tcgaactgcc tttcctgcta
     2341 ttatcgctgg agcaattatc tatctcttta cgactattaa aaactggaag gccttttggc
     2401 ttagtattgg ggtcttcgcg attggtttga gtttcctctt ttctagtgat ttgggagttc
     2461 gaatgggtac tttagactct tctatggaag aacgcatttc tatctgggat gctgggatgg
     2521 ccttgtttaa gcaaaatcct ttttggggtg aagggccatt gacctatatg cactcttatc
     2581 ctcggataca tgctccttat catgaacatg cccacagtct ttatattgat acgattctga
     2641 gttacggaat tgtgggtacc attttattag ttttgtcttc tgttgctcct gttcgcttga
     2701 tgatggatat gagtcaggag tcggggaaac gtccgattat cggcctttat ctatctttcc
     2761 ttacagtggt tgctgtgcac ggaatttttg acttggctct cttctggatt cagtcaggct
     2821 ttattttctt gctagttatg tgcagtattc cattggagca tcgaatgttg gtatcggaca
     2881 tgacggatta agttttataa gtttgaaatc ttctaccttg ggtaggagat ttttttatac
     2941 gatgggcatg tcgtgcatct gaggtgtaag tcctctgtag ggatccacta ccggtgaata
     3001 caatagcgct ccccaagcct gactatcgtg aggtagcgga ttaaaatggt ctggggatag
     3061 atcgttttaa gtctgacgca ggaaataaga attgtcagag aaagcaatag tgaaatcgta
     3121 ggctatgaat agaaacgtga tagcgaggag catatagtgg ataaggtagc gagaaatttt
     3181 aaagtataaa agatagtctt aatctatata tgtaaatcac aaaagtaatt gaattcggaa
     3241 taaggttggt gtgaaaagac atagtaaact gaatcttgaa tactagaatg atacttctac
     3301 aatattgtta taatttaatt tgaattccaa aatcacattg ttccgtccgt atttcatttt
     3361 actgtatgtc ttcctaaact ccaaagtttc tgcgtcaaat ttcctatttt cactgtagtc
     3421 tttgacactt cgaacgacaa tttgaatcat atattcaaaa aaaaatgaaa aatttgtgcc
     3481 aacaagccta aatattctga aaatttgttt gatttttaat ggataatgtg atataatggt
     3541 tgtcaatcta taattcacat aaacagagga gtagaaaatg aaaaaaaatc gtgtatttgc
     3601 tacagcaggt cttgttttat tagcagcagg tgtacttgca gcatgcagtt cttcaaaatc
     3661 atctgattca tcagccccta aagcttatgg ctatgtttat acagcagacc cagaaacctt
     3721 ggactacctg atttcaagta aaaatagtac aacagtagtg acttcaaatg ggattgatgg
     3781 tttattcact aacgataatt acggtaatct tgctcctgca gttgcagagg attgggaagt
     3841 ctctaaggat ggtttgacct acacttataa gattcgtaaa ggggttaaat ggtttacctc
     3901 tgatggagaa gaatatgcag aggtgacggc taaagatttc gtgaacggtt taaaacacgc
     3961 agcagataaa aaatcagaag ctatgtattt agctgaaaat tcggttaaag gcttggcaga
     4021 ttatctatca ggaacttcaa cagatttttc aacagttggt gtcaaggcgg ttgatgatta
     4081 tacgttacaa tacactttga accagcctga accgttctgg aactctaagt tgacctattc
     4141 tattttctgg cctctgaatg aagaattcga aacatcaaaa ggaagcgatt ttgctaaacc
     4201 aacagatccg acatccttgc tttataatgg tccattcttg ttgaaagggt tgactgcaaa
     4261 atcttctgta gagtttgtaa aaaatgagca atattgggat aaagaaaatg tccacctaga
     4321 tactatcaat ctagcttact atgatggatc agatcaggag tcgctagagc gtaacttcac
     4381 tagtggagct tatagttatg cccgtcttta ccctaccagc tccaactatt ctaaggttgc
     4441 agaagaatac aaggacaata tctattacac acaatcaggc tctgggattg ctggtctggg
     4501 tgtgaatatt gatcgccaaa gttacaacta tacttctaaa actacagatt cagagaaagt
     4561 agctactaag aaggcattgc ttaacaaaga tttccgtcaa gccttgaatt ttgctcttga
     4621 tcgctcagct tactcagctc aaatcaatgg taaagatgga gcagctttag cagttcgtaa
     4681 tttatttgta aaaccagact ttgtttcagc tggtgagaag acctttggtg atttagtcgc
     4741 tgctcaactt cctgcttatg gtgatgagtg gaaaggtgtg aatttagctg atgggcagga
     4801 tggtttattc aatgctgaca aggccaaggc agagtttgcg aaagctaaga aagctttaga
     4861 agcagacggc gttcagtttc ctattcatct ggacgttcca gtagaccaag catcaaaaaa
     4921 ctacatatct cgtattcagt cctttaaaca atctgtagaa acagttcttg gtgttgaaaa
     4981 tgtcgttgtt gatattcaac aaatgacaag tgatgaattc cttaatatta cttactatgc
     5041 tgccaatgct tcatctgagg attgggatgt atcgggagga gtttcatggg ggccagacta
     5101 tcaagaccca tctacttacc tggatatttt aaaaacaact agcagtgaaa ctacaaaaac
     5161 atatttagga tttgataatc caaatagccc ttcagtagtt caagttggtt tgaaagaata
     5221 cgataaatta gttgatgaag ctgccaaaga gacaagcgac ttgaatgtcc gttatgaaaa
     5281 atatgcagcg gctcaagcat ggttgacaga tagttcactc tttattcctg ctatggcttc
     5341 ttctggtgca gcaccagtgc tttcacgaat tgttccattt actggagctt ctgcgcaaac
     5401 aggctctaag gggtcagatg tttacttcaa atatttgaaa ttacaagata aagtggtgac
     5461 taaggaagag tatgaaaaag ctcgtgaaaa atggttgaaa gaaaaagctg aatcaaatga
     5521 gaaagctcaa aaagaattgg caagtcatgt gaagtaaata atttctaata gaactttctc
     5581 tccatgagga gaaggttctt ttgggatttt taaaggaaat aatatgaaaa aatatatttt
     5641 tatgcgtgtt ttgcggtcat tggtttcgat tttcttagta acgactttga cctacacgat
     5701 tatctatacc ttggttcctc gaaaattgat tttcaagcag gatcctaact ataataaaat
     5761 tgcaacaacg gctgataaac gtgataacta tgaaaatact gtgtttgagc gtatgggcta
     5821 cattgagtat tacgatacta aagagttgca agaaaaggca agtagcatgg attcttctgt
     5881 aacagtagaa gcaaatgcga ccaataaagc tatttatgaa aagtacatca atcaattagg
     5941 tcatggttgg actttgggag aatttactga aagtggtcaa ttctatgcta ctcgtgaaat
     6001 tccaattttt gaacgtgttt ttcacttcta tgctaacttg attgacattg accatacaaa
     6061 taaaatccaa gaccctgaaa atccagactt gaaacgctac cttcgttttg aaaatgatcc
     6121 agctatcgga tggtcattgg tcggttcagg aactaaacat aaatatctct tgtactttaa
     6181 cagtcagttc ccatttgttc atcaaaactt tgtgaacttg aatttaggtg actcttaccc
     6241 aacctatgct aatacaccag ttcttcaggt tattactcaa ggtcaaggac aaaccaaaac
     6301 tgcccaagtt cagttcccaa caggtaagaa aacgtcttct gtaaatattt actcaagaac
     6361 ctacaagtca cctagtcagg ctgactctcg tgaagtagct agctatggga aagatgatcc
     6421 ttatacagcg actgaaagta attaccaata tccatctatg attgtcagct ctgctattac
     6481 tggtttgatt ggtttggttc ttgcctatgc tcttgccgtg ccacttggtt cagccatggc
     6541 tcgtttcaag aacacttgga ttgatagcct ctcaacaggg gctttgacct tcttgcttgc
     6601 tcttccaacg attgccttgg tttacatcgt tcgattgatt ggatcatcta ttgcccttcc
     6661 agattcattc cctatcttgg gagctggaga ttggcgttct tacgttttac cagcagtcat
     6721 ccttggtttg ttgggtgctc ctggtacagc catttggatt cgtcgttaca tgattgactt
     6781 gcaatctcaa gactttgttc gtttcgctcg tgcaaaaggt ttgtctgaaa aagaaatttc
     6841 aaacaaacac atctttaaaa atgccatggt tccgctggtt tcaggaattc ctggtgccat
     6901 cattggggtt atcggtggtg caacccttac tgaaacagtc ttcgccttcc caggtatggg
     6961 taaaatgttg attgactctg taaaagcatc taataactct atggtcgttg gtcttgtctt
     7021 catctttaca tgtatttcta tcttctcacg tcttttggga gatatttgga tgactattat
     7081 tgacccacgt attaaattga ctgagaaagg aggcaaataa tgtctacaat cgataaagaa
     7141 aaatttcagt ttgtaaaacg tgacgatttt gcctctgaaa ctattgatgc gccagcatat
     7201 tcttactgga aatcagtgtt taaacaattt atgaagaaaa aatcaactgt agtcatgttg
     7261 ggaatcttgg tagccatcat tttgataagt ttcatctacc caatgttttc taagtttgat
     7321 ttcaatgatg tcagcaaggt aaacgacttt agtattcgtt atatcaagcc aaatgcggag
     7381 cattggttcg gtactgacag taacggtaaa tcgctctttg acggtgtctg gttcggagct
     7441 cgtaactcca tcctcatttc tgtgattgcg acagtgatta acttggttat cggtgttttt
     7501 gtcggtggta tttggggtat ttcaaaatca gttgaccgtg tcatgatgga agtttacaac
     7561 gtcatctcaa acatcccacc tcttttgatt gttattgtct tgacttactc aatcggagct
     7621 ggattctgga atctgatttt tgccatgagc gtaacaacat ggattggtat cgccttcatg
     7681 atccgtgtgc aaatcttgcg ctatcgtgac ttggaataca acttggcatc acgtactttg
     7741 ggaacaccaa ccttgaagat tgttgccaaa aatatcatgc ctcaattggt atctgttatt
     7801 gtgacaacca tgactcaaat gcttccaagc tttatctcat acgaagcctt cttgtctttc
     7861 ttcggtcttg gattaccgat tacagtgcca agtttgggtc gtttgatttc ggattattca
     7921 caaaacgtaa caaccaatgc ttacttgttc tggattccat tgacaaccct tgtcttggta
     7981 tccttgtccc ttttcgtagt tggtcaaaac ttagcggatg ctagtgatcc acgtacacat
     8041 agataggagt agaaatgaca aaagaaaaaa atgtaatttt gactgctcgc gatattgtcg
     8101 tggaatttga cgttcgtgac aaagtattga cagccattcg cggcgtttcc cttgaactcg
     8161 ttgaaggaga agtattagcc ttggtaggtg agtcaggatc aggtaaatct gttttgacaa
     8221 agaccttcac aggtatgctc gaagaaaatg gtcgcattgc ccaaggtagt attgactacc
     8281 gtggtcagga tttgacagct ttatcttctc acaaggattg ggaacaaatt cgtggtgcta
     8341 agattgcgac tatcttccag gacccaatga ctagtttgga ccccattaaa acaattggta
     8401 gtcagattac agaagttatt gtaaaacacc aaggaaaaac agctaaagaa gcgaaagaat
     8461 tggccattga ctacatgaat aaggttggca ttccagacgc agatagacgt tttaatgaat
     8521 acccattcca atattctgga ggaatgcgtc aacgtatcgt tattgctatt gcccttgcct
     8581 gccgacctga tgtcttgatc tgtgatgagc caacaactgc cttggatgta actattcaag
     8641 ctcagattat tgatttgcta aaatctttac aaaacgagta tcatttcaca acaatcttta
     8701 ttacccacga ccttggtgtg gtggcaagta ttgcggataa ggtagcggtt atgtatgcag
     8761 gagaaatcgt tgagtatgga acggttgagg aagtcttcta tgaccctcgc catccatata
     8821 catggagtct cttgtctagc ttgcctcagc ttgctgatga taaaggggat ctttactcaa
     8881 tcccaggaac acctccgtca ctttatactg acctgaaagg ggatgctttt gccttgcgtt
     8941 ctgactacgc aatgcagatt gacttcgaac aaaaagctcc tcaattctca gtatcagaga
     9001 cacattgggc taaaacttgg cttcttcatg aggatgctcc aaaagtagaa aaaccagctg
     9061 tgattgcaaa tctccatgat aagattcgtg aaaaaatggg atttgcccat ctggctgact
     9121 aggaggaagg aaatgtctga aaaattagta gaaatcaaag atttagaaat ttccttcggt
     9181 gaaggaagta agaagtttgt cgcggttaaa aatgctaact tctttatcaa caagggagaa
     9241 actttctcgc ttgtaggtga gtccggtagt gggaaaacaa ctattggtcg tgctatcatc
     9301 ggtctaaatg atacaagtaa tggagatatc atttttgatg gtcaaaagat taatggtaag
     9361 aaatcgcgtg aacaagctgc ggaattgatt cgtcgaatcc agatgatttt ccaagaccct
     9421 gctgcaagtt tgaatgaacg tgcgactgtt gattatatta tttctgaagg tctttacaat
     9481 caccgtttat ttaaggatga agaagaacgt aaagagaaag ttcaaaatat tatccgtgaa
     9541 gtaggtcttc ttgctgagca cttgactcgt taccctcatg aattctcagg cggtcaacgt
     9601 caacgtatcg gtattgcccg tgccttggtc atgcaaccag actttgttat tgcagatgag
     9661 ccaatttcag ccttggacgt ttctgtacgt gcccaagtct tgaacttgct caaaaaattc
     9721 caaaaagagc tcggcctgac ctatctcttc atcgcccatg acttgtcggt tgttcgcttt
     9781 atttcagatc gtatcgcagt tatttacaag ggtgttattg tagaggttgc agaaacagaa
     9841 gaattgttta acaatccaat tcacccatat actcaagcct tgctttcagc ggtaccaatc
     9901 ccagatccaa tcttggaacg taagaaggtc ttgaaggttt acgacccaag tcaacacgac
     9961 tatgagactg ataagccatc tatggtagaa atccgtccag gtcactatgt ttgggcgaac
    10021 caagccgaat tggcacgtta tcaaaaagga ctaaactaat aatggtttta taatttccat
    10081 gtcaattttt atgggaatta taaaactttt ttgttggact gatttgaaaa actttgaaag
    10141 cttcctgaaa ga
//
LOCUS       DAVBHS010000071        10097 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVBHS010000071 DAVBHS010000000
VERSION     DAVBHS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10097
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.071"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..335)
                     /locus_tag="VQ651_001246"
     CDS             complement(<1..335)
                     /locus_tag="VQ651_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754249.1"
                     /translation="MEYLFKMIILLPCFYFFSWIEKANRDSKIFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            complement(475..810)
                     /locus_tag="VQ651_001247"
     CDS             complement(475..810)
                     /locus_tag="VQ651_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754250.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(901..2211)
                     /gene="der"
                     /locus_tag="VQ651_001248"
     CDS             complement(901..2211)
                     /gene="der"
                     /locus_tag="VQ651_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET2754251.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(2225..2938)
                     /locus_tag="VQ651_001249"
     CDS             complement(2225..2938)
                     /locus_tag="VQ651_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HET2754252.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(2935..3831)
                     /gene="dnaI"
                     /locus_tag="VQ651_001250"
     CDS             complement(2935..3831)
                     /gene="dnaI"
                     /locus_tag="VQ651_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HET2754253.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(3832..5001)
                     /locus_tag="VQ651_001251"
     CDS             complement(3832..5001)
                     /locus_tag="VQ651_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HET2754254.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFNPKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRER
                     GQKTKTQKQNQTAPAKTNIPKWSNPDYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(5002..5475)
                     /gene="nrdR"
                     /locus_tag="VQ651_001252"
     CDS             complement(5002..5475)
                     /gene="nrdR"
                     /locus_tag="VQ651_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HET2754255.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            5615..5980
                     /locus_tag="VQ651_001253"
     CDS             5615..5980
                     /locus_tag="VQ651_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2754256.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMIH
                     FGYEKEELPGVVSDYIKGV"
     gene            5985..6680
                     /locus_tag="VQ651_001254"
     CDS             5985..6680
                     /locus_tag="VQ651_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754257.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            6697..7464
                     /locus_tag="VQ651_001255"
     CDS             6697..7464
                     /locus_tag="VQ651_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754258.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(7817..9016)
                     /locus_tag="VQ651_001256"
     CDS             complement(7817..9016)
                     /locus_tag="VQ651_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2754259.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLEQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(9013..9906)
                     /locus_tag="VQ651_001257"
     CDS             complement(9013..9906)
                     /locus_tag="VQ651_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754260.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
BASE COUNT         2931 a         2246 c         1844 g         3076 t
ORIGIN      
        1 caatgccttc cgcctttaga cttccctagt tcccgtctca agtcaagcat ttttttgaaa
       61 cagatatagt ctaaccagtc actagcaaaa gcgataagta gtaataatag ccaaataccc
      121 cataacttga tatctgtcaa atttctcaag acgatattga aaaacagaac tgaaaaaact
      181 gtccaagcaa ggctaaaaag agcatagagg gtgatgtaaa tccagtaaaa atagtaaaaa
      241 attgggaaaa tcttactatc tctgtttgcc ttttcaatcc agctgaaaaa gtaaaaacag
      301 ggtaataaga ttatcatttt aaacaagtat tccatcacaa acctcctctt ttaaattgtt
      361 ttctttagtt tcgttctatt aaatgttatt accaatataa accttggaat agcgtgtaat
      421 aaatgatttt agaggtacca taagccacct cctacaaata gaaaccgata taaatcaatg
      481 ccttccaccc ttagacttcc ctagttcctg tctcaagcga aacatttctt tgaaacagga
      541 ataagttaac caattcatac caatagctag cagaataaaa agaaaccaaa tgccccataa
      601 cttgatatct gtcacatttc tcaagacggt attgaaaaac agaactgaaa caactgtcca
      661 agcaaggcta aaaagagaat agaaggggat gtaaaaccag taaaaataat aaaaaattgg
      721 aaaaaactta ctatttctgt tggccttctc aatccagtta tcaaaataaa agtacggtgc
      781 taaaagtaag aatttaaaca aatgttccat caccgacatc accctccttc ttttgatagc
      841 gttttctatt attttattat atcaaaaaaa tccggaactg tcattccaga ttctactttt
      901 ttatttgcgt tttcttgcga tgagatgaat cggtgttccc tcaaaaacaa aggccttgcg
      961 gatttgattt tccaagaaac gcaggtaaga aaagtgcatg agttcttctt cattgacaaa
     1021 gatgacaaag gttggtggtt tggttgccac ttgggtcgca tagaaaatct tgagacgttt
     1081 tcctttgtct gtcggtgttg ggttgatggc aatggcatcc atgatgacat cgttcaagac
     1141 agctgatgga atacgtgtat tttgactttc gctgatttgc ttaatcatct caggaagttt
     1201 gtggagacgt tgcttggtta aagctgatac aaagataatc ggtgcgtaag gcaggtattg
     1261 gaactgctca cggatatctt cttcccagtt tttcatagtg tggttatctt tttcaagcgt
     1321 atcccacttg ttgaccacga taatcatccc tttaccagct tcatgggcaa atcctgcgat
     1381 acgcttgtcg tactcacgaa tgccttcttc cgcattgatg accatcaaga ccacatctga
     1441 acggtcaata gcacgcatgg cacgcataac agagtatttc tcagtatttt cataaacctt
     1501 accagactta cgcataccag ccgtatcaat catggtaaac tcttgaccat ctgtatctgt
     1561 aaagtgggta tcaatggcat cacgagttgt tccagcaaca ggactagcaa taacacggtc
     1621 ttctcccaag atagcattga tcaagcttga ttttccaacg ttaggacgac caatcaagct
     1681 aaacttaatg acatctggat tttcttcctc atattcattt ggaagatttt ctacgatcgc
     1741 atctagcaca tcccctgtac cgattccatg gacagatgag ataggcaatg gttcacccaa
     1801 accgagagca tagaaatcat agatatcatt tctcatctca gggttgtcca ccttgttgac
     1861 tgcgaggata actggtttgt gggtcttata aagcttacgg gctacgtatt cgtctgcatc
     1921 ggtaattcct tccttaccgg acacgacaaa gacgataaca tctgcttctt ccatggcaat
     1981 ttctgcctgg tgcttgattt gttccatgaa aggagcatcg acatcatcaa ttcctcctgt
     2041 atcaatcatg ctaaaagaac gattgagcca ctcacccgtt gcataaatac ggtcacgtgt
     2101 cactccttcg acatcttcta caatggagat tcgctcacca gcgatccgat taaatagggt
     2161 tgatttccca acattgggac gtcctacaat ggcaatagtt ggtagggcca taatttctca
     2221 ctttctacaa taatttcttc tgttcaagat tttttctagt tgagcttggt tcagcttgac
     2281 caaactgttc tgctaggcgc tgactccagc ttgtggtcgc acgcgcccca gcatagtcag
     2341 cctgaacacg gtcataagct tggattgcct cagttgactg ttcttggtat tcttcctcaa
     2401 agacaacatt ctctagtggc agtctcggtt tcatatcatg atgttgattt ggcacaccca
     2461 gtgccatccc aaagacagaa taggtgtagt caggtaggtt aaagagctct gccacttctt
     2521 cagacttgta tcgaaccaaa ccgataatca caccaccata gcccaagctt tcagctgcca
     2581 acaaggcgtt ttgtccagca agagctgcat cgaccgaact aatcaagaga ccttccacac
     2641 cttggggttg gaaggtgtcg gtatgaagtc gggctccctt ttctgctcgg ttcaaatctc
     2701 cgacaaagag aaggaaaaca gcagactggc gaatggcttc ttgaggtacc aattcataca
     2761 aggcatcttt cttctcttga cttcgtacca caatcacaga gtaggattgg aaattcttcc
     2821 aagatgatgc catctgggct gctgtcaaaa tctcatttaa gtctacttgg ggaatttctt
     2881 gctctttaaa cctgcgcact gaagtatgag ccttcatcaa tttaatggtt tctgtcatcg
     2941 acggtttact ccttctaaac gagtctcctc agccaaataa cggatgcgtt ccatgacccg
     3001 tctggcttcc caggtttcgt catttccatg tttcactttc gcaaaatgct tctccaaatc
     3061 ttcaaagttg aagttggatg tgaaaaaggt cggtaaattt tcctgcatcc gatattggag
     3121 aatgacctgc aggatttcgt cacgcaccca agcggttgat tgctcggcgc caatatcatc
     3181 taaaatcagg acctcagaca gcttaatctc atccaccaag gtcttaacat tgccatcact
     3241 gatagcattt ttgacatcaa tgacaaagct aggatagtgg aggagagttg atgaaacacc
     3301 acgtttttct gataaatcat gagctaaggc cgccaccatg aaacttttac ccacaccaaa
     3361 gtctccatat aagtaaagac cttttcgaat agctggatat tgctccacaa aggctaatag
     3421 tttttcaaaa actggcaagc gtcccaaatc atccaaatca acttgagcca aactagcttt
     3481 tttgagactg gctggtagat tgattagctt gagacgattc ttaatagccg cttctttttc
     3541 agccgcgatt agctcaggag tttcttcata tgaaacatct gcatagccat gattcttaac
     3601 caaaatcggc ttgtagcctt tggcaatata atccgtatcc ccacggagaa acttgtcacg
     3661 ctcggtgatg tactgattaa acttggagat actgcgattt aattcctctg gagtcaagga
     3721 ttcttgctgg ataaaagccg caacatcagg gtccttcatg attttctgga ccaaatcttg
     3781 ataataaaaa cggctgggtt gacgtttgag tacgtctccg acactttcca tctaatctcc
     3841 tcctttttct aatcgagcta atagttcttg tttcttacgt tctagttcca gacgagtttc
     3901 ctcgctggtt tcattcttat aatctgggtt actccattta ggaatattgg tttttgctgg
     3961 ggcagtctga ttctgttttt gtgtttttgt tttctgccct cgctcacgaa ttcgtaaaac
     4021 ggcctcttct gctgaatgta tcttttgata ggcatagtca ttagccacct tcatggcata
     4081 tttctcattg atgtttgccg aatccacctt attaaaggtc aacaagagaa tgatattgat
     4141 gacttcgtcc agcaagccca agccagccat ctgttgcaaa agttctcttt ctgtttgggt
     4201 aatggtcccc ttgcgtgttt gcttgatttc tgctaagaac tgcagggcag tcttactttt
     4261 agcttccttg ataatggttg cttccttagg attaaagtca gaggaaactg gtttttgagc
     4321 aattttttca cgcatgcgtt tggttgaaat aacctgagaa acagctgttg acttggccaa
     4381 ttgataggtt tcaaaccaag tccatttctt ctcctcggca atagcaaaga ggtttaagac
     4441 atcggactgc tcatccgcaa aacgaagtcc atctcgagcc atcagctggc gaaaatgttc
     4501 caagtcaaaa tcattggcca ctttcttctt gagaccaagg tcttcttgac tgcctagttc
     4561 tgccaattct ggaaagactt gattgagtga gacaggtatt tcttcaccat cagcactttc
     4621 aactttcaaa tcctccacag cagagtcgcc aatctttttc tctaagagtc tgcgataaac
     4681 aggatgctcc aagaagtctt gactagatag aggagcatgg agggctagct gataaacatc
     4741 ccccttttga tagagggtca agagattaaa agcagataag attttcaatg attttatcag
     4801 tctatccatc ccaaagttga gatgattgag gatacttgaa aaaagatatt cctttctacc
     4861 attatcccaa aaactgattg tataaagata aaggctcagt gcctcctgac cgataatcgg
     4921 gaggtagcac tgtaccagag atgaggtatc ttgcgacacc cgattattct ttagataaga
     4981 aaaacggtca attggcttca tttatctttc ctttttcttt ttagaggact gggtgatttg
     5041 ttggagcaag ctctctaact cactgacatc cttaaaacta cgatagacac tagcaaaacg
     5101 tacataggta atctcgtcca attcagccaa ctcctccatg acgagtgaac caatgtcctc
     5161 actttgaatt tcattttcat ttcgaccacg gagtttctgt tcgatacgat tgactaccat
     5221 gttgatttca tcacttgaca caggacgttt ctgggctgag cggataatcc cattaaagat
     5281 tttatctctg gagaattgtt cccgtgtacc atctttttta acaaccacta aggttctttc
     5341 ttctactcgt tcgtaggttg taaaacggtg ttggcattcg tcgcactcac gtcttctacg
     5401 aatggtgttc ccttcttctg cttggcgact atcgataaca cttgacttgg tagccccaca
     5461 ttttggacaa cgcatccttt ccctccttat cgttttcttt tcattatacc attttttaag
     5521 cgattcccaa aacaattctt ctttttgctt gacaagtttt ttgttttgtt gtattattta
     5581 gttaagacaa aaggataaga gaaaggagac taagatgtcc tggacatttg acaacaaaaa
     5641 acccatctat ttacagatta tggagaaaat caagcttcag attgtttccc atacactgga
     5701 acccaatcaa caacttccaa ccgtgaggga gctagctagc gaggctggtg tcaatcccaa
     5761 taccatccaa agagccttat cagaccttga acgagaagga tttgtctaca gcaagcgaac
     5821 aactggacga tttgtgacta aggataagga gctaatcgct cagtcacgca aacaattatc
     5881 agaagaagaa ttggaacact tcgtttcctc catgatccat tttggctatg aaaaagaaga
     5941 actaccaggc gtagtcagtg attatattaa aggagtttaa gcctatgtca ttactagtat
     6001 ttgaaaatgt atccaaatca tatggagcaa caccagccct tgaaaatgtt tctcttgaca
     6061 ttccagctgg aaaaattgtc ggccttcttg ggccaaacgg ctcaggaaaa acaaccctga
     6121 ttaaactaat taatggcctc ttacaaccag atcaaggacg tgtcctcatc aacgacatgg
     6181 acccaagccc agcaaccaag gccgttgtag cttatttgcc tgatacgacc tatctcaatg
     6241 agcaaatgaa ggtcaaagaa gccctaacct acttcaagac cttctataaa gatttcaatc
     6301 ttgaacgcgc tcatcatcta cttgcagacc tgggcattga tgaaaatagt cgtctcaaga
     6361 aactatcaaa aggaaacaaa gaaaaggttc aactgatttt ggttatgagc cgtgatgctc
     6421 gtctctatgt tttggatgaa cccattggtg gggtggatcc agcagcccgt gcttatatcc
     6481 tcaataccat tatcaacaac tactcaccaa cttctaccgt tttgatttct acccacttga
     6541 tttctgatat cgagccaatc ttggatgaaa ttgtcttcct aaaagacgga aaagtcgtcc
     6601 gtcaaggaaa tgtagatgat attcgctacg agtcaggtga atccattgac caactcttcc
     6661 gtcaggaatt taaggcctaa gcaaaggaga ttatttatgt tttggaattt agttcactac
     6721 gaatttaaaa atgttaacaa gtggtattta gccctctacg cagccgtgct agtcctttct
     6781 gccctcatcg gaatacagac acaaggcttt aaaaatctac cttaccaaga aagtcaggct
     6841 actatgctac tttttctagc tacagtcttt ggtggcttga tgcttacact tgcgatttca
     6901 accattttct tgattattaa acgcttcaaa ggtagtgtct acgaccgaca aggctatctg
     6961 actttgacct tgccagtttc tgaacaccat atcatcacag ccaaactaat cggtgccttt
     7021 atctggtcat tgattagcac cgctgtattg gctctaagtg ctgttattat tctggcttta
     7081 acagctccag aatggattcc tctttcttat gtgattacat ttgtagaaac acatctccct
     7141 cagatctttc ttacaggtat atccttccta ctaaatacca tttcaggaat cctctgcatc
     7201 tacctggcta tttccattgg acagcttttc aatgaatacc gtacagcact cgctgttgca
     7261 gcctacattg gtatccaaat cgtcattgga tttattgaac ttttcttcaa tcttagttct
     7321 aatttctatg tcaattcact ggtaggactc aatgaccatt tctatatggg agcaggtata
     7381 gccattgtta aagaactcat attcatagct atcttttatc tcggaaccta ctacatcttg
     7441 agaaataagg ttaatttgct ttaaataatt tttacctaga tatgtaacat actcatagaa
     7501 caaaagagac caggcaaaaa gtctttaaaa ttagaaaacg catagtatca agtgttaaaa
     7561 atccttgata ttatgcgttt tttgtgagct gacttatttc ctttcactat atcgcaaaat
     7621 gaaataagaa cggaacgatg ggattttgga attcaaatca atttataaga atgttttaga
     7681 agtaatatta tcctattcca gattcagttc actatacaat tgagttttca agcaacctgt
     7741 ttacataatg tgtacatcat taggttcgtg attccaccct tttcaccttt aaaaacctcg
     7801 ctttcgcaag gctcttctat ttataagata aggcacgttt aaaggttttc caaatcccta
     7861 aatcatccgt ttgaagaacg agactagcat acatgcgtcc gataaatcct gttgctacca
     7921 ccgcaaaaat cactgtaata gcaagtgaaa tccatgcttc tgctcccccc gcatagccat
     7981 taatcgttcg aaacggcata aagaaggtcg aaataaaggg aatataagaa ccaatcttca
     8041 agaggagatt gtcaccagct gcacctagag ctgtcactcc aaaaaaacca cccataatca
     8101 aaatcatcaa aggcgacaag gctttccctg agtcctcagg acgagaaacc atagatccta
     8161 ggaatgctgc caagactacg tacatgaaaa gactgatcaa aataaagagc aaggtattca
     8221 gtgagatagc atctcccaag tgatccaaaa taccagactg agccaagaat ggcaaatctt
     8281 taaagagcaa aacggcagcc agaccaccta caacatagat cccaatatgc gttaaaatca
     8341 ctagaaacag agccatcatc cgcgcataga aatagtgact tgcccttatg ctagaaaaaa
     8401 cgacttccat aattttggtg cctttttcac tggcaacttc ctgagctgtt acacccgcat
     8461 aggtaatcag aatcatataa agaaagaatc ctaaggcacc tgctgcaatt gtttgaataa
     8521 actttttatt ttccttggct tcatcaatct tttctgtgaa ttgaattgtc tgctctaagc
     8581 gtttttcctg ctcttgagac aaggaagcag ttgaacgatt aagctgattt tgcagttcat
     8641 tgagtgtacc tgtaacctca aatttaattc cattttcaag cgatgtttcg ccatgataaa
     8701 ctgcctttag aacactatct tcttgatcaa tggtcaaata accttttaat ttttcttctt
     8761 taattgcttc tttggcactt gcttcgtctt tatagtcgaa gttaacacca tttacattct
     8821 tcagtccttc tgctacagat ggcactgttg tcactactgc cactttatta tttttagcca
     8881 tagaagaacc ttggagatgc ccaattccta cagagattcc taaaaagagg aacggcgaaa
     8941 tcaccataaa gaagaaactc catgactcga catgtcgaag ataggtttcc ttgattacaa
     9001 cccacatatt tctcatactt ccactcctga ttctagttta aagatttcat cgatagttgg
     9061 cgcttgttgg tcaaatgttg cgatatattg accttgagtc aagattggga agagttccct
     9121 tccagcgctc tcatcctcca aaatcaattt ccaactgcct tgtttggtca agctcacctg
     9181 tttgacatga ggaagatttt ccaattcttc cttgcttcgt tcacttgaaa caaagagacg
     9241 cgttttcccg tattgattgc ggacatcctg aactggtccg tgcaagacca cacggccatc
     9301 tcggatcatc agaatatcgt cacaaagttc ctcaacattg gtcatgacat ggtcagaaaa
     9361 gataatggtt gctccgcgct ctttttcctg aaaaatgact tgtttgagca attctgtatt
     9421 aactgggtcc aatccactaa aaggctcatc caagataatc aggtctggtt catgaatcag
     9481 agtaataatg agctgaatct tctgctgatt tccttttgac agactcttga ttttatctgt
     9541 cagctttcct ttcacttcca acctcttcat ccattgaggg agtttttctt tgacttcttt
     9601 ggcatccatg ccttttagag tcgccaagta gcgaacttgt tcaagaactg tcaatttagg
     9661 catgagactg cgttcttcag gcagataacc aatccgagca taggtctcct gacgaatatc
     9721 ctgaccatcc agaccgattt ctccctgata ttctaggaat ttcaaaatac tatggaaaat
     9781 cgttgttttt ccagcaccat tttttccgac tagtcccaaa atacgacctg gtcgcgcttg
     9841 aaagtcaata ccaaacaaaa cttgcttgga gccaaaactt ttctctagac ttcttacttc
     9901 tagcatcttt cacctccgaa atttcttgca ctcattatac tcctttttga tagcctttac
     9961 aatgtttttg tccattttta gaagactatt gctgtgtaaa atatggcctg gagcactttt
    10021 atactcaatg aaaagcaaag agcaaactag gaagctagcc gtaggctgct caaagtacag
    10081 ctttgaggtt gcagata
//
LOCUS       DAVBHS010000072         9992 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVBHS010000072 DAVBHS010000000
VERSION     DAVBHS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9992
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.072"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(339..908)
                     /locus_tag="VQ651_001258"
                     /pseudo
     CDS             complement(339..908)
                     /locus_tag="VQ651_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            1084..2487
                     /locus_tag="VQ651_001259"
     CDS             1084..2487
                     /locus_tag="VQ651_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HET2754261.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            2602..3240
                     /locus_tag="VQ651_001260"
     CDS             2602..3240
                     /locus_tag="VQ651_001260"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HET2754262.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            3252..3692
                     /locus_tag="VQ651_001261"
     CDS             3252..3692
                     /locus_tag="VQ651_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HET2754263.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            3685..4116
                     /locus_tag="VQ651_001262"
     CDS             3685..4116
                     /locus_tag="VQ651_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2754264.1"
                     /translation="MFNRGILVASHGDFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISNYDSKINLEEIVHNAQNSLFNVKQQLNVEEEENLCL"
     gene            4107..4577
                     /locus_tag="VQ651_001263"
     CDS             4107..4577
                     /locus_tag="VQ651_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2754265.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            4602..5375
                     /locus_tag="VQ651_001264"
     CDS             4602..5375
                     /locus_tag="VQ651_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2754266.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            5372..6172
                     /locus_tag="VQ651_001265"
     CDS             5372..6172
                     /locus_tag="VQ651_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2754267.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGDLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            6231..8525
                     /locus_tag="VQ651_001266"
     CDS             6231..8525
                     /locus_tag="VQ651_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET2754268.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPVNLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            8535..>9992
                     /locus_tag="VQ651_001267"
     CDS             8535..>9992
                     /locus_tag="VQ651_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754269.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLH"
BASE COUNT         3335 a         1355 c         2097 g         3205 t
ORIGIN      
        1 tatatttaga tcatcaaggt cacttaattc ttgaaatata gggtctaggc gacaaagtct
       61 ttaaaatcag aaaaacgcat aatatcaggt gtgcaaaaac ttgatactat gcgttttatt
      121 gtgggaaggt ttactccatt ttctcctgaa attgagtttt tgtccagcct ctgtttttag
      181 aattgctaag aaaataatgt catgtggtga atatttgtaa atcagtcagc agacagaacg
      241 aatactcttc gaaaatctct tcacatcatg tcagcttcgt cttgccgtgt ccatggggga
      301 cttgacttga tcagttttca aatctgtact ttgagcaagc tgagactagc ttcataaaca
      361 tattggtcat caatacctct tgctgaacaa aatgctaaat cgatattaaa atgatctaat
      421 aaagaatttt ccttatcata gttgaccacg gaacaggatt gatgtttgac ctcgccagat
      481 gtgataaaga ttttggagct atctttaaca gtttcagata gggtttgtgc agtatgtaaa
      541 ccatttgtaa aaataatcaa attatcaagt tcagaaagat agggacaaag ttcgtagaca
      601 gtagtactag aatctagata gatacacata ccagaccgaa taaagtcttt agcgagacta
      661 gcgattagtc ttttttgcct agtactttct ccttcccgta tttgatgaga aagttcaatt
      721 gtgttcatag aggacagggt cacgtatccg tgctttcttt tgataagacc ttgattttct
      781 aagaaaatta aatcacgacg taaggtactt gtgctggaga aagtgatttc tgccagctct
      841 tttacggcaa ttcttttttt ctttttaata atttcaatca attcaagtac acgttcatct
      901 tttatcataa gctcctccta atttatcatt tcaactatat tatagcacaa attggaggaa
      961 tttgaattat ttttatgaat attgggttaa catttgaaca ttattcaagt aagcgttcac
     1021 atattgaaaa aataaaacgt ggggattata ataaagttaa tcaaggacga agagagaaga
     1081 aaaatggaag cggttttagc aatagattta ggtgcgactt ctggaagagc aatcgttggt
     1141 tacctttctg aaaataaact agtaatggaa gaaataaatc gcttttctaa tctacctatt
     1201 agagtaaaag ggcatttatc ttgggatatt gactttctac tagctaaaat tcttgaaagt
     1261 atccgcttgg ctaatactag ttacaagatt ttatctatcg gtattgacac atggggagtt
     1321 gattttggac tgattgataa tgaaggtaag ctgttatcac aacctgttca ttatcgtgat
     1381 gaaagaacaa agggagtgtt aaaggaaata tctgaaatga ctgaattaga aaaactgtat
     1441 tcagagacag gaaatcagat tatggagata aataccttgt ttcaactctt taaggcacgt
     1501 caagaatctc ctgactcttt ctataagacc aataagattc ttttaatgcc agatttgttt
     1561 aattatctct tgacaggtaa gtttgctaca gaaaaaagca ttgcttcaac aactcaatta
     1621 tttgatccta ggagtcaaaa ttggaatcag aatatcttaa aactatttga attggattca
     1681 tctttacttc ctgaaattgt ttcagaggga aatgttcttg gaaggataaa aaaggagtat
     1741 ggtttaggcg atattcctgt tgtgaatgtt tgtagtcatg atacagcaag tgcgattgtc
     1801 tcagtaccta agacagaagg tagtttattt atttcatcag gtacttggtc tttggttgga
     1861 gtggaactta cttcaccgat tcttactacc gaatccttca gttatggatt tacaaatgaa
     1921 gtcggtaaag atggagtgat tacatttctg aagaattgta cagggttgtg gatcatagag
     1981 gaactaagac gttcatttga acgaagaggg aaagcctatt cttttgatga tattaggaca
     2041 atggtggaga aagaaaaaga aaatcttcct ctgattgata ctgaatcaac tgaatttgca
     2101 acagaatctg atatgcacaa gactttgaca gaatatctag cttatcatca tgaaactaga
     2161 gagtggacag atggacaact atttaagatt gtttatgaaa gcctagctga aacgtatagg
     2221 aaagcgatag agttattaga agaactaact cataaggttt ataagaggat atatgtgatt
     2281 ggaggaggtg ctagagccag ttattttaac caaatgattg ctgatagaac tggtaaagag
     2341 gttcttacag gtttgactga ggctacagct gtggggaata ttgttgtgca gctcatagct
     2401 atgggacaat taaaagggat ggaagaggct caccatgtta ttgaggagtt tctacaatta
     2461 gagagttatt actcccaaaa gaattaaaaa gattgagagt ttgtaaattt gcctccctcc
     2521 cccttcttag cttttgtgca ggaagggggg ataattggtg aattgaaaaa tatttagtgt
     2581 tttgatatga ggaggacaag gatgtcagat gtaaaacaag aattaattaa atatggtaag
     2641 aagctagtag aaacagattt gacgaaagga acaggtggga atctcagcgt tttcgatcgt
     2701 gaaaaacaat tgatggcaat taccccgtcg ggtattgatt tctttgaaat caaagaatcc
     2761 gatattgtag tgatggatat taatggaaat gttgtagagg gagaacgctt gccatctagc
     2821 gaatggtata tgcatttgat tcaatatcaa actcgtgatg atatcgatgc aattatccat
     2881 gctcatacaa cttatgcaac agtattagct tgtctcagag aaccacttcc agcgagtcat
     2941 tatatgattg cagtggcagg gaaagatgtt cgggtagctg agtatgcaac atatggcacg
     3001 aaagaattgg ctgtgaatgc agctaaagca atggaaggtc gtagagcagt tttactagcg
     3061 aatcatggaa ttttagcagg tgcacaaaat ttattgaatg catttaatat tgttgaagaa
     3121 gttgaatatt gtgcaaaaat ttattgttta gctaagaatt ttggagagcc agtagttctt
     3181 cctgatgagg agatggaatt gatggcagaa aaatttaaaa catacggtca gagaaaatag
     3241 ggaggatatt aatgttaaaa catataccga aaaatatttc tccagattta ttgaagactt
     3301 taatggaaat gggacatgga gatgaaatag tattagctga cgcgaattat ccttctgcct
     3361 catgtgcaaa taagctaatt cgttgtgatg gcgtaaatat tccagaatta ttagattcca
     3421 ttctgtattt aatgccatta gatagttacg tcgatagttc aattcagttt atgaacgttg
     3481 tttcgggtga tgatattcct aagatatggg gtacctatag acagatgatt gaaggtcatg
     3541 gtacagatct taaaacgatt acttatctta gaagagaaga cttttatgaa cgtagtaaga
     3601 aagcttatgc tattgttgct acaggagaaa cttcacttta tgctaatatt atccttaaga
     3661 aaggagtagt tgttgaaaga gaaaatgttt aatagaggaa ttttagttgc cagtcatggt
     3721 gattttgcta gcggagctct catgaccgca gaaatgtttg ttggtgagac aacaaatgat
     3781 agagttagga cattaggttt gatgcctgga gagaatattg tagagtttga gcattatttt
     3841 aaaaatcaag tggatgaact gttagactca aatcaagagg ttatcgtttt gactgacttg
     3901 attggaggaa gtcctaataa tgtggctttg tcacggtttt taaatttgga ttcagttgat
     3961 attgtaacag ggtttaatat tcctctccta gtggaattaa tatcaaatta tgattcaaaa
     4021 atcaatttag aagaaattgt tcacaatgct caaaatagtt tgtttaatgt taaacaacaa
     4081 cttaacgtag aggaggaaga aaatttatgt ctatagagtt tgttcgtatt gatgaccgtc
     4141 tggtacatgg tcaagttgtc actacgtggc taaaaaagta tgatattgag caagttatca
     4201 ttgttaatga tcgcatctca gaagataaaa cacgacaatc tattttaaag atttctgcac
     4261 cagtaggttt aaaaattgtt ttctttagtg taaaacggtt tgtggaagtt ttaaactctg
     4321 tgccaataaa aaagagaaca atgctgatat atacaaatcc aaaagatgtg tatgattcta
     4381 ttgaaggaaa tttaaaattg gagtacctca atgtaggaca gatgagtaaa acggaggaaa
     4441 atgaaaaggt aacgggaggt gtagctctag gtgaagaaga caaatattat tttaagaaaa
     4501 tagttgataa gggaacgaga gttgaaattc aaatggttcc taatgataaa gttacaatgt
     4561 tggaaaaatt tttataaaaa taatttaagg aggtacagta tatgctattc acacaagcat
     4621 tactggtgac attagttggg attattgcca ctattgacta taatggaccg ttatttatga
     4681 ttcaccgtcc gttagttaca agtgcaatgg ttggcttagt cttaggagat ttcacccaag
     4741 gtgttcttat tggttcagct cttgaattaa cttggctcgg tgtaacaggt attggaggtt
     4801 atactccacc agatactatt tcaggtgcga ttattggtac tgcatttggt attttatctg
     4861 gtcaaggaga aactgctggt atcgctatag cagttccaat tgcagttgct acccaacagt
     4921 tggatgttct tgcaaaaact ttagatgttt attttgtgaa aaaagctgat aatgatgcta
     4981 aaaacggaga ttattcaaag atcggttttt atcattattc aagtttggtt ttaatcacgt
     5041 tatttaaaat tgtaccaatt ttcctagcta ttatgcttgg aggggaatat gtggcagact
     5101 tgtttgctaa ggttccacca atcgttatgc agggacttaa ctctgcaggt gctttactac
     5161 cttcaattgg ttttggtatg cttttaaata tgatgctcaa gaaaaatatg tgggtattct
     5221 tgttgattgg attcatttgt tctgtgtatg gaggaatgtc aaccattggg atctcactag
     5281 ttggtattgc ggtagcatac ttctacgata tgattggaag taaaccacaa gaaacaactt
     5341 caagtagtga tgttgaggag gatcttgatc tatgatgaat aataaagtaa ctaaagttga
     5401 acttaaaaaa gttttcaaac gaagttttat gtatggttct tcatggaact atgagagaat
     5461 gcagaaccta ggttttctat atacaattct tccagtattg aaaaaactat acccagacaa
     5521 agattcagct tctcctgcaa tgaaacgtca ccttgagttt ttcaatactc atcaaacagc
     5581 ggcaccattt attcttggag ttacttccgc tatggaagaa caagaaggaa atgaaggtgc
     5641 agcttcaatt actggtatta aagttggctt gatggggcca ctggctggtc taggagatag
     5701 tttgttctgg ctgacactag ttcctatctg ttttagtatt ggtgcgtctt attctaaaga
     5761 cggcggtgct ttaggtatct ttatcgcctt aatattgttt aatattatta atattcctgt
     5821 taaatatttc ggtttgaaat atgggtatac taagggttct agtcttatcc aagaaaataa
     5881 tacaaaagga acattgaatc gcgttacgag tatggcgaca gcattagggc tagtactagt
     5941 gggtgatttg attccatcaa tggttggtat taattttgga ttagaattta agcaggggga
     6001 acttgttatt tctgttcaag aaatgattac aaaattaatt ccaggattta tccctatggc
     6061 tttgacttta ttaatgtgta aattaattag aaaaggaaag aatccggttg tactaatctt
     6121 tagtgttatg gctattggag ttatcctagt tgttttagga attttgaagt agtagaaagt
     6181 gtggaggtgg tatttgggat atcacctcca ttttggaaga gaggtaaaga gtgaaattat
     6241 ggtataagaa agctgcctca aattggaatg aagccttgcc gattgggaac ggtcatttag
     6301 gtggtatgat ttatggttca gctacaaaag aatgtattca actaaacgat gagactattt
     6361 ggtatagagg aaagtcagat agaaataatc cagactcact attgcatctt aaaaaaattc
     6421 gggaatatct tttagatgga gaaattcaga aagccgaaga attgataaag ttaacaatgt
     6481 ttgctacccc aagagatcaa agccactatg aattacttgg ggaactttac attgagcata
     6541 tagatattca gtcttgtgct ctttcattgt atgaaagaga gctagattta gatacagcta
     6601 tttctaatgt tgtgtttgag cctaatagtt gtaatttaca aataaaaaga gaatatttta
     6661 cgagttttaa taagaatatt ttatgttgcc gtatagtgtc atcagttcaa aacacattaa
     6721 atttaaacat taatttgggt agaaataaac ggtttaatga cgaagtatct aaactggatt
     6781 caagtacaat tttaatgtcg gcctctgctg gaggtagaaa aggtgttcag tttaaagtag
     6841 tatgtcattc taaagttacg gatggtgaag taagtgtatt gggagagaca atagttattc
     6901 ggaatgctac agaggtattt ctttatctca aatcaatgac ggattattgg ggaaatatag
     6961 atatttcttc tcttcaggga gaatttagta gtattgatta ctttacagaa aaagatgaac
     7021 atgtaaaaaa atatcaggag caatttaata gagttgattt taaactagac tatagtaaag
     7081 attgtcttag cattccaacg aatctacttc ttgaaaacac taaaaagtat agtaactact
     7141 tgactaactt gttatttcat tatggaagat atctgttaat atcgtctagt caaccgaatg
     7201 gtttacctgt caatcttcaa ggaatatggt gtgatgaatt aaatccaatt tggggttcta
     7261 aatatacgat taatattaat actcaaatga attattggat ggtaggtcca tgtgatttac
     7321 cagaagtaga atatccatta tttgatatgc tcgaaagaat gagagaaccg ggaagactaa
     7381 ccgctaagaa aatgtatgga gctagaggtt ttacagcaca tcataatacg gatggttttg
     7441 gcgatacggc tccccaatct catgccatgg gggctgcaat ttgggtatta actattccat
     7501 ggttatgtac tcatatttgg gaacactatt tatatttcca agatgagcgt attcttacgg
     7561 aacattttga aatgataaaa gaagcatttc ttttctttga agattattta tttgaggtgg
     7621 atggctactt gatgacaggt ccaagtgtct caccggaaaa taaatatcgc ttaaaaaatg
     7681 gtattgaagg aaatgcttgt ctatcatcta caattgataa tcaaattcta agatattttt
     7741 gtgattcatg cattggcatt gcaaaacaat taggagacaa ttcggatttt attagtcgtg
     7801 tgaaggagtt aaaaaagaaa ctacctaaaa caaaaatagg tagtaatggg caaatccaag
     7861 aatggttaga agattatgaa gaagtagagc ctgggcatag acacatttca cctctatttg
     7921 ggctttatcc ttataatgag attgatattc ataaaactcc ggaattagca gaagcagcta
     7981 aaatcactat caataggaga ttatcaaacg ctaacttttt atcttcacag gagagggagc
     8041 aagcgattaa taattggtta gtaagtggtt tgcatgctag tacacaaaca ggttggagtg
     8101 ctgcatggct gattcatttt tttgcgagac tatatcaagg tgaacctgct tataaccaga
     8161 ttaatggttt gttaaataat gcgactcttg gcaatttatt tcttgaccat ccaccatttc
     8221 aaattgatgg taatttaggt ttggtgagtg gaatttgtga attattagta cagagccatc
     8281 ataattggtt atcactaatt ccagctttac cttctgcttg gtcagaagga gaagtgaaag
     8341 gtttcagagt aagaggagga tataaggtat cgtttgcttg gaaaaatggg gatataacat
     8401 tcctaaaatt ggaaggagga aacaaagatc aaaaagtaag agtaagaata tatggcaaaa
     8461 atactgatgt acaaaatatt gaattggtat ttaattcaga aaaaattatt gagttaaatt
     8521 tttaggtata agtcatgaat aaagaaaaaa taaaaagaaa attaatcaca atactgtttg
     8581 tatgtattgg gatgttatgt tttggattgt tagcaggagt taaggctgat aatcgtgttc
     8641 aaatgagaac gacgattaat aatgaatcgc cattgttgct ttctccgttg tatggcaatg
     8701 ataatggtaa cggattatgg tgggggaaca cattgaaggg agcatgggaa gctattcctg
     8761 aagatgtaaa accatatgca gcgattgaac ttcatcctgc aaaagtctgt aaaccaacaa
     8821 gttgtattcc acgagatacg aaagaattga gagaatggta tgtcaagatg ttggaggaag
     8881 ctcaaagtct aaacattcca gttttcttgg ttattatgtc ggctggagag cgtaatacag
     8941 ttcctccaga gtggttagat gaacaattcc aaaagtatag tgtgttaaaa ggtgttttaa
     9001 atattgagaa ttattggatt tacaataacc agttagctcc gcatagtgct aaatatttgg
     9061 aagtttgtgc caaatatgga gcgcatttta tctggcatga tcatgaaaaa tggttctggg
     9121 aaactattat gaatgatccg acattctttg aagcgagtca aaaatatcat aaaaatttgg
     9181 tgttggcaac taaaaatacg ccaataagag atgatgcggg tacagattct atcgttagtg
     9241 gattttggtt gagtggctta tgtgataact ggggctcatc aacagataca tggaaatggt
     9301 gggaaaaaca ttatacaaac acatttgaaa ctggaagagc tagggatatg agatcctatg
     9361 catcggaacc agaatcaatg attgctatgg aaatgatgaa tgtatatact gggggaggca
     9421 cagtttataa ttttgaatgt gccgcgtata catttatgac aaatgatgta ccaactccag
     9481 catttactaa aggtattatt cctttcttta gacatgctat acaaaatcca gctccaagta
     9541 aggaagaagt tgtaaataga acaaaagctg tattttggaa tggagaaggt aggattagtt
     9601 cattaaacgg attttatcaa ggactttatt cgaatgatga aacaatgcct ttatataata
     9661 atgggagata tcatattctt cctgtaatac atgagaaaat tgataaggaa aagatttcat
     9721 ctatattccc taatgcaaaa attttgacta aaaatagtga ggaattgtct agtaaagtca
     9781 actatttaaa ctcgctttat ccaaaacttt atgaaggaga tgggtatgct cagcgtgtag
     9841 gtaattcctg gtatatttat aatagtaatg ctaatatcaa taaaaatcag caagtaatgt
     9901 tgcctatgta tactaataat acaaagtcgt tatcgttaga tttgacgcca catacttacg
     9961 ctgttgttaa agaaaatcca aataatttac at
//
LOCUS       DAVBHS010000073         9981 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVBHS010000073 DAVBHS010000000
VERSION     DAVBHS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9981
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.073"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            398..1091
                     /locus_tag="VQ651_001268"
                     /pseudo
     CDS             398..1091
                     /locus_tag="VQ651_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1147..1419
                     /locus_tag="VQ651_001269"
     CDS             1147..1419
                     /locus_tag="VQ651_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HET2754270.1"
                     /translation="MFLLFSALVGMLSQLKEVPESVFLQETVEENHLVNVYSVLEVIS
                     TLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK"
     gene            1733..1864
                     /locus_tag="VQ651_001270"
     CDS             1733..1864
                     /locus_tag="VQ651_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HET2754271.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            1885..3435
                     /gene="dltA"
                     /locus_tag="VQ651_001271"
     CDS             1885..3435
                     /gene="dltA"
                     /locus_tag="VQ651_001271"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HET2754272.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFVQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMIMDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGVTHFYFDG
                     EELTVKTAQKLRKRFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            3432..4676
                     /gene="dltB"
                     /locus_tag="VQ651_001272"
     CDS             3432..4676
                     /gene="dltB"
                     /locus_tag="VQ651_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HET2754273.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            4690..4929
                     /gene="dltC"
                     /locus_tag="VQ651_001273"
     CDS             4690..4929
                     /gene="dltC"
                     /locus_tag="VQ651_001273"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HET2754274.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            4922..6190
                     /gene="dltD"
                     /locus_tag="VQ651_001274"
     CDS             4922..6190
                     /gene="dltD"
                     /locus_tag="VQ651_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HET2754275.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            6345..6785
                     /gene="adcR"
                     /locus_tag="VQ651_001275"
     CDS             6345..6785
                     /gene="adcR"
                     /locus_tag="VQ651_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HET2754276.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            6785..7489
                     /locus_tag="VQ651_001276"
     CDS             6785..7489
                     /locus_tag="VQ651_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET2754277.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNTHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            7482..8288
                     /locus_tag="VQ651_001277"
     CDS             7482..8288
                     /locus_tag="VQ651_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET2754278.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGRSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            8298..9803
                     /gene="adcA"
                     /locus_tag="VQ651_001278"
     CDS             8298..9803
                     /gene="adcA"
                     /locus_tag="VQ651_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HET2754279.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKVTFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
BASE COUNT         2859 a         1882 c         2134 g         3106 t
ORIGIN      
        1 ctactcgatt aactcccctg aaatttgttt taattatgca aatttcacgt atttttgatg
       61 ctgagacgac gatcctggga acttttcaga tatttttttg actatctaaa atctatcatt
      121 agaaaagctt agagcgccaa aggatttgag cgtttttctg atttttaaga ctttttccca
      181 gtctcttttt cgattgaaga tgtaattatt ctactaacta acttcttagt actagccaac
      241 aacgataatc ataattcctc ctaaaattag gaataataaa ggcaatagtt tttgtttttt
      301 catgtaaaaa acctcacttt tgttttctgc tattttatgc taaaatatta aaaatcaaat
      361 ttaattccaa agtttgtaac taaaggggga gcgctacatg tctaattcat ttgtcaagtt
      421 gttagtctct caattatttg caaatttagc agatattttc tttagagtaa caatcattgc
      481 taacatatac attatttcaa aatcagtaat tgccacatca ctagttccta tcttaatagg
      541 aatatcctct tttgttgcga gtcttttagt tccgttggtt actaaaaggt tagcgctaaa
      601 tagggtttta tctttatctc aatttggaaa gactatatta ttggcgatac tggtaggaat
      661 gtttaccgta atgcaatccg tagcgccttt ggtgacctat ctatttgttg ttgcaatttc
      721 catactagat ggttttgcag cacccgtttc ctatgctatt gtgccacgct atgcgaccga
      781 tttgggtaag gctaattcag ccttatcaat gactggtgaa gctgttcaat tgataggttg
      841 gggattaggt ggactcttgt ttgcaacaat tggtctgtta cctaccacgt ttatcatttt
      901 agtcttgtat atcatttcta gctttctgat gttatttctt cctaacgctg aagtggaggt
      961 gttagagtca gaaactaatc ttgaaatttt gctcaaaggt tggaagttag ttgctagaaa
     1021 tcctagatta agactttttg tatcagcaaa tttattggaa attttttcaa atacgatttg
     1081 ggtttcttcc attttgtagc tatggcaata gtgacactga ctattctata ttttccaaac
     1141 gcacagatgt ttttattatt ttcagcttta gttggtatgt tatcgcaact aaaagaagtt
     1201 cctgaaagtg tatttcttca ggaaacagta gaggaaaatc atttagttaa tgtctattcc
     1261 gttcttgaag tgatttctac attagcattt tcagtatttg ttttgctaat gagctatatt
     1321 actgagagtt ttggtattag catcagtttt tggctatcag ccatttgtct gatgatagaa
     1381 gctattttaa tttatatcag acgagattat tttaaatgag agagtggtta aaaagtcttt
     1441 aaaaatcaga aaagcgcata gtatcaggtg ttgaaaaaac ttgatattgt gtgttttatt
     1501 atgggaagat ttacttcatt gtctcctgaa atggagtttt gaagcaggat cactctattt
     1561 gaaaatatac atagaaaaac tgtataatcc ttaaaataag ctgaaatcta ttggaaacat
     1621 acggtaaaca attgaaaagg tactatataa agtggtacaa tggtagaaaa ataagctagt
     1681 aagaatcact cttattttag cgaaaaatta cagaaatgaa tggtttttct tgatgaaaca
     1741 gagaaaagaa ttgtacctct ttcttggtcg gacagccttg tattttctta tctttctagg
     1801 gctgctttac ttctttagct atcttggtca gggtcaagga agctttatct ataatgaatt
     1861 ttaacttgaa ggagaatccc tattgtgtca aataaaccaa tagcagatat gattgaaacc
     1921 attgagcatt ttgctcagac acagcctagc tatcctgtct ataatgtttt ggggcaggaa
     1981 cacacttatg gcgatttaaa ggctgattcg gatagtttgg ctgcagtcat tgaccaacta
     2041 ggcttgcctg agaagtctcc tgtggttgtt tttggtggcc aagaatatga aatgttggca
     2101 acctttgtag cgctgactaa gtcaggtcat gcctacattc caattgatag ccattcggcc
     2161 ttggagcgag tttcagctat tttagaagta gcagagccaa gcttgattat tgccatttca
     2221 gcctttccct tggagcaggt ttctacacca atgataaatc tagctcaggt tcaagaagcc
     2281 tttgttcaag ggaataacta tgaaatcacg catccagtca agggagatga taattactac
     2341 attatcttta cttctggtac gactggtaag cctaagggag tgcagatttc acatgataat
     2401 ctcctcagct ttacaaactg gatgattatg gataaggaat ttgcgacacc gagtcgtccg
     2461 caaatgctgg cacagccacc ttattctttt gacttgtctg tcatgtattg ggcaccgacc
     2521 ttggcacttg gtggtacgct tttcactctt ccttcagtca tcactcagga ctttaagcaa
     2581 ctctttgcgg ctatcttttc attgccaatc gctatctgga catcaacacc atcctttgca
     2641 gatatggcca tgttgtctga atacttcaac agtgagaaaa tgcctggagt cacgcatttc
     2701 tactttgatg gtgaagaatt gacggtcaaa acagctcaaa aactgcgcaa gcgtttccca
     2761 aatgcccgta tcatcaatgc ttacggccca acagaagcga cagtagctct gtcagcagtt
     2821 gccgtgacag acgagatgtt agcgactctc aaacgcctac caatcggcta taccaaggct
     2881 gattctccaa cctttatcat tgacgaggaa ggaaataaac tgccaaatgg cgaacaggga
     2941 gaaatcattg tttctgggcc agctgtttca aaaggttata tgaacaatcc tgaaaaaaca
     3001 gcagaagcct tctttgagtt tgaaggtctg ccagcctatc acacaggcga tgtgggaacc
     3061 atgacagatg agggcttgct tctctacggc ggacgcatgg acttccagat taagtttaac
     3121 ggttaccgca ttgagttaga agatgtctct caaaacctca acaagtctcg ctttatcgaa
     3181 tctgctgtcg cagtaccgcg ctataacaag gaccacaagg tacaaaatct attggcttat
     3241 gtcatcttaa aagacggtgt tcgtgagcag tttgagcgag atatcgatat taccaaggcc
     3301 atcaaggaag acctgacaga catcatgatg tcctatatga tgccatctaa attcctttac
     3361 cgagacagtt tgccactgac tccaaatgga aagattgaca tcaaaggatt gattaacgag
     3421 gtgaataaga gatgatggag ttttttcaac agcttcctca tttagagcca tatggcaatc
     3481 ctcagtattt tgtttatgtg attgctgcaa ccttgcccat ctttataggt ctctttttca
     3541 agaaacgctt tgcctggtat gaagtgttgg taagtctctt ctttattgtc accatgttgg
     3601 tgggtggaaa gaccaatcaa ctagctgcct tgggtattta cctttgctgg gaaatattgc
     3661 tcctgctttt ctacaagcat tatcgaaaaa gcaaggatgg caagtgggtc ttctacttag
     3721 ttagttttct gtccctactt ccgattatct ttgtcaaggt gcaaccagct atcaatggaa
     3781 cgcagtcttt gcttgggttc ttgggaattt cttatcttac ctttcgttcg gttgggatta
     3841 tcatcgagct gagagatgga gtgattaagg attttaccct ctgggaattc ctccgtttcc
     3901 ttctcttcat gccaactttc tcgagtggtc caatcgatcg ctttaagcga tttaatgaaa
     3961 attatcaggc tattcctgag cgagatgagt tgatggatat gctggatgaa tctgtccgct
     4021 atatcatgtg gggctttttg tataagttta tcctagctca tgttttagga gagaccttac
     4081 tacctcctct gaagaattta gccttgcagt caggtggctt ctttaatctc tatgccttgg
     4141 cagttatgta tacttttggt ctggagctct tctttgactt tgcaggttat tctatgtttg
     4201 ctttggccat ctcaaacttg atgggaatcc gtagccctat caactttaac aagccctttt
     4261 tatcaaggga tttaaaggag ttttggaatc gctggcatat gagtctgtcc ttctggttcc
     4321 gtgactttgt ctttatgcga atggtgatgg tgttaaccag aaagaaagtc tttaaaaatc
     4381 gtaatgtaac ctcaagcatg gcctacattg taaatatgct gattatggga ttttggcatg
     4441 gtgtgacctg gtactatatc gcctatggac tctttcatgg actaggcttg gtcatcaatg
     4501 atgcctgggt tcgcaagaaa aaaacactca ataaggaacg gaaaaaagca gggaaggctg
     4561 ccctacctga gaatcgctgg attcagttgc ttggcatggt tgtcactttc catgttgtca
     4621 tgttgtcatt cttaatcttt tctggattct tgaataatct atggtttaaa aaataaaagg
     4681 aaataaaata tggatatcaa atcagaagtt atcgaaatta ttgatgagtt gtttatggaa
     4741 gatgtttctg acatgatgga tgaagatctt tttgatgcag gtgtcttgga tagtatggga
     4801 acggttgagt tgattgtgga gattgagaac cgttttgaca ttcgtgtccc tgtaacagag
     4861 tttggtcgtg acgactggaa tacagctaat aaaatcatag ctggtattgt ggagctacaa
     4921 aatgcttaaa cgcttatgga tgatcttcgg accggtcttg atcgctggtt tgttggtttt
     4981 tctgctcatt ttcttttatc ctactgagat gcatcataat ctaggagctg aaaagcgttc
     5041 agcagtggct actactatcg atagttttaa ggagcgaagt caaaaagtca gagcactatc
     5101 tgatccaaat gtgcgttttg ttcccttctt tggctctagt gaatggcttc gttttgacgg
     5161 tgctcatcct gcggtattag ctgagaaata caatcgttcc taccgtcctt atcttttagg
     5221 acagggggga gctgcatcgc ttaaccaata ttttggaatg caacagatgt taccacagct
     5281 ggagaataaa caagttgtgt atgttatctc acctcagtgg ttcagtaaaa atggctatga
     5341 tccagcagcc ttccagcagt attttaatgg agaccagttg actagttttc tgaaacatca
     5401 atctggggat caggctagtc aatatgcagc gactcgctta ctgcaacagt tcccaaacgt
     5461 agctatgaag gacctggttc agaagttggc aagtaaagaa gaattgtcga cagcagacaa
     5521 tgaaatgatt gaattattgg ctcgttttaa tgaacgccaa gcttcctttt ttggtcagtt
     5581 ttcggttaga ggctatgtta actacgataa gcatgtagct aagtatttaa aaatcttgcc
     5641 agaccagttt tcttatcagg caatagaaga tgttgtcaaa gcagatgctg aaaaaaatac
     5701 ttccaataat gagatgggaa tggaaaatta tttctataat gagcagatca agaaggattt
     5761 gaagaaatta aaggattctc agaaaagctt tacctatctc aagtcgccag agtataatga
     5821 cttgcagttg gttttaacac agttttctaa atctaaggta aacccgattt ttatcattcc
     5881 acctgttaat aaaaaatgga tggactatgc tggtctacga gaggatatgt accaacaaac
     5941 ggtgcagaag attcgctacc agttagaaag tcaaggtttt accaatatag cagatttttc
     6001 taaggacggc ggggagcctt tctttatgaa ggacaccatt caccttggtt ggttgggttg
     6061 gttggctttt gacaaggcag ttgatccttt cctatccaat cccacaccag ctccgactta
     6121 ccatctgaat gagcgctttt tcagcaaaga ttgggcgact tatgatggag atgtcaaaga
     6181 atttcaatag ataatataat atagaagagt tcaagaatga aactagtttt cacgtttttt
     6241 cttgactctt tttttgcttg tgatataatt aactggtaaa caaaatgtca aagaatagta
     6301 tttttctctt aaaaaagaga agtctaaaag gaaaggagtc agatatgaga cagctagcaa
     6361 aggatatcaa tgcttttttg aatgaggtga ttttgcaggc ggaaaatcag catgaaatcc
     6421 taataggtca ttgcactagc gaggtggccc tgaccaatac tcaggagcat atccttatgc
     6481 tcttgtcaga ggaatcttta acaaattcag aattggcccg tcgtctcaat gtcagtcagg
     6541 cggcagttac caaggccatt aagtctttgg tcaaggaagg gatgttggaa acatctaaag
     6601 attctaaaga tgcgcgtgtg attttttatc agttgactga cttggctcgt ccaatcgctg
     6661 aggagcacca ccatcaccat gagcatacac ttttaaccta tgaacaagtg gcgactcagt
     6721 ttactccaaa tgaacaaaaa gtgattcagc ggtttttgac tgctttagta ggagaaatca
     6781 aataatgaga tatattacgg tagaggattt gtccttctat tatgataagg aacctgttct
     6841 tgaacatatc aattattgtg ttgatagtgg ggaatttgtt accttgactg gggaaaatgg
     6901 agcggctaag acgacgctca tcaaggccag tcttggaatt ctgcaaccac gcattggaaa
     6961 ggtggctatt tcaaagacaa atacgcaagg taagaaattg agaatagcct atcttcctca
     7021 acaaattgcc agttttaatg ctggttttcc aagtacggtc tatgaatttg tcaagtcggg
     7081 tcgctatccg agaaaaggct ggttccgtcg tttgaatact catgatgagg agcatatcaa
     7141 ggctagtctg gactcagttg gcatgtggga acatcgagac aaacgcttgg ggtctctatc
     7201 tgggggacaa aagcagcgag cggtaattgc gcgtatgttt gcttctgacc ctgatgtgtt
     7261 tatcctagac gagccgacaa cggggatgga tgcaggaagt aaaaatgaat tttacgaact
     7321 catgcaccac agcgcccatc atcatggcaa ggctgttttg atgattaccc atgaccctga
     7381 agaagttaag gattatgcgg atcgcaatat tcatctagtc cgtaaccaag actcgccatg
     7441 gcgttgtttc aacgttcatg agaatgggca ggaggtgggc catgcttagt ttattatctt
     7501 acgactttat acaacgcgcc tttttggcgg ttattgctat gagtcttttc tcaccggtat
     7561 tgggaacctt cctcatcttg cgtcgtcaga gtttgatgag tgataccctt agccacgtct
     7621 cactttcagg tgtagccttt ggtctggttt tggggatttc tccaactgtt tctactattg
     7681 ccattgtctt gattgcggcg gtctttctgg agtatctccg tacggtttac aagagcttta
     7741 tggaaatcgg gacagctatc ctcatgtcaa caggtttggc tgtttctctg attgtcatga
     7801 gcaagggtag aagctcgagt tcaatgagtt tggaccaata tctctttggt tcgatcgtga
     7861 ctatcagtga agaacaggtc atttccctct ttgtcattgc ggcggttgtt ttgattttga
     7921 cctttctctt tcttcgtcct atgtatatct taacttttga cgaagatacg gcctttgtgg
     7981 atggcttgcc agttcgtacc atgtccattc tttttaacat ggtgacaggg gtggctattg
     8041 cccttatgat tcctgcagca ggagctcttc tggtatcgac cattatggtc ttgccagcta
     8101 gtattgccct gcgtctgggg aaaaacttta aatcggttat gctgcttgcc agtgcgattg
     8161 gctttttggg aatggtagca ggactttaca tttcctacta tgcagaaaca cctgcaagtg
     8221 caagtattac cattattttt gtaactgtct ttatactaat cagtttagta agacgtttta
     8281 tcaaatagga gacgaacgtg aaaaaaatta gcttattact agccagtcta tgtgccttgt
     8341 ttttagtggc ttgttccaat caaaaacagg cagatggtaa actcaatatc gtgacaacct
     8401 tttaccctgt ctatgaattt accaagcaag tcgcaggaga tacggctaat gtagaactcc
     8461 taatcggtgc tgggacagaa cctcatgaat acgaaccatc tgccaaggca gttgccaaaa
     8521 tccaagatgc agataccttc gtttatgaaa atgaaaacat ggaaacatgg gtacctaaat
     8581 tgctagatac cttggataag aaaaaagtga aaaccatcaa ggcgacaggc gatatgttgc
     8641 tcttgccagg tggcgaggaa gaagagggag accatgacca tggagaagaa ggtcatcacc
     8701 atgagtttga cccccatgtt tggttatcac cagttcgtgc cattaaacta gtagagcaca
     8761 tccgcgacag cttgtcagca gattatcctg ataaaaaagt gacctttgag aagaatgcag
     8821 ctgcctatat cgaaaaattg caagccttgg ataaggctta cgcagaaggc ttgtctcaag
     8881 caaaacaaaa gagctttgtg actcaacacg cagcctttaa ctatcttgcc ttggactatg
     8941 gactcaaaca agtcgcaatc tcaggacttt ctccagatgc agagccatca gctgctcgct
     9001 tggcagaatt gacagagtat gtcaagaaaa ataaaatcgc ctatatctat tttgaagaaa
     9061 atgcctcaca agcccttgct aacacacttt caaaagaagc aggtgtcaaa actgatgtcc
     9121 tcaatccttt agaaagtctg acagaagagg acaccaaggc tggagaaaac tacatttccg
     9181 tgatggagaa aaacctcaag gctttgaaac aaacaacaga ccaagaaggc ccagcaatcg
     9241 aacctgaaaa ggcagaggat accaagacag tccaaaatgg ttacttcgag gatgcagctg
     9301 tcaaggaccg caccttgagt gactatgcag gtaactggca atcagtttat cctttccttg
     9361 aagacggcac gtttgaccaa gtctttgact acaaggctaa gttgactggt aagatgaccc
     9421 aggctgagta caaggcttac tatacaaaag gctatcagac agatgtgact aagattaaca
     9481 ttactgataa tactatggaa tttgttcaag gtggacaaag caagaaatac acttacaagt
     9541 atgtcggtaa gaaaattttg acttacaaga aaggcaatcg tggcgtgcgt ttcctctttg
     9601 aagccacaga tgctgacgct ggacaattca agtatgttca gtttagtgat cacaatgttg
     9661 ccccagttaa ggcagaacat ttccatatct tctttggagg cacaagccaa gaagccctct
     9721 ttgaagaaat ggacaactgg ccaacctact acccagataa cctatctggc caagaaatcg
     9781 cccaagaaat gttggcgcat tgatgagaaa tcgactagtt cataagagct agtcggtttt
     9841 ttgatactag atggctaact tcattataga cctttcagaa ctttaaacaa taaataaaac
     9901 tcttgaaata tctgggctta tatgctagaa tgtagttagt gtataaggtt cattagaaca
     9961 ccaaagcccc taactagtgc c
//
LOCUS       DAVBHS010000074         9978 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVBHS010000074 DAVBHS010000000
VERSION     DAVBHS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9978
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.074"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(36..1739)
                     /gene="ilvD"
                     /locus_tag="VQ651_001279"
     CDS             complement(36..1739)
                     /gene="ilvD"
                     /locus_tag="VQ651_001279"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET2754280.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(1972..2904)
                     /locus_tag="VQ651_001280"
     CDS             complement(1972..2904)
                     /locus_tag="VQ651_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HET2754281.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(2901..3758)
                     /locus_tag="VQ651_001281"
     CDS             complement(2901..3758)
                     /locus_tag="VQ651_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2754282.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(3762..5108)
                     /locus_tag="VQ651_001282"
     CDS             complement(3762..5108)
                     /locus_tag="VQ651_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2754283.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMVIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSATEE"
     gene            complement(5121..5405)
                     /locus_tag="VQ651_001283"
     CDS             complement(5121..5405)
                     /locus_tag="VQ651_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2754284.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(5409..7439)
                     /locus_tag="VQ651_001284"
     CDS             complement(5409..7439)
                     /locus_tag="VQ651_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET2754285.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NSHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            7631..8638
                     /locus_tag="VQ651_001285"
     CDS             7631..8638
                     /locus_tag="VQ651_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET2754286.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            8641..8841
                     /locus_tag="VQ651_001286"
     CDS             8641..8841
                     /locus_tag="VQ651_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2754287.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            8993..9175
                     /gene="rpmF"
                     /locus_tag="VQ651_001287"
     CDS             8993..9175
                     /gene="rpmF"
                     /locus_tag="VQ651_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET2754288.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKITKAASAE"
     gene            9191..9340
                     /gene="rpmG"
                     /locus_tag="VQ651_001288"
     CDS             9191..9340
                     /gene="rpmG"
                     /locus_tag="VQ651_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2754289.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(9832..>9978)
                     /locus_tag="VQ651_001289"
     CDS             complement(9832..>9978)
                     /locus_tag="VQ651_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2754290.1"
                     /translation="YLNESGSMATGWVTVSGKWYYTYNSGDLLVNTTTPDGYRVNANG
                     EWVG"
BASE COUNT         3234 a         2118 c         1610 g         3016 t
ORIGIN      
        1 gttgacgtga tttgaagaga ttttcgagta tgagtttatt ttttacctga cttgtccata
       61 ttccagaagt ctgtcacggc tccgcgtgaa gcagatgata cgatgtgggc atatttaccg
      121 aggacaccac ggctgtaaag tggtggcaag gttgtttctg ccttgcgttt ttcaagttct
      181 tcttcggata cggccataga aatttctttg gtatcttggt caaccgtaac gatatcgccg
      241 gtacggagat aggcaattgg tccaccatcc tgagcttcag gagcgatatg tccaacaacc
      301 agaccataag taccaccaga gaaacgtccg tccgtcaaga gggccacctt atctccctga
      361 cctttaccaa caatcattga agaaagtgat agcatctcag gcataccagg accaccttta
      421 ggtccaacaa aacgaacaac gactacatcg ccatcaacga tttcatctgt cagaacggcc
      481 tgaatcgcat cttcttctga gtcaaagacc ttagctggcc caacgtgacg acgcacttta
      541 acacctgata ccttggcaac tgcaccgtca ggagcaaggt tcccgttcaa gatgataagc
      601 ggaccatccg cacgttttgg attttcaagt ggcatgataa ctttttggcc tggagtcaag
      661 tctgcaaagt cagccaagtt ttcagctaca gtcttaccag tacatgtgat gcgatctccg
      721 tgaaggaaac catttgccaa caaatacttc ataaccgcag ggacaccacc gacttcgtag
      781 aggtcttgga agacatactg accagatggt ttcaagtcgg ccaagtgagg cacacgttct
      841 tgaatcgtat tgaagtcctc aagtgacaag tcaacatttg cggcatgggc aatggcgagc
      901 aagtgaagag tggcgtttgt agaaccaccg agagccatcg ttacagtgat agcatcttca
      961 aaggcttcac gagtcaagat atctgatggt ttgagaccaa gttccaacat cttaacaaca
     1021 gcacgtcctg ctgcttcgat atcttctttc ttatcagctg attcagctgg gtgagaggat
     1081 gaccctggca aactcatccc tagaacttcg atagcagttg ccatggtatt agcagtatac
     1141 ataccaccac aaccaccagg gccagggcag gcattacatt caagacgttt cacgtcctca
     1201 gctgtcatgt caccgtggtt ccattttccg ataccttcaa agacagaaac caagtcgata
     1261 tctttaccat caagatttcc cggtgcaata gttccaccat aggcgaaaat agctgggata
     1321 tccatattag caatagcaat catagatcca ggcatgttct tgtcacagcc accgatagcg
     1381 acgaaggcat ccacgttgtg accactcata gccgcctcga tggagtccgc gatgatgtca
     1441 cgagatgtta gagagaaacg cataccaggc gttcccatag cgatcccgtc cgctacggta
     1501 atggttccaa actgtacagg ccaagcgcct gcagatttga caccttcttt agccagtttc
     1561 ccgaaatcat gcaagtgaat gttacatggt gtattttccg cccaagtcga aatcactccc
     1621 acaatcgatg tttcaaagtc cttatctgtc ataccagtcg cacgaagcat agcacggtta
     1681 ggtgatttaa ccatgctgtc ataaatgcta ctgcggtgac gtttatctaa ttcagtcatc
     1741 ttatccctcc catttcagtt tttactatta tagcacaatt ttcgcatgaa gaacagaata
     1801 aaattcttga attttcagaa aattctatac acatgtgaaa tatttaaaat taaaaacaac
     1861 aaagcggatt agtgcacttt ctgatgacca gaatatgctt tttaatccgc tttctttaaa
     1921 taacgtactg taatttttac agaaattctt tcaaataagt gtatttaaca tctatcttgc
     1981 attataaatt tctagaacct tctcttttat attcgattca ctcaaaccat actcattaag
     2041 aagataatcc attttcccta cttgaccgaa tctttcttga acacccatcc gatgaatttt
     2101 tgttattcca tcatcagaga ataattcaca taaagcactg ccaattccac ctatctgatt
     2161 gtggttttct acagtaaata tagtttttcc acttaacatt gtttttatct gttctggtat
     2221 cggtttgatt ctaaataaat ctatcacacc tactgaataa cctaatttag acagttcatc
     2281 tgcaactcga atacttggag caaccattat gccagaagca acgattacaa gatcttcacc
     2341 atgccttaac tcaatgtagc ctttagaaaa atcttctcca ccttgataca caggaactgg
     2401 agcttttcta attgttcgaa tatattttag tccttttaag tctaatgtct ggttcaatat
     2461 ttcacgaaat tggatatcat cagttgcttc gaaaatgatt gatttaggaa ttaaacgtaa
     2521 caatccaatt tcttcaaatg gcatatgtgt tccaccattc atctctgccg ttactcctgc
     2581 atctgatcca atcacagtgg catccaattg tgcgtatcca agagaaataa ataattgatc
     2641 aaatactctt cgtgaagcaa aaggaccaaa tgtatgaaga taaggtctaa acccctgaat
     2701 agacaagcct gctgcaaggc cgaccatttc tgcttccata atcccaacat tcacataacg
     2761 gtctccaaag tccttttcaa gattattagt agccatcgaa cttgacaaat cggcttctaa
     2821 gactactata tcagaatcac tttgattagc ctctaaaagg aagtctctat atacatgccg
     2881 taattctttc gtacttctca tcattctgtt tcctccaatt cctgacttaa tctttctaca
     2941 actgaagtta acatttgttt ctcctctaca gtagggcgaa gatgatgatt ggatttcatt
     3001 tcttccagct cttgaacccc ttgaccttta atagtatcta atacaatgca cttaggtgat
     3061 gaattatttg actgttttaa ttggacaatc ccttcataaa tttctctaat atctgaaccc
     3121 ttgaccctaa tggattcaaa tccaaatgct gaaaattttt ctacgaaatc acctggatta
     3181 caaatatcct ttgtaaaacc atctaattgt tttttgttat catcaacaaa tacaattaag
     3241 ttggataact gttgatgaga agcaaactgt atagcctccc aacattgtcc ctcatttaac
     3301 tcaccatctc caacaatagc gtaagtataa aagggactct ttcttattct ctgaccatat
     3361 gcaagtccag ttgcaacact aattccttgt cctaaagagc ccgttgtcat atctatgcct
     3421 ggcgttagat ttctatcagg atgagacggt aatttggttc catttgtatt taaagaatat
     3481 aagaattctt tgtcaaagaa accattcaaa tagagtgtac tgtatagagc tggtcctccg
     3541 tgaccttttg ataatatgaa ataatctcta tctcgtgctg caaatatttc tggagtcatt
     3601 ggcattattt caccataaag caccgctaaa acttctacga tagacagact tcctccgtaa
     3661 tgtccgaatc caagatgatt caatgttcta agagtattta atcggatgtt agtcgcaaat
     3721 tttcttaacc catcttctct atttttactt aaaatcatcc cttattcctc cgttgcagat
     3781 ggctttttaa taaaggatac tccaaacata actgctagaa taagaacaag accaatcaca
     3841 atgcctgctt gtgagccaaa ttgatttaac attcctaaaa taattcctga tagaccaaaa
     3901 tctgcatctg agaaagttga tccttggaaa ccaagtcctc ccaaaactgg cattaaaaag
     3961 actggaagaa aactgattaa aataccttgt aaaaatgctc caatagtggc tccacgaaca
     4021 ccaccagatg cattcccaat gacacctgca gtcgctccac agaagaaatg aggcacaaca
     4081 cctggtaaga taacaaccgt tcctgaagca atcataatta ccatacttac taaaccacca
     4141 acaaaactag agataaatcc aattagaact gcattgggtg cataagtata aacaatcgga
     4201 caatccaaag caggttttga attaggtaca agacgctctg aaataccttt aaaggctgga
     4261 acaatttcgc ccaaaataag gcgaacacct gctaaaataa caaatacccc tgctgcaaat
     4321 tgacctgcta attgtaaagc ataaactaga ccacttgtac cactactgat ttctttttct
     4381 atatattctg accctgcaaa gatagctaca ataatgtaaa taactgccat ggataaagta
     4441 atactaacag tactatcacg taaaaaagct aaactctttg gaaatttaat gtcctctgtt
     4501 gattttgatt tgtcaccgat aaggctacca gtaaaaccac tcaaccaata tcccaaagaa
     4561 ctgaaatgac ctaaagctac cttgtcattt ccagttaatt gaaccatata tttttgcaca
     4621 aatgctgggg aaatactcat aataataccg agtgctaatc ctcctagtaa gatgagaggc
     4681 aagctagtaa agccagcaac tgataaaatg accgcaatca tacatgccat atatagagtg
     4741 tggtgccctg ttaaaaaaat atatttaaat cgagtaaaac gagcgattaa gatattgaac
     4801 accatgcctg caaacataat cattgcagta gctgagccat atgttgttaa agctacagct
     4861 acaattgctt cattattcgg cacaacgcca gataaatgaa aagcatgctc aaacatggta
     4921 ccaaatggat ttaaagaatt ttgtacaatt cctgcaccac cagatacaac taagaaacca
     4981 acaaaggtct taattccacc tttaataata tcaggtaatt tcttcttctg aagaactaat
     5041 cctaagattg caattaaagc tactaaaata gctggtgtac taacaatatc caatatgaac
     5101 ttcatcatga cgctagcctc ctatataagt cctttttctt cacaaagttt agtaattaat
     5161 tctcgtagtt catccatatc aataatacta tttaagatac gaacatctcc aagatgacta
     5221 gctgaatcag ctagatcacg accaacaatc caaatatcag ctgcatttgg atctgctcca
     5281 cctaaatcat aatgttcaac ttctacatcc gaaacattca aatcactcaa tacagattca
     5341 atattcatct gtaccataaa acttgaacct aatcctgaac cacaagctgt accaattttt
     5401 aacattatct aatcctcctg tttaattatc attttaatgt catcatagtt ttttgatgat
     5461 attaaagttt gaacatgatt tttatctctt aaaattgttg ttaaatgtga caaagccttt
     5521 aaatgactct cattatcaat ggctgcaata caaatcaaca atcttacctc ttgttctgga
     5581 ttatccaata aataaatcgg ttcttccaaa actaacattg acattcctat ttcattcaca
     5641 ccttcatctg gccgagcgtg aggaattgct actcccttcc ctaaattaat aaaaggtcca
     5701 aactcttcta ctttttgaat cattgcctca gggtagttct cagttatctt atcttgatcc
     5761 aaaagcggtt tagctgctaa acgaatcgcc tccttccatc ctaatttttg cgaactaacc
     5821 tgataggttt ctttggtaat aagttgttct agcactggta caatttcctt tctatcattt
     5881 ttttggtaaa gataattctt taacgccaat cttaattcca attcttgtgt aataattcca
     5941 tatcttttga caatattcag gatttgttca atctcaaaat ctccatactc taaattcgga
     6001 aaatctttta acactagttc tactagttgt attgcttgct cttcagtcat cataaccgaa
     6061 actagataat ttggcttttc tgtctccacc tttatggtag aaaaaaccat atcatagtca
     6121 ctactagctt tcacctgtaa atcatcaatc tttgaggttc ctataaactc aatttgagga
     6181 aataatgcta atagattctc ttttaacatc aatgaagaac taacaccatt aggacaaatg
     6241 attgctgctt tataccattt ttgaggcaaa gtatctgctt tctttaaata acctccgaaa
     6301 tggataacaa aatatgctgt ttcactatca ggtatgggat tgtcaatagc gtccatcaag
     6361 ggcatcaaag aatctttgac taattcaaat aaatcaggat aatgttcttt aacatgcaat
     6421 acatattcat ttgaactagg taggccgaac tttaatctat agtaagccgg tataaggtgg
     6481 cggcgaagat tttctctcaa tccttccctt tgtttaaaat gtaacaaaga aatatcttcc
     6541 attctactta taatagcctc tgttaattga ttaaagtaaa ccggagcaac atctacttca
     6601 ccttcaaagc aacttgataa taaaacggtg atatagcgat aatcatcctc agaaaacacc
     6661 gtatctataa ttcccaaatc aaccactgta tccaataaaa tagtggttat atcttgaata
     6721 acaggagata ctaatgtctc tgaaagacat actctttcaa catccctttg atacctacac
     6781 agaatgaata ctaaaccgaa aaggtaaact tttaattgat taacaatagg tactagctgt
     6841 agcttctcat aataatcttt aactacctga tcaatcaaat cataagttaa tgaatacccc
     6901 caactggata aaacataatc caaaccccaa atccctatgg aggattccag caactcacta
     6961 accatttgaa aagctaagcg gtgcttattc cactctgaac cgtgtaaagt ataacctttt
     7021 gctctactgt accctagctc caaatcatta tctaacataa tctttcttaa tgattgaata
     7081 tcagataagg ttgtattctt acttactttc aaaaagtctt ggtaatgact attcgatata
     7141 aaatctaatc ggcaaaaagt gtaaagatag attaaagcta agcgagtcga ctttggtaaa
     7201 accaattcat ccgacttaat aatatctgtc aaagactgct tcgtacgatt tgataaacta
     7261 tagcgacctt gctttttatc cagcactatc cctttattag ctagataagg cactaaataa
     7321 tctattcctt ctttgacttc ctttataggt aagctcacct taacagataa ttcatataat
     7381 gatagctcac aatgatccat caaagtcatc aaaataacta gtgctctata atcaaacatt
     7441 tctcttcctt tctaactaca attatagtgt aaaaattata agaataaaag agtatttgag
     7501 cacaatataa tctcaccttt taaaagaaat tattgggctg aaagttttta gtgattattt
     7561 ttcaactttc tattgacttt ataaattaaa gtgatatcat ttagatatca aataacagga
     7621 ggtcattctt atgactgaaa aactaatcaa ttcaaaacca aatggtgtat tcgcattgat
     7681 tctcattgag ttgacaatcg tacttggtat ctttatattt ataatggggg ttggttcgga
     7741 aaacattttt ggaattatta tcggaccttt actaatcgta attgcaggtc tagctcatgc
     7801 tggtttaaaa gttgtcaaac ctcaagaagc tctggttctg acactctttg gtaactatac
     7861 aggtaccatc aaagaacctg gcttttactt tgtcaatccc ttcagcgtag cagtcaaccc
     7921 tgcaaaccac actcgacttg gacaaagtgg tgatgttagc acaaaatctc cttttttagg
     7981 agctaaatca tcaaatgaca atgatgtaaa tcttgaaatt ggcaagaaac agatttccct
     8041 caaagtcatg accttgagca attctcgtca aaaaatcaat gattgcttag gaaaccctgt
     8101 agaaatcggt atcgcggtaa cttggagagt tgtcgatacc gctaaggcag tcttcaacgt
     8161 tgataactac aaagaatatc tttcattgca atgtgatagc gccctccgta atattgtccg
     8221 catctatcct tacgatgtgt ctcctaatgt ggatactacg ggtgatgggc aagcagatga
     8281 aggtagtctc cgtggctcta gcgaaattgt tgctaaccgt attcgtgaag aaatccaaag
     8341 tcgtgttgag gatgctggct tggaaatcct tgaagcacgt atcacttacc tagcttatgc
     8401 tccagaaatt gctgccgtta tgcttcaacg ccaacaagca tctgccatta ttgatgcacg
     8461 gaagatgatt gtagatggtg ctgtaggaat ggttgaaatg gcactagaac ggctcaatga
     8521 aggggaattg gtagaacttg acgaagaacg aaaagctgcc atggtttcaa atctccttgt
     8581 cgttctttgt ggcaatcatg atgcacaacc aattgtcaac acaggaagcc tttactaaag
     8641 atggctgaag ctaaaaaaaa gcagatcccc cttcgactct caacaaagtt atacgctgca
     8701 ctcgcatcat gggcagaaga cgactttcgt tcagtcaatg ggcaaatcga atatctcctc
     8761 acagagtgtg tcaagcaacg gaagaaagac ggaaaatacg tatcagaaac cattgacgaa
     8821 ccatttgaga ttgatattta acaccttctc ctgtctgccg tgtcggatag gagaattttt
     8881 tcatcttttt ttgtcaagta actttacttt acaaaaaaaa tgtgttatcc tagtatggtt
     8941 gatgaaaatc agtagattga atcgaatata aatacctaaa ggagaaatca aaatggcagt
     9001 acctgcacgt cgcacttcaa aagcgaagaa aaacaaacgt cgtacacact acaaagtaac
     9061 agctccatct gtaaactttg acgaaactac tggagattac tcacgttctc accgtgtatc
     9121 acttaaagga tactacaaag gacgtaaaat cactaaagct gcatcagctg aataatagaa
     9181 gggagatacc atgcgcgtaa atattacact tgaacacaaa gaatctggtg aacgcttgta
     9241 ccttacttct aaaaacaaac gtaacactcc agaccgcctt caattgaaga aatactcacc
     9301 aaaacttcgc aaacacgttg tgtttacaga ggttaaataa gcattcaata cgaatcaata
     9361 cagaagaaaa acgcttgatc agcgtttttt ctttttgtta atttcactac attgtaaggg
     9421 aaatcattcg aatgactaca tttttcacta ccttttttga aataagaata cggattcgga
     9481 caattaaaag caagaggaac tcaaatgtaa gttggggttc cttttttgta tatttttcaa
     9541 ataacgcctc tacactattt gtagcaaatt caccaactac agttgtatct tagttaaaat
     9601 aagttagaat atgtaagtga gtaccagata taccaagaca tcgtcaccat ctaaggtata
     9661 ttcaaaatac aaaagttgac caactagatt tctgaatatc cttatatatc cattcttaaa
     9721 attggtttaa atagcgtagt cttttaaact agttttgaga atccaaaaaa tcttcctaca
     9781 tatgtaagaa gattttttag ttcagaatga ttagatttag ctaatggata cctatcctac
     9841 ccactcaccg ttagcattga ctcgatagcc atcgggtgta gtcgtgttta ctaataaatc
     9901 tcctgaatta taggtatagt accatttacc agaaactgta acccaaccag tagccattga
     9961 acctgattca tttaagta
//
LOCUS       DAVBHS010000075         9917 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVBHS010000075 DAVBHS010000000
VERSION     DAVBHS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9917
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.075"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            23..610
                     /locus_tag="VQ651_001290"
     CDS             23..610
                     /locus_tag="VQ651_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2754291.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            812..949
                     /locus_tag="VQ651_001291"
     CDS             812..949
                     /locus_tag="VQ651_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HET2754292.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            1128..3737
                     /locus_tag="VQ651_001292"
     CDS             1128..3737
                     /locus_tag="VQ651_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HET2754293.1"
                     /translation="MDYNFNLEHPFFFTNNDYSTDTSIKYQVSLPFNWHEVMNNDEWV
                     YQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICHEMRIPFKHLSTEDKFIMRNGK
                     LVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYILSDKRWDEAPIYLRYGVFR
                     PSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPDFLNKWLDKKDKKQGDFPFI
                     IDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFDGTYSSEKLASECKALKILN
                     EWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFPLYEVVDKTKDYLLRVSKIV
                     EKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWEQAVFSNDEKVVHKVAAPGF
                     RAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVSQTRIEGKKLFESLGYTREH
                     IDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIESEQDIQDFIIKLLRGFTLTYG
                     QWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLAKKVKNFKFDDYYEIRTQVI
                     NEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVELFTTHFIEIFENSQNKNLYSG
                     QAGILLVGLYFLSKGEIDNKLGEEILIRLREYTLNYIENPETFCKVGASDVQSNDPYE
                     NFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVDKELVAYKVDSNNSLQYSQGHR
                     LLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERATDVVFCVLPGLFNGFCGLEVA
                     NNIYSDIDDNFSGQKKLIEQLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAIGLV
                     SIMDNKLTILPQI"
     gene            3770..5710
                     /locus_tag="VQ651_001293"
     CDS             3770..5710
                     /locus_tag="VQ651_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET2754294.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEELFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGLSSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDRVLLFLERRTFYEKILSFDLIENTLKTEFERPIIKSNNTKYFSKVIWTKKDSYD
                     VSIPISLFWKSEDTDELPRRKKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHV
                     RGGGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGA
                     VLNEEKNLLQSILLFSPFINPYDTLQNPNDPLSKTEIAEWGDIRDPEVKAYIKSYSPM
                     QNIEKARDSNTVIVNILGEKDPYINNNEVIEWSKKLNSIGVKSLLYLNKAAGHGGFTP
                     SDVLLMIDTLNYFFEEVGRNNL"
     gene            5707..7314
                     /locus_tag="VQ651_001294"
     CDS             5707..7314
                     /locus_tag="VQ651_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754295.1"
                     /translation="MNYLKFIKKTKLILMGILIFLSSFNGVLLSGIIVYAGSLNQTSS
                     FSDVLRFGAISILGWSAIYISNYYLEVTEASITKDINVKIKQGYFREQYLSSEMVKDY
                     SSIISVLSNDLRLIEENYFRQIFEIISSILLFIVSLSFMLYLNFLVSIIFIVLSALPI
                     IVPVFMKKMLSNSANEYSNSNAEYTHIIKEIFNGFKTLKSYSVTKEIISLSDKKLDKL
                     EDSTFNLKRSEVLSKLVAVLISGFCFLVPLVVGCYFVIYHKSLSFSELIGIFLANDKV
                     LGPIQSIAYSLNKINTTKDLRKPFLKYLSGEKNFIDAEHDNNGLYTSSIDEIHMKDVV
                     YSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLINGSLKPQKGYVNLLSHGKKS
                     SDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVLKKVNLYEELEKIDILNYQC
                     GENGSNLSGGQKQKIALARALIRNNKVYLFDEISANLDNDNSNSIHDILFNLGISFIE
                     VSHHYDLNDKRYTDIYKLENGTLFKIK"
     gene            7329..8069
                     /locus_tag="VQ651_001295"
     CDS             7329..8069
                     /locus_tag="VQ651_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754296.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            8072..9391
                     /locus_tag="VQ651_001296"
     CDS             8072..9391
                     /locus_tag="VQ651_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754297.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIASKWNLLYRKPY
                     IKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIIS
                     IAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLTFVTSLIAFLLNIKIMALIYY
                     SLEDVLIDDMNLLGLILPNFIVSIILFILIFITKSSYFIFKNAKIIS"
     gene            9525..9662
                     /locus_tag="VQ651_001297"
     CDS             9525..9662
                     /locus_tag="VQ651_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HET2754298.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
BASE COUNT         3365 a         1237 c         1891 g         3424 t
ORIGIN      
        1 aagatttatt tcggaggtaa ctatgaaaag aataattcca gtttatatat tccaacaagt
       61 aaatgtccta ttggtatctc tatacttatt gaaatttctt tgtatcggtg agttaactat
      121 actacagatt ctctatggtt cgtcactcat ttctttttta tggatgtatg gccaacgcaa
      181 acaagcgcat aaggtcaata tgaaatctag gatgaaatgg cttggtgttg aatttgttag
      241 cctactgatt ataagtctat gttttagtct gattcatgct caaggaagca cgaatcaagc
      301 aaacttaatt ggccttcaac atcaaattcc ttggttttca tttttattgt tcttaatcaa
      361 tgcgagtatg gttgaagaat ttctgtatcg agaaatttta tggaatttgg tcagaaaatt
      421 agatatcaga gttgctttga caagtgtttt atttgcctta gcacatcata caggaaccat
      481 tctagcttgg tgtttgtatg tttcacttgg gatgttttta ggtatggtgc gctataaatc
      541 tgacttatgg ggcagtatgg gactacattt ggtgtggaac ctactagttt atagtttgct
      601 gcttttttaa aataggtgac ctgattttta ggtaacttct aaaactaact tccagttttg
      661 ggagagagat ggaagttact ttgaaaagtt acggaaacat tattttctat gtgacaacat
      721 tgtataattg cttgcatttt actggtggag agagtataat gagggtggat aaagtactta
      781 atcccaaaaa tttaaatagg aggagctagc tatggcagaa gaagtattaa atcttcaatt
      841 agtatctgtt caagttgacg aaacagatga agttgatgga atgcgattta gtacattcag
      901 tacaaatcgt tgcggtaact ggagtgcttt tagttgggaa aattgttaat tcatttaaat
      961 tgtaaacttt agagattctc tggagttatg gatgtcgttc caatatgtgc acgttggaat
     1021 gttagtgctt atatggggag aatccaaagg attctccctt tttatttaaa taagtctgtc
     1081 tagctataga tatattaagt ttgataaaat ttcttggaga ttttgttatg gattataatt
     1141 tcaatttgga gcatcctttc tttttcacta ataatgatta tagtacagat actagcataa
     1201 agtaccaagt aagtctgcct tttaattggc acgaagtaat gaataatgat gagtgggttt
     1261 atcaataccc tattggaaaa tttgttgaaa gacaagggtg gaaaatacac attagttcag
     1321 agtataacag ttcacatgaa ttgttacaag atgtagcaaa aatttgccat gaaatgcgaa
     1381 ttccatttaa acatcttagt acagaagaca aatttatcat gcgaaacggg aaattggtta
     1441 gtcgaggatt ttctggtaaa tttattacat gttatccaaa tcaaaatgaa ttagagtcgg
     1501 ttttgcaaag gttagaaagt gccttaaagc aatataatgg gccatatatt ttaagcgata
     1561 aacgttggga tgaagcacca atatatctga ggtatggtgt gtttaggccg tcaagggatg
     1621 atgaaaagaa ggttgctatt gatgaattaa ttgttggcga tgaagttgtt aaagatgaac
     1681 gtttaccagt atttaaaatt ccaaaaggta tagtgccccc tgactttttg aataaatggc
     1741 ttgacaagaa agataaaaaa cagggagact ttccctttat tatagataat gcaattagat
     1801 tttcaaacag tggtgggata tataatgcta ggctaaaaga agatgggaaa aaaataattt
     1861 taaaagaggc tagaccgtat acaggtctag gatttgatgg tacttattct tcagagaaat
     1921 tagcttcgga gtgcaaagca ctaaaaattc taaatgaatg gagtgagacg cctaaaatat
     1981 actggcacgg taagatctgg gagcacacct tcttaggaat tgaacatatg aagggcgttc
     2041 cactaaatag atgggttacc aataattttc ctttatatga agttgttgat aagaccaaag
     2101 attatctatt gagagtttca aagatagtag aaaaactcat cgatttaacg aataagtttc
     2161 attctgaaaa tgtatatcat caagatttac atttaggaaa tatcttggtt aaggatgaag
     2221 atgagattag tataatagac tgggaacagg ctgtcttttc aaatgatgaa aaagttgtac
     2281 ataaagttgc agcccctggt tttagagcat ggcgagaaac acttcccagt gagatagatt
     2341 ggtatggtat tagacaaata gctcattatt tatacatgcc actagtaact acatcggatt
     2401 tgacatacaa ctatgtaagt caaaccagga tagaaggaaa aaaactattt gagtctttgg
     2461 gttatacaag agaacatata gattatgtag aaagtttatt gtcatatcta gattctaaat
     2521 gtccacagat tgagaatatt tccaggaaaa aagttttaaa acccatgcat gagattagaa
     2581 ctatcgagag tgagcaagat atacaagatt ttattataaa attattgaga gggtttacgc
     2641 taacttatgg acagtggcgt aaagaatttc aatcaagatt cttcccagtt cattattatg
     2701 gtctgaattt taatcaagga attgcgtttt cagatttagc tatcctttgg agttatcaac
     2761 aactagctaa gaaagtaaaa aatttcaagt ttgatgacta ttatgagatt cgaacacagg
     2821 ttattaatga ggcagtaaat aattttaaga aatcgtccct ttctggtctt tttgatggta
     2881 aaattggtac aatctggctc atttatgaat ttggggagat agatcgggca gttgaacttt
     2941 ttacaactca ttttatagag atatttgaga attcacaaaa taaaaattta tatagtgggc
     3001 aggctggaat tttactggtt ggactgtatt ttcttagtaa aggggaaatt gataataaac
     3061 taggagaaga aatactaatt aggcttagag aatatacttt aaattatata gaaaatccag
     3121 aaactttttg taaagttggc gctagtgatg ttcagagtaa tgatccttat gagaattttg
     3181 gtggattact atatggtcac gcaggggtag cttggctctt cggtgaagct tataaattaa
     3241 ctggtgaaag tatctataaa aatggtctag aattggctgt agataaagaa ttagttgctt
     3301 ataaagtaga tagtaataat tcattgcagt acagccaagg tcatagatta ctaccatatc
     3361 tagcaactgg aagtgctggc ctgcttttac ttattaatag aaataaagaa atcttgtcaa
     3421 gcaaatactt aaaatattta acttctttag agagggcaac tgatgtagtt ttttgtgtat
     3481 taccaggttt atttaacgga ttttgtggtc ttgaagttgc taataatata tatagtgata
     3541 tcgatgataa cttttcagga caaaagaaat taattgaaca actttataga tatttgtgtg
     3601 taattgaaga aggatttgtt attgcgggag ataatggttt aaagataaca acagatattg
     3661 cttcgggatt tgcgggtgtt gcaattggat tggtatcaat aatggataat aaattgacga
     3721 ttcttcctca aatataatat ttagatacca ttattaaggg gtcatacttg tgaatcagag
     3781 taatgaaata tttttaaata caatacagga aaaaacacac tataagaggt ctatctcttc
     3841 atcatttcag gaattaaaaa aatggaattc tttgatattt aggagagggc gatattggta
     3901 ccaatttgat aattggagta tacccaatac tggtgtaata aaggaatggt atgtcgaaga
     3961 taatgactgg attcctaagg ttagttcttc cagtttagac aattcaaatt cacttaaaat
     4021 tgtctttgat gtggaaaaat atactttgcc agatgatagt atcttacagg atgttaggat
     4081 ttcatctgat gagagttata tagttttggt tgtatcctca ctgcgtagca ctaatttaat
     4141 tggaataaaa aaatatacta tggaagagtt atttatcatt gaagatattt cagtagaaag
     4201 ttcgttctat ttgggtaaat tcggtgttat gtatactcgc tccaaagagt acgggaggcc
     4261 aagtaagtta ttctataaat cttttgatag ctttacagaa gaggaactgt ttgaagaaaa
     4321 cgaatgctct tttcgattaa aaatagtcca cattgatagc aataattgtt ttgtaaagtc
     4381 agtagacttt cagaaaggca ggatttttct atacagtttc gaccggacgg gttttgttag
     4441 acactcttac acagaaacgg tagccccaac acctagagat atcgctctat ttagtacaga
     4501 aaaagcacag tactttcttg ggctctcttc aactgaagaa aagaaagatc aactaattct
     4561 aagggaaact tcttcgggag acagagtttc tattactata ccatatcaag atagggctag
     4621 aagagttcac tgtataggac gttatatcct tttagactgt tcaaacgcag cgaattccgt
     4681 cttttatctt gctacattta aaaataatag tttgagggag ttgagtgtta ataagataac
     4741 atttgatgaa aagacaacac tttatgaaaa ttcattttct gatcgtgttc ttctgttttt
     4801 agagaggaga actttttacg aaaaaatact tagttttgat ttaatagaaa atacattaaa
     4861 aactgaattt gaaagaccca tcataaaatc gaataatact aaatattttt ctaaggtgat
     4921 ttggactaaa aaagacagtt atgatgtaag cataccgatt tctttatttt ggaaaagtga
     4981 agatacggat gagctcccaa gaagaaaaaa atgtattcta agtgtctatg gggcttatgg
     5041 taagaatgat aattctgatt tagatgaaat tatgttatct ataatagatg caggttttat
     5101 ttatgctata gttcatgtaa gaggtggagg ctatttgggt ggtgaatggt atcgctcagg
     5161 aaaggcgtta aacaaatgga attctatcag agattttatt gagggagtaa attatttaag
     5221 ggaaaatgat gtaattgaca gtaagcgatt aggtttaata acttctagtg caggtggaat
     5281 aattgctggt gcggtgttga acgaggaaaa aaatttattg caaagcattc tcttattttc
     5341 gccatttata aatccttatg atacacttca gaatccaaat gatcctcttt ctaagaccga
     5401 aatagcagag tggggagata ttagagaccc tgaagtaaag gcttatataa agtcatattc
     5461 gcctatgcaa aatattgaaa aggcacgaga ctcaaatact gttatagtta atattttggg
     5521 tgagaaagac ccatatatta ataataacga agtgatagag tggtcaaaga aattaaattc
     5581 tattggagtt aaaagtttgt tgtatttaaa caaagcagct ggtcatggag gttttactcc
     5641 atcagatgtt ctcttaatga ttgatacttt aaattatttc tttgaagaag tgggaaggaa
     5701 taacttatga attatttaaa atttataaaa aagactaaac ttatattaat ggggatttta
     5761 atatttctat cttcgtttaa tggtgtcttg ctctcaggaa ttattgtata tgcaggtagt
     5821 ttaaatcaga cttcctcatt ttctgacgtt ctaagatttg gtgctataag tattctgggt
     5881 tggtcagcaa tctatatatc aaattactat ttagaagtaa cggaagcatc aataacgaaa
     5941 gatataaatg taaaaattaa acaaggatat tttagagaac agtatctttc ttctgaaatg
     6001 gttaaagatt actcatctat tatttcagtt ttgtcaaatg atttgagatt aatcgaagaa
     6061 aactatttta gacaaatttt tgaaataatt tcttcaatat tgttgttcat cgtctctcta
     6121 agttttatgc tttatttaaa cttcctagtt tcaataatat ttattgtatt atcagcattg
     6181 ccgattatag tacctgtctt tatgaagaag atgttgtcta attcagctaa tgagtactct
     6241 aacagcaatg cagagtatac tcacataata aaagaaattt ttaatggttt taagacatta
     6301 aaatcttatt ctgttactaa agaaataatt agtttgtcgg ataaaaagtt ggataaacta
     6361 gaagattcta cttttaattt gaaacgatca gaggttcttt caaaattggt tgcagtacta
     6421 atttcaggtt tttgctttct agttcccttg gttgtaggat gttattttgt aatttatcat
     6481 aaaagccttt cttttagtga actgataggt attttcttag caaacgataa agttttaggg
     6541 ccgatacaat caattgccta ttcattaaat aagataaata caaccaaaga tttaaggaaa
     6601 ccgtttttaa aatacttaag tggagagaag aattttatag acgctgaaca tgataataac
     6661 ggactgtata cttcatcaat agatgagata cacatgaaag atgttgtata ttctattaca
     6721 ccagaaaata aattaagtat tgacttctca tttaagtcac catttagggt attattaaca
     6781 ggaacttctg gtagtgggaa aacaacgatt ttaaatttaa ttaatggttc tttaaagcca
     6841 caaaaaggtt atgtaaattt gttatcacat gggaaaaaga gttcagattc aataccaaca
     6901 gttgatcaga caccatatat ttttgacact actattcgtg agaacgtaac tttatttcaa
     6961 aatgaatatt tttcagatga tcagataatt gaggtgttaa aaaaggtaaa tctatatgaa
     7021 gaattagaaa agatagatat actaaattat caatgtggtg aaaatggtag taatttgtct
     7081 ggaggtcaaa aacaaaaaat agctttagct agagctctga ttagaaataa taaagtgtac
     7141 ttatttgacg aaatatcagc taatttagat aatgataatt caaattccat acatgatatt
     7201 ctgttcaatt taggtatttc atttattgaa gtttcacatc attatgactt aaatgacaag
     7261 agatacactg atatatataa attggaaaat ggaacacttt tcaaaattaa gtgattggag
     7321 gcaagaatat ggataaagat tatatattaa aagtgaaagg gctgtatcat caatttttac
     7381 taggaaataa taaaacgttg caagtgctga aaaatgtttc tctttctgct tcgagaggag
     7441 aatttataag tattctagga attagtggtt ctggaaagtc aactttatta aaatgtattt
     7501 ctagtttgct tgaaccaaca agtggggaag taattttaaa tggaatcaac ccctataaaa
     7561 tcagaaatgc aaaattgtca agtataagac gtaacgaagt atcttttata tttcaagcat
     7621 acaatttaat accttccctg ccggtaatag aaaatatagc acttcctttg cgattatcac
     7681 aaaaaaaatt aactattaaa aatgtagaaa acttactcaa aagaatgaag tttaatgctg
     7741 gcttaaacga ttttgttgga actctgtctg gtggagaaca acagaaggtt gctatagcta
     7801 gagcggttat tgctgatagt gatataatat ttgctgatga gccaactggg gctttagaca
     7861 gcgtttctcg tgaagtaatt tttgaattat tgagagagtt agtaggggcg ggtaagtgtg
     7921 taattatggt aacgcacgat atagaattgg cctcgaaaac tgatcgtgca ttaatattga
     7981 aagatggaaa aattttcaaa gaacttcata gacctagcgg ggaagagttg tataaaatct
     8041 tagaggtaca atcaactacg gaggaataga gatgctcaga ttagtttact atcaattttt
     8101 acataataag aaacaatggt taggcgtttc accagtaatt tttgtttcga gtttagttat
     8161 gggattggct gttaatgggg taattaatgt tgagaataat tcgcaagtat ttgtcggact
     8221 tccagatcct aaaccaattt ttatgtttcc gatagtattc ggaggagtaa cattattctt
     8281 tgtgctctct aacataataa atatgttggt tgaaattttt agagatgatt acgaacttct
     8341 ggaggtgttg ggagcaagtc tccttcaact atcatttctt gtggggggac agatttttat
     8401 catttcctcg attattagtt ttattgcata tctatgctct atatttgtta cgtcaaatta
     8461 ttattatttt ttacaatact ttttcgggga aaatatatta cctgatatcc aatttcaaac
     8521 gagtgctgtt ggatgtatca ttacagtagt gttgatttca tttttagcct ttctaagtgg
     8581 ttgcttttat acgttcaaga agattagaaa taggaagagt tctaaaataa ggcatgttct
     8641 ttcaatagta aaaagaatac tgctattggc tggttttagt gttatctggt tgctttcttt
     8701 gcaacaaatt ttccaagata gcacaatttt agcgaaggcc cagataatat tcaatattgt
     8761 aatcttagat attgtgatta tttatcaatt atctcctttt atacagtcat gttttatcaa
     8821 actactttct ataattatat ttagaaataa ttttatgttc attgcttcga agtggaattt
     8881 actatatcgt aagccttata taaaatctat cagtgctgca attacaggag ctatattact
     8941 aatttctagt tttcaaatga tttctcagaa tatactatct caatttcaag atgacagtga
     9001 tttagagcta aaagtggcat ttattgtcta tgtaggagca ccaattttga tagtacttgc
     9061 aaatattatt tcaattgcat ttttgtcatc gcatcaagaa aggattgaga ttcagcaatt
     9121 tgaaatttta ggaacttcta attaccagat ggttaagatt aaagttggtg aggctatatt
     9181 tttgacattt gttacttcat taattgcatt tttacttaac attaaaatta tggctttaat
     9241 atactactct ttagaggatg ttttaataga tgatatgaat cttttggggt taatacttcc
     9301 aaattttata gtgtctatta ttttatttat tttaatattt attactaaaa gttcctattt
     9361 tatctttaag aatgcaaaaa ttatttctta aaaatttgta agttttcgct atataaaata
     9421 gttaaatcta ttttgtatca ttgcttgcat tttactggtg gagagaatat aatgagggtg
     9481 gataaagtac ttaatcccaa aaatttaaat aggaggagct agctatggca gaagaagtat
     9541 taaatcttca attagtatct gttcaagttg acgaaacaga tgaagttgat ggaatgcgat
     9601 ttagtacatt cagtacaaac cgttgcggta actggagtgc ttttagttgg gaaaattgtt
     9661 aattcattta aattgtaaac tttagggatt ctctgaagtt atggatgtcg ttccaatatg
     9721 tgtacgttgg aatgttagtg cttatatggg gggaacagaa tcctctcttg attgaagaca
     9781 agttagccat taggctggtt tgtctctttg tcaactgtag tgggttgata taatagtatt
     9841 agtgagtggg ataaaagttt catttagttt attcagtaca aatttaacgg gtcaagattt
     9901 attgaactgc accccaa
//
LOCUS       DAVBHS010000076         9661 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVBHS010000076 DAVBHS010000000
VERSION     DAVBHS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9661
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.076"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            172..267
                     /locus_tag="VQ651_001298"
                     /pseudo
     CDS             172..267
                     /locus_tag="VQ651_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(520..1140)
                     /locus_tag="VQ651_001299"
     CDS             complement(520..1140)
                     /locus_tag="VQ651_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2754299.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1140..1622)
                     /locus_tag="VQ651_001300"
     CDS             complement(1140..1622)
                     /locus_tag="VQ651_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HET2754300.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1622..2464)
                     /locus_tag="VQ651_001301"
     CDS             complement(1622..2464)
                     /locus_tag="VQ651_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HET2754301.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2559..3359)
                     /locus_tag="VQ651_001302"
     CDS             complement(2559..3359)
                     /locus_tag="VQ651_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HET2754302.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3503..4882
                     /gene="glmU"
                     /locus_tag="VQ651_001303"
     CDS             3503..4882
                     /gene="glmU"
                     /locus_tag="VQ651_001303"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET2754303.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4892..5437
                     /locus_tag="VQ651_001304"
     CDS             4892..5437
                     /locus_tag="VQ651_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2754304.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5449..5763
                     /locus_tag="VQ651_001305"
     CDS             5449..5763
                     /locus_tag="VQ651_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HET2754305.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5780..6472
                     /locus_tag="VQ651_001306"
     CDS             5780..6472
                     /locus_tag="VQ651_001306"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HET2754306.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6549..7040
                     /gene="rocS"
                     /locus_tag="VQ651_001307"
     CDS             6549..7040
                     /gene="rocS"
                     /locus_tag="VQ651_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HET2754307.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7138..7728
                     /locus_tag="VQ651_001308"
     CDS             7138..7728
                     /locus_tag="VQ651_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET2754308.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGVLDLSQLFD"
     gene            7737..9500
                     /locus_tag="VQ651_001309"
     CDS             7737..9500
                     /locus_tag="VQ651_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET2754309.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         2980 a         1681 c         2066 g         2934 t
ORIGIN      
        1 tcattttttt aacttgatac tcagtgaaaa gcaaagatta aactaggaag ctagctgtag
       61 gctgctcaaa gaacagcttt gaggttgcag ataaaacttg tgaggtcacc aacatatata
      121 atgtgaagct gacgtgactt gaatagattt tagaagggta tgagtctgga agttttaatg
      181 gataatgcaa gattccatag aatgggtaag ctagagttct tatgtgaaga gtttgggcat
      241 aaacttttac cttttcctcc ctactcatct tagtatagaa aagtgaatct aaaatagtac
      301 ataactgctt ctaaaacatt cttataaatt gatttaaatt ctcaaatcat attattcagt
      361 tcttatttca ttttgctcta caatcctgtt gagaagacac gtgttcatat caaaaaggta
      421 ttggcaagtt gcaatacctt tttacaaggt tcttttgtct tatttttgtt tcaactgact
      481 atatctccta tggttctagt tcagaaggct aggctataat tatgattgat aagaagtatc
      541 attccaagta ttgagagtga atgtttcaaa atcatgggtt tctataatgg tcaggctggc
      601 atttgctaga ccgccatctt tacgaagaag tggttcttta tagcctagga gagtacgaag
      661 actggcagta agattggcgc cgtgtccgac aattagaata cgctcagctg gactatcttt
      721 taatgatttg ataaattgga tggtccgttg agttgtacta tagagggatt cggctccgaa
      781 cattcgagtg tcaaattgag caagatttga acgaaaagcc tggatttgtt gcgggtaaat
      841 agcttccaag gttgcaattt tcaaaccttc taacttccca agttgccatt cacggagatt
      901 aggaacgatt tctaaagaac agggggtata gagttgactt tggataatct cagcagattt
      961 gaccgctcga ggtaaatcac ttgaataaat ctgatcaaaa ggaatttcct tgagatactg
     1021 gccaagtcgt tttagggttt caatggattc aggaagaaga ggagaatcac cgctagcacc
     1081 ttgaaaacga ccttcttggt tccagagggt acgaccgtgg cggacaaagt agagtttcat
     1141 tacttgatgt cctccaaaat atctacaaag tctgccttta caaagctagc caagtcttgt
     1201 ggcgcgacga taatgctgtg tccgacttcg cctgcagaga caatcattcg atccaaatct
     1261 agagcaattt tatcgataaa aatgggataa ttgtgtttct gacgaattcc gacaggatta
     1321 ttggctccat gaatgtaacc agttgttttt tctaagtcct tttgtggaat catgctcact
     1381 tttttattgc cagaaatttt agctagtttc ttttcagaca agtgctgagt gatagggaca
     1441 attccgataa tcggtccggt cttgtctccc aaaagcgcca aggttttgaa aatctgatct
     1501 cgttcataac cttgaggaag ctctccttct agggcattga tttgaatccc ctgatgaggg
     1561 atagctgctt tagataggat ttgttccacc aatgtttttt tgattttaac tttttttgcc
     1621 attatttata tttatcctcc aattgactca tccaaatacc aagccagatt cccagcgcaa
     1681 agaagaaggc gatgatgaca taaccgacaa gtgaaagtcc tgtgtattgg atactttcag
     1741 cgtttcctgc atttggaatt aagatcaaaa gggtacttga taggacgata ccgatgatga
     1801 aatgatagac gcgtgagtgg tagttgttta aggcataatc catcaatttt gaaaaaacga
     1861 tgagagttgc acctgcacca attccaatcg gaaagaaggt tcccaagaga tcaaaagttt
     1921 taaaaccagt caacatagga gcatagagtc ccaaaatcaa aagtaagttc gatgggctga
     1981 ggccaggaac caagacgcca agggccaata gtgcgcctgc taggacgaag ttaagaaagc
     2041 tggcgcttaa ggttccaacg acaaaattta aggcatagag tcctaatcca gaaatgataa
     2101 aggttgtcca taaccaagct aaatcaatct tgtctcggtc agattctcga gttgattctt
     2161 tgaggaggct aggaactgta ccgataatag ctcccgcaaa gctccataat acaaagacct
     2221 gataattttc aagcaggtat tcaatcgggt aggaaaataa gccgattccc agaagcatac
     2281 cgatggcaac tggaataaag tacaaaacat tttctttaag gtctttaaag ggatgggcca
     2341 gaaagccaat cattcgttca tagattccta agattgctgc tagaacccct ccggaaattc
     2401 ccggtaggat aaatccaaga gcaattacaa tccctttaat aacgcgtgct aaccatgaga
     2461 gcatatattt cctccaacta tttctatttc aaaaattcct tactatattg tatcacaatc
     2521 agacacaaaa agaaaaagca aatgataaac aaatgctttt aaggttttaa aaaaagtttt
     2581 cgaaaggttt cttctttatt ttttaaggga gagataacgt tgatatctaa atcgtggtca
     2641 aagccggcaa tttttccttt agatgtgtat tggtgaatat cataatctaa atcagtttta
     2701 ggactgctct ccaaaaatcc tgagtctgag ccgtaggacg gaatccaaac agaggtaaac
     2761 ttgcctgtat caatactgtg ttcttccatg aagtagacac caacgtagat gccgatgttt
     2821 ttagcaccta gtgatgctag ttttgctcga aagttttcga caccttcgtt catattagac
     2881 atggttttgt cttccacgtc aagccaatag taactagggc tgtaaggaga ggcagcattg
     2941 tagaaaactt cggcagcctt ttccatttct tggacacttt ttccagctac ataagcgtag
     3001 acagcaactg ggacattccg cttttgaagt tcagtgatat gactcttata ggccttgtct
     3061 attccattga taaatgaagc atcattttct tttgttgttt gagcaccact gtgaacacga
     3121 acaatagcac ctgaaatatt ttgtgagagg gcatcgtagt tgatttcctc aggacgctgc
     3181 cagccagaga ggtcaataat cggtttgtct aagtgtttca aagcctgtgc ttcaatctgt
     3241 gctatattgg attttgtttt aaacgattgg ctgtcattaa gtgggcgatt gatgattaaa
     3301 atgaacatca taatcccaaa aaaactaaat aaaataagtg gatgaatttg ttttctcata
     3361 tcttataagt ctaccctaaa aatcaaaaaa aatcaaaaaa atgggttaag gaagagactt
     3421 tagagcattt tttcattcaa gagtgcggaa tgatttgaaa tatggtataa taaaagggaa
     3481 tttctacaga aaagagaaga ttatgtcaaa ttttgccatt attttagcag cgggtaaagg
     3541 gactcgcatg aaatctgatt tgccaaaagt tttgcacaag gttgcgggta tttctatgtt
     3601 ggaacatgtt ttccgtagtg tgggagctat ccaacctgaa aagacagtaa cagttgtagg
     3661 acacaaggca gaattggttg aggaggtctt ggctggacag acagaatttg tgactcaatc
     3721 tgaacagttg ggaactggtc atgcagttat gatgacagaa cctatcttag aaggtttgtc
     3781 aggacacacc ttggtcattg caggagatac tcctttaatc actggtgaaa gcttgaaaaa
     3841 cttgattgat ttccatatca atcataaaaa tgtggccact atcttgactg ctgaaacgga
     3901 taatcctttt ggctatggac gaattgttcg taatgacaat gctgaggttc ttcgtattgt
     3961 tgagcagaag gatgctacag attttgaaaa gcaaatcaag gaaatcaaca ctggaacata
     4021 cgtctttgac aacgagcgtt tgtttgaggc tttgaaaaat atcaatacca ataacgctca
     4081 aggcgaatac tatattacag acgtcattgg tattttccgt gaaactggtg aaaaagttgg
     4141 cgcttatact ttgaaagatt ttgatgaaag tcttggggta aatgaccgtg tggcgcttgc
     4201 gacagctgag tcagttatgc gtcgtcgcat caatcataaa cacatggtca acggtgttag
     4261 ctttgtcaat ccagaagcaa cttatatcga tattgatgtt gagattgctc cggaagttca
     4321 aatcgaagcc aatgttacct tgaaagggca aacgaaaatt ggtgctgaga ctgttttgac
     4381 aaacggtact tatgtagtgg acagcactat cggagcagga gcggtcatta ccaattctat
     4441 gattgaggaa agtagtgttg cagacggtgt gacagtcggt ccttatgctc acattcgtcc
     4501 aaattcaagt ctgggtgccc aagttcatat tggtaacttt gttgaggtga aaggatcttc
     4561 aatcggtgag aataccaagg ctggtcattt gacttatatc ggaaactgtg aagtgggaag
     4621 caacgttaat ttcggtgctg gaactattac agtcaactat gacggcaaaa acaaatacaa
     4681 gacagtcatt ggagacaatg tctttgttgg ttcaaattca accattattg caccagtaga
     4741 acttggtgac aattccctcg ttggtgctgg ttcaactatt actaaagacg tgccagcaga
     4801 tgctattgct attggtcgcg gtcgtcagat caataaagac gaatatgcaa cacgtcttcc
     4861 tcatcatcct aagaaccagt aggagcctat catggagttt gaagaaaaaa cgcttagccg
     4921 aaaagaaatc tatcaaggac caatatttaa actggtccaa gatcaggttg aattaccaga
     4981 aggcaaggga actgcccgac gggatttgat tttccacaat ggggctgtct gtgttttagc
     5041 agtaacggat gaacaaaaac ttatcttggt caagcagtac cgcaaagcta tcgaggctgt
     5101 ctcttacgaa attccagccg gaaaattgga agtaggagaa aacacagccc ctgtggcagc
     5161 tgcccttcgt gaattagagg aagaaacagc ctatacaggg aaattagaac tcttgtacga
     5221 tttttattca gctattggct tttgtaatga gaagttaaaa ctatatttag caagcgattt
     5281 gacaaaagtg gaaaatccgc gtccgcagga tgaggatgaa accttggaag tccttgaagt
     5341 gagcttagaa gaagcgaaag aattaatcca atcaggtcat atctgtgatg ccaagacaat
     5401 tatggctgtt cagtattggg agttgcagaa aaaatagagg aggtcagtat gggtaaatct
     5461 ttattaacgg atgaaatgat tgaaagagct aatagaggcg aaaaaatttc aggtcctcct
     5521 ttgctagatg ataatgagga aactaagatt ttaccaacct cttcttcccg ttttggttat
     5581 gccaatccta aggatcatgg ttttagccag gaaaccttga agattcaggt cgaaccatct
     5641 attcataaaa gccgtcgtat tgaaaatacc aagagaaatg tcttcaattc taagttgaat
     5701 aaaatcttat ttgcggtcat ctttctcttg attttgcttg ttttagcaat gaaacttttg
     5761 taatagaaaa ggaattgaaa tgaaaatagg aattattgct gctatgccag aagaactggc
     5821 ttatctggtc cagcatttag ataataccca ggagcaagtt gttttgggga atacctatca
     5881 tacaggaacc attgcttctc atgaagtcgt tcttgtagaa agtggaattg gtaaggtcat
     5941 gtctgctatg agtgtggcga ttttggctga tcatttccag gtggatgccc ttattaatac
     6001 gggttcagct ggggcagtag cagaaggtat cgctgttggg gatgtcgtga ttgctgacaa
     6061 attagcctat catgacgtgg atgtcacagc ttttggctat gcttatggac aaatggcgca
     6121 acaaccgctt tatttcgaat cagacaaaac ctttgttgct caaatccaag agagtttatc
     6181 tcaattggac caaaactggc atcttggttt gattgctaca ggagatagtt ttgttgcagg
     6241 aaatgacaag atagaagcga ttaagtccca tttcccagaa gttttagccg tggagatgga
     6301 gggggcagct attgctcaag cagcgcatgc cctcaatctc ccagtcttag tcatcagagc
     6361 tatgagtgac aatgccaacc atgaagcaaa catctttttt gatgagttta ttatcgaagc
     6421 tggacgtcgc tctgcccaag tcttgttgac ctttttgaag gctttagatt aagcggaaat
     6481 ttgacagttt ttctagctta tgataagatt taagtaaaga aaagctagaa aacgtttcag
     6541 aggatattat gagtattgaa atgaccgtca gtgagattgc agaggtctta ggattatctc
     6601 gccaagcaat caataaccgt gtcaaagaat taccagaaga agacacagat aaaaatgaca
     6661 aaggggtaac agttgttacc agaagtggct tgattaagct agaagaaatc tataaaaaaa
     6721 cgatttttga agatgagcct gtcagtgaag atgtcaaaca acgtgaactg atggagattc
     6781 tagtggatga gaagaatgca gaaattcttc gtctttatga acaattaaaa gccaaggatc
     6841 gtcagttatc agaaaaagac gagcagatgc gtatcaaaga ccgtcagatt gctgagaaag
     6901 acaaacaatt agatcagcaa caacaattga cccttcaagc catgaaggat caagaaaatc
     6961 ttaagctaga gctggaccaa gcaaaagaag aagtccaatc cactaagaaa ggcttttttg
     7021 ctcgtttatt tggaggataa tcaaggagct gtttaggtta aatgctaacc tgatggcttc
     7081 ttttgtttgt gaatagtata gttgattaga acagagacag tagaggagag gtacagaatg
     7141 gaacgattag aagattacat tgcttttgac ttagaattta atcagcacga aggacaaaca
     7201 cacttgattc aagtatcggc agtgcgtttt agagatggtc gggaaatcga tgcctatgat
     7261 tcctatgttc ataccagcgt ccctctaaaa agctttatca atggtttgac cgggattaca
     7321 gctgagacct taaaagatgc tcctccagtt gagaaagtct tagaagaatt ccaagagttt
     7381 gtgggttctt tgctgatgat aggctacaat gctgctaaaa gtgatttacc tattttagca
     7441 gagcatggtt tagactatag ccaacagtac aaggtggatt tgtatgatga agcttttgaa
     7501 cgtcggagtt cagatttaca tggtattgcc aatctcaaat tgcaaactgt tgcctcattt
     7561 cttggatttc aaggtcagtc tcataatagt ttagaggatg ctcggatgac ggcgcgtgtt
     7621 tacgaatcct tcttagagtc ggatcaagct agggaattgt tagagacaga aagtagctta
     7681 tcaaacaatc cttttggtgt cttggacttg tctcaattat ttgactagga gtgcctatga
     7741 aacctgaaaa acctaaaaaa ttgggtctta aacagattta cctcaatctt gataaaatcc
     7801 tctttctatt tttcttgatt tttatgatag tggagtattt gtgggtacca ttcaattcat
     7861 ggttggctag cttcttatta gatcagacag gttatctttt tatttcctat aacaatgtct
     7921 tagccatcct tactcattct ccattgatta gtctagcttt tttagccttg attgtaatta
     7981 accttctggt tgcttatttt caaataggac tcctctttat tggtgctcgt catctcctct
     8041 ataatgaaaa gcgaaggcta attgagtata tccgcaaggt tttccatgaa agttttggat
     8101 ttatgaaaaa gctaacttgc gctaaagcct tgtttatctt tttctatata gctatgcttt
     8161 ttccttttat acggaaaatg ctgaagattt attatcttaa taaaatcgtc attccagagt
     8221 ttatccaagc ttatttagaa gataaatatt ggatgtggtg gctgagcatc ctacttctat
     8281 ctcttatctt tctctatgtc tctgtcagat tgatgtttgc ccttccgaag attgtatatg
     8341 atcagctgac tgttcgagaa gcagtgatgt ttagcttgga gaaaaccaag aagagagtta
     8401 ctttttatgc ttggaattta ttttacatct tgctcaaagc acatttatta ttttatcttc
     8461 ctttgattcc tttattattg gctcaaactt tggtagatga tatcactcag agagagtctc
     8521 tgattcttgg tattttgaat tttgtcgtga ttaaaaatct ctattatatg gctctgactt
     8581 attttttggt taaatttgtt tcatttttga caggaaagga gctggatatg cttcctagga
     8641 gagaaaaaga tcacatcgtg cgatggggtg tcatgacttg tgccagtctt ttctttgcct
     8701 tagaaggtta tatttatctg gaggctccca tggttcatct acctcaactt atttctcacc
     8761 gaggggtttc caatgcaaat gggattcaaa atacagtaga gtccttggaa actacagcac
     8821 aactcaaacc agacttggtg gagacggacg tgcaggaaac aaaagatggg cagtttgtca
     8881 tgatgcatga tgctaacttg aaaaatctag caggtatcaa taaaagtcct caagacttaa
     8941 acttggagga gcttaaaggg attgatattt ttgaaaatgg ctaccagact aaaatttcaa
     9001 gctttgaaga ttatctcagt cgagccaacg aacttggtca aaaattacta attgaaatta
     9061 aaaccagtaa aaaagatagt ccagacatga tgaaccgctt tttagcccgt tatgctgcaa
     9121 agctcaagat ttatggacat caaatccagt ctttagacta ccatgttgtc gaaaaagtaa
     9181 gacagtatga tgccgaactg ccagtttatt tcatcatgcc ctacaattct gtctttccta
     9241 aaacaagagc gacaggatat actatggagt actcaacctt ggatgaatat tttgtaagca
     9301 aactatggac aacggatcag aaactttatg tctggactgt caatgattca gaagctatca
     9361 gcaaatctct tcacttagga gtagatggag tgattacaga tgatttggaa aaagttcagc
     9421 gggaaataga agtagcccaa gaagatccag aatatacgga tttgctcttg aaaaaagcat
     9481 tggaattctt tgaattttag taataaaaaa gaggaacggt tgttcctccc atgataggta
     9541 agtattcttg tgaatgcttg cttttttgtg gttgcaatcc gtttacgaga attgtttgta
     9601 ttgttcttgt ttcaatttac tttatagtgc tttgaagttg gaatgctaca ctataacttc
     9661 t
//
LOCUS       DAVBHS010000077         9637 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVBHS010000077 DAVBHS010000000
VERSION     DAVBHS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9637
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.077"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(60..659)
                     /locus_tag="VQ651_001310"
     CDS             complement(60..659)
                     /locus_tag="VQ651_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2754310.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(660..1757)
                     /locus_tag="VQ651_001311"
     CDS             complement(660..1757)
                     /locus_tag="VQ651_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2754311.1"
                     /translation="MLERLKSIHYMFWASLIFMIFPILPVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLFWGIMLIYVAGNTAFVAVNYIWFFFFLSNLLSYHFSVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYLLGILTFVDLMTLGSVRIRLVEDLKEAQ
                     TKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQIQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVEIANQLDTASLTQEL
                     ESTASMILLELVTNIIKHAKASKAYLKLERTDQELLLMVRDDGKGFETVTENELHTVR
                     ERVLPFSGEVSVISQKHPTEVQVRLPYKERK"
     gene            complement(1757..2494)
                     /locus_tag="VQ651_001312"
     CDS             complement(1757..2494)
                     /locus_tag="VQ651_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2754312.1"
                     /translation="MKRWIALNKIEFLLTKRQSVYYLLSVGMPTAFYLFFSGMYQDTP
                     GGPANFMRDYLISMTAFSMMSTAIFSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMMVDYLVSILVVFSVGHLVRGVDMSLGSWIGAAFLLIVGSIAFVALGLTLTLLPSS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAIGKCLPTYQLMELLKTFLNEGSINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(2496..3380)
                     /locus_tag="VQ651_001313"
     CDS             complement(2496..3380)
                     /locus_tag="VQ651_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754313.1"
                     /translation="MNMIKVQGLHKNIKSKAILKDISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVYIQDLPVTSSKLDYTRSYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLNNQEIDQLLQFDQQQKEQFAEKLSGGQKRLLSFILTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     EIEKHFILPLAYKEVVEKSVLVENWVQKQDALQVVTCEADAFWELLVQAGCRIQEIEV
                     NNRSLLDTIFEETQKGDD"
     gene            complement(3367..3561)
                     /locus_tag="VQ651_001314"
     CDS             complement(3367..3561)
                     /locus_tag="VQ651_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754314.1"
                     /translation="MKKAILFLFIGLSLVVWLVEMFTGWFDQSFLHQVIRGAWGLGFM
                     ISVVFPMGMEWLKGEYYEHD"
     gene            complement(3558..3764)
                     /locus_tag="VQ651_001315"
     CDS             complement(3558..3764)
                     /locus_tag="VQ651_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754315.1"
                     /translation="MEEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEGFYHFPWELFAS
                     FGIMSWLIREGLKLVRDVKKELEK"
     gene            complement(4249..4322)
                     /locus_tag="VQ651_001316"
     tRNA            complement(4249..4322)
                     /locus_tag="VQ651_001316"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4286..4288),aa:Pro,seq:tgg)
     gene            complement(4338..4411)
                     /locus_tag="VQ651_001317"
     tRNA            complement(4338..4411)
                     /locus_tag="VQ651_001317"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4375..4377),aa:Arg,seq:acg)
     gene            complement(4421..4506)
                     /locus_tag="VQ651_001318"
     tRNA            complement(4421..4506)
                     /locus_tag="VQ651_001318"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4470..4472),aa:Leu,seq:taa)
     gene            complement(4514..4585)
                     /locus_tag="VQ651_001319"
     tRNA            complement(4514..4585)
                     /locus_tag="VQ651_001319"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4551..4553),aa:Gly,seq:gcc)
     gene            complement(4608..4680)
                     /locus_tag="VQ651_001320"
     tRNA            complement(4608..4680)
                     /locus_tag="VQ651_001320"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4645..4647),aa:Thr,seq:tgt)
     gene            complement(4699..4780)
                     /locus_tag="VQ651_001321"
     tRNA            complement(4699..4780)
                     /locus_tag="VQ651_001321"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4744..4746),aa:Leu,seq:tag)
     gene            complement(4786..4858)
                     /locus_tag="VQ651_001322"
     tRNA            complement(4786..4858)
                     /locus_tag="VQ651_001322"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4823..4825),aa:Lys,seq:ttt)
     gene            complement(4883..4955)
                     /locus_tag="VQ651_001323"
     tRNA            complement(4883..4955)
                     /locus_tag="VQ651_001323"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4920..4922),aa:Asp,seq:gtc)
     gene            complement(4958..5030)
                     /locus_tag="VQ651_001324"
     tRNA            complement(4958..5030)
                     /locus_tag="VQ651_001324"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4995..4997),aa:Val,seq:tac)
     gene            complement(5035..5150)
                     /gene="rrf"
                     /locus_tag="VQ651_001325"
     rRNA            complement(5035..5150)
                     /gene="rrf"
                     /locus_tag="VQ651_001325"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(5227..8128)
                     /locus_tag="VQ651_001326"
     rRNA            complement(5227..8128)
                     /locus_tag="VQ651_001326"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(8253..8325)
                     /locus_tag="VQ651_001327"
     tRNA            complement(8253..8325)
                     /locus_tag="VQ651_001327"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8290..8292),aa:Ala,seq:tgc)
     gene            complement(8377..>9637)
                     /locus_tag="VQ651_001328"
     rRNA            complement(8377..>9637)
                     /locus_tag="VQ651_001328"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2562 a         2540 c         1848 g         2687 t
ORIGIN      
        1 tattgttcca ttcactaccg tcaattttag cacatagtct tcatgaaaat attatatcat
       61 cataaccaac cagattcttt tgcgatatta gctgcctctg ttcgattacc agcatccagt
      121 tttgaaagaa tattggtgac atagtttcga atagttccgt ttgagaggta aagctgatct
      181 gcgatttctt ggttggataa gccccgggcg attcccttta agacagcaat ctcttgttct
      241 gtcaacggat tgggacgggt catcaccact tccatcaatt caggcgaata ctccttgcgt
      301 ccttcgagga cggtgtgcaa ggtttgcatg aggtctgcaa tgcttcgttc ctttaataca
      361 taagcatcca ctccagcctt gaccgcacgt tcaaaatacc cagcacgctt gaaggtcgtc
      421 accacaacca cctttgtttc aagcctttct gatcgtatcc actccaagac ttcaagacct
      481 gtcttaacag gcatttctac gtcaaggatg gcgatatcta cagactcctt ttctaatagt
      541 tggattgctt cttgcccatt cttggcttga aggacagact ccacatctgg ttgaagcgtg
      601 agcaattgac acatagcatc tcgcaacata ctttgatctt ctgcgactaa tactttcatc
      661 tactttctct ccttataagg tagtcgaact tgcacttccg ttggatgttt ctgactgatt
      721 acacttactt ctcctgaaaa tggaaggaca cgttctcgaa ctgtatggag ctcattttct
      781 gttacagttt caaaaccttt cccgtcatct ctgaccatca ggagcaattc ctgatctgtg
      841 cgttctaatt ttaagtaggc tttagacgct ttagcatgtt tgatgatgtt ggtcactaat
      901 tcaagcaaaa tcatggaagc cgttgactct aattcctgag ttaagctagc tgtatctagt
      961 tgattagcta tttccacctc aattccagca atttctaaca tctttttcac agtctctagt
     1021 tcggatgtca aagttctaga cttaagattt tccacaatgg ttcgcacttc acgcattgat
     1081 tctttgctaa tctgctgtat ttctcttaat tccttttcca cttgtggata agcctgtatt
     1141 tgaaataact gcaaggctag atcagtttta acactaagca tagcaaaggt atgccccaga
     1201 ctatcatgca aatcttgacc gatacgattg cgctcatttt ctgctagcaa tagatttatc
     1261 tgggtatttt gcttagtctg agcttctttc aaatcctcga caaggcgaat tcgaaccgag
     1321 cccaaagtca tcaaatcaac aaaagtaaga attccaagca gatagactag aaactcaact
     1381 tcgattctct gaaaaatcaa aagtcgcccc acaacaagga cttgagcaag aagaaaagtc
     1441 cagacatgta aagactttaa actacgtacg ctgaaatgat aacttaagag attggaaagg
     1501 aaaaagaaaa accagatata attcacagca acaaaggcgg tattcccagc tacatagatc
     1561 agcatgatcc cccagaaaag ccaagatagg cgctggctct tagttgttaa aacacccaaa
     1621 tatgccacta caaatagaat atcaatcaat aaatgccagg cagaaagcca cccagtcact
     1681 acaggtagga tggggaaaat cataaaaatt aaactggccc aaaacatata gtgtatgctt
     1741 ttcagtcttt caagcattaa gcattctcct tatgaccttg aaggtaaatg gtcaaaccaa
     1801 acaaaactgc tgaaaaaaca agtagataaa ctgtggctga tagattgatg ctaccctcgt
     1861 ttaagaaggt cttgagcaac tccatcaact gataagttgg taggcacttc ccgattgctt
     1921 gcatccagtc tggaaataaa gagatgggca tccagagtcc acctaaaaca gccaagccta
     1981 gatagagaag attgcccacg acagacatca gctgactaga aggcaagagt gtcaaggtca
     2041 agccaagcgc tacaaaagct atacttccta ctatcagcaa gaacgcagcc ccaatccagc
     2101 ttcctaaaga catatccaca cctctgacca agtgcccaac tgagaaaaca accaggattg
     2161 agaccaaata atcaaccatc atacttgtta tctttgatag ataatattct accatattta
     2221 cagggctatg acgcaatgtt ttctgccagt tgttcatctt atcggtatgt aagacagctg
     2281 ggaatgaaaa gatagcggtc gacatcatgg aaaaggcagt catggagatg aggtagtcgc
     2341 gcataaaatt agccggtcca cctggtgtgt cctggtacat gcctgaaaag aatagataga
     2401 aggctgtcgg catccctaca gataataaat aatagactga ttgccgtttg gtcaatagaa
     2461 attctatctt gtttagtgca atccatcgtt tcatcttagt catctccctt ctgtgtttct
     2521 tcaaagattg tatccaacaa actacgatta ttgacttcga tttcttgtat cctacatcct
     2581 gcttgaacta acagttccca gaaagcatcc gcttcgcatg tgactacttg taaggcatct
     2641 tgtttttgta cccagttttc aaccaagact gacttctcaa cgacttcctt gtaagctagc
     2701 ggaaggataa aatgcttttc aatctcctca ctacgcatag ctagaggcgt cgtgtcacga
     2761 atcaactctc ccttatttaa aaccaaaatc cggtcagctg tatgctctac ctcttcaata
     2821 taatgagacg aatagagaat ggtgactcct tgcgctttta ggtcccgaac aatttcccaa
     2881 aaacgttgac gagttgaagt atccatggca gcagttggct catctaaaaa gacaagcttt
     2941 ggtcgcccaa ttaaggtcaa gataaaagat aaaagacgct tttgcccacc tgacaatttt
     3001 tctgcgaatt gctctttctg ttgctggtca aactgtaata gttgatcgat ttcctggttg
     3061 ttcaagggat ttggatagat acgttgaaag aaagcaatca actctttaac ctttagtttt
     3121 tgaacaatta cattttcttg agggagatag gatctcgtat agtctaactt agagctcgtt
     3181 accggcaaat cttggatata tacttgccca ctggtgacca atttatctcc aagcaaacag
     3241 tccaagagtg tcgtcttacc agctccattt ggcccaatca aggcgacgca ttcaccttca
     3301 gctacctcaa aggaaatatc cttcaaaata gccttacttt tgatgttttt atgtaagcct
     3361 tgtaccttaa tcatgttcat aatattctcc tttcaaccac tccattccca tagggaaaac
     3421 gacggaaatc ataaatccta atccccaggc gccgcgaata acttggtgaa gaaaggattg
     3481 atcaaaccaa cctgtaaaca tttctaccaa ccataccact agtgatagtc caataaaaag
     3541 aaagagaatc gcttttttca tttttcaagc tcctttttca catctcttac taatttcaag
     3601 ccttctcgta tcaaccaaga catgattcca aagcttgcaa acaactccca aggaaaatga
     3661 tagaatcctt cgtccaatcc tgagaacatg agataagtca tgactcctgc tactactaaa
     3721 ctcactgcga taatcatttt atttctcatc tcttcttcct ccatttcata ctacaattat
     3781 agtcttttga aatcagagga gatagaagct tctgtcacta aaaaatatga caaatgtcat
     3841 aaaaaattct gttcaaaaca agcaagatac actatacaat aaaacacaat tagataaatc
     3901 taaggcaact tcctcaaaag agatatcaaa ccccattcac actataatgt aaactaatac
     3961 ttatttaaag tcaaaagagc ggaaattttt atcgtacaaa catatatatt ataggtaaga
     4021 caaaaaactc ttcaattact ttcttaaaaa tttataggag ttataaaatc tattaaattt
     4081 caatgtggat acctaaacta cagtatttat tgaaaagact ggagacaaaa agtatacgct
     4141 gccaaaatga attgctgaaa atcaaaaaag agagaagcaa actgattctc tcttaatgta
     4201 tataatatct agttataaaa atacacactc acatatctct gtaatgaatc gggaagacag
     4261 gattcgaacc tgcgacacct tggtcccaaa ccaagtactc taccaagctg agctacttcc
     4321 cgagttaaat agaaaaatgc accctagagg agtcgaacct ctaaccgcct gattcgtagt
     4381 caggtactct atccagttga gctaagggtg ctccatatta tgccgaggac cggaatcgaa
     4441 ccggtacgat cgttaccaat cgcaggattt taagtcctgt gcgtctgcca gttccgccac
     4501 cccggcctct ctaagcgaac gacgggattc gaacccgcga cccccacctt ggcaaggtgg
     4561 tgttctacca ctgaactacg ttcgcactgt tttcttctat ctaaaaatgc cggctacatg
     4621 acttgaacac gcgaccctct gattacaaat cagatgctct accaactgag ctaagccggc
     4681 tcatttgtta tatcttaatg cgggttaagg gacttgaacc cccacgccgt taagcgccag
     4741 atcctaaatc tggtgcgtct gccaattccg ccaaacccgc atatatgacc cgtactgggc
     4801 tcgaaccagt gacccattga ttaaaagtca attgctctac caactgagct aacgagtcta
     4861 aaataacttg cgttacctta aacggtcccg acgggaatcg aacccgcgat cttcgccgtg
     4921 acagggcgac gtgataaccg ctacactacg ggacctttgg gagttaacgg gatcgaaccg
     4981 ctgaccctct gcttgtaagg cagatgctct cccagctgag ctaaactccc tagagctaag
     5041 cgacttccct atctcacagg gggcaacccc caactacttc cggcgttcta gggcttaact
     5101 tctgtgttcg gcatgggtac aggtgtatct cctaggctat cgtcacttaa ctctgagtaa
     5161 tacctactca aaatcgaata tctattcaat tcaagaaaac cgttcgcttt catattctca
     5221 gttactttgg ataagtcctc gagctattag tattagtccg ctacatgtgt cgccacactt
     5281 ccacctctaa cctatctacc tgatcatctc tcagggctct tactgatata taatcatggg
     5341 aaatctcatc ttgaggtggg tttcacactt agatgctttc agcgtttatc ccttccctac
     5401 atagctaccc agcgatgcct ttggcaagac aactggtaca ccagcggtaa gtccactctg
     5461 gtcctctcgt actaggagca gatcctctca aatttcctac gcccgcgacg gatagggacc
     5521 gaactgtctc acgacgttct gaacccagct cgcgtgccgc tttaatgggc gaacagccca
     5581 acccttggga ccgactacag ccccaggatg cgacgagccg acatcgaggt gccaaacctc
     5641 cccgtcgatg tgaactcttg ggggagataa gcctgttatc cccagggtag cttttatccg
     5701 ttgagcgatg gcccttccat acggaaccac cggatcacta agcccgactt tcgtccctgc
     5761 tcgagttgta gctctcgcag tcaagctccc ttataccttt acactctgcg aatgatttcc
     5821 aaccattctg agggaacctt tgggcgcctc cgttaccttt taggaggcga ccgccccagt
     5881 caaactgccc gtcagacact gtctccgata gggatcacct atctgggtta gagtggccat
     5941 aacacaaggg tagtatccca acagcgtctc cttcgaaact ggcgtcccga tctcttagac
     6001 tcctacctat cctgtacatg tggtacagac actcaatatc aaactgcagt aaagctccat
     6061 ggggtctttc cgtcctgtcg cgggtaacct gcatcttcac aggtactaaa atttcaccga
     6121 gtctctcgtt gagacagtgc ccaaatcatt acgcctttcg tgcgggtcgg aacttacccg
     6181 acaaggaatt tcgctacctt aggaccgtta tagttacggc cgccgtttac tggggcttca
     6241 attcatacct tcgcttacgc taagcactcc tcttaacctt ccagcaccgg gcaggcgtca
     6301 ccccctatac atcatcttac gatttagcag agagctgtgt ttttgataaa cagttgcttg
     6361 ggcctattca ctgcggctga cttaaagtca gcaccccttc tcccgaagtt acggggtcat
     6421 tttgccgagt tccttaacga gagttctctc gctcacctga ggctactcgc ctcgactacc
     6481 tgtgtcggtt tgcggtacgg gtagagtatg tttaaacgct agaagctttt cttggcagtg
     6541 tgacgtcact aacttcgcta ctaaacttcg ctccccatca cagctcaatg ttacagatat
     6601 aagcatttga ctcatatcac acctcactgc ttagacagac tcttccaatc gtctgcttta
     6661 gttagcctac tgcgtccctc catcactaca tactctagta caggaatatc aacctgttgt
     6721 ccatcggata cacctttcgg tctctcctta ggtcccgact aacccagggc ggacgagcct
     6781 tcccctggaa accttagtct tacggtggac aggattctca cctgtctttc gctactcata
     6841 ccggcattct cacttctatg cgttccagca ctcctcacgg tataccttca tcacacatag
     6901 aacgctctcc taccatacct ataaaggtat ccacagcttc ggtaaattgt tttagccccg
     6961 gtacattttc ggcgcagggt cactcgacta gtgagctatt acgcactctt tgaatgaata
     7021 gctgcttcta agctaacatc ctagttgtct gtgcaacccc acatcctttt ccacttaaca
     7081 attattttgg gaccttagct ggtggtctgg gctgtttccc tttcgactac ggatcttagc
     7141 actcgcagtc tgactgccga ccataattca ttggcattcg gagtttatct gagattggta
     7201 atccgggatg gacccctcac ccaaacagtg ctctacctcc aagaatctct aatgtcgacg
     7261 ctagccctaa agctatttcg gagagaacca gctatctcca agttcgtttg gaatttctcc
     7321 gctacccaca agtcatccaa gcacttttca acgtgccctg gttcggtcct ccagtgcgtc
     7381 ttaccgcacc ttcaacctgc tcatgggtag gtcacatggt ttcgggtcta cgtcatgata
     7441 ctaaggcgcc ctattcagac tcggtttccc tacggctccg tctcttcaac ttaacctcgc
     7501 atcataacgt aactcgccgg ttcattctac aaaaggcacg ctctcaccca ttaacgggct
     7561 cgaacttgtt gtaggcacac ggtttcaggt tctatttcac tcccctcccg gggtgctttt
     7621 cacctttccc tcacggtact ggttcactat cggtcactag ggagtattta gggttgggag
     7681 atggtcctcc cagattccga cgggatttca cgtgtcccgc cgtactcagg atactgctag
     7741 gtacaaagac tattttaaat acgaggctat tactctcttt ggctgatctt cccaaatcat
     7801 tcttctataa tctttgagtc cacattgcag tcctacaacc ccgaagagta aactcttcgg
     7861 tttgccctcc tgccgtttcg ctcgccgcta ctaaggcaat cgcttttgct ttctcttcct
     7921 gcagctactt agatgtttca gttcactgcg tcttcctcct cacatcctta acagatgtgg
     7981 gtaacaggta ctacctgttg ggttccccca ttcggaaatc cctggatcat cgcttactta
     8041 cagctaccca aggcatatcg tcgtttgtca cgtccttcgt cggctcctag tgccaaggca
     8101 tccaccgtgc gcccttatta acttaacctt attttctgac ctttcagtca taaactctta
     8161 ttaatactac agcgttttcg gtttattttc ttgttactat ttgatataga tattcaattt
     8221 tcaatgtgca ttacttggtg atctctcacc aatggagcct agcgggatcg aaccgctgac
     8281 ctcctgcgtg caaagcaggc gctctcccag ctgagctaag gccccacaag acctctcaag
     8341 actaaacaag accaatgcgc agttccttat ccttagaaag gaggtgatcc agccgcacct
     8401 tccgatacgg ctaccttgtt acgacttcac cccaatcatc tatcccacct taggcggctg
     8461 gctccttacg gttacctcac cgacttcggg tgttacaaac tctcgtggtg tgacgggcgg
     8521 tgtgtacaag gcccgggaac gtattcaccg cggcgtgctg atccgcgatt actagcgatt
     8581 ccgacttcat gtaggcgagt tgcagcctac aatccgaact gagactggct ttaagagatt
     8641 agcttgccgt caccggcttg cgactcgttg taccagccat tgtagcacgt gtgtagccca
     8701 ggtcataagg ggcatgatga tttgacgtca tccccacctt cctccggttt attaccggca
     8761 gtctcgctag agtgcccaac taaatgatgg caactaacaa taggggttgc gctcgttgcg
     8821 ggacttaacc caacatctca cgacacgagc tgacgacaac catgcaccac ctgtcacctc
     8881 tgtcccgaag gaaaactcta tctctagagc ggtcagaggg atgtcaagac ctggtaaggt
     8941 tcttcgcgtt gcttcgaatt aaaccacatg ctccaccgct tgtgcgggcc cccgtcaatt
     9001 cctttgagtt tcaaccttgc ggtcgtactc cccaggcgga gtgcttaatg cgttagctac
     9061 ggcactaaac cccggaaagg gtctaacacc tagcactcat cgtttacagc gtggactacc
     9121 agggtatcta atcctgtttg ctccccacgc tttcgagcct cagcgtcagt tacaagccag
     9181 agagccgctt tcgccaccgg tgttcctcca tatatctacg catttcaccg ctacacatgg
     9241 aattccactc tcccctcttg cactcaagtt aaacagtttc caaagcctac tatggttaag
     9301 ccacagcctt taacttcaga cttatctaac cgcctgcgct cgctttacgc ccaataaatc
     9361 cggacaacgc tcgggaccta cgtattaccg cggctgctgg cacgtagtta gccgtccctt
     9421 tctggtaaga taccgtcaca gtgtgaactt tccactctca cactcgttct tctcttacaa
     9481 cagagcttta cgatccgaaa accttcttca ctcacgcggc gttgctcggt cagacttccg
     9541 tccattgccg aagattccct actgctgcct cccgtaggag tctgggccgt gtctcagtcc
     9601 cagtgtggcc gatcaccctc tcaggtcggc tatgtat
//
LOCUS       DAVBHS010000078         9544 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVBHS010000078 DAVBHS010000000
VERSION     DAVBHS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9544
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.078"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            194..454
                     /locus_tag="VQ651_001329"
     CDS             194..454
                     /locus_tag="VQ651_001329"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2754316.1"
                     /translation="MSGGETKRVALAKIILKNPPLIFADELTASLDSTTSQGIMKLLL
                     GMKNKNRLIIIATHNPDIWNQADEVINLNQLLQSIYIFYILK"
     gene            complement(558..1028)
                     /locus_tag="VQ651_001330"
     CDS             complement(558..1028)
                     /locus_tag="VQ651_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2754317.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQTLLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(1045..3318)
                     /locus_tag="VQ651_001331"
     CDS             complement(1045..3318)
                     /locus_tag="VQ651_001331"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HET2754318.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLASDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            3665..6766
                     /locus_tag="VQ651_001332"
     CDS             3665..6766
                     /locus_tag="VQ651_001332"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET2754319.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEVREGAFYYIKGKVQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSDELEAKLNEIVMKT
                     IYR"
     gene            6848..7855
                     /gene="pfkA"
                     /locus_tag="VQ651_001333"
     CDS             6848..7855
                     /gene="pfkA"
                     /locus_tag="VQ651_001333"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET2754320.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            7912..9417
                     /gene="pyk"
                     /locus_tag="VQ651_001334"
     CDS             7912..9417
                     /gene="pyk"
                     /locus_tag="VQ651_001334"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET2754321.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
BASE COUNT         2943 a         1653 c         2102 g         2846 t
ORIGIN      
        1 ccctttttta ttgacagtat caggatatct ttttcaaatt tttggtttgt tagataatga
       61 aaatgtttct atcaatctag atttaggatt aataaatcgt aaatgtaatt ataaagaaaa
      121 agataagtta aaaaaagatg tattagcaag agttggtcta actcatattc atctataaca
      181 aaagatatat gaattgtctg gcggtgaaac aaaacgtgtt gctttagcga aaatcattct
      241 taaaaatcca ccattaatat tcgcggatga gttgactgca tcactcgatt cgacgacttc
      301 acaaggaatt atgaaattgt tattaggtat gaaaaataaa aatcgtctaa tcatcattgc
      361 aactcataat cccgatattt ggaatcaagc agatgaagtt ataaatttaa atcaattatt
      421 acagtcaata tatatcttct atattttaaa gtaattttat ctgaatccgt taagagacta
      481 ttgtcccagg ctctttctga tgttaaagac tttctaccag gttttttaaa agcataattg
      541 ttagttgtag tcatttatta ttcttcaaag aaaaatggtg gggcgaattt tttcagttct
      601 tcaaagcact tttgagcagt atctgcatct tcacagatga taagacagac atcattacca
      661 caaagggtag cgatagcgtc agggaagctc aaagtatcaa tgatagaacc aaaggattga
      721 gccagtccag gaagggtttt tagtaggact tggtgttgaa ctgggcgcat ccagacaagg
      781 gcgtcttcca tgtagagttc gagacgtttt tcccattttg agatggaacc attgttaaga
      841 acataataag cgctatcttc ttcgcggact tttgataggt tcatattttt gatgtcgcgt
      901 gagagggttg cctgagttac ttgaatgtcg ttctcagcaa gaagggtttg caactcagcc
      961 tgtgtatgaa tcttgttttt tgtgataaga gcgcgtataa gttggtggcg gtgttctgat
     1021 ttattcataa taatgtaact ccttttagca aggtaaggta agcatggact gagcgaggtc
     1081 gacagtcaag tggtagtctg tattgtcacg gatggtgatt tcaaagtcag tagtatagag
     1141 gactaaacgg agagtgtctc cttcttttag cttgtaaata gttggctgca gttcaaattg
     1201 aacgtccatc cattcatctg cagtaatatc ctctactaac agtaaatcat ttctattttg
     1261 taaattaagg taaccttttg tcacgactct ttgtgcctct ggtctaaatg gcaattcaca
     1321 gagattttcc aacatgtgat agcgaccgtt gtcaatggtt ctagcactta aaatagctgg
     1381 ataaggttgt aggtatttct tttgcccaaa ttctagcagt tgggcagata agagcccctt
     1441 gtttgtactg gatttgatac gaagattgag ctgagcgcga ccgtttaggt ggagatcttt
     1501 agtcacagga aggttaatag taatctgatt agctttccct tgatagagct ctgtattgaa
     1561 ggtttggtat gtcttaccat agcgctcaaa atccttatct gggtactggt tttgaatagc
     1621 ttgctcttct tgaccaagtg agaaggtttc acagttttct tgcccaccga agttatcaag
     1681 tgataaccaa gtctgtggag ctgtattgtc ctgccagata acagtaggaa gttgaaagtc
     1741 tgtttcctgt cctagtaatt tcttggtcaa taaggcattt atggactcac ggaagtcaat
     1801 tgattgccaa ttgttcatgt aaacatgggc accattatgg aaaaagagat gcttgtgtat
     1861 atgagtagga agagcatgga acatctggta aacatgaagt ggtttgacat tccaatcctg
     1921 agaaccatga gtaaagacaa cctctgcctt tactttatgg gcattgagca gataattgcg
     1981 gtcatgccaa aactgattgt agtccccagt ttttcggtct agttgagctt tcactttttc
     2041 taagtcagct tggtgagctt cattgccacg gatatagtcg ctagccaaga gattacgaga
     2101 ataggttaac tcagcaaggg agtcaaagtc ctcacctgga taaccacctg ggctagtcac
     2161 cagaccgttt tcacggtagt agttgtacca tgatgaaatt cctgcctcgg caatgataac
     2221 ttctaaacca tcgactcctg tagtcgcaag accattggac atggtaccta gataggaaag
     2281 tcccgttgta gcaacttttc cgtttgacca atcagccttg acttgacgct ggcgcgtgtg
     2341 atcagtaaag gcacggcaac ggccgttaag ccaatcgatg acatttttat aagcctcgat
     2401 ttgctggtag tctccattag tcatgaaacc tgtcgagtct ttggtaccaa cacctgagac
     2461 atagagattg gcaaagcctc tcggaaggaa gtagtcgttt agtgtatagc tagagttgat
     2521 gtgagttagc ttttcctcag cctctgctat aagctcagct ttaccttggg gttggacgag
     2581 atttagttga ggtttctcta gctcaatctt gtgaggaagt ttaacctcaa gctcgccctc
     2641 catcttgtag agagccttgt cactagcctt gtcattggtt ccctgatgat aagggctggc
     2701 tgtcatgatg gcagggattt ttccatcaaa acgagggcga ataatgctaa cctttactag
     2761 gtctgatagc cctttttggt cagtatcgac acgagactca acgtaaacga cttcacgaat
     2821 gacatcctgg ttagaaaaag tagccaaact cttgccgtta aagtagtggt agtcattatc
     2881 ctccggaata agaccatcac taacaagttg gtcgataaga gtatttcctt ttttggtgcg
     2941 agtattgagt aactgataga gattttcaat caagtcacca tatataatgg gaaatccagt
     3001 ttctttacga aaaacgtcac tatcttcgaa gtcaaccaaa taagaaaagc ctaaaagttg
     3061 aaaagcaaca gtataaaaaa tatctgctgt cagttcatct tctgattgaa aaaatgtcag
     3121 caggtctgtt tttttatcag ctgctaggat agaaagtggg tagttggtgt cttgataagt
     3181 gaaaaagaaa cgacgtaaaa aggtttcaag tgagtctttg tgattggctg tattttgtaa
     3241 atcaaagcca cattttttta gttcagataa gacattttct tttggaaaat tgatataact
     3301 atattgatta aaacgcatag aacctccata tagaatgaca gttaaggtta ttatatcaaa
     3361 aaaaaagcag aaagggaatt gttaacttca aaaggaaata atccaataaa aatgaataaa
     3421 gtactaaatt caatatagag aacagagtaa caataagaat aaatagatag ggtataaaag
     3481 ttctaggaga tttatattat atgctttctg tttttatata caatatagta taaatataaa
     3541 aatgatgaca aaaatacaaa tgaatagaaa ataaattagt aagctgatga aatttttctc
     3601 aagagaagcc atttataggt gaaaatggta taatatagtg agaaggatag aggagaagtg
     3661 taaattgatc gcacaactag atacaaaaac agtctatagt tttatggaaa gcgtcatttc
     3721 gatcgaaaag tatgtgagag cagctaaaga atacggctac actcatttgg ctatgatgga
     3781 tattgacaat ctttatggcg ctttcgactt tctagagatt acaaaaaaat acggcattca
     3841 tcctttgcta gggcttgaaa tgacagtgtt tgtagatgat cagggagtga atttgcgctt
     3901 tttagctcta tctagtgtgg gctatcagca gttgatgaag ctttcgacag ccaagatgca
     3961 gggggagaaa acttggtcag tcctgtccca gtacctggag gatatcgcgg tcattgtgcc
     4021 ttattttgat agagttgagt cgttagaact aggctgtgat tactatatag gggtttatcc
     4081 agaaacacta gcaagcgaat ttcatcatcc tatcttacct ctttatcggg tcaacgcttt
     4141 tgaaagcagg gatagagaag ttcttcaagt tttaacagcg attaaagaaa atctaccgct
     4201 cagagaagtt cccttgcgtt cgagacaaga tgtctttata tcagcaagtt ctttagagaa
     4261 actattccaa gagcgttttc cgcaagcttt ggacaattta gaaaagctta tttcaggcat
     4321 ttcttacgac ttggatacta gtctgaaact gcctcgtttt aatccagcta gaccagcagt
     4381 agaggagttg agagagcgtg ctgaactggg gcttgttcag aaggggttga ctagtaaaga
     4441 atatcaagat agactagacc aagaattgtc tgttattcat gatatgggct ttgatgatta
     4501 tttcttggtt gtttgggatt tgttgcgttt tggacgatcg aatggctatt atatgggaat
     4561 gggaaggggt tctgcagtag gcagtttggt ttcttatgcc ttagacatca cggggattga
     4621 cccagtagag aaaaatctga tttttgaacg ctttcttaat cgtgaacgct ataccatgcc
     4681 tgatattgat attgatatcc cagatattta tcgtccagat tttatcagat atgttggtaa
     4741 taaatatggt agtaaacatg cggcacaaat cgttactttt tcaacctttg gagccaagca
     4801 agctcttcga gatgtcttga aacgctttgg tgtgccagag tatgaattat ctgcaattac
     4861 taagaaaatc agttttcgtg acaatcttaa gtcggcctat gagggcaatc tccagtttcg
     4921 tcagcaaatc aatagtaagt tagaatacca aaaagctttt gagattgctt gcaagataga
     4981 gggctatcca aggcaaacct ctgtccatgc ggctggtgtt gtaattagtg accaagattt
     5041 aaccaactac attcctctaa agtatggtga tgaaattcca ctgactcagt atgatgctca
     5101 tggagttgag gctagcggac ttttgaagat ggactttctg ggactacgaa atttgacctt
     5161 tgtccagaag atgcaagagt tgcttgctga aacagaaggt attcatctga aaattgaaga
     5221 aatcgattta gaagacaaag aaacattagc tttatttgcc tctggtaata caaaaggtat
     5281 ctttcaattt gagcaaccag gtgccattcg tctgcttaag cgtgtgcaac cagtctgttt
     5341 tgaagatgtc gtcgcgacta cttctctaaa tcgaccgggt gctagtgact atatcaataa
     5401 ttttgtggca agaaagcatg ggcaggaaga agtgactgtt ctggatccag tactggagga
     5461 tattttggct ccaacctacg gcataatgct ctatcaggag caggttatgc aggttgccca
     5521 gcgacttgcc ggatttagtc ttgggaaagc cgatattttg cgtcgggcta tggggaaaaa
     5581 ggatgcctct gccatgcatg agatgagggc ttcctttatt caaggttcat tagaagctgg
     5641 tcatactgtg gaaaaagcag agcaggtctt tgatgttatg gagaagtttg caggttatgg
     5701 ttttaacagg tcacacgcct atgcctactc agccttggcc ttccagttgg cttatttcaa
     5761 aacgcattat ccagccattt tttatcaggt catgttaaat tcttccaaca gtgattactt
     5821 aatagatgca cttgaagcag gttttgaagt agcctctcta tccatcaaca ccattcccta
     5881 tcacgataaa attgccaaca aggccatcta tctaggtttg aaatccatta aaggagtcag
     5941 taatgattta gctctctgga ttattgaaaa tagaccttat tctaacattg aagattttat
     6001 agctaaatta cctgagaatt atctgaaact tcctctgcta gaacctttgg taaaagttgg
     6061 tcttttcgat tcatttgaaa aaaatcgtca aaaagtattt aataacttag ctaatctatt
     6121 tgaatttgtg aaagagttgg gaagtttgtt tggagatgct atttatagtt ggcaggaatc
     6181 ggaagattgg acggaacaag aaaaatttta tatggaacaa gagcttttag ggataggtgt
     6241 cagcaaacat ccactacaag ctattgcaag taaggctatt tacccgatta ccccaatcgg
     6301 aaatttgtca gaaaatagct atgctattat cttggttgaa gttcagaaaa taaaagtgat
     6361 tcgtaccaaa aagggtgaaa atatggcctt cttacaggca gatgatagta agaaaaaatt
     6421 ggatgttaca ctcttctcag atttatatcg acaggttggt caggaagtaa gagaaggagc
     6481 attctactat atcaaaggga aagtccaatc acgtgacggt cgtctgcaaa tgattgcaca
     6541 agaaataaga gaagcagttg ctgaacgctt ttggatacag gtgaaaaatc atgaatcgga
     6601 tcaagaaatt tcacgtattt tagaacaatt taaaggccca atcccagtca tcatccggta
     6661 tgaagaggaa cagaaaacca tcgtttctcc ccatcatttt gtagctaaat ctgatgaatt
     6721 agaggcgaaa ttgaatgaaa tcgttatgaa aacgatttat cgctaaaaat acggaaaata
     6781 gaagaatttt aaaatcaaat gtgatataat caagaagaat gttaaaagaa aaaggagcat
     6841 aaccaatatg aaacgtattg ctgttttgac tagtggtgga gacgcccctg gtatgaacgc
     6901 tgccatccgt gcagttgttc gtcaagcaat ttcagaagga atggaagtgt ttggtatcta
     6961 tgacggatat gctggtatgg ttgccggtga aattcatccc ctagatgcag cttcagtagg
     7021 ggacatcatt tctcgtggtg gtactttcct tcactcagct cgttacccag agttcgctca
     7081 acttgaaggg caacttaaag ggattgagca attgaaaaaa cacggaattg aaggtgtagt
     7141 tgttatcggt ggtgacggat cttaccacgg cgctatgcgt ttgactgaac atggcttccc
     7201 agctattggt cttccaggta caatcgataa cgatatcgtt ggtactgact ttacaatcgg
     7261 ttttgacaca gcggttacta ctgccatgga cgctatcgat aagattcgtg atacatcatc
     7321 aagtcaccgt cgtacttttg taatcgaagt tatgggacgt aacgctggtg atatcgctct
     7381 ttgggctggt attgcaactg gtgctgatga aatcatcatc cctgaagcag gcttcaagat
     7441 ggaagatatc gtagcaagca tcaaagctgg ttatgaatgt ggtaaaaaac acaatatcat
     7501 cgtcttagct gaaggtgtga tgtcagcggc tgaatttggt caaaaactta aagaagctgg
     7561 agatacaagc gaccttcgtg taacagaact tggacatatt caacgtggtg gttctccaac
     7621 tgcgcgtgac cgtgttttgg cgtcacgtat gggtgcacat gctgttaaac ttcttaaaga
     7681 aggtatcggt ggtgttgcgg ttggtattcg taacgaaaaa atggttgaaa atccaattct
     7741 tggtactgca gaagaagggg cattgtttag ccttactgca gaaggtaaga ttgtggttaa
     7801 caacccgcac aaagccgata ttgagctatc tagcttgaat aagagcttgt cataatttac
     7861 aatttagtta ataagaccaa aaaggtcata tataaaggag tcacaaaaat catgaacaaa
     7921 cgtgtaaaga tcgttgcaac tttgggtcct gcggtagaaa tccgtggtgg taaaaaattc
     7981 ggtgaggacg gatactgggg tgaaaaactt gatgttgaag cttcagctaa aaacattgct
     8041 aaattgattg aagctggtgc taacacattc cgattcaact tctcacacgg cgaccaccaa
     8101 gaacaaggtg agcgtatggc aactgttaaa cttgcggaaa aaattgcagg taaaaaagtt
     8161 ggtttccttc ttgatacaaa aggacctgaa atccgtacag aattgttcga aggtgaagct
     8221 aaagaatatt catacaaaac tggtgaaaaa attcgtgttg caactaaaca aggaatcaaa
     8281 tcaactcgtg aagtgattgc gttgaacgtt gctggtgctc ttgatatcta tgatgatgtt
     8341 gaagttggtc gtcaagtttt ggttgacgat ggtaaacttg gtcttcgtgt ggttgctaaa
     8401 gatgatgcaa ctcgtgaatt tgaagttgaa gttgaaaacg atggtatcat cgctaaacaa
     8461 aaaggtgtga acatccctaa cactaaaatt cctttcccag ctcttgctga acgcgataac
     8521 gacgatatcc gtttcggtct tgaacaaggt atcaacttca tcgcaatttc attcgtacgt
     8581 actgcaaaag atgtgaacga agttcgtgca atctgtgaag aaactggaaa cggacatgtt
     8641 caattattcg ctaaaatcga aaaccaacaa ggtatcgata acttagacga aatcatcgaa
     8701 gcagctgatg gtattatgat tgctcgtggt gatatgggta tcgaagtacc gttcgaaatg
     8761 gttccagttt atcaaaaaat gattatcaag aaagtcaatg ctgcaggtaa agttgttatc
     8821 actgcaacaa acatgcttga aacaatgact gaaaaaccac gtgcaactcg ttcagaagta
     8881 tcagacgtat tcaacgctgt tatcgacgga actgacgcta caatgctgtc aggcgagtct
     8941 gcaaacggta aatacccact cgagtcagta actacaatgg ctacaatcga caagaatgct
     9001 caagctcttc ttaatgaata cggacgtctt gattcagatt catttgagcg taactctaag
     9061 acagaagtaa tggcttctgc tgttaaagat gctactagct caatggatat caaattggtc
     9121 gtaactctta ctaagacagg tcatactgca cgtttgattt ctaaataccg tccaaatgct
     9181 gacatcttag cattgacatt tgacgaattg acagaacgtg gcttgatgtt gaactggggt
     9241 gttatcccaa tgttgacaga tgctccatct tcaactgacg atatgttcga aatcgctgaa
     9301 cgtaaagcgg tagaagcagg tctcgttgag tcaggcgatg atatcgttat cgttgctggt
     9361 gtgccagtag gagaagctgt tcgcacaaac acaatgcgta tccgcacagt acgttaagaa
     9421 aaatataaaa acctatcata tccagcttta gagcttgtgt gataggcttt ttgtatagag
     9481 ggtaagaaat aggcaaaact ttcataatgg attgatactc ttcgaaaatc aaattcaaac
     9541 cacg
//
LOCUS       DAVBHS010000079         9334 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVBHS010000079 DAVBHS010000000
VERSION     DAVBHS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.079"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(23..1159)
                     /gene="dnaJ"
                     /locus_tag="VQ651_001335"
     CDS             complement(23..1159)
                     /gene="dnaJ"
                     /locus_tag="VQ651_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET2754322.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(1364..1717)
                     /locus_tag="VQ651_001336"
     CDS             complement(1364..1717)
                     /locus_tag="VQ651_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754323.1"
                     /translation="MSVNKNNLSDIADYHEKIKFFEELFNLDYNPTEMLVTVERNGNV
                     IYKEFTEVAVGKSKYDHTSVFKDVMIYFSDHICQELHLYKTQDPHYNKFEFDLAKRSL
                     KITTRDNTTIFISNE"
     gene            complement(1730..3553)
                     /gene="dnaK"
                     /locus_tag="VQ651_001337"
     CDS             complement(1730..3553)
                     /gene="dnaK"
                     /locus_tag="VQ651_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET2754324.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(4033..4557)
                     /gene="grpE"
                     /locus_tag="VQ651_001338"
     CDS             complement(4033..4557)
                     /gene="grpE"
                     /locus_tag="VQ651_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET2754325.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(4584..5618)
                     /gene="hrcA"
                     /locus_tag="VQ651_001339"
     CDS             complement(4584..5618)
                     /gene="hrcA"
                     /locus_tag="VQ651_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET2754326.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTGLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLEAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(5782..6291)
                     /locus_tag="VQ651_001340"
     CDS             complement(5782..6291)
                     /locus_tag="VQ651_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HET2754327.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            6899..8836
                     /locus_tag="VQ651_001341"
                     /pseudo
     CDS             6899..8836
                     /locus_tag="VQ651_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            8834..9334
                     /locus_tag="VQ651_001342"
                     /pseudo
     CDS             8834..9334
                     /locus_tag="VQ651_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819352.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         2734 a         1904 c         1811 g         2885 t
ORIGIN      
        1 ttgaagagat tttcaaagag tattattctc catcaaaggc atctttaata tggtcaaaga
       61 agcctttttt ctttggattt actttcaagt caccagcagc cgcgaattct ttcaaggcta
      121 ctttttggcg gtcgttcaag cctgtcggtg ttacgacatt aacagtaacg tattggtcac
      181 caactgcacc gccacgaagg ctcggtgccc ccttactacg taggcggaat ttcttaccag
      241 tctgagttcc ctctggaata accaattcaa catcaccgtg aacagttgga atatctactg
      301 tatcaccaag agccgcttgg acaaagttga ggttgagatt gtagaagata gtcgttcctt
      361 cacgttcaaa cttgtcgcta gcttctacag aaactactac atacaagtcc ccataaggtc
      421 caccgttaaa gcctgcttcg ccttgaccag cgaggcgaat ttgttgacct gtttccacac
      481 cagcagggat tttcacatgt acgctatgag cttgtttctc atgacctgtt ccatgacagg
      541 ttgtacatgg atatttgatt tcttttcctc gaccgtgaca gacatcacag gttacttggc
      601 gacgcatcat accaagagga gtctgcgtat cgacgttaat gacaccagcg ccatgacagc
      661 gtccacaagt gactggactt gtccctggct tagcaccaga gccattacat gtacgacagc
      721 cagcttcacg atgatactta acttccttct cagttccgaa gatagcttct tcaaaggtca
      781 aattgacacg atactggaga tcatctcctt ggcgaggagc gtttggattg cgcgaagaac
      841 cgcctccgcc gaagaaactt gaaaaaatat cctcaaaacc accgaagcca cctgccccat
      901 tgaaaccacc gaaaccacca gctccaccaa aaccaccatt ggcgcctgca gcaccatact
      961 ggtcataggc agcacgtttt tggtcgtcac tcaaagtctc ataggcttct tgaacttcct
     1021 tgtacttgtc ctcagcacca ggctccttgt tgatatctgg gtgatatttt ttggaaagct
     1081 tacgataagc ctttttgatc tcgtctgccg aagcgttttt tgacaccccc agacgatcat
     1141 aaaattcagt attgttcata caagatacca agaccgtaaa attcgactga aaaataggaa
     1201 atctgacgct ggagcgacgc tcctagacag atttctcttt tttccgagaa tttaggtcgg
     1261 gttcaattcc tttcttttat attgatgaga taatactcca gccactcggc cctatccctt
     1321 tttaccgaaa aacagaggct gagttgcaac ctctgtggtt agattattca tttgaaataa
     1381 aaatggttgt attatctcta gtagtaatct tcaaagacct tttagctaaa tcaaactcaa
     1441 atttattata gtgtgggtct tgtgttttat ataggtgtaa ctcctgacat atatgatccg
     1501 aaaagtaaat cataacatct ttaaacacag aagtgtggtc atatttactc ttaccaacag
     1561 ctacttctgt aaattcttta taaatgacat ttccatttct ttccacagtc accagcattt
     1621 ctgttggatt ataatccaaa ttaaataatt cttcaaaaaa ctttatcttt tcatgataat
     1681 ctgcaatgtc agaaagatta tttttattaa cactcataac actagcccct tatttttcag
     1741 taaactctcc gtctacgacg tcatcgcctg cgtttcctgt tgcttgtgcg ccttctgctc
     1801 cttcttgagc ttgttgcgct gctgcagctt gttcgtagag tttaacagca agtccttgag
     1861 ctttttcgtt caatgcttca agttttgctt tcatgtcgtc caagttgttg tcttcttgag
     1921 ctttcttaag gtcatcaagg gcagcttggg cagcgtcacg ttctgcgtcg aagcctttac
     1981 cttcagtttc cttgattgtc ttttcagtcg caaagattgc ttggtctact tcattacgaa
     2041 ggtctacttc ttctttacgt ttcttatcgg cttcagcgtt tgcttctgca tctttcatca
     2101 tgcggtcgat ttcttcgtca gtcaaacctg agttcgattg gatgacaata gtttgttctt
     2161 tttgagttcc aaggtctttg gccttaacag acacgatacc gttcttgtcg atgtcaaatg
     2221 ttacttcgat ttgaggaatt ccacgaggtg cagctgggat atcagtcaat tggaagcgtc
     2281 caagagtctt gttatctgct gccattgggc gttcaccttg aagaacgtgg atatcaacgg
     2341 ctggttggtt gtctgctgct gttgagaaga cttgtgattt agatgttggg attgtagtgt
     2401 tgcgatcgat aagttttgta aatactccac ccattgtttc gataccaagt gacaatggcg
     2461 ttacatcaag aaggacaacg tccttgacat caccagtaat cacaccacct tggatagccg
     2521 cacccatagc aactacttca tcagggttta ctgatttgtt tggttcttta ccagtttcag
     2581 ctttaacagc ttcaacaacg gcagggatac gagttgaacc accaacaagg ataacttcgt
     2641 cgatttctga caagctcaaa cctgcatctg aaagggcttg acgaactgga acttttgtac
     2701 gttcaacaag gtcacgagtc aaatcgtcaa atttcgcacg agtcaaagtc atttccaagt
     2761 gaagaggtcc agcctcacct gcagtgataa atggcaagct gatttgtgtt gaagttacac
     2821 cagaaaggtc tttcttcgct ttttcagccg catctttcaa acgttgcatt gccatcttgt
     2881 cagtagacaa gtcgataccg ttttctttct tgaattctgc taccaagtgg tcaatgattt
     2941 tttggtcaaa gtcgtcacca ccaagtttgt tgtcccctgc agttgacaat acgtcgaaga
     3001 caccgtcacc caattcaagg atagagacgt cgaatgtacc accaccaagg tcaaatacca
     3061 agattttttc ttctttgtca gtcttgtcca aaccataagc aagagctgct gcagttggtt
     3121 cgttaacaat acgttctact tcaagaccag caattttacc agcgtctttt gttgcttgac
     3181 gttgagcgtc gttgaagtaa gccggaactg tgataacagc tttggttact ttctcaccaa
     3241 ggtagtcttc agcgtagcct ttcaagtatt gaaggatcat agctgagatt tcttgtggag
     3301 tgtattcttt tccatttgca gaaacttttt cagaagttcc catcttagat ttgatagaga
     3361 taactgtatc tgggtttgta actgcttgac gttttgcagc atcaccaacg atgatttctc
     3421 cgtttttgaa tgagactaca gatggagttg tgcggtttcc ttctgggttt gcgatgattt
     3481 tgctttcagt tccttcaaga actgcaactg ctgagtttgt tgtacctaag tcaataccga
     3541 taattttaga catgtgtttt tctccttgtt agttaatttt ctatttttct ttttgatact
     3601 cttcttaagt ggcgacgccg tcagagcttg acttcgtcaa tctctttgac taaactttga
     3661 gcctaaggtc tcaaaatttg cgcaaatagc accacggcga agagtatcgt ctttggttcg
     3721 cttcgctcac tattacaaag ccaaacatat tttatctttc ttcatagttt attgtcgttt
     3781 cggacaagtt ttctattatg taaattgcga cacgaggagt cgaaatcgat tttatttcga
     3841 cgacgagtta gtaaggaagc taggcaaacg tcatagcgat tgccgttttc tgacgagttg
     3901 ctttagctac cgtcagaatt gccgaatcga acacgcccta agctctgagt aaaaaagata
     3961 gattttcttg tgttcatcga acacttcgtc aatctcctat ttttactgcg agctttttac
     4021 gagctttgta tcttagttat acaccactac cattgctggg cgtaggatgc ggtcatggag
     4081 tttgtagcct ttttgaaaga cttgggcgat ggtatctact gggtgttcat cgtctgctgg
     4141 gagagtttgg atggccatat ggtagttatg gtcaaattcg ccatctgctg cgatttcttc
     4201 aattccttct tctttcaaag cgtgaatcaa gctttcttgc accatcccca agcccttctt
     4261 cacatcatct gtcaaacctt caactgcaag tgcacgctca aggttgtcaa gagatggtaa
     4321 gattgctttt gccaagtcct ggctacgata acgttgcaag ttttgacgtt cttcattggc
     4381 acggcgttgg atattttgca tttctgcatg agcgcgaaga tatttgtttt cgaactcatc
     4441 tgcacgttca tttgccaagt ccaactcaga cttttcagga gttgtttctt cagctgtttc
     4501 cacaacttcc tcttcttgaa cttcttctac ttcttcattt tttatatctt gggccatgtt
     4561 cgcctccttt aatgatttca atcttaatgt acttcgtaat gattactgct gaggtagcgg
     4621 taaaaatctg tcaacttcat ggtcaaaaca cggttgacca cattgacttg attgattagc
     4681 tgttggtaat ccagattaac tggaccgata atggctagaa ttccaactcc ccgataagga
     4741 atgaggaact tactactaat caccgctagg tcagctaaac aggactcttg accgtctgca
     4801 acacgaacat tttgcatctg atcctcacgc aacccctcac gaatctccaa ggccaccttt
     4861 tgcggttggt caaagaactg ataggcttct agattggcaa aattcaagag attgaccttg
     4921 cccgccatca caatgttttc gttgaacatt tccttaaaga tgtgttcaaa gagatcgatg
     4981 acattatccg ttgttgtaaa gtaacgctgg ataatctgcg gaatctccgt ccgaatcttg
     5041 tagtgaatat ctaaaacggt gtgaccgagg aaacgttcct gaatgatgct cttcagtttc
     5101 agcaaatcct cctgcaagaa gttccttgga atcagaaact gactagtaac cgttcgcgac
     5161 tcgtctaggg taaataccgc caaggctgta tgttgcccca aaacaacgat atcaaaggct
     5221 gtcaaacgtt gcctgctcgg ctcaacatcc agtgccacta ccgtacagcc actcaggcct
     5281 gttagcaagt tagcagcctc ttgcagaata tcctccaatt tgaagaattc ctgatcaaag
     5341 gctttgacaa tctcatatac ctcattttca gccagccggt gaaaatccag tgagtgtttc
     5401 acatagtact gaaaaccagc aacacttggc atccgaccgc ttgaagtatg agccttctca
     5461 agcaaccctt gtttttctag ttccgccatg tcattacgaa tggttgcact gctagagtta
     5521 atagactctt gcaaggcttt tgatccgaca ggttcgtgcg ttttggtaaa gatgtcaata
     5581 atcagattta aaatatcctg ctgacgctct gtaaccatct catcactcct ctctgtctga
     5641 tcaattagca ctcaatgcat ccaagtgcta acttatgatt ctattataca cccttaaaag
     5701 agaatgtcaa gacaaaaact caaaaaatta gcactctttt atcaagagtg ctaaaaatca
     5761 atctgaagac ctagaacctt gctattcatc tactcggtat ttccttttta gtctgaaata
     5821 accatgaatt agataagaaa tcagaaaagc atataaaaca aaaataagaa agaaagacat
     5881 agaaagatcc tctctaaaca aactcgtaca aacaacaaga ccacctaaga aagtagttgt
     5941 acaggaacga agtctagatc gcataacttc ccatttgagc cccttactca tatagacttg
     6001 atcctctgga agtaaatcag aagacgattt acgaagaatc gaacaagaac ctgctcctat
     6061 caattcccaa cctctatctc taaaatcttg cagttcatgc ttatattttt taagaaatct
     6121 agaatcatag atacggtaga tgacatcgtc tggttgacat tggtcaaaat agaacaaacc
     6181 aaaacgactc gttctatacc tccaaccttt caaatgcatc tcatgtaaat attcttcttc
     6241 cttgtccaaa tcaacaatgg tgaaaatccg aaattctact ctgctattca ttgtcttacc
     6301 ccaaaattag aaaacatgcc tggcgttatt tattagataa ttctttccac ttttgactca
     6361 atctccaaaa aatataagaa atctgaatcg caaaaactat caataaaacc caatctatta
     6421 tgaaaatcaa aaacactttc caactgaaag aactacctcc agtgacaaac tttgagcaaa
     6481 acggtagtag agctaaaaag agaagtaaaa taggaagcat ccgcattgtt aaaatccgtt
     6541 tggcataaaa aaatctttat ttaaactaaa atattatagc aaaatttacg ccagtttttg
     6601 aacggctgat gtagatattt tatactttca aaatgtttaa atgtgattat ttatttttga
     6661 aaaatagctc accagcccga ctgaaagtgc ttatagaatg ataataagtc gcctgccgaa
     6721 aacagcggaa aatagcggtg ttatgcggag ataatctgac gcgatgcgaa agtatattgc
     6781 atacttattt tcaacaattt agcagagtat ttttataagt gtgatataat agaagtataa
     6841 tttgttctga tagtttattt tatggagaag tagattttta gaatgcggag ggttcaatat
     6901 ggttgagttt ataaagtcta agaaagaaat gagtgaggag gatattaaag caaatttcat
     6961 cactcctgct attgtatcca aaggatggaa aaatggtgag catatcgctt acgaagaata
     7021 cttcactgat ggtcgaattg aggttagagg agataaggct cgtcgtaaag aaggaaaaaa
     7081 atcagactat tcactgtatt accaatttgg aactcgaatt gcaattgttg aggcaaagga
     7141 taataaacac agcgttcgag caggattaca acaagctatt gaatatggag agattttaga
     7201 tgttccattt gtttattctt cgaatggtga tggctttatt gaacacgacc gtatcacgag
     7261 agaagaacgt gagctggagt tagacgaatt ccctactcgt gaagaattat tttctcgtat
     7321 gacgaaggaa aaaggattga cgtacgaaat tacagaagct atctcaactc catactatac
     7381 agacgccttc tcaatgaaaa cgccacgcta ttatcagcaa atagctatca accgtactat
     7441 tgaaacagtt gccagaggac aaaaacgagt aatgtttgtg atggcaacag gaacagggaa
     7501 aacgttcatg gcttttcaaa ttattcatcg ccttcgaaaa gctggtttgg ctaaacgagt
     7561 tttattctta gcagatagaa acatcttagt agaccaaacg atggctgaag actttaagcc
     7621 attcgaaaag gtaatgacga aaattacacc aaaacttttg actgctcctg aaaaattaaa
     7681 ttctttcgaa atttatctag ggctttatca gcaactaact ggtgaagatg gaactgaaac
     7741 acattatcaa aaatttgaca aagacttctt tgatttaatc gtaattgatg aagcgcaccg
     7801 tggttcagct aaggaaaaca gtaactggcg taaggtaatt gattatttca gttctgcgac
     7861 acagattggg atgaccgcta ctcctaaaga aaccaagaat gcttccaata cggaatactt
     7921 tggtaagcca atctatactt atagtttaaa acagggaatc gaggatggtt ttttggctcc
     7981 atatcgtgtt atgagggtta atttagatgt ggatgtggat ggttatcgtc cagaaactgg
     8041 aaaagttgat gctaacggac aattaataga agataggtac tacggcagga aagattttga
     8101 taaaaccatt gtcattgatg atagaacgca aagagttgcc aagtttgttt ctgattatat
     8161 gaagcaaaac aatgcacgat ttgataaaac aattgttttt tgtgttgata ttgaccatgc
     8221 cgagcgaatg cgtgctgcac ttgtaaaaga gaatctagac ttagtccaag aagactatcg
     8281 ttatgtcatg caagtaactg gtgacaacgc tgaaggaaaa gctcaactgg ataactttat
     8341 ggatgtcaat tctaattttc ccgctattgt aacaacgtct aaattattaa cgacaggagt
     8401 taatgctaaa acatgtcgtt tgattgtttt agactctaat atccaatcca tgactgaatt
     8461 taaacaaatt attggtcgtg gcacacgtct ttatcctcaa aaggggaaag aattttttac
     8521 gattattgat tttcgaaatg ttaccaattt gtttgctgac cctgattttg atggtgagcc
     8581 agtgaaggtg ctggaaacag gtgcgaaaac agtcagtggt tctacgcctg gtttcgtagg
     8641 tgaggaaggt gacccagtag aaaaatatat cgttacagac aagcaggtta ccattcttaa
     8701 ttctactgtt caagtattgg atgaaaacgg gaaactgatt accgaaagcc tgaccgacta
     8761 cactcgaaag aatatcttag gtagctacgc cactttgaac gattttatca cagtttggca
     8821 tacggcagat aagaaacatt atgggaagtt tgctgatgga agcactcaag aaattgatgt
     8881 tccttatgat attcctgata cttgggagtg ggtgaggttt tctacattgg ttgaaattgt
     8941 cagaggtggc tctccacgac caatcaaaga ttatcttact tctgaagtag atggaataaa
     9001 ttggataaaa ataggtgata ctgaaaaggg tgaaaagtat ataaataatg ttaaagaaaa
     9061 aatcaaaaaa tcagggctta acaaaactag atttgtaaaa aaaggtacat ttttgttaac
     9121 taattctatg agttttggta gaccttatat tttgaatgtt gatggtgcaa tacacgatgg
     9181 atggttggct atttcgaact atgaaaactc attaaataaa gattacctat tctatattct
     9241 ttcatcaaat gtagtttatt ctcaatttct atctctaatt agtggagctg ttgtgaaaaa
     9301 cttgaatagt gataaagttg cttctattct tatc
//
LOCUS       DAVBHS010000080         9317 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVBHS010000080 DAVBHS010000000
VERSION     DAVBHS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.080"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            487..1530
                     /locus_tag="VQ651_001343"
     CDS             487..1530
                     /locus_tag="VQ651_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HET2754328.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGHNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFYTESEEQ"
     gene            1697..3784
                     /gene="topA"
                     /locus_tag="VQ651_001344"
     CDS             1697..3784
                     /gene="topA"
                     /locus_tag="VQ651_001344"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET2754329.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            4005..4364
                     /locus_tag="VQ651_001345"
     CDS             4005..4364
                     /locus_tag="VQ651_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HET2754330.1"
                     /translation="MRIIYLIIGFLSLTLAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQAYVADFRETKSIARERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4457..5089
                     /locus_tag="VQ651_001346"
     CDS             4457..5089
                     /locus_tag="VQ651_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HET2754331.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            5133..5789
                     /locus_tag="VQ651_001347"
     CDS             5133..5789
                     /locus_tag="VQ651_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET2754332.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVYMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5808..6902
                     /locus_tag="VQ651_001348"
     CDS             5808..6902
                     /locus_tag="VQ651_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET2754333.1"
                     /translation="MNFQLAKYSLLKRFLENIGFTTLEEYGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKILLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALVDKKQEITDADI
                     RALVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIF
                     NAIDKFFNQSVRLVSYTIDAVTDGIDTQDRVLVTVENRDTETIFNAAGLDFDVLKASA
                     IVYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6914..7951
                     /gene="leuB"
                     /locus_tag="VQ651_001349"
                     /pseudo
     CDS             6914..7951
                     /gene="leuB"
                     /locus_tag="VQ651_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            7948..8217
                     /locus_tag="VQ651_001350"
     CDS             7948..8217
                     /locus_tag="VQ651_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HET2754334.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKSKARRRVWRIPEKILLIL
                     AFTFGGFGAWLAGIIFHHKTRKWYFKIVWFLGMVTTLVALYFIWR"
     gene            8221..8580
                     /gene="leuD"
                     /locus_tag="VQ651_001351"
     CDS             8221..8580
                     /gene="leuD"
                     /locus_tag="VQ651_001351"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET2754335.1"
                     /translation="MAGSSREYAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8742..9310
                     /locus_tag="VQ651_001352"
                     /pseudo
     CDS             8742..9310
                     /locus_tag="VQ651_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         2884 a         1624 c         2079 g         2730 t
ORIGIN      
        1 aaggtgcact gttatagatt caatacacta taatttagtg taattgagaa aggagaaatg
       61 attgtgattg atgttggcta ggttatgttc aatgattcct accgtctcaa atcttgtcag
      121 taaggaaaaa taaattcttc aaaagtagag attacaaggc ttgtttaaga aagaattcaa
      181 agaccttgac aaataaaaat aaaatggtta ttataaaaaa tggtctgaaa tagatgatga
      241 tacttttcga aaatcttttt acgtcagctc agctttgcct tgctgtgttt tgagcaagct
      301 acggttagct tccgagtttg attttcattt actagaaatg aaactgatga gagatatcag
      361 tagacatttg agtcaggata ttatggaaaa tgataaaaag agctcgtgag attggcatat
      421 cagactacta aagtattgag tttgttagga ttttagcgac tagttagctg ggaaaggaag
      481 atatttgtga caaataataa actgtattcg ttgatagaat ttagaaataa aatatatgaa
      541 gaattagaac tttccagaag tgatttagcg attttactat gtgccatgct tatcgcctct
      601 atcggattaa atatggattc gactcccgtg attattggag ccatgttaat ctctcctttg
      661 atgacaccta ttctgggagt ggggctctct ctagctatat ttgattttaa attgttaaga
      721 aaatctttta aaatattagc tattcaaatt cttgccagtt taatagcttc aacactttat
      781 ttttatcttt ctcccatttc gtatgctagt tcggaaattg ttgctagaac ctctccgact
      841 atttgggatg ttctcattgc ttttgtagga gggatagcag gtattattgg tgctaggaaa
      901 aaagagacca ataatattgt tcctggtgtt gctattgcaa ccgccttgat gcctcctctt
      961 tgtacagtag gttatgctat tgcttctgct aatctaaaat ttatcatagg ctcctcttac
     1021 ctattcctca tcaattgtag ctttattgtc attgcgactt atataggtgt taggttgatg
     1081 atggttaaga aacattattt taaagataat gaagaagact ctaaaatgcg taggattttg
     1141 cttctagttg ctgttttgct gatgattccg agtttcatct ctgcaacgac tttagtgaga
     1201 gaaacgttga aaaaagagtc ccttaagaaa tttatatcag agcagtttca ggggcataat
     1261 attttgaaaa aaacctattc taaaaagact cataccctaa agctaaccat ttcaggaaat
     1321 tatttgacag aagaagaact cgatatgatt tcaagtaaga gaggtgacta tggtttaagt
     1381 gatgtttctg ttcaagtttc acaattgtct gattcagaac aacttagtaa ggaagaactg
     1441 gtggagtatt tcttccagta tatcaaggat aaggaagcaa aagaaaagga aaaagctaat
     1501 aagttttata cagagtctga ggagcaataa tttcttgaga atagctggtt tctctcgtga
     1561 gtcttctatg tatatcaaag gaagactgag gttttaagta taaaactttt cttctattat
     1621 agtagctaag tcttgacagg gttcaaaaaa tggtttacac tttataaagt ttattacttt
     1681 gaaaaggtgt gatactgtgg ctacggcaac aaaaaagaaa aaatcaacag ttaaaaaaaa
     1741 tctagtcatc gtggagtcgc ctgctaaggc caagacgatt gaaaaatatc taggcagaaa
     1801 ctacaaggtt ttagccagtg tcgggcatat ccgtgatttg aagaaatcca gtatgtccgt
     1861 cgatattgaa aataattatg aaccgcaata tatcaatatc cgaggaaaag gccctcttat
     1921 caatgacttg aaaaaagaag ctaaaaaagc taataaagtt tttctcgcga gtgacccgga
     1981 ccgtgaagga gaagcgattt cttggcattt ggcccatatt ctcaacttgg atgaaaatga
     2041 tgccaaccgt gtggtcttca atgaaatcac caaggatgca gtcaaaaatg cttttaaaga
     2101 acctcgtaag atcgatatgg acttggtcga tgcccaacaa gctcgtcgga tcttggatcg
     2161 cttggtaggg tattcgattt cgcctatttt gtggaagaag gtcaagaagg gcttgtcagc
     2221 aggtcgcgtt cagtccattg cccttaaact catcattgac cgtgaaaatg aaatcaatgc
     2281 cttccagcca gaagaatact ggacagttga tgctgtcttt aaaaagggaa ccaaacaatt
     2341 tcatgcttcc ttctatggag tagatggtaa aaagatgaaa ctgaccagca atgacgaagt
     2401 caaggaagtc ttgtctcgtc tgactagtaa agacttttca gtagatcagg tggataagaa
     2461 agagcgcaag cgcaatgctc ctttacccta taccacttca tctatgcaga tggatgctgc
     2521 caataaaatc aatttccgta ctcgaaaaac catgatggtt gcccaacagc tctatgaagg
     2581 aattaatatt ggttctggtg ttcaaggttt gattacctat atgcgtaccg attcgactcg
     2641 tatcagtcct gtagcgcaaa atgaggcggc aagcttcatt acggatcgtt ttggtagcaa
     2701 gtattctaag cacggtagca aggtcaaaaa cgcatcaggt gctcaggatg cccatgaggc
     2761 tattcgtccg tcaagtgtct ttaatacacc agaaagcatc gctaagtatc tggacaagga
     2821 tcagctcaag ctatataccc ttatctggaa tcgttttgtg gctagccaga tgacagcggc
     2881 cgtttttgat accatggctg ttaaattgtc tcaaaatgga gttcaatttg ctgccaatgg
     2941 tagtcaggtt aagtttgatg gttatcttgc catttataat gattctgaca agaataagat
     3001 gttaccggac atggttgttg gagatgtggt caaacaggtc aatagcaaac cagagcaaca
     3061 tttcacccaa ccgcctgccc gttattctga agcaacactg attaaaacct tagaggaaaa
     3121 tggggttgga cgtccatcaa cctatgcgcc aaccattgaa accattcaga aacgttatta
     3181 tgttcgcctg gcagccaaac gttttgaacc gacagagttg ggagaaattg tcaataagct
     3241 catcgttgaa tatttcccag atatcgtaaa cgtgaccttc acagctgaaa tggaaggtaa
     3301 actggatgat gtcgaagttg gaaaagagca gtggcgacgg gtcattgatg ctttttacaa
     3361 accattctct aaagaagttg ccaaggctga agaagaaatg gaaaaaatcc agattaagga
     3421 tgaaccagct ggatttgact gtgaagtgtg tggcagtcca atggtcatta aacttggtcg
     3481 ttttggtaaa ttctacgctt gtagcaattt cccagattgc cgtcataccc aagcaatcgt
     3541 gaaagagatt ggtgttgagt gtccaagctg tcatcaggga caaattattg agcgaaaaac
     3601 caagcgtaat cgcctattct atggttgcaa tcgctatcca gaatgtgaat ttacctcttg
     3661 ggacaagcct gttggtcgtg actgtccaaa atgtggcaac ttcctcatgg agaaaaaagt
     3721 ccgtggtggt ggcaagcagg ttgtttgtag caaaggcgac tacgaggaag aaaagattaa
     3781 ataagaggag gggcccgaaa gtaaatttca ggctcctttt ggttagaact tgacaaaata
     3841 catcatttta tgcgaaacta gaagaagatt attttatact caatgagaat caaagagcaa
     3901 attagaaagc tagtcgcagg ttgctcaaag cactactttg aggttgaaga tggaagctga
     3961 cctggtttga agagattttc gaagagtatt aactaggaga agttatgcgt attatctatc
     4021 taattattgg ttttttatcg ctgaccttgg ctattgttgg ggttgtttta cccttgttgc
     4081 ctacaacacc tttccttttg ttgtctattg cttgtttctc cagaagttcc aagcgattcg
     4141 aagattggct ttatcatacc aagctctatc aagcatatgt agctgatttt cgtgagacca
     4201 agtctattgc gcgtgaacga aagaaaaaaa tcatcgtctc tatctacgtc ttgatgggaa
     4261 tttctattta ttttgcacct cttttaccag tcaaaatcgg tctgggtgct ttgaccatct
     4321 ttattactta ttatctcttc aaggtcattc cagacaaaga atagttaaaa cagtagttat
     4381 ttgccttgat aaaattgaaa gcatattcat aacaatatga tataataaaa ttgaagtaat
     4441 attcaaggag aatcaaatga tttacgaatt ttgtgctgaa aatgtgactt tacttgaaaa
     4501 agcgatgcag gctggagctc gtcggattga actctgtgat aatctagcag ttggtgggac
     4561 aacacccagc tatggagtga ctaaggcagc ggttgaactg gcagctaact acgatacaac
     4621 catcatgacc atgattcggc cacgtggtgg tgactttgtc tataatgacc tagaaattgc
     4681 tatcatgcta gaagacattc gtttgactgc tcaggctgga agtcaagggg ttgtatttgg
     4741 agctttaact gctgataaaa agttggataa gcctaatctg gaaaagttaa ttgctgcatc
     4801 aaaaggaatg gaaattgtct ttcacatggc ctttgatgaa ctaagtgatg aagatcaacc
     4861 ggaagctatt gactggctca gtcaagccgg tgtcactcgt atcctaactc gtgctggtgt
     4921 gtctggcgac tccttagaaa aacgttttgt tcactatcac agaattttgg agtacgctaa
     4981 aggtaaaatt gaaattctac caggtggggg gattgacctt gaaaaccgtc aaacctttat
     5041 cgaccaggtg ggggtaacac aattgcatgg tactaaggtt gttttttaaa aaatagaaag
     5101 gaactgctag ctttgggtag cagttttcac ttatgtttga aatttttaaa tcctatcaat
     5161 ttaatcaaga aaaggctcat gattatggtt ttatagaaaa tagcgaagtc tggacatata
     5221 gttgccagat tttgcaaggt gactttgtca tgactgtgtc catcactgct gataatgtga
     5281 actttcaagt ctttgaccaa gagactggtg acctctatcc tcacgtttat atggaaagca
     5341 tgaggggaag ttttgtcgga aatgtccgtg aggcttgtct ggagattctt taccagattc
     5401 ggaaggcttg ttttgatgtg caagatttta tctgtcatca gactaagcgt atcatgactc
     5461 aagttcagga aaagtatgga aaccagttgg agtatctgtg ggaaaaatcg cctgatacag
     5521 ctgtattgcg ccatgaaggc aatcaaaagt ggtatgccgt cttgatgaaa atctcttgga
     5581 ataagctgga aaagggcaga gaaggacaag tggaagcagt caacctcaag catgaccaag
     5641 tagctaattt gctttcacaa aaggggattt atccagcctt ccatatgagc aagcgctact
     5701 ggattagtgt gtcccttgat gatactttat cagatgaaga agtactggaa ttgatagaaa
     5761 aaagttggaa cttaacctct aaaaaatgaa atattttaat aattttcatg aactttcaat
     5821 tagctaaata ttctttactg aagagatttt tagaaaatat aggatttacc acactagagg
     5881 aatatggtgc catcttcaaa tacctgattg agaatgtcaa gacggatcgt cagatcatct
     5941 attcgcctca ctgtcatgat gacctcggaa tggcagtggc aaatagcctt gctgctgtca
     6001 agaatggtgc aggacgtgtt gaagggacta tcaatggtat tagggagcga gctgaaaatg
     6061 ctgctttgga agaaattgca gtggctctca atattcgcca agattactac caagtagaaa
     6121 ccagtattgt cctaaatgag accatcaata cgtcagaaat ggtttctcgc ttctctggta
     6181 ttccagttcc taaaaacaaa gccgtcgttg gtggcaatac cttctcccac gaatctggta
     6241 ttcaccaaga tggagtcctt aaaaatcctc tcacttatga gatcatcaca cctgaattgg
     6301 ttggtgttaa gattctgctt ggaaaattat ctggtcgcca tgcttttgtt gagaaactga
     6361 gagaattggc cctagatttt acagaagagg atatcaaacc actctttgct aagttcaagg
     6421 cactggtcga taagaagcaa gaaatcacag atgcagatat tcgagctttg gtagctggaa
     6481 ccatggttga aaatccagaa ggcttccact ttgatgattt acaacttcaa actcatgcag
     6541 ataatgacat tgaagcgctc gttagcctag ccaatatgga tggtgagaaa gtcgaattta
     6601 atgcgacagg gcaaggttcc gttgaagcaa tctttaatgc tatcgataag ttctttaacc
     6661 aatctgttcg tttggtgtcc tacactatcg atgcggtaac agatggaatc gatacccagg
     6721 atcgggtttt ggtcactgtt gaaaacagag atacagaaac catctttaat gcagcagggc
     6781 ttgattttga tgtgttgaag gcttctgcta ttgtctatat aaacgctaat acctttgttc
     6841 aaaaagagaa tgcaggtgag atgggacgca gtgtttctta ccacgatatg cctagtgtgt
     6901 aaaggagaag gctatggcaa agaaaatagt agctctagca ggagacggaa ttggcccaga
     6961 aatcatggag gttggtttag aagttctgga ggctctagct gaaaaaacag gttttgacta
     7021 tgagattgac agacgaccgt tcggaggtgc agatattgat gcagcatgac ctcccttacc
     7081 tgatgaaacc cttaaggcaa gtagggaagc agatgctatc ctactagtag ctatcggtag
     7141 tcctcagtat gatggagcag tggttcgccc tgaacaaggc ctgatggctc tccgtaagga
     7201 actcaatctt tacgctaata ttcgtcctgt aaaaatcttt gacagtctca agcatttgtc
     7261 actactcaaa ctggaacgaa ttgctggtgt agactttgtc gtggtgcgtg aattgacagg
     7321 cgggatttac tttggagatc atattcttga agagcgcaat gcgcgtgata tcaacgacta
     7381 tagctatgag gaagtggagc ggattattcg caaagccttt gaaattgcaa gaaatcgcag
     7441 aaaaatcgtt actagtatcg ataagcaaaa tgttctagcg acctcaaaac tctggcggaa
     7501 agtagctgag gaagtcgcac aggatttccc agatgtaacc ttggaacatc agctggtaga
     7561 ctcagctgct atgcttatga ttaccaatcc tgctaagttt gatgttattg taacggagaa
     7621 tctttttgga gatattttat ctgatgaatc aagcgtctta tctggtacac ttggggttat
     7681 gccatcagcc agtcattctg aaaatggacc aagtctctat gaacctattc acagttcagc
     7741 acctgatatt gcaggtcaag gaattgccaa tcctatttcc atgattttat cagtttccat
     7801 gatgttgaga gatagtttcg gacgttatga ggatgcagag cgtatcaaac gtgctgttga
     7861 gacaagtctg gcggcaggaa ttttaacgag agatatagga ggtcaggctt caacaaagga
     7921 aatgacggaa gctattattg caaggttatg aagttagacg aaaaaattac tctagtcctt
     7981 ttgatttgga atgtcatcat tttcttgatt tatggtattg acaaatctaa ggcaaggaga
     8041 agagtttggc gcatccctga gaaaatctta cttattttag cctttacttt tggtggtttt
     8101 ggtgcctggc tagcaggaat catctttcac cacaagactc gaaaatggta ctttaaaata
     8161 gtttggtttc ttgggatggt gaccacacta gtagccttat attttatttg gaggtaatgg
     8221 atggcagggt cttcgaggga atacgctgct tgggctctag cggactatgg ttttaaggtc
     8281 gtgattgcag gatctttcgg tgacattcat tacaataatg aactcaataa tggcatgttg
     8341 ccaatcgttc agcctagaga ggttagagag aaactagccc agctaaaacc aaccgaccag
     8401 gtaactgtgg acttggaaca acaaaaaatc atctcaccag ttgaagaatt caccttcgag
     8461 atagatagcg agtggaaaca taaactccta aatagtttgg atgatatcgg tattaccttg
     8521 cagtatgaag agttgattgc tgcttatgaa aaacaacgac cagcctactg gcaggattag
     8581 aaaaaataga aaaggagata tagtaaactg aaataagatg taaacaaatg aattggagct
     8641 taacatccat ttccagcaat tttttagaaa ctacagtgga ctattctgga ttcaacacat
     8701 tataaaatta tgacaaaaca cattcacaag aaggctacga cattttaaaa ggtgagggcg
     8761 gatgtatcgt ttgccctact aaagttggtt acattatcat gaccagtgac aaggcaggac
     8821 ttgagcgtaa gttcgcagcc aaagaacgta agcgtaacaa accaggtgtt gttctctgcg
     8881 gtagcatgga tgaactttgc gctttagcgc aactcaaccc agaaattgaa gcattctact
     8941 aaaaacattg ggatgaagat attcttcttg gttgtatcct tccttggaaa ccagaagcct
     9001 ttgaaaaact caaagcatac ggggatggcc gtgaagaact tatgactgat gtacgtggta
     9061 ctagctgttt tgttatcaag tttggaaaag caggtgaaca attggctgcc aagctttggg
     9121 aagaaggtaa aatggtctac gcctcatctg cttcaatgac aaaacgattg aaactcgcta
     9181 tgagcaaggt gtaatggtgt ctatggtcga taaggacggc aaactcatcc cagaacaagg
     9241 aggagcacgt tcaacttcac cagctccagt tgtgatccgt aaagggcttg acattgatat
     9301 agtaaaatga agtaaga
//
LOCUS       DAVBHS010000081         9289 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVBHS010000081 DAVBHS010000000
VERSION     DAVBHS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9289
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.081"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..389)
                     /locus_tag="VQ651_001353"
     CDS             complement(<1..389)
                     /locus_tag="VQ651_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET2754336.1"
                     /translation="MKTFFAKKRNIFLARLFLGQLPLLVSTYLFLSRQFLNFSVVFQF
                     LLVVINLASILVTVYLTREMRIREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITA
                     GESYQQIIAYIGAVLCLIIMLLLMWGL"
     gene            complement(386..550)
                     /locus_tag="VQ651_001354"
     CDS             complement(386..550)
                     /locus_tag="VQ651_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754337.1"
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIAYVIAVMIYF
                     AINKKKGEKK"
     gene            complement(849..968)
                     /locus_tag="VQ651_001355"
     CDS             complement(849..968)
                     /locus_tag="VQ651_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HET2754338.1"
                     /translation="MDFKSFIIGLVVGIFGPYMYNLIRKIFSKFPKKDTDSTP"
     gene            complement(971..1201)
                     /gene="blpU"
                     /locus_tag="VQ651_001356"
     CDS             complement(971..1201)
                     /gene="blpU"
                     /locus_tag="VQ651_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HET2754339.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAARGLQ
                     LGIKTGTWQGAATGAAGGAILGGVAYAATCWW"
     gene            complement(1270..1539)
                     /gene="blpJ"
                     /locus_tag="VQ651_001357"
     CDS             complement(1270..1539)
                     /gene="blpJ"
                     /locus_tag="VQ651_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HET2754340.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            complement(2006..2203)
                     /gene="blpI"
                     /locus_tag="VQ651_001358"
     CDS             complement(2006..2203)
                     /gene="blpI"
                     /locus_tag="VQ651_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HET2754341.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            2485..3072
                     /locus_tag="VQ651_001359"
     CDS             2485..3072
                     /locus_tag="VQ651_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HET2754342.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            3065..4654
                     /locus_tag="VQ651_001360"
                     /pseudo
     CDS             3065..4654
                     /locus_tag="VQ651_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
     gene            4665..6026
                     /locus_tag="VQ651_001361"
     CDS             4665..6026
                     /locus_tag="VQ651_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HET2754343.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMDESQKTAL
                     ETQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLANSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LIASQGQTQGTAEEPFLSQINQSIASLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLLLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     SIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            6083..6238
                     /gene="blpC"
                     /locus_tag="VQ651_001362"
     CDS             6083..6238
                     /gene="blpC"
                     /locus_tag="VQ651_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HET2754344.1"
                     /translation="MDKKQNLTSFQELTTTELNQIIGGGWWEDFLYRFNIIEQKNTKG
                     FHQPIQL"
     gene            complement(6282..7622)
                     /locus_tag="VQ651_001363"
     CDS             complement(6282..7622)
                     /locus_tag="VQ651_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2754345.1"
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGISLTFERIFKAFLFKILL
                     AVVFGMLGYMVGNTYLSYFMEPLYGIGLSFLLLRELPKKLLLFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDDYSFIWLCIIIFNFFISLAFLKWLDYDFTSLRKGILDKDFQK
                     SLTQINWIMGAYYLVIQNLSYFEYEQGIQSTTVRHLILVFYLLFFMGIIKKLDTYLKD
                     KLHERLNQEQDLRYREMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLKDSSEKLQDNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTVVSILCDNAIEASVEACQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQDHVFRQVLTVIHIE"
     gene            complement(7636..8373)
                     /locus_tag="VQ651_001364"
     CDS             complement(7636..8373)
                     /locus_tag="VQ651_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2754346.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKEHHITPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSTRPHRVILYTKTDRLEFTASLEEVLKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(8378..8716)
                     /locus_tag="VQ651_001365"
     CDS             complement(8378..8716)
                     /locus_tag="VQ651_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2754347.1"
                     /translation="MKHMIIQTQKTVYKVNVDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQYGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            8993..>9289
                     /locus_tag="VQ651_001366"
     CDS             8993..>9289
                     /locus_tag="VQ651_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET2754348.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTK"
BASE COUNT         3097 a         1918 c         1456 g         2818 t
ORIGIN      
        1 ttcaagcccc acatgagcag aagcatgatg attaagcaga gaacagcgcc aatataagcg
       61 attatttgtt ggtaggattc tcctgctgtg atacctctat acaaacaaat aatagacata
      121 aaacctgtca agccgatgaa cataagttga ttggttctag gactaaccaa atcatcatct
      181 tcaaactctc ttatcctcat ttccctagtg agataaacag taaccaaaat agaagccaag
      241 ttaataacta ctaaaagaaa ttggaaaact acggaaaaat ttaaaaactg acgagataga
      301 aatagataag tagaaacaag caagggcaac tgacctaaga acaatctcgc aaggaagatg
      361 ttccgttttt tagcaaaaaa agttttcatt tcttttctcc tttcttttta ttgatagcaa
      421 aatagatcat aactgcaatc acataggcta tggtataaaa tagctgatac caagcactct
      481 ccctaagcgg atatagaaag atggacatga ttagatacag aacgaaaata atcagtattt
      541 ttttcttcat aagatttcct cctaaatgtg cgatttatct tagttgagca agaacattta
      601 cactgctagt atagcactta ttttgacctt ggatcactca aatcataaat ggtcatcgaa
      661 acatcttgaa ttgttaaaaa atttaaaaag taagcattaa aaacatactt tcctctttat
      721 attgtattga taccaacttg tttgtagact tttcatcctg ctatcacata tcattttgac
      781 aggcgaaaca aaattaaaga aactcccctg taaattaagc tagcaaatac aggggagaaa
      841 tttatttttt agggggtact atctgtatcc tttttgggaa attttgaaaa tatttttcta
      901 attaaattat acatataagg accaaatata ccaactacta aaccaataat aaaactttta
      961 aaatccataa ttaccaccaa catgttgctg cataggctac acctccaagt atagctccac
     1021 ccgcagcacc agttgctgca ccttgccatg ttcctgtttt aatgcctagt tgaagacctc
     1081 ttgctgctgc tcctccaaca cctgctttgg caaaatctcc ccaattgcat ccgccacctt
     1141 caacgcaagc aagcatttca gtatccataa cagaaaattg tgacatcatt tttgtatcca
     1201 tgacaaatac tcctttttta aaaaactaaa ataaatcaga atagaatcct cataatttta
     1261 ctataagtct taccaactta gtcccaattt atcaccaacc atacctccta agcatgttaa
     1321 tccaccccca attgcaccaa tgtgtgctcc aacaaatgca ccagcaagtc cagctactcc
     1381 taaagtggcc aaacctgctc cagttccacc agttataatt cccgtagtga ctcctgtaat
     1441 cagtgcattt tgacaatcag tggagctata ccccccttca actttcgcaa gcatttcagt
     1501 atccataacc tctaactgtg acaacatttt tgtattcatg atgaatacct cctttttatt
     1561 ttcaatttgt taccaaagtc ttaaattcaa taaacaaata gattttttat agtatctttt
     1621 tgattttctt aaaaaagtat atacgtctac tatcttctta aaggtagcag tacctatttt
     1681 ttagtctaag atttcaataa tcttgagtat ctaaaatatc ttaatttcgt tattctcctt
     1741 gcaataaaaa gttttactat actatttatt aacttgcaga aagcaaaaaa tattagtaaa
     1801 taatagttta tagttaagtt ttttattcct accaatccat caactaagta aagcatcaac
     1861 gattacataa acgattgata atataattaa aattttgcta actatcttat tctcatcatt
     1921 cttagataac tttgatattt tgtaagtaag taaataagac agtaaattaa tagcgataat
     1981 aatactatat ttaagaatca taatcttaca aagaggacat aattcctgaa cctacacaaa
     2041 taagtgttgc tgctccccca gttatcggac cagtcgcagc agctaatagt actgctccaa
     2101 tacaaccacc gattgcagat cctaaattgc ctcttcctcc actaactatt tcgagttctt
     2161 cattatccat aacagaaaat tgttccatca tttttgtatt catgacaaat actccttttt
     2221 tcttttttta tttttgtctt gttgtaactt tgataagttt agtatatcat cgttttttaa
     2281 aatttttcat ccagatcttg aattgtcatc gaaacgtctt gaattagctt ttttatttca
     2341 agccacctct aaatgtttaa aaaaaataat ttctaatcac ttttttacca ttcaggaagt
     2401 tttaatgact attcaagatt tcataaaata tgaacttagt tttatgacat aatagaccta
     2461 tccactatat gaaaagaatt gccaatgact tcttataaac gtacatttgt tcctcaaata
     2521 gatgcgagag actgtggtgt cgctgcctta gcctcgattg ctaaattcta tggttcagat
     2581 ttttctctag ctcacttgag agaacttgca aagaccaata aagaagggac gactgctctt
     2641 ggcattgtaa aagccgctga tgaaatgggc tttgaaacaa gacctgttca agcagataaa
     2701 acgctctttg acatgagtga tgtcccctat ccatttatcg ttcacgttaa caaagaagga
     2761 aaactccaac attactatgt tgtctatcaa acaaagaaag actatctgat tattggtgat
     2821 cctgaccctt ctgtaaaaat cactaaaatg tcaaaagaac gctttttcta tgaatggact
     2881 ggagtagcta tttttctagc taccaaaccc agctatcaac cccataaaga taaaaagaat
     2941 ggtctactaa gcaagcttcc ttcctctgat tttcaaacaa aaatctctca ttgcttacat
     3001 tgttctctca agcttattgg tcactattat caatataggt ggttcttact atctccaagg
     3061 aatcttggat gaatacattc caaatcagat gaaatcaact ttaggaatca tctcagttgg
     3121 tctggttatc acctaggtta tcacctatat cctccaacaa gtcatgagct tctccagaga
     3181 ttatctccta accgttctga gccagagatt aagcattgat gtgattttat cctatattcg
     3241 ccatattttt gaacttccca tgtctttctt tgcgacacgt cgtacaggag aaatcatttc
     3301 acggttcaca gatgctaact ctattataga tgccttggct tctaccattc tttctctttt
     3361 tctggatgtt tctattctga ttcttgtagg gggagtcttg ctggcacaaa accctaatct
     3421 cttcctcctt tctcttcttt ccattcctat atacatgttc atcatctttt cttttatgaa
     3481 accttttgaa aaaatgaacc atgatgtcat gcaaagtaat tctatggtta gctctgccat
     3541 tatcgaagat atcaacggga ttgaaactat aaagtcgctc acgagtgaag aaaatcgcta
     3601 tcaaaatata gacagcgaat ttgtagatta tttggaaaaa tcctttaagc tcagtaaata
     3661 ttctatttta caaacgagtt taaagcaggg aacaaaatta gttctgaata tccttatcct
     3721 atggtttggc gctcaattag tcatgtcgag taaaatttct atcggtcagc tgattacctt
     3781 taacacactt ttttcttact ttacaactcc tatggaaaat attatcaacc tccaaaccaa
     3841 actcccatct gcgaaggtcg ctaataaccg tttgaacgaa gtctatctag tcgaatctga
     3901 atttcaagtt caagaaaacc ctgtccattc acattttttg atgggcgata ttgaatttga
     3961 tgacctttct tataagtatg gttttggacg agatacctta acagatatta atctcacgat
     4021 taaacaagga gataaggtta gcctagttgg agttagtggt tctggtaaaa caactttagc
     4081 caaaatgatt gtcaatttct ttgaacccta caaagggcat atttccatca atcatcagga
     4141 tattaaaaac attgataaaa aagtcttgcg ccgtcatatt aattacctac cccaacaagc
     4201 ctatatcttt aatggctcta tcttggaaaa tttaaccttg ggcggtaatc atatgattag
     4261 ccaagaagat attctaagag cttgtgaatt agctgaaatc cgtcaagaca ttgaaagaat
     4321 gcctatgggc tatcaaactc agctctctga tggagctggt ctatcaggag gacagaagca
     4381 acgaatagcc ctcgctcgtg ctcttttaac taaatctcct gttttaatac tagacgaagc
     4441 tactagcggt cttgatgtct tgactgagaa aaaagttata gataatctta tgtccctaac
     4501 tgataaaacc attctctttg tagcccatcg tctcagtata gccgaacgaa ctaaccgtgt
     4561 cattgttctt gaccagggga aaatcattga agttggtagt caccaagagt taatgcaggc
     4621 gcaaggcttc taccatcatc tgttcaataa ataaggagaa tgtcatgaat cctaatcttt
     4681 ttagaagcgt cgagttttat cagagacgtt accataacta tgcgacagtg ttaattatac
     4741 ctctttcatt actgtttact ttcatcttga ttttctccct tgttgccaca aaagaaatta
     4801 ctgttacttc ccaaggagaa atcgccccta caagtgtcat tgcatccatt cagtcaacca
     4861 gtgataatcc tatccttgct aatcatttag tggcaaatca agtagttgaa aaaggtgact
     4921 tactcatcaa atactctgaa acaatggatg agagtcagaa aactgcctta gaaactcaat
     4981 tacaaagact tgagaagcaa aaagaaggac ttggaatttt gaaacaaagt ttagaaaaag
     5041 cgactgatct tttttctggc gaggatgagt ttggctacca taataccttt atgaatttta
     5101 ctaaacaatc ccatgatatt gaactgggta tcacaaagac taacaccgaa gtttcaaatc
     5161 aagctaatct tgccaatagc agttcatcag ccatcgaaca agaaattaca aaagttcaac
     5221 aacaaattgg agaatatcaa gagttgagag atgctatcat aaataacaga gcacgcttac
     5281 caactggcaa tccgcaccag tcaattttaa atcgttatct tatagcctct caaggacaaa
     5341 cacaaggaac ggcagaggag ccatttttat ctcaaattaa tcaaagtatt gcaagtcttg
     5401 aatcatctat cgcaagcctc aaaattcagc aagctggtat cggaagtgta gcaacttatg
     5461 ataatagttt agcaaccaaa attgaagtac tccgcactca gtttttacaa acagcctcac
     5521 agcaacaact aaccgtggag aatcaattaa cagaattaaa agtacaacta gatcaagcga
     5581 cacagcgctt agaaaataat accttaaccg ctccaagtaa aggtatcgtt catctgaaca
     5641 gcgaatttga aggtaaaaat agaattccaa ctggtacaga aattgctcaa atattccctg
     5701 tcatcacaga tacaagagaa gtactaatca cttactacgt atcatctgac tatctcctct
     5761 tactagataa aggacaaact gtaagattaa aactggagaa gattggaaat cacggcacca
     5821 ccatcatcgg ccaacttcag agtattgatc aaactcctac cagaacagag caaggcaatc
     5881 tctttaaatt aaccgctctt gcaaaactat ctaacgagga tagtaaactc atccaatatg
     5941 gcttacaagg tcgcgtcact agtgtaacta caaagaaaac atattttgat tatttcaaag
     6001 ataaaatttt aacacattct gattaatttt cagataacac tctataacta tttattatct
     6061 tatcaaaaag gagaatcata acatggataa gaaacaaaac ctaacttcat ttcaagaact
     6121 aacaactacc gaactcaacc aaattatagg tggaggatgg tgggaagatt tcttatatag
     6181 atttaatata attgaacaaa aaaatacaaa aggatttcat cagccaatac aactataaaa
     6241 aaacaagacc aagagaacgc actctcggtc ttattttttc ttcattctat atgtattaca
     6301 gtaagtacct gacgaaaaac gtgatcttgg caggtggtat tgagactggt attgggatga
     6361 ctttccacaa ttttcataac ggtatagaga ccaactcctc tctcctcccc tttagaactt
     6421 gctccaaagg agaagatttc agaaatatcg attccctctt ctttgatgga attttcaatg
     6481 ataaaggtct cctgtgctcc attttttaaa aaggcaattg aaacatgagg ttgacaggct
     6541 tctacactag cttcaatagc gttgtcacaa aggatagaca caacggttag aaaatcaagt
     6601 agactcatcc cctctacctg aatctcctct ggaacttcga cattaaagac aatgttctta
     6661 tctctagctt ttagaaattt ccctgctagg agacttttga gggctttatc acgaatattc
     6721 accaatcgac caaggtcata tttattgtcc tgcaattttt cactagaatc ctttaagacc
     6781 gagtcgtaga tctcttttat ctgctccata tcctcctctt caatgcccag acgtaagcta
     6841 gtcaagaggt tggtgtagtc atggcgaaaa ctccgtactt ccttataaag ctcctctata
     6901 tgccgactat accgctccat ctctctgtag cgcaagtcct gctcttggtt cagtctctca
     6961 tggagtttgt ccttcaaata ggtatccaat ttcttgataa tccccataaa aaagagcagg
     7021 taaaagacta ggatgagatg gcgaacagtc gttgattgaa tcccctgttc atattcaaag
     7081 taagacagat tttgtatcac caaatagtaa gcccccatta tccagttaat ctgagtcagg
     7141 gacttttgaa aatctttatc gagaatcccc tttctcaagc tagtgaaatc atagtccaac
     7201 catttcaaaa aagctagaga aatgaagaaa ttgaaaatta ttatacataa ccaaataaat
     7261 gagtagtcat catatacttg cccttgtccc aaaaatggaa gcacaaaata ggaaacacct
     7321 ctataaaaga gattcactaa tatcattgga aagagaccat aaaagagaag gagtttttta
     7381 ggaagttctc ttaacaataa gaaagataag cctatgccgt acaagggttc cataaaataa
     7441 gatagataag tatttcctac catatagcca agcattccaa aaacaacggc caacagtatc
     7501 ttaaaaagaa aggccttaaa aatcctctca aaagtgagac taattccatc taccttaaag
     7561 aatatgacaa tttttaatcc gtgagtaaca agtgtataca acaatatcca agcaacattc
     7621 ataaactctc ctagctcagt gtaagttatt aatagcctca gacacttccc tgaccttata
     7681 acgcgcgatt agacaacttc caccattggg aaagaaaagc agtttttctt tcttatctaa
     7741 atgcaccaca ttggcaggat tgatgagaaa agagcgatgg cactgcaaga gacgaggctc
     7801 ctgcttgaga acctcctcta gactcgctgt aaattccagc ctgtctgtct tggtatagag
     7861 aataacacga tggggtcttg tggacgtttc gagatagtaa acctctttaa aaggatactg
     7921 gaattgagca aattttgatt taaagtaaaa gcaatcttcc gctagacttt tactgtcttg
     7981 accattggca tagaggaggg ctgtctcgat acgagattca aactcctctg ccgaaagggc
     8041 cttatcaata tagtccagag cagacacttg gtagcgaaag gacaggggca taaactccga
     8101 gtgagtcgtc acaaagacaa tcagggcata cggatcccga tcccgaatct ttctagccac
     8161 ttccagaccc ttcatctcct catttcgaat ctcaatatcc aaaaagaata gctgatgggc
     8221 ccccttctca tgcacctctg ccagcagttg gtctggcttg ccaaatacct caaaagagct
     8281 gggagtgata tgatgttcct tcaagagttt ctcaatcgtc gtttcaatcc tagtctgttg
     8341 ggaaaaatca tcctctaaaa caaatattct catcttctta ctctccctct ttcaaccatt
     8401 tttgacgaat ttctctatag cgacgtctgg catacttgac agcgtattga ccttcttctc
     8461 cgagaaaaag gattctttct tgaaaatcaa tcgattttaa tttatcaaga ttaaccaaac
     8521 aatttcgatg acatctcatc aaggtttccc catattggtt ctcaagatta cttaaatttt
     8581 gaagcatatt aaaactagct tcttctgtaa caatctgtac ggtatgggct ttagttggat
     8641 gtgtttggat atagtagata tcgtcgacgt ttactttata gactgttttc tgcgtctgaa
     8701 taatcatatg tttcattgga ttctcctcct aagaaaagaa taacagaaaa gttctagccc
     8761 aagcccaccc aatcctaaac agtctttgaa agctcctaaa aggattattt ttctacaaca
     8821 tcatgatgag cttacaacta ttttatcata aaattcactc agcgcaattc aagacatttc
     8881 aatgacaatt aaagatttga ataaaaaaaa agaattaaaa atgataaact aacagtatca
     8941 aaacacagtt gccagaagta agactggcac ccagattaaa ggaggcattc ccatgacaga
     9001 tacagacccc atcaaaagag ctcatacttt gattactgac ttaaacaaag cctaccaagc
     9061 atgcaaacag gcaagcgctg acgacgtccg ctttcaggag caattaaact ctattcttgg
     9121 ttttttagcc aaggctgaaa cagtggataa tcgattcttg attgaactag aaaaatttta
     9181 ccagacttcc agtcttctca tgggactcag cgctcttgat ccagatgctc caactcgcgc
     9241 cgcttggcgg gcctatgacc gcttccactt tgaccaagtc aagaccaag
//
LOCUS       DAVBHS010000082         8977 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVBHS010000082 DAVBHS010000000
VERSION     DAVBHS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8977
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.082"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(64..459)
                     /locus_tag="VQ651_001367"
     CDS             complement(64..459)
                     /locus_tag="VQ651_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET2754349.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(537..1298)
                     /locus_tag="VQ651_001368"
     CDS             complement(537..1298)
                     /locus_tag="VQ651_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET2754350.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(1331..1957)
                     /locus_tag="VQ651_001369"
     CDS             complement(1331..1957)
                     /locus_tag="VQ651_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HET2754351.1"
                     /translation="MIFTYNKEHVSDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(1973..2290)
                     /locus_tag="VQ651_001370"
     CDS             complement(1973..2290)
                     /locus_tag="VQ651_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET2754352.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(2287..2586)
                     /locus_tag="VQ651_001371"
     CDS             complement(2287..2586)
                     /locus_tag="VQ651_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HET2754353.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(2871..3062)
                     /locus_tag="VQ651_001372"
     CDS             complement(2871..3062)
                     /locus_tag="VQ651_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HET2754354.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(3223..3897)
                     /locus_tag="VQ651_001373"
     CDS             complement(3223..3897)
                     /locus_tag="VQ651_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754355.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            complement(3903..4349)
                     /locus_tag="VQ651_001374"
     CDS             complement(3903..4349)
                     /locus_tag="VQ651_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2754356.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(4451..4966)
                     /locus_tag="VQ651_001375"
     CDS             complement(4451..4966)
                     /locus_tag="VQ651_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET2754357.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVCVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLFLHVS"
     gene            complement(4963..5325)
                     /locus_tag="VQ651_001376"
     CDS             complement(4963..5325)
                     /locus_tag="VQ651_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HET2754358.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICSVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(5391..7250)
                     /locus_tag="VQ651_001377"
                     /pseudo
     CDS             complement(5391..7250)
                     /locus_tag="VQ651_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(7267..7737)
                     /locus_tag="VQ651_001378"
     CDS             complement(7267..7737)
                     /locus_tag="VQ651_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET2754359.1"
                     /translation="MDKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQANIKGIGGENKEISQKN"
     gene            complement(7861..8577)
                     /locus_tag="VQ651_001379"
     CDS             complement(7861..8577)
                     /locus_tag="VQ651_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2754360.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
BASE COUNT         2647 a         2098 c         1637 g         2595 t
ORIGIN      
        1 tcaaagcatt gctttgatgt tgtggataaa gctaacgtgg tttgaagaga ttttcgaaga
       61 gtattaaaat ggcaattctt cctcttccaa gactaaatct gccaaatctt ggcctgcatt
      121 attttcacgc atggcacgtt gagcacgact ttccaagagt tggaatcctg ttacaagtac
      181 ttcggtcacg tagttcattt ggccattttt ctcaaagcga cgggtacgca attctccatc
      241 aacggaaatg agactacctt tggttgcgta gcttgccaaa gtttctgcta gtctgcccca
      301 taggaccata ttgacaaaat cagcttcacg ttcaccgttt tggtctttgt aacgacggtt
      361 cacagcgata gttgctcgcg ctaccgactt gtcattgttg gttttgtgca attctggtgt
      421 agacgttaaa cgcccaatca tgataacttt attatacata ttttcttcct cctacttatc
      481 tattcgtagg aaatcaaaaa aagttacaga aatttgtaac ttttcgagga aatttcttat
      541 tttttatgaa tcatgaaacc tgtcgcctgt tgattggcca taatggtcat atctgtaatc
      601 tgaacacggc gaggctgact ggtcacatag actactgtgt ctgcaatatc ctgagcttgc
      661 aaggcttcta ttccttggta aacggacgca gctcgctctt tatcaccatg aaaacgaaca
      721 gttgagaaat ctgtttcgac aatcccaggc tgaatggttg tcaccttgat atccgttgcg
      781 atggtatcaa ttcgcagtcc atccgaaaag gtcttaaccg cagccttggt agctgagtaa
      841 acagcggcac cagcataggc gtagattcct gcggttgacc ccatattgat aatatgacct
      901 tgattgactt ttatcattgc tggcaagaaa cagcgagtaa ccgccatcag acctttaaca
      961 ttggtatcca acatggtcaa catatccaac tcctcatagt cttgataggg agccaagtca
     1021 agagccagtc cggcgttgtt gaccaagata tcaatcctcc ctatcgtttc tagaatatca
     1081 gagcaaacag tcttcaccat gaccatatcc gtcacatcta gtggaaaagt ccagacggtt
     1141 tgatttggaa agcttactgc aaactccgac ttgaggattt ctaatctgtc tatccgtcgt
     1201 cctgttagaa cgacatcctc accctgctcc agataagcac gcgcaatcgc ttcaccgatt
     1261 cctgaggttg ctcccgtaat cactacattt tttgccatct tatttccttc tatcagatgc
     1321 tgacaagttc ctaggcagtc cagtgtttcg ctgggtcgaa cggtgttccg acaacttggt
     1381 cttctgataa ttcaagcacc ccacgttttt gcggagcatt tggcaaatgc aattcacgag
     1441 gactacacat catcccaaaa ctcttttcac cacgaagttc gcctgggaaa atgaaattcc
     1501 cttttggcat catggcacca ggaagagcca caatggtttt caacccaaca cgcgcattag
     1561 gtgctcctgc aacgatttgc actatcttgt cacttgcgac tgcaacttgg cagatgttga
     1621 ggtggtcact gtctggatga gctaccatct cgacaatttc accaacaaca aacttaggtt
     1681 ccttatcatt aacaatttct tctgtaaaac cttccgcctg taattcttgg ttcaaacgag
     1741 cgacttgctc atctgataaa aagacttgac cgcgctctgc aatttcaaac aagctcgaaa
     1801 cctcaaaaat gttccaagcc actgtttccc cattttcttt gaggaaaaca cgggctacct
     1861 tgcctttgcg ttccacattc agtttggcat ctccgctatt tttcacgatg accataagga
     1921 catcactgac atgttctttg ttatacgtaa aaatcattgt ttcctttttc tcctatttca
     1981 atcctgctaa aaaatcgtta atttgctcct tacttttacg gtcgcgattg acaaaacgac
     2041 cgatttcctt gtccttttct agaacaacaa ggctaggaat tccgtaaaca tcccagagtt
     2101 tggccaaatc catatactga tctcggtcca ttcgaataaa ggtgaactct ggattggtct
     2161 cttcaatctc tggtaaggca ggataaatat aacgacaatc gccacaccag tctgccacaa
     2221 aaaggaagac cttcttgccc gctttttcca ctaaagatgc taattcttct aaacttgctg
     2281 gctgtatcat aagacttcct cctcatagac taggtcttca ttttcataga caaaggtata
     2341 atgacggcca tcctcaaaaa tgacgccacc aaccaagctc tccagactgc tttcgtaaac
     2401 ttgaacataa agggtcgcaa tttcccccat gtcggaaaaa tggtctcgca caatctctgt
     2461 caactcttcc tgagtcttca tgagcttacg gtcatctgca acttttttcg tagcaagagc
     2521 aaggcttccg atacctagca gagccaagcc tgccatccac atttttttag ctttcatacc
     2581 attcatttta acacaaaaaa ggcttcagga caaatgagga agcagcagaa aagcaagtaa
     2641 aaagcctctt cctttaagga aaaagacttc ttatactaag agcaaactag gaagctagcc
     2701 gcaggctgct caaaacacgg ttttgaggtt gtagataagg cgacgttgac gtggtttgaa
     2761 gagattttca aagagtatta ttcttattgc caggcaccta agttgccaac gtagtaacta
     2821 tcaggtgtgt aggtattgcg aagcatctta cctgatgaag ccagataata ctacttgcca
     2881 ttgtctttga cccaatcatt cgcaatcatg gaaccagaag aacttacata ataccattct
     2941 cccttgtcat aaacccaagt actgactttc atggttcctg agcaattaaa ggcaaaaaaa
     3001 ctgtccaata tcattcgttt tttaaaagca tttgacacta catcatctac gctgtagtac
     3061 atagatacat caaacgtaaa agccaaaata tcgaaaaata tacaactttt aaaggcagca
     3121 tttcattttt taaagtctaa caaccataga actgaaatca gagcaaggca tcaatttcct
     3181 actcttagta cgtctatttt gatggttgaa gtgaagattt ttttatttaa cgtagacaat
     3241 ttccaacttc ccaaaagcca catctccacg gatagtcaag gttttgctgg ttggggctac
     3301 aggagcgtca gccttagctg caccaaagga ggtttctact ttcaaatcta cacgccagtg
     3361 ttgtggaaca tagacggttg cattcccgaa ggccacttca atatttaaag ttgcaaaatc
     3421 acctagcatc tctgcattat catagtagat tttagcatcc ccaaaagcga cttccacttg
     3481 gtcatctacg agatcttgat cttgcttata gaaggtcccg ctaccaaaag cgacttcctt
     3541 atccgtgacg actgtttttt tcccattgta ccaccatttt ttttcattcc agaacttact
     3601 tgaatgcgtc agataaccaa caccaagtac caccaagatg ctagcccaaa taagagaatg
     3661 attggtaact ggtaacaagt cataagcgta atttgcaatg atgagcgcca gtaaacctgt
     3721 aaaaactgcc gaagtgagat gacgtctaag gatggactca atggacttat aagcaaaaaa
     3781 aacaatacct agtaaaggcc atattttacc atccaaagaa ggaattccaa aattcccttg
     3841 cagcaagatc caagctgcta aaaccaacaa aacaatacca aatgctttct ttttcatgtt
     3901 ttttacctca tgtttcttag attttctttt aggagggatt ggtaatgccg tgagacatga
     3961 acctccttgt gcgtctgata aaaggaaatg gtgcccgttc ctgaaaagga cttgtccact
     4021 gagtaaatct gacggatgtt tgcgatcgtt gacttggaaa ctcgattaaa atagcgaggc
     4081 aagatagact ccaactcata gagcttgagg cgaacctcat aagcttcctt ctggttatga
     4141 gcgtagatct tgctcccttc tgtttcaaag aagagaattt ctgacaggtc tagataatat
     4201 tcacctgtcc ccttgtaaaa agtcaacctc ggagcctttg actcttgcaa gagccgttgc
     4261 aagtctgcaa tttcatctgt caaagtgggg gtcttgatga caatttcagt ctcctctaaa
     4321 ttgccgtcaa tctcaattcg taacttcatc acatctcctt tctgactcta ctatatcaaa
     4381 gctaagttag gaatacaagg gcaaaaaagc aagtggtcaa tttgaagcag taagtggttg
     4441 taggactagc ttaacttaca tgtaagaata ggaagaaaag gaatccccac accaaaccac
     4501 aagcatagcc aaccaagaca tccttgggat aatgaacacc ccctaaaacc ctcaccaacc
     4561 ccagcaaagc tgaaaagatc aagcatacta agccaatagg tagactagca tgcagacagg
     4621 ccatggagat gatagttgcc gaaaagacat ggcgactggg catcgaccgt cccgaactat
     4681 ccttatcaag aaggggacag atatcccaag tttcataagg tctcgggtga ttgattttct
     4741 tacgaaacag ggacaaggcc acaaaacctg atgcaggaat aagcacacaa accccaactt
     4801 gctgaccaag tcctagttgc aggtaagtgg tgactagcaa gatcagatag accaggggca
     4861 tggcgactgt catcaaacga ttgaaactgc gtaataggaa taaaagaatt ggacgactag
     4921 ttagtctgga gctgatattt cgataccatt cttgataatt tttcatttat gcttctcata
     4981 cttttctaga acttgttgag atgttttccc cttggcaagc caatccacta gcttgtccaa
     5041 gcgacgtatt tcctgcatca gaggatcatc aatttcctca atacggacac tgcaaatctt
     5101 ccctgtaatt gcaattcgtt cagggacata atatggtgct tgtctaaaaa aatctccgta
     5161 agtaacatcc ctatccagac aagcaagaac atcactcact tcataccctg tcaaccaact
     5221 ggtcacttgg tgaactgact ctgcctttcc accctttcgc tcaacttttg caatcaaggc
     5281 taagtagaca gcagcaaatt tcatattgta taatttctga ctcatacttc tctctttcta
     5341 tgtgtactag tataaataag aaaaagaagg ccgtcaagcc ttcttttgat ttattcttct
     5401 gcttcatctt ctgtaaattg actattgtac aagtcagcgt agaagccacc ttgcgccatc
     5461 agttcctcat agttgccttg ctcgatgata tttccatctt tcatgaccaa gatcaagtct
     5521 gcatttcgga tggttgacaa gcggtgggca atgacaaagg atgtgcgtcc ttccatcaaa
     5581 cggtccatgg ctttttggat caattcctct gtccgtgtgt caacagaaga agtcgcctca
     5641 tccaaaatca aaagcggtgc atccttaaga agggcacgag caatagtcaa tagttgtttt
     5701 tgtcctacag acaaggtcac ggtgtcatcc aagatggtat catagccatc tggcaaggtc
     5761 ataataaagt ggtgaattcc cacagcctta ctagcttcca tcattcgttc atcactaatc
     5821 cctatttgat tatagatgag attgtctcga atagttcctt caaagagcca ggtatcctgc
     5881 aagaccattg aaaaggcatc atgcacttct gaacgcgtca tagccttggt atccacacca
     5941 tcaatgtgaa tacttccctt atcaatctca tagaatttca tcaaaagatt gacaatggtt
     6001 gtcttaccag tcccagtcgg cccaacaatg gcaacctttt gaccagcatg aactgtcgca
     6061 gagaagtcat agtcttgaac attgacaccg tccaccagaa tttctcctgc tgacacgtcg
     6121 tagaaacgtg gaatcagatt gaccagagtt gatttaccag aacctgttga cccaataaag
     6181 gccactgttt gaccagtttc tgctttaaag ctaacatgtt caataactgc ctccgaattt
     6241 gccgcatagc ggaaggtcac atccttaaac tcgacctgac ctttgaagtt ttcatcagtc
     6301 agctgcactt gaacagggtt ttggatagaa gaatgcaaat ctaaaacttg attaatccgc
     6361 ttagcagaga ccatagtttg gggaagaacg atgaagagtg ctcccatgag aaggaagtcc
     6421 atgacaacct acatggcata agacatgaaa acaatcatgt cactaaagag aggcagacgc
     6481 gctatcggag cagcgtcgtt aatcacatag gccccaatcc agtaaatcgc cacactcaaa
     6541 ccacttgaaa tccccatcat gataggattc aaaatagcca taagacggtt gacaaacaaa
     6601 tttaaacggg tcaattcatc atttactgct gcaaattttt cattttgata atcctctgca
     6661 ttgtaggcac gaacgacacg aatacctgtt aaactctcac gagtgatact gttcagttta
     6721 tctgtcagcc cctgaatcaa ggactgtttt ggaaaggcta gcgtcatcaa aacggtcgtc
     6781 atcaggacgt tgacaatcac tgccacaagt acggcccaga gccagtattc tgaatgacct
     6841 aaaatcttcc caatagccca gatagccata attggaccac acgttaccac ttgcaagtcc
     6901 atagtaatca acatttgaac ttgagtaatg tcattggtag tacgcatcaa gaggctagga
     6961 attgaaaatt tcttaatctc tgtctgcgag taatccaaaa ctcggttaaa aatatcactt
     7021 ctcagcctac tagtataaga agccgccact cgggatgcaa aaaatccaac tgcaactacg
     7081 gacaagaagg caagaaaggg cattcccatc atcatgcttg ccggctgcca caactcatct
     7141 aaattagttc cttgactacc tagcaaatcc gtaattttcg agatataggt cggcacttcc
     7201 aactctagat agaccgaaaa gcaagtaaag agaatggcta gtaaaatcat cccccattct
     7261 tttctactaa ttcttttgac taatttcttt attctctcct cctattccct tgatatttgc
     7321 ctgtagttga ccgagaactt tctcaaaaat cagtaattca tcttcatcaa tgtcttccat
     7381 caactgcttg tctatgcgtt caaaaaaagc cttaacctgt tgcatctgag aacgtgcttt
     7441 gtccgtcaga cgaacaaact tagcccgctt atcaacagga ctcgcctcca attccaccaa
     7501 accattttgc actatacgct taaccagatt actagcaaca gacttggtaa tattgagttc
     7561 ctgctcgata tctttaatca agaccaagtc ttggtttttc tcgcgattat ccaaaaaatg
     7621 cacaacctga ccttgcggcc cacccataaa ttcaatgccg caacgtttgg cttccttttg
     7681 caccatcagg tgaatttgat gaccaaaacg cttaaagact aacatcggtt tatccataat
     7741 ctcccccttc taaataaaaa tagttctctg gagaataatt aaatttctat gagaactatt
     7801 ttcttgatta aaaaaatccc aagtgatttt ctcacttagg atcatgttct ataggttaaa
     7861 ttaaaaccca tctacgttcg tataaatctt ttggacgtct tcgtcgtctt caagaacgct
     7921 gtaaagtttt tcaaaggttt caaggtcttc gcctgacaat tccacttctg actgaggaat
     7981 catttccaat tcagtcactt ggaattcttc aataccagac tcacggaggg caacgatagc
     8041 cttgtgaagg tcagttggcg ctgtgtaaac tgtgattgta ccttcttgtg cttctacgtc
     8101 atccacatcc acatccgctt cgagcaattg ctcaaagact gcgtccgcat cttcacctcc
     8161 aaatacaata acacctttgt tgtcaaagag gtaagaaaca gaacctgaag cgcccatgtt
     8221 tccgccgttt ttaccaaagg ctgcacggac attggctgct gtacggttga cgttagaagt
     8281 caaagtatcc acaattagca tagagccatt tggcccaaaa ccttcgtaac gtccttctgt
     8341 aaaggtttcg tctgtgtttc ctttggcttt atcaatcgct ttatcgataa tgtgttttgg
     8401 cacttgggct tgtttagcac ggtcgataac gaatttcaaa gctgagtttg attctggatc
     8461 tggatcacct tttttagctg ctacatagat ttctacacca aattttgcat atactttaga
     8521 gttggctcca tctttagccg ttttcttggc tacgatattg gcccatttac gtcccattag
     8581 gaatctcctt ttttcacatt ttaatctttc ttattataac acaagttttt ttgattttca
     8641 ctagaggaaa tggattttat tagcaaatca agctaggata gcactttacc tgctaagatg
     8701 gtcttgcctt tctatcttta tcaacagaca ctcatccaca ttcaaaaaac aaactagacc
     8761 attatctgca aatagaaagt ttcagccaag tttgacaaag tcagctcaaa ttactgtttg
     8821 aagtttgtag atataagcga caaaaacaat catactgcac cttttgttga cagtctactc
     8881 cagacatatc atagttcaag taaatacttt gaaatccaac agttcttata ggcgctattg
     8941 tattctaaga aatcaataga agagtttcta agcaaac
//
LOCUS       DAVBHS010000083         8932 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVBHS010000083 DAVBHS010000000
VERSION     DAVBHS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.083"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(149..952)
                     /locus_tag="VQ651_001380"
     CDS             complement(149..952)
                     /locus_tag="VQ651_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HET2754361.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1231..1986)
                     /locus_tag="VQ651_001381"
     CDS             complement(1231..1986)
                     /locus_tag="VQ651_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HET2754362.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(2056..4707)
                     /locus_tag="VQ651_001382"
     CDS             complement(2056..4707)
                     /locus_tag="VQ651_001382"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET2754363.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYSTTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPKFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTDDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNEAGEIYVGEEAPAGDGWKQDEDVLDTWFSS
                     ALWPFSTMGWPNTETTDFKRYYPNSTLVTGYDIIPFWVSRMIFQGIEFTGKSPFKNAL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIDKYGTDSLRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAEYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(4700..5278)
                     /locus_tag="VQ651_001383"
     CDS             complement(4700..5278)
                     /locus_tag="VQ651_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET2754364.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYQLMYLKQIQNLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(5278..5748)
                     /locus_tag="VQ651_001384"
     CDS             complement(5278..5748)
                     /locus_tag="VQ651_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2754365.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGYG
                     IVVKGTDKIVGSVDFNHRHEDDVLEIGYTLHPDYWGRGYVPEAARALIDLAFKDLGLH
                     KIELTCFGYNLQSQRVAEKLGFTLEARIRDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            complement(5735..6313)
                     /locus_tag="VQ651_001385"
     CDS             complement(5735..6313)
                     /locus_tag="VQ651_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET2754366.1"
                     /translation="MKQSFKTNKLYYGFPVFILGYQDQNFGYNITTCSSSYSLGDWVV
                     IGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEKLERLGVHYEISERTQ
                     TPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDEKGHFKNQLFAPTYFM
                     GDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(6596..6991)
                     /locus_tag="VQ651_001386"
     CDS             complement(6596..6991)
                     /locus_tag="VQ651_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET2754367.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(7087..7341)
                     /locus_tag="VQ651_001387"
     CDS             complement(7087..7341)
                     /locus_tag="VQ651_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HET2754368.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            complement(7350..8684)
                     /locus_tag="VQ651_001388"
     CDS             complement(7350..8684)
                     /locus_tag="VQ651_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HET2754369.1"
                     /translation="MTDEWIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVLDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTVKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKRLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(8684..8917)
                     /locus_tag="VQ651_001389"
     CDS             complement(8684..8917)
                     /locus_tag="VQ651_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HET2754370.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLTGTPMDKIVRTLEANGYEVIGLD"
BASE COUNT         2516 a         2008 c         1695 g         2713 t
ORIGIN      
        1 cttgtgcaat acagaactaa atccttcgga taaattattt gtctaacttt ttggggtcag
       61 tacacctaaa actttgatga tatacgtttt cttgtgagaa tatttacttc atttttgcct
      121 aaaattcaat gtttactcag tatttggatt atgaaaaatc gaggtctaaa tctagataca
      181 ttttttctga agacaaatca ttttgaccac cgagcaagag attttcaaaa aaagctgtta
      241 aaaactcaga acgtcgctgt aaaatctttg cattatctaa taccaaggca tcacgaaaat
      301 atttggaatg ttgctgaaat ggtgtattat caatatcaaa accaaactca cgaagatact
      361 gaatcaaaaa gaccgttact gtccgagtgt ttccttcgcg aaatggatga atctgccaga
      421 ttcctgaaat aaaatgctgg atttgtttaa ccacatccgc ctgagttagt gtcgcatatg
      481 caacttgttt ttcctgatta aaatcataat ctaaggtcat ttgaatcatg gagtaatcag
      541 agtacacaac actttcacca ttcaaaacag gttcattctt tgtgatattg gtctgacgaa
      601 attgccccac cggaatagag ggttcaaata tatcttgaaa caactcctta tgaatagcaa
      661 gtaaggtcgc aggactaaag ctaaagcctc ttcgagacaa tagttctaca atacgtagag
      721 aaaccaagtc tgcctcttcc gtacttgcat caatagtatg gtgataagcc gttgcatcct
      781 cataaacctg ctcataagtc agttctcccc gggactgttt ctcagccaaa gattccatat
      841 acgctgatgg cactagattg tcaactttct gcagaccaaa acctatccgc cataaatcac
      901 gcttcgcttc ataagacaag tttggattgt caatgttgta agttgattgc atagaaatat
      961 cctttcaatt gtttttcttc attaccagta aatcacgaaa ttggttggaa actggcttaa
     1021 actataaaaa gtcctcacaa taggttctat gatatatata tatatacttc atgtaaataa
     1081 taaaagagtt ataatcagca ctactgataa attcaatacc gatttcgcca tcttaaaact
     1141 tagtcaaacg aataccgttg atttccagtt cattaagtcg ttgacctgtc aagacttctc
     1201 gttccttcaa ctggaaaagt taagggggaa ctaatacaaa tcctctacgc ttctatattc
     1261 aatgatttca tttttgataa tattcttttt aaactcaaaa tgttttaaag gattatcgat
     1321 taacactaac tttggaatat catctagatt tgtgaccaag gataaaataa aatccgattg
     1381 aaactccttg actttttcaa attcttttgc agttaaacgc acacgcccct tagattttcg
     1441 gatacctttt acttcggcaa ggtaagaata ctcttggaca tctacctgaa agtcatatcc
     1501 atcaccataa agacgggcat cggttaactg tccaccttgg aatttttctt ctttatcaaa
     1561 atgaaggata aaataattct cagcctccat tcccgtttcc tgtagacgtt tgaatttagt
     1621 tcgaactatt ggtttttcaa cgatggttat gcctgttttc ttcccttcac tggcaagtaa
     1681 cattttaact atttcagcat aactatgaac atcttcattt ccaaacatca tatcaattaa
     1741 atctttacga aaacgataaa cttcagcttt ctgccaccaa ccttttcgat tattgtcaaa
     1801 ataaggatca aataaatcca ttctattttt tacaactcca gttgtttcag caattcccag
     1861 agaaaccaca tagcgataaa actccgactt gctagagcat tgaaattctt ttataaaaag
     1921 cttgtcaaac ttggcaagac cgtagccaat taaatttaac aattcataat gagggtgttt
     1981 ggacatcact ttctccatca atagatattg atttcattat accaaaaaac acggctcatc
     2041 accgtgtttc tgtgtttatt tcaccaactt cttcatctca tcaatacgtg ctacggtcgc
     2101 atcatactta gcttggtaat ctgcttgctt gtcgcgttct ttttggacaa cttctggttt
     2161 agcgttggcg acgaagcgtt cgttgctgag ttttttgcca accatgtcga gttctttctg
     2221 ccatttggca agttcttttt ccagacgtgc caattcttct tcgacattca agaggtctac
     2281 cagtggcaag aagatttcgg ctcctgtgat gacgcttgac atagccagtt caggtgcagg
     2341 gatggttgat gcgatttcca agtgttcagg atttgtgaaa cgtttgatgt agttgacatt
     2401 gctgttaaag aaggcttcca agtcgctatc gcttgtctta acaaggatag tgataggctt
     2461 gcttggagca acgtttactt ccgcacgcgc attacgaacg gcacggatca agtctttaag
     2521 gctttccaca ccagtgtgag cagcaaggtc ttcaaaggct gggttgacag ttgggtattc
     2581 tgctgtaacg atagatcctt ctgagatttg tccaaagatt tcctctgtca caaatggcat
     2641 gattgggtga aggagacgaa ggatcttgtc caaagtgtat agtagaacag aacgtgtgat
     2701 gactttctct tcttcgttat cgctataaag gacttccttg gtcaactcaa cataccagtt
     2761 ggcaaattct tcccagatga agttgtaaag gatatgacca gccacaccaa actcgaactt
     2821 atcaaagttt tcagtaactt ttgcaatggt ttcgttgaga ttgtggagaa tccagcggtc
     2881 cgtcacatta ccagcctcac ctgttgcaac ttttgtgaca ttgtcatgcg ccacatccag
     2941 cgtcaaacct tcattgttca tgaggatata gcgagaaatg ttccaaattt tgttaataaa
     3001 gttccatgaa gcatccattt tctcgtaaga gaaacgaacg tcttgaccag gagctgagcc
     3061 gtttgacaag aaccaacgaa gactatctgt accatattta tcaataacat ccattgggtc
     3121 aatgccattt ccaagagatt tagacatctt acgtccttct tcatcacgaa ttagaccatg
     3181 gataagagca tttttaaatg ggctctttcc tgtaaattca attccttgga aaatcatgcg
     3241 tgatacccag aaaggaataa tatcatagcc tgttacgagt gttgaatttg gatagtagcg
     3301 tttaaagtca gtagtttcgg tattaggcca gcccatggtt gagaagggcc agagggcaga
     3361 gctgaaccaa gtgtcaagga catcttcgtc ctgtttccag ccatcgcccg ctggggcttc
     3421 ctcgccgaca tagatttcgc ctgcttcatt ataccaagca gggatttggt gaccccacca
     3481 gagctgacgg gaaatcaccc agtcgtggac attttccatc cattgcagga aggtgtcgtt
     3541 gaagcgcggt gggtaaaatt ccaccttgtc atctgtgtct tggttggcaa tggcgttctt
     3601 ggccaattgg tccatcttga cgaaccattg agtagacaag cgtggctcaa ctacgacacc
     3661 tgtccgttct gagtgaccga ctgaatggac acgtttttcg attttgacaa gggcaccgat
     3721 ttcttccaac ttagcaacga ctgccttacg agcttcaaaa cgatccatgc ctgaaaattc
     3781 aaaggcaagc tcgttcatag ttccgtcgtc gttcatgacg ttgacttgtg gcaagttatg
     3841 acgttggcca accaagaagt catttggatc gtgggcaggt gtgattttca cgacaccagt
     3901 accaaactta ggatctgcgt gctcatcacc aacgattggg attaatttat tagcaattgg
     3961 aaggatgaca tttttaccaa tcaagtcctt gtagcgcggg tcttctgggt tgaccgcaac
     4021 cgcaacgtcc ccaaacatgg tctcaggacg agttgtagca acttcaaggg cgcgtgaacc
     4081 atcttccagc atgtaattca tgtgatagaa ggcaccttcg acgtccttgt gaataacctc
     4141 gatatcagaa agggctgtgc gagctgctgg gtcccagttg atgataaact caccacggta
     4201 gatccagcct ttcttgtaaa ggtccacaaa gaccttacga acagcttttg acaaaccttc
     4261 gtcaagagtg aaacgctcac gagagtagtc tacagagagc cccatcttgc cccattgttc
     4321 cttgatagta gtggaatatt cgtctttcca ttcccagact ttcgtcaaga aagactcacg
     4381 accaaggtca tagcgggaaa tgccctcacc acgcaagcgc tcctctacct tagcctgagt
     4441 ggcaatccct gcgtggtcca tcccaggaag ccaaagggta tcaaaacctt gcatgcgttt
     4501 ttgacggatg ataatatctt gcaaagttgt atcccaagcg tgaccaaggt gaagtttacc
     4561 tgtaacgttt ggtggtggaa taacgattga ataaggctta gccttttgat cgcctgaagg
     4621 cttgaaaaca tcagcatcaa gccatttttg gtaacgacca gcctcaacct cggctggatt
     4681 gtatttagat gaaagttctt tagacatgtg tgtgtccttt ctctattttg tttattttat
     4741 tttgaatttg cttagcagct tcttctgcag acaaattcgt attatttatt ttaaagtagt
     4801 ggtgcaactc attcggttga tattgggaat ttaattgaag tgtttcagcg gtctctaaaa
     4861 tttctctttc agatacctca atatgtcgtt ttaagggttt gtgctttaat cgattctccg
     4921 ttcgatttcg acgtatgcac tcttcaagac ttgtttccaa ctcaacaaaa agaatctctt
     4981 gatgataatc atccaacaaa ttctgaattt gctttaaata catcagttga tactgatttg
     5041 aaaaatcaat tacatctgtt aaaattactg atcgctgatt tcttgcactt gctccaagga
     5101 atgaaaaagt gattccacga acaaattccc acatttcctc tgtatagtcc tgatagattt
     5161 ctagtgcaaa atcaatggct tgatggttat aaaatagggt agcatccgtc agtcgagata
     5221 attcttgacc aatggtcatt tttcctgatg ctggagcacc aatgatgaaa agatgcatca
     5281 aatcacctcc cactcactct tcagcaaggc atatctcaaa tcatcacagc agttgccttg
     5341 agcatcttta cggtctctta tgcgagcttc gagggtaaag ccaagctttt ccgcgactcg
     5401 ttgactttga aggttatatc caaagcaagt tagttcaatc ttgtgaagac ccaaatcttt
     5461 aaaggctaag tcaatcaagg cacgcgcagc ttctggcaca taacctcgcc cccaatagtc
     5521 tgggtgtaag gtatagccga tttccagcac atcatcttca tggcgatggt tgaaatcgac
     5581 agagccaacg atcttatccg ttcctttgac gacaatcccg tagcctgctg gataggcgac
     5641 ctctggcaaa ctagcatagt caaagatatc ctcagcatcc gccacagtac ggactcgtaa
     5701 aacgagacgt tctgtttcga ttttatctgg cagctcagtt cttgccatcc ttcttcctcg
     5761 cttttttgat gaaactgccc ttcatatcta cacgcttatc cagatagcga taaacgcgct
     5821 gatatccatc tcccatgaaa taggttgggg caaacagttg atttttaaaa tgtccctttt
     5881 catccaggag ttctggggca acaagtcgct caagaatctt ggcaaagatg tggcaaatac
     5941 cgtcttcctc aacaatccta tctacccgac aatctaaaac aagtggacag gcgtctaaaa
     6001 taggagtctg agttcgttca gaaatttcat aatgcactcc caaacgttcc aatttctcct
     6061 gatgactgat aaaaccagcc tgctccatcg caagcataga agtttcatca gaaatattca
     6121 cagtaaattt ttgatactgt ttgatctgct ctgcggcatt ctctctcgca acgactccaa
     6181 tcacaaccca atctcctaga ctatacgagg aactacaggt cgtgatgtta tagccaaaat
     6241 tctgatcttg atatcctaaa ataaaaacag gaaaaccata atatagttta ttagttttaa
     6301 atgattgttt tataacaacc tcctttgact aagacacaaa agaaaagccc tgccatctac
     6361 atgacaggga cgaatgtatt tatccgcggt accacccaat ttcgggccaa gcccgcatct
     6421 ttagtcttta aaataaaaag acagaaatat ttcatttttt cagtcattag caaccagttt
     6481 tgacacattt gtggacttct cagcgccgcc actttctgta aaatgtggga ttcaaaacac
     6541 ctctaatagc tctattgtag caagtttttc tcggaaatgc aaggctcaag aaaaattact
     6601 tgaacttgat gccattttct ttcaatttct tgatggtagc tgggccgatt cctttcaagg
     6661 caaggagttc tttttcagtc cagtttttga aatctgaagc agacttgatt ccttcatcat
     6721 agaaagtctt ggcacgatca agtggaagtc cacccaagtt tgctgcaaaa tcttctacag
     6781 aattagctac tttttgagct tgctctttgg tagcttctac tgcttgttca acttttttag
     6841 aaacagcctt accagctgcg cttgctgact gttctgcacg cttcacgact ttttttgtct
     6901 catctacaac cttagtcaca gtacttgaaa aggcacctgc gcgacggagg ccatttcgta
     6961 attgtttttt acgattgagt ttctttgaca tgataaaatc ctttcaataa aaaatccctg
     7021 ctctgacaag gatttaacat aaacattcta tcaagatttt agtgaaaaat caagaatctt
     7081 tctcgtttaa taatttcgct caccaaacaa gccttctggc aaatcatgga atcccttgcc
     7141 tactttgaag ttttcaaact taccaagcaa gaactgataa gcatccaagc gactctcgta
     7201 gcgaatcatg gcatctttgg cacgctcgtc ctggtcttct tcgtaaactt tttgactttc
     7261 cttgtgcgcc atgtcgttaa tcatgatttg ggtattgacc caagcttcaa attccttcat
     7321 aaattcttgt tcgtaactca tttttctcct tattctaatc cacgaaaata gtccgtatca
     7381 atctcacggt agggctggat atcctgaaca tactccaaca ctccttggaa ttctccgtct
     7441 tcatcgtgta ctgcagcata ggtgatgtgg acaaacttac ctcgcgactc agacttgaac
     7501 cacatttcat acttgtcttt gcttccctca cgaagcctct tcatgatagt tttgaccttg
     7561 tccaagtact taggcggatg gcagagttcg acattgcgcc cgacttgaga cggcgtccgt
     7621 ttgaaaatca tctcatcagc tggcgtattg tcattgtaat actggaaaat atcttcttta
     7681 ttgacaaagg taatctccat agggagatga ttgaggatga gattggcctg ctcgactgaa
     7741 agatagccat taccaaaagc ctgttgacta tggcggtcca gcacagcttc cttttcctta
     7801 ggggtaaagg taatggtaaa atggccttct ggcgtatcta tgacttgttg aacttgacct
     7861 tctgccgtat ctagctgtac aggctcctct gcaatctttt cctcaataaa gctctgtcgt
     7921 tctggcaccc atttctctga cggacggatg atggcatagc cataggcatc gctctcctcc
     7981 gcaatctgaa gccagtcatc ctgagtaaaa gactcaagga gaatcatgag gaggatggac
     8041 tcttccttga aaatcatact ttcaaactct gtcgcaaaag cttcaaaagc ttcctttaca
     8101 ctgctaattg acacttctgg tagtgacttg actgtcgtta gagctgtttg aaagagttcc
     8161 ctaatctgat catccactcc ccacataact ttgggaggtg aatcgtgtcc atagcgctcc
     8221 atgataggaa agaagagttc ttccttacgt tggtaatgga tgtcaaattg ccccacaagc
     8281 cccatctgac gcaccaagcc cttgcgcatc tccgcaagca tttcctcgtc ttccatcaac
     8341 tcataggtat ctaacaatct acgaatgcga atcaaagctg cacggagggc caaattttct
     8401 tccttaaaga cacgaactgg gtgcccaggg tgttcggtat ctaaaacttc gacacctttg
     8461 atagcatttt taaaaagatt ggcatggaca tcacagagtt ccataacatc ttcaaaagtg
     8521 acgcccgaat ccgagttcat cagctcgtgc tccataaggg aaatctcgat ggctgacacg
     8581 cccgtaaagg tcgcatcaaa gcgatcttga accgactcag gagaggcgcc attgtgcaat
     8641 tctaacaaaa tatcccgtag gatatgaatc cattcatctg tcattagtct aatccaatca
     8701 cttcgtagcc attcgcttcc agtgtgcgta caatcttgtc cataggagtt cctgttagct
     8761 tagaaccctg tttaagtgat actttacgac caactgtatt gcgcattaag ggattggcaa
     8821 ggggtttaaa acccaactcc actagaattt ccaagacttc tggatgcttg tccaccactt
     8881 ctgcaacagg aattgacaca tcgatgatat tgtccatgac gacctccatt gt
//
LOCUS       DAVBHS010000084         8915 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVBHS010000084 DAVBHS010000000
VERSION     DAVBHS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8915
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.084"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(180..1028)
                     /gene="dprA"
                     /locus_tag="VQ651_001390"
     CDS             complement(180..1028)
                     /gene="dprA"
                     /locus_tag="VQ651_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HET2754371.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1126..1815)
                     /locus_tag="VQ651_001391"
     CDS             complement(1126..1815)
                     /locus_tag="VQ651_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET2754372.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQNIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     HKLEEILKNEN"
     gene            complement(1827..2705)
                     /locus_tag="VQ651_001392"
     CDS             complement(1827..2705)
                     /locus_tag="VQ651_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET2754373.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2695..3564)
                     /gene="licA"
                     /locus_tag="VQ651_001393"
     CDS             complement(2695..3564)
                     /gene="licA"
                     /locus_tag="VQ651_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HET2754374.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3581..4603)
                     /locus_tag="VQ651_001394"
     CDS             complement(3581..4603)
                     /locus_tag="VQ651_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET2754375.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4608..5315)
                     /locus_tag="VQ651_001395"
     CDS             complement(4608..5315)
                     /locus_tag="VQ651_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET2754376.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDSIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5651..7138
                     /gene="tacF"
                     /locus_tag="VQ651_001396"
     CDS             5651..7138
                     /gene="tacF"
                     /locus_tag="VQ651_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HET2754377.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGDILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7148..7951
                     /locus_tag="VQ651_001397"
     CDS             7148..7951
                     /locus_tag="VQ651_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2754378.1"
                     /translation="MKQLTVEDAKQIELEILNYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRLVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSATFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            7953..8762
                     /locus_tag="VQ651_001398"
     CDS             7953..8762
                     /locus_tag="VQ651_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2754379.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDQFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         2801 a         1793 c         1493 g         2828 t
ORIGIN      
        1 gtttgaagag attttcgaag agtattatat gagaaaaatc ctctactcta ctaatccact
       61 tagaaaaaac gcatattatc aagtgttaaa aacttgctaa tatgcgtttt attatggaaa
      121 tgttgggttc attttctctt aaaattgatt ttttcttaga attctagctt aggtcatttt
      181 taaaattcaa attccgcaag aacatcttgc ccactggtga ccaattttgc tccttcttga
      241 atcaaatgat ggcaaccgtc tgatagtcca tctaaaatgc taccaggaat agcaaagaca
      301 tcgcgtcctt cttccattgc tcgctcacac gtaatgagac tacctgaacg catcttagcc
      361 tctgctacaa tcacaccacg acaaagtcca gcaatgatgc gattacgggc aggaaaatga
      421 aatttcagag gttgttcgcc aggtccatat tcacttagaa ccagatggtc attgccgatg
      481 tagtcttgca agcgtttatt ggctttagga taaaacacat ccagtcctgt tccaatcact
      541 gcaatggttt ttccgccatt ctgaagagct gccatatgag ctgctgtgtc aatgcccttg
      601 gccagaccac tgacaataac cagttcattt tccaagcctt gaatgacttt ttcaactgat
      661 ttagctccct gtttgctaca agcacgactg cccacgaccg ctaccttcgg gaatttcagg
      721 aggtcaagat ttcccttgta aaataaaagt acaggcgcat catatatttc actcaaatcc
      781 caaggataac agtcatctaa aatagagaaa gatggaaatt tttgaaactc tttcgacaaa
      841 tgcgcatcgt ctatctgaaa ataacgttcc ataaaaacgg ctggattacg gcaacctgat
      901 atatctgcaa tatcacccaa caaaagctcc tgatcaacat tttcaccgta ttctagaact
      961 ttcaaaatct gttgattggt caaacctgat ttttttaact tatagatttc atagtttgtg
     1021 attttcataa ataactccat ttctttttct actcatctat ctattcgtaa aagttcgtaa
     1081 aaaatcaaaa aaacatccga ctattttgtc agatgttgga agcttttaat tttcgttttt
     1141 aagaatttct tctaatttat gatagtcttg gacactatca atctcataaa tgctattgcc
     1201 ttctaattct tcaacataga catctagctc tttgatgtta tccttaacca tattgtccca
     1261 atagagatca acaaattcgc cgcttgcata agccttatcg ataaagctaa caattttttc
     1321 tgctgttgga gcatcccaga aggatacacc actaaggatg cgacctgcct tgctatcaac
     1381 aataatgttt tgaaccttgt agtcatctcc atagaccaag aaccattcgt tggtacaatc
     1441 ttcacgatag acactaaaat aagtagaacg tgtcaaatca ttgcggaaca tatttttaaa
     1501 gagatagtta tcggcatcga taacataact attggctaat tcttctttta caagatagag
     1561 agagtaaaag ttattgtagt cagcgtattt atcattgaaa acgagacgaa caccgtattt
     1621 ctctttcaag taatcgaatt gttctttaag ataaccaaca atgatgatga tgtcattgat
     1681 tcctttttct ttgagaaact caatttggta ctcaatcaaa ggtttttgat taacctgaac
     1741 caaggcttta ggggtatttt cagtcatagg acgcaagcga gttcccaatc ccgctgctaa
     1801 gatgatggct ttcacacgaa tctcctttat tctttaataa taatataaac tccagcaatg
     1861 acgacaagtg acgtcataat ggtcagcata tctagcggtg cacccaagaa aacaactgca
     1921 aacaagaccg tccatactac atagctcacg ttcaagcctg tagccttggc tggttgcaag
     1981 cgattgatag cgatataata agccaagtag gaaatcatat caaaggctgc aaaaacaatc
     2041 atgagaccta gcaattgtcc attggctact gcagtaaatg actgatgaga gaagagcaca
     2101 atcacaagat aggacaagaa cgaagttact tgacggatta agagggcttc gatttcactc
     2161 aattcacttt ccatggcaaa agagctaaga acactctcac ttccccatgc aatagcacaa
     2221 accaaagcac aaagaatccc aatgtagaaa gaattaacct gttcaacttt ataggtctgg
     2281 gcaataatcc ctccaataat caagacaatc ccaaatacag tatttttcga aatcttgtgc
     2341 ttcaaaaaga agaaagccaa tagaactgaa atcgcagggt aaatagccga tacagatgaa
     2401 gctaaagaac ttccgatata cttaactgca taaagattgg cctgcatacc gatagggcct
     2461 gctagcaagg ctccgatgat aacactgaca ttgcgaatat ttaagaaaat tgagaggcga
     2521 actttccctt cttttaccaa gagaaaagct agtaagataa agatgctcaa aaaatcatga
     2581 gctgcagcca ccacaaaggg tgacaaatct gtaaaaatcg aaaagatata agcactaacc
     2641 gttagaccca agccccagaa aatacctgag agaaggccaa aaggaactcc gtttttactt
     2701 ttcatctgaa cctccataag aagccaaacc tttaacagct ctttggtaac gattcacacc
     2761 atagtcacca aaatcttcac cttgctcttc cttatagaca gtccatagac tccaaatagt
     2821 atcttgtaaa attttataaa tagcaatctt ttcatgagaa accggtgttt ggtcactctc
     2881 atagtgagat aagaaagttt cttcctcttg ggaagtgaat tcagactcta aaaagagggc
     2941 agccaaatcc cacattggat cattcattga tgaatattcc cagtcaatca aataaagtcg
     3001 tccttgaggt gattcgataa agttttcagg caccaaatcg atatgacaag attttctgtc
     3061 aacacctaag tcagccagtc ttttctctaa ggagaagact gcatttctaa cagattcata
     3121 gttggcataa ggaatttgtt cttcaatcaa ggattcgtat tttttgattt cttcaaaagg
     3181 agcaaattct cctcttaatt ccttagcaga cgtatgaata gtttgtaata ttggagcaat
     3241 tttgtcgaac ttggtcttga ttgacgttga atcaagcgta atcgcagatt cgatatactc
     3301 atttactttg ataccagctt caatatcaaa aagataattt tttacatcta agcctaaatc
     3361 ctttagtagt tcaagattgt acttttcatc ttgtcgattg ataagctttt ctgtcccttt
     3421 accaaagaat ttaacaatgt attgcttatt tgttgttttg gccaaatagt tttgattggt
     3481 cattccaccc agttgttcaa cactgaggac ttcctcttct tgactaagta aggaagaaat
     3541 tttttcttta atgattttct ccacaattaa cctccagtac ttatacttcc cacttaaaca
     3601 ctgttttaaa ggctgtgttt aaatcggttg caaagacacg atgaatatct ttaatttctc
     3661 ttacaggttc ttctagataa aggatatttt taagacgatt ggcaaatttc ttgacttcca
     3721 tcatttggat agcattttta aaatcaatgc gaccagaacg agatgaccca accaaaatca
     3781 agcccttttc taaggcatcg cgagtattga gattgacttt atattcgcta actcccatca
     3841 tgagaatcgt tccctgagga cgaatgtagc gaatcaagtc attaatagct ggtccagtac
     3901 catcaccacc acaacattca aaagcatggt caaaggccaa ctcttcagga atattatccg
     3961 taatatagca ttctttggca aatgagaaga gttccaactt ttcccaatga cgaccaataa
     4021 ccacaatctc tgcttctggc aaagtatagt tgataatatt ggcaaccaca aaagctaaac
     4081 ttccatctcc aataacggcg atccgctccc gcttgctatg agcaagagtc aatagacgat
     4141 tcatagcgtg catgcccaca ctgacaaact ctgtaatggc tgcaaccgta tcttcaatag
     4201 catcataagc caccacacga tctttaggga gagaaacaaa ctctctcata aagccatcaa
     4261 atccactaga caagaaatgg gtccctgtca tgtagttttc atagaattct tcatcactct
     4321 gcataggaga ctgattggga atcatgacaa ctttttgacc aacctcgtag gttccggtcg
     4381 ggtcagaaat gacggttcca catgactcgt gaatcattgc cattggaagc tttttattca
     4441 aaatcttggg atcacgtttt ccctgatagt aacgctgatc cgcatgacag acagccatgt
     4501 agttgggacg gataaggata tgattctctt ggtcaatagc ctcttcctga tatttgacat
     4561 tgataaactt aggcttagtt agttgataaa tttgattaat cattttacta gtctttctca
     4621 atcatacttt ttgcaatctt caaatctgtt acggttgtaa tcttcagatt tgagtattca
     4681 cctttggcca aggccacatc ttttcctttg atcacaaaga ttttacatgc atctgtcaag
     4741 atttccttct cttcatcaga aagagatcca taaaggtcca tgaagtcctt gcaacggaat
     4801 gtttgaggtg tttgtccttg ataaaggtga gcacgatttg gaatatctgt aataaattga
     4861 ccattggtac tttcaacgat agtatcaacc gcttctacca ctgtgtccac tgcgtcatga
     4921 ttttgggcaa gttggatact gtcctgaatc atgcgaagtg taataaatgg acgaacagaa
     4981 tcgtgggtaa caacgatatc ctctggagta agcggacgat aagcatcaat ggcttcaatg
     5041 attttcttaa tacttgtatt gcggtcagca ccaccctttg taatgatgat acgttcctta
     5101 taaagaggaa gatatttatc tacaagatct tctgcatgag aaacccagtc tccatgaaca
     5161 ccaactacaa ttttttcaat acttggctcc aagacaaatt tttcaattgt atgaatcaaa
     5221 ataggtcgat cacctagctc taaaaattgt tttggcaagt tactgatccc catgcgtgtg
     5281 ccagttccac cggcaagaat tcctgcataa atcatctatt ttctcctttg tcttactaaa
     5341 aaaacttatt ctctctatta taccacaatc tagtcctatc ataaaagaaa tactggtcct
     5401 ccccttctag aataggagaa ttatgcagtt ctattattca aagaaactag tccatttcta
     5461 gtaatgacta ggaattctgt agcaattact aataaatggc tgcgaaattt tagagtcctt
     5521 cagaataata tactttcctt aaagaaaact taaaacttaa gatttgaaac ttcaaagtga
     5581 ttacttgact tactgccctt catttcttat tagctaactt tatgatataa tgaaaaacga
     5641 ggtaaattga atgaaaagta taaaattaaa tgctctatct tacatgggaa ttcgtgtctt
     5701 gaatattatt tttcccatcc taactggaac ctatgtcgcg cgtgtcttgg accgaactga
     5761 ctatggttac ttcaactcag tcgacactat tttgtcattt ttcttgccct ttgcaactta
     5821 tggtgtctat aactacggtt taagggctat cagtaatgtc aaggataaca aaaaagatct
     5881 taacagaacc ttttctagtc ttttttattt gtgcatcgct tgtacgattt tgaccactgc
     5941 tgtctatatc ctagcctatc ctctcttctt tactgataat ccaatcgtca aaaaggtcta
     6001 ccttgttatg gggattcaac tcattgccca gattttttca atcgaatggg tcaatgaagc
     6061 tctggaaaat tacagttttc tcttttacaa aactgccttc atccgtatcc tgatgctggt
     6121 ctctattttc ttatttgtta aaaatgaaca cgatattgtt gtctatacac ttgtgatgag
     6181 tttatcgacg ctgattaact acctgattag ttatttttgg attaaaagag acatcaaact
     6241 tgttaagatt cacctaagtg attttaaacc actctttctc cctctgacag ccatgttagt
     6301 ctttgccaat gccaatatgc tcttcacttt tttagatcgc ctcttcctcg ttaaaacagg
     6361 gattgatgtc aacgttagtt actataccat agctcagcga attgtgaccg ttatagctgg
     6421 ggttgtaaca ggtgcaattg gagtgagtgt gcctcgtctc agttactatc tggggaaagg
     6481 agacaaagaa gcctatgttt ctctggttaa tagaggtagt cgaatcttta acttctttat
     6541 cattccactg agttttggac tcatggtttt aggaccaaat gccatcctac tttacggtag
     6601 tgaaaaatat atcggaggcg acatcttgac ctctctcttc gcttttcgta cgattatcct
     6661 ggccttagat accattcttg gttcccaaat tctctttacc aatggctatg aaaaacgtat
     6721 cacagtctat acagtctttg ctgggctact caatttgggc ttgaatagtc tcctcttttt
     6781 caaccatatc gtggctcccg aatactactt actgacaact atgttatcag aggcttctct
     6841 acttgttttc tatatcattt tcatccatag aaaacaactc atccacttgg gacatatctt
     6901 tagctatact gttcgatact ctctcttttc actttccttt gtagcaattt atttcctgat
     6961 taatttcgtg tatcctgtgg atatggtcat taatttgcca tttttgatta atactggttt
     7021 gattgtcttg ctatcagcta tctcttatat tagtctactt gtcttcacaa aagatagcat
     7081 tttctatgaa tttttaaacc atgtcctagc cttaaaaaat aaatttaaaa aatcttagga
     7141 gtttaaaatg aaacaactaa ccgttgaaga tgccaaacaa attgaattag aaattttgaa
     7201 ttatattgat actctctgta aaaagcacaa tatcaactat attattaact acggtactct
     7261 gattggggcg gttcgacatg agggctttat cccttgggac gacgatattg atttatccat
     7321 gcctagagaa gactaccaac gatttattaa catttttcaa aaggaaaaaa gcaagtataa
     7381 gctcctatcc ttagaaactg ataagaacta ctttaacaac tttatcaaga taaccgacag
     7441 tacgactaaa attattgata ctcgaaatac aaaaacctat gagtctggta tctttatcga
     7501 tattttccct atggatcgct ttgatgatcc taaggtcatt gatacttgtt ataaactaga
     7561 aagcttcaaa ctgctgtctt tcagtaaaca taaaaatatt gtctataagg atagcctttt
     7621 aaaagattgg atacgaacag ccttttggtt gctccttcga ctggtttctc ctcgttattt
     7681 tgcaaataaa atcgagaaag aaattcaaaa atatagtcgt gaaaatgggc agtatatggc
     7741 ttttattcct tcaaaattta aggaaaagga agtcttccca agtgctacct ttgataaaac
     7801 aatcgattta ccctttgaga atttaagcct tcctgcacct gaaaaatttg atactatttt
     7861 gacacaattt tatggagatt atatgaccct accaccagaa gaaaaacgct tctacagtca
     7921 tgaatttcac gcttataaat tggaggatta ggatgcaata tttagaaaaa aaagaaatta
     7981 aagaaattca actagccctg ctggactata ttgatgagac ttgtaagaaa catgatattc
     8041 cttattttct cagttatgga accatgcttg gagccatccg ccacaaaggt atgattcctt
     8101 gggatgatga tattgatatt tccctttatc gtgaggatta tgagcgttta ctgaagatta
     8161 ttgaagaaga aaatcaccct cgctacaagg ttctttccta cgatacatct tcttggtact
     8221 tccataattt cgcatcgatt ttggatactt ctactgttat agaagaccat gttaagtaca
     8281 agcgtcatga taccagcctt ttcatcgatg tcttcccaat tgatcaattt acagacttga
     8341 gcattgtcga caagagctat aagtatgtgg cccttcgtca actagcttac atcaaaaaat
     8401 cacgagcagt tcacggtgat agcaaactaa aagattttct tagattatgt agctggtacg
     8461 ctctccgatt tgtcaatcct cgctactttt acaagaaaat tgatcaacta gtcaaaaatg
     8521 ctgtaaccaa cattcctcaa tatgaaggag gagttgggat cggtaaggaa gggatgaaag
     8581 aaatcttccc agttgatacc tttaaagaac tgattttaac tgagtttgag ggccgtatgt
     8641 tgcctgttcc caaaaaatat gaccaatttt taacccagat gtatggcgat tatatgacac
     8701 caccatcaaa agaaatgcaa gagtggtata gtcatagcat taaagcttat cgcaaaaact
     8761 gattgagggg gattatacaa actactaaga tagaggttat tcaaaaacat aattttagta
     8821 gaaaatgaaa tacatattcc cacaataaaa cgcatcatat caaggttttt gaaaaacctt
     8881 gatatgatgc gttttataat tttaaagact ttttt
//
LOCUS       DAVBHS010000085         8887 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVBHS010000085 DAVBHS010000000
VERSION     DAVBHS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8887
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.085"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            170..856
                     /locus_tag="VQ651_001399"
     CDS             170..856
                     /locus_tag="VQ651_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2754380.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKAAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMAAISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPL
                     EIAKKFIEVV"
     gene            857..1789
                     /locus_tag="VQ651_001400"
     CDS             857..1789
                     /locus_tag="VQ651_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2754381.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSPFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFFFPAPL
                     IFALILNEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGIINSLLSSLGMEP
                     ISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTLYEAADMDGANRWHKMWH
                     VTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVSDVLSTYVYRQGILNSDF
                     SYSAAVGLFNNIVALLFVLLANKIAKKLGEEGIV"
     gene            1800..2729
                     /locus_tag="VQ651_001401"
     CDS             1800..2729
                     /locus_tag="VQ651_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2754382.1"
                     /translation="MIFSKNTKKSNRDKETFVDYLFIFILIIITLLMLYPLWWVVMTS
                     LSEPVWLSKNVVKLFPLKIDITAYKFIFGDSKLWQAYGNSVLYAALSTIVSVAVCSMY
                     AYPLTIPEIRGKKFWNTLILIPMFFSGGMIPLYIQVANLGLLNTAWALVLPGAVSTYY
                     IILFRTQFKSIPMELRESARIDGASEFRIFWNLVLPLSKVIFMIIALYCIVGSWNSFT
                     PPLLYLNDDTKMPLSIYLRNLVVTNTTDTAELSLSAGNTYYQQIAAQGGGIGMRIAIK
                     MGTIVVSVIPIMMIYPFIQKHFVKGILLGSVKG"
     gene            2743..4182
                     /locus_tag="VQ651_001402"
     CDS             2743..4182
                     /locus_tag="VQ651_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754383.1"
                     /translation="MQNLKKYLLLDSGLVVGYTLENAQLKLNKLKKDTENNPLFGEEY
                     FAENPKLWEVRFDNSYPNVIYDKELKLYRCWYQTFVSDEASEETPLAERGEKEYIVKS
                     SRMTALCYAESKDGVKWEKPNLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRY
                     KLVSKLEFSPNLHYMVVVFSEDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVIT
                     RTWANNVRLSAITFSDDFINWSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHE
                     GDTLNENYDKVDLELKFSANLEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPI
                     IEDDKIWFYYMGGNGNHTGFRESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYD
                     DEIYILADIPENGYIKVAVGTKYGNAYSDFGQEDISLVQVSDGKYKLEFNKKSILDLG
                     NSPVAFKFEIENAKLYGIEGSIQQHYLKY"
     gene            4251..5828
                     /locus_tag="VQ651_001403"
     CDS             4251..5828
                     /locus_tag="VQ651_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2754384.1"
                     /translation="MVKLNKVLKGSVAFLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDETLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSQHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKSFDWQPLVYPEINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEELDVLTKSEAGLNTFFDENIIKFIEGQRDMSEWDSFINEAKSKDLDK
                     VTKVYNDAYKRVKDRMK"
     gene            5888..7348
                     /locus_tag="VQ651_001404"
     CDS             5888..7348
                     /locus_tag="VQ651_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2754385.1"
                     /translation="MYDVVIIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIKKGGTVFNSMEELMNVNDFYQITIPYQPE
                     VYESVLTDMLIESKVDILLNVEVNNIIYDSKNIKSLEILSNQEVKLIDSKVFIDATGN
                     ATIASASGADVYEKNSSYGCLMRVGGVDISKTIEHIEKTKQWNYIEGYNQWLEEVLAN
                     RKYRATGAVLKDPVSYDHAPMLSKDDHFMNDEKWNYIKERWEKFRFIYTLEVSLFRNE
                     LKKAVESGDFNFEIYHDDKSGVTMNGDGISYGSWGQNVALINVAKAFGFNPKYMEDES
                     HANILSRKYNLTFLSFLKKYVPGFENSFILDQGTRATNRSCRHIDNSSMMDEILDFPM
                     YTFKTMYSYQTPKEITYKSIVGGKLNNLFVIGKGAYQSESFRSQISCMLMGISSAAAA
                     KTIIEDKSNTLNINRDLFKSNLDILFTWEFSNWITD"
     gene            7370..7825
                     /locus_tag="VQ651_001405"
     CDS             7370..7825
                     /locus_tag="VQ651_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET2754386.1"
                     /translation="MNDTNKNQYFVYEFDTSFVEMSYSQFFEGYCILFSKEPYPSLSD
                     MPMEFRKEFLMEMSILGDAMLEVLNAYRINYDILGNSTPVLHAHLFPRYMWEEEERRK
                     TVVHRYDSSVFSNPEFEFTIEKHGPIIKKLRSAIDRHYREYQQLLDDSN"
     gene            7912..8317
                     /gene="cdd"
                     /locus_tag="VQ651_001406"
                     /pseudo
     CDS             7912..8317
                     /gene="cdd"
                     /locus_tag="VQ651_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221146.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
     gene            8330..8779
                     /locus_tag="VQ651_001407"
     CDS             8330..8779
                     /locus_tag="VQ651_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2754387.1"
                     /translation="MELVEFKNGHVNTVHEHIAITKVKEFLNNHDILKDKGGIHKIST
                     DFFYNIIDMETTTEENRPWESHKEYYDVHYLLNGEETILYNFLSQMELSEYKVDDDWQ
                     QMNGTALFSIKLKKDMLLLLEPNDAHRTGLLVEEPLNIKKVVFKVKI"
BASE COUNT         3093 a         1153 c         1666 g         2975 t
ORIGIN      
        1 tagtgtattg aatctataac agtacgcctt aaatgctaaa atatttctat aaattaattt
       61 aactttcctg atagggctgt tcatatctta tttcaattca ctatataaat aaaattgtag
      121 tagatgttat tgaattagta aaaaaaataa aaaaacggag gttttctgaa tggaaactaa
      181 aaaaattaaa aatttaaaag gtcaaataat tgtttcctgc caagcgttag aaggagagcc
      241 actttatact ccaaatggag gagtgatgcc gttattagca aaagccgcct ttcaagcagg
      301 cgctaaaggt attcgtgcta attctgtccg cgatatttca gaaataaaag aagaagttga
      361 cttaccaatt ataggtatca taaaaagaga ttacgatggc tttgaacctt ttatttcagc
      421 aactatgaaa gagattgatg aattagttag tgaaggagta gacattttag ctttggattg
      481 tactaatcgt tcaagaccag gttatgataa tattactgac tttattcatg atattaaagt
      541 caagtatcca aaccaattat taatggctgc tatttctact tttgaagaag ggaaagtagc
      601 tgctgaatcg ggagtagatt ttgtgggtac gacattatct ggttatacac catatagccc
      661 taaaaaagat aatcctgatt ttgagttagt tgaaagattg gtaaaagagc ttgacgtacc
      721 agttattgct gaaggaagaa taagtactcc ggaacaagct agaaaaatgt tagatttagg
      781 tgcttacgca gtggttgttg gaggagctat cactcgtcct ttggaaatag cgaagaagtt
      841 tattgaggta gtttagatga atcaagaaat attaaaaaag agtaagattc aattgacagt
      901 tgaacatatt caaaaatatt ggattttata tttaatgatg attccagcaa ctgttctact
      961 aattttattt acgtatggac caatgtacgg cattattatg gcctttcaag actttactgt
     1021 cttcaaggga tatactggaa gtccatttgt cggtttaaaa cattttcaaa gattgttctc
     1081 agatcctttg ttttatagat tgttcaagaa tacgttcatg gtaggggttt tagatttttt
     1141 attctttttc ccagctccgc ttatttttgc actcatatta aacgaagtca gaaaagtaag
     1201 atttaagagt gtagttcaat ctattagtta tcttcctcat tttattcccc tagtggttat
     1261 ggttggtatt atttttgaac tatttggttc atatggtatt attaacagtt tactttcatc
     1321 acttggaatg gagccaataa gtttctttac aaaatctgaa tggttcttgc ctctctacat
     1381 tggttcagga gtatggaaaa caataggttg gggttctatc atttatatgg gagcattaac
     1441 caatatagat tcaactttat atgaagcagc agatatggac ggggctaata gatggcacaa
     1501 aatgtggcat gtaactctac caagtttaag acctacagta gttactttgt ttatactaaa
     1561 tgctggtggt atcatgcaag taggatttga aaaggtattc ttgatgtcta gtcctgcaac
     1621 atatgaagta tctgatgtat tatctactta tgtttataga caaggtattt taaattctga
     1681 tttcagttac tcagctgcag taggattatt taataacata gtagctttgt tatttgtttt
     1741 attagctaat aagattgcta aaaaacttgg cgaagaagga attgtataag gggggtaaaa
     1801 tgattttttc aaaaaataca aaaaagagta atagagacaa agaaaccttc gttgattatc
     1861 tctttatatt cattttaata ataataacct tacttatgtt gtatccatta tggtgggtag
     1921 tgatgacgtc actttcagaa ccagtttggc tatctaaaaa cgttgttaaa ttatttccct
     1981 taaaaataga tattactgca tataagttta tttttgggga ttctaaacta tggcaagcat
     2041 atggtaatag tgtattgtat gctgcactat ctacaattgt tagtgttgcg gtatgtagta
     2101 tgtatgccta tccacttaca attcctgaaa ttaggggtaa aaaattctgg aatactctta
     2161 ttttgattcc aatgttcttt agtggaggaa tgatcccgct ttatatacaa gttgcaaacc
     2221 tcggtttatt gaatactgct tgggctcttg ttttacctgg tgctgtttct acatattata
     2281 taattttgtt tagaacacag tttaaatcaa tcccaatgga gcttcgtgag tctgctagaa
     2341 ttgatggggc aagtgaattt agaatttttt ggaatttagt attaccatta tcaaaagtta
     2401 ttttcatgat tatagcactt tattgcatag ttggttcatg gaatagtttt actcctccat
     2461 tactttattt gaatgacgac actaagatgc ctttatctat ttatttgaga aatttagtcg
     2521 ttacaaatac aactgatact gctgaattat cactttctgc aggtaataca tattaccaac
     2581 aaattgctgc tcaaggtgga ggtattggta tgcgtattgc tattaagatg ggtactattg
     2641 ttgttagtgt tattccaatt atgatgattt atccatttat tcagaaacat tttgttaagg
     2701 gaatcctgct aggttctgtg aaaggttaat aaggtaatta ttttgcaaaa tttaaaaaaa
     2761 tatcttttat tagatagcgg tttagtagtt gggtatacat tagaaaatgc tcaattaaaa
     2821 ttaaataagc taaaaaaaga tactgaaaac aatccactat ttggtgaaga gtattttgcc
     2881 gaaaatccta aattatggga agttcgtttt gataattcat atccaaacgt tatctatgat
     2941 aaagaattga aattatatag atgttggtat caaacatttg ttagtgatga agcaagcgaa
     3001 gagactcctt tagcagaacg tggagagaag gaatacattg tcaagtcatc tagaatgaca
     3061 gcactttgct atgctgaaag taaagatgga gtaaaatggg aaaaaccaaa tctcaatcta
     3121 gtaaaattta aaggttcaaa agataataat attgttctga tgcatgcaca cggtgcaggt
     3181 atcatgctag atgaacagga gttggatcct aagaaacgat ataaattagt ttcaaaatta
     3241 gaattttctc cgaatcttca ttacatggtt gttgtttttt cagaagacgg tattcatttt
     3301 gacgaaccta ttccatggaa aaaatggaat cctgcagcag atagtcataa ttttccattt
     3361 agagatccaa aaactggtaa atacgctgtt attacaagaa cctgggcaaa taacgtacgt
     3421 ctaagtgcaa ttactttcag tgatgatttt attaattggt cagaacctaa agaaatcctt
     3481 aggggagatg gatttgaaga tcaaatttat tctatgccag taatttcata cgcggatatc
     3541 tatttaggtt ttgcttcaat gtaccatgaa ggcgacactt taaatgaaaa ctatgataaa
     3601 gtagacttag aattgaaatt ctctgctaat ttggaacaat gggatagtgt atgtaagggc
     3661 gactatctca ttgaaagggg aggtggagta tacccgcaga atgctgattt tgatgctagt
     3721 tgtattttca cggctgctcc aataattgaa gatgataaga tttggttcta ctatatgggt
     3781 ggaaatggta atcacactgg cttccgagaa agttcattag ctagaggata tattaagaaa
     3841 gaccaatttg cttattatac taataagaga gaaggaattc cagctaaaat atctacagtt
     3901 agcttccaaa cttacgatga tgaaatttat atcttagctg atattcctga aaatggttat
     3961 attaaagttg cggtaggtac taaatacggt aatgcttatt cagatttcgg tcaggaagat
     4021 atctctcttg ttcaagtttc cgatggtaag tataagttgg aatttaataa aaaatctatc
     4081 ttagatttag gtaattctcc agttgcgttc aaatttgaaa tcgagaatgc aaaattgtat
     4141 ggtattgaag gtagtattca gcaacactat ctcaaatatt aaccagaata attcgttaac
     4201 aaataatctc tatattagtg gagatagata tataaaatgg aggtcttatt atggtaaagc
     4261 ttaataaggt attaaaaggg agtgttgcat tcctgagtgc aagtttattg ctagcagctt
     4321 gtggcaataa cgctaattca aagcccgctg aaaaaacagc tgatggtaaa acgaaaattt
     4381 cgtttctatc tacatggcaa attaatgatg ttcgtccgcc taaagatgaa acacttgttg
     4441 gtcaatggtt agaagaacag ctaggtattg atattgatct tgtaactatt cctggcgagg
     4501 gagtaagttc taaagtaaat tctcttatta ctagtaacca gttaccgact gttttgttga
     4561 caacaggaga taagagtgat atcgctggta tcaacaaact tggtaaacag ggtgcattct
     4621 tagatttgtc acaacacatg gacaaactac caaactataa gaagtacctt gaaaaaaata
     4681 atgcattagc tcaaatagaa gatgatgaaa aacatattta tgctttcaca aaattcttta
     4741 ctgatgagaa tatcatgtat actacaccaa ttctgagaaa agacttactt gttggttcag
     4801 aatttgaaga tctttcaaaa attaaaactg ttgatgacta tacaaaagtt ttgaaatatt
     4861 tgactgaaaa acaaggttct ccagcattta ttcaacgtaa tggctatgaa ggcttcatga
     4921 agcgagttac accattgtgg aatttaagcc atcgtacata ctatgactat gaatcagatt
     4981 catataaaca tccagttgaa caacctcaat tgaaacaatt cgttgaatgg ttgaaagaat
     5041 tacgtaaaga taacgtactt caccctgact gggcagttat gaaagatgaa acttgggaag
     5101 gtctattggc ttctaacaag ggttcattca ctgttgaccg tatgaatatc attggagata
     5161 ataactttga taaatcattt gattggcagc cacttgttta tccagaaatt aatggaaaac
     5221 gttatttgca accgaaaaca ccgtacattt ctgattcagg ttgggtaatc aatgccaatg
     5281 caccaaaaga agaaattgat aaagcgttag aattctttaa tttcttatac gctgatgaaa
     5341 atcaagaaaa acttgctatt ggatttgaag gtaaaacatt tacacgtgaa aaccctaata
     5401 ctcaagcagg tattaaatgg ttgattcaaa tttacggtga aaatgctgga aatgataaag
     5461 cagaactgct gtttaaacat ggtaaccaag catttactcg cgttcaaact aaattagata
     5521 ttgaggcaca gccaggaaca ttccctaaag ttatgtatga tcaagtagat aaaattaaga
     5581 aagatttagg tggttttaga ccagatactc cggttctttc ctttacaact gaagaattag
     5641 atgttttaac taaatctgaa gcaggattaa atactttctt tgatgaaaat attattaaat
     5701 tcattgaagg acaaagagat atgagtgagt gggattcatt tatcaatgaa gctaaatcta
     5761 aagacttgga taaagttaca aaagtatata atgatgccta caaacgtgtc aaagatagaa
     5821 tgaaatagac taaatggtag ctctctggtt tttcagagag ctactactgc ctgaggggga
     5881 gtaagctttg tatgatgtag taattatagg tgcaggaagt tcgggcgcat tggctgcaat
     5941 tgcttcgggg cgtctagggt taaaaacttg tctaatagag aagggaagta gaattggtgg
     6001 agttcctatt aatacactaa tggggtcatt tgctaatcta tattttgatg attctggtag
     6061 agtaaatgca agtaaaattg ttggggaatt aatagaacgt attatcaaaa aaggtggtac
     6121 tgtctttaac tctatggaag aattgatgaa tgtaaatgat ttttatcaga ttacaattcc
     6181 ctatcaacca gaagtttatg aatcggtttt aactgatatg ttgattgagt ctaaagttga
     6241 tattttacta aatgttgaag ttaataatat tatttatgat agtaaaaata ttaaaagtct
     6301 tgaaatttta agtaatcagg aagttaaatt gattgattct aaagttttta ttgatgctac
     6361 agggaatgca acaattgctt ctgctagtgg agctgatgtt tatgaaaaaa attcttctta
     6421 cggttgttta atgagagttg gaggagttga tatctcaaag actatagaac atattgaaaa
     6481 aacaaagcag tggaactata tagaaggata taatcagtgg ttagaagaag tattagcaaa
     6541 tcgaaagtac agagccactg gagcagtttt aaaagatcca gtttcttatg atcatgcccc
     6601 tatgctttca aaagacgacc attttatgaa tgatgaaaaa tggaattaca ttaaagaaag
     6661 atgggaaaaa tttagattta tttacacttt agaagtatca ctatttagga atgaattaaa
     6721 aaaagctgta gaatctgggg attttaattt tgaaatttat catgatgata aatctggagt
     6781 cactatgaat ggcgacggta taagctatgg ttcatggggt caaaatgtag cattaattaa
     6841 tgttgcaaaa gcatttggat tcaatcccaa atatatggaa gatgaatcac atgctaatat
     6901 tctttctaga aaatataatt taacattttt atcttttctt aaaaaatatg taccaggttt
     6961 tgaaaattct tttatccttg atcagggaac tagagctact aacagatcat gtaggcacat
     7021 tgataattca tctatgatgg acgaaattct tgattttcca atgtatacat ttaagactat
     7081 gtatagctat caaactccta aagaaataac gtacaaatca attgtaggtg gaaaattgaa
     7141 taatttgttt gtaataggta aaggtgctta ccaatccgaa agttttagat cacaaattag
     7201 ttgtatgtta atggggatat catcagcagc agcggcaaaa actattattg aagataaaag
     7261 caatactcta aacattaata gagatttgtt taaatcgaac ttagatatat tatttacatg
     7321 ggagttttct aattggatta ctgattaaca aataataagg gaatattaaa tgaatgatac
     7381 taataaaaat caatactttg tttatgaatt tgatactagc tttgtagaaa tgtcttattc
     7441 tcagtttttt gaaggatatt gtattctttt ttcaaaagaa ccctatcctt ctttatctga
     7501 tatgccaatg gaatttagaa aagaattttt aatggaaatg agtatattag gtgatgcgat
     7561 gcttgaagtg ttaaatgcct atagaattaa ttatgatatc ttaggaaatt caacacctgt
     7621 acttcatgct catttatttc cgagatacat gtgggaagag gaagagagaa gaaaaactgt
     7681 agttcataga tatgattctt cagtttttag caatcctgag tttgaattta ctatagaaaa
     7741 gcatggacca attattaaaa aactgaggtc tgctattgat agacattata gagagtatca
     7801 acaattatta gatgattcaa actgaactcc acattttcga ttagatttaa taccttgtat
     7861 attacaacta ggttgtggat gatataattt tttgaaaaat atattttagg aatgacttat
     7921 aaagcaatta tatcagaggc tattaaatca atggccaaag cttatgctcc ttattctgat
     7981 tttaaggttg gagctgcgtt agtaactgaa gataaaaaat tttttctgga tgtaatatag
     8041 aaaatgctgc tttttctcct acttgctgtg cagaacgagt atctttttat aaagctgtat
     8101 ttaaaggaga acgttcgttt tcaagaacat gtattgtagg tggaaaaaat ggtatcttat
     8161 catcttttat agcaccttgc ggagtgtgtc gccaggtaat gatagaattt gtaatcctga
     8221 ttcatttgaa gttatattgg ctagtgatgt tgatacttat aaagtatata aacttaaaga
     8281 actactacca gttagtttta atcaagaaaa tttataacag gagggaacta tggaattagt
     8341 tgaatttaaa aatggacatg ttaatacggt tcatgagcat atcgcaataa cgaaagtaaa
     8401 agagtttcta aataatcatg atatattaaa agataaaggt ggcatacata aaatttctac
     8461 tgatttcttt tacaatataa ttgatatgga aactacaact gaagagaata gaccgtggga
     8521 aagtcataag gaatattatg atgtccatta tctgttaaat ggagaagaaa caattttata
     8581 taacttcttg tctcaaatgg aattgtctga atataaagta gatgatgatt ggcaacaaat
     8641 gaatgggaca gctttattta gtattaaatt aaagaaagat atgttacttt tgttggaacc
     8701 aaacgatgca catagaacag gcctattggt tgaagaacca cttaatatta agaaagttgt
     8761 ttttaaggta aaaatttaac tgaaagtact ttcggggatt aagaatttta aaatatttaa
     8821 gaaattgtat tctaaagttc taaaattata gatatttaat ttaaagcttg ttaaaataga
     8881 atagtaa
//
LOCUS       DAVBHS010000086         8867 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVBHS010000086 DAVBHS010000000
VERSION     DAVBHS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8867
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.086"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            120..965
                     /locus_tag="VQ651_001408"
     CDS             120..965
                     /locus_tag="VQ651_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2754388.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLTLDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            955..2058
                     /locus_tag="VQ651_001409"
     CDS             955..2058
                     /locus_tag="VQ651_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET2754389.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDLEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQIIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            2060..3046
                     /locus_tag="VQ651_001410"
     CDS             2060..3046
                     /locus_tag="VQ651_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2754390.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQIYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            3062..4495
                     /locus_tag="VQ651_001411"
     CDS             3062..4495
                     /locus_tag="VQ651_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HET2754391.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFIAKMLTKEN"
     gene            4524..5558
                     /locus_tag="VQ651_001412"
     CDS             4524..5558
                     /locus_tag="VQ651_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET2754392.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            5758..6495
                     /gene="mecA"
                     /locus_tag="VQ651_001413"
     CDS             5758..6495
                     /gene="mecA"
                     /locus_tag="VQ651_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HET2754393.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            6646..7932
                     /locus_tag="VQ651_001414"
     CDS             6646..7932
                     /locus_tag="VQ651_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET2754394.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            7934..8803
                     /locus_tag="VQ651_001415"
     CDS             7934..8803
                     /locus_tag="VQ651_001415"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET2754395.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTKGVRVEAK"
BASE COUNT         2718 a         1520 c         1884 g         2745 t
ORIGIN      
        1 acagaaaaat caaagtttga tagctatttg gatagcagtc aggctttgat tttttacagt
       61 ctgcaataga ttttcacccc ttgtttgtgg tataatgaaa cgatacgata gaaagaataa
      121 tgaacaatat gactgattta aaagcaattc aggctcgtag tctggagatg gctgaatatt
      181 ttgtggcctt ttgtaaagaa catgatttgc tctgttatct ctgtggcgga ggcgctattg
      241 gtgcccttcg aaacaaggga tttattcctt gggacgacga cctagacttt tttatgcctc
      301 gtaaagatta tgagaaatta gcagaattat ggcctcgtta tgcagatgaa cgttatttct
      361 tgtcaaagag tcacaaggat tttgttgatc gtaatctttt tattaccatt cgtgacaagg
      421 aaaccacctg tatcaagcct tatcagcagg atttagattt gccacatggt ctgaccttgg
      481 atgttttgcc tttggattat tatccgaaaa atccagctga gcggaaaaaa caggttcgtt
      541 gggccttgat ttattcactc ttttgtgcgc aaactattcc agaaaagcat ggtgctctca
      601 tgaaatgggg aagtcgcatt ttactgggtt tgactccaaa atctctccgt tatcgcatct
      661 ggaaaaaagc tgagaaagaa atgactaagt atgatttggc tgattgtgat ggcattacag
      721 aattatgctc aggtcctggc tacatgagaa acaagtaccc aatcacatct tttgaagaca
      781 atcttttctt gccatttgaa ggaacagaga tgcctattcc aatcggctat gatgtctatc
      841 tcagaactgc ttttggggat tatatgacgc ctccaccagc agacaagcag gtaccgcatc
      901 atgatgctgt catcgctgat atggataagt cttatacaga atacaaggga gaatatggtg
      961 gctaagaaaa aaatcttatt ttttatgtgg tctttttctc ttggaggtgg tgcagagaag
     1021 attctatcaa ccattgtttc aaatctggat ctagaaaagt atgatattga tattcttgaa
     1081 atggagcact ttgacaaggg atatgaatct gttccaaagc atgtacgcat tttaaaatcc
     1141 cttcaagatt atcgccaaac cagatggtta cgagcttttt tgtggagaat gagaatttat
     1201 tttccaagac tgactcgtcg tttgcttgta aaagatgatt atgatgttga agtttctttt
     1261 accattatga atccaccact gttgttctct aaaagaagag aagtcaagaa gatatcttgg
     1321 attcatggaa gtattgaaga acttcttaag gatagctcta aaagagaatc acatagaagc
     1381 cagttggatg ctgcgaatac aattgtaggg atttcaaaaa agaccagcaa ttctatcaag
     1441 gaagtttatc cagattatgc ttctaaatta cagataatct acaatggata tgattttcag
     1501 actattctag aaaaatctca agagaagatc gatatcgaga ttgctcctca aagtatctgt
     1561 actatcggac ggattgagga aaataagggt tctgaccgtg tagtggaagt gatacgatta
     1621 ttacaccaag agggaaaaaa ctatcatctc tattttatcg gggctggtga tatggaagag
     1681 gaactgaaaa aacgagtcaa agagtatggg attgaggact atgtacattt ccttggttat
     1741 caaaaaaatc cttatcagta tttatctcag acgaaagttc tcttgtctat gtctaaacaa
     1801 gaaggttttc ctggagtgta tgtggaggcc ttgagtctgg gactcccttt tatctctacg
     1861 gacgttggag gggctgagga attatcccaa gaaggacgat ttggacaaat cattgagagc
     1921 aatcaagagg cagctcaggc gattactaat tacatgactt ctgcctcaaa ctttgatgtc
     1981 gatgaggcta gccaattcat tcaacaattt acaattacaa aacaaatcga acaagtagaa
     2041 aaactattag aggagtagca tggaaactgc attaattagt gtgattgtgc cagtctataa
     2101 tgtggcgcag tacctagaaa aatcgatagc ttccattcag aagcagatct atcaaaatct
     2161 ggaaattatt cttgttgatg atggtgcaac agatgaaagt ggtcgcttgt gtgactcaat
     2221 cgctgaacaa gatgacaggg tgtcagtgct tcataaaaag aacgaaggat tgtcgcaagc
     2281 acgaaatgat gggatgaagc aggctcacgg ggattatctg atttttattg actcagatga
     2341 ttatatccat ccagaaatga ttcagagctt atatgagcaa ttagttcaag aagatgcgga
     2401 tgtttcaagc tgtggtgtca tgaatgtcta tgctaatgat gaaagcccac agtcagccaa
     2461 tcaggatgac tattttgtct gtgattctca aacatttcta aaggaatacc tcataggtga
     2521 aaaaatacct gggacgattt gcaataagct aatcaagaga cagattgcaa ctgccctatc
     2581 ctttcctaag gggttgattt acgaagatgc ctattaccat tttgatttaa tcaagttggc
     2641 caagaagtat gtggttaata ctaaacccta ttattactat ttccatagag gggatagtat
     2701 tacgaccaaa ccctatgcag agaaggattt agcctatatt gatatctacc aaaagtttta
     2761 taatgaagtt gtgaaaaact atcctgactt gaaagaggtc gcttttttca gattggccta
     2821 tgcccacttc tttattctgg ataagatgtt gctagatgat cagtataaac agtttgaagc
     2881 ctattctcag attcatcgtt ttttaaaagg ccatgccttt gctatttcta ggaatccaat
     2941 tttccgtaag gggagaagaa ttagtgcttt ggccctattc ataaatattt ccttatatcg
     3001 attcttatta ctgaaaaata ttgaaaaatc taaaaaatta cattagaacg ggggtgacca
     3061 agtgattgat gggaaacgat tattatttag tttgaccata gtcagttatg ccttgacgct
     3121 agtaagtgga attgtgtatc tgtttaataa taataatgtt agcttacttt ctactttatt
     3181 gttcttgttg gttagtagct taattgcttg ttggaatgat atcaagtatt acttaatcca
     3241 ttttattttc tatttaacca tttttgtatt tctggtatca agaccgacca ttgattattt
     3301 tagggatggt gctttggata cctatcatcc aatagcctat cgttttgcct ttatagttgt
     3361 catggtttcg attctgggct tgaccacagg aggcattctg gctcgttact tcatagctag
     3421 gaagaaaata aaagtagcaa atataggaaa ttctctaaaa gaggtttata tcaagcggtt
     3481 acgctttgta tcactaggag tttttcttct aacttatcct ttctatttca ttcggttatt
     3541 tgaacggctc ttgtatcgtt tgcagacttc ctactatgcc tactatgcaa attttgaaag
     3601 taaactgcct tattttacct acattttgtc tacctttacg gtctatgcaa tgtgtatgta
     3661 tctggcaacc aagccaaaga aattgcaggc cacagcagtg cttgtctcct ttattgcagc
     3721 taatactatt catttggcaa ttgggacacg aaatcccttt attttaagta ttttatttgc
     3781 ttttgtttat tactttatgc gggagcaaac tgaaaaagga aaatggattg ggtttaaaga
     3841 aaagttagcg atttttgtag gttctcctat tctcatgtta gcgatgggag tactcaatta
     3901 tgtacgggat aatgtccaag tttcccatac aggtttctgg gatatcttac ttgactttat
     3961 ctataaacaa gggactagtt ttggtgttct ggctcgaggt tttctattta acagtagcct
     4021 cccttaccga gatttccgta attttacttt tggtcctgtt cttgattatt ttgcaagggg
     4081 gagtttggga gccattttcg gaggaaaagc ctttgaacat acaaccaata gtgtggaact
     4141 agctattgat agtaatagtt atgcccacaa tctatcctat cttgtcttga acaaggaata
     4201 cctgaaaggg catggtatcg gaagtagtta tatcatggag ttgtataccg actatggtat
     4261 gattggagtc tttctgctta gtttcttact cggcgtatta tttatagcca tgctgcaagt
     4321 agcctatcgc tcaaggacaa tcctatttgc tttatcccta ctcatcttga ataatctatt
     4381 ctttatgcca agaagcagct tttcagaaag tttcttcaat ttatttacaa tgcaattctg
     4441 gggaattgtt cttgtgatta tatttatagc aaaaatgctt acaaaggaaa actagtatct
     4501 actaaacaaa ggagaaaaaa atcatgtttg aacattattc agtagctgat ttgtttgcaa
     4561 atctttataa aaagcgaaaa gcaaatattt tagctctcat cgctttattt gctctcattg
     4621 ctgtaccatt tacaattaaa gcagttagga ataaaaacac tgtcaaagat acaacaagtt
     4681 attcaactta tcttatctat aaaatcactc ctccaaaaga gtcggccaaa acgattttga
     4741 atcatcaaat tggtggttat agtgattttt atgggaaatt gattgatggt aatttgaatg
     4801 gagcttatct tttcaatgat gtagaaccca gtgagttgaa aaaaattgcc agtgaattag
     4861 acacgacaga aacaaccttg aaaaattcaa cgaatgacta ttggtggaaa aaattgaccg
     4921 tctactatat gattgacgat gcaggggttg gtgtgaaaat tttgacatca agtaaagatg
     4981 ccaatgactt gttagagaaa aaaattgatg ggttgattga gaaatttaaa catgcctatg
     5041 caaatgtgaa aattgaaaaa ctggaaacca tcaactctaa agaattgaac gcaaatggtg
     5101 aaacagcgct tggcttaaat gtgaaaaatc tgattcttcg tttagttgtt attggagtgg
     5161 tttgtgtgat tttggttgtg atgggaaatg tgttagttta tctctttaat ccaacaatca
     5221 atagagtagg tgatttttct cagtatcaaa ttgattttgt aacagagatt acaacaattg
     5281 ctaacctagc agatgttttg tcatacaaaa atactggaca ggaattgacc atcgttagct
     5341 caaataaagc tatcctagat aaattgaaac agagtcaaga agctttaaaa ggaatgcatt
     5401 ttgtagattt acaggatgta tcatctcttt tggaaagaga tacagtcctt cttgttgaag
     5461 agtacggagt gactcgttat aagaaatttg agcaaagtct tcaaattctc agaaacttaa
     5521 atcgttctat cctcggtgta gcaaccttta aattataagt attctgatgt ataaggtctt
     5581 caaaatcggc tcttagccga ttttgaaggc tttttctatc tgtctgcaga aattttctct
     5641 tggagtctcc tatagaaaga ccctagggaa aatctatccg tatagaggag ccttcttttg
     5701 ataaaatcgt aaaaatctag tataatagat agaactgaaa gtatgaggtt actagcaatg
     5761 aaaatgaaac aaattagtga tacaacttta aaaatcacga tgtctttaga ggatttgatg
     5821 gatcgtggaa tggagattgc tgactttctc gttcctcaag aaaaaacaga agagttcttt
     5881 tatgctatct tggatgagct agagatgcct gatagctttc tggatacagg catgttgagc
     5941 ttccgtgtga ctccaaaacc tgataaggta gatgtctttg tgaccaagtc aaagattgac
     6001 caaaatctag attttgaaga cttatcggat ttgccagata tggaagaatt ggctcaaatg
     6061 tctccagatg aatttatcaa aaccctggaa aaaagcatcg cagacaaaac caaggatgat
     6121 atcgaagcca ttcaatctct tgagcaagtt gaagccaagg aagaagagca agagcaggct
     6181 gaacaagaag ctgagagtaa gaaagaacct tacatctact acatcctttc ttttgctaag
     6241 ttggctgact tggtagcttt tgccaagaca gtgacttttg aaatggaaac ttctgaactc
     6301 tacaaaatga acgagcgcta ttatttgacc attttagtgg atattgaaaa tcatccaagc
     6361 ccatatccag cttggctgtt ggcccgtatg cgcgagtttg cagacgatag tgatatcagt
     6421 cgctcagtct tacaagagta tggtcaagtc ttgatgagtc acgatgcagt gctcaatctg
     6481 caaaaaatcg gctaatcttt tctggaaagc tattttcaga ttttaagaag agcgaatcgt
     6541 ctgattcgtt ttttcttttt atactgaaat agtgatttac tataatagga attttcacaa
     6601 aattctgtta taatggctat attagaaaat ttcgaggaga taaagatgac agttaaaatt
     6661 gctttactag gatttggtac cgttgcaagt ggtgtgcctt tcctcctaaa ggaaaatgga
     6721 ggaaaaatca atcaatcagc acattcagat atcaaagttg ctaaggtatt ggtcaaggat
     6781 gaagatgaaa aaaatcgctt gcttgcagca gggaatgact ttaactttgt aaccaatgtg
     6841 gatgatattt tatcagacca ggatattact atcgtagtgg aattgatggg gcgtattgag
     6901 cctgctaaaa cctttatcac tcgtgccttg gaagctggaa aacacgttgt tactgctaac
     6961 aaggaccttt tagctgtcca tggcgcagaa ttgctagaaa tcgctcaagc taacaaggta
     7021 gcactttact acgaagcagc agttgctggt gggattccaa ttcttcgtac tttagcaaat
     7081 tccttggctt ctgataaaat tacgcgcgtg cttggagtag tcaacggaac ttccaacttc
     7141 atggtgacca agatggtgga agaaggctgg tcttacgatg atgctcttgc ggaagcacaa
     7201 cgtctaggat ttgcagaaag cgatccgacg aatgacgtag atgggattga tgcagcctac
     7261 aagatggtta ttttgagcca atttgccttt ggcatgaaga ttgcctttga tgatgtagcc
     7321 cacaagggaa tccgcaatat cacaccagaa gacgtagctg tagctcaaga gcttggttac
     7381 gtagtgaaat tggttggttc tattgaggaa acttcttcag gtattgctgc agaagtgact
     7441 ccaaccttcc tacctaaagc gcacccactt gctagtgtga atggcgtaat gaacgctgtc
     7501 tttgtagaat ctatcggtat tggtgagtct atgtactacg gaccaggtgc gggtcaaaaa
     7561 ccaactgcaa caagtgttgt agctgatatt gtccgtatcg ttcgtcgttt gaatgatggt
     7621 actattggca aagacttcaa cgaatatagc cgtgacttgg tcttggcaaa tcctgaagat
     7681 gtcaaagcaa actactattt ctcaatcttg gctctagact caaaaggtca ggtcttgaag
     7741 ttggctgaaa tcttcaatgc tcaagatatt tcctttaagc aaatccttca agatggcaaa
     7801 gagggtgaca aggcgcgtgt cgttatcatc acacacaaga ttaataaagc ccagcttgaa
     7861 aatgtctcag ctgaattgaa gaaggtttca gaattcgacc tcttgaatac cttcaaggtg
     7921 ctaggagaat aagatgaaga ttattgtacc tgcaaccagt gccaatatcg ggccaggttt
     7981 tgactcggtc ggtgtagctg taaccaagta tcttcaaatt gaggtctgcg aagaacgaga
     8041 tgagtggctg attgaacacc agattggcaa atggattcca catgacgagc gtaatctctt
     8101 gcttaaaatc gctttgcaaa ttgtaccaga cttgcaacca agacgcttga aaatgaccag
     8161 tgatgtccct ttggcgcgcg gtttgggttc ttccagctcg gttatcgttg ctgggattga
     8221 actagccaac caactgggtc aactcaactt atcagaccat gaaaaattgc agttagcgac
     8281 caagattgaa gggcatcctg acaatgtggc tccagccatt tatggtaatc tcgttattgc
     8341 aagttctgtt gaagggcaag tctctgctat cgtagcagac tttccagagt gtgattttct
     8401 agcttacatt ccaaactatg aattacgtac tcgcgacagc cgtagtgtct tgcctaaaaa
     8461 attgtcttat aaggaagctg ttgctgcaag ttctatcgcc aatgtagcgg ttgctgcctt
     8521 gttggcagga gacatggtga ccgctgggca agcaatcgag ggagacctct tccatgagcg
     8581 ctatcgtcag gacttggtaa gagaatttgc gatgattaag caagtgacca aagaaaatgg
     8641 ggcctatgca acctaccttt ctggtgctgg gccgacagtt atggttctgg cttctcatga
     8701 caagatgcca acaattaagg cagaattgga aaagcaacct ttcaaaggaa aactgcatga
     8761 cttgagagtt gataccaaag gtgtccgtgt agaagcaaaa taaagaatag aagataggat
     8821 ggggaaactc ttgaccagag ggtttcatat cctttttgtg aaaagaa
//
LOCUS       DAVBHS010000087         8807 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVBHS010000087 DAVBHS010000000
VERSION     DAVBHS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8807
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.087"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(174..803)
                     /locus_tag="VQ651_001416"
     CDS             complement(174..803)
                     /locus_tag="VQ651_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET2754396.1"
                     /translation="MVLSKYYGVADGMNVEGRGSANFIKDNVLITAAHNYYRHDYGKE
                     ADDIYVLPTVSPSQEPFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLG
                     TLGLPTSQKNLTGITVTITGYPSYNFKVHQMYTDKKQVLSDDGMFLDYQVDTLEGSSG
                     STVYDASHRVVGVHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGW"
     gene            complement(848..1087)
                     /locus_tag="VQ651_001417"
     CDS             complement(848..1087)
                     /locus_tag="VQ651_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754397.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(1237..1503)
                     /locus_tag="VQ651_001418"
                     /pseudo
     CDS             complement(1237..1503)
                     /locus_tag="VQ651_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(1528..1836)
                     /locus_tag="VQ651_001419"
     CDS             complement(1528..1836)
                     /locus_tag="VQ651_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET2754398.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLRLQIAS
                     "
     gene            complement(1761..2003)
                     /locus_tag="VQ651_001420"
     CDS             complement(1761..2003)
                     /locus_tag="VQ651_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET2754399.1"
                     /translation="MSLADLLEELEAAKDSKKARPMEAYMRHQFSFLGIAVPERNKLY
                     KNIYQKRKKQRLSIGILQTLAGKRSLENTNMWLLTI"
     gene            complement(2004..2636)
                     /locus_tag="VQ651_001421"
     CDS             complement(2004..2636)
                     /locus_tag="VQ651_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2754400.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(2650..3645)
                     /locus_tag="VQ651_001422"
     CDS             complement(2650..3645)
                     /locus_tag="VQ651_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2754401.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLYDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKS
                     LSGKLNLLTEALQKAENHSLDQEEEIIEKERKRIARDLHDTVSQELFAAHIILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFRLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(3642..4340)
                     /locus_tag="VQ651_001423"
     CDS             complement(3642..4340)
                     /locus_tag="VQ651_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HET2754402.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(4418..5428)
                     /locus_tag="VQ651_001424"
     CDS             complement(4418..5428)
                     /locus_tag="VQ651_001424"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HET2754403.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(5412..6419)
                     /locus_tag="VQ651_001425"
     CDS             complement(5412..6419)
                     /locus_tag="VQ651_001425"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HET2754404.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLTPNPDYSLIQETIALMGDFLAVRGQNLRSFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(6406..7359)
                     /gene="mvaD"
                     /locus_tag="VQ651_001426"
     CDS             complement(6406..7359)
                     /gene="mvaD"
                     /locus_tag="VQ651_001426"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET2754405.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVIADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YSVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(7341..8219)
                     /gene="mvk"
                     /locus_tag="VQ651_001427"
     CDS             complement(7341..8219)
                     /gene="mvk"
                     /locus_tag="VQ651_001427"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HET2754406.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(8341..8670)
                     /locus_tag="VQ651_001428"
     CDS             complement(8341..8670)
                     /locus_tag="VQ651_001428"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2754407.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMIPHDFPLYLTVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
BASE COUNT         2572 a         2072 c         1564 g         2599 t
ORIGIN      
        1 tctactatac aagacgaaat tgaagaaatc tttccattat aaaacgcata ttatcaaggt
       61 ttttaaacac ctaataatat gcgttttttc ctattttaaa gacttttatc cacctattat
      121 acaaagttaa gtatccatca cagtttacaa ttagccttca ttacctaacc cattcaccat
      181 ccttcaagag agtaaccttt aagaaccgaa taaataaatg gcaaatttcg ttcatttaat
      241 ttaactgcac tgttaatttg attagctcca tctcctaaag tatgcactcc tactacacgg
      301 tgactagcat cataaactgt agatccacta gacccctcta aagtatcaac ttggtaatcc
      361 aagaacatgc catcatcact taaaacttgt tttttatctg tatacatttg atgaacttta
      421 aaattatatg atggatagcc tgtgatagtc acagttattc ctgtcaaatt tttttgacta
      481 gtaggaagac ccaaagtccc taattttgca ccaatgggct cttctagaat taataaagcc
      541 aagtcatatt cccttgcatc cttagaattt aaatttctaa attccttcaa ataacgaact
      601 tcctttactt tgatctttcc aaatggttct tgacttggac taacagtcgg aagaacataa
      661 atatcatccg cttctttccc atagtcatgt ctgtagtagt tgtgagccgc tgtaattaac
      721 acattatctt taataaaatt cgcacttccc ctcccttcta cattcattcc atcggctact
      781 ccataatact tagataaaac catagctgaa gtcgaatacg gatactgtaa agtattatca
      841 attttaatca aatcatcatt accgataata cttctgattg cttttggtag tatgaaccat
      901 atgttggtga aatctcagat aatgaagaat cattagatcc tggacctttt tctagtgtct
      961 cacttacctc atattcttca cccgtactag aaataacact caaagcagat actgtcgata
     1021 actggctagc caataaagta ctcgcaataa ttgaaatacc caatttttta taaacagttt
     1081 tcttcattat tgtatcctcc taatgtaatt atagcgtact attctaaatt tcttaatcta
     1141 ctatagaatc aagaaatcta ccaccttctt taaataccct ccattatcac ataaacaggt
     1201 aaacttttca attaatgact gcgcttttca atcacgctag aggtacttac ttgcttcttt
     1261 gatactaagt tcagccattc tttccttgtt tttctcaata aagcatgtta cccaagtggg
     1321 attcgttttg gagtagtctc gcagagtcca gccaatggct ttattgataa aaaattctgt
     1381 ttggttcaag ttatgaagta gaatcttttc cattaattga gtattggtct tctcttttct
     1441 taacaactgg tggtcaatag cgacacgtct cagccagata ttatctgata ggctccattt
     1501 tatactcaat gaaaatcaaa gagcaaacta ggaagctatc tgcaacctca aaacactgtt
     1561 ttgaggttgc agatagagct gacgtggttt gaagagattt tcgaagagta ttaagattat
     1621 ttcttctagt tcagggtgtt catacaccaa actccctact actcgatcta ggatatctac
     1681 cgtgtcccac caggattttg tcacgactaa ctgctctagc ttaggcaaat cggtttcctt
     1741 tagataagac tgcattgctt tcaaatagtt agcagccaca tattggtatt ttctaggctc
     1801 cttttcccag caagtgtctg caaaatccca atcgataatc tttgtttttt tcgcttctgg
     1861 taaatatttt tatagagttt atttctttca ggcaccgcaa tacctagaaa agaaaattga
     1921 tggcgcatat aggcttccat gggccttgct tttttagagt cttttgctgc ttctagctcc
     1981 tcaagtaaat ctgctaaact catctaaaac tcctcttgcc ccaccaaatg gtgctgaaag
     2041 gcatagacag ccgcctgggt acgatcgctg acttcaagtt tggcaagaat attggacacg
     2101 tgggtcttga ccgtcttgag agagataaag aggtcatctg cgatgcgctg attttcgtag
     2161 cccttggcga tgagttggag aacatctcgc tcacgcgcag tcaattcttc atgaagttcc
     2221 atatgattgc ggtggtattc aaccttcttg ctaacctctt gctcaatggc cagctcgcca
     2281 gcagctacct tactgacggc atgaagcaat tcatctgcac tagaagtctt gagcatatag
     2341 cctttggcac cagcatctaa gactggcatg attttttcat tgtccaaata agaggtcaca
     2401 atcaaaatct tggcttcagg ccattcttta aggattgcta aggtcgcgtc aatcccattc
     2461 atctcaggca tgacaatatc catgacaatg acatctggac gcagttccaa ggccaagtca
     2521 atcccttgag acccgttgga cgcctcaccc acaacttcta catcgtcttg gaggtcaaag
     2581 tagcttttca agcccaaacg gaccatttca tggtcatcta ctagtaaaat tttcatcttt
     2641 actcctttat cattccttat ctaacagggg aatacggata tcaactgcca gcccttgctt
     2701 gggagctgtt aataactgaa ccgtccctgc catatcttca acccgctcct tgatatttcg
     2761 cagtccataa ctcaagtcgt ctaagcttcc taaccggaaa ccaatcccat tgtccaccac
     2821 cttcagttgc aattcaacat ctgtctgata gaggtagaca tctaggcaag atgcctgggc
     2881 atggcggagg gtattgctaa tcaactcttg caggatacgg aagatatgct cctcgatttt
     2941 cttaggcaat ttcgtcatat tctgcttgag actaacccta agatcactct tgtcctcaag
     3001 ctcttttaag agaatctgaa tcccttctat caagctcttc tgctccagtt caactggtcg
     3061 caaatgcaag agcaaaaccc gcaaatcctt ctgggcagtt tctaaaatag ctgtgacact
     3121 ctgcaactgg gtctgcatct tttctctatc caatttcaaa gcctgctgac tgatacccga
     3181 taaaataatg tgggccgcaa acaactcctg actgactgta tcgtgcaaat cccgagcaat
     3241 tcgcttccgt tccttctcga taatttcctc ttcctgatca aggctatgat tttcagcttt
     3301 ttgaagagcc tctgtcaaaa ggttaagttt acctgataag gacttgaaac tggcatccaa
     3361 atctggatct acaacctgaa ctacttcttg ccctgccaat aaacgcttga gattagcctg
     3421 catttttctt agagaaagct cttcgatccc tcgccaaaac agggctaaga gacaggtcat
     3481 ggacatgctg aaaaccaaca ataaaaagac aaatttttct gttttttcga catcgtacaa
     3541 aaagatagac cagtcaaaat caagtatttc cagcaagctg tgggagaaaa aaaagacaaa
     3601 taggaaggag gtgagagcaa taatgacata ggcttgtttt ttcatcctct aaccacctcc
     3661 acatcaccaa tcatagtggt caagaaaatc ttgacactct tgttactctt gagatagtct
     3721 tttgtttctt gatgatagtg ttcattgcgg agggctcgct tgggctggtt gaaaaaagtc
     3781 aaatccccat agagacagtt aacgctgaga ctgacttcca catctacagg tacgatgatt
     3841 ttggtcgttc ctaccatctt tctgaggata atgacattgt catgattggt taagatgact
     3901 ctctccagat gaatagtgtc cttgcccatg aagcgaaaga gattgatatc atcaaattgg
     3961 caagtctggt agcttgaaaa atgatgaaga tttccaaacc aacgattttt ctccttctta
     4021 accgtcacaa cctcttcaaa aaccaaattg gtctgctctt tttcctggtt catcatcggg
     4081 taaagaagaa agagactata gataaccgca acaaaaatag ctagaatcac aaaaggattg
     4141 agcataacga tgaaaaagaa gagaatggtt gccgctacta aaagaagatt attcccctct
     4201 ttaccagtat agtagcgaat caaaagcaaa aagaggaata gtatcagcag aaaacgcgaa
     4261 aaatgctctg ataccatcaa aatcagagct cctgtcagaa gacaggcttc gataaataaa
     4321 aagattttaa attttctcat aggttcatcc tctcccttct attttatcac aattcaaaaa
     4381 agtcacctca gtctgaggat ggaaaaaaag gcgctggtta cgcctttttc atctgatcct
     4441 ttgcttcttt taattttcca taaagaagat agtctacttt ttgtagatct gctatggtgg
     4501 cacagttaag ggaacacata atcaagcgta gatctgcttt ccagccttgg acaatgccaa
     4561 tcacttcttc aactgtgtag gtttcaacca attccagaac ggttcgtgac aatcccacag
     4621 ccttagcacc aaaaaccaag cacttaatca tatccagcgg attccgaacc cctccactaa
     4681 ccaagagttc gaccttatct ttccattctt gggcattgag aagggcctgc atggtagact
     4741 gaccccattg attgaggtaa tcacgctggc cactacgacg gttttcgata taggcaaagc
     4801 tggtgccacc acgacccgat aggtccactg tacgaacacc gaattcatag gctctttcga
     4861 ttgtcttggc atccattcca aagcccactt ccttgaggac aataggaacg ggaatttgct
     4921 tgctataatc tgctagatgc gattgccagc ttctaaactt cctttctccc tcgggcatga
     4981 gtaattcctg catgacattg acatgcactt gcaatagaac aggattcatc tcttctacag
     5041 tctgaagtcc taactcgaca ggtttgtcca atccaatatt ggttccaagg aggagattgg
     5101 gatgactaga cttgacagaa aaagaatcat ccgttggatt tttgagggct gcgctataag
     5161 aacccgttac aaataaaata ccacaggatt ccgccacctg agccagcttt tgattgattt
     5221 ctcttccctt attacttcca ccagtcatgg cattgatata aaaaggaaag tcccactttc
     5281 gaccagcaaa ctctgtcgaa agatcgattt catccagatt gtaaagaggc aaggaagaat
     5341 gaatcagctc cacctcatca aagctattat aggaactttt ctgctcaagg gcatagagga
     5401 tatgctcgtc cttacgattt gtcgtcatgt cctatccttt cttgatataa gagctcaatc
     5461 cccagatcgg cccaacgatt ttttaaggtt ttggttgatt gcgcatcaaa actcagggcg
     5521 atgccacagt caccaccacc agcaccacta ctcttggcaa cggcctgcaa atcttgactg
     5581 gcttctttca actgtctaag caaaggcgtg taaatatctg tactcaagcc ttctaaaagc
     5641 ttgctggcta cttctacttg ctcgataatc ttttctgatt tcccctgttc caaggcttcg
     5701 accaaagaaa ccaccgtttc ttttgaggaa cttaaaaaat tttgattgat attttgcttg
     5761 atttgctgga ccatgtgact cgatacagcc acttccttgg tccatcccac taagaaatca
     5821 cattctaaag ttggtttcac ttgtgaaatt gaaaagcccc aatcacgctc cagaactgtc
     5881 gccaagtttt cttcttctaa ccaagcagcc accttctggc gatcaaatga ctggtagaga
     5941 accaaatcct ctgccacaat acaggcaagg tcgcccatgg aaccattgtc tcctcgcttg
     6001 agcaagacag cgctagtcag cttgaacaag agctcctgat caacagaaac atcatacaga
     6061 gccagtaaag ccttgacaac caagacaacg acgctgccac tagaacctag accaaacttt
     6121 ttcccttctc gttccatttt gccacagatt tctagggaaa aagatcttaa attctgaccg
     6181 cgaacagcga ggaagtctcc catcaaagca atcgtttctt gaatcaagct gtagtcagga
     6241 ttgggcgtta agtccactgc gaaatcaaac atatctgaat agatacggta gctgtcagaa
     6301 aaagcaatct cagccctcat atagatggga atatccttta tcaaagctaa ctgccctggc
     6361 tctaaaatag catattcacc tgcccaatag agttttccgc aagttttaac agcaatcatc
     6421 ttgactcaaa tcctttgttt ttgacacaat caagcgataa cgctgaccga aaatttctga
     6481 caaatgctcc aagtctttct cctgacaaaa gaccttaaca ttgggaccag catccatggt
     6541 aaagtagcag gcctctcctt tctcacgaag ctggcgaaca aagtccatag cctcataaga
     6601 ggcatccgtc agataagaaa aggctggact agcagtcttt gtcgtagcat gcatagccag
     6661 ggcatttttc tccgttaatt ctccaatctt ggcaaaatca ttttccttga gataaatcag
     6721 catatcctga tagtccttct cagactgacg aacccagtcg tcaaaagtcg tcgaggtttc
     6781 cacacaaagt ttcatcccgt cacggctaga gattggtttt ttcttgtcct ctagcaccaa
     6841 cataatcata gctagtttca agtctgtctc tacagagtaa atttctccac tatccttatc
     6901 ccaggctcct agcggtccat aaaaactccg agaagaagag cctgaggcaa acttggcttc
     6961 ctgcgctaac tgacttctat ccaatccaag cttgaaataa gcattacaag ccttgaccag
     7021 ggcggacaaa ccactagaac ttgaggacag gcccgctgca gtaggcatat tgttttgagt
     7081 atcgatacgg acaaagccct caccagctgg acgataacgg tcaataatct tactcatctt
     7141 ggcatgctcg acctcatttt gtagctgacc attgatgtaa aattcgtcag ctattacatt
     7201 ggctggtaaa ggcgacaagg tcgtctctgt atacatattt tccaaagtta gagaaatact
     7261 gctagtagca ggcaccatct ctttttcttt tttctttccc caatatttga taatagcaat
     7321 atttgcgtag gaacgtactg tcacaggctc tctatccatg tctgaacagc tcctttctct
     7381 tctaatcttt ctgctagttc ttgtgcgtgt gtcaaattgg ttaccaaggc tatgatacaa
     7441 cctcctagcc caccaccgct catcttggca cccagagcac catggctaag agtcgtttca
     7501 accaaaaagt ctgcctcagg gctactgact ccaatttctt ttaaatgtaa atgcgcttga
     7561 ctgaggattt gtcccagtcc ttcagcatct ttttgtgaaa tcgcaacttc tgcttgctgg
     7621 gttaattctc ccaaggcatg caaaaacggt agggcatcct tgcccttatt ttgaaccact
     7681 tggatggctt cacgagtatg accataaaca cccgtatcgg caatcaccaa ataggcggat
     7741 aaatccatct caagttctgt aaatcctacg ttcttgataa agcgaatagg ttggtcactg
     7801 agacaggtct tagcatccaa accactagga ttcatatggg caatcatctc agctcgattg
     7861 accaagattt ctagtacatc atgaggcaga tcagcctgat agtagtcaaa taccgcacga
     7921 atggccgcta tgctgatagc cgctgacgaa cccatccccc gtttctcagg gatagccgag
     7981 tcaatctcac aacgaatgca ggcttctgtg atatccaaat actccagtga ggcataaacc
     8041 gccatggaca aggtatcctc ctcataaagg cgccaaggac tctctgcagg aactacctta
     8101 caggtcacct ccacctccaa aagaggcagg gaaatggcag gataaccgta aacgaccgca
     8161 tgttccccta ttaaaattat cttactatgt gcctgaccga caccaacttt ttttgtcatt
     8221 ttttcctttt actagacgaa aaaacgtctt atttttcata caagtattaa ttctttccta
     8281 tctattttat tatattttca caaaaaaagc gattgtttcc attcacaatc gcttctttca
     8341 ttattgaacc cattcgccat tatagttgac agaatagcca tctacggtcg tattcactgc
     8401 caaggcacct gagctataag cgtagtacca tctgccattg acctggaacc aacctgtcgt
     8461 catagaacga cgaaagaaac tccataccgt taagtaaaga ggaaagtcgt gagggatcat
     8521 gcgccattga caacctgttt tagtgacgta caaagtctca ttaacaagta ctcgtttcgg
     8581 ccatttatag gtgcggtgtt tggagaaata gggttcaatc ttagcccatt cttgatcgtt
     8641 taaatcagta tcatatgctt tgcgtgtcat aactctagtt taacattttt tgtgaataca
     8701 agttctaaat aatcgaccac gaaaatttct taagtggaaa acgccttatg aagtatgcta
     8761 cgggaaactt atgcacttaa tttgacaatt caagatgtaa aaatata
//
LOCUS       DAVBHS010000088         8767 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVBHS010000088 DAVBHS010000000
VERSION     DAVBHS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8767
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.088"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            1..450
                     /locus_tag="VQ651_001429"
     CDS             1..450
                     /locus_tag="VQ651_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2754408.1"
                     /translation="MCTILVSIYYKIGLSSSGVYKAVADGEMTVSLSYEDPAVKLLND
                     GANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQEVQDTLGTTTTNRPVR
                     KNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDIQSKQ"
     gene            463..1122
                     /locus_tag="VQ651_001430"
     CDS             463..1122
                     /locus_tag="VQ651_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754409.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIEGGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     K"
     gene            1249..1554
                     /locus_tag="VQ651_001431"
                     /pseudo
     CDS             1249..1554
                     /locus_tag="VQ651_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809465.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
     gene            1544..3189
                     /locus_tag="VQ651_001432"
                     /pseudo
     CDS             1544..3189
                     /locus_tag="VQ651_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(3292..3984)
                     /locus_tag="VQ651_001433"
     CDS             complement(3292..3984)
                     /locus_tag="VQ651_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2754410.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGCEKMEEGSI"
     gene            complement(4228..5565)
                     /locus_tag="VQ651_001434"
     CDS             complement(4228..5565)
                     /locus_tag="VQ651_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HET2754411.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALTETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGAAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     IGDSVARVLEEMGLETVGTHGTTVALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(5575..5841)
                     /locus_tag="VQ651_001435"
     CDS             complement(5575..5841)
                     /locus_tag="VQ651_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET2754412.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(6106..6492)
                     /gene="rplQ"
                     /locus_tag="VQ651_001436"
     CDS             complement(6106..6492)
                     /gene="rplQ"
                     /locus_tag="VQ651_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET2754413.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(6504..7439)
                     /locus_tag="VQ651_001437"
     CDS             complement(6504..7439)
                     /locus_tag="VQ651_001437"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET2754414.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(7482..7865)
                     /gene="rpsK"
                     /locus_tag="VQ651_001438"
     CDS             complement(7482..7865)
                     /gene="rpsK"
                     /locus_tag="VQ651_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET2754415.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(7883..8248)
                     /gene="rpsM"
                     /locus_tag="VQ651_001439"
     CDS             complement(7883..8248)
                     /gene="rpsM"
                     /locus_tag="VQ651_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET2754416.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(8266..8382)
                     /gene="rpmJ"
                     /locus_tag="VQ651_001440"
     CDS             complement(8266..8382)
                     /gene="rpmJ"
                     /locus_tag="VQ651_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET2754417.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(8407..8625)
                     /gene="infA"
                     /locus_tag="VQ651_001441"
     CDS             complement(8407..8625)
                     /gene="infA"
                     /locus_tag="VQ651_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET2754418.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
BASE COUNT         2627 a         1976 c         1506 g         2658 t
ORIGIN      
        1 atgtgtacta ttctagtttc aatatactat aaaatcggtt taagttcatc tggtgtctat
       61 aaagcagtcg ctgatggaga aatgactgtt agtctctctt atgaagatcc agcagttaaa
      121 ctcttaaatg acggagctaa cattaaggta gtctatccaa aagaaggaac cgtcttccta
      181 cctgctagtg ctgctatcgt taaaaaatct aaaaatatgg aaaatgccaa gaaatttatc
      241 gattttatta tctctcaaga agtacaagat acacttggta caaccactac taaccgtcct
      301 gttcgtaaaa atgctaaaac aagcgaaaac atgaaaccaa ttgacaaaat caaaacactc
      361 actgaagatt atgattatgt catcaagaat aaatcagata tcgttaagaa atacaacgaa
      421 gtctttacag atatccaatc taaacagtaa aagaggttca ctatgagtga gatcgaaatt
      481 attaacgcca aaaaaatcta ccacgatgtc cctgttattg agaatttgaa cattacaatt
      541 ccaaaaggaa gtctctttac ccttcttgga gcttcaggat gtgggaaaac gacccttctt
      601 cgtatgattg caggtttcaa cagtatcgaa ggtggagaat tttacttcga tgatacaaaa
      661 atcaataata tggaacccag caaacgcaat atcgggatgg ttttccaaaa ctacgctatt
      721 ttcccacatt tgactgtccg agacaacgtt gcttttggtc ttatgcaaaa gaaggttcca
      781 aaagaagaat tgattcaaca gaccaacaag tatcttgaac tcatgcaaat tgctcaatat
      841 gcggatcgaa agcccgataa actcagtggt ggacaacaac aacgtgtcac cttggcatgc
      901 gccttagcgg ttaatccaag tgttctcctc atggacgagc cacttagtaa tctggaggcc
      961 aaacttcgct tggatatgcg tcaagccatc cgagaaatcc aacacgaagt gggaattaca
     1021 actgtttatg taacccacga ccaagaagaa gccatggcta tttcagacca aattgctgtt
     1081 atgaaagatg gggtgatcca acaaatcggc cgaccaaaat aactctatca taaaccagct
     1141 aatgagtttg tggcaacctt tatcggacgc acaaatatta tccctgccaa tcttgaaaaa
     1201 cggagcgacg gcgcttatat cgtcttttca gatggctatg cccttcgaat gccagctctt
     1261 gatcaggttg aggagcaagc tattcatgta agcattcgtc ccgaagagtt tatcaaagat
     1321 gaatctggag atatcgaagg aactattagc gatagcgtct atcttggact aaatacggag
     1381 tatttcattg agacaggttt tgcctcaaaa atttaagtta gtgaagaatc aacttttgaa
     1441 gaagaactac aaaaaggcaa tcgtattcgt ctacgaatca atacgcaaaa attaaacatc
     1501 ttttctgcag atggttccca aaacctgata aaaggagtca accatggaat gtaaaaaact
     1561 aaatatttgg acagcttcct ctttcttcat ctttcttacc tatcttgtct ttctcgttta
     1621 tcctatcgtt accgtgctca agcaagcact tatacatgaa ggacaattct cactagctaa
     1681 ttttgtcact ttctttagta aagcctacta ctctgagaca ctagtcaaca gtttcaaggt
     1741 ttccattacc gctactatca cttccttagt tgtaggaacc ctattagctt atctcttctc
     1801 tatgtatgac ttcaagggga agaaatttct acaaatattg attatcattg cttccatgtc
     1861 agctcctttc gtaggagcct actcctgaat tctcttgctg ggacgaaatg aggtcatcac
     1921 taaatttttg acaaatgccc ttcatcttcc agctatcgat atttatggat tcaaaggaat
     1981 tgtacttgtc tttacactgc aactattccc actggtattt ctatatgttg ctggaacaat
     2041 gaacagtatt gacaattctc tacttgaagc agctgaaagc atggggtcct tcggatttaa
     2101 gcgtatcgta acggttgttt tacctctcct agttccaacc ttactagcag ctccttgctt
     2161 gtatttatga gagcattctc agactttgga acgcctatgt tgattgacga aggatatcgg
     2221 actttccctg tcctgattta tacccaattt attagcgagg ttggaggaaa ttctgctttt
     2281 gcaattatgg cgattatcat tgccttggca attttcctta tccaaaaaca cattgcaaac
     2341 cgctacagtt tcagcatgaa tctgctccat ccaattgagc ctaaaaaaac tacaaaagga
     2401 aaaatagctg ccatttatgc aacagtctac ggaattatct ttatctctgt tttacctcaa
     2461 atctacttaa tttatacctc tttcctaaaa acatcaggta tggtatctgt taaaggttat
     2521 tctccaaaca gttacaaggt agctttcaat cgtatgggat ctgctatttt caataccatt
     2581 cgtatccctt tgattgcctt agttctagtt gttctatttg cgacatttat ctcctaccta
     2641 gccgttagaa aacggaattt gtttacaaac ttaattgaca gcctcagtat ggtaccttat
     2701 attgtaccag gaaccgttct agggattgcc ttcatttctt ccttcaatac tggtctattt
     2761 ggaagtggat ttcttatgat tacagggact gctttcatct tgattatgtc tctatctgtc
     2821 agaagattac cttatactat tcgctcatct gttgctagct tacaacaaat agcaccaagt
     2881 attgaagaag ctgctgaaag cttaggaagt agtcgtctca atacctttgc taagattaca
     2941 actccaatga tgctatctgg tatcatttct ggagccatct tatcttgggt cacaatgatt
     3001 tcaaaactct ctacttctat cctcctctac aatgtcaaaa caagaacaat gactgtagct
     3061 atttatacag aggttctcag aggaaattac ggtgtagccg cagccttgtc aactatcctg
     3121 actgttctaa cagtaggttc cttgctcttg tttatgaaaa tctctaaaag caatagcatt
     3181 acactttagt tttccacatc aaaaacagcc gaaaggagtg ccctcggctg ttttttctta
     3241 tcttgatatt cttatcaaat ccctagctca tggcaagcaa gtctagactg attaaataga
     3301 gccttcttcc atcttctcac atcctagctc tcggtaacta cggtcaccga caacttcaac
     3361 cctaaactga ccgtcctcat attcaagaat cgtcacgcta ccattatcta gaccatgcgg
     3421 atgcatgcca ttaatcagat aaacaatggt tccaatagtc attccatggc tgacaacaag
     3481 ggcgttacct ccaccttgat cttccatttc ttttgcaatc atttcaaagc cttccttgat
     3541 tcggccactg agtttttccc agccttcagc ccaaccagct gtatcgacct ctaccaagcc
     3601 ctcagccagt tcagcataag acaattggtg aacgtggtcc acattaaaga tacgaggaat
     3661 aatgcccatg aaaagatcgc catcataggc tccatcaaaa ctaccgaaac accattctct
     3721 gatacgcttg tccatgcgat aagggatttt cccctgcaag ccaagttctt caaggataat
     3781 tcccatggtc tgaatggtac gaccagaatc actcgaataa gcgcgctcaa actgtagatc
     3841 agattctcgc aaaccgattc ctaactcttg aatccctcgt tcaccttcag cagttaaggg
     3901 agtatcgctc caaccttgcg cgcgaccaat cgtgttaaac atggtcttgc catgacgtac
     3961 caaatacaat cgtactttta ccattttccg tcctccgttt gcttctatta tatcatgaat
     4021 gataaaataa aagccaccca tacaggcgac ttttgaagga gattattatg aaaaagttta
     4081 ggagttcatt aaataaagat attagatgaa aatcaaattc aaactaattc agtgttatct
     4141 gtttcaaata atattaggag gtctttattt aatgattata gtatacacat ctaatcttaa
     4201 atagaactta aaatttctcc tattttctta atttttaaaa ctatgaattg gtgctgggat
     4261 ttgtccaccg cgagagatga agtcgacaga cgaagcccca ttaaccttca taacaggtgc
     4321 agttcctaat agaccaccaa actcaatcat atcgccttct cttcctttgg gaatgatacg
     4381 aacagctgtt gttttcatgt tgataacacc gattgctgct tcatccgcaa tcatagccgc
     4441 aatggtttca gcaggcgtat cttctgggat ggcaatcata tccaatccaa cagaacagat
     4501 agccgtcata gcttctagtt tttctaaatt aagagagcca ttttgcactg cagcaatcat
     4561 tccttcatcc tcagaaacag ggataaaggc gccagataaa ccaccgactt ggttgcaggc
     4621 catcactcca ccctttttaa cttggtcgtt caagagggcc aaggcaaccg tcgttccatg
     4681 cgtgccaact gtttctagcc ccatttcctc aaggacacgt gccacagagt ctccaatcgc
     4741 aggggttggt gccaaactca agtccacaat accaaactcc acacccagtc tctcactggc
     4801 catttgacca accaattgac cgatacgagt gattttaaag gcagttttct taactgtttc
     4861 ggctactaca tcaaagctct gtccacgaac tttttccaaa gcacgtttca ccacaccagg
     4921 accagaaact ccgacattga tgataacatc tgcttcccca acaccatgaa aggcacccgc
     4981 cataaatgga ttgtcctcaa cagcattagc gaatacaacc aacttagccg ctcccatatc
     5041 tgaaagattt gccgtttcct tgataattcg tcccatatct gccacagccg tcatattaat
     5101 accagacttg gttgagccga tattgactga cgagcagacc ttatccgtct cagtcaaagc
     5161 gcgaggaatg gaattgatga gaatctcatc tcccttttga taaccttttt gtactaaggc
     5221 agaaaaacca ccaataaagt ccacaccaat ctctttcgca gccttatcaa gcgcttttgc
     5281 cagaaccacg tagtccgtcg catctgtcgc tgccccaatc agagaaatag gtgtcaccga
     5341 tacacgctta ttaacgatag gaattcccaa ctcagccgca atttcatcac caacagctac
     5401 taaattagcc gcctttgtcg taattttttg atagattttc tccgcagcac gattgatatc
     5461 tggatcgata cagtccaaaa gagaaatccc catggtaatg gttctaatat cgaagttttg
     5521 ctcctcaatc atggcgatgg tttcagtaac ttgtctaata tccatgatga cctcctagat
     5581 attatacata gcttcgaaaa tcgctgcact ctgaatattg atttttacat tcaaagtttg
     5641 cccaaaagct tcaaattcat tacgaagata ggtaaaatct tgcttttcat cactagatac
     5701 aacagccatc atcgtaaaat attcatccaa gacagtttga gagatatcgt caatattcaa
     5761 tcctaattct gcaattttac cagaaacacc tgcaacaatt ccagatttat ctttaccaac
     5821 aacagttata atggctttca taagtaaact ccaattcttc ttaaaataag aaaccttaac
     5881 attaaacagg attcttttca aatagtatag cataattcta acgaaaatac tcaaaaacgc
     5941 ctgcttacga agttgttctt aagaaaaaaa caatttcgta aacaagcgtc tactatccca
     6001 acttatgatg agtgttcccg ctaggaccga aatcctagcg gtagaccaga gctagactaa
     6061 gagcacaaga ctccatcatc ataacactca acaaaattga tgattttata ctaattcgat
     6121 gatcgccatt ggcgctgcat caccacgacg tggttcagtt ttaaggatac gagtgtatcc
     6181 accgttacgt tcagcataac gaggtgcgat ttctgagaac aatttttgaa gtgctgtagt
     6241 agaagtgtac ttatcagttg cttcatcata gttttcagat gcgatttcat tacgtacgaa
     6301 agcagctgct tgacgacgtg catgcaaatc accacgttta cctagagtaa tcattttttc
     6361 aacagtttta cggatttctt tagcacgagc ttcagttgtc acgattgatt cgttgatcaa
     6421 aaggtcagtt gtcaaatcgc gaagcattgc tttacgttgt gagctagtgc gtcctagttt
     6481 acggtaagcc atgtattcct cctttattta tcttttaatc caagacccaa atcaatgagt
     6541 ttgagtttca cttcttccaa actcttgcgt ccaagatttc gtactttcat catctctgct
     6601 tcagattttt ctgtcaaatc atgcacagta ttgataccgg cacgttttaa acagttgtat
     6661 gaacgcacag acaagtccag ttcctcaatc gtacgatcta aaatacggtc gtcagattca
     6721 gtatcagctt ctttcatcac ttcagttgac ttagcaatct cagtaagatt tgtaaacaaa
     6781 tcaagatgtt ctgtcaaaat acgtgctgaa agccctaaag catcttctgg aataattgtt
     6841 ccatttgtca agatttcaag ggttaatttg tcgaaaccat cattgctacc tacacgagca
     6901 ggttccactt gatagttgac ttttgtaact ggtgtataaa tagaatctac agcaagtgtt
     6961 ccaactggtg cattatcctt tttattttca tcagcaggta catatccacg accactgtta
     7021 acagtcatag tcgcttttag agaagaacct tcaccaattg taaagagata atgatctgga
     7081 tttacaattt caatatcgct atctgtcaaa atgtcaccag ctgttacttc agcaggacct
     7141 tcaacatcca gttcgatgat tttttcgtct tcaacgtacg atttcactgc aattccttta
     7201 atgttcagaa tgatttgcat cacgtcttca cgaacacctg gaactgtgtc aaactcatgt
     7261 aacacaccat caatgttgat agatgtcaca gctgctcctg gtagagaagc tagaagtaca
     7321 cgacgaagag agttaccaag agttgtaccg tagccacgtt caagtggttc gattacaaac
     7381 ttgccataat ctttattttc atcaattttt gttatatttg gtttttcaaa ctcgatcatt
     7441 tagttactcc ctcttaaacg aaaagcagtg taatgcgatg attatacacg gcgacgtttt
     7501 ggaggacgag caccattgtg tggcactgga gtcacatcac gaattgctgt tacttcaaga
     7561 ccagcggcag caagcgcacg aatagctgac tcacgaccag aacctggacc ttttacagta
     7621 acttcaactg atttaagacc gtgttcttgt gcagatttag cagcagcttc agaagccatt
     7681 tgagcagcga atggtgtaga tttacgagaa cctttgaaac caagagcacc agctgatgac
     7741 caagcaattg cattaccatg cacatcagta atcataacaa tagtgttatt aaatgtagcg
     7801 tgaatatgag caataccaga ttcgatattc tttttcacac gacgtttacg tgttggttta
     7861 gccaagactt ttacctccta tattattttt tcttaccagc aatcgcaaca gctttacctt
     7921 tacgagtgcg agcgttgttt ttagtatttt gtccacggac aggaagtcca cgacggtgac
     7981 ggataccacg gtatgaaccg atttccatca aacgtttgat gttcaagttt acttcacgac
     8041 gaaggtcacc ttcaactttg attgcatcca cttcacgacg gatagcatct tcttgatctg
     8101 atgtaagatc acgtacacga acatcttctg agattccagc agcagccaaa attttcttag
     8161 atgttgcaag tccgatacca taaacataag tcaatgagat tactacgcgt ttgtcatttg
     8221 gaatatcaac tccagcaata cgagccatgt tttctccttt ctatcttatc cttgacgttg
     8281 tttgtgtttt ggatttgctg ggcaaattac cataacacga ccattacgac gaataacttt
     8341 acagtattcg caaattggtt tgaccgatgg tcttactttc atttcttatc cctccaagtt
     8401 tttcgattat ttaaagcggt aagtgatacg tccacgtgtc aagtcatatg gactcatttc
     8461 gacagtaaca cgatctcccg ctaaaatacg aatatagttt ttacgaattt taccagaaac
     8521 tgttgctaaa atctgatgtc cattttcaag ttcaaccgta aacattgcat tcggcattgt
     8581 atcaactact ttgccttcaa cttcaatcac atcgtctttt gccacgcaaa agcacctcca
     8641 taaatttcga ttcgatgcct ctagacacag agacaacaat tataagtcag actatctcag
     8701 tataacattt gtagcggatt tttgcaagtg cgaaaaacgc tttatttcaa atttgtcaat
     8761 acttttt
//
LOCUS       DAVBHS010000089         8701 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVBHS010000089 DAVBHS010000000
VERSION     DAVBHS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8701
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.089"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            20..199
                     /locus_tag="VQ651_001442"
     CDS             20..199
                     /locus_tag="VQ651_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754419.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            336..515
                     /locus_tag="VQ651_001443"
     CDS             336..515
                     /locus_tag="VQ651_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HET2754420.1"
                     /translation="MPMTQKEMVKLFTAHGWIKTRGGKGSHIKIEKQGERPITILHGE
                     LNKYTERGIGKQAGL"
     gene            552..1004
                     /locus_tag="VQ651_001444"
     CDS             552..1004
                     /locus_tag="VQ651_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET2754421.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            1295..1765
                     /locus_tag="VQ651_001445"
     CDS             1295..1765
                     /locus_tag="VQ651_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HET2754422.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     VLFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            1784..2872
                     /locus_tag="VQ651_001446"
     CDS             1784..2872
                     /locus_tag="VQ651_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET2754423.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            2853..3281
                     /locus_tag="VQ651_001447"
     CDS             2853..3281
                     /locus_tag="VQ651_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2754424.1"
                     /translation="MELEISDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPGGG
                     REGDESPFECARREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSVFMVGQLRQEQFDN
                     IIFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            3419..4369
                     /locus_tag="VQ651_001448"
     CDS             3419..4369
                     /locus_tag="VQ651_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET2754425.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYMGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDMTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNTG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDKW
                     DMVRQSAESAGFFLETHMIQGEWNACVFKKTKDISGVIGG"
     gene            4371..5114
                     /locus_tag="VQ651_001449"
     CDS             4371..5114
                     /locus_tag="VQ651_001449"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET2754426.1"
                     /translation="MQQYFVKGSAVSPVTIEDRETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFITQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNVVPSIHLFENKADFLAQLDQ
                     FDRILVAYEEAAKEGEAAALIKAVTGLEKGAKLLFIFGPEGGLSPAEIENFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            5125..6921
                     /gene="pepF"
                     /locus_tag="VQ651_001450"
     CDS             5125..6921
                     /gene="pepF"
                     /locus_tag="VQ651_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET2754427.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDATLTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIISYSAELGE"
     gene            7167..7721
                     /locus_tag="VQ651_001451"
     CDS             7167..7721
                     /locus_tag="VQ651_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HET2754428.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRNVLLNQGL
                     HYLSKEKDTRGFSSQYGWVHAFAHGADLLTEVVCHPGFPKNRVHEVFEILGQLFKRMS
                     IRFTDDEDWRLARVIYEPILQGKLAQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDELKEAIQSFQY"
     gene            7832..8500
                     /locus_tag="VQ651_001452"
     CDS             7832..8500
                     /locus_tag="VQ651_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET2754429.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLSN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT         2493 a         1558 c         2086 g         2564 t
ORIGIN      
        1 aaggcgacgt tgacgtggtt tgaatttgat tttcgaagag tatcagtttg taaaaattgg
       61 agataaaaaa tatagagtac gcagtggtcc tatgattcat tcaactccgc ctcaatctgt
      121 cttagatcga gatactttta caattgacta tacagatgat gaattgctcg ataaagaagc
      181 agtttttact actcattaga aaaataggtc tgaataaatt ggtttccctt agaaccacat
      241 ctaggggctt ttatgtataa tagaataata agtatcactc ctttacaata cgtgtaacac
      301 gtgttataat ggtgttgtca ggaggtaaaa gcgctatgcc tatgacacaa aaagagatgg
      361 ttaaactctt tacggcccat ggttggataa agactagagg cggtaaaggc tcccatatta
      421 aaatcgagaa gcaaggggaa agacctatca caatccttca tggtgagctg aataagtaca
      481 ctgaaagagg gataggaaag caagctgggt tgtaaaattc agtccctgct ttttgatatg
      541 gaggtagtga aatgttagtt acgtatccag ctttatttta ttatgacgac acagacggaa
      601 cagaagcgac ttattttgtc catttcccag attttgaata ctcagctaca caaggagagg
      661 ggatttctga ggctttggct atggggtcgg agtggctagg gataactgtt gcagatttga
      721 tagagagtga tggagagtta cctcagccat cagatatcaa tagcttgtct ttaattgata
      781 atgatccttt taaagatgat gaagatttcg tgtcaaccta tgaccttgat aaatctttta
      841 tttctatggt atctgttgat gtatcagaat acctaggaag tcaggaaccc attaaaaaga
      901 ctttgaccat accaaaatgg gcagataagt tgggacgaga aatgggactt aacttttctc
      961 agactttgac agacgctatt gcagataaga aagttcaagc ctaagattaa gcgtggtatc
     1021 gtggtaatag tcaataaaaa agcacgtcca cttgtgctag ttactcgcct attgatttta
     1081 tagatttatt agccctttcg agggcttttt atattgattt ttatgagaaa tgaagaaatc
     1141 agacttttaa gaaaatcact gataccaagg gtttaaatga ggtaatatgg tataattagg
     1201 acataaaata attttgtggt aagatggtag tatctatttt agcatatttc cgagcaatcg
     1261 gggcgattaa agagtcgcat agaaagagga caaaatggca acatacggat ttttagatgt
     1321 tttagaggaa gagttggaca agaactttcc ctttgacttt gagattagct gggacaaacg
     1381 caaccatgcg gtagaagtga gttttctatt ggaagcgcaa aatgctgcag gtgtggagat
     1441 ggtggatgaa gacggagagg tttcatcaga tgacattctt tttgaggaag cggtcctttt
     1501 ctacaatcct gctaagtcaa cagtcaatga ggaagactat ttgactgtta ttccttacct
     1561 gcctaaaaaa ggattttccc gtgaattttt agcttatttt gtgcttttcc ttaaagatac
     1621 tgctgaggtt gggctagatg tcctcatgga ctttttggaa gacccagaag cagaggaatt
     1681 tgtcatggaa tggaaccaag aagtctttga agaaggaaaa gttggcttgg aagagggaga
     1741 attttatcct tatccaagat actaggagtt ggttggaggt gttatgaaga aaattgggac
     1801 ttatttggtt tatgtgctag ctgttgtctt tattatgcta gcttttgctt gtggaacaat
     1861 cgcatttgca gagttggggt attccgcagt tttagccttt acttttggtt atgccttcgc
     1921 tcttctaagc atgtatttaa tctttattct tcatgagctg ggtcatgctt tttgtggcta
     1981 cttgacaggc tatcggctgg tggcttttgg attaggacat tttattttga ccaaaaagtc
     2041 aggcaggttt catcttagta gaacagccat tctgaaaaat gttggtgctc aatacattgg
     2101 tttaaaagag gatgaaagcg atcaaagaat catcctgatg ctttcaggag gcttgatggt
     2161 tcatctcggc ttgatattat tggcgatagt gtttggattt ttgacaagaa gctggtattt
     2221 tgcagggact tggatttttc ttaatttatc tttcttccta aataacattt tgccagtcga
     2281 catcaccgat ggagcaaaaa tttgggaatt actacaacac cctgaaaata ccaaatacgc
     2341 ctacctggta ttgagacatt ctgctcaaac tttgctggct cctcaagaat atgatttgaa
     2401 agactttgtt caagcagttc ctgaggatgc acgagggagt tttgcggatg gtgtattagg
     2461 tatgcaaggt gaagtttcca ttttggaagg gaaggaagaa gtagctaaac agcagtttca
     2521 agcattactt gagaggacaa ctactccgat gatgcagaca gttgcccaac tatccctgct
     2581 tcatatcgcc ttgttggaag gagattttga gcaagcggag caatatgcta gtattcgaca
     2641 ggtcaaatcg tttctgtctc tcaaactgtc caatcttcaa gtgatacaag cctggtatca
     2701 attaaaggtg aagaaagatg tagatcgaac tcgtaaggct ctcaagattg ctagacagaa
     2761 aatggatagt agtcgaatgt tgagggacga gaaacgctat tatgaaaact tgttagcaga
     2821 gctggaaaag gaaatgttag aaggaactga agatggaact agaaatttct gatttccaag
     2881 gttgcaagat tgccctcttt tgtggggata agctactgac gattttgcgt gatgacaagg
     2941 acgatatccc ttgtcccaac atgtgggagt tgccaggtgg tggccgtgaa ggggacgaaa
     3001 gcccttttga atgcgctcgg cgtgaagttt atgaggaact gggaattcat ttagatgaag
     3061 actgtctgct ttggagcaag atttatccta gcgtaatttt taaaggcaag aaatcggtct
     3121 ttatggttgg tcagctaaga caagaacaat ttgacaatat catcttcgga gatgaaggac
     3181 agggctatca gctgatgaat gttgaggaat ttcttagttc cagtcaagtt gtgcctcagt
     3241 tgcaagagag attaaaagat tatttaaaag taagtgatta gaaaggatgg ttcagttaca
     3301 tttctaaact gaacccgccc taaacactgt gccaaaaaga taaacttctc ttagacacaa
     3361 gcgtcttcag agaatttcct attttggttt tgtgttttac gggcttggta tcttaataat
     3421 ggaaacatgg caagagttaa aagttacagt gaagcgtgag ggagaggagt tggtttccaa
     3481 tctcttgatt gagctgggag cgcaaggagt cgcgattgaa gacagcatgg actatatggg
     3541 aaatgtggat cgttttggcg agattttccc agaggtggag cagcaagaag aaatcgtagt
     3601 gacagcctac taccctgaca cggtggatat gacagtggtt gaggcggact tgcaggctcg
     3661 cttagcagaa ttgacggatt ttatggattt gggagagtta aaaataggta cgactgcctt
     3721 ggctgaggaa gactgggcag acaactggaa gaaatactat gaacctgctc gcatcactca
     3781 tgatttgacc atcgttccct cttggacaga ctatgaggcg actgcgggag aaaaaattat
     3841 caagctggat cctggcatgg cttttggtac tggaacccac ccaactacca agatgagcct
     3901 ttttgccttg gaacaggttc ttcgtggtgg cgaaacggtg ctagatgtgg ggactggttc
     3961 aggggttctt tctattgcaa gctcactcct aggtgccaag gagattttcg cctatgactt
     4021 ggatgatgtg gcggttcgtg tcgctcagga aaatattgag ctcaacactg gtatggaaaa
     4081 tatccatgta gcagcaggag acttgcttaa gggagttgag attgaagcag atgtcatcgt
     4141 ggctaatatc ttggcggata ttctcattca tctgacggag gatgcttatc gcttggtaaa
     4201 ggatgaaggc tacctcatca tgagtggcat tatcaaggac aagtgggaca tggtgcgcca
     4261 gtcggctgag tcagctggat ttttccttga aacccatatg attcaagggg aatggaatgc
     4321 ctgcgtcttc aagaagacca aggatatttc aggtgtgatt ggaggctagc atgcaacagt
     4381 attttgtcaa ggggagtgca gtctctcctg tcaccatcga ggacagggaa accagcaagc
     4441 atatgtttca ggttatgcgc ttgaaagaag aggatgaggt taccttagtc tttgatgatg
     4501 gcatcaagcg cttggcgcgc gtgctggata tggaaaatcg tcagtttgag ttggtccaag
     4561 aattaaatga caatgtggaa ctaccagtcc aagtgaccat cgcatctgga tttcccaagg
     4621 gagataagtt ggagtttatc actcaaaagg tgacggaact aggtgccagc cagatttggg
     4681 ccttccctgc cgattggtcc gttgctaagt gggatggcaa gaaattgggt aaaaaggttg
     4741 aaaaactaga aaaaattgct cttggagcag cagagcaaag taagcgtaat gttgttccaa
     4801 gtatccatct tttcgagaat aaagcagact ttctagccca acttgaccag tttgaccgca
     4861 tcttagtagc ctatgaagag gcggccaaag aaggagaagc cgctgctctt ataaaagcag
     4921 ttactggact cgagaaagga gccaaattgc tctttatctt tggtccagaa ggtggtctgt
     4981 cacctgcgga aattgaaaat tttgaagcta agggagctgt tttggcagga cttggccctc
     5041 gcattctaag agcagaaaca gcaccgcttt acgccttatc agcccttagt gttttagtag
     5101 aattagagaa ataagaggaa gaaaatggaa caaaaacacc gttcagaatt tccagagaag
     5161 gaactttggg atttaacagc tctataccaa gaccgtgagg atttcttgcg tgcaatcgag
     5221 aaagctcgcg aagacatcaa ccagtttagc cgtgattaca agggcaatct tcacactttt
     5281 gaggatttcg agaaggcctt tgcggaattg gaacagatct acattcagat gagccatatt
     5341 ggcaactatg gttttatgcc tcagacgacg gactatagca atgacgaatt tgcaaatatt
     5401 gctcaagctg ggatggaatt tgaaacagat gccagcgtag ccttgacctt ttttgacgat
     5461 gccttggtgg cagcagacga ggaagtcttg gatcgtttgg gtaaattgcc acatttaaca
     5521 gctgccattc gtcaggctaa aatcaaaaaa gcccactact taggggcaga tgtggagaag
     5581 gccttgacaa atctcggtga agttttctac agtccgcagg acatttatac taagatgcga
     5641 gctggggatt ttgaaatggc tgactttgaa gcccatggca agacctacaa aaacagcttt
     5701 gtgacctatg agaatttcta ccaaaaccat gaggatgctg aggttcgtga gaaatccttc
     5761 cgttccttct cagagggact tcgtaagcac caaaatacgg ctgcagcagc ctatctggct
     5821 caggtcaagt ctgaaaaact cttggctgat atgaagggat acgactctgt ctttgactat
     5881 cttctagctg aacaagaagt ggaccgtgtc atgtttgacc gccagattga cctcatcatg
     5941 aaggactttg caccagtcgc tcagagatac ctcaagcatg ttgccaaggt aaatggtctt
     6001 gaaaagatga cctttgcaga ctggaaattg gacttggaca gcgccctgaa tcctgaagtg
     6061 actattgacg atgcctatga tttggtcatg aagtcggtag aacctttggg gcaagaatat
     6121 tgtcaggaag ttgctcgtta ccaagaagag cgctgggtgg actttgctgc taacagtggc
     6181 aaggattccg gtggttatgc ggcggaccca tatcgcgtac acccttatgt actcatgagc
     6241 tggacaggcc gtttgagcga tgtctatacc ttgattcatg aaatcgggca ttctggtcaa
     6301 ttcatctttt cagacaatca tcaaagttac ttcaatgccc atatgtcgac ctactatgtt
     6361 gaagcaccgt caaccttcaa tgaattgcta ctcagtgatt acttggagaa ccagtctaat
     6421 gacccacgtc aaaaacgctt cgctctggct catcgcttga cagacaccta cttccataac
     6481 tttatcaccc acctcttgga agccgccttc cagcgtaagg tgtatacatt gattgaagaa
     6541 ggggagacct ttggagcaag caagctcaac agcattatga aggaagtttt gacggatttc
     6601 tggggagatg ctattgaaat tgacgatgat gcaactctga cttggatgcg ccaagctcac
     6661 tactatatgg gcttgtatag ttacacttac tcagcaggac tagttatctc gactgctggt
     6721 taccttcatc taaaacattc tgaaactgga gctgaagact ggctcaatct cctcaaatca
     6781 ggtggtagca agacaccact tgagtcagcc atgattatcg gtgctgatat ttcaacagac
     6841 aaaccactcc gtgataccat ccaattcttg tctgacacag ttgaccagat tatctcctat
     6901 agtgctgagt tgggagagta ggggaataga aaaatttttt agaatgatgt ttatgggctt
     6961 gttaagcgtc attctatttg tcttagttgt agctattttc aaaaatgtcg ttgaagatcg
     7021 tgatgttaaa tttgaactta ctgtagaact ggtattgata gttatttacg tcattttata
     7081 tcagattgta tttaattgag aattcaatat tgagtcattt aagactgcct ttttgaacag
     7141 ttgctagaaa tgtagaggta ttgaccatgt atcaagattt actaagaaaa atagcagaag
     7201 aaaaaccaaa ttataaccag gaagaaatcc agtggttgtt tgatcatttg ggaaatcctt
     7261 ctccagaaat tcgtaatgtc cttttaaatc aaggcttgca ctatctttca aaagaaaagg
     7321 atacaagggg tttttcaagt cagtatggtt gggttcatgc ttttgcacat ggagccgatt
     7381 tactgacaga ggtggtttgt catccgggct ttcctaaaaa cagagttcat gaagtatttg
     7441 agatacttgg ccaattattt aaaagaatgt cgattcgctt tacagatgat gaggattggc
     7501 gtttagcaag agtgatctat gaacctattt tacaagggaa gttagcgcaa gaacaagtag
     7561 cttcttggat aaaaactgtt gattttccga tagaagaaag ggaggatttt tacaaatttt
     7621 ccaactttag atcctgtctg gtggaagtct atgtccaact tgaccagaga aatagtttac
     7681 aagatgagtt gaaagaagct atccagtctt ttcaatacta gagattagct aatagttcta
     7741 tgattttcaa aaaatttcca atcaattttc ttgaaaccct ttcctacttt tgatagacta
     7801 gtatctagtt tttgaaaaaa ggagaaagaa aatgaaaaaa tttgttgctg agttaatcgg
     7861 tacgttcatg cttgtgttcg ttggaacagg agctgttgtt tttggaaatg gtcttgatgg
     7921 ccttggtcac cttggaatcg cctttgcctt tggtttggca atcgtggtgg cagcctactc
     7981 aatcggaact gtttcaggtg ctcacttgaa cccggctgtt tcgattgcta tgtttgtaaa
     8041 caaacgtttg tcatcttcag aacttgtaaa ctacatcctt ggtcaggttg ttggagcttt
     8101 catcgcttct ggcgcagtct tcttcctctt gagtaattca ggtatgtcaa ctgctagtct
     8161 tggtgaaaat gccttggcaa acggtgtcac tgtctttggt ggtttcttgt ttgaagtcat
     8221 cgcaactttc ttgtttgtat tggttatcat gactgtgact tcagaaagca agggcaatgg
     8281 cgcgattgct ggtttggtaa tcggtttgtc attgatggcg atgattcttg tcggattgaa
     8341 gattactgga ctttcagtaa acccagctcg tagcttggca ccagctgtct tggtaggcgg
     8401 cgcagccctt caacaagttt ggattttcat ccttgcacca atcgctggtg gagttcttgc
     8461 agcccttgtt gcaaaaaatt tccttggaac agaagaataa ttgaaactca aaaagccttg
     8521 ctcctcatct tgaggaacag ggctttttcg tatgatactc ttcgaaaatc tcttcaaacc
     8581 acgtcagctt taccttgccg taggtatggt tactgactac gtcagttcta tccacaacct
     8641 caaaacagtg ttttgatctg acttcgtcag ttttatctac aacctcaaac actgctttga
     8701 g
//
LOCUS       DAVBHS010000090         8598 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVBHS010000090 DAVBHS010000000
VERSION     DAVBHS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.090"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            264..1322
                     /locus_tag="VQ651_001453"
     CDS             264..1322
                     /locus_tag="VQ651_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754430.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTVWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNFTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALGDLSKQYLNRNISELQDGSFDGGIQDAVASKQIVVINEFIACLNSRCMPSQYDR
                     FFYKDRPYIFSRKSPIKG"
     gene            1637..2611
                     /locus_tag="VQ651_001454"
     CDS             1637..2611
                     /locus_tag="VQ651_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754431.1"
                     /translation="MKNKRYFFDTILMILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSTK"
     gene            2991..3365
                     /locus_tag="VQ651_001455"
     CDS             2991..3365
                     /locus_tag="VQ651_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HET2754432.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            3573..4475
                     /locus_tag="VQ651_001456"
     CDS             3573..4475
                     /locus_tag="VQ651_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754433.1"
                     /translation="MIRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEGLIKIFPFIFIRRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWQKEDSFESNLHKILMDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            4531..4770
                     /locus_tag="VQ651_001457"
     CDS             4531..4770
                     /locus_tag="VQ651_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET2754434.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACQGICAAAGVLATA
                     AGKIAYCIGYGVLNRGCDINENPYPWDGFISATVR"
     gene            4767..5495
                     /locus_tag="VQ651_001458"
     CDS             4767..5495
                     /locus_tag="VQ651_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754435.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLVINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IRKDFDIKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGKVDNHVLTLI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKEKEYELFKNSK"
     gene            6630..6869
                     /locus_tag="VQ651_001459"
     CDS             6630..6869
                     /locus_tag="VQ651_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754436.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQDGVFILEDFRNILCTMM"
     gene            7419..>8598
                     /locus_tag="VQ651_001460"
     CDS             7419..>8598
                     /locus_tag="VQ651_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HET2754437.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPVASQSKAEK
                     DYDAAVKKYEAAKKEYEDGKAAQKKYEDDQKKTEEKAEEERKASEEEQAANLKYQQEL
                     VKYIRENDPTKKAEAKKAMDEAEKEYKKKQTEFAEVRAKVIPSAEELKKTRQKAEEAK
                     AKEAELTKKVEEAEKKVTEAKQKVDAEHAEEVAPQAKIAELEHEVQKLEKALKEIDES
                     DSEDYVKEGLRAPLQFELDVKQAKLSKLEELSDKIDELDAEIAKLEKDVEDFKNSDGE
                     QAGQYLAAAEEDLVAKKAELEKTEADLKKAVNEPEKPAEETPAPAPKPEQPAEQPKPA
                     PAPQPEKPAEEPENPAPAPQPEKSADQQAEEDYARRSEEEYNRLTQQQPPKAEKPAPA
                     P"
BASE COUNT         2977 a         1163 c         1563 g         2895 t
ORIGIN      
        1 taaatctaat aaattctatt attatagctc ttggtatgat attggcatta attttggtat
       61 tatcaagagt gtatagaaaa tatttttata gagacgatga tgactaaagt ttttatcaat
      121 aatttgggct ccttgtcaac tgtagtggat tgaagaaaag ctaacgtctg gggaagggtt
      181 taaagctttg ctttatcaaa tattgactta cagagttgct ctattgtatt gatgtattct
      241 tagtaaataa tatggtgttt tgaatgaaag atgatcagaa atatttatta gcgggacttt
      301 attcgcttct agttgcaata ttttattttc cattgattga gtccaagggg atttttgtaa
      361 gtatattgat ggcggtttta cttttatatt taatatactt cattgcaaca gtcattcata
      421 ttgtgataat taagtttata agaaaaaaat cttttaagta tttggttcta tatccattta
      481 cttatgatgg gagctggaga tttcaaccta taaacttact ctactttcca gaaatggtga
      541 gagatgtcat tccgattaat ttagttcaag aatattgcca aggacaacct tacggactgt
      601 taaaaaagat gctgaaaaga attcgattgt cacgagagat agctttactc ctggctacca
      661 ttatcgtcta tttttttacg catagaattc tacctctttc agtatttacg ttcatatttt
      721 cttacatatt gctatttgca caatcttatt tgggtggtaa taccgtttgg attggaaata
      781 gaagattaat aatagatgat gaatttgaaa aaatcttgct ttcaaaaagt tatataaaag
      841 aaatatcatc tgcacgatac tcggaatact tgacatgtga atacaaaaac tttacaccaa
      901 tcatattgtt agctattttt gaaaatttgc ttgactccta cttgcttcag aatcagagtg
      961 aggttgactt agatattttt tataaagttt tacctttgct ttataaagaa aaatatacta
     1021 tgggattcaa ttactttgta agtttaaatt atttactata taaggttgga tttttgggaa
     1081 tcatttacga taatgaagca ttaggagatt tgtcaaaaca atatttaaac agaaatattt
     1141 cggaattaca agatggttct ttcgatggcg gtatacaaga tgctgttgcc tctaaacaaa
     1201 tagtagttat aaatgaattt atagcttgtt taaattccag atgcatgcca tcccaatatg
     1261 atagattttt ttataaagat agaccttata ttttttccag aaagagtcct attaaggggt
     1321 gaaaatttgt tacatttgga ttaacaagtg tcctagggat tagaaataac tctagtatag
     1381 ctagatattt ttgagtaact agaaatatca ttaggctacc catatgattg ccagaaaaga
     1441 tggctgatga atcttattta tactgttgca gtaggcattg tctaggataa ataagctatc
     1501 ttgatggggg aaattggaat aattttagta gaaacattaa aggattatgg acaagtagcg
     1561 caggattagg tgatgcaatg tgctaagttg gcttggtgtt agcagtagta ttatttaaca
     1621 atggaaagta gactaaatga aaaataagcg ttattttttt gacacaattt taatgatttt
     1681 actgcttata agcacgatat tttgtgtcag cccagtattt ataaaattgg acatcttagg
     1741 aaccccctct catgccatct taacctttgt attggcgata ccgttattct atattttgtc
     1801 tcaatgctta catactttgc tattactagt ttcctcaatc ttttgtaaat taagaccaat
     1861 ttatttttat tttatatttg tgatcattat aggagctagg aaatactatc gaattctctt
     1921 tcaccaacta atgggattta gtccaggaat agcagtgttt tataaggaga gtcaaacaac
     1981 taagaatctg tttaaatttt actacttctt gtactttaca acactaattt catactattt
     2041 tttctttact tttgtgtatg acaaaccgtt gcttttgcct cttattccat tttctattat
     2101 catagcatta gtacaaaaac tttatagaat tgagaatcaa caattatttc tgttaaaatc
     2161 aaaggtacta acaatactgg aatctaagaa gaattgtgag tttaatcttc aggattatca
     2221 tgaaatttgg aaattgcaat caaaatctga gttgccatgc gtggctttat cttatatcag
     2281 tttgatcaag ccatatcttt cagagagcgt gcgtgaacaa attgatttat tagaagttaa
     2341 gcgatttaaa aaaataaatc atccaatatc actttatggt atgttggatg ttataaaatt
     2401 aaatctatat ttacggcatt ataatgaaaa aaataaatat gaatcaatgt taaagaaaat
     2461 tttagaggta agaccagact ttgttttaat cgagcagaat attgatgata gtttaaatag
     2521 ctcgcaacca ttatctttat ctttagccat ttcagaaatc caacttttgc ttgaagtgta
     2581 tatggggatc aaacatgttt caaccaagtg ataaaaccag aataatattt gccattatag
     2641 gggcaagttg catattagtc agtatgtatc ttattccact tattaaaact gagaataaat
     2701 tttgggaaat acttttattt atattaggtg tattgctaat tggaattgcg ttactaagtc
     2761 aaaaaaaata aattttgaaa tagtattagt gagttcgcaa tcatttattg ctagtggttt
     2821 agaactgtta agtttcaaac aataattgat tttaaacctt aggtaaccta taaaaacaaa
     2881 taaggtagag aaaggatatc ttatctaggt agctaagtga taaaaacaca gtcgtctatc
     2941 acttgttagg aaactatcct aactatgctt aataaaaaag gagaaaataa atggaactcg
     3001 tattaccaaa taattatgtt gttcttgagc aagaagagat gatgtatctt gatgggggat
     3061 tttctattcc gagatggcct gttgcaacag ccattaatat agcttttaat ggtgttttag
     3121 gtggaggagc aatcagtcta gttagaaatt atattcgtaa ttatggtttg cggcgagtta
     3181 caagcgcaat tgctggagca gctgcaagat atgttggggt acgagttgca aatagagtgg
     3241 caggatttgc actgtctgct attaatggat ttgcagcttg gatgtcaatt ggcgatgcta
     3301 ttacaacaat ctgggccaac aatgatgtaa ataggagaga cccaaattta aacgccttgt
     3361 ggtaactaga tatgggtatt aaaatggaaa aaatttttgt tattattttt tttgtatgtc
     3421 tatttatatc atccataact tttttagcct atgattttgt tagcgaagaa ataaaaaagt
     3481 tgattatttg gattaatgtc gtttttttga ttttaatcat agcaatgatg atttatccaa
     3541 aattaagaaa atgataagga cactgtaaaa ttatgatacg taaactaatt gttttcttac
     3601 taatgctccc tctttttgtc ttgtggctag agttgcatgt cctagtgaat aacttactat
     3661 tgaatctaga atctcccctt gattttgtga ttagtatgag tcttgctttc tgtagtttga
     3721 ttttatcaaa aatagtttta gacatactat acgcattgaa agatttatac aagaaagaag
     3781 gtcttattaa gatttttccc tttattttta taagaaggaa aaaagtgaat gtgagattct
     3841 cgccctattt ttcattccat cgtaaaagtt tgtcgcctga tgatttaaga tctaggatta
     3901 tatggagttt tattcttgaa attgctatta tcctagtatt tattttaaaa attccttttg
     3961 caatcatcat gcttactaca atatttttct ggactataat ggatatcaat catctcgttt
     4021 ttaataagac ggaatttctt tttaatcaaa acaaatggca aaaagaagat tcatttgaaa
     4081 gtaatttgca taagatattg atggacaaaa tacaaaaatc agaattaagc tattctgact
     4141 taatgtccct tcttctctat gatgcgatga accaatccac ctttttaacg gatagtgaac
     4201 tttttgagga tattttgaaa aaaatcgaag attctcataa cacccttctg tgtacaggtc
     4261 ttgtcgagtt actgctgtat gagatttcta ttagtaacaa taacaactgg caagaaaagg
     4321 ttgataaaat tagaatacag ctcatcagaa tcaatcaatt agattttttc tattatacaa
     4381 gttggttaag gcaaaatttt gatttctgta tgaatagaga gtatcataaa atgaaatctc
     4441 ggaaattatt actgagtaat aaaaagatag tttaagttcg tcctatttgt aaaacaaatg
     4501 aaacaatttc aactaaggag gagaaaacaa atggaacttg tattaccaaa taattatgtt
     4561 gtgattgatg aagaagtaat gatgtatctt gatgggggag cttatttaag caagcgtgct
     4621 tgtcaaggaa tttgcgcagc agcaggtgta ttggctacag cagcagggaa aatagcttac
     4681 tgtattggat atggtgttct taatagaggt tgtgatatta acgagaaccc ttatccttgg
     4741 gatggattca tatctgcaac agtaagatga gtaatgtaga taagattcga aaaatacaca
     4801 tcatcgtgtg ttgggtgtat atatttttat cattcagggc gattataaat gatacggagt
     4861 attttttgct tattttttta gcttttatct actcaatagt atcactacct ctatattctg
     4921 tgaaaaataa aatagtatct atctgtctag ttataaattc gattctgtta atgagtttcc
     4981 ccattttaat caataagttt ttcccagaaa gttttttgac ttataccgta ttaataagtg
     5041 tttttatcct agagttagca atctttcatt taattaggaa agattttgat attaaattga
     5101 ctaacgaata taagaaaatt agtcagttta gaagcaaggt gtctcaatct ccttggataa
     5161 aatatttaga gatttctagt tttatattaa ctatatttcc atctattctt tatggtaaag
     5221 ttgataatca tgtactgact cttatctttt tgataaaaat ttgtgtagat actacgataa
     5281 aatttttatt catcagatta tttgacacaa gtactttaat gaagaggaga atattttttc
     5341 ttttcgcatt ggatgttata gcttacttat ttttaggata tcttttagtg attcaaaaag
     5401 caggctattt gttttcggtt ttacttcttt tttctaattt ttcagttcca tttatcaaag
     5461 aaaaggaata cgaattattt aaaaatagca agtaatagaa atatggatta aatcttagaa
     5521 cggcttaaaa tcagatatga taaagggaaa tagtcttatc taggtaacta agtgctacaa
     5581 acacaatcga ggatcacttg ttaggattac attttaatca tgcttaataa gaatggagaa
     5641 ttcctatggg gattgtatta ctctgaagtt tcgaatacct tagttctcgt gattttgagc
     5701 tattctagct ttagaatcct tcagaacgta aaattttaag cagtcaaaga cttagaatag
     5761 cctgaaaaca tttattttat agcaattcta ggcttagaat tgctcatata tatgtatttg
     5821 agctgctcga tagttaggac agctctattc tgagaaatct gagctatcaa tgcttcagaa
     5881 tagtatttcg ccaatggttt ttattgaaga ggtgattgtg ttatgatagt ctaaaataaa
     5941 attgatttaa tagagtaaat ggagaatcgt tacatgaaac atttttttgc aggaattggt
     6001 gagataaaac ttgttggcta cttattgtat atatgtgtag gactagcccc tctttttcat
     6061 atatatgtca taggttcgga actgagtttt gtgaaaatag tgttatctat tttgggagtt
     6121 atatttgtaa gtatgttaac tattgctcga atttatcgga cttttctata aagaatagtt
     6181 tggacttaaa ctgtatctaa aaacagaggt tggtcgatta tcagcctctt tcttgtcgtt
     6241 gaaaagagtg agatatattg acttttgaaa aaaagatgtc ataattgagt tgggataaac
     6301 ctaaaagtaa tatagtagat tgaatctgga atagtccgtt atgactgtta aaatatttct
     6361 agaaattaat ttgactttcc taatcgattt gttcacatct tgtttcaatc tactatactt
     6421 tctcgttaaa ccgttcggca attcatggtg acatggacac atagtagtaa gcacactatg
     6481 tggcttggca gagctaaaaa ctgttccctt gcgataagcc taataagcac aacataggga
     6541 gttagagaag ccgactctaa tcaaccactt tgggcaatag tgagaactgc cccgtgcttt
     6601 tttattttga gaaaatatgg agtttgtcgt tgaaattact tgattgtatt ttggactatc
     6661 aagaaagatt caatggaaaa acatgtcaag tatcaacgaa ttataagtat ttagagattt
     6721 tcaaagtgaa tttttgtttg actgatttac atcatttatt tgacttatac aaaatcacac
     6781 gagattatgc tagtcaaaca aaacctgcta ttcaagatgg tgtttttatt ttagaagatt
     6841 ttagaaatat cctatgtaca atgatgtaat cgaaaggata tctttatgtg agtttatcgg
     6901 cgatatcttc tatatctaag ctagcaagtt gttgcatcgt agctaaggat ttatctaaaa
     6961 atactatgaa attggacgtc atattttttg aggataaaaa taaaagatcc gcagttttag
     7021 gtttacgaag agacaaaagc ggagtattta aaccagttac tctacatttt acaagcgcta
     7081 agaaatatgc taaagttcgt aaaacagatg tgaaagaaat gaaatggcta taaagattgt
     7141 ccgcaggctt aagcttgcgt tttttgttgt ttaacgaaat taaaaaacaa gctctctcat
     7201 cggaaatgtt tttataggaa tgaaggaaga tgatgctatg tttaaaaaga gaatcgaaaa
     7261 gggcaaaagt agtgtcttta tctttttgga gtagaagctt atgatataga aatttgtaat
     7321 aaaaatgtaa tataaaacat ttgacaaata tttacggggg gagtatactt aatataagta
     7381 tagtctaaaa atgactatca gaaaagaggt aaatttagat gaataagaaa aaaatgattt
     7441 taacaagtct agccagcgtc gctatcttag gggctggttt tgttacgtct caacctactt
     7501 ttgtaagagc agaagaagct cccgtagcta gtcagtctaa agctgagaaa gactatgatg
     7561 cagcagtgaa aaaatatgaa gctgctaaga aggaatatga ggacggaaaa gctgctcaga
     7621 aaaagtatga agatgatcag aagaaaactg aggagaaagc ggaagaagaa agaaaagctt
     7681 ctgaagaaga acaagctgca aatctgaaat atcaacaaga gttggttaaa tatatacgtg
     7741 aaaatgatcc aacaaaaaaa gctgaagcta agaaagcaat ggatgaggct gagaaagagt
     7801 ataagaaaaa acaaacggaa tttgctgaag ttcgtgcaaa ggtaattcct agcgcggaag
     7861 aattaaaaaa gactagacaa aaagcagaag aggctaaagc aaaagaagca gaacttacta
     7921 aaaaagtaga agaagctgag aaaaaagtta ctgaagccaa acaaaaagtg gatgcagaac
     7981 atgctgaaga agtcgctcct caagctaaaa tcgctgaatt ggaacatgaa gttcagaaac
     8041 tagaaaaagc tctcaaagag attgatgagt ctgattcaga agattatgtt aaagaaggtc
     8101 tccgtgctcc tcttcaattt gaattggatg ttaagcaagc taaactatca aaacttgaag
     8161 agttgagtga taagattgat gagttagacg ctgaaattgc aaaacttgaa aaagatgtag
     8221 aagatttcaa aaactcagac ggtgagcaag ctggacaata ccttgctgca gctgaagaag
     8281 acttagttgc taaaaaagct gaattagaaa aaactgaagc tgaccttaag aaagcagtta
     8341 atgagccaga aaaaccagct gaagaaactc cagctccagc accaaaacca gagcaaccag
     8401 ctgaacaacc aaaaccagcg ccggctcctc aaccagaaaa accagctgaa gagcctgaga
     8461 atccagctcc agcaccacaa ccagagaagt cagcagatca acaagctgaa gaagactatg
     8521 ctcgtagatc agaagaagaa tataatcgct tgacccaaca gcaaccgcca aaagcagaaa
     8581 aaccagctcc tgcaccac
//
LOCUS       DAVBHS010000091         8415 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVBHS010000091 DAVBHS010000000
VERSION     DAVBHS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8415
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.091"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            71..1348
                     /locus_tag="VQ651_001461"
     CDS             71..1348
                     /locus_tag="VQ651_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2754438.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITSYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            1361..2008
                     /gene="vex2"
                     /locus_tag="VQ651_001462"
     CDS             1361..2008
                     /gene="vex2"
                     /locus_tag="VQ651_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HET2754439.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            2060..3439
                     /gene="vex3"
                     /locus_tag="VQ651_001463"
     CDS             2060..3439
                     /gene="vex3"
                     /locus_tag="VQ651_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HET2754440.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            3619..3702
                     /gene="pep27"
                     /locus_tag="VQ651_001464"
     CDS             3619..3702
                     /gene="pep27"
                     /locus_tag="VQ651_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HET2754441.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            3751..4407
                     /gene="vncR"
                     /locus_tag="VQ651_001465"
     CDS             3751..4407
                     /gene="vncR"
                     /locus_tag="VQ651_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HET2754442.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASIAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            4404..5732
                     /gene="vncS"
                     /locus_tag="VQ651_001466"
     CDS             4404..5732
                     /gene="vncS"
                     /locus_tag="VQ651_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HET2754443.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGATMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            5873..6754
                     /locus_tag="VQ651_001467"
     CDS             5873..6754
                     /locus_tag="VQ651_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HET2754444.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(7075..8265)
                     /locus_tag="VQ651_001468"
     CDS             complement(7075..8265)
                     /locus_tag="VQ651_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HET2754445.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
BASE COUNT         2595 a         1594 c         1834 g         2392 t
ORIGIN      
        1 ttttttttcg tatttcataa acatttcata tttgggtttt ataatagtct tacaaatatg
       61 gaggtgacaa atgaatccaa tccaaagatc ttgggcttat gtcagcagaa agcgactgag
      121 aagttttatt ttatttctga ttttattggt cttattggcc ggaatttcag cctgtttgac
      181 tctgatgaag tccaacaaaa cagtagaaag caatctttat aaatcactca atacatcttt
      241 ttctattaag aagatagaga atggtcagac attcaagttg tcagacctag catctgtaag
      301 caagattaag gggctggaaa atgtctctcc tgaacttgag acggtcgcaa aactaaaaga
      361 caaggaagca gtgactggtg agcagagcgt ggagcgtgat gatttatcag ctgcagacaa
      421 taacttggtt agcttaacgg ctcttgagga ttcatccaag gatgtaacct ttaccagttc
      481 ggctttcaat ctaaaagaag ggcgacacct tcaaaaaggg gattccaaga aaatccttat
      541 ccacgaagaa ttggctaaga agaacggtct ttcacttcat gacaagattg gcttggatgc
      601 tggtcagtct gaatctggaa aaggacaaac agtagagttt gagattatcg gcatcttttc
      661 tggtaaaaaa caagagaaat tcacaggctt gtcttctgac ttcagtgaaa atcaagtctt
      721 tacagactat gaaagtagcc aaaccctttt gggcaatagt gaagctcaag tcagtgcagc
      781 acgcttctat gtagaaaatc ctaaggaaat ggacggactc atgaagcagg tagaaaactt
      841 ggccttggaa aatcaaggct accaagtcga aaaggaaaac aaggcttttg aacaaatcaa
      901 agactcagtt gcaactttcc aaaccttctt gaccatcttc ctttatggga tgttgatagc
      961 aggagctgga gccttaattc tggttttgtc tctctggttg agagaacggg tctatgaagt
     1021 ggggatttta cttgcacttg gaaaaggcaa gagctcgatc ttcctacaat tctgtttaga
     1081 ggtagttttg gtatctcttg gagctttgct tccagcattt gttgcaggaa acggaatcac
     1141 aagttatcta ctccaaactc tactagcaag tggagatcag gcaagcttac aagatacact
     1201 agccaaagca agcagtttat caactagcat cttatctttt gcagaatcct atgtttttct
     1261 agttctgctt agttgcttat ctgtagccct ttgtttccta ttcttattta gaaaatcacc
     1321 gaaagaaatt ttatcatcta ttagttaaga aggagaaatc atgactttat tacaattaca
     1381 agatgttacc taccgttata agaatactgc tgaagcagtc ctatatcaga tcgattataa
     1441 ttttgaacct ggaaaatttt acagtattat tggggagtca ggagcaggaa aatccacact
     1501 cttgtcccta cttgctggtc tagatagtcc tgttgaaggt tctatccttt ttcaaggaga
     1561 ggatattcgt aagaagggct attcttacca tcgcatgcac catatttccc tggtctttca
     1621 aaattataac ttgatagatt atctttctcc gctggaaaat atccgcttgg tcaacaaaaa
     1681 ggcaagcaag gatacacttc ttgagcttgg tttggatgaa agccagatca agcggaatgt
     1741 tctccagtta tcaggtggtc aacagcaacg tgttgccatt gctcgcagtt tggtctcaga
     1801 agctccagtt attctagctg atgagccaac aggaaatctg gatcctaaaa ctgctggaga
     1861 tattgtcgaa ctactcaaat cacttgccca gaaaacaggt aaatgtgtga ttgtcgtaac
     1921 tcacagtaaa gaagtggcac aagcgtcaga tattacactt gaattaaagg ataagaaact
     1981 gactgaaacg cgcaatacta gtaaataatt tgagcttatt ttgatagaat gattaaaaca
     2041 aaatctagaa agggaatcta tgttacacaa cgcatttgcc tatgttacaa ggaagttttt
     2101 caaatcgatt gtcatcttcc tgattattct cctcatggcg agcttgagtt tggtcggctt
     2161 gtcaatcaag ggagctactg ccaaggcttc tcaggagacc tttaaaaata tcactaatag
     2221 cttctccatg caaatcaatc gtcgcgtcaa ccaaggaacg cctcgtggtg ctgggaatat
     2281 caagggtgaa gacatcaaaa aaatcaccga aaacaaggcc atcgagtctt atgtcaaacg
     2341 tatcaacgct atcggagatt tgactggata tgacctgatt gaaacgccag aaaccaagaa
     2401 gaatctcact gctgatcgtg ccaagcgttt tggaagtagc ttgatgatta caggtgtcaa
     2461 tgactcctct aaagaagaca agtttgtctc tggttcttat aaactagtcg aaggagagca
     2521 cttaaccaac gacgacaagg ataaaatcct cttgcacaag gacttggcag ccaaacacgg
     2581 ctggaaagta ggggacaagg ttaaactgga ctctaatatc tacgatgcag ataatgaaaa
     2641 aggagccaag gaaacagttg aagtgacaat caagggactc tttgatggtc ataataagtc
     2701 agcagtaacc tactcacaag aactttacga aaacacagct attacagaca ttcacactgc
     2761 tgcaaaactt tatggataca cagaagacac agccatttat ggggacgcaa ccttctttgt
     2821 aacagcagac aagaacttgg atgatgttat gaaagagttg aatggcatca gtggtatcaa
     2881 ctggaagagc tacacactcg tcaagagctc ctctaactac ccagctcttg agcaatctat
     2941 ctctggtatg tacaagatgg ccaacctcct cttctggggt agcttgagct tctcagttct
     3001 cctccttgcc ctcttgctca gcctttggat caacgcccgt cgcaaggaag tgggaattct
     3061 cctctctatc ggcctcaagc aggcaagtat cttgggtcaa ttcatcaccg aatctatctt
     3121 gattgctatc cctgctctag tttctgctta cttcctagct aattacactg cccgtgcaat
     3181 tggaaacact gtccttgcca atgtgacttc aggtgttgcc aaacaggcta gtaaggcggc
     3241 tcaagcctct aaccttggtg gtggtgcaga agtagatggc tttagcaaga ccttgtcgag
     3301 cctagacatt tccattcaga catcagactt tatcatcatt tttgtccttg ccttggttct
     3361 agtggttctc gttatggcgc ttgcttcaag caatctcctt agaaaacaac caaaagagct
     3421 cttgctggat ggtgaataaa tttgaaaaaa tgagtctaga ataaagattg catcttgtgt
     3481 ttctattcaa gaatagtgga taggaatggc tatttaacaa ttcaaaataa atccgaaagc
     3541 agtggtgaaa atcattgctt tcagttgctt tctttgtact ttagtgctta aatataatat
     3601 actaaagtta tggaatttat gagaaaggaa tttcacaacg ttttatctag tggtcagttg
     3661 cttgcagaca aaaggccagc aagagactat aatagaaaat agagtaggta tttattctaa
     3721 gaaaaataaa aaatagagag cagttaaagt atgaaaattt taattgtaga agatgaagag
     3781 atgatccgtg agggggtcag tgattatttg acggattgtg gctatgaaac tattgaggca
     3841 gcggacggtc aggaagctct ggagcaattt tctagctatg aggtggccct ggttttactg
     3901 gatatccaga tgcccaagct caacggctta gaagtcctag ctgagattcg taaaaccagt
     3961 caggttcctg tcttgatgtt gacagctttt caagatgagg aatacaagat gagtgccttt
     4021 gcctctttgg cagatggcta tctggaaaaa cctttctccc tctccctttt aaaagtgagg
     4081 gtggacgcga ttttcaagcg ctactacgat acaggacgaa tcttttctta caaggatacc
     4141 aaggtggact ttgaaagcta cagtgcaagc atcgcaggtc aagaagtgcc tatcaatgcc
     4201 aaagagttgg aaattctgga ctatctagtg aaaaatgaag gccgggcctt gactcgatct
     4261 cagattatcg atgccgtctg gaaagcgaca gatgaggttc cctttgaccg tgttattgat
     4321 gtttatatca aggaattgcg gaaaaagcta gacttggatt gtatcctcac tgtgcgcaat
     4381 gttggttata aattggagcg aaaatgaaac gaacaggttt atttgcaaag atatttatct
     4441 ataccttctc gatatttagt gttctggtta tctgccttca tttagctatt tattttcttt
     4501 ttccttcgac ttatctgagt catcgtcagg aaaccattgg tcaaaaggca acagccattg
     4561 cccagtccct agaagggaaa gataggcaga gtatcgagca agtgttagac ttgtattccc
     4621 agactagtga tatcaagggg accgtcaaag gtgagatgac cgaggacaag ttagaagtca
     4681 aggacagtct tcctctggac acagaccgcc agacaacctc tctctttatt gaggagcgcg
     4741 aggtgaaaac gcaagacggt gctactatga ttctccagtt tctagcttcc atggatttac
     4801 aaaaggaagc ggagcaaatc agtctccagt ttcttcccta taccttgctg gcctcctttc
     4861 tgatttccct cttggtggcc tacatctacg ctcggactat tgttgcaccg atcttggaaa
     4921 tcaagcgggt gacccgtcgg atgatggacc tggattccca agtgcgattg cgcgtggatt
     4981 ctaaggatga gataggtaat ctcaaggaac aaatcaatag cctctaccag catctcttga
     5041 ctgttattgc ggacttgcat gaaaagaatg aagccattct ccagctggag aagatgaagg
     5101 tcgaattcct acgaggagct tctcatgaat tgaaaacacc gctggctagt ttgaaaatcc
     5161 taatcgaaaa tatgagagag aatatcggtc gttataagga tagagaccag tatctgggag
     5221 ttgccttggg gattgtggat gaactcaatc accatgttct gcagatactt tccctctctt
     5281 ctgtgcagga attgcgagat gatagggaaa caattgacct cctccagatg acgcaaaatc
     5341 tggtcaaaga ttatgccttg ctagccaagg aaagagagct ccagatagac aatagtttga
     5401 cccatcagca ggcttatcta aacccatcag ttatgaagtt gattctttct aatctcatca
     5461 gcaatgccat taagcactct gttccaggtg gcttagttcg aattggagaa agagaaggag
     5521 aactttttat cgaaaatagc tgtagctcag aggaacaaga aaaactagcc cagtcttttt
     5581 ctgacaatgc cagtcgcaag gtcaaggggt ctggtatggg gctctttgtg gttaagagtc
     5641 tattagaaca tgaaaaatta gcttatcgtt tcgagatgga ggagaatcgt ttaaccttct
     5701 ttatagattt tccaaaagtc gtccaagact agggagagaa agggtttaca tagatggagt
     5761 tagaagaaaa tcaatcgaaa ctgcgggaaa aactagattt ttttggcaaa aagtgataaa
     5821 atgaacaatg taaatgggat gacccataaa aatatacagg aggcctgata aaatggcaat
     5881 cgtttcagca gaaaaatttg tccaagcagc ccgtgacaac ggttatgcag ttggtggatt
     5941 taacacaaac aaccttgagt ggactcaagc tatcttgcgc gcagcagaag ctaaaaaagc
     6001 tccagttttg atccaaactt caatgggtgc tgctaaatac atgggtggtt acaaagttgc
     6061 tcgcaacttg atcgctaacc ttgttgaatc aatgggtatc actgtaccag tagctatcca
     6121 ccttgaccac ggtcactacg aagatgcact tgagtgtatc gaagttggtt atacttcaat
     6181 catgtttgac ggttcacacc ttccagttga agaaaacctt aaattggcta aagaagttgt
     6241 tgaaaaagca cacgctaaag gtatctcagt agaagctgaa gttggtacta tcggtggtga
     6301 agaagacgga atcatcggta aaggtgaatt ggctccaatc gaagacgcta aagcaatggt
     6361 tgaaactggt atcgacttct tggcagctgg tatcggtaac atccacggcc cttacccagt
     6421 aaactgggaa ggtcttgacc ttgaccactt gcaaaaattg acagaagctc ttccaggatt
     6481 cccaatcgta ttgcacggtg gatcaggtat tcctgatgag caaatccaag cagctatcaa
     6541 acttggtgtt gccaaagtta acgttaacac agaatgccaa atcgcattcg ctaacgcaac
     6601 tcgtaaattt gctcgtgact acgaagcaaa cgaagcagaa tacgacaaga aaaaactctt
     6661 cgacccacgt aaattcttgg ctgacggtgt aaaagctatc caagcatcgg ttgaagaacg
     6721 tatcgacgta ttcggttcag aaggtaaagc ataatctagc tgaataatac agataaaacc
     6781 tgcctgtgag ggtgggtttt ttgatttcaa gctactcgtt ttagtatagt aaactgaatc
     6841 tagactagca caatgctgct tctatagcgt tttgaaacta gaataggaca acataactgc
     6901 taaaagattt ctataaattc atttgatttt cctaatcaat ttgttcgtat cctatttcac
     6961 tccactataa aatattctta tcaattgatt tgaatgccaa aattccatcg ttccggtttt
     7021 atttcatttg gctataaaaa actctcctgg gaaatcccag gagagtatga ttacttattt
     7081 gaacttgaat ccttcgtaaa taagctctgt ttttggattt tgtttcttaa tctgtttggc
     7141 aagtgccttc atcatagaaa taggaccaca catatagacg gttgcatgtt cgggcacttc
     7201 tttttgttca aaattaagat agccgtcttt cgtactgtcg attagatgga gttcaaaatt
     7261 aggatttttc tgagcatagt tacggagtaa atctaggtag actgcatttt catctccacg
     7321 gaagctatag tagaagtgaa cctgtttatc taaaatagga tgttcacgga tgtaagagat
     7381 gaagggggtg atcccaatac ctccagcaat ccaaacctga ttttctcgtc cttcttctat
     7441 gatcatgtgt ccgtaagctc tgtctagggt tactttgctg ccggcttgaa gattatcata
     7501 gatattattg gtatggtcgc ctgaattttt aacagtaaag taaagagttt gaccatgacc
     7561 tcctgagata gaaaagggat gcggagcact ttcaaagcct tcttggaaaa tctttagaaa
     7621 ggcaaattgt cctgattgat agttgaaagg tctgctaaga tggatttgaa tttctctagt
     7681 atcgtgattt aagcgtttga gatgggtaat ttttcctaga taggggaagg aaatcttttg
     7741 atatagaaaa atgatataaa aaccagctag taagcctaaa agggcatagc taccaacaag
     7801 aaaacttaga agattaaatg taaggagacg attgcccatt atcatgtaga tgtgaaagag
     7861 tcctaaaata taggctaggt aaaccaggcg gtgaatccat cgccaagctt cgtattggat
     7921 gtatttgcct aaataggcga caaggatgat gctggcaaag atatagatgg caagattacc
     7981 aaactgagca gctaagcgag agccccacaa accgcccata ctaaagttat gaaagattag
     8041 taggatgatg gagagaaagg ctgtgaattt gtggacggtg tagaccttct ccaaaccgtg
     8101 aaaccagctt tctagtagtg ggagacgagt ggctaggata aaagtcagag ataggcttgt
     8161 taaagctagt cctggaatca tgaattgggg agaagtgttc atccaagtca aaagagtcaa
     8221 gataaaacta gctatgataa agagtagtcc tttgactgat ttcatagaaa attccatttc
     8281 atttagattt cgatttgttg taaatgaatt tgttacattt tatcatagaa aatgtatggt
     8341 gtcaaattga ggtctataaa tatctactct catcaaaaaa ctctccaatt gaactggaga
     8401 gtggctgttt atact
//
LOCUS       DAVBHS010000092         8359 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVBHS010000092 DAVBHS010000000
VERSION     DAVBHS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.092"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(616..924)
                     /locus_tag="VQ651_001469"
     CDS             complement(616..924)
                     /locus_tag="VQ651_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2754446.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
     gene            complement(921..1229)
                     /locus_tag="VQ651_001470"
     CDS             complement(921..1229)
                     /locus_tag="VQ651_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2754447.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            complement(1234..2529)
                     /locus_tag="VQ651_001471"
     CDS             complement(1234..2529)
                     /locus_tag="VQ651_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2754448.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTKFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPSAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGIVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            complement(2547..3956)
                     /locus_tag="VQ651_001472"
     CDS             complement(2547..3956)
                     /locus_tag="VQ651_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET2754449.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTEDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSLCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            4114..4842
                     /locus_tag="VQ651_001473"
     CDS             4114..4842
                     /locus_tag="VQ651_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2754450.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            5302..5496
                     /locus_tag="VQ651_001474"
     CDS             5302..5496
                     /locus_tag="VQ651_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2754451.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSDVTLITGKNGTGKSTLL"
     gene            complement(5862..7172)
                     /locus_tag="VQ651_001475"
     CDS             complement(5862..7172)
                     /locus_tag="VQ651_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET2754452.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNFAITNGTLDVLAQHILYRTRTYPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTSIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            7409..8281
                     /locus_tag="VQ651_001476"
     CDS             7409..8281
                     /locus_tag="VQ651_001476"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET2754453.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT         2603 a         1471 c         1583 g         2702 t
ORIGIN      
        1 ttcacatctt atttcaattc actatagttt aatttaagag tagtatttac taaggcccaa
       61 ttaaaatcaa agagcaaact agaaaacgag tgccattcag ctcaaaacac tgatttgaga
      121 ttgcagataa gactagcccc tcattaacag gtttacgata aaacgatgac aaggtgtgtt
      181 gctttttgat ttctaaagag tataatgata gatctctata aaataagtgc gaaggaaatg
      241 agcttttata gtcctttcgt tttaaaatac tatctcagat gttcttatat cgacaagaag
      301 tttttgagtc attccctcat catacatatt aaataaatag tggctcattc aattttttac
      361 tagaataata agctagtata gtaaactgaa ataagatata aacaaataaa ttggagctta
      421 acatctattt ccagcaattt tttagaaact gcagtggact attctaaatt caacacatta
      481 taaaaactag agtaaaagaa aaggattgga tcttgtgtaa tgcaggatct aatcctttca
      541 atcattttgt ccaacttttg gaggttccta caatgtagcc gtcattaata aagacagatg
      601 ggaacgacag tgttcctatt tattttgata gagatcgatg aattctttag atagcaactg
      661 aataatctct gttgaagcca tttggtcttc tgcatgcata aatagcaagg agaatcctat
      721 tttttctcca gtagcttctt tttgtatgag attagagtga atcttgtgcg cttctactaa
      781 ggagtcttcc gcttcttcaa ctttaatttt cgcttctttt aaatttcctg ccttagctag
      841 ttggatggct tcaataaagg atgatttggc tgctccacta ttggcaatga gctgaaaaca
      901 gatatattcc atttcttctg tcatcttatt tctcctatcc atgcaagtgc ttgttccaga
      961 acttttgctc cattcatcat tccgtaatcc cgcatatcaa tggtatctac agggatattt
     1021 cctgcaattt ctttcacagc aagtaactca taacgaattt gtggcccaat tagaatgaca
     1081 tctgcttcat ggatattctt tttcgcttct gtcattgatt ttgcttggat agaaatttca
     1141 atcccacgtt cagtcgcact ttgttgcatt tttttaacaa gcatacttgt cgacattccc
     1201 gcattacata ctaataaaat ttgtttcata atcttaacct tccatttctt gctcaacaac
     1261 tttgtcatta actttgataa atggaatgta tagaagaact ccaagtgcaa agatgatgaa
     1321 ttgaactaga actgctctca cgtcccctgc tgttgctaac catgcattta agaatactgg
     1381 tgtagtccaa ggaacttgta taaatgcagg actcatgaat cctgtaactg ttgctaagta
     1441 gctgattaaa ataccaagga ctggaactgt gataaatgga atagctaatg aaatgttata
     1501 aacgattggg taaccgaata atactggttc attgatattg aagataccag gtccaaaaga
     1561 taatttagcc acgtttttag aaacagcatt gcgactcact aagaatgttg ctattaataa
     1621 acataatgta gatccactac cacccattaa agcgaatgtt tgtatttgtg ataagttgat
     1681 gatgtgtgga atggcttgtc cattatttgc tgcagtgatg ttttcagtaa tgttaattaa
     1741 tagtaatggt tctaggatgg cactgtaaat aactgcttgg tgaataccaa atagccataa
     1801 catatttcct aaagagtaaa taataatgac cccgattaag cttgtaccaa tatgacgaat
     1861 tggttcttga ataaagattg taatgattga gattaagttc attccagtta tattgaataa
     1921 taatgctgaa acaatcccaa ataaggagat gacagtcatg actggaagta atacgctaaa
     1981 tgatctacta acagctggtg gaatattttc accaaggttc atttgtaaag ctttaacgtt
     2041 tgataattca atgaataatt ctgttgcaat aatcgctacg ataaccccgg cgaacattgc
     2101 gcctgtacct gtgttgttga atgaaagaac acctgaaatg tttacagcat cttttgctcc
     2161 gtcaggaact acagaaactg tatttggcat catcacaatt aaagaaacta atgatagcat
     2221 tgatgctgct gacgggtttt cgaaatctct gtttttagct aagaaataac caaccattac
     2281 agcaataatc atacctgaaa tacttaaagt accgtttgca attgttattc cccaatattg
     2341 gaattttgtt aatgtatccc cttggaaaat ccacttaaat accgtgttgt tcaaaagaac
     2401 gattaaacct gccaaaatat ataatggcat tactgttacg aatgcatctc ttagggtttt
     2461 taaatgaatt tggttcccta gtttaccagc aaaggtggca aattgatcta taaattttga
     2521 gtttttcatc tgaatatctc ctctctttac tcctcaattt ctacttcaaa accgttattg
     2581 tcacttactt tgcgatacca tcttcctgag ctcttgatgg ttctcttttg cgtttctaaa
     2641 tcaacggaga taaatccgta acgattctta tatgcattat tccaagacca gcaatcaaat
     2701 gcggtccaag cgtggtatcc aaaacagaga cttccctctt caatggcttt atgtagccat
     2761 cttaaatgtt cttcataaaa ttcaatacga tacacgtcat cgataactcc attttcatcg
     2821 ataaaccgtg cttcgttttc aacacccatt ccgttctcac tgataaacca tgggatatta
     2881 ccatattctt ccttcacaat catagcaata tcataaatgg ctttcggaaa aatttcccaa
     2941 ccacgatatg gattcatgcg acgctccggc cattcatact ctttgaagta ttggtctggc
     3001 atccagggcg tctgatattc ctcaggattt gcttgtgctt gaacacgttt tggatggtag
     3061 tagtttactc caagaaaatc aaccgtattt gatttcatca gttgaagctc tttttcggta
     3121 tgactccata acacgccatc tctctctagc tgttttacca atctttctgg gaaagttcct
     3181 ttaacagctg gattcaagaa gactttgtta aagaagtctt ctgtaaatcg acttgcttct
     3241 aagtcttctg gagaattact tcttggataa gcaggtgtca agtttaaaat aatcccaatc
     3301 tttccatcaa gtcctaatga gcgatatagt tgaatcactt ttgcactagc aaggtttaga
     3361 ttatagatga cttgtacggc ctcttttccc tttcctttta gatttggata atggaaagca
     3421 tataagtatc ctgcttctgg aatgaccatt ggctcattga aagttgtcca gtaatgaacc
     3481 ttatctccaa agcatgtgaa agcagtcttg gcaaacttca cgaataactc cactacatgt
     3541 ttgctttccc aaccaccgta tttttgaaga agttccactg gtaaatcaaa atgatgtaaa
     3601 ttcatcacaa gatccatctg gttcttttta gcttcttcaa tgatggcatt gtagaaagca
     3661 ataccttttg gatcaggctc acctgtctct aaattcttga tgagtcgact ccattggatg
     3721 gaagtgcgga aagaattaac tccaatttcc ttcatcaagt ggaaatcttc ggtgtaagta
     3781 tgaaaaaagt tactggctac aagaggtccg acattatcga aaaaatcttc tggatgcgtt
     3841 ttgaaccagt agtccatcac attttcatgt acttttccat attgaccttc tgtctgaggt
     3901 ccgctagaag cagctcccca ccaaaacaga ttaggaaata ctaacttttt tttcatgctc
     3961 aacctccttt ctattatgct atacataaag tatagcataa ttttaaaatt tgtctagaca
     4021 tttttaaaaa ttgttgatac atatattgtg tttgcctcat ttcttgaact ttgttatact
     4081 actattagaa tattatagag agaaccagat taaatgagta caaaatattt atttatttac
     4141 aatgagattc gtgaaaagat tctctgtaat aaatatacca tgaacgaaca attgcctgat
     4201 gaaatgacat tagctaaaca gtttgcctgt agtcgaatga cgatcaaaaa agctttagac
     4261 ttgttagttt ctgagggctt aatttttaga aaacgtgggc agggaacctt tgttctctct
     4321 cgtggcagct caaaaagaaa attaatcgtt ccagaaagag atatccgggg actgacaaaa
     4381 atatctgaag atgctcattc tacaattgac tcgaggatta ttcacttcaa attagaattt
     4441 gcaaatgaat ttttagcaga aaaactacag gtcgctttgc agagtccagt ttataatatt
     4501 taccgcctgc gtattattga cggtaaacct tatgttctgg aacaaactta tatgagtacc
     4561 gatgttattc caggtattac tgaagatatt ttacaaaaat cgatttacaa ttacattgaa
     4621 ggaaagttag gattgcatat tgccagtgct acaaaaatct tgcgagcttc ttctagttca
     4681 gaaaatgagc aacattactt gcagctcctt ccaacagaac cggtatttga agtagaacaa
     4741 gtggcttatt tggataacgg aactccgttt gagtactcga ttagtcgtca tcgctatgat
     4801 ttatttgaat ttaattcttt tgcattacga cattcctcct aggagaaaat gtgaaaatga
     4861 agccaatctt ttacaggctc tagtttaaga aaaatttaaa acagggcaag aaggtcccat
     4921 ctatgcttaa atggtttctc ttttctaaat aagatggctt taaaagagtg atcgttttat
     4981 ccatcatgtt gaaaaatatc ttcgtatagc ttatagagta ggtactgaaa ttgttcacct
     5041 gatctacttc ttatagttat ttagttttaa atagtgtttc aaacattctt acactgacga
     5101 gaagtttttg agtcttttct tgtaacacat atagtatact gaggttagaa tagtagactg
     5161 tgacttctaa caaattgcta gaaatgaatt tcaatctccc aatttatttg cttatatatt
     5221 attttaatat attaaataaa ttctggatca taatcattta aaaaaatttt atttttttat
     5281 ttttcattac gaataatata gatgaagggg aaagagtatg aaaacagaac tgtttctttt
     5341 gctattagtt caaaaggaga aaaaatgaaa gtagaaaata tttcgtatag ggtggatcat
     5401 cgtatattgt ttgataatat ttcttttgat acttcgagtt cagacgtgac attaattact
     5461 ggtaaaaatg gtacaggaaa gtcaacttta ctatagtgga ttgaaactag aatagtacac
     5521 atctacttct aaaatattgt tagaaatcga tttgactatc ctgatctatt tgtcctgttc
     5581 ttatttcatt tcactatatc tcaaattgag tatgacgaag tgcgctccca tgtcctggga
     5641 acgcactttc ttcatatttt tcatattctt gaatccatcg ataaagacta ttgggatgaa
     5701 tttttaaagt tgaactaatc atttttacag gatgaggttt acagcagaga gtttgaaggc
     5761 tttatcaaag gtttttcttg gcataatgac ttttcctcgt ttccacttaa ttttgtgtct
     5821 actttattat accaagtcca cccttaagtt agataataaa tctaacttaa ggaagctaga
     5881 aggatgagaa tccaggtggt caagagtccc aaacttaagc tgattgggac acccagaata
     5941 atttgctttt tgaaggcaag gccacgttcc tctatattgg gaagtgagag ttgaatgaga
     6001 gaaccagctg atgaaaaggg tgagatatta gtagatagag cgccaataac ggtggctgtt
     6061 gtgagtaagt gaatatcaat ctggggattt tgagcactga tgatagcaat gatgggaaag
     6121 agggctggag ctacaacgga tagggtggaa ctaaagagtg acatcacccc ggctatcaca
     6181 caaaagaaca gaggtaacca gaaatgagga atggatgttg tcatgaggtg ccctatcagt
     6241 gtgactaaac ctgacttgac cgctagagac attagtaagc tcatgccgca gagcatgata
     6301 attgtagccc agggaacttt agccaaaatg gcttcttgct tccctaattt gagccttaag
     6361 gcgaggcaga ccatgagtat tgagacaaag ccaatatcaa atgtttttcg ataagtagct
     6421 atccaggcga tgtttgggaa aatgagatgc aacaagggaa aaagccaaac caaaaccatg
     6481 ctgctgatca tgagcaaggt ggtttgtctt tgaaccttgc tgaggagtgg tggttggtca
     6541 atagtcaagg atgagtttgt tcttccctta ctatagtgac tgtaacagga taataaaagc
     6601 aagacaatga gtgggtagat aatgctgacg ataaagatat gattgccaag tgaaaaagct
     6661 tgctcttccc atcccatttg cttaaacagg ccttgaaaga caatgcctga gctactggtt
     6721 atcaaattag cccctcctga agctccccaa ttgacggctt gagctccaat caaagggtgt
     6781 ttgtccgctt tttgacagag agtaatcgct agaggacagc aaacggccat agtagtgaaa
     6841 aatccagcac ctaaagcaga caaaagggtt gccatcaggt ataaaatcat gtagagggcg
     6901 ttagggtagg tgcgtgtgcg gtagagaatg tgttgagcca aaacatcaag agtaccgtta
     6961 gttattgcaa agttataaaa gagagagacg ctaaaaatgg taaaaaagag tgaggttggc
     7021 caaaaatgaa gaagttcttt ggggcttaat cccatgagag tggttgcgat gaggtaagaa
     7081 aaagcaatag ccagcaggcc aatattgatt ttggtgcggt aaccaattcc aatggctaga
     7141 gcaatggcgc taatcattat taaatgaatc attgaattgt cctttcagtt agaatatgga
     7201 aataggttgg caattttggc gctcaaaaga ccacctgttt cctattggta cttgactatt
     7261 atacacaaaa aaaatacaat tgtcttgaca attgtattga cacttgttag aattaaaaat
     7321 gaggtattca ggcttgtgat ttcctatgga agttaatagt gattgcctct aatgcttaca
     7381 agtgatatta aaaatagagg acctagtgat gtcaatcatt tcaactgatt taaccccttt
     7441 tcaaatagat gatacattga aagcagcctt gcgagaagat gttcattccg aagattacag
     7501 taccaatgcc atttttgatc atcatggcca agccaaggtg tcgctttttg ccaaggaagc
     7561 tggtgtttta gcggggctaa ccgtttttca aagggttttt accctatttg atgccgaggt
     7621 gaccttccag aatcctcatc aatttaagga tggggatcgt ttgactagtg gcgatttggt
     7681 tttagaaatc ataggctcgg tgagaagtct cttaacatgt gaacgcgttg ccttgaattt
     7741 tttacaacat ttatcaggga ttgcttcgat gacagctgct tatgtagaag ccttaggcga
     7801 tgattgcatt aaggtatttg atactcgaaa aactactcct aatttacgtc tttttgagaa
     7861 atatgcagtg agagttggcg gtggctataa tcatcgcttt aatttatcag atgctatcct
     7921 gctaaaagac aatcacattg cggcagtagg tagtgttcaa aaggcaattg ctcaagcgcg
     7981 tgcctatgcc ccttttgtga aaatggtcga ggtggaagtg gaaagccttg ctgctgccga
     8041 agaagctgcg gcggcgggtg ctgatattat catgttggat aatatgtcat tggaacagat
     8101 tgaacaggcc attaccctaa ttgcaggacg ttctcggatt gaatgttctg gaaatattga
     8161 tatgaccact attagccgtt ttcgtggttt agcgattgat tacgtctcca gtggtagttt
     8221 aacccatagt gctaagagtc ttgatttttc catgaagggg ttaacctacc ttgatgtcta
     8281 agttgtaaaa taaactaact ttttaaagga tgtctttcct ctagaacgag ttttatgtca
     8341 gatagtttaa acgcctctt
//
LOCUS       DAVBHS010000093         8175 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVBHS010000093 DAVBHS010000000
VERSION     DAVBHS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.093"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            106..1731
                     /gene="treC"
                     /locus_tag="VQ651_001477"
     CDS             106..1731
                     /gene="treC"
                     /locus_tag="VQ651_001477"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HET2754454.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTGNGIGDFRGIIGKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCIGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            1873..2121
                     /locus_tag="VQ651_001478"
     CDS             1873..2121
                     /locus_tag="VQ651_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HET2754455.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            2328..3122
                     /gene="racE"
                     /locus_tag="VQ651_001479"
     CDS             2328..3122
                     /gene="racE"
                     /locus_tag="VQ651_001479"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET2754456.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIYVEHVEL"
     gene            3119..4090
                     /locus_tag="VQ651_001480"
     CDS             3119..4090
                     /locus_tag="VQ651_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HET2754457.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGKSSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            4066..4587
                     /locus_tag="VQ651_001481"
     CDS             4066..4587
                     /locus_tag="VQ651_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET2754458.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            4584..5045
                     /locus_tag="VQ651_001482"
     CDS             4584..5045
                     /locus_tag="VQ651_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2754459.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            5036..5776
                     /gene="xerD"
                     /locus_tag="VQ651_001483"
     CDS             5036..5776
                     /gene="xerD"
                     /locus_tag="VQ651_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET2754460.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            5776..6504
                     /locus_tag="VQ651_001484"
     CDS             5776..6504
                     /locus_tag="VQ651_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET2754461.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            6523..7092
                     /locus_tag="VQ651_001485"
     CDS             6523..7092
                     /locus_tag="VQ651_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HET2754462.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKSQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            7079..7801
                     /locus_tag="VQ651_001486"
     CDS             7079..7801
                     /locus_tag="VQ651_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2754463.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            7801..8043
                     /gene="yidD"
                     /locus_tag="VQ651_001487"
     CDS             7801..8043
                     /gene="yidD"
                     /locus_tag="VQ651_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET2754464.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
BASE COUNT         2364 a         1631 c         1948 g         2232 t
ORIGIN      
        1 ggctagcttc ctagtttgct ctttgatttt cattgagtat aattgaaata gcacaaatag
       61 ggacggaaga ctgcttcctc ccttttatca gaaaaggaga aacagatgac acttgataaa
      121 ggaaaagtag tctatcaaat ctatccaaaa tcttacaaag acaccactgg caatggtatt
      181 ggggatttcc gtgggattat cggaaaaatt ccctatctag ccaaactagg tgtggatatg
      241 gtctggctca atccattcta tccaagtcct caacgggata atggttacga tatttcagat
      301 tatatggcag tggatcctct ttttggtgat atggctgatt ttgaggaaat ggtgtgtatc
      361 ggtaaagagc acaagattga ctttatgctg gacatggtac ttaatcattg ttcgacagag
      421 catgaatggt ttcagaaagc cctagctggt gataagtatt atcaagactt tttcttcatc
      481 caagaccaac caacagattg gcagtctaag tttggtggtt ctgcatgggc gcctttcggg
      541 gatactggga aatactatct tcacctattt gatgagaccc aggctgacct taactggcgc
      601 aatcccaatg tccgtaagga gcttttcaag gttgttaatt tctggcgcga caagggtgtc
      661 aaaggttttc gatttgatgt gatcaatttg attggtaagg acgaggtttc agtggactgt
      721 cctgaaaatg aagggaagcc agcttataca gacaaaccca tcgttcataa ctatttgcgt
      781 atgatgaacc aagccacttt tggatctgac gatagcttta tgacagttgg ggaaatgtct
      841 tctaccacca tggaaaactg tgtcctctat tcgtcgcctg atcgtcagga attatccatg
      901 acctttaatt ttcatcacct caaggtggac tataaggatg gacaaaagtg gaccttggct
      961 ccctttgatt ttgaagagtt gaagagtctt taccatagtt ggggtaagga aatgagtgat
     1021 aaagacggtt ggagtgccct cttttggaac aatcacgatc aaccacgtgc cttgaaccgt
     1081 tttgtcgata ttcaaaactt ccgcaaggaa ggagccacca tgctagcagc cagcattcac
     1141 ctgtcacgtg ggacacctta tatctacatg ggtgaggaaa tcgggatgat tgacccagac
     1201 tatgattcta tggctgatta cgtggatgtc gaatcgctta atgcctatca gatgctccta
     1261 gaagaaggaa agagtcagca agaagccttc cagattattc aagctaagtc gcgtgataat
     1321 tcacgcattc ccatgcagtg ggatgcttcg gaaaatgcag gattttcaac aggcactcct
     1381 tggttgaaag ctggtaaatc ctataaagac atcaatgtag aaaatgaaat ccagggtccg
     1441 atttttacct tctatcaaga cttgattcga ctgcgtaagg aaatgccaat catttcagaa
     1501 ggaagttaca aaccagcctt tgaagatagc aaacaagtct acgcttttga acgccagttt
     1561 gaggatcaaa agttactagt gctcaataac ttttatgcta aagaagtgga aatcgacttg
     1621 ccagcagtct accaaaatgg acagattttg atttcaaact atgaagatgc agaagtgtct
     1681 gagaaaattc tactaaaacc ctatcaaaca ctggctattt atgtaaatta aacttagcaa
     1741 ggagacctct ttttcagaaa agcagggttc tttttgctgt ctattccatc cctgaaagtg
     1801 tagaacaaac tggtcagggt ggctagtttg tggtataatg aaagggactc aaaaagaaac
     1861 aggagaaaaa tgatggattt acttttagca attgtattga ttgtgctagc ttttctagga
     1921 ggagctcttg gaggaatgta cttggttcgt aagcaaatcg aaaaagaatt tgctgacaac
     1981 ccacgtttga atgctgaagc agttcgtact cttttgagtg caaatggtca aaaaccaagc
     2041 gaagctaagg tacaacaagt ttaccaccaa atcatccgcc aacaaaaggc agcccttgct
     2101 aacaataaaa agaaaaaata aataaggaaa agtctgggat gaaagttcca gacttctcac
     2161 tatgttggtt atggtttagg gatgagactt tttccttgca ttctattgat atttgattat
     2221 gattaagaga gaggtagttg acattctatc aagctgtcgg tcagttcgtt cagtacaagg
     2281 aatcataatg aacgatcaat cagaaaaaag actagaaaga agactgtatg gataatcgac
     2341 caattggttt tttggattcg ggtgtcgggg gcttgaccgt tgtgcgcgag ctcatgcgcc
     2401 agcttcccca tgaagaaatc gtctatattg gagattcggc gcgggcgccc tatggccccc
     2461 gtccagctga gcaaattcgt gaatatactt ggcagctggt caactttctc ttgaccaagg
     2521 atgtcaaaat gattgtcatt gcttgtaaca ctgcgactgc ggtcgtctgg gaagaaatca
     2581 aggctcaact agatattcct gtcttgggtg taattttgcc aggagcttcg gcagccatca
     2641 agtccagtca aggtgggaaa atcggagtga ttggaacgcc catgacggta caatcagaca
     2701 tataccgtca gaaaatccat gatctggatc ccgacttaca ggtggagagc ttggcctgtc
     2761 ccaagtttgc tcccttggtt gagtcaggtg ccctgtcaac cagtgttacc aagaaggtgg
     2821 tctatgaaac cctgcgtccc ttggttggaa aggtggatag cctgattttg ggctgtactc
     2881 attatccact ccttcgccct attatccaaa atgtcatggg gccaaaggtt cagctcatcg
     2941 atagtggggc agagtgcgta cgggatatct cagtcttact caattatttt gaaatcaatc
     3001 gtggtcgcga tgctggacca ctccatcacc gtttttacac aacagccagt agccaaagtt
     3061 ttgcacaaat tggtgaagaa tggctggaaa aagagattta tgtggagcat gtagaattat
     3121 gacaaataaa atttatgaat ataaggatga ccaggattgg tatgttgggt cttatagtat
     3181 ttttggtggc gttaacagtt tgagcgacta taagacagat tttcctctgt ttgaattctc
     3241 caaaatattt ggagatgaag agtatggttt cccgctttca gttactgttt tacgctatgg
     3301 ttctacctac cgtttgttct cctttgtggt agacatgctt aatcaagaaa tgggacgaaa
     3361 cttggaagtt attcaacgtc atggggccct gctcttggtt gaaaatgggc aactcttgta
     3421 tgtagaattg cctaaagaag gggtcaatgt tcatgatttc tttgagacaa gcaaggtcag
     3481 agaaaccttg ttgattgcga ctcgtaacga aggcaaaacc aaggaattcc gagctatctt
     3541 tgataagtta ggctacgatg tggaaaatct taatgactac cctgacctgc ctgaagtagc
     3601 agaaacaggt atgacctttg aagaaaatgc ccgccttaag gcagaaacca tttctcaatt
     3661 aacgggcaag atggttttgg cagatgattc tggtctcaaa gtcgatgtcc ttggtggctt
     3721 accaggcgtc tggtcagctc gtttcgcagg tgtgggagca actgaccgtg aaaataatgc
     3781 caaactcttg cacgaattgg ccatggtctt tgaactcaag gaccgctcgg ctcagttcca
     3841 cacaacccta gtcgtagcca gcccaaataa ggaaagttta gttgttgaag cagactggtc
     3901 aggttatatt aactttgaac ctaagggtga aaatggcttt ggctatgatc ccctcttcct
     3961 tgtaggagag acaggtaagt catcagctga attaaccctg gaagaaaaaa atagtcaatc
     4021 tcaccgtgcc ttagccgtta agaaactttt ggaggtattt ccatcatggc aaagcaaacc
     4081 atcattgtaa tgagcgattc ccatggcgat agcttgattg tggaagaagt ccgtgatcgc
     4141 tatgtgggca aagtcgatgc cgtttttcat aacggcgatt ctgaactacg tccggattct
     4201 ccactttggg agggcatccg cgttgttaaa ggaaacatgg acttctacgc cggctaccca
     4261 gaacgtctgg tgactgagct tggttcgacc aagattatcc aaactcatgg tcacttgttt
     4321 gacatcaatt tcaactttca aaagttggac tactgggctc aggaggaaga ggccgctatc
     4381 tgcctctatg gtcacttgca tgtgccaagt gcttggttgg aaggcaagat cctctttcta
     4441 aatccaggtt ctatcagtca accacgaggt accatcagag aatgtctcta tgctcgtgtg
     4501 gagattgatg atagttactt caaagtggac tttttgacac gagatcacga ggtgtatcca
     4561 ggtttgtcca aggagtttag ccgatgattg ccaaggagtt tgagactttc ttgttggggc
     4621 aggaggaaac ttttttgacc cctgctaaaa atctagctgt gttgattgat acccacaatg
     4681 cggatcatgc gaccctcttg ctcagtcaga tgacctatac ccgtgttccc gttgtgacag
     4741 atgaaaaaca gtttgttggg acgattggac tcagagatat tatggcttat cagatggagc
     4801 atgacttgag ccaagaaatc atggcggata cggatatcgt tcatatgaca aaaacggacg
     4861 tagcggttgt ttcgcctgat ttcaccatta cggaggtctt gcacaagcta gtagatgagt
     4921 ccttcttacc ggttgtggat gcagagggta ttttccaagg gattattacg cgtaagtcca
     4981 tcctcaaggc cgttaatgcc ctcttgcatg actttagtaa ggaatatgag attcgatgcc
     5041 aatgagagac aggatttcag cctttttaga ggaaaagcag ggcttgtctg tcaattccaa
     5101 gcagtcctat aagtatgatt tggagcaatt tttagacatg gtaggtgagc ggatttctga
     5161 gaccagtctc aagatttacc aagcccagct agccaatcta aaaatcagcg cccagaagcg
     5221 aaagatttcg gcctgtaacc aatttctata ctttctctat caaaaaggag aggtggacag
     5281 cttttaccgc ttggaattag ccaaacaagc tgaaaagaag acggaaaagc cagagattct
     5341 atacctagac tctttttggc aggaaagcga ccatccagag ggccgcttgc tagcgctctt
     5401 aatcctagaa atggggctct tgcccagtga gattttagcc atcaaggttg cggacatcaa
     5461 tctggatttt caggtgttgc gaatcagcaa ggcttcccaa cagaggattg tcaccattcc
     5521 cacggccttg ctttcagaat tggaaccctt gatggggcag acctatcttt ttgaaagagg
     5581 agagaaaccc tattctcgtc agtgggcctt tcgtcagtta gaatcttttg tcaaggagaa
     5641 aggttttcca tccttatcag ctcaagtctt acgtgaacag tttattctaa gacaaataga
     5701 aaacaaggtc gatttgtacg aaattgcaaa aaaattagga ttaaaaacag tcctgacctt
     5761 agaaaaatat agataatgga tattaaatta aaagattttg aaggccccct ggacttgctc
     5821 ttgcatctgg tttctaagta ccagatggat atctacgatg tgcccattac ggaagtcatc
     5881 gaacagtatc tagcctatgt ttcaaccctg caggccatgc gtctggaagt gacgggtgag
     5941 tacatggtca tggctagtca gctcatgctg attaagagtc gtaaactcct tccgaaggta
     6001 gcagaagtga cagacttggg ggatgacctg gagcaggacc tcctctctca aatcgaagaa
     6061 tatcgcaagt tcaagctctt gggtgagcac ttggaagcca agcaccaaga acgggcccag
     6121 tattattcca aagcgccgac agagttgatt tacgaagatg cggagcttgt gcatgacaag
     6181 acgaccattg acctcttttt ggctttttca aatatcctag ccaagaaaaa agaggagttt
     6241 gcacaaaatc acacgactat cttgcgggat gagtataaga ttgaggacat gatgattatc
     6301 gtgaaagagt ccttgattgg acgagatcaa ttgcgcttgc aggatttgtt caaggaagcc
     6361 cagaatgtcc aagaggtcat caccctcttt ttggcaaccc tagagttaat caaaacccag
     6421 gagctgatcc tcgtgcaaga ggagagtttt ggagatatct atctcatgga aaagaaggaa
     6481 gaaagtcaag tgcctcaaag ctagacttga tagagaggaa agatgagtac tttagcaaaa
     6541 atagaagcgc tcttgtttgt agcgggtgaa gatgggattc gggtccgcca gttagctgaa
     6601 ctcctctctc tgccaccgac aggcatccag cagagtttag gaaaattagc ccagaagtat
     6661 gaaaaggacc cagattccag tttggctttg attgagacaa gtggtgctta tagattggtg
     6721 accaagtctc aatttgcaga gattttgaag gaatactcta aggcgcctat caaccagagc
     6781 ttgtctcggg ctgcccttga gaccttgtcc attattgcct acaaacagcc gattacgcgg
     6841 atagaaattg atgccatccg tggagttaac tcgagtggag ccttggcaaa gttgcaggct
     6901 tttgacctga taaaggaaga cgggaaaaag gaagtattgg ggcgccccaa cctctatgtg
     6961 actacggatt atttcctaga ttacatgggg ataaaccatt tagaagaatt accagtgatt
     7021 gatgagcttg agattcaagc ccaagaaagc caattatttg gtgaaaggat agaagaagat
     7081 gagaatcaat aagtatattg cccacgcagg tgtggccagt aggagaaaag cagaagagct
     7141 gattaagcaa ggcttggtga cggttaacgg ccaagtggtg cgtgaactag caaccactat
     7201 caagtcaggc gacaaggtcg aagttgaagg tcaacctatc tacaacgaag aaaaggtcta
     7261 ctatctgctt aacaaaccac gcggtgtgat ttccagtgtg acagatgata agggtcgcaa
     7321 gacggttgtc gacctcttgc ccaatgtcaa agagcgtatt taccctgtgg gtcgtttgga
     7381 ctgggataca tcaggtgtct tgattttgac caatgatggg gactttacag acgagatgat
     7441 tcaccctcgt aatgagattg acaaggttta tgtcgcgcgt gttaagggtg tggccaataa
     7501 ggacaatctc cgccccttga cccgtggtct tgagattgat ggtaagaaaa ccaagccagc
     7561 tgtttatgaa attctcaaag tggacccagt caaaaatcgc tctgtggtgc agttgaccat
     7621 ccatgaaggg cgtaaccatc aggttaaaaa gatgtttgaa gctgttggtc tccaagtaga
     7681 taagttgtct cggactcgtt tcggacacct agacttgaca ggactccgtc caggagaatc
     7741 ccgtcgtctt aataaaaaag aaatcagcca actacacacc atggctgtaa ctaagaaata
     7801 atgaaacgaa ttttaatagc gcctgtgcgc ttttaccaac gttttatctc accagtcttt
     7861 ccaccctctt gtcgctttga gctgacttgt tccaactaca tgattcaggc tattgaaaaa
     7921 catggtttta agggggtatt gatgggcttg gctcggattt tacgttgtca tccctggtcg
     7981 aaaacaggta aggaccccgt tccagaccac ttttccctta aacgaaatca agaaggggaa
     8041 tgaggtgggg taaatagatt tcaaaatgat aaaaacgcat cctatcaggt ttgagtgaac
     8101 ttgataggat gcgttttaga atgtcaaaat tttatactct tcgaaaatcc cttcaaaccg
     8161 cgtcagcttt catct
//
LOCUS       DAVBHS010000094         8108 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVBHS010000094 DAVBHS010000000
VERSION     DAVBHS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8108
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.094"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..1009
                     /locus_tag="VQ651_001488"
     CDS             <1..1009
                     /locus_tag="VQ651_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HET2754465.1"
                     /translation="LGGLCVIGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDL
                     MKRFGSERLKGIFERLNMSEEAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMR
                     EQREIIYAQRYDVITADRDLAPEIQAMIKRTIERVVDGHARAKQDEKLEAILNFAKYN
                     LLPEDSITMEDLSGLSDKAIKEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVD
                     NKWTDHIDALDQLRNAVGLRGYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQ
                     IHEQERPQAERHISTTATRNIAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKR
                     Q"
     gene            1090..2121
                     /locus_tag="VQ651_001489"
     CDS             1090..2121
                     /locus_tag="VQ651_001489"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2754466.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            2123..3154
                     /locus_tag="VQ651_001490"
     CDS             2123..3154
                     /locus_tag="VQ651_001490"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2754467.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            3193..3561
                     /locus_tag="VQ651_001491"
     CDS             3193..3561
                     /locus_tag="VQ651_001491"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET2754468.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            3551..4654
                     /locus_tag="VQ651_001492"
     CDS             3551..4654
                     /locus_tag="VQ651_001492"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET2754469.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLALPYDLIPALTLESALVHIKTVPAGACMGYGATYQTDSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            4673..6688
                     /gene="recG"
                     /locus_tag="VQ651_001493"
     CDS             4673..6688
                     /gene="recG"
                     /locus_tag="VQ651_001493"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET2754470.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     IKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            7004..7984
                     /locus_tag="VQ651_001494"
     CDS             7004..7984
                     /locus_tag="VQ651_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HET2754471.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
BASE COUNT         2340 a         1602 c         1969 g         2197 t
ORIGIN      
        1 acttggagga ctttgtgtta ttggtacaga acgtcatgaa agtcgtcgta tcgataacca
       61 gcttcgtgga cgttcaggtc gtcaaggaga tccaggtgag tcacaattct acctatctct
      121 tgaagatgat ttgatgaaac gttttggttc tgaacgcttg aagggaatct ttgaacgttt
      181 gaacatgtct gaagaggcga ttgagtctcg catgttgacg cgtcaggttg aagcagctca
      241 gaaacgtgtc gaaggaaata actacgatac ccgtaaacaa gtccttcaat acgatgatgt
      301 catgcgtgaa caacgtgaga ttatctatgc tcaacgttac gatgtcatca ctgcagatcg
      361 tgacttggca cctgaaattc aggctatgat caaacgcacg attgaacgtg tcgttgatgg
      421 tcatgcgcgt gccaaacaag atgaaaaact agaggcaatt ttgaactttg ctaagtacaa
      481 cttgcttcct gaagattcta ttacgatgga agacttgtca ggcttgtctg ataaggccat
      541 caaggaagag cttttccaac gtgccttgaa ggtttacgat agtcaggttt caaaactacg
      601 cgatgaagaa gcagttaaag aattccaaaa agttttgatt ctacgagtgg tggataacaa
      661 gtggacagat catatcgatg cccttgatca attgcgtaac gcggttggac ttcgtggcta
      721 tgctcagaac aaccctgttg ttgagtatca ggcagaaggt ttccgtatgt ttaatgatat
      781 gattggttcg attgagtttg atgtgacacg cttgatgatg aaagcacaaa ttcatgaaca
      841 agaaagacca caggcagaac gtcatatcag tacaacagcg actcgcaata tcgctgctca
      901 ccaagcaagt atgccagaag atttggattt gagccagatt ggacgcaatg aactttgccc
      961 atgtggttct ggtaagaaat ttaaaaactg tcacggtaaa agacaataaa atgagatagt
     1021 ttagaggcgg atatcttgtg aaaagtaaat ttttactggg tatccgtttg ctttataagg
     1081 agatgagtta tggtatttac agcaaaaagc tctaaaataa atatagaaga agttcgtgcc
     1141 ttgtcaaaat tagaaggtca ggctttggag aggaaatcac agcgcgatca agagctagaa
     1201 gccattatac gtggagaaga tcagcgaatt ctcttggtaa tcgggccatg ctcatctgac
     1261 aacgaagaag ctgtccttga atacgctaag cgtttggcag tcctacaaga agaagtggca
     1321 gatcgtatct ttatggttat gcgtgtttat actgccaaac cccgtaccaa cggagatggc
     1381 tataagggct tgattcacca gcctaacgcg acagaagcgc ctagtcttat caatggaatc
     1441 aaagccgttc gccatcttca ctatcgtgtc atcacagaaa cagggatgac aactgctgat
     1501 gaaatgcttt atcctgaaaa ccttccgctt gtagatgatt tgatttctta catggcagtt
     1561 ggtgcccgtt cagttgaaga ccagcaacac cgctttgtgg caagtggggc aggattttct
     1621 actggtttta aaaatccaac ctctggaaat ctcaatgtca tgtttaatgg gatttatgct
     1681 gctcaaaaca aacaaagttt ccttttctta ggaaaagaag tagaaacaac tgggaacccg
     1741 ctttcacacg ctattcttcg tggtgctctt aatgagtatg gaaaaaatat tcccaactac
     1801 tattatgaca atttaattga taccattgcc caatatgaga aaatgggctt ggaaaatcct
     1861 tttatcatca ttgataccaa tcatgacaat tctggtaagc agtatattga acagatccga
     1921 attgtccgcc agaccttgat taaccgtgct tggaatgaaa aaattaagca gttcgttcgt
     1981 ggttttatga ttgagtctta tctggaagat ggtcgacaaa atgagccaga agtatttggc
     2041 aagtctatca cagacccttg cctgggttgg gataacacag aagctcttgt cagagaaatt
     2101 tacaaaacgt taggagaata agatggcatt tattgaaaaa ggtcaagaaa tcgatatgga
     2161 agtcatcaag gctgaaaccc aattgtctgc ggaagccttg agactcaagg aaagccgtga
     2221 cagggaattg gcagatatta tttcagggga agatgaccgt attctcttgg tgattggtcc
     2281 ttgctcttct gataatgaag aggcggtctt ggaatatgct cgccgtttat ccgccttgca
     2341 aaagaaggta gcggacaaga ttttcatggt catgcgcgtg tatactgcta agcctcgtac
     2401 caatggagac ggctataaag gattggttca ccagccagat acttctaagg ctccaagcct
     2461 gattaacggt ttgcaggctg tgcgccagtt gcattaccgc gtgattacag agactggttt
     2521 gacaacggca gatgagatgc tttatccgtc aaatctgatc ttggtggatg acttggtcag
     2581 ctaccatgcc gttggagctc gttctgtgga agaccaagag caccgctttg tggcttctgg
     2641 gattgatgca ccagtaggga tgaaaaatcc aacctcagga aatttgggtg ttatgtttaa
     2701 cgccatctat gctgctcaaa acaagcaaac cttcctttat catgggcagg aagttgagac
     2761 atcaggtaat cctttggccc atgttatcct ccgtggagca gtcaacgagt atggcaatta
     2821 tatgccgaat tactactatg aaaacctact ccaagccatt gaacgctatg aaaccatggg
     2881 acttgaaaat ccttttatcc tcattgacac caaccatgat aactcaggca agcaatatat
     2941 ggagcagatt cgaattgttc gccagacctt gcagaatcgt gattggaatg agaaaattaa
     3001 aaagacggtt cgaggattta tgattgaatc ttacctagca gatggtcgtc aaaaccaacc
     3061 agagatcttt ggttgctcta ttactgaccc ttgcctaggt tgggaaaata cagaggcctt
     3121 ggtagaagag atttatgtta ccttgacaaa ataagtgaaa aggatggagt tggggaatct
     3181 caactccttt tgatgagaat gatagttgga cacggaattg acatcgaaga attggcttcg
     3241 atagaaagcg cagttacacg acatgaagga tttgctaagc gtgtactgac cgctcaggaa
     3301 atggagcgct tcaccagtct caaaggacgc aggcaaatag aatatttagc tggtcgctgg
     3361 tcggctaagg aggccttttc caaggctatg ggaacgggca ttagcaagct cggttttcag
     3421 gatttggaag tattgaacaa tgaacgtggg gcgccttatt ttagtcaggc accattttca
     3481 ggaaagattt ggctgtctat cagccacacc gatcagtttg tgacagccag tgtcattttg
     3541 gaggaaaatc atgaaagcta gtccacatag accaaccaag gctctgattc atctgggagc
     3601 tattcgacaa aatattcagc aaatgggggc tcatatccct caaggaacgc tcaagttggc
     3661 tgtggttaag gccaatgctt atggtcatgg agctgttgcc gttgccaagg caattcaaga
     3721 tgatgttgat ggcttttgcg tttccaatat cgatgaagcc attgaactca gacaagctgg
     3781 actcagcaag ccaatcctca ttttaggagt ttctgaaatc gaagctgttg ctctagctaa
     3841 agaatatgac ttcaccttga cagtggctgg actggagtgg attcaagcac tcttagataa
     3901 ggaagtggac ctaactggat tgacagtcca cctcaagatt gattcaggga tgggacggat
     3961 tggttttaga gaggcaagtg aggttgagca ggctcaagat ttgctccaac aacacggtgt
     4021 tcgtgttgaa ggaatcttta cccactttgc tactgctgat gaggaatcag atgactattt
     4081 taatgcccag ttagaacggt ttaaaactat tttagctagt atgaaggaag ttccagagct
     4141 ggttcatgct agcaattctg caacgactct ttggcatgta gagactattt tcaatgcggt
     4201 tcgtatggga gatgccatgt atggtctcaa tccaagtgga gcggtcttgg ctttgcctta
     4261 tgatttgata ccggccttga ccttggagtc tgctctggtt catatcaaga cagttccagc
     4321 tggtgcttgc atgggctatg gagcaactta tcaaacggat agcgagcaag tcatcgcgac
     4381 cgtgccaatc gggtatgcag atggatggac aagagacatg caaaatttct ctgtcttggt
     4441 agatggccaa gcttgcccaa ttgtcggcag ggtttcgatg gaccaaatca ctattcgatt
     4501 gcctaagcct tacccactcg gaaccaaggt aaccttgatt ggctccaatg gggataagga
     4561 aatcactgca actcaggtag cgacctaccg cgtaaccatt aactatgagg tggtttgcct
     4621 cctcagcgac cgtattccga gagaatatta ctagaaaaga aaggagtgga gcatgaatct
     4681 acatcaaccc ttgcatgtct tgcctggtgt gggaccaaag tcagcagaaa aatacgccaa
     4741 actaggaatt gaaaacttgc aagatctctt gctctacttt cctttccgtt atgaagactt
     4801 caaaatcaag caggtgctgg agctggaaga cggtgagaag gcagttcttt ctggtcaggt
     4861 agtgactcct gctagtgtcc agtattatgg tttcaagcgc aatcgcctgc gttttagtct
     4921 caagcaggga gaggtcgttt ttgcggtgaa tttctttaac cagccctatc tggctgataa
     4981 aatagagttg ggagcaaccc ttgctgtctt tggaaaatgg gaccgcgcta aggctagtct
     5041 gactgggatg aaggttctgg ctcaggtaga agatgacctc cagcctgtct atcgtctggc
     5101 tcagggaatc agtcaggcca gtctggttaa ggtcatcaag acggcttttg atcagggact
     5161 ggacctcttg atagaagaaa atctgcccca gtctttacta gacaaataca aactcatgtc
     5221 ccgttgtcag gcagtccgag ctatgcattt tccaaaggat ttggcagaat acaagcaggc
     5281 tcttcgccgt atcaagtttg aggaactctt ttatttccaa atgcagctgc agatgctcaa
     5341 gtctgaaaat agagttcagg gaagtggtct ggttctgaat tggtctcagg aaaaagtgac
     5401 agcagttaaa gtaagtcttc cttttgccct gacccaagct caggaaaaga gtttgcagga
     5461 aattttaact gatatgaagt ccgaccacca catgaatcgt ctcctacaag gggatgtggg
     5521 gagtggaaaa acggtagtcg ctggcttggc catgtttgcg gcagtgacag caggttatca
     5581 ggctgcccta atggtaccaa cagaaatcct cgcagagcaa cactttgaga gtttacagaa
     5641 cctttttccc aatttgaaac tggctctctt gacaggttcc ttgaaagctg cagaaaagag
     5701 agaggtcttg gagaccattg ccaagggtga ggctgatttg attatcggaa ctcacgctct
     5761 gatacaagat ggggtggagt atgctcgtct tggtttgatt attatcgatg agcagcaccg
     5821 ttttggtgta gggcaaaggc gtattttacg ggaaaaaggt gacaatccag atgtcctcat
     5881 gatgacggcg actcccattc cacggacgct tgccatcaca gcctttggag atatggatgt
     5941 ttccattatc gaccagatgc cagcaggtcg gaagcctatt gtgacgcgct ggatcaaaca
     6001 tgagcaacta cctcaggtct tgacttggtt agagggggaa attcaaaaag gttcccaagc
     6061 ctatgtcatc tctcctttga ttgaagaatc agaagctcta gatttgaaaa atgccattgc
     6121 cttatcagag gagttgacga ctcattttgc aggcaaggca gaggtggctc ttctacatgg
     6181 taggatgaag agtgacgaaa aagaccagat catgcaggat ttcaaggaga gaaagacgga
     6241 tattctggtt tcgacgacgg ttattgaggt tggggtcaac gttcccaatg cgactgtcat
     6301 gattatcatg gatgccgatc gcttcggtct cagtcaactt caccagctta gaggtcgtgt
     6361 cggtcggggg gacaagcagt cctacgctgt tctcgttgcc aatcccaaga cggattctgg
     6421 gaaagaccgc atgcgtatca tgacagaaac gaccaatgga tttgtccttg cggaggaaga
     6481 tttgaaaatg cgtggttctg gtgagatttt tggaaccaga cagtcaggac ttccagagtt
     6541 ccaagtggct gatattatcg aagattttcc gattttagaa gaagcaagaa aggttgctag
     6601 ctacattagt tctatagaag cttggcaaga agatccagag tggcgcatga ttgcccttca
     6661 tctggaaaag aaagaacatc tggattaagc tttctctaag gaaaacttaa gctagttttt
     6721 aggtttggct cttatactaa agtcatcaaa aagaaacgag gactctcata tgacagtaac
     6781 gattaaagta aattaccaaa ccactttcca aaagaaggaa gcaaaaaact agtataaaca
     6841 gaagagagag cgaaatgctc tttttcgttt ctaaaactac tttcagccca tcatcctaaa
     6901 agtaaagaat ctaaattcac tttctattta tccttctttc ttgcattgat tacatagata
     6961 tgctacagtt gtggtaacga ttacaaaata aaaggagcat gctatgaaaa atccagcttt
     7021 gctagaagaa attaagacct atagaggaag ggatgaggtt ccggaagact ttgatgcttt
     7081 ctgggatggg gaagtgaaaa atgtttccac gcttccatcc taccacttgg aggaaagaga
     7141 tttccacatt cctcaagtca agtgctatga gttaacattt gaaggaagca aggaaggaaa
     7201 ggtctatgca cgcattgttc ttccaaagag tgaggagaag gttccactaa tcttccattt
     7261 tcatggttat atgggacgtg gctgggactg ggccgacatg ctgggcttca ccgtagctgg
     7321 ttacggtgtt gtttccatgg atgtgcgggg ccagtcaggt tactcacaag acggcttgcg
     7381 ttctccttta ggaaataccg tgaaggggca tattatccgt ggtgctatgg aaggtcggga
     7441 ccacctcttt tataaggatg tttatctgga tatttaccag ttggtcgaaa ttgttgctag
     7501 tctgtctcag gttgatgaga agcgtctttc tagctatggt gcctcacaag gaggggctct
     7561 agctctagtt gcagcagcgc tcaatcctcg aattcagaaa acagttgcca tttatccctt
     7621 cttgtcagac ttcagacggg tgattgagat tggtaatact agcgaggctt acgacgaact
     7681 tttccgttat ttcaagtttc acgacccctt ccatgaaaca gaggaggaaa tcatggcgac
     7741 ccttgcctat atcgatgtca aaaatcttgc ccatcgtatc caaggtgagg ttaagatgat
     7801 tacgggcttg gacgacgatg tttgctatcc cattacccag tttgcgattt ataatcgtct
     7861 gacctgcgat aaaacctatc gcatcatgcc tgagtatgct cacgaagcca tgaatgtatt
     7921 tgtcaatgac caagtctaca actggctctg tggaagtgag attcctttta aatatctaaa
     7981 ataaggagtc gactctaagc acaaaatctt aaaaattaca aacacgcata gtatcagggg
     8041 attaaaaaaa cttgatacta tgcgttttat catggaaata tagtaaaatg aaacaagaat
     8101 aggacaaa
//
LOCUS       DAVBHS010000095         8073 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVBHS010000095 DAVBHS010000000
VERSION     DAVBHS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.095"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(135..1103)
                     /locus_tag="VQ651_001495"
     CDS             complement(135..1103)
                     /locus_tag="VQ651_001495"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET2754472.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1248..2063)
                     /locus_tag="VQ651_001496"
     CDS             complement(1248..2063)
                     /locus_tag="VQ651_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET2754473.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFTNTYIHDLLGVLFPD
                     SPFLNAFESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIG
                     YIRTDLPEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAHKEQKVGKKQGLI
                     FLGLALRTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(2088..2585)
                     /locus_tag="VQ651_001497"
     CDS             complement(2088..2585)
                     /locus_tag="VQ651_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET2754474.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2658..4019)
                     /gene="radA"
                     /locus_tag="VQ651_001498"
     CDS             complement(2658..4019)
                     /gene="radA"
                     /locus_tag="VQ651_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET2754475.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MSVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4033..4548)
                     /locus_tag="VQ651_001499"
     CDS             complement(4033..4548)
                     /locus_tag="VQ651_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2754476.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGFPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4550..4993)
                     /locus_tag="VQ651_001500"
     CDS             complement(4550..4993)
                     /locus_tag="VQ651_001500"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET2754477.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(5050..5147)
                     /gene="ffs"
                     /locus_tag="VQ651_001501"
     ncRNA           complement(5050..5147)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VQ651_001501"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5180..5647)
                     /locus_tag="VQ651_001502"
     CDS             complement(5180..5647)
                     /locus_tag="VQ651_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET2754478.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(5848..7134)
                     /locus_tag="VQ651_001503"
     CDS             complement(5848..7134)
                     /locus_tag="VQ651_001503"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET2754479.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGIGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGARNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(7365..7601)
                     /locus_tag="VQ651_001504"
     CDS             complement(7365..7601)
                     /locus_tag="VQ651_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HET2754480.1"
                     /translation="MLQKIYEQMTNFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            7867..8073
                     /locus_tag="VQ651_001505"
                     /pseudo
     CDS             7867..8073
                     /locus_tag="VQ651_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2585 a         1946 c         1290 g         2252 t
ORIGIN      
        1 tgatgacgta cagtcatatt tcttctcttt ttattatatc acagttttaa atctagcttt
       61 actagattca ccgctactat ctatttattc ggaaaaaaga cgaaaaaacc tgagaatcat
      121 ctcaggcttg gtcattaaat ttttttctca atatcgaaaa gtggagaaag tggtcgtttt
      181 tcatgaatac gtacgatagc atcccctagg agatgagcga ttgaaatctg ctcaatctta
      241 tcaatcaaac gctcttctgg cagatagatg gtatccaaaa caaccaattt cttaatagct
      301 gatttttgga tattgtccgt agcaggacca gaaagaactg ggtgcgtaca gcttgcatag
      361 acttcaacag caccagcttc cgcaagagca tctgccgcat gacaaatcgt tccagcggta
      421 tcaatcatat catcaatcaa gatacaagtc ttgccttcaa ccttaccgat gatgttcata
      481 acttcactag tattcatctt atcaacgcta cgacgtttat caataatagc gatagatgtt
      541 ttcaaaaatt ctgccaactt acgagcacga gtcacccctc catggtccgg gctgacaacc
      601 acatagtcag aaccaaccat accacgacgc tcaaaataat ctgcaatcag aggagcaccc
      661 atcaaatgat ccacaggaat atcaaagaat ccttgaattt gcgcagcatg caagtcgatg
      721 gtcaataaac gatccactcc agctacttca agcatatttg cgacaagttt tgaagtgatt
      781 ggctcacgcg ctctcgcctt tctatcctga cgtgcatacc catagtaagg catgacaaca
      841 ttgacagatt ctgcactcgc acgcttcaaa gcatctacca taatcaaaat ttcaagcaga
      901 ttgtcattta caggcgaact agttgattgt aggataaaga cgtgtttccc acggattgat
      961 tcttcaatgt tgacctgaat ctctccatct gaaaattggc gaacacttga tttccccaac
     1021 tctatcccaa tctcctgcgc cacacgttct gccaattctt tattagaaga aagggcaaac
     1081 agctttaaat cagaaaaaga catgatttcc tccggtatat atgtatcgct tgtgcttttc
     1141 acaagatttt ccatctacca ttgtagcgct ttttgcacta tttttcaatc aaaaataaaa
     1201 gaagggcacc atatttgtac ccttgcatca ttcttttgaa aaatattcta ggtcatcaac
     1261 tcattgtgtt tctcaacaaa gcaataagca tgataaaaac catagagagc aatagccgta
     1321 accactggaa tcgctaaagg caactctgtt tccaactcca caaaaggaga gttaaacaag
     1381 aagtgagttc tcaaggctaa acctagaaaa ataaggccct gtttcttgcc aaccttctgt
     1441 tctttatggg ctctgtaaag caagtaaaca cctactacag ctagacctga aaaagtccag
     1501 tgagaggcaa ttcctgagat gatacgctct aaaattcgcg aaatagtaaa gtcaaagccc
     1561 tctggcaaat ccgtacgaat ataaccaata tcctcaatca tttggaatcc caaaccggaa
     1621 gcaattccaa gtaaaaacaa agattttaat tttcgcacag gaatcaaagc caaaacaaaa
     1681 acaagtggca ataatttcaa gggttcttct accaaaggag ccacaatagc actttcaaag
     1741 gcatttaaaa atggactatc tgggaaaaga acccccagta aatcatggat ataagtatta
     1801 gtaaaactag acaaccagcc tgaaaggaac atccctccca ataaagacag aatcaaaacc
     1861 ttctttggca attcccattt tttcccaata cggaagagaa aataaagagc cggaatcatg
     1921 taaaagagag ctagaaagat agaaactccc attagtccat attccgcacc tgacctcgaa
     1981 ccgtccgtat agtagatggt ttcatactgt aaaccaatac atagcaataa aataaaaata
     2041 aataaaatac tgcttttctt catacacttt ctttctaaat gaagtattta taattctacg
     2101 actgtcatac ttcctgtatc aacattgtaa atggcaccag agataatgac atcgtctggt
     2161 attaggggag actcgataag cagttgcata tcctcgcgta cactctcttc tatatcttgg
     2221 aagggcaaga agtcctggtc tgacacatct acacctaatt cctcttttaa atactcctga
     2281 aaaggttcat tttcaaaggt ctgagcacca cagtctgtat ggtgcaatac cacaatctct
     2341 cttgtcccca tttgttgctg ggaaataact agcgaacgaa tcatgtcttc agtcactcga
     2401 ccacctgcat tccgcaagat atgagcatcc ccaagtgcca agcccagagc ttgcgcaacg
     2461 tgcagacgag agtccataca ggtcacaata gctactcttg ttttgggttt aagtggcaga
     2521 tttaactgcc catgtagggc aacataagcc tgattagctt gcataaactg ttcaaaatac
     2581 gacacgattc cctccttgaa aatttgatag tcaaatattt ctcctatctt atcattttta
     2641 agagaatttg tcacggatta tgcaaagacc tttttcaaaa cttcctgaat cgttgtcacg
     2701 ccaatgacct gaatttcctt aggcagagtg attcctgtca aggaattctt aggtacataa
     2761 atcttagtaa agcccagttt agcagcttcg ttgatgcgtt gctcaatacg attcacgcgc
     2821 cgaatctctc ctgtcaagcc cagttctccg acaaaacatt cctgaggatt agttggcttg
     2881 tctttgtagc tcgaagcaat agcaactgca acagccaagt caatggcagg ttcatccaat
     2941 ttaacaccac cagcagattt gagataggca tcctgatttt gcaagagaag ccctgcccgt
     3001 ttttccaaaa cagacataat caagctagca cggttaaaat caagtcctgt cgtagtacgc
     3061 ttggcatttc caaacatggt cggtgttacc aaagcctgaa cctccgccaa aatcggacgc
     3121 gtcccttcca tggttacaac gatggaggaa ccagtcgccc catccaaacg ctcttctagg
     3181 aaaacttgac tcggattgag tacctcaacc aagccgcccg actgcatctc aaaaatccca
     3241 atctcattag tggaaccaaa acgatttttg accgctctca aaatacgaaa ggtgtggtga
     3301 cgctcccctt caaagtaaag caccgtatcc accatatgct ccaacatacg aggcccagcc
     3361 aaggttcctt ctttggtcac atgacctacg ataaagatgg caatgttatt ggtcttggcc
     3421 aactgcatga gttcagcggt cacttcacgc acctgagaaa cagacccctg cacccctgaa
     3481 atctcaggag acatgatggt ctggatggaa tcaataatga gaaagtctgg ctggatacgc
     3541 tccacttctg cacgaacact ctgcatattg gtctctgcat agagataaaa ctcactatca
     3601 atatcaccta agcgctctgc acgtagttta atctgctggg cagactcctc cccactgaca
     3661 tagagaactg tccccacttg ggacaactgg gttgagactt gtaggagaag agttgatttc
     3721 ccaatcccag gatccccacc gatgaggacg agacttcctg gtaccactcc gcctccaagc
     3781 acacggttga attcctccat ctccgtcttg gttcgattga cattgatgga agtcacctca
     3841 gctagtttca tgggcttggt tttctcacct gtcaaggaca cacgcgcatt cttgacctcg
     3901 gcaacctcaa cctcttccac aaaagaagac caagacccac agttggggca acgtcccaga
     3961 tatttagggg aattataccc acaattttga catacaaatg tcgctttttt ctttgcgatg
     4021 acaaacctct ttctatatct ctaactcaca ctcaatcact tggcaaaaat caatcttctc
     4081 atttggcaca aactggcgca tgagcattcg atgagcaaca actaccacag tctgatgttc
     4141 tcgatactta gacatacatt ctagaaaccg agacttcatt tccgtagctg tctcatattg
     4201 aataggacta ttaggaagca actccccctt gttttctaaa aacagtcttc tagctgtttc
     4261 aaagttttct attcctgttt tatagacctg ccattcatgt aataaaggct ctactcttaa
     4321 aggaaaaccc gtagcacata ccacatacga agccgtttct aaagctcttg tgactgcaga
     4381 agatacgatt atttcagctg acgagagtaa aggatttttg ctcaatttct ggacttgctg
     4441 ccgtcccatc tcagacaagg gtgccaaatc tatcccaaat cctatataag aacgctcctc
     4501 taactcacgg taatctggct ccccatgacg tacaaagata atcttcattc tagtgccctg
     4561 tcgatccaaa tccaccagtt cgaacgccat cagccgcatc tccatctgca attaagaaag
     4621 tagcaaaaac agcctggaca atacgctccc caacttcaag aacaacctct tggtctgtga
     4681 tattcttcat ctgcgcaaaa atatgccctt catttccagg atttccataa taatccccat
     4741 caatgactcc aactgagtta attaaaacca agcccttctt acgaggattt gaagaacgat
     4801 catagaggta gagaacctca gtcggctgca tataagcctt aacccctgtc ggaaccaaga
     4861 caatctctcc tggcgcaaca actgtacgca cagcaacctt taagtcgtaa ccagccgcat
     4921 gcgctgtctc acgcttgggc aataaatttt catctgtaaa actcgaaacc aattcaaaac
     4981 cacgaatttt cataattttc tcttttctat tatcatttat tctagattat tctatcttat
     5041 ttattcggaa aaagcacgaa aaaaagagca cacaattcaa atcgcttagg gctgctggat
     5101 tcctcccctg acccgcttca cgcagaactg ttgctccact atttattata tcacattcct
     5161 attcatttta aaagcaaaat tattttttcc gtctatttct aaaaaaatcc tgcatgatag
     5221 ctgcgcattc atcttccaaa attcctattt caacctccac acgatgatta agacgctcat
     5281 ctgtcaagat atcgtacaaa cttccagcag cgccaaattt ctggttttta gccccataga
     5341 ccacatttgg aatgcgggca agcccaatcg ccccactaca catgacacaa ggttcaatgg
     5401 tcacaaaaag tgtgcaatcc agcaagcgcc agctctcctc actcaagttc gcatcctcta
     5461 tagccataat ttccgcatgc ataaccgctc gctgtaattc ctcacgcgca ttatgcccac
     5521 gaccaatgat ttccccatct ttgacaatca cacaaccaat tggaatttca tcgtgttcaa
     5581 gagcaatctc agcctctctc aaagcctccc tcataaagac ttctttttct tcaagcgtat
     5641 aatacatctc ttttctcttt tcctacttat cgattttatt attatatcat gaatcccaac
     5701 acaaaaaaag ccaccgaatg cggtgactct atagggagat tattatgaaa aagaaaagtt
     5761 taggatattt gttacaacaa gttaggaggt cttcttgtaa ctgtctatag tatacccgac
     5821 ctatcttaaa caaatcttaa aaatctctta ggaccaaaca ctttctaaaa tatttgtttg
     5881 ttcacgacca ggacctactg agaaagtaga aatacgaacg ccaaccaatt cactcacacg
     5941 acgaacatag ttacgcgcat tctcaggaag atcttccaaa ttgcgagctc cggtaatatc
     6001 ttctgaccaa cctggcaact cttcatagat aggcttgcaa cgtttcaatt gctcaagact
     6061 agctggatag tagtcaatac gttgaccgtc aagatcatag gccacacaaa ttttcacagt
     6121 atccaagccg ctcaaaacat caatagagtt caaagaaagg ttagtaatac cagaaacacg
     6181 acggctatga cgcatcacaa ctgagtcaaa ccaacctaca cgacgtggac gaccagttgt
     6241 tgtaccatat tcatgaccca cttcacggat acgttctccc acttcatcaa acaactcagt
     6301 tgggaaagga ccatctccta cacgactcgt ataagcttta catacaccta caaccttgtc
     6361 aatcttgctt ggaccgatac cagaaccaat tgtcacacca ccagctacag ggtttgatga
     6421 cgtaacaaat ggataagtac cttgatcgat atctagcata acaccttgtg caccttcaaa
     6481 aagcacacgt ttgccattat caagcgcatc attcaagata acagatgtat ctatcacgta
     6541 tttcttgatt tgttgaccat attcgtaata ttcttcaaaa atatcatcga aaacaatcgc
     6601 tttactgtca tacaattttt caaaaagacg attcttttca gcaaggttac gttctaaacg
     6661 ctcacggaaa atatctttat ctaaaagatc tgcaatacga attccaacac gagcagcctt
     6721 gtccatataa gctggaccaa ttcccttaat tgtcgtacca atcttattgt cgcccttagc
     6781 ttcttcttgc aagcgatcca actcgatatg ataaggcaaa ataacatgcg cacgatcaga
     6841 aatacgcaag ttatcagttg ttacaccttc ctcatgaaga tagctcaact cttttacaag
     6901 agatttagga tttacaacca taccattccc aatgacagat attttttcag ggaagaaaat
     6961 cccagatgga atcaagtgca acttaaattt cttaccgtca atcacaatcg tgtgaccagc
     7021 attatcacca ccttggtaac gtgcaattac ttctgcattc gctgaaagga agtctgtaat
     7081 cttcccttta ccttcatcac cccattgggt acctacaaca acaactgaag tcataatttt
     7141 gtctgagccc tcaggctctt cctttctcac atacatggca ggactctcac ctgcaattat
     7201 atcttacaat ttattataat aaaaaatcgc ctttttatca agaagaaaca atagaaagat
     7261 ttgctatttc caactattaa aaaatgattt agaaaaatta ctagctattt actattatct
     7321 ttccataaaa gagtaaatta gttcggaaat ttactaaaat tacctcaaca agaaataaaa
     7381 ccccgattca ttaccaattt ttcaagatac aaacgataag caacacgata atggtaaacg
     7441 ataaaatccc tacgacaacc aatgccatat ctcactaaat aataaattaa aaatttaaaa
     7501 tgaacatgtt cccagtcaaa attatcacca aatgtaggac catactcttc ttcaatacta
     7561 tcatagaaat tagtcatctg ctcataaatt ttttgtaaca taatcaaata ctccttttct
     7621 tttttataaa cttattctaa caaaaaattt tacacattca ctatcaattc ctgaattgtt
     7681 aaaataccag cctcaacaag ataaaaatag gaaaagttga caaaatagaa acaaatttgc
     7741 tacctaaatt tcaaacgatt aaagttctaa aaagaccgcc ttaaaacttt tgggtaaatc
     7801 ctattagaac tataatattt atatatatgt attatggttc tgattttcta gtataaaaaa
     7861 attcaaacta gaattcttct tagaaaaggc cgttttaagt gctatcattg ctcaaaaatg
     7921 gaggtcgctg agacttctat cgtcaagaag aatcatcaaa ttccttgtat catcaaccaa
     7981 aagattgctc agaagttaat tgaaaagact tctatgaccg acattgatca tcagttgtct
     8041 atttcaactt caactgtcat tcgcaagatc aat
//
LOCUS       DAVBHS010000096         7566 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVBHS010000096 DAVBHS010000000
VERSION     DAVBHS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7566
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.096"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            119..778
                     /locus_tag="VQ651_001506"
     CDS             119..778
                     /locus_tag="VQ651_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HET2754481.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            803..1870
                     /locus_tag="VQ651_001507"
     CDS             803..1870
                     /locus_tag="VQ651_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2754482.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            1973..2983
                     /locus_tag="VQ651_001508"
     CDS             1973..2983
                     /locus_tag="VQ651_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754483.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            2995..4686
                     /locus_tag="VQ651_001509"
     CDS             2995..4686
                     /locus_tag="VQ651_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2754484.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDPNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            4689..5399
                     /locus_tag="VQ651_001510"
     CDS             4689..5399
                     /locus_tag="VQ651_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET2754485.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            5495..5863
                     /locus_tag="VQ651_001511"
     CDS             5495..5863
                     /locus_tag="VQ651_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754486.1"
                     /translation="MKDYKINFDLGKIEYFDNNCLIQVYKFISFYDICEMVFAFHLPP
                     DELITNVIFKEKINSMLKCYIDRLLYVFINPTHFTEKVNLQFYGSFFSYEFICREVGN
                     ILKNKGVKCNLNFFEGEEYL"
     gene            5971..6972
                     /locus_tag="VQ651_001512"
     CDS             5971..6972
                     /locus_tag="VQ651_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2754487.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
BASE COUNT         2512 a         1023 c         1441 g         2590 t
ORIGIN      
        1 cttttaatga aagataaatt aaaaaaaatg tttgacagcg cttgacaaag gaaacggttt
       61 cattgtataa tgtaaacaca atgacaccga tgtcaaatat aaaaaagaag gtctttatat
      121 gaaaaactta acaaaaatta agttcaaaga gaatggggaa tttaatcatt tccctgggaa
      181 tacagttgta gcaaatcttt atactaaaca agatttgatg gaagttgttg atattattca
      241 atcacgttat agagaattac catttatcga taagtttact ttaactccaa ggaattctat
      301 tcacatgact gtaattgaat tgttgtgcca tgaaaatcgt gaaacggagt tttggagcag
      361 taatcttcct ctagatacac ctttacagga aatacatgat tactttgcta aacaacttga
      421 aatttttcca ttgttggatg aagaaattca tatgcgtata actgaaatgg gaaaacaaaa
      481 catactagtt gaacctgcag atgaagcttc tgcaaagaga ttagaagaaa ttcgtactta
      541 tgtctcagaa aaagcaggtg tttgtttccc taatcatgat agatatcaat tccatatttc
      601 aattgggtat cttcggattc ctctaaccga agaggaggaa gaagagttta ctaaagtcag
      661 agcagaatta actgaaattt tattagagaa gattccaact attactgtaa accgtattga
      721 ctatactgta tttgaagata tgagacaatt tgttccatat cacgaaaaat ttaaataaaa
      781 taataaaaaa ggagaactta tcatgaaaaa aatgaaagtt tggtctactg tacttgcaac
      841 gggagttgct cttactacac ttgctgcttg ctctggaggt tcaaattcta cgactgcttc
      901 ttcatctgaa gaaaaagctg ataaaagtca agaattagta atctattcga actcagtctc
      961 aaatggtcgt ggtgattggt taactgctaa agcaaaagaa gctggtttta atataaaaat
     1021 ggttgatatt gctggcgctc aattagcaga ccgtgttatt gctgagaaga ataatgcagt
     1081 tgcagatatg gtatttggaa ttggtgctgt tgactcaaat aaaattagag atcaaaaatt
     1141 actagtacag tacaagccta aatggttaga taaaattgat caatctttat cagataaaga
     1201 taattattat aatcctgtga ttgttcaacc attagtttta attggggcgc ctgatgtaaa
     1261 agaaatgcct aaagattgga ctgaattagg tagtaagtat aaaggtaaat attcaattta
     1321 tggtcttcaa ggtggtacag gacgggcaat tctagcaagt atcttagttc gttaccttga
     1381 tgaaaaagga gatttaggcg tatcagaaaa aggatgggaa gttgcaaaag aatactttgc
     1441 aaatgcctat actcttcaaa aaggtgaaag ctcagttgtt aaagcgttag ataaagaaag
     1501 cccaattcaa tacggtatga tctggggttc tggagcgtta atcggacaaa aagaacaaaa
     1561 tgttgaattc aaagttatga ctcctgagat tggtgtacca tttgtaactg aacaaactat
     1621 ggttttaagc actagtaaaa aacaagcgtt agctaaagaa tttattgatt ggtttggtca
     1681 atcagaaatt caagtagaat atagtaaaaa atttggatct attcctgcaa ataaagatgc
     1741 cctaaaagag ttacctgaag atacgaaaaa atttgttgat caagtgaaac cacaaaatat
     1801 tgactgggaa gctgttggaa agcatttgga tgaatgggta gaaaaagctg aattagaata
     1861 cgtaaaataa gaaataagag ttatttttta tgtccaagtc tctctattat ttctaaattt
     1921 aaggaagaaa taaagagagg cttttggttt agtaatagag ggagttagtg agatgattaa
     1981 atttgataat attcaaatta aatatggtga ttttgttgca attgataatc tgaatttaga
     2041 tatacatgaa ggggaattct ttacatttct tgggccttca ggatgtggta aatcaactac
     2101 tttgagagca ttggtaggtt ttttagatcc atcatcagga agtattgaag ttaatggaac
     2161 agatgtcact catttggaac ctgaaaagcg tggaattggt attgtatttc aatcttatgc
     2221 gctatttcca actatgactg tttttgataa tattgcattt ggtttaaaag ttaagaaggt
     2281 agctccagat gttattaaag ctaaagtatc agcagtggca gcaaaaatta agatctctga
     2341 tcaacagtta cagcgtaatg tatcagaatt atctgggggt caacaacaaa gggtagcatt
     2401 ggctcgtgct ctggttcttg aacctaaaat tctttgtcta gatgaaccat tgtcaaacct
     2461 tgacgcaaaa ttacgtgtag atttgagaaa agagttgaaa agacttcaaa aagagttagg
     2521 tattactact ttatatgtta ctcatgatca agaggaagcc ttgactttat ctgatagaat
     2581 tgcagtcttt aacaatggat acatcgaaca ggtcggtaca ccagtagaga tttatcataa
     2641 ttctcaaact gaatttgtat gtgattttat tggagatatt aatgttttga ccgatgaaac
     2701 agtccacgaa gtattactga aaaatacaag cgttttctta gaagataaaa aaggatacat
     2761 tcgattagag aaagttcgat tcaatcgtga aactgaacaa gatttcattc taaaagggac
     2821 aattattgat gttgagtttt ctggagttac aattcactat acaataaaag tttctgaaag
     2881 tcagattctt aatgtaacaa gtattgatag tcaggctgct attagatctg tcggagaaag
     2941 tgtggaatta tttatcacac catcagacgt tctgcaattt taaggaggtg cagtatgcgt
     3001 cataaattaa atttaaaaga ttggcttatt cgtttagggt taatctggtt cttagtaaca
     3061 tttattattt atccaaactt tgatctagta gtgaatgtat ttgtaaaagg aggagaattt
     3121 tcccttgatg ctgtacatcg tgttctaaaa tctcagaggg cacttcagag tattatgaac
     3181 agttttaagt tagcattttc actcattatt acagttaatg tcgtaggtat tctttgtgtt
     3241 ctatttacag agtactttga tattaaaggt gctaaaattt taaaattagg ttatatgacc
     3301 tctttaattt atggaggagt ggttttagcg actggatata aatttgtcta tggtccatat
     3361 ggattgatta caaaattttt acaaaatgtt atcccttctt tagaccctaa ctggtttatt
     3421 gggtatggtg cagtcttatt cattatgaca ttttcaggaa ctgctaatca tacattgttt
     3481 ttaacaaata caattcgaag cgttgactat cacactattg aggctgctcg aaatatggga
     3541 gcaaaaccat ttactgtttt ccgaaaagta gtgttaccaa ccttaattcc aactctattt
     3601 gcacttacta ttatggtttt tcttagtggt ttatctgcag tagcagcacc catgattgtt
     3661 ggtggtaaag aatttcaaac tataaatcca atgattatta catttgcagg gatggggaat
     3721 tctcgtgatt tagctgcctt acttgcaatt attttaggta ttgcaactac aattttgctt
     3781 actatcatga ataagataga aaaaggtgga aattatattt ctatctctaa gactaaagcg
     3841 cctcttaaaa aacaaaaaat tgcgtctaag ccttggaata tcattgctca cattgtagca
     3901 tatggattgt tcacagtttt catgcttcca ctaattttta tagtattata ctcatttaca
     3961 gatccagttg caattcaaac aggtaactta acattatcaa actttacttt agaaaattat
     4021 cgcttattct ttagtaatag tgcggcattc tctccattct tggtcagctt tatttattct
     4081 attattgctg cgacaacagc aacaattctc gcagttgtat ttgctcgtgt tgtcagaaaa
     4141 cataaatctc gttttgattt cttatttgaa tatggtgctc tacttccttg gttattacca
     4201 agtacgcttt tagcagtaag tttattattt acttttaatc agccacaatt tcttgtcttg
     4261 aatcagattt tggtaggtag tttggtaatt ctacttattg catatatagt tgtaaaaatc
     4321 ccattttctt atagaatggt acgtgctatt ttatttagtg ttgatgatga gatggaagat
     4381 gcagcaagaa gtatgggtgc ttcacctttt tatactatga tgaaggttat cattccattt
     4441 attttaccgg ttgttctctc tgttattgct ttaaacttta actctttatt aactgacttc
     4501 gacttatctg tattccttta ccatccccta gctcaaccat taggtattac gattcgatct
     4561 gcaggtgatg aaacagcaac atctaatgca caagctctgg tatttgttta tacaattgtt
     4621 ctgatgatta tttctggaac ggtattatac ttcacacaaa gaccggggcg taaagtaagg
     4681 aaataatcat gacagccact agtcttgggt tatcaaatat tgaaatagtt gtcaggattg
     4741 ttttatcagt agtcattggt agtataattg gtttagagag agggagcaaa tcccagcctg
     4801 caggcatccg aacttatagt attgtttgtc tagctgcatg tttgattatg atgacgaatg
     4861 aatacgtatc ttataaattt gggacaggag atcctacacg attaggagct caagttatat
     4921 caggtgtggg ttttctaggc gctggaacga ttcttattac agataaaaag aaaattacag
     4981 gtctgacaac tgcagcaggc atttgggctt cggcaggaat tggattagct attggagtag
     5041 gtttttatga gggagctctt ttagtagcca tttctgtttg gggtgtgata tccatgttcc
     5101 aaccactaaa aaaatatcta caaaatcgtt ctaaaatgat tgaattgtat atagtggtta
     5161 aatctacaga agcctataat cgtgtattgg tttattgtgc agaaaatggt atcagaatga
     5221 ccgattcaag aacagcgttt ggagatgtta attctgacag aattgaatac tttgatgttc
     5281 cagataaaaa aatagcgtca tttattactc tggaattatc aggtagattt gaacatcttc
     5341 gactgatgga agaaattgct aatattgtag gtgttattta tgtcgaagaa attagctgaa
     5401 atttagtaaa caaagggaca gatgtttttg tccttttgtt ttttagatgt tttaatcatc
     5461 taatttttga gtagattttt gctcagaggt ttttatgaaa gattataaaa tcaattttga
     5521 tttaggtaaa atagaatatt ttgataataa ttgtttgata caagtataca aatttatttc
     5581 tttttatgat atttgtgaaa tggtgtttgc ctttcattta cctcctgatg agttgataac
     5641 aaatgttatc tttaaagaaa aaattaactc tatgttaaaa tgttacattg acaggctatt
     5701 atatgttttt ataaatccta cacattttac tgaaaaagtt aatctacaat tttatggcag
     5761 ttttttctca tatgaattta tttgtcgtga agttggaaac attcttaaaa ataaaggtgt
     5821 gaaatgcaat ttaaattttt ttgaagggga agaatatttg taaaattaat tttgtgaatt
     5881 tgtggatctt aaataattta ctaaattctc ttgttaaaaa aaattaaata ttgtatagta
     5941 atattagaat gttcgtaatg gaggactgaa atgaaaaata taaatgggaa aaaggtaact
     6001 atttatgata ttgcacgttt atcaggtttt tctccaaaaa cagtttcacg agttattaat
     6061 ggtggagtaa atgttaaaga agaaacttat caagcgattc aaaaagttat agaggaattg
     6121 tcgtatattc caaatgcgta tgcaaaaaat ttaactaaaa aagaagctat aaatatattg
     6181 atttctgtaa aaaaaatcga ctcttttcct ttaatttggt ttcatacgct gttagataaa
     6241 gtgttacgga cgtgtaaaga atttggtgtt aatgctatag ttgagtactt tggagaggag
     6301 gatacaatta gtaattcaat tatatcaagc acaggtagcc tagtagatgg tgttattgtt
     6361 ttttatgaaa gtgtagatga tatcagaatt cagtatttaa agaagaacca tatgcctttt
     6421 cttgttttcg gtgaatctca aacgtctgga gtagtctatg tatctaataa taattttcaa
     6481 gctacttatg atatgatgaa agctgtaact gaagaaaaat ttaaaaatat gtggttgctt
     6541 atgggaggag aatcccatgt taataaggat cgtgaaagag gtgtgcgttc ttttctgaat
     6601 gataaaaatt actttatgga tttaaaagtc atttatggct tatctacaat tgattcagtt
     6661 tattcttatg caatgcagca cttgactacc caaaattatc cggacattat ttttgtttca
     6721 ggagatgaaa aggttcaggg cttgattcgt gcgtgctatg agaaaggaat tcttattcca
     6781 gatgatattt ctatcattgg atttgataat attcctattt ctcagtatta tactccagct
     6841 ttgtctacaa ttgctcccaa ttatgttaaa ttggctaaag aaatgataga aggtgtttta
     6901 gcaattatta aaggggaaag tgtcacatct gttgaagttt ctcctaaatt tgttaggaga
     6961 cagagtttct agtattgttt aattcttgaa aatcctagtg gaaaagatgg agtgttctaa
     7021 aataagttct gtttagcctc cattcagttt ctattgagtt ggatccaatt tctaacaagt
     7081 gggacatgtt catgttcgat ctaaggaaga tatggtcaca ttcgaatgat atcaaaaaaa
     7141 tcgtaaaaaa gtcttgacaa agaaaaaaca aagtattaaa ataagttcaa caaatcagaa
     7201 aagtaactat ttttgatctt cagggagcct gtggtgattg tgaataggtg gttggaagta
     7261 gtgaaagtgg gctgattttg aaaatgaatt tgaaacaatg aaaattcggt gcgcacacct
     7321 tacagtgcag cttgttgtta aacaaggcag agatgtaaag ggatagtccc tttataattg
     7381 aggtggcacc gcgttaccaa cgccctcaca cggaagtata ttctgtgtgt gggctttttt
     7441 ctatccgtcg tttggtttat cttttattag ggccttaagt cgctttgatg aacttgagtt
     7501 ctatctacag ctccttgcct cctactaaaa gtaaacaaaa aggctggact aatacgaaaa
     7561 aagcta
//
LOCUS       DAVBHS010000097         7521 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVBHS010000097 DAVBHS010000000
VERSION     DAVBHS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7521
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.097"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            176..493
                     /locus_tag="VQ651_001513"
     CDS             176..493
                     /locus_tag="VQ651_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HET2754488.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMYGRETILLPEEN
                     DLF"
     gene            complement(538..1350)
                     /gene="folK"
                     /locus_tag="VQ651_001514"
     CDS             complement(538..1350)
                     /gene="folK"
                     /locus_tag="VQ651_001514"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET2754489.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFEFYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPTLKQPIRNLYDALKK"
     gene            complement(1393..1947)
                     /gene="folE"
                     /locus_tag="VQ651_001515"
     CDS             complement(1393..1947)
                     /gene="folE"
                     /locus_tag="VQ651_001515"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET2754490.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(1928..3250)
                     /locus_tag="VQ651_001516"
     CDS             complement(1928..3250)
                     /locus_tag="VQ651_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET2754491.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGIPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(3252..4226)
                     /gene="folP"
                     /locus_tag="VQ651_001517"
     CDS             complement(3252..4226)
                     /gene="folP"
                     /locus_tag="VQ651_001517"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET2754492.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGITPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(4321..5028)
                     /locus_tag="VQ651_001518"
     CDS             complement(4321..5028)
                     /locus_tag="VQ651_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2754493.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(5081..6499)
                     /locus_tag="VQ651_001519"
     CDS             complement(5081..6499)
                     /locus_tag="VQ651_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET2754494.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            6689..7501
                     /locus_tag="VQ651_001520"
     CDS             6689..7501
                     /locus_tag="VQ651_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2754495.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
BASE COUNT         2254 a         1758 c         1408 g         2101 t
ORIGIN      
        1 aactgaaagt tatataatca agttaatttg ggaatttacc tattaacttc ggtagatttt
       61 tgggaatttt tttccttatt tttccctgta gttaaaatcc ttttcaaaaa atcacctttt
      121 cttgattttt aatcctattt gctgtatgat aagggaaaag aaaggggaca gagatatggc
      181 ttttaccaat acccacatgc ggtctgctag ttttggcatt gttaccagct tgcccgatga
      241 catcattgac tctttttggt atatcatcga tcatttctta aaaaatgtct ttgaattgga
      301 agaagaactc gagtttcaat tgcttaataa ccaaggaaag attaccttcc acttttcaag
      361 tcaacacctc cctacagcca ttgattttga ctttaaccat cctttcgacc ctcgttatcc
      421 cccaagagta ctggttttag acatgtacgg tagagaaact atcctcctcc cagaagaaaa
      481 tgacctattt taaaaactct agccttcagt tgcaagtaac tgaaaactag agtttttcta
      541 ttttttcaaa gcatcataca agttgcggat cggttgtttt aatgtcggat ggataaaatg
      601 aggcgcaatt tcctgtaagg actcaaggac aaaaaggcgt tccgctatgt aaggatgagg
      661 caatatgagg tcgtctgtat aaaggatctg gtcctccaca aagagcaagt ccaaatcaat
      721 caaacgaggt ccccaatgca cttctctcac ccgtcccagc tctgactcaa tggctaacaa
      781 ggtttctaac aagtcttgtg ctggtagcca ggtttccacc tcaaccactt gattggcaaa
      841 gctatcctgc tccactccac cccaaggctc cgtcgctaag acactggact ctttgagaat
      901 atagatgcca cgagctcgca gtttgtcaat ggcttgcttc aagtttgctt gtttatctcc
      961 catattgctt cctagggcga taaaggctcg ttgcttgcgg cgatgaatgg ttaccgagca
     1021 agtatctagt gacaaatgca ccggtgccca aggttttttc agttccaact tcatttcttg
     1081 gacaagagga taaaactcaa aggtacgttc caccagttta taggctaccg tttcaatcaa
     1141 atcttcactc gtttcctgaa accaagtcgt ccactgctga cataattctc cgtaatggac
     1201 agaggctgtt aaatccaagt ctgtagctgc cttggtcata tcataggata ggatggctga
     1261 aacgacaaat ttctgcccca attctttctc actaggaaaa agaccatgat aggcaaacat
     1321 ttccaaatcc ttaatctgca gttgatccat aatgattcct ttctagaaaa atccgcttga
     1381 agcggattct ttttatagcc ccattaaacg ataagcttgg tcgcggaggt ccttatctgt
     1441 ttcaaataga ccacgagcta ctgtcgtcaa ggttgcagtg cctggttttc taacaccacg
     1501 catactcata cacatatgtt ccgcctcaat gacaacaaag gctcctttag cacctagata
     1561 gtccatcaag gcatcggcca cttcgatatt caaacgttct tgaatttgtg gttttttcga
     1621 ataaacttca accgtacggg ctagcttaga caagcctgcc acacgaccat ctggaatgta
     1681 ggcaatgtgc gctctaccat aaaatggcaa gaagtggtgt tcacacatgg tatggaaaaa
     1741 gatatccttt tctaccacca tattatcgtc aataatttca aaggattttg acaaatgttc
     1801 ctctgctgtt tgaccaagac ctgaaaaaat ctcttgatac atacgggcta cacgagcagg
     1861 tgtttcctgc aagccctcgc gattagcgtt ctctcctaca gcctcgataa tcatttttac
     1921 agctgcttca atcttttgtg tatccattct cgttcttcct ctccatcaga taggccctca
     1981 cttggctcaa gaaatacaag gaacctgtga caatcctaac tgtttgtttc tcttcttttt
     2041 tatctgtcaa attctgctct agaaaatcat gccaatcttg gtagctgaga tttctagact
     2101 tagctgcctc tttcagcacg ctttcatccg tcgcccgact atccgcaaaa tgtgttagag
     2161 taagctcggt atctggcatg gctcccagca agtccaacat atcctccaag gctttggttt
     2221 tgatacaagt gaagaggatt tccttatgat aatccgcaaa acgttcttgc aaggttacca
     2281 acaaggcctt gatagcatgg ggattatggg ctccatccaa aatcatcaag ggatctcttg
     2341 acacgatttc caaacgccct ggccaacttg tttcttccaa ggcttgacca agaaagtcat
     2401 tgcttgctag ctctcgacca tcttcttgac aaaaagtatc aagtaaagct atggccatcc
     2461 cagcattctc tatttggtac aaaccaagca ggctagtctg gaagcgacct tgtctgacag
     2521 cacttgtata gtcaaagact tcccctgtca ccacactttc ttgatgacga acctgataat
     2581 ctgtcccgta ggcaagtctc ggcgcatctt tcccttccgc aatgcggtca atcacagtca
     2641 aggcttctgg agcaatacgc cctgttacca agggtatccc ttgtttgata atacctgcct
     2701 tctgctctgc tatggcctcc aaggtgtcac caagtagggc cacatgatcc aagccaatag
     2761 ttgtaattcc tgtcaaaatg ggctgacaga cattggtact atccaaaagt ccacccatgc
     2821 caacttccat gatggccaca tctacttgct ctgaggcaaa gtagtcatag gccagggctg
     2881 tgataatctc aaactcggtt gtgccctgta aattggcgac cgcttctccc tccagcaaag
     2941 actgatagtc tgccatgaga gcttctagcc tcgcttctga gatcgattcc ccattgatgc
     3001 taatctggtc tgtgtaatga atgagatagg gcgagctaaa cacgccaact ctcagcccta
     3061 gcttttctag catctttttc aaaaaagcaa tagtcgagcc cttgccgtta gtccctccga
     3121 tatggaggac cttgagtttg agatggggat tgccacgcaa agctaacagt tccaccattc
     3181 gttccaatcc aaaatgcggt tgatccgtcc ggtagttagc aatccactga ttgttttcaa
     3241 tttctttcat cttatttata ttgttttaaa tctaaatttt ccgcttcatc agccagacga
     3301 atggcagagg caatttcaac tgccatcctg tgactagcta cgtcatgcac gcgcaccact
     3361 tctacaccct gtctcgcagc gatactagtt acatgagccg aagccgtgtc ccgatttcgg
     3421 aaaccaagct ctgtctcagg attgacttca aacccacttt cttcaaggat attgatgaca
     3481 aatcgcttgc gcgacactcc gagaaagatt ggatagccct tctgatgtag tttatccagg
     3541 tcccgtaaaa ggaggagatt ttctttcttg gtcagaccaa agccaattcc tggatccaac
     3601 aggatatttt ctggtgtaat accagcttct gccgctctcg ctagtgctcg ttcaaagaaa
     3661 gccaccatca agtcttcgat tggcaatgtt tcaaagtcag ctaactcttt ttctgtaaag
     3721 gtttgaccaa agccaaaatg agggaagata agcgaactag gatgttgagg tcgagccatg
     3781 actggattaa acatgatgac tactttcgct ccagctttag caaccacatg ggccattttt
     3841 tcatcgccca tgagaccagt gatatcatta actagattgg caccagcagc caaagcagcc
     3901 tctgccacct gacttttcca agtatcgata gaaataagga catcactttc cttgcgaatc
     3961 gctttgatca ctggaacaac acgctggatt tcctcttcta tctcaacata gctactgccc
     4021 ggccgagtcg attctccgcc gatatcgagc atactggctc cttctgctat caatttacga
     4081 gcctgctgga gtgcctgctc aagagcaaaa aattgaccac cgtccgaaaa ggagtctggg
     4141 gttacattga taattccgca aataactgtc tttgcatgat tggctttact tgacatatcg
     4201 gtcactccct caaggatttt catcatatta tttttctatt ttaccataaa aagaaaaaga
     4261 tggacacgat tgcattcatc tttccctagt agaaagaggt cggttcctac ataaaaactt
     4321 ttacacagaa attcctaatg tccgactcat aatcaccaca agagccaaca aacagaaagc
     4381 aatcccatta acaatcatgt gaagcaagat cgacatttcc aaacgttggg tcttgtaggc
     4441 cgtccaagat agaactgtcg acatacctcc ataaatcaat aaagaaggta aattacttgg
     4501 ttgatgcaat aaagcaaaca caatcgtacc gactacaaat cccaagttct ccttgcctcg
     4561 gaaaatcttt ttaggaacaa tcccacggca caagatttcc tcacaaattg gagcaagcaa
     4621 ggctagcaag aagaagctag aaatcagaga actattctga accatatcgt taatctgaga
     4681 ctggttagct gttgtcgtct catttgacag ttgcaacaag atagaaccaa gtatatttga
     4741 cccgacaata actaaataac tcaagcccaa acgtgccaaa tctttagctc taaaaaaaga
     4801 aaaattaaaa ctagctaact tggttttacg agctcccata ataaatagag ccagaaccac
     4861 aattgaaagg ccagtaacta tcagccctga ctgtagcagg gctacttctt ttaaagtcaa
     4921 aatagaggta accactaggg gaacctgata taaaaggacg gcaagtaaaa agaccaaaat
     4981 ccagcctgca cgattcaaca attctttcca catatttttc tctttcatca cctatctcct
     5041 ttgtattaaa aaagggggga aatccccctg ggtcattcta ttataaggcc atgctgatgt
     5101 agttaaggat aaacaaggca tccaaaatcc aaatcatggc atgaacatct ttaacttgac
     5161 ctttgacaag cttagtcaaa gtgtaagtca agaaaccaac tgcaatccct tgagtgatag
     5221 agtagctgaa tcccataaag atagatgtga agaaggcagg aactgcttca gacatatcat
     5281 cccaatggat atttttcaag ctaccaagca tcataatccc aacgataatc aagattggag
     5341 ctgtagccgc tgttggtacg atcgctagaa gtgggctaaa gaagcttgaa atcgcaaaac
     5401 agatagctac aaccaaggct gtcaaaccag tacgtccacc tgcaccgata ccagcagcag
     5461 actcaacata agtcgttacg tttgaagtac ctgcaatggc accgactgtc gttccaatca
     5521 aatcagagta aagagcctta tccaatttgg ctgattggtg attttcacca tttgtcgcta
     5581 caataccaac tttttcacct gtaccgatca aggtaccaat tgtgtcaaaa atatctgtca
     5641 atgagaaggc aagaatggcc atcagagttt caggcaagcg agctgtatct gaaaccaaag
     5701 ctcccaaacc ttctgaacca agagctgcac caaagattgt cttcaaatct tcaaaagctg
     5761 caccaacatg gttattagca aaatcgatac tagacaaatc aaccaaacca actgcaatag
     5821 caagaacagt tgttgtcaag atagagagaa taattccccc tttaatccct ttgatgacaa
     5881 agaagatagt aatggcaagt cctgcaagag ccactaaaac agctggatta ttaaagctga
     5941 ccaatcctgg aactgctgaa gagtttgctg caatcgttgc ttgagctttg tcagcccctt
     6001 ctcctacaac agtatagttg cctggatcaa tcgtgaattt caaaagtcca gcattcttaa
     6061 tccctacata ggcaaggaag acaccgatac cagctgaaat agctgagcga agagcattgg
     6121 gaatcgattc aatgatcatt ttacgaacat ttgtcaaggt aataatcaat gaaataatcc
     6181 cacagatgaa gaccatagct agggcttctt gccaagaata accaagcccg aatacaactg
     6241 taaaggtaaa gaaggcattg agtcccatac ctggtgcttg ggcataaggt aagttagcat
     6301 aaaaagccat catcaaggta cccgctactg caccaataat cgtcgctagg aagacgccct
     6361 gagcaggcat tcctgtttgt gaaagtattt gtgggtttac aaagagaata tagctcattg
     6421 caaagaaagt tgttaaacca gcgagaacct ctgtacgaac gtctgtaccg ttctctttta
     6481 gtttaaataa tttgtccatc atcaatctcc ttttaatttt tacgaacaat aatagaatta
     6541 tatcatttat cattcacttt ttcaatgtct ttgtttgatt tgtttaagaa attaatccaa
     6601 tttcattttt tgtttggaat ctgcttttct gcctgctttc tgttataata gaagacatct
     6661 tatctgaaaa cacactagaa aggtccatat gacgaaaaaa attattgcag ttgacctgga
     6721 tggaaccctg ctcaactcag acagtcaaat ttctgacttt accaaaagaa ccatcaaaaa
     6781 agttgctgaa aaaggccatc aggttattat tacgacaggt cgcccttacc gtatgtcaaa
     6841 agatttttac cgtgaactgg gcttagacac tcctatgatt aacttcaacg gatcccttac
     6901 tcatttacca gaccaagttt gggattttga aaagtgtttg actgtagaca aaaaatatct
     6961 gctagatatg gttcaacgtt cagaggacat tcaagccgat tttatcgctg gagaatatcg
     7021 taaaaaattc tacattacaa atcccaatga agaaattgcc aatcccaaac tatttggtgt
     7081 agaagctttc cagcctgaag atcaattcca gcctgaattg gtgaccaagg accctaactg
     7141 tatcctcttg cagaccagag ccagtgacaa atatgccttg gcaaaagaaa tgaacgcctt
     7201 ctaccagcat caactttcta tcaatacctg gggaggtccg ctcaatatcc ttgaatgtac
     7261 cccaaaaggt gtcaacaagg cctttgcttt ggactacttg ctcaagataa tgaatcgtga
     7321 caaaaaagat ttgattgcct ttggagatga acacaatgat accgaaatgc tcgcttttgc
     7381 tgggaagggt tatgccatga aaaatgccaa tccagagcta ctcccttatg cagatgagca
     7441 aatttccctt accaacgacc aagatggggt tgccaaaacc ctacaagact tattcttata
     7501 acctatactg atactcaatg a
//
LOCUS       DAVBHS010000098         7490 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVBHS010000098 DAVBHS010000000
VERSION     DAVBHS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.098"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..1039
                     /locus_tag="VQ651_001521"
     CDS             <1..1039
                     /locus_tag="VQ651_001521"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit I is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HET2754496.1"
                     /translation="IKVMDERFGHSIYIYESETLSDNQDEIPIKLTNHSLIEPFELLT
                     EMYALPKYYEKDPTPVLAPFYFTFFGMMVADLGYGLLLFLGTMLALKIFHLPSETKRF
                     LKFFNILGVAVAIWGGIYGSFFGYELPFHLISTTSDVMIILVVSVVFGFITVFAGLLA
                     SGLQKVRMKKYAEAYNSGFVWCVILLGLLFIAVGMLMPDMRLLFVLGKWVSIFNAVGI
                     LVVSIIQAKSLSGIGAGLFNLYNISSYIGDLVSFTRLMALGLSGASIASAFNLIVGLF
                     PGTLAKLTIGLVLFILLHAINIFLSLLSGYVHGARLIFVEFFGKFYEGGGKPFQPLKA
                     SEKYIKVITKN"
     gene            1055..1531
                     /locus_tag="VQ651_001522"
     CDS             1055..1531
                     /locus_tag="VQ651_001522"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HET2754497.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            1565..2146
                     /locus_tag="VQ651_001523"
     CDS             1565..2146
                     /locus_tag="VQ651_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754498.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSVIVKK
                     KEVEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALIEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNHLFSEQPISNESGLLI
                     SIGKIDDNYLYKTLIGSISKEESSSIANQIFIN"
     gene            2211..3218
                     /locus_tag="VQ651_001524"
     CDS             2211..3218
                     /locus_tag="VQ651_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HET2754499.1"
                     /translation="MDTNLFSKINTTISVKENDFITEDKFQKIIQSKDTETMAFILEA
                     TPYHLSIDVLENPSQTETSLMTKLVNDYRWAYAESPSNKIVTLFALRYVYHNIKVLLK
                     SKAAIKKDFSKLLIPIGIFDIESLKHLVSSLHSDTLPDFMVHEVESIWNEYETFNNIR
                     VLDVGADLAYFKHLKLLSNELDEVLSQVIVAMIDFYNIITVKRGLSQNKSHGDILQLL
                     SDEGSISAKEFIYIVENQEIFVWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDL
                     LLYKILDQGRYNVDGPLVLARYLLGCEFEVKNLRMIISALQNTIPFGSIKERIRPHYG
                     S"
     gene            3208..3528
                     /locus_tag="VQ651_001525"
     CDS             3208..3528
                     /locus_tag="VQ651_001525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HET2754500.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            3591..5366
                     /locus_tag="VQ651_001526"
     CDS             3591..5366
                     /locus_tag="VQ651_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HET2754501.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFSNVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGIPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGIYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            5367..6752
                     /locus_tag="VQ651_001527"
     CDS             5367..6752
                     /locus_tag="VQ651_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HET2754502.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLSSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            6775..7386
                     /locus_tag="VQ651_001528"
     CDS             6775..7386
                     /locus_tag="VQ651_001528"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HET2754503.1"
                     /translation="MVRLNVKPTRMELNNLKERLKTAERGHKLLKDKRDELMRRFISL
                     IRENNRLRKEVESYLIDNLKSFAVAKSLKNSLMVEELFSIPSKEIELFIEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIDKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
BASE COUNT         2458 a         1042 c         1525 g         2465 t
ORIGIN      
        1 tataaaagtt atggatgagc gttttggaca ttctatttat atttatgaat cggaaacatt
       61 gtcggataat caagatgaaa tacctatcaa attaacgaat cattctttaa ttgaaccatt
      121 tgaattattg acagaaatgt atgctctgcc caaatattat gagaaagatc ctacacctgt
      181 attagcacca ttttacttta cattttttgg aatgatggtt gctgatttag gctatggttt
      241 actattgttt ttaggaacaa tgttagcatt aaaaattttt catctacctt cagaaactaa
      301 gagattttta aaattcttta atatattagg ggtagccgtt gcaatttggg gtggaatcta
      361 tggctcattt tttggatatg agttgccatt tcatctgata tctacaacct ctgatgtcat
      421 gattatatta gtagtgtcag ttgtgtttgg gtttattaca gtatttgcag gtttgttagc
      481 ttcaggacta caaaaagtaa gaatgaagaa atatgcagaa gcatataatt caggatttgt
      541 gtggtgtgtt attctgcttg gcttgttatt tattgctgtc ggaatgttga tgcctgatat
      601 gagactgtta tttgtattag ggaaatgggt atctattttt aatgctgtgg gaattttggt
      661 tgtttctatt attcaagcca aaagcttgtc aggtattgga gcaggattgt ttaatctata
      721 taacatttca tcttatatag gtgatttagt tagtttcact cgattgatgg cattaggatt
      781 atctggagca agtatagcat cagctttcaa tttaattgtt ggtttgtttc cgggaacatt
      841 ggctaaacta acaattggat tagtattatt cattctttta catgcgatca atatttttct
      901 atcgttacta tcaggatatg ttcatggagc acgtctgata tttgttgaat tttttggtaa
      961 gttttatgag ggtggaggaa aaccatttca acctttgaag gcttctgaga aatatattaa
     1021 ggttattaca aagaattaat ggaggatata tataatggaa catttagcaa cttatttttc
     1081 aacctatgga ggagctttct tcgctgcatt gggaattgta ttggcggttg gattaagtgg
     1141 tatggggtct gcttatggag ttggtaaggc tgggcaatct gccgcagctt tactgaaaga
     1201 acagccagaa aagtttgcct ctgctttgat attgcaatta ctgcccggaa cacaaggatt
     1261 atatggtttt gttattggaa ttttgatttg gttgcaatta actccagaac ttcctttaga
     1321 aaaaggcgtt gcttatttct ttgtagctct tccaattgct attgtaggat acttttcggc
     1381 taagcatcaa ggaaatgtag cagtagcggg aatgcaaatc ttggctaaaa gaccaaaaga
     1441 attcatgaag ggagcaattt tggctgccat ggtagaaacc tatgcaattc ttgcctttgt
     1501 cgtatcattc attttgaccc ttcgtgtata agaaattaat ttgctattca aaggaggtgt
     1561 ctaaatgagc aatttagaaa acttacgaga gtctgttatt gaacaagctc atgaaaaagg
     1621 gcgtatgaaa ttattggatt ccaaaaagaa gattgatgat gaatttgaaa tgcaaaagtc
     1681 ggtcattgta aagaaaaaag aagtggaaca tgaacgaaag ttaaaagaat tacaacagaa
     1741 atatcaaata atttttcaac aattaaaaaa taaggaacgc caatcaacgt tagtatcaaa
     1801 acagaaaata ttaaaagaac tttttcaatc tgctttaata gaaatggaat cttggagtgc
     1861 agataaagaa atggagttca tctatcgaat tctggaacga tattcacaac aagaggtcat
     1921 agtaactttt ggggagcgga ctttagctaa attcaatctg gaacaattag agaaattgaa
     1981 attctctttt ccaaatcatt tatttagtga acagcctatc tcaaatgaat caggcttact
     2041 tatttcaata ggtaaaattg atgataacta tttgtataaa acattaattg gatcgatttc
     2101 taaggaagaa agttcaagta tcgcaaatca aatttttatc aattaaggat gaaattggtt
     2161 aatccttctt agaaatttgg agtattccaa taaaattaga aaggtatttt atggatacta
     2221 atcttttttc aaaaataaat acgacgattt cggtaaaaga aaacgatttt attacagaag
     2281 ataaatttca aaaaattata caatccaaag atacggagac aatggcattt atcttagaag
     2341 caactcccta tcatttatcg attgacgttt tagaaaatcc tagtcagaca gagacttcgc
     2401 taatgacaaa attagtcaat gattatagat gggcctatgc tgaaagtccg tctaataaaa
     2461 ttgtgacttt atttgcttta cgatatgttt atcataatat caaagtttta ttaaaatcta
     2521 aggcggcaat taagaaagat ttttctaaat tattaattcc aatagggatt tttgatatag
     2581 aaagtttaaa acatttagtt tcttccttac attcagatac acttcctgat tttatggttc
     2641 atgaagtaga atcaatttgg aatgagtatg aaacttttaa taatattcgt gtacttgatg
     2701 tcggagctga tctagcatat tttaaacatc tgaaactttt atctaatgag ttagatgagg
     2761 tgctgtctca ggttattgtc gcaatgattg acttttataa tattattact gtaaaacgtg
     2821 gtttatctca aaataagagt catggggata ttttacaatt actttcagat gaaggaagta
     2881 tttctgctaa agaatttata tacattgtag aaaatcaaga aatatttgtg tggttcaata
     2941 aaataaatcc aagcttagat tcaatctttt caacttatga attgaagatg caggacgcaa
     3001 caatttcatc ttctgagtta gaatttttat gtgatttatt attgtataaa attttagatc
     3061 aaggaaggta caatgtagat gggccgttag tacttgctag atatttattg ggatgtgagt
     3121 ttgaagtaaa gaatctcaga atgattatat cagctcttca aaatacaatt ccctttggat
     3181 caataaaaga aaggatacgc ccgcattatg gaagctaata agtataaaat tggcataatt
     3241 ggtagccgtg atattatttt accctttagc atgattgggt ttgatatatt tcctgcctat
     3301 caagaacaag aagctataaa tactctaaga aaattagccc aatctgatta tggtgtcatt
     3361 tatatcactg aagacattgc ttcaatgata ttagatacaa ttcgccatta tgattctcaa
     3421 gttgtgcctg ctattatttt attaccgact cataaacaag gtttaaattt aggattaaaa
     3481 cgtatagagg ataatgtgga gaaagcagta ggacacaata ttttataata atgtacaaaa
     3541 ttgtctgtaa tattattcta taatttttgg acttagtaag gagaataact ttgactcaag
     3601 ggaagattat aaaagtatcg ggacctctag ttattgcatc aggtatgcag gaggctaata
     3661 ttcaagatat ttgccgtgta ggtaagctag ggttaatcgg tgaaattatt gaaatgagaa
     3721 gagatcaggc atctatccaa gtctatgagg aaacatctgg tcttggtccg ggagaacctg
     3781 ttgttacaac tggagaacct ctctcggttg aattaggacc aggattgatt tctcaaatgt
     3841 ttgatggcat acaacgccca ttagatcgat ttaaattggc tactcataat gattttctag
     3901 ttcgtggggt agaagttcca agtttggata gagatattaa gtggcatttt gattccacta
     3961 tagcaattgg tcaaaaagtg agtacgggtg atattctagg aactgtcaag gaaaccgagg
     4021 tagttaatca taaaattatg gttccttatg gagtatctgg cgaagtcgtt tctatcgcat
     4081 ctggcgattt tacaattgat gaagttgtat atgaaataaa aaaattggac ggtagtttct
     4141 ataaaggaac gcttatgcaa aaatggcctg ttcgcaaggc gcgtcctgtt tctaaacgtt
     4201 taatcccaga agaaccatta atcacaggtc aacgagttat tgatacattc tttccagtaa
     4261 ccaaaggggg agcagcagct gttcctggac cgtttggagc aggaaagaca gttgtacaac
     4321 accaagtagc taaattttcc aatgttgata ttgttattta tgtcggttgt ggagaacgtg
     4381 gaaatgaaat gacggatgta ctgaatgagt ttcctgagtt gattgaccct aataccggac
     4441 aatcaattat gcaacggaca gttctgattg ctaatacttc aaatatgcct gttgctgctc
     4501 gtgaggcttc aatttataca ggaattacca tggctgagta ttttcgtgat atgggctact
     4561 ctgtcgccat tatggctgat tcgacttcac gttgggcaga agcactacgt gaaatgtcag
     4621 gacgtctaga agaaatgcct ggtgatgagg gttatcctgc ttatctggga agtcgtatcg
     4681 ctgaatatta tgaaagagcg ggacgttctc aggttctagg gattccagaa cgtgaaggaa
     4741 ctattactgc tattggagct gtatcgccac ccggtggaga tatttcagaa ccagttactc
     4801 aaaacacttt acggattgtg aaagtttttt gggggcttga tgctccgttg gcacagagac
     4861 gtcattttcc tgcaattaac tggcttacat cttattcact ctataaagac agtgtgggaa
     4921 tttatataga tggtaaagag aagacagatt ggaatagtaa aataactcgt gcgatgaact
     4981 acttacaacg ggaatctagt ttagaggaaa ttgttcgtct tgttggaatt gattctctgt
     5041 ctgataatga acgactaacg atggaaattg ctaaacaaat tcgagaagat tatttgcaac
     5101 agaatgcttt tgattcagta gatacattca cttcgtttgc aaaacaagaa gcaatgctaa
     5161 gtaatatcct aacttttgct gatcaggcaa atcatgcttt agagttaggt tcttacttta
     5221 cagagattat ggaaggtacc gtggcagttc gagaccgtat ggcgcgaagt aaatatgttt
     5281 cagaagatag attagatgaa atcaaaatta tttcaaatga gattacacat caaattcatt
     5341 tgatattaga aacaggaggt ctataaatga gtgttataaa agaatacaga actgctagtg
     5401 aagttgttgg gcctcttatg attgttgaac aagtaaataa tgtgtcttac aatgagttag
     5461 ttgaaattca acttcataat ggagaaattc gtcgtggaca agttttagag attcacgaag
     5521 ataaagcaat ggttcaactt tttgaaggat ctagtggaat aaatttagaa aagtctaaaa
     5581 ttcgttttgc tggtcatgca ctagaattgg ctgtatctga ggatatggtt ggtcgtattt
     5641 ttaatgggat gggaaaacca attgatggtg gaccagattt aattccagag aaatatttag
     5701 atattgatgg tcaagctatt aatcctgtat ctagagatta tccagatgaa tttattcaga
     5761 cagggatctc ctctattgat catttgaata ctcttgtacg tggtcaaaaa ttaccagtat
     5821 tttcaggttc gggcttacct cataatgaat tagcagctca gatagcaaga caagcgactg
     5881 ttttaaattc tgatgaaaat tttgcggttg tatttgcagc aatgggtatt acttttgaag
     5941 aagctgagtt ttttatggaa gaactcagaa aaacaggagc gatcgatcgt tcagtcttat
     6001 ttatgaactt ggcaaatgat cctgcaattg agcgtattgc aactccccgc attgctttaa
     6061 ccgcggcaga gtatctagct tttgaaaaag atatgcacgt tctagttatc atgacggata
     6121 tgactaacta ttgtgaagcg ttacgtgaag tctcggcagc gcgccgtgaa gttccaggta
     6181 gacgaggcta tccgggatat ttatatacaa atttatcaac tctatatgaa agggctggtc
     6241 gcttagttgg taaaaaaggt tcagtgacac agattcctat tttaacaatg ccagaagatg
     6301 acataacaca tccaattcct gatttaactg gatacattac tgaagggcaa attattttgt
     6361 ctcatgagtt gtataatcaa ggttatcgtc caccaatcaa tgttttatct tctctctctc
     6421 gattaaaaga taagggatct ggagaaggta aaactcgtgg agatcatgct ccaactatga
     6481 atcaactgtt tgcagcctat gcccaaggga aaaaggttga agagttagca gtagtattag
     6541 gagaatcggc tttatctgat gtagataaat tgtatgtgag atttacaaag cgttttgaag
     6601 aagagtacat caaccaagga ttttataaaa atcgaaatat agaagatacg ttgaatcttg
     6661 ggtgggaatt actatcaatt cttcctagaa cagagttaaa acgtatcaaa gatgatttgc
     6721 ttgataaata tttacctttg gtagaagttt aatccagaaa tggagtgatt atctatggta
     6781 cgtttgaatg taaaaccaac tcgtatggaa ttgaataact taaaggaacg tttgaaaaca
     6841 gctgaacgtg gacataagtt attaaaggat aaaagagatg aattgatgag gcgatttatt
     6901 tctttgattc gtgagaataa tcgacttcgg aaagaagtag aaagttatct aattgataat
     6961 ctaaaatctt ttgcagttgc taaatcatta aagaactctc taatggtgga ggaattattt
     7021 tcaattccat cgaaagaaat tgaattattt attgagaaag aaaatatcat gagtgtaaca
     7081 gttcctagaa tgcatatgaa tattacttct caaaatgaga acagtgaata cagctattta
     7141 tcttctaata gtgaaatgga tgatgtattt gctacaatga atagtttaat tgataaatta
     7201 ctaagactgg cagaagttga aaaaacgtgt cagttaatgg ctgatgaaat agaaaaaaca
     7261 cgtagacgtg taaatggttt agaatattcg attattccaa acttgtcgga aactattcat
     7321 tatatagaat tgaaactaga ggaggcagaa agagccaatt tagttcgtat tatgaaagtg
     7381 aagtagttcc tttatttaga tttttaatta gatgaacaaa tatcagcttg gataaggcgg
     7441 taagcctttc taagcttttt taggctcttt gtcaactgta gtgggttgaa
//
LOCUS       DAVBHS010000099         7441 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVBHS010000099 DAVBHS010000000
VERSION     DAVBHS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7441
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.099"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            122..277
                     /locus_tag="VQ651_001529"
                     /pseudo
     CDS             122..277
                     /locus_tag="VQ651_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            477..3752
                     /gene="rexB"
                     /locus_tag="VQ651_001530"
     CDS             477..3752
                     /gene="rexB"
                     /locus_tag="VQ651_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HET2754504.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNRERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPISPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            3749..7399
                     /gene="addA"
                     /locus_tag="VQ651_001531"
     CDS             3749..7399
                     /gene="addA"
                     /locus_tag="VQ651_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HET2754505.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEICSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FAKLGQLSDQITILDYQERYHQDTWKLAKTFQTFMSHFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALVEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIDEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQIIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
BASE COUNT         2270 a         1573 c         1585 g         2013 t
ORIGIN      
        1 tagattgaaa taagatacga acagcttgat tagggaaatc aaattaattt ctagaaatat
       61 tttagcatcc acggtgtact attatagttt caatttacta tagttcaatc attctatcaa
      121 gaatattgaa tatataaagg cacgtaatat taaaacttct tctgatatcg aattagagtc
      181 cgatgttgat ggcgataaat cagacaatct accagtcgac attaagatac tgggacaaca
      241 cattgaagtg tattccgtac ctaagaaaca atcataaaac tcaaagcctt gttaccgagg
      301 cttttttatt atgtgatttt cagtagccag acttattcta tctaatacaa gtaaagctaa
      361 gtatttaaga tttagcagat gatgaagaaa agaaaggatg gcctaaatat tttaacagat
      421 ttacgaggct aatcatggta taatataggg tgatgaaaaa ttccaacgag gctgagatga
      481 aattacttta tactgatatt cgaacttctt tgacagaaat tctaacaaga gaggcagaag
      541 agctagttgc agctggcaag cgggtcttct acattgcccc caactctctt tcttttgaaa
      601 aggaacgcgc cgtgctggaa tgcttgtccc agcaggcttc tttttcgatt accgtcacgc
      661 gctttgctca aatggctcgt tatctggtct tgaatgattt accagttaaa actactcttg
      721 atgatatcgg tcttgggttg gccttttaca aatgccttgc cgaactcgat cccaaggact
      781 tgcgtgttta tggcgctatt aagcaggatc ctcaattgat ccagcagtta attgagcttt
      841 accatgagat gaccaaatct cagatgagtt ttttggactt ggagaattta acagatgagg
      901 ataagagggc ggatttactc ttgatttttg agaaagtaac agcctatctt aatcaaggtc
      961 agttagccca gggaagtcag ttgtcccatt tgattgaggc tattgagaat gacaaggtaa
     1021 gtagtgattt taatcaaatc accttggtca ttgacggctt tactcgtttt tctgctgagg
     1081 aagagcgggt tgtggactta cttcacggca aaggtgttga gattgttatc ggggcttatg
     1141 ctagtaagaa agcctatacc agtcctttta gcgagggcaa tctctaccaa gccagcgtaa
     1201 aatttctcca tcatctggct tctaaatacc aaacgcctgc tcaggactgt tctcaaactc
     1261 atgagaagat ggatagtttt gacaaggcct ctcgtttgtt ggagtcttct tatgactttt
     1321 cagaactcgc tttggatgtc gatgagaaag accgtgaaaa tttacaaatc tggtcttgtt
     1381 tgacgcaaaa ggaggagttg gagctagtag cccgtagcat tcgtcagaaa ttacatgaga
     1441 actcagacct gagctacaag cattttcgta ttctcttggg ggatgtagct tcttaccagt
     1501 tatctctcaa aaccattttt gaccagtatc agattccttt ttatcttggt agaagcgaag
     1561 ccatggctca tcatcccttg actcagtttg tcgagtctat tttagcttta aaacgttacc
     1621 gttttcgtca ggaggatttg attaatcttc ttagaactga tttgtatact gacctcagtc
     1681 agtctgatat tgatgctttt gagcaatata tccgctatct tggtatcaat ggcttgccag
     1741 cctttcagca aactttcacc aaatcccacc atggaaaatt taatcgtgag cgtttgaatg
     1801 tcctccgcct gagaatttta gcacctcttg aaaccctctt tgccagccga aaacaaaagg
     1861 ctgaaaacct cctacaaaaa tggagtgtct ttctaaaaga aggagctgtg accaagcagt
     1921 tacaagattt gacaaccact ttggaagctg tagaacagga aagacaagcc gaagtttgga
     1981 aggctttctg ccatgtttta gaacaatttg cgactgtttt tgctggttca caggttagtc
     2041 tggaagactt cctagccttg ctccattctg gaatgagttt gtcccaatac cgtaccattc
     2101 cagcaacagt ggacactgtt ctggtgcaga gttacgattt gattgcacca ttgactgctg
     2161 actttgtcta tgctattgga ctaactcagg acaatttacc aaaaatttct caaaacacca
     2221 gtcttctgac agatgaagaa aggcaaaacc taaaccaaac gaccgaagaa ggcgttcaat
     2281 tactgattgc cagcagtgaa aatctcaaga aaaatcgcta cactatgctt tccttggtca
     2341 attctgctcg taagcagttg ttcttgtcgg ctccaagcct ttttaacgaa agtgaaagta
     2401 aggaatctgc ctatcttcaa gagttgatcc attttggatt taggcggaga gagaagagga
     2461 tgaatcacaa aggactgtct aaggaggata tggggtccta tcacagtctt ttgtctagtc
     2521 tggttgccta tcaccagcag ggtgagatga gcgatactga gcaagatttg acttttgtca
     2581 aggttctgtc gcgtgtcata ggtaaaaaac tagatcagca aggtctggaa aatccagcca
     2641 tcccaatcag tccaagcagc aagaccttag ccaaggacac cttgcaagct ctctatcctg
     2701 ccaaacagga gttttacctg tctacgtcgg gtttgacaga gttttatcgc aatgaataca
     2761 gttatttcct acgctacgtt ttaggcttgc aggaggaatt acgtttgcgt cctgatgccc
     2821 gtagtcacgg gaatttcttg catcgtatct ttgaacgcgc cttgcagttg cctaatgaag
     2881 attcctttga ccaacgtcta gaacaagcta ttcaagaaac cagtcaagaa cgcgaatttg
     2941 aagctattta tcaagaaagt ttggaagccc agtttaccaa ggaagttttg cttgatgttg
     3001 cacggacaac tggacatatt ctccgacaca atccagccat cgaaacaatc aaagaagaag
     3061 caaattttgg tggaaaagac caagccttta ttcaattaga caatggacgc agtgtctttg
     3121 tacgaggcaa ggtggaccgg attgaccgtt tgaaagctaa tggagcgata ggagtagtag
     3181 actacaaatc cagtctgact cagttccagt ttcctcattt ctttaatggg ctcaattctc
     3241 agttaccaac ctatcttgct gccctaaaaa gagaagggga gcagaacttt ttcggcgcca
     3301 tgtacttgga aatggctgaa cctgtccaat ctctgatggc ggtaaaaagt ctggcaggag
     3361 cagtggtaga agccagcaaa tctatgaaat accaagggct cttcttggaa aaagaaagca
     3421 gttatttagg cgaattttat aacaaaaaca aggcgaatca actgacagat gaggaatttc
     3481 agctcctact ggactacaat gcctatcttt acaagaaagc tgctgagaag attttagcag
     3541 gccggttcgc catcaatcct tatactgaaa atggcagaag cattgcccca tacgtccagc
     3601 aacatcaggc tattacaggc tttgaagcca attaccatct gggccaagcc cgtttcctag
     3661 aaaagttgga cctagctgat ggcaagcgtc tggtcggaga aaaactcaag caagcttggc
     3721 ttgaaaaaat aagagaggag ttgaatcgat gaagcttatt ccctttttaa gtgaggagga
     3781 gattcaaaaa ctgcaagaag cagaagcaaa ttcgagcaag gaacaaaaga aaactgccga
     3841 gcaaatcgaa gctatctaca cttctggtca gaatatcctg gtctcagcat cggctggttc
     3901 tggaaagacc tttgtcatgg cagagcgcat tctggaccaa ttggcgcgtg gtgtcgaaat
     3961 ttctcaactc tttatctcaa cctttaccgt caaggctgca actgaactta aagaacgttt
     4021 agagaaaaaa atcagcaaga aaatccaaga aacagatgat gtcgacctca aacaacactt
     4081 gggacgccag ttggcagacc taccaaacgc tgccatcgga accatggact ctttcacaca
     4141 aaaattcctt ggcaaacatg gttatctgct tgatattgca cctaatttcc gtattttaca
     4201 aaaccaaagc gagcaacttc ttctcgaaaa cgaagtcttt catgaggtct ttgaagcgca
     4261 ttaccaaggt aaacagaaag agacctttag tcatttgctg aaaaactttg ctgggcgtgg
     4321 caaggacgaa cggggtctgc gccagcaggt ctataaaatc tatgacttcc tccaatccac
     4381 cagtaatcct caaaagtggc tgagtgaatc tttcctcaaa ggatttgaga aagctgattt
     4441 taccagtgaa aaagaaaaac tgactgagca aatcaaacaa gccctttggg atttggaaag
     4501 ctttttccgt taccatctgg ataacgatgc caaggagttt gcaaaggctg cctatttaga
     4561 aaatgttcag ttaattctgg atgaaatttg ctccctaaat caggagtccg atagtcaggc
     4621 ttatcaggca gtgcttgcgc gtgttgtcgc catctctaag gagaaaaacg gtcgagctct
     4681 gactaatgcc agtcgtaagg ctgatttgaa gcccctggct gatgcctaca acgaagagag
     4741 aaagacccag tttgctaaac taggacaatt atcagaccag ataaccattc tcgactatca
     4801 agaacgttat catcaagaca catggaaact agctaaaacc ttccaaacct ttatgagtca
     4861 ttttgtggag gcttatcgtc agcgcaaacg ccaggaaaat gcctttgaat tcgctgatat
     4921 cagccattac accattgaga ttttagagaa tttcccacaa gttcgtgagt cttatcagga
     4981 gcgcttccat gaagtcatgg tcgatgagta tcaggatacc aaccatattc aagaacggat
     5041 gctggaattg ttgtctaatg gccacaatcg ctttatggtg ggagatatca agcaatccat
     5101 ctatcgtttc cgtcaggcag acccgcagat tttcaatgag aaattccaac gctatgcgca
     5161 aaatccccaa gaaggcaagc tcattctcct caaggaaaat ttccgtagta gttcagaagt
     5221 gctgtcagca accaatgatg tctttgaacg tctcatggac caagaggtcg gtgaaatcaa
     5281 ctatgataac aagcaccagc ttgtttttgc caataccaaa ctgactccca atccagacaa
     5341 caaggcagaa tttctcctct acgacaagga cgatacaggt gaggaagaag agagtcaaac
     5401 agaaacgaaa ctaacaggcg aaatgcgctt agttatcaag gagattctga aacttcatca
     5461 agaaaaaggt gttgccttta aggaaattgc ccttctgacc tccagccgca gtcgtaatga
     5521 ccagattctc ctcgccctgt ctgagtacgg gattcctgtc aaaactgacg gagagcaaaa
     5581 caattatctc caatccctag aagtgcaagt catgctagac actcttcgtg tcattcacaa
     5641 tcccctgcaa gactacgcct tggttgccct tatgaagtct ccaatgtttg gttttgatga
     5701 ggatgagcta gcacgtttgt cccttcagaa agcagaggat aaagtccacg aaaatctcta
     5761 tgagaaactg gtcaatgcac aaaaaatggc aagtagtcaa aaaggcttga ttcacacagc
     5821 tctagttgaa aaactaaagc aattcatgga tatcctagct tcttggcgct tgtatgccaa
     5881 aacccactct ctctatgact tgatttggaa gatttacaac gaccgttttt attatgacta
     5941 tgttggggct ttgccgaatg gtcctgctag gcaggccaat ctctatgccc tagcactgcg
     6001 tgctgatcaa tttgaaaaga gcaatttcaa aggtttgtcg cgttttattc gtatgattga
     6061 ccaagtctta gaagcccagc acgatttggc aagcgtggcc gtcgcaccgc caaaagatgc
     6121 agtagagctc atgaccatcc acaagagtaa agggctggag tttccttacg tctttatcct
     6181 caatatggat caagatttca acaagcaaga ctctatgtca gaagtcattc tcagtcgtca
     6241 gaatggtctt ggtgtcaaat atattgccaa gatggagaca ggggcagtag aagaccacta
     6301 tcctaaaacc atcaaactct ccattcctag tctgacctat aggcagaacg aagaggaatt
     6361 acagctagca agctattctg agcagatgcg tttgctgtat gttgctatga cgcgggctga
     6421 gaaaaagctc tatcttgtcg gcaagggttc tcgtgaaaag ctggaatcca aggaataccc
     6481 agcagccaaa aatgggaaac taaatagcaa tactagactg caagcacgga atttccaaga
     6541 ttggctttgg gctatcagta aagtgtttac taaggacaag ctcaacttta gttatcgttt
     6601 tattgacgaa gatcagttga ccagagaagc tatcggagag ttggaaacca agagtcctct
     6661 ccaagatagc tcccaagcag acaatcgtca gtcagatacc atcaaagaag ctctggaaat
     6721 gctgaaggag gtggaagttt ataatactct tcaccgcgca gctattgaac ttcctagtgt
     6781 tcaaacccca agtcaaatca agaaattcta cgaaccagtt atggatatgg aaggtgtcga
     6841 gattgctggt caaggtcagt cagtaggcaa gaaaatcagc ttcgatttgc cagatttttc
     6901 aaccaaagaa aaggtaactg gagctgagat tggtagtgct actcacgaac tcatgcagat
     6961 aattgacctc agccagcaac taacccttgc tagcttaaca gaaacactca aacaagttca
     7021 aactagccaa gctgtcagag acaagatcaa tcttgataaa attcttgctt tctttgacac
     7081 agcactcggt caggaaattc ttgctaatac cgaccatctc tatcgcgagc aacctttctc
     7141 catgctcaaa cgagaccaaa agagtcagga agactttgtt gtccgtggta tccttgatgg
     7201 ctatctgctt tacgaaaaca aaattgttct gttcgactac aagacagacc gctatgatga
     7261 accaagtcaa ctcgtagacc gctatcgtgg tcagttagct ctatacgaag aggctttatc
     7321 acgagcctat tcgattgaaa atattgaaaa atacttgatt ttactcggta aagacgaggt
     7381 tcaagttgta aaagtataac ttatactctt cgaaaatctc ttcaaaccac gtcagcttcg
     7441 c
//
LOCUS       DAVBHS010000100         7382 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVBHS010000100 DAVBHS010000000
VERSION     DAVBHS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.100"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            57..842
                     /locus_tag="VQ651_001532"
     CDS             57..842
                     /locus_tag="VQ651_001532"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET2754506.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            936..1370
                     /locus_tag="VQ651_001533"
     CDS             936..1370
                     /locus_tag="VQ651_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2754507.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            1370..2344
                     /locus_tag="VQ651_001534"
     CDS             1370..2344
                     /locus_tag="VQ651_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HET2754508.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANNRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            2404..2628
                     /locus_tag="VQ651_001535"
     CDS             2404..2628
                     /locus_tag="VQ651_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET2754509.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            2747..3721
                     /gene="fabK"
                     /locus_tag="VQ651_001536"
     CDS             2747..3721
                     /gene="fabK"
                     /locus_tag="VQ651_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HET2754510.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            3714..4634
                     /gene="fabD"
                     /locus_tag="VQ651_001537"
     CDS             3714..4634
                     /gene="fabD"
                     /locus_tag="VQ651_001537"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET2754511.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            4668..5399
                     /gene="fabG"
                     /locus_tag="VQ651_001538"
     CDS             4668..5399
                     /gene="fabG"
                     /locus_tag="VQ651_001538"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET2754512.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            5421..6656
                     /gene="fabF"
                     /locus_tag="VQ651_001539"
     CDS             5421..6656
                     /gene="fabF"
                     /locus_tag="VQ651_001539"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET2754513.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            6659..7144
                     /locus_tag="VQ651_001540"
     CDS             6659..7144
                     /locus_tag="VQ651_001540"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET2754514.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            7141..>7382
                     /gene="fabZ"
                     /locus_tag="VQ651_001541"
     CDS             7141..>7382
                     /gene="fabZ"
                     /locus_tag="VQ651_001541"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET2754515.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKL"
BASE COUNT         2188 a         1318 c         1806 g         2070 t
ORIGIN      
        1 ggtttaaaga gattttcgaa gagtataaac tgcttataaa ataaaaagga gccctgatgg
       61 aacacattat ttatcagctt gaagaggact tggcaatcct taccttgaac cgtcctgagg
      121 tcgcaaatgg ttttcatatt cccatgtgtg aggagatttt agaagctctg actttggcag
      181 aagaaaatcc agctgtgcat tttatcttaa tcaatgccaa tggaaaggtc ttctcagttg
      241 ggggagattt ggtagagatg aagcgggcag tggatgagga tgatattcca tcattgacaa
      301 aaatcgcaga gttggtcaat acgatttctt ataaaatcaa gcaaatagcc aaacctgttt
      361 taatggaagt tgacggtgct gttgcaggtg ccgcagcgaa tatggctgtt gcggcagatt
      421 tctgtttggc gacggataag gctaagttta tccaagcctt tgttggtgtt ggtttggctc
      481 cagatgcagg gggaattcat ctcttgagcc gcagtatcgg tgtgacgcgt gctgctcaat
      541 tagctatgac aggagaggct ttaacggcag aaaaagccct agaatggggg ttggtttacc
      601 gcgtttctga agctgaaaaa cttgaaaaga cgagagaaca gcttcttaaa aaattaagac
      661 gtgcttcaag taattcctat gctgccatta agaagttggt ttgggagagc caatttaaag
      721 actggcaggg ttacgctact ttagaactga acctacagaa atccttagct caaacagagg
      781 atttcaaaga aggagtgcga gctcattcgg agagaagaag acctaaattt acaggaaaat
      841 aaaaaatact tgcatcattc tttgaatttt gatataattc tttctgtcaa atgttttgat
      901 tgtaaaagtt ttttgaagaa ggagggatat gaaaaatgga ctaccaacga atcaatgaat
      961 atttaacatc tatatttaac aatgtccttg taattgagga agtgaacttg agaggtagtc
     1021 gttttaagga tatctccatc aaagaaatgc atacgattga tgtcatcggt aaggctccag
     1081 atgtgactcc aagtcaagtg tcaaaagagt tgatggtaac tcttggaact gttacgacaa
     1141 gtttgaacaa tttagagcgt aagggctaca ttgagcgagt tcggtcagaa caggatcgtc
     1201 gtgtggtgca tctgcatttg acaaagaagg gtcgcttgat tcatagactg cataaacgct
     1261 tccacaaggc catggtagaa aaaattattg atggcatgag cgaggaagaa attgctgtca
     1321 tgggtaaagg tttgactaat ctttaccaat ttttggagga tttgaaataa tggcttttgc
     1381 aaaaataagt caggttgctc attatgtgcc agagcaagtg gttacaaatc acgacttggc
     1441 tcagattatg gataccaatg atgagtggat ttcaagtcga acgggaatac gacaaaggca
     1501 tatttcaaga acagaatcta ccagtgattt ggctacagag gttgctaaga aactgatggc
     1561 aaaagctgga ataacaggag aagaactgga ttttatcatc ctagctacca ttactccaga
     1621 ttcgatgatg ccctctacag ctgctcgtgt tcaagctaat attggtgcta ataaagcctt
     1681 tgcttttgac ttaaccgcgg cttgcagtgg atttgtattt gctctttcaa ctgctgaaaa
     1741 gtttatcgct tctggtcgct ttcaaaaagg cttggtgatt ggtagtgaaa ccctctctaa
     1801 ggcagtcgat tggtcggatc gatcaacagc tgtgttgttt ggagatggtg ctggtggtgt
     1861 cttgttagaa gctagcgagc aagagcattt cttagctgag agtctcaata gcgatggaag
     1921 tcgcagcgag tgtttaactt atgggcattc aggtttgcat tctccatttt cagatcaaga
     1981 aagtgcagat tcatttttga agatggatgg acgcacagtc tttgattttg ccattcgaga
     2041 tgtagccaag tctatcaagc agactattga tgaatctcct atagaggtga cagacttgga
     2101 ttatctgcta cttcatcaag ccaataaccg tattttggat aagatggcta gaaaaattgg
     2161 tgttgaccga gccaaacttc cagctaatat gatggaatat ggcaatacca gtgcagccag
     2221 tatcccgatt ttactttcag agtgtgtaga acaaggtctc atccctttag atggtagcca
     2281 aactgttctt ctatcaggct tcggtggagg cttgacctgg ggcacgctca ttcttacaat
     2341 ttaggtaatc atgtggtgaa cacattgtta taaaaaaact atttatttta aaggagtcct
     2401 atcatggcag tatttgaaaa agtacaagaa attatcgttg aagaacttgg aaaagacgca
     2461 tcagaagtaa cacttgaatc aacttttgat gatttggacg cagattcatt ggacttgttc
     2521 caagtaatct cagaaatcga agatgctttt gatatccaaa tcgaagcaga aaatgacttg
     2581 aaaacagttg gtgacttggt tgcttacgtt gaagagcaag caaaataagc agaatattag
     2641 tagaaggagt agggagaaac ccactccctc ttgtttaggt aatagtttga ctgtcaaatt
     2701 atggtgaaat cgaactatta cgtaagcaag gaaggttgga ggcactatga aaacgcgtat
     2761 tacagaatta ttgaagattg actatcctat tttccaagga gggatggcct gggttgctga
     2821 tggtgatttg gcaggggctg tttccaaggc tggaggatta ggaattatcg gtgggggaaa
     2881 tgccccgaaa gaagttgtca aggccaatat tgataaaatc aaatcattga ctgataaacc
     2941 ctttggggtc aacatcatgc tcttatctcc ctttgtggaa gatatcgtgg atctcgttat
     3001 tgaagaaggt gttaaagttg tcacaacagg agcaggaaat ccaagcaagt atatggaacg
     3061 tttccatgaa gctgggataa tcgttattcc tgttgttcct agtgtcgctt tagctaaacg
     3121 catggaaaaa atcggtgcag acgctgttat tgcagaagga atggaagctg gggggcatat
     3181 cggtaaatta acaaccatga ccttggtgcg acaggtagcc acagctatat ctattcctgt
     3241 tattgctgca ggaggaattg cggatggtga aggtgctgcg gctggcttta tgctaggtgc
     3301 agaggctgta caggtgggga cacggtttgt agttgcaaaa gagtcgaatg cccatccaaa
     3361 ctacaaagag aaaattttaa aagcaaggga tattgatact acgatttcag ctcagcactt
     3421 tggtcatgct gttcgtgcta ttaaaaatca gttgactaga gattttgaac tggctgaaaa
     3481 agatgccttt aagcaggaag atcctgattt agaaatcttt gaacaaatgg gagcaggtgc
     3541 cctagccaaa gcagttgttc acggtgatgt ggagggtggc tctgtcatgg caggtcaaat
     3601 cgcagggctt gtttctaaag aagaaacagc tgaagaaatc ctaaaagatt tgtattacgg
     3661 agccgctaag aaaattcaag aagaagcctc tcgctgggca ggagttgtaa gaaatgacta
     3721 aaacagcctt tttatttgct ggtcaaggtg cccagtatct agggatggga cgggatttct
     3781 atgatcagta tccgattgtt aaagaaacga ttgatcgagc gagtcaggtg ctcggttatg
     3841 atttacgtta tctcatcgat acggaagaag acaaactcaa tcagacccgc tatacgcaac
     3901 cagccattct agcgacttcg gttgctatct accgtttatt gcaagaaaag ggctatcagc
     3961 ctgatatggt tgctggtttg tctcttggag aatactctgc cttggtggca agcggcgcct
     4021 tggattttga agatgcggtt gccttggtag ctaagcgtgg agcctatatg gaagaagcgg
     4081 ctcctgctga ctctggcaag atggtagcag ttctcaatac gccagtagag gtcattgaag
     4141 aagcctgtca aaaagcttct gaacttggag tggttactcc agccaactat aacacacctg
     4201 cacaaatcgt cattgctgga gaagtggttg cagttgatcg agcggttgaa cttttgcaag
     4261 aagcaggtgc caaacgcttg attcctctta aggtgtcagg tccctttcac accgctctcc
     4321 ttgagcctgc tagccagaaa ctagctgaaa ctctagctca ggtaagtttt tcagatttta
     4381 cttgtcccct agtcggcaat acagaagctg ctgtgatgca aaaagaggac attgctcagc
     4441 tcttgacgcg tcaggtcaag gaacccgttc gtttctatga aagtattggg gtcatgcaag
     4501 aagcaggcat aagcaacttt atcgagattg gaccggggaa agtcttgtca ggttttgtta
     4561 aaaaaattga tcaaactgct cacttagctc atgtggaaga tcaagcgagt ttagtagcac
     4621 ttttagaaaa atagactaaa ataagtagaa gttttgaaag gaaaaaaatg aaactagaac
     4681 ataaaaatat ctttattaca ggttcgagtc gtggaattgg tcttgccatc gcccacaagt
     4741 ttgctcaagc aggagccaac attgtcttaa acagtcgtgg ggcaatctca gaagaattgc
     4801 tcgctgagtt ttcaaactat ggtatcaagg tggttcccat ttcaggagat gtatcagatt
     4861 ttgcagacgc taagcgtatg attgatcaag ctattgcaga actgggttca gtagatgttt
     4921 tggtcaacaa tgcagggatt acccaagata ctcttatgct caagatgaca gaagcagatt
     4981 ttgaaaaagt gctcaaggtc aatctgactg gtgcctttaa tatgacacaa tcagtcttga
     5041 aaccgatgat gaaagccaga gaaggtgcta tcattaatat gtctagtgtt gttggtttga
     5101 tggggaatat tggtcaagct aactatgctg cttctaaggc tggcttgatt ggctttacca
     5161 agtctgtggc acgcgaggtc gctagtcgga atatacgagt caatgtgatt gctccaggaa
     5221 tgattgagtc tgatatgaca gctatcttat cagataagat taaggaagct acactagctc
     5281 agattccgat gaaagaattt gggcaggcag agcaggttgc agatttgaca gtatttttag
     5341 caggccaaga ttatctaact ggtcaagtga ttgccattga tggtggctta agtatgtagc
     5401 gaaagctaga ggtgaaaaga atgaaactaa atcgagtagt ggtaacaggt tatggagtaa
     5461 catctccaat cggaaataca ccagaagaat tttggaatag tttagcaact gggaaaatcg
     5521 gcattggtgg cattacaaaa tttgatcata gtgactttga tgtgcataat gcggcagaaa
     5581 tccaagattt tccgttcgat aaatactttg taaaaaaaga taccaaccgt tttgataact
     5641 attctttata tgccttgtat gcagcccaag aggctgtaaa tcatgccaat cttgatgtag
     5701 aggctcttaa tagggatcgt tttggtgtta tcgttgcatc tggtattggt ggaatcaagg
     5761 aaattgaaga tcaggtactt cgccttcatg aaaaaggacc caaacgtgtc aaaccaatga
     5821 ctcttccaaa agctttacca aatatggctt ctgggaatgt agccatgcgt tttggtgcaa
     5881 acggtgtttg taaatctatc aatactgcct gctcttcatc aaatgatgcg attggggatg
     5941 ccttccgctc cattaagttt ggtttccaag atgtgatgtt ggtgggagga acagaagctt
     6001 ctatcacacc ttttgccatc gctggtttcc aagccttaac agctctctct actacagagg
     6061 atccaactcg tgcttcgatc ccatttgata aggatcgcaa tgggtttgtt atgggtgaag
     6121 gttcagggat gttggttcta gaaagtcttg aacacgctga aaaacgtgga gctactatcc
     6181 tggctgaagt ggttggttac ggaaatactt gtgatgccta ccacatgact tctccacatc
     6241 cagaaggtca gggagctatc aaggccatca aactagcctt ggaagaagct gagatttctc
     6301 cagagcaagt agcctatgtc aatgctcacg gaacgtcaac tcctgccaat gaaaaaggag
     6361 aaagtggtgc tatcgtagct gttcttggta aggaagtacc tgtatcatca accaagtctt
     6421 ttacaggaca tttgctgggg gctgcgggtg cagtagaagc tatcgtcacc atcgaagcta
     6481 tgcgtcataa ctttgtacca atgacagctg ggacaagtga agtatcagat tatatcgaag
     6541 ctaatgtcgt ttatggacaa ggcttggaga aagaaattcc atacgctatt tcaaatactt
     6601 ttggttttgg aggccacaat gcagttcttg ctttcaaacg ttgggagaat agataagtat
     6661 gaatttaaac gatattaaag acttgatgac tcaatttgac cagtcaagtt tgagagaatt
     6721 ttcttataaa aatgggacgg atgagttgca gtttagcaag aatgaagcga gacctgtgcc
     6781 tgaagttgca actcaagtcg ctccagcacc cgttctagca acaccgagtc cagtagctcc
     6841 tacatctgct ccagcagaga ctgtagcaga agaagttcca gctccagctg aagcaagtgt
     6901 ggctagtgag ggaaatcttg tagagagtcc acttgttgga gtggtttact tggctgctgg
     6961 accagataaa cctgccttcg ttacagttgg tgatagtgtc aaaaaaggtc aaacattggt
     7021 aattatcgaa gccatgaaag tcatgaatga aatcccagct cctaaggatg gtgtggtaac
     7081 ggaaattctc gtctctaacg aagaaatggt tgagtttggt aaaggattgg tacgtatcaa
     7141 atgatcgata ttcaaggaat caaagaagcc cttccccacc gttatcctat gcttctagtg
     7201 gaccgtgtct tggaggtgag cgaggatacc attgttgcta tcaaaaatgt gaccatcaac
     7261 gagcctttct ttaacggcca ctttcctcaa tacccagtta tgccaggtgt tctgattatg
     7321 gaagccttgg cgcaaactgc tggtgtgttg gagttatcaa aacctgaaaa taaaggaaaa
     7381 ct
//
LOCUS       DAVBHS010000101         7264 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVBHS010000101 DAVBHS010000000
VERSION     DAVBHS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.101"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..829)
                     /locus_tag="VQ651_001542"
     CDS             complement(<1..829)
                     /locus_tag="VQ651_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2754516.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKSTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIIAGISNGAV
                     "
     gene            complement(843..1709)
                     /locus_tag="VQ651_001543"
     CDS             complement(843..1709)
                     /locus_tag="VQ651_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2754517.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(1723..2982)
                     /locus_tag="VQ651_001544"
     CDS             complement(1723..2982)
                     /locus_tag="VQ651_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2754518.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADDTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRSE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(3074..5236)
                     /locus_tag="VQ651_001545"
     CDS             complement(3074..5236)
                     /locus_tag="VQ651_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET2754519.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATITSYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIILSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYFYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            5344..6204
                     /locus_tag="VQ651_001546"
     CDS             5344..6204
                     /locus_tag="VQ651_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET2754520.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFYYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            6201..7136
                     /locus_tag="VQ651_001547"
     CDS             6201..7136
                     /locus_tag="VQ651_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HET2754521.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
BASE COUNT         2312 a         1516 c         1310 g         2126 t
ORIGIN      
        1 tcactgcccc gttgcttatt cctgcgatga tatggcgttg gaagaagaga tagacaatgg
       61 tgatactgat aatgccgacc acgtaagagg caaagcttgg tccgtagtcg ttgaaatatt
      121 ggcctgcgta gttgtattgg aacaaaggca gagtccacat tttggaatcc cggttcaaga
      181 caaggagtgg caacatgaag tcattccaga accaaagggc attgatgatc atggttgtcg
      241 catgcatcgg tttcatcatt gggaagatga tgcggaaata ggttgtaaat tgattagccc
      301 catcgatctc tgctgcttca tccagacttt ctggaatcga gattttgata tagccaacat
      361 agagaaagag ggtctgtgga atcgcatagg tcaagtagag caagatcaaa ccaaaggtat
      421 tagccaaacc gagtttactc atcataaccg taatcggaat catgatgact tggaaaggta
      481 cgaagattcc gaggattaag agggtataca tgatggtaaa ggcttttctt ttactcatat
      541 tgcgagcgat ggagtaggct gccataggga taaagatcat tactgcaagt aaagacaaga
      601 cagtgatgac gacagagttc caataatagc ctccaatccc atcagctaag agacggctaa
      661 agttgtccca tgtgaagttg gttggaaagc caaagaaatt atctacaata tccttagtgg
      721 atttgaagga actaaagagg gtagcaagga gcggcactaa aatcagaacc gatcctagaa
      781 tcaatagaat gtatttgcca atcagggctt ttctttcatc ttgtttcatc atgcttctcc
      841 tcttaaattt caaatttctt agatactctc aattggatga tcgaaatcac tacaattaag
      901 aagaacaaga ttacggcaat ggcattggca taaccgaatt ggttgttttt aaaggcatag
      961 ttataaacca agagcccaag tgaggttgtg gcattgtttg gaccaccacc ggtcatggca
     1021 aagacttggt caaaggcagt cagcccacct tttagggcta ggataaagac tatagagaca
     1081 cttggtagca agtaaggcaa ttcaatgttc cagaaaactt gcttgctagt cgcaccatca
     1141 atccttgctg cctctgtaat ctcagttgga atagattgca aaccagctag gaagatgatg
     1201 atgggcatag ccaccccttg ccaaagaagg acaaagacag ccgcaaagat tgctccccac
     1261 ttagtcccta aaagactggt ttggagaaat tcaatatgaa gggcattccc aatcgctgga
     1321 agaccgtagt tgaagacttg cttgaagatc aaagccactg tcaaaccaga taaaacagct
     1381 gggaagaaga accaagcacg gaagaaggtt tggcctttga ttttagagtt caagacacgc
     1441 gcaatgaaga tcccgagtgc aatctcacca accaccatgg caatcgtaat gattgcggta
     1501 aagccaatcg cattcatgaa ttttggatcc atgaagagga gcttaaagtt gtttaagccg
     1561 acaaatttgt agttgtaagt caatcctgtc cagttggtaa aactgtaaaa ggctccttga
     1621 aacatcggca catagaagaa aattgcttgt aacaagaggg ggatgaccac aaaagcccat
     1681 gcccaatatt tttgtaatac ttttttcata gtctctctac tcctaatcca catccgcttt
     1741 catcgggtta aagaaggcat tcaaatcatt gaccatgcct tgtttatcac cggtcaagac
     1801 atagttcatg gtcaaggtat ggaagtctgc ttcactggtc cagtattgtt gcaaccagac
     1861 caagtgacga tccgtaaagg catattcggt cataccagca agcggtgaat cttctcctgc
     1921 ttgtttgacc ccttcgatcg ctgttggaga tccgtccaca tcgtagtatt tttgcatgac
     1981 ttctgaacgg gtcatatatt ccacaaaggc attggcttct tttggatgtt tggtggtggc
     2041 tgagatagac catgccaagt ctcccgcacc aacggttaag ctttgtcctt tttcttttcc
     2101 tggaatcatg aaggttccaa tcttaaagtt cggtttttgt tcattaatcg ctgtgatcgc
     2161 ccaagaccca tttggtgtca tgaggacatc cccacgtgcg aaggctccga taacatcggt
     2221 atagccagca ccttcccagt tcttttgctt agagccatta atgcgaagga tgtccatgac
     2281 cttgatatca tctttcataa tcggatccga caatttaata gcatttggtt gagaataacg
     2341 aaggtattga tttgcttctt ttcctccacc tgttgctgtc gcaaaggcta attgattgta
     2401 accattgagt gtccaagcat ctgcacctgc aattccaaat ggtgtttgtc ctttagcaac
     2461 gatatctttg actaactgtt caaattcatc ccaggtttca ggaaccttca agcccagttc
     2521 ttcgaattta tctttgttgt agtaaattcc ataagcatta gctgtaaaag gaacgttgta
     2581 aactttttcg tttacagcat atttttcagc gtagccattt ttcacgcgtt tcaggtagtc
     2641 tttgttactc aaatcttcaa agacacctgc ttttgcccat tcttgcagtt cgatggactg
     2701 tgggtaaata ttgaccacat caggcacatc tcctgcgaga acgcgtgtct tcaatacttc
     2761 accagcattt ggtacattga cgactttgac ctttatctta gggttttcct tctcaaaatc
     2821 acgagtgatt tcttccaagg ttttggtcat ttcttttttc tggttgaaat actcgatggt
     2881 cactgtgtca tccgcagatt taccatagtt ggagcaagca ccgagcccaa acaaagctaa
     2941 acctgtagtt gcaagaagtc cgattttttt ataccattcc attagaaagc ctcctttata
     3001 aatttataca cccttatcaa actgcacttc aaaagttaga tagaataaat ctaacttttg
     3061 gggtcagtac atatcatagt tttctaaaaa tatactgtct actcaaaaaa tctccttggg
     3121 ataagataac agttaagccc gcatacatta gttctgcacc tgagtaaact tcgccatttt
     3181 cctgtaattt atatagtccc tcttcatcca aatcttttaa ttttaaagtt gtttccatgg
     3241 tctctacaac agataaaacg cgaacgtagg ttacaatcgt ttgatttccg taattaaatt
     3301 gtacagctgc ttcattggat gcagtatcag gattaattag tctatactgc tgtcctaact
     3361 gaactactgg tcgtaattct ttatacaagt tcacctgatt agcaatcgta gctttctctt
     3421 catctgataa atttgtcaaa tcaagctcat agcccaaatt tcccatcatt gctacatggc
     3481 cacgtgtttc taatggtgtc attcgtccca tctgatgatt cggtactgct gacacatgag
     3541 cccccataga aatggttgga tagaaatagg atgaaccgta ttgaattggt aaacgtgcaa
     3601 tggcatcagt attatcacta gcccagactt gtgggaaata gcgtatcata ccaagatcat
     3661 ttcgtccacc accaccagag caggactcaa agagaatatg gctgtgcttc tctgtcagat
     3721 aagaaacgag ttcataaagc cccagcatgt actgatgaga ttgcatctgt gtctctagat
     3781 aagttaatcc attccctagc ttagtgatat tgcggttcat atcccattta atgtaatcaa
     3841 tctcatgata agataggagt tgatctaaga cacttttcaa gtattctact acctgaggat
     3901 tggcaagatt aagtactaat tgattccgag aataagtatg ctcatagtca ggaacctgaa
     3961 tagcccagtc agggtgttga cgatacaaat cactatctac agaaatcatt tcgggttcta
     4021 accaaagtcc aaactgcaaa cctctttcat ggatagctga aatcagactt tctagacttc
     4081 cacccagttt ttcctcatta acaacccaat cacctaaagc acgattatca tcaaaacgat
     4141 tgccaaacca accatcatct aatacaaaaa gttcaatgcc aactttctta gcttcatctg
     4201 ctaactctaa cagtttttct ctctgaaagt caaagtaagt agcttcccag ttattgatta
     4261 gaattggacg ttctttttta gaaaattcac ttagaataat gtgcttcagt acaaaattct
     4321 gactttcatg actaatacca gttaatccct gatctgaatg agtcactaaa gctaccggtg
     4381 tttcaaagta ttcctcaggg gctaacttcc aagaaaagtt ttctggatta atgccaatag
     4441 ccacccgaac ttcattcaat tgattttttt gaacaaaagc ttcaaagttg ccactataca
     4501 ttagttgaat agcaaacaca ttcccagcat cctctgtgac tccttgttcg catagtagaa
     4561 gagctggtgt ttgagcatga ccagaagcac ctcggtttga actaatcgaa aagattcctt
     4621 gttctacctg ttgacgtcta acagtctttt cacgagcata agcaccctgc agagttgcta
     4681 tttcgtaatc tgcagctgga aaatcagcca taaaagaaaa atctttatgg atgacaactt
     4741 cctgattact attattatct aatttactgt agctagtaat agtcgcatca ttattaaaag
     4801 tagtataata caaagtcaga ctaagttgag ccttagaatc ttctaacatt aagacaagag
     4861 tctctgtatc gtccatgcta tgtggagaag gtaagccctg tggaccattc tgacctttta
     4921 aaatctttgc ttctacaaat cgaaagtctg ttacttcagt tacactatgc tgaacctgta
     4981 tagttggttt cctaaaatct cctaagccat gttgtccaaa aatctgtcgc tgagtatcta
     5041 aactaaaggt tcgattagta gccgttggat ttcctgaaaa ggcatggtct cgttcataaa
     5101 cactattgga acctttatag ttcttaatag tctttcctaa atgtttcaaa agtaagtagc
     5161 catttcgatt ttcaataatc aaacttagat ttttactctc aacataaaat agattattct
     5221 ctatcctaac tcccatttac ttcacctcat cactttattg attatatttt atcacctgaa
     5281 atcgctttcc aaaatagaaa aatgtctcaa gaatatggta aaatgttagg taggaggtag
     5341 cacatgttag ttttttcaga ataccagact ggaacaatcg accttgccct aagcttttat
     5401 ggatatgagg aatgcacacc taattactct tttggtccag ccattcgtga tacatacgtt
     5461 ctacattaca ttactaaagg acaaggaaaa ttttattaca agggtaaaat tgttgattta
     5521 aaagaaggag atttctttct attaaaacca gaggaactaa ccttttatca agcagatagt
     5581 aaagaacctt gggcctacta ctggttagga atcactggag ggaaatcccc tgattatttt
     5641 gctctttctc aaatttctga tcaatcctat ctcatccaat ctgaaacttg tcatacccag
     5701 actactgcaa aactcatctc agacattgtc cgcttcgctc agattacaaa atcaagtgaa
     5761 ttagctcaac tccatatcat gggacaactt catgaactga tgtttcatct gggaactatt
     5821 gctcccaatc agaaaaaaaa gaatatttca tcaacccacc aactctatct tgaatgcaaa
     5881 cgattaattg atagccacta tcctcaatca cttacaattc aagatttagc aaaagaacta
     5941 tccgttcaca gaagctactt atcaagcgta ttcaaagaat ttaatacctt atcacccaaa
     6001 gaatacctac tctacgttcg aatgcaccga gctagacaac ttctcgaaaa tacccaagag
     6061 tccatcaagg taattgcata ctcggtaggt ttttcagatc cactccattt ttcgaaagct
     6121 tataaacaat actttaatca gactccaagt catacaagaa aagaatactc tcaataccaa
     6181 ctagtgagaa aggcaacatt atgaaatcct accaagctgt ctaccaaatc ctatctaaag
     6241 aaaccgacta tatcagcgga gaaaaaatcg cagaaaaact atccctaagc cgaacagcaa
     6301 tttggaaagc catcaagcga ctagaacaag aaggcattga aattgatagt atcaaaaata
     6361 gaggatataa actgatgaat ggtgacctta ttcttccaga gattctagaa gaaaatcttc
     6421 caattaaagt cagctttaaa cccgaaacaa aatcaacaca actagatgca aaagaagcaa
     6481 ttgatttagg ccatgaagca aataccctct atctagcttc ctatcaaaca gcaggccgag
     6541 gccgttttca acgttccttc tactcaccac aaggtggtat ttatatgaca ctccatctta
     6601 aaccaaatct cccctatgac aaattaccat cctacacact acttgtagct ggagctgtct
     6661 acaaagccat taagaaccta actttaatag atgtcgacat aaaatgggtc aatgatatct
     6721 atctaaacaa tcataaaatt ggaggaatcc ttactgaagc aatgacctct gtagaaactg
     6781 gcttagtcac agatatcatt attggagtag gtatcaattt cactattaaa gacttccctc
     6841 aggaattaaa agaaaaagct gccagcttat ttaaagctac agctcctata acaaggaatg
     6901 aattgatcat agaaatctgg cgtgctttct tcgaaacacc agcagaagag ctattatacc
     6961 tatacaaaaa acagtcattc attctaggaa aagaagtcac tttcacacta gagcaaaaag
     7021 actacaaggg acttgctaaa gacatctcag aaaatggaaa acttttagtt caatgtgata
     7081 acggaaaaga aatctggcta aatagtggcg aaatttctct caatagttgg aagtaaaata
     7141 acacaattat aatataaacg atataaaaat aacttcagat tagtaattca attaagtttt
     7201 acggatctga agttttattg tctctaaaaa caaaaaagag agttacaaac tctcattaaa
     7261 acgg
//
LOCUS       DAVBHS010000102         7095 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVBHS010000102 DAVBHS010000000
VERSION     DAVBHS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.102"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            145..6831
                     /locus_tag="VQ651_001548"
     CDS             145..6831
                     /locus_tag="VQ651_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET2754522.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTVEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQTSNQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDVDVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGFKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVARVRTLIGKEPKMPKTVGFVYSDGSREKLPVTWSQ
                     VDVSQAGVVTVKGTANGREVEAHVEVLAIAKELPTVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRIEQYA
                     KEGVFTVNGRLEDTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQIRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLRFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGKERIFT
                     AINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSEASQTN
                     KAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
BASE COUNT         2336 a         1364 c         1581 g         1814 t
ORIGIN      
        1 catatctacg gcaaggcgac actgacgaag tttgaattgg attttcgcag agtgtaaatt
       61 gttgaaacta ggttgtcata ccatgtatac cacttgggct ttggtagtag ctaactgcac
      121 taaatataat ataaggaggg ttttatgggg aaaggccatt ggaatcggaa aagagtttat
      181 agcattcgta agtttgctgt gggagcttgc tcagtaatga ttgggacttg tgcagttcta
      241 ttaggaggaa atatagctgg agaatctgta gtttatgcgg atgaaacact tattactcat
      301 actgttgaga aacctaaaga ggaaaaaatg atagtagaag aaaaggctga taaagctttg
      361 gaaactaaaa atgtagttga aaggacagaa caaagtgaac ctagttcaac tgaggctatt
      421 gcatctgaga agaaagaaga tgaagccgta actccaaaag aggaaaaagt gtctgctaaa
      481 ccggaagaaa aagctccaag gatagaatca caaacttcaa atcaagaaaa accgctcaag
      541 gaagatgcta aagctgtaac aaatgaagaa gtgaatcaaa tgattgaaga caggaaagtg
      601 gattttaatc aaaattggta ttttaaactc aatgcaaatt ctaaggaagc cattaaacct
      661 gatgtagacg tatctacgtg gaaaaaatta gatttaccgt atgactggag tatctttaac
      721 gatttcgatc atgaatctcc tgcacaaaat gaaggtggac agctcaacgg tggggaagct
      781 tggtatcgca agactttcaa actagatgaa aaagacctca agaaaaatgt tcgccttact
      841 tttgatggcg tctacatgga ttctcaagtt tatgtcaatg gtcagttagt ggggcattat
      901 ccaaatggtt ataaccagtt ctcatatgat atcaccaaat accttcacaa agatggtcgt
      961 gagaatgtga ttgctgtcca tgcagtcaac aaacagccaa gtagccgttg gtattcagga
     1021 agtggtatct atcgtgatgt gactttacaa gtgacagata aggtgcatgt tgagaaaaat
     1081 gggacaacta ttttaacacc aaaacttgaa gaacaacaac atggcaaggt tgaaactcat
     1141 gtgaccagca aaatcgtcaa tacggacgac aaagaccatg aacttgtagc cgaatatcaa
     1201 atcgttgaac gaggtggtca tgctgtaaca ggcttagttc gtacagcgag tcgtacctta
     1261 aaagcacatg aatcaacaag cctagatgcg attttagaag ttgaaagacc aaaactctgg
     1321 accgttttaa atgacaaacc tgccttgtac gaattgatta cgcgtgttta ccgtgacggt
     1381 caattggttg atgctaagaa ggatttgttt ggttaccgtt actatcactg gactccaaat
     1441 gaaggtttct ctttgaatgg tgaacgtatt aaattccatg gagtatcctt gcaccacgac
     1501 catggggcgc ttggagcaga agaaaactat aaagcagaat atcgccgtct caaacaaatg
     1561 aaggagatgg gagttaactc catccgtaca acccacaacc ctgctagtga gcaaaccttg
     1621 caaatcgcag cagaactagg tttactcgtt caggaagagg cctttgatac ttggtatggt
     1681 ggcaagaaac cttatgacta tggacgtttc tttgaaaaag atgccactca cccagaagct
     1741 cgaaaaggtg aaaaatggtc tgattttgac ctacgtacca tggtcgaaag aggcaaaaac
     1801 aaccctgcta tcttcatgtg gtcaattggt aatgaaatag gtgaagctaa tggtgatgcc
     1861 cactctttag caactgttaa acgtttggtc aaggttatca aggatgttga taagactcgc
     1921 tatgttacca tgggagcaga taaattccgt ttcggtaatg gtagcggagg gcatgagaaa
     1981 attgctgatg aactcgatgc tgttggattt aactattctg aagataatta caaagccctt
     2041 agagctaagc atccaaaatg gttgatttac ggttcagaaa catcttcagc tacccgtaca
     2101 cgtggaagtt actatcgccc tgaacgtgaa ttgaaacata gcaatggacc tgagcgtaat
     2161 tatgaacagt ctgactatgg aaatgatcgt gtgggttggg ggaaaacagc aaccgcttca
     2221 tggacttttg accgtgacaa cgctggctat gctggacagt ttatctggac aggtacggac
     2281 tatattggtg aacctacacc atggcacaac caaaatcaaa ctcctgttaa gagctcttac
     2341 tttggtatcg tagatacagc cggcattcca aaacatgact tctatctcta ccaaagccaa
     2401 tgggtttctg ttaagaagaa accgatggta caccttcttc ctcactggaa ctgggaaaac
     2461 aaagaattag catccaaagt agctgactca gaaggtaaga ttccagttcg tgcttattcg
     2521 aatgcttcta gtgtagaatt attcttgaat ggaaaatctc ttggttttaa gactttcaat
     2581 aaaaaacaaa ccagcgatgg gcggacttac caagaaggtg caaatgctaa tgaactttat
     2641 cttgaatgga aagttgccta tcaaccgggt accttggaag caattgctcg tgatgaatct
     2701 ggcaaggaaa ttgctcgaga taagattacg actgctggta agccagcggc agttcgtctt
     2761 attaaggaag accatgcgat tgcagcagat ggaaaagact tgacttacat ctactatgaa
     2821 attgttgaca gccaggggaa tgtggttcca actgctaata atctggttcg cttccaattg
     2881 catggccaag gtcaactggt cggtgtagat aacggagaac aagccagccg tgaacgctat
     2941 aaggcgcaag cagatggttc ttggattcgt aaagcattta atggtaaagg tgttgccatt
     3001 gtcaaatcaa ctgaacaagc agggaaattc accctgactg cccactctga tctcttgaaa
     3061 tcgaaccaag tcactgtctt tactggtaaa aaagaaggac aagagaagac tgttttgggg
     3121 acagaagtag caagagttcg tacattaatt ggtaaagaac caaaaatgcc gaaaacagta
     3181 ggttttgtat acagcgatgg tagtcgtgaa aaacttccag taacttggtc tcaagtcgat
     3241 gttagtcaag caggagttgt gactgttaaa ggtacagcaa atggacgtga ggtagaggct
     3301 catgtcgagg ttctagcaat tgcgaaagaa ttaccaacag ttaaacgtat tgctccaaat
     3361 actgacttga attctgtaga caaatctgtt tcttatgttt tgactgatgg aagtgtacaa
     3421 gagtacgaag tagatagctg ggagattacg gaagtagata aagccaaact ttcagtagct
     3481 ggatcacgta ttcaaatgac tggtcagtta gctggagaaa ctattcatgc aacccttgtg
     3541 gtagaagaag gaaatgctgc agcacctgta gtgccaactg ttactgttgg aggtgaggca
     3601 gtaacaggtc ttactagtcg acaaccaatg caatatcgta ctctatctta tggtgcccaa
     3661 ctgccagaag tcacagcaag tgctgaaaat gctgatgtga cagttcttca agcaagcgca
     3721 gcaaacggca tgcgtgcgag catctttatt cagcctaaag atggtggtcc tcttcaaacc
     3781 tatgcaattc aattcctaga agaagcacca aaaattgctc acttgagttt gcaagtggaa
     3841 aaagctgaca gtctcaaaga agaccaaact gtcaaattgt cggttcgagc tcactatcaa
     3901 gatggaacgc aagctgtatt accagctgac aaagtaacct tctctacaag tggtgaaggg
     3961 gaagtcgcaa ttcgtaaagg aatgcttgag ttgcataagc caggagcagt cactctcaaa
     4021 gcggaatatg agggagctaa aggccaagtt gaactcacta tccaagccaa tactgagaag
     4081 aagattgcgc aatctatccg tcctgtaaat gtagtcacag atttgcatca aaaacctact
     4141 cttccaacaa cagtaacggt tgagtatgac aaaggtttcc ctaaagctca caaagtcact
     4201 tggcaagcta ttccgaaaga aaaactagac tcctatcaaa catttgaagt actaggtaaa
     4261 gttgaaggaa ttgaccttga agcgcgtgca aaagtctctg tagaaggtat cgtttcagtt
     4321 gaagaagtca gtgtgacaac tccaatcgca gaagcaccac aattaccaga aagcgttcgg
     4381 acatatgatt caaatggtca cgtttcatca gctaaggttg catgggatgc gattcgtata
     4441 gagcaatacg ctaaggaagg tgtctttaca gttaatggtc gcttagaaga tactcaatta
     4501 acaactaaac ttcatgttcg cgtatctgct caaactgagc aaggtgcaaa catttctgac
     4561 caatggaccg gttcagaatt gccacttgcc tttgcttcag attcaaatcc aagcgaccca
     4621 gtttcaaatg ttaatgacaa gctcatttcc tacaataacc aaccagccaa tcgttggaca
     4681 aactggaatc gtactaatcc agaagcttca gtcggtgttc tgtttggaga ttcaggtatc
     4741 ttgagcaaac gctccgttga taatctaagt gtcggattcc acgaagacca tggagttggt
     4801 gcaccgaagt cttatgtgat tgagtattat gttggtaaga ctgtcccaac agctcctaaa
     4861 aaccctagtt ttgttggtaa tgaggaccat gtctttaatg attctgccaa ctggaaacca
     4921 gttactaatc taaaagcccc tgctcaactc aaggctggag aaatgaacca ctttagcttt
     4981 gataaagttg aaacctatgc tgttcgtatt cgcatggtta aagcagataa caagcgtgga
     5041 acgtctatca cagaggtaca aatctttgcg aaacaagttg cggcagccaa acaaggacaa
     5101 ataagaatcc aagttgacgg caaagactta gcaaacttca accctgattt gacagactac
     5161 taccttgagt ctgtagatgg aaaagttccg gcagtcacag caagtgttag caacaatggt
     5221 ctcgctaccg tcgttccaag cgttcgtgaa ggtgagccag ttcgtgtcat cgcgaaagct
     5281 gaaaatggcg acatcttagg agaataccgt ctgcacttca ctaaggataa gaacttactt
     5341 tctcataaac cagttgctgc ggttaaacaa gctcgcttgc tacaagtagg tcaagcactt
     5401 gaattgccga ctaaggttcc agtttacttc acaggtaaag acggctacga aacaaaagac
     5461 ctgacagttg aatgggaaga agttccagca gaaaatctga caaaagcagg tcaatttacc
     5521 gttcgaggcc atgtccttgg tagtgatctt gttgctgagg tcactgtacg agtgacagac
     5581 aaactaggcg aagctctttc agataaccct aactatgatg aaaacagtaa ccaggccttt
     5641 gcttcagcaa ccaatgatat tgacaaaaac tctcatgacc gcgttgacta tctcaatgac
     5701 ggagatcatt cagaaaatcg tcgttggaca aactggtctc caacaccatc ttctaatcca
     5761 gaagtatcag cgggtgtgat cttccgtgaa aatggtaaga ttgtagaacg gactgttgcc
     5821 caagccaaac ttcgtttctt tgcagatagt ggtacggatg caccaactaa actcgtttta
     5881 gaacgctatg tcggtccaga gtttgaagtg ccaacctact attcaaacta ccaagcctac
     5941 gacgcagacc atccattcaa caatccagaa aattgggaag ctgtgcctta tcgtgcggat
     6001 aaagacatcg cagctggtga tgaaatcaac gtaacattta aagctgtcaa agccaaagct
     6061 atgagatggc gtatggagcg taaagctgac aagagcggtg ttgcgatgat tgagatgacc
     6121 ttccttgcac cgagtgaatt gcctcaagaa agtacgcaat cgaagattct tgtagatgga
     6181 aaagaacttg ctgatttcgc tgaaaatcgt caagactatc aaattaccta taaaggtcaa
     6241 cggccaaaag tctcagttga agaaaacaat caagtagctt caactgtggt agatagtgga
     6301 gaagatagcc ttccagtact tgttcgcctc gtttcagaaa gtggaaaaca agtcaaggaa
     6361 taccgtatcc agttgactaa ggaaaaacca gtttctgctg tacaagaaga tcttccaaaa
     6421 ctcgaatttg ttgaaaaaga tttggcctac aagacagttg agaaaaaaga ttcaacactg
     6481 tatctaggtg aaactcgtgt agaacaagaa ggaaaaactg gtaaagaacg tatctttaca
     6541 gcgattaatc ctgatggaag taaggaagaa aaactccgtg aagtggtaga agctccgaca
     6601 gaccgcatag tcttggttgg aaccaaacca gtagctcaag aagctaaaaa accacaagtg
     6661 tcagaaaaag cagatacaaa accaattgat tcaagtgaag ctagtcaaac taataaagcc
     6721 cagttaccaa atacaggtag tgcggcaagc caagcagcag tagcagcagg tttagctctt
     6781 ctaggtttga gtgcaggatt agtagttact aaaggtaaaa aagaagacta gaaattttga
     6841 taccttcttt atcattaaag accaaagaaa aactcttgtc ctaaatagaa gataaagagt
     6901 ctggaacaaa atagttctca accacaaaaa agctagatat ttccaattgc ggaactctag
     6961 ctttttaatt ttgagtcgtt ctcttattgt atagtagatt gaaactagaa tagtacactt
     7021 ctacttctaa aacattgtta gaaatcaatt tgactgtcct tatcgatttg tcctgttctt
     7081 atttcatttc actat
//
LOCUS       DAVBHS010000103         6978 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVBHS010000103 DAVBHS010000000
VERSION     DAVBHS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.103"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(38..2035)
                     /gene="metG"
                     /locus_tag="VQ651_001549"
     CDS             complement(38..2035)
                     /gene="metG"
                     /locus_tag="VQ651_001549"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET2754523.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEYDGKLTLLTVDPAVPNGSVIG"
     gene            complement(2159..3418)
                     /locus_tag="VQ651_001550"
     CDS             complement(2159..3418)
                     /locus_tag="VQ651_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2754524.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKKSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(3420..4121)
                     /locus_tag="VQ651_001551"
     CDS             complement(3420..4121)
                     /locus_tag="VQ651_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754525.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(4105..5304)
                     /locus_tag="VQ651_001552"
     CDS             complement(4105..5304)
                     /locus_tag="VQ651_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET2754526.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILIPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            5590..6936
                     /gene="gor"
                     /locus_tag="VQ651_001553"
     CDS             5590..6936
                     /gene="gor"
                     /locus_tag="VQ651_001553"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HET2754527.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
BASE COUNT         2022 a         1549 c         1322 g         2085 t
ORIGIN      
        1 aaaaaacaga actagtctga actagtcctg tctactttta cccaatcaca cttccatttg
       61 gtacagctgg atcaactgtg agaagggtta atttgccatc atattcagct gatagaatca
      121 taccctggct gacatatttt ttcatcattt tacgtggttt gaggttagca acgatttgaa
      181 ctttcttgcc gaccaattct tgttcatttg gatagtattt tgcaattcct gaaagaatct
      241 gacgatcttc tccatcacca gcatccaagc ggaattgaag caacttatct gaaccttcta
      301 ctttagacac ttctttgact tctgcgacac ggatttcaac cttgtcaaag tcttcaaact
      361 tgatttcatc cttgtttagt ttgagctcaa cttcgtccgg attccattct ttttcgactg
      421 ctggtttatt gccttccatt tgttccttga tataggcgat ttcttcttcc atatttagac
      481 gtggaaagat aggtgttcct ttggcaacta cagtcacatc tgctgggaag tcagccaaac
      541 tcaagttttc aagactagaa acttcttcca aaccaagttg agtcaaaact gcacgactag
      601 tttccatcat aaatggttca atcaagtgag caactacacg aatgctggct gccaagtggc
      661 tcatgacact tgccaattgg tcacgaagag cttcatcctt ggccaagacc catggtgcgg
      721 tctcatcgat gtatttattg gtacgagaga tcagagtcca gactgcttca agcgcacgtg
      781 gatagtcaac tgcttccatg tgtgtatgga agtctgcgat tgattgttct gcaacctcag
      841 caagaacatg atcaaattca gtcacacctt ctacataggc agggatttgt ccatcaaagt
      901 acttattaat catggaaacc gtacggttaa ggaggttccc aaggtcatta gccaattcat
      961 agttgatacg gccgacatag tcttcaggag taaaggttcc gtctgaacca actggaaggt
     1021 tacgcatgag gtagtaacga agtggatcta gtccataacg ctctaccaac atttcagggt
     1081 aaacgacatt cccttttgac ttagacattt ttccgtcttt catgacaaac caaccatggg
     1141 caatcaaacg atcaggtaat ttaacatcca acatcataag aaggattggc cagtagatag
     1201 agtggaagcg aaggatatct tttcctacca tatggaagac tgttccattc cagaacttgt
     1261 caaagttacc atgttcgtct tgagcgtagc caagagctgt cgcatagtta agaagggcat
     1321 caatccaaac gtagacaacg tgttttggat ttgatgggac aggcactccc catgtaaagg
     1381 ttgtacgaga taccgccaaa tcttccaagc ctggctcgat gaagttgcgt agcatttcat
     1441 taaggcgacc atctggcgtg ataaattcag gatgagcttt gaaaaattcg accaaacggt
     1501 cttggtattt gctaaggcga aggaagtatg attcttcaga aacccattca acctcatgac
     1561 ctgatggagc aataccacca gtcacatttc cagcttcatc acggaaaact tctgccagct
     1621 ggctttctgt aaagaattct tcgtctgata ctgaatacca accagagtat tcacccaagt
     1681 agatatcatc ttgagcaagt aagcgttcaa agacctgtgc gacaactttt tcatggtagt
     1741 catcggttgt acggataaat ttatcgtatg agatatctag taattgccag agttctttaa
     1801 ctccaaccgc cattccatca acataggctt gaggtgtaat accagcttct tccgctttct
     1861 gctggatttt ctgaccatgt tcatcaagac ctgtcagata aaatacatcg tagcccatca
     1921 ggcgtttgta acgtgctagg acatcacatg cgatagttgt gtaggcagaa ccgatatgaa
     1981 gtttcccaga tggatagtaa atcggcgttg taatataaaa atttttttca gacataattt
     2041 ttcctttcca ggcaaatgaa acctgttttt ctaacacttc attatatcac atttttaatg
     2101 aatttcgata gggaaatcca tacaaaaaca agatagacga gtgtccatct tgttgatctc
     2161 attcataacg aagggcttca attggatcaa gtttcgatgc cttgttggct ggcaagactc
     2221 caaaaatcat accaacacta gccgaaactg caagactaaa tagggcaact gggattgata
     2281 ctccaacttc tataccttct attaaacctt gcagtaacaa acctgctaag gcagttaaac
     2341 cacttgcaat tgtcaagcca attaagccac ctaacaaggt caaaatcatg gattcaatca
     2401 aaaactgaat taaaatattg gcacgtgttg cacccaaagc tttacgaaga ccaatctcac
     2461 gagtgcgctc tgtcaccgaa accagcatga tgttcatgac accagttcct ccaacaaaga
     2521 gagaaatccc tgcgatggaa ctaataatcg tcgtcataaa actaaacgat tgttgaattt
     2581 ctgcaaatac aacggactca tctgccacct ggtattctcc ctgttgtaag cctgcaagct
     2641 ctgtcatttt tcgtgccagt tctggaccca gagttggggt taaactggta tcattcactc
     2701 gaaagacaat actagctatt tcatctatat taaaattcgc agcaagggag atattggtag
     2761 taataggcaa gccaccaaac ccatatattt ttgacttttt agcctccgga ctagtataaa
     2821 ccccaatgac ccggtaacta aatccattga cttctacaac cttgttaata gcctcttgag
     2881 gagattcaaa taaactaatg gacaattcct catctagcaa aataacactt gcaaactctt
     2941 tgaaatcttg ctctctcaga ctacgacctg caataatttc attcttaaca gcgtccatgt
     3001 aagttctgtt tccacctgtc aaattagcat tctcaacctt tttatcttga taggtcaaga
     3061 tggcattcgt tgaattggtt acatagtaac tatccactcc cttcagttta gctgcctctt
     3121 gaacccagga ttcttgcggt tttggcggtt caacaggaac ttcctcttcc tttccagaaa
     3181 ccgtaaaagc tgattgtttc tgagtaaaag acccatcttt acttttttta ggagagaaaa
     3241 agacgctaat atttttctga gatttagtca tatctttatt gacttgacga gatagggaat
     3301 cacccaaagc cataatcaca acaactgatg aaacaccgat aataatccca atcatagtaa
     3361 gcaaagaacg catcttgtga gccatgatag atgaaaaggc aaatttcaga ttctgcatct
     3421 tagttttcct cctttcctaa ctgagcactg tcagacgaaa tgaccccatc ccgaatgaca
     3481 atctgacgtt tggcataggc agcaatctca ggctcatgcg ttaccatgat aatggttttt
     3541 ccttctttat tcaaatcaac caataattgc ataatttggt tacctgtttt ggtatccaag
     3601 gctcctgtcg gttcatccgc taggataata gaaggattgt ttaccaaggc acgcgcaatg
     3661 gctacacgtt gcttttgacc accagataat tctgaaggta aatggtgact acgttctgtc
     3721 aattcaacct tgtctaaata ttcctcagcc aacttgcgac gttttgaaga cgaaactcct
     3781 gcgtaaatca agggcaattc tacattttgc agagcattga gcttcgatag aagaaagaac
     3841 tgctgaaaga caaaaccgat ttgttggtta cggaccttag ctagttgttt ttcaccaagc
     3901 ccagccactt cttgaccttc aagataatat tctccactgg ttggtgtatc caacatgcca
     3961 atcgtattca tcagagtgga cttaccagac ccagatggtc ccatgatggc tacaaattca
     4021 ccctcattca cttctagatt gatatttttg agaacctgca gttcttggtc accattacgg
     4081 taacttctga agatattttt tagactaatt agttgcttca tcagccttca cctcttttcc
     4141 ttcttccaag gaagatgttg gattactgat gaccttagca ccgttcgtta aaccagaagt
     4201 gatttcttga ttttctgcgt cagcatttcc caatgaaacc tcaacttttt tagccttttg
     4261 ttgttcatcc acaatccaga cataattttt actatcatcc attactagac tgctaacagg
     4321 aataagaata gccttagttt tgcttttaac ctcaatgttg acagaaaaac cttgtttcaa
     4381 atcaccaacc tcgcctgtca catcaatagt ataagggtat ttagaacctg tattattccc
     4441 ggctgctgga ctagctgctt caccattgtt tttaggatag tcagaaatat agcttaattt
     4501 cccagtccat tttttatcag gatacacttt agaagtaaag cttacttctt gacctacaga
     4561 aaggttggct agattgtact cagacaattc tcccttgact tgtaaatttt cattgctgac
     4621 aatatgaacc ataacttgac tcgcccctgt tggagattta gaaacattgc tattgacttc
     4681 gactacagtt ccctctaggg tactgagaac agttgttgca tccaattgac tttgagcctt
     4741 gcttaattgc gctgcagcat ctgcacgcgc atcacgggca tcacccaatt gagcatcaat
     4801 agaagcaaca gaatttccag ccactggagt tgggctttgc accgttgcat cttctcctcc
     4861 tactggcgct ggtaactgtg gagcctgagc tgaagcggct tcatttcgtg cttgattgag
     4921 ttcattgata tgacgatctg ccttagctac tgctcgacta gctgaatcat aggccgcctg
     4981 cgcttctgaa ctactgtact tgactaaagc ctgcccttcg ctgaccttat cgcccacaga
     5041 aacaaggatt tcatctaaat cacccttact agcatcaaaa taaacatatt gttcattttt
     5101 tgctgttact gtccctgaca ataaaacaga ggatgccacg cttccttcct tggcaacaac
     5161 aagatgagta ggctcatctt ttacagcagt ctgagaaggt tgtctaaaaa gtaaaatccc
     5221 cccggcaccc aatacaacta cactcgcagc accgattgct gcatacagtt gccacttttt
     5281 agctttacca ttctttttct tcataatgaa actccttttc ttttttacaa tactttgcta
     5341 ttataccaaa tttccctcca gcaaacaatg cagttcagat ggaaataatg gcagttcagg
     5401 attaaacaat cgttcggaat tttgcttttc tattgtacta gatatataat acactatctt
     5461 gacttatttg gcaagctttt ttgcaatttt ttgtgcgtag cagatttttg caatcaaatc
     5521 aaaaaaagaa taaaatataa ctatcaaaaa agacactcta ctatttaaaa gattacaaag
     5581 gagttttcaa tgagagaata tgatatcatt gctatcggtg gaggtagcgg aggaatcgct
     5641 accatgaacc gtgctggtga acatggagcc aaagcagccg ttattgagga aaagaaatta
     5701 ggtggaacct gtgtcaacgt cggttgtgtt cctaaaaaaa tcatgtggta cggggcgcaa
     5761 atcgctgaga ctttccatca atttggagaa gactacggct ttaagactac tgatcttaac
     5821 tttgactttg caaccctacg tcgcaatcgt gaagcctaca ttgatcgcgc tcgttcttct
     5881 tatgatggta gttttaaacg caacggtgta gacttgattg aaggtcatgc tgaatttgta
     5941 gattctcata ctgtaagcgt aaatggtgaa ctgattcgtg ctaaacatat cgtgattgct
     6001 acaggtgccc atccaagtat tcctaatatt cctggtgctg agctaggtgg ctcttctgat
     6061 gatgtatttg cctgggaaga acttccagag tcaattgcca ttctaggcgc tggttatatc
     6121 gccgttgaat tagctggcgt actccacact tttggtgtca agacagatct ctttgttcgc
     6181 cgcgatcgtc ctttacgtgg ttttgattcc tacatcgttg aaggtttggt caaggaaatg
     6241 gaaagaacaa acttaccact tcacactcac aaagtccctg tcaagttaga aaaaactact
     6301 gacggcatta ccattcattt cgaagatggt actagtcaca cagctagcca agttatctgg
     6361 gctacaggtc gccgtccaaa cgttaagggc ttgcaacttg aaaaagctgg agtgactctg
     6421 aacgaacgtg gctttatcca agtggatgaa taccaaaata ctgttgttga gggaatctat
     6481 gctctaggtg atgtaacggg cgagaaagaa ctgactccag ttgcaatcaa ggccggacgt
     6541 accctatctg aacgtctctt taacggaaaa actactgcaa aaatggatta ctcaactatt
     6601 ccaactgttg tcttttcaca ccctgctatc ggaactgttg gtttgacaga agagcaagct
     6661 attaaagaat acggtcaaga ccaaatcaag gtttataaat caagctttgc atctatgtac
     6721 tctgcttgca ctcgcaaccg tcaagaatcc cgtttcaaac tcataacagc tggttcagaa
     6781 gaaaaagttg tcggacttca tggaattggc tacggcgttg atgaaatgat tcagggattt
     6841 gccgttgcta tcaaaatggg agcaaccaag gctgactttg atgcaactgt ggcaattcac
     6901 ccaactgcat ctgaagaatt tgtaaccatg cgttaatcga aacaaaagaa gctgatataa
     6961 atatgtctgc ttcttttt
//
LOCUS       DAVBHS010000104         6912 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVBHS010000104 DAVBHS010000000
VERSION     DAVBHS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.104"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            226..6657
                     /locus_tag="VQ651_001554"
     CDS             226..6657
                     /locus_tag="VQ651_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET2754528.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VVDKETENKEKHEDIHNAIETSQDFKEKKTAVIEGKEVVSKNPVTDNNTSNEEARIKE
                     DSNQSQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPYGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANHADEKG
                     MISYYLVDPDKDASKYEKLGEISEDKLKNAKSPEENTNNNQAKDEDSKPDEKSSVEGE
                     ASLEINKTISTIREFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIAY
                     DDGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIEI
                     KATKYDSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKDDNQIKAETKIRMPEKNKET
                     KAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGY
                     ALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQ
                     AVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKS
                     NVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWE
                     LRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDG
                     NVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRN
                     NGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTG
                     EVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKD
                     AGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQ
                     REDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVG
                     IFLGLKKKNQD"
BASE COUNT         2742 a          930 c         1330 g         1910 t
ORIGIN      
        1 taatcttatt ttttaacgtg tgctaaaaaa taaattaatg catgttaatt tttcttgaat
       61 taaatttttt taaatgatat actgttttta tggagagata acaattatat ataaatgtac
      121 tactctattt cctagataat acttagtaaa actttttata acaaaggcta gcaaagttaa
      181 agtttattat ctattggaag ttaaataaat atgtaaggaa ttaaaatgaa aaaaagtaca
      241 gtattgtcat taaccacagc tgcagttatt ttagcagcct atgcccctaa tgaggtagtc
      301 ttagcagaca catctagctc tgaagatgct ttaaacatct ctgataaaga aaaagtagta
      361 gtagataagg aaacagaaaa taaagagaaa catgaagata ttcataatgc tatagaaact
      421 tcacaggatt ttaaagagaa gaaaacagca gtcattgagg gaaaagaagt tgttagtaaa
      481 aatcctgtga cagacaataa cactagcaat gaagaagcaa gaatcaaaga agattccaat
      541 caatcccaag gagatcatac ggactcattt gtgaataaaa acacagaaaa tcccaaaaaa
      601 gaagataaag ttgtctatat tgctgaattt aaagataaag aatctggaga aaaagcaatc
      661 aagcaactat ccaatcttaa gaatacaaaa gttttatata cttatgatag aatttttaac
      721 ggtagtgcca tagaaacaac tccagataac ttggacaaaa ttaaacaaat agaaggtatt
      781 tcatcggttg aaagggcaca aaaagtccaa cccatgatga atcatgccag aaaggaaatt
      841 ggagttgagg aagctattga ttacctaaag tctatcaatg ctccgtttgg gaaaaatttt
      901 gatggtagag gtatggtcat ttcaaatatc gatactggaa cagattatag acataaggct
      961 atgagaatcg atgatgatgc caaagcctca atgagattta aaaaagaaga cttaaaagga
     1021 actgataaaa attattggtt gagtgataaa atccctcatg cgttcaatta ttataatggt
     1081 ggcaaaatca ctgtagaaaa atatgatgat ggaagggatt attttgaccc acatgggatg
     1141 catattgcag ggattcttgc tggaaatgat actgaacaag atatcaaaaa ctttaacggc
     1201 atagatggaa ttgcacctaa tgcacaaatt ttctcttaca aaatgtattc tgacgcagga
     1261 tctgggtttg cgggtgatga aacaatgttt catgctattg aagattctat caaacacaac
     1321 gttgatgttg tttcggtatc atctggtttt acaggaacag gtcttgtagg tgagaaatat
     1381 tggcaagcta ttcgggcatt aagaaaagca ggcattccaa tggttgtcgc tacgggtaac
     1441 tatgcgactt ctgcttcaag ttcttcatgg gatttagtag caaataatca tctgaaaatg
     1501 accgacactg gaaatgtaac acgaactgca gcacatgaag atgcgatagc ggtcgcttct
     1561 gctaaaaatc aaacagttga gtttgataaa gttaacatag gtggagaaag ttttaaatac
     1621 agaaatatag gggccttttt cgataagaat aaaatcacaa caaatgaaga tggaacaaaa
     1681 gctcctagta aattaaaatt tgtatatata ggcaaggggc aagaccaaga tttgataggt
     1741 ttggatctta ggggcaaaat tgcagtaatg gatagaattt atacaaagga tttaaaaaat
     1801 gcttttaaaa aagctatgga taagggtgca cgcgccatta tggttgtaaa tactgtaaat
     1861 tactacaata gagataattg gacagagctt ccagctatgg gatatgaagc ggatgaaggt
     1921 actaaaagtc aagtgttttc aatttcagga gatgatggtg taaagctatg gaacatgatt
     1981 aatcctaata aaaaaactga agtcaaaaga aataataaag aagattttaa agataaattg
     2041 gagcaatact atccaattga tatggaaagt tttaattcca acaaaccgaa tgtaggtgac
     2101 gaaaaagaga ttgactttaa gtttgcacct gacacagaca aagaactcta taaagaagat
     2161 atcatcgttc cagcaggatc tacatcttgg gggccaagaa tagatttact tttaaaaccc
     2221 gatgtttcag cacctggtaa aaatattaaa tccacgctta atgttattaa tggcaaatca
     2281 acttatggct atatgtcagg aactagtatg gcgactccaa tcgtggcagc ttctactgtt
     2341 ttgattagac cgaaattaaa ggaaatgctt gaaagacctg tattgaaaaa tcttaaggga
     2401 gatgacaaaa tagatcttac aagtcttaca aaaattgccc tacaaaatac tgcgcgacct
     2461 atgatggatg caacttcttg gaaagaaaaa agtcaatact ttgcatcacc tagacaacag
     2521 ggagcaggcc taattaatgt ggccaatgct ttgagaaatg aagttgtagc aactttcaaa
     2581 aacacggatt ctaaaggttt ggtaaactca tatggttcca tttctcttaa agaaataaaa
     2641 ggtgataaaa aatactttac aatcaagctt cacaatacat caaacagacc tttgactttt
     2701 aaagtttcag catcagcgat aactacagat tctctaactg acagattaaa acttgatgaa
     2761 acatataaag atgaaaaatc tccatatggt aagcaaattg ttccagaaat tcacccagaa
     2821 aaagtcaaag gagcaaatat cacatttgag catgatactt tcactatagg cgcaaattct
     2881 agctttgatt tgaatgcggt tataaatgtt ggagaggcca aaaacaaaaa taaatttgta
     2941 gaatcattta ttcattttga gtcagtggaa gaaatggaag ctctaaactc caacgggaag
     3001 aaaataaact tccaaccttc tttgtcgatg cctctaatgg gatttgctgg gaattggaac
     3061 cacgaaccaa tccttgataa atgggcttgg gaagaagggt caagatcaaa aacactggga
     3121 ggttatgatg atgatggtaa accgaaaatt ccaggaacct taaataaggg aattggtgga
     3181 gaacatggta tagataaatt taatccagca ggagttatac aaaatagaaa agataaaaat
     3241 acaacatccc tggatcaaaa tccagaatta tttgctttca ataaccaagg gatcaacgct
     3301 ccatcatcaa gtggttctaa gattgctaac atttatcctt tagattcaaa tggaaatcct
     3361 caagatgctc aacttgaaag aggattaaca ccttctccac ttgtattaag aagtgcagaa
     3421 gaaggattga tttcaatagt aaatacaaat aaagagggag aaaatcaaag agacttaaaa
     3481 gtcatttcga gagaacactt tattagagga attttaaatt ctaaaagcaa tgatgcaaag
     3541 ggaatcaaat catctaaact aaaagtttgg ggtgacttga agtgggatgg actcatctat
     3601 aatcctagag gtagagaaga aaatgcacca gaaagtaagg ataatcaaga tcctgctact
     3661 aagataagag gtcaatttga accgattgcg gaaggtcaat atttctataa atttaaatat
     3721 agattaacta aagattaccc atggcaggtt tcctatattc ctgtaaaaat tgataacacc
     3781 gcccctaaga ttgtttcggt tgatttttca aatcctgaaa aaattaagtt gattacaaag
     3841 gatacttatc ataaggtaaa agatcagtat aagaatgaaa cgctatttgc gagagatcaa
     3901 aaagaacatc ctgaaaaatt tgacgagatt gcgaacgaag tttggtatgc tggcgccgct
     3961 cttgttaatg aagatggaga ggttgaaaaa aatcttgaag taacttacgc aggtgagggt
     4021 caaggaagaa atagaaaact tgataaagac ggaaatacca tttatgaaat taaaggtgcg
     4081 ggagatttaa ggggaaaaat cattgaagtc attgcattag atggttctag caatttcaca
     4141 aagattcata gaattaaatt tgctaatcat gctgatgaaa aggggatgat ttcttattac
     4201 ctagtagatc ctgacaaaga tgcttctaaa tatgaaaaac ttggtgaaat ttctgaagat
     4261 aaactcaaaa atgcaaagag cccagaggaa aataccaata ataatcaagc taaggatgaa
     4321 gattcaaaac cagatgaaaa aagttcagtt gagggagaag ctagccttga aataaataaa
     4381 actatttcaa caattagaga gtttgaaaat aaagacctaa agaaactcat taaaaagaaa
     4441 tttagagaag ttgatgattt tacaagtgaa actggtaaga gaatagagga atacgattat
     4501 aaatacgatg ataagggaaa tattattgct tatgacgatg gtagtgcctt acaatatgaa
     4561 actgaaaaac ttgacgaaat aaaatcaaaa atttatggtg ttctaagccc atctaaagat
     4621 ggacactttg aaattcttgg aaagataagc aatgtttcta aaaatgccaa ggtatattat
     4681 ggaaatagct ataaatcgat agaaatcaaa gcgactaagt atgattcaca ttcaaaaacg
     4741 atgatatttg atttatacgc taatattaat gatattgtag atggattagc ttttgctgga
     4801 gatatgagat tatttgttaa agatgacaat caaataaaag ctgaaactaa gattagaatg
     4861 cctgaaaaaa ataaggaaac taaagcagaa tatccctatg tatcaagtta tgggaatgta
     4921 atcgaattag gagaaggaga tctttcaaag aacaaaccag acaatttaac taaaatggaa
     4981 tctggtaaaa tctattctga ttcagaaaaa caacaatatc tgttaaagga taacatcatt
     5041 ctaagaaaag gctatgcact aaaagtgact acctataatc ctggaaaaac ggatatgtta
     5101 gaaggaaatg gagtctatag caaggaagat atagcaaaaa tacaaaaggc caatcctaat
     5161 ctaagagtcc tttcagaaac aacaatttat gctgatagta gaaatgttga agatggaaga
     5221 agtacccaag ctgtattaat gtcggctttg gacggcttta acattataag gtatcaagtg
     5281 tttacattta aaatgaacga taaaggggaa gctatcgata aagacggaaa tcttgtgaca
     5341 gattcttcta aacttgtatt atttggtaag gatgataaag aatacactgg agaggataag
     5401 tccaatgtag aagctataaa agaagatggc tccatgttat ttattgatac caaaccagta
     5461 aacctttcaa tggataagaa ctactttaat ccatctaaat ctaataaaat ttatgtacga
     5521 aatccagaat tttatttaag aggtaagatt tctgataagg gtggttttaa ctgggaattg
     5581 agagttaatg aatcggttgt agataattat ttaatctacg gagatttaca cattgataac
     5641 actagagatt ttaatattaa gctgaatgtt aaagacggtg acatcatgga ctggggaatg
     5701 aaagactata aagcaaacgg atttccagat aaggtaacag atatggatgg aaatgtttat
     5761 cttcaaactg gctatagcga tttgaatgct aaagcagttg gagtccacta tcagttttta
     5821 tatgataatg ttaaacccga agtaaacatt gatcctaagg gaaatactag tatcgaatat
     5881 gctgatggaa aatctgtagt ctttaacatc aatgataaaa gaaataatgg attcgatggt
     5941 gagattcaag aacaacatat ttatgtaaat ggaaaagaat atacatcatt tgatgatatt
     6001 aaacaaataa cagacaagac actaaacatt aagattgttg taaaagattt tgcaagaaat
     6061 acaaccgtaa aagaattcat tttaaataaa gatacgggag aggtaagtga attaaaacct
     6121 catagggtaa ctgtgaccat tcaaaatgga aaagaaatga gttcaacgat agtgtcggaa
     6181 gaagatttta ttttacctgt ttataagggt gaattagaaa aaggatacca atttgatggt
     6241 tgggaaattt ctggtttcga aggtaaaaaa gacgctggct atgttattaa tctatcaaaa
     6301 gataccttta taaaacctgt attcaagaaa atagaggaga aaaaggagga agaaaataaa
     6361 cctacttttg atgtatcgaa aaagaaagat aacccacaag taaaccatag tcaattaaat
     6421 gaaagtcaca gaaaagagga tttacaaaga gaagatcatt cacaaaaatc tgattcaact
     6481 aaggatgtta cagctacagt tcttgataaa aacaatatca gtagtaaatc aactactaac
     6541 aatcctaata agttgccaaa aactggaaca gcaagcggag cccagacact attagctgcc
     6601 ggaataatgt ttatagtagg aatttttctt ggattgaaga aaaaaaatca agattaagac
     6661 aaaagctata gaaaaaaatg gtttatgtac tgagattaga tagtgaggtg atgacatagt
     6721 tttgtgaaaa cagccattta taactcaatt atttagttta cttcactatt gatactattt
     6781 ggagttatta atggacttag tttagataac taatgaattg attgaaaggg ttagtattga
     6841 caatattggt catattgatt agaaaataga gtctatcaaa atttaaaggc taatagaggt
     6901 gatgagacaa tt
//
LOCUS       DAVBHS010000105         6817 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVBHS010000105 DAVBHS010000000
VERSION     DAVBHS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.105"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(70..915)
                     /locus_tag="VQ651_001555"
     CDS             complement(70..915)
                     /locus_tag="VQ651_001555"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET2754529.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            1170..2189
                     /locus_tag="VQ651_001556"
     CDS             1170..2189
                     /locus_tag="VQ651_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET2754530.1"
                     /translation="MLYVGIDIAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALENTGHYAFNLLNFLHEQGYKVYTYTPLLIKEFAKSLSLRKTKTNKK
                     DAHGIALKLLSDPNREQFQHDNRQVELKILARHIHRLKKKQSDWKVQYTRCLDIIFPE
                     LDKIVEKHSEYTYQLLTCYPNPQKRLEAGFDKLIEIKRLTASKIQDILSVAPRSIGTT
                     SPAREFEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGNRLEAVILAEIRNIHAF
                     DNPAQLQAFAGLDSSIYQSGQIDLAGRMIKRGSPHLRWALIQAAKACPRFSPAFKAYL
                     KTKLE"
     gene            complement(2490..4379)
                     /locus_tag="VQ651_001557"
     CDS             complement(2490..4379)
                     /locus_tag="VQ651_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET2754531.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDQAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSLFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            4517..6082
                     /locus_tag="VQ651_001558"
     CDS             4517..6082
                     /locus_tag="VQ651_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET2754532.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKKPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMCKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            6082..>6817
                     /locus_tag="VQ651_001559"
     CDS             6082..>6817
                     /locus_tag="VQ651_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2754533.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEEIAKENLEKVGMGERYWQARPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYL"
BASE COUNT         2151 a         1534 c         1250 g         1882 t
ORIGIN      
        1 agctgacgcg gtttgaagag attttcgaag agtataaaac cttgaagagt tgccccttca
       61 aggtttttct tatacaaata aacggacaaa actgtaaagt agcaaaacac taccaagcac
      121 gatacggtat ttaccaaaaa gggtgaagtc gtgttttttc acatagctgg tcaagaagcg
      181 aatagccacc atgctgaccg caaaagctac tcccatcgcg accaagagca aaaacaattg
      241 cccaaagctc aagagttctc cggctttcac aaatttgaaa atctttaagg cactagctcc
      301 aaacataaca ggaatcccaa gatagaaggt aaattctgtc acaactgaac gactggttcc
      361 atttaacaaa ccaccgacaa tcgttgcacc tgaacggcta gtccctggta aaagagcaag
      421 aacttggaag agtccgatat agaaagcggt cgtataagga agcttgtcca actctgttac
      481 acttggctcg atagcacgcg ctttattgcg cttttccaaa tagatgaagg caaccccgta
      541 gataatcaac atgagagcaa ctgaaaccat gttatggaag tgggtatcaa accaatcatc
      601 aaatttaaag acaccaagta aaggtaaagt agcaatcaag accttcaacc atagtctcca
      661 agtcttacga acttcctgtt tgtccttagt cggtttaaaa ggattgagct tgttaaaata
      721 aatcaccata actgctaaaa tagcaccaag ctgaatcacg acattaaaca tggacataaa
      781 ggcttcattt tgattttggt attggatgaa ttcctctgct aaaatcaagt gacctgtact
      841 ggaaatcggc aaccattccg taattccttc aacaatcccg aagaagatag attttaaaat
      901 ttcaataaga tacatagatt actccctata ttataattag cgcagttagc tactgccaaa
      961 gcccaagtgg tatacttgga ataagccact gtggattagt tcatttcctc caatcgcttg
     1021 acgatttccg aaaggctcta accacagctt ttgtttcaat tggtaatgag tttgataacg
     1081 catagacgtt tgatctcgtc taaggtaaca aggcaaaaaa ttcgtggata accaagtaga
     1141 cttgaaatac tatatctgga ggtactccca tgctttatgt gggcattgat atcgctaaaa
     1201 ataaacacga tgttacagcc ttgaatgttc caggaaaaac tgttcttaaa ccactcactt
     1261 tttcaaataa taaagctggt tttgaactct tagatctgtc tcttcgacag ctcaaccaag
     1321 actgtctcat cgctcttgaa aataccggac attatgcctt taatctactg aacttcctgc
     1381 atgaacaagg atataaagtt tacacctaca ctcctttgct catcaaggaa tttgccaaat
     1441 cattatcact tcgcaagact aaaacgaata agaaagacgc tcatggcatc gctcttaaac
     1501 ttctttctga ccccaatcgt gaacaatttc aacacgataa tcggcaagta gaactaaaaa
     1561 tactggctag acatattcat cgtctcaaga aaaaacagtc tgattggaaa gtacaataca
     1621 ctcgttgtct tgatatcatc tttcctgagt tggataaaat cgttgaaaag cattcagaat
     1681 atacctacca actcttgaca tgctacccta atcctcagaa aaggcttgag gcaggatttg
     1741 ataagctgat agaaattaag cgattgaccg cttctaaaat tcaggatatc ctctcagttg
     1801 cacctcgttc tatcggaaca acatctcctg ctcgtgaatt cgaaatcatc aaacattaca
     1861 agaggctcat tgacaaggcg gaaacatgtg tcaatgactt gatggctgag ttcaactcgg
     1921 tcatcacgac ggtcactggg attgggaatc gtttagaggc ggtcatttta gccgagattc
     1981 gaaatattca tgcctttgat aatcctgctc aattacaagc tttcgctgga ctggattctt
     2041 ctatttatca gtcaggtcag attgatttag ctggaagaat gatcaaacgg ggttcccctc
     2101 atctgcggtg ggcactcata caagctgcca aagcatgccc tcgcttttca cctgctttta
     2161 aggcctatct taagactaag ttagaataag gaaaacatta caatgtagcc atcatccacc
     2221 ttgcaaaaaa acttatccga accctgtttt atatcctaaa aaagagctgc catttgacga
     2281 acaaaaagtg atgtaatttc tgaaattaac acaggattac ctttgaaaaa atggactttt
     2341 tcaagggctt tttgccgtgc tctaaaatca tcgccaaatc atcaaattga aataagaaat
     2401 agtttagata aaactattga cttttcttat agtttgtctt ttttctatct tccattatat
     2461 catacttttt cagtctatgg tagctctctt taaaaggcac cgatactgcc gccacctccg
     2521 cctccagaga agccaccacc agaacttcca cttccagaag atacagagta ggtgcttgct
     2581 gtatttgcga cactagcata atggctcatt tgcgcgcttg aatgataaaa cagactgtgc
     2641 cagccataag ctacatagag attgatatct ggattttcca cttgaatctg atgaaccttc
     2701 atcaaatgac taaccttgtc cgcatagcca aataaggtcg catagaccaa gaggcgattc
     2761 cagaccacaa tactttccag ttcagcctga tccaatcgtg caatctcacg caacatattt
     2821 tcaaaactgg tccagagata gtagacctca gctcccgctt catttagaac accatcacga
     2881 ttatctagtc gaagcttcca ataatagaaa acagacaaaa ctaaccctag aaaaccaagt
     2941 attggcaaag ggaggtaaag atagccatga acgtctaaac tgtacaagaa caaaccaaat
     3001 ccgataaata ggggcaggat agtcaaggca cccataccca cttgcaaggc cttttcccca
     3061 ccagttaaag gacgataata atctgggagc ccccagaagg aaactcgttt tctcactcct
     3121 tcttgcatct ggttcaatac ctcttcaaaa gaagatttga gttgaagccc tcttgcttga
     3181 atccgttttt catcagaaac tttggctcta cgataaagac tatcagatac cttgtaatcc
     3241 gcaaacaaat tggaaagagt ttcttctttt ttacctgaaa aagctagatt taggcagtct
     3301 ttctcaaagc ttgacaaacc atcttctttt actagcctca aaccaactgc atctccttct
     3361 gaaatgatag agacattccc acggtctatc acatctagca aggtagcttg aataagttga
     3421 tcaaaggtga attttccagc tcccttgacc aagggactca cttcctccaa ggaggtcgag
     3481 tagactgctt ctgataaaac cataggctct aattccattg gtggttcata gagacgatga
     3541 tttttggcat atttgactga aggagtggtc tttcttctat aaataaaata gaagcagaca
     3601 ctcaataaca aggagataga aaggatcgaa gggaggaccc aagtaacaag ttgtttactc
     3661 tgatcttttt ctctaacaat cgagtcttct atcttattaa actcttctaa acgattccct
     3721 ttcaatccct gatccctagc gctagcaaaa tcggtccgag gccaataggc atgcaactca
     3781 actccacgct tagccggaag attgtctaaa cggatagtat aatcaaggtt actcttttca
     3841 atcgttccct ctctaaaaag tttccctgta tggaaaaaga gtttttcagc tcccttgtct
     3901 ccccttacat gaaattcaaa cttttcaata gactctgaac tatctgtcag aggttgccaa
     3961 tttaattcag cgatatcatc ataaaggaaa agtaaatttt ttaagttcca gacgaggtca
     4021 acttcaacta tgtcgccctc ctgacctgga ttatagactc tcacagtata accatccgct
     4081 tcttctgtta cttcgctagt cacatctgct agttctgcac cgtttttcgc ggcctgaacc
     4141 tttggatgag ggtcaatgtc aaacccgcta ggcatcttac cagcacgtcc aagtcccacg
     4201 atttgtccct taaagtcctc ctcaaactgg taaactatct tctgtctaaa ctctgccgta
     4261 ttatctgcat gaatatacaa atccccttga taagagttta tcttgaaatc aatggcaaaa
     4321 acagagagtg gaagaaggca aaacaagcct aacaccagta agaaaaaagt ttttttcatc
     4381 aactaagccc cctttttatc tttcttatca ttatagcatt ttttcgtaag taaaggctta
     4441 cttatgttga aaaattacgc atttttcgat aaaataggag acagttattt tttaatagat
     4501 cagaatagga gaaatcatga gaaaaatata cttatctatt ttcacaagtc tcttgctgat
     4561 gctaggactt gtcaatgttg ctcaagccga tgaatattta cgcatcggga tggaagcagc
     4621 atatgctccc tttaactgga cccaggatga tgatagcaac ggagctgtca aaatcgatgg
     4681 gaccaatcag tatgccaacg gatacgatgt tcaaatcgcc aagaaaatcg ctaaggactt
     4741 aggtaaaaaa cctttggttg ttaaaaccaa gtgggaaggt ctagtccctg cccttacttc
     4801 tggtaagatt gacatgatta tcgcaggtat gagtccaact gcagaacgca aacaagaaat
     4861 tgccttttcg agcagttact acactagcga accagttttg cttgtcaaaa aagattctgc
     4921 ctacgcaagt gctaaatctt tggatgactt taacggtgca aaaatcactt ctcaacaagg
     4981 ggtctacctc tacgacttga ttgcacaaat cccaggtgct aaaaaagaaa cagccatggg
     5041 agacttcgct caaatgcgac aagctcttga ggctggtgtc attgatgctt atgtttctga
     5101 acgtccagaa gcactgactg ctgaagctgc gaactctaag ttcaagatga ttcaagtaga
     5161 acctggtttc aaaactgggg aagaagatac agctatcgct atcgggcttc gtaaaaatga
     5221 caatcgtatt agccaaatca atgccagcat tgaaaccatt tcaaaagatg accaagttgc
     5281 cttgatggat cgtatgatca aggaacaacc tgccgaagct acaacaactg aagagactag
     5341 cagtagtttc tttagccaag tcgctaaaat tctttctgaa aactggcaac aactcttgcg
     5401 tggtgctggt atcactcttt taatctctat cgtcggaacc atcataggtc tcattattgg
     5461 acttgccatt ggtgtcttcc gtactgctcc tctctctgaa aacaaagcca tttatggcct
     5521 tcaaaaacta gtcggctggg ttctcaatgt ctacattgaa attttccgtg gtacgccaat
     5581 gattgttcaa tcgatggtta tctactatgg aactgcccaa gctttcggta tcaaccttga
     5641 ccgtacacta gctgctatct tcatcgtttc aatcaatacc ggtgcctaca tgactgaaat
     5701 tgtccgtggt ggtatcctgg cagttgacaa gggacaattt gaagctgcga ctgctcttgg
     5761 tatgactcat aaccaaacca tgtgtaagat tgtcctacct caggttgtcc gcaatatcct
     5821 accagcaact ggtaatgaat ttgtcatcaa tatcaaagat acatctgtat tgaacgttat
     5881 ctctgttgtc gaactttatt tctcaggaaa taccgtggca acacaaacct atcaatactt
     5941 ccagacattt acaatcatcg ccgtgattta ctttgtcctc accttcaccg taacacgtat
     6001 cctacgcttt atcgagcgca gaatggacat ggatacctac actacaggtg ctaaccaaat
     6061 gcaaacggag gatttgaaat aatgacacaa gcaatccttg aaattaaaca cctcaaaaaa
     6121 tcctatggac aaaacgaagt gctaaaagac atttctctca ctgtccacaa gggagaagtt
     6181 atttctatca tcggaagctc tggaagtggg aaatcaacct tcctacgctc cattaaccta
     6241 cttgaaacgc caactgatgg acaaatcctt tatcatggac aaaacgtcct cgaaaaaggc
     6301 tatgacctca cgcaataccg tgaaaagtta gggatggttt tccaatcctt taacctcttt
     6361 gaaaatctca acgttcttga aaacacaatc gtcgctcaga caactgtcct taaacgcgaa
     6421 cgcacagaag ctgaagaaat tgccaaagaa aacctggaaa aggtcggcat gggagaacgc
     6481 tactggcaag cgagacccaa acaactctca ggtggtcaaa aacaacgtgt ggccatcgct
     6541 cgtgcccttt caatgaatcc tgacgccatt ctctttgatg aaccaacatc agctctcgat
     6601 ccagaaatgg ttggagaggt cctcaaaatc atgcaagatc tggctcagga aggcttgacc
     6661 atgattgtcg taacccatga aatggaattt gcccgtgatg tctctcaccg tgttatcttt
     6721 atggataagg gtgtgattgc tgaagaaggt aaaccagaag acctcttcac taatcctaaa
     6781 gaagaccgta caaaagaatt tcttcaacgt tatctca
//
LOCUS       DAVBHS010000106         6806 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVBHS010000106 DAVBHS010000000
VERSION     DAVBHS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.106"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            70..771
                     /locus_tag="VQ651_001560"
     CDS             70..771
                     /locus_tag="VQ651_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754534.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            773..3473
                     /locus_tag="VQ651_001561"
                     /pseudo
     CDS             773..3473
                     /locus_tag="VQ651_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            complement(3569..4060)
                     /gene="tpx"
                     /locus_tag="VQ651_001562"
     CDS             complement(3569..4060)
                     /gene="tpx"
                     /locus_tag="VQ651_001562"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET2754535.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(4183..5112)
                     /gene="psaA"
                     /locus_tag="VQ651_001563"
     CDS             complement(4183..5112)
                     /gene="psaA"
                     /locus_tag="VQ651_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HET2754536.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(5144..5992)
                     /locus_tag="VQ651_001564"
     CDS             complement(5144..5992)
                     /locus_tag="VQ651_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET2754537.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(5989..6711)
                     /locus_tag="VQ651_001565"
     CDS             complement(5989..6711)
                     /locus_tag="VQ651_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET2754538.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT         1960 a         1330 c         1534 g         1982 t
ORIGIN      
        1 gatggaagct gacgtggttt gaagagagat tttcgaggag tataatttgt ttgatagagg
       61 gtgggtctga tggcttatat tgagatgaaa cactgttaca agcgttatca ggttggggac
      121 acggagattg tggccaatcg tgatgtgaat tttgagattg aaaaggggga gctggttatt
      181 atccttggtg cttcaggtgc aggcaagtca acagttctta accttcttgg gggaatggat
      241 accaatgatg aaggggaaat ctggattgat ggtgttaata ttgcggatta tagttcccac
      301 cagcgtacca attaccgtag aaatgatgtg gggtttgttt ttcagtttta taatctagtt
      361 tctaatctga cagctaagga aaatgtggaa ctggcttctg aaattgtgac agatgccttg
      421 aatcctgatc aggtcttgac agatgtaggt ctggctcatc gtctcaataa ctttccagcc
      481 cagctttctg gaggggagca acagcgagtc tccattgcac gcgcggtagc caaaaatcct
      541 aaaattctcc tttgtgatga accgactgga gccttggatt atcagacggg caagcaggtt
      601 ttgaaaattc tccaagacat gtctcgtcaa aagggagcga cggtgatcat cgtgactcat
      661 aatggagctt tggcgcccat tgctgatcgc gtgattcaaa tgcacgatgc cagtgtcaag
      721 gatgtggtgc tcaaccagca tcctcaggat attgacagtt tggagtacta gcatgatcaa
      781 gcgaaaaact tattggaagg acttaattca gtccttcaca ggctccaagg ggcgtttttt
      841 atccatcttg atcctgatga tgttgggatc tctagcctta gtaggcctca aagtaaccag
      901 tcccaacatg gaggcgacag ctaatgctta tttaacaact gctcaaacct tggatttggc
      961 agtcatgtct aactatggct tggatcaagc agaccaagaa gaactaaaac agacggaggg
     1021 cgcagaggtc gagtttggct atttgacaga tgtgactatg gataatgggc aggatgccat
     1081 tcggctgtac tccaaaccag agcgaatttc aacctttcag ctaagaaagg gacgacttcc
     1141 tcagtcagac aaggaaatcg ctttggccac tcatttgcaa ggccaataca gcgtgggaca
     1201 ggagattagt tttaaagaaa aagaagaggg tcattcctct ttaaaagacc atacttatac
     1261 cattactggt tttgtggatt cggctgaaat cctctcccag cgagatatgg gctacgcagg
     1321 aagtggaagt gggactctga cagcctatgg ggtgatttta cctagtcagt ttgatcagaa
     1381 agtctacaat atagctcgtt tgaaatatca agatttagca ggtttaaatg ccttttcatc
     1441 agcttatgaa gaaaaatcca agcaacatca ggaagacctt gaacaaactt tatcagataa
     1501 tggcaaggta cgtctgcaac ttttgaaaaa agaaggacaa gagtctctag acaaggggca
     1561 agagaccctt gacaaggctc agactaattt gcaggaaggc aagcgtcgtt tagcagctgc
     1621 tcaagctcgt atacaggctc aagaaagtca actagccttg tttcctcaag ttcagagaga
     1681 gcaggctagt gctcaactta cccaagccaa gcaggaattg ggcaaggaag aggataaact
     1741 aaagcaagct gaacaaaatc tagcccaaga aaaggaaaaa ttagaaaaac atcagcaagt
     1801 cttggacgat ttggcggagc caaggtatca ggtctataat cgtcagacca tgccaggtgg
     1861 tcagggctat cttatgtata gcaatgcttc atccagtatt cgagcagtgg gcaatatctt
     1921 tcctgtggta ctttatgccg tagcatccat ggtgaccttt acgaccatga ctcgctttgt
     1981 agacgaagag cgaactcatg cagggatttt taaggccttg ggttaccgta gtaaggatat
     2041 tatcaccaag tttctccttt atggactagt agctgggact gtcggaacgg ctctaggtag
     2101 tatacttggt cattatttgc tagccagtgt aatttcaagt gtcattacaa aaggcatggt
     2161 ggtgggagaa acccagattc agttctattg gacctatagc ttactagctc ttgtcttgag
     2221 ctggttggcg agtgtgttac cagcctatct ggtggcttgg agggaacttc atgccgaagc
     2281 agcccagctt ctacttccta aacctcctgt caaaggagct aaaatcttat tggagcgtat
     2341 cggttttatc tggcgtcgtc tcagttttac tcataaggta acagcccgca acatctttcg
     2401 ttataagcag agaatgttga tgacaatctt tggtgtggca ggttctgtag ctctgctctt
     2461 tgcaggtttg ggaatccaat cttctgtagc aggagttccg tctaaacagt ttcaacaaat
     2521 ccaacagtat cagatgcttg tctctgaaaa tcctagtgcg accaatcagg acaaggtaga
     2581 gctagcagaa gtgttgaaag ggcaggagat actagcctac cagaaaatct attctaaaac
     2641 gctagacaag gatttcaaag gcaaggctgg tcttcaaaac attactctta tgatgataga
     2701 gaaggaagat ttgactccct ttatccatct tcaacatcat cagcaggagc tgacattaaa
     2761 agatggcatc gttattacag ctaaactcgc ccagctggca ggtgtcaagg ttgggcagac
     2821 tttagaaatt gaaggtaagg aactaaaggt cgctgctatt actgagaact acgttggtca
     2881 ctttatttat atgagtcagg ctagctatga gcaactttac ggacagctac cccaagccaa
     2941 cacttatctg gtctcattaa gggataccag tgcaactagt atcgaaagtc aggcgggctt
     3001 gcttatgaat caatctgcgg tgtccagcgt tgtccaaaat gcttcagcca ttcgactcta
     3061 tcgctagctc actcaatcag accatgacca tcttggtcat cgtatcagtt ctattagcta
     3121 ttgtcatcct ttacaatctg accaatatca acgtagctga gagaatccgt gaactctcca
     3181 ctatcaaggt tctcggtttt cataataatg aagtcaccct ctacatttac cgtgagacga
     3241 ttgtgctgtc ccttgtggga atcgtacttg gtctggtagc tggtttctat ttacaccaat
     3301 ttttgattca aatgatttcg cctgcgacta ttctctttta tccgcaggta ggctgggaag
     3361 tctatgtaat cccagtggca acagtaagca tcattttgac cttgcttggt ttcttcgtca
     3421 attattatct gagaaaggtt gatatgttag aagccctgaa atctgtagag taaggtagtt
     3481 atttttagct gattgaactt ctatttacta atattcaaaa atcctctgtc acaaagagca
     3541 gggagctctt tgtgacagag gattttttct atagggcttt agcagctgca attgcggctt
     3601 cgaagtttgg ctcagaattg atattatcca cgtattcaac gtagcgaatc gtattgtcag
     3661 tatcgaggac aaagactgcg cgtgctaata ggtgccattc gttgatcaag agggcataat
     3721 cgcgcccgaa agaatggtca aagtagtctg aaagcataat ggcattgtca aggccttcag
     3781 caccgcacca acgtttttga gcaaaaggta ggtccattga aacagtcaat acgaccgtgt
     3841 tgtccagtcc agccaattct tcattaaaac gacgtgtttg agttgagcag atgcctgtat
     3901 cgatagaagg aacgacactc aagacttttt tcttgccatc aaaatcagcc agagattttt
     3961 tagaaagatc tgttgtagta agagaaaaat caagcgcctt gtcgccgact tgtagttgtt
     4021 tacctgtaaa gctcacagga tttccgagaa aagttaccat aggatactcc aatctttttt
     4081 cttccattgt atctgaaaca gtcagaattt tccaatgatt tgaccggaaa tgtgggcata
     4141 gaaaaaacgc cagctcacat gagaatgacg tttttcagag gcttattttg ccaatccttc
     4201 agcaatcttg tcaaggttgt atttcatcat gctgtagtag ctgtcgcctt ctttaccttg
     4261 ttctgcgata gagtcagtaa agatttgtgc gtagattggg atgtttgtgt cttgagaaac
     4321 agttttcatt ggacggtcat ccacacttga ttctacaaag agtgatggaa cttttgtttg
     4381 gcgaagtttt tcaaccaagg tcttgatttg ttcaggagtt ccttcttctt cagtattgat
     4441 ttcccagatg taagcacttg ggacaccata ggctttagag aagtatttga atgctccttc
     4501 gctggttaca atgagtttct tttcagcagg gatcttatta aatttatcct tactttcttt
     4561 atcaagtttg tctaacttat cagtatattc tttgagattt ttttcataga attctttatt
     4621 gttagggtct ttggcgctca attgtttggc gatattttta gcaaaaataa taccgttttc
     4681 aaggttaagc caagcgtgtg ggtcttcttt tcctttttca ttttgacctt caaggtagat
     4741 aacatcaacg ccgtcgctga ctgcgaagta gtctttgttt tcagttttct tggcattttc
     4801 taccaatttt gtaaaccaag cattgccacc tgtttcaagg ttgataccgt tatagaaaat
     4861 caaatcagcc tcagaagttt tcttaacgtc ttcaggaagt ggttcgtatt cgtgtgggtc
     4921 ttgcccaatc ggaacgatac tatgaaggtc aattttgtca ccagcaatat ttttagtaat
     4981 atcagcgatg attgagtttg tagcaacaac ttttagtttt tgaccagaag ttgtatcttt
     5041 ttttccgcta gcacatgcta caagaatgat tgcagaaaga aagagaacga gtaatgtacc
     5101 taattttttc attagatcct ccaaattatt agggctttgc cccttatttt aacaaatgtt
     5161 tatttttcag tttcaaatat cgttgtttgg gagcgataaa gaagctaatg agaaagaaac
     5221 tagcagctgt aagcacgata ctagaacctg ccgcaacatt aaaactatag ccaataaaga
     5281 gtcccaaaac tgaagcagta gctccgaagg ttgaggaaag gaaaatcatg cttttcagac
     5341 tattagcata cagataagca gttgcagctg gggtaatcag catggctaca atcaggatag
     5401 ttccgacact ttgcatggct gtcacagaca cgagagtcag gagtaccatg agaaggtagt
     5461 gatagaaatt gacaggcatt cccatggctt tagccaagag ttcatcaaag gaagttatca
     5521 agagttgctt gaagaaaatc cagattaaca agaggatggc tgcccccaca cccatagtaa
     5581 taaacatatc cgtatcttgg acggccagga tattaccaaa aaggatatgg aaaaggtcag
     5641 ttgaactttt agcgacacca atcaagatga taccgagggc taagaaagaa gaaaaggtaa
     5701 tgccgatggc ggtatcgctt ttgataatcg agtttccttt gatgtaggta atgatgatgg
     5761 cagctagcaa tccaaagaca atggctccga taaagaagtc aaggcccaag atgaaggaga
     5821 gggctacacc tggtaagaca gcatgtgaaa tggcatctcc catgagtgac atcccgcgta
     5881 gaataatgaa acatcccaca gctccagcta caatcccgac gacaatagct gttatcaagg
     5941 cattttgtag gaaatggaat ttttgcaatc catcgataaa ttctgcaatc ataggtcacc
     6001 tccattgaaa aagagttgat taccgtaagc ttcttttaga ttggtttcgg taaaagtttc
     6061 ttttgttgga ccaaaggcaa tcacttctcg attgacaagt aagacttgat cgaagtagtg
     6121 gggaatcttg ctgaggtcgt ggtgaacgat gagaaccgtc ttcccagctt ttttcaaatc
     6181 tctcagcgta ttcatgatga tttcctcact gacagagtca atcccagcaa agggttcatc
     6241 caagaggata tagtcggctt cctgcaccaa acatctggca atcaaaaccc gctggaattg
     6301 acctccagac agttgactaa tttgacgttc agcgtagtca gctaggccga cgatttcaag
     6361 ggcctcttgc actttcttcc aatgtttagc ctttaaactt cgaaagagag gaatagaggg
     6421 aaatagtcct aacgagacgc attccttgac cttgatggga aagttgtagt cgatattgat
     6481 tttttgttcg acataggcaa ttcggtgtaa ggatttttta acttccttgt catcgagaaa
     6541 tgcctgacct tgatgtggga taattcccag catacctttt aatagtgttg atttcccagc
     6601 gccgtttgga ccaatgatgc cggtaattgt tggtccatgg agcactagtg aaatatcctt
     6661 aagtgccaac gtttctttgt aggagacact gaggttttcg atacgtatca taaacttgta
     6721 ttcctcctgt ctcttaatat acattaaaaa aaaaattaag tcaagttaat ttttgaaaaa
     6781 attaaaataa taactgaaaa atagat
//
LOCUS       DAVBHS010000107         6795 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVBHS010000107 DAVBHS010000000
VERSION     DAVBHS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.107"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..207)
                     /locus_tag="VQ651_001566"
     CDS             complement(<1..207)
                     /locus_tag="VQ651_001566"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754539.1"
                     /translation="MENMTNPLLLVQIRVHGYLLDFASSEGQRQKHYRLKNLPQTVEL
                     TVDDVEEDVDLTLPENRSYQEADFF"
     gene            complement(275..916)
                     /locus_tag="VQ651_001567"
     CDS             complement(275..916)
                     /locus_tag="VQ651_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754540.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(918..2948)
                     /locus_tag="VQ651_001568"
     CDS             complement(918..2948)
                     /locus_tag="VQ651_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2754541.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEVLSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQFICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSTYYQRLFTDYL
                     QNFSSESVEVTSQKYYLPQVSLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(3217..4116)
                     /locus_tag="VQ651_001569"
     CDS             complement(3217..4116)
                     /locus_tag="VQ651_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2754542.1"
                     /translation="MLIGQKIKEIRIEKGVSRPYFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(4188..4748)
                     /gene="rnmV"
                     /locus_tag="VQ651_001570"
     CDS             complement(4188..4748)
                     /gene="rnmV"
                     /locus_tag="VQ651_001570"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HET2754543.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVRHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(4748..5521)
                     /locus_tag="VQ651_001571"
     CDS             complement(4748..5521)
                     /locus_tag="VQ651_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET2754544.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     RALELVDEYDQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKVTDLQEAAKELPLDKILVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(5826..6719)
                     /locus_tag="VQ651_001572"
     CDS             complement(5826..6719)
                     /locus_tag="VQ651_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HET2754545.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANAGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIVMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
BASE COUNT         1909 a         1619 c         1171 g         2096 t
ORIGIN      
        1 aaaaaaatca gcttcttgat aacttcgatt ttcgggtagg gtcaaatcca catcctcctc
       61 cacatcatcc actgtcagtt caaccgtttg aggtaaattt ttcaaacgat aatgcttttg
      121 cctttgccct tcactactag caaaatcaag caaatagcca tgcactcgga tttgaaccaa
      181 aaggagagga ttggtcatat tttccatgat gaggatatct tataaaccac ctctggcact
      241 aggataatca gataccctgc gaatacggat tttttcacac atgagcaagt ttcctcatat
      301 caatgatttc atcagcctta ttccagacta ggggattatg ggtcgcaatg atgataattc
      361 gattttcatc tttcaaatcc accaagagat tcataacctc ctctgaattt tcaggatcaa
      421 gagctgctgt tggttcatct gccaaaatca agggtggatt tttcaaaata gttttcgcaa
      481 gggcaactcg ttgggcctct cccccagata aagtataaat tttttgttct aaatccaaat
      541 accctagatt aactttttct aaagccccca cttgcctttc caaacgttct acttttgaga
      601 ttttctgacc aacaaaaccc aaatccaaat tttctttgat tgattggttt tctaagaggc
      661 caaaattttg aaagagatag cccatctgat ctcgaaaata ctcacgagtg ggaatggttt
      721 ttaaatcttt cccctgatag agaacccttc ctccatctat cttttctagc tttcccaaga
      781 tattcagcag cgtcgtcttt ccgcttccac tctttccgat taaggcataa accttgccct
      841 tctccagctt gagattcaaa ccagagaaaa tcgcgcggtc attaaatttc ttttccaatc
      901 cttgaatatc aatcatctta ttttcctttc aatactgcca tgctaccagc ttcttctttt
      961 ttgtcctgcc ttactaaaat caagagggcg ttaagcgtaa acaaagctac aactagagcg
     1021 ctaatcaaac catctcttgt caaaatacta gataggacta ggccaagcaa gagaactcct
     1081 ccttgcgcca gtaaatactt gccatgaagc tcatagattg tcataccagc aagacgttta
     1141 atcataagct cccgtctgaa ctgctcaaaa tagagaagat tcatggaatc aaataacaag
     1201 atagccgtag acagggtcaa aatcgtccca ataaggagag agtaaaactc tacccgtttg
     1261 tcatttagta ccttggcaaa aaatagctgg ctttttacca agtaagaaac cttgtgatac
     1321 agaccttgct cttttagagc tgtgatgctg tctccatatc gatctagttt caaggcacta
     1381 ttagccgtag ttccccacaa catccctgca acaggtcttg ttccagtcgc ttgcggcgtt
     1441 agaactacaa taatcggatc ttttaggtac tggcgtgttg tcttgtaccc atcattatag
     1501 aggaaacgtt cctgtcctgt ttctgtaaaa gctagactta cctgtgggag gtagtatttc
     1561 tgactcgtca cttctacact ttcagatgaa aagttttgca ggtaatctgt aaacaatctt
     1621 tggtagtagg tagactgctc tcgcaagctc tcaggcaaga ttagcccaaa ctctccctca
     1681 gacaagtttt gcatcttgtc cataaactct gaagaaacgg taatcttttc ttctatcaga
     1741 tagcgtggtg agacatagat aacattccct gacggtgtgt agtcactgag acgattgcca
     1801 tctaggtcca cttctgctcc gtctgagaaa ttgtcaacat tgctcataat ataaaaagag
     1861 tctgtattgg ctaaccgttc ttcagtaaat gtctgccact cacgattatc cttacgcgtt
     1921 ccttcttcat cggcaaatgc actagaccaa ccaaaggata gacggtaacg gtctgaggac
     1981 tggctccatt tattgctagc tctctccatt tcctgcattt cacggtagtg gggtaggaga
     2041 gctgtcgcac tcgatccgac caccaataca gctaagagtt gccccaccat catcaatgtc
     2101 atcatacgtt tgagagggag tttccctttc aatagatcca ccagactatt ttcctgtaaa
     2161 ccaagtagat agacgacact tagtaaggta gaaatccctg ccagggtcaa tccataaagt
     2221 agaagagcaa tgatgaccag ttgcaccgtt gccataaaca aggcaccttg ataccagaga
     2281 atccccaatc ccaaaagact ggataccagc actgagcaga taaactgtct cacatcttct
     2341 aacactggtc tgagagcaac tccaaacaag ctctcaccag ctattaagcg aatccctgcc
     2401 tgacgaaggg atttgatccg ataaatcagg gtcagactca taaaggtcag cagaaaaata
     2461 gctaaactca gtagcaccat aggggtggcc gccaatagta agactatcga aaatggatct
     2521 tctccattcg aaacaaagcc ttgatagcca agttctttca aggtggtctg taagctcact
     2581 ccatccaaac ttcctgacac aatcaagtaa gaccctaata aatcactcgt ttctgcactc
     2641 tccttggagg aaatggtcag accttctgga agctctccct caccataagt ggcataggta
     2701 aagagtgttt ctccagcttc atttggctca acgattcgac gggcgatgag actattatgc
     2761 tctaccgcca agtgactcaa aacctctgtc acctcttcgc gactagctac tggttgattt
     2821 atcatcattc ctgtcacatc aacagaagga tagttaccaa ataaaatctc ttctcgatgg
     2881 tcaacggaag ttatcaccat ataaagcgat accaatacca ttgaaatcaa aataaataaa
     2941 cgtttcatca tttcctccaa aaagttactt ggtcacttag tgagaaggtg accacagtca
     3001 ctgcgatgtt aagaagctta ttaagttctt tctactagca acatcttcaa atacattata
     3061 tccgtttttt caagaactat caaggacatc aatgtcctta aaatctaact atttcatatt
     3121 atttttagct tatatctaaa aaaacagttc taaagacgaa ctttagaact gttttgatac
     3181 gaggacaagg acatttttgt cactgattca ctatgtttat aaatattttt tgacatcctc
     3241 ttgccattcc tctgtcaatt tctttactag aaatccatct ccaattagct tcgcaaatgt
     3301 tttagaagac tgataaaatg attcagctaa actaaaatct ctttcttctc ttaatgcttg
     3361 cttccacaaa aacataaata caagaggttt cttttgataa tcttgagttt tatccataat
     3421 ttcttgtaaa cacgatagat aatcattaaa acaatcagtt actcctacct tttcaagaca
     3481 acacattcct gaaataagag tatctctaat tataaagaag ttttctaatt tagttacctt
     3541 tttctgttcc aaaagaaaga gcattagctt ttcgatttga tttataaatt tggcaagatc
     3601 ttttctaatg agcatttgat aaaaaaacaa acgaatcaac aacatatcat ttgttgaata
     3661 aacttctttt tctatgatct gagacatatg ttcctcaagt attaactctg catattcagg
     3721 ccttctagtt ctagaagtat ccaaacacgc ctgcaatact tcacatgcta attgttcttc
     3781 tttaggaaga ttatcaaagt atgttttata aatctcttcc aaacacgctt ccttcttatc
     3841 gtactcctct tctttaccat agattggttc acgtaaaatc tggtatttca attctaaata
     3901 agcaggagga agagctgaaa aatccggcat aaggctataa actggaactc ctagccggcg
     3961 agcaatatag gctaacttgg gcaaactcgg ttgcgaagct ccactttcaa ttcgcgacag
     4021 ttgacgaact gtcagttctt gctcatctcc acaaaaatat ggacgactaa ctcctttttc
     4081 tatccgaatc tctttaattt tttgaccaat taacatcaga cgcctcctct tctacctgct
     4141 tcgaaaaata ctgtgaaact taaaaaattg taacggctac atctttccta ctcataagat
     4201 ttcatagctt cttccacttc tgccaaagta accccaaaca actctaggcg tttgaggagt
     4261 tgcttgccgt tagaatagcc gattcggaga gtctctccga gatattctct acgcttacgg
     4321 ctgtctgccc ctgctagaaa accaaggcga atcaagtcgc tacgactaat gtcaaactga
     4381 ctctcatgtt caaattgttc tgtcacctga gctagagccg ttttcaggtc ttcatagctg
     4441 gcatgctcaa ttcccagaga acgccccttg gtcttggact ttggaacagc ttcatctcgc
     4501 ttgagaaagg catgccgaac tgttggaatg gccgtcataa tcatgcgccg aatccgctcc
     4561 ccattaaaat ctgggtctgt aaagacaatg actccatgac gttggtgcag gcgctgaatc
     4621 cgctctatat cctgagcatt gatggcagaa cctcgagtct cataggtctc cacatcgaaa
     4681 taacgtttga gattgaccgt atcatcacgc ccttcaacca cgataacttg agaaattttc
     4741 tctttcatta cttgctgtcc aatccaaaaa tgcgttctgc atttgcagtc gttgctaccg
     4801 ccagctcttc tgtcgtcata ccacgcaagt cagcgataaa gtcgaccaca tagcgagtat
     4861 aggctgtttt attttcacga ccacgcttgg gtacaggtgc taagtaaggc gcatctgttt
     4921 ccaccaaaat cttgtccaaa ggtaactctt tagctgcttc ttggaggtca gttaccttct
     4981 taaaagtcac cactcctgag aaggaaatgg tcataccaag atccacaaac ttctctgccc
     5041 actcaagcgt ccctgaaaat gaatgcatga taccaccacg aggaccaacg ccctcactct
     5101 tgataatctc ataggtatct tccagcgcat cacgggtatg gacaacaaaa ggcaaatcca
     5161 agtccttaga tagctgaatc tgacggcgaa aaacctgctc ctgcacctct ttgggcgctg
     5221 tcatccaatg gtaatctaag ccaatttcac ctaaagccac aaccttggga tgttttaact
     5281 tatccaacaa gtaagcctca acttcctctg tataagtacc agcttctgta ggatgccaac
     5341 caatagtcgc atagagctga tcatactcat ctaccaactc caaggcacgc tcaatcgtcg
     5401 gtttatcaaa accaacaata ttcatctgtg tcacacccat ctcagcagcc aaggcaattt
     5461 cttctgcctc acgacctgca aattcttcta cattcaagtg tgtatgtgta tcaaaaatca
     5521 tctcttctaa cctcgttttc tatcttctat tataccaaaa aagaggaggg gcacctaatt
     5581 tttcggtttc ccctcctctc ttcaatagag agctattctg ctatcttttc tatccgatat
     5641 tgcccatctc ctattccaca gttagagaca gaagagattg gctacattga tagaatattc
     5701 ctattcagct aaagtatgtg attatcttgg gttctcactt ttctcaccca tcactaggat
     5761 tgatgacttc tcaaaatccc ccttataatc taactttaag tagagaatag aattttctag
     5821 ctgttctatt ttctacgtct agtctttctt cctagcaaag caaaaccact tgttgccacc
     5881 actagaccaa gactagcaag tgctgcactt gatgctgaac ctgtgttagg taattgtttg
     5941 ccagcaggtg actggtaggt aacatcttgc attgttcctg cagttgcttg cggtgcactt
     6001 gttgtcttag gagcagcatt aggagccact ggtctcttag gaactgtaac catgacaatg
     6061 gcttgcactc caatcgtttc tgcttccttc acatcttcca tagttttcgc attttcaatc
     6121 tctttgagag ctgcttgttt ttctgcttcc actcttgcta aaagttctgc tttttcttta
     6181 tcagaaagcg gtgcgccttt gatttcatcc tgcttgtcct tggctgcttt ttcaatggca
     6241 tttttagctg attctttttc agtagctaat tgcttagaag cttgtgattg agctgagttt
     6301 tgtgaacctg tgtgagctga tccacccgcc tggctaccac cgcctgcatt cgcattagca
     6361 tctgagcttc cagtattgcc tcccttatct ccacctggtg tattttctag cttatactct
     6421 ggtgtagctt cttgagttac agattcacct ttcgcagatt gttgaactgt atcttttcct
     6481 agaatcgtca ctactgaacc atctgggaat gtgattgttg ctgtaccatc tccagctaca
     6541 ttgattgtcg ctccgtctaa tgctgaacca tttgcttgta aaatagcaac cttaactttt
     6601 tcttcatctg ttaaatgagt tatatctttt acatctactc tttgagcagg tgctttaaca
     6661 gttgattttt cagcagtcgg tttagtgcta tcttgtgctt ttggatcttg tgctaccaat
     6721 tgatttcctg gaattgtaat agctgaacca tctttaaaga cgattgtcgc tgcgccatct
     6781 ccagctactt caact
//
LOCUS       DAVBHS010000108         6749 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVBHS010000108 DAVBHS010000000
VERSION     DAVBHS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.108"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(116..1540)
                     /locus_tag="VQ651_001573"
     CDS             complement(116..1540)
                     /locus_tag="VQ651_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HET2754546.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMLKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2084..4363)
                     /gene="pulA"
                     /locus_tag="VQ651_001574"
     CDS             complement(2084..4363)
                     /gene="pulA"
                     /locus_tag="VQ651_001574"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HET2754547.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4475..6433)
                     /gene="ligA"
                     /locus_tag="VQ651_001575"
     CDS             complement(4475..6433)
                     /gene="ligA"
                     /locus_tag="VQ651_001575"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET2754548.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6526..>6749)
                     /locus_tag="VQ651_001576"
     CDS             complement(6526..>6749)
                     /locus_tag="VQ651_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2754549.1"
                     /translation="FFTQVQTKVESEFLGRVLSTIFTLAILFMPIAKGFMTVLPSVHL
                     SSFLIIGSGVIILSCISFIYVRTHFEKLI"
BASE COUNT         1983 a         1491 c         1227 g         2048 t
ORIGIN      
        1 tgtactattc tagtttcaat atactataat accaaaaagt tcgtattttt tctataatta
       61 ttattatttt atagcaaaaa ataaaaccag gaaaacaatt tcctgatttt acacgttatt
      121 tcacatcaaa aacaatggat ttgacatttg tcatcgcttc aatgctatat ttaattcctt
      181 gcactccagc tccagaacct ttgacaccaa ggaatgggaa attatctgga ccacgctggg
      241 ttttattatt aatgtggact gtacctactt caagtttttc agcaatttca aaggcttttt
      301 tgaaatcatt tgtaaagact gatgattgaa ggccgaattc agattcgttg gcaaaggcaa
      361 tagcttcctc tacactagcc acacgaatga ttggtaaaac aggaccaaat ggctcttccc
      421 atgccacttt catatctttt gtaacttggt caaaaagcac tggccagagg agattgccct
      481 cacgtttgat tggtgtaaga gcctgagcct ctttttcttg tgcatcctca atcaagcccc
      541 aaatgaagtc ggctgaagca ttgtcaataa caggtgtaat atcagcattg tcaaatggat
      601 caccaactgt taatttagaa acttcttcct gaagcaaagt agctaattta tctgctacac
      661 tttcgagaac aatgacacgt ttaatggccg tgcaacgttg tcctgagtag ctaaaggctc
      721 ccgcaacaat ttgcttggca gcatgttcca aatctgcatc ttctagtaca agagctgcat
      781 ctttcccacc aagttccaac atgataggac gcataccagc taaacgacca atacgttctc
      841 caataggagt tgaacctgta aagttgatga agttgacttc tttgtgctca atgatataat
      901 ccccaatttc tgaaccacga cctgtaatgg tgttgaaaac acctgccgga atccctgctt
      961 cttcaaatgc tttagccaac aagagtccag aaatggaacc ttgtgttggt ggcttcaaca
     1021 tgaccacatt ccctgcaatc aaggcaggtg caattttaga agcagataaa ttaactggat
     1081 aattaaaggg agcaatcgct agcacgatac caactggttc acgacggaca acagccagtt
     1141 tgtttttact tgctgcctca aaaccaccac cttccattgc ttgtccagtg atacggagac
     1201 cttcctcagc agcataacga atcaagtctg ctgtacgcac tacttctcca attgctgctt
     1261 taatcccttt tgctacttct ttggcaagga tagtaccaat tttttcctta tcgcgttcta
     1321 aaatagctgc tgttttatgc aaataagccg cacgttcaat tgctgataaa gctcgccatg
     1381 ctggcagggc tgcacgcgca gcttgcatag cctcatcagc ttcagtctga gtcatggctg
     1441 gaactgtacc caattcttct tgattgattg gtgaataaat cgtaatttct tgttcagatg
     1501 atttccattt tccatttact aaattctgat atcttgtcaa attcttgtcc tcctgaaaat
     1561 gattaagata tattttacca aattttcaga aaataacaac cctttttgca aaatttttga
     1621 gattattttc acaagcttga tttttcaaag tatactcaat aaaaataaaa aaatccacta
     1681 cgtcaaggcg aggctaatgt ggtttgaaga aattttcgaa gagcgtgaat gagtatcatc
     1741 tatagtaaaa taaaaaaact gaacaatttg gttggggaca gccaaaccaa tttctcacaa
     1801 tgtttcagaa acaagggtgt gctattccaa tttcagccta ctataactgt catagattgc
     1861 tgaaacaaag tctaggtaaa agtcttcata ataaaaagac ctcctatcaa atgttcaaaa
     1921 actttgatag gaggtcttgt ttcgtgaaaa tatttatcaa attttctata caagtgagct
     1981 gttagccagg ttctttctat tctttcaatt tcaatgaatg gattttttac caatactcat
     2041 aactgggaat ttttctgtct aaaaatagcg agatagatgg tatttataaa acactcaaga
     2101 cagctagact aatatcattt aaaacattat cttcttttga gcgactgttg gttaccaaca
     2161 tagctaaatt tcctgcattt tcaaattgat agggttctga tttagcattc acaactacca
     2221 agaggtgttc tttgccgtga acttcataga taaggcagcc gctatgttca atcgcagaat
     2281 gcacaaagac atgatggtaa atttcatcat agctagagta agaaaaggca ccagtttttg
     2341 tcttcaatcg gatgacttga cggataaact caatactgtc ttgacgctca ttaatcaagt
     2401 tccagttcac ttggttcaca ctgtcaggag cattatagct attcatcgca cgctctctat
     2461 catcatgggt caactcacca ttttcaccag tcgcaaccag tttggtacga ccaaattctt
     2521 gaccgatttc cataaaggcc atcccctgca tgagcagatt catggctgtg gcagtttcga
     2581 ccttgcgcat gatttgctct gaactttggt ctggatgaag ggttgccaat aaatcgtgaa
     2641 gattgtaatt gtcatgggct tctacatagt taagcacctg atttggatgt gtatagcttc
     2701 ctaattcacg acttcctagg attgctttag ctagaattgg ctctgtcgca gcaccactga
     2761 caaaacctga cttgatagca ccataaactt ctcccccttt gacagcatcg cgctgattgt
     2821 cattaaagaa accaatattt ggcatctggt aggcattgtc cttcttggcc ttatcataag
     2881 gggcaagacc tgttcccata tcccatcctt ctccatagag gataatgttg gagtcaattt
     2941 catccaagct ttgacgaatc atctgcatgg tcttgacatc atgaatcccc atcaagtcaa
     3001 aacggaagcc gtcaatatta tattcctgca cccagtatag aagagaatca atcatatact
     3061 tgcgaaacat ttcgtgttca ctggctgttt catttccaac acccgttcca ttctggaagg
     3121 taccatctgg attcatacga taatagtaat cagggactgt tgtttggaat ggtgcatcaa
     3181 caactgagaa ggtatggtta tagactacat ccataatgac tccaataccc gcatcgtgat
     3241 aagcttgaac catcaccttc aaatcacgaa tgacctgagc tggatcatct ggattagttg
     3301 aaaaactagt ttctggcgcg ttatagtttt gtggatcata accccagttg taggttacat
     3361 ttccatcctc atcgtattct ttatgacggt ctgcaattgg ttgcaattga acataattgt
     3421 agcccagctt cttgatgtaa tcaaaagcag ttgactggcc gtattggtta actgttccag
     3481 cctgagcagc acccaagaaa gttcctcgaa gatgttcatc tacacccgat gtaggtgatt
     3541 tagtcaaatc acgaatatgc atttcacaga taactgcctt acatggattt tccaagcgcc
     3601 aagtagcctc cgaaccgtgc ttaacctcga agttttcaac ttgcttttct acatggctca
     3661 gaatagctga acgtttgcca tcagggctgg tcgcgattgt ataaggatca cgtgtcagtg
     3721 tttggtgatg agggaattgg acttgatact gataagtctt acctaccaaa tcttcttcaa
     3781 catccaaact ccagacaccg attgtattgt ccttatgatt ataagagtag ctattgcctc
     3841 ttttcatctc aaaagtcttc caaacgggtg catcattagc agctgattca taaacgacaa
     3901 cttgcacttc tgtcgctgta ggtgaccaga gggaaaaatg agcctgattg tcctctacac
     3961 ggcaacccaa ttctccttgg taaccccaat gatgatcaaa actagcactg ttaatggcct
     4021 tatcaaaggc aaaaggattt tgatttttat agaaaggact ggcaatagca ggattttcag
     4081 agtaataaat cctatcatcg ccttccaaaa tccagacctc tgttaatagg ggatagtgat
     4141 taaaacggat agaatattct ttactagttt gacctgtatg aaccacaaaa ttcaagcttt
     4201 ctataacatg tgaacttggg tgttcaaagc taaataaagc tccaaaataa tcttctttgt
     4261 aggttagcaa atcaattcgt tgatcctgac tttttacaaa ggagcaagtg tcatattctc
     4321 cattcttacg atggtaatga atgcgcatag ggtagttata cattttttat ttttcctttt
     4381 tactttgttt ctatttcact aataaatttt tgtcaatctc gtctcaatta acagacatag
     4441 tcatattctc taaactctgt ttttaaacga tccattacaa actttctagc catgcctcat
     4501 ctctgacctg gataccaagt tcttgtgctt tttgcagttt acttccagcg tctgcaccta
     4561 ccacgacgag gtcggtcttt ttagaaacac tacctgtcac tttggcaccc agactttcga
     4621 gtttactttt agcttctgag cgcttgagtc gttccaattt tcctgtcaat accacggtca
     4681 aacctgacaa ggccgcatcc gctactaccg tctgtccttt atagtccaga ttgaccccag
     4741 tttctttcaa ttctctgagc agaatttcag agccttctgt cgcaaaataa gtctgaagac
     4801 ttttggcaat cacgccacct agactttcaa tactagccac ttcctctgaa tctgcctgag
     4861 acagattttc aattgaatgg aaatattgaa gtaaaagctg actagccttg cttccgacat
     4921 gacgaattcc caaaccaaat aagagcttct cggcagaatt ttcctttgat gcttggatag
     4981 cctgatacag tttagcagcg gacttttcct taaccccctc taaaaggagg aaatcctctt
     5041 cttgcaaacg ataaatatcc gccacatcct tgactaaatt agcagcaaaa agcttctcaa
     5101 caatagatgg accaaggcct gtaatattca tagcatcacg agaagcaaag tgaatcaagc
     5161 cttccatgat ttgagcaggg caacgcggat tgatacaacg tagggccact tcatcttcaa
     5221 agtgcaacaa gtcagagtta caacttggac agtttgtagg gatatctagt ttttcttcag
     5281 aaacccgttt ggactctacc acacgtaaaa cggcagggat gatgtcacca gccttatata
     5341 caatgaccgt atcgtctttt cggatatctt tttcagcaat ataatctaca ttgtgcaggg
     5401 tcgcacggct aacagtcgta ccggcaagtt gtactggtgt tagattagca gttggagtta
     5461 caacaccggt acggccaact gtccagtcaa ctgataagag ttgagcttct ttttcttcag
     5521 cagggaactt gtaggctact gcccactttg gagccttaac ggtaaaacca agttcttctt
     5581 gacttgctag gtcgttgacc ttgattacca ctccatcaat atcgtaaggc agattttccc
     5641 gttcctgtcc tacttcttgg ataaaattcc agatttcatc tatgttttca gccaagattc
     5701 gcttaggatt gaccacaaaa cctagttgtt ctaggtactt caaacccttt tcttggctat
     5761 cacgagttga agggctggct tcttgataga gaaacgttgc aagattacgc ttggcaacta
     5821 ctgctgtatc caactgacgc agagttcctg ctgccgcatt acgaggatta gcaaattcag
     5881 gctctccatt ttcttggcgc acttggttaa cttggtcaaa ggaagcgcgt ggcatgtaac
     5941 attccccacg aactgtgata tctagttctt ctggcaaagt caaagggatg tccttaacac
     6001 gcttgaggtt ttctgtgata ttttcaccaa ttgaaccatc tccacgtgtt accccagcaa
     6061 ccaaaatccc cttttcataa gtcagcgaga tagataagcc atcgattttc agctcacaaa
     6121 tataggtcgg atgagccact tccttacgaa cacgcgcatc aaaagcatct agctcctcac
     6181 gtgaaaaagc atcctgcaaa ctataaagag gatactgatg actgtatttt tcaaaaccat
     6241 ctaaaacctt gccaccaaca cgatgagtcg gactgtctgc tagcacttgc tctggataag
     6301 cagtttctaa ctcgaccaac tcacggtaaa ggcggtcata ctcactgtct gaaaccgagg
     6361 gattatcgct ggtatagtac tcagtcgcat agcgattgag caaagcgact aactcattca
     6421 ttcttttatt cataagacca ttttaccata aaacaagccc tcctcacaaa cgagaagggc
     6481 ggaaaaaaca cttagtttga aattattttt gaaactcaag caaccttata tcaatttttc
     6541 aaaatgagtt cgaacataaa taaacgatat acaagacaag atgataacac cacttccaat
     6601 tatcaggaaa gaagagagat gtacacttgg caagactgtc ataaatcctt ttgcaatagg
     6661 cataaataga atagctaagg taaaaattgt actcagtact cttccaagaa attcgctctc
     6721 aaccttggtt tgtacttgag taaaaaagt
//
LOCUS       DAVBHS010000109         6712 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVBHS010000109 DAVBHS010000000
VERSION     DAVBHS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.109"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(124..1266)
                     /locus_tag="VQ651_001577"
     CDS             complement(124..1266)
                     /locus_tag="VQ651_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET2754550.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQGTTPAEKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            complement(1432..2052)
                     /locus_tag="VQ651_001578"
     CDS             complement(1432..2052)
                     /locus_tag="VQ651_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2754551.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQNLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNTFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            complement(2056..3336)
                     /locus_tag="VQ651_001579"
     CDS             complement(2056..3336)
                     /locus_tag="VQ651_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET2754552.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKLTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            complement(3397..4881)
                     /locus_tag="VQ651_001580"
     CDS             complement(3397..4881)
                     /locus_tag="VQ651_001580"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET2754553.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTAELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVYESDS
                     YIEDPHTAVASAVYRKYQAVTGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            complement(4957..6234)
                     /locus_tag="VQ651_001581"
     CDS             complement(4957..6234)
                     /locus_tag="VQ651_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HET2754554.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            complement(6341..6424)
                     /locus_tag="VQ651_001582"
     tRNA            complement(6341..6424)
                     /locus_tag="VQ651_001582"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6388..6390),aa:Leu,seq:caa)
     gene            complement(6434..6505)
                     /locus_tag="VQ651_001583"
     tRNA            complement(6434..6505)
                     /locus_tag="VQ651_001583"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6471..6473),aa:Gln,seq:ttg)
     gene            complement(6519..6591)
                     /locus_tag="VQ651_001584"
     tRNA            complement(6519..6591)
                     /locus_tag="VQ651_001584"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6556..6558),aa:His,seq:gtg)
     gene            complement(6606..6676)
                     /locus_tag="VQ651_001585"
     tRNA            complement(6606..6676)
                     /locus_tag="VQ651_001585"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6642..6644),aa:Trp,seq:cca)
BASE COUNT         2028 a         1579 c         1191 g         1914 t
ORIGIN      
        1 cagataaaac tgacgtggtt tgaagagatt ttcgaagagt atgacaaaga aaaaacgaac
       61 gagaatcgct tcccatcacg aaagctagat ctcattcgtc aaaatgatgt taccaagtcg
      121 ggtttatcca ttcattgaaa cgtgaacgtg atcatagtga ttttctgtca cactaccacg
      181 gtctggcatt gggttccaag tgttagctgg cccatatttg ctatcgaatg gagcatagaa
      241 acgttgtttc cagatgatgt aactaatgcc acggctggcc atgttttgaa tagcatattc
      301 cgcaatctta tcccctaatt ctgaacgttc tggtaccata aagtcgatag ccaaaccttt
      361 tccgtgatct ccactgtctc ctggacgata accgctaaag gatgtaatgc caaacaagtt
      421 agcaatttct tctttaaagg cagctgtttg tggttgaaga cctgcatttt cagattttgc
      481 tactgcaagt ccagcataat cgggcgcagc tggagcctcg taagttgttg aaattatttt
      541 cgtctcttct ggttgataag tagaaactgc tgctgtagct ccatttgatg atgctacttc
      601 ttttgcttct gaacttgttg tagttgcttc cactgctgat gcagcttgag ggcttgcttg
      661 tgtttcctgc ttctcagctg gagtcgttcc ctgaggagct tcttctgcaa ctgcactgct
      721 tgtttcggcc gtttgctcct ctgggacaga agtgcccgta gatggtgcca cttcttcaga
      781 agcaatcact gtctttgtaa cttctgaact tgatgctact tcttttggag cttctgcaat
      841 tggttgagaa aggtctgcaa cctgaacagt ttgatcatca acggtcactt gattagtggt
      901 caaatctgct gtcgcagttg tcacttcttc actagagtct gcttgaggtg tttggatttc
      961 aacttctgtt acttcttctg cttcattgac agtcgttgtc aaaacagttt ctgggaaaat
     1021 caagtccata ttagtgattt tgttcagatt cgcaagcact gtgacatcta cacccaaggc
     1081 ttctgcaatg gtgctcaaag tatcaccata ctgtacggta taacttgttt tgttgtccgt
     1141 tttagtcaaa tcgttttgga tttgctcaac actacgtgca gtccaaagaa cttcttctgc
     1201 ttgagttgcc aatactgggg caaatgacaa ggctactgtt gacgctaata acattctttt
     1261 cttcattctt tcaaattcct ttcaaatgag tacctgtcta tcataacaca aaaaatgtag
     1321 aaaaaaagcc ctctcgggcc tatttaacag aactgtctat tccttgtaac aaactagatt
     1381 acttgaaata gtttgttagg tctctgtcac aaaactgaca caatcttttt atcacttagt
     1441 ctcaaaaata cgggaaatat ctgctaacgc ttgaatcctg tgattccctt cataagtaga
     1501 ctctaaaaag ttgatacttt gaatcccact attctgggca aattccacat ccagagtccg
     1561 atcccctata taataagtat tatcagaatt caactgatac ttatctagca gataggtagc
     1621 cgcttctgga cttggcttcc gcacaaagcc actctgactg gtcaaaatct ctgtaaaata
     1681 ggattccacc cccaagtcct tgagaatggt aaaagtgttg ttccccttat gagtatatat
     1741 aaactgctga attcctgatt cgtctgccca agctagcacc tcacgcgcac ctggcatcaa
     1801 aactacctga gcattcttct cagccagact ctgggcacgc acctgattta gcacctcaac
     1861 atccagattt ctatcttctg ccacccgcac aagcaaattt tgcaccgaat acttgaagat
     1921 aaactctctc accttctcct tatcataagg aatagaaaac tgagcaaaag tctcctcaat
     1981 ccctgataaa atcgcttcgt aagagtccaa taaagtcccg tctaaatccc aaataaaagc
     2041 tgttttttgc atttcctatc ctttcaagct catatagcgc tggtaacgat agcgtagaaa
     2101 taaccagcga aaaccattat ccaagagaga ccctgcccaa ataccaggca agccccaacc
     2161 aagcacaata cccatcagat atcctgtccc aatgcggata caccacatgc ctatacttgt
     2221 cgcataaaaa gggaggcgtg catttcctaa tccctgccag actgccgtat agatgactgt
     2281 tcctgtcgtc ataggggtac caagtagtga aaacagtgtc actagaacac tagcctccac
     2341 cgctagagaa tcagtcgtat agagatgagt taatggtaca cccaagacat atatactaaa
     2401 ggacaggggc aacatgagga acagagaaag ccaaaaggtt tgtttactca aactagcaac
     2461 tcttttccaa tcatcctctc caactgctcg ggccaacagc atgaccgttg ccgtagcgac
     2521 accaaaggca ggcatatagt taaactgggt caagacttct ccgattgcat tcccagcaac
     2581 tgcctccgtc ccaaaagaaa cgaccaaggc aatgatcact acatctccag ccctcatcat
     2641 aagtcgctct ccagctgctg gtaaagccaa ggtcaacagt tccttatcta aaccaaaagt
     2701 tagcttccca taaggcagtt ttaattgtga ccacaaaatc acaagaccaa ccaaacgaga
     2761 cagaattgtc ccccaagcaa caccagctat ccccatatcc agaacaaaaa tagctagact
     2821 tgaaaaaaga atattcaagg cattggataa aaaactaaca tagagaggca gacgtggatt
     2881 atgcgttgca cgaatcaagg ctcccaaact agtcattaaa cctaagagaa caatcgatcc
     2941 gcctaccaaa gatagataca gtccaccact ctcagctaca tccctctccg tccccaaaag
     3001 tcctatcatc tctttcccag cgaagatgga caaaaatcct aaaaggaaac ttaatagtaa
     3061 ggtaatcttc aacgcctcag tcacatgata ggccaacttc gactggtctt tctgccctat
     3121 gctttttgaa ataacactgg aaatagcagc tcccagagcg atgaaaatcg cctgataaat
     3181 ggtgataata ttaccagcta ctgaaacccc tgaaatagct atcaatccta aatgagcaac
     3241 caaataactg tccaccattc ccattagcat ctgcaaaaag ttttcaccca tagctggcaa
     3301 tgcaatatta agaatgtctt tatttttctt aaacaatctc tcctcctgat gaaaagaaac
     3361 tcagttggtt tcccaaccga gtttgctccc tctgtcttaa agtcctaaat aagcctcaac
     3421 cgctgcttgc atgtcagcag ctgccactgt tgtcttgtga cgaattggag ctatttcaag
     3481 cccatcaact gctggtggca ctgcaacgcc tgagatttca tgtaattgag ccaaggcttc
     3541 aaagtctgtc aaacctgctt ttccagttac agcttctact gcaactactg ggaacttgta
     3601 tggactagct gttgaagcaa tcactgtctt agttacatct ccagtaaccg cttggtattt
     3661 tctataaact gctgaggcaa ccgccgtatg tggatcctca atataagaat ctgactcata
     3721 aacacgcttg atttctgccg ctgtttcttc ctcagtcgca tattcagctg caaagaggtc
     3781 caaaatctct gcatcaaagt ctgtcaactt atattgtcct tgcgtgttca aggcattcat
     3841 aagttcagct gtcttttcag cattatttcc caaaagatgg aaaatcaagc gctccaagtt
     3901 tgaagatacc aagatatcca tagatgggct ggttgttacc ttaaactcac gttttttgtc
     3961 atagacacgt gttttaaaga agtctgtcaa gacattattg tcatttgaag cacagattag
     4021 cttgccaact ggtaggccga tttgtttggc ataaaaggca gccaagatat ttccaaagtt
     4081 tcctgttggt actgtgaagt taaccttttc accagctaca atttcaccag tcttaaccaa
     4141 ttgagcgtaa gcataaacat aataaacaat ttgtggcacc agacgaccaa tgttcataga
     4201 gttagctgat gaaaattgca acttgttggt agtcaatttt tcacgaagag ccacgtcgtt
     4261 aaacatgtgc ttcacatttg tttgcgcatc gtcaaaatta ccatcaatag caataacatg
     4321 agtattgtca ccattctgag tggtcatttg caactcttgt accttgctga caccaccctt
     4381 tggataaaag acgataatct cagtaccagg cacatccgca aaccccgcca tagcagcttt
     4441 ccccgtgtca ccagatgtcg ctgtcaagat aacaatcttg ttctccaaac catgtttctt
     4501 agcagcagtc gtcataaagt atggcaaaat agacaaggcc atatccttaa aggcaatcgt
     4561 tgaaccatgg aaaagttcca aattgtattg cccatctaat ttcactaatg gtgcaatagc
     4621 tggagtatca aatttgctat cgtaggcatt gttgatacag tagtccaact cctcagctgt
     4681 aaagtcatct aaaaatgctg acaaaactag cttagcaact tcctggtaag aagcatcttt
     4741 caatttgtca aagtccaaat ctacctttgg ataagtaacc ggtgtaaaca aaccgccgtc
     4801 cgtcgccaaa ccttgcaaaa ttgcttggct ggcagttact gtattgttgg catcacgcgt
     4861 tgattgataa actaatgtca ttactctcta tcctttatct atagtctcgt ccattatatc
     4921 atgatttttc agaaaattct ccctttataa gagaaattat aggctcaatt cttctctcaa
     4981 aggctgtaaa taggtcattt cttccttatt tctcaccact agtccctcct cagctagtag
     5041 gagtaagtct ttggaaaatt caataatcgt agtttcttcc tcatctgtaa gatttttctt
     5101 agaaaattgt ctccttaaag acttgtaatc ataaccaaat actttaaaga aaggtgctgt
     5161 ttctaagtaa gcttctaact tgtctaaatt aaccaataat cccaagtgaa aagctgcaga
     5221 agcaaaagtc ctatcaagtg gctgtgtaca cacactacga aactcaactg ttcctcgagt
     5281 cgttaaatct tggtactggt aactacgatg agtttcaaaa tccttctctt gggggtaaat
     5341 aataacctca tccccattca gagcaaatgc ttggatttcg gacgtagcca aatagtcccc
     5401 agcctgaata ggataaaaat aataggtctg cccatcacgt tccgcagtaa aaatcgcaga
     5461 atgatttaga tagtcaaaaa aatcagcttc atcattaagg agtctagcat tgaccccaac
     5521 attctctgga tagataccat gcatagattc ttcccagaaa atatcccttg aaattttcgt
     5581 atcccaatcc gcacccgaaa attcagagtt tgcaaataaa taagccttag ccgcttcaat
     5641 ttgagtaaaa gcattaatca cccgtaagta gttggttttt gaaatatcca gctgaacctg
     5701 gctcccacag ataaaagtac catattcagg gaaatgatgt aaatctgatt taataatatt
     5761 tctactcaaa ttcaaataat ccatcaacat ctgatagcgt ggataagcca ctggacaatt
     5821 ctcattttta tcccagttgg gatggatacc acagccaaca atagcatgat ttgattcagc
     5881 taactttctc tgaattacat tcatataatt attgaaacga ttttcgacct cttgaatcgt
     5941 ttcagcctta ccaaatgcaa actcaatcgt tgtataggaa acttcaaata aaatagcatc
     6001 ctgacttatc ggatctacta actggatcag attgccaaaa tcatctacct ttgctacggt
     6061 gagatccaaa gtagaaacta aataatgaaa tagatccttt ataacttcaa catctgtagc
     6121 atccccttct aaacttgcaa caggatactc caactcaatt ccgacaaaca attcaggatt
     6181 ttctttaata tttttcaaat accgatgttt taataaatcg atagaacaag acatactacc
     6241 ctacactttc ttaatctaaa taaaatttga ataactataa ttataccaaa aataatataa
     6301 aaaaggaacg aagaataaca acgttcctaa cactatacta taccggcggc cggggtcgaa
     6361 ccggcacgtc cttgcggaca ctggattttg agtccagcgc gtctgccaat tccgccacgc
     6421 cggcaaatag taactggggt agctggattc gaaccaacgc atgagggagt caaagtccct
     6481 tgccttaccg cttggctata ccccaataat ataaaatagg cgagtgatgg ggatcgaacc
     6541 cacgcatgcc agagccacaa tctggtgtgt taaccacttc accacacccg ccataattct
     6601 attaacacgg gcagtaggaa ttgaacccac actgaaggtt ttggagacct tagttctacc
     6661 tttaaactat gcccgtaaaa tggaagggga gggattcgaa cccccgaacc cg
//
LOCUS       DAVBHS010000110         6672 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVBHS010000110 DAVBHS010000000
VERSION     DAVBHS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.110"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(251..511)
                     /locus_tag="VQ651_001586"
     CDS             complement(251..511)
                     /locus_tag="VQ651_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754555.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
     gene            complement(570..1121)
                     /locus_tag="VQ651_001587"
     CDS             complement(570..1121)
                     /locus_tag="VQ651_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754556.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(1111..1308)
                     /locus_tag="VQ651_001588"
     CDS             complement(1111..1308)
                     /locus_tag="VQ651_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754557.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(1312..1896)
                     /locus_tag="VQ651_001589"
     CDS             complement(1312..1896)
                     /locus_tag="VQ651_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754558.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(2304..2729)
                     /locus_tag="VQ651_001590"
     CDS             complement(2304..2729)
                     /locus_tag="VQ651_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754559.1"
                     /translation="MTSRVFIDADCISAFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNYDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(2722..3216)
                     /locus_tag="VQ651_001591"
     CDS             complement(2722..3216)
                     /locus_tag="VQ651_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754560.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMVLGHYIKSTEQLLENNRISQGKYEELLLDAFRYDIVYGLDEEGE
                     LSFD"
     gene            complement(3262..3468)
                     /locus_tag="VQ651_001592"
     CDS             complement(3262..3468)
                     /locus_tag="VQ651_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754561.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(3455..3616)
                     /locus_tag="VQ651_001593"
     CDS             complement(3455..3616)
                     /locus_tag="VQ651_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2754562.1"
                     /translation="MNMINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(3981..4937)
                     /gene="lytA"
                     /locus_tag="VQ651_001594"
     CDS             complement(3981..4937)
                     /gene="lytA"
                     /locus_tag="VQ651_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HET2754563.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPDFTVEPEGLITVK"
     gene            complement(5313..6665)
                     /locus_tag="VQ651_001595"
     CDS             complement(5313..6665)
                     /locus_tag="VQ651_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET2754564.1"
                     /translation="MDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVGRFLGQE
                     SLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWILGALLSI
                     LVMLLGFLGLYHLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLRSIGDSL
                     AALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRKSVPELL
                     PQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQTAARRI
                     MTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIFLFFASP
                     ALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPLVSSFIE
                     LIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAILATKVQS
                     "
BASE COUNT         2138 a         1393 c         1047 g         2094 t
ORIGIN      
        1 atttgtcctg ttctcgtttc atttcactat actaaaaaac cacctgaaac aaatcaagtg
       61 actagaggat aaaaacaaag actaggcaaa aagttttgaa aagcaaaaaa cgcataatat
      121 caggtcttga aaaaccttga tactatacgt ttgattgtgg gaagattgac ttcattttct
      181 cctgaaattg aagttttttc tagtttccag atatagacga attcaatata gccatctcgc
      241 tacaattatt ttaaccatcg agaaaatttt tcagaatttc aaaaatttca ttttgatcca
      301 atgaaatgtc atcattttga agaatagttg acttcacagg ctctaattct atcacaaagc
      361 ggtgtatgtc tgcttttacc gcactacttg atgttatagc cgataacttt tcatctccta
      421 gcaaggaagc tgtcaaacgg gtaaggtcat tcaaatgctt tttaatggac ttacttaaac
      481 cttgagcacc tgtagcagag cgttccctca tatccagcca agctttagcc ttaaaaataa
      541 ttaatactca atgaaaatca aagagcaaac taggaagcta gctgcaggct gctcaaaaca
      601 ctgttttgag gttgtagatg gaagctgacg tggtttgaag agattttcga agagtataaa
      661 ccacaattac tcaataccga atacccctga atggtttctt tttcatgcac caatatatta
      721 taataatctt catccaataa taaggctgat aaactagcat cttggtcaaa atgtaaggga
      781 atttctcgac cgtccttctt taatggatat tctggtaaga tactaaatag ttcaatcata
      841 gaaggaaact caggattagt tgttgtaaat cgaaaaagct gcgctttctc atctttctga
      901 cttccttgat actctcccaa ttctaaaaaa tgattcaagg tagtataaaa ttccttattt
      961 tttacttcat aaatgatgac catatcataa tcttttgttg tgcgactttt aaatccttgc
     1021 gaatccaata cgatagaggt agcagttccc ccaatcagaa cataatagtt ctgaaaatcc
     1081 gcaaacattt cttgaaaaat aactttcgta ttagctggca tcatcttctc ccagatactg
     1141 taatatcata ttttctagtg cttcactctc ttcctctata cgtgggtcat catcatcttt
     1201 taaggtcaaa taaagagaaa tcggatctac aaattgttta tcatgattat ttttaaaatc
     1261 attccaaaac tcagatacaa aaggacgata tttccatatc tctagcatct ttcattttaa
     1321 aacatgctga gaaagtggca aggataactg attgaatttt ctctgccata tgacatagct
     1381 agtattttca tccgtttcag ctaaaaaagt tgaatgcgac aaagcataag caccaccata
     1441 tagaaggtta gaaacagatt ttatctgctt tatatcgcca tctggcaata aaaaggcaat
     1501 ccacgttaat tgttcgctag gtgttaattc cttagggatt tcagtatcat tcgcattgag
     1561 tactagtccc aatggaggga agaagaggtt tcccttaaag tctacaaacg gaactctagc
     1621 ttgaagtaat tgcttttttt cactgtctga taacttcgaa aacaccaaaa caacatccat
     1681 attggctttt tcacccatag tgcgagcctg agtaacaaat gaactcaaac tcccccttct
     1741 cttttcttta atcaataaaa atgattcctt atgaaaagtc atttgagtat actcaaacct
     1801 ttttacaaaa ctgattggta aagtcaaagt cagttcctca atactgacat caacataaaa
     1861 ggattgaaaa attgacatta tctttttgac attcatgttt tttccttatt ctatcatttt
     1921 tattatattt tatcattcgg tataataaaa tataagtttt ttgataaaat aaccaaaaac
     1981 tactcgctat cccctctcac attaccctac atatcgtttg acatgcgact gatagttcag
     2041 gaaaacttca aggagaactt ttctctcatc cactatgcag gacttactat ttcacttcta
     2101 ctcctatagg ctcaatttga cacttttctt tttcgaattg ctcatatgcc tcctgaatag
     2161 tgagttcgat ttatccaaat ttatttttgt ctattttgat gaatacttcc acgaagatag
     2221 tctgaaaatg aatttgcacc aattttcctt ctctttttaa gcatattatt ccagatatga
     2281 ttgccctctt gttcagtaat aaattacgct ttaaacgctt caatcagtat atctcctgtt
     2341 gtcatatgtt ctaaagaaaa ttcttctaca tatggtttaa catctcttag gttattactt
     2401 cctaatatcc cattatgctt tttcgctaag aaaatagaag tcgcttctcc cttaccaata
     2461 atcttgttac tatcataatt tcttgttaaa tctctatata atgcgtattc ttcagttcca
     2521 atgtctatgc tcacaatctc agctgaaccc ttagttacca actggtctat cctagatttt
     2581 aaatggggaa ttgtaggtat attgatttca tcatacacct cttgtggaat aacaatttta
     2641 cccgaataga gcttttctaa aagatgttca gtgccaaccc ataaaaatgc tgaaatacaa
     2701 tctgcatcaa taaatacacg actagtcaaa cgacaactcc ccctcttcat ctagcccata
     2761 tacaatatca tatctgaaag catctagtaa cagttcctca tacttccctt gcgaaattct
     2821 gttattttct aaaagttgtt cagtcgattt aatatagtgc cctaatacca tttccttttt
     2881 actttctgac aaaggacgat ataaacttgt atcatagcct aatcttgaag ctgtctctat
     2941 aacactaata tccatatttt taatttcttc tgcatcaagg tatccatcat ttctcaatct
     3001 atataacata gctttatgac tgataccata aaactgaccc aattttataa tatcttctac
     3061 ttcaagatga gttctattgg cattttctct gatttcctca accatcctat acagtgaaga
     3121 tgggaaaatt aaaaaataag aagcaaactg atccgctttt ctttcagttt catctccttc
     3181 accaatcaag ataagactga ctgaactctt cttcacctca tcataataaa gatgatacag
     3241 tccatgtgct aaagaaaatc tttaccttcc taatggcatg tctgaattga ctgcaatgag
     3301 actgaactga gttcctttat aacagactcc gctaatattc tttccgagtc cataaaatac
     3361 cagcgtcaaa ttttctatct tttgtaccaa tttaaaaata tctatcggcg attcaccatc
     3421 agctcccaac ttttttctaa gatttgaagc tttattactt aaatccattc gtgatattcc
     3481 tttcaccttt ttccattttg gcaatcatgc tttgattcaa agacaaatac tcagcaatct
     3541 tatcttgagt aagatttgaa gatattctaa gttcttttat tctttttcca acatcacata
     3601 catttatcat attcatacac ctctcataaa aagaatagca cattcttgtc ataatttttt
     3661 aaataaaaaa attatgacaa aacaaggaag caatttattg atgctactta aaaatctaaa
     3721 attgatgata ttaaacctat ttgatgagtt cctatcaaaa atcgtatctt caacctcaaa
     3781 acagtactta aagctatccg actcggttta cattgtcaaa tttagatttt atttgagcat
     3841 aactttctag tttgcttttt gatttttgtt taatatagta acaaaaattg aaggaaatat
     3901 ctccacaaga aaacgcatac tattaagctt tttcaagacc taataatatg cgctgttctg
     3961 atttgaaaga cattccatta ttattttact gtaatcaagc cttcaggctc tactgtgaag
     4021 tctggcttgt ctgccagtgt tccgtctggt ttgaggtagt accagcctgt tccgtccgct
     4081 gactggataa aggcatttga taccatggcg ccttctttag cgtctaagta gtaccaagtg
     4141 tccttgtact tgacccagcc tgtcttcatg gcaccttctt cgttgaaata gtaccactta
     4201 tcagcgattt tcttccagcc tgtagccatt tcgcctgagt tgtcgaacca gtaccagttg
     4261 ccgtctgtgt gcttcctcca gcggtctgca agcatatagc ctgaactgtc aaagtagtac
     4321 caagtgccat tgattttctc aaacttgtct tttggataag agccgtctga atgtacgtac
     4381 cagtagccag tgtcattctt ctgccagcct gtttcaatcg tcaagccgtt ctcaatatca
     4441 tgcttaaact gctcacggct aatgccccat ttagcaagat atggataagg gtcaacgtgg
     4501 tctgagtggt tgtttggttg gttattcgtg caatactcgt gcgttttaat tccagctaaa
     4561 ctccctgtat caagcgtttt cggcaaacct gcttcatctg ctagattgcg taagagttcg
     4621 atataaaggc ggtagtccgt catgaactct tctttggttg aatggctttc aatcagttca
     4681 accgctgcat aggtctcagc attccaaccg cccccaacgt cccaggcacc attatcaaca
     4741 ggtcctacct gcatgatgca accgttccca acaatgtgcg agaaaaaacc taattctggg
     4801 tctttccgcc agtgataatc cgcttcattc tgtacggttg aatgcggatt cccagttgag
     4861 tgtgcgtgta cttgcctata tggttgcacg ccgacttgag gcaaatctgt tcttaattta
     4921 ctcacattaa tttccatatt ctactcctta tcaattaaaa caactcattt tttacaatcc
     4981 agaaccagaa actcctttat ttctacctta caaagaagac aatcttagtc acgattaagc
     5041 ttgtagatag aacctcaaaa cgcactattt tgacactgta aataggactg acaaggtctg
     5101 cattctatct acaataacac cccagactaa aaagcttttc aaaagtatat ttttacagtc
     5161 tctatatgtc cttttcataa atactatact ttattatatc acataaaaga agtcaaaagt
     5221 ctgttaaact attttcaaca ccaaactaaa gaagagaaca caagtttttt cgatgttcac
     5281 tagaggaaat ggattttatt cagtaaatcc aactaggatt gcactttggt tgccaaaatt
     5341 gcctttcctt cttttatcaa gggatgacgg aatagtgaga agtacagttg aactgtcatg
     5401 gcaacccaga taagaggttc acaaaggata acacccttat atcctgccca aggaataatc
     5461 aaaaccacaa aaacgatttt tccgattagt tcaataaagc tagaaactag aggaaggatc
     5521 ttctgcccca agccctgcaa gcaattgcga taaatcaaca agaggctcaa aatgggataa
     5581 aaggctgaac tgatttgcag atagagactt ccattttcta tcaagtaacc atctgtcgaa
     5641 ctagccaaga aggaaaccaa ggctggactg gcaaaaaaga ggaaaataca aacaaaaact
     5701 gcccaggata tacttaaacg actgccgatt cgaagacctt gaacaatgcg gtcaggtcgc
     5761 ttagctccta gattctgaga agcaaaggtc gtcattgatg cagaaatagc ggtcatagga
     5821 agaagggcaa aggtcataat gcgtcgagct gccgtctggg cactaataat cactgcacca
     5881 aatgtattaa cagaaaactg taaaatcaca ctgccgatag atacaattga actcatcaag
     5941 cccatagcca aaccttgctc caagagatcc gcgtacaagc ttttgtccca tttgaaatgt
     6001 ttaaactgtg gcaagagttc tggcacactt ttacgaatat aataaaagca gagaaccgct
     6061 gataaacctt gcgaaataat ggtagcaagt cctgcggatt gaactcccag atgcaattgc
     6121 gtaataaaat agagatccag aaccacatta accaaggcag agaaaatcag aaatcccagg
     6181 gctgctagac tgtcaccaat agaccgcaac aagcctgcaa aaagattata agcaaagctg
     6241 acacctacac aggtcacaat catagaaata tattgataag attgaggaag aatttctgca
     6301 ggagtatcta agtattgcaa gagatgatac aagccaagaa agcccagcaa cataactaga
     6361 atgctcaaaa gagcacctaa aatccaagta gctgctactg cttccttgat tttagtgaaa
     6421 ttccgagccc cataataacg agcaatgaca atccccatgc cattgccaac accaagtgta
     6481 aaacctacaa tcaggtcaaa aatcgctgtc gtcgctccta ctgcagccaa ggattcttga
     6541 ccaagaaatc gtccaacaat caagacatca gcagtgttat agagctgttg aaaaatattt
     6601 gatagcaaaa ttggaaaggt gaagcttaag agcgagggaa gaatcggacc atgtatcagg
     6661 tccactgttc gt
//
LOCUS       DAVBHS010000111         6633 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVBHS010000111 DAVBHS010000000
VERSION     DAVBHS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.111"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(84..1244)
                     /locus_tag="VQ651_001596"
     CDS             complement(84..1244)
                     /locus_tag="VQ651_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2754565.1"
                     /translation="MKKKFALSFVALASVALLAACVEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTNMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            complement(1345..1593)
                     /locus_tag="VQ651_001597"
     CDS             complement(1345..1593)
                     /locus_tag="VQ651_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HET2754566.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            complement(1672..2262)
                     /gene="clpP"
                     /locus_tag="VQ651_001598"
     CDS             complement(1672..2262)
                     /gene="clpP"
                     /locus_tag="VQ651_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HET2754567.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            complement(2436..3065)
                     /gene="upp"
                     /locus_tag="VQ651_001599"
     CDS             complement(2436..3065)
                     /gene="upp"
                     /locus_tag="VQ651_001599"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET2754568.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(3154..3621)
                     /locus_tag="VQ651_001600"
     CDS             complement(3154..3621)
                     /locus_tag="VQ651_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HET2754569.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            complement(3640..4197)
                     /locus_tag="VQ651_001601"
     CDS             complement(3640..4197)
                     /locus_tag="VQ651_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET2754570.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            4322..5167
                     /locus_tag="VQ651_001602"
     CDS             4322..5167
                     /locus_tag="VQ651_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2754571.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEDGGLLM
                     GYEID"
     gene            5226..6398
                     /locus_tag="VQ651_001603"
     CDS             5226..6398
                     /locus_tag="VQ651_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET2754572.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSNPI"
     gene            6449..>6633
                     /locus_tag="VQ651_001604"
     CDS             6449..>6633
                     /locus_tag="VQ651_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET2754573.1"
                     /translation="MEHIILLRGVTPNGKNAIPKMSYLVDILTEADFQQVRTYIQSGN
                     IILESNLALEEIREQVHT"
BASE COUNT         1893 a         1384 c         1287 g         2069 t
ORIGIN      
        1 agtttgaaga gattttcgaa gagtattaaa tatcgaaaca gggagtgagt caaaggctca
       61 ttccctattt caacattttt ctattatggt tttacaactt ctgctgcttc aactttacca
      121 ttgttcatgg tcatcatgta agcagttttg actgtgttgt ggtctgcatc gaagcttgtt
      181 tgaccagtta caccttcaaa atcttttgtt ttagcaaggt tatccttgat ttcacctgaa
      241 tttttagcac cttttgctgc gtttgctaca aggtgaactg aatcataagc caaggctgca
      301 aatgttgaag gctcttcatt gtacttagca cggtaagcgt caaggaaggc tttagcttta
      361 gctgaaactt ctacagtagt tgagaagcct gagataaagt agatgtttga tgctttttca
      421 gcagttgctt gttgtacaaa ctcctcaccg ttgaatccat caccaccaac gattggtttg
      481 tcaattccca tgccacgcgc ttggtttaca atcttaccag cctcattata gtaaccagga
      541 acaacgatag catcaaagtc tttccctttc atgtttgtaa gggctgcttg gaagtctgtg
      601 tcacctgcta cgaaagtttc atctgcaacg atttcaccct tgtatgactc gcggaaagat
      661 ttagcaatcc ctttagcata gtcactggca ttgtcagtgt aaagaacaac tttcttagca
      721 tttaattttt cagaaacata gtttgagata atttttcctt ggaagctatc ttggaaagtt
      781 ccaataaaga ggtaatcttg acctttagtc aatccatctt gagtcgcact tggtgagatc
      841 aatggaacac ctgcttttgt agcgttcgct accgcagctg cagtcgcacc agatgtcgca
      901 ggtcctacga ctgctgatac tttagattgg gttacaaggt tagttgtaac tgaagcagcc
      961 tcagctgttt cagacttatt atctttatcg actacttcga tttgttttcc atcgatacca
     1021 cctgcggcat tgatttcatc aacagccaat tgggcacctt tttgttcagc tgttccgtat
     1081 gcagctaaag aacctgattc ttcaaagtta aacccgattt taattgtctt ttcctctact
     1141 gagttaccag cagtgttgac tgctccagac ttcacttcta cacaggctgc aagaagtgct
     1201 acacttgcaa gcgccacaaa cgatagggca aattttttct tcatcttttt gtctccttat
     1261 ttcttaaata gtctgttaag aatataccga attatcagaa aattgtcaat gttttccttg
     1321 aactttttcg atgataacgt tttcctatcg ataaagattg cccacaaagg gtgtttccaa
     1381 ctcttggata tgacaaaccc taactttttt aataaatttt tccttgctca agcgtccgac
     1441 caattgctcc acttcttgag ttggaacata gagttgtaag taacgatgtt tcttggaatg
     1501 ataggtaata tctccataat cctgcagttt tttggcatca cgattatagt aaagatagat
     1561 aatcaagcca gagcgattga ctttttcaaa catgataaat cctctttcta agaaatctct
     1621 ccctattata ccaaaaaaag caaacccagt cgagtttgcc ttctatcatc attagttcaa
     1681 tgaattgttg gccataattt catcaataaa gccatattca agtgtttcct gggcgctcat
     1741 ccagttatca cgttctgcat ctgcatggac tttttccatt gactgacctg aattttcagc
     1801 caagattttt tccaaggtat tacgagtttt gagcaagtgt tctgcagcga tagccatatc
     1861 agtttgttgg gtaccaccac ctgtaccgcc cattggttgg tgaatcatgt attcagcatt
     1921 tggaagcatg aaacgtttgc cttttgctcc acttgatgcg atgactgttc ccatagatgc
     1981 agccattccc ataacaatgg tttggacatc tgccttgata aagttcatgg tatctacgat
     2041 tgccaaacca gctgaaacag aaccacctgg tgtattgaca taaaggtaaa tatcttttgt
     2101 actatcttgg gcatccaaga aaagcagttg ggcaataaca gagttagcca tattgtcttc
     2161 aaccggacct gtcagcataa tgatgcggtc tttgagaaga cgtgagtaaa tatcgtaaga
     2221 acgttctcca cggcttgttt gttcaataac tacaggaatc attcatttct ccttttgagt
     2281 tttaattttg ttggtcaaat gactgaagat aagactatta taatatcttg gtcaaaaaag
     2341 gtcaaatttt tgctctgctt tcattagaca gaaacaaaaa cccaacctcc tttcgtgact
     2401 ggaaatactt ttccaagtca ttcttctttt cgatcttatt ttgtaccgaa caagcggtct
     2461 ccagcatctc caagacctgg aacgatataa ccgtgttcgt tcaaacgttc atccaaggct
     2521 gctgtaaaga tttctacatc tggatgagct tcttgaaggg cttttacacc ctctggagca
     2581 gatacaaggc agacaaattt gatatttgat gcgccacgtt ttttaagaga atcaacagcc
     2641 aagattgctg agccacctgt tgccaacatt gggtctacta caaaaatttg acgttggtca
     2701 atgtcctcag gcaatttcac caagtattca actggttgaa atgtttcttc atcacggtac
     2761 ataccgatgt ggccaacttt agcagctgga accaagctca agagaccatc aaccatcccg
     2821 atacctgcac gcaagattgg gacgatggcc aatttcttac ctgccaattg tttttgaact
     2881 gtttttgtaa ttggtgtttc gatttccaca tcttctagtg gaagatcacg aagtacttca
     2941 taccccatca acattgcaat ctcatctact agctcacgaa aagcttttgt agaagtatct
     3001 gtacgacgca agattgacaa tttgtgttga atcagtgggt gattaataac ttcaattttt
     3061 cccatttttg gaattccttc tttcaattta ttcttcttat tataccaaaa aacgggttaa
     3121 aaatctttct aaaccattta tttttgataa tttttacatt agatcagcct ctttaagagc
     3181 tgtctgtact gtctcaagtg gtaaatgggt caattctgtc cctttttctt gataaaggta
     3241 ttgggcgtag tcgtccattc ggtactggtt gatataaacc acacgcttgc agccgacctg
     3301 aagcaattgt tttgtacagt tgagacaagg aaaatgggtt acataggctg taaagccttt
     3361 gggaacacca cgctcagcac cttgaaggat agcattgacc tcagcgtgaa gggtgcgaac
     3421 acagtggcct tcaatgacca aacattcgtg atcaatacaa tgctcagtcc ctgacaccga
     3481 accattgtaa ccagtggaaa taaccttatt atcttttacc agaatcgcgc ccactttagc
     3541 acgtttacaa gtggaacgat tcgcaattag tagagcttgg gctgcaaaat actcatccca
     3601 ggccagtctt ttttcagtca tctcttttct cctttttctc tattttttaa aaaatggtaa
     3661 acctaaatct gcaatctttt cagctggtac cttcatgcca tccttgatcc attttagaag
     3721 gacagagacg atggctgagc tccagaagga atgaagataa gagctgacac cttttgattt
     3781 cccatggtat ttttctagaa attcctgcat ggcttggaca aagatttttt ccagatggta
     3841 atccaaggcc aattgaatta ctctagcttc ctttctggcc tcccggaaaa ggtgaaccca
     3901 aaccaaataa aggtctgtct ttaaatcgta atgatgcagc tgttccataa tattgtggac
     3961 agttcgttta aagacgctct ctaaaatttc ctctttggag tcataattgc gataaaaggc
     4021 cgcacgcgaa acacctgcac gtttgaccaa ttcagaaata ctaatcttgg tcagttcctt
     4081 tttttccaag agttgcaaga gggctgtttc aatggcttct ctggttaata aattggattc
     4141 ttggtttgat tttctgagat tttcaagaga cttttcagag attctacgtt cagacataac
     4201 attttctttc tacttgtcac aacagacgga tgatgctttt gtttcaaggg ttttcatgat
     4261 ataattgtaa aaaaagacag aacctttgtc aatgtataac tgacaaagat ggagaatttc
     4321 tatgacttgg aagattattg ctgactctgg ttgtgattat cgtcaactgc caacaccagc
     4381 tattaacaca acctttgtaa gtgtcccctt aaccattcaa gtagctgatc aggtctttgt
     4441 tgatgacgcc agtctcgata ttgaccaaat gatggaaacc atgtatgcaa ctgcagaagc
     4501 ttcaaaatcg gcttgtccaa gcccagatga ctatttgcga gcatttgaag gtgccaaaaa
     4561 cattttccta gtaaccatca caggtaccct ttctggcagt cacaatagtg ctcaactagc
     4621 aaagaatatt tatctggaag accatcctga cactaagatt catgtgattg atagtttatc
     4681 tgctggtggt gaagttgacc tactcgtaga aaaattgaat gacttgattg accagggctt
     4741 gtcttttgaa gaagtggttg aagctatcac cgcctatcaa gaaaaaacta agttgctctt
     4801 tgtcctagcc aaagtcgata acttggtgaa gaacggccgt ttgagcaagc ttatcggtac
     4861 ggtcgttggc cttctcaaca ttcgtatggt cggaaaagct agtgaaactg gaactctcga
     4921 attgctacaa aaagcaaggg gatcaaagaa atcagttcaa gctgcctatg atgagttagt
     4981 aaaagctgga tatgctggtg gccgtattgt catggcccaa cgcaataacg aaaaatgttg
     5041 tcaacagctc tcagagcgaa tccgtgaaac cttcccacaa gcggatatta aaattctacc
     5101 aacctctggt ctctgcagtt tctatgcaga agatggcggt ttgctgatgg gctatgaaat
     5161 tgattaattg tattcttgac aagaggtgac catcatctct tgtttttttg tggtacaata
     5221 gagctatgaa acattttgat actattgtca tcggtggggg acctgctggt atgatggcta
     5281 cgatttccag tagcttttat ggacagaaaa ccctcctcat cgaaaaaaat cggaaacttg
     5341 gaaaaaaatt agctgggact ggtgggggac gttgcaatgt gaccaacaat ggtagcttag
     5401 acaacctgct agctggaatt cctggaaacg gacgctttct ttacagtgtt ttctcccagt
     5461 tcgataatca tgacatcatc aactttttta cagaaaatgg tgttaaactt aaggtcgaag
     5521 accacggacg cgtctttcca gccagtgaca agtctcggac tattatcgaa gctttggaaa
     5581 agaaaatcac cgaactaggt ggtcaagttg ctactcaaat agaaatcgtt tctgttaaaa
     5641 aagtagatga ccagtttgtc cttaagtcag cggatcaaac cttcacttgt gagaaactca
     5701 ttgtcacaac aggtggtaag tcttatcctt cgactggttc gactggtttt ggtcacgaga
     5761 ttgctcgcca ttttaagcat accatcaccg atcttgaggc tgctgaaagt cctttattaa
     5821 cagattttcc acataaagcc ttacaaggga tttctttgga cgatgtgacc ctaagttatg
     5881 gtaagcatgt catcactcat gatttactct ttacccactt tggtttgtca ggtcctgctg
     5941 ccctacgcat gtctagcttt gtcaaaggtg gggaggttct ctcactcgat gttttgcctc
     6001 aactttctga gaaggacttg gttacatttc tagaagaaaa tcgggaaaaa tccttgaaaa
     6061 acgctttaaa aaccttgtta ccagaacgct tggccgaatt ttttgtacaa ggatatcctg
     6121 aaaaagtcaa acaactgact gaaaaggaac gagaacaact tgtccagtcc attaaagaac
     6181 ttaaaattcc tgtaactgga aaaatgtccc ttgcaaagtc ctttgttacc aagggtggag
     6241 tcagtctcaa ggaaatcaat cctaaaaccc ttgaaagtaa gctggtacct ggcctccact
     6301 ttgcaggcga agttatggat atcaatgccc acacaggtgg ctttaacatc acttctgccc
     6361 tctgtaccgg ctgggtggcg ggatcaaatc caatctaaat atacattact aaatggaaca
     6421 atcccccaaa aaaaagaaag gaactacttt ggaacatatt atcttgctaa ggggagtcac
     6481 tcctaatgga aaaaatgcta tccctaaaat gtcttatcta gtagatatct tgacagaagc
     6541 tgattttcaa caagttcgaa cttatattca aagtggaaat attattcttg aaagtaactt
     6601 agctctcgaa gaaatacgag aacaggttca tac
//
LOCUS       DAVBHS010000112         6534 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVBHS010000112 DAVBHS010000000
VERSION     DAVBHS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6534
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.112"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(865..2091)
                     /locus_tag="VQ651_001605"
     CDS             complement(865..2091)
                     /locus_tag="VQ651_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HET2754574.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVFEVGPGDEVIVPAITYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(2134..2826)
                     /locus_tag="VQ651_001606"
     CDS             complement(2134..2826)
                     /locus_tag="VQ651_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET2754575.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGNLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSTEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFKVLK"
     gene            complement(3012..4763)
                     /locus_tag="VQ651_001607"
     CDS             complement(3012..4763)
                     /locus_tag="VQ651_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754576.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALVDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(4753..6498)
                     /locus_tag="VQ651_001608"
     CDS             complement(4753..6498)
                     /locus_tag="VQ651_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2754577.1"
                     /translation="MSIIQKLWWFFKLEKHRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSPAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTELRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSEVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILNPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGRHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
BASE COUNT         1971 a         1414 c         1376 g         1773 t
ORIGIN      
        1 gtcctattct tatttcattt tattatatct acaacctcaa agcagggttt tgagctatct
       61 atggccaact tcctagtttg ctctttagcc ttccattcaa aagtgatgct atctttctta
      121 gcttgaatat aaaatatccc atttactata tttaaatttg ttgatatgtt gtaaattata
      181 aatgaaagaa caaatatctt gtcatacaag cattcgccag ggatatttac atgaatatcg
      241 ctcatatggc ggattgaaac taaaacacta caatatattt cttaaataga ccttctataa
      301 aagctagatt taacctcgat aaaaatagat gcagtgcttc taaaatactg gaatagggca
      361 tataggtaat cgtagacaag tgttttatag attgtctcgc gggagatcta atgatgtata
      421 gcaagcatca ctaaacatat agaatatttg tgttattata atgtcaatat gctataaatt
      481 ctatcatgca aaagaatgac tagaacatga aagacaggca caaaactttg aacaatattc
      541 tgaaaacaaa aaaagacgcg agtttccaca atctcactca tggaaactca cgtctttcaa
      601 aaaatagggt gaattaaaaa ctagaacaag acactagatt ttctaaatga tgtctagtaa
      661 ggattctaaa tgctacacca aattgctcac atcttatttc aaaccacggg actcccacaa
      721 gaaaggaaaa tcctataaaa attctctctc tgaggagaga gaggcctcaa ctacaggtgt
      781 aaactattat caattgaaaa caacagtttt tacactcaat tagacttttt agggacagga
      841 tcactttcgc ttgactgtag tttgtcattt ttttgataaa gttagcactt tttcagaaac
      901 tgttttgaaa gtctcaatga tatagtctac ttcttcatcg cttaatttag tatgaagagg
      961 gagggtaatt tcattctcaa agaaggcata ggccttagga tagttcgtca tatcaaatcc
     1021 aagattctta taggctgtca agagaggaag cggtttgtag tgaacattac ttgcaattcc
     1081 tgctttagcc aattcttgga tgatgaggtt gcgttcttct aggcttgctc cttctacacg
     1141 ggtgatgtag aggtggcgtg aagattcgac agtttcagtc ttgtgtgcca aaggatggat
     1201 gcgagaacct gcaaaaccac tatcatagcg gtccacaatg tccttacggc gttgcaacaa
     1261 acttggatag cggtccaatt gtaccaaacc aagtgaagcc atgatatcgg tcatgttgca
     1321 cttataggct ggtgtaacga tatcgtattc ccatgacccc agttgcatct tggcaagagc
     1381 atccttagtt tgcccgtgaa gggaaaggat ttggaattcc ttgtacatct cttcgtcatc
     1441 aatcactgga ttggctttcc aagtcgcact tccaccttct gccgttgtaa agttcttaac
     1501 ggcatggaat gagaaggaag taaagtcagc gatagaacca gaaggttgtc ctttataagt
     1561 agatcccaaa gcgtgggcac tatcagagac aatgacaata cggttaaagg ccttttgcca
     1621 cttgcttgaa gcggtaaaga agtcacgttt tttctccacg acttggaaca aacggtcata
     1681 atcgcaaaca atccctgcga gctctactgg gataatcacc ttagttttct cagtgatagc
     1741 ttgctcaagc aggtcatagt ccatctcaaa cgtatctgct tggatatcca ccatgacagg
     1801 ggttgctccc acgtgcgtaa tgacactaca tgaagccgta taggttatgg ctggaacgat
     1861 gacttcatca ccaggtccca cttcaaaaac gcgtaaaatc aactccagag cggctgtcgc
     1921 agagttgaga caaacagtct taggtgtctg tgtgtaaaga gacaagcggc gctccagttc
     1981 ttttgtttta ggacctgttg tgatccaacc agaacgcagg gtatccgcta cttcagcaat
     2041 ttctgcttct gtgatatcag gcggtgaaaa tggaatattg taatttggca ttgatttgct
     2101 ccttttatct gtactcattc tcttatgact actttatttt agtaccttaa acacggtttg
     2161 aaacatgatt ttgatgtccc caaagaaact aaactctcgg agataggcga ggttatagcg
     2221 catcttttca ggaagaacat gctccacata ggcctgatca actgacagac ctttctctgt
     2281 catttgactg atgatggtgt cctcatcctt gtagttgatg ctggctggag aggtaattcc
     2341 tgcttgcaag agcaaggttg ccatcatttc agtgctatac tgctctgtat aacgtggcac
     2401 ttcaggtcgt gtaccgacaa aggacatctc acctttaagg acattgacca actgaggcag
     2461 ttcgtccaaa cggacacgtc ggatgaaatt tccaaccttg gtaatacggc tatcgttagc
     2521 agaagtcacc agatttcctt ttttatccgc atccgtcacc atggtacgga acttccaaat
     2581 cttgaaccga cggttgtact gggtcacacg ctcttgcttg taaatcactg gccccttgct
     2641 atccaacttg atccaaatgc tcaagatgag aaagatggga gaggtcagaa ccagtaagac
     2701 caaggccaaa acccagtcca agcaacgctt gaaaatcagc gaacccttcc ttttagagac
     2761 aagctggtag taagactcaa cctcgcttga tttcatttcc acaggcaagt cttcccattt
     2821 cagcatgctg ttctcctttg tttttattat actatttgtc aacacttttt gtcaacactt
     2881 ttcattatac cacaaaatgg aaaaggcggt gaaaaaaatc tgtaatttga aggatttcct
     2941 tctagtactc aatgaaaatc aaagtacagc tttgagattg cagataaagc tgacatggtt
     3001 taaagagatt ttcaaagagt ataggccatg gcccctgcct gcaaactata catcttgtga
     3061 taggttcctc ccagagccaa gagttcctca tgggttccac tctcgataat gcgtccctta
     3121 tccaagacat agatgcagtt ggcgtcttga atagtagaaa ggcggtgagc tatagcaata
     3181 gttgttcggc cctgtctcat cttcgccaga gaagcttgaa ccaagctttc tgtttcagag
     3241 tcaatattgg ctgtcgcttc atccaaaatc aggattttag gctggctggc gactgttcta
     3301 gcaaaggcaa gaagctggcg ttgcccagta gagaagctcg aaccacgctc ggaaacaggg
     3361 gagtcgtacc cctgaggaag ttcttgaata aaggaatctg catccacaaa ggctgccgca
     3421 gcctgaacct gctcatcact gatttcttgg tacatggcga tattggactt aatagttcca
     3481 tgatagagga agggttcctg caagaccaaa ccgatgtttt ttctcagctc ttcttgactg
     3541 aaatccctga tatccacatc atccaagaga actctccctg actggaattc ataaaagcgc
     3601 atgaggacat tgataataga cgatttccct gaacctgtat gacctacaaa ggcaatggtt
     3661 tcacccttat taacagagaa agaaatgtca tccagaatcg gatgtttacc gtcatatgag
     3721 aaacacacat gttcaaagcg gatattgcct tcttggactt tggcttgccc attttcttga
     3781 agaggttcat aggtcctctc gtctaccagg gcaaagacac gacctgcaga aaccatagac
     3841 gtttgcagtg ttgaaaagtt ttgcgtcacc tcaatcaaag ggtcaaagag gcggttgatg
     3901 tactggataa aagcatacat ggtcccggcc gttatcccga tagaaagacc acgatagcca
     3961 aaataggcca tcaagacagc ataacctaga agtttcaaca aactcatggc aggtctcaaa
     4021 aagagggcat ccaaggctac agaacggttg gcatagacca agtgctcttg gttgatttca
     4081 tcaaattctg cctgcaggcg cttctcttga ttaaaggcct ggattattct gattccctcg
     4141 atgttttcag ccagcttgct attgatatct gacaagagac ttctggtttt ctcaatgatt
     4201 ttcactgatt ttttccgata gagattgacc aaaaggaaaa tcaaggggag aaagagcaag
     4261 actaatgctg tcaaacgaaa atccaatacc aacatggtat aaagggttgt cagaaagatg
     4321 aaaactgctg agataaagct agataaaatc ccagaaaaca tatcactaat cgtctcggta
     4381 tcgttggtca aacgagaaac gatagaacct gctggcgtct tgtcaaagta agacatgccc
     4441 agtttctcca tattggcaaa ggcatcccga cgaatatccc taacaatact gtaagacacg
     4501 cgcgcaaaga gaagattgcc gacatactga actacagttt gtaggatgta gagaccatag
     4561 tagaccagca aaacggtaac ggctagttgg ttaagattgc tgagatactg gtcgataaag
     4621 tgggaagcca cgaggggtat gacactttta atgaccgtcg tcgctaggag aaaactgagt
     4681 gccaaaaagg tcaggagtct ataaggcttg agataagaca tcaagcgctt caatacagcc
     4741 cattgttctt gtttattctg catcttcttc tcctttcatt tctaactgct gagactggta
     4801 ggtttgggca taccagccat ccatcgctag caagtcttcg tgcctgcctc gttcgataat
     4861 ttgaccattt tgtagaacta aaataaaatc tgcatggaca acagcactga ggcgatgggc
     4921 agttataatg gttgtcttgt cctttcgcgt ctccttgaga ttgtcgataa tcgcatactc
     4981 tgtcttggca tctacggcgg ataaggaatc atccaaaatc aagatatcag ggtttaaaat
     5041 catagcccga ctcatagcca accgttgctt ttgaccacca gaaagactga ctcctttttc
     5101 accaatcagc gtatcaaatc cttgaggcat gtctacaata tcttggtaaa ctcgggctag
     5161 cttagtagct tcctcaacct ctgaaagggg caagttagga ttgccaaagc ggatattgtc
     5221 taggattgaa gtcgcaaaga gaaactggtc ctgaggaaca tagcccatga gactgcgaag
     5281 ttctgtcaaa cgatagtccc gaatatcgtg accgtttaga taaatggcac ccttatccac
     5341 atcgtattca cgcaagagga gcttgattaa ggacgttttc ccagagcctg tctgcccaac
     5401 caagcccagt gtttgccctt ttgccaaact aaagtgaata tccgtcagtg tttcctcatt
     5461 ttcaaaagca aagctgtcaa tggcatactc caaacgccca ttttcaatac catccagagg
     5521 aaactcaggg tcttgtacag gagattcctg agacaaaaga ttttcaatcc gctggtaaga
     5581 aaccttccct cgctgagtag tattaaagag gaagccgatg gccataagag gccagaccag
     5641 catatccaaa tagctgataa aggtgactag attaccaact gtaatctgcc cttcctgaac
     5701 catcaaggag ccaaccaaaa gcgttaaaac ataggacgaa ccaacaaaca agagaaccat
     5761 agggtcaaag agactatcat atttcatggt ttgcaggttc ttttggaagg ttaattcatt
     5821 gactgcctga aaagacttca actcgtctgc ctgataaccg aaagacttgg tcactttgat
     5881 acctgatacg gactcctgta ccttgttatt gagttcagaa aaagcagctt gggattcgcc
     5941 aaaggcctta tgagtctttc tccctaggcg actagtcgca taggccatga aaggtagggg
     6001 gagaatggca acaagagtca tctgccatga gatgctaaag agcatggtca acaaagtcac
     6061 cagagccgtg atagaggcat ccaccgcaga catgacaccg ccacctgcta aacgagtcaa
     6121 ggcattgata tcattggttg cgtgtgccat cagatcaccc gtccgatagg tttgataaaa
     6181 ggctggcgac atttttgtga aatgcttaaa caagcgagac cgcatgatct gtcccaagca
     6241 ataagaggtc ccaaggatat acatacgcca cacatagcgc aaatagtaca taccaaaggc
     6301 tgcaagtagc aagtaaaata ggctaagaag gaggtcctgc tgggttaatt gccccgatgt
     6361 gatggcatca atgactcgcc ccataaccat aggaggaatg agattgagaa cggaaaccaa
     6421 gaccagggcc acaatcccga ctagataacg gtgtttttct aacttgaaaa accaccagag
     6481 tttttgaata atggacataa aatccctttc tgattgcaaa tggaaacctg aggc
//
LOCUS       DAVBHS010000113         6502 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVBHS010000113 DAVBHS010000000
VERSION     DAVBHS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.113"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            104..394
                     /locus_tag="VQ651_001609"
     CDS             104..394
                     /locus_tag="VQ651_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754578.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKNAKNCVRLSQLSLNKR"
     gene            418..1134
                     /locus_tag="VQ651_001610"
     CDS             418..1134
                     /locus_tag="VQ651_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2754579.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIISDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(1263..2078)
                     /locus_tag="VQ651_001611"
     CDS             complement(1263..2078)
                     /locus_tag="VQ651_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HET2754580.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(2075..2980)
                     /locus_tag="VQ651_001612"
     CDS             complement(2075..2980)
                     /locus_tag="VQ651_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET2754581.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMLFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            3378..5507
                     /locus_tag="VQ651_001613"
     CDS             3378..5507
                     /locus_tag="VQ651_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HET2754582.1"
                     /translation="MDKKYEKISQDLGVMLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRAQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYTAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVSVGD
                     LVTVWVKKIDTEREKVNLSLLAPNETD"
     gene            5494..5943
                     /locus_tag="VQ651_001614"
     CDS             5494..5943
                     /locus_tag="VQ651_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HET2754583.1"
                     /translation="MKLTDYVKQVSLEDFGRAFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            6002..6274
                     /locus_tag="VQ651_001615"
     CDS             6002..6274
                     /locus_tag="VQ651_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET2754584.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
BASE COUNT         2036 a         1186 c         1414 g         1866 t
ORIGIN      
        1 tgctaaaatc ttatcagtga gaagatttct taaagtttat tctttgtagt ttcatctgta
       61 ttagtacata atttaactta gagaaaatag gaggactaat cgaatggaat taaaagattt
      121 tacagaaaaa gaacaggaaa tgattaagaa aaggcttaca atgtctaata ttagtgacaa
      181 agaaactact gagaagattc tggcgctagt cccacaagac ttgattaagc gaatcccgtt
      241 ttttgtcaga aaacatgcta caacacgtac gattaaacgt atttcaattg aataccctga
      301 actctacgct gtagctcaaa caagtggtga gattccagaa aaaaacgcga agaattgcgt
      361 cagattatca caactatctt tgaacaaaag atgaataagc atagtattaa gtagagaatg
      421 aaaaaatatt ttattggcgg tttgggaagc aatgcctatc atagcaagga ttttcttcaa
      481 gaactagatt cgcaggtcta ttttctaaat ccatatgaaa agcatcttcg agatgaaaca
      541 gaattgaaat catggtttaa aaatgagatt gtagaggaag aatctatctg tctgataggt
      601 cattctattg gaggcgattt agctcgttat ttcgcatcgg aatttgaaga agtaaagaaa
      661 ctgattcttt tggatggtgg ctatctagat ttagataaga ttttaccttt ggatacagag
      721 ttagaggaaa ctaaaaatta tatcaaatct caaattattt cggacttaga tgttcttact
      781 tctaaagaaa aatctgaagc aaagcattgg tcagaaaata tggagaaagc tgtaagacag
      841 tcctatcact ggaatgttga gtataataga tatgagttgg ctataaatta tgaaaatata
      901 gaagcgatac tccgcctacg gagaaaaata caagctttta agagagaagt gggagatacc
      961 ttgtttatta gtcctcgcta tcctaatgaa gctacatgga gagaggaagc cctaaaagaa
     1021 ttgccagact attttgatac tattttttta gaaaactttg gccatgagct ttatactcaa
     1081 gcacctaaag aaatcgctag tctaatgaat gagtggctcg cttattttct atgagctact
     1141 tgtattgctg gaaatggtat aagatgaaaa tctacaagtc actaattttc atataacaaa
     1201 aaacgaacag gctctttttc tgttattcag aagagaactt gttcgttttt atcttgattt
     1261 tcttaaaaag ctctatatac atagggattt tctggtttat cgactttggt aaagctgtcg
     1321 atttccaagg ttggaaggtt ttgtttgagt tctttatggt agtgattgac cagttttcct
     1381 ttggctgtta tccaagtgtt gttttcatac tgccgggtat tgcccttggt cagcaatcca
     1441 tagacaccag aatctgcgat acagtggata atgccgaatc ggaacagaaa ttgactattg
     1501 gcatgactgg ggtcgttata gacaaagcct gtaaactgga ttgtcttgcc ctcaaactca
     1561 tctggatagt cgtagatagc ctccatgact tccatatagt tttcattagt gatctgaata
     1621 ctatcttggg ataagtattt atccgccgcc gttcgcattt ccttttcata ggctgatttt
     1681 gaaaaataag aactggtatc tggtttcaaa tattggcttg tcgtcccttc gcttgtctga
     1741 atggctagat ctgttccttc cgataggggg aaatgataac ctttagcaga aacggtctga
     1801 gaatccaagc taacagttgg gaaagttaag ccgatgacaa gtggaatagc cagaagagaa
     1861 atactcgtca tcttggccaa tcggctgttc agatgactgt gggttttgac ttgcttcatc
     1921 cagatataca attgaacaat tgccaagata aaggaaagca ccatggagat ataggccaga
     1981 taggaatagt gcatgttgat gtactggttt agtttgcccg acagatggag gtaaatagtc
     2041 agttcaaaat agccagctaa aactaaaaat cgaatcatag gatgactcca atcaagagag
     2101 aatagactaa gacgacaaga gttacaatcg ttatgaagtg actgataaat cgtgctttca
     2161 agtaattttt catcatgaga atatttttga tatccagcat tggaccaatg acgagaaagg
     2221 ccagaactgg tgccaaaccg aaactcgaga gaagagaagc acctataaag gcatccgcct
     2281 cactacagag cgaaagaaga aaggccaaaa tcatcaagag caggatggca aaaagaggag
     2341 tcgcactgat agaggtcaga atccgagtcg gaacgtagac ctgtattata gaagcaaaga
     2401 ggcagccaaa taccaaataa cgccccgtat caaaaaattc atcaatggcc tgcacaaaga
     2461 cttgaaaaac tttttttgca gaactcaagt aagaaaaatc atgctcatga caagccagac
     2521 gattttcttt ctgaatcggt tcttgccaga aaaatcctag aaaaattcct agtattacag
     2581 ccacaagaat ggaacccaga gctcgtaata gggcgacatg gaaggagttg ccaaaggcag
     2641 aataggtcgc aaaaagaaca atgggattga taacaggtgc tgtcacaaga aaaggaacgg
     2701 ctgtgtaact gggaaccttt ttttccagaa aacgattgat gatggggacg attccacatt
     2761 cacaagaagg gaaaagcata ccgacaaagg tcccaaaaaa gattctcccc caacgatttc
     2821 gagggagaaa atgataaacc ttgtcaggtg tgatataaac ttcaatcagc cctgagacaa
     2881 tgcttcctat tagaacgaag ggaagggctt caatgataat ggagagaaaa atggctccag
     2941 tttgaagtac actagatggg agttgttgaa aagcagtcat ctagtttttc ttttctgctt
     3001 tggctttttt ctctttgtca tcagggaaag taacttgttt caaatcaggg agtttagcaa
     3061 actcttcaaa catcttgtcc aagtcgtctc gttttgtaaa ttttacagcc ataggtgtac
     3121 ctcgattcta aagtctacct ctattatact attattttgc ggaaaaggga gttttttaat
     3181 ttgttttcga tagttgatac acttcgaaaa tctcttcaaa ccacgtcagc ttcgccttac
     3241 cgtatatatg tgacttatcc acaacctcaa aacagtattt tgagcagcct gcggctagct
     3301 tcctagtttg ctttttgatt ttcattgagt atgaaaagga ttggagaaag ccttccagct
     3361 tgtggtataa tagatttatg gataaaaaat atgaaaaaat ctctcaggat ttgggagtga
     3421 tgttaaagca aattgatacc gttctaagtt tgacagctga aggggcgact attcccttta
     3481 tcgcgcgtta tcgcaaggac atgactggta gtctggatga ggtggcgatt aaggctatta
     3541 ttgatttgga taaaagtctg acaaatctca atgaccgtaa ggaagctgtc ttagctaaga
     3601 ttcaagaaca aggtaagttg accaaggaat tggaagaagc tatcttagtt gccgaaaaat
     3661 tagcagacgt tgaagaactc tatcttcctt ataaggaaaa gcgtcgtacc aaggcaacca
     3721 ttgcccgtga agctggactc tttcctcttg ctcgtttgat tttgcagaat atagttgact
     3781 tagagaaaga agctgaaaag ttcgtctgtg aaggatttgc gactggcaag gaagccttga
     3841 ccggtgcagt tgatattttg gtcgaagcct tatcggaaga tgtgaccttg cgttctatga
     3901 cttatcagga agtgctgaga cactctaaac ttacttctca agccaaggat gaaagtcttg
     3961 atgaaaagca ggtttttcag atttattatg atttttcaga gacagttgga actatgcaag
     4021 gctatcgtac cttggctctc aatcgtgggg agaaacttgg tgtcttgaag atcggttttg
     4081 aacatgcgac ggaccgtatt cttgccttct ttgctactcg tttcaaggtg aaaaatgctt
     4141 atattgatga agttgttcag caatccgtta agaaaaaggt cttgcctgct atcgagcgtc
     4201 gtattcggac agaattaact gagaaagctg aagaaggagc tatccaactt ttttctgaca
     4261 atctgcgcaa tctcctcttg gttgctccac tgaaagggcg cgtggttctt ggatttgacc
     4321 cagcctttcg tacaggtgcc aagttagctg tcgtggatgc aacaggaaaa atgctgacaa
     4381 ctcaggttat ttatcctgtt aaaccagcat cagctcgtca aatcgaagaa gccaagaaag
     4441 atttagcaga tttaattggt caatacggtg tagagattat tgccattgga aatggaacgg
     4501 ccagtcgtga aagtgaagct tttgtagcgg aagttctgaa agatttccct gaagtcagct
     4561 atgttatcgt taatgaaagt ggtgcttctg tctattctgc cagcgaactt gctcgtcagg
     4621 agtttccaga cttgaccgtt gaaaaacgct ctgccatttc tatcgcccgt cgtttgcaag
     4681 atcctcttgc ggaattggtc aaaatcgatc ctaagtcaat tggtgtcggt caataccaac
     4741 acgatgtcag tcagaagaaa ctatctgaga gtctggactt tgttgtcgat acagtggtta
     4801 accaagttgg tgtcaatgtc aatacagcta gcccagctct tctttcacac gtagctggac
     4861 tcaacaaaac tatctctgaa aatattgtca aataccgcga ggaagaagga aaaatcactt
     4921 cacgcgccca aatcaagaaa gttcctcgtc tgggagccaa ggcctttgag caggctgctg
     4981 gtttccttcg tatccctgaa agtagcaata tccttgataa tacaggagtt cacccagaga
     5041 attacactgc agttaaggaa ctcttcaaac gtttagatat taaagaccta aacgaagaag
     5101 cccaaagcaa actcaagtcc ctttcagtca aggaaatggc tcaagagcta gacctcggtc
     5161 cagaaaccct taaagatatc attgcagatc ttctcaaacc aggtcgagat ttccgtgatt
     5221 cctttgacgc acctgtgctt cgccaagatg tcctagatat caaagaccta gtggtaggtc
     5281 agaagctaga aggtgttgtg cgtaacgttg ttgacttcgg tgccttcgtt gatatcggga
     5341 ttcacgagga cggcttgatt catatttctc atatgagtcg caagtttatc aaacacccta
     5401 gtcaagtggt gtcagttgga gatttggtga ccgtttgggt caagaaaatt gatactgaac
     5461 gtgaaaaagt taatctgtcg ctcctcgctc caaatgaaac tgactgatta tgtcaagcag
     5521 gtttcactag aagacttcgg gagagctttt atccatcatg tccagtggaa taggcgtcta
     5581 cgttcgacag gtgggcgatt tttccccaaa gatgggcatc tggattttaa tcccaaggtt
     5641 tatcagaaac tgggtatgga ggtttttagg aaaattgtcc gacatgaact ctgtcattat
     5701 cacctttatt tccaagggaa gggctatcaa cacaaggatc gggattttaa ggaactgttg
     5761 aaagcagtgg atggattaag gtttgtacca tccttgccca atagtaactc taaaccactc
     5821 aagctttatc gttgtcaatc ctgccagcaa aggtatcagc gcaagcgtag gattgatacc
     5881 aaacgctatc gctgtggact ttgtcgaggt aaattgcttc tgataaatca gcctgaggac
     5941 tgatgaaagc cgagcctccc cgtgatatac tatgtctaac ctagcagaaa gaggaaatat
     6001 catgaacagt aaattttata aaatgagacg caatcgcatg atttcaggag ttctggcagg
     6061 tctgtcagac aagtggaatt ttgatgttac cctagttcgt ttcttgtttg ccatttttac
     6121 agttgcaaac tttggacttg gcgtgattat ctatatcatc ctagcttcta tcatgcctac
     6181 caaggaagaa atcgaagcag aaatgtacgg aacaggccca cgtaaacgca aagaagccca
     6241 agccattgac gacaatgaag gctggttttg gtgaggttct acagaagatt tggaataagc
     6301 aaaaagcttt aaatcaatag tctaaactgc ttgatttaaa gcttttttac ttttatactc
     6361 aataaaaatc aaagtgcaaa ctaggaagcc agccacaggc tgtaatttag tcggcaatgt
     6421 gaagttgaca tagtttgcac tttgattttt gaatagtatt aataatattt cgccagcttc
     6481 atccacaaac tatagtggat tg
//
LOCUS       DAVBHS010000114         6434 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVBHS010000114 DAVBHS010000000
VERSION     DAVBHS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.114"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            209..409
                     /locus_tag="VQ651_001616"
     CDS             209..409
                     /locus_tag="VQ651_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754585.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            434..850
                     /locus_tag="VQ651_001617"
     CDS             434..850
                     /locus_tag="VQ651_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754586.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1081..1788
                     /gene="ccdA2"
                     /locus_tag="VQ651_001618"
     CDS             1081..1788
                     /gene="ccdA2"
                     /locus_tag="VQ651_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET2754587.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1799..2356
                     /gene="sdbB"
                     /locus_tag="VQ651_001619"
     CDS             1799..2356
                     /gene="sdbB"
                     /locus_tag="VQ651_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HET2754588.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2477..3868
                     /locus_tag="VQ651_001620"
     CDS             2477..3868
                     /locus_tag="VQ651_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET2754589.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPSWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4011..4928
                     /gene="adcAII"
                     /locus_tag="VQ651_001621"
     CDS             4011..4928
                     /gene="adcAII"
                     /locus_tag="VQ651_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HET2754590.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            4936..>6434
                     /locus_tag="VQ651_001622"
     CDS             4936..>6434
                     /locus_tag="VQ651_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2754591.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNHFHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLVFDPAQITYRTVNGVAVPHGDHYHFIPYSQLSPLEEKLA
                     RMIAVKGQNGAVLPGMHYLKPAPKPQVQPSTEKKQTDFAVEQVVRKVGEGYVVEIAGV
                     SHYVFAKDLAKDKIDAIENLLSKKTQETHALVAKKENVAPRDQEFYDKAYNLLTQAHK
                     VLSENKGRTSDFQALDKLAERLNNESSNKVKLVDDLLAFLAPITHPERLG"
BASE COUNT         1975 a         1171 c         1351 g         1937 t
ORIGIN      
        1 tttctcttac caacattaac cacaccatcg gttattatag taaaatggaa taagaacagg
       61 acaaatcgat caggacagta aaatcgaatt ctaacaatgt tttagaagtc tatatgtact
      121 attctagttt caatatacca taactgctcc tagtcaacct gcaagtcctg tcccagctcc
      181 aagctcagct ccgactcaag atcctagagt ggtagcaagc gcttcggcta gctcaacgag
      241 cacacaagct caagagcaag ttgacaagtc tgaacttcgt gccttgagtc aagagttaga
      301 ccaacgcttg aaagctttgg caacagtatc tgatccaaaa atcgatgcaa ccaaggctgt
      361 cctcctagat gctcaaaaag ctccggaaga tagtgccttg acagagtaag gtctgcatac
      421 tgcagtagag tctgtgaagg ctgccttgga ctctctcaaa gatgtgaaag caaatgctag
      481 cgacagcaag cctgcacagg acaagaagga tgcaaaacaa ggaacggaag atagtaagga
      541 ttcagataag atgactgaaa caaactcagt tccggcagga gtgattgtgg tcagtctact
      601 tgccctccta ggcgtgattg ccttctggct gattcgccgt aagaaagagt cagaaatcca
      661 gcaattaagc acggaattga tcaaggttct aggacagcta gatgcagaaa aagcggataa
      721 aaaagtcctt gccaaagccc aaaaccttct ccaagaaacc cttgatttcg tgaaagaaga
      781 aaatggctca gcagagacag aaactaaact agtagaggag cttaaagcaa tccttgacaa
      841 actcaagtaa aacatgattt atgaaatgtc agacctaaaa gacattttcg ttcacaaaag
      901 cagtgacgag taatccttgt cgttgctttt tggctatttt gggggataag atccgccttg
      961 actagcaaaa gaaaagattg gtccaatagt gcccgatacg attttgcgta gcagaaagct
     1021 tacaaataaa attgaatata gtaaaatagg attagaatta ttaagaaagt tggttcttta
     1081 ttggaaacga tagtattttt aatctctgtt tttctagcag gtgttttatc ctttttttct
     1141 ccttgtattt ttcctcttct gccagtctat gctgggattt tattggatga tcaagaaagt
     1201 gcaaaaagct tttctttgtt tgggagaaag gttctctggt caggcttgat tcgaacactt
     1261 tgctttatcg ctggtatctc tctcattttc tttattctag gctttggtgc tggttacttt
     1321 ggtcatattc tctatgcaaa ttggtttcga tatgtcatgg gagctattat tatcattttg
     1381 ggtcttcacc agatggaaat ttttcatttg aagaaattag aagttcaaaa aagttttacc
     1441 tttaaaaaat cagattctaa tcgttattgg tcagcttttt tacttggtat tacctttagc
     1501 tttggttgga caccttgtat tggtccagtt ttaagttctg ttttagcact tgcggcttct
     1561 ggaggcaatg gtgcttggca aggagcgatt tatactctca tttacactct gggcatggcc
     1621 cttcctttct tggtcttggc actagcttca ggtctagtca tgccatattt tagtaaaatc
     1681 aagcgtcata tgatgctact aaagaaaatt ggtggtttcc tcattgtttt aatgggaatt
     1741 ttgttactat taggacaagt aaatgttcta gctggaattt ttgaataaag gaggacaaat
     1801 gaaaaaagta atgtttgctg gcttaagtct cttgtcatta gttgtattga tggcctgtgg
     1861 tgaggaagaa actaaaaaga ctcaagcagc acaacagcca aaacaacaaa cgactgtaca
     1921 acaaattgct gttggaaaag atgctccaga cttcacattg caatccatgg atggcaaaga
     1981 agttaagtta tctgatttta agggtaaaaa ggtttacttg aagttttggg cttcatggtg
     2041 tggtccatgc aagaaaagta tgccagagtt gatggaacta gcggcgaaac cagatcgtga
     2101 tttcgaaatt cttactgtca ttgcaccagg aattcaaggt gaaaaaactg ttgagcaatt
     2161 cccacaatgg ttccaggaac aaggatataa ggatatccca gttctttatg ataccaaagc
     2221 aaccaccttc caagcttatc aaattcgaag cattcctaca gaatatttaa ttgatagcca
     2281 aggaaagatt ggaaagattc aatttggtgc tatcagtaat gcggatgcag aagcagcatt
     2341 taaagaaatg aactagaatc gataaaatct gattacaaga tgtagtcaga tttttttaga
     2401 aaaacatttt ataaatattc acatatctcc tatatttatg gtaaaataga attatcagtt
     2461 tattttggag tcaaagatga atatatttag aacaaagaat gttagtttag ataaaacaga
     2521 gatgcatagg catttgaagt tatgggattt gattttgctg ggtatcggag ccatggtagg
     2581 gacaggcgtc tttacaatca caggtactgc agctgcaaca cttgctggcc cagccctagt
     2641 gatttcaatc gttatttctg ccttgtgtgt gggattatca gccctctttt ttgcagaatt
     2701 tgcctcgcga gtacccgcta caggaggtgc ctatagttac ctctatgcta tcttaggaga
     2761 attcccttcc tggttggctg gttggttaac catgatggag ttcatgacag ccatatcagg
     2821 cgtagcttcg ggttgggcag cttattttaa aggcttgctc tctcaatacg ggatagcctt
     2881 tcctcaggct ttaaatggta cctttaatcc tcaagcaggg acatttgttg atttattgcc
     2941 tattcttgtc ttggtcttgg tgacttcgct tgttttacta aatgccaagg cagccttacg
     3001 ctttaattcg attctagtta ttttgaaatt ttccgcttta gctctctttg ttttagtagg
     3061 aatttggaat attaagtttg ataattggag caattttgct ccttacggtt ttggacaaat
     3121 ctatggagct agcactggta ttatggctgg tgcgtccttg atgttcttcg gttttttggg
     3181 atttgaatcc atctctatgg ctgtagatga agtcaagact cctcaaaaaa atattcctag
     3241 agggattgtc ttatcgcttt ctatcgtaac cattctctat gccttggtga cccttgtttt
     3301 gactggtgtg gttcactata gtcatttaaa tgtcgatgat gccgttgcct ttgcccttcg
     3361 tagtgttggg attagttggg cagccaacta tgtgtcatta gtggctatct tgaccttgat
     3421 tacagtttgt atttcgatga cctatgccct atcgcgtatg atttacagtt tagcacgtga
     3481 cggcttagtg cctgctgcct ttaaggaact gacgaagact agcaagatac caaagaatgc
     3541 tactatcttg acaggtctag cttcagcagt agcagcagga atgttcccac tagccagtat
     3601 tgcagccttt ttaaatattt gtaccttagc ctacttgatc atgctggctt atggtctgat
     3661 tcgcttacgg aaagaaaaag gaatgcccaa agctggggaa tttaaaacac cattggtacc
     3721 tctgttacca attttatcga tcatcatttg tttatccttc atgttacagt ataatatgaa
     3781 cacctggatt gctttcctag ttgcgttgtt agtaggaagt attatttact tcacttatgg
     3841 ctataagcat tctaccatag aagaataaag tgaaagtcta ttgagttgtt gacactcagt
     3901 agatttttat atgtgaaaga aatttcaaat tttttctaaa aatatcttga cagattaaat
     3961 tttcaggagt agaatattta ctagttaatt aaatggttaa ggagttgttc atgaagaaac
     4021 aaaatttatt tttagtcctg ttaagtgtct ttcttttgtg tttgggggct tgtggtcaaa
     4081 aggaaagtca gacaggaaag gggatgaaaa ttgtgaccag tttttatcct atctacgcta
     4141 tggttaagga agtatctggt gacttgaatg atgttcggat gattcagtca agtagtggga
     4201 ttcactcctt tgaaccttcg gcaaatgata tcgcagccat ctatgatgca gatgtctttg
     4261 tttaccattc tcatacactc gaatcttggg caggaagtct ggatccaaat ctaaaaaaat
     4321 ccaaagtgaa ggtcttagag gcttctgagg gaatgacctt ggaacgtgtc cctggactag
     4381 aggatgtgga agcaggggat ggagttgatg aaaaaacgct ctatgaccct cacacatggc
     4441 tagatcctga aaaagctgga gaagaagccc aaattatcgc tgataaactt tcagaggtgg
     4501 atagtgagca taaagagact tatcaaaaaa atgcgcaagc ctttatcaaa aaagctcagg
     4561 aattgactaa gaaattccaa ccaaaatttg aaaaagcgac tcagaaaaca tttgtaacac
     4621 aacatacagc cttttcttat ctagcgaaga gatttgggct taatcaactt ggtattgcag
     4681 gtatctctcc tgaacaagaa ccaagtccac gacaactaac agaaattcag gaatttgtta
     4741 agacctataa ggttaaaacg atttttacag aaagtaacgc ttcttcaaaa gtagctgaaa
     4801 ctcttgtcaa atcaacaggt gtgggtctta aaactctgaa tcctttagag tcagacccac
     4861 aaaatgacaa gacctattta gaaaatcttg aagaaaatat gagtattcta gcagaagaat
     4921 taaagtgagg aaagaatgaa aatcaataaa aaatatctag caggttcagt ggcagtcctt
     4981 gccctaagtg tttgttccta tgaacttggt cgtcaccaag ctggtcaggt taagaaagag
     5041 tctaatcgag tttcttatat agatggtgat caggctggtc aaaaggcaga aaatttgaca
     5101 ccagatgaag tcagtaagag agaggggatc aacgccgaac aaattgttat caagattacg
     5161 gatcaaggtt atgtgacctc tcatggagac cattatcatt actataatgg caaggttcct
     5221 tatgatgcca tcatcagtga agaacttctc atgaaagatc cgaattatca gttgaaggat
     5281 tcagacattg tcaatgaaat caagggtggc tatgtgatta aggtagacgg aaaatactat
     5341 gtttacctta aagatgcagc ccatgcggac aatattcgga caaaagaaga gattaaacgt
     5401 cagaagcagg aacgcagtca taatcacaac tcaagagcag ataatgctgt tgctgcagcc
     5461 agagcccaag gacgttatac aacggatgat gggtatatct tcaatgcatc tgatatcatt
     5521 gaggacacgg gtgatgctta tattgtccct cacggcaatc acttccacta tattcctaag
     5581 agtgatttgt ctgccagtga attagctgct gcccaagcct attggaatgg gaagcaggga
     5641 tctcgtcctt cttcaagttc tagtcataat gcaaatccag ctcaaccaag attgtcagag
     5701 aaccacaatc tgactgtcac tccaacttat catcaaaatc aaggggaaaa catttcaagc
     5761 cttttacgtg aattgtatgc taaaccctta tcagaacgcc atgtagaatc tgatggccta
     5821 gtatttgatc cggcacaaat aacatatcgt acagtcaatg gtgttgctgt accgcacgga
     5881 gaccattatc actttattcc ttattcacaa ctgtcacctt tggaagaaaa attggctcgt
     5941 atgattgctg ttaagggaca aaatggtgca gtcttgccag gaatgcatta tttgaaacca
     6001 gcaccaaaac cgcaagttca accatcaact gagaaaaaac aaactgattt tgcagtagaa
     6061 caggttgtta gaaaagttgg agaaggctat gttgtggaaa ttgctggtgt cagtcactat
     6121 gtttttgcta aagacttggc taaggataag attgatgcta ttgaaaatct cttgtctaag
     6181 aaaactcaag agacacatgc tctagttgct aaaaaagaaa atgtcgctcc tcgtgaccaa
     6241 gaattttatg ataaagctta caatctgtta acccaggctc ataaagtctt gtctgaaaat
     6301 aaaggtcgta cttctgattt ccaagcctta gacaaattag cagaacgctt gaataatgaa
     6361 tcttctaata aagtaaaatt ggtagatgat ttattggcat tcctagcacc aattacccac
     6421 ccagaacgcc ttgg
//
LOCUS       DAVBHS010000115         6401 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVBHS010000115 DAVBHS010000000
VERSION     DAVBHS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.115"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            53..1015
                     /locus_tag="VQ651_001623"
     CDS             53..1015
                     /locus_tag="VQ651_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET2754592.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSSNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVIIFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVEPLRYE"
     gene            1192..2199
                     /gene="gap"
                     /locus_tag="VQ651_001624"
     CDS             1192..2199
                     /gene="gap"
                     /locus_tag="VQ651_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET2754593.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2266..3102)
                     /locus_tag="VQ651_001625"
     CDS             complement(2266..3102)
                     /locus_tag="VQ651_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2754594.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQYLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETYVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3228..5423
                     /locus_tag="VQ651_001626"
     CDS             3228..5423
                     /locus_tag="VQ651_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET2754595.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMASQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVAAVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIAYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5476..5628
                     /gene="rpmG"
                     /locus_tag="VQ651_001627"
     CDS             5476..5628
                     /gene="rpmG"
                     /locus_tag="VQ651_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2754596.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5638..5814
                     /gene="secE"
                     /locus_tag="VQ651_001628"
     CDS             5638..5814
                     /gene="secE"
                     /locus_tag="VQ651_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET2754597.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            5870..>6401
                     /gene="nusG"
                     /locus_tag="VQ651_001629"
     CDS             5870..>6401
                     /gene="nusG"
                     /locus_tag="VQ651_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET2754598.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         1989 a         1117 c         1396 g         1899 t
ORIGIN      
        1 cacggataca gttgggaaag aagctattaa acatttggag agtccttttg ggatgaacat
       61 gtcaaatatt actatagatg ggcaagaaat ggatctgagt caaacgatgc cttcttatgc
      121 tcaagtagtg agtctttatg atgatacaag tatctctgtt agtagtaatg agacggacaa
      181 agtgttggct ggctccctct atactgatac aaatgaacag ggattaacga ttccaagcag
      241 tttactaaaa aattggaatg aacagacagg aaaaaatttg acagctaatg atcttattgg
      301 caaatcagtc tcagccagca ttgtagaaag tgctgccgaa actagtaaga ttgctcaatt
      361 tcaaacgaag attgtacgtg taatcaatga tgaagatgac atggaggaca gcaacagttt
      421 catgctgtct catcaaatgg aaacgatttt gaaagaggct ggatttacga aagctgtatc
      481 ttattttatc ttggaactca aagatccatc acagacaaaa gtagtaacag aagaattaca
      541 gaaaaataag aagtatactg tgctttctca acagagagtt cttgatattg tgattatctt
      601 tattcgtgtt attcagggat tattgattgt gctttcatca caagctattg tggtagcagc
      661 ggttatgatt ggtatcatta tttacatcaa tatcatgcag cgttccaagg aaataggtgt
      721 catgaaggca gttggttatc agaatcgtga tgtcaaagga atttttattt acgaggctat
      781 ctggattgta ggcatcgcct tgctgctggc atttttggtt gcacaagggg tgggaagttt
      841 ggcgaatgcg attgtaagtc acttttaccc atccatcact aaagtttttg aattaaatct
      901 tttatctgtt ttaggaactc tagttttcgc tctattactt ggttatgtct cagcctactt
      961 cccggcgcgt aagattagta aaatggatcc tgtagaaccg ttacgctatg aatagtaagg
     1021 gattccttga tttacagtct taaaaagaaa ctaacataga aaaacaatgg acatacttgc
     1081 aattcaactc aaatagttgt ataataggtt tgttgaaagg tgatttgtag tgattcaagt
     1141 tactcttttc acaataaaaa tttcatgatt ttcataagga ggaaatcact aatggtagtt
     1201 aaagttggta ttaacggttt cggacgtatc ggtcgtcttg ctttccgtcg tatccaaaac
     1261 gtagaaggtg ttgaagttac acgcatcaac gaccttacag atccagttat gcttgcacac
     1321 ttgttgaaat acgacacaac tcaaggtcgt ttcgacggta ctgttgaagt taaagaaggt
     1381 ggatttgaag ttaacggtaa attcatcaaa gtttctgctg aacgtgatcc agaacaaatc
     1441 gactgggcta ctgacggtgt agaaatcgtt cttgaagcta ctggtttctt tgctaagaaa
     1501 gaagcagctg aaaaacacct taaaggtgga gctaaaaaag ttgttatcac tgctcctggt
     1561 ggaaacgacg ttaaaacagt tgtattcaat actaaccacg acgttcttga cggtactgaa
     1621 acagttatct caggtgcttc atgtactaca aactgcttgg ctccaatggc taaagctctt
     1681 caagacaact ttggtgttgt tgaaggattg atgactacta tccacgctta cactggtgac
     1741 caaatgatcc ttgacggacc acaccgtggt ggtgaccttc gccgtgctcg cgctggtgct
     1801 gcaaacatcg ttcctaactc aactggtgct gcaaaagcta tcggtcttgt aatcccagaa
     1861 ttgaatggta aacttgatgg atctgcacaa cgcgttccaa ctccaactgg atcagttact
     1921 gaattggtag cagttcttga aaagaacgtt actgttgatg aagtgaacgc agctatgaaa
     1981 gcagcttcaa acgaatcata cggttacaca gaagatccaa tcgtatcttc agatatcgta
     2041 ggtatgtctt acggttcatt gtttgacgca actcaaacta aagttcttga cgttgacggt
     2101 aaacaattgg ttaaagttgt atcatggtac gacaacgaaa tgtcatacac tgcacaactt
     2161 gttcgtactc ttgaatactt cgcaaaaatt gctaaataat tcttgagttg atagaaagca
     2221 aggctttgtg gtcttgcttt ttatatggaa aaatggatga cacgatcatc cattcttttt
     2281 taattctttt tcaaatgtat ttgaaagggt agtgaaagtt agcttctcta aagtaagtgg
     2341 gtgggtaaag gaaagtcgga aggcatgaag cataagccga cttgtctttg atttactatt
     2401 atagagaggg tcgcccagga taggaagatt atgatgcgaa aggtgcacac gaatctgatg
     2461 ggttcgccct gtctttagct tgcaacgagc caaggaagtc ttgtttgaga attgctttaa
     2521 tctgcttaca tacgtttcag catattgccc attttttgca tcaactattc tttttctacg
     2581 atcatggcga tcacgtccaa ttttgtctct gaaaacaagt tcttttctgt tgatatttcc
     2641 atcaactaga gcccaatatt ctctagaaat ctcttttttc tccaataagc gattgagaat
     2701 gggcaggata aaaggatttt tggcaaagag aactaagcca ctggtttcca tgtccagacg
     2761 atgaacgaca tagcaggttt ggccaacata ggtactgaca tggttaagaa gggcaatttc
     2821 gtttggttga ttaccatgcg ttttcatccc ctctggtttg tttacaataa tcaagtattg
     2881 atcttgataa acttcctgca ctaagtctgg gttgccccaa gggatcgtct tttggggata
     2941 atcttcctcg tcaaaagtca actggcaaac atctccagga tttacgattt cgttccagtg
     3001 gacttcttct tgatttatca aaatatgttt cttgattctc aaaaaatgac ggatttttct
     3061 agggatgagg agttgttcct caagtaattg ctttaccgtc atttgaggta gagaggcggg
     3121 taatgtaaat gtgaattgca tacagatatt gtaacaaaaa aagccctatt tggataggaa
     3181 atagctaaat tcttgtcttc ctatgatgaa gatgataaaa taaacgcatg aaattagata
     3241 aattatttga gaaatttctt tctcttttta aaaaagaaac aagtgaacta gaggactctg
     3301 attctactat cttacgtcgc tctcgtagtg atcgaaaaaa attagcccaa gtaggtccga
     3361 ttcgaaaatt ctggcgtcgt tatcatctaa caaagattat ccttatacta ggtttgagtg
     3421 caggcttgct agttggaatc tatttgtttg ctgtagccaa gtcgaccaat gtcaatgatt
     3481 tgcaaaatgc cttgaaaact cggactctta tttttgaccg tgaagaaaaa gaggctggtg
     3541 ccttgtctgg tcaaaaggga acctatgttg agctgactga tatcagtaaa aacttgcaga
     3601 atgctgttat tgcgacagaa gaccgttctt tctataaaaa tgacgggatt aactatggcc
     3661 gtttcttctt ggctattgtc actgctggac gttcaggtgg tggctctacc attacccaac
     3721 agctggctaa aaacgcctat ttatcacagg atcaaactgt tgagagaaaa gcgaaagaat
     3781 ttttccttgc cttagaatta agcaaaaaat atagtaagga gcaaattcta accatgtacc
     3841 ttaacaacgc ttattttgga aatggtgtgt ggggtgtaga agatgcgagt aagaaatact
     3901 ttggagtttc tgcatcagaa gtgagtctgg atcaagctgc gactctggca gggatgctca
     3961 aggggccgga actgtataat cccttgaatt ccgtagaaga ttctactaat cggcgcgata
     4021 ctgtcttgca gaatatggtt gcagcaggat atattgataa aaaccaagaa accgaagctg
     4081 ctgaagttga tatggcttcg caattgcacg ataagtatga aggaaaaatc tcagattacc
     4141 gttacccctc ttattttgat gcggtggtta atgaagctgt ttccaagtat aatctaacag
     4201 aggaagagat tgtcaataat ggctaccgca tttacacaga gctggaccaa aactaccaag
     4261 caaatatgca gattgtttat gaaaacacat cgctatttcc gagggcagag gatggaacgt
     4321 ttgctcaatc aggaagtgta gctctcgaac cgaaaacagg gggagttcgt ggagttgtcg
     4381 gtcaagttgc tgacaatgat aaaactggat tccggaattt caactatgca acccaatcaa
     4441 agcgtagtcc tggttctaca attaagcctt tagttgttta tacgccagca gttgaagcag
     4501 gctgggcttt gaataagcag ttggataacc ataccatgca gtatgacagc tataaggttg
     4561 ataactatgc agggatcaaa acgagtcgag aagttcctat gtatcaagcc ttggcagaat
     4621 cgcttaatct acctgctgtt gccgctgtta atgatttggg tgtcgacaag gcttttgagg
     4681 caggcgaaaa attcggactc aacatggaaa aggtcgaccg tgttcttggt gtcgccttgg
     4741 gaagcggtgt tgaaaccaac cctcttcaaa tggctcaagc atacgctgcc tttgcaaatg
     4801 aaggtttaat gcctgaagct cattttatta gtagaattga aaatgctagt ggacaagtta
     4861 ttgcgagtca taaaaattca caaaaacggg tgattgataa gtctgtagct gacaagatga
     4921 ccagtatgat gttggggact ttcaccaacg gtaccggtat tagttcatcg cctgcagact
     4981 atattatggc agggaaaact ggaacaactg aagcagtttt caatccggag tacacaagtg
     5041 accagtgggt aattgcttat actccggatg tagtgattag ccactggctt ggctttccta
     5101 ccactgatga aaatcactat ctagctggct ctacttcaaa cggtgcagct catgtcttta
     5161 gaaacattgc caataccatt ttaccttata cgccaggaag tacctttacg gttgaaaatg
     5221 cttataagca aaatggaatt gcaccagcca atacaaaaag acaagtacaa accaatgata
     5281 atagccagac agatgataat ttgtctgata ttcgagggcg tgcgcaaagt ctagtagatg
     5341 aggctagccg ggctatctca gatgcgaaga ttaaggaaaa ggctcaaaca atatgggatt
     5401 cgatagtcaa tctatttcgc taagatgctt gtcaaagcct agctttcttg ttataatgga
     5461 taggatggag gcgttatggc actaaaaaaa gcaagcctag cttgtgcggt ttgtggttcg
     5521 agaaactatt caatcaagat cagcggaaac cccaagccta cacgactaga agtaaataaa
     5581 ttttgtaagc attgtggcaa gtacactaca cacagagaaa cgagatagga gagagcgatg
     5641 cgttttattg gagatatttt tagacttctt aaagacacaa cgtggccaac tcgcaaggaa
     5701 agctggagag attttcgttc tatcatggaa tacacagctt tctttgtagt aattatttac
     5761 atttttgacc agttgattgt ttcaggtttg attcgattta ttaacatttt ttagaagatt
     5821 agtggagtta attacactag aaatcttcta tattatgaaa ggaaatatca tggatagttt
     5881 tgataaaggg tggtttgttt tacaaactta ttctggttat gaaaataagg tgaaagaaaa
     5941 tctattacaa cgtgcacaaa cctacaatat gttggataat attctacgcg ttgaaattcc
     6001 aacacaaaca gtgcaagttg aaaaaaatgg aaagagaaaa gaagtagaag aaaatcgctt
     6061 tccaggttat gttcttgtag aaatggtcat gacagatgaa gcttggtttg ttgttcgaaa
     6121 cacaccaaat gttacaggat ttgtcggatc acacgggaac agatcaaaac caactccatt
     6181 attggaacaa gaaattcgtg acattttggt atctatggga caaactgttc aagaatttga
     6241 tttcgatgtt gagattggtc aaactgtacg tattattgat ggtgcttttg cagactacac
     6301 tggtaagatt acagaaattg ataataacaa agtgaaaatg attatctcta tgtttggtaa
     6361 tgacacagtt gcagaagtaa acctaaacca aatcgcagaa t
//
LOCUS       DAVBHS010000116         6361 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVBHS010000116 DAVBHS010000000
VERSION     DAVBHS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.116"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            136..2316
                     /locus_tag="VQ651_001630"
     CDS             136..2316
                     /locus_tag="VQ651_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2754599.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDTSQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKNLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            2368..3183
                     /locus_tag="VQ651_001631"
     CDS             2368..3183
                     /locus_tag="VQ651_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET2754600.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETLVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            3323..4666
                     /locus_tag="VQ651_001632"
     CDS             3323..4666
                     /locus_tag="VQ651_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2754601.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLEIVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            5043..6233
                     /locus_tag="VQ651_001633"
     CDS             5043..6233
                     /locus_tag="VQ651_001633"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET2754602.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
BASE COUNT         1908 a         1162 c         1404 g         1887 t
ORIGIN      
        1 tagaaatttt ttagaaaaaa gtgtaatttt tttataaaag cctttacaaa aaaaattaaa
       61 gtggtataat taaatcatag aaggtgcaaa cgttttcgta aaacgtttgc ctaaataaaa
      121 ataaaggaga tttgaatgat gaaagataca ttcaaaaatg tcttgtcttt cgaattttgg
      181 caaaaattcg gtaaggcttt gatggtagtt atcgcggtta tgccggctgc tggtttgatg
      241 atttcaatcg gtaagtctat cgtgatgatt aacccaacct ttacaccact tgtcatcaca
      301 ggtggaattc ttgagcaaat cggttggggg gttatcggta accttcacat tttgtttgcc
      361 ctagccattg gaggaagctg ggctaaagaa cgtgctggtg gtgctttcgc cgctggtctt
      421 gccttcatct tgattaaccg tatcactggt acaatttttg gtgtatcagg cgatatgttg
      481 aaaaatccag atgctatggt aactactttc tttggtggtt caatcaaagt tgctgattac
      541 tttatcagtg ttcttgaagc tccagccttg aacatggggg tattcgtagg gattatctca
      601 ggttttgtag gggcaactgc ttacaacaaa tactacaact tccgtaaact tcctgatgca
      661 ctttcattct tcaacgggaa acgtttcgta ccatttgtag ttattcttcg ttcagcaatc
      721 gctgcaattc tacttgctgc tttctggcca gtagttcaaa caggtatcaa taacttcggt
      781 atctggattg ccaactcaca agaaactgct ccaattcttg caccattctt gtatggtact
      841 ttggaacgtt tgctcttgcc atttggtctt caccacatgt tgactatccc aatgaactac
      901 acagctcttg gtggtactta tgacatttta actggtgcag ctaaaggtac tcaagtattc
      961 ggtcaagacc cactatggct tgcatgggta acagaccttg taaaccttaa aggtactgat
     1021 actagtcaat atcaacactt gttagataca gtacatccag ctcgtttcaa agttggacaa
     1081 atgatcggtt cattcggtat cttgatgggt gtgattgttg ctatctaccg taatgttgat
     1141 gctgacaaga aacataaata caaaggtatg atgattgcaa cagctcttgc aacattcttg
     1201 acaggggtta ctgaaccaat cgaatacatg ttcatgttca tcgcaacacc tatgtatctt
     1261 gtttactcac ttgttcaagg tgctgccttc gctatggctg acgtcgtaaa cctacgtatg
     1321 cactcattcg gttcaatcga gttcttgact cgtacaccta ttgcaatcag tgctggtatt
     1381 ggtatggata tcgttaactt cgtttgggta actgttctct ttgctgtaat catgtacttt
     1441 atcgcaaact tcatgattca aaaattcaac tacgcaactc cagggcgcaa cggaaactac
     1501 gaaactgctg aaggttcaga agaaaccagc agcgaagtga aagttgcagc aggctctcaa
     1561 gctgtaaaca ttatcaacct tcttggtgga cgtgtaaaca tcgttgatgt tgatgcatgt
     1621 atgactcgtc ttcgtgtaac tgttaaagat gcagataaag taggaaatgc agagcaatgg
     1681 aaagcagaag gagctatggg tcttgtgatg aaaggacaag gggttcaagc tatctacggt
     1741 ccaaaagctg acattttgaa atctgatatc caagatatcc ttgattcagg tgaaatcatt
     1801 cctgaaactc ttccaagcca aatgactgaa gcacaacaaa acactgttca cttcaaaaat
     1861 cttactgagg aagtttactc agtagcagac ggtcaagttg ttggtttgga acaagtaaag
     1921 gatccagtat ttgctcaaaa aatgatgggt gatggatttg cagtagaacc tgcaaatgga
     1981 aacattgtat ctccagtttc aggtactgtg tcaagcatct tcccaacaaa acatgctttt
     2041 ggtattgtga cggaagcagg tcttgaagta ttggttcaca ttggtttgga cacagtaagt
     2101 cttgaaggta aaccatttac agttcatgtt gctgaaggac aaaaagttgc agcaggagat
     2161 ctccttgtca cagctgactt ggatgctatc cgtgcagcag gacgtgaaac ttcaacagta
     2221 gttgtcttca caaatggtga tgcaattaaa tcagttaaat tagaaaaaac aggttctctt
     2281 gcagctaaaa cagcagttgc taaagtagaa ttgtaatata cttgaggttg gaagctgtat
     2341 tccaacctct tattttggga gaaaagaatg aaatttttaa cactcaatac tcacagttgg
     2401 atggagaaag aagcagagga aaaattccag attttgcttg aagatattct tgaaaaggac
     2461 tatgatttga tttgtttcca agaaatcaat caggagatga cctcgtcaga ggtggaggtt
     2521 aatgaccttt atcaagcttt gccagcagct gagcctattc accaagacca ttatgttaga
     2581 ctcttggttg aaaagttgtt agagcaaggg aaaaattact actggacctg ggcttataac
     2641 catatcggct atgaccgcta ccacgaaggt gtggctatct tgtctaaaac acctattgaa
     2701 gccagagaaa ttttggtttc agatgtggat gatccaacag actatcatac tcgccgtgtt
     2761 gccctagctg aaactttagt cgatggcaag gagctagcag ttgccagtgt tcatctctct
     2821 tggtgggata aaggtttcca agaagaatgg gcacgatttg aggctgtctt gaaaaaattg
     2881 aacaagccac ttttactagc tggagatttt aacaacccag caggtcagga aggttaccaa
     2941 gctattttag ctagtccatt aggcttacaa gacgcatttg aagttgctca agagaaaagt
     3001 ggtagctata ctgttccgcc tgaaattgat ggctggaaag ggaacactga accccttcga
     3061 atcgattatg tctttactac caaagagtta gcggtggaaa atttacatgt cgtatttgat
     3121 ggtaacaaga gtccacaagt gagtgatcac tatggcttga atgctatgtt aaactggaaa
     3181 taataactga aaagaggttg gaactataaa attccagcct tttcttacta gagaagctac
     3241 tggaaatagc ctaaataagt gagactactg taatggaata aaatatggta taattgataa
     3301 gatagataga atcgaggatg ttatgtcatt tacgaaattt caatttaaaa actatattag
     3361 agaagccttg aaggagttaa aatttacaac tccaacagag gtgcaagata agttgattcc
     3421 tattgttttg gcaggtcgtg acctagtagg agaatcaaaa acaggttcag gtaagactca
     3481 tactttcttg ttaccgattt tccagcaatt agatgaagct agcgatagtg tacaagcagt
     3541 gattactgca ccgagtcgtg agttggctac tcaaatttac caagtagcgc gtcagatttc
     3601 agctcactca gatgtcgaag ttcgtgtggt taattatgtg ggtggtacgg ataaggctcg
     3661 ccagattgag aaattggcaa gcaatcagcc tcatattgtt attggaacac caggccgtat
     3721 ctacgacttg gttaaatctg gtgatttagc tattcataaa gccaagacat ttgttgttga
     3781 tgaagcagat atgaccttgg atatgggatt cttggaaatt gttgataaga ttgctggcag
     3841 tcttccaaaa gacttgcaat tcatggtctt ctcagcgact atcccacaaa aactgcaacc
     3901 attcttgaaa aaatacttat caaatcctgt tatggagaaa attaagacta aaacggttat
     3961 ttctgacacc attgataatt ggttgatttc gaccaaggga catgataaga atgctcaaat
     4021 ttaccaattg actcagttga tgcagccgta tttggcaatg atttttgtta acactaaaac
     4081 gcgtgctgat gaattgcatt catatctgac tgctcaaggc ttgaaggttg caaaaatcca
     4141 tggagatatt gcccctcgtg aacgcaagcg aatcatgaat caggtgcaaa atctggattt
     4201 tgagtatatt gtcgcaacag atttagcagc gcgtgggatt gacattgaag gtgtcagcca
     4261 tgtcatcaat gatgccattc cgcaagactt atcttttttt gttcaccgtg ttggtcgtac
     4321 tggacgaaat ggcctaccag gtacagctat taccctttat cagccaagtg atgactcgga
     4381 tatccgtgag ttggagaaat tgggaatcaa gtttagtcct aagatggtca aagatgggga
     4441 atttcaagat acctatgacc gtgatcgtcg tgccaaccgt gagaaaaaac aagataaact
     4501 tgatatcgaa atgattggtt tggttaaaaa gaaaaagaaa aaagtcaaac cgggttataa
     4561 gaagaaaatt caatgggcgg ttgatgaaaa gcgccgtaaa actaagcgtg ctgaaaatcg
     4621 cgctcgcggt cgtgcagagc gtaaagctaa acgccaaaca ttttaataga aattgttgga
     4681 gtactgagct ccaacttttt tatttatgag aacgaactat ctaaaccgaa acactacatt
     4741 aaagactgca aattgcgatt aaaaatggta taatgataaa gttatatagt cccgataaga
     4801 tggtaggtat ttattacgaa gagttttcct atcagtagtt tgcaattcgc aaagaatgac
     4861 ggcttcgagg tcgctcgtct ttctacggtt gccgccatac tgcggtcacc ctatgcgcct
     4921 tactagcttc agaaataaaa aagtatcgtt tttatttctg aagctagtaa ctcttagata
     4981 atggaaattc ctatcagtac tttgtaactc tataacaata ttttttaagg ggggacattt
     5041 ttatgtcaga acgtaaatta ttcacgtctg aatctgtatc tgaggggcat ccggataaga
     5101 ttgcagacca aatttcagat gcgattttgg atgctatttt agcaaaggat ccagaggcgc
     5161 acgttgctgc tgaaacagct gtatatactg gttctgtcca cgtttttggt gaaatttcta
     5221 caaatgccta tgtggatatt aaccgtgtgg ttcgtgatac cattgcagag attggttata
     5281 ccaatacaga atatggattt tctgctgaga cggtgggagt acacccatct ttggtggaac
     5341 aatctcctga catcgctcaa ggtgttaacg aagccttgga ggttcgtgga aatgctgatc
     5401 aagatccact ggacttgatt ggagcaggtg accaagggct catgtttgga tttgcagtag
     5461 atgaaacaga agagctcatg ccattgccaa ttgcactcag tcataaattg gttcgtcgtc
     5521 tggcagaact tcgtaagtct ggagaaatta gctatctccg tccagatgca aaatcacaag
     5581 ttacagttga gtacgatgaa aatgaccgtc cggtacgtgt agatacagtc gttatttcta
     5641 ctcagcatga tccagaggcc actaatgaac aaatccatca agatgtgatt gacaaggtca
     5701 tcaaagaagt tattccatct tcttatcttg atgataagac aaaattcttt atcaatccga
     5761 caggtcgttt tgtaatcggt ggtcctcaag gggactcagg tttgactggt cgtaagatta
     5821 ttgtagatac ttatggtggc tactctcgtc atggtggtgg tgccttctct ggtaaagatg
     5881 cgactaaggt ggatcgttca gcctcttatg cggctcgcta tattgccaag aatatcgttg
     5941 cagcaggcct tgctaagaag gcagaagtgc agttggccta tgctatcggt gttgcgcaac
     6001 ctgtttctgt tcgtatcgat actttcggta caggaacagt agctgaaagt caacttgaaa
     6061 aagcggctcg tcaaatcttt gaccttcgcc ctgcagggat tatccaaatg ctggacctca
     6121 agcgtccaat ttaccgtcaa acatcggctt acggtcacat gggacgtaca gatattgatc
     6181 ttccatggga acgtttggat aaggtagatg ctttgaaaga agcagtaaaa taagatttta
     6241 agaggggaac gtcctctctt ttttatagtt tttaactata ctgggatact gttctgaaaa
     6301 tccattttgc gaaagtagag atttacatgt ataatggatt gaaactagaa tagtacacct
     6361 c
//
LOCUS       DAVBHS010000117         6171 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVBHS010000117 DAVBHS010000000
VERSION     DAVBHS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.117"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..454)
                     /locus_tag="VQ651_001634"
     CDS             complement(<1..454)
                     /locus_tag="VQ651_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2754603.1"
                     /translation="MTIEPGQRIALVGQSGSGKSTLSKILSGLYKIDTGKILFDGVNI
                     NQIDKKILSQNLGVVPQDSFLLNRSILDNITLKNEVTSQKIEEVCKAVQIYDEIMAMP
                     MKFNTIISEMGSNISGGQRQRIALARALINNPSIVILDEATSALDTINE"
     gene            complement(623..1936)
                     /locus_tag="VQ651_001635"
     CDS             complement(623..1936)
                     /locus_tag="VQ651_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2754604.1"
                     /translation="MTRKVPNIEQMSQIECGLCCCLSILHFYKSKETLLDLRRDIEKG
                     RDGYSIGDLKQLLNKRNFDTGSYQVKDVNKISELPLPLIAFWDNQHYVVIYKVKKNKV
                     YIMDPSKGYINYEFKEFSKHFSNIVLLSFPNENYQSLKSQFPSPWIRVFSSFSKVKGR
                     LILTLLFSIISYLIILSVPVMTSKFINSALGNTFSFQTSFLILFSLLCLYLISILARS
                     MGILFSNIFFSRDIESFTFKHLLKLPYSFFELRAKGDILYRISSLSGFRELFTNQVVG
                     GVVDIGTILSVVIYMFLSSKTLSIIALILSLINFLFLFSTRKIMYDTVNRELQEQSLI
                     YSVETEALNTISSIKISGLEDEIYENWSKYLKNVLTKYKKRSIVHILYNSATNVFQLF
                     APIIILIFGLDNVLNGKILLGEVVAFQTMASILFSSEISIFNAYT"
     gene            complement(1929..2477)
                     /locus_tag="VQ651_001636"
                     /pseudo
     CDS             complement(1929..2477)
                     /locus_tag="VQ651_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059560.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2558..2620)
                     /locus_tag="VQ651_001637"
     CDS             complement(2558..2620)
                     /locus_tag="VQ651_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HET2754605.1"
                     /translation="MKKISKFLPILVLVMDIIII"
     gene            complement(2789..3777)
                     /locus_tag="VQ651_001638"
                     /pseudo
     CDS             complement(2789..3777)
                     /locus_tag="VQ651_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050775.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3726..4853)
                     /locus_tag="VQ651_001639"
                     /pseudo
     CDS             complement(3726..4853)
                     /locus_tag="VQ651_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423473.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
     gene            complement(5016..5900)
                     /locus_tag="VQ651_001640"
     CDS             complement(5016..5900)
                     /locus_tag="VQ651_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HET2754606.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            complement(6012..>6171)
                     /locus_tag="VQ651_001641"
     CDS             complement(6012..>6171)
                     /locus_tag="VQ651_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET2754607.1"
                     /translation="LGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
BASE COUNT         2021 a         1153 c          832 g         2165 t
ORIGIN      
        1 cctcattaat agtgtctaat gcactagttg cttcatctaa aattacaata ctaggattat
       61 ttattaatgc acgtgccagt gctatccgtt gcctttgccc acctgaaata tttgacccca
      121 tctctgagat gatagtatta aatttcatcg gcatagccat gatttcatca tagatttgaa
      181 ctgctttaca aacttcctct atcttttgtg aagtaacttc gttctttaaa gttatattat
      241 caagaatact tcggttcaat aaaaatgaat cctgtggaac tactcctaaa ttttgactta
      301 atattttttt atctatttga ttaatattta caccatcaaa taaaattttt cctgtatcaa
      361 tcttatataa tcctgatagt atttttgata acgtactttt tcctgacccc gattgaccga
      421 ctagtgcaat tctctgtcct ggctcaatag tcaaatttaa attttctatt acaggagctg
      481 agtctttgga ataagagaaa gataggtcct taatatctat tctaccttct aaagaacact
      541 ttttaagacc attttcaaca ttctcttcat tttcaagcca gatatcattt actctattta
      601 agtatcctgc tgccaaaata tactacgtat aagcattaaa tattgagatt tctgaactaa
      661 ataaaattga tgccatcgtt tgaaatgcga caacctcacc caataaaatt tttccattta
      721 aaacattatc aagtccaaaa atcaatataa ttataggagc gaacaattga aatacattag
      781 tagctgaatt gtataaaata tgcacaatac ttcgtttttt atactttgtc aatacgtttt
      841 ttaaatactt agaccagttt tcatatattt catcttctaa tcccgatatt tttattgaag
      901 aaatagtatt taatgcttct gtttctactg agtaaatcaa tgattgttct tgtaattctc
      961 tattaaccgt atcatacatt atttttctag tactaaacag aaacaaaaaa ttgattaaac
     1021 tcaatataag agctataatt gataatgttt ttgaactaag aaacatatat attacaacac
     1081 ttagaatagt cccgatatct actactcccc caacaacttg gttagtaaat agctccctaa
     1141 aaccactaag actagaaatt ctgtataaaa tatctccttt tgccctgagt tcaaaaaaac
     1201 tatatggtag tttaagcaaa tgcttaaatg taaaactttc aatatctcta ctaaaaaata
     1261 tattagaaaa tagaattccc atactcctag ccaagataga aataaggtac aaacaaagta
     1321 gagaaaataa aattaaaaac gaggtttgaa aagaaaatgt attccctaat gcagaattaa
     1381 taaactttga agtcattact ggtacggaaa gtattattaa gtaacttatt attgaaaata
     1441 gtaacgtcaa aatcaatcga ccttttacct ttgaaaaact agagaatact cttatccatg
     1501 gagaaggaaa ctgcgacttc aaagattgat aattttcgtt aggaaatgat agcagtacta
     1561 tattactaaa atgttttgaa aactccttaa actcataatt tatatatcct ttagaaggat
     1621 ccattatata gaccttattc ttttttactt tgtaaataac tacataatgt tgattatccc
     1681 agaatgctat taatggtaac ggaagttcac taattttatt tacatcttta acttgatatg
     1741 agccagtatc aaaatttcgt ttattcaata actgtttcaa gtcacctata ctataaccat
     1801 ctctcccttt ttctatatca cgtcttaaat ctagtaatgt ctctttgctt ttatagaaat
     1861 gtagtatgga caagcagcaa cataaaccac attctatttg agacatttgc tcaatattag
     1921 gtacttttct agtcatcatg tcctcttaac tccagtaata aaacattaag taatctatca
     1981 tcttgttttc gagatatttc atagacaact ccagatactc ctagcataaa agaaaatgta
     2041 ttcatagtat tcaaatccgt acttaattct ggtttgataa cctgagaaac gacattatca
     2101 aaataaaaat ctatagattc ttttattaat aggttttctt catctttcaa tattgcatca
     2161 agacaactga tagtacctac agaaccgtga cataaattca attctcttat tttttctaca
     2221 ttagttaata cattttcaat tattgataaa tcattattcc caatattagt tttatcgaaa
     2281 atttcttttc tagctaataa aatgcccgga gctccataac accaaaaatt aggaaaggaa
     2341 tcattatgat ttcttcatat gtctttccaa ttttttaata tattattgaa acatttatct
     2401 tctttatata gtaattcctc tatacatttt aatatatctg tactaggatt aatttcataa
     2461 taatatgata attacattgc tactccagaa taatcgtgtg ctaaacctgt tactgttata
     2521 ttatctaatt gtgaaataca ataattaata cactttttta aatgatgata atatccatca
     2581 ccaaaaccaa aatcggtaaa aattttgaaa ttttcttcat acttggactt cctttccgtt
     2641 tttatttatt gtacaataaa taaaaacgga tttaattttt gttgggaaaa tgggaaatta
     2701 tgaaatcaaa acttggcatc acacttagaa aagttcgtaa aggaaaacaa attagtctat
     2761 gctctgtagc agatgaacat ctttcaaaat cacaaatatc tcgttttgaa cgtggggagt
     2821 cagagatatc ttgtattcga ctaatcaata ttttagacaa attacatatt actttgtagg
     2881 ggagtttttg gctatcgttg ctcaattgtc tgattaaggt cctacccttg atgaaacaat
     2941 tattatccat gttttcttta ttatagacaa agtaagaaga cgtttctcga atgtagactt
     3001 tatatttttt atgattttct tcttccataa tatccaattg atagttggga atgaaaataa
     3061 gaccgctctg tttgacaccg aaagacacct tgatatagac gcccttatca actagcttct
     3121 ctatttggtt ctctgcaagt tccacttcaa attcacgaac ggtatctcat ttttccttaa
     3181 atgtcttaaa ggcttcctca atctcttcag tggatacttt atccttatct cgttcttctt
     3241 ggaaagcatg gtactgttcc tgtaaattct ctaatccttc tgaagcaacg acttccttat
     3301 ttttaaaata atcttgaaaa aatttgacat catataattt cttatcactt attttttgat
     3361 gacccaaact tatcttttga ttattttctt ccaggataaa agttacattt tttgttttaa
     3421 gtcaatggtt agattcaatt cttttgcttt tgttattaaa tcttctaaag aattgacacg
     3481 gtttaacaaa aattctaaac gactttcaat ctcttgctta gcaaaatgcg ttctaaaaaa
     3541 ttcttcatca tatagatctc gtttgctgag ttggcgccct cgaattggtt ttatcatcgt
     3601 tctatctgtc atcaaaaaac ggctatgctt ttgactaaaa tcaatctgaa catgcaactg
     3661 ctttgctttc tctaaaaaat catcaaacga tttagattgc tgaagcaaaa aataaagacg
     3721 ttgtttcaat tcaaatttat gactagattc cttatatttt ttataatctc gaaaagatca
     3781 ggatttttag ctaaaactgc cttacgaaaa tccttccaga actgatggtc aatctcatta
     3841 gccacatcca aacgccaagc atcgatatta aactcttcaa tccaataagt cgcaaccttt
     3901 aaaagataat tcttgacctc tggattggct gtatttagct taggcatata gtcctcgaaa
     3961 ccaaaaacat gatagggtaa gtctctctta ttaactagct tttcagttgt caatgggaat
     4021 tgttgaatat ggaaccaatc cttataagca gactgttcac catttttgac gacatttttt
     4081 cattgaagag attgcgaacc aatatgatta aataccgcat ccagcatgac tttcatgcca
     4141 cgatgatgcg cttgatccac cagttcccga aaggtctcct tgtctccaaa ataacggtca
     4201 atttcaaagt aatctgtcgt attgtacttg tgattacttg tagattcaaa gatgggacaa
     4261 agatatagtc cagtaatacc caagtcttgc aagtaattca tatgatcaat aatcccctgt
     4321 aaatcaccac caaagaaatc atcgctctta ggtgtgacag atgaatccca gtctaaagtc
     4381 ccttctgggt ttaatagagc attgccattg gcaaatcttt caggaaatat ctgataccat
     4441 accgtatttg aaacccagtc aggaaccttg caggcatcaa tctcatgaag ataaggcaac
     4501 ttaaatccat tcccaattgc atgaagattt tctagagaat tttccacaca ccctttatcg
     4561 ccatacaaaa tattttgacc ttctgtatct ctgagctcaa agagatactg gatacgtgca
     4621 aagtcaactg acacttcaac ctgccaatgg tcaaataagg taccagaagt tatcttgacc
     4681 atttcttttg tatcctgata aaactcttcc ataaagataa aagggtcccc atagtgcaag
     4741 ttgatgcttt caatgtcccc tttcttagtt cgaattcgaa tatggagttt cttatcctta
     4801 taaagatagg catactccga ctcaggccta tggtaaatag cactcaattc catcaatttg
     4861 tctcctaaag tattcattca aattttacgc aaacgttttc aaactcaata aatccagtat
     4921 actatttttg taaactattt gcaaggaatg tccgttgtta aaggaactat cttttaaagt
     4981 atacaaaaag ccttaaaaaa ggctttctct atatactaat cctctttcgg ttttgaaaat
     5041 acttctactc gctgagctag gactttgatt tctacaggta ggttatctga tttatctccg
     5101 tcaacatctg actccaattc actatctgaa gagattttaa tattacgcgc tttgatatac
     5161 tcgatattat catttgcgac aacatcccct tttaataaat caggaatgac ggataatttg
     5221 gagaatatag aggcatcttt cagaatcaaa atgttggcat agccgtcctt gttttcttca
     5281 aagattttct tatcagcgaa gtaatttgtc aaaaggacca aaacatggct agcttcacca
     5341 acataatttc cattttctgt ctcaacctta atgttaaaga cctgatctgt catgacagac
     5401 ttcatggtat ttacagcata ggttaaaata ccgaattttg ttttgtcctc tatttcaaca
     5461 ttgtgaatcg cctcaggcag agaaccgata ctaaagatat aaccaaaata gttgtcattt
     5521 gctttaccga tatcaatctt attggttaaa tcaaaatcca gttcgtcaat tgcgccatcg
     5581 atgtcttgat tgatttccaa aagttttgta atgaggttac ccgtaccgcc tgggataatc
     5641 cctaacttag gaatgtagtc tctctcatca atacctgaaa tgacttcatt gacagttcca
     5701 tctccaccaa acacaaccac tgcatcatac tgctcacgag aagcttcttc agcaaaatgt
     5761 gttgcatcca gcgctttttc ggtaattttg gtttcaacat attcaaagta ttcttttgct
     5821 ttattctcca gcttttcttt gtaatccaaa gccttctcgc caccagaagt agggttgata
     5881 attaccattg cttttttcat tgattttatc cttaatttta aacagaaatg tttacatttc
     5941 gtcgtatgca agtaaatgta atcctattat acaatgaaaa tacagaaaag agaaatctga
     6001 cgtactggag attaatacgc ttttattcta ttttcccatc gcctaactac atcctttaag
     6061 ggttcatcca agtaagaata ggccttatcc ttgatccaat caggaatacc gtaagctgcc
     6121 tctgctatgt tacaagtgat tgctgcgaga gtatcactgt cgccaccaag t
//
LOCUS       DAVBHS010000118         6108 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVBHS010000118 DAVBHS010000000
VERSION     DAVBHS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.118"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(40..672)
                     /locus_tag="VQ651_001642"
     CDS             complement(40..672)
                     /locus_tag="VQ651_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2754608.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(694..1566)
                     /gene="sdaAA"
                     /locus_tag="VQ651_001643"
     CDS             complement(694..1566)
                     /gene="sdaAA"
                     /locus_tag="VQ651_001643"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HET2754609.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(1575..2246)
                     /gene="sdaAB"
                     /locus_tag="VQ651_001644"
     CDS             complement(1575..2246)
                     /gene="sdaAB"
                     /locus_tag="VQ651_001644"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HET2754610.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            2488..3060
                     /locus_tag="VQ651_001645"
     CDS             2488..3060
                     /locus_tag="VQ651_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET2754611.1"
                     /translation="MKSITKKIKATLAGVAALFAVFSPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(3112..3975)
                     /locus_tag="VQ651_001646"
     CDS             complement(3112..3975)
                     /locus_tag="VQ651_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2754612.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVEIYYQAKER"
     gene            4441..4779
                     /locus_tag="VQ651_001647"
     CDS             4441..4779
                     /locus_tag="VQ651_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HET2754613.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            4757..5116
                     /locus_tag="VQ651_001648"
     CDS             4757..5116
                     /locus_tag="VQ651_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754614.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WILPILLMAGMGDVIIN"
     gene            5135..5347
                     /locus_tag="VQ651_001649"
     CDS             5135..5347
                     /locus_tag="VQ651_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754615.1"
                     /translation="MMVVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKKLQIK"
     gene            5762..5830
                     /locus_tag="VQ651_001650"
                     /pseudo
     CDS             5762..5830
                     /locus_tag="VQ651_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
     gene            5810..>6108
                     /locus_tag="VQ651_001651"
     CDS             5810..>6108
                     /locus_tag="VQ651_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET2754616.1"
                     /translation="MMYLDGGGVGCNWWNKRDNIAAAAVSGGMSVYQTYAIKKLIANR
                     GMMSRVVRSAITRYIGGVSMATITNALNIAATVAEVFSLGGAIAYGLDIVDGKFDG"
BASE COUNT         1882 a         1033 c         1298 g         1895 t
ORIGIN      
        1 ttcattttct tctgaaatag agttgtcgcc cagtctatgc tattgaaaat acgccaaaac
       61 ttctaagggt ttgtgagcga tataatcagg ttgatagttt agtagatctg cttgctctcc
      121 aaatccccaa gtgacggcca atttctgaat acctgtttct cgagctccca gcatatcaaa
      181 cttggtatct ccgatgatga tggcttgttc tggtgctagt tgatgtgtct gtaaggcttg
      241 gtgaatgaca tctgccttat ggggtgcttc agggctagaa ccataaatac catcaaagaa
      301 atgatggatt tccaagtttt tggccatgtc ttgagcagta gatgtattct ttgtcgtggt
      361 gatgtagagt ggataactgc tcgataactc ctcaagcaag tctataatct gaggaaagag
      421 ttgagcttca tagatgcctt ttgccttata gtaagaacga tatatctgca tggcttcaga
      481 aatttggtct ttggacaggc aggtcgcaaa actactttcg agaggtggtc ccataaaacc
      541 acgaatagtt ttggcatcag ggctaggcac ccccagctct ttaaaggtat aggtaaaggc
      601 attgtgaatc ccgatagaac tatcaacgag ggttccatcc aaatcgaaaa aaatcgctgt
      661 gatagaggtc atggtttctc ctatttgata agcttattct ccgaaaatct ctttttggag
      721 gcgacgacca gtaggggtgg tagcgagtcc accttcagct gtttcacgaa aggcagttgg
      781 catgcttgct cctacttggt acatggcatc gatcacttca tccacaggga ttttagattc
      841 gatacctgcc aaggccatgt ctgctgcgat gaaagcaaag ctagctccca tggcattacg
      901 tttgacacag ggaacttcga ccaaacctgc aacagggtca cagatgaggc ctagcatatt
      961 tttaatgaca aaggcaatag cttgactggc ctgataaggt gttccacctg cagccagagt
     1021 caaggcggca gcactcatag cagaggctga accaacttca gcttgacacc caccctcagc
     1081 acctgagatg gaggcattgt ttgcgatgac tagtccaaag gcaccagcag caaagaggaa
     1141 atccaattgt tgctcgtggc tgaggtctaa tttttcaata gcagcagtga gaacggaagg
     1201 gagacaaccg gcacttcctg cggtaggagt agcgcagacc aagcccattt tagcattgtg
     1261 ttcattgact gcgatagcat ttcgggcagc agagagaatc gtgtaatctg ataaagtttt
     1321 ttcgtttttg atgtagtgat ccaatttggc agcatctcca cctgtcaggc cactacgaga
     1381 tttattttca ttgaggccaa gttggacaga ggctttcata acttccagat tgcgttccat
     1441 gagaaggaag acttcttcac gttcgcgacc ggtcaattca aactctgttg taatcatgag
     1501 ttctgcgaca tttccttgaa agtccagatc tgcttgctcg accaattctt tgatagaata
     1561 aaacatgctt cctcctattt aaagaaattg acattgtgga gatgagggat ttttcgaatt
     1621 tcttcgatag cctcatcaca gttgcgactg tcaacttcga taatcataat ggctttttca
     1681 ccagcttttt cacgagtgac attcatctgg gcgatattga taccatagcg ggaaagcgcc
     1741 tctgtaacaa gggcaatcat acctggaata tcttgatgaa cgatgatgat agtcggtgta
     1801 ttcatattga gagagacggc aaaaccattg agttcggtta cctgaatatt tcctccaccg
     1861 atagaaatac cagtcacgct gatggtcttg tgggcatttt taacagtaat tttagtggtg
     1921 ttagggtgag gggcattgct gtctttctga atggtccaga caatcttgat accacgcttg
     1981 tgggcaattt ccagactatt tggaatttca ggatcatctg tatccattcc taaaatacct
     2041 gcaacaaggg ctaggtctgt tccgtgacca cgataggtct tggcaaatga gttaaaaagt
     2101 tggaattcaa cttctgtcgg agtatcatca aaaatggaag agacaatctt cccaatacga
     2161 acagcaccag cggtatggct actagatggg ccaatcataa ctggtccgat gatatcaaag
     2221 acagattgaa aacgaagtga tttcatcagt ttccccttat aaaaattctt atctctatta
     2281 tatcaaagaa tgaggggctt ggctttaatt gtggatgaaa acctttctaa tacctcaaat
     2341 agcataaaaa tagtatcttt tatgacaaaa aacaccttat ttagggaaat aaaaaataat
     2401 tttgtaatat ttctacataa aagtgtcaag aaacagtaat atttaaaggg tatgatagaa
     2461 ctatagaaag aaggagaatt ttcgaatatg aaatcaataa ctaaaaagat taaagcaact
     2521 cttgcaggag tagctgcctt gtttgcagta ttttctccat catttgtatc tgctcaagaa
     2581 tcatcaactt acactgttaa agaaggtgat acactttcag aaatcgctga aactcacaac
     2641 acaacagttg aaaaattggc agaaaacaac cacattgata acattcattt gatttatgtt
     2701 gatcaagagt tggttatcga tggccctgta gcgcctgttg caacaccagc gccagctact
     2761 tatgcggcac cagccgctca agatgaaact gtttcagctc cagtagtaag tgaaacagtt
     2821 gtttcaactg taagcggatc tgaagcagaa gccaaagaat ggatcgctca aaaagaatca
     2881 ggtggtagct atacagctac aaatggacgt tatatcggac gttaccaatt aacagattca
     2941 tacctgaacg gtgactactc agctgaaaac caagaacgtg tagcagatgc ctacgttgca
     3001 ggacgttacg gttcatggac tgctgctaaa aacttctggc ttaacaatgg ctggtattaa
     3061 gaactaacga acaaataata tgtaaagagt cgaggaaatc cctcggcttt tttatctttc
     3121 cttcgcttgg taatagatct ctacttcttt tttgagatga gacagcatag aagtttcctc
     3181 ggttagctcc agaagggaga accctttttg gataagttta gaatcgtctg tacaaatccc
     3241 aatacggaca tagcagatag ccagcctatc gtagcttttc ttattcttgg cgtcctccag
     3301 taaagtgtag cagatttgct tacacatgtt tttatcttga ttgtatagat agagtgtgga
     3361 aaggtttaat agaattgtca ttcgcaagtc atataaatgt tgatagtttt tatagacttc
     3421 taggcgttgc aatatttttc cagtgatgag atggaggtat tcaatgggaa aactgaaaag
     3481 aatggtattg aggattttta gatcactctc ataccatgta tcttgttttt caattttttt
     3541 ccaaagtttt ttgacaacct gttctgcgtg ttttgatagt tcccctgccc cgtgttgacg
     3601 gagataaata acaacttcaa gcatatctct gatttcttct atagggagat cgtggtgggt
     3661 cttgaggtag tcttggcatt tttgaaataa attgacaaga tcgctggtcc cgataatgga
     3721 acgcatattg agataggttt gcataatctc tgtgcgttga cttggttgat aaagttgaca
     3781 gatatattca aactcttcaa aactcatatt gatttgacgg agaagaaatt ccatattttc
     3841 atattttggg gttgtcttgc cactttcgat ttttgataag ctggttcttg agagaacacc
     3901 cccacagacc tcttcttggg tcagcccctt tgactcacgt atttctttat agacctttcc
     3961 gaaatcataa cgcatgattt tctcctttct gtgaaaaaag ttaacaaaca ataaaatttt
     4021 gttaaaaata ttgtatcata gcaatgtaaa aagtaaaaaa gaaagtcgaa ataaatttgt
     4081 gaaaaaagtt aacgtaattt taaaatgagc ttaataagag gtatgatgta ggtagaaaat
     4141 aaagatagga gaaaaagtat gaataaaaaa atattttgta ttctagtatg tattatatca
     4201 ttgattagtc tggccataat ctttccatgg ggctggtcga tataatgtaa gggagcttga
     4261 gttgttaaag gaggcttagt ccttggacaa aaatctgctg tactacaatg cttggaaaga
     4321 tatcaagaac aaagaatgat acgattgaaa ataaactgat tcaagaacta ctgggcagtg
     4381 attgaggttt gggtgacatc aaagaaaagt tagattaatt ctaaagaatt gagtatttct
     4441 atgttaaaaa aatatccttg tacgatgcaa catgatcagt cagattgtgc tgcggcagtt
     4501 gtttcaacag ttcttttatc ttacaaaaag gaactatcaa tcatgaagat tcgggaaatt
     4561 attggtacag acatgtatgg aacgactgtc agtggtattg tttcaggtct gaataagttg
     4621 aattttacag taaaagctgt tcgagtagca ctggaagatt tgactccaaa attaacattt
     4681 cctgcgattc ttcaagttaa gaatgattta ggtcaaaatc attttgtggt attacatagt
     4741 ataaaggaga aaataaatgg aactcgtatt accaaataat tatgttgtga ttgatgaaga
     4801 agagatgatg taccttgatg ggggggctat atatataccc aggtgggcaa ttacaggagc
     4861 cattactggt gcagcatatg cagcattagc agcagcagga ggtggaggcc ttcaactagt
     4921 tcttgcatct tatggattac gctccgcact ggtagctggg attgttaaag gtttaggagt
     4981 attaggaatt catattggaa atgcttttgc aaatactgtt attagaagta ttgcatctgc
     5041 tggaattggt gctggagctg attggatttt accaatatta ttgatggctg ggatgggcga
     5101 cgtgataatc aattgagaat aggataatag tattatgatg gttgttttaa tttcagttat
     5161 tgttgcattc ctgtaccata aaattacaga gtatgtcaac aacaggaaaa ttgatattgt
     5221 tatttttcta tcattgctga tttctcattt tatttggaat tactattttt taccaattga
     5281 aatattgata gcaatcagtg gagcaaattt attattaagt tttttaaaaa aattacagat
     5341 taaataatat tattccatat gacatatcaa ctaggtcatt agattttatt gaagatataa
     5401 attaaaaacg aggttgggaa aaaactcaat ttgaaagtga aattgagtaa atttttctat
     5461 tatataacgc atattatcaa gatttttgaa cacctgataa tatgcacttt tcttatttta
     5521 aagatttttt gcctttcttc atttcttgaa tgaattggag agatgacttt atttaggtgt
     5581 ttgacattac tatagatagg atttagcagt actaatatga actcatatta taggtgcaga
     5641 cttaaatttt ggaagcaagc taaaggatat cttatctagg taactaagtg ctataaacaa
     5701 agtcaacgat cacttgttag gaaacgatcc taactatgct taataaaaaa ggagaaaata
     5761 aatggaactc gtattaccaa ataattatgt tgttcttgag caagaagaga tgatgtatct
     5821 tgatgggggt ggtgttggtt gtaactggtg gaacaaacgt gataatattg ctgcagctgc
     5881 agtttctgga gggatgtcag tataccagac atatgccatt aaaaagttaa ttgctaatcg
     5941 tggtatgatg agccgagtcg ttagaagtgc tataacaaga tatattggtg gagttagtat
     6001 ggcaactatt actaatgctc ttaatattgc ggctactgtt gctgaagttt tctcattagg
     6061 aggagccatt gcatatggtt tagatatcgt agacggtaaa tttgatgg
//
LOCUS       DAVBHS010000119         6049 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVBHS010000119 DAVBHS010000000
VERSION     DAVBHS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.119"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..282
                     /locus_tag="VQ651_001652"
     CDS             <1..282
                     /locus_tag="VQ651_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HET2754617.1"
                     /translation="YYTAIHKNFDLPYDIYRPESQNPRTQIEILQKNGELAELSSLSP
                     IVQSLAGSRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            295..1101
                     /gene="yidA"
                     /locus_tag="VQ651_001653"
     CDS             295..1101
                     /gene="yidA"
                     /locus_tag="VQ651_001653"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HET2754618.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRPMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            1144..2142
                     /locus_tag="VQ651_001654"
     CDS             1144..2142
                     /locus_tag="VQ651_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HET2754619.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNELECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLTVVELKTILSKHN
                     LATSGKKAELITRIINNVNIDNLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDISMED
                     YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHA
                     LIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECYSPLNNLELDDFE"
     gene            complement(2309..2380)
                     /locus_tag="VQ651_001655"
     tRNA            complement(2309..2380)
                     /locus_tag="VQ651_001655"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2345..2347),aa:Arg,seq:cct)
     gene            complement(2423..2770)
                     /gene="rplS"
                     /locus_tag="VQ651_001656"
     CDS             complement(2423..2770)
                     /gene="rplS"
                     /locus_tag="VQ651_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET2754620.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(2889..3218)
                     /gene="crcB"
                     /locus_tag="VQ651_001657"
     CDS             complement(2889..3218)
                     /gene="crcB"
                     /locus_tag="VQ651_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET2754621.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(3212..3586)
                     /gene="crcB"
                     /locus_tag="VQ651_001658"
     CDS             complement(3212..3586)
                     /gene="crcB"
                     /locus_tag="VQ651_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET2754622.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(3583..3849)
                     /locus_tag="VQ651_001659"
     CDS             complement(3583..3849)
                     /locus_tag="VQ651_001659"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET2754623.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSYILKRSRDYQDQNIK"
     gene            complement(3965..4408)
                     /locus_tag="VQ651_001660"
     CDS             complement(3965..4408)
                     /locus_tag="VQ651_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HET2754624.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            4512..5447
                     /locus_tag="VQ651_001661"
     CDS             4512..5447
                     /locus_tag="VQ651_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET2754625.1"
                     /translation="MEICQQILEKIKEYNTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNVTDAETAAIVGAPGRIDSVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            5501..5785
                     /locus_tag="VQ651_001662"
     CDS             5501..5785
                     /locus_tag="VQ651_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HET2754626.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(5842..>6049)
                     /locus_tag="VQ651_001663"
     CDS             complement(5842..>6049)
                     /locus_tag="VQ651_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HET2754627.1"
                     /translation="PRFEALEQAIASKDLEKVREAFKKMNSTWTINESVVRDNSTAHY
                     GRVETAISFLPSSMETEPTDESGT"
BASE COUNT         1998 a         1279 c         1045 g         1727 t
ORIGIN      
        1 tactatactg ccattcataa gaactttgac ctcccttatg atatctatcg tcccgaatct
       61 caaaatccac ggacacagat tgagatttta caaaaaaatg gagaactggc cgaactctct
      121 agcctgtctc ctatcgtcca atcccttgct ggcagtcgcc acggagataa tcgtttctat
      181 ttccctaaag aaatgttaga ccaaaacagc atcttcgcaa gcattaccca gcaattttta
      241 cacttgattg agaacgatca ttttacccca aataaaaact agaagaggaa ttttatgagt
      301 attaaactaa ttgccgtcga tattgacgga accctagtca acagccaaaa ggaaatcact
      361 cctgaagtct tttctgccat ccaagatgcc aaagaagctg gtgtcaaagt cgtgattgca
      421 actggccgcc ccatcgcagg tgttgccaaa cttctggacg acttgcagtt gagagaccaa
      481 ggtgactatg tggtgacctt caatggtgcc cttgtccaag aaactgctac tggccatgag
      541 attatcagcg aatccttgac ctatgaggat tatctggata tggaattcct cagtcgcaag
      601 ctcggtgtcc acatgcatgc cattaccaag gacggtatct atactgcaaa tcgcaatatc
      661 ggaaaataca ctgtacacga atcaaccctc gtcagcatgc ctatcttcta ccgtacccct
      721 gaagaaatgg ctggcaaaga aattgttaaa tgtatgttta tcgatgaacc agaaattctc
      781 gatgctgcga ttgaaaaaat tccagcagaa ttttacgagc gctactccat caacaaatct
      841 gctcctttct acctcgaact ccttaaaaag aatgtagaca agggttcagc cattactcac
      901 ttggctgaaa aactcggatt gaccaaagat gaaaccatgg caatcggtga tgaagaaaat
      961 gaccgtccca tgctggaagt cgttggaaac cccgttgtca tggaaaatgg aaatccagaa
     1021 atcaaaaaaa tcgccaaata catcaccaaa acaaatgacg aatccggcgt tgcccatgcc
     1081 atccgaacat gggtactgta aaagtatcat ttttcaataa gaattgatta gcaataaaat
     1141 ccaatgaatt tttttagcaa actatttaat ttaaaacaaa ataatcataa tagagacaca
     1201 aattctgatt gtaacaattt ttacctaaac gaattagaat gtggccttac tcctgggcaa
     1261 ctcatactca tagattggac tcaaaaaaca gggagaaatt ataatttccc aagatatttt
     1321 aaatactctc ttcaaattga ccctgaatct acacacaatc aattatacaa attaggatac
     1381 ttcactaaaa ataagacttt atcatatctt acagtagtag aattaaaaac tatattatct
     1441 aaacataatt tagctacttc tggaaaaaaa gcagaattaa ttacaagaat aattaataat
     1501 gttaacattg acaatttaga tattccgttc gaatttaaac taacaaaaga agcacaaaat
     1561 cttattatcg aacatagtga ctatatcaaa gcatactatg ataaagacat aagtatggaa
     1621 gattattgta aagaaaaaaa caatatctct tttaaagcaa cttttggtga tataaaatgg
     1681 agtctcttaa ataaacaagc tcataggaat actgtatcag gagattttgg atgcttatct
     1741 aacacacgaa aggctcaggg aagacatttg gaacaagaag gtaatattaa acatgcttta
     1801 atatattaca tagaatcttt gataattact atttcaggat tagaaaacaa tttttcagcc
     1861 actgattatc cagtatatta tcccgattcg atacctgact actcactaaa acatattcaa
     1921 acattaatgg aatcattatc tgatgacgat tatgattttg cttttgatga agcattattt
     1981 cgcttctcaa ttttgaatgc aaatcatttt ttatctaagg aagatattga ctatttaaga
     2041 gttaatttac ctcgttccac tgctgaagaa ataaacaatt acttaaagaa atatgaatgt
     2101 tatagtcctt taaataattt agaacttgac gattttgaat aaattgacta tacaaacatt
     2161 tatatactcg atatagtctc aattttatct gatgattgcc caaatttttc aataataaaa
     2221 cgcataatat tatggagaca atcccctata ttatgcgttc ttttaatatc aaagactctt
     2281 tgacaaactt ctttgatatc taattacatg ccccctgcag gaatcgaacc tgcaactact
     2341 ccttaggagg gagttgttat atccattgaa ctaagggagc tagataaaaa ctctgctgaa
     2401 aaagcagagt tttttagtcg aattaacgac ggatttcttt gatacgagct gccttacctt
     2461 gaagagcacg caagtagtac aatttcgcac gacgtacttt accgtaacgt acaacttcga
     2521 ttttttcaac acgtggagtg tggattggga agatacgctc aacacctaca ccgttagaga
     2581 ttttacgaac tgtgtagttt tcagagatgc cagcaccttt acgtgcaata acaacacctt
     2641 caaaaatctg gatacgttca cggttacctt cgacaacttt cgcatgtaca cgaacagtgt
     2701 caccaggacg gaatgatggg atatctgtac gaagttgacc ttcagtcaag ctttggatta
     2761 atggattcat tttattctcc tatctttgtc aatcttgagg aatcttcctc agcggataaa
     2821 ctgtattttt gtgcgtccat tacacacaag atatagttta ccaaatttct aaaaaaaagt
     2881 aaagaaattt atagcaaaat tcctaaaaaa atcgcaacca aaccacctat gtaagtcaaa
     2941 gtcaggtaag aataaaagac cttcttatca cttagcagtc tttgaagttc gtcattcaag
     3001 gtcgaaaaag ttgttaaacc gccacaaaat cctgttgcta gaatagcata tacttcctta
     3061 gactctacat gattgtagaa tactccaatt aaaaaacaac ctaaaagatt agctatgagc
     3121 gttccgagtg gcaatttaga agcttgatta tagcgagaaa agaaataccg tacgagtgct
     3181 cccaagccac aggcgattgc aagatagacg attaccattt cttcctcccc aaatagtaag
     3241 ctaaaagcag gcctccaccg atactcaaaa gcaaatacag gactaaacta aaataacgcc
     3301 ctgtatcaag cagcttcaca gtatcaagca ttaggctgga aaaagttgtt aaacctccgc
     3361 aaaaacccgt ccccagtgct aaaatcaagc ccttactagt ccccttatag accagatagc
     3421 ccttgacaag aaaaatcaag cagaaaattc ccagatagtt gacaaagaga gtgccccaag
     3481 gaaagtctgg actggctggt aaccaggtgg aaactagata tcggacaagt ccgcccaaca
     3541 tagcagctag aaaaattcct agcggataaa attgttcttt tttcatttga tattttgatc
     3601 ctgataatca cgcgaacgtt tgagtatgta cgaaaaagtc gcgacaatag tctcctgata
     3661 gcgcttgtcc tctacacgac tcctaacctt ttcaaaaata acttcttctc tcttagtatc
     3721 taaaatcggt ttacctgaag ctttcttata agcaaccacc ccctcaacta aatgcattcg
     3781 ttcttctaac agtttgacaa tttggtcgtc gatttgatcg atttcttgac gaataatatc
     3841 taaatccata cagtctcctc ctttatttga attattgtat caaaaagcca tcaattaggc
     3901 tagtaaaatc ccaactctcg ataagaaaaa tgcactgatt gattacatca gcgcactttt
     3961 atgcttatcc tactttagca gccaattctt ctgcgaattg ttccaagcgt tcaatatctt
     4021 cttcctcggc agaaagatca actttaacac actctgaacc tttttctgct cctgttgaca
     4081 caaagacacg gtcaaagtca tcaacagcct tacagaattc atcgtagaag gtatctcctg
     4141 agccgaccac tccgtagatt ttgccattca agttgagatc tgctaggtct tcgtagaagt
     4201 ccatcatctc atctggcaat tcaccatcac cataagtata ggtcgcaacg atagcgatgt
     4261 ctgcttccaa gaagtctgaa gcgtcaacag ttgtacattc atcaacatca acatccaagc
     4321 ccaagtcacg taatttgtct gctacaatat ctgcaatttc ttcggtatta ccggtcatac
     4381 tggcaaatac aatttttgct aatgccatat cgtcctcctc aatttatctt tctccattat
     4441 atcacatctt tttcatttta aaaataaaaa ttcttgtgct acaatgaaat gagaaagaat
     4501 tgaggttatt tatggagatt tgccaacaaa ttttagaaaa aattaaagaa tacaacacca
     4561 ttatcattca tcgtcatatg aaaccagacc ctgatgcctt gggaagtcag gtgggattga
     4621 aagccttgct ggaacatcat ttcccagaaa aaaccatcaa agccgtcggt tttgatgaac
     4681 caactcttac ttggatggct gagatggatc ttgttgaaga tagagcctac caaggcgcac
     4741 ttgtcatcgt ctgtgataca gctaatactg ctcgtatcga tgataagcgc tatagtcaag
     4801 gtgattttct cattaagatt gaccaccatc caaatgatga tgtatacggt gacctgtctt
     4861 gggtcgatac tagttcaagt agcgctagcg agatgattac cctatttgcc caaacaaccc
     4921 aactagcctt ggcagatcgc gatgctgagt tgctctttgc aggaattgtc ggtgatacag
     4981 gtcgcttcct ctacccttct accactgcac ggactcttcg cctggctgct tatttgagag
     5041 aacataactt tgactttgcg gctctcactc gcaaaatgga cactatgagc tacaaaattg
     5101 ctaaactgca aggctacatc tacgaccatc tggaagtgga tgaaaatggt gctgctcgcg
     5161 ttatcctgag tcagaaaatc ttgaaacaat acaatgtaac cgatgctgaa actgcggcca
     5221 ttgtaggtgc acctgggcgt attgatagtg ttagtctctg gggaattttt gtggaacagg
     5281 ctgatggcca ctaccgcgtt cgcttacgca gtaaaatcca tcctatcaat gaaatcgcca
     5341 aggagcatga tggtggaggc caccctctag caagtggtgc taattcctat agcctagaag
     5401 aaaatgaaat catctaccaa aagttaaaaa acttgcttaa aaactgataa aatacttgcc
     5461 aaacttttca gaatctgata gactagtatg gtaacaatct atggctcgca aagagaccat
     5521 ggcagaaagg aaatattgca aaatgaaaaa agatatccat ccagaatatc gcccagttgt
     5581 cttcatggac acaactactg gttaccaatt ccttagcggt tcaacaaaac gctctaacga
     5641 aacagttgag ttcgaaggcg aaacttaccc attgatccgt gtggaaattt catcagactc
     5701 acacccattc tacactggac gtcaaaagtt cactcaagca gatggacgcg tggatcgttt
     5761 caacaaaaaa tacggtctca aataatgata aaagaacagt ttcgactgtt cttttttgtt
     5821 tcttgaaatc aactgctgtt ttcatgttcc agactcatct gtaggttcgg tttccatgct
     5881 actaggcagg aaggaaatag ctgtttcaac acgtccataa tgagctgtac tattgtcacg
     5941 aaccacactt tcattgatgg tccaagtgga attcattttc ttaaaagctt ctcggacttt
     6001 ttccaaatct ttggaggcaa tggcctgttc caaggcttca aaacgagga
//
LOCUS       DAVBHS010000120         6008 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVBHS010000120 DAVBHS010000000
VERSION     DAVBHS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.120"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(48..797)
                     /locus_tag="VQ651_001664"
     CDS             complement(48..797)
                     /locus_tag="VQ651_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET2754628.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            930..3266
                     /locus_tag="VQ651_001665"
     CDS             930..3266
                     /locus_tag="VQ651_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET2754629.1"
                     /translation="MDKNKIMGLTQREIKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAVASILTSYMA
                     HSEDKNPTAQAIRQRFQGQVVYPMLSNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLNY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELIALAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            3368..4045
                     /locus_tag="VQ651_001666"
     CDS             3368..4045
                     /locus_tag="VQ651_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HET2754630.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            4032..4829
                     /locus_tag="VQ651_001667"
     CDS             4032..4829
                     /locus_tag="VQ651_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET2754631.1"
                     /translation="MLASEVIHAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            4846..5949
                     /locus_tag="VQ651_001668"
     CDS             4846..5949
                     /locus_tag="VQ651_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET2754632.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
BASE COUNT         1630 a         1207 c         1470 g         1701 t
ORIGIN      
        1 tgatatataa atgaaaacct tagctgtttg gccaaggttc atttcttcta tgcaagttct
       61 tctcttttct tatctgggtt ccagatgaag cttgcgataa agctaaagat gatggttagg
      121 atagcgagaa taatagcaag gatgagggca gggatgcgga taaaccacca gagtggattt
      181 ggttttttat cattgtgcca ttgtttcgtt tcttcgtcca gacgttggaa ttctgtttga
      241 atacgattgg cgactgtttc aatgccttca tcattcattt tcttgatatc tgagatatcg
      301 attggatttc caaagttcat atcgacacgt tcacggctaa tcaagccctt caaagtcatg
      361 ggaccggtgt aggtaaccgg catgatacgg accttggcca ttttggcaat cagtgctgcg
      421 ccccccttga catcgtttga gtggcggcta ccacttggaa acatgatgag agagcggtca
      481 ctttttttga gaacgttgat aggatatttg atggctgagg cgctgggatt ttcacggtcg
      541 atgggaaagg cgccacacat acgaatccac caaccaaaga tacggttggt aaagagttct
      601 ttttttgcca taaagatgaa ctgttttggc ttggtcgcaa aggccatata aacaggatcc
      661 caccaggtac ggtgaggcgc aactaaaata taattttcat cttgattagg aattttatca
      721 gtattatgat agtgagcatt gccattgatg gaccataaga gcaatacaac taatccacgc
      781 aaataagtat aaaacatgcg atctccttcg attgttttct tgttattatt ataccttatc
      841 aaaggagggc tggcaaactt ttcccttgac tagatgcata tttgggatga aattagaatt
      901 ctgttaaaaa aaatgatata atagaattta tggataaaaa taagattatg ggattaaccc
      961 aaagagaaat caaggaaaga caggctgagg gtttggtcaa tgactttacc gcatcagcca
     1021 gtaccagcac ttggcaaatc gttaaacgaa atgtctttac cctttttaac gctttgaact
     1081 ttgccattgc tttggccctt gcctttgtgc aggcttggag caatctggtc ttctttgctg
     1141 ttatctgctt taacgctttt tctgggattg tgaccgagct acgagccaaa cacatggtgg
     1201 acaagctcaa tctcatgacc aaggaaaagg tcaaaaccat ccgtgatggt caggaagttg
     1261 ctcttaatcc tgaagaatta gtgctaggag atgtcattcg tttgtctgca ggagagcaga
     1321 ttcctagtga tgccttggtt ttggaaggct ttgcggaagt caatgaagcc atgttaacgg
     1381 gagaaagtga tttggtgcaa aaggaagttg acggcttact tttgtcagga agtttcctag
     1441 ccagtgggtc agttttatct caagttcacc atgtcggtgc agacaactat gctgccaaac
     1501 tcatgcttga ggctaagacc gttaaaccca tcaactcccg tatcatgaaa tcgctggaca
     1561 agttggctgg ttttactggg aagattatca ttccctttgg tctggctctc ttgctggaag
     1621 ccttgctttt aaaaggcctg cctctcaagt catccgttgt aaactcgtcg acagctcttt
     1681 tgggaatgtt gcctaaggga attgcccttt tgaccattac ttcgctcttg actgcagtga
     1741 ttaagttggg cttgaaaaag gtcttggtgc aggagatgta ctctgttgag accttggcgc
     1801 gcgtggatat gctctgtctg gacaagacgg gtaccatcac ccaaggaaag atgcaggtgg
     1861 aggctgttct tccactgacg gaaacttatg gtgaagaggc tgttgccagc atcctgacca
     1921 gctatatggc ccatagtgag gataaaaatc caacagctca agccattcgc cagagattcc
     1981 aaggtcaagt agtctatcct atgctctcta atcttccctt ctccagcgac cgcaagtggg
     2041 gagccatgga gttagaaggt ttgggaatag ttttcttagg ggcacctgag atgttgctgg
     2101 attctgaggt cccagaagcc agagaggcct tggagagagg atcacgtgtc ttggtcttag
     2161 ctctcagtca ggagaaatta gaccatcaca aaccacagaa accatctgat attcaggctc
     2221 tagccttgct ggaaatcttg gatcctattc gagagggagc ggcagagacg ctgaactatc
     2281 tccgttctca ggaagtgggg ctcaagatta tctctggtga caatccagtt acggtatcca
     2341 gcattgccca gaaggctggt tttgcggact atcacagcta tgtcgattgt tcaaaaatca
     2401 cggatgagga attgattgct ctggcggagg agacagcgat tttcggacgt gtttcccctc
     2461 atcaaaagaa actcattatc caaacgctga aaaaagcggg tcatacaaca gctatgactg
     2521 gggatggagt taatgatatc ctggctcttc gtgaggcgga ttgttctatc gtaatggcgg
     2581 agggagaccc ggcaacccgt cagattgcca acctggttct cttgaactca gactttaatg
     2641 atgttcctga gattcttttt gaaggtcgtc gtgtggtcaa taacattgcc cacatcgctc
     2701 cgattttctt gataaagacc atctattcct tcctgttagc agtcatctgt attgccagtg
     2761 ctttactagg tcggtcagag tggattttga ttttcccctt cattccgatc cagattacca
     2821 tgattgacca gtttgtggaa ggtttcccac cattcgttct gacttttgag cgaaatatca
     2881 aacctgttga gccaaatttc ctcagaaaat ccatgcttcg tgccctacca agtgccctca
     2941 tggtcgtgtt tagcgttctt tttgtgaaaa tctttggaac gagtcaaggt tggtctgagt
     3001 tagaaatctc aactctcctc tattatctct tggggtcaat tggtttctta tccgtattta
     3061 gagcctgcat gccattcact ctatggcgag tcctcttgat tgtctggtca gtaggaggct
     3121 tcctagccac agctctcttc ccaagaatcc aaaaactgct tgaaatttca accttgacag
     3181 aacaaacgtt gcctgtttat ggtgtcatga tgttggtctt tacagtgatt ttcatcctga
     3241 ctagtcgcta tcaagctaga aaataaagaa agactgcaat ctgtggattg cagttttttt
     3301 aggtacaaga ttgccagccg aaatgtggta taataagagg taatagagtt ttggaaagtg
     3361 agagaagatg atttcaaaga gattagaatt ggtagcttcc tttgtgtcac agggggctat
     3421 tttactagat gtgggaagtg accatgctta tctgcctatc gagttggttg agagaggcca
     3481 aatcaaaagc gctattgcag gtgaggtggt ggaaggtccc tatcagtctg cggttaaaaa
     3541 tgttgaggct cacggcctaa aggagaaaat ccaagtccgt ttagccaatg gcttggcagc
     3601 ttttgaagag actgaccaag tgtctgtcat taccattgct ggcatgggtg gtcgtttgat
     3661 tgctaggatt ttagaagaag gtttggggaa gttagctaat gtagagcgtt tgatcctcca
     3721 gcccaataat cgtgaagacg acttgcgtat ctggctacag gatcatggat tccagattgt
     3781 agcagaaagc atcttagaag aagctggaaa gttttatgag attttggtgg tggaagcagg
     3841 acaaatgaag ctatcagcca gtgatgttcg ctttggtccc ttcttgtcca aagaagtcag
     3901 tccagtattt gtccaaaaat ggcaaaaaga agctgagaag ctagagttcg ctctcggaca
     3961 aatcccagaa aaaaatctgg aagaacgtca agttctagta gataagattc aagctatcaa
     4021 ggaggtgctc catgttagca agtgaagtga ttcatgctta tgaagccttt tgccctcagg
     4081 aattttctat ggagggagac agccgtggtc tgcaaattgg cactttagac aagggtatcc
     4141 aaagggtcat ggttgccctc gatattcgtg aagagacagt ggcagaggcc attgaaaagg
     4201 gtgtggactt gattatcgtc aagcacgcgc ctatctttcg tcctatcaag gacttgcttg
     4261 ccagccgtcc gcaaaatcag atttacatcg acctgattaa gcatgacatc gcagtttatg
     4321 tcagccatac caatattgat atcgttgaaa atgggctcaa tgactggttt tgtcagatgc
     4381 taggaatcga ggagacgact tatctgcagg aaacaggtcc agaacgtgga attggacgta
     4441 ttgggaatat tcagcctcag acattttggg aattggccca acaggtcaag caagtctttg
     4501 acctagatag ccttcgaatg gtgcattatc aagagaatga tttgcagaag cctatttcaa
     4561 gagtagcaat ttgtggtgga agcgggcagt ctttctataa ggatgctttg gcaaaggggg
     4621 cagatgtcta tatcactggt gatatctact accatactgc tcaggatatg ctgtctgatg
     4681 gcttgttagc attggatcca ggtcactata tcgaagtgct ttttgtggaa aaaatcgcag
     4741 cactccttac tcaatggaaa gagaaaaaag gctgggaact agagatttta cctagtcaag
     4801 catcgaccaa tcctttccgt catatctagt tagaaagtga agacaatgaa aaaagttgcc
     4861 attattggag caggaattgt gggagcaaca gctgcctact acctctcgag agaaagtgac
     4921 ctagaggtga ccgtttttga ccatgagcaa ggtcaagcca ccaaggccgc agcaggaatt
     4981 atcagtcctt ggttttccaa acgccgtaat aaagcctggt acaagatggc gcgcttgggg
     5041 gctgattttt atgtggattt attagctgat ttagagaaat caggacaaaa aatcgacttt
     5101 taccagcgtt cgggagtctt tctcttgaaa aaggatgaat ccaagttgga agaactttat
     5161 caactggccc tccagcgcag agaagaatct cccttgatag ggcaattagc cattctggac
     5221 caagcctcag ctaatgaatt attccctggt ttgcagggat ttgaccgcct gctctatgct
     5281 tctggtggag cgagagtaga tggccaactc ttggtaactc gtctgctaga agccagtcat
     5341 gtcaagctgg tcaaagaaaa agtgactctg acaccgttag catcaggcta ccagattggt
     5401 gaagaggagt ttgagcaggt tattttggcg acgggagctt ggttggggga catgttagaa
     5461 cccttgggtt atgaagtaga tgttcgtcct caaaaaggac aactacgaga ttatcagctg
     5521 gtccaagaca tggaagctta ccctgttgtc atgccagaag gggagtggga tttgattcct
     5581 tttgcaggtg ggaaattgtc cttaggcgct acccacgaaa atgacatggg atttgatttg
     5641 acggtagatg aaaccttgct ccaacaaatg gaggaggcca ccttgactca ctatctgatt
     5701 ttggctgaag ctacttcaaa atctgagcgt gttggaatcc gtgcctacac cagtgatttc
     5761 tctcctttct ttgggcaggt gcctgactta actggtgtct atgcagccag tggactaggt
     5821 tcatcaggcc tcacaactgg tcctattatt ggttaccatt tagctcaact gatccaagac
     5881 aaggagttga ccttggaccc tgaaaactac ccaattgaaa actatgtcaa acgagtaaaa
     5941 agcgaataat actcaatgaa aatcaaagaa caaactagga aactagccgc gggttactca
     6001 aagcactg
//
LOCUS       DAVBHS010000121         5939 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVBHS010000121 DAVBHS010000000
VERSION     DAVBHS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.121"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            405..1310
                     /locus_tag="VQ651_001669"
                     /pseudo
     CDS             405..1310
                     /locus_tag="VQ651_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            1355..1795
                     /locus_tag="VQ651_001670"
     CDS             1355..1795
                     /locus_tag="VQ651_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2754633.1"
                     /translation="MNFVLSSLSEGLLWSIMAIGVYLTFRILDIADMTAEGAFPMGAA
                     IVVSQIQAGANPWLATLLALLAGMVAGLISGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGAVFSLSLVCLLLVWF"
     gene            1804..2232
                     /locus_tag="VQ651_001671"
     CDS             1804..2232
                     /locus_tag="VQ651_001671"
                     /inference="COORDINATES: protein motif:HMM:NF014684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754634.1"
                     /translation="MKTEIGLVLRSTGDNIPMSEANGVNVNTMKIVGYMISTGLIALC
                     GSLFAQNDGFSDVTSGTGTIVVGLSSEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYE
                     IPNLDQNLVRLFNAILLALVLFAPALQKRLKIRGLKLRNE"
     gene            2245..3048
                     /locus_tag="VQ651_001672"
     CDS             2245..3048
                     /locus_tag="VQ651_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2754635.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKHLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKLKLLLLDEHTAALDPKTSQMVMDLTQKIVEYHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(3081..3791)
                     /gene="treR"
                     /locus_tag="VQ651_001673"
     CDS             complement(3081..3791)
                     /gene="treR"
                     /locus_tag="VQ651_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HET2754636.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            3976..>5939
                     /gene="treP"
                     /locus_tag="VQ651_001674"
     CDS             3976..>5939
                     /gene="treP"
                     /locus_tag="VQ651_001674"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HET2754637.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGNDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATR"
BASE COUNT         1657 a         1104 c         1382 g         1796 t
ORIGIN      
        1 ctgaaagaat tttcgaaaaa ttttaaaaaa ttttgaaata ttattgacag gtgatgaaaa
       61 aggtggtagg ataggaagga tcaaaaaaga aagcagaaaa agaaatgaat aagagacaag
      121 ccgtaactat gaatcaaaac ttttacttta gctactttag atagtgtttg tagacatgtc
      181 accatggatg caaacagttc aagcaatttg tttgtatcta tgtggctaag atttttgatt
      241 gtggtccata tagatgaata tctaaatgga ccagctaagt tgtaacttgt taggttattt
      301 caagtacccg tttagatagt atctaggcgt ttttttgttt tatcttttct gagaaggagt
      361 ttcattatga aagttgttcg aaaatcacta gccccctctt ggtagtgggg atcctcttga
      421 cttctctgat cagtttgcat cagttgaagg cagataagaa aagagatgtc tttcgtatcg
      481 gtatttcgca gtttattacc caccagtcct tagacgctac tagagaagga tttgtggatg
      541 agctggccaa acaaggctat gttgaggggg aaaatattga gattgatttg caaaatgcac
      601 agggagaaca aagaaatcta aaaacgattt cccagcaact agcagaatcc agcgatgtcg
      661 ttctagccat cgcaaggcct tctgctcaga gcttggccaa tacgacgcaa acgacaccag
      721 ttatcttttc agctgtaaca gacccagtca gtgccaagtt ggttgagtca agagaacatc
      781 ctgggggcaa tgtaactggg acaagcgatc agtcatcaga tgccatttca acccaaatca
      841 atttgataaa gaaagtgttg ctaaaggcta aaacgattgg aatcctctat actcagagcg
      901 agcccaattc ggttgtttaa aaggatgaag ctaagcgatt tctgaaagaa aaaggcttta
      961 ccgttgttga aaaaacaatc ttggacagta ataacgtcaa ggcggcagca gagagtttga
     1021 tggcagaggt ggatatggtt tttgtcccaa ctgataatat catttcgtca accatggaaa
     1081 cagttaagca ggtttctatt aaacacaagg ttccagtatt cggtggttca acagaaatga
     1141 ttgctgttgg tggcttgtat aactacggaa ccaattatga agaattggga agacagacag
     1201 ctcgcatgct gattcgtgtt ttaaaaggtg aggaaccaga aaatatagca gttgagttgc
     1261 ctgaaaaact ggaattgcat actaatcaag aaatggcaga tgcattgtga attgatatta
     1321 gtaagttaga aggcaaggaa taaggaggat taagatgaat tttgtgttat ctagtttatc
     1381 agaaggtttg ttgtggtcga ttatggcaat tggggtctac ttgactttcc gtattttgga
     1441 tattgcggat atgactgctg agggagcctt tcctatgggg gcagcaatcg ttgtatcaca
     1501 gatacaggca ggggcaaatc cttggcttgc gactttactt gctttgctgg caggtatggt
     1561 agcaggtctt atatcaggaa tgctccacac caagatgaaa atcccagctc tcttgacagg
     1621 gattgtgacc ttgacagggc tctattcaat caatattaaa atcatgggca gtgtacccaa
     1681 tctttccttg ggagattctg caaccgtctt taaacagttg gcgagcttgg gtctgacaaa
     1741 tgaaggagct gttttctcac ttagtttagt ctgtctctta cttgtttggt tttgaccctt
     1801 ttgatgaaaa cagagattgg tttggtcttg cgttcgactg gggacaatat cccgatgagt
     1861 gaggccaatg gagtcaatgt aaacaccatg aagattgttg gttacatgat ttcaaccggt
     1921 ttgattgccc tatgtggttc cttgtttgcc caaaatgatg gattttcaga tgtgacttct
     1981 gggacaggaa ccattgttgt tggtttgagc tcggaagtct tgatacacga cttgaccatt
     2041 ggaggccgct tattatccat cggaatcggt gctattgttt accgtttgat tattttaaat
     2101 atctatgaaa ttccaaatct agatcaaaac ttggtacgtc tctttaatgc aatcttgctc
     2161 gctttggttt tatttgcacc agcattgcaa aagagattaa agattcgtgg tctgaaattg
     2221 agaaatgaat aggaggagaa agttatggca agtttgttaa cacttgaaaa tattcacaag
     2281 acatttgaag cagggacggt caatgagaac catgtcctca aaggattaga tttagaggtt
     2341 gaagagggag attttatctc tgtgattggt ggaaatggag caggaaaatc cactttgatg
     2401 aatatcttgg ctggtaatct atcggtggac gaaggggacc ttctactggc agggaaatcc
     2461 attaaacatc tgagtgtcag aaagagagcc aaggatattg cccgtgtttt tcaagatcct
     2521 aagatgggaa cagcttctcg tttgacgatt gaggagaata tggctattgc cttgagacgt
     2581 gggcaaaaga gagggcttgg ttggggcgtg aaggagaagg atagaatcca gttccaagag
     2641 gccttgaagg agttgaatat tggtcttgaa aaccgcttaa aagtagatac tcaatatctc
     2701 tcaggaggac aaagacaggc tttgacccta gtcatggcag ctctggtgaa actcaaactt
     2761 ttgcttttgg atgaacatac tgcggcactt gacccgaaaa ccagtcaaat ggtgatggac
     2821 ttgacgcaaa agattgtgga gtaccatcag tggacgactt tgatgattac ccatgatatg
     2881 aatcatgcga ttgagtacgg caatcgtctc attatgctct atcaaggcaa gatagtggtg
     2941 gatgtcaagg gagaagagaa aaagcatctg acagttgaag accttatgca tctcttccag
     3001 aaaaatagtg gccaaagtct ggtcagtgat gaattagttt tgggataaag aaaaaggctg
     3061 agatctggtg tctcagtctt ttatttcttg cgttttgcaa aatctacaaa tctaaatttt
     3121 tctaacttat ggcgactttc ggtaaactga aattggcgcc cgtcttggag atagactttt
     3181 gatttaatcg aaacgacata agggtctttg ccaatgtcca tgagaatctt gtctcggtcg
     3241 cttgagtggt caatggtgat ttccttctga gcataatcaa taaggagttt gaggccattt
     3301 tctatataag aatagataga ctgctcagca atttggcgag tgagatttgg gatgagttcc
     3361 atatccagat agtccgtatc cagaacggat accagatcat ccaccacacg ctggcggacc
     3421 accttccaaa ccatccgaaa ctctgggaaa ccggttatca gtgaggattt tttatcaata
     3481 ataatcttgt ccagactgac cacgttggtt ttagagcgca gtccaagttc tttaactagt
     3541 tcttggtagc tggttaggtt ggatacaggg aaattgacgg tttcttcttt gacgacttga
     3601 gaaccttgcc ctcttatctt tttgatcaat ccttcctctt ggaggagaga cagggccttt
     3661 cggacggtat cacgactcac ttgatattgc tccataaggt cgtgctcgct aggaaggaaa
     3721 tctccgacag cgtaagtctc gttttgaatg gtttcttgga tttgcttaaa taattgttgg
     3781 tatttcttca ttttgcctgc ttaaataggt ttttagtcta gtttaatgaa cacctgtatc
     3841 tattttacca tacaagtgct agaaaattta caaaaaaaca ccctttttat accaaacaag
     3901 ttgcagacaa gtttggtatc gtttacaata tacttatcaa atcaaacttg ctttgacaag
     3961 tataaggaga atcaaatggg aaaatttgaa caagaagcca aagatctgct tcaggcaatc
     4021 ggaggcaaag aaaatgtgac tgccgtaact cactgtgcaa cacggatgcg ctttgtttta
     4081 ggaaatgata agaaggctaa tgttaaagct atcgagtcaa ttccagctgt taaaggaacc
     4141 tttacaaatg caggtcaatt tcaggtaatc attggaaatg acgtgcccat cttttataat
     4201 gattttacag ctgtttcagg tattgagggt gtttccaaag aagcagccaa gtctgcagct
     4261 aagagtaatc aaaacgtggt ccaacgtgtc atgaccactc tggcggagat ttttactccg
     4321 attattccag ccttgatagt cggaggattg atcctcggtt tccgtaatgt cttggaaggt
     4381 gtccattggt cgatgttgga tggcaagacc atcacagaat cctctcagtt ttgggcaggt
     4441 gttaatcatt tcctctggtt gcctggtgaa gctatcttcc agttcttacc agtagggatt
     4501 acttggtctg tttctcgtaa gatgggaacc agccaaattt tgggaattgt tctcggaatc
     4561 tgtttggtat cgcctcagtt gctcaatgcc tatgcggttg cttcaacgcc agcagctgat
     4621 atcgcggcaa actgggtttg gaattttggc tattttactg ttaatcgtat cggttaccaa
     4681 gcccaagtta tcccagcctt gcttgcaggt ttgagtctat cttatcttga aatcttctgg
     4741 cgcaagcata tcccagaagt catttctatg atttttgtac ctttcttgtc attgattcca
     4801 gccttgattt tggctcatac tgttttggga ccaatcggtt ggacaattgg acaaggactt
     4861 tcatcagttg tcttggcagg tttaactggt ccagttaaat ggctcttcgg tgcaattttt
     4921 ggtgccctct acgctccatt tgtcatcaca ggtctgcacc atatgaccaa tgccattgat
     4981 acacaattga ttgcggatgc tggtggcact gccctatggc caatgattgc tctttctaat
     5041 attgctcaag gctcagccgt gtttgcctat tatttcatgc atcgccatga tgagcgtgag
     5101 gctcaggttt cacttcctgc aaccatttca gcctatctcg gtgttacaga accagctctt
     5161 tttggggtta acgtaaaata tatttatcca tttgttgctg ggatgactgg ttcagccctt
     5221 gcaggcatgt tatccgttac ttttaatgta actgcggctt ctattggtat cggtggtttg
     5281 ccaggtattc tctctattca acctcaatac atgctgccat ttgcaggaac tatgctagtt
     5341 gcgattgttg ttccaatgct cttgactttc ttcttccgca aggctggtct ctttacaaaa
     5401 acagagggcg atacgaactt gcaggcagaa ttcgttgctc aagaagaagc agaatttgtg
     5461 aaccatgaac cagtagaact tacttcggta gaaattatca gcccactaac tggccaagtg
     5521 aaagaattga gtcaagcgac ggatcctatt tttgcatcag gtgtcatggg gcaaggtcta
     5581 gtcattgaac caagccaagg tgagttgacc tctccagtta atgggacagt gacggttctt
     5641 ttccctacca agcatgccat cggcattgtc tctgacgagg gagttgaatt gctcatccac
     5701 atcggtatgg atacagtagg tcttgatggc aaaggttttg aaagtcttgt agtccaagga
     5761 gatcacgtta cagttggtca gcaactgatt cgttttgata tggatgtcat taaggctgca
     5821 ggtctggtga cagaaactcc tgttatcatc accaaccaag atgcttatac agcgactatt
     5881 cccggaactt atccgacaac gattcaagct ggagcatctc tcatggtcgc tacacgaat
//
LOCUS       DAVBHS010000122         5799 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVBHS010000122 DAVBHS010000000
VERSION     DAVBHS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.122"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..1647
                     /locus_tag="VQ651_001675"
     CDS             <1..1647
                     /locus_tag="VQ651_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET2754638.1"
                     /translation="NYRTDKTAMWALSGNFDASKSWKKEELELANWISKNYSINPVDN
                     DFRTTTLTLKGHTGHKPQINISGDKNHYTYTENWDENTHVYTITVNHNGMVEMSINTE
                     GTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVDYNGDPNRAVDGNRNGNFNSGSVTHTR
                     ADNPSWWEVDLKKMDKVGLVKIYNRTDAETQRLSNFDVILYDNNRNEVAKKHVNNLSG
                     ESVSLDFKEKGARYIKVKLLTSGVPLSLAEVEVFRESDGKQSEEDIDKITEDKVVSTN
                     KVATQSSTNYEGVAALAVDGNKDGDYGHHSVTHTKADSNAWWQVDLGEEFTVSKVDIY
                     NRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDKVVDGLLSLKVPSVGAKLVKIELKSAA
                     IPLSLAEVEAYGSKRTPKKLSNIALTKETRQSSTDYNGFSRLAVDGNKNGDYGHHSVT
                     HTKGDSPSWWEIDLAQTEELEKLIIYNRTDAEIQRLSNFDIIIYDSNNHEVFKQHIDS
                     LESNNLSIDLKGLKGKKVRISLRNAGIPLSLAEVEVYTYK"
     gene            1704..3470
                     /locus_tag="VQ651_001676"
     CDS             1704..3470
                     /locus_tag="VQ651_001676"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HET2754639.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGCAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDSGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            3616..4767
                     /locus_tag="VQ651_001677"
     CDS             3616..4767
                     /locus_tag="VQ651_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET2754640.1"
                     /translation="MAIFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHRCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPQALTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIEHENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIVEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            4912..5796
                     /locus_tag="VQ651_001678"
     CDS             4912..5796
                     /locus_tag="VQ651_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET2754641.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
BASE COUNT         1868 a          970 c         1287 g         1674 t
ORIGIN      
        1 aattacagga cagataagac agctatgtgg gcattatcag gaaattttga tgcatcaaaa
       61 agttggaaga aagaagaatt agagttagcg aactggataa gcaaaaatta ttccatcaat
      121 cctgtagata atgactttag gacaacaaca cttacattaa aagggcatac tggtcataaa
      181 cctcagataa atataagtgg cgataaaaat cattatactt atacagaaaa ttgggatgag
      241 aatacccatg tttataccat tacggttaat cataatggaa tggtagagat gtctataaat
      301 actgaaggga caggcccagt ccctttccca acaccagata aatttaatga tggtaatttg
      361 aatatagcat atgcaaaacc aacaacacaa agttctgtag attacaatgg agaccctaat
      421 agagctgtgg atggtaacag aaatggtaat tttaactctg gttcggtaac acacactagg
      481 gcagataatc cctcttggtg ggaagtcgat ttgaaaaaaa tggataaagt tgggcttgtt
      541 aaaatttata atcgcacaga tgctgagact caacgtctat ctaattttga tgtgattcta
      601 tatgacaata atagaaacga agttgctaag aaacatgtta ataatttgtc gggtgaatct
      661 gttagtctag atttcaaaga aaaaggagca aggtatatta aagttaaatt actaacgagt
      721 ggagtgcctt tgagtttagc agaagtagag gtttttagag aatcagatgg taagcaatct
      781 gaagaggata tagataaaat aacagaagat aaagtagtct ctacaaataa ggtagctact
      841 caaagttcaa ccaattatga gggtgtagct gctttagcag ttgatggtaa taaagatgga
      901 gattacggac atcattcggt gactcatact aaggcagatt ctaacgcttg gtggcaggtc
      961 gatctgggag aagagtttac ggtttctaaa gttgatattt ataatagaac agatgccgaa
     1021 cctcagcgtt tatctaattt tgatgttatt tttctatctt catcaggaga agaagttttt
     1081 agaagacatt ttgataaagt agttgatggt ttgttatctt taaaagtacc ttctgtaggg
     1141 gctaagctag tcaaaataga attaaaatca gcagctattc cgttaagttt agcggaagtt
     1201 gaagcctatg gttcaaagag aactccgaag aaactttcta atattgcatt aaccaaagaa
     1261 actcgacaga gttcaacgga ttacaatggt ttttctcgtc tagcagttga tggaaataaa
     1321 aacggagatt atggtcatca ttcagtgact cataccaaag gagattctcc ttcatggtgg
     1381 gagatagatt tagcacaaac cgaagaatta gaaaagttaa ttatttataa tagaacagat
     1441 gctgaaattc agagattatc aaattttgat attattatat atgattcaaa taatcatgaa
     1501 gtttttaaac aacatattga cagtttagaa agcaataatc tatccataga cttaaaaggg
     1561 ctgaagggaa aaaaggttag aatttctttg agaaacgcag gaattccttt aagtttggca
     1621 gaggtagagg tttatactta taagtaattt taaaaattat catcggtagt atgaaagaaa
     1681 cagaaaaata agaggaaaat agtatgattc aacatccacg tattgggatt cgtccgacta
     1741 ttgatggtcg tcgtcaaggt gtacgcgaat cacttgaagt acaaacaatg aacatggcta
     1801 aaagtgtggc agatttgatt tcaagcacat tgaaatatcc agatggggaa cctgtggaat
     1861 gtgtgatttc tccatctacc attggtcgtg ttccagaggc tgcagcttcc catgagttgt
     1921 ttaaaaaatc aaatgtttgc gcaacaatta cagttacacc atgctggtgt tatggtagtg
     1981 aaactatgga tatgtctcca gatattcctc atgctatttg gggatttaat gggacagaac
     2041 gcccaggagc tgtctatctt gcagctgtac tagcttcaca tgctcaaaaa gggattccag
     2101 cctttgggat ttatggtaga gatgttcagg aagctaatga tacagatatt ccagaagatg
     2161 tcaaagaaaa acttttacgt tatgcgcgtg cagctcttgc aactggcttg atgagagaca
     2221 ctgcttacct atcaatgggt agtgtttcga tggggattgg cggttctatt gtaaatccag
     2281 atttcttcca agaatactta ggaatgcgaa atgaatcggt agatatgacg gagttcacgc
     2341 gccgtatgga ccgtggtatt tatgacccag aagagttcga acgtgctatg gtttgggtaa
     2401 aagaacatat caaagaaggg gttgaccgaa atcgtgagga tttgattctt tctaaagaag
     2461 agaaagaaaa acaatgggaa tttgttatta agatgttcat gattggacgt gacttaatgg
     2521 ttggtaaccc aaggcttgct gaacttggtt ttgaggaaga agcggttggt caccatgctt
     2581 tagtagctgg tttccaaggt caacgtcagt ggacagacca ttttccaaat ggagatttta
     2641 tggaaacttt cctcaatact cagtttgact ggaatggtat tcgaaaacca tttgtatttg
     2701 cgacagagaa tgattcacta aatggtgtgt ctatgctctt taattatcta ttaacaaata
     2761 ctccacaaat ctttgctgat gtgcgcactt attggagtcc agaggctgtt aaacgtgtaa
     2821 cgggacatac tttagagggt tgtgctgcag ctggcttctt acatctaatc aactctggtt
     2881 cttgtacatt ggatggtaca ggtcaagcta ctcgagatgg caaacctgtt atgaaaccat
     2941 tctgggagtt ggaagaaagt gaagtacagg ctatgcttga aaatacagac ttcccaccag
     3001 caaaccgcga gtacttccgt ggaggaggat tctcaactcg tttcttgacg aagggggata
     3061 tgccagtaac aatggtacgt ctcaatcttc taaaaggggt tggtccagtg ctacaaattg
     3121 cagaaggtta cacacttgaa cttcctgaag atgttcacca tactttagat aatcgtacag
     3181 attcaggatg gccaactact tggtttgctc cacgtttgac aggaaaaggt gctttcaagt
     3241 ctgtctatga cgtcatgaat aattggggag ctaatcacgg agccataaca tatggacaca
     3301 ttggagcaga cttgattacc ttggcttcta tgttgagaat tcctgtcaat atgcataatg
     3361 tacctgagga agatatcttt agacctaaaa attggtcctt atttggaaca gaagatctag
     3421 aatcagcaga ctatcgtgca tgtcagttgt tgggaccact acataaataa aacttgttta
     3481 tataggaggt gaacttacgt ccctcctatc cttttaaaaa gatttgttaa acaattcaca
     3541 aataattgaa aacgaataca aaaagtaata taatgatgtt aaatagatag cgcggaggcg
     3601 caggaggaaa attatatggc tatattttat gttccggcag tcaaccttat tggaaaaggt
     3661 gttgtaaatg aagtgggtcc ttatatcaag gaacttggct ataaaaaggc acttttggtg
     3721 acagataagt acatcgaagg cagtgatatt ttacctaaga ctttaaaacc actggataca
     3781 gaaggaatcg aatatgtcat ctttagcgat gtagagccaa accctacttg taaaaatgtc
     3841 acagatgggg tagctgcttt gcaagaacat agatgtgatt ttatcatcag tcttggcggg
     3901 ggttctccac aggatgcagc tagttgtatt tctatcatgg ctacaaatgg tggaaaacca
     3961 caggattatg aagggcttca taagtctgct aaaaaaggct tgccggttgt ggctatcaat
     4021 acaacggcag gtacatcagc agaaatcact attaactatg tgattactga tgaagaacgc
     4081 aaggttaaga tggtaatggt tgacaagaat agccttgcct tgatttctgt caatgaccct
     4141 gaactcatgc tttccaaacc tcaagccttg actgctgcta ctggtatgga tgctctgact
     4201 cacgctgttg aagctttggt aacacctggt gcttatgatg taaccaagaa actgtctatt
     4261 ggtgctattg agcttatcaa ggaatatctt cctcgtgctg tagaaaatgg acatgatatt
     4321 gaagcgcgtg aaggtatggt caatgccatc ttccttggtg gtatgagctt taataatgct
     4381 ggtcttggtt atgttcactc aatggctcac caactcggtg cagtatataa tttgccacat
     4441 ggtgtttgct gtgccatgtt gctaccagtt atagaacatg aaaatgctaa acgtgtacca
     4501 gaagctttcc gcaatgttgc caaagccttg ggacttcatg tagaaggtaa atcagatcaa
     4561 gaatgtgccg attatgcgat tgttgagatt gagaagcttt ctgagacagt aggtattcct
     4621 aagaaactga ctgaacttgg tattgaagaa aaagatttcg actttgaata cctttctaag
     4681 aatgccttga ttgatgcctg cgcaccagga aatccattta tgccaacctt agaagaaacg
     4741 attgcctttt ataaagagtt attttagcaa gagattggac gacaatagga caattcacct
     4801 ctaattattg acatttcagg aaaaatcgca gtaaaaacgg cacagccttc tttaaatgtg
     4861 ctataatact agaaaaatac ttgtggaggt tccattatgg caatattttt catgattttt
     4921 ctgattgttt gtgtgctcct attggtgata gtcacactga gtacagttta tgtggttcgt
     4981 cagcagtcgg tggcgattat tgaacgcttt gggaaatacc aaaaggttgc taatagcggt
     5041 attcatattc gcttgccttt tgggattgac tcgattgcag cacggattca gttgcgcttg
     5101 ttgcaaagtg atattgtggt tgagactaag accaaggaca atgtgttcgt tatgatgaat
     5161 gtagcgactc agtaccgtgt caacgagcag agcgtgacag atgcttacta taaactcata
     5221 cgtccagaat ctcagattaa atcttatatc gaagatgctc ttcgctcttc tgttccaaaa
     5281 ttaaccttgg atgaattgtt tgagaaaaaa gatgagattg cccttgaagt tcaacaccaa
     5341 gtagcagaag aaatgaccac ttacggctac attatcgtga aaaccttgat taccaaagtc
     5401 gaaccggatg cagaagttaa gcaatccatg aatgaaatca atgcggcgca acgtaagcgg
     5461 gtcgcagcac aagaattggc ggaagctgac aagattaaaa ttgtcactgc agctgaagca
     5521 gaagcagaaa aagaccgcct tcatggtgtg gggattgccc aacaacgtaa ggcgattgtg
     5581 gatggattgg cagagtctat caccgaactc aaggaagcca atgttggcat gacagaagaa
     5641 caaatcatgt ctatcctctt gaccaaccag tatttggata ccttgaatac ctttgcctct
     5701 aaaggaaatc aaaccatctt tttaccaaat acgccaaatg gtgtggatga tatccgaaca
     5761 caaatcttgt cagcccttcg cgctgagaag aaataatag
//
LOCUS       DAVBHS010000123         5789 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVBHS010000123 DAVBHS010000000
VERSION     DAVBHS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.123"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            219..767
                     /locus_tag="VQ651_001679"
     CDS             219..767
                     /locus_tag="VQ651_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2754642.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            complement(1004..>5789)
                     /locus_tag="VQ651_001680"
     CDS             complement(1004..>5789)
                     /locus_tag="VQ651_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2754643.1"
                     /translation="QGERGLTGAKGEKGDQGERGLTGAQGEKGDRGERGLTGAKGEKG
                     AQGQAGRDGVTPTVTVKDNKNDGTHTITINDGRGNVTSTVVRDGFDGASPLVATQRND
                     ADKTTTVIFYYDKNGNNELDASDKKLKEVVIADGAKGEKGDKGEQGLQGRDGEQGPKG
                     EDGKTPTVKVTDGQDGTHTITINDGKGGITTTVVRDGFDGASPLVSTHRNEADKTTTV
                     IFYYDLNDNNQFDEGDTKLKEVVIADGKQGPKGDKGDNGKDGFTPEVTVTDNNNGTHT
                     ITITQPDNRPSLTTIVKNGEDGKTPKVKAERDDAKKQTTLTFYIDKDGDGSYTAGKDE
                     LVQTTVVKDGQDGAAGASGRDGKEVLNGKVDPTTEGKDGDTFVNTQTGDVFVKKGNTW
                     EPAGNIKGPKGDKGADGAKGEKGAQGERGLTGAQGVKGEKGDQGERGLTGSKGEKGDQ
                     GERGLTGAQGAKGDKGEQGLQGRDGAQGPKGADGQRGPAGPQGPKGEQGNPGTPGKDG
                     KSLIAVKNGTETKVYVEDPARPGQPLNPNQPLATITDGRNGTNGQSPTITATRSVQNG
                     KNGVLVTITPVGGRPQTTFVEDGQKGADGKTPTVTITEGQNGTHTLTVHNPGSPDVTT
                     TIRDGATGQAGRDGKDVLNGKVNPQPNQGKNGDKYINTETGDVYVKNNGNWDKEGNIK
                     GPKGDKGADGAKGEKGDQGERGLTGAQGAKGADGAVGRDGRDGKDVLNGKANPEAHQG
                     KDGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGERGEDGKTPEVTVTPGKDGHST
                     DITFTVPGKDPVTVNVKDGENGLNGKTPKVDLLRVQGKNGNPSHTIVTFYTDENNDGK
                     YTPGTDELLGSEMIKDGAKGADGRDGKSLLTVKDGKETKVYQEDPANPGQPLNPEKPL
                     AVIRDGVDGKSPTVTAVRKDEAGHKGVEITVDNHDGSQPTTVFVQDGAKGETGATGQD
                     GQTPTITTQRGQDGQSTVVTITTSGKDPVTFTVKDGKNGKDGRAPKIKVEDITSPSRI
                     RRDTDAAATPTRNGIRVTVYDDVNDNGVYDEGVDKVLNSKDIYNGIDGRDGSAPTITT
                     KDNGDGTHTITVQNPDGSESTTVVKDGKDGKTANITTTENPDGSHTITVTNPDGSTKE
                     TVVKNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNVTNAIIKDGKDGKAATATTTENPD
                     GSHTVTITNPDGTKNEFVVKNGRDGVDGRTPTASVRDNGDGSHTIVITNPEGVTTETT
                     VRDGKSPKVTITDEQNGTHKISVLNGDGTTTTTTVRDGREPKLEVIDNNDGSHTIKVT
                     GADGKGTTTTIFDGKSPKANIVDNGDGTHTLTIVDSDGREYKSIIKDGKDGKDSVSPT
                     VTVKNNNDGTHVVTITNPDGSKTEMVIKDGKDGKSPKVSVEDNGDGSHTITIINSDGT
                     VTKTVIKDGRDGRDGRDGKDGKDGKDGKCGCQDKPVTPSNDKPVPPTPNVPTPEVPVK
                     PVPAQPTPNVPTPEVPVQPTPAVPTPEVPVKPVPAVPEQPVVPTPAQPATPVNANPVA
                     PTTGKENRGDKLPETGSQSDYISVLLGSGILLSLYVGRRKEA"
BASE COUNT         1246 a         1341 c         1048 g         2154 t
ORIGIN      
        1 gtgctttgag caaccaattc tatttctccc ttcaaacaaa accgattttg aaagtgaaac
       61 agttcttact ttttcagtca caaatgacta gagtttccga aattttacaa ttttcaaata
      121 aactctgagc aaatttcttg caagttcttt tgttttgttg taatgtattt tataacaacg
      181 agagagttct caaaatttga agaaaaagga gacacatcat gtctaaaaaa gtattattta
      241 tcgtcggatc actacgtcaa ggttctttca accaccaaat ggcgctcgaa gctgagaaag
      301 cacttgctgg taaagcggaa gttagctacc ttgattattc agcccttcct ctcttcagcc
      361 aagatttgga agttccaaca catccagctg tagctgctgc tcgtgaagca gttctcgttg
      421 cggatgctat ctggattttc tctccagtct acaacttctc tatccctggt acagtaaaaa
      481 acttgcttga ctggctatct cgtgcccttg acttgtctga tacgcgtggc gtttctgccc
      541 ttcaagacaa gtttgtcacc gtatcatctg tagccaatgc agggcacgat caacttttcg
      601 ctatctacaa agacctcttg ccatttatcc gtacacaagg cgttggtgat ttcactgctg
      661 cacgtgttaa tgactctgcc tgggcagacg gaaaattggt tcttgaagaa acagtcctaa
      721 actcacttga aaaacaagct caagacttgg tcgaagcgat tcagtaaaaa atagaaaatt
      781 ttagaaagtc taaatcaatt atagccacct aatagggtgg ctttttattt ctcaatcaag
      841 attttcagac aaacatcagc catccaaaaa aaccttgagc gttaccactc aaggttttta
      901 gcagtctttt ccatcatagg aaaatcattc taagaatatt ctttcattcg agatctatca
      961 cttatcatct tctttcgaga aattagtgac tgcttttggc tgattaagct tcttttcttc
     1021 gtcctacata taggctcaat agaataccgc tacctaaaag aacagagata taatcagatt
     1081 ggcttccagt ttcaggtaat ttgtccccac ggttttcttt acctgtagtt ggtgctactg
     1141 ggttagcatt tactggagtt gctggttgag ccggtgttgg tactactggt tgttctggaa
     1201 ccgctggtac tggtttaacc ggtacttctg gtgttggaac agctggagtt ggttgtactg
     1261 gcacttctgg tgtcggtaca tttggtgttg gttgcgctgg cacaggttta accggtactt
     1321 ctggtgtcgg cacatttggt gttggaggaa ctggtttgtc atttgatggt gttactggtt
     1381 tgtcttggca tccacatttt ccatctttac cgtctttacc gtctttgccg tctcgaccat
     1441 cacgtccatc tctaccatct ttaataactg tttttgtcac agttccatca gaattgatga
     1501 ttgtgattgt atgactacca tcaccattat cttcaacaga aacttttggt gatttaccat
     1561 ctttaccgtc tttaatcacc atttctgtct tacttccatc tggattagtg attgtaacaa
     1621 cgtgagttcc atcgttatta tttttaacag ttacagttgg tgaaacgcta tctttgccgt
     1681 ctttaccatc tttgataata gatttgtatt cacgaccatc agaatctacg attgttaatg
     1741 tatgagttcc atctccgtta tcaacgatgt tcgcttttgg tgatttacca tcaaagattg
     1801 tagttgtcgt tcctttacca tcagcacctg tcactttaat agtgtgtgaa ccatcgttgt
     1861 tatcaataac ttcaagtttt ggttctctac catcacgaac tgtagttgtt gtagttgttc
     1921 cgtcaccatt tagaacagag atcttatgag ttccattttg ttcatcagtt atagtcactt
     1981 ttggtgattt accatcacga actgtggttt cagttgtcac accttctgga tttgtaataa
     2041 cgattgtatg acttccgtct ccattatcac gaacagatgc ggttggagta cgtccgtcaa
     2101 caccgtcacg tccattctta acaacaaact cattcttagt tccgtctggg ttagtgattg
     2161 ttactgtgtg gcttccatct ggattttcag tagttgttgc tgttgcagct ttaccgtctt
     2221 taccatcttt gatgatagcg ttggtaacat tgccgtctcc atctgtaact ttaacagtat
     2281 gagttccatc gttgttatcc gttacttcaa ctttaggagt cttaccgtct ttaccgtttt
     2341 taacaacagt ttctttagtt gaaccatctg gatttgtcac cgtaattgtg tggcttccat
     2401 ctgggttttc tgttgtagtg atattcgcag ttttaccgtc tttaccatct ttaacaactg
     2461 ttgttgattc agaaccatct gggttttgaa ctgtgatagt gtgagttcca tctccattat
     2521 cttttgtagt aatagttgga gctgaaccat cacgtccatc tataccgtta taaatatctt
     2581 tactatttaa tactttatcg acaccttcgt cgtatacccc attgtcatta acatcatcat
     2641 aaactgtaac acggataccg ttacgcgttg gagttgcagc agcatctgta tcgcgtctaa
     2701 ttcttgaagg tgaagtaata tcttctactt tgattttcgg tgcacggcca tctttaccat
     2761 tcttaccatc ttttacagtg aaggttactg gatctttacc tgatgttgtg atagttacaa
     2821 cagtgctttg gccatcttgt ccacgttgag tagtgattgt aggagtttgt ccatcctgac
     2881 cggttgcacc agtttctccc ttagcaccat cttgaacaaa gactgtagtt ggttgtgaac
     2941 catcatggtt atcaacagtg atttctacac ctttatgccc tgcttcatct ttacgaacag
     3001 ctgtaactgt aggtgatttt ccatctactc catctctaat taccgcaagt ggtttttctg
     3061 gatttaatgg ttgtcctggg ttagctggat cttcttggta aactttagtt tctttaccat
     3121 ccttgacagt aagcaatgat ttaccatctc gtccgtccgc gcctttagca ccatctttaa
     3181 tcatttctga acctagaagt tcatcagttc ctggtgtata tttgccgtca ttgttttcat
     3241 ctgtatagaa tgtcacaatt gtatgagatg gatttccgtt ttttccttgg acacgaagta
     3301 aatcaacttt tggagtttta ccgttcagac cattttctcc gtccttaaca tttactgtaa
     3361 ctggatcttt acctggaaca gtgaatgtaa tgtcagtact atggccatct ttacctggag
     3421 ttacagttac ttctggagtc ttaccatctt ctccgcgttc acctttgtca cccttagggc
     3481 ctttgatgtt gccctcttta tcccagttgc cgttattctt aacgaagacg tcgcctgttt
     3541 ctgtattaac gtatttatcg ccgtctttac cttgatgtgc ttctgggtta gctttgccgt
     3601 tcaacacgtc tttaccgtca cgtccatcac gacctactgc gccatccgca cctttagctc
     3661 cttgcgctcc agttaggccg cgttcgcctt ggtctccttt ttcgccttta gcaccatctg
     3721 cacctttgtc ccctttaggg cctttgatgt tgccttcttt atcccagttt ccattgtttt
     3781 taacatagac atcaccggtt tcggtattaa tatatttatc tccattttta ccttggtttg
     3841 gttgtggatt tacttttccg tttaatacat ctttaccatc acgacctgct tgtcctgtag
     3901 ctccatcacg gatcgtagtt gtcacatctg gacttcctgg attatgaacc gttaatgtat
     3961 gtgtgccgtt ttgcccctca gttattgtta ctgttggggt tttcccatca gcaccctttt
     4021 gtccatcctc tacaaatgtt gtttgtggac gacctcctac tggtgtaatt gttactaata
     4081 caccattttt gccattttgc acacttcgtg tcgctgtaat tgttggtgat tgtccattag
     4141 taccgtttct tccatcagtg atagttgcta atggttggtt tggattaagt ggttgtcctg
     4201 gtctagccgg atcctctaca tagacttttg tttcagttcc atttttaaca gcaattagag
     4261 attttccatc tttacctgga gttcctggat taccttgttc tccttttggt ccttgtggtc
     4321 ccgctggtcc tctttgtcca tctgcgcctt ttggtccttg agctccatca cgaccttgaa
     4381 gaccttgttc acctttgtca cctttggcac cttgcgctcc agttaaaccg cgttcacctt
     4441 ggtctccttt ttcaccttta gatccagtta gaccgcgttc accctgatct cccttttcac
     4501 ccttgacacc ttgcgctcca gtcaggccgc gttctccttg ggctcctttt tcacccttgg
     4561 caccatctgc acctttgtca cctttcggtc ctttgatgtt tcctgctggt tcccaagtgt
     4621 tacctttctt aacgaataca tctcctgttt gtgtatttac gaatgtgtct ccatctttac
     4681 cttctgttgt tgggtctact tttccgttta atacttcttt tccatcacgt ccagatgctc
     4741 ctgcagctcc gtcttgtccg tctttaacta ctgttgtttg aactaactcg tcttttcctg
     4801 ctgtgtaact tccatctcca tctttgtcaa tgtagaatgt taatgttgtt tgtttcttcg
     4861 catcatctcg ttctgctttg acttttggtg tttttccatc ttcaccgttt ttaacgattg
     4921 ttgttaatga tggtctgttg tctggttgtg tgattgtgat ggtgtgtgtt ccgttattgt
     4981 tatctgtaac tgttacttct ggtgtgaatc catcttttcc gttatcacct ttgtcacctt
     5041 ttggtccttg ttttccatct gcgataacaa cttctttaag ttttgtatct ccttcatcaa
     5101 attgattatt atcatttaga tcataataga aaataacagt tgttgtttta tctgcttcat
     5161 ttctatgagt agaaacaaga gggcttgcac catcaaatcc atctcttact actgtagtag
     5221 ttataccacc tttaccatcg ttaattgtaa ttgtatgcgt tccatcttga ccatcagtta
     5281 ctttaactgt tggggttttc ccatcttctc cttttggtcc ttgttcacca tcacgccctt
     5341 gaagaccttg ttcacctttg tccccttttt cacctttagc accatctgca attacaactt
     5401 cttttaattt cttatcagaa gcatctaatt cattgtttcc atttttatcg taatagaaga
     5461 taacagttgt tgtcttatct gcgtcatttc gttgagtcgc aactaatgga cttgcgccat
     5521 cgaaaccatc tcttacaaca gtacttgtaa cattacctct accgtcgtta atagtgatag
     5581 tatgagtgcc gtcattttta ttatctttaa cggttacggt tggagttaca ccgtcacgac
     5641 ctgcctgacc ttgggctcct ttttcacctt tagctccagt taaaccacgt tcgcctcggt
     5701 ctcctttttc accttgggct ccagtgagac cgcgttcacc ttggtcccct ttttcacctt
     5761 tagctccagt taaaccgcgt tcaccttgg
//
LOCUS       DAVBHS010000124         5769 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVBHS010000124 DAVBHS010000000
VERSION     DAVBHS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.124"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            120..1454
                     /gene="pepC"
                     /locus_tag="VQ651_001681"
     CDS             120..1454
                     /gene="pepC"
                     /locus_tag="VQ651_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HET2754644.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALECRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(1510..2421)
                     /locus_tag="VQ651_001682"
     CDS             complement(1510..2421)
                     /locus_tag="VQ651_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2754645.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(2445..3248)
                     /locus_tag="VQ651_001683"
     CDS             complement(2445..3248)
                     /locus_tag="VQ651_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HET2754646.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(3276..4265)
                     /gene="manX"
                     /locus_tag="VQ651_001684"
     CDS             complement(3276..4265)
                     /gene="manX"
                     /locus_tag="VQ651_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HET2754647.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(4498..5517)
                     /gene="adhP"
                     /locus_tag="VQ651_001685"
     CDS             complement(4498..5517)
                     /gene="adhP"
                     /locus_tag="VQ651_001685"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HET2754648.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLTLAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         1725 a         1225 c         1225 g         1594 t
ORIGIN      
        1 gattttcgaa gagtataaat tcgaaaaagg tgagggacaa tgtcctcgcc ttttatgttt
       61 tttagttgct tcctttgtga aaagagttat aatagactgt agaataaaag gaggaatcta
      121 tgaacgcgat tcaagaatca tttactgata aactatttgc caactatgaa gcaaatgtaa
      181 aataccaagc gattgaaaat gctgccagcc acaacggaat ttttgcagct ctggaatgtc
      241 gccaaagcca tgtagacaac acacctgttt tctcattgga tttaaccaag gacaaggtca
      301 ctaaccagaa agcgtctggt cgttgctgga tgtttgcggc tctcaacacc ttccgccaca
      361 aactcatctc gcaatacaaa ttggagaact ttgagttgtc acaagcccac actttcttct
      421 gggataagta tgagaaatca aactggttct tggagcaagt cattgcgact tcagaccaag
      481 aattgactag ccgcaaggtt agcttcttac tccaaacacc tcaacaagat ggcggtcaat
      541 gggatatggt cgtttccctc tttgaaaaat acggtgtcgt gcctaagtca gtttatcctg
      601 agtctgtttc atctagcagc agtcgtgagc taaatgcgat ccttaataaa ttgcttcgtc
      661 aagatgctca aatcttgcgt gatttgcttg tttctggtgc agatcaagcg actgttcaag
      721 ctaagaaaga agacctcttg caagaaatct ttaactttct tgctatgtca ttaggacttc
      781 caccacgcaa gtttgacttt gcttatcgcg ataaagataa caactataaa agtgaaaaag
      841 gaatcacacc acaagagttt tacaagaaat atgtcaatct tcctttagaa gactacgttt
      901 ctgttatcaa tgctccaact gctgataaac cttacggaaa atcttacaca gttgagatgt
      961 tggggaatgt ggttggtagc cgtgcagttc gctacatcaa cgttccaatg gagcgcttga
     1021 aagaattggc gattgcccaa atgcaagcag gtgagactgt ttggtttggt tctgatgtcg
     1081 gccagctcag caaccgtaag gctggcatcc ttgcgacaga tgtttatgac tttgaatcaa
     1141 gcatggacat taaacttact caagacaagg ctggacgttt ggactatagc gaaagcttga
     1201 tgacccacgc catggtcttg acaggtgttg acttggacga aaatggtaaa tcaaccaagt
     1261 ggaaggttga aaactcatgg ggagacaagg tcggtacaga tggttacttt gttgcctcag
     1321 acgcttggat ggacgaatac acataccaaa tcgttgttcg taaggaattg ctgacagcag
     1381 aagaacaagc tgcctatgga gcagaaccaa tcgtacttgc accatgggat ccaatgggag
     1441 ccttggctga ataaaagcat agaaaaaagg aatcagattt tagaacctgg ttccttttta
     1501 gttgcttgat tacatgatgt gaagaacatg tgccacaata cccactgcga agagggcaag
     1561 gataatagtg attggagata cttttttctt aagtaagtac atgcaaagta aagtaaggag
     1621 tagtcctgat aatccaggaa tcaacatatc caagttttgt tggaaagtag taactttttc
     1681 aggagtttga gacaatcctt gtcctacttg tgcgaatgct tcttggatac ctttagaccc
     1741 ttctggcaat ttatcccaat ggatataagc cttttcatct agttgaactt tagaaacatc
     1801 gaaagcaaat ttaatattta cccagcgttg aacaaggaca gcaagaatga acatcccaag
     1861 gatagaagct cctttagtga tatcttgaag gataccacca gacatatctt tagtgatttc
     1921 tgatccagcc ttgtatccaa tctcttgaac ataccacaag aatgacatac gaatcaagtt
     1981 ccatgcaaca aagaagagga gtggccccaa gatattgcca gtaagggcaa gtgaagcacc
     2041 gagagatcca aggattgggc gtactgtaaa ccagaatact gggtcaccga taccagcaag
     2101 aggtcccatc ataccgattt taaccccttg gatagcagcg tcatcgattt ccacaccgtt
     2161 agcacgttct tcttcaagcg caagagtaac ccccatgact ggagcagcta cgtatggatg
     2221 agtgttgaag aactcaaggt gacgctcaag agcagcgatt tgatcttctt ttttagtata
     2281 gagttttttg atagctggaa tgagtgtata agcccagccc aagttttgca tccgttcaaa
     2341 gttccaagac ccttgtaaga aggttgaacg ccaccaaact tttttacgat ctgatttagt
     2401 taattgaagt ttttcagtca tgatgttttc agtcctttct tatcttagta gtcttctagg
     2461 atatcgccga ttgggtcgtt agaagttgcg gctcctccgc caccatttcc accagtttta
     2521 gaaaggtgaa ggtagataag agcgatagca acaccgatag caccgaatcc gattagagta
     2581 atatctgaca cagcagcaag aacgaaacca agagcgaaga atggccatac ttcacgagtt
     2641 gccatcatgt tgataaccat ggcgtaacca acggcaacga ccataccacc accgatagcc
     2701 ataccatctt tgagccagtc tggcatggca ctaaggatac tttgtacagt ttcagttggt
     2761 accataagga gaagagctgc aggaagcgcg atacgaagtc cttggaaaag tagcgcaatg
     2821 aaatgagcac gctccacagc gccgaagtca ccttttttag cggcagcatc agcagtgtga
     2881 accaatccaa ctgaaattgt acgaacaatc atagttaaga atagaccagc tacggcaaga
     2941 gggatagcaa ccgcttgggc aacaccgata ccagtcttgg taaagtcacc accaagaacc
     3001 atgataatgg cagcagcgac agaagcaagt gcagcatcag gagcgatagc agcaccgata
     3061 tttgaccaac caagggcaat catttgaagc gatccaccga ggataatccc tgcttccaag
     3121 tgacctgtta caagcccaat aagggtacag gctacaagtg gttggtgaaa ttggaactgg
     3181 tcgaggatgc cttcaagacc tgcaaagaag gctacaacga ctactaaaac catagaaata
     3241 atagacatgt ttaaaatcct ttcataaata atggcttatt tgacattggc tttgttaatc
     3301 aagtcaaaca aatctttttt agaatcattt ggtactttac ggacatcaaa ttcaacaccc
     3361 aagtcacgca ttttttcaaa tgtagcaaca tcttctttgt ccatagacaa aacggtattg
     3421 accaatgttt tacctgttga gtgagccata gaaccaacgt taagagtctt gattggcacg
     3481 ccgccttcga tggcacgaag ggcatcttga ggtgtttcaa acaagataag ggcatgtgtt
     3541 tctccaaaac gtgggtcttt tgaaatctca atcagttttt gaattggaac cacgttagcc
     3601 ttgacattac ctggagctgc ttgtttaatc aattctttac gaaggtcgtc tttagccacg
     3661 ttatctgaag caacgatgat acgatttgct tttgaatctg gagtccaagc agttgcaacc
     3721 tgaccgtgaa gtagacgtgt gtcaagacgg gcaagattga ttttcaattt accgtctccg
     3781 ataacagttc cttctgggat agcagtttgg gcaactggag cagctgcagc gcttgcaact
     3841 tcttcaactg gatttagctc ttctggaaga gctttgatgc catctttggc ttctttaatg
     3901 atattagcag cgactttttc tacacctgca gcagcgtcca tgaggcgctc tgtgtaggct
     3961 tgaatcaaca tcggtaagtt aagtcctgtg atgatggcaa acttacgctc aggattttct
     4021 cccatcacgc gactagcttg gttaaatgga gaaccactcc aaaggtcagc caaaactaga
     4081 acctcatctt ctgcgtcaaa tgcagcaaca gcgttattaa acttagcgta tagatcatca
     4141 ggaccttcat ttggcataaa ggtcacaact tgaacctttt cttgttcacc aaagatcata
     4201 gatcctgact gatgaatacc cgcagcaaat tcgccgtggc tcgcaataat gattccgata
     4261 ctcattattg tcattcctcc ttttttgttt tagttttcat ttaagaaaac tttaagactg
     4321 tttaagtata aaccgttttc ataaaaaaag caactattta tcataaaaaa ttaaaaaatt
     4381 tttatttttg tatgttgata tgttgagttt ttgaggagta atcttgaatt cttatttaaa
     4441 atttaatata aaaaagttgg aagaagcttc caacttttaa aataggtttc tattaaatta
     4501 gtgggtgaag tcgagtacca tacgtccttg gatttggcct ttttccattt cgtcgaaaat
     4561 ggcaacagca tcttctactg gacgtttttg aacaactggg actaccagac cttctgcacc
     4621 aaattggaag gcttcttcta agtctttacg agttccgaca agagaaccga tgacttggat
     4681 tccatcgagg actgttttaa cgatgctgag ttccatcatt tcagaaggaa gaccaacagc
     4741 gacgacgcga ccaccagcac gaatggagtc aacagcctgg ttgaaggcaa ctttagacac
     4801 agcagttacg acagctgaat gagctcctcc atcagttttt tctttaatga gtccagctac
     4861 atcttcaact tcgaggccgt taatcacaat gtcagcgcct acttcttttg caagggtaag
     4921 tttgtcattg ttgatatcga ctgcgataac atgagcattg aatacttttt tagcgtattg
     4981 aacagcgagg ttaccaagtc caccagcacc gtaaagaaca acccattggc ctggttcaac
     5041 ttttgcttct ttgatagctt tataggttgt tactccagca catgtgatag aagaagcttg
     5101 ggctggatca agtccgtcag gaactttgac agcatagtca gcagttacga tacattgttc
     5161 agccatacca ccgtctactg agtagccagc atttttcact gtacggcaaa gggtttcgcg
     5221 accagttgta cagtattcgc aagtgccaca tccttcaaag aaccaagcaa cgctgacgcg
     5281 gtcaccgact ttaaggcttt tcacatctgg agcaatctct ttaacgatac cgataccttc
     5341 gtgcccaaga acacgtcctg ggacttgacc aaagtcacca tgagcaacgt ggaggtcggt
     5401 gtggcaaacg ccacagtatt caacttctac aagtgcttcc ccagtttcaa gtggacggag
     5461 tactttttct tcaatagcaa caccagtgct ttctggattt acaacaacag ctttcatagc
     5521 tatcctcctt gatattgacc gagaacaggg acaacgggac tggattgatt ttttgtcaac
     5581 agagtcgagt gtgacgagct ctcttgtatt tatatgtgaa gtatatcaca aaaggaagcc
     5641 ttttccaagg ttttggagac aaaaagtaga acaatcgatt aactggttga tgagactgtg
     5701 aaaagagcac aaagaccagc ttgcaagctg gtcatactct tcgaaaatct cttcaaacca
     5761 cgtcagctt
//
LOCUS       DAVBHS010000125         5761 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVBHS010000125 DAVBHS010000000
VERSION     DAVBHS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.125"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(52..1113)
                     /locus_tag="VQ651_001686"
     CDS             complement(52..1113)
                     /locus_tag="VQ651_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET2754649.1"
                     /translation="MNKRVQAFLAKMQEKELDGVIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            complement(1106..3937)
                     /gene="uvrA"
                     /locus_tag="VQ651_001687"
     CDS             complement(1106..3937)
                     /gene="uvrA"
                     /locus_tag="VQ651_001687"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET2754650.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAIKASSVEQIVDKVLELPERQRLQILSPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDNSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPNASKTLHEGALAPWNPISSNYYPNMLEQAMTAFGVAMDKP
                     FEDLSEEDKNLILYGSDGKKFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCHGYRLNDQALSVRVGGEQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            4069..5013
                     /locus_tag="VQ651_001688"
     CDS             4069..5013
                     /locus_tag="VQ651_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET2754651.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            5025..5708
                     /locus_tag="VQ651_001689"
     CDS             5025..5708
                     /locus_tag="VQ651_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HET2754652.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASVFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
BASE COUNT         1598 a         1401 c         1170 g         1592 t
ORIGIN      
        1 aatttttttt gacttttttc gtcattttcg tacttttttc ttgacaaaca actaaatgac
       61 tatcaactct tttggagcta gggtcaataa ttcacaacct gtctctgtaa tcaggatatc
      121 atcctcgata cgaacgccat atttgccttc gatatagata cctggttcat cggtcaaggc
      181 catacctgtc ttaatagttt ctgtagaagt ctgactaaag tagggttcct catggatatc
      241 cagaccaata ccgtggccaa tgccgtgagt aaagtagtca ccataacctg cctcaatgat
      301 aatatcacga gggattttgt caaagtcacg gaaacctaag cctgccttag cttggtcaat
      361 caaggcttgg ttagctttta gaaccgtatt gtaaatctct gcctgctcat cgctaacatg
      421 ccctagatag atagtccggg tcatatcact gacatagtgg tcatagagac agccgaagtc
      481 catggtaatg gcttctccca actccactgg tttgtgcatt ggatgggcat ggggtttaga
      541 agaattgata ccgctagcta ggatcgtatc aaaagataag ccagatgctc ccaactcacg
      601 catgcggaaa tcaaggaagt tggcaatctc aatttcagtt tttcctggtt tgataaagtc
      661 aagcgcatcg cggaaagctt ggtctgagat agaacaagcc ttgcgaatcg ctgcaatctc
      721 tgcctcatcc ttaatcatac gaagaccttc cacaaactga gtttgtggaa gcaagttcaa
      781 acctgcaaaa gctgcctgca tacggtggta ataagacact gaaatctcat cttcaaaacc
      841 gatacgagtc aagcccatgt ccttaacaat tcctgcaatg acagccaatt catcacgatc
      901 agccacaatc tcaaaaccac tggtttcttg cttagctgcg atgatatagc gagagtctgt
      961 cactaatacc tgacggtcac ggctgataaa gactgttccg tttgagcccc aaaaaccagt
     1021 caaataatag acgtttttaa gattgttgat gatgacacca tctagttctt tttcttgcat
     1081 tttagctaga aatgcttgta cgcgtttatt catgatgtaa ctttcctttc aaatagtgtc
     1141 ctgtatagct ggcttcgttg gcagctactt cttctggagt tcctgttacg atgatggttc
     1201 caccaccgac accgccctca ggtcccaagt caatgatatg gtctgccgtc ttgataacat
     1261 ccagattgtg ctcgatgacg aggactgtat tgccatcgtc tacaaagcga gctaaaacct
     1321 tgagcaggcg agcaatgtcc tctgtatgaa gccctgtcgt cggttcatcc agaatgtaga
     1381 aagattttcc tgtcgagcgt ttgtggagtt cgctagctaa cttcatacgt tgggcttctc
     1441 ccccagaaag ggtggtagct ggctgtccca aggtcacata gcctagccct acatccttga
     1501 tggtctggag tttgcgttga attttcggaa tgtgttggaa aaattctacc gcatcgttga
     1561 ccgtcatatc caagacctgc gaaatattct tttccttgta gtgaacttct agggtttcac
     1621 tgttatagcg ggttccgtgg caaacttcac aagccacata aacatctggc aagaagtgca
     1681 tctcaatctt gataatcccg tcacctgagc aagcttcaca gcgacctccc ttgacgttga
     1741 aactgaagcg tcccttcttg tagcctcgaa tcttggcttc atttgtctga gcaaaaaggt
     1801 cacgtatatc gtcaaaaact cctgtatagg tagctgggtt agacctcggc gtccgtccga
     1861 tagggctctg gtcaatatca atcaaacggt cgacatgctc aatccctgta atagtcttaa
     1921 acttaccagg tttgtctgaa ttacggttga gcttctgggc aatggctttt ttgagaatgc
     1981 tgttgattag agtcgatttc cctgaacccg acacacctgt cactgcgata aattttccta
     2041 gtggaaagcg agccgtgaca ttttgcaagt tgttctcacg cgctcctatc acttcaataa
     2101 aacgaccatt tccgacacgg cgctcttctg gtactgggat gacacgtttg cctgacaagt
     2161 actgacctgt gatagacttg ctgttgcgag ccacttgctt aggtgtacct gctgcaacaa
     2221 tctcaccacc aaaaacaccg gcaccaggac caacgtcaat cagataatca gcttcacgca
     2281 tggtatcttc gtcgtgttcc accacgataa gagtattgcc caagtcacgc atctttttca
     2341 gactggcaat caggcggtca ttgtctctct ggtgaagacc gattgacggc tcgtctagga
     2401 tatagaggac gcctgatagg ttggaaccaa tctgggttgc caaacgaatg cgctgacttt
     2461 ccccacctga aagggttcct gctgaacgtg acagggttag atagttaaga cccacattat
     2521 taaggaaggt caaacgatcc ttgatttcct tgagaatggg acgagcaatg atggcttcat
     2581 tttcagacaa agttaactgg ctcaccaagt ccaagtgatc tgcgatagac aggtctgaaa
     2641 tttctccgat atgtggccct tgctcaccac ccacacggac agacaaggcc tggtcattga
     2701 gacgataacc gtgacaggtt ccgcaagtca gctcattcat gtagaggcgc atctgagtgc
     2761 gagtgtaatc gctattggtt tcatggtaac gacgtttgat attattgata actccctcaa
     2821 acggaatgtc gatatcgcgc acgccaccaa attcattctc atagtggaaa tggaatttct
     2881 taccatctga gccgtagaga atcaagttct tgtcttcttc tgacaagtcc tcaaaaggct
     2941 tatccatagc cactccaaaa gccgtcatgg cctgctctag catgtttgga tagtagttgg
     3001 atgagatagg attccaaggt gccagcgctc cctcatgtaa ggttttgctg gcatttggca
     3061 ctaccaaatc agtatccacc tccagcttga tgcccaagcc gtcacactca ctacaagagc
     3121 caaaaggagc attgaaagaa aagagacgag gctctaactc tgggacagta aaaccacaaa
     3181 ctggacaggc ataatgctca gagaacaaca actcagagtt gtccatggtg tcgataatga
     3241 cataaccttc tgcaatacga agggcagcct caatggaatc aaagagacga ctacgaatgc
     3301 cctccttgat aacaatacgg tcaaccacga catcgatatt gtgttgcttg ctcttagaca
     3361 actctggcac ttcggtcaca tcatagactt ccccatccac acggacacga acatacccgt
     3421 ctttctgaac cttctcgata acactcttat gttggccttt tttcttgcgg atgacaggag
     3481 acaagatctg caagcgctgg cgttcaggta actccaaaac cttatcaacg atttgctcca
     3541 cagaagaagc cttgatagcc ccatgcccgt tgatacagta aggcgtcccc acacgcgcgt
     3601 agaggagacg cagatagtca ttgatttcag tcgttgttcc caccgtcgag cgagggtttt
     3661 tgctagtcgt tttctggtcg atagaaatag ctgggctgag accatcaatg gcatctacat
     3721 ctggtttttc catatttccc aagaactgac gagcgtaggc ggacaaactc tctacatagc
     3781 gacgttgtcc ctccgcatag agggtatcaa aagccaaact ggacttccct gaacctgaca
     3841 agccagtcac gacaaccaac ttgtctcgcg gaatctccac atcaatattt tttaaattat
     3901 gggcacgcgc cccatgaatg acaattttat cttgcatctt tgttctttct agtccattat
     3961 tgcttaccat tataccaaaa aaagtgagat tctattaccc aaaaggccga ttttgtagta
     4021 taatagtaca gtgtgaaaaa atctgaaaaa tgagaaagga taagggatat gaaacaagtt
     4081 tttctctcta caacaactga atttaaagag atcgatacgc ttgaaccggg tacttggatc
     4141 aatctcgtca atccgactca aaatgaatca ctcgaaatcg ccaacacctt cgatattgat
     4201 attgccgacc ttcgagcacc gctcgatgcg gaagaaatgt ctcgtattac cattgaagac
     4261 gagtataccc tgattatcgt agacgtgccg gtcacggagg aaagaaataa ccgcacctac
     4321 tacgtaacca tcccgcttgg tattatcatc actgaggaaa ccattatcac tacgtgtttg
     4381 gaaccactac ctgtccttga tgtctttatc aaccgtcgat tgcgtaattt ctataccttc
     4441 atgcgttcac gttttatctt tcaaattctt tatcgcaatg cagagcttta cctaacagcc
     4501 cttcgttcaa tcgaccgcaa gagtgaacaa atcgaaagtc aactgcatca atcaactcgt
     4561 aatgaagaat tgattgagct catggaattg gaaaaaacta tcgtctattt caaggcctcc
     4621 ctcaaaacaa atgagcgcgt gattaagaaa ttgaccagtt caaccagcaa tatcaagaaa
     4681 taccttgagg acgaagacct gcttgaagac accctgattg aaacccaaca ggccatcgag
     4741 atggcagata tttatggaaa cgtcttgcat tctatgacag agacctttgc ctctatcatt
     4801 tctaacaacc agaacaacat catgaaaacc ttggcccttg tgaccatcgt catgtccatc
     4861 ccaaccatgg tcttttctgc ctacgggatg aactttaagg ataatgaaat tcccctaaac
     4921 ggagagccaa atgccttctg gttaatcgtc tttatcgcct ttgctatgag tgtctcgctc
     4981 actctctatc tcatccataa aaaatggttc taaaaggagt tcctatgtct caaattgatc
     5041 tacaaaaatt aactaagaaa aaccaagagt ttgtccacat tgctacccaa caattcatca
     5101 aagatgggaa aacagacgct gaaatccaga ctatttttga ggaagtcatt ccccaaatcc
     5161 ttgaggagca atctaaaggt acaactgccc gttccctata cggcgcacca actcattggg
     5221 ctcatagctt cactgtcaaa gagcagtacg aaaaagagca tccaaaagaa aatgatgacc
     5281 caaaactgat gattatggac tcagctcttt tcatcactag cctctttgcc cttgtcagcg
     5341 ccctcacaac cttctttgcg gcagatcaag ctttcggcta tggattgatt actcttctat
     5401 tagttggact ggttggtgga tttgccttct acttgatgta ctactttgtt taccaatact
     5461 atggaccaga tatggatcgc agtcaacgtc cacctttctg gaaatctgta ctagttatcc
     5521 tagcttctgt gttcctttgg ttgcttgtct tctttgcaac aagcttccta ccagctagcc
     5581 ttaacccagt actggatcca ttgccactag ctattattgg agcagccctc ctagcccttc
     5641 gcttctatct caagaaacgc ttgaatatcc gtagtgcaag tgcaggacca acacgctatc
     5701 aagaataaga aaacgataaa agcaactgca ggtgcggttg ctttttcact tacttttttg
     5761 a
//
LOCUS       DAVBHS010000126         5757 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVBHS010000126 DAVBHS010000000
VERSION     DAVBHS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.126"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..281)
                     /locus_tag="VQ651_001690"
     CDS             complement(<1..281)
                     /locus_tag="VQ651_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2754653.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIA"
     gene            complement(323..1831)
                     /gene="glpK"
                     /locus_tag="VQ651_001691"
     CDS             complement(323..1831)
                     /gene="glpK"
                     /locus_tag="VQ651_001691"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET2754654.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            complement(1990..3417)
                     /locus_tag="VQ651_001692"
     CDS             complement(1990..3417)
                     /locus_tag="VQ651_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2754655.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLDSAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            3582..4454
                     /gene="hslO"
                     /locus_tag="VQ651_001693"
     CDS             3582..4454
                     /gene="hslO"
                     /locus_tag="VQ651_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET2754656.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQERHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGAGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            4441..5421
                     /gene="dusB"
                     /locus_tag="VQ651_001694"
     CDS             4441..5421
                     /gene="dusB"
                     /locus_tag="VQ651_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET2754657.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
BASE COUNT         1648 a         1370 c         1126 g         1613 t
ORIGIN      
        1 gcgatttgtt gaaccactgc acgttcagaa accgtatctg agaccacttc tacgtcgaat
       61 tgtttaaggt aacgaagtcc tccgtgaacc aattttgttg aacgactaga tgttccttct
      121 gcaaagtctt gcatttcaat caaaccagtc tcaagaccgc tagctgccgc ctgcaaggct
      181 acaccagctc ctgtgattcc tccaccgata atcaagaggt ccagggtgcg ttcctgcatt
      241 tttttaattg acaattcacg tgttttcttt gaaaattcca tatttacaca ctttctaaat
      301 aaatcgcttt attctgccag tattagtcgt ctacttccgc aaagacttga gttgctttca
      361 cagccttctt ccagcccttg tagagttgtt ccttgcgaga ttcgttcata gatggctcaa
      421 agagttctcc tgtctcgttc aagagtttca actcgtccaa gtctttccag taccctactg
      481 acaaacctgc taggaaggcc gctcctagag ctgttgtttc caggttttta gcacgtgcaa
      541 tgtcaatgcc taaaatatcc gcctggaact gcatgaggaa gttgttcatg gctgcaccac
      601 catccacctt cagtacttga atggcggtct gagtatccac ttgcatggtg tcgatgatat
      661 cacgcacttg ataagcaata gattgcaaag tcgccttgat aaagtcttct ttgcttgttc
      721 cacgagtcaa accaaagacg gaaccacgag cattttggtt ccagtatgga gcgcctagac
      781 ctgtaaaggc tggaacgaca taaacttcat cgttgttgtg agaatcacga gcgtattttt
      841 cagattctgg tgaattttca accatgcgaa gaccgtcacg aagccactga atagcacttc
      901 ctgcgatgaa gatagaacct tccaaggcat aataaacctt accgttgatt ccgtaaccaa
      961 tggttgtcaa gaggttgttt tcagacaact gcatctcttc cccagtattc atgatgatga
     1021 aagagcctgt tccataagta ttcttaacca tacctggctc aaaagccaac tgtccaaaga
     1081 gggctgcttg ttggtcccca gccatacctg agattggcac ctctccaccg tagaaatgga
     1141 atggagctgt cttgccgtag atttcggagt tagaacgaac ttctggaagt atagccttcg
     1201 gaatgttaag gatttccaaa atctcatcat cccatttgag ttctttaatg ttataaagca
     1261 tggtacgagc tgcatttgag tagtcagtca cgtgagccgc accgtcagtc aatttccaaa
     1321 ccaaccaagt atcgatagta ccaaagagca attccccttt ttctgctcgc tcttgagcac
     1381 cttctacatg atccaaaatc caacgaacct tggtagcaga gaagtaagca tcaataatca
     1441 aaccagtctt ttcatggaat ttttccacat aaccttggct ttttagttgc tcagccaaag
     1501 gtgctgtctg gcgtgactgc caaacgatag cattgtagat aggaagtcct gttttcttat
     1561 cccagacaac cgttgtttca cgttggttgg taatcccgat tgcctcgatt tgatttggct
     1621 tgacaccact ttcgatgaaa gcacccgcaa taactgactg aacagagttc caaatttcat
     1681 tggcattgtg ctcaacccaa cctgcctgag ggaaaatctg ggtaaactct ttttgactcg
     1741 agctaacctt ttcccctttt ttgttgaaaa tgatggcacg agaacttgta gttccctggt
     1801 caatggccat gatgtatttt tcttgtgaca tgtactgtcc tcctgataaa gatcttgagg
     1861 tgtttcctct ttacacttac tagtatacaa aataaagcgc tttcttgttt atcaactttt
     1921 tttaattttt aaaaaaatgt gaggagattg tgtgaatttc acaaaaaaga cctagattaa
     1981 ccaagccttt taagtgaata ataactgacg aatccctgcc aaatcttcca tatccaagtc
     2041 gttttttaaa taatagactg gtgtctgttc cttcttatga atcgggttat tggtaaccag
     2101 caaatcataa tgccgactag ggtcataagc ttcaatggta ataaaacgat tgtattccaa
     2161 ataccgtttc aaaatggctg ccatccttga aaagacaaga aaaccagatg tcaaatccag
     2221 accaatccgc atatgctggc caccgttttc agccatatat tcgattaact ggagccattc
     2281 ccagagaatc ttcttatcta aatctgtccc ctgttgaaaa gcaggtagag catgaaaaat
     2341 ctcctctgct gtctctttaa aatcatgttc catcagtaaa tatggatgac gattctctaa
     2401 ctggtacttg tagcgatcct gtaaaatata gcccttatag agatagactt gagcacaaag
     2461 ctgactgagt tcataggtga catggtcctc tgtataatcc cccaatagtt cctccttctt
     2521 catttcttga atcaattgcg tcagtaaatc tgccaactgc cctccaaaac caagaatata
     2581 ctccatagta tgaagaggaa gaatgcgatg agataggaga aaagagaaga atatcatcat
     2641 ctcaccatcc tcaactccta gtggaatatg ttgcccagca aaaaaggacg gaacctttct
     2701 cagcaaacga agataaaaga tattgtcaaa ataccctcgc attttctctt ctatgacttg
     2761 aaactgacaa gcattgaccc gaagacgttg ttgactgatg tgagcccaga gaaccaagtc
     2821 caatttctgc cccgcagaca aacttgcacc gcagatttcc tctaaattgg caatctcctg
     2881 ttttctctct ggtttctgca tgtgaccttc ccattcctga ctcgaccaga cctttcggaa
     2941 aagacagaaa tagaaatagc gaatctgatg ctctggacct ctccagcgtc cattttggat
     3001 ggataaatca aattctgaca aaatctgatt taaaccagcc aagtgacgac caagcgtagc
     3061 ctcgctaatc accaattctt gagccagctg atgggctaaa aactgttggt ggtagagaag
     3121 ataaaccaaa atctggtatt taacagcact atccaaaaac aagctccgaa tatctctccc
     3181 cttggtagct gcaccgatag acagacgcag attttcatct tctgagtgaa tagtcagttc
     3241 taagccacta tccaaagcct tgtcattgag cagggtgaca tatttggtta gggttgcttt
     3301 tgaaaatcct gtttcttcca ttacagcctt gacggtcgtc tgagactctt gtaatagaaa
     3361 ggaaaggatt gaaaattgac cacactcggc tttctccatc aaatctccta aatacatttg
     3421 ttaccccttt cattttctac cttaagcata gcataaatct tacaaatgct gaaataatct
     3481 gtttctcttt ttattttcat gctgatttcc tggtccatta tcctgaaaat cagtaaacac
     3541 acggctcccc ctttgggcat ttttatgcta aaatagtagc tatggataaa attattaaaa
     3601 ctatatcaga aagcggagcc tttcgtgctt ttgtccttga tagcacggaa accgtccgca
     3661 ctgctcaaga aagacatcaa acccaagcta gctcaactgt agcgcttggt cgaactctta
     3721 tcgctagcca gattctcgca gccaatgaaa aaggaaatac caaacttaca gttaaggtgt
     3781 tgggctctag ctctctaggt gctattatca ccgtcgctga taccaagggg aacgtcaaag
     3841 gctatgttca aaatcctggt gttgacatca aaaagactgc gactggtgaa gtcctagtcg
     3901 gaccttttgt tggaaatggt caattcctcg ttatcacaga ctacggtgct ggaaatcctt
     3961 acaactctat aactcccctc atctctggag aaatcggtga agaccttgcc ttttacctta
     4021 ctgaaagcca acaaacgcct tcagcggtcg gcctcaatgt ccttttggac gaggaagaca
     4081 aggtcaaggt tgcaggtggt ttcctagttc aagtcttgcc aggagccaag aaagaagaga
     4141 ttgctcgctt tgaaaaacgc atccaagaaa tgccagctat ctctactctt ctcgaaagcg
     4201 acgaccatat cgaagccctc ctcaaggcta tctacgggga cgaagcctac aagcgtcttt
     4261 ctgaagaaga aatccgtttc caatgtgact gtagccatga acgctttatg aacgctcttg
     4321 ccagccttcc aagctcagac ttacaggaaa tgaaagagga agaccacggg gcagaaatca
     4381 cttgtcaatt ctgccaaact acttacaact ttgatgaaaa ggacctggag gaactcattc
     4441 gtgacaaatc ttaatacacc ttttatgatt ggcaatattg agattcccaa tcgtaccgtt
     4501 ttagcgccta tggctggcgt gaccaactca gcctttcgta ccatcgcaaa agagctcgga
     4561 gctggactcg ttgtaatgga aatggtctct gacaagggaa tccaatacaa caacgaaaaa
     4621 accctgcaca tgcttcatat cgatgagggc gaaaaccctg tctctatcca actttttggt
     4681 agcgatgaag acagcctagc acgcgcagca gaattcatcc aagaaaacac caagaccgat
     4741 atcgtcgata tcaacatggg ctgccctgtc aacaaaatcg tgaagaacga agctggagct
     4801 atgtggctca aggatcctga caagatctac tctatcatca acaaggtcca gtctgtcctt
     4861 gatatcccac ttactgtcaa aatgcgtacc ggatgggcgg acccatctct tgcagtagaa
     4921 aatgccctcg ctgctgaggc tgcaggtgtt tctgccctcg ccatgcatgg ccgtacccgt
     4981 gaacaaatgt atactggcca cgcagacctt gagacccttt acaaggttgc ccaagctcta
     5041 accaagattc cattcatcgc caacggtgat atccgtactg tccaagaagc caagcaacgc
     5101 atcgaagaag ttggtgctga cgcagtcatg attggccgag ctgccatggg aaatccttac
     5161 ctcttcaacc aaatcaacca ttactttgaa acaggagaaa tcctacctga tttgaccttt
     5221 gaagacaaga tgaagatcgc ctacgaacac ttgaaacgat tgattaacct caaaggagaa
     5281 aacgtcgcag ttcgtgaatt ccgcggcctc gctcctcact atctccgtgg aacatctggc
     5341 gctgccaaac tccgtggagc catttcgcaa gctagcaccc tggcagagat tgaagccctc
     5401 ttgcaattgg agaaggctta atagtttaaa acccgtaact ctcttaaaga gcctcttgaa
     5461 tgccgccatt ctttcctcat caaaaaaact ccgtagtctc tagcgctata aatccggcta
     5521 gagattacag agttattttt ttatctaaac ttcctacaag ggtatattat ctactgaaag
     5581 gatatggtat ttgtagggaa tactttaaag agttcttaca ggaagtcagt attaactagt
     5641 tatattaggt ttagtttacc cattcaccat tggcattgac tctatagcta tctacagttg
     5701 tgttgactgc aagggcacct gagccattga catagtacca tttatctgat actttga
//
LOCUS       DAVBHS010000127         5739 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVBHS010000127 DAVBHS010000000
VERSION     DAVBHS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.127"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            155..1174
                     /gene="galE"
                     /locus_tag="VQ651_001695"
     CDS             155..1174
                     /gene="galE"
                     /locus_tag="VQ651_001695"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2754658.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAIEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            1176..2129
                     /locus_tag="VQ651_001696"
     CDS             1176..2129
                     /locus_tag="VQ651_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2754659.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRKLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDDRLKKIEFI"
     gene            2281..2946
                     /locus_tag="VQ651_001697"
     CDS             2281..2946
                     /locus_tag="VQ651_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754660.1"
                     /translation="MLSKEDYTEEIGLIKKQNYVEAELYPIVADIIKPTLKDSLSKRY
                     VFGRQRRGLGQIYYGLSNFPDIVILDKTYENKSRKSIKIEEWKKLRGCVEVKNLNYSL
                     ITEEKIKSTISNSFEHITGEMEQLTGEMGQLIGDLLWYKKVIYTNGIEWRFLSLDDKE
                     EIDNTIVEVVNKRIETEEAGNSFDWWKNIKDLSFNYTDICLSKDCRQEWNEFVKRVKE
                     IEW"
     gene            2985..3086
                     /locus_tag="VQ651_001698"
                     /pseudo
     CDS             2985..3086
                     /locus_tag="VQ651_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group 2 glycosyl transferase"
     gene            complement(3137..3343)
                     /locus_tag="VQ651_001699"
     CDS             complement(3137..3343)
                     /locus_tag="VQ651_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET2754661.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            3395..3871
                     /locus_tag="VQ651_001700"
     CDS             3395..3871
                     /locus_tag="VQ651_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET2754662.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            3880..4551
                     /locus_tag="VQ651_001701"
     CDS             3880..4551
                     /locus_tag="VQ651_001701"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET2754663.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDFETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            4682..5020
                     /locus_tag="VQ651_001702"
     CDS             4682..5020
                     /locus_tag="VQ651_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HET2754664.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            5023..5652
                     /locus_tag="VQ651_001703"
     CDS             5023..5652
                     /locus_tag="VQ651_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HET2754665.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
BASE COUNT         1794 a          975 c         1265 g         1705 t
ORIGIN      
        1 ccatggcaag gcgacgttga cgtggtttga attggatttt cgaagagtat aagcatttta
       61 ctgaaatttt atcaggtagt ttaggatggc aaatgacatt ccctgtcaaa aatggtaaaa
      121 taagaaaaaa taatctgaga atcgaggaaa aaagatgcaa gaaaagattt tggtaactgg
      181 tggtgccggt tttattggaa cccacactgt tattgaattg atccaagcag gccatcaagt
      241 tgttgtggtg gataatcttg tcaatagcaa tcgtaagagt ttagaagttg ttgaaagaat
      301 cacaggagtt gagattcctt tctatgaggc agatattcgt gacacagcta ccctcagaga
      361 tattttcaag caggaagaac cgactggtgt cattcacttt gctggtttga aggctgttgg
      421 cgaatcaaca cgtatccctc ttgcctacta tgacaacaat atcgctggaa ctgtcagcct
      481 tttgaaagcc atagaagaaa acaactgtaa aaacatcatc ttcagttctt ctgcgacagt
      541 ttacggggat ccgcacacag tgcccatctt ggaagatttc ccactttcag tgaccaaccc
      601 atacggtcgt actaagctca tgctagagga aattttgact gatatttaca aagcagactc
      661 agaatggaat gttgtcttgc ttcgttactt taacccaatc ggagcccatg agagtggtga
      721 tttgggagaa aatccaaacg gtattccaaa caatctcttg ccatatgtga ctcaagtagc
      781 cgttggaaaa ttagagcaag tgcaagtgtt tggagacgat tacgatacgg aagatggaac
      841 aggtgttcgt gactatatcc acgttgtcga tttggctaag ggtcacgttg cagctttgaa
      901 aaaaatccaa aaaggttcag gactaaacgt ttataacctt ggaactggta aaggttactc
      961 agttcttgaa attatccaaa acatggaaaa agcggtggga cgtcctattc cttaccgtat
     1021 cgtagaacgt cgcccaggtg atatcgctgc ctgctactca gacccagcaa aagctaaagc
     1081 agaactcggt tgggaagcag aactcgacat cacccaaatg tgtgaagacg catggcgttg
     1141 gcaaagcaag catccaaatg gatttgaaga ctaagatgat gatttcaatc atcgtccctt
     1201 gtttaaacga agaggaagta cttcctcttt tttatcaggc tctggaagct ttacttccag
     1261 atttggaaac agaaatcgag tatgtctttg tcgatgatgg atcaagtgat gggaccttgg
     1321 aactcttaaa ggcctatcgg gagcaaaatc cggcagtcca ttatatttct ttctctcgaa
     1381 attttggcaa agaagcagcc ctttatgcag gcttgcaata tgcgacagga gatttggtgg
     1441 tggtgatgga tgcagacctc caagatcctc ctagtatgtt gtttgagatg aaaaatgtac
     1501 tagacaaaaa tgtagacttg gactgcgttg ggacacggag aactagtcgg gagggagaac
     1561 ccttctttcg cagtttctgt gctgttctct tttatcgcct catgcaaaaa atcagcccag
     1621 tggctctgcc gtcgggtgtc cgtgattttc gtatgatgag aaggtctgta gtcgatgcca
     1681 ttttaagctt gaccgagtcc aatcgttttt caaaggggct cttcgcctgg gtgggtttca
     1741 aaacctacta tttggactat ccaaatgtcg aaaggcaggc tggcaagacc agttggagtt
     1801 ttaggaaact ctttttctac tccattgaag ggattattaa tttttcagat tttcccttga
     1861 ttatagcctt tgtagctggt ctcctatctt gttttctttc tctgctgatg accttttttg
     1921 ttgtggttcg gaccctcatt ttgggcaatc cgacatctgg ttggacctct ctgatggctg
     1981 ttattctctt tcttggaggc attcaactct tgaccattgg gattctcggc aagtatatca
     2041 gtaagattta tttagagact aaaaaaagac cactttatct tatcaaagaa aaaagtgatg
     2101 ataggctaaa aaagatagaa tttatctgac atagtcactt tttgttattt gtaattcttt
     2161 tagttcggag tttgggtgat ttttaaaaga attagggatt tgaaatgatt taaaaaatct
     2221 atgtagacta aatttaaatt gtagtgactt aataaataaa aaatttagga gtattatgtt
     2281 atgctaagta aagaggatta tactgaagaa atcgggctaa taaagaaaca aaattatgta
     2341 gaggctgaat tgtatcctat agttgctgat ataattaagc caactttaaa agatagttta
     2401 tcaaaaagat atgtcttcgg aagacaaaga aggggtttgg gacaaattta ttatggtcta
     2461 agtaattttc ctgatatagt tattttagat aagacttatg aaaacaaatc taggaaaagt
     2521 ataaaaattg aagaatggaa gaaacttcga ggatgcgtgg aagttaagaa tttaaattat
     2581 agtttaatta cagaggaaaa aatcaaatca accatatcga acagttttga acatataact
     2641 ggagagatgg aacaactaac tggagagatg ggacaactta ttggagacct tttatggtat
     2701 aaaaaagtaa tttatacaaa tggaattgaa tggagatttc taagtttaga tgacaaggaa
     2761 gagatagata atacaattgt tgaagtagtt aacaaaagaa tagaaacaga agaagcggga
     2821 aattcttttg attggtggaa gaatattaaa gatttatcgt tcaattatac ggatatatgc
     2881 ctatctaaag attgtagaca agagtggaat gaatttgtaa aaagggtaaa ggaaatagaa
     2941 tggtagaagt ctattctcat tgagaaattt agattttaag ttacagtaag atttatttag
     3001 agactaaaaa aagaccactt tatcttatca aagaaaaaag tgaccttcct gattttacag
     3061 aaaaaaataa agtgaaaaga ctataatttt acatggaaat gtgctaaact agagggagtg
     3121 gattatcgcc attgatttag gcttcctggt ttccaatcag ggcgcgagtt gggcaatttt
     3181 taacagcctc taagacatcc tgactaggag aaatttcttt ttccagttgg tcagggtcat
     3241 cgtaaaaacg cacgattcca ttatcgtggt aatcaaataa atcagaataa gtttggcaaa
     3301 gcccacaggc gatacatcgt tcaggtataa gtgtgatttt catgttcata tttatattgt
     3361 aataagaaaa gtaaaaaaaa acaaggagta aggtatggca aaagaaccgt ggcaagaaga
     3421 tatctatgat caagaagaat caagagcaga gcgtcggcat cgaaaccacg gaggggctga
     3481 taggatggct aatcgtattt tgacgatcct agctagtatt ttctttgtaa ttgtggtggt
     3541 gatggtcatc gttctcatct atctatcatc ggggggaagt aatcgcacag cagccttaaa
     3601 agactttcat gattctgatg caagtgtagt acaaatctca tcttcaagca gttctcagcc
     3661 tgagcagagt tcagagccag aatctacttc tagtagttca gaagaagctg ctaatcctga
     3721 aggaacgatt aaagttctcg caggagaagg ggaagcagct attgccgctc gtgcaggaat
     3781 ctccattgct cagttagagg ccttgaatcc tgggcacatg gctacaggat cttggtttgc
     3841 taatccaggt gatgttataa aaataaaata ggagccgata tgaaaacaat tcaaattgct
     3901 attgatggtc ctgcttccag cggtaagagt acggtcgcaa agattattgc taaggatttt
     3961 ggattcacct accttgatac aggagctatg tatcgtgcag cgacctatat ggctcttaag
     4021 aaccaattag gagttgaaga agtcgaagcc cttctagcct tgttggacca gcatccaatc
     4081 agctttggac gttcagaaac tggagaccag cttgtttttg taggagatgt ggatattacc
     4141 catcctatcc gtgaaaatga agtgaccaat catgtttctg ctattgcagc aattcctgaa
     4201 gtgcgtgaga aactggtttc tctccaacaa gaaattgccc agcaaggcgg gattgtcatg
     4261 gatggtcgcg atattggaac tgttgtattg ccacaagcag aattgaaaat tttcctagta
     4321 gcttctgttg atgagagagc agagcgtcgt tacaaggaaa atattgccaa gggaattgaa
     4381 acagactttg aaaccctaaa aaaggaaatt gctgcgcgtg actacaagga tagtcatcgt
     4441 gagacttctc ctctcaaaca agcagaggat gctgtctacc ttgatacaac tggtttgaac
     4501 attcaagaag tagttgaaaa aatcaaagca gaagctgaaa aaagaatgta aatgtagaaa
     4561 agccgataga ttgatatcgg tttttttcta aatttgtcaa aatgctgatt ttagggtata
     4621 atagttgctg ttcgagaaat tttgattttc aaagaatagt gaatgatgat aaggagaaac
     4681 catgaacaat ttaccaaatt gtccaaaatg taactcagag tatgtctacg aagacggtgc
     4741 cctactggtt tgcccagagt gtgctcatga gtggaatcct gctgaagttg cagaagtaga
     4801 agaaggtctt gtcgctatcg atgccaacgg aaataaattg gctgatggtg atacagtaac
     4861 tctcatcaag gacttgaaag taaaaggtgc gccaaaagat ttgaaacaag ggacgcgcgt
     4921 gaaaaatatc cgcatcgtag aaggcgacca caatatcgac tgtaagattg atggttttgg
     4981 tgccatgaaa cttaaatcag agtttgtgag gaagatttaa gcatgtcaaa acactataaa
     5041 cttgtatttt atagccgtat cttcttgttt ctagcggctt ttacgggagt ttatcttgaa
     5101 attactaagc atggtggttt tgggatgctt ctctattaca cggttctgtc caacctcttg
     5161 gtaactattt ttacccttta tcttctaaag gttatgagcc gtgtaggtga aaattggcaa
     5221 aggccaagtc tcttgcgctt aaaaggtggg gtcaccatga gtatcatgat tacctgtgtg
     5281 atttaccatt tcctcttggc gcccattgcg actaatttct ataccctaga aaatttcctt
     5341 tgccactata tcgttcccat ctggttttta gcggataccc tcttttttga caaacagggt
     5401 caatacaaga tttgggatcc agcagtgtgg accattttac cctttctgta tatgatgttt
     5461 gctcttttta atggcttggt tctaaaactc aatattccaa atgccaagga taatcctttc
     5521 ccttacttct ttttgaatgt gaacaagggt tggaatgttg tgtttaagtg gtgtctgatt
     5581 atctttgttg cctatatggt agcaggattt attttctact ttatcaagca aatcaagaga
     5641 aagtcatcct aatactcttc gaaaatctct tcaaaccacg tcagtttcca tctgcaacct
     5701 caaaacactg ttttgaacaa cctgcggcta gcttcctag
//
LOCUS       DAVBHS010000128         5659 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVBHS010000128 DAVBHS010000000
VERSION     DAVBHS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.128"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(97..786)
                     /gene="rplA"
                     /locus_tag="VQ651_001704"
     CDS             complement(97..786)
                     /gene="rplA"
                     /locus_tag="VQ651_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET2754666.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMGLVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(993..1418)
                     /gene="rplK"
                     /locus_tag="VQ651_001705"
     CDS             complement(993..1418)
                     /gene="rplK"
                     /locus_tag="VQ651_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET2754667.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAVRMIEGTARSMGFTVID"
     gene            complement(1541..2257)
                     /locus_tag="VQ651_001706"
     CDS             complement(1541..2257)
                     /locus_tag="VQ651_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET2754668.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKAKNEDGAAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     VIGTNGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYFGFEGGDYVD"
     gene            complement(2260..2730)
                     /locus_tag="VQ651_001707"
     CDS             complement(2260..2730)
                     /locus_tag="VQ651_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET2754669.1"
                     /translation="MCLICQRIELIKKGENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVSELHHLEKKTRIRFLEEMSVVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     RGDMNGYGLNGRGPVWWIPFEEMTAEICQSKPDEIKRLVERLSSEVDKLLEIKE"
     gene            complement(2732..3769)
                     /locus_tag="VQ651_001708"
     CDS             complement(2732..3769)
                     /locus_tag="VQ651_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET2754670.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVLGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAMLLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTNEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSALVD"
     gene            complement(3867..5192)
                     /gene="brnQ"
                     /locus_tag="VQ651_001709"
     CDS             complement(3867..5192)
                     /gene="brnQ"
                     /locus_tag="VQ651_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HET2754671.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(5363..5455)
                     /locus_tag="VQ651_001710"
     CDS             complement(5363..5455)
                     /locus_tag="VQ651_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2754672.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(5498..5650)
                     /locus_tag="VQ651_001711"
     CDS             complement(5498..5650)
                     /locus_tag="VQ651_001711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2754673.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
BASE COUNT         1629 a         1262 c         1076 g         1692 t
ORIGIN      
        1 gaggacttac acctcagata tacgacatgc ccgtcgtaca tcaaaaaaag ttgagacata
       61 gcgtcttcaa cttttttaca ttactttaac tactgattaa agtgagttta cgtcaacttt
      121 gataccaaca ccttgagtag ttgtgatagt caagtttgtt acgtaagttc ctttagctgt
      181 agctggtttt gctttttgga ttgtttcgtt gaaagcttta aagttttcaa ccaatttttc
      241 agcttcaaat gatactttac cgatgattgc ttgaacgtta cctgcacggt cagcacggta
      301 agtgatttta ccacctttag actcttcaac tgctttagca acatccattg ttacagtacc
      361 agttttaggg tttggcatca agttacgtgg tccaaggaca cgtccaagac gtccaacaag
      421 acccatcata tcaggtgtag cgataactac gtcgaagtcc aaccaaccgt cgttgatttt
      481 agcaacaagg tcatcttcac caacaaagtc tgcaccagca gcttttgctt cttcagcttt
      541 tgcaccacgt gcgaaaacaa gaacgcgtga agttttacca gtaccgtttg gcaataccat
      601 tgctccacgg atttgttgat cagctttttt aacgtcgatg ttcaagttgt aagcaacttc
      661 tacagttgcg tcaaattttg caaagtttgt ttcttttgca agtgctacag cttcttctac
      721 gctgtatgct tttgtgctgt cgattttctc aagagcagca cgaagttgtt tgcttttttt
      781 agccattttc tattctcctt gtaagtggtt caatcgattt tcatctccca cgtcacttct
      841 cgaaatgatg aagtcttgcg ggttttcagt ctattagatt cttttcctac ggccttaagc
      901 tcccttgctg tacccaagta caagcatcag tcacttgcct agtatgaaaa gcaactaata
      961 gactgctgtg agattttcta ctgtgttatt gattagtcaa taacagtgaa tcccatagaa
     1021 cgagcagtac cttcgatcat acgcactgca gactctacgt ttgctgcgtt caaatctggc
     1081 atcttagttt ctgcaatttc ttgtacttgt gcacgagtaa ctgtagcaac tttagtttta
     1141 ttaggtgtac ctgatccttt ttcaacacct gcagcttttt tcaaaagaac agcagctggt
     1201 ggtgttttcg taacgaaagt aaatgattta tcttcgtaaa ctgagataac aactggaatg
     1261 atcataccag cttggtcagc tgtacgagcg ttgaactctt ttgtgaatcc catgatgttg
     1321 ataccagctt gaccaagagc aggtccaacc ggtggagctg gtgtagcttt accagcaggg
     1381 atttgcaatt ttacaagttt ttcgactttt ttagccattt ttaaatcctc ctttgtggtt
     1441 ttggcggtaa ttagagattt ttacctccca caagtatgtt tttcacatac ccatctatta
     1501 tacactattt atctggaatt gcaagagaaa agtttatttt ttaatcgacg taatctccac
     1561 cttcaaagcc gaaatactct tccaaactga ggccactagc tgcaatctct ttagcctctt
     1621 ttccgacata acgaaggtgc cattcctcag ccatatagcc tgtttccttt tccttacctt
     1681 tgagataacg tacaacaaag ccataatcag ccgcatgatc caagagccat tgggccgctt
     1741 tttcttctgt caccaaatca ccatttgtcc caatcacatc aaaggctaaa cctgtctggt
     1801 gctcgctata gccaggacgg gcagaataac ggtcggcagc ttctttccca tcttgattga
     1861 cataatcttg atagagcttg gtctgagttt cataacttct aaagccactg taatgatcgc
     1921 taatggggaa acctgcctct tgcatagctt tgatgagctt gaccaactca gccttggctg
     1981 ttggattttc ccctggattg tagtcttttg acaatggata gtgtttgttg gctacgatga
     2041 tttcatcgta tttcccttga atgctgtaat agtctccttt gttaaccact tctgcttttt
     2101 tctgggcagc tccttgagac ttacttccta ctgttccatc agctttagca gtttgctctg
     2161 tcttagctgc tccatcttca ttttttgctt tttcttgtga acaagctgct agactcaagg
     2221 ctagcaaggc tgtcaagaca aggtatcttt ttttcatctt tactccttta tttctaatag
     2281 tttatctact tctgatgata aacgttcgac taatctttta atctcatcag gttttgattg
     2341 gcagatttct gctgtcattt cttcaaaggg aatccaccat actggaccac gtccattgag
     2401 gccataacca ttcatatcac ctcttcgtct aggaaacaga tgccaatgga gatgagcatc
     2461 gccatttcct agtagttcga tattcatttt ctcagcagca aaggccttgg caactgcctc
     2521 ttggaccaca ctcatttctt ctagaaaacg tattctagtt ttcttttcta aatgatgcaa
     2581 ttcgctgaca tgttccttgg ctagaaagag actatagcct gcaaaatact ggtggtctcc
     2641 aatcacaaga tagcctgttt ccaactcttt gacaaaatag ggattttctc ccttcttgat
     2701 gagctcaatt ctctggcaaa tcaaacacat attagtctac caatgcactc ttaagatagg
     2761 catcgaccat tcgttctgtt gcgaccaccg agtcaatatg agtgcgctca taagaatgac
     2821 tagactcgat acccgcacca agaagggcat gcttgacctc tgcccctgca gacatagccg
     2881 ctgaagcatc cgaaccataa aatggataga tgtccagctt aaaaggaata tcttgctctt
     2941 tagccaaggc aaccaagtgt tgacggaagt catagtgata aggacctgag gcatccttga
     3001 cacagataga tactgtgtac tcatttgtct gctggtcatc tcccatagct cccatatcca
     3061 cagccagata ttctactacc tgagcaggaa tgttagaatt agcaccatgt cctacttctt
     3121 caaaaactga aaaagcaaaa tgagttgtta ctggcaattc aatcttctct ttcttataaa
     3181 tacgaagcag gttgagcaac atcgctgcgc tgaccttatc atccaaatga cgagacttga
     3241 taaaacctgt atcagtcaca acagttcgtg gatcgaagct gataaaatca ccgacctcaa
     3301 tcccaagagc acgagtttct ttttcattgc ttacttttac atccaaacgc acttccatat
     3361 tgtcctgcgt gcgttctgca gttcctgcat ccttgtagac atggcaagaa gtttggtgga
     3421 tgaggatggt tcctaatacc ttttgacctg tgctagccac atgaacggta cagttttcac
     3481 cttcaatcat gttccaagga aaaccaccga tacggtccaa tttaagacga ccatctggtt
     3541 tgacagcacg aacaatagca cccagtgtgt ccacatgggc agtcacatag cgatgttgct
     3601 catcattttg acctttgatg gtcacattga caccaccctt ggctgtgcga actggctggt
     3661 aaccaaaacc ttctagagtc ttgactaaat aattcgaaat ttcacgagtg aagcccgttg
     3721 gcgatgcgat ggctgtcagt tctttgatat attctactgt ttgattcatt tcttcacctc
     3781 tttcgctacc tattataaca catttatcat cggataaaaa aagaaaatca ctcctgtaga
     3841 cttggctaca aaagtgattt aagtgattat tccatttcaa aaacatcgct ttcttgcttg
     3901 tttggtagaa ccaatgagag caagattcca acaatggctg gcaccaacca tgggagagat
     3961 gccttggcaa aaggaagaac gctaacaaga tttgcaagaa actcaacctt aaacgagctt
     4021 cctagtacgc ttgcaatggc aataactgta accacagcaa ttgtcaactg catacctggt
     4081 tttgaaaggg caacaaattt gttgacaatg acaatcataa cgatagcaat cgtgattggg
     4141 tacaagataa ccagtactgg aattgagtac ttgataatcg catcaagacc caaattggca
     4201 atagcaaatc caatcaaggt aaaggctgtc gcataaacct tgtagctgat ttgtgggaag
     4261 cgctcattaa agaactcagc tgttgacaca atcaaaccaa cagtcgttgt gaagcaggtt
     4321 acggtaacca tagctgcaag gaagagttga gctgttgagc caaagatttc ttgagtggct
     4381 tgtgacaaga tgtaaacacc tggtgttcca cccttcatcg cttcagctgg tactgggaaa
     4441 tgatttccaa gaaaacctaa accgatgtaa agagcgctga aggcaagggc aacaacgata
     4501 ccaacaaccc aaatagttga aatgtattct ttcttacttg aaaatccaag ttgtttcaag
     4561 gtttgaactg cgattacgct aaaggccact gaggcaaggg cgtccaaggt attataacct
     4621 tctaggaaac ctgtaccaaa ggcagaagct tgataagcag ctgaagcagc ttgaggactt
     4681 gttccaccat atttgatagc tcccagaacg accaagataa caatcaaaat tgcaaagact
     4741 ggcgttaaaa tacgtccaat gcggtctaag atttttgatg gattaagcga aatcaaatag
     4801 gctgccgcaa aatacagaac cgtaaataca atcaagccaa gtcctttatt tgcatccgac
     4861 aaaagggggc taatccctac ttcgtaagct gttgtagcag tacgtggggt agcaaagaat
     4921 ggaccgattg acaagtaaag aactgagagg taaagagtcg caaaccaagg cgctatcttc
     4981 gttgaaatct cgtagatata tcctttagga tttagcgttc caataataag ggtcaagacg
     5041 gcgataccaa cgcctgaaaa gacaaaacct gcgatggcag gaagaaaatg ttctccagat
     5101 agagcaccta gagaaggcgg aaaaatcaag ttccccgcac caaaaaatat tccaaacagg
     5161 agcaaacctg ttagggtacc ttttttagcc ataaaaatct ccttcatatt tataaaatac
     5221 tgatctagta taccacgatt aggagtctga aaaaagcatc acgaggcaaa tattaaatgt
     5281 tttcaatttt cttaaaaata agaattatct aaaaataaaa ctctcctacc cagtccacac
     5341 caagtaggag aaatgcaaaa ttttaaagtt cttcaaaggc taacatgcca ccctggacat
     5401 tgataacatt ataaccttgt tctaatagga attggcaagc acgcgccgat ctcattccag
     5461 atttgcaaat aatataatgc aagcgatctt tgtccaatta atcataacta tcagccaatt
     5521 gactaagcgg taggttgtgg gcaccttcta aatgaagagc tgcaaactca tccacttctc
     5581 tcacatccac tagagaaagc tggtcgtttt ggtaaagctg gtaaaatgcg tcaaaggcta
     5641 tttctttcat tctttttct
//
LOCUS       DAVBHS010000129         5595 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVBHS010000129 DAVBHS010000000
VERSION     DAVBHS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.129"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(18..314)
                     /locus_tag="VQ651_001712"
     CDS             complement(18..314)
                     /locus_tag="VQ651_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HET2754674.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(467..923)
                     /locus_tag="VQ651_001713"
                     /pseudo
     CDS             complement(467..923)
                     /locus_tag="VQ651_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            1057..1884
                     /gene="xth"
                     /locus_tag="VQ651_001714"
     CDS             1057..1884
                     /gene="xth"
                     /locus_tag="VQ651_001714"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET2754675.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            1896..2039
                     /locus_tag="VQ651_001715"
     CDS             1896..2039
                     /locus_tag="VQ651_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET2754676.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEYQRSEENFLFLLK
                     SVN"
     gene            2039..2278
                     /locus_tag="VQ651_001716"
     CDS             2039..2278
                     /locus_tag="VQ651_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET2754677.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      2662..2757
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            3157..3738
                     /locus_tag="VQ651_001717"
     CDS             3157..3738
                     /locus_tag="VQ651_001717"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HET2754678.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            3738..5000
                     /locus_tag="VQ651_001718"
     CDS             3738..5000
                     /locus_tag="VQ651_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HET2754679.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(5087..>5595)
                     /locus_tag="VQ651_001719"
     CDS             complement(5087..>5595)
                     /locus_tag="VQ651_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HET2754680.1"
                     /translation="NHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLEAEGY
                     ILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFYSGSG
                     SKLNETARSYKMIAEEIKAIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNINDLE
                     HNILKNLK"
BASE COUNT         1606 a         1233 c         1023 g         1733 t
ORIGIN      
        1 aagaccccag gtttttctta ttcataagtt acgactgtaa cagcaccctt gtcatattca
       61 gcaataaaga tattggctac attgtcatgc ccttgtttgc tgaggttatc aagcaaccac
      121 tcttcgctac gaccaatcga ttccaagacg tctacttgaa tcacaccgtc agtcacaact
      181 ggatacttag gattttcatc tcccatttgc acaacgatga gttgcccatt ttgctcttgc
      241 acagctcgtt tgacttgctt catctggaaa atcccttggc tacgaagttt gagggctaca
      301 tccgatgcag acaaaccaac tgaacgacag gcttatgggt caatattccc atttttgata
      361 aggagagttg gcttaccatc aatcaaacgt ttcacaaagt gaacattgtt gttgagctat
      421 ttgagagtca agatcagttt gattaaaatt tcgatataat taagtgtcat ttgttcacat
      481 ccaccagttc aattgcgtct gtcttataca attcgacttt ctctaacagg tacttgtctg
      541 gctcacttcc gtttagggca cgataatagc gatctcccac cttgataagt gcgccatctg
      601 tgtttgtgga agtattaaca tggacttctg acttgtctac tcccaaatct ttggaaacaa
      661 cctcgatgaa atgaagtgaa gtttgaaatt tattgttaga aacttgattg ttttgataag
      721 tcgtgatgct gaccaaaagc atagctaaat aaggccagag ctgaaatcat gaccaactca
      781 cgaaatttgg ttcctttttt atcatggtat gccttgaaag caaaaaaaac tgtcacagct
      841 aatagaacaa tcccaaagcc aaccatgata ccattttgct gaccgatttg gctgagtaca
      901 tagtcataag agtagaattt catgtatttt cactcccttt cataaaatca ttcttaatta
      961 taaacgaaag agagtaattt ttcaaaccat acgttgggga aatagatctt tttttggtat
     1021 aataaaatct ataatctgaa tgaaaaaggt aactttatga aacttatctc atggaatatt
     1081 gattccctaa acgctgccct aactagtgac tcagctcgtg ccaaattgtc ccaagaagtc
     1141 ctacaaacct tggtcgctga aaatgctgat atcattgcta tccaagaaac caagctttct
     1201 gccaaaggac ctacaaagaa acacgtggaa attttagaag aactcttccc aggctacgaa
     1261 aacacgtggc gctcttccca agagcctgcc cgtaaaggct atgctggaac catgttcctt
     1321 tataagaaag aacttacacc cactatcagc ttcccagaaa tcggtgcccc ttctaccatg
     1381 gacttggaag gccgtatcat cactctagaa tttgatgcat ttttcgtaac ccaagtttac
     1441 actccaaacg ctggtgacgg tctcaaacgc ttggaagaac gccaagtctg ggatgtcaaa
     1501 tatgcggagt atttggctga actagacaaa gaaaaaccag tccttgcgac cggtgactac
     1561 aacgtagccc acaatgaaat cgaccttgca aatcctgcta gcaaccgccg ttcacctgga
     1621 tttactgacg aggaacgtgc tggatttacc aacctattgg caactggatt tactgatacc
     1681 ttccgccatg ttcatggcga tgttcctgaa cgctacactt ggtgggcaca acgcagcaaa
     1741 acttctaaaa tcaacaatac aggctggaga atcgactact ggctcacaag taaccgtatc
     1801 gctgacaagg tcactaaatc tgatatgatt gactcaggtg ctcgccaaga ccatacaccg
     1861 attgtcttgg aaattgactt gtaaggattt tctatatgga ctacaatgcg gtcattcccg
     1921 agtttcttgt atcgaatatc gaacagtccc ggtccttcta ctgtggttta ctgggtttta
     1981 ggattgaata ccaacgttca gaagaaaact ttctctttct cttgaagagt gtcaactaat
     2041 gttagaggag ggaacaaagg atcaactagc tgaactgacc tacccctttg gtcgtggagt
     2101 caatctctcc tttggcataa aggatgttcc aaaactctat caaaaagtga tggaggcaaa
     2161 ctatcctatt tatcgtccct tgaccaaaag aaagtttcgt gtcagtgatc cctatatcta
     2221 tcctcataaa tttgcagttt tggatccaga tggctatttt ttaagattta gcgagtagaa
     2281 taataaaaat tgggaaaagt ctttaaaagt tagaaaaagg catattgtca ggtgtctaaa
     2341 aacttgatag tatgcctttt atgatggaaa aatcttctaa tactcttcga aaatctcttc
     2401 aagccacgtc aacgtcgcct taccgtaggt atataatact gactctgtca attctatcca
     2461 caacctcaaa acagtgtttt gaggttgtgg atagcttcct agtttgctct ttgattttca
     2521 tttagtataa atgttagctt acaatggagt ttttgttcac cctctcttct gtttgttcgt
     2581 tttttaaacc aaaacactaa caaaatagtt gatttctaaa gttatttagt gtaaaataaa
     2641 aacattggct caagcctatt taaaattgaa tatcgtttta cttgtttatg tcgtgaattg
     2701 gcacgacgtt tctacaaggt gccggaacac ctaacaataa gtaagtcagc agtgagatat
     2761 ggccgttttt gccatgccta tttgggggac ttacttagag attaagtagg tcttttttga
     2821 atatttttaa tatttccctt aattagtgcg gacgggaggt gaaattatcc tgtgaataat
     2881 ttcaggaatc cagggaattt catccctaat ttttgagccc ctagctcttt catttctagg
     2941 cttaggctaa aatagttccc cgaactattt tactctcaaa aatcccgtca tagacaataa
     3001 caagattatt gtctatgata ccactacaac gggaaatatt tgataagact cgcttcgctc
     3061 gtaatagttt tatcaaatat atagcattag caaaagggat cactatagaa acatttaaac
     3121 aacaagtaaa ttaactttta gactttagga ggtcttatga aattattaga agagcgcatc
     3181 ctcaaggatg ggcatatctt gggtgataac atcctcaagg tagattcctt tttaacccac
     3241 caagttgact ttagcttgat gcgagagatt ggtaaggttt ttgcggaaaa atttgctgct
     3301 gccggcatta ccaaggtcgt aaccattgaa gcgtcgggta ttgccccagc cgtttttaca
     3361 gctgaagcct taaacgttcc catgattttc gccaaaaaag ctaagaacat caccatgaac
     3421 gaaggcatct taactgctca agtctactcc tttaccaagc aggtgaccag caccgtttct
     3481 atcgctggaa aattcctctc accagaggac aaggttttga ttatcgacga tttccttgct
     3541 aatggccaag ctgctaaagg cttgattcaa atcatcgaac aggccggtgc cacagtccaa
     3601 gctatcggta tcgtgattga gaaatccttc caagatggcc gtgatttgct tgaaaaagca
     3661 ggctaccctg tcctatcact tgctcgtttg gatcgttttg aaaatggtca ggtcgtattt
     3721 aaggaggcag atctctaatg caaactcaag aaaaacactc gcaagcagcc gttcttggct
     3781 tgcagcactt actagccatg tactcaggat ctatcctggt tcccatcatg attgcgacag
     3841 cccttggcta ttcagctgag cagttgacct acctgatttc tacagatatc ttcatgtgtg
     3901 gggtggcaac cttcctccaa ctccaactca acaaatactt tgggattgga ctcccagtcg
     3961 ttcttggagt tgcattccag tcggtcgctc ccttgattat gattgggcaa agccatggta
     4021 gtggcgctat gtttggtgcc cttatcgcat ctgggattta cgtggttctt gtttcaggca
     4081 tcttctcaaa agtggccaat ctcttcccct ctatcgtaac aggatctgtt attaccacga
     4141 ttggtttaac cttgatccct gtcgctattg gaaatatggg aaataacgtt ccagagccaa
     4201 ctggtcaaag tctcttgctt tcagctatta ctgttctgat tatcctcttg atcaacatct
     4261 ttaccaaagg atttatcaag tctatctcta ttttgattgg tctggttgtt ggaactgcca
     4321 ttgctgctac tatgggcttg gtggacttct ctcctgttgc ggtagctcca cttgtccatg
     4381 tcccaactcc actctacttt gggatgccaa cctttgaaat ctcatctatt gtcatgatgt
     4441 gtatcatcgc aacggtgtct atggttgagt caactggtgt ttatctagcc ttgtctgata
     4501 tcacaaaaga tccaatcgac agcacgcgcc ttcgcaacgg ttaccgcgca gaaggtttgg
     4561 ccgtacttct cggaggaatc tttaacacct tcccttacac cggattttca caaaacgttg
     4621 gtttggttaa attgtcaggc atcaaaaaac gcctgccaat ctactacgca gctggtttcc
     4681 tggttctcct tggactgctt cctaagtttg gtgcccttgc ccaaatcatt ccaagctccg
     4741 tcctcggcgg tgccatgctg gtgatgtttg gttttgtatc tattcaaggg atgcaaatcc
     4801 tagcccgagt tgactttgct aacaatgaac acaacttcct tatcgcagct gtttcaatcg
     4861 ctgcaggtgt cggtctcaac aacagtaatc tctttgtcag catgccgaca gccttccaaa
     4921 tgttcttctc aaacggaatc gtcgtagcca gcctactcgc cattgtcctc aatgccgtat
     4981 taaatcataa aaagaaataa gaaaaagagg tgcgagcctc ttttttgttt atcacaaatt
     5041 gattataaat acaatcaatc ccaattccca tctaattgaa ttggacctat ttcaaattct
     5101 ttaaaatatt atgttccaaa tcgttaatat tatataagaa tggtaaaata tcaaatgttt
     5161 ctcgtaaatt attcttagcc ttataccaac cttgtccatc agtaatccac ataaactcaa
     5221 cattaggaat agctttaatt tcttcagcaa tcattttgta agatctagcc gtctcattta
     5281 attttgaacc acttcctgaa taaaaattaa cctcaatcag ataaagcact tgttcatttt
     5341 ttatgacaaa atcaaaacgt ttcacagtat tcccatcatt cgtaatagca gataaatcga
     5401 cagaaaaaat ttcctctatt tcattttgtt caatctcttt aaataaattc tctcctaaaa
     5461 tatacccttc agcttcaaga taactttgaa caatattttc catcgcatct ccagtccgat
     5521 ttttgcgccc attagtatcc ataccaacct caacacctgt aacgtagtca actaaatttg
     5581 atactaaatg atttt
//
LOCUS       DAVBHS010000130         5459 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVBHS010000130 DAVBHS010000000
VERSION     DAVBHS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.130"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            98..1591
                     /locus_tag="VQ651_001720"
     CDS             98..1591
                     /locus_tag="VQ651_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HET2754681.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1604..2650
                     /locus_tag="VQ651_001721"
     CDS             1604..2650
                     /locus_tag="VQ651_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2754682.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     LQLDELNLLVKSRFNEMFGDVILNEKEWKVSKWNEILTIRNGKNQKQVEDADGKFPIY
                     GSGGIMGYAKDWIVKKNSVIIGRKGNINKPILVRENFWNVDTAFGLEPVLEKINSEYL
                     FYFCQLYNFEKLNKAVTIPSLTKSDLLNISIPLPPLALQNEFADFVALVDKSQFACEI
                     AIKVWRNSLKFSII"
     gene            2680..5376
                     /locus_tag="VQ651_001722"
                     /pseudo
     CDS             2680..5376
                     /locus_tag="VQ651_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT         1804 a          847 c         1135 g         1673 t
ORIGIN      
        1 ttcaggtgac gcatagatat agttaattga agctttgttt gaaatctgat aaaataatga
       61 tattactaag ttttaaaaac taaagaaaag ggaagatatg attacaggcg aattaaaaaa
      121 taaaatcgat cagctgtggg aaattctttg gacagaagga aacgcaaatc ctttaacaaa
      181 tattgaacag ttgacttatc tcttatttat gaaagatttg gatagtgtcg agcttggacg
      241 tgaaagtgat gctgaatttc tagggattcc ttatgaggga gtttttccaa aagataaacc
      301 tgaataccgt tggtcaactt ttaaaaatat aggagatgct caggaagttt atcgtttaat
      361 gactcaggag atttttccgt ttattaaaaa tctcaagggg gatacagatg atacagcctt
      421 ttcacgatat atgcgagaag ctatttttca aataaataaa cctgctacgc ttcaaaaggc
      481 aatttctatc ttagatgttt ttccaactag gggattagat gtagattttg ataatgacaa
      541 acaaagtatt actgatatcg gagatatcta tgaatatctg ttatcaaaat tgtcgaccgc
      601 aggtaaaaat ggacagttcc gtacacctcg tcacatcatc gatatgatgg ttgagttgat
      661 gcaaccgact atcaaagata tcatctcaga tcccgctatg ggttctgctg gcttcttagt
      721 atctgctagc cgttacttaa agcgtaagaa agatgaatgg gaaaccaata cagataatat
      781 caatcatttt cataatcaga tgtttcatgg aaatgatacg gatacgacta tgttgagact
      841 tggggcgatg aacatgatgc tacatggagt agaaaatcca caaatcagtt accttgactc
      901 gctgtctcaa gataatgaag aagccgataa atatactttg gttttagcaa atcctccttt
      961 taagggctca cttgactaca attcaacctc taatgacctt cttgcaaccg taaaaaccaa
     1021 aaaaacagaa ttactctttc tttctctttt cttgcgaact ttaaaaccag gtggacgagc
     1081 agcagttatc gtacctgatg gtgtcctttt tggttcgtct aaagctcata aaggaattcg
     1141 tcaggaaatt gtagagaatc ataagcttga tgctgtaatc tcaatgccta gtggtgtgtt
     1201 caagccttat gctggagttt caactgccat tctcatcttt acaaaaactg gtaatggtgg
     1261 tactgacaaa gtctggtttt acgatatgaa agcggatggt ttaagtttgg atgataagcg
     1321 acaaccgatt agcgacaatg atattccaga tattatcgaa cgctttcatc atcttgaaaa
     1381 agaagcagaa cgtcagagaa cggatcaatc tttctttgtt ccagttgctg agataaagga
     1441 aaatgattat gatttgtcta tcaataaata taaagagatt gagtatgaaa aagttgagta
     1501 tgaaccaaca gaggtcatct taaagaaaat caatgattta gaaaaagaaa ttcaagctgg
     1561 cttggctgaa ttagaaaaat tactcaagta gagaggtggc tgtatgaaaa aagtgaagtt
     1621 gggggaagtc ttatctctaa aaaaaggcaa gaaagccact gtacttgctg aacaaacaac
     1681 tctaagccaa cgttatattc aaatagatga tttaagaaat aataataatt taaaattcac
     1741 tgaaagttta aatatgactg aagcactccc agatgatatt ctgatagcat gggatggagc
     1801 taatgcagga acagttggtt atggattatc gggagctgtt ggtagtacaa ttacggtctt
     1861 aaaaaagaat gagcgataca aagaaaaaat tatatcagat tacttgggag tctttttgga
     1921 aagtaaatcg cagtatttac gagaacattc aacaggtgca acaattcctc atttaaacaa
     1981 gaatatatta cttgatttac aattagaatt gctaggtatc gaagaacaag agaacattat
     2041 ctgtattctt aatacgatta aaaggcttat tactaaaaga aaattgcagt tagatgaact
     2101 aaacttgctc gtcaaatccc gatttaacga gatgtttggg gatgttattc taaatgaaaa
     2161 agaatggaaa gttagcaaat ggaacgagat tctaacaata agaaatggta aaaatcaaaa
     2221 acaggttgaa gatgctgatg ggaaatttcc tatttatgga agtgggggaa ttatgggata
     2281 tgctaaagat tggatagtga agaaaaattc agttattatt ggacgtaaag gaaatataaa
     2341 taaacctatt ttagttagag aaaatttttg gaatgtagat acagcatttg gattagagcc
     2401 tgttttagaa aaaataaact cagaatattt attctatttt tgtcaattat acaattttga
     2461 gaagttaaat aaggctgtaa caatacctag tttaactaaa tcagatttat taaatatttc
     2521 catccccctc cccccactag ccctccaaaa tgagttcgca gactttgtag ccctggtcga
     2581 caaatcacaa tttgcttgtg agatagctat aaaagtgtgg agaaatagct tgaaatttag
     2641 tataatatag ctaaactatt tgtttaaagt gagaaaaaaa tgggaaattt tagctttctt
     2701 ttaaaaaatg acgaatatga atctttttca aaaccttgca ttgaagctga gaatatgatt
     2761 gctacatcaa ctgtggctac tgcctttatg gcgcgtcgtg ctttagagca ggctgtccat
     2821 tggatatata gtcacgattc atatttagaa gctccctatc gtgctactct atcttcttta
     2881 gtatgggatg atgattttag ggatatcgta gattctgaac tccacaagca gatagttctg
     2941 ttgattcggt ggggaaacca tgctgctcat ggtggtgaaa ttaaggaacg agaagcgatt
     3001 ttagctttgc atcatttgta tcagtttgtt aattttatcg attattgtta cagcaatgag
     3061 tttgtggagc gttattttga tgagaagtgc ttaccacttt cagcaaacat caaataccga
     3121 gaaactccac aatctatgat aaagttacaa gacagtttac cagaactgcc tgattttcat
     3181 gaacagatgg ctgctcagtc cgtagaagtt caagagactt atactgaaaa acgtgagact
     3241 gcagcgcaac ggcaagatgt gcctttccat attgatcaat tatctgaggc agagacaaga
     3301 aagctcttta ttgatatcga tctccgttta gcaggatgga tatttgaaga aaactgtcgt
     3361 gttgagatag ccgttgatgg tctcaagcac ggttcaggaa ttggttactg tgactatgta
     3421 ctttatggta aaaatgggaa aattttagcg attgtggagg ctaaaaaagc ctctgtcaat
     3481 ccagaagtag gggaagtaca ggtcaaagaa tatgctgaag ctttggagaa acatatcggc
     3541 tatcagccaa tttgctttat tacaaatggg ttgaagcact atatacttga tggtccgaac
     3601 cgccgccaga ttgcaggctt ttactctcaa gaagaattgc aattagtgat ggatagacgt
     3661 catcttcaaa aaccgcttga ggatatttct agtaaaatta gggacgatat ttccgggcgt
     3721 cactaccaaa aacatgccat tgcaagcgtt tgtgaagctt tctctgatca tcgtagacag
     3781 gcacttttgg ttatggcaac tggggcgggg aaaactcgta cagcagtttc tctagttgat
     3841 atcttatcac gtcataactg ggtaaaaaac gttctcttct tagccgatag aacttccttg
     3901 gttaagcaag catatgattc gtttagaaaa ttactcccag atctttccgt ttgtaacttc
     3961 ttagaagata aagaaggagc tcaatcaagt cgaatggtct tttcaactta tccgaccatg
     4021 attggagcga ttagtggtca agaagaagta aatcaacgcc cttttactgt tgggcatttt
     4081 gaccttatca taattgacga atctcaccgt tctatttatc agaaatacaa gtccattttt
     4141 gattattttg atgcaaggat tgtaggctta acagctactc cgcgtcaaga tttagataaa
     4201 aacacctatg gattctttaa tttggagaat ggggttccaa catatgcata tgatttggaa
     4261 gaggctgtta aagacggata tttagtagcc tatcattcta tcgaaaccaa actgaaacta
     4321 cctacggatg gtctacatta tgatgatttg tccgaagaag aaaaggaaca ttttgatagc
     4381 aaatttgaag acgatagctg tgaaaaagat attgatggga gtgtgtttaa ttcctttatt
     4441 ttcaataaaa gtacagtaga aattgtttta aatgaactca tgacaagagg aattcagaca
     4501 gcctcgggtg atgaaattgg taaaactatt atttttgcta aaaatcatga ccatgcggaa
     4561 tatatcagag gtatttttaa caaccgctat cctgaaaaag ggagcgacta tgctcaggtg
     4621 attgattata gtattaagca ttatcagacc ttgattgatg attttaaaat taaggagaag
     4681 tatcctcaaa ttgcgatttc tgtcgatatg ttagatacag gtattgatgt accagaggtt
     4741 gttaatttag tcttcttcaa gaaagtacgc tctaaaacta agttttggca gatgattggt
     4801 cgaggaaccc gtctatgtaa agatttattt ggacctgagc aggataagga aaacttcttg
     4861 gtatttgatt atggggacaa ttttgattat tttcgtgcag atccaagaga tggagagggt
     4921 cgtcacattg tttcgctgac tcagcgttta tttaatatca aagtggactt gattcgagaa
     4981 cttcagggac tccaatacca agaagatcag tttgcgagag cataccgtca gcagcttgtc
     5041 tcggaacttc aaggtcgtat agagagctta aatgagttgg acttcagggt tcgtatggtt
     5101 ttagatacag tttatagcta taggaaattg gaaagttggc agaatctaac tgctgttaca
     5161 agtgaaacca ttcaaaaaaa tctctctccg cttttatttg atgaagataa agaagatgag
     5221 atggcgagga gatttgattt gtggttgctt catattcagt tggggcaact gacagctaaa
     5281 tcttccactg ttcatatttc ccaagtgatg aagacggcta gagctctttc tgctattggc
     5341 aatatcccgc aggtttttga gcaggctgaa attatggctc tttgtcaact gtagtgggtt
     5401 gaaaaaaagc taagcttgag aaaggacaaa ttttgtcctt tcttttttga tattcaggg
//
LOCUS       DAVBHS010000131         5297 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVBHS010000131 DAVBHS010000000
VERSION     DAVBHS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.131"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(79..2133)
                     /locus_tag="VQ651_001723"
     CDS             complement(79..2133)
                     /locus_tag="VQ651_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET2754683.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            complement(2145..2399)
                     /locus_tag="VQ651_001724"
     CDS             complement(2145..2399)
                     /locus_tag="VQ651_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754684.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERSVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            complement(2392..2949)
                     /locus_tag="VQ651_001725"
     CDS             complement(2392..2949)
                     /locus_tag="VQ651_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2754685.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQADLTNAQLILQGHETLDQFVIKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            complement(2933..3889)
                     /locus_tag="VQ651_001726"
                     /pseudo
     CDS             complement(2933..3889)
                     /locus_tag="VQ651_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            3981..4340
                     /locus_tag="VQ651_001727"
     CDS             3981..4340
                     /locus_tag="VQ651_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HET2754686.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            4551..5297
                     /locus_tag="VQ651_001728"
                     /pseudo
     CDS             4551..5297
                     /locus_tag="VQ651_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         1612 a         1165 c          984 g         1536 t
ORIGIN      
        1 tgaaactgac gaagtcagtc acatatataa tccaaggcga cgctgacgtg gtttgaagag
       61 attttcgaag agtattaact aatttcttct taccaattcc accatatcat acggcagggt
      121 attggcagct tccttcaagg aatagttctc taagttattt acattttgtc gtaatttctt
      181 ggcatactta gtcgtaatca atcgtttttc ttcgtattcg aaaatcaact tgcgctccag
      241 ataatagcct ctcagcattt catcgatatt gttgggtttg acacgattga taacccgttc
      301 gacaaaggca ccactgctga taatagctgt ttctcgaaga cgagactcct gcataaaact
      361 aatcaaagag cgtctgtaga ctcccttcag gttttccaaa ctttcaataa tcatctctgt
      421 attggcaaga tagagctctg caatttggtc ataatcaaga gcacggagac ggctttgctc
      481 cttgtccttc cagctacgga aggtctttcc aagagtaaaa acttcatgaa ggagaaaacg
      541 taaaatcctc aaggaaacaa gaaaataata ggtcagtctt gaggcaagtt tacgattgat
      601 tccttgttct atatttttca gataacgttg gtaaactcgg taagcacgat tgctaatgtt
      661 cccctcttca taggcctgtt ccaaaccatc actttcaata ctaagaatca agagtttcaa
      721 agcagcccaa tcttcttgat catcctggtt ttcttggctt aaaatgagat tttcaatacg
      781 tccatgataa ttgtcaatag ccgcatagag gggaagttta tttctggtgt cttccaactc
      841 tttttccaac tctagcgtta cttcattcaa aatggcgata tgcatgagat aatccttgct
      901 ttcttcctct tcatcagaaa gatgaggcaa gaccaagaga cctgttaaaa agctgacaag
      961 cgtcacacct gcaacaagga aaagcaaaag aggatactcc tgttctagat tacttggtat
     1021 caagagaatc gtagcaatcg acaccgttcc cttaacacct gaaaaggtca agagaaacat
     1081 gtccttcata tacttattta gctttttctt gaggcgtcgg gttctatagg cataatagcc
     1141 atagatcata ataaaacgaa tgacaaagag gacaaaggta agggcgatga gagatagcaa
     1201 taaaagtaga ggattataga ttggattggt caagataggt tctgctatca tttccaactc
     1261 catccctaaa atcacaaaga cagaaccgtt gagcataaag gtcactgtat gccagaccgt
     1321 ctcggtcacc gtatccactt gggcttcgag gagcgtgatt ttcttgaagc gacttgcctt
     1381 taaaattcca gcaactacga cggcaataat acctgaaaca tgaacttctt ctgccagaaa
     1441 gaaggtcact agaggcaaac tcaattctaa taaaagttca ctggcaatat ccgttgcgcg
     1501 cacacttagc aagaaggtat ggaggaagcg gttggtcatg gccgttaaaa atccaattaa
     1561 aaaaccgcct aggattgaaa agatgagcga actgctagct tgccccagag aaaaagctcc
     1621 agttgtccaa gctgtcaaag ctacctgaaa agccaccaaa ccagaagcat cattcaagag
     1681 tccttcgccc ttaagaatat tggacacgcg cttaggaaag ctaaaacgct ccgaaagaga
     1741 ggcaaaggcc accaagtccg taggaccaag ggctgcccca acagccaagc aagctgccaa
     1801 gggaaggctg aaccaaagaa gatgggccaa gccacccaaa ctcaaggtcg agataaaaat
     1861 cactggaaat atgagataaa caatgattcg ccagtgtttt aaaatagccg taacatctgc
     1921 ttcttcagcc tctcggaaaa gcaagggtcc gataaccagt gccaaaaaca actccgtatt
     1981 aaggtgaaag tcagtattgg gtaaaaagag accaatcaca attcccaaaa gaatctgcac
     2041 caaagggaga ggcaaaaagg gcaggagctt attggttgta cttgagacaa tcaaaaccag
     2101 taaaaatagg atgaggtaaa tcagtaattc cacgcacgtc ctccttaatc ttttttacaa
     2161 caggattcaa atatctcctt ctgctctttg attttttggt caatcttgga acagtctttg
     2221 tgctcaattt ttctctggca ccgttccatt tcaagagcaa ctaatttttt cttgatttta
     2281 agcatttttt tgctcatatg cgcttggtct agcacgccca ccgatcgttc gtggtgggtt
     2341 gattcaacaa aattctggcg catggcatcc agcttttcgt gtaggtattg tttatccatg
     2401 tctgtatctc tctaattttt caatcatcac taaaaacggc gggttgttga cttggtttaa
     2461 agttcggtaa atggcagctg tgtactcttg ttggttcaac tggatcacaa aatccaagac
     2521 agcatctctc tcgagatcgc ctccttcatg accatagtaa atcataatag caattcgtcc
     2581 acctttgaca agtaagccac atagcttttc taatgcctca atcgttgtct gcggtcgggt
     2641 gatgacagac ttatcagctg acggcaaata gcccagatta aaaatccctg ccttagcttt
     2701 tatcacaaac tggtccagtg tctcatggcc ttgcaagatt aactgggcat ttgtcaagtc
     2761 agcctgatgc aaacgctctt gggtcttttc caaggcttgc ttctgaatat caaaggcata
     2821 gacttgcttg gctagcttgg ctaaaaaaag cgtgtcatga ccatttccca tagtcgcatc
     2881 cactacgaca tcctcttttg tcacgacctc agccaaaaaa tcatgtgcca tctcaagtgg
     2941 tcttttcatt ttcaaactcc tgttttacag ccttgcatcc ttgaacactt ccacgacgtc
     3001 gcatctccat ctcaatgctg ttgagagctt cccatttatt gaggctccac ataggcccaa
     3061 gcagcatatc cctaggcgca tctcctgtaa ttcgatggat gacgatatgt ttgggaataa
     3121 tttccagttg gtcacagatg accctgacat attcgtcctg actcatcaat tgtaaacgcc
     3181 cctcatggta atctcgttgc atacgagtat ttgtcataag atggagcaaa tgcagtttaa
     3241 tcccttgaat atcgttatcc gtgacacaac ggcggacatt ttcaaccatc atctcatggg
     3301 tttcaccagg caaaccattg atcaaatggg aaacaatctc aatttttgga tactttctca
     3361 aacgcttgac cgtttccacc tacaattcat aagaatgcgc acggttaatc aggtcagagg
     3421 ttgcttcata agtagtttgc aagcccaatt caaccgtcac atgcatgcac tccgataact
     3481 cagccaaata ttcgatggtt tcgtctggta aacagtctgg gcgcgttcca atattgattc
     3541 ctaccacacc tggctcattg atagcctgtt cataacgctc tcgaataact tccacctttt
     3601 catgggtgtt ggtaaaattt tgaaaataaa ccagatactt ctgaacatct ggccacttac
     3661 gatgaataaa gtcaatttcc ttataaaatt gctcacggat aggcgcatct ggtgccacaa
     3721 tggcatctcc agaaccggaa accgtacaaa aagtacagcc tccatgagcc acagttccat
     3781 cacgattggg acagtcaaat cccgcatcaa tagggacttt aaaagtcttt tctccaaaga
     3841 gttttcgata ataatcattc aaggtattat aagattttat aactttcatt ataacaaaaa
     3901 tcacccacaa tctcaaaagc ctgactttcc tataaattcc tctgtttctc gtttccatta
     3961 gccttttttt atgatacaat atgggtatga ttttaatgaa attagcatct attttattat
     4021 tgatactgac cttagtcgtc tgcattatcc taaccaaact ttttagatta aaaaaactag
     4081 gacgaaactt tgcggatttg gcttttccag tcttggtatt tgagtattac ttgattacag
     4141 ctaaaacctt tacccataat ttcctcccta gactggggct agccctctcg atcctagcca
     4201 ttattctcgt ctttttcttc cttttgaaaa aacgcagctt ttactatcct aaatttatca
     4261 aattcttctg gcgtgcagga ttcttattaa cccttatcat gtatatagaa atgattgttg
     4321 aattgttctt aatgaaatag tcgaatccct aagcattttc tagggatttt tgctttctct
     4381 acaaaatagt atagacaata acactataca attttataca aagaaaagag tctgggacaa
     4441 tagtctctta tctccaaaaa aggcaacgga tttgccgttg cttttttgca tggttgcgat
     4501 agtcttggta aaatagaatt gcttaataaa ccatttagaa aggctatccc atgcatattc
     4561 actataacac aaatcaaaca actttaccac tagaaatcag ttccttcttg ccacaagatc
     4621 atctcgtttt tactattgaa aaagtggtga ataccttgga ggattgtcac ttccacgcct
     4681 tctatcatgc ctttgatcgc ccgtcttatc accctaaaat gcttgtatct actcttctat
     4741 ttgcctattc acaagggatt ttctctggtc gaaaaattga aaaatggaag agttagtgac
     4801 cttagattgt ttgtttattg acggaactaa gattgaagcc aatgccaaca agtatagttt
     4861 tgtgtggaag aaaacgacag agaaattctc cgccaaactt caagaacaga tacaggtcta
     4921 ttttcaagaa gaaatcactc cccttctgat taaatatgcc atgtttgata aggaacaaaa
     4981 gagagggtat aaacagtcag ctaaaaactt agcgaattgg cactataatg acaaggagga
     5041 tagctacaca catcctgatg gctggtgcta tcgttttcac catatcaagt accagaaaac
     5101 acagacagac tttcaacagg aaatcaaggt ttactacgct gatgaacctg aatcagtccc
     5161 tcaaaaggga ctgtatatga acgaacgcta tcaaaacttg aaagctaaag aatgtcaggc
     5221 gcttttatct ccccaaggta gacagatttt cgctcaacgc aagattgatg tggaacctgt
     5281 ctttgggcag ataaagg
//
LOCUS       DAVBHS010000132         5113 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVBHS010000132 DAVBHS010000000
VERSION     DAVBHS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.132"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..936
                     /gene="trpB"
                     /locus_tag="VQ651_001729"
     CDS             <1..936
                     /gene="trpB"
                     /locus_tag="VQ651_001729"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET2754687.1"
                     /translation="GQVWLAKRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGE
                     EDVKRQALNVFRMELLGAKVEAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGP
                     HPFPEIVRDFQSVIGREAKQQYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVA
                     MYGAEAAGFGVDTEHHAATLTKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGI
                     GPEHSHYHDIKRASYVPVTDEEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGP
                     EKSMIVCLSGRGDKDVVQVKDRLEADAAKKGEAHA"
     gene            929..1705
                     /locus_tag="VQ651_001730"
     CDS             929..1705
                     /locus_tag="VQ651_001730"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET2754688.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(1677..1778)
                     /locus_tag="VQ651_001731"
                     /pseudo
     CDS             complement(1677..1778)
                     /locus_tag="VQ651_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            2007..2738
                     /locus_tag="VQ651_001732"
     CDS             2007..2738
                     /locus_tag="VQ651_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754689.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            2753..3904
                     /locus_tag="VQ651_001733"
     CDS             2753..3904
                     /locus_tag="VQ651_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET2754690.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(3956..4615)
                     /locus_tag="VQ651_001734"
     CDS             complement(3956..4615)
                     /locus_tag="VQ651_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET2754691.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTPLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCVIIFGKLLL
                     V"
     gene            4685..>5113
                     /locus_tag="VQ651_001735"
     CDS             4685..>5113
                     /locus_tag="VQ651_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2754692.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLPLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYS"
BASE COUNT         1693 a          875 c         1125 g         1420 t
ORIGIN      
        1 ggacaagttt ggcttgccaa acgcatgggt aaaaagaaaa ttatcgcaga aactggtgct
       61 ggtcagcacg gtgtggcaac tgcaactgct gcggccctct ttaacatgga atgtaccatc
      121 tacatgggtg aggaagatgt caaacgccaa gccctcaatg tcttccgtat ggagcttttg
      181 ggagctaagg tcgaggccgt gacagatggt tcgcgcgtgc tcaaggatgc ggtcaatgca
      241 gcccttcgtt catgggtggc taatatagat gatacccact atatccttgg ttctgccttg
      301 gggcctcatc catttccaga aattgttcgt gacttccaaa gtgtcattgg tcgagaggct
      361 aaacaacagt accgtgactt gacaggacaa aatctgccag atgccctagt agcctgtgtt
      421 ggtggtgggt cgaatgccat cggtctcttc catccctttg tagaagatga gtcagtagcc
      481 atgtatggag ctgaagcggc tggatttggt gtggatacgg agcaccacgc agctaccttg
      541 accaagggtc gtccaggtgt ccttcacggt tccctcatgg atgtgctcca agatgcccat
      601 ggtcaaattc ttgaagcctt ctctatctca gcaggtttgg actatcctgg tatcggtcca
      661 gagcattctc actaccacga tattaaacgt gccagctatg tccctgtgac agacgaagaa
      721 gccttggaag gattccaact cttgtctcgt gtggaaggga ttatcccagc tttggaatct
      781 agccatgcta tcgcctttgc ggtgaaattg gccaaagaac ttggaccaga aaagtctatg
      841 attgtatgcc tatcaggtcg tggggacaag gatgtggttc aagtaaaaga ccgcttggaa
      901 gcagatgcag caaagaaggg agaagctcat gcctaagaca ctaacaaaaa aattaaatgc
      961 tattaaagca gctggaaaag gaatttttgt tccctatatc atggctggtg accacgaaaa
     1021 aggtttggat ggtcttgctg aaacaattca ctttttagaa gatttgggtg tctctgcaat
     1081 tgaagtgggt attccctttt cagaccctgt tgcagatgga cctgttatcg aagaagctgg
     1141 cttgcgcagt ttagcccacg ggacctctac ccaggcttta gttgaaacct tgaaaaccat
     1201 tgaaacagag attccactgg tcatcatgac ctacttcaac cccctctttc agtacggtgt
     1261 ggagaacttt gtcaaagatc tggctgatac agctgtgaag ggcttgatta tcccagacct
     1321 gcctcatgaa catgccaact ttgtagaacc atttttggca gatacagata tcgccttgat
     1381 tcctttagta agcttgacca caggaattga gcgccagaaa gagttgattg aaggggcgga
     1441 gggattcgtc tatgccgttg ccatcaatgg ggtaacaggg aaatcaggca attaccgtgc
     1501 agatttggac aagcacttgg cgcaacttca tcaagtggcc gacatcccag tcttgacagg
     1561 ttttggtgta tctagtcaag ccgatctgga acgcttcaat gcggtgtcag atggtgttat
     1621 cgtcggttcg aaaatagtaa aagctctcca ccaaggagag ccgattcagg actttatcaa
     1681 acaagcagta gcttaccaaa aataattaaa caagtagcaa gtaagaggaa aggcacgaaa
     1741 ggaagtcttt cctttttctt ttgcaggaga aaggctagtt ttatagtaaa ttgaatctgg
     1801 aatattacac tacggttcct aaaatatttt tagaaagtga tttgaatttc ccaataaatt
     1861 tgttcagttt ttaattgatt ttactataga agatataatt ttctaaatta ctctgacaca
     1921 gaactttaag aaaaagctaa ttattgtagt aatttcttta agaaaatgat ataattcatt
     1981 aaaaagtatt tggaggagga agaattatgt tgaatcagga tctctttgat tcgcttgagg
     2041 cacaaaaaat tgtagatact ttgatgaaag ggcaaaaaga ttatgtagat gaacgtctag
     2101 aaaaaagaga gacaatgata gtgtcgaatg gttatgcatg gacgcgacct aatcatattg
     2161 atactgcatt tgcatcagca gatttgtttg agtataaatt acaattagca ggacagactt
     2221 ggggatattt agaatttgaa acaaacacag aaaaatatgg gaaagtattg ttaattataa
     2281 agggtaagaa gcgacttacg aaccaatttc ctttggtaca aaaaaataag agtggctact
     2341 tatttgaata tgctcagatg aatacacttt atcttaatca acattcttcc tacaaaaatg
     2401 atgaagatag tcattccttt ccaattcaga tggagttagt ttctgatgaa atgattcaag
     2461 aaattgaaca agctactaaa aattcgaata tcgaaaaatt tatgatttta acttatgagg
     2521 cggactcaga aaacaatatt atatctgtag atgttgttat gcctgatgca cgaactggtc
     2581 agttacactt gattcaagat ttgtctgagt atattcaatc aagttcgtac catttcgaag
     2641 aagctaaata tcaagatatt cctaattttt cagaattatc ggaaacagaa gattttgaaa
     2701 ttattccaag aatagaaaaa caagaaggtc aaaagtagta aggagtatgt aaatgtttaa
     2761 tggtcgggta ttgaaagaat tacggctgtt aaatggttta agtagagcag aattagctca
     2821 gagaattaat ttaacggaac aagccatttg gcagtttgag tccaacgaaa cgaaacctaa
     2881 attatcaacc aaaatgcatt tggccaacca atttcatgtt gatttaactt attttgaaca
     2941 ggaagaagag agcattcgat ttgattcttc tgtaattgcc tttagaaatg cagacctagc
     3001 aacccggaaa acaatagata ttcaaactat gtatttacat aaggtagata gtttgattga
     3061 ttattttgaa agttttgtaa ttatacctaa tattacaatt catgacctaa gtaatgtagt
     3121 gagtgaatct tatcataagg gagaatccat tgaggaattg gctctttatg ccagggaaaa
     3181 attaggtatt tcaaaagata atcatgattt gctttataaa ttagaacgtt caggcatcta
     3241 tatcgtggaa cgattaatta atggccaagc tgatgcttat agcgcatggt caaaattggg
     3301 aagaccttat attgtgttgg gaacgaataa atcatctgta cgtcgaaatt ttgatttagc
     3361 tcatgagcta ggacatattc ttttacataa atataaagat atgaatgaag atggcgatcg
     3421 tttggagcaa gaagcaaatt attttgcatc atgtttttta ttgccaaaag aagagttttt
     3481 agtcaaattt gaagagaggg ttggcaagcg tgtcagcaat cctgatagtt atattttatt
     3541 gaagtcggat ttgaatgttt cgatacaggc tttagagtat cgagctttta agttaggatt
     3601 attgactcca aagcaacatt cttactttta tcgtcaaatt gcgcaaaaag gttacaaaat
     3661 gattgaacct ttggatgatc aaatttttgt taaaaaacca agcaaagtaa agagtattct
     3721 ggacgtcgtt ttgagtaatc atctagtcag tctagcgact ataatgtcta aacaaagtat
     3781 tcgtttacag tttataagcg aaatattttc agtcgaaatg aaattttttg atcagtatca
     3841 agaagataga agaacagatc gatttgataa catcatccct ttgtacaaaa gaaataattt
     3901 ataatttaac tgtgaaataa tttcatggag tacatatggc agaaattgga atttatcaaa
     3961 caagtagtag cttaccaaaa ataatcacac aagtagcaag taagaggaaa ggcacgaaag
     4021 gaagtctttc ctttttcttt tgcaggagaa aggccaggat acccgtcgca gaagcgaact
     4081 gaatcaagat cagtaactcc gttacgctaa agacgagagc acaagaagct aaaaagagga
     4141 aatcccctgc acccatgcgg atatcaataa aatgagccaa aattccaagt atgaggaagg
     4201 agaccatgac cagattccag ccagaggaag ctattaggat taggtggaaa gtcatccaga
     4261 ccagtaaggg atattcctga tggtgaaagt cgtatatacc caaggtcaaa ccagcggtga
     4321 ttaggacgac ttgccccaag gaaagcaatt cccaagagta aagcagaaag aggagtccta
     4381 agcctagttc aaagagggca taccagacag gatagcgaac tttgcagtag cgacagcgaa
     4441 agcgattgaa gacctgtgag agaatcggaa tcaaatctaa gggacgcaag ggagtctgac
     4501 aggaatcgca gtgactggct gaactgataa tggattgctc tggaaaacgg tcaatgacca
     4561 aaccaagaaa ggaagcgaga atgctcccga cgagaaaaaa ataaaaatca atcatactta
     4621 tctattcgta aaaaatggga gaaatagtat aatggagaaa catagataag atggtgagga
     4681 aaagatgaca attcgttttg aagaaaaggt gagtatagaa aacgctcaat ttgtatgcca
     4741 gtggtccaac tccctaggca aatcctttca agaacaatgg atgggaccaa ggattccttt
     4801 tctactgccc cttcaagcct tggaaggagt cttttcaatc tttgatgaac aagagtttgt
     4861 gggacttatc cagaaaatca ggctagaaga cagcaatctt catatcggga gattttttat
     4921 caacccccag aaacaggagc aagacttagg tagccaggct ttaaggaaat ttgttagttt
     4981 ggcctttgaa aatgaagata tagatagtat ttctctaaat gtcttcgagg caaatcaaag
     5041 agctcagaat ctttaccaaa aagaaggatt tgaaatcgtt caaatggttg aagcacctgt
     5101 acgaaaatat agt
//
LOCUS       DAVBHS010000133         4987 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVBHS010000133 DAVBHS010000000
VERSION     DAVBHS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.133"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(44..283)
                     /locus_tag="VQ651_001736"
     CDS             complement(44..283)
                     /locus_tag="VQ651_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET2754693.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(303..575)
                     /gene="rpsP"
                     /locus_tag="VQ651_001737"
     CDS             complement(303..575)
                     /gene="rpsP"
                     /locus_tag="VQ651_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET2754694.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            745..954
                     /locus_tag="VQ651_001738"
     CDS             745..954
                     /locus_tag="VQ651_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754695.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            1433..1654
                     /locus_tag="VQ651_001739"
     CDS             1433..1654
                     /locus_tag="VQ651_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754696.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            1737..2540
                     /locus_tag="VQ651_001740"
     CDS             1737..2540
                     /locus_tag="VQ651_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET2754697.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQSSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(2596..4137)
                     /locus_tag="VQ651_001741"
     CDS             complement(2596..4137)
                     /locus_tag="VQ651_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2754698.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMEMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DLPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(4139..4621)
                     /locus_tag="VQ651_001742"
     CDS             complement(4139..4621)
                     /locus_tag="VQ651_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET2754699.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVYFCFTPQEQSTVGVGTPDIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(4621..>4987)
                     /locus_tag="VQ651_001743"
     CDS             complement(4621..>4987)
                     /locus_tag="VQ651_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HET2754700.1"
                     /translation="KLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAELLKNI
                     KKSSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGTDIDAACGQ
                     LLFNTMKRDRQKAVAAVNP"
BASE COUNT         1529 a         1058 c          935 g         1465 t
ORIGIN      
        1 cttctattcc acacaaaaaa gcgagacatc cgtcccgccc ttcttatttt tcgtcaataa
       61 cgattcttac ttttttgtat tcagttggga cagagtagac aatcgttctt atcgcagaaa
      121 tagtgcgacc cttacgaccg attacacgac ccacatcgct ttgatcaaga ttcaaatgat
      181 attccaaaaa ttctggtgta tcctcaatct tgatagttaa ggcatctggt tgtgaaatta
      241 agggtttcac aatcgcaata atgagatttt caatcgtatc catctgtcaa cctactttaa
      301 acttattttg aaaatttaga atcgtggaat tttttcaata cgccttcttt tgaaaggatg
      361 ttacgtactg tgtctgaagg ttgagctcca ttagccaacc atgcaagaac gcggtcttct
      421 ttcaaagtta cttggttttc agcaacaagt gggttgtaag ttccaactgt ttcgatgaaa
      481 cgtccgtcac gtggtgaacg tgaatctgct acgttgatac ggtagaaagg ttttttctta
      541 gaacccatac gagtcaaacg gattttaact gccattttta aagtctcatt tctttaattt
      601 tttatttcgg tgaaatagct gagctattta gcacatgttc tattatagca gatttctggc
      661 atgtgtcaag aaaaaaactt gacagactat aaatttctta aagtatttag aaatttgtta
      721 gaataggaaa taaatgtttg aaagatgcta tccgtgaata ctattttaga aaaattttat
      781 aaagaacacc aagtcaaacc cttcatatcg cctgaacgag agttggatac ttggcttcta
      841 agtcccaagc ccgttcccaa gcgaaatatg gacttattag tagatgattc actagcagga
      901 gatatcattt tactatggag aatccagttt ggtactttta ccactgaaac atgattttaa
      961 aaactcccat tcgaatatga ttcaaaattc gcttagaatc aatgttttcc cgctttttca
     1021 aaaaataaat tttcatctaa tttaaataac tttcaattaa gtgtgaattt tgcttaaaat
     1081 cattaaaaag caccccatgg tgtgaaccat gaagtgttgt aaatgctgtg ttgtagctag
     1141 ttcttagcgt ttagagaact ggctagcttt acgagctttc ttaagatctg gtttccaaaa
     1201 tactttgagt acacttatgg tattgatgcc cctaaacatc taaaaactct ggtagaaaaa
     1261 gggtatgctc ttgtcgaaac ttcttttgac tcacttgacc atctaaataa taccatgaaa
     1321 aagaatatcc tgaagaagaa gggagttact ggactttcta aaatgaaatc ttgcgacctg
     1381 gatcaagccc ttcatgagca tttttcagaa gaagaattag ctggtcactt tcatgtcctt
     1441 ctatggactt ttttacaatg gcattgctat cacacccaat acctatctaa gcgcctggtt
     1501 cgtaaacttt attgcagctc ttcctctaaa tttcctaatt gttgaaccaa ttgcccgttt
     1561 tatactaagt tcttttcaga aaccatttac tggggaagaa gttgaagaag ttgaagattt
     1621 tcaagatgat gatgaaatcc caactattat ctaagccagt tctgtaaact actaatattt
     1681 gaaatccact tctttttagg gtacaatggt ataaatgaat ttttgagagg atcagaatga
     1741 aaaaactagc aacccttctt ttactgtcta ctgtagccct agctgggtgt agcagcgtcc
     1801 aacgcagtct gcgtggtgat gattatgttg attccagtct tgctgctgaa gaaagttcca
     1861 aagtagctgc ccaatctgcc aaggagttaa acgatgcttt aacaaacgaa aacgccaatt
     1921 tcccacaact atctaaggaa gttgctgaag atgaagccga agtgattctc cacacaagcc
     1981 aaggtgatat tcgcattaaa ctcttcccta aactcgctcc tctagcggtt gaaaatttcc
     2041 tcactcacgc caaagaaggc tactataacg gtattacctt ccaccgtgtc atcgatggct
     2101 ttatggtcca aactggagat ccaaaagggg acggtacagg tggtcagtcc atctggcatg
     2161 acaaggataa gactaaagac aaaggaactg gtttcaagaa cgagattact ccttatttgt
     2221 ataacatccg tggtgctctt gctatggcta atactggtca accaaacacc aatggcagcc
     2281 agttcttcat caaccaaagc tctacagata cctcttctaa actccctaca agcaagtatc
     2341 cacagaaaat tatcgaagcc tacaaagaag gtggaaaccc tagtctagat ggcaaacacc
     2401 cagtctttgg tcaagtgatt gacggtatgg atgttgtgga taagattgct aaggccgaaa
     2461 aagatgaaaa agacaagcca actactgcta tcacaatcga cagcatcgaa gtggtgaaag
     2521 actacgattt taaatcttaa aaaccaaaaa aatacagtat ccacattcgg tactgtattt
     2581 cttttactct cattcttaag ttaaattatt aaaatcccat atttggtcta tccagccttc
     2641 ataaaagtct ggctcgtggc agaccataag gatagatccc ctatattctt tgagagcgcg
     2701 tttgagctca tcctttgcat ccacatccaa atggttggtc ggctcgtcca gcactaaaac
     2761 gttgttttca cgattcatca agagacagaa acgaaccttg gcttgctctc cccctgataa
     2821 tacttgaatc tggctttcaa tatgtttggt tgtcaaacca caacgggcaa gggctgcacg
     2881 gacttctgct tgattaaggg caggaaaggc attccagaca gcttcaagag gagtttggcg
     2941 attaccgcct tctacttcct gctcaaaata accaagttct aaataatctc cacgctccac
     3001 ttccccagcg attggcgaga taatgcccaa gagactcttc aagagagttg tttttccaat
     3061 accattagca ccaataatcg caaccttttg attgcgttcg aaggtaagat ttaaaggctt
     3121 agtaagagga aggtcgtaac caatttgcaa gttcttggct tggaagataa agcgccctgg
     3181 tgtacgagct ggtttgaaat caaaggatgg ttttggtttc tcactttgga gttcgataat
     3241 atccatctta tccaatttct tttgacgaga catagccata ttacgagttg caacacgggc
     3301 tttattacga gccacaaagt ccttgaggtc tgcaatctct ttctgctggc gttcgtaggc
     3361 tgcctctagc tgagatttct tcatagcata aacttcttgg aactggtagt agtcaccaga
     3421 gtaacgcgtc agctgttgat tttccacatg atagacaata ttgataacgt cattgaggaa
     3481 tggaatatcg tgcgaaatga gaacaaaggc attctcatag ttttggagat agcgcttgag
     3541 ccaatcaata tgctcagcat ccaagtagtt ggtcggctcg tccaacagca agatatcagg
     3601 cttttcaagg agaagttttg ccaaaagcac cttggttctt tgcccacctg acaaagaagt
     3661 tacatccgta tccatgccaa agtccataac accaagagca cgcgctactt cgtcaatctt
     3721 agcatccaag gtatagaaat cacgactctc cagacggtct tgaagttctc ctacttcttc
     3781 catgagagca tcaacatccg caccgtcttc agccatttcc atatagaggt cattgatacg
     3841 agcttcagct ttgaaaagct catcaaaagc cgtacggaga acatcacgca ccgactgtct
     3901 ttcagcaagg acagagtgct gatccaagta accagccgtc acatatttgg accactcaac
     3961 ctttccttca tctggcagca ttttaccagt cacgatactc ataaaggttg attttccttc
     4021 accattggca ccgaccaggc cgatatgttc tcccttgagg agacggaagg acacatcttc
     4081 aaaaattgca cggtcaccaa aaccgtgact cagattttta acttctaaaa tactcatttt
     4141 aattccttat cttgttttta tgtaatcgtt tataaaggag ccaagccaga tagccaccca
     4201 aagtgttggt ccacaaatca tcaatctcaa agacgcgatt gaaatcaaag aaaaagtcca
     4261 agattaattg cgtacactcg attccaagac tcacaagaaa actaaaaaga aggacctttt
     4321 ttgttttccg caaatttgga aatagataaa ggagttggaa aatcagagga aaaaacaaga
     4381 agacattgag gatattttgt aaaaaaatcc aacataattg tccaatgtca ctcacttcgc
     4441 ccagtttcca gagagaattg aaaggagtca aaagaaaaac caggcgtcca agatgctgaa
     4501 tatctggagt tcccactccc acggtagatt gttcttgagg agtaaagcaa aaatagacaa
     4561 tgcaaatgct atagaaaatg actccccaga ccaaaatatg attataagtc ttcttcatca
     4621 ttaaggattt accgctgcga ctgccttctg gcggtcacgt ttcattgtgt taaagagcaa
     4681 ttgtccacaa gctgcgtcaa tatctgtacc atgctcttga cgaaccacac agttgccccc
     4741 ttttttctta agcgtatcat agaaagccaa cacgcgctct ttgggactac ggctatattg
     4801 gtcatgctca ctaactgggt tataaggaat caagtttaca taagacgatt tcttgatgtt
     4861 cttgagcaat tcagccaatt ccaaggcttg ttccacaccg tcgttgactt cattaagcat
     4921 gatatattca aaggttacac gacggtttgt tgtctcaatg tagtattcaa tagcagcaaa
     4981 gagtttt
//
LOCUS       DAVBHS010000134         4919 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVBHS010000134 DAVBHS010000000
VERSION     DAVBHS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.134"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            387..1748
                     /gene="trpE"
                     /locus_tag="VQ651_001744"
     CDS             387..1748
                     /gene="trpE"
                     /locus_tag="VQ651_001744"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET2754701.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQAIDRDPLDFLYEVTHKSQHHSDLPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRSVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            1745..2311
                     /locus_tag="VQ651_001745"
     CDS             1745..2311
                     /locus_tag="VQ651_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET2754702.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            2322..3326
                     /gene="trpD"
                     /locus_tag="VQ651_001746"
     CDS             2322..3326
                     /gene="trpD"
                     /locus_tag="VQ651_001746"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET2754703.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEDYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            3323..4090
                     /gene="trpC"
                     /locus_tag="VQ651_001747"
     CDS             3323..4090
                     /gene="trpC"
                     /locus_tag="VQ651_001747"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET2754704.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDC
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNTGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            4077..4676
                     /locus_tag="VQ651_001748"
     CDS             4077..4676
                     /locus_tag="VQ651_001748"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET2754705.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            4654..>4919
                     /locus_tag="VQ651_001749"
     CDS             4654..>4919
                     /locus_tag="VQ651_001749"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET2754706.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGA"
BASE COUNT         1444 a          951 c         1225 g         1299 t
ORIGIN      
        1 ggtgtaccga gatgaacatc tcttcatcaa tgtctctggt cgtggaggca aggacctagc
       61 tgcgattgca gattactaga agctaaaaaa taatagatgg aaaaattaca gtcttttctc
      121 tcacagagag cttgtggttg ctgaaaacaa gcagagaaag ctgtaaagtt gggttcatct
      181 gaaacgagag aagaaaacat cattctcaag ggagtgccct ttatcgcaca gcctgttaca
      241 ggagatatct gagacaggaa tgagagatag gagaaatcct ataattgagg tggcaccgcg
      301 aatttcgtcc tcacgcaagt tattttgcgt ggggattttt catacagtcg ccacgaacta
      361 gaattagaac tgaaagggaa atgacaatgg aacgaatcat tcatggagat gtcttatcac
      421 caatcttggc ttatatgcgc ttaaaggggc aacataaggt tattttagag agtattccga
      481 gagacaagga aacagctcgt ttttctatcc tagcctataa tccagttttt gagattaagt
      541 ttgaaaatgg agttctctat caaaatggtc aagcgattga tcgggatccc ttggatttcc
      601 tttatgaagt gactcataag agccagcacc attcagacct cccttttggt gggggagcca
      661 ttggttttgt tggttacgat atgatttcgc tttatgaaga aattggtcaa atccctgagg
      721 atacaattgg gacgccagac atgcatttct ttgtctatga gagctatatg gtctttgacc
      781 acaagaagga gaagatacat gtcatagagg atgcacttta tagtgagcgc agtcaagagg
      841 ccttggaaaa atccttgaac caagtgcttg aggaattacg cattcctgct ccaaatgaat
      901 ttgaagactt ggatttatca ccacttgact tcaaaccgca tatcgctcca cataagtttg
      961 agggaatggt agaaacagct cgtgacttga ttcgtaacgg agatatgttc caatgtgtac
     1021 tcagccagcg tttctcagca gaagttactg gaaatccatt tgacttctac agaaatctcc
     1081 gcgtgactaa tccatctaat tacctttatt tctatgattt tggggattat caaatcatcg
     1141 gagctagtcc agaaagtttg gtttctgtca aaaatggcat cgtgacaacc aatccgattg
     1201 cagggacgcg accaagaggg gctacggatg aagaagacaa ggccttggcg actgacctcc
     1261 tctctgatga gaaggaaaca gcagaacatc gaatgttggt agacttgggg cgtaacgata
     1321 ttggccgcat ctctgaaacg gccagtgtcc aagttactaa gtatatggag gtggagctct
     1381 tccgctacgt catgcatttg accagtgtgg tcaagggacg tttgcttgca gaactcactg
     1441 ccatggatgc cttgaaagca acgcttcctg ctggaaccgt ttctggagca ccaaaaattc
     1501 gagctatgag acgcatctat gaactggaaa cggaaaaacg gagcgtatat gcaggagcaa
     1561 tcggctactt gtctgcgacg ggtgatatgg atttggccat tgccatccga accatgattc
     1621 tcaaaaatca aagagcctat gtgcaggctg gggcagggat tgtctacgac tctatcgccc
     1681 aaaacgaata ccaagaaacc attaacaaag caaaatctat gactagaatt ggagaactaa
     1741 gaccatgatt ttattgattg acaactatga ttcttttacc tataacttgg cccaatacat
     1801 tgggaatttt gcagaagttc aggttttgag aaatgatgat tccaagctgt atgaagaagc
     1861 tgaaaaagca gatggtctgg tcttttctcc tggtcctggt tggccagttg atgctggaaa
     1921 gatggaagac atgatttgtg attttgcagg caagaagccg attctaggga tttgtttggg
     1981 tcaccaagcc attgcagaag tctttggtgg taagctaggt ttggctccaa aagtcatgca
     2041 tgggaaacag agcaatatca actttgaagc gccatctgtt ttgtatcaag gtattgagga
     2101 tggacgtgcg gtcatgcgtt atcacagtat tttgattgag gaaatgccag aagactttga
     2161 agtgacagct cgttcgactg atgaccaagc catcatgggg attcaacata aaaacctacc
     2221 gatttatggc ttccagtacc atccagagag cattggaacg ccagacggct tgtcttctat
     2281 tcggaatttt atcgagaagg ttgtaaagtg aggaaactag gatgaaagag attattgaaa
     2341 aactagcaaa atttgaaaat ttatcaggtg tggaaatgac ggatgtcatt gagcgtatcg
     2401 taactgggcg tgtaactgaa gcgcagattg cttctctcct cttagctctt aagatgaagg
     2461 gggaaacacc tgaagaacgt acagccattg cccaagtcat gagaggacat gcccagcata
     2521 ttccaactga gattcatgat gctatggaca actgtggtac aggtggggac aagtctttca
     2581 gttttaatat ttccacaact gcagcctttg tcttggctgg tggcggtatt cacatggcca
     2641 agcatggtaa ccgctcgatt tcttctaaat ctggttccgc agatgtcctc gaagccttgg
     2701 gaatcaatct tgacctcaaa ccagctgaac taggtaaggt ctttgataaa actggaatcg
     2761 tctttctctt cgctaaaaat atgcacccag ctatgaaata catcatgcca gctcgtttgg
     2821 aattgggaat tccaacgatt atgaacttga ctggtccact gattcatcca atggctttag
     2881 aaacacagct tcttggaatt agtcgtccag aactcctaga aagtacagct caggttttga
     2941 aaaatatggg tcgcaaacgt gccatcgtgg ttgctggacc agaagggttg gatgaagctg
     3001 gcttgaacgg aacaaccaag attgcacttc ttgaaaatgg cgaaatcagc ttgtcaagct
     3061 ttactccaga ggatttggga atggaagact atgctatgga agatattcgt ggtgggaatg
     3121 ctcaggaaaa tgcagaaatt ttgcttagcg ttctgaaaaa cgaagcaagt ccattcttgg
     3181 aaacgacagt cttgaatgct ggtcttggtt tctatgctaa tggtaagatt gatagcatca
     3241 aggaaggagt tgccttggcc cgtcaagtga ttgctagagg caaggccctt gaaaaactca
     3301 gactgttaca ggagtaccaa aaatgagtca ggaattttta gcacgaatct tagagcagaa
     3361 ggcgcgtgag gtggagcaga tgaagctgga gcaaatccag cctctgcgcc agacctatcg
     3421 cttggcagaa tttttgaaga atcatcagga ctgcttgcag gtaatcgctg aggtcaagaa
     3481 agctagccct agtttgggag atatcaatct cgatgtggat attgtgcaac aggcccagac
     3541 ttatgaagaa aacggagcag tgatgatttc ggtgttgaca gatgaggttt tctttaaagg
     3601 gcatttggat tatctacggg aaatttccag tcaggtagag attccgacgc tcaacaaaga
     3661 ctttatcata gatgaaaagc aaatcatccg cgctcgcaat acaggtgcga cagttatctt
     3721 gcttattgtg gcagccttgt ccgaagaacg cctcaaggaa ctgtatgact acgcgacaga
     3781 gcttggtctg gaagtcttag tggagactca caatctagct gaactagagg tagcccacag
     3841 acttggtgct gagattatcg gggtcaacaa ccgcaacttg actacctttg aggtcgactt
     3901 gcagaccagt gtagatttag ccccttactt tgaggaaggt cgctattaca tttctgaatc
     3961 tgccattttc acagggcagg atgcggaacg actagcccca tactttaacg gaattttggt
     4021 agggacagct cttatgcagg cagaaaatgt ggcccagaga atcaaggagt tgcagattga
     4081 caaaggttaa aatttgtgga ctatcgacca aagaagcggt ggaaacagcc gtttcagcag
     4141 gagccgacta tatcggtttt gtctttgcac ctagtaaaag acaggtgact ttagaagagg
     4201 cagctgagtt ggcaaagctt attcctgcag atgtgaaaaa ggttggagta tttgtttcac
     4261 caagtcgggt agaactgctg gaagcgattg acaaagttgg cttggacttg gttcaagttc
     4321 acggtcaggt ggcagctgat ttatttgaga atttgccttg tgccagtatt caggctgtgc
     4381 aggtagatgg aaatgggcat gtccccaatt ctcaggcaga ttatctactc tttgatgccc
     4441 ctgtggcagg aagtggtcag tcctttgatt ggggtcaact ggatacgact ggactagcac
     4501 agcccttctt tatcgcaggt ggccttaatg aagataatgt agtaaaagca attcaacatt
     4561 ttactcccta tgcagtagat gtatcgagcg gagtggagac agatggacaa aaagatcatg
     4621 aaaagattag aagatttata gagagggtaa agcatggcat atcaggaacc aaataaagat
     4681 ggattttacg gaaaattcgg cggacgtttt gtcccagaaa cattgatgac agcagttttg
     4741 gagttggaga aggcctaccg tgaaagtcag gcagacccaa gtttccaaga ggaattaaac
     4801 cagcttttgc gccagtacgt aggacgtgaa actcctcttt attacgcaaa aaacttgacc
     4861 cagcatatcg gcggagccaa gatttatctc aaacgggaag accttaacca tacaggagc
//
LOCUS       DAVBHS010000135         4838 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVBHS010000135 DAVBHS010000000
VERSION     DAVBHS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.135"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(193..1608)
                     /locus_tag="VQ651_001750"
     CDS             complement(193..1608)
                     /locus_tag="VQ651_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET2754707.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSAIPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1621..3459)
                     /locus_tag="VQ651_001751"
     CDS             complement(1621..3459)
                     /locus_tag="VQ651_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2754708.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPDVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTPAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     NLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3477..4316)
                     /locus_tag="VQ651_001752"
     CDS             complement(3477..4316)
                     /locus_tag="VQ651_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HET2754709.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            complement(4616..4798)
                     /locus_tag="VQ651_001753"
     CDS             complement(4616..4798)
                     /locus_tag="VQ651_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET2754710.1"
                     /translation="MKSQKIKVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTIL
                     RLESASIVSELVAGLR"
BASE COUNT         1628 a          936 c          626 g         1648 t
ORIGIN      
        1 attcagtcta ctatatataa ccaatctatc ctaagttaac tatttataat cggctctctg
       61 taacaaaatt aaattaatta atccccctat aaaataaaga ggtttagaat ttgatgtctt
      121 ccaaacctct ttattttata tttaatgaga tgttgcctta accgtgataa tatctaatca
      181 tcaataaaca aactatttga ataaggattc tccatttgat tcaatcactc ctttatacca
      241 agtaaaggac attttcttat atcgattgaa tgtgccattt ccatcatcat ttcgatcaac
      301 ataaatgaga ccgtaccttt tagaaagttg tgcagtggac atagaaacac agtcaataca
      361 tccccaagac gtatagccca taatttcaac accatcctgt agagcttcag caacttgcaa
      421 taaatgttct ttcatatact gaattctata atcatcttgg acggttaagt tattaagttc
      481 atcttttatt agttgatctt tagcacctaa tccattttct actataaata atgggatttg
      541 ataacggtca taatatctat ttaaaattat acgtagtcca attggatcaa tttgccatcc
      601 ccactctgaa gactctaaat aaggatttac taaaccacca ataatattcc cttctcctga
      661 attatactgt gttggaatag cagattgagt cacactcatg taatagctaa aggataaaaa
      721 atctacagta taatttttta ataactctgc atcttcagct gcaaactcta tgttaatgtc
      781 attttcctta aaatatcttt ttgcataatt cggataataa cctctaacat gcacatctga
      841 aaatagataa tttagattct catactcatg agtcgcccat acatcttttg gatttggagt
      901 cattggataa gctggcatag ctaataccat acaccccacc ttaaactctg aattaatctc
      961 acgagcaatt tttgtaacca aactcgaggc gactaattca tgatgtatag cttgatataa
     1021 ttcttgtttc gaaagattct ccttaggtat atctattcct ccactagtaa acggtaattc
     1081 caaaacagag tttacttcgt taaatgtaag ccaatattta actttatctt tatacctttc
     1141 taaaactgtt cgagcaaatt tttcataaaa atgaatcatt ctcctatcaa tccatccatg
     1201 atattttctt gctaaatata atggagtctc atagtgtgaa agagttacaa gtggttctat
     1261 cccgtgagca tgtagttcat caaataattc atcataatat ttcaaaccag cttcgttagg
     1321 ttcttcctca tctccttttg gaaaaattct actccatgca atagaagtac gaaaaacatt
     1381 aaagcccatt tcagaaaaca aggatatatc ttccttatat ttatgataaa aatcaatacc
     1441 tatcaatttt aagttatctt ctgtaggatt ttctgttgct tctcctaatc cacctttggg
     1501 taacacatcc tgaactgata agcccttacc atcttcatta tatgctccct ctacttgatt
     1561 agctgcaacc gctccacccc aaagaaaatc atctggaaaa atggtcataa ctttcctcca
     1621 ttataatatt accagtaatt ccttagaatg ctcgattgtc tgattattag gtaatactaa
     1681 tacatctaga aaatcattgg tattcgttac aattactggt gtaactgttt cgtagccttt
     1741 agtcttgatt aaattcaagt tcatttcaaa aatcaactga tttttgaaaa ctctgtctcc
     1801 ttcttctaca tgactaataa aaccttgacc ttttagctca acagtatcta atccaatatg
     1861 aattagtaac tcaacaccct catcactctt caatccaatt gcgtgcttag tcggaaaaat
     1921 atttgtaatt ttcccatcaa atggtgcata aaccttacct tcacttggga taatcgctac
     1981 tccgtctcca attaatttat ctgaaaatgt tttatcctgg acatcgctta acggaatgat
     2041 ttctcctgat ataggagaaa atatcatttt tttatttgaa actccagctt taacttctaa
     2101 attgctagaa ctctcttctt catcgattcc aaatatataa gctaatacaa aggtaataac
     2161 aaccgaaatg accgccacaa ttaaagcatt tacaatattt gatggcacat cagaataaat
     2221 aaattgaggc aacgctatca aagatgggac agcaaataga tatgctttaa cactagtaag
     2281 acctgcaaat aatcccgcta atccaccacc aatcatagct gcataaagcg gttttttata
     2341 ttttaaagtc acaccatata atgcaggttc ggtaatccct gcaagtaagg ctgagaaacc
     2401 tgctgcaaaa gcaatttgtt ttgtattatt atttttactc tttaatgcaa cagccatcga
     2461 agcagcccct tgagctaagt ttgaccctaa cattgctgga agaattaata cgtctggagt
     2521 agcaatagat gccgccaaaa aaataggtgc aaaagcccaa tgcattccag tcataacaat
     2581 aaatggcata atagcaccaa gaatagctaa tgtaagccat ccagctacac catacatttg
     2641 cccaactaga tttgataatc cttcaccaac aattactcca ataggtccga ctacaactaa
     2701 ggcaatacag cttgatacta ataatactag cgtaggttgc aaaaaactct tagtaatagc
     2761 tggtgttaat ttagcaatta ttttttcaat atatttcatc aaccaaacca taataagaat
     2821 tggaacgact gatgaaccat aactagctgg tgtcacaggt gcaccaaata aactaagagg
     2881 attccctgat tgcaccattt gaacaaaatt tggatggaga agtacacctg ctacagacat
     2941 agctaatgta gatgttactt ttaatttttg tgatgcagaa taagctaata acagcggtaa
     3001 gaaataatat ggagcatccc caaaaaatgt caaaaaagca atagtctgag aatctgattg
     3061 caatatacca agcattggta aaatgattac caagactttc aacatacctc cccctaacat
     3121 tgctggaatg attggagtca tggaaccagc gatatactca atgattcttt ctaaaatatt
     3181 ccctttgtgc ccttgaacaa ctgaatcgga ttcaaaattg ccaagtttaa cgaattcttt
     3241 ataataatta gctacatcat taccaagtat aatttgatat tgtccattct ttttcataat
     3301 acctattaca tctggtatct tcttcacatc atcatcattg actaaatttt catcttttaa
     3361 ttctaatctt aaacgtgtta cacaatgggt aactctattg acattttttt cacctccaat
     3421 tacatcgagg attttttgta ccgtatcttt ataactcatg gtattctcct attctattaa
     3481 tctaaatttt ttgttaagcg acgaatatga gccatcaaat aaactaattc actagaagtc
     3541 agcaaataat tgtactcagt ttgtataaac attgccacct gttcaccaca ttcatattct
     3601 ctaggatatt tatttttcat taatgctaac aagtcttcat catcatcgtc acaataagtt
     3661 gtattttcaa ccaagcgatg agcaaataat tttatatgag ttatgaatct ataataatca
     3721 atagaatctt ggttaaactc agtacaatag aaatcttgaa tgattttctc aattcccata
     3781 attatttcag tgattttata cgactcttga aaattatttt ctaaattagc attaacaaaa
     3841 tgtagcgcta tgaatgcagc ttcgtcaatt ggaagttcaa tacctaactc tttttttatt
     3901 atttgtaaag cccttttccc aagttcaaat tcttctttat aatatttcga tatttcccag
     3961 cgtaggggat tcgaaataat aatcccttct ttataacgtt ctatgctcga agaaatatga
     4021 tccgttaaat taatatagat aatttcatca aaattattac ccaatgttat tttacctagg
     4081 ttaattattt tttcggtgat gtacaccaac tccaaaggaa cgttaataaa catctctgta
     4141 aaacgggtca tattatctct atttttgaga aaaaaacttt tctcaatatc aattggattt
     4201 actttatctc cagtttttct tccaaaagct atgccccttc caaaaagaag aatatcgata
     4261 tcttttttac tttgcgcaat tacggcattg tggtttagta ttcgttttat gatcataaaa
     4321 tttccctttt aaaataaaag gaacctactt aaaaacatca agaaacttct tccgatgtga
     4381 ttaaataggt tcctgctcac ttgtattagt gataacatcc ttttatttca atttttaatg
     4441 gtgacatgaa gcttaaataa ttaataactc tgtaaccatt ttcattttat gatatttcaa
     4501 tctatttgtc aagtacttgt agaaattttt ttattttgtg tgattcgaat gtgataatgg
     4561 caacagtctc actaaagaaa tgaaaatcat acatagaaat ttttaaaaat tttctctatc
     4621 gtaaacctgc cactagttca gaaactatac tagctgattc caaacgtaat atggtcatct
     4681 catctacctt ccctaataat ggcattgcac cataccaaac aatagtctta ttcaccaaaa
     4741 gaataggcgt tactattttt gattgagtaa aagacacttt aattttctgg ctcttcaact
     4801 gtgaactaca ccccaaaagt tagacagaaa aaatctaa
//
LOCUS       DAVBHS010000136         4825 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVBHS010000136 DAVBHS010000000
VERSION     DAVBHS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.136"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(7..1221)
                     /locus_tag="VQ651_001754"
     CDS             complement(7..1221)
                     /locus_tag="VQ651_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2754711.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1377..1670)
                     /locus_tag="VQ651_001755"
     CDS             complement(1377..1670)
                     /locus_tag="VQ651_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754712.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVKVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            1832..2284
                     /locus_tag="VQ651_001756"
     CDS             1832..2284
                     /locus_tag="VQ651_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET2754713.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(2319..3707)
                     /locus_tag="VQ651_001757"
     CDS             complement(2319..3707)
                     /locus_tag="VQ651_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2754714.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            3773..4735
                     /locus_tag="VQ651_001758"
     CDS             3773..4735
                     /locus_tag="VQ651_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET2754715.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
BASE COUNT         1409 a         1129 c          954 g         1333 t
ORIGIN      
        1 caagccctat ctacgatact gtttcaagaa acgagtcatc ttttcttgga tttgggctaa
       61 ctcatcaaca cgaggaaggt aaacgatacg gaagtggtct ggttcctgcc agttaaagcc
      121 tcgaccatga accaagagaa ccttttcctg cttcaagaaa tcaaggacaa actgctcatc
      181 atcatcgata cggtacatat tgcggtcgat ttttgggaag atatagagcc ccgccttggg
      241 tttaacggca gacaaacctg gaatatcttg aatggcatta tagatgaaat ttctttgctc
      301 gtagattcgt ccaccaggaa gaagcaattc atcgactgat tggtgacccc ccaaggaagt
      361 ttgtacgact tgttgggcca aaacgttaga gcaaaggcgc atattggaca gcatattgag
      421 cccttcgata tagcccttaa catgagtctt agggccagac aagaccatcc aacccacacg
      481 gaaacctgct atgcggtggg attttgacag accattcatg ctgacacaga agacatctgg
      541 tgccaagctc gccacaggcg tatgcacatg tccgtccatt accatgcggt catagatttc
      601 atccgcaaag ataatcaaat cgttttgacg ggcaatctca ataatctcca gcaagagttc
      661 cttaggataa agggctccag ttgggttatt tggattgata aggacaattg ccttggtatt
      721 ggaagtgatt tttgacttga tatcgtcaat atctgggtac cattccacag cttcatcaca
      781 gatatagtga acggcatttc ctccagctag gctgacagcc gccgtccata gaggatagtc
      841 tggcattggc actaagacct catccccatt gtccaaaagc ccctgcatgg acatgacaat
      901 caactcactg acaccatttc caaggtaaat atcatcaata tctacgtttg ggaatttctt
      961 cagctggcaa tactgcatga tggccttacg ggctgagaaa atccctttgg agtcagaata
     1021 gccttcacta tcacgcgcat tcataatcaa gtcatgaatg acctcgtctg gcgctgtaaa
     1081 gccaaattct gctggatttc ctgtattcag acgtaaaatc ttttctccat tcgctcgcat
     1141 ccgcatggct tcttccaata caggaccacg gatatcataa gcaacatgct ctaacttact
     1201 agacttgtta tattctttca tcttgtttcc tcaattcatc aggatagtaa cattatacca
     1261 ctagaaagag aatgcgacaa gtttactgaa atgtgttact agtttcgtat agaagaaaaa
     1321 ccttgctaaa ggcgaggtta agaacgtaga gatagcaggc attttgcaaa gacacatcac
     1381 ataccaaaag ataaacctag aaaaagcaag cctagaaagg agtcataaag aagattaaac
     1441 atgaggaaaa gcccccatat catcaagtaa tcccaacgcc cacttcgttt tgcaactaga
     1501 aataagatta gatggtagag tacatgaaag actaaaaatc caataaaact tggataaaga
     1561 agcggtacca gactctctaa ttgacagatc atgatcactt ttaccaagac tgaaactagg
     1621 ataattccat aatacaggaa atttgatttt tgaaaagagt ttttcatcat tctcccttcc
     1681 ttcacttctt tccttctatc ctttctttta ttttatcata tatttatata gattttaagc
     1741 cctaatattc ttttgaaaaa tacttttttt gctttacttc tatagtgaaa aggtttacaa
     1801 ttatagtaag aaaatttcag gaggtttcat tatggcacaa cgttaccaaa atatcatggt
     1861 cgcaatcgat ggttctaagg aagcggactt ggcttttgtc aagggagttc attctgctct
     1921 acgaaacgac gctaaactca ccatcgcaca tgtcattgac acacgcgctc tccaaagcgt
     1981 atccaccttt gatgctgaag tttacgaaga actccaagtc gacgctgaaa gtctgatgaa
     2041 agagtacgaa aaacgtgcta aagatgctgg ggtagcagat gttcatatcg tcattgaaat
     2101 gggaaatcca aagaccctgc tagcacgtac tattccagat gccgaggaag tggacctcat
     2161 cctcgttggc gcaactggtc tcaacgcctt tgaacgcctc ttggtcggct cttcatctga
     2221 atacatactc cgccatgcta aggtcgattt gctggttgtg agagaacaag aaaaaacctt
     2281 ataatcacaa agaaaaggag cccctagctc ctttttgttt acgatttatt tctctcttta
     2341 tggcgttcgt aagccttgag ctggcgctgc agttcctttt taatagcagg ttctggagca
     2401 tatttttctt cccaattatc tggttttaag attttatggg tcactggatc aaaatgagcc
     2461 ttgccatctg gaaaaatttt ccccatattg gcctgatgga caatatcaaa aatacgttct
     2521 gggtccaccc ccatcaagac aaaactgccg taggtgaagt aaagcgtgtc aatcaaggca
     2581 tccacttgcc ctatcaaatc ttgctgagca ggtgtcttct tggctacttt acctgctgcc
     2641 ttatcaaggg cctgatgaag ttgcgataca gcttgaccaa aatcttcttc agaaggactg
     2701 gctgctcgaa caaactccac caattcttct attttaaaac cagccctatg ggttgcaccc
     2761 tctaaatccc aagctcgagg ttcttcttgg gttcgttcat ccatcatgtg gtggaaggtc
     2821 ttgaccttat tgaaatgata gtcacggctg acaaagactt tttctgaaga caaaacacca
     2881 aaatgttcca aggccttgtg gatcccatct tgttgattgc tagtggtaat gtgcttggca
     2941 gcttccttga cactgctact accatttccc atagcgaccg acataccgac gccagccagc
     3001 atttcaaggt cattatctga gtcaccaaaa gccatgactt ggttgaggtc aaagccatat
     3061 tctttcccaa ctcggcgaat gccttctaat ttagaatttc cctgattgat gacatccgat
     3121 gcaaaaggat tgctacgtgt caatttcaag tcttcaaaat cagctgccgc cttctcagat
     3181 tcttctggtg tcatcagcat caaaacttgg tagataggct gattcatcag gtgaagcagg
     3241 tcctcttcct tttggggaac aaccttgctg accatgcgat taaaagactg actcaccgtc
     3301 cgagttaaaa cagagggaac gaagcgacta attcgttggg aaaaagaacc cagaccaaag
     3361 gacatgattt tagaacccaa catggcatcc ttggtcccta gggcaatctc cgtgccctct
     3421 tttttagcat agctaattag atggcgcaaa tgtaacttgg aaatagggct cgtgaacaag
     3481 actctgtctt tactaaagat atactggcca ttataggtta ccgcaaaatc cagatccaaa
     3541 tcgtccatca attccttaac aaaaaaaggt cctcgccctg tcgctacgcc aactagtacc
     3601 ccttgttctt tgacaatctt aatcgcgtcc ttagtggatt tcaaaacact cttgcgattg
     3661 ttgaccaagg ttccatcgat atcaaaaaaa acagctttga cttccatcct attccaatct
     3721 ccccttttgt gatacaatga ttataccaca tttcagaaag agtgagtaaa tcatgcctaa
     3781 gaaaatcctt gttttacata cgggtggaac tatttccatg caggccgatg cttctggcgc
     3841 tgttgtgacg agttcagata atcccatgaa ccatgtgtcc aacccacttg aaggaatcca
     3901 agtccacgcc ttggactttt ttaaccttcc aagtccccat atcaaaccca aacatatgct
     3961 ggtcctctac cagaaaatta aagaggaagc agataactac gatggagtgg taatcacaca
     4021 cggaaccgat actttagagg aaacagccta tttccttgat accatggaag ttccccatat
     4081 gcctatcgtt ctaacaggag ccatgcgtag ctccaatgag ctcggtagtg atggtgttta
     4141 taattaccta agtgctttac gagtggccag cgatgacagg gctgctgaca aaggagtttt
     4201 ggtcgttatg aacgatgaaa tccacgctgc caagtatgtc accaaaacac atacgactaa
     4261 tgtcagcacc ttccagactc caacacatgg ccccctcggt ctcatcatga aacaggaaat
     4321 cctctacttc aaaacagctg aacctcgtgt tcgctttgac cttgatcaca tacaaggttt
     4381 agtccctatc atctcggctt atgctggtat gacagatgag ctgattgata tgctggattt
     4441 agaacacttg gacggtttga ttatccaagc cttcggagct ggtaatattc ccaaagaaac
     4501 ggctcaaaaa ttagaaagcc ttctgcaaaa aggaatccca gtcgctctgg tatcacgatg
     4561 ctttaacggt attgccgagc ctgtttatgc ctaccagggt gggggcgtac agttgcaaaa
     4621 agcaggcgtt ttctttgtta aagaactcaa cgcccaaaaa gctcgcttga aactcctcat
     4681 cgccctcaat gccggactaa caggacaggc tttgaaagac tatatggaag gctaatactc
     4741 ttcgaaaatc tctgcaaacc acgtcagagt cgccctaccg tatgtatggt tactgacttc
     4801 gtcagttcta tctacaacct caaaa
//
LOCUS       DAVBHS010000137         4642 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVBHS010000137 DAVBHS010000000
VERSION     DAVBHS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.137"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(91..1839)
                     /locus_tag="VQ651_001759"
     CDS             complement(91..1839)
                     /locus_tag="VQ651_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754716.1"
                     /translation="MKRQTVNQTLKRLVVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKELSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTISRVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            complement(1841..3565)
                     /locus_tag="VQ651_001760"
     CDS             complement(1841..3565)
                     /locus_tag="VQ651_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754717.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFLSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENLPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            complement(3623..4561)
                     /gene="msrB"
                     /locus_tag="VQ651_001761"
     CDS             complement(3623..4561)
                     /gene="msrB"
                     /locus_tag="VQ651_001761"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET2754718.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
BASE COUNT         1385 a         1021 c          970 g         1266 t
ORIGIN      
        1 tattacagta aacaattttt ctagctattg taacaaaaaa gtcactcgaa ggcaactttt
       61 ttcgtccata aactttcaaa agagaatggc ttattcagaa ctaaaagccg cagctttttg
      121 catttggtaa tacttaccct ttctatccat gagttcttga tggttaccat attcaacaat
      181 atcaccatct actaagacaa gaattaaatc cgcatcctga atggttgaca aacggtgagc
      241 aatgatgaag cttgtgcggc ccttcatgag ttttgcaaag gcatcctgta ccagcacttc
      301 tgtccgtgta tcaatggaag aagttgcctc gtctaagata agaatctttg gaatagccag
      361 aaagactcgg gatatggtca agagctgagc ttggccgaca gagagagatt ctccagcatt
      421 ttccaacttg gtatcgtatc cctgtggcaa ctgttggatg aaaaagtctg cattagctgc
      481 tttggcagca gcaattactt gctctcgact ggcttcagga ttgccaaagg caatattatc
      541 atgaatggtc ccttgtgtga gccaggtttc ttgaagcacc ataccaaact gctgtctcaa
      601 tgatactcgt gtataatcat aaatggattg cccatccagc aagatatctc ccgagctaat
      661 gggataaaaa cgcataagga gattgataag agttgatttt ccagcacctg tcggaccaac
      721 gatggctacc ttactaccag ctggaatatc gatagacaac tccttaatca aaattttttc
      781 aggatggtag ccaaaagaga catgtttaaa ggaaatagct cccttaactt ggtcactggt
      841 caagacttcc ttacctgttt cagccacttc agggctatct aagaccccat agatacgctc
      901 tacgcaagcc agagcacttt gcaactcagc tagcactgaa gaaatatcgt taaagggctt
      961 ggtgtattgc tgaacatagt tcaaaaaagt cactaaacga ccgacggtca aggttgaacc
     1021 catcatgata cgataagctc ctactccagc taaaagggca taaatgagtg catttacaaa
     1081 gcgagtcgaa ggattgaccg ttgaagaata aaagatggct gactgagaat agcctgagta
     1141 gttgtcatga gcctcacgca atctttggat aaattctgtt tgagcattga aggactggat
     1201 tatagtctgc tggctaagcg attcttcaat caactgagtc tgaattcccc tcgtctctgt
     1261 ttgcttctgg aagagatgat aggatctctt ggcaataaag cgtgaaatca ccatggacag
     1321 tggcgtcaac agcaagacta agagagtcat gaggagatga atttggagca tggctagaat
     1381 actaaccaaa atcatcaaaa caccaatgaa aaattggtta aaaatcatgg tcaagccagc
     1441 tgccaactgt tcgatatccg tggttacacg actgaccatc tctccattgc cttgccgatc
     1501 cgcaaaagca atcggtaacc gatggagttt atggatgatt cgctctcgta aatctctggt
     1561 ataagagaag attagacgat tatagaggag aggattggcc cattgtacca gagtatttcc
     1621 tattaccacc aagagcatct ggagaaaaat ctgccaaaaa actggtgatg aaccagccac
     1681 taggacttgg tcaatgacct gcccaatcag aataggtagg taaattgata agccaacttg
     1741 ggcaatagtt cctagaaagg ctaggaaaag gaggaaggga tggtttgcta aatctacgac
     1801 taaacgtttg agcgtctggt ttacagtttg tcgtttcatt ctagtcctcc tttccatgtt
     1861 gggatgcatt gatttcacga tagacttgac tggatttcat caagtcatcg tgcttgccaa
     1921 cagctagcaa ctcacctttt tccaagagga gaatctggtc cgccatctgg agtgtcgaag
     1981 tccgttgaga aatcaagatt aagctcatat ttggcagatt ttcgcggata gctttcaaga
     2041 gcttggactc tgtaatggtg tccagtgctg aggtcgcatc atctaggatg agaaacggag
     2101 cctggcgcaa gactgctcgt gcgatagaca gcctttgttt ttgtccacct gagaaatttc
     2161 gccctcctgc ttcaactaga gcatccaaga gtccttcctt atcactgaca aaatccttag
     2221 cttgcgcaat ctccaaggct tgccagagtt cttgatcagt cacttcttga tttaaaccta
     2281 aagtcaagtt ggaacgaata gttcccttaa agagttcgac cttttgaggt acataggcaa
     2341 tccaagaccg ccactgctcc aaattaagag gactacgtcc attttgataa aggtcaatgt
     2401 tccccttgtc tactggataa agtccaagta agagttgcac caagcttgat ttaccagaac
     2461 cagtcccccc aatgatacca aggatttgtc cttgagtcat atcaaaggaa atgtctctca
     2521 gagaaggctg ggccgcatca ggataggtaa aagtcaattc ttggacttgt aaaacccgac
     2581 cactggtaac ttgcttttgt tctaattctg aatggatgtt ttcgggagat tccgcaaaga
     2641 cctcctcgat tctcttggct gagatatagg actggttgag ggaattgatc agcatggcta
     2701 gcttgaccaa ttccaccaaa atctgtaaga ggtaattgat aagagcaatg agagcacctt
     2761 gactgagtac tcctccttga attgaaatat agccttgcca gataataacg agaagagttc
     2821 cattgacaat cagataggtc agaggtgtta ataaactaga caagaaacct gtcttttctt
     2881 gtaatctagc ataaacttgg ttaagggttt gaaaaatctg taactctcgt ttttcttgac
     2941 caaaagcacg aataacccgc atcccttgca attgctggcg cgtttcctga accagttggt
     3001 ccgttttctt tctgagacta ctgtagagag gattgaccaa tcgagataac cctacaatga
     3061 caatggtcaa aatggcaacc atgactaaga accagaaagt caactcagct gagattcgat
     3121 aagccataaa aatggcacca aaaacgataa tgggcgctcg taaaaagaga cgcaggaatt
     3181 gattgatacc agtctgaatc tggtaggtat ccgaagtcaa gcgagtaacc aaactagaag
     3241 ttgtcagacg gtctctgctg tcctttggca aggaaagaat atgacgataa agatcgtttg
     3301 tcaattcctt agcaaaacct actgctgcct ttgctgagta aaattgagct atcaaggcca
     3361 ctaaaacgcc aattactgca aagataagga gcaggccaat ctgcatccag agatgacctt
     3421 gatctccctg aggtaaagat tggtcaacaa tcccagcaat caccatggga accaagagct
     3481 caaaaacagc ttctaacagc ttgaacaagg gggctaaaat tgattccttg atgtagggtt
     3541 tgaagtaaga taataggtgt ttcataaatc ccttctattc atattctaga aatgaagaaa
     3601 gtgggaagcc ccactctctg ttttatttgt ttaagtaagg caatagatag ccatatcctg
     3661 ctttttccat ctcatccttg gccacaaagc gtaaagaagc agaattgata cagtaacgga
     3721 ggccgcctaa ctcccgcggt ccatctgtga aaacatgacc caagtgagca ctgcctgaac
     3781 gagaacgaac ttcaattcgc tccattccat ggctcagatc cttgtaataa tgaatcaact
     3841 ctttggaaat cggacggcta aaacttggcc aaccacaacc tgaagcaaac ttatccttgg
     3901 caaaaaagag tggctcacct gtcgtaatat ctacataaat cccctcttca aaggtttggt
     3961 cataggcatt ggtaaatgga gcctctgtag cagcttcttg tgtgacacga taagactctt
     4021 cagatagact ggccttcaac acctcttgac taggcttttc atagtttgct gcatcaatca
     4081 atggcttatc agcatcggtc acatcgatat gacagtaacc tgaaggattc ttcctgagat
     4141 agtcttggtg gtagtcttca gccagaatgt agtggcgtaa ttgctccact tctactgcaa
     4201 tctttcgacc cagcatgcgt tcctgctcct gcaccactgt gtagatagct ggcaaatctg
     4261 cttcatcctg ataataaatc ccagttcgat attggcgacc acggtcattc ccttgttgat
     4321 tgatagatag aggatcgata actcggaaat aataaagtaa aatctctctg agtgacactt
     4381 ccttctcatc gtaaatcact tggaccgttt ctgcatggtc tgtttccttg atcaactggt
     4441 aattggtcgt ttcgacttga ccattagcgt agccaacact ggtttctagc actccagaaa
     4501 tgcgtgaaaa atattcctct aggccccaaa aacaaccacc tgctagataa atttctgcca
     4561 tctttatttc tcctttatca cgtttttaac taatactctt cgaaaatctc ttcaaaccac
     4621 gtcagcgtca ccttaccgta gg
//
LOCUS       DAVBHS010000138         4579 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVBHS010000138 DAVBHS010000000
VERSION     DAVBHS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.138"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            376..1311
                     /locus_tag="VQ651_001762"
     CDS             376..1311
                     /locus_tag="VQ651_001762"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HET2754719.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            1345..2382
                     /locus_tag="VQ651_001763"
     CDS             1345..2382
                     /locus_tag="VQ651_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET2754720.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNSSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            2555..3160
                     /locus_tag="VQ651_001764"
     CDS             2555..3160
                     /locus_tag="VQ651_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET2754721.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            3316..3846
                     /locus_tag="VQ651_001765"
     CDS             3316..3846
                     /locus_tag="VQ651_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HET2754722.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            3867..>4579
                     /gene="rlmN"
                     /locus_tag="VQ651_001766"
     CDS             3867..>4579
                     /gene="rlmN"
                     /locus_tag="VQ651_001766"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET2754723.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQGIVQESADGTVKYLFELPDGMLIETVLMCQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSISGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFP"
BASE COUNT         1418 a          846 c          984 g         1331 t
ORIGIN      
        1 tcctgttctt gtttcatttt actatatttc tttaaaaatg ataaaggttg agatttctcc
       61 tcgtaataga taatcatggg gatatttcaa tccaaagttt tattcgttat cacttgacta
      121 ttgcaaggtt ttctagagca acagagtcat ggaatggact catggttgag atttctcctt
      181 gttgcttgga cttcattcaa aagtctgtta cccaagcctt gttcaaactt ccaatacact
      241 agctgtttcc atagcatgac ttctgtacta gactttcttt tccgaataaa tagatagaac
      301 cacagaatct agtaaaccta gaattaaaat tatggtataa tattagcaat aaaagaaatc
      361 tggaggatta gaatcatggt atcaacgaaa acacaaattg ctggttttga gtttgacaat
      421 tgcttgatga atgcagcagg tgtggcttgt atgacgatag aggagttaga agaggtcaaa
      481 aactcagcgg caggaacctt tgttactaag acagcgacct tggacttccg tcaggggaat
      541 cctgagccac gctaccaaga tgttccactt ggttccatca actctatggg cttgccaaat
      601 aatggcttag actattattt ggattatctt ttagatttgc aggaaaaaga gtcgaaccga
      661 actttcttct tatctctggt cggcatgtct ccagaggaaa cccatactat tttgaaaaaa
      721 gtccaagaga gtgattttcg tggtctgact gagctaaatc tttcctgtcc aaatgttcca
      781 ggtaaacctc agattgccta tgattttgag acaacagacc ggattttggc agaagtgttt
      841 gcttacttca ccaaacctct tggaattaaa ttgccacctt attttgatat tgttcacttt
      901 gaccaagcgg cagctatttt caacaaatat ccgctcaagt ttgtcaactg tgttaactct
      961 atcggaaacg gcctctatat agaagacgaa tctgtcgtta ttcggcctaa gaatggtttt
     1021 ggtggaattg gtggagaata catcaaaccg actgctctag ccaatgttca cgccttttat
     1081 caacgtttaa atcctcaaat ccaaattatc ggaacaggtg gcgttctgac tggtcgagat
     1141 gcctttgaac acatcctctg tggagcaagt atggtgcagg tgggaacgac ccttcacaaa
     1201 gaaggcgtca gtgcttttga ccgcattacc aatgaactga aagcaatcat ggtggaaaaa
     1261 ggctacgaga gcttagaaga tttccgtggg aaattgcgct atattgacta aattaaatcg
     1321 aaaaatctga agaaaggaga gacgatgcta gccattgaag aaagtcagaa gttgacttta
     1381 tcaaatttac cgagcctgag cctatttaca gggacagatc agggtcagtt tgaagtgatg
     1441 aagagtcaag tgttgaaaca gattgggtat gattctgctg acctcaactt tgcctacttt
     1501 gatatgaaag aagtagttta caaggatgtg gaactggagt tggtcagcct tcctttcttt
     1561 gcggatgaga aaatcgtgat attagattat tttatggata tcacgactgc taagaaacgc
     1621 tttttgacag atgatgagct taagtcattt gaggaatacc ttgacaattc ttctccaaca
     1681 accaagttga taatctttgc agaaggaaag ctggatagca aaagacggtt agtcaaatta
     1741 cttaagcgtg atgccaaggt cttcgatgca gtagaagtaa aagaacaaga attgcgccag
     1801 tatttccaaa agtggagtca gaaacaaggt ctgcagttta ccaatcattc ttttgaaaat
     1861 ctcctcatca agtcggggtt tcaatttagc gaaatccaga aaaatcttct ctttttacag
     1921 tcctataagg cgaattctgt tattgaggaa gaggatattg ttaacgcaat tcccaagacc
     1981 ttgcaggaca atatttttga tttaactcag tttattctga ctaaaaagat ggatcaggcg
     2041 cgcgatttgg tgagagactt gaccttgcaa ggggaagatg aaatcaaact gattgcagtc
     2101 atgctgggac aatttcggac ttttactcag gtgaagattt tggcggagtc tggccaaaca
     2161 gaatcgcaga ttgcaagtag tttaggtagt tatctgggac gtaacccaaa tccttatcaa
     2221 atcaagtttg cattaagaga ttcgagagga ctttctttga gctttctgaa gcaagctatt
     2281 tcctatttga ttgagacaga ctatcagatt aagacaggtc tttatgaaaa aggtttcctt
     2341 tttgaaaagg cactcttaca gattgctagt caggtcaatt gacatttgtt gaaactacaa
     2401 cccgcgtcag gattatcttg tcgtgggttt cttgctgatt tgcttatttt attagaaagt
     2461 atgtcagtga tttccttatc tataagcaaa aaacttgaaa aaagtgaatg tttgcgttat
     2521 cattttcata tagaaagaaa aagaggtatt acagatggct attatcttac cagaacttcc
     2581 atatgcatac gacgctttgg aaccatacat cgatgcggaa acaatgcatt tgcaccatga
     2641 caaacaccat caaacttatg tcaacaatgc caatgcagct ttagaaaaac accctgaaat
     2701 cggtgaagac cttgaagcct tgcttgctga tgtagaatct atcccagctg atatccgtca
     2761 agcacttatc aacaatggtg gcggacactt gaaccacgct cttttctggg aattgatgac
     2821 tcccgagaaa acagctcctt cagcagaact ggcagcagca atcgatgcaa catttggttc
     2881 atttgaagaa ttccaagcag ccttcactgc agcagcaaca actcgttttg gttcaggttg
     2941 ggcatggttg gttgtcaaca aagaagggaa acttgaagtg acttcaacag caaaccaaga
     3001 cacaccaatc tcagaaggta aaaaaccaat cttgggcttg gacgtttggg aacatgctta
     3061 ctacgtgaaa taccgcaacg tgcgtcctga ctacatcaaa gctttctttt cagtaatcaa
     3121 ctggaataaa gtagatgaat tgtacgcagc tgctaaataa tgatagttgg agggaagaat
     3181 tgttcttctc tttttaggtt ataagattct ggtctgacaa aatcgtcaga cttttttcat
     3241 ttttatgaga aacgtgctaa ctgctagaaa ttatggtaga ataaagtatt aagtaatcgt
     3301 ggaaaaagga tatctatgcg aaaagaaatt gcacctgaat tatacaacta taacaagttt
     3361 cctggtccgg agttccattt acacggggat aaggtcgaaa cggaagggat agctttttcc
     3421 ttggttgaaa atatcaagga tgcctttgat gtgacgactt ttaatcagcg tttttcagaa
     3481 gtcttaacca agtttgatta tatcgtgggg gactggagca acgaacagct tcgcctacga
     3541 ggtttttaca aggatgaccg aacagaagaa aaacttgaaa aaatcagtcg tttacaagac
     3601 taccttttag agtattgtag ttatggttgt gcctattttg tcttagaaaa tgaagcccct
     3661 aagcgagcat catttgacaa gaaaatgcgt aagacggaag aagaaacacc ttctagaaaa
     3721 ggaaagaaac cgactcaaac taaacgaaag tcgaatgcag ataagaaaaa tagacgtcgt
     3781 cagaaagacc agcattctca gaaagaggac aaggaacaac gtcattttgt cattcgtcag
     3841 aagtgagtag agaataagga gaagaaatga aaccctcaat tcatagtttg gcacatcaaa
     3901 ccatgcagga atgggtattg gagcagggag aaaagaaatt ccgtgcagat caaatctggg
     3961 aatggctcta ccgtaaacgt gtgcagtcat ttgaagaaat gaccaacctt tccaaggatt
     4021 tgattgctaa gctcaatgac cagtttgtgg tcaatccctt gaaacaaggt attgtgcaag
     4081 agtctgctga tggtacagtc aaatatctct ttgaattgcc tgacggtatg ttaattgaga
     4141 ctgtactcat gtgtcaacac tatggtttat ctgtctgtgt gaccactcag gtcggctgta
     4201 atatcggttg taccttctgt gcctctggtt tgattaagaa gcagcgtgac ctcaataacg
     4261 gtgaaatcgt agcgcaaatc atgctggttc agaaatactt tgatgagcgt ggtcaggatg
     4321 aacgcatcag ccatatcgtt gtcatgggaa tcggtgagcc ctttgataat tacaacaatg
     4381 tcttgaattt ctttcgtact atcaatgatg ataaaggaat ggctatcggt gctcgtcaca
     4441 tcacggtttc aatctcaggt ttggctcata aaattcgtga ctttgctgat gaaggggttc
     4501 aggtcaatct tgccgtgtct cttcacgcac ccaacaatga acttcgttca agtatcatga
     4561 agattaaccg tgcctttcc
//
LOCUS       DAVBHS010000139         4556 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVBHS010000139 DAVBHS010000000
VERSION     DAVBHS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.139"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            63..227
                     /locus_tag="VQ651_001767"
     CDS             63..227
                     /locus_tag="VQ651_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HET2754724.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            306..1034
                     /gene="yaaA"
                     /locus_tag="VQ651_001768"
     CDS             306..1034
                     /gene="yaaA"
                     /locus_tag="VQ651_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HET2754725.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(1085..2569)
                     /locus_tag="VQ651_001769"
     CDS             complement(1085..2569)
                     /locus_tag="VQ651_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2754726.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEGYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYISGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            2785..>4556
                     /locus_tag="VQ651_001770"
     CDS             2785..>4556
                     /locus_tag="VQ651_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2754727.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAVSVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTGEV
                     TKPAEKVEEAKDKKEEVTHQDAIDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADASEAELSAAESSIQSSIIGLELRSNSNKGTVSETPVAKKANIVEAKEETN
                     PAATTDRSAVDSAVLPTSTAAKVETTSAPASINEILKPGLSLSDARQNPAIRKEDVDR
                     GYSGFRAAPAPTNQPVTNLGNNTVFTDISRGSHTFRGNGNSRRGNPIHFDVTTTRVRN
                     RVDFSIRYSGPGEFVNNNFILDKGNGFGEPSRATVSTPKLRDQVKNISKGGNFVSHSG
                     YSLEAAVETSTNQTIKFSLPIANPNGDLSVRLRPVTFNVGQGESGSATSNDPYSNSNY
                     YFREKPLLLDANPNGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEKGQRQIIY
                     KVHQLGNETILGLPISNSVIKEVKPRIMQIGVAKDLIDTVKPRVDQNKVGDTNNLTFY
                     LDNDGNGVYTEGVDELVQKIAIKDG"
BASE COUNT         1552 a          749 c          910 g         1345 t
ORIGIN      
        1 aaggtttatt taataaatat atgttataat aaggtaaatt aagcctaatg gaggacaaag
       61 aaatgggttt ttatttgatg gttgcttcaa tgctactcgg actgctcgct ttgaagatag
      121 gtttttctca gttcaaggag aaaaaagata aattcttatc tatcttaaca agtttagctg
      181 gcacagccct tgttctcgtg gctgtctggt taggatggcc caaataaata gaaaaacctc
      241 ggtcaaaccg aggtttttca aatgaatttc ttggttgtgg ctaaaaaata tgctacacta
      301 tcaatatgaa aattttaatc ccaacagcaa aagaaatgaa cacagacttg ccaagtatcg
      361 aggcaattcc tttaaaacca gaaagtcaga ccgtgcttga tgccttggct ctctattctg
      421 ccagtcaatt ggagagtttc tacaaggtat cagctgagaa agcggcggaa gaatttcaaa
      481 atatccaagc tttgaaaagg caaactgctc aacactatcc agccttgaaa ctttttgatg
      541 ggcttatgta ccgcaacatt aagagagata agctgaccga ggcggaacaa gattatcttg
      601 aaaatcatgt cttcattacc tcggctttgt acggtgttgt tccagccttg tcacccatgg
      661 ctcctcaccg tttggatttt ttgatgaaat taaaagtcgc tggtaagact ttgaagagcc
      721 attggaaggc agtctatgat gaagctctga agaaggaaga agtgattttc tctctcttgt
      781 catcagagtt tgagactgta ttttctaagg aaatcagagc aaagatggtg accttcaaat
      841 tcatggagga tagaggcggt cagctgaaga ttcactcaac tatctccaag aaagcgcgcg
      901 gggcctttct aacagcttta atagaaaatc aagtacaaac tgtgggggaa gcacgtcgct
      961 tgaactttgc tggatttgtt taccgagaag atttgtcaca accacaggga ttggtttttg
     1021 taaaggaagt atagaaatca gataagagaa aagtcagcac gattatgctg acttttttga
     1081 taacttataa agtattggac aaatgaatct catttgataa ggtgttaaga tgattaacca
     1141 aattcatgat tgacagatga tttcggcaaa taaacttctt ggtaatccct gaaatataga
     1201 aattagacac gatgatatcg taatctgtct gatttaaaat ctcaggactt gtttttaatt
     1261 catcccaaat atcgaaggta aagcggttat tacagtaata ggaaagcata tcaacaaagg
     1321 taagggatat agcatggtca aagttactga taatcaaaac cttgataggt ggtcgatttt
     1381 ctaacaactg agtagagagg ttttcagcat gggttaagat ggtgtacatc aagtgttcca
     1441 actgttcagg atggtcatgc tgtccaattg cctgacggta ttgagccagt tcttgacgcg
     1501 cacttcccat aaagtctggg aagtaacctt caaatttttt aatcgtcaat gctttttgtt
     1561 caaataagat tggggtagaa aagatttcct gtctttcaaa gaaagcagtg ttgtgtaaat
     1621 gccaaatcag ctcatcgtgg ttagtaaaag taatcttata ttgttttgat aggcgttcta
     1681 atatttgact aagtaattgg taagagtatc ttgcttgact gttatcttct aaagagttga
     1741 agaattcttg aggatctagg aaaatatcat tctgaagaaa agaaataaaa atttgagcga
     1801 ttgtatctgg agtcatttca agcccaaaat gtttagagaa ataagcaagt gtctcctgga
     1861 aatttggaat gctctttaaa agaggataat attctttgta aaagtggttg gggaggtcaa
     1921 tgaagtgacc atttttaacc ctgtggatac tgatagctat catcagtttg tacattctat
     1981 aaattgaaaa tcgcattgga taattcgtga ttttatagaa gaagtcagca aactctgtta
     2041 aatcttcctc aggaaggtca ggaaaaggcc aatctaggaa ataatagcgc tcagaaaagt
     2101 attgggcaaa aaagtaacga atatcgatct catttccacg tatctcggat ggagtaaaag
     2161 ataattcaat ttgaaactga gatgaaagaa cctttgtaat atttctgccc agtcgataga
     2221 ggttggcact actaaagtaa agattttcta ttgttcgata aataggtagc ccctcattga
     2281 agaacatata ctctagtaat tgaaaagatt gagaattagc aagaaaatat tgataaatat
     2341 cttctacact agtgttaact tctaaatcta tcatgattcc gttaacagaa gattggatgt
     2401 taatagatgg aaaggcaata cgtaattcat ttaaatcact ttttaagatt cgttctgtac
     2461 atcctaattt ttcggctagt tcatgtaatt tcatccaatt acgattctga attagggttt
     2521 ccattaagcg taattgtctt tgaacttttg tggataaaag gtttctcata cgggtatttc
     2581 ctccataatt cattatatct ttttttgtct aagtagtata aaaaaattag agcaagaaaa
     2641 ttgtcgttag taatcggaaa aattagaata atataagtgg cttttaagtg caaaaacaga
     2701 atatattaat tgttcagaaa atattgatta atgcctcaaa tagttgtaca ttgtaaacta
     2761 taaaatacta tctaggagtt taaaatgaaa cattcacata aaaaatcatt tgactggtat
     2821 agcatgcaac aacgttattc tattcgtaag tattactttg gtgcagttag tgtcttgctc
     2881 ggtaccgctt tggtattagg tgcagcagct agtgtccaaa cagtacaagc ggaagaaaac
     2941 aaacaagaaa ctaccaatag tatttctgtt ggtaggggag aagcagctac taaaccagca
     3001 gaggtttctg cgtctaataa agagaaaacc tatgcagctc caactgtagc taatccagta
     3061 gaaacgactc cagttaaaac tggagaagtt actaaaccag cagaaaaagt tgaagaagca
     3121 aaagacaaaa aagaggaagt aacgcatcaa gatgctattg acaagtcaaa attgttaacg
     3181 gctctttcgc gtgctaaaaa gttagaaagc aagttatata cagaagcaag tgctgctaac
     3241 ttgcaaacaa gtatccaagc tggtcaaagc ttgcttggga aagcagatgc atctgaagct
     3301 gaattatcag cagcagagtc atctattcaa tcatctatta ttggtctaga acttcgttct
     3361 aactctaaca aaggaactgt atcagaaact cctgtagcga agaaagctaa tatagttgaa
     3421 gcgaaggaag agactaatcc agctgcaaca actgatcgtt cagctgttga tagtgctgtt
     3481 ttgccaacta gcacagctgc caaagtagaa acaacttcag ctccagcatc tattaatgaa
     3541 atcttgaaac caggtttgag cctttctgat gctcgccaaa atccagctat ccgtaaggaa
     3601 gatgttgata gagggtatag tggttttaga gcggcgcctg ctccaacgaa ccaaccagta
     3661 actaatctag gaaataatac tgttttcact gatattagca gaggttctca tactttccgt
     3721 ggtaatggaa attcacgtcg tggaaacccg attcattttg atgtaacaac aactagagtt
     3781 agaaatagag tagatttctc aatcagatat tcaggaccag gtgagtttgt taataacaac
     3841 tttatcttag acaaaggaaa tggattcgga gaaccttctc gtgcaactgt ttcaacacca
     3901 aaattaagag accaagttaa aaacattagt aagggtggaa actttgtttc tcattcaggc
     3961 tacagtttag aagctgcagt agaaacaagc accaatcaaa caattaagtt tagtttacct
     4021 atagccaacc ctaatggtga tctgtctgtt cgattaagac ctgttacttt taatgtaggt
     4081 caaggggaat caggatctgc aactagtaat gatccatata gtaactctaa ctattacttc
     4141 agagaaaaac cactcctctt ggatgcaaat ccaaatggtg gtactaataa taagactgtt
     4201 tcagaagaca ttgacttcca aactgtctat cttccaacta gtaagttacc agaaggtcaa
     4261 actagattag ttcgagaagg tgaaaaagga caacgtcaaa tcatctataa agttcatcaa
     4321 ttgggtaacg aaacaatcct aggattgcca attagtaatt cagttattaa agaagttaag
     4381 ccacgtatta tgcaaattgg tgtggctaaa gatctaatcg atacagtaaa accacgtgtt
     4441 gatcaaaata aagtcggtga tacaaataac ctcactttct atcttgataa cgatggaaac
     4501 ggtgtttata ctgagggcgt agacgaactt gttcaaaaaa ttgctattaa agatgg
//
LOCUS       DAVBHS010000140         4466 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVBHS010000140 DAVBHS010000000
VERSION     DAVBHS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.140"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            157..1416
                     /locus_tag="VQ651_001771"
     CDS             157..1416
                     /locus_tag="VQ651_001771"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET2754728.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            1432..1983
                     /locus_tag="VQ651_001772"
     CDS             1432..1983
                     /locus_tag="VQ651_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2754729.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            1976..3253
                     /locus_tag="VQ651_001773"
     CDS             1976..3253
                     /locus_tag="VQ651_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2754730.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIDRLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            3269..4129
                     /locus_tag="VQ651_001774"
     CDS             3269..4129
                     /locus_tag="VQ651_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HET2754731.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
BASE COUNT         1336 a          718 c         1028 g         1384 t
ORIGIN      
        1 tctttgattt tcattgagta ttagtaattc agttactaac tcgtcaactc tgatttatcc
       61 aataaaattg aaaaggatgg aaaaaaggat aaatttatga tatactttat tttgaagacc
      121 ttattagaaa tcttgaaaga gtattgaaaa cttagaatga gaaaaattgt tatcaatggt
      181 ggattaccac tgcaaggtga aatcactatt agtggtgcta aaaatagtgt cgttgcctta
      241 attccagcta ttatcttggc tgatgatgtg gtgactttgg attgcgttcc agatatttcg
      301 gatgtagcca gtcttgtcga aatcatggaa ttgatgggag ctactgttaa gcgttatgac
      361 gatgtattgg agattgaccc aagaggtgtt caaaatattc caatgcctta tggtaaaatt
      421 aacagtcttc gtgcatctta ctatttttat gggagcctct taggccgttt tggtgaagcg
      481 acagttggtc taccgggagg atgtgatctt ggtcctcgtc cgattgactt acaccttaag
      541 gcgtttgaag ctatgggtgc cactgctagc tacgagggag ataacatgaa gttatctgct
      601 aaagatacag gacttcatgg tgcaagtatt tacatggata cggttagtgt gggagcaacg
      661 attaatacga tgattgctgc ggttaaagca aatggtcgta ctattattga aaatgcagcc
      721 cgtgaacctg agattattga tgtagctact ctcttgaata atatgggtgc ccatatccgt
      781 ggggcaggaa ctaatatcat cattattgat ggtgttgaaa gattacatgg gacacgtcat
      841 caggtgattc cagaccgtat tgaagctgga acatatatat ctttagctgc tgcagttggt
      901 aaaggaattc gtataaataa tgttctttac gaacacctgg aagggtttat tgctaagttg
      961 gaagaaatgg gagtgagaat gactgtatct gaggacagca tttttgtcga ggaacagtct
     1021 aatttgaaag caatcaatat taagacagct ccttacccag gctttgcaac tgatttgcaa
     1081 caaccgctta cccctctttt actaagagcg aatggtcgtg gtacaattgt cgatacgatt
     1141 tacgaaaaac gtgtaaatca tgtttttgaa ctagcaaaga tggatgcgga tatttcgaca
     1201 acaaatggtc atattttgta cacgggtgga cgtgatttac gtggggccag tgttaaagcg
     1261 accgacttaa gagctggggc tgcactagtc attgctgggc ttatggctga aggtaaaact
     1321 gaaattacca atatcgagtt tatcttacgt ggttattctg atattatcga aaaattacgt
     1381 aatttaggag cggatattag acttgttgag gattaaaccg tagaggtgtt tatgaatatt
     1441 tggaccaaat tagcaatgtt ttcttttttt gaaacggatc gcttgtattt gcgtcctttc
     1501 ttttttagtg atagtcagga cttccgcgag atagcttcaa atccagaaaa tcttcaattt
     1561 attttcccaa cgcaggcaag tctggaagaa agtcaatatg cactggccaa ttactttatg
     1621 aagtcccctt tgggagtgtg ggcaatttgt gaccagaaaa atcaacaaat gattggttct
     1681 attaaatttg agaagttaga tgaaatcaaa aaagaagctg agcttggcta ttttttgaga
     1741 aaagatgctt ggtcgcaagg atttatgaca gaggttgtta gaaaaatttg tcagctttct
     1801 tttgaggaat ttggcttaaa acaattattt atcattaccc accttgaaaa tgaagctagc
     1861 caaagagttg ctcttaagtc tggatttagt ttgttccgtc agtttaaggg aagtgatcgt
     1921 tacacaagaa aaatgcggga ttatcttgaa tttcggtatg taaaaggaga gttcaatgag
     1981 taagcatcag gaaattctaa gctatttgga ggaattacca gtaggtaaaa gggtcagtgt
     2041 tcgtagcatt tcgaatcatc taggagttag tgatggaaca gcctatcgag ctattaaaga
     2101 agctgaaaac cgtggaattg tggagacccg tcctagaagt ggaacaattc gtgttaaatc
     2161 ccagaaagtt gctatagata gattaacgtt tgctgaaatt gcagaagtga cttcttctga
     2221 ggttctggct gggcaagaag gtttagagag agaatttagt aagttttcaa ttggtgccat
     2281 gactgaacaa aatatcttgt cttaccttca tgatgggggg ctcttgattg tcggagaccg
     2341 aacccgtatt cagttgctag ccttggaaaa tgaaaatgca gttctggtta cagggggatt
     2401 tcaggttcat gatgatgtgc ttaaactggc caatcaaaaa gggattcctg ttctaagaag
     2461 taagcatgat acctttaccg tcgctaccat gatcaataaa gccttgtcaa atgtccaaat
     2521 caagactgat attctgacag ttgagaaact ttatcgccct agtcatgagt atggttttct
     2581 gagagagaca gatacagtta aagattattt ggacttggtt cgtaagaatc gtagcagccg
     2641 tttccctgtt atcaatcaac atcaggtcgt tgttggcgtt gtaaccatga gagacgctgg
     2701 tgataaatca ccaagcacga caattgataa ggttatgtct cgtagtctat ttttggttgg
     2761 attatcgaca aatattgcca atgtgagtca acggatgatc gcagaagact ttgaaatggt
     2821 accagttgtt cgaagcaatc aaactttgct tggcgttgtg acgcgacgag atgtcatgga
     2881 gaagatgagc cgttcccaag tttcggctct accaactttt tctgagcaga ttggacaaaa
     2941 gctctcttat caccatgatg aagtagtcat tacagtggaa ccctttatgc tagaaaaaaa
     3001 tggagttttg gctaatggtg tattggcaga aattctgacc cacatgaccc aagatttagt
     3061 tgttaatagt ggtcgcaatc tcattatcga gcagatgctg atctactttt tgcaggccgt
     3121 tcagatagat gatatattgc gcattcaggc acggattatt catcatacga gacggtcagc
     3181 tataattgat tacgatattt atcatggtca ccagattgtt tcaaaagcaa atgtgactgt
     3241 taaaattaat tagaaactag gagaaaagat gataacatta aaatcagctc gtgaaatcga
     3301 agctatggac aaggctggtg attttctagc aagtattcat ataggcttac gtgatttgat
     3361 taagccaggc gtagatatgt gggaagttga agaatatgtc cgccgtcgtt gtaaagaaga
     3421 aaatttcctt ccacttcaga ttggggttga cggtgccatg atggactatc cttatgctac
     3481 ctgttgctct cttaacgatg aagtggctca cgctttccct cgtcattata tcttgaaaga
     3541 tggtgatttg ctcaaagttg atatggtttt gggaggtccc attgctaaat ctgacctaaa
     3601 tgtctcaaaa ttaaacttca acaatgttga acaaatgaaa aaatacactc agagttattc
     3661 tggtggttta gcagactcat gttgggctta tgctgttggt acaccgtccg aagaagtcaa
     3721 aaacttgatg gatgtaacca aagaagctat gtacaagggt attgagcaag ctgttgttgg
     3781 aaatcgtatc ggtgatatcg gtgcggctat tcaagaatac gctgaaagtc gtggttacgg
     3841 tgtagtgcgt gatttggttg gtcatggtgt tggcccaact atgcacgaag aaccaatggt
     3901 tcctaactat ggtattgcag gtcgtggact ccgtcttcgt gaaggaatgg tcttaaccat
     3961 tgaaccaatg atcaatacag gcgattggga aattgataca gatatgaaaa ctggttgggc
     4021 gcataagacc attgacggtg gattgtcatg tcagtatgaa caccaatttg tcattacgaa
     4081 agatggacct gttatcttga ctagccaagg tgaagaagga acttattaat aaaaagtgaa
     4141 aagactactg gaagtttatt atgaaaaaat ccagtagatc ttttcataat aaaacgcatt
     4201 gtatcaagtg ttaggggctg atatcatgcg tttttctgct tttaagattt tttccaactc
     4261 tgtttgtaag cgcatcataa caaagggtct aggattcagg gctctcctcc tatatactat
     4321 tagtaaagta aaactaaggg aggatatttt agtgtcgcag tctattgttc ctgtagagat
     4381 tccacaatat tgtcgttttg attctaaaaa gagaaatgga attctgttta atgttcgtat
     4441 tgccaatctt aaatttacct ttttta
//
LOCUS       DAVBHS010000141         4438 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVBHS010000141 DAVBHS010000000
VERSION     DAVBHS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.141"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..119
                     /locus_tag="VQ651_001775"
     CDS             <1..119
                     /locus_tag="VQ651_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2754732.1"
                     /translation="QQGFTDRLVGYSSSIALVFTTLIGMISLVQRRVLKEDD"
     gene            121..966
                     /locus_tag="VQ651_001776"
     CDS             121..966
                     /locus_tag="VQ651_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2754733.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFSGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRLAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
     gene            976..2295
                     /locus_tag="VQ651_001777"
     CDS             976..2295
                     /locus_tag="VQ651_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET2754734.1"
                     /translation="MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(2503..2575)
                     /locus_tag="VQ651_001778"
     tRNA            complement(2503..2575)
                     /locus_tag="VQ651_001778"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2540..2542),aa:Lys,seq:ctt)
     gene            complement(2839..4110)
                     /locus_tag="VQ651_001779"
     CDS             complement(2839..4110)
                     /locus_tag="VQ651_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET2754735.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWIKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
BASE COUNT         1294 a          809 c          976 g         1359 t
ORIGIN      
        1 atcaacaagg ttttactgat cgtcttgtag gatattctag ctcaatcgct ttagtattta
       61 caacgttaat tggtatgatt agcttagtac aacgtcgcgt tctgaaggag gatgactaag
      121 atgaaacgat ttaaaccaac aacaatctta gaatatattc tgttaacttt attggcagga
      181 ctcttccttt ttcctatggt ttggatggta gtttcagcta tgaagccaga agcggatgtt
      241 tataaaaatt tgactagttg gcaagccttc ttaccaagtt taaatcccgc tgattggttt
      301 aaaccttatc aggaagtatt tgagcgcttt agtattttta catatctagg gaacagcatt
      361 ttttatgctg gaacgtttgc tattggttct attattgtca atgcactggc cggtttcgct
      421 tttgcaaaag tcaatttttc aggtaagaaa attttatttg gtttcttact tgctttactt
      481 attattccag tggaaactgt gttaattcca caatttacca ttatcaattc cttaggtttg
      541 gtcaataatc gtcttgcagt tattattcca ggattggcga gtgtatttaa tatttactta
      601 tttaggaatt tctttattgc aattccagaa gagattcttg agtctgcaaa gatggacgga
      661 gcaagtatcg ttagaatttt ctttagaatt atgcttccaa tgtctaaacc tgcagttgct
      721 acagttggtg ttttatcatt tatttcaagc tggaatgatt atatctggcc attgatggtt
      781 ttgacagatt cttctaaatt ttcaatgcag gttgcaatta caactattaa tacaactcaa
      841 ccagtttata tcaatcaggt aatggctgta ttaactattt caacgattcc gttgattttg
      901 gtctatgtag tcgctcagaa atatattgtt caaggtctgg gtggttccgg aactggaata
      961 aaataggata tacttatgat aaataaaaca tttacagtag aaaaagccaa tcgttttata
     1021 gcagaaaata aacatctcgt taatactcaa tataagcctg aagaacattt ttcagctgag
     1081 attggttgga tcaatgatcc aaatggattt gtctattttc gtggagaata ccatctcttt
     1141 tatcaattct atccatatga tagtgtttgg gggcctatgc actggggaca tgctaaaagt
     1201 aaggacttgg tgacttggga gcacttgcca gtggcacttg ctcctgacca agattatgac
     1261 cgaaatggtt gtttctcagg ctctgccatt gtcaaggatg atcgcctctg gctcatgtac
     1321 actggacata tcgaagaaga aaccggtgtc cgccaagtgc aaaatatggc attttcagat
     1381 gacgggattc actttgaaaa gatttcccaa aatccagttg caactggatc agacttgcca
     1441 gatgagttga ttgctgctga tttccgtgat ccaaaactct ttgaaaaaga tggacgctat
     1501 tactccgtag tagctgccaa acacaaggat aatgtgggct gtatcgttct actagggtcc
     1561 gataacctag tagaatggca gttcgaatcc atctttttaa aaggaggaga acaccaaggc
     1621 tttatgtggg aatgcccaga ttacttcgag ttagatggga aagattgcct tattatgtca
     1681 cccatgcgtt atcagcgtga gggagactca tatcataaca tcaactcatc gcttttgttc
     1741 acgggtaagg tagattggag agaaaaacgt tttatcccag aatcagttca agaaattgat
     1801 catggccaag acttctatgc gcctcaaaca ttgttggacg atcaaaatcg tcgtatcctg
     1861 attgcttgga tgcagacatg ggggcgtacc cttccaaccc atgaccaaga acacaaatgg
     1921 gcatgtgcca tgactttacc tagaattcta agattggaag atggcaaact aagacaattc
     1981 cctgttaaaa aaggccaata tcaaatccaa atagataaag attgtcatta ccacttagga
     2041 aatgatacag attatcttga atttggttat gacagtaatg cgcagcaagt ttacattgat
     2101 cgtagccatc ttattcaaaa aattctaggt gaagaagaac aggacactag tcgacggtat
     2161 gtagatattg aagctaaaga attggaagtt gttctagata aaaattccat cgagattttt
     2221 gtcaatcaag gtgaagcaag cttgactgca acttattact taacggtgcc agctgagcta
     2281 tcacgaattg attaaaaatt aagttatttc tcctaaagaa aaagttctct ttctaaaata
     2341 gtggaaagag gactttttgt gttttgggta tataagctta gtttatggta tttgtaaaat
     2401 tggtgttgga ttatgattta agctagtttt ctaaagaatt tgaaaaaaat tttatttaag
     2461 caaaaaaacc ttggttccaa ggcttttcct gttgtattta gatgccccct acagggatcg
     2521 aacctgtgac ccacggatta agagtccgct gctctgccag ctgagctaag gaggcaaaga
     2581 aaaagctgta ttggtgccga aacttcacgg tttgtattga acccgcgcaa ttaagcaggt
     2641 gggcaactcg ctctaactga agctgtttcc gtgtgagacg gcctacatgc tgttagaaga
     2701 cttttgtttc cctaataata caaaaaatag tcggtcaaca cttaagtgtg aagtcgtaca
     2761 ccacagcgtt tctatactta tatgatacca ctttttcaaa aaaaatcaag aggaaagtgc
     2821 aattttttga aaaggatttc agatttttcg caaacgatcg atagcttcct ttctttgagc
     2881 caaagcccgt tcatatttac cagtatcttc tgcttggaaa tagtgatgat tacgaatttt
     2941 ttctggcaga tagtcttgct tgatccaatt tccaggatag ttgtgtggat agagatagtc
     3001 ttgggcattc cccagttcct tgcttccact gtagtgccca tcacgcaggt gtcgcggaat
     3061 aggcaagtgc cctgatgttt tgaggtcagc aagtgcctta tccatagcta cataggctga
     3121 gttggatttt ggagaaaggg ccaaatcaat cacgacattg gcaatgagaa tgcgggcttc
     3181 ggggaaacca atcctttgtg cagcatccag agcagtcacg gtatgaatct gggcctcagg
     3241 attggctaag ccaatatctt cataggcgat aacagtcaag cgacgagcga gactaggcag
     3301 atccccagcc tcaatcaagc gggcagcata gtggagactg gcatccacat ctgagccacg
     3361 gatagacttt tgcagggcag atagaacatc atagtgaccg tctccatcct tgtccatagt
     3421 gatgtagctt ctctgaagac tattttccat gatgtctaag gtgatgtggc gaatgccctc
     3481 gtcattctca ggggtagaga gaacagccaa gtccagtgag ttaaaggcag agcgaaggtc
     3541 tccgtttgta gaggttgcga tgaaatccag cgcatcctca tctagttcta ttggaaaatc
     3601 aaaaccacgt tcagggttac ttagagctat ctgcagggcc tctttgacgt cttggttaga
     3661 cagaggttcc aactcgaaaa tttgaacacg gctacgaatg gcaggagtga cagagaagaa
     3721 aggattttca gtcgttgctc caatcatgat gaccagtcca ctttccaaga gaggcaagag
     3781 gaagtcttgc ttggtcttat ctagtcgatg aatttcgtct agcaatagga cgagaccacc
     3841 agaaaattta gcttcttccg agatttcttg cagtcgcttt ttactatcaa ctgtcgcatt
     3901 gaaagttcga aaggcatact tggtcgttcc agcgatggca gaggcaatac tggttttgcc
     3961 gattccagga gggccatata gaatcatgga ggacaggcgg ttggcttcca ccatgcggcg
     4021 gatgattttt ccaggtccga ccagatgctc ctgaccgatg acctggtcga tggttttagg
     4081 gcgcatgcga agcgcgagat tgtctggcat agcagtcctt tctaacgtgg atttgagtag
     4141 aatgactcat ctactatatc ttactatgtc cattctagcc tattagttgt gatatttcaa
     4201 tggttttgta ataatcaggg agtgagatag aatctttttt actatttaat ttaatgagga
     4261 gaggtatcag catgagagat ttgattttcc ttaaagctca aaataggata gttgagccta
     4321 aagaagtgga agtgctgact tgagaaataa tattgctgaa tgtcattgta tgaactataa
     4381 tgaaaagaga ggtgataagc aatggaaatg atggaccaag tcaggaaatt ttgatttt
//
LOCUS       DAVBHS010000142         4374 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVBHS010000142 DAVBHS010000000
VERSION     DAVBHS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.142"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..137
                     /locus_tag="VQ651_001780"
     CDS             <1..137
                     /locus_tag="VQ651_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choline-binding protein D"
                     /protein_id="HET2754736.1"
                     /translation="ASGAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYKVNANGEWV
                     "
     gene            538..1659
                     /gene="mnmA"
                     /locus_tag="VQ651_001781"
     CDS             538..1659
                     /gene="mnmA"
                     /locus_tag="VQ651_001781"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET2754737.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1800..2255
                     /locus_tag="VQ651_001782"
     CDS             1800..2255
                     /locus_tag="VQ651_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2754738.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELDVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            2265..4178
                     /gene="mnmG"
                     /locus_tag="VQ651_001783"
     CDS             2265..4178
                     /gene="mnmG"
                     /locus_tag="VQ651_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET2754739.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
BASE COUNT         1263 a          887 c         1064 g         1160 t
ORIGIN      
        1 aagcatcagg tgctatgaaa gcaagccaat ggttcaaagt atcagataaa tggtactatg
       61 tcaatggttt aggtgccctt gcagtcaaca caactgtaga tggctataaa gtcaatgcca
      121 atggtgaatg ggtttaagcc gattaaatta aatcatgtta agaacatttg acattttaat
      181 tttgaaacaa agataaggtt cgattgaata gatttatgtt cgtattcttt aggtacctat
      241 cttatgattt caggaaatgt cattaaaaaa acgactcctt ttctctaact tgaaaaatag
      301 attagagaaa atgggttgtt ttatttatta tagttatttg aatgaagata agaagaaggt
      361 atactcacat cattcacata atctgtatgt tgactataag tttaaaaaaa caatttttaa
      421 gctcttcctt gtcttctcca accaagcgtg ttataatgaa tactgctcaa gcgaccttca
      481 atcgtgaagc acacacgacc ttcaatcgtg aataaacgaa tagatgggag acttaccatg
      541 agtgataact ctaaaacacg tgttgtcgtg gggatgagtg gtggtgttga ttcgtcggtg
      601 acggctcttt tgctcaagga gcagggctac gatgtgatcg gtatcttcat gaagaactgg
      661 gatgacacag atgaaaacgg cgtctgtacg gcgaccgaag attacaagga tgtggttgcg
      721 gtggcagacc agattggcat tccctactac tctgtcaatt ttgaaaaaga gtactgggac
      781 cgcgtttttg agtatttcct agcggaatac cgtgcagggc gcacgccaaa tccggacgtt
      841 atgtgcaaca aggaaatcaa gttcaaggcc tttttggact atgccatgac cttgggggca
      901 gactatgtag cgactgggca ttatgctcga gtggcgcgtg atgaggacgg cactgttcac
      961 atgcttcgtg gcgtggacaa tggcaaggac cagacctatt tcctcagcca actttcgcaa
     1021 gaacaactcc aaaaaaccat gttccctctg ggacatttgg aaaagcctga agtacgcaga
     1081 ctagcagaag aagcaggcct ttcgactgct aagaagaaag actcgacagg gatttgcttt
     1141 atcggagaaa agaactttaa aaactttctc agcaactacc tgccagctca gcctggtcgc
     1201 atgatgactg tggatggtcg cgatatgggc gagcatgcag gtcttatgta ctatacaatc
     1261 ggtcagcgtg gcggactcgg tatcggtggg caacacggcg gtgacaatgc cccttggttc
     1321 gttgtcggaa aagatctaag caagaatatt ctctatgtag gacaaggatt ctaccatgat
     1381 tcgctcatgt caactagcct agaagccagt caagtccact ttactcgtga aatgccagaa
     1441 gagtttacgc tagaatgtac ggctaaattc cgttaccgtc agcctgactc taaggtgacc
     1501 gtccatgtca aaggagacaa ggcagaagtt atctttgcgg aaccacaacg cgcgattaca
     1561 ccaggacagg cagttgtctt ttacgatggc gaagagtgtc taggtggcgg tttgattgac
     1621 aatgcttacc gcgatggaca agtttgtcag tacatttaga ttgacaaatt ttctcaattt
     1681 gctacaataa taacagcaat agaaatgatg gtcaaagctc atggatgttg caggcttttt
     1741 tgtcctgcac ttctttgtag ttttgactgt ttttgtgtcg tttaagggaa aggacaagaa
     1801 tgactcaaca agactttcgg acaaaagtag acaatacggt ttttggtgtt cgggctacag
     1861 ccttgattgt ccaaaatcac aagcttctag ttaccaaaga caagggcaag tattacacta
     1921 ttggaggtgc gattcaagtc aatgaaagca cggaagacgc ggtagtccgt gaagtgaagg
     1981 aagaactgga tgtcaaagct caagctgggc agctagcttt tgtggttgaa aatcgttttg
     2041 aagtggacgg cgtttcctat cacaatatcg agtttcatta tctggtggat ttgcttgaag
     2101 atgccccgtt gaccatgcag gaagatgaga aaaggcagcc ctgtgagtgg attgacttgg
     2161 ataagctcca gaatatccag ctagttccag cctttttaaa aacagcccta ccagattggg
     2221 aaggccaact aagacacatt catcttgagg aataggagag aaacatgact tatcatttta
     2281 ctgaagaata cgatattatt gtaattggtg cgggacacgc tggggttgag gcttccttgg
     2341 ccgctagccg tatgggctgt aaggtcctgc ttgcgaccat caatattgaa atgctggctt
     2401 tcatgccttg taatccctct atcggtggtt ctgctaaggg gattgtcgta cgtgaagtcg
     2461 atgccctcgg tggcgagatg gccaagacca ttgacaagac ttacatccag atgaagatgc
     2521 tcaacacagg gaagggacca gccgttcgtg cccttcgtgc gcaggctgat aaggaacttt
     2581 actctaagga aatgcgcaag acagttgaaa atcaagaaaa tctgaccctt cgtcaaacca
     2641 tgattgatga gattttggtg gaagatggca aggttgtcgg tgtgcgtaca gccacccatc
     2701 aagaatatgc tgctaaggct gttattgtga cgacagggac tgctctccgt ggggaaatta
     2761 tcatcggaga cctcaagtac tcatcaggtc ctaaccacag cttggcttct attaacctag
     2821 ctgacaatct caaggaactg ggtctcgaaa tcggtcgttt caagacagga acccctccac
     2881 gtgtcaaggc ttcttctatc aattacgatg tgacggaaat tcagccagga gacgaagtgc
     2941 ctaatcattt ctcatacact tcacgtgatg aggattatgt caaagatcaa gtgccatgct
     3001 ggttgaccta taccaatggt accagtcatg agattatcca aaacaacctc caccgtgcgc
     3061 ctatgtttac aggtgtggtc aagggagtgg ggcctcgtta ctgtccgtcg attgaagaca
     3121 agattgtgcg ctttgcggac aaggaacgtc accaactctt ccttgagcca gaagggcgca
     3181 atactgagga agtctatgtt caaggacttt caaccagtct gcctgaggat gtccagcgtg
     3241 acttggttca ttccatcaaa ggtttggaaa atgcagagat gatgcggaca ggttatgcta
     3301 ttgagtatga tatggtcttg cctcatcagt tgcgtgcgac tttggaaacc aagaaaatct
     3361 caggtctctt cactgctggt cagacaaatg gaacatcagg ttatgaagaa gctgctggcc
     3421 aagggattat cgcgggtatc aatgcggctc tgaaaatcca aggtaaacct gagttgattc
     3481 taaaacgaag tgacggttat atcggggtga tgatcgacga cttggtgacc aagggaacca
     3541 ttgaacctta ccgtctcttg accagtcgtg ctgaataccg tctcattctt cgtcatgaca
     3601 atgctgatat gcgcttgact gagatgggac gcgagattgg ccttgtggat gatgaacgct
     3661 gggctcgttt tgaaatcaag aaaaatcaat ttgataatga gatgaaacgc ctagacagta
     3721 tcaaactcaa gccagtcaag gaaaccaatg ctaaggttga ggaaatgggc ttcaagccgt
     3781 tgacagatgc ggtgacagcc aaggaattcc ttcgccgtcc agaagtttct taccaagatg
     3841 tggtggcctt catcggacca gctgcagaag acttggatga caagattatc gaattgattg
     3901 aaacagaaat caagtacgaa ggctatattt ccaaagccat ggatcaggtt gctaagatga
     3961 aacgtatgga agaaaaacgc attccagcca atatcgactg ggatgacatc gattctattg
     4021 cgacggaagc tcgtcagaag ttcaaactca tcaatccaga aaccatcggc caagccagcc
     4081 gtatttcggg agtaaaccca gcagatattt ctattttgat ggtgtatctg gaaggtaaaa
     4141 atcgtagtat ttctaaaact cttcaaaaat caaaatgata cgtcgtcggc ttcttacgaa
     4201 tgagttcaaa gcttggcttt gattcatctc caacctccca tagttccccg aactatggga
     4261 gctaactcaa gttatgcatt ttcctagcct tctggtctga ttttcattga gttttaatct
     4321 caaaagaaag catagagaaa aacagctccg aagacggggc tgttttgttg actt
//
LOCUS       DAVBHS010000143         4368 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVBHS010000143 DAVBHS010000000
VERSION     DAVBHS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.143"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(1..1011)
                     /locus_tag="VQ651_001784"
     CDS             complement(1..1011)
                     /locus_tag="VQ651_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HET2754740.1"
                     /translation="MSFLSKNGAGILACLLISIVSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            1160..2329
                     /locus_tag="VQ651_001785"
     CDS             1160..2329
                     /locus_tag="VQ651_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2754741.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2326..3096
                     /gene="recO"
                     /locus_tag="VQ651_001786"
     CDS             2326..3096
                     /gene="recO"
                     /locus_tag="VQ651_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET2754742.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3093..4085
                     /gene="plsX"
                     /locus_tag="VQ651_001787"
     CDS             3093..4085
                     /gene="plsX"
                     /locus_tag="VQ651_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET2754743.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4091..4324
                     /locus_tag="VQ651_001788"
     CDS             4091..4324
                     /locus_tag="VQ651_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET2754744.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQSVGDVVKIIQGK"
BASE COUNT         1269 a          835 c         1118 g         1146 t
ORIGIN      
        1 ttagaaaatg ccgataaggg tctgcatacc aagagtggtg aggatgatgg caatccagca
       61 gatggctcca agaagaatgg attttccact ggatttgacc atagcgacca gattggtttt
      121 gagaccgata gcactcatgg ccatgacaat aaggaattta gagagttctt tgagaggggt
      181 aaagaaacta ctggacacac ctagagaggt gagtagtgta gtgaggagag aggcaaggat
      241 aaagtaaagg ataaaaagtg ggaagacttt tttcagttgt aggctttgct tgttttcttg
      301 ttggcgactt tgccagtagg agagaaagag cgtgataggg ataatagcca aagtacgtgt
      361 gagtttaaca atggttgcag actcgagggt attgctttgg taaagactgt cccaagcgct
      421 ggcggcagcc gttacagagg aagtgtcgtt gaccgcagtc cctgcaaaga gggcgaagcc
      481 ttcattggat agatgaagcc aggtgccgag ggttggaaag atgagcgcag ccaagacatt
      541 gaagaaaaag ataacggaaa tggcttgggc tacttccttt tccttagcat caataacggg
      601 cgctgtcgct gcaatggcag aacccccaca gatagaagaa cctactccaa ccaaggtagc
      661 cagttttgta tccagtgcaa agaaacgctg gaagaggtag gcaataatca gagctattga
      721 gatagtggac aggatgacag ggagtgaaga ttggccaact gcgaagacct gcgagatatt
      781 gagaccaaaa ccaagcaaga caacggcata ttggagcaac ttcttggaac taaaggtcaa
      841 accagcatcc agttgtttat aggacgagag aaagggatgt aggagcatgc ctatgaaaat
      901 ggcaaaaacg ggcgcgccaa tcacagggaa gaatcctcct aagtaccaag atacgataga
      961 aatgagaagg caggccaaga tacctgctcc attttttgat agaaatgaca taaaaacctc
     1021 caaataaaat ctattaccat tatagaccta taaacaggaa aagtaaaata gaaagtggaa
     1081 agctattctc taatgtattt ttgcggtcgg ggggcttttg tagtataata gagatacgtt
     1141 ttgaaagtag gaggtatcta tggacttaac taagcgcttt aataaacagt tagataaaat
     1201 tcaagtttcg ttgattcgtc agtttgacca ggttatttcg gagattcctg gggtcttgcg
     1261 tttgaccttg ggggaacctg attttacaac gccagaccat gtcaaggagg cggccaagcg
     1321 agcgattgat cagaaccaat cctactatac agggatgagt ggtctgctga ctctacgtca
     1381 ggcagccagt gactttgtta aggaaaagta ccaactggac tatgctcctg aaaatgaaat
     1441 tttggttaca attggggcga cagaggcttt atctgcgact ttgacggcta ttttggaaga
     1501 gggagacaag gtacttttgc cagctcctgc ttatccaggc tatgaaccga ttgttaactt
     1561 agttggggca gaaattgttg agattgatac gactgaaaat ggttttgtct tgactcctga
     1621 gatgttggag aaggccattt tggagcaggg tgataagctc aaggcggtta ttctcaacta
     1681 tccagccaat ccgacaggaa ttacctacag tcgagagcag ttagaggcct tggcagctgt
     1741 tttacgcaag tacgaaattt ttgttgtctg tgatgaggtt tactcagaat tgacctacac
     1801 aggcgaagcc catgtgtctc taggaacgat gttgagagac caggctatta ttatcaatgg
     1861 tttgtctaaa tcgcatgcca tgacaggttg gcgtttgggg ctgattttcg ctcctgcggc
     1921 cttcacagcc cagttaatca agagtcacca atacttggtc actgccgcaa ataccatggc
     1981 gcaacatgct gcggtagaag ccttgacggc tggtaaaaat gatgcggagc ccatgaagaa
     2041 ggaatatatc caacgtcggg actatatcat cgaaaaaatg actgctcttg gttttgagat
     2101 tatcaaacca gacggtgcct tctatatttt tgctaaaatt ccagcgggct acaatcaaga
     2161 ctcctttgct tttctgaagg attttgctca gaagaaggcc gttgccttta tccctggtgc
     2221 agcctttgga cgttacgggg aaggctacgt ccgcctatct tatgcagcca gcatggagac
     2281 tatcaaagaa gccatgaaac gacttgagga gtacatgaga gaagcatgat tcagtctatc
     2341 acgagtcaag gcttggtgct ttacaatcgc aattttcgtg aggatgacaa gctcgtcaaa
     2401 atttttacag agcaggttgg caaacgcatg ttttttgtca aacacgctgg tcagtctaag
     2461 ctggcgcctg ttattcagcc cttggtgctg gcacgatttc tcttgcgaat caatgatgac
     2521 ggactcagtt acatcgaaga ctatcatgag gtcatgactt ttcccaagat taatagtgac
     2581 ctctttgtca tggcctatgc gacctatgtg gcagctcttg cagatgctag tttgcaggac
     2641 aatcagcagg atgctccctt gtttgctttt ttgcaaaaga ctttggagtt gatggaagca
     2701 ggcttggatt atcaggtttt gaccaatatt tttgaaattc aaattttgac tcgatttgga
     2761 atcagcctca attttaatga gtgtgtcttc tgccatcggg ttggtcaggc ttttgacttt
     2821 tctttcaaat atggagcctg cctctgtcca gagcattatc atgaggataa gagacgttgt
     2881 catctcaatc ccaatatccc ctatctgctc aatcaatttc aagctattga ttttgagact
     2941 ttggagacca tttcgctcaa gcctggaatc aagcaagagc tacgccaatt tatggatcaa
     3001 ttatatgaag agtacgttgg gattcaccta aaatcaaaga aatttattga ttccctagca
     3061 gactggggac aattactaaa agaggaaaag aaatgaaaaa aatcgcagta gatgccatgg
     3121 ggggcgatta cgcacctcag gccattgttg agggtgtcaa tcaagcccta tctgactttt
     3181 cagatatcga ggttcaactt tacggagatg aagctaaaat caagcaatat ctgacagcga
     3241 cagagcgcgt cagcattatc catacggatg agaagattga ttcggatgat gaacctacga
     3301 gagctattag gaataagaaa aatgccagta tggtattggc agccaaggct gtcaaagatg
     3361 gtgaagcaga cgctgtcctt tcggctggga atacaggtgc cttgttggca gcaggattct
     3421 tcatcgtggg tcgtatcaag aatatcgacc gtcctggact catgtctacc ttgcctaccg
     3481 ttgatggaaa aggttttgac atgctagacc ttggtgccaa tgcagaaaat acagcccagc
     3541 acctccatca atatgcggtt ctaggttcct tctatgctaa aaatgtccgt ggcattgcgc
     3601 aaccacgcgt tggtttgctc aacaacggaa cagagagtag caagggcgac ccgcttcgta
     3661 aggaaactta tgaattactg gcggctgatg aaagtttgaa ctttatcgga aacgtggaag
     3721 cgcgtgattt gatgaatggc gttgcagatg ttgttgtggc agatggtttc acgggaaacg
     3781 ctgtgctcaa atccatcgaa gggacagcta tgggaatcat gggcttgctc aagacagcta
     3841 ttacaggtgg tggtcttcga gcgaaactag gtgccctcct tctcaaggac agcctcagag
     3901 gtttgaaaaa acagctcaat tattcagatg ttggtggagc ggtcttgttt ggtgttaagg
     3961 cacctgttgt caagactcat ggctcaagcg atgccaaggc tgtttatagt acgattcgtc
     4021 agatccgtac catgctagaa acagacgtgg ttgcccagac tgcgcgtgaa ttttcaggag
     4081 aataaaagag atgacagaaa aagaaatttt tgaccgtatt gtgaccatta tccaagagcg
     4141 acagggagag gactttgtcg tgacagaatc cttgagtctg aaagacgatt tggatgcgga
     4201 ttctgttgac ttgatggagt ttatcttgac tctggaagat gaatttagta tcgaaatcag
     4261 cgatgaggaa attgaccaac tccaaagtgt aggagatgtg gttaaaatca ttcaaggaaa
     4321 atagcaatcg gagttccaag tcaacggaag tagatggttt ttagaaat
//
LOCUS       DAVBHS010000144         4349 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVBHS010000144 DAVBHS010000000
VERSION     DAVBHS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.144"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            73..2439
                     /locus_tag="VQ651_001789"
     CDS             73..2439
                     /locus_tag="VQ651_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HET2754745.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLSKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTHLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(2487..3770)
                     /locus_tag="VQ651_001790"
     CDS             complement(2487..3770)
                     /locus_tag="VQ651_001790"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET2754746.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     EDGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(3964..4323)
                     /locus_tag="VQ651_001791"
     CDS             complement(3964..4323)
                     /locus_tag="VQ651_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2754747.1"
                     /translation="MDSPKSLASYRVIGISDRVLEILKEYLLLEEGDYIFTNNGSPLM
                     ITCFNYFLKNRFRKSEIKKDDFVLTAHVFRYSHISLLAELEVPITAIMDRVDYTNETK
                     ILSVYTHVTEKMKSNIT"
BASE COUNT         1263 a          952 c          823 g         1311 t
ORIGIN      
        1 gtttgaagat attttcgaag agtataaaat aaaaacaatc ccaactctca cctattcatg
       61 caaaggaatt ttatggaagt ttatttttca ggaaccattg aacggattat ttttgaaaat
      121 cccagcaatt tttatcgcat cctcctccta gaaatcgacg atacggacgc agaggatttt
      181 gatgattttg aaatcattgt cacaggaacc atggctgatg taattgaggg cgaagactat
      241 actttttggg ggcaaattgt ccagcactcc aagtatggag aacaactgca aatcagtcgt
      301 tatgatcgcg caaaaccaac tagtaagggc ttggtcaagt acttttcaag tagccatttc
      361 aaggggattg gtctcaagac agctcagaaa atcgtggata cctatggcga aaataccatt
      421 gacgaaattt tgcaacaccc agaaaagtta gaaggcatcg caggactctc tgccaaaaat
      481 cgcgaggctt tcgtctccac tctccgtctc aactacggaa cggagatggt tttgtccaaa
      541 ctagccaact acggcattcc caacaaacta gcctttcaga ttcaagactt ttacaaggaa
      601 gaaacccttg atgtggttga aaattatccc taccagctgg ttgaggatat caagggtttg
      661 ggctttacca ttgctgacca actagctgag gaactaggca tcgaaagtca ggctcctgaa
      721 cgcttccgcg ccggtctagt tcacagtctt tttcaggcct gtatggaaac aggggacacc
      781 tatgttgaag cacgggattt gctggaacaa acccttactc tccttgagtc ttcccgtccc
      841 gtggaactag accccagcca agttgcccaa gagctctcct acctgatcga agaagacaag
      901 gttcagcaga ttgataccaa gatctttgac aacagcctct ttttcgctga ggaaggcatc
      961 cgcagtcact tggttcgtat ccttgaaaaa ggaaaacaga agagtcatga tttagaaacc
     1021 attcaaaaac atatcactac tatcgaggaa gagctgggaa ttgagtatga taacattcaa
     1081 aaacaggcta tctgtgatgc tatccagaat aaggtcttta tcctgacagg tgggcctggt
     1141 actggtaaga caactgttat caatggaatc atcgctgttt atgccctgtt agagggactt
     1201 gacttcagga agaaaagcaa tctgcctatt cttcttgctg ctccaactgg tcgagctgct
     1261 cgtcgcatga atgaattgac aggtttgcct agcgcgacca tacatcgcca cttgggaatg
     1321 acaggtgacg atgataccag tcatctggaa gattacctag atgctgactt tatcatcgtg
     1381 gatgaattct ctatggtaga tacttggctg gccaatcaac tcttctccaa tatctcttct
     1441 aacagtaaga tcctcatcgt aggtgacagt gatcagctac cttctgtcag tcctggacag
     1501 gttctagcgg atctgcttca tattcctttg attcctcaga ctcacttgga aaaaatttat
     1561 cgacaaagca aagaatcaac catcgtcacc ctagctagtc agattcgaca gggcatcttg
     1621 ccagctgatt tcacccaaaa aaaagctgac cgttcctact ttgaaattgc tagtggccat
     1681 attcctgcca ccattgaaaa aatcttaggt gccgccctca gaaatggtat tcctgcccgt
     1741 gatatccaag ttctggctcc tatgtaccga gggacggcag ggattgatgc tatcaatcag
     1801 ctcatgcaag acctgctcaa tcccccacaa aaagatcaac tcagttttga agctcctcag
     1861 tgccactatc gtaagggaga caaggtcatt catttggtta acgatgctga aatcaatgtc
     1921 tttaatgggg atttaggagc tatcacagac ctgattcctg gtaaatacac cgaatcgaaa
     1981 caagacgaga ttgtcattga ttttgatggt aatgaagtct cttacccccg taacgaatgg
     2041 tacaagattc gcttggccta tgccatgagt attcataagt ctcagggaag tgagttccca
     2101 gttgtcatcc taccgattac tagtgctagc cgacgtatgc tggagcgaaa tctcatctac
     2161 acagccatta cacgtgccaa aagcaaactc atcttactag gcgaattaca agccttcgac
     2221 tatgctaccc aacacatcgg aactgcccga aaaacctatc tgattgaacg cttcagtgat
     2281 ctactggaga atgttgaaga aaagcaacaa gctgtctctg aaacagtcac atcaagtgcc
     2341 tctgaacaat cctacatcct aaccgaagaa aactgggaca gcattccagc catgattggg
     2401 attacagaca cagacctcaa agagattttt ggaaaatagt gcatcagaaa aaccaccaat
     2461 tcaggtgggt tttctgttta ttaagattat tttactgttg ctgtgcttgt aatcaattca
     2521 acagcttttt tgatagtgat atcatgtttc aacatgtcag ctgaaagcaa gttttgaact
     2581 tgtgcaactt ccatgttgta gtctgctgcc aattgctcaa cttctttttg gatttcttct
     2641 tctgaagcat caaatccttc agctttggca actgcttcga taacaaggtt agtcttagta
     2701 cgtgactcag cttctgcttg gtattggttg tgaaggtctt cttgagtagt tccagtaatt
     2761 tggaagtaca tgtcagggtt gatcccttga cgttgcaagt tcccaaggaa ttcatttact
     2821 gaacggtgaa cttcttcgtg gatcatttct tctggaagtt ctacgatttc agcattttct
     2881 acagctgtat caattgctgc accttcaact gcatctttgt aagcttcttc tttagcagca
     2941 gccaattctt tgcggtattt ttctttcaag tcagcaagtg tttcaacttc ttcatcaatg
     3001 tcttttgcaa gttcatcgtc aagagctgga acttctttag cttttacttc gtggatagtt
     3061 gtcacgaatt tagcttcttt acctgcaagg tcttctgctt ggtagtcttc tgggaatgtt
     3121 acgataacat caacggtttc gccagctgag tgacctacca attggtcttc gaaaccaggg
     3181 atgaattgac ctgaaccaag tccaagtgag aagttttcac cttttccacc gtcaaattca
     3241 acaccgtcga tagaaccaac gaagtcgatc acaacagtgt cgccgtcttc agcagcagct
     3301 tccttgataa ccaattcagc caggttgttg cgttcacgtt cgatacgctc ttcgacatca
     3361 gcgtcagtta cttctttttc tacatcaact gatacttcaa ggtttttgta gtcacccaat
     3421 tttacttcag gttttgtaac aacttcagca gcgataaccc agtcttgacc tttttccatt
     3481 gaagttacgt caatttttgg ttgggcaacc acttcaagac cagcctcttt tacagctgct
     3541 tcataagcgt ttggcaaaag agcgttcata acgtcttggt aaagtgattc ttcaccaaat
     3601 tttttatcga agataggacg tggaaggtga cctttacgga aacctggaac attaagagat
     3661 ttcttcactg acttgaagac acggtccaat tctggtttga tttggtcttg agagatagtg
     3721 aaagtcaaga caccacggtt tgtttctttg ttttcaaatg atacagacat tctgtcattt
     3781 ctccttaaaa ttttttaaat acagtctatt ataacatttt ctataaataa aatctagact
     3841 aatgcagaca attatagaca acaagccttt ttcctaacta aaacactcat aaagtataga
     3901 caattttaga ctactttgga tatcttttgg ttagtcattt tccaaaacta atttatctaa
     3961 tttttaagtt atatttgact tcatcttctc agtaacatga gtataaacac ttaaaatctt
     4021 tgtttcatta gtataatcta cacgatccat tatcgctgtt ataggtactt ccaattcagc
     4081 aagcaaactt atatgcgaat atctaaaaac atgagcagtc aatacaaaat catctttctt
     4141 aatttcactt ttcctaaacc tgtttttcaa aaagtaattg aaacaagtta tcattaaggg
     4201 agaaccatta tttgtaaata tataatcacc ttcttctaaa agaagatatt cttttaaaat
     4261 ttctaacact ctatcactaa taccaattac tctatatgaa gctaaacttt tcggactatc
     4321 caaaaacact tcgttattgg acgattaca
//
LOCUS       DAVBHS010000145         4298 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVBHS010000145 DAVBHS010000000
VERSION     DAVBHS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.145"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..611)
                     /locus_tag="VQ651_001792"
     CDS             complement(<1..611)
                     /locus_tag="VQ651_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HET2754748.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQL"
     gene            complement(720..1238)
                     /locus_tag="VQ651_001793"
     CDS             complement(720..1238)
                     /locus_tag="VQ651_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HET2754749.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1465..2334)
                     /gene="rsmI"
                     /locus_tag="VQ651_001794"
     CDS             complement(1465..2334)
                     /gene="rsmI"
                     /locus_tag="VQ651_001794"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET2754750.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGTSAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2337..2654)
                     /gene="yabA"
                     /locus_tag="VQ651_001795"
     CDS             complement(2337..2654)
                     /gene="yabA"
                     /locus_tag="VQ651_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HET2754751.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(2694..3584)
                     /locus_tag="VQ651_001796"
     CDS             complement(2694..3584)
                     /locus_tag="VQ651_001796"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET2754752.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(3581..4219)
                     /locus_tag="VQ651_001797"
     CDS             complement(3581..4219)
                     /locus_tag="VQ651_001797"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET2754753.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
BASE COUNT         1192 a          978 c          685 g         1443 t
ORIGIN      
        1 tcaagctgtg agttcattat gatttgtaaa tccgatatat cttcagcagc taaaaacaca
       61 ttttgaaatg ttccaagtcc gttttcacca aaactatatt ctctcttact atacttactc
      121 ttattattct ctatatagtt agagtcaagg ttcttaacct cttgaagttc attttcttta
      181 cttctgaggt taaaatcttt tacttctgaa gttaagtttt ttgacttctg taagtcattt
      241 tcttttacct gaaatatact cataaagtct ttaacataaa tgatgttcgg cttaccaagt
      301 ccaagcctta ctctttcgat cagtcctatt ccttttttta catcaagctc atctaatgtt
      361 tttatggcag ttggctttga gatatttctt cttttcataa tttcttcgac agtaaaatag
      421 ataaatactc tgccttcctt gtctatccag ttattcttaa atgacattcc tgtctgttta
      481 agaagtatgg aataaaggat aattgcttct gctgataagc cccgaaattc ttctctatca
      541 actaatatct ccggtacttt taagaagtta aatctttccg cttctctgtt ataaaaatat
      601 tcaaagtcca tgcaagatca cctcccttct taaaaatttg caaagaaaaa gacgatagtt
      661 tttcttctat cgcctttggt aaccattttt atgaaatttt ttaaagttaa aaatataatt
      721 tataatacta tcttcttcca ttttctgatt ttagttctga aagttccaaa aggtgcaacc
      781 gtatttacat gaataaactt atacacttcc caagtcgctg ttttagtcgc ttcatcagcc
      841 cactttctca tatgcggttc aaataattct tcctcactca acgaatcaat cattgcagag
      901 atagaattaa tattttcatt taattttgct ttcaactctt gcagagataa atgagcgtag
      961 gtatctgtga accactgata taattcacca agttgattcc atttaaattt atccgatggt
     1021 gtttttactt gaagcccctt tctttcatct tcttcccatt taagaaccaa gttggtccaa
     1081 ccaacctgat aagcaaggtt ttctgctgga gttctgtcaa cttcatcagc tctcttatct
     1141 tttaaatttt ctggaatatt atcaaattct aaaatatatt tctcaaatgt tttctctatc
     1201 tcagctttta gttcttcttt attttcgtat gttcgcaaaa aatcaccttc tttctgattt
     1261 atcaatcctt tgatttaaaa tgaccttatt tttctattta taccttctaa gaacgctata
     1321 actgctgctc caaacatcgg tattccgtat gggcttaata aaaaacttaa taccaaagct
     1381 tctataccaa gtgatacatc tttatattat catcactaat taagttaaac agaaacagac
     1441 agaattatta catcctgtct ccctttattg tttttcttcc caatcgtggt aggcagcgta
     1501 gagttgactc ttattccact ggtaaatctt agctacttcc ttaatagctt gatttttctt
     1561 catgccttgc tggatacggg cttggatttc taagaacaag tcctcctcgt ctttttcctc
     1621 cacatcctga ctggcacctt caacaataag aaggcattcg cccttgagtg gcgtttcagc
     1681 gatgctttcc agtaattcag aaattgtacc tcgttggtat tcttcataga ttttggtcaa
     1741 ttccctgacc aaaacaaccg agcggtcacc gtagacttct aacatatttt ccaacgtgtc
     1801 tgctacacga tgaggtgatt cataaaaaat ctgtgtttca ggataatctt ttttagagcc
     1861 aaaaaattgc ttctgttgac ctgattttct cggtaaaaaa ccgtaaaaga tatgtggctg
     1921 tggcgctaaa ccactggcaa tcaaggcaga aattcctgca gaggtacctg gaacagtcac
     1981 aactgcaatt tcttcctcaa tagctgcctt aactaaatca tgaccagggt ctgaaatgct
     2041 aggcaaaccg gcatcagaga cctgagcaat actttgccct gctttcaaga aaccaatcaa
     2101 atcaggaatt ttttccttgg cattgtgctc atgaaaactg atctgcttgg tggaaatgtc
     2161 aaaatgcttg agcaaaagcc ctgtattgcg cgtatcctca gcagcaatcc agtccacttc
     2221 tttcaaggtc tggatagcac gaaaagtcat atcatctaga ttgccaatcg gcgttgccac
     2281 tagatacagc ttgccatagg gagactgccc cttaaaactt ttttgaattt gcatgcttac
     2341 tccctgtata acaactcatc acagaacata cattcttcgt cctgctctcg acgttgtcca
     2401 taaaaatcat tacatacgtg aaatccatca cggtaaatac gacggacact ttcgcgaaca
     2461 tgcttggcct tgacgggagc gtctgcttcc acctcaccca agcgttctcg caacttacta
     2521 ttttccaagc gaagagctgt attttcttct accaagctct tgagattttt cttgatggct
     2581 tccacatcgg ctaaagttac caataattgt tgggaaaaat catccagcgc gtcaaataat
     2641 tcttttttgt ccataaaaca gccctttcct ttctttatca ttcatttttg agtttatatt
     2701 tctttcaaga ccagatattc catggcattt tgaaagctga cattagcttg ccacattttt
     2761 ctagcttcta gtaaatcttg tagaatcact cttactctta cctgcaagag gtcctgccca
     2821 cagagaactt caagaatccg taaaacctga tcctgttttt ccttatcatc tgccaagttg
     2881 gctaatttgg caacctgtag ataactttct tttttcttag ctactaacca agtcagcagg
     2941 cgttcacttt catcgaccaa ggtccaaaaa cttgcctgat tagccaactt ttctgcttca
     3001 gctcgcgatt gactaaactt agctaaaaga gtcgcttttt tcttaacaag tcccatttgt
     3061 tctaagagta agataagttt ttcttcttgc tttttaaagt ggaagatctg agtccgactt
     3121 cggattgtcg gtaacatctt ttcctcatcg ctagtcaaga agaaaatata aacttcactc
     3181 tggggttctt cgatgacctt gagcagagaa ttggctgcgt tgggatgcat tttatccgct
     3241 tgctcgatga taaagacctg ttgctggctt tcaatccctg cttgagaaaa ctgtcccacc
     3301 aattcccgaa tacgttctgt cttgatgacc tgattgactg gcttaatcaa ggtgacatct
     3361 ggaaactctt cctgttcaat cagcttgcaa cttcggcatt tctcacatgg taagacgcca
     3421 actttatccg tacaaaagag gctcttagct aaaaattgcg ccatttccaa gcttccaaag
     3481 aaacctgaaa agagataggc gtgattgagc tggtcttgtt ctaagatacg gacaaaacgg
     3541 tcaaactgag ctggttgcca agcctttagt tgatcttgtt tcatttggcc aagcccattc
     3601 cgtcaaacaa gacagccttg gtagtttcca caacttgctc caaagggaga ctagcatcaa
     3661 tcttgacaat gcgatttccc tctttatcca gaagagaaag gtagccttga cgaacttttt
     3721 tatgcaagtc caacccttcc aaatccaaac gattaacctc gcggtcacta ttagcagcaa
     3781 tacgagccag cccttcttcc acctcgatgt caaaatagag tgtcaaatcg ggtttgaggc
     3841 catctgtcgc aaactgattg agccagtcaa tggcttcaat atctaagcca cgaccaaatc
     3901 cctgataggc aacagaacta tcgataaaac gatccatgat gaccaacttg ccagcttcaa
     3961 gggctggaag aactttttcc accaaatgct gtctgcgact ggcaatatag agaagtagct
     4021 ctgttttagc atccatctga gtatgacttg gatccaaaat cacttcccga atcttctccc
     4081 caatcaagac tccgccaggt tcacgggtcg tcagcacctt tactcctttt tcctctaaaa
     4141 ttggtagcag agcctctaaa acactggtct tgcctgctcc ctctggtccc tcaagagaga
     4201 ctaaaaatcc ttttgacatg tctaactcat ttctttttta ctacttctat tctatcaaaa
     4261 aaataggggt ttgtgacaat ctttttgtct tctcattt
//
LOCUS       DAVBHS010000146         4245 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVBHS010000146 DAVBHS010000000
VERSION     DAVBHS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.146"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..1288)
                     /locus_tag="VQ651_001798"
     CDS             complement(<1..1288)
                     /locus_tag="VQ651_001798"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET2754754.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(1366..2613)
                     /locus_tag="VQ651_001799"
     CDS             complement(1366..2613)
                     /locus_tag="VQ651_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET2754755.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGVSIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVSVIISSLTGNDYFGKQSLPRLMMWETVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(2804..3654)
                     /gene="rgg"
                     /locus_tag="VQ651_001800"
                     /pseudo
     CDS             complement(2804..3654)
                     /gene="rgg"
                     /locus_tag="VQ651_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398326.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes.; frameshifted;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
     gene            3778..4119
                     /locus_tag="VQ651_001801"
     CDS             3778..4119
                     /locus_tag="VQ651_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET2754756.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
BASE COUNT         1351 a          891 c          829 g         1174 t
ORIGIN      
        1 attgagtata aaatcctagt ttttcaaaga tttctgagaa gttttggctg attgtctcaa
       61 gtgacacttg cacttcttct cgggtttggt tgttcttgac cgtcacttgt ccgctttcga
      121 cttcgctctc tcctagggtg atgagggtct tagccgcaaa gacatcggct gacttgaact
      181 gagcttttag tttacggttg aggtaatcac gctctgcttt gaaaccttgt tggcgaagag
      241 cctgtaccaa ttccaaggcc ttgatatttg ccccttcgcc caagactgcg atatagacat
      301 ctagggcgtt ttcgataggg agggtcacac cttgcttttc aaggatgaga agcaggcgct
      361 ctacaccaag tccaaaacca aatccagcag tttcagggcc tccaaagtaa gcaaccaaac
      421 catcgtagcg accacccgca cagacggtca ggtcattgcc ctcaatctct gtgataaact
      481 cgaaaatggt gtggttgtag tagtccagac cacgcaccat attggtatcg atgatgtaat
      541 ctactccaag attttccaac atctgacgca cagcatcaaa atgagcttgg ctttcttcat
      601 caagaaagtc caagatagac ggcgcattct ctactgccac cttgtcttct ttttccttag
      661 agtccaagac acgaagagga ttttcctcca agcgacgttg gctatcctta gacaaggtct
      721 ccttgagcgg tgtcaaatag tcaatcaagg cttggcggta ggctgcacgg ctctcaggat
      781 ttccaagagt gttgaggtgc aatttgacac cttgaatacc gatttccttc aaaaaatggg
      841 ctgccatagc gattgtttcc acatcggtag ctggattgct agagccaaaa cactcaacac
      901 caatctggtg gaattggcgc aagcgccctg cctgtggacg ctcataacgg aacataggtc
      961 ccatgtagta gaacttgctt ggcttttgca cttctggggc gaaaagttta ttttccacat
     1021 aggaacggac aacgggtgca gttccttctg gacggagggt aatatgacgg tcacccttgt
     1081 cataaaaatc gtacatttcc ttggttacga tatccgttgt atctccgaca gagcgactga
     1141 taacctcgta atgctcaaaa ataggcgtgc gcacttctgc atagttgtag cgtttgaaaa
     1201 tctcacgggc aaagccctca acgtactgcc acttagcaga ctcagcaggt aaaatatcct
     1261 gcgttccttt tggtttttgt aatttcatag ggaatcctct ttaaacttaa tagtcttatt
     1321 ttaccataaa tagagggatt aaaacagtaa gaaaaaaatt aggatttaga tatcattttt
     1381 gagattaaga attgtcaaaa aaatagctag caaggaaaga ccaacaaata gcatccaagt
     1441 caactgtata ttccatacgg ctactagtga aaaacaagct gttcccacag gtatggataa
     1501 ggtaaacaat agacctaaaa aattactagt acgagctaga acctctggag ctagattttt
     1561 catgagcatg gcactaatct ttggttgaac tttaccagac acatacagag taaagaagag
     1621 aaatagcaaa ccaagcacga cttgattgaa taaattagcc aaaccaacta gactaagtcc
     1681 tacggtctcc cacatcatca atctaggcaa ggactgcttc ccaaaataat cattgcccgt
     1741 aaggctactg atgatgactg atactaaaac acagaattga ttgataaata gtgcctctgt
     1801 ataagaaaaa ttcaagagag aatggctcaa aaagaagata ttataaattc cacccaaagc
     1861 gccacccaag gaattaataa gcaagacagc aaagagcata aaaccaaagt ttttctgtcc
     1921 acttttaaga aaaacgagac gtaaatttcg gtaaattgtt aggaactggt ctttgataga
     1981 aagcttctca ttttttaagt tttcaccatc agcagatgac attgacaggc tcaatttgct
     2041 ttttcctaaa aagaggatag tggctgatac taggaaaaag caggcattga ttcccgcaac
     2101 gagagaaaaa ttgttgaccg atagagctaa gagccagact ccgaaagctt gaccaccaat
     2161 agctgaaata taggtgatga actgtgaaaa agaataagcc tccatcagat catcttcagc
     2221 tactttttcc ttaataagag gcatacgcag gccacctgca aaatcactga tgatatcact
     2281 aatgacattg atcaaacaca ggctagaaaa ggcaaagaga ctagcttgct gaacaactag
     2341 ggctgctaga aaaaatagaa ccgcctgaaa caaaccgcta tagaccatcc atttgacctt
     2401 gtccctcgtg taatctgccc gaatccctgc aaaaactgta aagagggtcg gaagaatcat
     2461 gacaatattc gccatagcaa cagcaaaaga tgcttgtgac aaggtcgatg catagacgat
     2521 aaagaccagg ttgaaaatcg aaacaccaaa agcattgaag aagcgtgata aagtcaaaat
     2581 tctataagct ggatttctaa acaatagttt catagataat ctccctcctt gttctaagtt
     2641 ctacatcaag aatagcttta gtatagcact tatgaatttg cgaaacgatt cttttgaata
     2701 tatgcaacaa acaaaatgag caaaaaaacg atagaacttt tgtaatgacc tgagagaggg
     2761 caacagttct atcgcttcaa attttttctt ggtttgcaga tattcaagaa tcgggagttt
     2821 ttctatagta ttcggcagat ttattacagc caagcatctc aaaaatacgg acagcatcct
     2881 ccatcttttt ctggccttcc ttgactctac cttgcttgct atcaaggaga ccttctgccc
     2941 acagataaac aattcggaaa taggtctcat tttccttgta gaaatgctct tcgataacac
     3001 gtttaaaata ataggcattg gtaaattctt cacactcaat actagctaaa aagccattca
     3061 atagtatagt atgaaaaagg tttcgattgc cagacatttc cattagaaaa tcggatttac
     3121 gtaccatttc tcgtacatat ctagtaaaaa gagaaacaga taaaaatgga gaactgactg
     3181 aaaataaatt gagttcatag attccccaga tctcggtaga aaacaaataa tcatgaagga
     3241 cttttccttc ctctgctgtt aagtctaccc tttcatctat gctcttcata taagacttga
     3301 taataatggc atttagaata tgtttctgtt tgttgtgaga atgggcatgc ttttatactc
     3361 cctgcgatat aagtcctcaa gaggtgctat attctttggt tccaagacat ctgtaatttc
     3421 ttttctcaac tcagaatctg tatcatactg gaaacctctt gccagaaaga ggatctcctc
     3481 cacactggca gatatatttt ccagagcaaa tagaaacttt tccaccgaaa gctcactctg
     3541 acctgtttca aaacgggaca acatagacgg cgaaaattgt cctccggttg cttgtctcag
     3601 tgagatattt cttgactctc gtaattgtct aaagactttt ccaatctgct ccatagactt
     3661 ccccttgatt ccgtattttc ttcattttat catatttttc agaaaattca tcaaaaactt
     3721 gccaaattgt cagaattatg agaaaataga ggatatttat cacgtggagg gactgctatg
     3781 agagacgata tcaaaatcaa tgaccgtgct ttggccttgc aagaccaaat tatcgaaaaa
     3841 ctagagaaag tttttgatac agatgtggaa ttggatgttt acaatctagg tctgatttat
     3901 gaaatcaatc tggatgaaac ggggctctgc aagattgtca tgaccttcac cgatactgcc
     3961 tgtgattgcg ccgaaagcct gcctattgaa atcgtggcag gtctgaaaca aatcgagggt
     4021 atcaaagata tcaaggttga agttacctgg tcgcctgctt ggaaaatcac acgaatcagt
     4081 cgctatggcc gtattgccct tggactacca cctcgttaag cagaccaatc acttttaaag
     4141 atgaaaaaag caagccgaaa atggcttgct ttttgatact caatgaaaat caaagggcaa
     4201 actagaaaac tagccgcagg ttgctcaaaa cactgttttg aagtt
//
LOCUS       DAVBHS010000147         4240 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVBHS010000147 DAVBHS010000000
VERSION     DAVBHS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.147"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            3..350
                     /locus_tag="VQ651_001802"
     CDS             3..350
                     /locus_tag="VQ651_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754757.1"
                     /translation="MLRLPYKKQKISHFTVSEDKDICRYIRQQLYYQNLFWMKEQAEA
                     YQKGENILTYGLKEWYPQIRPIVGKFFQIEQDLTSYYQHFYTYYQKNPQNDWQKLYPP
                     AFYQQYFLKNMVE"
     gene            369..2171
                     /gene="pepF"
                     /locus_tag="VQ651_001803"
     CDS             369..2171
                     /gene="pepF"
                     /locus_tag="VQ651_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET2754758.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEVLKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTFYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGIYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            2173..2886
                     /locus_tag="VQ651_001804"
     CDS             2173..2886
                     /locus_tag="VQ651_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET2754759.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELENFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            2953..3894
                     /gene="prsA"
                     /locus_tag="VQ651_001805"
     CDS             2953..3894
                     /gene="prsA"
                     /locus_tag="VQ651_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HET2754760.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(4011..>4240)
                     /locus_tag="VQ651_001806"
     CDS             complement(4011..>4240)
                     /locus_tag="VQ651_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2754761.1"
                     /translation="QASMSRKGNSQDNGRMESFFGILKSEMFYGYEKTFKSLNQLEQA
                     IIDYIDYYNNKRIKVKLKGLSPVQYRTKSFR"
BASE COUNT         1405 a          806 c          850 g         1179 t
ORIGIN      
        1 aaatattgcg tcttccctat aagaaacaaa aaatatctca ttttacagtt tctgaggaca
       61 aggacatctg tcgctatatc cggcaacaac tttattatca aaatctcttt tggatgaaag
      121 aacaagcaga agcctatcaa aagggagaaa atatcctgac ttatggactg aaagaatggt
      181 atccacaaat tcgaccaata gtgggcaaat ttttccagat tgaacaagac ttgactagct
      241 attatcagca cttttatacc tattaccaaa aaaatcctca aaatgattgg caaaagcttt
      301 atccaccagc cttttatcag caatatttct tgaaaaatat ggtagaatag aaaggatgga
      361 ggaatctaat ggtattacaa agaaatgaaa taaatgaaaa agatacatgg gatctatcaa
      421 cgatctaccc aactgaccag gcttgggaag aagtcttaaa agatttaaca gaacaattgg
      481 agacagtagc ccagtatgaa ggccatctct tggatagtgc ggataaccta ctagaaatca
      541 ctgaattttc tcttgaaatg gaacgccaga tggagaagct ttacgtttat gctcatatga
      601 agaatgacca ggatacacgt gaagctaagt atcaagagta ctatgccaag gccatgacat
      661 tctacagcca gttagaccaa gccttttcat tctatgagcc tgaatttatg gagattagcg
      721 aaaagcagta tgctgacttt ttagaagctc aaccaaagct gcaggtttat caacactatt
      781 ttgacaagct cttgcaaggc aaggatcacg ttctctcaca acgtgaagaa gaattattgg
      841 ctggagctgg agaaatcttt ggttcagcaa gtgaaacctt cgctatcttg gacaatgcgg
      901 atattgtgtt cccttatgtc ctagacgatg atggtaaaga agttcagcta tctcatggga
      961 tttacacacg tttgatggag tctaaaaaac gtgaggttcg ccgtggtgcc tatcaagctc
     1021 tttatgcgac ttacgaacaa ttccaacaca cctatgccaa aaccttgcaa accaatgtta
     1081 aggtgcaaaa ttaccgtgct aaagttcgta actacaagag tgctcgtcat gcagccctcg
     1141 cagcgaattt tgttccagaa agtgtttatg acaatttggt agcagcagtt cgcaagcatt
     1201 tgccactctt acatcgctat cttgagcttc gttcaaaaat cttggggatt tcagatctca
     1261 agatgtacga tgtctacaca ccgctttcat ctgttgaata cagttttacc taccaagaag
     1321 ccttgaaaaa agcagaagat gctttggcag tcttgggtga ggattacttg agccgtgtta
     1381 aacgtgcctt cagcgagcgt tggattgatg tttacgaaaa tcaaggcaag cgttcaggtg
     1441 cctactctgg tggttcttac gataccaatg cctttatgct tctcaactgg caggacaatc
     1501 tggacaatct ctttactctt gttcatgaaa caggtcacag tatgcattca agctatactc
     1561 gtgaaactca gccttatgtt tacggagatt actctatctt tttggctgag attgcctcaa
     1621 ctaccaatga aaatatcttg acggagaaat tattggaaga agtggaagac gacgcaacac
     1681 gctttgctat tctcaataac ttcctagatg gtttccgtgg aacagttttc cgccaaactc
     1741 aatttgctga gtttgaacac gccattcacc aagcagatca aaatggggag gtcttgacaa
     1801 gcgatttcct aaataaactc tacgcagact tgaaccaaga gtattatggt ttgagtaagg
     1861 aagacaatcc tgaaatccaa tacgagtggg ctcgcattcc acacttctac tataactact
     1921 atgtatatca atattcaact ggctttgcgg ccgcctcagc cttggctgaa aaaattgtcc
     1981 atggtagtca agaagaccgt gaccgctata tcgactacct caaggcaggt aagtcggact
     2041 atccacttaa tgtcatgaga aaagctggtg ttgatatgga gaaggaagac tacctcaacg
     2101 atgcctttgc agtctttgaa cgccgtttaa atgagtttga agcccttgtt gaaaaattag
     2161 gattggcata aaatggttga atcgtatagt aagaatgcta accataacat gcgtcgtcct
     2221 gtcgtcaaag aagaaattgt agacttgatg cgtcagcgtc aaaagcaggt cacaggtttc
     2281 ttgaaagaat tggaaaactt tgcccgcaag gaaaatattc ctattattcc ccatgaaacg
     2341 gttgcttatt tccgttttct tatggaaacc atgcagccta aaaatattct ggaaattggg
     2401 acggctatcg gtttttcagc tctcttgatg gctgaacatg cgccaaatgc taagattaca
     2461 actattgatc gtaatccaga aatgattggt tttgccaagg aaaattttgc ccagtttgac
     2521 agtcgcaagc aaatcactct cctagaggga gatgcggtgg atgtcttatc tacactgaca
     2581 gagtcttatg atttcgtctt tatggattct gccaagtcta aatacatcgt ctttctgcca
     2641 gaaatcctca aacatttgga agttggtggt gtggttgtct tggatgatat ttttcaaggt
     2701 ggtgatgttg ccaaggatat tatggaagtc cgtcgtggtc agcgaaccat ttatcgaggc
     2761 cttcaaaaat tatttgatgc aaccttagac aatccagaac tcaccgcaac attagtgcct
     2821 ttaggagatg gtattctcat gcttcgtaaa aatgtagcag atgttcaact gtctgaaagc
     2881 gaatgatttt cagaaaaatt taagaaaaaa tagtaaaata gatagagtaa cacttatctc
     2941 aaaggagtag acatgaagaa aaaattattg gcaggtgcca tcacactatt atcagtagca
     3001 actttagcag cttgttcgaa agggtcagaa ggggcagacc ttatcagcat gaaaggggat
     3061 gtcatcacag aacatcaatt ttatgagcaa gtgaaaaaca acccttcagc ccaacaagtc
     3121 ttgttaaata tgaccatcca aaaagttttt gaaaaacaat atggctcaga gcttgatgat
     3181 aaagaggttg atgatactat tgccgaagaa aaaaaacaat atggcgaaaa ctaccaacgt
     3241 gtcttgtcac aagcaggtat gactcttgaa acacgtaaag ctcaaattcg tacaagtaaa
     3301 ttagttgagt tggcagttaa gaaggtagca gaagctgaat tgacagatga agcctataag
     3361 aaagcctttg atgagtacac tccagatgta acggctcaaa tcatccgtct taataatgaa
     3421 gataaggcca aagaagttct cgaaaaagcc aaggcagaag gtgctgattt tgctcaatta
     3481 gccaaagata attcaactga tgaaaaaaca aaagaaaatg gtggagaaat tacctttgat
     3541 tctgcttcaa cagaagtacc tgagcaagtc aaaaaagccg ctttcgcttt agatgtggat
     3601 ggtgtttctg atgtgattac agcaactggc acacaagcct acagtagcca atattacatt
     3661 gtaaaactca ctaagaaaac agaaaaatca tctaatattg atgactacaa agaaaaatta
     3721 aaaactgtta tcttgactca aaaacaaaat gattcaacat ttgttcaaag cattatcgga
     3781 aaagaattgc aagcagccaa tatcaaggtt aaggaccaag ccttccaaaa tatctttacc
     3841 caatatatcg gtggtggaga ttcaagctca agcagtagta catcaaacga atagtccaaa
     3901 tcaatgagtc agggaaaaaa actcgacttc aggaaaaaat gaagcaaaca ttcccacaat
     3961 aaaacgcata gtacaaggtt tgtactgacc ccaaaagtta gacaattaat ttatctgaag
     4021 gatttagttc tgtattgcac agggctaagt ccttttagtt ttaccttaat tctcttattg
     4081 ttgtaataat caatatagtc tataatggct tgttccaatt gattaagtga tttaaatgtt
     4141 ttctcatagc cataaaacat ttcggatttt aaaatgccaa agaaagattc catcctaccg
     4201 ttgtcttggc tgtttccttt gcgtgacata gatgcttgaa
//
LOCUS       DAVBHS010000148         4223 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVBHS010000148 DAVBHS010000000
VERSION     DAVBHS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.148"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            170..1336
                     /locus_tag="VQ651_001807"
     CDS             170..1336
                     /locus_tag="VQ651_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET2754762.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVAKPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVVLGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            1452..2489
                     /locus_tag="VQ651_001808"
     CDS             1452..2489
                     /locus_tag="VQ651_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HET2754763.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGALLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            2697..3551
                     /locus_tag="VQ651_001809"
     CDS             2697..3551
                     /locus_tag="VQ651_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2754764.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREYYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            3701..3958
                     /locus_tag="VQ651_001810"
     CDS             3701..3958
                     /locus_tag="VQ651_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET2754765.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
BASE COUNT         1258 a          766 c          969 g         1230 t
ORIGIN      
        1 acctcaaagc agtgctttga gcagcctgcg gctagtttcc tacagatttt agttggaact
       61 cgattcaatt catgtgacaa cgtgaaaatc gttagagcat tttatataga atatacatgg
      121 gaatggagct tactcccatt cccatattta atagaaaaag aggaactcaa tgctacatta
      181 tacaaaagaa gacttgctcg aattgggtgc agaaatcact acgcgtgaaa tctaccaaca
      241 gcctgatgta tggagagaag cttttgaatt ttatcaagca aaacgtgaag aaattgcagc
      301 ctttctacaa gaaatcgctg ataaacatga ctatattaag gttatcttga caggtgctgg
      361 gacttctgct tatgtgggag ataccttgct accttacttt aaggaagtct atgacgaacg
      421 caaatggaat ttcaatgcta ttgcgacaac agatatcgtt gccaaaccag caacctattt
      481 gaaaaaagat gtggcaactg tccttgtgtc ttttgctcgt agtgggaact cgcctgaaag
      541 tgtggcgact gttgatttgg ccaaatcctt ggtggatgag ctttatcaag tgacgattac
      601 ttgtgcagca gatggtaaat tggctcttca agctcacggt gatgaccgta atctcttgct
      661 cttgcaacca gctgtctcta atgatgctgg atttgccatg acttctagct ttacgtctat
      721 gatgttgaca actctcttgg tctttgatcc tacagaattt gctgttaagt ctgaacgttt
      781 tgaagttgta tctagtcttg cccgtaaagt tttagacaag gcagaagatg tcaaagagct
      841 cgttgattta gactttaacc gtgtcatcta tctaggcgct ggtcctttct ttggacttgc
      901 tcatgaagct cagctcaaga ttttggaatt aactgctggt caagttgcga ccatgtatga
      961 aagcccagtt ggcttccgtc acggtccaaa atctcttatc aacgacaata cagttgtttt
     1021 ggtctttggt acaacgacgg actacactcg taagtacgac ttggacttgg ttcgtgaagt
     1081 tgctggtgac cagattgctc gtcgtgttgt gcttttgagt gatcaagctt ttggtcttga
     1141 aaatgtcaaa gaagtggtcc ttggttgtgg cggtgtcttg aatgatattt accgtgtctt
     1201 cccttacatc gtttatgccc aactctttgc cctattgact tcactcaagg tagaaaataa
     1261 accagataca ccgtctccta caggtacagt aaaccgtgta gtacaaggtg tcataattca
     1321 cgaatatcaa aagtaagaca gtgtttatga attcttgaca agaggatttg taaattatca
     1381 gataaaccat agattgtcag tacgctttct atggtttgtt tgcttgagag aaatagtaaa
     1441 aggagaacag aatgaaagca tacacagagc gtgtatttgg aaatgttgag ggtgaggatg
     1501 tcttggccta tcgatttgag acagacggtg gctaccaact tgaggttatg acttatggtg
     1561 cgactatctt gcgctatgtc gcacctgaca aggctggaaa ttttgccaat gttatcttgg
     1621 gatttgatga ctttgatagt tatgtaggca atagtcccaa gcatggagca agtgtaggtc
     1681 ctgtagcggg tcgtattgca ggtgcgacct ttgagctcaa tggtaagacc tatgaccttg
     1741 aggttaataa tgctagcaac tgtaatcaca gtggttcaac tggttgggat tccagcttgt
     1801 ttgaagttga agaagtaagc gatcatggct tgactctcta cacagagcgt acagatggga
     1861 caggagggtt ccctggaaat ctcaagattt ggatcagtta tcacttggaa gaaactggtg
     1921 cctatgaaat cagctacaag gtaacgaccg atcaggatac gctggtcaat ccaaccaacc
     1981 acagctattt caacttgtct ggtgatttca cgcagacgat tgaccgtcat gtcttccaac
     2041 taaacacaga gggcatttac tcaatcgctc ctgacggtgt tcctgccaaa actccagaag
     2101 ccaaccgtga tgtggtcaaa cacatctaca atggtgcctt gttgaaggat atctttgcag
     2161 aagaagatga gcaaatccag ctggcatcag gtttggatca tccatttgcc cttcctgcag
     2221 gccatgacaa tgctggattc ctttatgacc aaaattcagg tcgcttcctg cttttcaaga
     2281 cagaagctcc ttgctttgtg gtctacacag caaactttgt ggatgaaagt gtcatcatag
     2341 gaggtcagcc aatgctacag cacaatggga ttgctcttga agcgcaagct ttaccagatg
     2401 ccattcacag tgaccttaaa ggccaagtca ttcttaaagc tggtcaaacc ttcaccagta
     2461 agacacgtta tgaacttgtt gtgaaataaa aaaagctggt caaaaagtct ttaaaattaa
     2521 aataacgcat attgtcaggt gatgaaaagt ttgataatat gcgttttatt atgaaatgat
     2581 ttactttata ttatcctgaa atggtgttat ttttgagtta tctggcttga ctttcttgat
     2641 ggaaattata taatagttgt aacaatgata attacaacta gaaaagagga ccatttatga
     2701 cctatgaata caagagtcac atttatttgg cagagacagc tttaaatgta aaggatttgg
     2761 ccagtcaaac agccttttat cagcaagtta ttggtttaga aatcttatct caaactgaga
     2821 cagagtccat tctaggcctt ggtggaaaag tcttggtaca gctgattcaa gcacaagaga
     2881 gcggagaagt gagggaatat tatggtcttt accatttggc cattctcttg cccacacgca
     2941 aggctttagc tgatgtcttg aaacacctga cagatttaca aattcctctt gtcggcggtg
     3001 cagaccatgg ttacagtgaa gctatttact tggaagattt ggagggaaat ggcattgaac
     3061 tctatcgaga taagccagtt tccacatggg atattcgaga agatggacgc attatcggag
     3121 tgacagaggc tcttgcggcg caggacattt atgagttggg ggaaagagta gagcccttta
     3181 tcctggcaga aggtacgaga atggggcata ttcatctttc cgtcaaggat agtcgaaagt
     3241 ccagccagtt ttatcaaaag gtgttagggc tagaggataa attcagtgtg cctagcgcta
     3301 gttggattgc ggctggagat taccatcatc atttagcagt taacgaatgg ggaggaaaag
     3361 gtctggcttc gcgtaagcaa ggcttaccag gtttagccta ctatgtcatc gaagtcgcac
     3421 acaaaaaaga actattaacg attgcccaac gagcacaaga agttgacgca ccaatcaaat
     3481 ggatgacatc gagccaattg gaaatcacag actcagatgg catcgtgacc cgtattcgtt
     3541 tagcaaggta aaattaggaa tttttaataa gttataagta ttgctagttt gctttttgaa
     3601 cggtctttgg taacagaact gcgtgagctg gatgggcgag aattttgaca aagaagctga
     3661 ttagagatat aatgaagaaa aatcgagcaa ggaaaaagaa atgacaaatt caaagtatat
     3721 tacttgtttg aaacgttcag agggccagtt gtgtgggatt caaaagatga ttgaaggaga
     3781 tcgtgactgt gctgatattg tgacgcaact gacagcagtg agatctagtg tagagcgcgt
     3841 gattgagatg ataattaccg aaaatcttac tgaatgtatc aatcagccac tagatgattc
     3901 tgaagctcaa aaggaacgct tagaaaaggc tatccgatac ttgattaaac ggaaataaat
     3961 gaatgttttg agacgatatg tgcaaaggtc tattaactat tagaatagtt taatgggagt
     4021 caaaagaagt tgtaaaaaat ctttaaaatt aaaaaaacgc atagtatcag gtattgaaat
     4081 gtactgaccc caaaaagtta gattttttct gtctaacttt tggggtgcag ttcataagaa
     4141 ccttggtaat atgcgtttta ttatggggag atttatactt ttcgaaaatc tcttcaaacc
     4201 aggtcagctt caccttgccg tag
//
LOCUS       DAVBHS010000149         4195 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVBHS010000149 DAVBHS010000000
VERSION     DAVBHS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.149"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(173..838)
                     /locus_tag="VQ651_001811"
     CDS             complement(173..838)
                     /locus_tag="VQ651_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET2754766.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            complement(852..2231)
                     /locus_tag="VQ651_001812"
     CDS             complement(852..2231)
                     /locus_tag="VQ651_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET2754767.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            complement(2844..3608)
                     /locus_tag="VQ651_001813"
     CDS             complement(2844..3608)
                     /locus_tag="VQ651_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HET2754768.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLDEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            3678..3839
                     /locus_tag="VQ651_001814"
     CDS             3678..3839
                     /locus_tag="VQ651_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HET2754769.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            3829..4125
                     /locus_tag="VQ651_001815"
     CDS             3829..4125
                     /locus_tag="VQ651_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2754770.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLISQGLTVTNVEGGMNAWPGQVE"
BASE COUNT         1354 a          986 c          793 g         1062 t
ORIGIN      
        1 cccgcagcta gtttcctagt ttgatctttg attttcattg agtattactc tctcttgtca
       61 cttccttcta ttttaccata aagtccagcc tttgaagaac ttttactaga agacaagggg
      121 cttctgtctc tatttgccat cttgggcatc aaaaaagagg ggtcatccct ctttacgaat
      181 tcaatgctac tagggtatcc aaatactggt tgttgatgac tgccaaaata taggtatctg
      241 ctttcaagag gtcatctggt ccaaattcaa catccaatgg ggaattttcc tgctctcgga
      301 aacccaaaat attcagattg tatttgccac ggaggtctaa tttactcaga ctttgacctg
      361 cccaagactg aggaattttc atctccacga tagacacatt tttatccaac tgaaagacat
      421 caacactatt atggaaaaga atggtctgtg ctagagactg ccccatttca tactctggcg
      481 agataaccga gtcagctcca atcttttcta gcactttctt agcggtctga cttttgacct
      541 tagcaataac agtcggtacc cccaaactct tacagtgcat aaccgcaagc acactcgact
      601 ccagattttc acctgtcgcg actacaacgg tatcgcaggt atcaatccct gctgatctca
      661 ataattcttc atctgtgatg tcaccaatca ctccacgcgc caaaactggc tcaaactgat
      721 tgatgcgctc tgcgtggtca tcaatagcga taatattcat atcctgcttg gctagggcag
      781 ctaggacact gctcccaaaa attcccaagc ccaaaattcc aatcgtacga tctgacatgc
      841 tctttccttt cttaaccaat actaatatct gctttcatat agtgaatcat atctttcttt
      901 tctggatggt aatctgccaa gctaacaaac aaggtcaagg gaccaattcg tcccataaac
      961 ataagtggca tgataacact gagagccaat ttcccaaggt caggagtcag atttgccgtt
     1021 acaccaactg tactaagagc tgaaatggtt tcaaatatga ggtggataaa gggaggattg
     1081 cctttggctg ttatccctag cagaatcaat cctatcaaga agctcatcaa aaagataata
     1141 aagacactaa aggatttttg aaccgttcgc ggcgcaatcg ttcgtctcgc aacattggca
     1201 tgaggcaagc ctagaagctc acttcgtgca aagaccaaga ggacaaaaaa tgtcgtaatc
     1261 ttgagtcccc cagctgttcc tccaggtgcc ccacctagaa acatctgtaa gatataaatc
     1321 aaaagagtca caggatgagc ctgagtataa tctatcgtag aaaagccagc tgttcgcatc
     1381 gtcactgttt gaaaaaagct aactaaaacc ttatcggcaa cagggagatt gccaatcgtt
     1441 ccagcattgt tccactcaag aaagagagta gttgccgttc caaataacaa caaacctata
     1501 gtcaacaata gtacaagctt cgtatgaaag tgcagacgtc cttttttctt tcttcctaca
     1561 tgaccagcca aatcaaacca gaccataaaa ccaaggccgc ctgtaataat caagcctgca
     1621 atcaccagat tgaccagtaa atcggtctga aaagcaaata aactggtgct ccctaaatta
     1681 tcaaaaccgg cattacagaa ggctgagatc gctagaaaaa tggaactaaa aagaccacgt
     1741 ccccagccaa gttgaggaat aaggcgaaaa ctaagcaaaa tagctcccaa gctctcaacc
     1801 aaaaaggtcg tgagaaaaat agaatagaca aactttctca aagatcgagt ttctccataa
     1861 ctaaaactat cctgaatagt tgcacggcta cgaagactaa gcttttgctt gctctggata
     1921 tagaaaaccc caataaaggt catgagccct agaccaccga tctgaatcaa gagcaaacag
     1981 attatttggc cccatatatt ataggtgtga gctactggaa gggttgagag acctgtcaca
     2041 cagactgcag agacagcagt gaaaagatga tcaaaataag tcgctcgtga gctttcaact
     2101 tggacaaagg gcaagctcaa aagaagagag cctagtaaaa tgaccaaggc aaaactcaaa
     2161 aaaatacgac gggctggcga taaacgagat aagcctagct ctattttttc cttagataat
     2221 ttgaataaca tgtttccatt gtaccataaa aagttcgaaa tgacttagat aggataaggc
     2281 aaaatctact aacaccaagc ctaaaaaatg ccaccttttt attgatagct taggcaagaa
     2341 caatacttga tagatgtgac tataggtcaa aaaagcaaga caggagcttt taacgccagc
     2401 tactctatat gcttttcagg agaaaatttc tctttttgat tataggcaat gagaaccaag
     2461 gcatcgatta gaaagctagt cacagattgc tcaaaacact gttttgaggt tgcaaataga
     2521 actgacgaag tcagctcaaa acaccgtttt gaggttgccg gataagactg acgaagtcag
     2581 taacatatac ctacggcaag gtgaagctga cctggtttga aaagattttc gaagagtatc
     2641 atttcatctc actctaaatc ttaccaacat aaaacgcata gtatcaaggt tttcatcacc
     2701 tgatatgata cgtttttctg attttaaaga tttttcccaa cctcaacatg cttaggacaa
     2761 gaggaaggtg acctcgttta aacatatttt tgaaaagtat gagctagaca ttttatgata
     2821 aaacaaatcc tttgccagta caactaaagg tggtgaacac ttgctaaact cagttctagc
     2881 gccttattaa aagcttccga gccccagtca cgactgtcgt actggtccaa gtccgctaga
     2941 gaatctgctg tgaacaacaa ttcaccccag agaaccccac gcaattgagc tactgccgca
     3001 agagcagaac actccatctc cacaacagca cagccttctt ccttacgata agccaccttt
     3061 tcagccgttt ctcggtaaaa accgtctgtc gtccaggtca tgacttcttc ataaggaatc
     3121 cctctgtctt ccaaaacttc ctcaatagca gcaatagcct ctggctgcat ttccatataa
     3181 cgagaaggtg ccacatagtg gtaactggct ccttcatctc gcagagcgcg aacagggact
     3241 agaaaggcat tttcctctat atcagctaga acaccacagg tcccagtaga gataatctgc
     3301 tccacaccat agccaatcaa ccaatccata aactgggctg ctggagcgga gccaacagga
     3361 gcctgagcca gacagacctc ctcacccttg tagttgatga catagactgg ataggtcttg
     3421 gtagcagaaa cgaattcgcc gacacaatcc gcccctactt cccttgcata gcggtcaatc
     3481 tcctcatcta aaaatgcata aacacacttc tttggcaact gcaagtccag cccctcgtga
     3541 ttgggcatga taaccgcctg aggattgtca tcaaactcta aaataggaat cgcatgtttc
     3601 tgaatcatag cccacctcct ctaattttgt actattgtat caaaaactga caaagtgtca
     3661 aggaggaagc tgatgaactg gacaaaaaac aagcctacat cgtcagctgc cacagtggtt
     3721 tgcgcagcta tatcgcagag cctatcctca agcaagcagg atttaccgcc caaaaccttg
     3781 acggcgctta ttcactatac aaaatggtta acccagaagg agtagaatat ggaaactagt
     3841 atcagcatgg ctgactttta tggaaaatac caaaatgaaa atctagaact tatcgatgtc
     3901 cgtgaagcac atgaattcca agcaggacat gcaccaggtg ccaaaaatct tccattaagt
     3961 accttagagc aaggctacaa agaactcaaa cctgaccatg aatactatgt tatttgtcaa
     4021 ggaggagtcc gctctgcatc tacctgccaa tttctcatct cccaaggcct caccgttacc
     4081 aacgtagaag gtggtatgaa tgcttggcct ggtcaagtag aataatcaag aggaactggg
     4141 caaaaactca atttcatgag aagatgaagt aatactcaat gaaaattaaa gtgca
//
LOCUS       DAVBHS010000150         4187 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVBHS010000150 DAVBHS010000000
VERSION     DAVBHS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.150"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            80..724
                     /locus_tag="VQ651_001816"
     CDS             80..724
                     /locus_tag="VQ651_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HET2754771.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNEEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(766..2754)
                     /locus_tag="VQ651_001817"
     CDS             complement(766..2754)
                     /locus_tag="VQ651_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2754772.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNEGVKEQKALTLNDHQFSVKEEFTKDFIVNHVPNQF
                     NILTADYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVNASEAEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFLHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(2756..3514)
                     /locus_tag="VQ651_001818"
     CDS             complement(2756..3514)
                     /locus_tag="VQ651_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754773.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            3942..4133
                     /locus_tag="VQ651_001819"
     CDS             3942..4133
                     /locus_tag="VQ651_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754774.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
BASE COUNT         1276 a          826 c          814 g         1271 t
ORIGIN      
        1 tggatggcta acttgaactt gaaatttagc gtcagaaaca aatcacgaaa ttgattcaaa
       61 ttttgcaggt cgtttaaata tcctgcgtgc gggtgttctt gatgctaacg atggaattat
      121 ttccattgct ggtgtggtta tcggggttgc cagtgccacg accaatatct ggattatctt
      181 tttatcagga tttgcggcta tcttagctgg tgccttttca atggctggtg gagaatatgt
      241 atccgtttca actccaaaag ataccgagga agctgccgtt tcgcgagaaa aactcttgct
      301 agaccaagat agggaactag ccaaaaaatc cctctatgct gcttatatcc aaaatgaaga
      361 attcaaaact tctgcccaac tcttgactaa taagatcttt cttaaaaatc cactcaaggc
      421 tctggtagag gaaaaatatg ggattgagta tgaagaattt accaatcctt ggcacgctgc
      481 catttctagc ttcgttgcct ttttccttag aagtttgcct ccaatgctgt cagtgaccat
      541 ttttccaagt gattaccgca tccctgctac cgtccttatt gtcggtgtgg cccttcttct
      601 cactggttac acaagtgcta gacttggaaa agccccaacc aaaacagcta tgattcggaa
      661 ccttgctatt ggtctcttga ccatgggagt taccttcctg ctcgaacaac ttttcagcat
      721 ttagaataca agaaatacct cgattttgaa gtcgaggtat cttttttaca tttgcacaat
      781 cttgcgataa cttcttgaag taatcatgaa aatcagcaca taggcgatga ggaagatagc
      841 gcagatagac aaggtcacaa tcaacatcat agtcgtatcc agtacaccaa tcacttttaa
      901 aatcagacta agcatatggt aggcaaaggc tagatgtagg aaggcaaaga gcaaaggaag
      961 gaagaataca gttaaaacct gtttgttgat ggtttgcttg atttgctttt gatctaaacc
     1021 gactttctgc aagataataa agcgctcacg gtcttcatat ccttcagaaa tttgtttgta
     1081 gtagatgacc agaacggttc cgaccataaa gataatggat aagaaaatac cgataaagaa
     1141 gacaccacca aagagggcac tcatctgagc actagcatct gctagagtgc taccatacac
     1201 atagttacct tcagtgttta attgagcatt aaacttttgt aagtattttt catactcctc
     1261 agcgaccttg agttgttctg cttcactggc atttacattc ataccaccgt aaaactgatt
     1321 atagatagcc gaatctggga attgatccaa aaaggcttgt aaatcaggta caacaaggta
     1381 attgtaatca gcagtcaaaa tattaaactg atttggaaca tggttgacaa taaaatcttt
     1441 agtaaattct tcttttacag aaaattgatg atcatttaga gttagagctt tctgttcttt
     1501 aactccctca ttctttgcaa agagtccgac ctcatttcct gatagagaca gtttttgacc
     1561 agtcatattt tcataatctt tttggtcaaa taccatgaaa actgtttttg gttggacacg
     1621 gttttgtcct ttttcaaaaa tagttaactt agttccttct tgatttgcaa taccaaagtt
     1681 actgtaacga agtacttctt tctctttgac actataacct ttgtcacttg caaactggct
     1741 caagagtttg tccaagtctt ctttttcaac attttgccct gaaaccccaa aatcatgagg
     1801 atttagaact tttttaaagc tttctgagga attgaaaatg cttgtcgctg ctgacatggt
     1861 taccaaaacc attgttgaca agatagcgat ggtggctagt ccaactgcat ttttcttcat
     1921 acggaaaatc aagttagaaa cagatatgag attatttggt tggtaatagt atttcttatt
     1981 tttctttaag atttggagga aaacggtaat ccctgcatta aacaagagat aagtcccaaa
     2041 gataaccagt aaaacagcta tgaagaaggt tgttaaggct gtaagaggat cttttaccgt
     2101 aagggcaaga taatagccaa ttcctaaact tatggaacca agaatggttt ggagaggaag
     2161 gaagcgacct tttttctctc cactagcttt ctcacgagag agctggaggg cattcatacg
     2221 ggcgattcga agggcattca ggaacatgag gcctaggaaa atcaaaccga agacaacaag
     2281 cactgtaatg acaactttcg tctggaaggt agcaaccagc tcaaccttca atttcattag
     2341 tttgagcagg aaagcgaaaa ttaacttgtc aaacaaggct ccaataccga tacccgctcc
     2401 aacagttaga atcccaaata ccactaactc cttaaaggtc atactgataa gatgacgctt
     2461 ctccaagccc aacatgccat aaattcctag ttccttggaa cggttcttca tgacaaaact
     2521 attggcatag agaacgataa tggctgacgc aagggtgacg acaaacatac caaatccaag
     2581 tgtagcctga atggttgttc ctccacgaat ttcagcaatc ttgggattga aggttagaga
     2641 gtaaaagaga taggtgacag tgactgccaa gagaacagcc agcgcaaaag gatagtagag
     2701 tttgcggttt ttaatcaagt tcgataccgc taacttattg gttaatcgaa acatactaat
     2761 tcacctcgct tgccatgaca gtcaaggtat cagagatttc ttggaacatc tgacgttctg
     2821 tcttctctcc acggtagatt tgattgtaaa gaatgccgtc tttgataaag agtacacgtt
     2881 tagctctgct agctgctgct gttgagtggg ttaccatgag aatggtttgg ccgcgctcat
     2941 tgatttcatc aaagacatca agtaaggctg cagatgactt ggagtcaaga gctcctgtcg
     3001 gttcgtccgc aaggagaatt tcaggttcag tgatgatggc gcgtgctact gctacacgct
     3061 gtttctggcc accagaaatc tcgtaagggt acttctcttg taattggttt attcccagat
     3121 tctcagctgt caccaccaat ttcttcatca tctctgtaat aggtcttctt gacaagacaa
     3181 gcggaagcaa gatattgtcc tttacagata gagtatctag caagttaaag tcttggaaga
     3241 caaagcccaa cttttcacga cggaagctag aggcctgtga atttttaatg gttgcggtgt
     3301 cagttccatt caagtaaacc tgaccacgac ttggtttatc cagcatagct agaatattga
     3361 gaagagttga tttaccagaa ccagactcac ccatgatggc aacgtagtca cccttttcga
     3421 cggtaaagtg aatatccttg agggcttcta cttggttgcc ctgaaaacgt gttttataaa
     3481 ttttttgaac gtgttttaca tctaaaagtg tcatgagaat ctcctttctt aatatcccat
     3541 caaatgaaat gtagcttgca tcatagtgca tcccagctga acgcgctcaa tttctttgtg
     3601 acttggtttt ctagttttct tatgtaagat ttgtttcatg atgttactcc tttgttcttt
     3661 atgagtctag tttacatcaa aaaaagaccg cttgccataa cctaacctta caaaagagac
     3721 tttaatctta catttttgta agattgggtg aaatataagc aaaattactt agaaaatgat
     3781 ttgatttcct atttttaaag ttgtaaaata taagtatgaa aaggcttact ttggaaagga
     3841 ggaagcggga actatcattt gagtaagctt acctagataa cataccattc aatttaacac
     3901 ttgcaagtcg ttcaacctat aaggaaagaa ggtgcgcttt aatgaaacac aaagaacata
     3961 tactcatcgg acttctctat ctgctctctc cattcatcgg tcagctctta gttgaacaca
     4021 cacactttat cggtacagaa tttaccggca ctgcttatgt tatttgctgg ttatcagttg
     4081 ttatcagcat ccaccatttt tccaaaaatg tattgtctca gcagcaaaaa tagattaaaa
     4141 aactgagttt aaattactgc ttttataata aaatgaaata agaacag
//
LOCUS       DAVBHS010000151         4139 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVBHS010000151 DAVBHS010000000
VERSION     DAVBHS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.151"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..160)
                     /locus_tag="VQ651_001820"
     CDS             complement(<1..160)
                     /locus_tag="VQ651_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HET2754775.1"
                     /translation="MNIKKRVLSAGLTFASVLLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYTD"
     gene            complement(318..1925)
                     /locus_tag="VQ651_001821"
     CDS             complement(318..1925)
                     /locus_tag="VQ651_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HET2754776.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2078..3562
                     /locus_tag="VQ651_001822"
     CDS             2078..3562
                     /locus_tag="VQ651_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HET2754777.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFLNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGISPADRKVAVVARQKAEETGGPALALELPNGDIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            3657..4139
                     /locus_tag="VQ651_001823"
     CDS             3657..4139
                     /locus_tag="VQ651_001823"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HET2754778.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
BASE COUNT         1201 a          951 c          792 g         1195 t
ORIGIN      
        1 tatcagtgta atagtctaat agatagttaa atgtagttgg atttccacta aaggttgatg
       61 agtaagtttt tgtatctgaa cctgattggc cgcaagcagc taaaagcaaa acagatgcaa
      121 aagtcaggcc tgcactaagg acacgcttct ttatgttcat cttctttctc cttaatagtg
      181 ggaatttgta aagttaattg aatttcgaga atgaaagttt ataaaccttg gttataaaaa
      241 acaaaggatt tctgctttta tacagtcctc ccttgttttt atatgatttc aattttaaat
      301 ttttctgcaa aaaatattta tagtaattcc acacagaaag catcccatgg aactaagatt
      361 tgtttttcaa agacttcttg agctagggtg ttttcaatca agacagattt gacttttcct
      421 tctactgtca agtcttgctc ttcattggac aagttagcca caactaggaa gcgacggtcg
      481 ccatccttac gtatataagc aaagacctta tcagccgtat caagcaattc aaagtcagct
      541 cgaactagcc agctattctc cttgcgaatt tggaccagtt tctgataggt atagaaaata
      601 gaatctggat ttgccagcgc ttcttggaca ttgatcatct cgtaatttgg attaaccgcc
      661 aaccaaggtt gacctgttga gaaaccagcg tttttgctct cgtcccattg cataggggta
      721 cgggcattgt cacgtccaat aacacggata ctgtccatga tttcttcaat cggaacacct
      781 ttttcaagag cctcacgcgc atagttgaga gattcaatat cttctacttg atccagtgtt
      841 tcaaacggat agttggtcat cccaatctcc tcaccttggt agatataagg agttcctctc
      901 ataagatgaa gcaagattgc aaaggctttg gcagattttt cgcggtattc ttggtcattt
      961 ccccagattg agacaatacg agggaggtca tggttgttcc agaagaggga attccagccg
     1021 tcctcaactc ctaactctgt ctgccatttg ttgaagattt cttttaactt agcgatattc
     1081 agctcttttt gatagtgcca tttaggctga ccttcctgat actgaagacc gatatgttca
     1141 aactggaaga ccatagacaa ttcttgcccc tttggatcag agtagagctt ggcaatctct
     1201 ggcgttgctc cccaagtctc ccctactgtc aagagatcct tatctccaaa ggtcgcctga
     1261 ttcatttcct tgagataggg atggagcata ggaccattat tgactacctt ctcgtcagga
     1321 attttgccaa tcatgtcaat aacatccata cggaaaccac caataccctt atcaatccag
     1381 aagttcatca tctcataaat tttctggcga agtttttcat tttcccagtt gagatccggc
     1441 tgtttcttgc tgaaaaagtg gagatagtat tgacctgact tttcatcgta ttcccaagca
     1501 gacccactaa agatagaatc taaatcgtta ggctcatccc gccagatata gtagtctcgc
     1561 tcagggctat taggattttc acaggcctct acaaaccagg catgctcatc cgaggtatga
     1621 ttgaccacca agtccatgat gatacggata tcacgcttct tagcttctgc aatcagctga
     1681 tccatgtcct ccatggttcc aaaaatagcc gcaatcgctt gataatcagc aatatcgtag
     1741 ccattatcat ccatagggct gtcataaacg ggagaaagcc aaattgctgt gattcctagc
     1801 ttggctagat agtccaactt actggtaatt cctggcaaat cgccaattcc atctccatta
     1861 ctatccataa aactctttgg atagacttga tagactacgg cattatgcca ccatttttct
     1921 tgcatctgtt tacctcatta ttttcataat ctatagtcta tgatagcact ttttaaaatt
     1981 ttgtgcaagc gtttgcttaa tctttttaat ttctttttta gctccgtagt ttcctaactt
     2041 ccaatttttt attataatag agtccagagg taaaaaaatg aaaaaacaag cttttagttc
     2101 tgaacagtat ttgaatttac aacgcgacca tattttggag cgcattaacc aatttgacgg
     2161 caagctctac ttagagtttg gcggtaaaat gttagaagat ttccacgctg ctcgtgtcct
     2221 tcctggttat gaacctgaca acaaaatcaa gctcttgcaa gaattgaaag agcaggttga
     2281 ggttgtgatt gccattaatg ctagcaacat tgaacattcc aaagcacgtg gcgacttggg
     2341 catttcttat gaccaagaag ttcttcgttt gattgacaaa ttcaatgaat tagggatttt
     2401 tgttggttcc gttgtcatta cacaatacgc tggccaaccc gctgcagatg ccttcctcaa
     2461 ccaacttgag aaaaacggaa ttgattctta tcttcattat ccaatcaaag gatatccgac
     2521 ggatatggat cacatcattt ccccagaagg catgggcaaa aacgactaca tcaaaaccag
     2581 tcgcaacttg attgtcgtaa ccgctcctgg acccggttct ggaaaattgg caacgtgtat
     2641 gtccaatatg taccacgacc aaatcaatgg catcaagtct ggctacgcta aatttgaaac
     2701 cttccctgtt tggaatcttc cccttcatca tccagttaac ttggcttatg aagctgccac
     2761 agctgacctt gatgatgtca acatgattga ccccttccat cttcaaacct atggagaaac
     2821 cactgtcaac tacaaccgtg atatcgaaat cttcccagtg ctcaagcgca tgttggaacg
     2881 tattctcgga aaatcaccat acgcttcacc gacagatatg ggtgtcaaca tggttggttt
     2941 cgctattaca gatgacgagg ctgctgtcga agcttctaaa caagaaatca tccgccgtta
     3001 ctatcaaaca gttcttgact tcaaagctga aaaagttggc gaagctgccg tcaagaaaat
     3061 tgagttgctc atgaacgacc tcggtatctc acctgcagac cgtaaggttg ctgtcgttgc
     3121 gcgccaaaaa gcagaagaaa ctggcggacc agccctagct cttgaattac caaatggtga
     3181 cattgtcact ggtaaaaact cagaactctt tggccctaca gccgctgcct tgatcaacgc
     3241 catcaaaaaa tcagctgaca tcgctaaaga agtaaaacta atcgagcctg aagttgttaa
     3301 gccaatccaa ggacttaaaa tcgatcatct cggtagccgc aatccacgcc ttcattcaaa
     3361 tgaaatcctg attgcacttg ctatcacagc tacagaaaat cctgatgctg cccgcgctat
     3421 ggaagaactc ggcaacctca aaggaagcga agcccactca accatcatct tgactgatga
     3481 agacaagaat gtccttcgta aactgggtat caacgtaacc tttgacccat actaccaata
     3541 cgaccgctta tatcgtaagt aaagatttca acctctccag cctcggagag gttttctctc
     3601 tgtctcagga gcaagcttta tgatataatg agagaagaaa actgaaagga tttaccatgt
     3661 caaaagaagt tattgtcgaa agttttgaac ttgaccacac cattgtcaaa gcaccctatg
     3721 ttcgcttgat tggggaagaa acaggaccaa aaggagacat catctccaat tatgatattc
     3781 gcttggtgca accaaacgaa gactctatcc ctactgccgg ccttcacact atcgagcacc
     3841 tcttagccaa actcatccgt acccgcattg acggcatgat tgactgttca ccatttggtt
     3901 gccgcacagg cttccacatg attatgtggg gacgtcacac cagtgctaaa atcgcagctg
     3961 ttatcaagga ttcgctcaag gaaatcgctg aaactactac ttgggaagat gtcccaggga
     4021 caaccatcga atcttgcgga aactacaagg accacagcct cttctctgct aaagaatggg
     4081 caaaactcat cttggaacaa gggatttcag atgatgcctt tgaacgtcat gtgatttaa
//
LOCUS       DAVBHS010000152         4116 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVBHS010000152 DAVBHS010000000
VERSION     DAVBHS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.152"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..776
                     /locus_tag="VQ651_001824"
     CDS             <1..776
                     /locus_tag="VQ651_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754779.1"
                     /translation="DYSIVEEKLDWQSKLSKIYPNVEFIKIEKLKLILLCKYRNQIID
                     LEDDKKRLLLLKLLYLTSKQFCYDEFKGAKFGCMSFFSHYQGYMSRLDSLDSDKFVSV
                     KQRIVSSISKLVEQYSDHIINLDRTLVDSLSDTLENQLFDDWDYILSEITTYSELLAK
                     SGLLYNSLDYKIDNVKKEVAKLSDFHQSLFKIEGFSDFFDSWQFQRYRSLVNQVYEIF
                     SMLQVSSVERTVLCGALSTIVFSKSNFNYQAFFEYLKDT"
     gene            805..1713
                     /locus_tag="VQ651_001825"
     CDS             805..1713
                     /locus_tag="VQ651_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754780.1"
                     /translation="MDYVDVILDEYGSVFLIKDHIKLKINNLELYRYFSSFGMSHLQF
                     RKEELPGKLKQFDFEKNKSDSYFSKKSSLIDFIKRNYFYDIKLYSDNPKNPIESWKNK
                     TEKIKIAKSTEILWSEVANFSSISKSPYYCLLYNLKNTENYILIISKTYFSAIQKMVS
                     YNLNNYFELEDVCCKSIVSKRELIENSININKSKMKLLNVCEGSKGYTRRVFKYNNIF
                     VGQLEFHSNIIFSEIALNELSIVKLLDSVESVLSEQLFGKLNWDNLFVVLSDDFNDYL
                     DINESQKYYSNGSLRGLELGIFMGGV"
     gene            1715..3613
                     /locus_tag="VQ651_001826"
     CDS             1715..3613
                     /locus_tag="VQ651_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HET2754781.1"
                     /translation="MRIIEIVDVEDQLLQLLSAHTNIDIRAQELSTLDPDSKVDLIIY
                     LKSRRDIENINNIIIKSPNRNIIVIISAFDIIYALYFPKSTFRPCLNCLLLKLKDNNP
                     EHNLLLFLKYGTLPSKHFNLDNRLKSNLILFNEKLDPKVEEYSIEGVNTTNFTFHFSC
                     SCENSGYDMSERMADILLGEGKKLSPKNYRLKKSEHLFDFTRYLDRNFGFFHHSYDEK
                     ISEYAPLHAVEINVDAGYESGWGRTFMTEAATKSAFLESLERYCNSRNRRYKKLIRGC
                     FSDLSKKYNTLNPVDFCLSNKEYRDSSYKFHDFDSEKNYDWIFGKNITTGKICLLPSQ
                     LVFYKDFSKSEENERFVYETSNGCALGSSIEEAIFYGILEIIERDNFLLTWYLKEEVK
                     QLSWESLPSRLRIINERLKEDNCELKLFNITKEVGIPVIWALYINHNENASIRSFSAA
                     GCHINPLSAIESAIFEVITSVPTFERLIEVRNKESFIEEIQNNFSLIRDIDDHILYHA
                     HADYKNKMPFLFKNNYSEYQNEFDENRIFNTYSHATLTSDLQALIKIIEKKFGDIYVV
                     NISDKVSEKFGLYCVKVLIPGLLNMSFGHSNRRVNIERIKNYVDYRGGNVNSITLNSI
                     NHLPHPFP"
     gene            3637..>4116
                     /locus_tag="VQ651_001827"
     CDS             3637..>4116
                     /locus_tag="VQ651_001827"
                     /inference="COORDINATES: protein motif:HMM:NF025394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HET2754782.1"
                     /translation="MKRDWKSKHLFYHDVQQFDKLVIYLSKFMDKLVNQNQIEKYFYI
                     RYWEGGPHIRLRYLVRDTQNFETIDSMINKYAYKFWENNPPMKLLNKTDYKKIYNESI
                     DNNGKMDRKLRKNATIENIAYIPEIERYGGEEALIVSEDLFCICSQVSTALLNVVDNY
                     "
BASE COUNT         1539 a          443 c          712 g         1422 t
ORIGIN      
        1 cagattactc aatagtggaa gaaaaattag attggcaaag taaattatct aaaatttatc
       61 cgaatgttga atttataaaa attgaaaaat taaaattgat tttgttgtgt aaatatagaa
      121 atcaaattat tgacttagag gatgataaaa aaagattgtt actattgaaa ttattatatt
      181 tgacttcaaa gcaattttgc tatgatgaat ttaaaggagc gaaatttgga tgtatgtcat
      241 ttttttcaca ttatcaaggt tatatgagca gattagattc actcgactca gataaatttg
      301 tttcggttaa acagaggatt gtttcctcta tttctaaatt agttgagcaa tattcagatc
      361 atattataaa tctggataga actttagttg attctctaag tgataccctt gagaatcagc
      421 tatttgatga ttgggattat atattaagcg aaataactac atatagcgaa ttattagcaa
      481 aatcaggatt gttatataat tcgctagact ataagattga taatgtaaaa aaagaagtgg
      541 ccaagctaag tgactttcac caatctttat ttaaaattga aggattttca gatttttttg
      601 atagttggca atttcaaaga tataggtctc tcgtgaacca agtttatgaa attttttcaa
      661 tgttacaagt aagtagtgta gaaagaactg tgttgtgcgg agctctaagt actattgtct
      721 ttagtaagtc aaattttaac tatcaagctt tttttgagta tctaaaagat acgtagaatt
      781 cgattttaaa ggaggctgaa atatatggat tatgtagatg taatattgga tgagtatggg
      841 agtgtttttc tgataaagga tcacataaaa ctaaaaatta ataatctaga actatatcgg
      901 tattttagta gctttggaat gagtcatctt caatttagaa aagaagagct tccaggaaaa
      961 ttaaagcagt ttgattttga aaaaaataaa agtgacagtt atttttctaa gaaaagtagt
     1021 ctgattgatt ttattaagag aaattatttt tatgatatta agttatattc tgataatccg
     1081 aaaaatccga tagagtcttg gaaaaataag acagaaaaaa ttaagattgc taaaagtaca
     1141 gaaattcttt ggagtgaagt agcaaatttt tcgtcaattt ctaaatctcc ttactattgc
     1201 ttgttatata atttaaagaa tactgaaaat tacatattga taatcagtaa gacatatttt
     1261 tcagccatcc aaaaaatggt ttcgtataat ctaaataatt attttgaatt agaagatgtt
     1321 tgttgtaaaa gcatagtgag caagcgagaa ttgatagaaa attcaattaa tattaacaaa
     1381 agcaaaatga aactgctaaa tgtttgcgaa ggtagtaaag gttacacaag aagagtattt
     1441 aagtacaata atatttttgt gggacaattg gaatttcact ctaacataat cttttcagaa
     1501 attgctttaa atgaattaag tattgttaag cttttagatt ctgttgaaag tgttttatct
     1561 gagcaactat ttggaaaatt aaattgggat aatttatttg ttgttttatc agatgatttt
     1621 aatgattatc tggatataaa tgaatctcaa aaatactact caaatggttc tttgagagga
     1681 ctggagcttg gaatctttat gggaggggtt taaaatgaga ataattgaaa ttgttgatgt
     1741 tgaggaccaa cttttacaac ttttaagtgc acataccaat attgatatta gagcacaaga
     1801 gttatcaaca cttgacccag attcaaaagt tgatttgatc atatacttga aatctcgacg
     1861 tgatattgaa aacataaata atattattat taaatcacct aacaggaata tcattgttat
     1921 aataagtgct tttgatatta tatatgcatt gtattttcca aaatcaacat ttagaccttg
     1981 tttgaactgt ctattattaa aacttaaaga taacaaccct gagcataatc tattattatt
     2041 cctgaaatac ggaacattac ctagtaaaca ttttaatctt gataataggt taaaatcaaa
     2101 tttaatacta tttaatgaga agttagaccc taaggtagag gagtatagta ttgagggggt
     2161 aaatactaca aattttactt tccattttag ttgtagctgt gaaaatagtg gttacgatat
     2221 gtcggaaaga atggcagata ttctacttgg tgaaggaaaa aaattatctc ctaaaaatta
     2281 tcgtttaaaa aagagtgagc atctattcga ttttactaga tatctagata gaaattttgg
     2341 attttttcat cattcatatg atgaaaaaat ctccgaatat gcgcctctcc atgcagtaga
     2401 gattaatgtt gatgctggtt acgagtcagg ctggggaagg acttttatga cagaggctgc
     2461 aacaaaatct gcttttttag agtcattaga gagatactgc aactctagaa atagacgcta
     2521 taaaaaatta attagagggt gtttttctga tttatctaaa aagtacaata cattaaatcc
     2581 tgttgatttt tgtctttcta ataaagaata tagagatagt tcctataaat ttcatgattt
     2641 tgattctgag aaaaattatg attggatatt tggtaaaaat ataactaccg gaaaaatttg
     2701 cttattgccc tcgcaactag tattttataa agactttagt aaaagcgaag aaaatgaaag
     2761 gtttgtttat gaaacatcaa atggatgtgc tttggggagt agtattgagg aagctatttt
     2821 ttatgggatt ttagagatta ttgaaagaga taatttttta cttacatggt atttaaaaga
     2881 agaagtaaaa caattatcat gggaatcttt accatctcgc ctacgtatta ttaatgaaag
     2941 actaaaagaa gataactgtg agctcaaact ttttaatata actaaagaag tgggcatacc
     3001 agtaatttgg gcattgtata taaatcataa tgaaaatgct agtattcgaa gtttttcagc
     3061 tgcaggctgt catattaatc ctttaagtgc aattgaatca gcaatttttg aagtaataac
     3121 ttctgtgcca acttttgaaa gattaattga agttagaaat aaagagtcat ttattgagga
     3181 aattcagaat aatttttctt taatacgaga tattgacgat catatattgt atcatgctca
     3241 tgcggattat aaaaataaaa tgcccttttt atttaaaaat aattatagtg aatatcaaaa
     3301 tgaatttgat gagaatagaa tttttaatac ttattcacat gcaacgctta catcagattt
     3361 acaggcacta ataaaaatta ttgaaaaaaa atttggagat atatatgtag tgaatataag
     3421 tgataaagtt tccgaaaaat ttggacttta ttgtgttaaa gtgttgattc ctggacttct
     3481 taatatgtca tttggacatt caaatcggcg tgtaaatata gagaggataa aaaattatgt
     3541 agattataga gggggaaatg taaatagtat tacattgaat tccataaacc atcttcccca
     3601 tccatttcca taagattata tataaaggaa taataaatga aaagagattg gaaatcaaag
     3661 catttatttt atcatgatgt tcaacaattt gataaattgg taatctattt atcaaaattt
     3721 atggataaat tagttaatca gaatcagatt gaaaaatatt tctatattcg ttattgggaa
     3781 ggggggcctc atattcgatt aagatatttg gtgagggata cacaaaattt tgaaacaatt
     3841 gatagtatga taaataagta tgcttataaa ttttgggaaa ataatcctcc aatgaagtta
     3901 ttaaataaga ccgattataa gaaaatttat aatgagagta ttgataacaa cggaaagatg
     3961 gatagaaaat tgaggaaaaa tgcgactatt gaaaacattg cctatatacc ggaaatagag
     4021 agatatggcg gggaagaagc tctgatagta agcgaagatc tattttgtat ctgtagtcag
     4081 gtttcaactg cgttacttaa tgtggtagat aactat
//
LOCUS       DAVBHS010000153         4037 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVBHS010000153 DAVBHS010000000
VERSION     DAVBHS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.153"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(115..390)
                     /locus_tag="VQ651_001828"
     CDS             complement(115..390)
                     /locus_tag="VQ651_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET2754783.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(496..1335)
                     /locus_tag="VQ651_001829"
     CDS             complement(496..1335)
                     /locus_tag="VQ651_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2754784.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVIVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1477..2325
                     /locus_tag="VQ651_001830"
     CDS             1477..2325
                     /locus_tag="VQ651_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET2754785.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2418..3335)
                     /locus_tag="VQ651_001831"
     CDS             complement(2418..3335)
                     /locus_tag="VQ651_001831"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET2754786.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3772..3977
                     /locus_tag="VQ651_001832"
                     /pseudo
     CDS             3772..3977
                     /locus_tag="VQ651_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1255 a          812 c          651 g         1319 t
ORIGIN      
        1 tacaatattt tcggaacatt caacttttta actctattta ttactagatt tcataattaa
       61 aaagcctact gaccaagcta gaaagcttga tacaataggc tttttaaaga ctgattattt
      121 aacagcgtct ttaagagctt taccagcttt gaatgctggt actttagaag ctgcaattgt
      181 catttcttta ccagtttgtg ggttgcgacc tttacgttct gcacgctcac gaacttcaaa
      241 gttaccaaaa ccgatcaatt gaactttttc accagctgca agatagtcag ctactgctgc
      301 aaatacagct tcaactgctg ctgctgagtc tttcttagtc aattctgtag cttctgctac
      361 tttagcgatc aaatcttgtt tgtttgccat gttaatgatt cctccagata atttctaatt
      421 aacaaatata atcatatcct aaaatccctt ttaggtcaag ttaaaaacgc tatttcttcc
      481 aattttattt attttttagg agtggtaacg tatcaaaata gcccaagcgt tctcacccgt
      541 gtgagtttga ataatggaac ccgtttccaa aacagaaatt ggcttttcaa cataagcttg
      601 taagctttct ttcatctctt ttgcccaatc atcactacca gaatatgaaa ttccaatctc
      661 tgctacagca cgttcagaaa gcgatgttat caactcatct aaccattttt taaatgtttt
      721 agttccacga cctttaacca ttggctgcaa ttcatggtct ttcatttgca tgacaacacg
      781 gatattgaga agggagctca acaagccagt tacacggcca attcgtccac ctttgacaag
      841 attttccaaa gttgaaacac caatataaag ctctgtatgg tttttaacct cttctacatg
      901 agataaaatt gcctccatat ctttaccttc ttgagctaac ttcgcagcct caacaacttg
      961 gaatttcagg gcttggtcag tgaaggaact atcaacaaca atcacatctg cagtagatag
     1021 gctagcacct tggcgtgctg cttctaccgt acccgaaaga gcatgggaca tatgaatagc
     1081 aagaatctgg ccaccatctt tgcataggtc ttcaaaaatc tcagcaaaga cacctacagg
     1141 tggctgactt gttttcggaa gattcttact ttcttgcatc aactgaagaa atttaccttc
     1201 ttctttcaaa tccgcatcag aataaacaac attatcaatc attacagata atggaacaat
     1261 tgtaatatct aattgcttta ctagttcagg ttcaatagta acagatgaat cggttacaat
     1321 cttaattttt gtcatagtat caatctttct attttaggat tcagattggt ttccttactt
     1381 ctaattatat caaaaaaaag attaaaaatc ctaatggagt caatcaaatt ttccgtaaaa
     1441 tttgatataa tcaacttata agaaaagagg tgtcctatga ttaaaaaaat ttaccccatt
     1501 tttaccattt tactaggtgc tgctatttat gcttttggac tgacttattt tgtagttccc
     1561 catcatctct ttgaaggagg ggcgacaggt attaccctca tcacatttta tctttttaaa
     1621 atccctgttt ccctcatgaa cctgctgatt aatattcccc ttttcatcct agcttggaag
     1681 atctttggag ccaaatccct ctattctagt ttactaggaa ccttagcttt gtccggctgg
     1741 ttagcttttt ttgagcatat tccccttcat attgatcttc aaggtgattt actaataaca
     1801 gcccttatag cgggaatcct attgggaatt ggccttggaa ttatttttaa tgctggaggt
     1861 acaactggcg gaactgatat tctagctcgt attctcaaca aatacactca tatatccata
     1921 ggaaaactgc tctttatctt agatttttgt attctcatgt tgattctcct aatcttcaag
     1981 gatttgagat tggtttccta cacgcttttg tttgatttta ttgtttctcg tgttattgat
     2041 ttgattggtg aaggaggata tgccggcaaa ggctttatga ttatcacaaa acgtcctgac
     2101 caacttgcta aggcgattaa tgatgacctc ggaagaggtg ttacttttat ttctggtcaa
     2161 ggctactata gtaaagaaaa tttgaaaatc atctactgta ttgtcggaag aaatgaaatt
     2221 gtgaaaacga aggaaatgat tcatcgaatc gatcctcaag cctttataac tattacagaa
     2281 gcccatgaaa tcctaggaga aggcttcacc tttgaaaaag aataaaaaga ggtaatgtcg
     2341 tgacctcaaa agttagacta aatcatctat cttttgggtt acagacaacc tcttttttgt
     2401 tttatttact caagctctta agaccaattc cgagttactt cttcatcagc ctttaactga
     2461 tccactaatt ggtcaactga gtcaaatttg gtcatatcac gaatgcgatc aagccaataa
     2521 accatgacgg tttccccata aatatcttga ttaaaatcaa aaatattgac ttcaaaacgt
     2581 gcttcttctc catcaaaggt cacatttttc ccgacactag ccatagcacg atacttctgt
     2641 ctttgaatct caacatcaac gacataaacg ccatctgctg gcatataagt acggtctaaa
     2701 agcactaaat tcgctgtcgg ataaccaatt gtacgaccac gagcattacc atgaaccacc
     2761 atacctcttg atggaagcgg tgcccccaaa agttttcctg cttctttcac atttccatct
     2821 aaaatagctt gacggatacg agttgaacta atctttcctt tctcatcttc tacaggtgga
     2881 acaatgataa cttctccatc aaagtaatcc tttaaatctt ctgctgtttt tttgtcagaa
     2941 ccaaatgtat aatcaaaacc tgcaacaata attttggcat tcatagcctt gatataagtt
     3001 gcaaagaatt cttgtgcagt gagactagcg aattgactac taaaatcaag gagatataat
     3061 tcttctacac cttcgcgctt taattttctt tcacgttcag cagggttcaa aatatgcaaa
     3121 aacaaatccg gatgataagg ctctaaagcg atctttggag attcattaaa ggtcataacg
     3181 acgataggta ataaatcctt tctcgcagcc ttgttggcaa cacgaaataa ttcttgatgc
     3241 cccttatgta tgccatcaaa atagccgaga acaacgactg aatcagatgg tgtgccaata
     3301 tctttttggt tttttatagg aatagtaata atcataaaat aattatatca tagcgatagc
     3361 tatttctgga acagaaaatc tgaaatgttg tttttttcac atgaagtgta cctgttttca
     3421 aaaagcactt tattctatcg ttgcttaact atgaactttg caatattctt ctcaaaaact
     3481 tgtaggacat cttcaaaatt ttgcaaggag tgattagact tgttcggtaa ccataaagtg
     3541 tcatactatg ttatgtatga aaaagcaatg caactaactc ctgagaactt taaattacta
     3601 attggtgccg aaaaggtaga atttagaatc gaggtacacc tatggctgta aaatttacaa
     3661 aatgagacaa cttgggcaag atgtttgaag aatttcctaa actccctgat ttgaagcaag
     3721 tcactttccc tgatgacaaa gaaaaaagcc aaaacagcaa agaaaaacta gatgactgct
     3781 ttccaacaac tcccatctag tgtgcttcag actgggctat ttttctctcc atctgttagc
     3841 ttggattctc agaccgtttc agctaaagaa tatcttttcc cttatcagaa ggaacggctc
     3901 aaaccattca gacaagtgaa gggacgacaa gccaatattt gaaaccagat agcagttctt
     3961 atagtcaatt gaaataaaat ctgaagaaat cgagtaggaa actcatatca atttttaaca
     4021 gtgttctatt ccagatt
//
LOCUS       DAVBHS010000154         4007 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVBHS010000154 DAVBHS010000000
VERSION     DAVBHS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.154"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            361..1017
                     /locus_tag="VQ651_001833"
     CDS             361..1017
                     /locus_tag="VQ651_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2754787.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDESIDLPALREKFEANGIQVEEIARTSAKVL
                     "
     gene            1125..2220
                     /gene="prfB"
                     /locus_tag="VQ651_001834"
     CDS             join(1125..1196,1198..2220)
                     /gene="prfB"
                     /locus_tag="VQ651_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET2754788.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            2238..2930
                     /gene="ftsE"
                     /locus_tag="VQ651_001835"
     CDS             2238..2930
                     /gene="ftsE"
                     /locus_tag="VQ651_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET2754789.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            2923..3849
                     /locus_tag="VQ651_001836"
     CDS             2923..3849
                     /locus_tag="VQ651_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2754790.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
BASE COUNT         1266 a          651 c          910 g         1180 t
ORIGIN      
        1 catcaaatcc agaggattgt tttagccggc agataaagat tacgaagtaa tcatcaatat
       61 agtccggggg ccctttttag tcggtggatg aagattgcga agcaatcatc aaatccagag
      121 gattgtttta gccggtagat tgagattgca aacaaatctg catctacatt gaaagcttaa
      181 tttctaataa ttgaaaaaat cgaatgaaaa gtttcttacc ttcattcaca gagctcgatt
      241 ttagagctct ttttgctagc ttattcatac ttttctaaat tttgaaaaag aaatgtaagc
      301 gtttgataga tttgcaaaaa gattgtataa tagggataag aatagaaaag gagaagtctc
      361 atggcagtta aagattttat gacccgcaag gtagtttata ttagtccaga tacaacagta
      421 tctcatgcag cagatttgat gagagagcaa ggtttgcacc gtctgcctgt tatcgaaaat
      481 gatcaattgg ttggtttggt gactgaggga accattgcac aagcaagtcc atctaaagca
      541 acaagtcttt ctatctatga gatgaattat cttctgaata agacaaaagt aaaagatgtc
      601 atgattcgcg atgttgtcac tgtctcaggc tatgctagtc tagaagatgc aacttatctg
      661 atgttgaaaa ataagattgg tattctccct gtcgtagata accatcaagt atacggagtt
      721 attactgacc gtgacgtttt ccaagccttt cttgaaattg caggttatgg cgaagaaggg
      781 attcgtgtac gctttgttac agaagatgaa gttggtgttc ttggaaaaat tgtttctttg
      841 attgtagaag aaaatttgaa tatctcccat acagtcaata ttccgcgtaa ggatggtaag
      901 gtgattatcg aagtgcaaat cgatgaatca attgatttac cagccttgag agaaaaattt
      961 gaagcaaatg gtattcaagt ggaagaaatc gctcgcactt cagcaaaagt cttgtaagaa
     1021 gggaagtcca aaggcttctt ttttcatgaa aaggggatta gagcaaaaga tggaaagaaa
     1081 tgataaaata tgctataatg aaataatgta aaaaaggagt atttatggac atttcagtaa
     1141 ttcgtcagaa aattgacgca aatcgtgaaa aattagcttc tttcaggggg tctctttgac
     1201 ctcgaagggc tagaggaaga gattgccatc ttggaaaaca agatgacaga acctgatttt
     1261 tggaacgata atattgcggc ccaaaaaacg tcgcaagaat tgaatgaatt aaaaaacact
     1321 tacaatacct tccataagat ggaagagttg caggatgaag tcgaaatttt attggatttt
     1381 ttggctgaag acgagtcagt gcatgatgaa ctggtagcgc agttagccga acttgataag
     1441 ataatgacca gctacgagat gactctactc ttgtcagaac cttatgacca caacaatgcc
     1501 atcttggaaa tccatccagg ttctggtggt actgaggcgc aggactgggg tgatatgttg
     1561 cttcgtatgt atactcgtta tggtaatgct aaaggcttta aagtggaagt gttggattac
     1621 caagcaggtg atgaggctgg tattaagtcg gtaactttat catttgaagg gcctaatgcc
     1681 tatggtctcc tcaagtcaga aatgggtgtt caccgcttag tgcgaatctc accatttgac
     1741 tctgccaaac gtcgccatac ctctttcaca tctgtagaag tgatgccaga attggatgat
     1801 actattgaag tggaaatccg tgaagatgat atcaagatgg ataccttccg ttcaggtggt
     1861 gccggtggac aaaacgtcaa taaggtttca acaggtgtac gtttaaccca cattccaact
     1921 ggaattgttg tccaatcaac agtggatcgt acccagtatg gaaatagaga tcgtgccatg
     1981 aagatgttgc aggctaagct ctatcaaatg gagcaagata agaaggctgc ggaggtagat
     2041 tctctcaaag gtgagaaaaa ggagatcact tggggaagcc aaatccgttc ttatgtcttc
     2101 acgccttata ctatggtaaa agatcaccga actagctttg aggttgctca ggtagataag
     2161 gttatggatg gggacctaga tggttttata gatgcttatc tcaagtggcg aattagctaa
     2221 gatagaaagg aactcacatg tcaattattg aaatgagaga tgtcgttaaa aaatacgaca
     2281 acggaacgac tgctctacgc ggtgtttcgg tcagcgttca accgggggaa tttgcttaca
     2341 tcgtaggacc ttcaggagca gggaagtcaa cttttattcg ttctctgtat cgtgaagtaa
     2401 aaatcgataa aggaagtcta tcagttgctg gttttaatct ggttaagatc aaaaagaaag
     2461 atgtcccgct tctacgtcgt agtgttgggg ttgtcttcca agattataaa ttgttaccaa
     2521 agaaaactgt ctatgaaaat attgcttacg ctatggaagt aatcggggaa aatcgccgta
     2581 atatcaaaag acgagtgatg gaagttttgg acttggttgg attgaagcat aaggttcgtt
     2641 ctttcccaaa tgaactctca ggtggggagc agcagcgaat tgcgattgcg cgtgcaattg
     2701 taaataatcc caaagtattg atagctgatg agccaacagg aaatctggat ccggataatt
     2761 catgggaaat tatgaatctc ttggaacgga ttaacctaca agggacaact attttgatgg
     2821 cgactcataa tagccagatt gtaaatacct tgcgccaccg tgtcattgcc attgaaaatg
     2881 gccgtgtcgt tcgtgacgaa tcaaaaggag agtatggata cgatgattag tagatttttt
     2941 cgccatttat ttgaagcctt aaaaagtttg aaacgaaatg gttggatgac agtagctgct
     3001 gtcagttcag tcatgattac tttgaccttg gtggcaatat ttgcatctgt tattttcaat
     3061 acagcgaaac tagctacaga tattgaaaat aatgtccgtg tagtagttta tatccgaaag
     3121 gatgtggaag ataatagtca gacaattgaa aaagaaggtc aaactgttac aaataatgac
     3181 taccacaagg tatatgattc tttgaagaac atgtctacgg ttaaaagtgt taccttttca
     3241 agtaaagaag aacaatatga aaaattaacc gagataatgg gagataactg gaaaatcttt
     3301 gaaggagatg ccaatcctct ctatgatgcc tatattgtag aggcaaacac tccaaatgat
     3361 gtaaaaacta tagccgaaga tgctaaaaaa attgaaggtg tctctgaggt tcaagatggc
     3421 ggtgccaata cagaaagact cttcaagtta gcttcattta tccgtgtttg gggactaggg
     3481 attgctgctt tgttaatttt tatcgcagtt ttcttgattt caaataccat tcgtattacc
     3541 attatttccc gcagtcgcga aattcaaatc atgcgcttgg ttggagctaa aaacagttat
     3601 atccgtggac cgttcttgtt agaaggagcc tttatcggtt tattgggagc tatcgcacca
     3661 tctgttttgg tctttattgt ttatcaaatt gtttaccaat ctgtcaacaa atcgttggta
     3721 gggcaaaatc tatccatgat tagtccagat ttatttagtc cgttgatgat tgccctacta
     3781 tttgtgattg gggttttcat tggttcattg ggatcaggaa tatccatgcg ccgattcttg
     3841 aagatttagg taaaatagct gcttttatga ggagattgta aaatctcctt ttttgctaca
     3901 agagtttttg aaaagagatg cgtagaagaa aagagcttcc aaagaagtcc cccagagaag
     3961 acttcttgat ggctaatttg aacttgaaat ttggcgcact agaaatt
//
LOCUS       DAVBHS010000155         4005 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVBHS010000155 DAVBHS010000000
VERSION     DAVBHS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.155"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..1455)
                     /locus_tag="VQ651_001837"
     CDS             complement(<1..1455)
                     /locus_tag="VQ651_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET2754791.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMATLTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIAT"
     gene            complement(1601..1888)
                     /locus_tag="VQ651_001838"
     CDS             complement(1601..1888)
                     /locus_tag="VQ651_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754792.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     MQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(1899..2549)
                     /locus_tag="VQ651_001839"
     CDS             complement(1899..2549)
                     /locus_tag="VQ651_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2754793.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            complement(2553..3194)
                     /locus_tag="VQ651_001840"
     CDS             complement(2553..3194)
                     /locus_tag="VQ651_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754794.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLIVSRLEEKFKKG"
     gene            complement(3187..3978)
                     /locus_tag="VQ651_001841"
     CDS             complement(3187..3978)
                     /locus_tag="VQ651_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754795.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLEWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLKKYEDNV"
BASE COUNT         1354 a          806 c          684 g         1161 t
ORIGIN      
        1 ggttgcgatg gtaacggcac cacgttgacc agcattcatg atgatttggg cttctctata
       61 gtggtttttg gcattcaaga cttcgtgagg aacaccagct gcaaccaatt tcttagaaat
      121 gtagtcacta gtttcaaccg ctactgtacc aaccaagaca ggttgaccct tttggtaacg
      181 agccttaacg tcttcgacaa ccgctttaaa cttagattcg atacttgcat aaagaaggtc
      241 tgagtggtca atacgttgaa caggacggtt tgttgggatt ggaataacac gaatgttgta
      301 gatttcacgg aattcttctt cctcagtctt acctgtaccc gtcataccag acaatttctt
      361 gtacatacgg aaaaggtttt ggtacgtgat tgaggcagat gtcttggttt catcctggat
      421 tggcacacct tctttggctt caatagcttg gtgcaatcca tcagaataac gacgaccttc
      481 catggtacga cctgtaaatt ggtcgacaat caagatttct tgctcttcgc tcaccacata
      541 gtcaatatcg agaagcatga tgtagttggc acgaagggcg ttatcgataa agtgagtcaa
      601 agccacgttt tcgatgtcat agaggttttc aagtttgaag tagctttcag ccctgtcaat
      661 ccctgaatca gacaaaccaa tagtcttaga ctgcacatcg atgatgtagt catctttgtt
      721 caaagatttt acatagtggt ctgccatgtg atacaactga ctggtttcaa ccgcattggc
      781 acctgataca atcaaaggtg tacgagcctc gtcaatcaag atagagtcaa cctcatcgac
      841 caaggcatag ttaagcggac gttgtaccat gttttcggcg cgaacgacca tgttgtcacg
      901 aaggtagtca aatccgattt ctgagttagt tgagtaagta atatcacact cataggcttc
      961 ttttttctcc attggagatt tggtagccaa gttaatccct actgacaaac caagccaaga
     1021 gtacaattca cccatctcag tcgcgtcacg ttctgacagg tattcattaa ccgtaactac
     1081 gtgaacccct ttacctgaaa gggcattgag gtataccggc atggtcgcag tcaaggtttt
     1141 cccttcccct gtacgcatct ctggcacgtc accatggtga agaacaatcc cccccatgac
     1201 ctgaacctta taagggaaga gacctaggac acgtttggca ccttcacgga caaccgcaaa
     1261 tgcttcgtaa agcaatgaat ccagtgattc tccattttga taacgttcct taaattcaac
     1321 tgtttttgct tttagttggt cgtcagtcaa agtagccatt tggtcttcgt atttgaaaac
     1381 cttgtcagcc atcttttcca gacgacggat ttctccttta tcattttcga taattgtttt
     1441 taaaatatta gccatgtttt tccttacttt aaattccgaa tattttagaa tgttccttta
     1501 attttagcac aattactgat tattttcaag gaagaatccc cattttgaaa aggaaaaaga
     1561 ggttagtttt caagctaacc tctccgactt ttatgactgt ttaattgcca aaaatcggca
     1621 aaatggcaaa tgtgataaat aagttaatcc aaagagaaag acagcaaatc agtccaaaaa
     1681 caaagagact gactttcttg gacagcaagg ccatgagaat ggacagaatt ccaaaagcta
     1741 gcaagatttt ctgcatgatg aagatgtcaa tccttacccc cagaatcgct ctatcccaag
     1801 ggataaagaa gaaagacagc cagatcagca gaactaaacc aatataaacc ttggaatagc
     1861 gtgtaataaa tgattttaga tgtgccataa gccatctcct aggattgtat atcctttaca
     1921 tattgtatat gtccattttc gacaactaat acatctgtac aaaggtctga aatatcttcc
     1981 gatatgtgcg acgtcaggat aaccagacca ttttcttttt tcaggtaaga taaaatgacc
     2041 tgtttggtta acctcacact cttctcatcc aaagcattca taggttcatc gagaaagagt
     2101 atagaaggag aggaaataaa ggcttgaatc aaggccattt tttgctttgt tcccaaggat
     2161 aaatgacggt attcaacgtc ttcaaattcc tgtaaatcat agtattgaat ccaataggca
     2221 attctttttt ccgtaacttt agatgaaaaa taccttaaca gttccaaatt ttctctcagg
     2281 gataaatgag ataaaaactc gactttttca attaaaattc ctgcatcctg aatataatga
     2341 ttttttaccc cgtaaacttt accatcttga agaacttttc ctttgtcaag cttaatataa
     2401 ccagcaagta tctttaaaag aaccgtcttg ccagatccat tatcaccttt aagtccataa
     2461 atttttcctg attgaaacac caaattcaac tgatttaaaa taggtctttt cccataaatt
     2521 ttctgtccat ttataatttc aagtctcatc gtttatcctt tcttaaattt ctcttccaag
     2581 cgactaacaa tcaacataac taataagctt aagagtacta aatacactgt tacaatatga
     2641 tccatcatcc attcttgtcc cacaaatgcc aaaaaataga gaaaaaaaga aaaaccagcg
     2701 cccgcactaa tcagtgctat cagataagac agtagtaagt acaacatcat taactgagca
     2761 agtaaaaaga aaagtgcgac tgttttgaaa tgaattccta gcggaagtaa accaagaaac
     2821 cagacaaaca aatcttgagt aaaaaattta gaaatgacaa ccaatcgttt ccttgtataa
     2881 tcgaaaaagg agcttgattg atagcgaatc aagtgatggt aatggtctgc caaatcactt
     2941 tcaagtaaaa tgacagagag ccatcctacc atgaaaagcg aaaggtttaa caagagtttg
     3001 gggatttcta aattcgcttc agaatagtca ttataggaaa aaattgaaat caattctctc
     3061 ttgaaaaaga taacagcatc ctgcttttga accgctgttg acaatagata gtttatcaaa
     3121 atcatcatca gaactatacg aagtagtaaa acgatctttc tctttagcca aatattgtta
     3181 aaattcttaa acattatctt catatttttt aagtaccata taagggacaa cataaaggat
     3241 ataaggagta aagagtttca tcaaacttac atccccatag ctagcttgca tcaaactcgt
     3301 cataggaacc atatagtaag gcaaggcact atagagcagc atagaaccaa gccaaagaaa
     3361 catcaaatag gtgattgccg aacgagtcac attccccaca taatcaacta tttgtaaaaa
     3421 atagattgca ttgatgaaaa taccgattag taggacacaa gtaaagaaca aatagctttt
     3481 tatctctcca tctaggagtc tttgtaaacc actcccatca gaaaatagag aattccattc
     3541 aagaggagaa aaagtcccaa agaaataggt tataattgca attatcagca cagtcactaa
     3601 atatatcaaa caagggatac ttgcaacttg caaagtcaac tttcttttta acccttgata
     3661 gctcacttct cttccaatac tcaatcgtaa aactttattt ttcagctcaa tatattgaaa
     3721 acctagcaaa acaatgatga ttggaataat aaatgattga taaaaaccta gattgaactg
     3781 gagaatacga aaatctaatg ctgattttaa taagtcattt ttaattgctt gtccatcgaa
     3841 tttagctaga tcgggcgcat gaatatcaaa aaagaaataa gtcatcaaaa catcacgata
     3901 aaccaaagca agaaaaagca aagtaagcaa gacataaaca aatttatgcc ttgccaaagt
     3961 taagagagga atcttcataa gccacctcct acaaatagaa agtaa
//
LOCUS       DAVBHS010000156         3999 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVBHS010000156 DAVBHS010000000
VERSION     DAVBHS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.156"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            206..2107
                     /locus_tag="VQ651_001842"
     CDS             206..2107
                     /locus_tag="VQ651_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2754796.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINCVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
     gene            2110..2973
                     /locus_tag="VQ651_001843"
     CDS             2110..2973
                     /locus_tag="VQ651_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET2754797.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            3053..>3999
                     /locus_tag="VQ651_001844"
     CDS             3053..>3999
                     /locus_tag="VQ651_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2754798.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQVIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFILVTFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTI"
BASE COUNT         1197 a          679 c          882 g         1241 t
ORIGIN      
        1 gcgtacgtaa ataatgaaaa gaattaaata ataggcttgt gcgataacta tttgaaacaa
       61 gattagctgg ttagaaaatc caacaagata gtgaaggaat ctaatataaa atatacataa
      121 tttagtttat tttatgttat aaaagttgta ctaaaagact tacgattatc ttgaatgcct
      181 tagggaaaca tgctatacta ctcttatgat tattttacaa gctaataaaa ttgaacgttc
      241 ttttgcagga gaggttcttt tcgataatat caacctgcag gttgatgaac gagatcggat
      301 tgctcttgtt gggaaaaatg gtgcaggtaa gtctactctt ttgaagattt tagttggaga
      361 agaggagcca actagcggag aaatcaataa gaaaaaagat atttctctgt cttacctagc
      421 ccaagatagc cgttttgagt ctgaaaatac catctacgat gaaatgcttc atgtctttaa
      481 tgatttgcgt cggacggaga gacaactgcg tcagatggag ctggagatgg gtgaaaagtc
      541 tggtgaggat ttggataaac tgatgtcaga ttatgaccgc ttatctgaga attttcgcca
      601 agcaggtggc tttacctatg aagctgatat tcgagcgatt ttgaatggat tcaagtttga
      661 cgagtctatg tggcagatga aaatagctga actttcaggt ggtcaaaata ctcgtttggc
      721 tctagctaaa atgctccttg aaaagcccaa tctcttggtc ttggacgagc caactaacca
      781 cttggatatt gaaaccatct cctggctaga gaattacttg gtaaactata gcggtgccct
      841 cattatcgtc agccacgacc gttatttctt ggacaaggtt gcgacaatta cgctagattt
      901 gaccaagcat tccttggatc gctatgtggg gaattactct cgttttgtcg agttgaagga
      961 gcaaaagcta gctactgagg caaaaaacta tgaaaagcaa cagaaggaaa tcgctgctct
     1021 ggaagacttt gtcaatcgca atctagttcg tgcttcaacg accaaacgtg ctcaatctcg
     1081 ccgtaaacaa ctagaaaaaa tggagcgttt ggacaagccg gaagctggca agaaagcagc
     1141 caacatgacc ttccagtctg aaaaaacgtc gggcaatgtt gttttgactg ttgaaaatgc
     1201 ggctgttggc tatgatgggg aagtcctgtc acaaccgatt aacctagacc ttcgtaagat
     1261 gaatgctgtt gctatcgttg gtcccaatgg tatcggaaaa tcaaccttta tcaaatcaat
     1321 cgtggatcaa attccgttta tcaagggaga aaagcgcttt ggcgctaatg ttgaggttgg
     1381 ttactatgac caaacccaaa gcaagctgac accaagtaat acggtgctgg atgaactctg
     1441 gaatgatttc aaattgacac cagaagttga aatccgcaac cgtcttggag ccttcctttt
     1501 ctcaggagat gatgttaaaa aatcagtcgg catgctatct ggtggcgaaa aagctcgttt
     1561 gcttttagct aaattgtcta tggaaaacaa taactttttg attctggatg agccgaccaa
     1621 ccacttggat attgatagta aggaagtgct agaaaatgcc ttgattgact ttgatggtac
     1681 cttgcttttt gtcagtcatg atcgttactt tatcaattgt gtggcaactc atgttttgga
     1741 attgtctgag aatggttcaa ctctctacct tggagattac gactactatg ttgagaagaa
     1801 agcaacagca gaaatgagtc agactgagga agcttcaact agcaatcaag caaaggaagc
     1861 aagtccagtc aatgactatc aggcccagaa agaaagtcaa aaagaagttc gcaaactcat
     1921 gcgacaaatc gaaagtctag aagctgaaat tgaagagcta gaaagtcaaa gccaagccat
     1981 ttctgaacaa atgttggaaa caaacgatgc cgacaaactc atggaattac aggctgagct
     2041 ggataaaatc agccatcgtc aggaagaagc tatgcttgag tgggaagaat tatcagatca
     2101 ggtgtaaaga tggaacatct tggaaaagta tttcgtgaat ttcgaacaag tggaaattat
     2161 tctttaaagg aagcagcagg cgaatcctgc tctacctctc agttatctcg ctttgagctt
     2221 ggggagtctg acctggcagt ctcccgtttc tttgagattt tggataacat tcatgtaaca
     2281 atcgaaaatt tcatggataa ggcaaggaat tttcataatc atgaacatgt gtctatgatg
     2341 gcacagatta tcccacttta ctattcaaac gatattgcag gttttcaaaa gcttcaaaga
     2401 gaacaacttg aaaagtctaa gagttcgacg acttcccttt attttgagct gaactggatt
     2461 ttgctacaag gtctgatttg tcaaagagat gcgagttatg atatgaagca ggatgatttg
     2521 gataaggtag cagattatct cttcaaaaca gaagaatgga ccatgtatga gttgattctt
     2581 ttcggtaacc tctatagttt ctacgatgta gactatgtca ctcggattgg tagagaagtt
     2641 atggagaggg aggaatttta ccaagagatt agtcgccata agagattagt gttgattttg
     2701 gccctcaatt gttaccagca ttgtttagag cattcttctt tttataatgc caactatttt
     2761 gaggcttata cagagaagat tattgacaaa ggtattaagc tttatgagcg taatgttttc
     2821 cattatttaa aaggttttgc cttatatcaa aaaggacagt gtaaagaagg ctgtaagcag
     2881 atgcaagagg ccatgcatat ttttgatgtg ttaggtcttc cagagcaagt agcctattat
     2941 caggaacact acgaaaaatt tgtcaaaagt taattttccc aaataaggga aaaaataaaa
     3001 agctcctttc ggttttgata caatagtttc aaaatttgag aggagcttta atatgaatcg
     3061 ctatgcagtg cagttgatta gccgtggggc tatcaataaa atgggaaata tgctctatga
     3121 ttatggaaat agtgtctggt tggcttctat ggggactata ggacagacag ttttaggaat
     3181 gtatcagatt tctgagctcg tcacatctat tctcgtcaat ccctttggcg gagttatttc
     3241 agaccgtttt tctcgtcgta agattttaat gacggcagat cttgtttgtg ggattctttg
     3301 tctggctatt tctttcataa ggaatgatag ctggatgatt ggcgctttga ttgttgctaa
     3361 cattgtgcag gttattgctt ttgccttttc tcgcacagcc aataaagcta tcataactga
     3421 agtggtggag aaagatgaga ttgtgatcta taattctcgc ttagagctgg ttttgcaggt
     3481 tgtaggtgtt agctctcctg ttctttcctt ccttgtttta cagtttgcaa gtctccatat
     3541 gacgctactg ctagactcgc tgactttttt cattgctttt attctagtga ctttccttcc
     3601 aaaagaggaa gcaaaagttc aagagaaaaa ggcttttact gggagagata tttttgtaga
     3661 tatcaaggat gggttacact atatctggca tcagcaagaa attttcttcc ttttgctggt
     3721 agcttccagc gttaatttct tttttgcagc ttttgaattt ctacttccct tttcgaatca
     3781 gctttacggg tcagaaggag cctatgcaag tattttaact atgggggcta ttggttccat
     3841 cattggggct cttctagcta gtaaaattaa agctaatatt tataatcttt tgattttact
     3901 ggctttgaca ggtgtcggag tttttatgat gggattacca cttccaactt ttctttcctt
     3961 ttctggaaat ttagtttgtg aattgtttat gacgatttt
//
LOCUS       DAVBHS010000157         3938 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVBHS010000157 DAVBHS010000000
VERSION     DAVBHS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.157"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            484..1137
                     /gene="rr06"
                     /locus_tag="VQ651_001845"
     CDS             484..1137
                     /gene="rr06"
                     /locus_tag="VQ651_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HET2754799.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            1134..2474
                     /gene="hk06"
                     /locus_tag="VQ651_001846"
     CDS             1134..2474
                     /gene="hk06"
                     /locus_tag="VQ651_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HET2754800.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILFLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            2557..3084
                     /locus_tag="VQ651_001847"
     CDS             2557..3084
                     /locus_tag="VQ651_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HET2754801.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            3163..>3938
                     /locus_tag="VQ651_001848"
     CDS             3163..>3938
                     /locus_tag="VQ651_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HET2754802.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEGVRSGD
                     TPKVTSSGDEVESHLKKILSEIQTQLDRKRHAKTVALINKLQDIKRTYSYKLNVLEEK
                     PKAELTSKTKEELTAAFEQFKKDTLPTEPGKKVAEAEKKVAEAEKKAEDQKEEDRRNY
                     PTNTYKTLELEIAEFDVKVKEAELELLKEKAKKPLNEGTIKQAKEKVESKKAEATRLE
                     NIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPAPAPQPE"
BASE COUNT         1361 a          552 c          819 g         1206 t
ORIGIN      
        1 aaactaaaac ctatagcttc taaacgatcc gtggttttca ccattcaaca caaaattcat
       61 atgtttatta ccctccgtcg tatttgtctt agagcgtgtg tagtagaaaa agagcagtct
      121 tatctgaaat ttttattctt tcaaaagaga cctgtttctt ttttgcatgt caaatccgtt
      181 ctagctggta tttgaaaaat caaactaata ttcaatgaaa atcaaagaac aaactaggca
      241 gctagccaca ggttgctcaa aacactgttt tgaggttgta gatagagctg acgtagtttg
      301 aagagatttt cgaagagtat aagctgcaag atgaatgatt ttcttgtatt gacgttgttg
      361 ttgacaaaaa gtagcggata aatgaaatcc attccattat catagatgat aggctggtag
      421 gaaattttca aatagcatac aggaaataga tgtatggagt tctggtagta gaaagggaga
      481 gagatgaaca ttttagttgc agatgacgag gaaatgatta gagaaggaat tgcagcattt
      541 ctgacagaag agggttatca tgtcattatg gctaaggatg gacaagaggt cttggaaaaa
      601 tttcaagatc tccctatcca tctcatggta ttggatttaa tgatgcctag gaagagtggt
      661 tttgaagtgt taaaagaaat caatcaaaag cacgatattc ctgtcatcgt cttgagtgct
      721 ctgggagatg aaactactca gtcacaggta tttgatctct atgctgatga tcatgtgaca
      781 aaaccttttt ctttggtact gcttgtcaag cgtattaagg cgcttatcag acgttactac
      841 gtcatagagg atatttggcg atatcaggat gtaacagtgg attttacctc ttacaaagca
      901 cattataaaa atgaagaaat tgatctcaaa ccaaaggaat tactggtact aaagtgtttg
      961 attcagcata aaaatcaagt tttaagtaga gagcagatat tggaagaaat ttcaaaagat
     1021 gtagctgatt taccttgtga tagggtcgtt gatgtctata ttcgtactct tcgcaaaaaa
     1081 ttagctttag attgtatcgt gactgtgaaa aatgttgggt ataagattag cttatgataa
     1141 aaaatcctaa attattaacc aagtcttttt taagaagttt tgcaattcta ggtggtgttg
     1201 gtctagtcat tcatatagct atttatttga cctttccttt ttattatatt caactggagg
     1261 gggaaaagtt taatgagagc gcaagagtgt ttacggagta tttaaagact aagacatctg
     1321 atgaaattcc aagcttactc cagtcttatt caaagtcctt gaccatatct gctcacctta
     1381 aaagagatat tgtagataag cgactccctc ttgtgcatga cttggatatt aaagatggaa
     1441 agctatcaaa ttatatcgtg atgttagata tgtctgttag tacagcagat ggtaaacagg
     1501 taacagtgca atttgttcac ggggtggatg tctacaaaga agcaaagaat attttgtttt
     1561 tgtatcttcc atatacattt ttggttacaa ttgctttttc ctttgttttt tcttattttt
     1621 atactaaacg cttgctcaat cctctttttt acatttcaga agtgactagt aaaatgcaag
     1681 atttggatga caatattcgt tttgatgaaa gtaggaaaga tgaagttggt gaagttggag
     1741 aagttggaaa acagattaat ggtatgtatg agcacttgtt gaaggttatt catgagttgg
     1801 aaagtcgtaa tgagcaaatt gtaaaattgc aaaatcaaaa ggtttccttt gtccgtggag
     1861 catcacatga gttgaaaacc cctttagcca gtcttagaat tatcctagag aatatgcagc
     1921 ataatattgg agattacaaa gatcatccaa aatatattgc aaagagtata aataagattg
     1981 accagatgag ccacttattg gaagaagtac tggagtcttc taaattccaa gagtggacag
     2041 agtgtcgtga gaccttgact gttaagccag ttttagtaga tattttatca cgttatcaag
     2101 aattagctca ttcaataggt gttacaattg aaaatcaatt gacagatgct accagggtcg
     2161 tcatgagtct tagggcattg gataaggttt tgacaaacct gattagtaat gcaattaaat
     2221 attcagataa aaatgggcgc gtaatcatat ccgagcaaga tggctatctc tctatcaaaa
     2281 atacatgtgc gcctctaagt gaccaagaac tagaacattt atttgatata ttctatcatt
     2341 ctcaaatcgt gacagataag gatgaaagtt ccggtttggg tctttacatt gtgaataata
     2401 ttttagaaag ctatcaaatg gactatagtt ttctccctta tgaacacggt atggaattta
     2461 agattagctt gtagacagat tagtttttta ttaaagttca tatagggtta acataagtgt
     2521 gttattcttt gtgtagataa aagaaaggat actaatatgg tattagcgat tattttagta
     2581 acattcttta ttcgattgat ttttttaaag cgttcgatag agaatgagaa acgaatcctt
     2641 agcaatggcg ggaaagaatt tggagttgag aatacaaaac gattaactat ggctcatatt
     2701 gttttttatc tctcttgctt ggttgaggca atggtgcaca agacaatttt tgatggcatg
     2761 ggcatggttg gtttagtctt gcttattttt tctatgctga tgttaatgtt ggtgattcac
     2821 ttgttgggag atatttggac agtgaagctt atgcttgtca ataatcacaa atatgtagat
     2881 catatcttgt ttaggacagt aaaacatcct aattactttt taaatattct tcctgagttg
     2941 attggcttga ccttgttgag tcatgcttat gtgacttttg ttttagtttt tccagtttat
     3001 gcagttattt tgtatcgacg aatagctgaa gaggaaaagc tattacatga agttataatc
     3061 ccaaatggaa gcataaagag ataaatacaa aattcgattt atatacagtt catattgaag
     3121 tgatatagta aggttaaaga aaaaatatag aaggaaataa acatgtttgc atcaaaaagc
     3181 gaaagaaaag tacattattc aattcgtaaa tttagtattg gagtagctag tgtagtagtt
     3241 gctagcttgt tcttaggagg agtagttcat gcagaagggg ttagaagtgg ggataccccc
     3301 aaggttacat ctagtgggga tgaagtcgag tcgcatctaa aaaaaatatt gagtgagatc
     3361 caaacacaat tagatagaaa acgacatgcc aaaactgtag cgctaattaa caagttgcaa
     3421 gacattaaga gaacgtattc gtataaatta aatgttttag aagagaagcc gaaagctgag
     3481 ttgacgtcaa aaacaaaaga agagttaacc gcagcttttg agcaatttaa aaaagataca
     3541 ttaccaacag aaccaggaaa aaaggtagca gaagctgaga agaaggttgc agaagctgag
     3601 aaaaaagccg aggatcaaaa agaagaagat cgtcgtaact acccaaccaa tacttacaaa
     3661 acgcttgaac ttgaaattgc tgagttcgat gtgaaagtta aagaagcgga gcttgaacta
     3721 ttaaaagaga aagctaagaa acctttaaac gagggcacaa ttaagcaagc aaaagagaaa
     3781 gttgagagta aaaaagctga ggctacaagg ttagaaaaca tcaagacaga tcgtaaaaaa
     3841 gcagaagaag aagctaaacg aaaagcagca gaagaagata aagttaaaga aaaaccagct
     3901 gaacaaccac aaccagcgcc ggctcctcaa ccagaaaa
//
LOCUS       DAVBHS010000158         3894 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVBHS010000158 DAVBHS010000000
VERSION     DAVBHS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.158"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            331..558
                     /locus_tag="VQ651_001849"
     CDS             331..558
                     /locus_tag="VQ651_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754803.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            846..3743
                     /gene="nanA"
                     /locus_tag="VQ651_001850"
     CDS             846..3743
                     /gene="nanA"
                     /locus_tag="VQ651_001850"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HET2754804.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGNRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1328 a          641 c          863 g         1062 t
ORIGIN      
        1 catgcggcta gcttcttagt ttgctctttg attttcattg agtataaaaa cagatgagtt
       61 tctgttttct ttttatggac tataaatgtt cagctgaaac tactttcaag gacattatta
      121 tataaaagaa ttttttgaaa ctaaaatcta ctatattaca ctatattgaa agcgttttaa
      181 aaatgaggta taataaattt actaacgctt ataaaaagtg atagaatcta tctttatgta
      241 tatttaaaga tagattgctg taaaaatagt agtagctatg cgaaataaca gatagagaga
      301 agggattgaa gcttagaaaa ggggaataat atgatattta aggcattcaa gacaaaaaag
      361 cagagaaaaa gacaagttga actacttttg acagtttttt tcgacagttt tctgattgat
      421 ttatttcttc acttatttgg gattgtcccc tttaagctgg ataagattct gattgtgagc
      481 ttgattatat ttcccattat ttctacaagt atttatgctt atgaaaagct atttgaaaaa
      541 gtgttcgata aggattgagc aggaagtatg gtataaatag cataagctga tgtccatcat
      601 ttgcttataa agagatattt tagtttaatt gcagcggtgt cctggtagat aaactagatt
      661 ggcaggagtc tgattggaga aaggagaggg gaaaattggc accaatttga gatagtttgt
      721 ttagttcatt tttgtcattt aaatgaactg tagtaaaaga aagttaataa aagacaaact
      781 aagtgcattt tctggagtaa atgtcttatt tcagaaatcg ggatatagat atagagagga
      841 tcagtatgaa tcggagtgtt caagaacgta agtgtcgtta tagcattagg aaactatcgg
      901 taggagcggt ttctatgatt gtaggagcag tggtatttgg aacgtctcct gttttagctc
      961 aagaaggggc aagtgagcaa cctctggcaa atgaaactca actttcgggg gagagctcaa
     1021 ccctaactga tacagaaaag agccagcctt cttcagagac tgaactttct ggcaataagc
     1081 aagaacaaga aaggaaagat aagcaagaag aaaaaattcc aagagattac tatgcacgag
     1141 atttggaaaa tgtcgaaaca gtgatagaaa aagaagatgt tgaaaccaat gcttcaaatg
     1201 gtcagagagt tgatttatca agtgaactag ataaactaaa gaaacttgaa aacgcaacag
     1261 ttcacatgga gtttaagcca gatgccaagg ctccagcatt ctataatctc ttttctgtgt
     1321 caagtgctac taaaaaagat gagtacttca ctatggcagt ttacaataat actgctactc
     1381 tagaggggcg tggttcggat gggaaacagt tttacaataa ttacaacgat gcacccttaa
     1441 aagttaaacc aggtcagtgg aattctgtga ctttcacagt tgaaaaaccg acagcagaat
     1501 tgccaaatgg tcgagttcgc ctgtatgtta atggtgtttt atctcgaaca agtctgaaat
     1561 ctggcaattt cattaaagat atgccagatg taacgcatgt gcaaatcgga gcaaccaagc
     1621 gtgccaacaa tacggtttgg gggtcaaatc tacagattcg gaatctcact gtgtataatc
     1681 gtgctttaac accagaagag gtacaaaaac gtagtcaact ttttaaacgc tcagatttag
     1741 aaaaaaaact acctgaagga gcggctttaa cagagaaaac ggacatattc gaaagcgggc
     1801 gtaacggtaa accaaataaa gatggaatca agagttatcg tattccagca cttctcaaga
     1861 cagataaagg aactttgatc gcaggtgcag atgaacgccg tctccattcg agtgactggg
     1921 gtgatatcgg tatggtcatc agacgtagtg aagataatgg taaaacttgg ggtaaccgag
     1981 taaccattac caacttacgt gacaatccaa aagcttctga cccatcgatc ggttcaccag
     2041 tgaatatcga tatggtgttg gttcaagatc ctgaaaccaa acgaatcttt tctatctatg
     2101 acatgttccc agaagggaag ggaatctttg gaatgtcttc acaaaaagaa gaagcctaca
     2161 aaaaaatcga tggaaaaacc tatcaaatcc tctatcgtga aggagaaaag ggagcttata
     2221 ccattcgaga aaatggtact gtctatacac cagatggtaa ggcgacagac tatcgcgttg
     2281 ttgtagatcc tgttaaacca gcctatagcg acaagggtga tctatacaag ggtaaccaat
     2341 tactaggcaa tatctacttc acaacaaaca aaacttctcc atttagaatt gccaaggata
     2401 gctatctatg gatgtcctac agtgatgacg acgggaagac atggtcagcg cctcaagata
     2461 ttactccgat ggtcaaagcc gattggatga aattcttggg tgtaggtcct ggaacaggaa
     2521 ttgtacttcg gaatgggcct cacaagggac ggattttgat accggtttat acgactaata
     2581 atgtatctca cttaaatggc tcgcaatctt ctcgtgtcat ctattcagat gatcatggaa
     2641 aaacttggca tgctggagaa gcggtcaacg ataaccgtca ggtagacggt caaaagatcc
     2701 actcttctac gatgaacaat agacgtgcgc aaaatacaga atcaacggtg gtacaactaa
     2761 acaatggaga tgttaaactc tttatgcgtg gtttgactgg agatcttcag gttgctacaa
     2821 gtaaagacgg aggagtgact tgggagaagg atatcaaacg ttatccacag gttaaagatg
     2881 tctatgttca aatgtctgct atccatacga tgcataatgg aaaagaatac attatcctca
     2941 gcaatgcagg tggaccgaat cgtgaaaatg ggatggtcca cttggcacgt gtcgaagaaa
     3001 atggtgagtt gacttggctc aaacacaatc caattcaaaa aggagagttt gcctataatt
     3061 cgctccaaga attaggaaat ggggagtatg gcatcttgta tgaacatact gaaaaaggac
     3121 aaaatgccta taccctatca tttagaaaat ttaattggga atttttgagc aaagatctga
     3181 tttctcctac cgaagcgaaa gtgaagcgaa ctagagagat gggcaaagga gagatgggca
     3241 aaggagttat tggcttggag tttgactcag aagtattggt caacaaggct ccaacccttc
     3301 aattggcaaa tggtaaaaca gcgactttcc taacccagta tgatagcaaa accttgttgt
     3361 ttgcagtaga taaggaagat atcggacagg aaattattgg tatagctaaa ggaagcatcg
     3421 aaagtatgca taatcttcct gtaaatctag caggtgccag agttcctggc ggagtaaatg
     3481 gtagcaaagc agcggtgcat gaagttccag aatttacagg gggagttaat ggtacagagc
     3541 cagctgttca tgaaatcgca gagtataagg gatctgattc gcttgtaact cttactacaa
     3601 aagaagatta tacttacaaa gctcctcttg ctcagcaggc acttcctgaa acaggaaaca
     3661 aggagagtga cctcctagct tcactaggac taacagcttt cttccttggt ctgtttacgc
     3721 tagggaaaaa gagagaacaa taagagaaga attctaaaca tttgattttg taaaaataga
     3781 aggagatagc aggttttcaa gcctgctatc tttttttgat gacattcagg ctgatacgaa
     3841 atcataagag gtctgaaact actttcagag tagtctgttc tataaaatat agta
//
LOCUS       DAVBHS010000159         3880 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVBHS010000159 DAVBHS010000000
VERSION     DAVBHS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.159"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(110..1780)
                     /locus_tag="VQ651_001851"
     CDS             complement(110..1780)
                     /locus_tag="VQ651_001851"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET2754805.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            2010..2699
                     /gene="coaB"
                     /locus_tag="VQ651_001852"
     CDS             2010..2699
                     /gene="coaB"
                     /locus_tag="VQ651_001852"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HET2754806.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            2711..3262
                     /gene="coaC"
                     /locus_tag="VQ651_001853"
     CDS             2711..3262
                     /gene="coaC"
                     /locus_tag="VQ651_001853"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HET2754807.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            3246..3809
                     /locus_tag="VQ651_001854"
     CDS             3246..3809
                     /locus_tag="VQ651_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2754808.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
BASE COUNT         1161 a          873 c          697 g         1149 t
ORIGIN      
        1 ttgaagagat tttcgaagag tataaatctt attgatgaac tgcttgcagt ctgagaaaaa
       61 atgagcttgg atattatttc caaactcact taaagtcaat ttcaatccac tagaacaagc
      121 ctagtacagt tccatcgctt tcaacatcca tgttgagagc tgctggacgt tttggaagac
      181 ctggcatggt cataacatca ccagttaagg caacgatgaa gcctgcacct aattttggta
      241 ccaattcacg aatggtaatt tcaaagtttt ctggtgctcc aagcgcattt ggattgtctg
      301 agaaactgta ttgagtttta gccatacaga ttggcaattt gtcccaaccg ttttgaacga
      361 tttgagcaat ttgtgtttga gctttcttct caaagttcac tttgctacca cgatagattt
      421 cagtgacaat tttttcaatc ttttcttgga cagaaagggc attatcgtac aaacgtttat
      481 agttagctgg attttcagca attgtcttaa caactgtttc ggcaagtgct actccacctt
      541 ctgctccatc agcccagaca ctagccaatt caactggtac atcgattgag gcacagagtt
      601 cttttaagac tgcaatttca gcttctgtat cagatacaaa ttcgttaata gctacaactg
      661 ctggaatacc gaacttacgg atattttcaa cgtggcgttt caagttagca aaacctgcac
      721 gaactgcctc tacattttct tcagtcagag cgtctttagc cacaccacca ttcatcttaa
      781 gggcacgaag ggttgcgaca atgacaactg catctggaga tgttggcaag tttggtgtct
      841 tgatatcaag gaatttctca gcaccaaggt ccgcaccaaa accagcttca gtaacagtgt
      901 aatcagccaa gtgaagggct gttgtcgtcg ccaaaacaga gttacagcca tgagcgatat
      961 tggcaaatgg accaccgtgt acaaaggcag gtgtaccgta aattgtctga accaagtttg
     1021 gcttaatagc atccttcaaa atcaaagcca aggcaccctc aacctgcaaa tcacctacag
     1081 aaacaggcgt acggtcatag cgataaccaa taacgatatt cgccaaacga cgtttcaagt
     1141 cctcgatgtc cgttgccaag caaagaattg ccatgatttc tgaagcaact gtaatatcaa
     1201 aaccatcctc acgtggaata ccgtttagag gaccaccaag accaacagtc acatggcgga
     1261 gcgcacggtc gttcaagtcc acaacgcgtt tccagaggat acgacgttga tcaatttcca
     1321 gctcattccc ttggtgcaag tggttgtcaa tcaaggcaga aagggcattg ttggcagttg
     1381 taatagcatg catatctcca gtaaagtgga ggttgatgtc ttccattggc agaacttgtg
     1441 catacccacc accagcagca ccacccttga tccccatgac tggaccaaga gacggttcgc
     1501 ggatagcaat catggttttc ttgccaatct tgttcaaggc atccgcaaga ccaatggtaa
     1561 gcgtcgactt tccttcacct gcaggtgttg ggttgatggc agtaaccaag atcaatttac
     1621 cgactggatt gctctcaact gcacgaattt tatcaaagct gagtttagcc ttgtactttc
     1681 cgtacaactc caaatcgtca taagaaatac caagtttctc tacaacatca acaattggct
     1741 tcaactcaat actctgtgcg atttcaatat ctgttttcat tcaaaattcc tctaacctct
     1801 tatatgataa ttcattatat cacaaaacaa gatttttaac atcctaaaac tctctaaacg
     1861 ttcgtaaata tctctgtttt taaggctttt agagtccttt cttaaatttt atatggcttt
     1921 atagtttgaa actatagtaa atcttcgttt ttaccaaaaa tttatcactt tcattttact
     1981 taccgcttat ttttgtgtac aatagtgcta tgaaaatttt agttacatcg ggcggtacca
     2041 gtgaagctat cgatagcgtc cgctctatca ctaaccattc tacaggtcac ttggggaaaa
     2101 ttatcacaga gactttgctt tctgcagggc atgaagtttg tttaattacg acaaaacgag
     2161 ctctgaagcc agagcctcat cctaacctaa gtattcgaga aattaccaat accaaggacc
     2221 ttctaataga aatgcaagaa cgtgttcagg attatcaggt cttgatccac tcaatggctg
     2281 tttctgacta cactcctgtt tatatgacag ggcttgagga agttcaggct agctccaatc
     2341 taaaagaatt tttaagcaag cagaatcatc aggccaagat ttcttcaact gatgaggttc
     2401 aggttttgtt ccttaaaaag acacccaaaa tcatatccct agtcaaggaa tggaatccca
     2461 ctattcatct gattggtttc aaactgctgg ttgatgttac cgaagatcat ctggttgaca
     2521 ttgcacgaaa aagtcttatc aagaatcaag cagatttaat catcgcgaat gacctgactc
     2581 aaatttcagc agatcagcac cgagctatat ttgttgagaa aaatcagctt caaacagtcc
     2641 agactaaaga agaaattgca gaactcctcc ttgaaaaaat tcaagcctat cattcttaga
     2701 aaggaaaact atggcaaaca ttctcttggc tgtaacgggt tcaatcgcct cttataagtc
     2761 ggcagattta gtcagttctc taaaaaaaca aggccatcaa gtcactgtct taatgactca
     2821 ggctgctaca gagtttatcc aacctttgac actacaggta ctctcacaga atcctgtcca
     2881 cttggatgtc atgaaggaac cctatcctga tcaggtcaat catatcgaac ttggaaaaaa
     2941 agcagattta tttatcgtgg tacctgcaac tgctaacact attgcaaaac tagctcacgg
     3001 atttgcggac aacatggtaa ccagtacagc tctagcccta ccaagtcata ttcccaaact
     3061 aatagctcct gctatgaata caaaaatgta tgaccatcca gtaactcaga ataatctgaa
     3121 aacattagaa acctacggct atcagctgat tgctcctaag gaatccctac tagcttgtgg
     3181 agaccacgga cgaggagctt tagctgacct cacaattatt ttagaaagaa taaaggaaac
     3241 tatcgatgaa aaaacgctct aatattgcac ccattgctat cttttttgct accatgctcg
     3301 tgatacactt tctgagctca cttatcttta acctttttcc atttccaatc aaaccgacca
     3361 ttgttcatat tcctgtcatt attgccagca ttatttatgg tccacgagtt ggggttacac
     3421 ttggattttt gatgggatta cttagcttga cggttaacac gattacgatt ctaccgacaa
     3481 gctacctctt ctctcccttc gtaccaaacg gaaacatcta ctcagctatc attgccatcg
     3541 tcccacgtat tttgattggt ttaactcctt acttagtcta taaactgatg aaaaacaaga
     3601 ctggtctgat tttagctgga gcccttggtt ccttgacaaa tactatcttt gtccttggag
     3661 gaatcttctt cctatttgga aatgtttata atggaaatat ccaacttctt ctggcaaccg
     3721 ttatctcaac aaattcaatt gctgaattgg tcatttctgc aattctaacc ctagccattg
     3781 ttccacgact acaaaccttg aaaaaataaa aacaatctcc gctttcaaac ctgaaggtgg
     3841 agattttgct ctatacagtt tctttttaat gaaatcttta
//
LOCUS       DAVBHS010000160         3872 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVBHS010000160 DAVBHS010000000
VERSION     DAVBHS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.160"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            98..1357
                     /locus_tag="VQ651_001855"
     CDS             98..1357
                     /locus_tag="VQ651_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HET2754809.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQIQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    1433..1568
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1607..2107
                     /locus_tag="VQ651_001856"
     CDS             1607..2107
                     /locus_tag="VQ651_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET2754810.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            2183..2551
                     /gene="rplL"
                     /locus_tag="VQ651_001857"
     CDS             2183..2551
                     /gene="rplL"
                     /locus_tag="VQ651_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET2754811.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKTKLEEAGASVTLK"
     gene            2738..2908
                     /locus_tag="VQ651_001858"
     CDS             2738..2908
                     /locus_tag="VQ651_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754812.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            2908..3156
                     /locus_tag="VQ651_001859"
                     /pseudo
     CDS             2908..3156
                     /locus_tag="VQ651_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            3170..3510
                     /locus_tag="VQ651_001860"
                     /pseudo
     CDS             3170..3510
                     /locus_tag="VQ651_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3503..>3872
                     /locus_tag="VQ651_001861"
     CDS             3503..>3872
                     /locus_tag="VQ651_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET2754813.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHY"
BASE COUNT         1239 a          691 c          826 g         1116 t
ORIGIN      
        1 agatagaact gacgaagtca gtaaccacac ctacggcaaa gtgaatctga agtggtttga
       61 agagagtaca acttgtcttt tagaaaagga gcctataatg aaagtctttc agcatgtaaa
      121 tatcgtgact tgtgatcaag atttccatgt ttatcttgat ggaatcttag cagtcaagga
      181 ttctcaaatc gtctatgtcg gtcaagataa gccagcgttt ttagagcaag ctgagcagat
      241 tatagactat cagggagctt ggattatgcc tggtttggtc aattgtcaca cccattctgc
      301 aatgacaggt ctgagaggga tccgagatga cagcaatctc catgaatggc tcaatgacta
      361 tatctggcca gcagaatctg agtttactcc cgacatgact accaatgcgg tcaaagaagc
      421 cctgacagag atgctccagt caggaacaac aacctttaac gatatgtata atcccaatgg
      481 tgtggatatc cagcaaattt atcaggtggt gaaaacttcc aagatgcgtt gttatttttc
      541 tccgactctc ttttcttcag agacagaaac aactgctgag actataagca gaactcgatc
      601 catcatagac gaaatcttaa aatataaaaa tccaaatttc aaggttatgg tagcacctca
      661 ttctccgtat agctgcagta gagacttgct ggaagcgagt ttggaaatgg caaaagagct
      721 aaatattccg ctccatgtcc atgtggcgga gaccaaggag gagtcaggaa ttatcctcaa
      781 acgatacggc aaacgccccc ttgcttttct tgaagaactg ggttatttag atcatccgtc
      841 cgtatttgct cacggggtcg aattaaacga gagagaaatt gaacgcttgg catcttctca
      901 agtggctatc gcccacaatc ctatcagtaa cctcaaactg gcatcaggaa ttgctccaat
      961 tatccagatc caaaaagcgg gagtagcagt cggaattgcg actgactcgg ttgcttccaa
     1021 taacaatcta gatatgtttg aggaaggaag gactgcagct cttcttcaga agatgaaaag
     1081 tggggatgcc agccagtttc caatcgaaac agctctcaag gtactgacaa tcgaaggggc
     1141 taaggtcctt ggaatggaaa atcagatagg aagtctggaa gtcggcaagc aagcagattt
     1201 tctggtcatt caaccacaag ggaaaattca tctccaacct caggaaaata tgctgtctca
     1261 cctggtttat gcagttaaat ctagtgatgt agatgatgtt tatatcgccg gagaacaggt
     1321 tgttaagcaa ggtcaagtcc tgacagtaga actttaaaag aaaaatcacg aaaaatttta
     1381 aaaaaagttt gcaaaaatct tgcattcttt ttttgactat gctatactta tatacggttt
     1441 aaaaaaactg cctaagacag taggggagct cgactcataa atatcctacc gaggacaaga
     1501 cgtatcatgt aaaaagaagc gtattgtact ttcgtgtcta ggtttgggcg cgtttttctt
     1561 tttgaaaaat tccccaagca aaataattac ggaggtgaac acactaatga gtgaagcaat
     1621 tattgctaaa aaagcggaac tagttgacgt agtagctgaa aaaatgaaag ctgctgcatc
     1681 tatcgtcgtt gtagacgctc gtggtttgac agttgagcaa gatacagttc ttcgtcgcga
     1741 gcttcgtgga agcgaagttg agtataaagt tattaaaaac tcaatcttgc gtcgtgcagc
     1801 tgaaaaagct ggtcttgaag atcttgcatc tgtatttgtt ggaccatctg cagtagcatt
     1861 ttctaatgaa gatgttatcg caccagcgaa aatcttgaac gacttttcta aaaacgctga
     1921 agcacttgaa attaaaggtg gtgcaatcga aggcgctgtc gcatctaaag aagagattct
     1981 tgcacttgca actcttccaa accgcgaagg acttctttct atgctccttt ctgtacttca
     2041 agcgccagtg cgcaacgttg ctcttgcagt caaagcggtt gcagaaagca aagaagacgc
     2101 ggcttaatct taagctacac agcgtagcct agctacgaaa aaaactatta taaaatttaa
     2161 aacttatttg gaggaaataa caatggcatt gaacattgaa aacattattg ctgaaattaa
     2221 agaagcttca atccttgaat tgaacgacct tgtaaaagct atcgaagaag aatttggtgt
     2281 aactgcagct gctcctgtag ctgttgctgc agctgatgca gctgatgctg gtgctgctaa
     2341 agattcattc gacgttgaat tgacatctgc aggcgacaaa aaagttggcg ttatcaaagt
     2401 tgtacgtgaa atcactggtc ttggtcttaa agaagctaaa gaacttgttg acggtgcacc
     2461 agcacttgtt aaagaaggcg ttgcaactgc agaagctgaa gaaatcaaaa ctaaattgga
     2521 agaagctgga gcttcagtta ctcttaaata atagagtatc gcaatttgat tgcggaatcc
     2581 ctatttacca gtctttgaga gcgataagcg attgatagaa actgataaat tgatttggtt
     2641 tcctgccaaa aattctttgg caggattttt gttttaataa actttcgggg gtagacacta
     2701 gattgtctac cccttttttt gtgtttcgga ggtaattatg caaaaggaag gacagtcatc
     2761 acgtcaattc gtgaagagtc atctcatgac tatcatggga gttgatattg taaaagcaac
     2821 tcagttagtt gatgaaatgg aacaagtagg tttaatccga tttgatgaat ttggaaatgt
     2881 tggaatttta gtcttggagg gacaataatg aagtgtatta ccgctaatca atatcagaca
     2941 tcagagcgtt attataagct ccccaaactt ttatttgaga gtgaacggta taagaatatg
     3001 aaactggagg ttaaggtggt ttactcagtt ttaaaagatc ggttggagtt gtctttgagc
     3061 aaaggttgga ttgatgagga tggggctatt tatttgattt attccaattc aaatttgatg
     3121 gcacttttag gctgttcaaa gtcaaaatta ctctccatgt gagtttgaag tgacattttt
     3181 agatgattac cataaaaaac ataactaccc actattttac gaatcctatc ttcaaaacgt
     3241 tatggaattc cttgaaagtc aagacataaa gaatggggtt gatgcctttg tagatgatca
     3301 tcaaaatctc gtttttgttt tatatggaca aggctatcga gccgagggaa aagagggaat
     3361 acttacaacc caagtaactg taaaagctta tgatgaagac aagaaaccga ttaacttcgc
     3421 aaatttatta gattccttaa tttattagat tccttaatcg tgtcagaata tcaaatggaa
     3481 ccgaatcttt gggaggtctc ccatgattga tctctatcta agtaaaaata gacaaagaaa
     3541 tcaacttctt ttagatttct tccaaaacta tggcatagag gtatcttgtc attcagtttc
     3601 tgaaatgaca aaggacaaat taattgagat gatgagctat tcttcagatt gttttgagtt
     3661 tttatctccc aatttattac gatttaagaa ccgtgacaat ctaagattaa cggatttcgt
     3721 tgagataatt ttaaaaaatc ctgaactaac catcagactt cctcttgcgg tttcaaataa
     3781 gcgagtttat ccaagtctga atttggaaga ggctagagct ctattgccaa gagatacgaa
     3841 acaattgatt tacatggcgc aaacacatta tt
//
LOCUS       DAVBHS010000161         3754 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVBHS010000161 DAVBHS010000000
VERSION     DAVBHS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.161"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            172..1563
                     /locus_tag="VQ651_001862"
     CDS             172..1563
                     /locus_tag="VQ651_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET2754814.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIDQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            1677..2519
                     /locus_tag="VQ651_001863"
     CDS             1677..2519
                     /locus_tag="VQ651_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET2754815.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            2792..3553
                     /locus_tag="VQ651_001864"
     CDS             2792..3553
                     /locus_tag="VQ651_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2754816.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
BASE COUNT         1199 a          606 c          832 g         1117 t
ORIGIN      
        1 aggtctacta ttctagcttc aatctactag aaattccata gatagaaaac tacataatct
       61 ctacagatac ggatgttgga gttgatgtaa gatgctttgg cttgctagag gaattgtgga
      121 ttgccaaatt gtatcattga aattattgct caaatttgtt atgatataaa tatgaataaa
      181 agtagactag gacgtggcag acacgggaaa acgagacatg tattattggc tttgattggt
      241 attttagcaa tttctatttg cctattaggc ggatttattg cttttaagat ctaccagcaa
      301 aaaagttttg agcaaaagat tgaatcgctc aaaaaagaga aagatgatca attgagtgag
      361 ggaaatcaga aggagcattt tcgtcagggg caagccgaag tgattgccta ttatcctctc
      421 caaggggaga aagtgatttc ctctgttagg gagctgataa atcaagatgt taaggacaag
      481 ctagaaagta aggacaatct tgttttctac tatacagagc aagaagagtc aggtttaaag
      541 ggagtcgtta atcgtaatgt gaccaaacaa atctatgatt tagttgcttt taagattgaa
      601 gagactgaaa agaccagtct aggaaaggtt cacttaacag aagatgggca accttttaca
      661 cttgaccaac tgttttcaga tgctagtaag gctaaggaac agctgataaa agagttgacc
      721 tccttcatag aggataaaaa aatagatcaa gaccagagtg agcagattgt aaaaaacttc
      781 tctgaccaag acttgtctgc atggaatttt gattacaagg atagtcagat tatcctttat
      841 ccaagtcctg tggttgaaaa tttagaagag atagccttgc cagtatctgc tttctttgat
      901 gttatccaat cttcgtactt actcgaaaaa gatgcggcct tgtaccaatc ttactttgat
      961 aagaaacatc aaaaagttgt cgctctaacc tttgatgatg gtccaaatcc agcaacgacc
     1021 ccgcaggtat tagagaccct agctaaatat gatattaaag cgactttctt tgtgcttggg
     1081 aaaaatgttt ctgggaatga ggacttggtg aagaggataa aatctgaagg tcatgttgtt
     1141 ggaaaccata gttggagcca tccgattctc tcgcaactct ctcttgatga agctaaaaag
     1201 cagattactg atactgagga tgtgctaact aaagtgctgg gttctagttc taaactcatg
     1261 cgtccacctt atggtgctat tacagatgat attcgcaata gcttggattt gagctttatc
     1321 atgtgggatg tggatagtct ggactggaag agtaaaaatg aagcatctat tttgacagaa
     1381 attcagcatc aagtagctaa cggctctatc gttttgatgc atgatattca cagtccgaca
     1441 gtcaatgcct tgccaagggt cattgagtat ttgaaaaatc aaggttatac ctttgtgacc
     1501 ataccagaga tgctcaatac tcgcctaaaa gctcatgagc tgtactatag tcgtgatgaa
     1561 taagcaagaa aaaataggtc tgttagatat ttgacagact tattttttac agaatatagt
     1621 actacttaaa aaatgtttta tgctataatt gatgaataaa atagaaggag aagcatatga
     1681 atacctatca attaaataat ggagtagaaa ttccagtatt gggatttgga acttttaagg
     1741 ctaaggatgg agaagaagcc tatcgtgcag tgttagaagc cttgaaggct ggttatcgtc
     1801 atattgatac ggcggcgatt tatcagaatg aagaaagtgt tggtcaagca atcaaagata
     1861 gcggagttcc acgtgaagaa atgttcgtaa ctaccaagct ttggaatagt cagcaaacct
     1921 atgagcaaac tcgtcaagct ttggaaaaat ctatagaaaa actgggcttg gattatttgg
     1981 atttgtattt gattcattgg ccgaacccaa aaccgctcag agaaaatgac gcatggaaaa
     2041 ctcgcaatgc ggaagtttgg agagcgatgg aagacctcta tcaagaaggg aaaatccgtg
     2101 ctatcggcgt tagcaatttt cttccccatc atttggatgc cttgcttgaa actgcaacta
     2161 tcgttcctgc ggtcaatcaa gttcgcttgg cgccaggtgt gtatcaagaa caagtcgtag
     2221 cttactgtcg tgaaaaggga attttattgg aagcttgggg gccttttggt cagggagaac
     2281 tatttgatag caagcaagtg caagaaatcg cagcaaatca cggaaaatcg attgctcaga
     2341 tagccttggc ctggagcttg gcagaaggat ttttaccact tccaaaatct gtcacaacct
     2401 ctcgtattca agctaatctt gattgctttg gaattgaact gagtcatgag gagagagaaa
     2461 ccttaaaaac gattgctgtt caatcgggtg ctccacgagt tgatgatgtg gatttctaga
     2521 aaatcataaa aagaattgta cattattcta atttttgata taatagtcag caggaaagaa
     2581 agtcttatgg cgttcttcaa gcgagcttgg gatagtggga gccaagtagg gcaaaataat
     2641 gagctggcgc tttctgtagt attttcaaaa acaatgaagt aataaattag ggtggaaccg
     2701 cgtttctgac gcccctaggt taaatcaacc taggattgtc ggacgcggtt cttttgctta
     2761 ttcagtctat tgtgtgaaag aaaggagagc cgtggacaac ctttatcttg taaaagacga
     2821 tagtcaacta gctacatttc gtgattttgt agtaagaaat actgaaaagt tgaaagatta
     2881 tcaatctttt ttaaagaatg aacttgcagt ctgtgattta ccgcaagccg ttatttggtc
     2941 agattttaat gctgctacac agattattag ggaaagcgct gttccagcct atacaaataa
     3001 tagacgaatg gttatggcgc ctgatttagc tgtttggaaa gagttgtatt tgtatcaatt
     3061 gatggactac gagtgttctc agcaaactca agcaatagaa agtcactatc attctttatc
     3121 tgaaaatttc ctcttacaga ttgtaggaca tgagttagct cattggtcgg agcatttttt
     3181 agatgatttt gatggttatg actcttatat ctggttcgaa gaggggatgg ttgaatatat
     3241 tagtcgcaag tatttcttga cagaagagga atttcaagcg gaaaaaattt gtaatcaatc
     3301 tctcgtagaa ctttttcaga agaagtatgg ttggcattca ttgaatgatt ttggttcttc
     3361 gacttatgat aagaactatg caagtatttt ttatgaatac tggcgcagct ttttgacagt
     3421 agataagttg gtagaaaatt taggtagtgt acaagcggtc ttagattctt atcatttatg
     3481 ggcaaataca gaaaaaactt ttcccttgtt agattggttt gttcagcaga aattaattga
     3541 aaaagaaata taaaaactaa aggagtaaac aatgtctaag aaattgacat ttcactgcgt
     3601 cagtggcaga aacctcctta cagtcggact gccccacgct cagcactaga gtgcctgagc
     3661 tagacgcagt actaactcgt cttgcctcgt atgatcgacg aggcagactc atgtcgcaag
     3721 taattatttt ttattaagga gtaaacaatg tcta
//
LOCUS       DAVBHS010000162         3720 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVBHS010000162 DAVBHS010000000
VERSION     DAVBHS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.162"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(1..801)
                     /locus_tag="VQ651_001865"
     CDS             complement(1..801)
                     /locus_tag="VQ651_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2754817.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            972..1577
                     /locus_tag="VQ651_001866"
     CDS             972..1577
                     /locus_tag="VQ651_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET2754818.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDLKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            1590..2990
                     /gene="pepV"
                     /locus_tag="VQ651_001867"
     CDS             1590..2990
                     /gene="pepV"
                     /locus_tag="VQ651_001867"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HET2754819.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLTDLQAKLDAFVAEHKLRGELQEEAGQYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            3044..3622
                     /locus_tag="VQ651_001868"
     CDS             3044..3622
                     /locus_tag="VQ651_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET2754820.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPCT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
BASE COUNT         1117 a          759 c          822 g         1022 t
ORIGIN      
        1 ttattgtaac tgagattgat ctgggaggta agaaccacct agataggtat tgctgagttt
       61 ttcaagggtt ccgtcttgat agagttcttt gagtgcttta tcaaattgct ctttaaactc
      121 tttttggtcg cttgagaaaa tgatataatt gctggggcta tctgcagaag gtaaatcaac
      181 gactgagagg tctaaaccac ggtccttgat aatcttttga acggatacct tgtcaaaaac
      241 taggaaatca aactctccgt tagcaaggtc taggattcgt ttaccaatat cctcaccaga
      301 aaaattaatt gtagcgggat tatcagtgtg tttctgattc cagttattga tgaattgagc
      361 gttagaagtt ccggtatcct cttgtgttgt tttaccagcg atctggtcaa gagaagtcaa
      421 aggatttttc ttgttgctga caaggacgag gggattgttg gaaatcggaa gcgagtaaag
      481 gtatttttca gcacgctctt ttgtgtaact caaattattg gccgcagcct gatagtgacc
      541 agaatcaagt cctgggaaga tactttccca ggcggttctt tgaaattgaa tctcgtagtc
      601 gctgagtttt tcatctactg cctttagaac ttcgatatcg aagcctgtca gattgccttt
      661 atcttcgtag tcaaatggtg gcacgtcgcc agctgtagca aggacgattg tcttttgagc
      721 gctagtctct ttgggtgtag cttgattccc acaggcaacc aaaaatggta ggatagctag
      781 taataggcta aattttttca tactgtctcc attcaaatgt aaagtacttg ctagtttatc
      841 agaaactttc ctgataaacc aatatatatc ttttatgtct gtgatagaaa aacttaatca
      901 tgaaaaatcc ctgcaagctc tttcaaatta tggtagaatg gaagcagtag aattagaaaa
      961 ggaaatcgat tatgaaattt cttgaattaa ataaaaaacg tcatgcgacc aagcatttca
     1021 ctgataagcc tgttgatcta aaagatgtgc gtacggctat cgaaattgca accttggcgc
     1081 caagcgccca caacagccag ccttggaaat ttgtagttgt tcgtgagaaa aatgctgaac
     1141 tggcaaagtt ggcttatggt tcaaactttg agcaggtatc atcagcacct gtaaccattg
     1201 ccctatttac agatacagac cttgccaaac gcgctcgtaa gattgcccga gttggtggtg
     1261 ctaacaattt ctcagaagaa caacttcaat acttcatgaa aaatttgcca gctgaatttg
     1321 cacgctacaa tgaacaacaa gtcagcgact acctagcact caatgcaggt ttggttgcta
     1381 tgaacttggt tctcgctctt acagaccaag gaattggttc caatattatc cttggttttg
     1441 acaaatcaaa agtcaatgag gttttggaaa ttgaagaccg tttccgccca gaactcttga
     1501 tcacagtggg ttacacagac gacaaattgg aaccaagcta ccgcttgcca gtagatgaaa
     1561 tcatcgaaaa aagataggaa gaagaaaaaa tgatagcaat tgattttaca gcagaagtag
     1621 aaaaacgcaa agaagacctc ttggctgact tgtttagcct tttggaaatc aactcagaac
     1681 gtgatgatag caaggctgat gcccaacatc catttggacc tggtccagta aaagccttgg
     1741 aaaaattcct tgaaatcgca gaccgtgatg gttatccaac taaaaatgtt gataactatg
     1801 caggacattt tgagtttggt gatggagaag aagttctcgg aatctttgcc catatggacg
     1861 tggttccagc tggtagcggt tgggacacag acccttacac accaactatc aaagatggtc
     1921 gcctttatgc gcgtggtgct tcagatgaca agggacctac aacagcttgc tactatggtt
     1981 tgaaaatcat caaagaattg ggccttccaa cttctaagaa agttcgcttc atcgttggaa
     2041 cagacgaaga atcaggctgg gcagatatgg actactactt cgagcacgta ggactcgcca
     2101 agccagactt tggtttctca ccagatgctg aatttccaat catcaatggt gaaaaaggaa
     2161 acatcacgga atacctccac tttgcaggtg aaaatacagg tgcagcccgt cttcacagct
     2221 ttacaggtgg tttacgtgaa aatatggtac cagaatcagc aacagcagtc gtttcaggtg
     2281 acttgacaga cttgcaagct aaactagatg cctttgttgc agaacacaaa cttagaggag
     2341 aactccaaga agaagctggc caatacaagg tgacgatcat tggtaaatca gcccacggtg
     2401 ctatgcctgc ttcaggtgtc aatggcgcaa cttaccttgc cctcttcctc agccagtttg
     2461 gctttgctgg tccagccaaa gactaccttg acatcgcagg taaaattctc ttgaacgatc
     2521 atgagggtga aaatctcaag attgctcatg tggatgaaaa gatgggtgct ctttctatga
     2581 atgccggcgt cttccgcttc gatgaaacaa gtgctgataa taccattgcc cttaacatcc
     2641 gctatccaaa aggaacaagt ccagaacaaa tcaagtcaat cctagaaaac ttgccagttg
     2701 cttctgtcag tctatcagaa catggtcata cccctcacta tgtgccaatg gaagatccac
     2761 ttgtgcaaac cttgttgaat gtctatgaaa aacaaacagg ccttaaaggt catgaacaag
     2821 tcatcggtgg tggaaccttt ggtcgcttgt tagagcgcgg agttgcctat ggtgctatgt
     2881 tcccagactc aattgatacc atgcaccaag ccaatgaatt tattgccttg gacgatctct
     2941 tccgagcagc agcaatttat gccgaagcta tttacgaatt gatcaaataa aacgatagaa
     3001 gtctgagatc ttatgcttgg acttcttttt ggagggaaag tagatgtctc aaatcgaaag
     3061 aatcaaacag gctatcatgg cggattcgca gaatgccagc tatacagagc gtggcattga
     3121 gcctctcttt gcagcgccaa aaactgctcg catcaatatc atcggtcagg ctccgggact
     3181 taaaactcaa gaagcaggcc tttactggaa agataaaagt ggtgaccgtt tgcgggactg
     3241 gctaggtgtg gatgaagata ccttttacaa ttcaggttat tttgctgttt tgcctatgga
     3301 tttctacttt ccaggacatg gcaagtcggg tgatcttccg ccttgtacag gttttgcaga
     3361 aaaatggcat ccgcagctct tacaggaatt gcctgatatt cagttaaccc tcttgattgg
     3421 gcaatatgcc caagcctact atttacagga gaaaatcagt gggaaggtaa cggagagggt
     3481 gaaacactat aaagactatc tgccagccta ttttccgcta gttcacccat caccacgaaa
     3541 tcaaatctgg atggccaaaa atccttggtt tgaggcagaa gtagtgccag atttgaaaaa
     3601 aagaattaaa accattttat agtcaatgaa aatcaaagag caaactagga agctagtcgt
     3661 aggctgctca aagtacagct ttgaagttgc agataaaact gacgaagtcg gtaacatacg
//
LOCUS       DAVBHS010000163         3714 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVBHS010000163 DAVBHS010000000
VERSION     DAVBHS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.163"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            88..1005
                     /gene="glyQ"
                     /locus_tag="VQ651_001869"
     CDS             88..1005
                     /gene="glyQ"
                     /locus_tag="VQ651_001869"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET2754821.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            1265..3301
                     /locus_tag="VQ651_001870"
     CDS             1265..3301
                     /locus_tag="VQ651_001870"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET2754822.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSELFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            3343..3600
                     /locus_tag="VQ651_001871"
     CDS             3343..3600
                     /locus_tag="VQ651_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HET2754823.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
BASE COUNT         1144 a          693 c          840 g         1037 t
ORIGIN      
        1 tagacgcagt actaactcgt cttgcctcgt ataatcgacg aggcagactc gtgtcgcaag
       61 aaatcatttt ttattaagga gtaaacaatg tctaagaaat taacatttca agaaattatt
      121 ttgactttgc aacaattttg gaatgaccaa ggttgtatgc ttatgcaggc ttatgacaat
      181 gaaaaaggtg cggggacaat gagtccttac actttccttc gtgctatcgg acctgagcca
      241 tggaatgcag cttatgtaga gccatcacgt cgtcctgctg acggtcgtta tggggaaaac
      301 cctaaccgtc tctaccaaca ccaccaattc caggtggtca tgaagccttc tccatcaaat
      361 atccaagaac tttaccttga gtctttggaa aaattgggaa tcaatccttt ggagcacgat
      421 attcgttttg ttgaggacaa ctgggaaaac ccatcaactg gttcagctgg tcttggttgg
      481 gaagtttggc ttgacggaat ggaaatcact cagttcactt atttccaaca agtcggtgga
      541 ttggcaactg gccctgtgac tgcggaagtt acctatggtt tggagcgctt ggcttcttac
      601 attcaagaag tagactctgt ctatgatatc gagtgggctg atggtgtaaa atacggagaa
      661 atctttatcc agcctgagta tgagcactca aaatattcat ttgaaatttc ggaccaagaa
      721 atgttgcttg aaaactttga taagtttgaa aaagaagctg gtcgtgcatt agaagaaggc
      781 ttggtacacc ctgcctatga ctatgttctc aaatgttcac atacctttaa tctgcttgac
      841 gcgcgtggtg ccgtatctgt aacagagcgt gcaggctata tcgctcgtat ccgtaacttg
      901 gcccgtgttg tagccaaaac ctttgttgca gaacgcaaac gcctaggtta cccacttttg
      961 gatgaagaaa cacgagttaa acttttagca gaagacgcag aatagtgagt aatactgtac
     1021 tctaaaatcg ttagaggcaa gtcgaagact tgctagaggg atatgcaccg ccgtactgtt
     1081 atgtggggat ttttgaaacc ttaggttcaa aaatcagtca gctaaatcca ctttgatgag
     1141 attgttggaa atcttgataa atttagcatt ggatttccat acagactcac agagttagtt
     1201 agcgacgtcc ccagtaccta aggtgcatat caaaaaagat tgaagaaaat gtaaaggaaa
     1261 aaacatgaca aaaaacttat tagtagaact cggtcttgaa gaattaccag cctatgttgt
     1321 cacaccaagt gaaaaacaac taggcgaaaa aatggcagcc ttcctcaagg aaaaccgcct
     1381 gtcttttgaa gccattcaaa ccttctcaac accacgtcgt ttggctgttc gtgtgactgg
     1441 tttatcagac aaacagtctg atttgacaga agatttcaag ggaccagcaa agaaaattgc
     1501 cttggatagt gatggaaact ttaccaaagc agctcaagga tttgtccgtg gaaaaggctt
     1561 gactgttgaa gatatcgaat tccgtgaaat caagggtgaa gaatatgtct atgtcactaa
     1621 ggaagaagtt ggtcaagcag ttgaagccat tgttcctggt gttgtagatg tcttgaagtc
     1681 attgactttc cctgtcagca tgcactgggc tggaaatagc tttgaataca ttcgtcctgt
     1741 ccacacttta actgttctct tggatgagca agagtttgac ttggatttcc ttgatatcaa
     1801 gggaagtcgt gtgagccgtg gccatcgttt cttggggaaa gaaaccaaaa ttcagtcagc
     1861 attgagctat gaagaagacc ttcgtaagca gtttgtaatc gcagatccat gtgaacgtga
     1921 gcaaatgatt gttgaccaaa tcaaggaaat tgaggcaaaa catggtgtac gtatcgaaat
     1981 tgatgcggat ttgctgaatg aagtcttgaa tttggttgaa tacccaactg ccttcatggg
     2041 aagttttgat gctaaatacc ttgaagttcc agaagaagtc ttggtgactt ctatgaagga
     2101 acaccagcgt tactttgttg ttcgtgatca agatggaaaa ctcttgccaa acttcatttc
     2161 tgttcgtaac ggaaacgcag agcgtttgaa aaatgtcatt aaaggaaatg aaaaagtctt
     2221 ggtagcccgc ttggaagacg gagaattctt ctggcgtgaa gaccaaaaat tggtgatttc
     2281 agatcttgtt gaaaaattaa acaatgtcac cttccatgag aagattggtt cccttcgtga
     2341 acacatgatt cgtacgggtc aaatcactgt acttttggca gaaaaagctg gtttgtcagt
     2401 ggatgaaaca gttgaccttg ctcgtgcagc agccatttac aagtttgact tattgacagg
     2461 tatggttggt gaatttgacg aactccaagg aattatgggt gaaaaataca cccttcttgc
     2521 tggtgaaact ccagcggtgg cagctgctat tcgtgaacac tacatgccta catcagctga
     2581 aggagaactt ccagagagca aggtcggcgc agttctagcc attgcagaca aattggatac
     2641 gattttgagt ttcttctcag taggattgat tccatcaggt tctaatgacc cttatgccct
     2701 tcgtcgtgca actcaaggtg tggttcgtat cttggatgcc tttggttggc acattgctat
     2761 ggatgagctg attgatagcc tttatgcatt gaaatttgac agtttgactt atgaaaataa
     2821 agcagaggtt atggacttta tcaaggctcg tgttgataag atgatgggct ctactccaaa
     2881 agatatcaag gaagcagttc ttgcaggttc aaactttgtt gtggcagata tgttggaagc
     2941 agcaagtgct ctcgtagaag taagcaagga agaagatttt aaaccatctg ttgaatcact
     3001 ttctcgtgcc tttaacctgg ctgagaaggc agaaggggtt gctacagttg attcagaact
     3061 atttgagaat gaccaagaaa aagctttggc agaagcagta gaaacactcg ttttatcagg
     3121 acctgcaagt cagcaattga aacaactctt tgcgcttagc ccagtcattg atgctttctt
     3181 tgaaaatact atggtaatgg ctgaagatca ggctgtccgt caaaatcgtt tggcaatctt
     3241 gtcacaacta accaagaaag cagctaagtt tgcttgtttt aaccaaatta acactaaata
     3301 aaatttgata aacggacttt atcttattac aaaggagaag aaatggatcc gaaaaaaatt
     3361 gctcgtatca atgagcttgc taaaaagaaa aaaacagaag gcttaacacc agaagaaaaa
     3421 gtggaacaag ccaaactacg tgaggagtac atcgaaggtt atcgccgcgc tgttcgtcac
     3481 cacattgaag gaatcaaaat tgtggacgaa gaaggaaacg atgttacacc agaaaaacta
     3541 cgccaagtac aacgtgaaaa aggattacat ggccgtagtc ttgatgaccc aaattcataa
     3601 tatgtactct ttcttttaac aaagatagat gaaacgataa caaagagact agcagtttgt
     3661 gtttgctagt cttttttcgc taaaaaagga accataatgg ttcctaaaaa ctat
//
LOCUS       DAVBHS010000164         3668 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVBHS010000164 DAVBHS010000000
VERSION     DAVBHS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.164"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            233..647
                     /locus_tag="VQ651_001872"
                     /pseudo
     CDS             233..647
                     /locus_tag="VQ651_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(686..1129)
                     /locus_tag="VQ651_001873"
     CDS             complement(686..1129)
                     /locus_tag="VQ651_001873"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HET2754824.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(1159..3381)
                     /locus_tag="VQ651_001874"
     CDS             complement(1159..3381)
                     /locus_tag="VQ651_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET2754825.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
BASE COUNT          993 a          869 c          685 g         1121 t
ORIGIN      
        1 atttatcatt ttatgacaaa catactatcg ttaaatgctg atttaatcga aattttgagt
       61 tataaactcc tcagtttcga actcaaaatt agccgtgcag gataggatac ccttcataaa
      121 cttatataac tactttaata ccttaaaaag ctaataaaat aaggaatatc taagcaatat
      181 acattctaaa atacctgtac gggagtgtct ctaaagaaaa ggaggtttta gcatgaaaaa
      241 aatcatcttc atcaaaacca ttcaactcct tgtcattgat ggaatcatgc tggcattttt
      301 gacatttaaa aggggggctt acttgggact ggattttgat ttatagtggt tggctcattt
      361 tctttcatcc tgtgctattg acctatcttt caaaccaact ttgtgaccac tttagtcaac
      421 tctattccca gattagaccg agattctggc gttttgcttt acaaattctc ctatgggata
      481 gcctgatgat tctctccttg gtgtctttaa gtgatattcc acttttcctt cagggaactc
      541 tcctcatcct aggacatctc atcccttcct atcgcatctg ccaaagcctg aaaagagact
      601 tcccccaagc atatcaagaa ccgatttctt tttggagtat tttatgatcg atgagaaaga
      661 ccaagccgac tgggcttggt ctttcttatc tctttttagt atctaggata atggtaacag
      721 gtccgttatt aaccagctca acctgcatat ctgctccaaa gatacctgtc tgaacgggca
      781 cttcttgcgc taatttttga ttgaaagcat catagaagtc tgatgccata tcaggtttag
      841 ctgcccctgt aaaggctgga cgattgcctt tcttagtatc cgcaaagagg gtaaactgag
      901 aaatagagag gatttctcct tcaatatctt tgacagacag gttcatcttg ccttctgcgt
      961 ctgaaaaaat ccgcatattg accagttttc tcacagcata gtccaaatct tcctcttggt
     1021 cctctggtcc aacaccaacc agcaataaaa gtccctgatt gatttttccc tgaatctggc
     1081 cttctatact cacttgggct tttttaaccc gttggataat gattttcata atagcctttc
     1141 tagtaagagc taggacaact agccgttggt ccgtttgaca gagtaaactt ctggcacact
     1201 cttaatttta tcgacaaccg tggtcagtgt agagaggttg gcaataccga aggacacatg
     1261 gatattagca aacttcatat ccttggttgg ttgggcattg accgttgaaa tattcttggt
     1321 tgtatttgaa agaacttgca gtacatcgtt caacagtcct gtacggttga gaccgtagat
     1381 atcgatatgg gccatatact ccttatttga gctagagtac tggtcttccc attccacatc
     1441 aaggagacgt tgctcgtagt tttcttgggc acgcaggttc atacagtcca cacggtgaat
     1501 agccacacca cgacccttgg taatgtagcc aacaatatcg tcaccaggca cggggttaca
     1561 acacttagca atccgcacta ggagaccaga agcaccttca ataaccactc ccccctcatg
     1621 cttgaccttg agagtttctt tattttcaac cttgacctcg ccacctttga caagctcctc
     1681 tgcctcagcc ttggccttgg cacgctcttc ctcacggcgt tccttttcag tcagacggtt
     1741 aaagacggta atcgcaccga tttccccaaa accaatggcc gcaaagaggg agtcttctgt
     1801 cttgtaactg gtcttttgca gaacttgatc catgtggcgc ttgtccataa atttatttgc
     1861 cacatagcca ttttcttgga actgagccat cagcatctca cgacccttgt tgacagacaa
     1921 ttccttatct tggtttttaa agaactggcg aatcttattg cgcgccttgc tagtcttgac
     1981 catattgagc cagtcacggc taggtccaaa ggagttcggg ttggcgataa tttcaacctg
     2041 atcccctgtc tttaacttgg ttgtcagtgg aaccatgcgg ccattgacct tggcaccagt
     2101 tgctttttca ccgaccttgg tatggatttc gtaggcaaaa tcaatcggtc ctgaatcttt
     2161 gggaagagaa cggacagctc catctggggt aaaaacgtaa atctcctcag ccagatagtt
     2221 ttccttaaca gagtccacaa attccttagc atcatcagcc tggtcttgga gctccatcat
     2281 ctccttgatc cagttcattc caatagctga ttccttgctg ttaacttgcc cctttatacc
     2341 tttcttataa gcccagtgag ccgcaacccc gtactcagcc acctcgtgca tttccttggt
     2401 tcgaatctgg aattcaatcg gcccttttgg tccataaaca gtcgtatgga tagactgata
     2461 accattagcc ttgcggttgg caatatagtc tttgaagcga cctggcatcg gtttccaaaa
     2521 ttcatgcacg taaccaagca tggcataaac atcactttgg gtatctaaaa tacaacgaat
     2581 agcaatcaga tcatagattt cctcaaaccg ttttctcttg tcctgcattt tgcggaaaat
     2641 tgagtaaata tgcttgggac gaccataaat cttccctttc aagtgacgtt ctgtcgtata
     2701 ctcctctaat tttgtgacta cctcatccac caaggcctca cgctccctgc gcttttcctt
     2761 catcatatgg gtaatcttgt aaaactccgt tggattgaga taacggaaag acaagtcttc
     2821 taattcccat ttgacactgg aaatccccaa acgatgggca agcggggcat agatttccat
     2881 ggtttctttg gaaatacgct cctgcttgtc ttttcgaaga tgtttcaggg tccgcatatt
     2941 gtgcaagcgg tcagacagtt tgaccaaaat aacgcggatg tcctcagaca tggccatgag
     3001 catcttgcga tgattttccg ctaattgctc ctcgatcgat ttgtactcga ccttgccaag
     3061 cttggtaact ccgtcaacaa tcatccgcac atcaggacca aactctcttt ccaaatcgtc
     3121 caaagtcgca tctgtatctt ccaccacatc atgcaagaat ccacaagcta ctgttacagc
     3181 atccagcttt agcttagcta aaatacctgc cacttggata gggtgaatga tataaggctc
     3241 gcctgatttg cgatattgac cactgtggca ttcaacagca tagaccaagg ccttatggac
     3301 aaaatgaaca tcctcttccg ttaaatattc tttggttaaa gcgacaactt cttcgcctgt
     3361 taaattcact tctttcggca tctctactct ccaattcttc ctaccatttt atcacttttt
     3421 taagaatatg aaaactagat tggaacagaa taagaaaaaa ataattcaaa attgcttgat
     3481 aattctgaat tattggccca taatatacta cgaagttaga ttttaaactt aggtgataga
     3541 aggagagata gaagaacgga aaccatattg taacccaaag actttctgat ttccccaatt
     3601 ccattgaaga tacgaaagat aaacggtgga actcgtatca catacactgg taccttgact
     3661 ggattttg
//
LOCUS       DAVBHS010000165         3641 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVBHS010000165 DAVBHS010000000
VERSION     DAVBHS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.165"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..1349)
                     /locus_tag="VQ651_001875"
     CDS             complement(<1..1349)
                     /locus_tag="VQ651_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HET2754826.1"
                     /translation="MFKVMRGGVDTTQVMTETVEDKVSHSITGLDILKGIVAAGTVIS
                     GTVATQTKVFTNESAVLEKTVEKTDALATNDTVVLGTISTSNSASSTSLSASESASTS
                     ASESASTSASTSASTSASESASTSASTSISASSTVVGSQTAAATEATAKKVEEDRKKP
                     ASDYAASVTNVNLQSYAKRRKRSVDSIEQLLASIKAAVFSGNTIVNGAPAINASLNIA
                     KSETKVYTGTGLDSVYHVPIYYKLTVTNDGSKLTFTYTVTYVDPTTRALGNLSKMGHG
                     YSIYNTGTSIQTMLTLGSDLGTPSDVKNYITDKDGVLVRSYNTSTMTTWGSGYTWGNG
                     AQMNGFFAKQGYGLTSSWTVPITGTDTSFTFTPYAAKTDNIRTNYFKGRGKVVESSTT
                     SQSLSQSKSLSVSASQSASASASTSASESASTSASASASTSASASASTSASASASTSA
                     "
     gene            complement(1347..1460)
                     /locus_tag="VQ651_001876"
                     /pseudo
     CDS             complement(1347..1460)
                     /locus_tag="VQ651_001876"
                     /inference="COORDINATES: protein motif:HMM:TIGR03715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
     gene            complement(1698..2159)
                     /locus_tag="VQ651_001877"
     CDS             complement(1698..2159)
                     /locus_tag="VQ651_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2754827.1"
                     /translation="MGKERRKQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(2126..2269)
                     /locus_tag="VQ651_001878"
     CDS             complement(2126..2269)
                     /locus_tag="VQ651_001878"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2754828.1"
                     /translation="MAYSTDFKQGALDSIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KGT"
     gene            2538..2918
                     /locus_tag="VQ651_001879"
     CDS             2538..2918
                     /locus_tag="VQ651_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2754829.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            3017..3238
                     /locus_tag="VQ651_001880"
     CDS             3017..3238
                     /locus_tag="VQ651_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET2754830.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            3255..3569
                     /gene="trxA"
                     /locus_tag="VQ651_001881"
     CDS             3255..3569
                     /gene="trxA"
                     /locus_tag="VQ651_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET2754831.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
BASE COUNT         1098 a          738 c          697 g         1108 t
ORIGIN      
        1 gcactcgttg atgcggaagc tgacgcactc gttgatgctg aggctgaggc acttgtactt
       61 gctgaggctg atgcgctggt tgaggccgat tcagaagctg aggtgcttgc tgaggctgag
      121 gcgctttgac tagcacttac tgagagtgac ttagactgtg aaagtgactg actggtcgtg
      181 ctagattcaa ctacctttcc tctacccttg aagtagttag ttctaatgtt atctgtttta
      241 gcagcgtaag gggtaaatgt aaaggatgta tccgttccag taattggtac agtccaagat
      301 gatgttaatc catatccttg cttagcaaag aaaccattca tttgggcacc atttccccaa
      361 gtatacccac taccccacgt cgtcattgta gatgtattat aggatcgaac cagtacacca
      421 tctttgtcag taatgtagtt ctttacatct gaaggggtac caagatcact gccaagggtt
      481 aacattgttt gtattgaagt acctgtatta tagatagaat acccatgacc catctttgaa
      541 agattaccaa gagcccttgt ggtaggatct acatacgtaa ccgtataggt aaaggtcagt
      601 tttgaaccat catttgtcac tgtcaattta tagtaaattg gaacatgata taccgaatct
      661 aaacctgtac ctgtataaac ttttgtctca cttttagcaa tgtttagact tgcattaatt
      721 gcaggggcgc catttacaat cgtattgcca gaaaaaacag cagcttttat agaagccagc
      781 aattgctcga tggaatccac tgaacgcttg cgtcgcttag cataagattg gagattgaca
      841 tttgtaactg atgctgcata atcactagct ggtttcttac gatcttcttc gaccttctta
      901 gcagttgctt ctgtagcggc agctgtttgt gatcctacca cagtagatga tgcagagata
      961 cttgtcgaag ccgatgtact tgctgattct gatgcacttg tactcgctga ggtcgaagcg
     1021 ctggttgagg ctgactcaga tgcagatgta cttgccgact ctgaagctga caaactagtt
     1081 gaactcgctg aattacttgt agatatcgta cctagaacta ctgtatcatt tgttgccaaa
     1141 gcatccgttt tctctacagt tttttcaagt actgccgact catttgtaaa taccttcgtt
     1201 tgagttgcaa cggttccact tatgacagtt cccgcagcaa ctattccctt gaggatatca
     1261 agcccagtaa ttgaatgact tactttatct tctaccgttt cggtcatgac ctgagtagta
     1321 tcaacaccac cacgcatgac cttaaacaag ccaaaaagag aagtcgaggc ccgtagccaa
     1381 tgcttccctg atttgattaa tttgaaccga gttactcggt ctgtttctct atattcaccc
     1441 ttctgacgtc taaaaaatat tttgaaattt cctttcgagt attattttca tgtatggata
     1501 taaataaatt accatactat aattatagaa tagggaaccc aaaatagcaa gaaacttgag
     1561 gtatttttgc taaaaaaaaa aagcatttta ctattttata tatatattat aagataagtt
     1621 ttttataaag acacgcaaat gagtataaat cagctaattt ttggtttata ttcctatttt
     1681 tatgcataat tattctttca ttctctaatt tgtgaatcaa tatatctgat tagaaaacta
     1741 ccattgagtt tttgctccac ctcattagtc cgctcaaaac gccgtccgct aatcttgtca
     1801 tagactttct cccctctagg agcccctgca taaggacgat agaggtagcg gttgattccc
     1861 gtttcgtcaa tatataggac tggtaaatct tttaggttat ccaaaatatc aagaaactca
     1921 gaaactttct caggcttttg ttccctaaaa ctggtcgtct tttttaaaat gaccttaatc
     1981 tgctttaaaa ctgcccatac ggagggcaaa gcacaatcaa aacgggccgc aatttcccgt
     2041 aaaaaagcgt ctggatgagc ctctacaaag gctttcaatt cttctaaagg gatcttacgc
     2101 tttttgacga ctcgcttttt ccgctctaag ttcccttgtt tacgtctttc ttttcccacg
     2161 tgaagagagt tctgacgcca acaccaaaaa acttggctgc ctcgacatga ctgtgcccct
     2221 ctttgatgga atctaatgct ccttgtttaa aatcagtact atatgccata gctttattag
     2281 accatgctta ttttaaacta agcaactata gatagagaaa tgaaataaaa tctaaattcg
     2341 aatgtgataa ggagttagaa attgaaacaa taggattccc ccattattca cacaaaaata
     2401 aaaccactaa aaaacagagc ttgccagcat tcttgaaaag tagtataatt attgcatgcg
     2461 caatcatctt gtgacgcaga gactgcccgt taatggactt tcttctatat tatgactcta
     2521 aaaagaaagg agacactatg acctatttgg aaaaatggtt tgacttcaat cggcgtcaga
     2581 aagaaattga aagtctcttg gaagagacca ttgcccaaca gagcgagcaa agtctgacct
     2641 tgaaagagtt ctacctgctc tactatctgg acttggctga agaaaaatct ctacgccaga
     2701 ttgacctgcc agataaactt catctgagcc cgagcgcagt ttctaggatg gtggctcgct
     2761 tggaagctaa aaattgcggt ctactcagtc gcatgtgttg ccatcaagat agacgctcta
     2821 gttttatctg cctgacaaat gatgggcaaa agacactggc ctcgctacaa aagactgtcg
     2881 aagaaagctt ggaagcaggt ttggatttct tgatttaaga tgattttaaa aaaagttgcg
     2941 tgcgcaatca tttttcttga cattctcttt tataaggagt aaaataaagt tagcattaaa
     3001 caaaggagtt ttaaatatga ttgaaattac ctatatagat gccagcaaga acgaaagaac
     3061 tgtgactttc gagtcttatg aagactttga acgttcacaa caagcttgcc ttatcggcgt
     3121 cgcagactac taccctgtcc aaaaattaac ttacaagggt cataatttgg actaccatgg
     3181 gacttatgga gatatcttct tctatctcat gaaacaagat ttaagccaat ataactaaaa
     3241 aaaggagaaa tacaatggca aaagcaatta cagatgcaac attcgaacaa gaaacaaaag
     3301 acggtttggt cttagtagac ttctgggcaa cttggtgtgg tccatgtcgt atgcaaggtc
     3361 caatcttgga caaattgtct gaagaacttt cagaagatgt cttgaaaatc gttaaaatgg
     3421 acgttgatga aaatccaaac acagctcgtg cttttggaat catgtctatt ccaactcttc
     3481 tcttcaaaaa agacggccaa gttgtcaaac aagttgcagg tgttcacaca gcagaacaaa
     3541 tcaaggccat cattgctgaa ttgagctaat caaacgagag accaagtact ttctttggtc
     3601 tcttttttct tgccctttgc catttttcaa aaaatatgct a
//
LOCUS       DAVBHS010000166         3637 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVBHS010000166 DAVBHS010000000
VERSION     DAVBHS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.166"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(119..970)
                     /gene="rfbD"
                     /locus_tag="VQ651_001882"
                     /pseudo
     CDS             complement(119..970)
                     /gene="rfbD"
                     /locus_tag="VQ651_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
     gene            complement(1036..2085)
                     /gene="rfbB"
                     /locus_tag="VQ651_001883"
     CDS             complement(1036..2085)
                     /gene="rfbB"
                     /locus_tag="VQ651_001883"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET2754832.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLVAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGIYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQATD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            complement(2095..2691)
                     /locus_tag="VQ651_001884"
     CDS             complement(2095..2691)
                     /locus_tag="VQ651_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HET2754833.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     VLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENLEEADVSEADKHHPLLKDVKPLKKEDLE"
     gene            complement(2692..3549)
                     /gene="rfbA"
                     /locus_tag="VQ651_001885"
     CDS             complement(2692..3549)
                     /gene="rfbA"
                     /locus_tag="VQ651_001885"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET2754834.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGIKLSYAEQPSTDGLAIGEEFISDDSVALIL
                     GDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEKPEYP
                     RSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRGFAWL
                     NTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKNEYGQ
                     YLLRLIGEA"
BASE COUNT         1012 a          850 c          651 g         1124 t
ORIGIN      
        1 ctgagccaaa gctatgcacc aaagagacca gcaccaacaa caagataatt gtacatctta
       61 agctcctttg gagttcatta tctgcctctt ttattagact agaaaatcat tctactactt
      121 atctcacttc ttgtttgtaa aattcttgca atgcatcttg ccaagttgga ataacaaatc
      181 cagtagcttt ggctttggcc aggctcatcg ttgagtttag cggacgttga gctttggctg
      241 gaaattgact ggaatctact ggcttgactt cgacatctgt atctttcaaa atttcaactg
      301 caaaatcata ccatgttgtg tcttctgtcg catcatttga caaatgataa taaccaaatt
      361 ccttacgatt ttcagctagg tagatcatga attcagccaa ggtacgagtc caagtcggac
      421 gaccgtactg gtcatttaca actgttaaag tcttatgagt tttcgcaaga ttttgcatgg
      481 taaaaacgaa gtttttgcca taatttccaa atacccaggc agtacggata atatagaaat
      541 tagacacatg cttctcaact aactcttccc ccatacgctt agtgcgtcca tattctgtct
      601 gtggatctgg tcggtcatca acttcccact cttgtccaac tggtttctta ccgtcaaaga
      661 cctagtccgt agaaatataa actagagttg caccatgctt ttcagatgct tttgcgacat
      721 tttttgtccc catcacattg atggcgaagt ccaactcttt tccttcatcc tctgctgcat
      781 caacagcggt gtaggctgca caatggtaga ctaaagtcgg tttcacctct tcaaaaactt
      841 tctcaaccat ttcttcattg gtaatgtcca tctcagccac atctactgcc acgtattctt
      901 cattacgttc atccaataaa tagcgaagtt ccgttcctaa ttggccattt gcccctgtaa
      961 ttaaaatcat ctctagacct ttctaatatg taactcttcc caatatagca ataaaaagca
     1021 gcgatttcct gctttttata ctgtaataat ctcctgagtc ttagcataat tggcttcaac
     1081 agcttctttt tctgctttcc accattcttg gttatctgta taccacttga ttgttgcctt
     1141 gagcccagct tcaaagttgg taaattcagg tttccacccc aactcatcac ggagcttgct
     1201 ggcatcaatc gcatagcgaa ggtcatgtcc tgcacggtca gtcacatgat cataggcatc
     1261 cgtagcttgt cccatttcct taaggataag ttccaaaact tccttattgt tcttctcacc
     1321 atcagcccca atcaagtagg tttcaccgat ttgccctttt gtcaagattg tccaaactcc
     1381 tgaagaatgg tcattggtat gaatccagtc acgaacgttc ttaccttcac cgtaaagttt
     1441 tggcttaatt ccacttagga tgttagtaat ctgacgtggg atgaattttt cgatatgttg
     1501 ataaggaccg tagttatttg aacagttgga aatcgttgcc ttgactccaa aagaacgcac
     1561 ccaggctttg acaatcaagt ctgatgcggc cttggttgat gagtacggag agcttgggtt
     1621 gtattttgtt tcagcagtaa atttctcacc tggtccttca ccatgacctg gcaaatcttc
     1681 tcgtaaaggg agatccccat aaacttcatc tgtcgataca tggtggaagc gaatatcata
     1741 cttacgagca gcttctaaaa gagtatagat tccaatgaag ttagtatgaa taaatggcga
     1801 tggatcattg agcgaattat cattgtggct ttccgctgca taatgaacga tagcatctgc
     1861 ttgagcaacc aacttgtcta ccaactccgc atcagcaatg tcaccaacaa ctaactcaac
     1921 acgattgcct aaaatttcct caatattcgc gcggtttcca gcataagtca acttatctag
     1981 gactgtcacg tgaacatctg gaaagttctc gtaaacataa tggacaaagt tagaaccgat
     2041 aaagccagct ccacctgtca cgataatatt tttgtattca gtcatattct ttccttattc
     2101 caaatcttct tttttcaaag gcttcacgtc cttaagtagg ggatgatgtt tgtctgcttc
     2161 agatacgtct gcttcttcca gattttccca ttcaatacca aggcttggat cagcgtagtt
     2221 cacaaaggca tacttgggtt tgagttcaag agcccagtaa tcattgacca gatagctata
     2281 tgacactgta tctgataaaa cttggaagcc attagctacg cctcgaggaa caaagattcc
     2341 cttgcttgca tcaattactg tctgataggt attcccaaag gtttcaccct cgcgtagatc
     2401 aacccaagaa cccagaactt tccctccatc tgctacagag atgtacttat cccaaggctc
     2461 tgcgtggagg cctcgaagga catttttacg tgagaaggat acattgtttt gcaattttcc
     2521 ttctgcaaag aaagactctg gaaatccaag tggaagcact ttttcctttt ggaaattttc
     2581 tttaaaccag ccacgattat ctccatgaac ggggatatca aactccaaca tgcctggaat
     2641 agcttcaacc ttgcgtgccg caagcgtctt accgaaaaaa ttatctgtca tctatgcttc
     2701 tccaatcaaa cggagcagat actgtccgta ttcatttttc ttaagtggtt gggctaaggc
     2761 caatacatct tctcgactga tatagcccat acggtaagca atttcttcta agtttgctac
     2821 ctgaacattt tgcatccgtt gcactgtttc gatgtactgt gaagcctcta gtaaactttc
     2881 atgagttcca gtattcagcc aagcaaagcc acgtcccata agctcaacag acaaatcacc
     2941 acgatctagg taagccttgt ttacatctgt aatttccagt tcaccacgag ggcttggttt
     3001 aatactcttg gcaatttcga caacatcgtt atcatagaaa tacagtcctg tcactgcata
     3061 gttggagcga ggatactccg gtttttcttc tatggaaata gcattcatgt ctgtatcaaa
     3121 ctcaaccaca ccaaaacgct ctggatcctt cacttggtag ccaaaaacag tcgcaccttt
     3181 ctctttcttg gctgcttttt gaagcattgt gctcagacca ggaccatggt agatattgtc
     3241 gcctaaaatc aaggcaacgc tatcgtcaga aataaactct tccccaattg ccaaaccatc
     3301 tgtacttggt tgctctgcat aagaaagttt gatcccaaac tcagatccgt cttgaagaag
     3361 ctcttgaaat cgatgtaaat cctgtggagt ggaaataatc aaaatatccc taatcccagc
     3421 caacatcaat gttgaaagtg ggtagtaaat catcggttta tcataaaccg gcataagttg
     3481 ttttgacgca gcgcgagtca aaggatataa acgtgtcccc gaaccacctg ctagaataat
     3541 acctttcata gtagggtacc tttctttgtt attttagagt caattactat taattgtgta
     3601 ctgctctaaa aagaaaacaa tcatcccatg ttttata
//
LOCUS       DAVBHS010000167         3563 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVBHS010000167 DAVBHS010000000
VERSION     DAVBHS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.167"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(49..264)
                     /locus_tag="VQ651_001886"
     CDS             complement(49..264)
                     /locus_tag="VQ651_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HET2754835.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            362..682
                     /locus_tag="VQ651_001887"
     CDS             362..682
                     /locus_tag="VQ651_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754836.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(713..1051)
                     /locus_tag="VQ651_001888"
     CDS             complement(713..1051)
                     /locus_tag="VQ651_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754837.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(1063..1782)
                     /gene="trmD"
                     /locus_tag="VQ651_001889"
     CDS             complement(1063..1782)
                     /gene="trmD"
                     /locus_tag="VQ651_001889"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET2754838.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(1772..2290)
                     /gene="rimM"
                     /locus_tag="VQ651_001890"
     CDS             complement(1772..2290)
                     /gene="rimM"
                     /locus_tag="VQ651_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET2754839.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKRYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(2321..3109)
                     /locus_tag="VQ651_001891"
     CDS             complement(2321..3109)
                     /locus_tag="VQ651_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2754840.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
BASE COUNT         1035 a          777 c          592 g         1159 t
ORIGIN      
        1 atacggcaag gcaaagctga cgcggtttga agagattttc gaagagtatt agttgccttt
       61 aaaggcatcc accatcgttt gaaattcttc atttgagaga gtaatccctt tgcccatttt
      121 agtatggtct ggactccaag cacgaatatc aaactttgca ggggcaccat taaagctcac
      181 acggttaatt tccttggtcc aacctttttc gttttcagaa agagtcaaca agtgctcttc
      241 gatttcaaat gtaaattctg ccattttctt ctcctttttt agtttcatta gtttattcgt
      301 aaaatcttgt agattttagg aaaattttat ataatattga tataaaagaa gggaggccaa
      361 tatgagacat aaattccagc aagttctaaa taaaatacat gattttttaa atggatatga
      421 ccaacctgac cagactgaaa ccaactccct tacagccact attgaagagg ctatccagaa
      481 acaaaccgct gttcacctta tcttgtctga gacaagtttt acaggtgata tcatcaaata
      541 tgatcagcaa ggccagcaaa ttatcgtgaa aaattttgcc aaaaatgtga gccggattat
      601 ccgtataagc gatattcaac gcctgcgatt tgtcccctca actgtccaaa cagcccaaaa
      661 aaatagattt aagaaagagt gagatgtagt tgcttcatcc cactcttttt tcttagcgaa
      721 tttgttcaaa atgtaaatga actgcaatat gatctccata accacttctt tccaagtcac
      781 gttgtaaacg ataggaaatg tagtgttctg caatggtaat gtaacctgcg cccaataaac
      841 gatgttcaac catagattga atcatactga tagtcgcacg ttccaccttg gcttcttgta
      901 aatccaaaac taccttctta gtgacttgag caagattttg acgcaaatca tctgtcaaaa
      961 cataaacagt ttgggctgcc ttcaagatgg cttggtaaat cttatctgga ttaaattcag
     1021 caatttcgcc attacgtttg attacttgca taggtttctc ctttattctt tgttttcttt
     1081 gatttctgcc agcatttttt cttcttctac tgtcagttga taatgttcaa gtaaatccgg
     1141 tctgcgctcg taggttttct ttaaactctc gtacaatcgc cactgacgaa tcttttcatg
     1201 gtggccactc atcaatacat ctggcacgac catgcctcga taatcatagg gacgtgtgta
     1261 ctgaggatat tctaaaagac ctgaagaaaa actatcatct tggtggctag actccttgcc
     1321 aatcacttct ggaatcaggc gaactgtagc atcaatcatg gtcatagctg ccaattctcc
     1381 accagtgagg acatagtcgc ctagggaaat ctcatctgtt accaaggtct taatgcgctc
     1441 atcataaccc tcatagtgcc cacagataaa gattagctct tcctcttgag ccaaatcttc
     1501 agcataagcc tgatcaaact gctttccagc aggatcgagg agaataacgc gcggattttt
     1561 cttttcaata gcatcaaagg aatcgaaaat aggttgtgct ctgagcaaca tgccctgacc
     1621 gcctccgtag ggctcatcat ctacatgacg ggccttttca gcattttctc gaaaattatg
     1681 atactggata tccaagagcc ctttttctcg agcctttcca acgattgagt gctccagtgg
     1741 agaaaacatc tctggaaaga gggttaaaat atcaatcttc atcgtctaac ccttctaaga
     1801 tttccacatc gacccgttta tttggaatat caacattgag aaccactggt gggatataag
     1861 gtaaaagcaa atcacgtttg ccttttcgtt tgaccaccca gacatcatta gcacctggtt
     1921 gcaggatttc cttgatggtt ccaaccaagc tatcaccctc atagacttcc aaaccgataa
     1981 tctcgtgata gtaaaattca ccatcgtcta ggtcattcaa atcttcctca gcgaccttga
     2041 gactatatct cttgtacttt tcgatagtat tgatatggta catatctttg aatttaataa
     2101 tgtcaaagtt cttctgttta cggtggctag cgatggtcac tgtttggaca aactgatctt
     2161 tttcatcaaa caaagccagc tcagctcctt ttttaaaccg ttcttctgca aaatccgtca
     2221 cagacaagac tcgcatctca ccctgtaatc cctgcgtatt aacgattttc ccaacattaa
     2281 agtagttcat cttgtctcct gtaatctcct tttttccatc ttattctgac aattctcgaa
     2341 taatagccgc aattttttcc gattctgacc attgtaaata atggtgattc cctcctaaaa
     2401 tgagtttagt attggaagtc caatattctg attctctgta ctctttttct ctataaggct
     2461 gacaaaaaac aaatacagga atatgagctt ctatagatac atcctcaaaa tcttcctcag
     2521 taatctctcc agatatctga aattctggat cttgattttc caactctaag cctttttctt
     2581 gcattaattc ccagattttt ttattcgttt caggactaaa tgttgcttga gttaagttct
     2641 taaaataaag ttcaggacca cactcgtcaa tcagcctcat ctgctcttcc atttctggat
     2701 aaggattttc tgaaaaatca gcaaacatga cttttttagt tgtcggttca attgctacta
     2761 aagtctgacg cttaattggt ttctcgagta atttgcaagc taaaattcca ctccaactat
     2821 gtgcacaaag tatatattca gaaattccta attcttcaag tacttcataa accgcatctg
     2881 caagattatc tagatttttt ccagcttggt catgaatcgg actcctacct gtgttcggaa
     2941 aatcaattgt caaataacca attgtaggag gaggtttttc aagtataagt gaaaaatttt
     3001 cataacttgg tagcaaacct gctccgttta gacaaactag cactttcttt tgcttttgat
     3061 aagtaacaga gaggctacca atttctgtag atacttcaaa cctcttcata aagaaatcca
     3121 ctcattctat ataatgaatt attaaaaatc cttatctttt attttatcac gttccaagga
     3181 ttttctcaag ttggaggaag gggacaacat ctctactttc ccttcaaaaa tccttccaaa
     3241 tcatgtttat gttggtaatt aacggctgcg gttttgtctt tctcaaagac agtcttggta
     3301 aggtcaatat gattaatagc tacgattgcg acggtgtagt aaatgatatc agccagttct
     3361 ctggcaagtt cctcgttcga atcctatccc ttcttttcga ccagagcgcc tattcaaaac
     3421 ctcggctact tctccgactt cctccactaa cttcataaag agaccttcat cagtccgaga
     3481 ctgctgttaa tgttcgatta agtagtcttg gaattgccta aacgttcaat cttttatagt
     3541 atattgaaac tagaatagta cac
//
LOCUS       DAVBHS010000168         3548 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVBHS010000168 DAVBHS010000000
VERSION     DAVBHS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.168"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(13..243)
                     /locus_tag="VQ651_001892"
     CDS             complement(13..243)
                     /locus_tag="VQ651_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET2754841.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            complement(253..1494)
                     /locus_tag="VQ651_001893"
     CDS             complement(253..1494)
                     /locus_tag="VQ651_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET2754842.1"
                     /translation="MVLPNFKENLGKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFKNAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            complement(1516..1668)
                     /locus_tag="VQ651_001894"
     CDS             complement(1516..1668)
                     /locus_tag="VQ651_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754843.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            complement(1689..1967)
                     /locus_tag="VQ651_001895"
     CDS             complement(1689..1967)
                     /locus_tag="VQ651_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HET2754844.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            complement(1960..2223)
                     /locus_tag="VQ651_001896"
     CDS             complement(1960..2223)
                     /locus_tag="VQ651_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HET2754845.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            complement(2325..>3548)
                     /gene="polC"
                     /locus_tag="VQ651_001897"
     CDS             complement(2325..>3548)
                     /gene="polC"
                     /locus_tag="VQ651_001897"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="catalyzes DNA-template-directed extension of the
                     3'- end of a DNA strand by one nucleotide at a time;
                     required for leading strand synthesis; PolC exhibits 3' to
                     5' exonuclease activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HET2754846.1"
                     /translation="HPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQTTHFNFHDIDEN
                     VLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVLGVTPEQIGTPTG
                     MLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQDLIKQGIADLSTV
                     IGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIEAMKANKVPEWYI
                     ESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAKAFDIKTMGAGLE
                     VIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLDLYRSQATEFLID
                     GDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSSTLVEKMDEMGILG
                     NMPEDNQLSLFDELF"
BASE COUNT         1004 a          887 c          654 g         1003 t
ORIGIN      
        1 agatttagga aattaacttc ctcgtctcca aaaaatagct aaaacaatca tggctcctaa
       61 aatcgctggg ataatagctg ttcctgataa aactggcccc caagttccaa agagcaagtg
      121 acctagaaag gctccgatcc aaccgagaaa catttttcca aaacatccca ttcgctctcc
      181 acgattggtc atagcacctg ctaaaaatcc cactaggaga ccaacgaaca tacttcctaa
      241 catattatct ccttaatttg cccaattccc attacggaaa agaggtaccc gcgttccatc
      301 ctcacgaata ccatcgatat ccatttggtt agaaccaatc ataaagtcta cgtgaacatc
      361 tgaacggtta agccctgcag cttcaagctc ctcttcgctc atctccgctc catcaacaac
      421 gctagtcgca taggctgcac cgatagccaa gtggtttgac gcattttcat cgaaaagggt
      481 gttaaaaaag gtaatgcctg actgagaaat tggacttgga tctggtacca aggcacattc
      541 acccaaggca cgcgcacccg catttttaaa gacaaggtct ttcataacct gatcaccctt
      601 ctcagcagtg atatctacga tttgtccatc cttaaaggtc accttaatgc cttcaatgat
      661 atttccgttg tagctaagcg gttttgtaga agtgacataa ccatctgcac gacggaagtc
      721 aggcgctgtg aagacctctt ctgtcggcat atttggcaag aattcttcgc cctgtgcatt
      781 gacagcacca gctgattccc aaacgtggtt ctttggcaaa ccaagtgtta aatctgttcc
      841 tggcgctgtg tagtgaaggg ctgaaaattg ctccttatta agcatatcgg ccttgctctt
      901 gagaatggct gcatgttcct cccaagcctt aacaggatct gcttcgtaga cacgacaagt
      961 tttgaagatt tgatcccaaa ggaaatcaac tgcttcttcg tcgctcgcag catttgggaa
     1021 gactttctta gcccactcaa gtccagcagc ggctgctaca gtccagctaa ccttgttaga
     1081 ttgagttgcg atacgcatag gcttcatggc aagtcccata gctttagcag aagctgaaag
     1141 cttgtcagcg tccactccgt tcaaggcacc tggatcagat gaacgaaccc caagacggct
     1201 agccttgttc tccaaaagat agttcatctc agcaatcttg tattctggca cattgtccaa
     1261 acgctccatc ggggcatgga ggaatttctc acggttaatc acatcatctg tccactgaac
     1321 gatgacctca tgcgcaccca aggcataagc ttctttcacg attagatgtg ccaattcacg
     1381 ttgctccaca tcaatagaga gagccaaagt gtgaccaggt tgcacgttaa ttccgttcgc
     1441 aaccaacaat ttcgcatatt ttcctagatt ttctttaaaa tttggtaaaa ccattctgtt
     1501 ttctcttttc tatttttatt tttctttcaa agcagaaaag attcctgcta aagcaatagc
     1561 acctgacaag agtgctgtcg atagaagaat tgtacgatca gcaagttcta attgatattc
     1621 aaataccttc ttagccggct tatcttccgc aaatattctt agtttcatga gaaccccctt
     1681 aaaatagatt aaaataaatc actgtgactg cctgtcctca gcaaatttaa aatcaattcc
     1741 gacttgtcta ctttataaac caaaagccaa tctggctggg tatggcattc acgaactcct
     1801 tgaaaatgct tggatgccgt caatgaatga tcacgatatc tggcaggatg ttctttttct
     1861 tgaaccagaa aattcaaaac ttcttctaat aattctgcct tcaaaccacg cttcatagcc
     1921 aacttaaaat cttttttaaa ctgtttatga taacgaatct taagcacgta agtcctccat
     1981 caaatctgag actgattcaa aagactggct catattacga ttttggtcta aatccgttaa
     2041 cgcttggagc aacttccgat tttcgtctag tctaacatca aaaggtaatc cctgatattg
     2101 aattgcctga cgaaggaaaa tattaatagc tgttgtcata tccattccca gattactaaa
     2161 cacctgttgg gcctgctcct taacctcact atccagacgg atgcttatac tcatctttga
     2221 catactctca ccctctttcc atagactatt ttaacaaaaa agaaagctaa tgtaaatcta
     2281 ttggatatac gttagcctct tttaatagat tattaagcaa ttttttaaaa caactcatca
     2341 aacaaactca actggttatc ctctggcata tttccaagaa tacccatctc atccatcttt
     2401 tcaaccaagg ttgatgagag tccaccacgc ttgcgtagtt ctgttttaga gaggaattct
     2461 ccctcttcac gcgcccgcac cagttgcttg gcaacgttct ctcccagacc atccattgct
     2521 acaaatggtg ggataagggt atccccgtcg atgaggaact ctgtcgcctg actacggtag
     2581 agatctaatt taccaaactt gaaacctcgt tcccacatct cattgacaat ctcaagagtt
     2641 gtatagagat cgatttccac attagaggct tcattgttct tccgtttttc agagatttct
     2701 tccattctgc gcttgatgac ctccaagccc gcacccatgg tcttgatatc aaaagcctta
     2761 gcacgaatgg agaagtaagc acagtagtaa taaataggat ggtgaacctt gaagtaagct
     2821 acacgcaagg ccatcataac gtaggctgcc gcatgggcct tagggaacat gtacttaatt
     2881 ttcccacagg attcgatata ccactctggc accttattag ccttcatggc ttcgatatag
     2941 ccatttctct cctcttctga aatctttagc cacaaaccct tacgtacccg ttccataatg
     3001 gtaaaggcca tcttaggttc cagacccgca tgcatgaggt aaaccatgat gtcgtcccga
     3061 caaccgataa cagtcgatag gtccgctatt ccttgcttaa tcagatcctg agcattcccc
     3121 aaccaaacgt cagtaccgtg ggacagacca gacagctgaa gcaattccgc aaaggttgtc
     3181 ggatgggttt cgtctaccat tccacgtacg aaatttgttc caaactctgg aatccccaac
     3241 atacccgtag gcgttccaat ttgttcaggt gttaccccta gtacatcagt cccagaaaag
     3301 agtgccatca cgccttcgtc atccatagga attttattag ggtcaatacc agacaaatcc
     3361 tgaagttttc gaatcatggt cggatcatca tgtcccagta catcgagttt gaggacgttc
     3421 tcatcgatat cgtggaagtt aaagtgagtg gtctgccatt cagccgtgac atcatctgct
     3481 ggatactgga caggcgtaaa atcgtagaca tccatgtagt tcggaataac aacgattccc
     3541 cccgggtg
//
LOCUS       DAVBHS010000169         3507 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVBHS010000169 DAVBHS010000000
VERSION     DAVBHS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.169"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            22..882
                     /locus_tag="VQ651_001898"
     CDS             22..882
                     /locus_tag="VQ651_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET2754847.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            1093..1803
                     /gene="ccdA2"
                     /locus_tag="VQ651_001899"
     CDS             1093..1803
                     /gene="ccdA2"
                     /locus_tag="VQ651_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET2754848.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            1823..2389
                     /locus_tag="VQ651_001900"
     CDS             1823..2389
                     /locus_tag="VQ651_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HET2754849.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            2400..>3507
                     /gene="msrB"
                     /locus_tag="VQ651_001901"
     CDS             2400..>3507
                     /gene="msrB"
                     /locus_tag="VQ651_001901"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET2754850.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYV"
BASE COUNT          969 a          645 c          779 g         1114 t
ORIGIN      
        1 ttataaatga aggagtatga catgaaaaag tggtggaaag agctgataga caagccttta
       61 ttaaaagctt ttttgcatta ttatcaagca tcagatagtg agttgaccag tgtcgcagta
      121 gcctactatt ggttgatttc gattttcccc ctgcttttgg tggtggtcaa tatcctcccc
      181 tattttcaga ttcctgtggg agaatttctg tgctttatga aagacgttct gcccccaagc
      241 ctctatgaag gtgtggaaaa aatagccaga gaagttttga cgcaaccttc aacaggttta
      301 ttgagttttt ctgttttatc ggcgttatgg agtttttcaa aatctatgaa tttcttgcaa
      361 aaagctttta acaaggctta tggcgtcgaa aagagtcgtg gacttatttc ccatcagatg
      421 ctaagtctcc tagtcagttt tggcttgcag ctcctctttg cgtttgcctt gtttttgatc
      481 ttatttggtc agatgatttt ggctttactt gctcattatt ggacaaaaga tggcattatc
      541 tatcaggctt tacaagggtt ggcaggtcct ctgatttacg ccctcctctt tgctatcttg
      601 gtcatgcttt attattttct tcccaatcta tctaacagga aaattagcta taccctacca
      661 ggcagtgcct ttgttctctt agtgatttta ggtttactaa ctctgttctc tagttatctc
      721 aattactatg tccatcattt ggtggatgtc cgaattttag gttccgttct tcttgttgtt
      781 atgatgtttt ggtttatctt gattgctaaa attgttatcc taggcgcggt tatcaatgcc
      841 agtatgcaga gtttgaaaga tccggtcttt aaaaaagact aactatttat ccattacaaa
      901 agcgcatagt atcaaaactt tatagaacct tgatactatg cgcttttgtg ctttgacagt
      961 tttttgtata gtttttgtcc aagatttcta aatcatctga ttaaattcct attttcccct
     1021 atcattgtcc ctgttttttc gaaaatggaa ggagtataat aaatctatca ataaaatgat
     1081 aggaggaact ttatgggtca tattttcttt tttctaagtg tctttttggc agggattcta
     1141 tccttctttt ctccttgtat cttacctttg ttaccggtct atacaggagt gttactagat
     1201 gataaggatg gtgctcaggc ttctagcggc aaattttcaa tctcagttac tagtttatta
     1261 cgaactctgg cctttatagc aggaatttcc tttatattta ttttgttggg ctatggagct
     1321 ggttttttag gcgatttgct ttatgcttct tggttccaat atcttactgg ggcaattatt
     1381 atccttcttg gtttgcacca aatggagatt ctacacttta aggggcttta taaggaaaag
     1441 aggctacaac tgcaaggaca ggggcaaaat ggtaagggct atagtcaggc atttttattg
     1501 ggcttgacct ttagttttgc ttggacgcct tgcgtggggc cggttctggg gtctgttttg
     1561 gccttggcgg cttcaggtgg ttcaggagct tggcagggag ctggtctcat gttggtgtat
     1621 acgctgggct tggcgctacc attcttgctt ctagctctga cctctagtta tgttttgaaa
     1681 catttccgaa aacttcatcc ctatctcgga atcctcaaaa aagtgggtgg ttttctcatt
     1741 attgtgatgg gactcttggt tctgtttgga aatgcttcaa ttttaagtca attatttgaa
     1801 taaaatggaa aggaatatca atatgaaaaa atggcaaaca tgtgttcttg gagcaggttc
     1861 gctcctttgt ttgacggctt gttcaggcaa gtccgtgact agtgaacacc aaacgaaaga
     1921 tgaaatgaag acggagcaga cagctagtaa aacaagcgca gctaaaggga aagaggtggc
     1981 tgattttgaa ttgatgggag tagatggcaa gacctaccgt ttatctgatt acaagggcaa
     2041 gaaagtctat ctcaaattct gggcttcttg gtgttccatc tgtctggcta gtcttccaga
     2101 tacggatgag attgctaaag aagctggtga tgactatgtg gtcttgacag tagtgtcacc
     2161 aggacataag ggagagcaat ctgaagcgga ctttaagaat tggtataagg gattggatta
     2221 taaaaatctc ccagtcctag ttgacccatc aggcaaactt ttggaaactt atggtgtccg
     2281 ttcttaccca acccaagcct ttatagacaa agaaggcaag ctggtcaaaa cacatccagg
     2341 attcatggaa aaagatgcaa ttttgcaaac tttgaaggaa ttagcctagg aggtgtctta
     2401 tgaatgataa gttaaaaatc ttcttgttgc taggagtatt ttttctagcc ataaccggtt
     2461 tctatgttct attgatacga aatgcagggc agacagatgc ctcgcaaatt gaaaaggcgg
     2521 cagttagcca aggaggaaaa gcagtgaaaa aaacagaaat tagtaaagac gcagacttgc
     2581 acgaaattta tctagctgga ggttgtttct ggggagtgga ggaatatttc tcacgcgttc
     2641 ccggggtgac ggatgccgtt tcaggctatg caaatggtag aggagaaaca accaagtacg
     2701 aattgattaa ccaaacaggt catgcagaaa ccgtccatgt cacctatgat gccaagcaaa
     2761 tttctctcaa ggaaatcctg cttcactatt tccgcattat caatccaacc agcaaaaata
     2821 aacaaggaaa tgatgtgggg acccagtacc gtactggtgt ttattacaca gatgacaagg
     2881 atttggaagt gattaaccaa gtctttgatg aggtggctaa gaaatacgat caacctctag
     2941 cagttgaaaa ggaaaacttg aagaattttg tggtggctga ggattaccat caagactatc
     3001 tcaagaaaaa tccaaatggc tactgccata tcaatgttaa tcaggcggcc tatcctgtca
     3061 ttgatgccag caaatatcca aaaccaagtg atgaggaatt gaaaaagacc ctgtcacctg
     3121 aggagtatgc agttacccag gaaaatcaaa cagaacgagc tttctcaaac cgttactggg
     3181 ataaatttga atccggtatc tatgtggata tagcaactgg ggaacctctc ttttcatcaa
     3241 aagacaaatt tgagtctggt tgtggctggc ctagttttac ccaacccatc agtccagatg
     3301 ttgtcaccta caaggaagat aagtcctaca atatgacgcg tatggaagtg cggagccgag
     3361 taggagattc tcaccttggg catgtcttta cggatggtcc acaggacaag ggcggcttac
     3421 gttactgtat caatagcctc tctatccgct ttattcccaa agaccaaatg gaagaaaaag
     3481 gctacgctta tttactagat tatgttg
//
LOCUS       DAVBHS010000170         3478 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVBHS010000170 DAVBHS010000000
VERSION     DAVBHS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.170"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            331..1377
                     /gene="pheS"
                     /locus_tag="VQ651_001902"
     CDS             331..1377
                     /gene="pheS"
                     /locus_tag="VQ651_001902"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET2754851.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1377..1886
                     /locus_tag="VQ651_001903"
     CDS             1377..1886
                     /locus_tag="VQ651_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2754852.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1963..>3478
                     /locus_tag="VQ651_001904"
     CDS             1963..>3478
                     /locus_tag="VQ651_001904"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET2754853.1"
                     /translation="MLVSYKWLKELVGIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEILSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKKFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYDKAVNFKEFTLTETNEAATDALSVGIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SHVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRT"
BASE COUNT          995 a          669 c          857 g          957 t
ORIGIN      
        1 tcatcttatt tcaatccgct ataaagttag ataagaataa gctaacaagc agaagtaaga
       61 taggtcttgc aagaatggta aaaatctgat aaactaagta gtaattgaat aggaatctga
      121 aagactagta gcccaaggga agtatatcag ggaggagagc cgtgactgca agctctctat
      181 atgaaagctg ggtgaattca cttgcgcatg gatttagaaa tgaaggtctg acttgtcaga
      241 tgaaaacgga tggtaccgcg tgtcaacgct ccgagtggag tttttggcat gtggttttct
      301 ttttatctac gagagactga tggaggaaat atgtcaacta ttgaagaaca attaaaagcg
      361 cttcgcgaag aaacgctggc tagcttgaag cagattactg ctggaaatga aaaagagatg
      421 caagatttgc gtgtctctgt ccttggtaaa aagggttcgc tcactgaaat cctcaaaggg
      481 atgaaagatg tttctgctga gatgcgtcca atcatcggga aacacgtcaa tgaagctcgt
      541 gatgtcttga ctgctgcttt tgaagaaaca gctaagctct tggaagaaaa gaaagtcgcg
      601 gctcaactgg ctagcgagag tatcgatgtg acgcttccag gtcgtccagt tgcgactggt
      661 caccgtcacg ttttgacaca aaccagtgaa gaaatcgaag atatcttcat cggtatgggt
      721 tatcaagtcg tggatggttt tgaagtggag caagactact ataactttga acgtatgaac
      781 cttccaaaag accacccagc tcgtgatatg caggatactt tctatatcac tgaagaaatc
      841 ttgctccgta cccacacgtc tccagttcag gcgcgtgcta tggatgccca tgatttttct
      901 aaaggtcctt tgaagatgat ctcgccaggg cgtgtcttcc gtcgcgatac ggacgatgcg
      961 acccacagtc accaattcca ccaaatcgaa ggcttggtag ttgggaaaaa tatctctatg
     1021 gctgatcttc aaggaacgct tcagttgatt gtccaaaaaa tgtttggtga agagcgtcaa
     1081 attcgtttgc gtccatctta cttcccattc acagagccat ctgttgaggt ggatgtttct
     1141 tgcttcaagt gtggtggaga aggctgtaat gtatgtaaga aaacaggttg gatcgaaatt
     1201 atgggggccg gtatggttca cccacgtgtc cttgaaatga gtggtatcga tgcgactgta
     1261 tactctggct ttgcctttgg tcttggacaa gagcgtgtag ctatgctccg ttatggaatc
     1321 aacgatatcc gtggattcta ccaaggagat gtccgcttct cagaacagtt taaataatga
     1381 ttagaaaagt agaaatggca gatgttgagg tgttggctaa aattgccaaa caagcctttc
     1441 gtgaaacctt tgcgtatgat aatacggaag agcagttaca ggaatacttt gaagaggctt
     1501 atagtctgaa aactttgtca actgagttgg gaaatcctga ctctgaaacc tatttcatta
     1561 tgcatgagga ggagatagct ggttttctca aagtcaactg gggaagtgct caaactgaga
     1621 gagaattaga ggacgctttt gaaattcaac gcctctatgt gctacaaaaa ttccaaggat
     1681 ttggactagg taagcaactg tttgaattcg cacttgaact tgctacaaaa aatagttttt
     1741 cttgggcttg gctaggtgtt tgggagcata atacaaaagc tcaagcgttt tataaccgat
     1801 atggttttga aaaatttagc caacatcatt ttatggttgg tcaaaaagta gatacggatt
     1861 ggttactgag aaagaaatta aggtaagaag agaattcttc tattgaaatg ataggaaagg
     1921 aaaagaacca gtgtggcaat ggtcattctg gagaactaaa ttatgcttgt atcttataaa
     1981 tggttaaaag aattggtggg cattgatgtg ccatcacagg agttggctga aaaaatgtca
     2041 accacaggga tcgaggtcga aggtgtcgaa tcaccagctg ccggtctttc aaaaattgtc
     2101 gtcggtgaga tcttgtcttg cgaagatgtg ccagaaactc accttcatgt ttgtcaggtt
     2161 aacgttggcg aagaagaaga gcgtcagatc gtttgtggtg ccccaaatgt gcgtgcaggc
     2221 atcaaggtta tggtggctct tccgggagct cgtatcgctg acaattacaa aatcaaaaaa
     2281 ggaaaaatcc gtggcttaga gtcacttgga atgatctgtt cacttggtga attgggtatt
     2341 tctgactcag ttgtacctaa gaaattcgca gatggtattc aaattttgcc agaaaatgcc
     2401 gttccaggtg aggaagtctt ttcatatcta gacttggatg atgaaatcat cgaactttct
     2461 atcacaccaa accgtgcaga tgccctttct atgcgtggag tggctcacga agtggcagcc
     2521 atatatgaca aggcagtcaa ctttaaagaa tttactttga cagaaacaaa tgaagctgcg
     2581 acagatgccc tttctgtggg cattgagaca gacaaggcgc cttactatgc agctcgtatc
     2641 ttggacaatg tgactatcgc accaagtcca caatggttgc aaaaccttct catgaacgaa
     2701 ggcatccgtc ccatcaataa cgttgtagac gtgacaaact acatcctgct ctactttggt
     2761 caacctatgc atgcttttga cttggacaca tttgaaggga ctgacatccg tgtgcgtgaa
     2821 gcgcgtgctg gtgaaaaatt ggtgaccttg gacggtgaag aacgtgactt ggacgtgaat
     2881 gacctagtca tcactgttgc agacaagcca gtagcccttg caggtgtcat gggtggtcaa
     2941 gcaacagaaa tctctgaaaa atctagtcat gttgtccttg aagctgctgt tttcaatggc
     3001 aaatctatcc gtaagacaag tggtcgcctg aaccttcgtt ctgagtcatc ttctcgcttt
     3061 gaaaaaggaa ttaatgtggc aacagttaat gaagcccttg atgcggcagc tagcctgatt
     3121 gcggaacttg caggtgcgac ggtgcgtaag ggtatcgttt cagcgggtga gcttgatact
     3181 tcagatgtag aagtttcttc aactcttgct gatgttaacc gtgtcctcgg aactgagctg
     3241 tcttatgctg atgtcgaaga cgtcttccgt cgtcttggct ttggtctttc tggaaatgca
     3301 gacagcttta cagtcagcgt accacgtcgt cgttgggata tcacaatcga agctgatctc
     3361 tttgaagaaa tcgctcgtat ctatggatat gaccgcttgc caaccagcct tccaaaagac
     3421 gatggtacag ctggtgaatt gactgtgaca caaaaactcc gccgtcaagt tcgtacca
//
LOCUS       DAVBHS010000171         3427 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVBHS010000171 DAVBHS010000000
VERSION     DAVBHS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.171"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            161..2794
                     /gene="polA"
                     /locus_tag="VQ651_001905"
     CDS             161..2794
                     /gene="polA"
                     /locus_tag="VQ651_001905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET2754854.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            2879..3316
                     /locus_tag="VQ651_001906"
     CDS             2879..3316
                     /locus_tag="VQ651_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET2754855.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
BASE COUNT          929 a          596 c          875 g         1027 t
ORIGIN      
        1 gtgtactatt atagattcaa tatactatat tatagcacat ttttaaatct aggtttacta
       61 aattctctgg ctctatctat ttattcggaa aaagtttgaa aaatacttgc tctagctctt
      121 cccaatggta ttttttgatt ctttccttta taatgggtgt atggataaga aaaaattatt
      181 attgattgat gggtcttctg tagcttttcg ggcgtttttt gcgctgtatc agcagttgga
      241 ccgttttaag aatgcggctg gtttgcatac caatgcgatt tatggttttc agttgatgtt
      301 gagtcatttg ttggagcggg ttgagccgag tcatattttg gtggcttttg atgcgggaaa
      361 gacgaccttc cggacagaga tgtatgcgga ctataagggt ggtcgggcca agactcctga
      421 tgagtttcgt gagcaatttc ctttcattcg tgagttgctg gatcatatgg ggattcgtca
      481 ctatgagctg gctcagtatg aggcggatga catcattggg acgttggata agctagcaga
      541 gcaggatggt tttgatatta ccattgtcag tggggacaag gatttgattc agctgacgga
      601 tgagcatacg gtggttgaaa tttccaagaa aggtgtggct gagtttgagg cctttacgcc
      661 agattacctc atggaagaaa tgggcctcac accagctcag tttatcgatc tcaaggcgct
      721 catgggtgat aagtcggata atatccctgg ggtgaccaaa gtcggtgaaa agacgggtat
      781 taagctcttg ctggagcatg gttcgcttga ggggatttat gaaaatattg atggaatgaa
      841 gacttctaag atgaaggaaa atctcatcaa tgacaaggaa caggcctttt tgtctaaaac
      901 actagcgacc attgatacca aggcaccgat tgcgattggt ttagaggact tggtctatag
      961 tggtccagat gttgaaaatc ttgggaaatt ttacgatgag atgggattca aacagctcaa
     1021 gcaggcttta aatgtgtcgt cagctgatgt ggctgagggt ttggatttta ctattgttga
     1081 ccaaatcagt caagatatgc tgagtgaaga gtctatcttc cactttgagc tttttggtga
     1141 gaattatcat acggataatt tggttggatt tgcctggtct tgtggggata agctctatgc
     1201 cacagacaag cttgagctgt tgcaagaccc gattttcaag gatttcttag aaaaaacatc
     1261 tctgagagtt tatgacttta agaaggttaa agttcttttg caacgttttg gtgtggattt
     1321 gcaggcgcct gcttttgaca tccgtttggc taaatacctc ctttcgactg tggaggacaa
     1381 tgaaattgcg accatcgcta gtctttatgg tcagacttac ttggttgatg atgaaacttt
     1441 ctacggtaag ggtgttaaaa aggccattcc tgaacgtgag aaattcttgg aacacttagc
     1501 ttgtaaactt gctgttttgg tagaaacaga gcctatttta cttgaaaaac tcagcgaaaa
     1561 tgggcaatta gagcttcttt atgatatgga gcaacctctg gcttttgttc ttgccaagat
     1621 ggaaattgct gggattgtgg tcaagaaaga gaccttgctt gagatgcagg ctgaaaatga
     1681 gcttgtcatt gaaaaactga ctcaagagat ttacgagctg gctggtgagg agtttaatgt
     1741 caactcgcct aagcagttgg gcgtgcttct ctttgagaaa ttgggacttc ctctagaata
     1801 cactaagaaa accaagacag gttattcgac agcagtggat gtgttggagc gtctcgctcc
     1861 tattgctccg attgttaaga aaatcctgga ttaccgtcaa attgctaaga ttcaatctac
     1921 ttatgtaatt ggcttgcagg actggatttt ggctgatgga aagattcata ctcgctatat
     1981 gcaggatttg acccagaccg ggcgtttgtc tagtgtggat ccaaacttgc aaaatattcc
     2041 tgcccgattg gaacaagggc gcttgattcg gaaggctttt gtgccagagt gggaggatag
     2101 tgtgctactc agctctgact attcacagat tgaattgcgc gttttggcgc atatttctaa
     2161 ggatgagcac ttgattaagg ccttccaaga gggggcagat atccatactt cgacagccat
     2221 gcgggtcttt ggcattgagc gtcctgataa tgtgactgca aatgatcgtc gcaatgccaa
     2281 ggcagttaac tttggagtgg tttatgggat ttcagacttt ggcttgtcta ataatttggg
     2341 aattagtcgt aaggaagcca aagcctacat tgatacctac tttgaacgtt ttccaggtat
     2401 taaaaactac atggatgaag tggtgcggga ggcgcgtgat aagggctatg tagagaccct
     2461 ctttaagcgt cgccgtgagt tgccagatat caattcgcgc aacttcaata ttcgtggttt
     2521 tgcggagcga actgctatca actcacctat ccagggttcg gcagcagata ttctcaagat
     2581 tgccatgatt cagctggata aagccttggt tgcaggtggt tatcagacta agatgctgtt
     2641 acaagtgcac gatgaaatcg tccttgaagt gcctaaatct gaattggtag agatgaaaaa
     2701 attggtgaaa caaaccatgg aagaagccat tcaactcagt gttcctctta tcgcagatga
     2761 gaatgaaggg gcaacctggt acgaggctaa ataaaaaggg ggctagtcct ccttttttgt
     2821 agtagaattc tgcaaacctt ttcaaaatgt gctatactga tgaaaaagga ggttttctat
     2881 gagtcaagaa tttatcaatc caagtgatgg cgtgattcgt cagtatctcg caacgagtaa
     2941 aacccttgct gtggtgggtt tgtctgaccg tgaagaaaca accagcaatc gagtgacaaa
     3001 ggaaatgcag gctcggggct ataaaatcat cccagtcaat cccaaggcgg caggtggcga
     3061 aatcttgggt gaaaaggctt atgctagcct cgctgaaatt ccttttcctg tagatattgt
     3121 caatgtttat cgtcgcagtg aatttctgcc cgatgtggcg cgtgattttc tcaaagctga
     3181 tgccaagatt ttttgggcac agctaggact tgaaagttta gaagcgaaag agattttgcg
     3241 tgatggtgga tgtgatgata tcgtgatgaa tcgctgtatc aagagagaac atactcgctt
     3301 gattgaggaa gcataagaaa aaggtagctg atgggctacc ttttgtgtta tactcaatga
     3361 aaatcaaaga gcaaactagg aaactagtcg cgggctgctc aaaacactgt tttgaggttg
     3421 tagatac
//
LOCUS       DAVBHS010000172         3421 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVBHS010000172 DAVBHS010000000
VERSION     DAVBHS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.172"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            93..551
                     /locus_tag="VQ651_001907"
     CDS             93..551
                     /locus_tag="VQ651_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HET2754856.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            564..1172
                     /locus_tag="VQ651_001908"
     CDS             564..1172
                     /locus_tag="VQ651_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754857.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            1243..1575
                     /locus_tag="VQ651_001909"
     CDS             1243..1575
                     /locus_tag="VQ651_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754858.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            1586..2995
                     /gene="ply"
                     /locus_tag="VQ651_001910"
     CDS             1586..2995
                     /gene="ply"
                     /locus_tag="VQ651_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HET2754859.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQHEKITAHSMEQ
                     LKVKFGSDFEKIGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLRQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFESLIKGVAPQTEW
                     KQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFL
                     RDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVLTP
                     KAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRT
                     ISIWGTTLYPQVEDKVEND"
BASE COUNT         1055 a          605 c          772 g          989 t
ORIGIN      
        1 agtctaataa gttatagtcc agtttttact atactttgat attcaaatat gttatactat
       61 ggttatcaaa gtaaatgaaa ggagttacga atatgacgcc agaagaaatg tacctgacag
      121 agcgattaga cgtacagata gctcattttt taaagaaaag cgttcaacat cgtaggcgct
      181 ataaggtatt aaaaataaca gaaatcgtgg caggtttcct catagctgtc ttttgtgcta
      241 ttcctatgcc aggtgatcgc taccgtttga tttcggttgc cttatccagt ctcggcttgc
      301 tgtgtgaggg gattatcaat ttgtataatg caaaggaaaa ttggatttct taccaaaaaa
      361 ctgcgcaact cctggagaaa gaaaaattcc tctatcaatg ccaaacggag aaatatgcag
      421 gaaagaccaa ggcttttgcc ctatttgtca agacatgcga aggtcttatc tcagaggaga
      481 ttaaccagtg ggaaagtatc cagtcaaaag aagtggcagc tagtgcagat gctccagtta
      541 aaaaagagta ggaggtagag gaaatgtctc aatccagcta cctgtcgccc ttgctctggt
      601 taaaaaaaga agccgataag gaaaagatga gcgcgaccca gtgccagata tttttctttt
      661 actatcaaat gtttgagctc ttatttgcta gagaaagcga catgaaagac ttatgtctgg
      721 gaacgaaagg tttttatttc tcgcagttag agaaaaattt gctttctgga gtttcccgat
      781 ttctaaaaaa cttggagggg aaagtaactc tcaaggctaa ccaagaagta tcagctcgca
      841 aagccctttt tctagccttg acaactagcc aatcagattg gcaggagtta gctcctgttt
      901 ttgattttta tcagactatc gggaggcttg aaaatccttc tctcttgagt tctcaggaca
      961 gacaacatct gatgtggatt taccagtcag ctttggagaa ggattatatt gtcaaggtta
     1021 ttggcgacaa gcattttgta ttgaagagac aagatgctac taaattgaca gcgcgccaaa
     1081 ctcaaacttt ggaaattctg agtcaatcag aagacttggt caatcctgtc tatgttacat
     1141 taggagaaaa gggggtgctc ttgcttgatt aagagaggag atgttgtagc tctttatttg
     1201 ccttttccga ctattagtag cgatttggct gtgaagaatc atatgtatat ctgtattgac
     1261 aacagcatga ctaaaaacaa agagttggtt aaaaatcaga ccttcaaacc agctctcttg
     1321 accagacgtt tggtcaagaa ctttatgata gaagagccgg atctagctcg taatcctttt
     1381 acaagaccaa ccttgattga cttagataag gtatttatgt tggataatac ggtcattccg
     1441 acttcttatt tagccagacg gcgacgcaat gtctcagaag aattgtacga ggaaattttg
     1501 gattacttag tccaaccacg gctgatttcg ctgaacaagt ctgagtttat gcaactcaat
     1561 ccaggaactt attaggaggt agaagatggc aaataaagca gtaaatgact ttatactagc
     1621 tatgaattac gataaaaaga aactcttgac ccatcaggga gaaagtattg aaaatcgttt
     1681 catcaaagag ggtaatcagc tacccgatga gtttgttgtt atcgaaagaa agaagcggag
     1741 cttgtcgaca aatacaagtg atatttctgt aacagctacc aacgacagtc gcctctatcc
     1801 tggagcactt ctcgtagtgg atgagacctt gttagagaat aatcccactc ttcttgcggt
     1861 cgatcgtgct ccgatgactt atagtattga tttgcctggt ttggcaagta gcgatagctt
     1921 tctccaagtg gaagacccca gcaattcaag tgttcgcgga gcggtaaacg atttgttggc
     1981 taagtggcat caagattatg gtcaggtcaa taatgtccca gctagaatgc agcatgaaaa
     2041 aatcacggct cacagcatgg aacaactcaa ggtcaagttt ggttctgact ttgaaaagat
     2101 agggaattct cttgatattg attttaactc tgtccattca ggcgaaaagc agattcagat
     2161 tgttaatttt aagcagattt attatacagt cagcgtagat gctgttaaaa atccaggaga
     2221 tgtgtttcaa gatactgtaa cggtagagga tttaaggcag agaggaattt ctgcagagcg
     2281 tcctttggtc tatatttcga gtgttgctta tgggcgccaa gtctatctca agttggaaac
     2341 cacgagtaag agtgatgaag tagaggctgc ttttgaatct ttgataaaag gagtagctcc
     2401 tcagacagag tggaagcaga ttttggacaa tacagaagtg aaggcggtta ttttaggggg
     2461 cgacccaagt tcgggtgccc gagttgtaac aggcaaggtg gatatggtag aggacttgat
     2521 tcaagaaggc agtcgcttta cagccgatca tccaggcttg ccgatttcct atacaacttc
     2581 ttttttacgt gacaatgtag ttgcgacctt tcaaaacagt acagactatg ttgagactaa
     2641 ggttacagct tacagaaacg gagatttact gctggatcat agtggtgcct atgttgccca
     2701 atattatatt acttgggatg aattatccta tgatcatcaa ggcaaggaag tcttgactcc
     2761 taaggcttgg gacagaaatg ggcaggattt gacggctcac tttaccacta gtattccttt
     2821 aaaagggaat gttcgcaatc tctctgtcaa aattagagag tgtaccgggc ttgcctggga
     2881 atggtggcgt acggtttatg aaaaaaccga tttgccacta gtgcgtaagc ggacgatttc
     2941 tatttgggga acaactctct atcctcaggt agaggataag gtagaaaatg attaggagag
     3001 aagaatgttt gcgacaaaaa gaggcgatga gctctctgcg aatatctgaa agtttatctc
     3061 ttgcctagcg atttccattg gagtatgctc gtgatgttaa aatgagtcta tcaaacagga
     3121 tgtgtaaaaa agtaaaaagg gtagccaatt ccttgatata caggggattg attatccttt
     3181 acttgtttta gatgattgga aattcaagaa tgacacgtat cggaaatatt ttgctgttta
     3241 tatctaatac ccatttatgg aaaaaatgaa attaaatatg aacaaatcaa ttgctagcaa
     3301 tggtttagaa gtgccagtgt actagtctaa cttctccatc ttaggaacac gtaaattata
     3361 ctcttcgaaa atctcttcaa accatgtcag ccctatccgc aacctcaaaa cagtgttttg
     3421 a
//
LOCUS       DAVBHS010000173         3393 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVBHS010000173 DAVBHS010000000
VERSION     DAVBHS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.173"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            58..927
                     /locus_tag="VQ651_001911"
     CDS             58..927
                     /locus_tag="VQ651_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET2754860.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            931..1887
                     /locus_tag="VQ651_001912"
     CDS             931..1887
                     /locus_tag="VQ651_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET2754861.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            1887..2651
                     /locus_tag="VQ651_001913"
     CDS             1887..2651
                     /locus_tag="VQ651_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754862.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            2651..3361
                     /locus_tag="VQ651_001914"
     CDS             2651..3361
                     /locus_tag="VQ651_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754863.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKIPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
BASE COUNT          919 a          630 c          752 g         1092 t
ORIGIN      
        1 aacctatcaa aaagtgaggg aaaaccctcg gaattataaa tagaaagagt gaatcttatg
       61 ctccaacaac tcgtaaatgg tttgattcta ggtagtgttt acgcgctgtt agccctagga
      121 tataccatgg tttacggaat tatcaagctc atcaacttcg cccatggtga tatttatatg
      181 atgggagcct ttatcggtta tttcttgatc aattctttcc aaatgaattt ctttgtagcg
      241 cttattgtag ctatgctagc gacagctatt cttggtgtcg tgattgagtt tcttgcttac
      301 cgacctttgc gccactctac tcgtattgct gttttgatta cggctattgg ggtttctttc
      361 ctattggagt atggaatggt ctatctggtt ggtgccaata cccgtgcctt ccctcaagcg
      421 attcaaacag ttcgatatga tttgggacca attagcttaa caaatgtgca gttaatgatt
      481 ttggccattt ccttgatttt gatgattttg ttacaagtca ttgtccaaaa gactaagatg
      541 gggaaagcca tgcgtgcagt atcagtagat agcgacgcgg cgcaattgat ggggatcaat
      601 gtaaaccgta cgattagctt taccttcgct ttgggttctg ctcttgcggg tgcggctggt
      661 gttctgattg ctctttatta taactctctt gagcctttga tgggggttac tccaggtctt
      721 aaatctttcg ttgccgcagt acttggtggt atcggaatta ttcctggtgc ggctcttggt
      781 ggctttgtga ttggtctatt ggaaaccttt gcgactgcct ttgggatgtc agatttccgt
      841 gatgccattg tttatggaat cttgttgttg atcttgattg tccgcccagc tggtatcctt
      901 ggtaagaatg tgaaagagaa ggtgtaaacg atgaaggaaa atttaaaagt taatattcta
      961 tggttactcc ttttgttagc tggctatagc ttgattagtg tactggtttc agtcggagta
     1021 cttaatctat tctatgtaca gattttacaa caaattggaa ttaatattat tttggctgtt
     1081 ggtctcaact taatcgttgg tttttcagga caattttcac ttggtcatgc tggtttcatg
     1141 gcgattggtg cctatgcagc agctattatt ggttctaaat taccaaccta cggtgccttc
     1201 tttggagcta tgcttgtagg ggctttgctt tcaggagcag ttgccttact tgtcggcatt
     1261 ccaaccttgc gcttgaaggg ggactatctt gcggtagcaa ctctaggtgt ttctgaaatt
     1321 atccgtatct ttatcatcaa tggtggaagt cttacaaatg gtgcggcagg tatcttaggg
     1381 attcctaact ttacaacttg gcaaatggtt tacttctttg tcgtgattac aaccattgca
     1441 accttgaact tcttgcgtag cccaattggc cgttcaaccc tctctgttcg tgaagatgaa
     1501 atcgctgctg agtcagttgg ggttaatacg actaaaatta aaatcatcgc ttttgtcttt
     1561 ggtgccatta ctgcaagtat tgctgggtca cttcaggcag gatttatcgg gtctgttgta
     1621 ccgaaagatt acaccttcat taactcaatc aacgttttga ttattgttgt atttggtgga
     1681 ctcggttcca ttacaggtgc gattgtttcg gctattgttc tgggaatttt gaatatgctt
     1741 ctccaagatg ttgctagtgt gcgtatgatt atttacgctt tagccttggt attggtaatg
     1801 attttcagac caggtggact ccttggaacg tgggaactga gcctatcacg tttctttaaa
     1861 aaatctaaga aggaggaaca aaactaatgg cattacttga agtaaaacag ttaaccaaac
     1921 attttggtgg tctaacagct gttggagatg tgactcttga attgaacgaa ggggaactgg
     1981 ttggattaat cggtccaaac ggagctggga aaaccaccct tttcaacctt ttgaccggtg
     2041 tttatgaacc aagcgaggga acagtaaccc tagatggtca ccttttgaat gggaaatcac
     2101 cttataagat tgcctctttg ggacttggac gtactttcca aaatatccgt ctctttaaag
     2161 atttaacagt tttagataat gttttgattg cttttggaaa ccatcacaaa cagcatgttt
     2221 ttactagttt cttacgctta ccagcttttt acaagagtga aaaagaatta aaggctaaag
     2281 ctttggaatt gttgaaaatc tttgatttag atggtgatgc agagactctt gctaaaaatc
     2341 tttcctacgg acaacaacgt cgtttggaaa ttgttcgtgc ccttgctacg gaacctaaaa
     2401 ttctcttctt agatgaacca gcagcaggta tgaacccaca ggaaacagcc gaattgactg
     2461 agttaattcg tcgtatcaaa gatgaattta agattacaat catgttgatt gaacacgata
     2521 tgaatctggt catggaagta acagaacgta tctacgtact tgaatatggc cgtttaattg
     2581 ctcaaggaac tccagacgaa attaagacca ataaacgcgt tatcgaagct tatctaggag
     2641 gtgaagccta atgtctatgt taaaagttga aaatctttct gtgcattacg gtatgatcca
     2701 agcagtccgt gatgtaagct ttgaagttaa tgaaggagaa gttgtttccc ttatcggtgc
     2761 caacggtgca ggtaagacaa ctattcttcg caccttgtca ggtttggttc gaccaagttc
     2821 aggaaagatt gaatttttag gtcaagaaat ccaaaaaata ccagctcaga aaatcgtggc
     2881 aggtggtctt tcacaagttc cagaaggacg ccacgtcttt cctggcttga ctgttatgga
     2941 aaatcttgaa atgggagctt tcttaaagaa aaatcgtgaa gaaaatcaag ctaacttgaa
     3001 gaaggttttc tcacgctttc ctcgtcttga agaacgtaag aaccaagatg cagctactct
     3061 ttcaggaggg gaacaacaaa tgcttgccat gggacgcgct cttatgtcaa caccaaaact
     3121 tcttctttta gatgaaccat caatgggact tgccccaatc ttcatccaag agatttttga
     3181 tatcattcaa gatattcaga agcaaggaac aaccgtcctc ttgattgaac aaaatgccaa
     3241 taaagcactt gcaatctctg accgaggata tgtattggaa acaggaaaaa tcgtcctatc
     3301 aggaacagga aaagaactcg cttcatcaga agaagtcaga aaagcatatc taggtggcta
     3361 aaacaatcca gtggattgtt ttagtcggca gat
//
LOCUS       DAVBHS010000174         3279 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVBHS010000174 DAVBHS010000000
VERSION     DAVBHS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.174"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            2..1242
                     /locus_tag="VQ651_001915"
     CDS             join(2..341,341..1242)
                     /locus_tag="VQ651_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2754864.1"
                     /translation="MVKLIDRYGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSIS
                     LDYALPNQGMLSFWPAQYKKNGYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENE
                     RLRTEVAYLKKFKRVRGKGRSPRARKAETVREMVSGGFRLDFLLETARLARSTYYYQL
                     KQLDGVDKDKEIKTEIQGIDNEHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLT
                     ARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPV
                     LDGFNSEIIAYHLSTSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLE
                     SKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNN
                     KRIKVKLKGLSSVQYRTKSFG"
     gene            complement(1477..2085)
                     /locus_tag="VQ651_001916"
     CDS             complement(1477..2085)
                     /locus_tag="VQ651_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HET2754865.1"
                     /translation="MQIKFIDKVSNLIMLNLLYVASVVTVIAIGSGESALIATLIKIV
                     RHEESYPYRDFANSFFKDYWKNLGAALISNLPILILLFSLFFLPYIPLPIYIISILRH
                     IGVIYIILHLIATTFLIPLIGRYNNTLKNSLHNSIMLAYKHFFIAVLIRIIEIIPVLL
                     FFILQNQLLVWITLMIFILPSITKYANAFLYNFIFSKYEKLN"
     gene            2251..2421
                     /locus_tag="VQ651_001917"
                     /pseudo
     CDS             2251..2421
                     /locus_tag="VQ651_001917"
                     /inference="COORDINATES: protein motif:HMM:NF013577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            2410..3021
                     /locus_tag="VQ651_001918"
     CDS             2410..3021
                     /locus_tag="VQ651_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2754866.1"
                     /translation="MTKDILELESQKMSSDTFIDEIKNNYLSIVESTRKLIDGRQIEL
                     AIKLIREANQILMIGVGSSGNAAREFESSLLRIGIISKTVIDTHFQLMHTALLKDNDL
                     IIAFSLSGSTKEVEETLLNAKRKNVKIISITNYSSRNIAKLSDCVLLTSKKESYLEGG
                     SLMAKASQLFIIDVICTRLSLINYEDTICKKEEIASLLSNKVE"
BASE COUNT         1239 a          420 c          588 g         1032 t
ORIGIN      
        1 catggtgaaa ttaattgacc gttatggcat agaaatcgtt aaaaaaggga agaatcgtca
       61 ctattcttct aaattgaaac aagaaatgat ggataaagcc ttactggaag gttgttcaca
      121 aagaagtatt agtcttgatt atgccctccc aaaccaagga atgctttcat tttggccggc
      181 acaatacaag aaaaacgggt atactattgt tgagaaaaca agagggagac cagctaaaat
      241 gggacgtaaa cggaagaaga cttgggaaga aatgacagaa ctagaacgac tccaggagga
      301 gaatgaacgc ttacggactg aggtggccta cctaaaaaag ttaaaagagt tagaggaaag
      361 ggacgaagcc ctagagcgag aaaggcagag acagttagag aaatggtttc aggaggattt
      421 cgactagatt ttcttcttga aacagcacgt ttagctcgct cgacttacta ttatcagttg
      481 aaacaactgg atggggttga taaagataaa gagattaaaa ccgaaattca gggcattgat
      541 aatgaacata aaggcaacta tggctatcgt cgaatccatt tagaactaag gaatcgtggt
      601 tttgtggtca atcataagaa agttcaacgg ttgatgagaa ttcttggttt aacggctcga
      661 attcgccgta aacggaagta ttcttcctac caaggagaga ttggcaagaa agcagagaat
      721 ctcattcaac gccagttcga agcatcgagg ccaatggaaa agtgctatac agatgtgaca
      781 gagtttgcca ttccaaatag cacacagaaa ctctatttat caccagtttt agatggtttt
      841 aacagtgaaa tcattgctta tcatctttct acttcgccaa acttagaaca agtgaagagt
      901 atgttagaac aggcctttac agagaaatac tatgagaata cgattctcca tagtgaccaa
      961 ggctggcaat accaacacga ttcttatcat cggttcctag agagtaaggg aattcaagca
     1021 tccatgtcac gcaagggcaa cagcccagac aacggcatga tggaatcttt ctttggcatt
     1081 ttaaaatccg aaatgtttta tggctatgag aagaacttta gatctttaga aaaccttgaa
     1141 caagctattg tggactacat tgattactac aacaacaaac gaattaaggt aaaactaaaa
     1201 ggacttagtt ctgtgcagta cagaactaaa tcctttggat aaattatttg tctaattttt
     1261 tggggtcagt acagtattaa ataccatgat cttatgcgtt ttataataaa aaatctgttc
     1321 ttgttttatt tttagaaaat ttactaaaga ctaaaaatat tatgaataat ttaaaaaata
     1381 gtcaggttat atagtttttt gaaattaaaa ttattttatc gttgaaatta gttaaaaatt
     1441 tttataaaat caagatgcaa ggctatgaga tgttctctag tttaattttt catacttcga
     1501 gaatataaag ttatataaaa atgcattagc atattttgtt attgagggaa gtataaaaat
     1561 cattaaagtt atccaaacta gtaattgatt ctgtaatata aagaagagta gaactgggat
     1621 aatttcaata attctaataa gaacggcaat aaaaaaatgt ttataggcta acatgattga
     1681 attgtgtaga gaatttttta aagtgttatt atatctaccg attaaaggaa ttagaaatgt
     1741 tgttgctatt aaatgaagta tgatatatat tactccaata tgtcgtaaga tagaaatgat
     1801 atatataggt aatggtatgt agggtaggaa gaacaaactg aataataaga ttaaaattgg
     1861 taaattagat attaatgctg ctcctaaatt tttccaataa tctttaaaaa aactgtttgc
     1921 gaagtctcta tagggatagg attcctcgtg tcgtacaatt tttattagag ttgctattag
     1981 ggcactttca cctgatccga tagcaataac tgttactact gacgctacat agagtagatt
     2041 taacattata agatttgaaa ctttatcaat aaattttatc tgcatcttta actcctgctg
     2101 tttttatact tataatatca catcatttga gtttcatcaa ctttgaatac attaagaaag
     2161 aatataacta tgaggaaagg aaaaaattat tatgaataaa tccaaaaaat ataaagaaag
     2221 aattcattat gaatacgata atcttactaa atcagagaaa aaattgctga ctatatttta
     2281 gttagagata gtgaaatagt ttatgaaacg tcttcagaaa ttgttagtaa agtaaaagtt
     2341 ggagagggaa ctcttaatcg attttgcaaa aagattggat actctggttt tcaggaacta
     2401 aaattaaaaa tgacgaaaga tatattagag ttagaatctc aaaaaatgag tagtgacaca
     2461 tttattgatg aaataaaaaa taattattta tctattgtgg aaagtacaag aaaattaatt
     2521 gatggtagac aaatagaatt agctataaaa ttaataagag aagcaaacca gattttaatg
     2581 attggtgtgg gttctagtgg aaatgcggct agagaatttg agagtagttt gttaagaatt
     2641 ggaataataa gtaagacggt aattgatacc cattttcaac tcatgcatac tgcgttattg
     2701 aaagataatg atttgataat agcgtttagt ttatctggtt ccacaaaaga agttgaggaa
     2761 actttattaa acgcaaaaag aaaaaatgtt aaaattattt caataacaaa ttattcgtcc
     2821 agaaatatag caaaattatc tgactgcgtt cttttgacat caaaaaaaga atcttattta
     2881 gagggtggta gtttaatggc taaggcttct caactattca taattgatgt aatatgcact
     2941 agactctctt taataaatta tgaagatact atatgtaaaa aagaagagat tgcttctttg
     3001 ctgtcaaata aagtggaata aatcaataat ttttaaattt taataaaaat tttttaaaat
     3061 aaattaaaaa acgatcctaa aagggtcgtt tttttgctaa agttttaaaa ataaacaata
     3121 attaagatta tagtgcgttg aatctatatt aggacataac aattcttaaa tcattgttag
     3181 aaattagttt gaattttcca atcaatttgt tcgattttta catcattgca ttatatcacg
     3241 tgactattaa ccttgttttc tcctaaaatt gattttctt
//
LOCUS       DAVBHS010000175         3242 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVBHS010000175 DAVBHS010000000
VERSION     DAVBHS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.175"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(196..807)
                     /gene="rpsD"
                     /locus_tag="VQ651_001919"
     CDS             complement(196..807)
                     /gene="rpsD"
                     /locus_tag="VQ651_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET2754867.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1002..2054)
                     /locus_tag="VQ651_001920"
     CDS             complement(1002..2054)
                     /locus_tag="VQ651_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET2754868.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVTKLEKGQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2051..2749)
                     /locus_tag="VQ651_001921"
     CDS             complement(2051..2749)
                     /locus_tag="VQ651_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2754869.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2916..>3242)
                     /locus_tag="VQ651_001922"
     CDS             complement(2916..>3242)
                     /locus_tag="VQ651_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2754870.1"
                     /translation="VKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSS
                     TNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLK
                     NKKEEN"
BASE COUNT          906 a          733 c          624 g          979 t
ORIGIN      
        1 aatatgccat aaaaggatta taccacattg tgtactattt ttggttcatt ttactatatc
       61 actttggact atatcggtct agttatgtct aaacgattat agctggatac taagagaaat
      121 caaaaaagca ctaagggaga acgtcccaaa gtgcttaata tcaaggcctc aaagcctgtc
      181 ttattcaata aaatattaca acatcttgtt gtagaattca acgacaagtg cttcgttgat
      241 ttctgggttg atttcgtcgc gttctggcaa gcgagtcaat gaaccttcca atttttcagc
      301 gtcgaatgat acgaatgctg gacgtccaag agtagcttct actgcttcaa ggattgctgg
      361 aactttcaat gatttttcac gaactgagat cacttgacct ggagttacgc ggtatgatgg
      421 gatatcaacg cgtttcccgt caacaaggat gtgaccgtgg tttacgaatt gacgagcttg
      481 acgacgagta gtcgcgagac caagacggta aacaacgtta tccaaacgac gttccaaaag
      541 aagcataaag ttgaaaccta ggattccgcc tttgattttt gtagcttgta cgaacaagtt
      601 acggaattgt ttttcaccta caccgtaagt gaaacgaagt ttttgttttt cagccaattg
      661 caaaccgtat tctgacaatt tagaacggtt gtttggtccg tgttgtcctg gtacgtagtt
      721 acgacgtgcc aattctttac ctgtacctgt aagtgaaagg ccaaggcgac gagcttgttt
      781 ccaagatggt cctgtataac gtgacatatg aatgtcctcc tgatataaat aatatttcgg
      841 tggaaatagt cacttagaaa gccctgattc gtgcagatgc ccttcgccta aacagccaag
      901 gttacttgtc ataagacacc tgttgacgag cttcatgctt tcctgctgat atttcacaca
      961 aaatccattt taccatgaat agctggattt gtaaaggggt tttaggcttt attttcacta
     1021 ccagagagat tgagaacgag ggtaaaggta cttcctagac cgtactggct gctgactgtg
     1081 atttccccac ccaattgatg ggccaattca cgcgcaatcg caagtcctaa tccatgacca
     1141 cctgtcttca tgttacgcga agtttcgaca cgataaaggc gtttgaaaat attttccaaa
     1201 tcctctgggg caatgccctg cccttcatcg gtcacactga ttgaaagctg gcccttctcc
     1261 agcttagtca ccacttccag cttggttcct ggagcagaat atttaaaagc gttatcgacc
     1321 agattcacca agatacgaga aagcttagca taatctccct caatccgggc agactctggg
     1381 attacctgca agtggacatc tcttctctcc tgctcaatca aaaactgaaa ttcactcatg
     1441 cactcaatta agagcttgtc cagaaaaata ctgtctttac tggtagtttc cacctgattt
     1501 ctagctgtgt ttagggtcaa aaaattcaac tcctcaacca gtttattgag cctctccgtc
     1561 tggcgtccaa tggttgctag ataatgagct tgctccgact ccttgataat cccatccaaa
     1621 atcccttcta ccgtcgcttg gatcgaagtg ataggggtct taatatcatg cgacaactgg
     1681 gcaatcatca agcccttttc tcgttcgctt tcttccaagg aatcaaagct tacctgcaaa
     1741 tcatgggaca tctcattaaa agtttgccct aattgctgaa attctacagg accttgaacc
     1801 tccaaatttg aaggaaaatc cttggccgct acccgcttgg catgctcctt gagtttgccc
     1861 aacgacgtaa agactggcaa taggagaaag agactaatcc cagcaccgac aaggctggca
     1921 acgatggtca tcccaagcaa aaagtaaatc tcgcctttcg caatcagcat tttttgaaca
     1981 gcccaaaaaa caaccaaaat ggttaagagg gttgaaataa tatatccaac caaaatataa
     2041 ctttttagtt tcatgtttgt cctctcggtt tctctatctt atatcccaac ccccaaactg
     2101 tcttaatagt gggcgtttgg tcactactat attttgccag ctcctgtcga agagcatgga
     2161 tatgcacatt caaggtattg gtgtcatcca cgtagtcttc tttccagatc ttttcataga
     2221 ggtctgtctt ggagaaaact cgctctggat tgctagctaa aatccacagc aattcaaatg
     2281 atttaacagt taaatccagc atttcttctc ctatttgaac ttcatgacta ctatgattca
     2341 tttttagatt gccaagggaa atcagctctg tttcgccccc acgatgaagg cggcgcaaaa
     2401 tattgtggac acgcaaaacc agctcacgtg ggctaaaagg cttagcaata aaatcatctg
     2461 ctcccaagct caggccgtaa atcttgtcct gttcactggt cttagcagta ataaatagga
     2521 aaggctgctc tggtgataag tattgaacct cactgattaa atcataacca tccatccgag
     2581 gcatcataac atctgtgata atcaaatcaa tcggtttttt cttgaacagc tctagcgctt
     2641 ccagtccatc atgggctacc aagacctgat aacctgcctg aattaagtat ctctgatgaa
     2701 tatctgtgat ttctacctcg tcgtcaacga gtaaaattgt ctttcccatc tgtctctcct
     2761 ttgataaaaa cagtgctata ctgctttaag tataacacta tttttattgt tttacgatga
     2821 ttttaatctg ctattgaaag aaagtcctga ttgctgattg agttgttaaa ctaatcttag
     2881 tcagcccact atccatcatt tatttcctta gctgattagt tttcttcttt tttgtttttc
     2941 aaacctagac caccgagtaa acctgcaagc gcaagcccaa ggaaaccaat acttgccatt
     3001 gatgtttgag tctcaccagt atttggtagc atagctttat cctctgacat catcgtatca
     3061 gacatcttgt tagcagaagc agccatgttt tcacctgcca tcgtgttggt agaacttgtc
     3121 atggtgtcag caggcatgct atctgtagta cctgtagcat gattgtgatt catcggagtc
     3181 acgccagaac cagagttaga aggtgataat gaaccatttg ctgtgtctga tgtttcttta
     3241 ac
//
LOCUS       DAVBHS010000176         3192 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVBHS010000176 DAVBHS010000000
VERSION     DAVBHS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.176"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(381..914)
                     /locus_tag="VQ651_001923"
     CDS             complement(381..914)
                     /locus_tag="VQ651_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HET2754871.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(1003..1779)
                     /gene="recX"
                     /locus_tag="VQ651_001924"
     CDS             complement(1003..1779)
                     /gene="recX"
                     /locus_tag="VQ651_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HET2754872.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDEETTQELIFKELDK
                     QYTKYARKYEGYKLKQRLTQVLARKGYDFSDIASALREYL"
     gene            1817..3172
                     /gene="rlmD"
                     /locus_tag="VQ651_001925"
     CDS             1817..3172
                     /gene="rlmD"
                     /locus_tag="VQ651_001925"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET2754873.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT         1028 a          562 c          581 g         1021 t
ORIGIN      
        1 atttcatttt actatactgt tacgaaatta gatatataaa tgaaaacttg cctcaaggga
       61 ggttttttgc tagtagaaaa gcgatagaca gaaattgttg gactcttaaa aaaatgaagt
      121 tttgctcaaa agagcttctt aaattaaaac gagcaattat cagttcaaat ctgtttcaaa
      181 agaagaacgg ataaattaat cgatgctttg tttttgagtt aggaagttaa gcatgccaaa
      241 ctgagtcgta ggaataaatt tctttataga aatgtgttgt aactctgcta taatggattt
      301 attccttttt gtgtttacac aatttatttt atactaccaa aaaaacaagg tcaggatttt
      361 gttcctgacc tttgacaact ttaccgattc tttagttcta catagcgctt gtaccaaatg
      421 tttacatagg cttctgagaa aggaccacgt ccattgttaa tccaatcaac aagaattttg
      481 acatgttctt ttaaaatata gtccaagtca tcagaataat tcattttgcg tttgtgacgc
      541 tcgtactctt caacgtccaa gagacgtttt tccccatctg taaaaatttt aacatccaaa
      601 tcgtaatcaa tatacttcag tgcttcttca tccagatagt aggggctagc catattgcaa
      661 tagtaagaag ttccattatc acgaatcatg gcaatgatat taaaccaata tttcttgtga
      721 aagtaaacaa tagccggttc tcgagtgacc caacgacgac catcactttc ggtaacaagt
      781 gtatgatcgt tgacaccaat aatggcgttt tctgttgttt ttagtaccat ggtgtcccgc
      841 caagttcggt ggagactccc atcatgctta taactttgaa ttgtaataaa gtcgccttct
      901 tttggaagct tcataactaa ccaactttct acaatttata agtttatcat ttactattgt
      961 accataaaat tacctaaaat ctgtgaattt cacttggaaa tattaaagat attctctaag
     1021 agcgcttgct atatccgaaa aatcgtagcc ctttcgtgct aaaacttgag ttaaacgctg
     1081 cttcagtttg tatccttcat actttcgggc atacttagta tattgcttat caagttcctt
     1141 gaagatgagt tcctgagtcg tttcttcatc aacttgacta tccaattcgt caaaggcaat
     1201 tttagcatca aaataagaga agcccttgtt agtcaagttc tggataatct tatcttgcag
     1261 ggcacgagct ggaagttttc cctcatattt tttcaatagt ttattggcta cacgttgagc
     1321 aacttccgaa aaatcaaaat cattcaagat ttcttctata gtagattttg aaattccttt
     1381 ttgtgctaat ttctgagtca gtacataagg tcccttgtct cctgaaagtt gattggcatt
     1441 gatgatagca taagcgtact gaccatcatt aatccactta tcttctttaa gattagcaat
     1501 aacttgagaa acgatgtttt tatcaatatc gtattttttc agatattctc tgacttcttt
     1561 ttcagtgcgt gctttaaagg ataagtggta gagggccaga ttcttaccat aagaaaattg
     1621 agcaaagtct tgaatctctt tcaattcctc ttcgcttatc accttatctc tcgataacat
     1681 aaaacgaaca attgtatctt cggtgatata gcatttgtcg ccattatcaa gctccatcag
     1741 atagagtctt tttttctttt caagttttgt gattttcata gctctattat aactcaaaat
     1801 gtgataagat aggggtatga atctgaaagt gaaacaaaaa ataccattaa aaatcaagcg
     1861 catgggaatt aacggtgagg gaatcggctt ttaccaaaaa acattagtct ttgtaccagg
     1921 agctctcaaa ggcgaagata tctattgtca gattacttct attagacgca actttgttga
     1981 agcaaaatta ctgaaggtca acaagaagtc taaatttcga attgtgccat cttgtactat
     2041 ttataatgaa tgcggaggct gccaaatcat gcacctgcat tatgataagc agctggagtt
     2101 caagacggac ttacttcatc aagcgctgaa aaaatttgct cctgcaggat atgaaaatta
     2161 tgaaattcgt ccaactattg gaatgcagga accaaaatat tacagagcta agttacaatt
     2221 tcagactcga aaatttaaaa atcaggtcaa ggcgggctta tatgcacaaa actctcacta
     2281 tttagtagag ttgaaagact gcctggtaca agataaggaa acccaagtga ttgctaatcg
     2341 cttagcagaa ttacttactt atcaccagat tccaatcacg gatgagagaa aagttctagg
     2401 tgtccgtact attatggtcc gacgcgcgag aaagaccgga caggttcaga ttattattgt
     2461 tacaaaccgc cagcttaatt taactcaatt ggtaaaagag ttggttaaag atttcccaga
     2521 agttgtgaca gtagctgtta atacaaatac agctaaaacc agtgagatat atggtgaaaa
     2581 gacagagatt atctgggggc aagagagtat tcaagaaggt gtactcaatt atgaattttc
     2641 actatcccct cgagcttttt atcaactaaa tcctgagcaa acagaagtcc tctatagcga
     2701 agcagtaaaa gcgctggatg ttgataaaga agaccatttg attgacgctt attgtggagt
     2761 tggaacgatt ggatttgcct ttgcaaagaa agtaaaaaca ctcagaggta tggatattat
     2821 tccagaagct attgaagatg ccaagcgaaa tgctaaaaga atgggatttg acaatactca
     2881 ttatgaagct ggaacggcag aagagattat tcctcgttgg tacaaggaag gctaccgagc
     2941 agatgctttg attgttgacc caccacgtac aggtctggat gataagttat tagatactat
     3001 tcttacttat gtaccagaaa aaatggttta tatttcttgt aatgtttcga ccttggctcg
     3061 tgatttggta cgcttagtag aagtctatga tcttcattat atccagtcgg tcgatatgtt
     3121 cccacataca gctcgaactg aagctgttgt aaaattaata aaaaaagttt aaaaaaggtg
     3181 ttgacaaaac at
//
LOCUS       DAVBHS010000177         3057 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVBHS010000177 DAVBHS010000000
VERSION     DAVBHS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.177"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            143..901
                     /locus_tag="VQ651_001926"
                     /pseudo
     CDS             143..901
                     /locus_tag="VQ651_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            941..1705
                     /locus_tag="VQ651_001927"
     CDS             941..1705
                     /locus_tag="VQ651_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2754874.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1718..2395
                     /locus_tag="VQ651_001928"
     CDS             1718..2395
                     /locus_tag="VQ651_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2754875.1"
                     /translation="MIDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILAHIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCMGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2405..>3057
                     /locus_tag="VQ651_001929"
     CDS             2405..>3057
                     /locus_tag="VQ651_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2754876.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNTVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLCILIALIYFSFNLAISSLSHMLAKRWQQAA
                     "
BASE COUNT          839 a          731 c          583 g          904 t
ORIGIN      
        1 attgttagaa atcgatttga ctgtcccgat cgatttgtcc tgttcttatt tcactatagt
       61 gaattttttc agatatggtt tgacaagaac attctttcat tgtagaatcg tgttataaaa
      121 tataacacaa aggagatctc tcatggcttt agtagaattt aaaaacgtcg aaaaatatta
      181 cggagactac cacgcactcc gcaacatcaa tctccgtttt gaaaaaggac aagttgttgt
      241 cctgcttgga ccttctggct ctgggaagtc cactcttatc cgtacgatca atggtttaga
      301 ggctgttgac aaaggaagtc tcctagtcaa tgggcaccaa gttgctggtg ccagccagaa
      361 agatttggta cctcttcgca aggaagtcgg catggttttt caacatttta acctttatcc
      421 acacaaaacg gtgttagaaa acgtgacact tgcgcccatt aaagttctag gaattgataa
      481 aaaagaagct gaaaaaaccg cccaaaaata tctggaattt gtaaatatgt gagacaagaa
      541 agattcctat cccgccatgc tatctggtgg acaaaaacag cggatcgcca tcgctcgtgg
      601 tcttgctatg catccggaac tcctcctctt tgatgaacca acatctgctc ttgatcctga
      661 gactatcgga gatgttctag cagttatgca gaaactggcg catgatggga tgaacatgat
      721 catcgttacc cacgaaatgg gctttgctcg agaggttgcg gaccgcatta tctttatggc
      781 cgacggagaa gttttagtag atacgacaga tgtcgataac ttttttgaca atccaagcga
      841 acctcgtgcc caacaattcc tcagcaaaat tatcaaccac gaaagtgaca aagtcaaata
      901 aggaggcgcc tatgaaaaaa aattcttttt atcagcatta ttgattagcc ttttcggcct
      961 tgctgctgcc aaaccagtcc aggctgatac tagtatcgca gacattcaaa aaagaggcga
     1021 actggttgtc ggtgtcaaac aagacgttcc caattttggt tacaaagatc ccaagaccgg
     1081 tacttattct ggtatcgaaa ccgacttggc caagatggta gctgatgaac tcaaggtcaa
     1141 gattcgctat gtgccggtta cagcacaaac ccgcggcccc cttctagaca atgaacaggt
     1201 cgatatggat atcgcgacct ttaccatcac ggacgaacgc aaaaaactct acaactttac
     1261 cagtccctac tacacagacg cttctggatt tttggtcaat aaatctgcca aaatcaaaaa
     1321 gattgaggac ctaaacggca aaaccatcgg agtcgcccaa ggttctatca cccaacgcct
     1381 aattactgaa ctgggtaaaa agaaaggtct gaagtttaaa ttcgtcgaac ttggttccta
     1441 cccagaattg attacttccc ttcacgctca tcgtatcgat gccttttccg ttgaccgctc
     1501 tattctgtct ggctacacta gtaaacgcac agaactacta gatgatagtt tcaagccatc
     1561 tgactacggt attgttacca agaaatcaaa tacagagctc aacgactatc ttgataactt
     1621 ggttactaaa tggagcaagg atggtagttt gcagaaactt tatgaccgtt acaagctcaa
     1681 accatctagc catactgcag attaaggagg acaccccatg atagatttat catcttggac
     1741 agcctatttt caggattttg gacaattttt caatggtttc ctctttactc ttgccctagc
     1801 gattggatcc tttatcctcg ccatggtctt aggaatcttc tttggtgcca tgtcaaccag
     1861 taaacgtcca attttacgca ttttagctca catctttgtg gaattttacc aaaacactcc
     1921 cctcttggtg cagtttgtta ttgtttttta tggcctacct cttatcagtg accatactat
     1981 catgattcca atttattgga cagctgttct ctgcatggga ctctatcacg gcgcttatat
     2041 cgctgaggtt attcgttcag ggattcagtc tattcctagt ggtcagatgg aggccgcctt
     2101 gtcgcaaggt tttacctata tcagtgccat gcgcttgatt atcttgcctc agaccttccg
     2161 catcattctc cctccattga ccaaccaaat tgttaacctc atcaagaaca cctctactgt
     2221 agctatcatc tctggagtag acttgatgtt tgtgactaag tcttggtcgg ctctcaacgg
     2281 aaactatatc ccagcctttt taggtgctgc tcttctctac tttgctctat gcttccctgt
     2341 tgcccagttt ggtcgcaaga tggagcaagc caacaaaaaa gcctattcac tttaggaggt
     2401 tactatggaa tctattttag aagttttaac cccagataac ctagtcttta tctttaaagg
     2461 atttggcttg accctctata tttctctgat tgccatcatc ctctctacta tcatcggtac
     2521 ggtgctagct gtcatgagaa atggcaaaaa tcctgtctta cgcattattt ccagtattta
     2581 tatcgagttt gtgcgcaacg ttcccaacct tctctggatt tttactatct ttttggtgtt
     2641 caaaatgaaa tccacaccag caggtattac agcctttact ctctttacat cagcagcctt
     2701 ggctgagatt attcgaggcg gtctcaatac cgtagacaag ggacagtacg aagcaggaat
     2761 gtcacaaggc ttcacctcag cccaaatcct ctactacatc attctcccac aagccatccg
     2821 caaaatgcta ccagccatca tttctcagtt tgttaccgtg attaaggata ccagtctcct
     2881 ctactctgtt atcgccctac aagaactctt tggagccagc caaattctca tgggccgtta
     2941 tttcgaacca gagcaggtct tcagtctttg catcctgatt gccctcatct acttcagctt
     3001 taacctagca atttctagcc tgtctcatat gctagccaaa cgttggcaac aagctgc
//
LOCUS       DAVBHS010000178         2996 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVBHS010000178 DAVBHS010000000
VERSION     DAVBHS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.178"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            196..480
                     /gene="groES"
                     /locus_tag="VQ651_001930"
     CDS             196..480
                     /gene="groES"
                     /locus_tag="VQ651_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET2754877.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            496..2118
                     /gene="groL"
                     /locus_tag="VQ651_001931"
     CDS             496..2118
                     /gene="groL"
                     /locus_tag="VQ651_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET2754878.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            2256..2996
                     /locus_tag="VQ651_001932"
                     /pseudo
     CDS             2256..2996
                     /locus_tag="VQ651_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567576.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
BASE COUNT          942 a          578 c          686 g          790 t
ORIGIN      
        1 ctagcttcct agtttgctct ttgattttca ttgagtataa acattaaaaa gtctgagttg
       61 gtctcaggct ttttatcttg agaaagtcag acttttttct tgattatttc tgactaagtg
      121 atacaataga actatgaatt agcactcagg tataaagagc gctaataata tctatctcat
      181 tatggaggaa atcagatgtt gaaaccatta ggggaccgtg tggtcttaaa aatagaagaa
      241 aaagaacaaa ccgttggggg ctttgttctc gcaggttcag cccaagaaaa aaccaaaaca
      301 gcccaagttg tggctactgg acaaggtgtt cgtaccttga acggtgactt ggttgctcca
      361 agtgttaaaa ctggagaccg tgtcttagtt gaagcccacg caggtcttga tgtcaaagat
      421 ggcgatgaaa agtacatcat cgtaggcgaa gctaacatct tggcaatcat tgaagaatag
      481 aaggagaaag taagtatgtc aaaagaaatt aaattttcat cagatgcccg ttcagctatg
      541 gtccgtggtg tcgatatcct tgcagacact gttaaagtaa ccttgggacc aaaaggtcgc
      601 aatgtcgttc ttgaaaaatc atttggctca ccattgatta ccaatgacgg tgtgactatt
      661 gccaaagaaa ttgaattaga agaccatttt gaaaatatgg gtgccaaatt ggtatcagaa
      721 gtggcttcaa aaaccaatga tatcgcaggt gatggaacta caactgcaac tgttttgacc
      781 caagcaatcg tccgtgaagg aatcaaaaac gtcacagcag gtgcaaatcc aatcggtatt
      841 cgtcgtggga ttgaaacagc agttgccgca gcagttgaag ctttgaaaaa caacgccatc
      901 cctgttgcca ataaagaagc tatcgctcaa gttgcagccg tatcttctcg ttctgaaaaa
      961 gttggtgagt acatctctga agcaatggaa aaagttggca aagacggtgt catcaccatc
     1021 gaagagtcac gtggtatgga aacagagctt gaagtcgtag aaggaatgca gtttgaccgt
     1081 ggttaccttt cacagtacat ggtgactgat agcgaaaaaa tggtggctga ccttgaaaat
     1141 ccgtacattt tgattacaga caagaaaatt tccaatatcc aagaaatctt gccacttttg
     1201 gaaagcattc tccaaagcaa tcgtccactc ttgattattg cggatgatgt ggatggcgag
     1261 gctcttccaa ctcttgtttt gaacaagatt cgtggaacct tcaacgtagt agcagtcaag
     1321 gcacctggtt ttggtgaccg tcgcaaagcc atgcttgaag atatcgccat cttaacaggc
     1381 ggaacagtta tcacagaaga ccttggtctt gagttgaaag atgcgacaat tgaagctctt
     1441 ggtcaagcag cgagagtgac cgtggacaaa gatagcacgg ttattgtaga aggtgcagga
     1501 aatcctgaag cgatttctca ccgtgttgcg gttatcaagt ctcaaatcga aactacaact
     1561 tctgaatttg accgtgaaaa attgcaagaa cgcttggcca aattgtcagg tggtgtagcg
     1621 gttattaagg tcggagccgc aactgaaact gagttgaaag aaatgaaact ccgcattgaa
     1681 gatgccctca acgctactcg tgcagctgtt gaagaaggta ttgttgcagg tggtggaaca
     1741 gctcttgcca atgtgattcc agctgttgct accttggaat tgacaggaga tgaagcaaca
     1801 ggacgtaata ttgttctccg tgctttggaa gaacccgttc gtcaaattgc tcacaatgca
     1861 ggatttgaag gatctatcgt tatcgatcgt ttgaaaaatg ctgagcttgg tataggattc
     1921 aacgcagcaa ctggcgagtg ggttaacatg attgatcaag gtatcattga tccagttaaa
     1981 gtgagtcgtt cagccctaca aaatgcagca tctgtagcca gcttgatttt gacaacagaa
     2041 gcagtcgtag ccaataaacc agaaccagta gccccagctc cagcaatgga tccaagtatg
     2101 atgggcggga tgatgtaagc tttctataga aaacaactta taaaaaacac aaaaggaggg
     2161 aatgactaac ccttcttttt ataatattca cttctaaatt gatttgagct cttctaactt
     2221 atatgataaa ataagactag aaaaaggaga agaacatgat cgatgtagaa gaaattctgt
     2281 gcaagatgcc ccccaatcag aagattaatt atgaccgtgt catgcagaaa atggtacaag
     2341 catgggagaa aaatgagcag cggccaacca ttctcgtgca tgtttgctgt gccccttgta
     2401 gtacctatac actagaatat ttgaccaagt atgcagatgt gaccatctat tttgccaatt
     2461 ctaatatcca tcccaaagta gaataccata agcgggtcta tgtcatcaag aaatttgtta
     2521 gtgattttaa tgatcggaca ggaaatacgg ttcagtacct agaagctccc tacgaaccca
     2581 attaataccg aaaactagtt aggggactag aggaggagcc cgaaggtggc gaccgttgca
     2641 aggtttgttt tgactaccga ctggataaaa cagcgcaagt ggctatggac ttgggctttg
     2701 actactttgg ttcagccttg accatcagtc ctcataagaa ttctcaaacc atcaatagca
     2761 tcggaatcga tgtgcaaaaa atttacacga cccactatct tcccaacgat ttcaagaaaa
     2821 atcaaggcta caaacgttca gtagagatgt gtgaggagta tgatatctat cgtcaatgtt
     2881 attgtggctg cgtctatggc agcccaagcc cagaatattg acctggttca agttaagaag
     2941 gacgccacag catgcttgtt ggataaggat gttgagaaag actattctca tatcaa
//
LOCUS       DAVBHS010000179         2942 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVBHS010000179 DAVBHS010000000
VERSION     DAVBHS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.179"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            93..722
                     /locus_tag="VQ651_001933"
     CDS             93..722
                     /locus_tag="VQ651_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET2754879.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(845..2737)
                     /gene="pepO"
                     /locus_tag="VQ651_001934"
     CDS             complement(845..2737)
                     /gene="pepO"
                     /locus_tag="VQ651_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HET2754880.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT          785 a          688 c          587 g          882 t
ORIGIN      
        1 tcaaagcact gctttgaggt tgcagataaa gttgacgcgg tttgaagaga ttttcgaaga
       61 gtattagtac attctttgag attggagtta gtatgaaaat ccataaaacc gtgaatcctg
      121 ttgcctatga aaatacctat tatctagaag gcgaaaagca cctcatcgtc gtcgatcctg
      181 gtagtcattg ggaagccatt cgtcagacaa tcgagaagat caacaaaccg atctgtgcta
      241 ttctcttgac ccacgcccat tatgaccata tcatgagtct ggacttggtt cgcgagacgt
      301 ttggcaatcc tcctgtctat atcgcagaga gcgaagccag ctggctctac actcctgtcg
      361 ataatctctc cggtctccct cgccacgatg atatggcaga tgtggtcaca aaacctgcag
      421 aacacacctt tgtctttcac gaagaatacc aactagagga atttcgtttt aaggttctac
      481 cgaccccagg gcactctatc ggtggtgttt ccctagtctt tcctgatgct cacctagtct
      541 tgacgggaga tgctctattc cgcgaaacta tcggacggac cgaccttccg actggtagca
      601 tggagcaact ccttcatagt atccagaccc aactcttcac cctaccaaac tacgatgtct
      661 atccaggaca tggtccagct actactatcg ctcacgaaaa ggccttcaat ccctttttct
      721 agcaagatga tgacaatcga aatttaagta aactatccag caaatctttc tattacaaaa
      781 ggcatcctat caaggttttc acacgtgatt ggatgccttt tttctgatga ctagattttt
      841 tgtattacca aataatcacg cgctcctctg gtgaacgcca cattccgtct ccttctttga
      901 catcataggt tgtaaagaaa tcgtcgaagt ttggtacttg cacattgaca cggagtttgg
      961 ctggtgcgtg cacatcgacg ctagccaaaa gtttcataaa ttctggtcga cctttcatgc
     1021 gccagatgcg accgaagttg tagaagaact cttctgctga gaagtctgct tctctcttag
     1081 ctgcttcaag cgctgctgcg attcctccca agtcagccac gttttctgat acagtcaatt
     1141 taccgttaat ggttgctcca taagaatcct gtccatcaaa ttggtcaatg actttttgtg
     1201 ttttctcctt gaaggcagca tagtcgctct ctgtccacca atccttgagg ctaccatttt
     1261 cgtcaaagga agccccgtta gtatcaaagg cgtgggaaat ttcatgggca atcactgccc
     1321 caataccacc gtagttagca gaagatgact gatgcaagtc atagaaaggc gcctgtaaaa
     1381 tggccgctgg aaagacaatc aggttcttct gaggattgta gtaggcattg accatatgag
     1441 caggcatgcc ccattcctta tagtctacag gctggttcca cttactccaa ctgtgcttga
     1501 tttccacacg cgcaaaggct agagcattct caaaaagact ggcagtttca ttcactacct
     1561 tatccttgta acgtgcaggc aattcttctg gatagccaat ataaggtttg atcacattga
     1621 gcttcacgat agcctgttta caggtttctg gagtgagcca gtcattctta agcagacgct
     1681 ccttatagac atcaatcatg gttgccactt ttttctccac atccgccttg gcttctggag
     1741 agaacttctc acgggcgtac caaagcccca gggcttgctt gaaaggttcc tgtgctagat
     1801 gataagctgc tttgacctta tcttttgcct ctggaactcc agaaagggca cggctgtagg
     1861 caccagacaa aacacggata tcctctgtta aatagctggt tgaaagattg acaacactca
     1921 aaatcaaggt tgctttaagg agagaccagg cttcctcact gtaaaattgc tctgctgctt
     1981 gccagaaacg ttcctcgtct acaataacct tgtctggtaa ttgcccaata actgctttga
     2041 agaagtcatc caaaggtagg gcaggcgcga atttcttgaa atcttcgtaa gaatatggat
     2101 gatagagttt agcatattct gaactttctt cattagagag caccactgcc gcaactcggc
     2161 ggtccaattc aagtcttttt tctagcaagt cttcaatttc ttcatcagag aaatcataag
     2221 ccttgaggag atttgcgctg ctttctttcc aaagagtcaa gagctcttcg cgctgaggat
     2281 gttcttctgc atagtaggtc gtatctggca agattgtgct tggagcgcta gcccatagaa
     2341 cattgattct agcatccata aagtctggcg atacaccaaa aggaaggaag tttggttttc
     2401 ctgcaagctc aaactctgct agtttagctg taaaatccgc aaaagtttcc aattcttgga
     2461 attctttaag gagtggtaag acaggtgtga taccgtcagc ttctctcttg tcaaaatcac
     2521 gaactaggcg gtggtatttg acaaagtttt ccaagatagc atcctcaggc acttcttcac
     2581 ctgctaacca cttgtctgtt gtcgccagca tcaggtcttc aatttcctgg tctaaatcaa
     2641 caaaacctcc tgtttgagac ttatctgctg ggatttcagc tgtctgttgc cattctccat
     2701 tgatagcatc ataaaaatca tcttgataac gtgtcatctt gttctcgctt tcatttgtat
     2761 ttgcatttat cttaacaaaa atcactaggg aatgcaatta ataaccattt aactattatt
     2821 ttttagctat tataaaaaac ctttccatga taaaacgcat aattccaagt tttttacact
     2881 tgatactatg cgttttataa tttagaaatt tatcctgaaa gttatcttta gaatctattt
     2941 tt
//
LOCUS       DAVBHS010000180         2891 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVBHS010000180 DAVBHS010000000
VERSION     DAVBHS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.180"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(91..1140)
                     /locus_tag="VQ651_001935"
     CDS             complement(91..1140)
                     /locus_tag="VQ651_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2754881.1"
                     /translation="MKDLFLKRKQAFRKECLCYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEAIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(1137..1868)
                     /locus_tag="VQ651_001936"
     CDS             complement(1137..1868)
                     /locus_tag="VQ651_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2754882.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            1936..2346
                     /locus_tag="VQ651_001937"
     CDS             1936..2346
                     /locus_tag="VQ651_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET2754883.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMNATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            2356..2643
                     /locus_tag="VQ651_001938"
     CDS             2356..2643
                     /locus_tag="VQ651_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754884.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
BASE COUNT          967 a          649 c          549 g          726 t
ORIGIN      
        1 tctctacgga ccaaccatta tcctgtagta acaaaaaaga agctgagaca gactgaactc
       61 aacttctttt ttagtgtcgt atcagcaatg ttagtcctgc atctgacgtt ttacctgata
      121 aggcaaatac aagactagta aaaccaaacc agctcctagt aaggctagaa gggccagttt
      181 ttcttggaag gtaatcaacc caacaactaa ttccacaagt aaaacaaaac tggtcaatcc
      241 tcgaactacc tcctgtaagc ctttttcctt ttgccccttg tccagcggaa agagttgggt
      301 caaatactgg tagtcaaagg catgatagag ggccagcaac tggaagagca agaggtagtt
      361 aaagagaact accactgctg tcgcaatcca agcttgctcg ataaaaacct gcgccagtac
      421 ggaaagtaat agaagacgaa gactgagagc aaagaggtcg ccatttcgca gataagaacg
      481 cagatagaga ttttgccaaa tcttcccagg caccttctga acagccttta agataaagtc
      541 cagataggca cgacgcttaa cgctgtttga aattcccttg acctgcgtaa agagggcaaa
      601 gaaacgaagc aagacttgct tacgcttgct ttcttgagaa ataacatagt cccagtccag
      661 tccagtttca gtaaaaaatt tgctggcctt ttgacgaaag tggaaatatt ttcctacccc
      721 caataaaagc acatatagca gaaaaactgg caagccataa cccattgcta aaaataaagg
      781 cgcaaataac agcaagaaaa gggtctgtac aaagagccaa aagactaggg aaatgccagt
      841 ttgacgcttg agatggagct tgattgcctc ttctccaact aagagaaaga gcttgtctgg
      901 agcctccata taggtggcaa ttcctcccca aagtaagagt aaaacagacg taattcctac
      961 aaacaaaagg ataggccaat gattttcagg aaaatgttgt aagagttgac tgtactggta
     1021 ggctagaaag cccaacagga caagcaggaa caagacaaag tggtcattga gcacatagcg
     1081 cagataacaa agacactcct tacgaaaggc ctgctttctc tttaaaaaca agtctttcat
     1141 agatcctcct ctttggtcag agccaagtaa atatcattca aactagcctc aggcatatca
     1201 aaggcttcac gtagttgcag gagattgcct ttggcacgca cctctccctt gtgaagaatg
     1261 acaaaggcat cacacatctt ttccgccgaa tccagcacgt gggtactcat gagaatagac
     1321 ttgccctttt gcttctccac ttccaaaagc tgaatcaaat cagaaatagc cagcggatca
     1381 agaccaagga aaggctcatc cacgatgaaa agacttggat ccaccacaaa agcacagata
     1441 atcatgacct tctgcttcat cccttttgaa aaatgaacag ggaaccagtc taatttctgt
     1501 tccaaacgga acatttttaa caagggctct actcgttcga aagccacttt ttgctcaata
     1561 ccgtaagcca tagcaaccgt ttcgatatgc tctctgaggg tcaattcctc atacagacta
     1621 ggcgtctcag gaatgtagcc aatctgcttg cggtagctag tcgcatctcc ttgcagagtc
     1681 aggccattga tattgatgga gccactataa ggtgccaaca gaccgataat ctcattgatc
     1741 gtcgttgatt tcccagcacc attgagacca atcaaaccga ccaactgccc actttcaaca
     1801 gtaaaggaca catctttcaa aacaggaaca tgaacatagc cacctgtcag gtttttaatt
     1861 tctaacatat tttctccaaa tctggtataa tgtagctata ttatatcaaa attcagtaca
     1921 gtagaggtag attttatgtc agattgcatt ttttgtaaaa tcatcgcagg ggaaattcct
     1981 gcttcaaaag tatatgaaga tgagcaggtc cttgcctttc ttgatatctc tcaagtaaca
     2041 ctaggacaca ccttggtcgt gccaaaagaa cactatcgca atcttttgga gatgaatgct
     2101 acgagcgcca gccaactctt tgcccaagta ccaaaagtag ctcaaaaagt catgaaagtc
     2161 actaaggctg ctggtatgaa tatcatttcc aactgtgaag aagtcgctgg tcaaacagtt
     2221 tttcatactc acgttcacct tgtgcctcgc tacagtgctg acgatgacct caagattgat
     2281 tttatcgccc acgaaccaga ctttgacaaa cttgctcaag tcgctgaaac catcaaaaac
     2341 gcttaaggag ttgctatgaa attatccaac ctactgctat ttgcaggagc tgcagccgga
     2401 agttatctgg ttacaaaaaa tcgccaaacc atcacagatg aagtcttgaa taccactgac
     2461 cgcgttcaag ctatcaaaga cgatgtggat attatccaaa atagcctgca aatcatcgac
     2521 cagcaaaaag aacttatcaa ggaataccaa gaagacttga cttacaagtt taaggtcttg
     2581 gaaaaggata tccagactag actagctgtg ataaaagaaa tgcagggaac tgaagataag
     2641 taaaaagagc cccccggctc ttttttaaaa cgtaaacaag ttatagtgaa ttgaatttag
     2701 aatagcacat catagtttct aaagcattgt tagagattac tttaaaatcc tcttatcaat
     2761 ttgttcatat tctatttcaa tctactatat atatcccatc aactatgcat cataatttag
     2821 gtaactcata ctcaataaaa atcaaaaagc aaactagaaa gctaggcaca gactgctcaa
     2881 aacaccgttt t
//
LOCUS       DAVBHS010000181         2891 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVBHS010000181 DAVBHS010000000
VERSION     DAVBHS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.181"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(481..1206)
                     /locus_tag="VQ651_001939"
     CDS             complement(481..1206)
                     /locus_tag="VQ651_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754885.1"
                     /translation="MKLLKNELIKSKIFLFIIVDICIQILVILAIKTYILDISAPYSE
                     LDYNKYWYVLHALIYMPMIFPIQILYQNLREALIEDNNNGWNIMVINTNNLVKIIYIK
                     VTINIVRCFICYFVYTIFSLIQLGGMGTDMLLTNIIFPNIIFPNIMSFLLFLPIAIFM
                     QICCIRFDSILAKALPNILLILIVLITFQSDWNIFIPATYYYTEIQSTTNLGIKLLVC
                     IWIMGFEFSLLPKLIKLKEQNLV"
     gene            complement(1219..1956)
                     /locus_tag="VQ651_001940"
     CDS             complement(1219..1956)
                     /locus_tag="VQ651_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2754886.1"
                     /translation="MIKLEFLKQKKSILWFVLIFPIILNALLYIDLTFRYRGYLLVHQ
                     NELALSNWQLIFKEQTIFYFSELFYLVLSLIIYEVFAVEFKNDAWLTVISLPFRNKYT
                     INSKLLITVVYTFTFWLSDYISLYVIGKAIDNSLEIGLIFFLKTFTIQLISSLMIMLL
                     YFLTLVLIRKISGIIPIGIIMMILTISIYYNDYNFKIYLPFTYLSHAFRVTESQFYMI
                     LLSNIIIIVLFYILIRKLNERSFEMKL"
     gene            complement(1949..2677)
                     /locus_tag="VQ651_001941"
     CDS             complement(1949..2677)
                     /locus_tag="VQ651_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2754887.1"
                     /translation="MAIVEIINLTKSFKDIEVIHNTSFYLNKGKVYGFVGPNGAGKTT
                     IIKMILGILKPDSGKITIFNQTVEQNSKNILSRIGLVLGPSFYGHLDAYKNLKLIANM
                     KGLSLDTERLNEYLSMVGLKDVKKKKVKNFSMGMKQRLSIAASLLGSPEILIWDEPIN
                     GLDPQGVIEIRSLIRFLQEKKGITFLISSHILSELDKVISDIIIINYGKVEFFGSCHY
                     LLQKYNCRNLEEAYLACLAGGEYD"
     gene            complement(2711..2881)
                     /locus_tag="VQ651_001942"
     CDS             complement(2711..2881)
                     /locus_tag="VQ651_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754888.1"
                     /translation="MKKVNEYVISTAASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
BASE COUNT         1095 a          442 c          343 g         1011 t
ORIGIN      
        1 tggacatttt gaactaaaat taatttatta aacatatctc ataagcatat ctattatagg
       61 tgagacaata ttaagaaaga cggttaaaaa acaacctgac tccactggaa ttcaggaatc
      121 aggtcgctta agattttata ttattttact gtctacttga cagggagctg ttcaccaatg
      181 ggaatctata gctttttttg tgactgagaa ctattttatc tcagacttct tcaacacacc
      241 ttattattgg gcacttacgg aaggttaatg aattatttaa aataaaagta aagagaagca
      301 aacagacact ctatagctgt tagacgcttt ttagagtaat aacaatttga taaagtgaat
      361 acaacttgct taattaagcg taagaaacca gaatcataaa atcaattact ttgaagtcat
      421 gactcacgtt gtattgtttc attgttaatc gtattcaatt atttaatttt cgagcaacat
      481 ttaaactaga ttttgttctt tcaattttat tagttttgga agaagggaaa attcaaatcc
      541 cataatccag atacaaacta ataatttaat acctaagttt gtagttgact gaatttctgt
      601 ataataataa gttgcaggta taaatatatt ccaatcactc tgaaacgtga ttaaaacaat
      661 aagtatcagc aaaatattag gtaaggcctt tgctaaaatg gaatcaaatc ttatacagca
      721 aatttgcata aagatagcta taggtaaaaa taacagaaat gacattatat tgggaaatat
      781 tatattggga aatattatat tggttaataa catatcagtt cccataccac ctaattgaat
      841 taaagaaaaa attgtataaa caaaataaca aataaaacat ctcacgatat ttatcgttac
      901 ttttatatag atgattttca cgagattatt agtgttaatc accatgatat tccaaccatt
      961 attattatct tcaatcaatg cctctcttag attttgatac aaaatttgta taggaaaaat
     1021 cattggcata tatattaaag cgtgtaaaac ataccaatac ttattataat caagctctga
     1081 gtatggtgcc gaaatatcta aaatatatgt ttttatagct agaatgacta gaatctgaat
     1141 acagatgtca acaataataa ataaaaatat tttactctta atcaattcat ttttaagtaa
     1201 cttcattttt attacctatt aaagtttcat ctcaaaactt ctttcgttca atttacgaat
     1261 taatatataa aaaagtacga tgattattat attagatagt aaaatcatat aaaattgact
     1321 ttcagtcact ctaaaagcat gagaaagata agtaaatggt aagtaaatct taaaattata
     1381 atcgttgtag tatatgctaa ttgttaaaat catcattata ataccaattg gtattattcc
     1441 agaaattttc cttataagta ctaaagttag aaaatataat aacattatca tcaacgagga
     1501 aattaattga atggtaaatg tttttaaaaa gaaaatcaaa ccaatctcta aactgttatc
     1561 aatagccttc ccaataacgt ataagctaat ataatcagac aaccaaaaag taaatgtgta
     1621 aaccactgta atgagtaatt tagaatttat tgtgtacttg tttctaaatg gcaatgaaat
     1681 tacggttaac catgcatcat ttttaaattc tactgcaaac acttcgtaaa ttattaaaga
     1741 tagaactaaa taaaatagtt cagaaaaata aaatattgtt tgttctttaa aaattaactg
     1801 ccaattagat aaagcaagtt cattttgatg caccaataaa taacctcgat acctaaaagt
     1861 taagtctata tataacaaag cattcagaat tattggaaaa ataagaacaa accataagat
     1921 gctttttttc tgctttagaa actctaattt aatcatattc acctccagct aaacaagcta
     1981 agtaggcttc ttccagattt cgacaattat acttttgtaa aaggtagtgg caagaaccaa
     2041 aaaattcaac tttcccataa tttataatta taatatcaga tatcacttta tccagttcac
     2101 taagaatatg acttgatatt aaaaatgtaa tacctttttt ctcctgtaga aaacggatta
     2161 aacttctaat ttcaattaca ccctgtggat caagtccatt gattggttca tcccaaatta
     2221 atatttctgg actacctaac aatgatgcag ctatacttag acgttgtttc atgcccattg
     2281 aaaaattttt cacttttttc tttttaacat cttttaatcc taccattgat agatattcat
     2341 taagtctttc agtatctaaa ctaagccctt tcatatttgc tattaacttc aaatttttat
     2401 aagcatcaag atgaccataa aatgaaggtc ccaaaactaa tcctatccta gataatatat
     2461 ttttggaatt ctgttctacg gtttgattaa aaatagttat tttcccagaa tctggtttta
     2521 aaatccctag aatcattttg ataattgtag tttttccagc cccatttggt ccaacaaaac
     2581 catatacttt tcccttattt aaataaaatg aagtgttatg aataacttca atatctttaa
     2641 agctttttgt tagatttata atttcaacta tagccatctc tactccttag tttacagcaa
     2701 aaaattatgc ttaattctta ttgtttttgt aaacaatcag cgaacccaat actattccat
     2761 tcaacaaacc taaagaaata ccatattcaa ctggaaaatc taaaaaaatt gcaaatacta
     2821 ttccaatcat cacccctagt gaggctgctg ttgaaattac atattcattt actttcttca
     2881 ttttctttac a
//
LOCUS       DAVBHS010000182         2865 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVBHS010000182 DAVBHS010000000
VERSION     DAVBHS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.182"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(201..494)
                     /gene="rpmA"
                     /locus_tag="VQ651_001943"
     CDS             complement(201..494)
                     /gene="rpmA"
                     /locus_tag="VQ651_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET2754889.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(511..855)
                     /locus_tag="VQ651_001944"
     CDS             complement(511..855)
                     /locus_tag="VQ651_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HET2754890.1"
                     /translation="MIQAVFERAEDGELRGAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(871..1185)
                     /gene="rplU"
                     /locus_tag="VQ651_001945"
     CDS             complement(871..1185)
                     /gene="rplU"
                     /locus_tag="VQ651_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET2754891.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYIEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(1221..1308)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(1403..2002)
                     /locus_tag="VQ651_001946"
     CDS             complement(1403..2002)
                     /locus_tag="VQ651_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HET2754892.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQAGNVVFLSVELIQNNVSDVR
                     DKLLTLITFIMGVFLLTIYKEKLRIVKKPILSLIPLAILSIIIGFVPQTVDNIYLVQP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGDVFSAYLTIFYHEKTILGVPIMMSIFYLSMLFA"
     gene            2472..2771
                     /locus_tag="VQ651_001947"
     CDS             2472..2771
                     /locus_tag="VQ651_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET2754893.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          930 a          585 c          481 g          869 t
ORIGIN      
        1 acttgagtac ggcaaggtga aactgacgtg gtttgaattt gattttcgaa gagtatcaat
       61 ccactataca tattaaaaag tatggatatc aaaacaaaaa aacaggctct ccgaaaactc
      121 ggaaagcctt attttaagct gtttctagct tcctcacctt tacatttcaa ggttcgggat
      181 aaaaggttca atggaccttt ttatttagcg attgggtaaa cagaaacttg ttttttatcg
      241 cgtcctttac gttcaaagcg tactacgcct tcaactttag cgaacaaagt atcgtctcca
      301 ccacgaccaa cgtttacacc tggatagatg tgtgtaccac gttgacggta aaggattgat
      361 ccacctgtta cagtttgtcc gtcagctgct ttagctccaa gacgttttgc ttgtgaatca
      421 cgtccgtttg atgtagaacc tccacctttt ttgtgggcga aaagttgcaa gttgttaaga
      481 gtcattttta acataatgtt ttcctccgtg ttagttttct gtgataactc tggtttggac
      541 gaactcagaa tagttctccg ataagtttgc catacctaag aaaaatgatt caaagaataa
      601 ctgggtcatt tctctctggt gtgaaggaag atcttttggt atttcaacca tcagatagcc
      661 accttcatct tcgtttaatt ctaggattgg ttcatagcct gcaaatttct caatagaatt
      721 gataaagtta atggcaagcg tagaaaccga tgcacacacg acatctaagc cgtattcgcc
      781 actctcggcg tgtccagtaa tttccgcacc cctcagctcg ccatcttcgg ctctctcaaa
      841 gactgcttgt atcatgtgtt ctccttaaaa ttaagcgtta attgcgttga tgacaacttt
      901 tgtatatggt tgacggtgac cttgtttacg gtggctacct tttttaggtt tgtacttgta
      961 agtaacaact ttcttttgtt ttccttgttt ttcaacagtt ccaactacag tagctccagc
     1021 aacaagtgga gttccgacaa cagtgttttc accaccaaca agaacaactt cgttaaaagt
     1081 aacttcttga ccagcttcaa cgttcaattt ttcaatgtaa actgcttgac caacttcaac
     1141 tttaacttgt tttccgccag ttttgataat tgcgtatgtg ctcattatgc acctcctatg
     1201 atttttatgg gtttccccga ttttttgtga agactcgcct agcatcgtgg gacaaaccac
     1261 ttaaacttga ataatcaagc aacgatgttc gtgcggttgc acaggatcgt gcatagtcaa
     1321 ctcttcaagt atagcatatc tcctattttc ttacaagtaa taacacctaa aatcaagctt
     1381 tttcttttac ttttttctgc cactaggcaa aaagcatact gagataaaaa atgctcatca
     1441 taataggaac accaagaatg gtcttttcat gatagaaaat cgtcaaatag gctgaaaaga
     1501 catcgccaag gacaaaacta ctaagcaggc taacaaatat gaacccttca cgcaaaaaag
     1561 gagtgtgctt agttcggaaa taatctccaa aagctagcat tgttcgtttg atattccctg
     1621 tcataaaggc attattgtag gcaatacccg aaacctcacc aaaagcagtt gtcaccagtc
     1681 ccatacagaa ggccaagggc tgcactagat agatattatc tacagtttgt ggcacaaaac
     1741 caataattat tgataggatt gctaaaggaa tcaaggacag aataggtttt ttcacaattc
     1801 tcaatttttc cttataaatc gttaataaaa agactcccat tataaacgtt attaaggtta
     1861 aaagcttatc cctaacatcc gaaacattat tttgtattag ctctaccgaa agaaagacaa
     1921 catttccagc ttgtccagct acaagggtat tccctcgaac aataaaagtg taagcatcca
     1981 catatccagc acaaaatgtc aaaaaagtgc taacctttta gactgacgtg atatttttct
     2041 tataggtaat aacctcattt tccctcccat tgtattttct cttagaaata ttgtaccatt
     2101 tctttctaaa aaatcgtaga ctaccattta gattttacta ttagcataaa aataataata
     2161 gacaactatt tatccaaaaa tagatagagg taacatgttt gcaaacaaag catacgaacc
     2221 tttagtaaaa tcatttccat gaaactagaa tagagcccct cttagcaaaa atcattattt
     2281 taatttattt ctaatcactc cttgacataa ataactctca ccaataaaag actatgtctt
     2341 aaaaaaatgg tataataaaa tcaatacctg ggcttgatgg ctatgctact aataacaatt
     2401 aggagagaaa atcaggcact tgttaacaac aaggattatc cccttgagat gaaaggaact
     2461 ttagaaatct tatgatgaac atgcaaaaca tgatgcgtca agcacaaaaa cttcaaaaac
     2521 aaatggaaca aagccaagct gaacttgctg ctatgcaatt tgttggcaaa tctgctcaag
     2581 atcttgtcca agcgacctta actggcgata agaaagttgt cagcattgat ttcaatccag
     2641 ctgtcgttga cccagaggac cttgagactc tttctgatat gaccgttcaa gccatcaact
     2701 ctgctcttga acaaatcgat gaaactacta agaaaaaact gggtgctttc gctgggaaat
     2761 tgccattcta aaaacaagga gctagaacaa tacttgtcga taacaaaggc taagaaaggt
     2821 gcaaaaatta taaaaaacgc atagtatcaa gcaacctaaa acttt
//
LOCUS       DAVBHS010000183         2807 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVBHS010000183 DAVBHS010000000
VERSION     DAVBHS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.183"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(91..1332)
                     /locus_tag="VQ651_001948"
     CDS             complement(91..1332)
                     /locus_tag="VQ651_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET2754894.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITAYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFIDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            1435..2544
                     /locus_tag="VQ651_001949"
     CDS             1435..2544
                     /locus_tag="VQ651_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET2754895.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT          831 a          470 c          532 g          974 t
ORIGIN      
        1 ttctttgatt ttgagtatta gatttactca aaatcaagga ttttgaagat gaacttgtat
       61 caaaaaatcg cggtttaaaa tcgcgatttt ttataatttc tcgttaacaa agcggacaaa
      121 ctgattccac caaactttta agaagaaggc tttttcaatt ttcttgtctg ctaccatttc
      181 gaaactaggg cgctctgtgg tgatgtaacc ttgaccaatc aagtccttgt cttcataagt
      241 caaatggcca accactgttc cagcttcaag tggtgctggg attgctttgg aatcaggtgt
      301 gaattgaaca gattgggaag attgattccc aacacgttcg attagataga tatcctctgg
      361 agccactgca gttactgtat cttcttttcc atcttgtaca ggggctttgc tatcttgata
      421 ggcatcgcct tgttgaacga ttttgcgaag tgtaaatgta gaagaaatat aatccattag
      481 ggaagatgta gctgtaaatc gagcgtaagg attattgtct tgatgatctg catttaaaac
      541 aactgtgatg actctcatgc ctttttcgac agtagtacca acaaaagact ctccagcctt
      601 atctgttgtt cctgttttta gcccatcaaa accaccacgg taagcaggca taccttctaa
      661 catgtagttg gttgaagtga ttgtcatccc agcaaaagta gaagaaggtt ttttggtgat
      721 ttctaagact tgtgggtatt ttttgatgag gttgcgagca acgatagcga catcataagc
      781 actaagctta ttttcctcat cttttttaga acctgggtaa atgttatccc ctagagtttc
      841 attgttaaga cctgtcgtat tgacaacagt ggcatcctga attccccatt ccaagagttt
      901 tgcccgcatc atatcgatga aatctttttc tgagccagca attttctcag ctagggcaat
      961 agcggcgctg ttggcactag ataccagagt tgcttcaagc aactcttcga cagtataatt
     1021 acgggcctcc ataggaatat tactggcttc agaatttgtc gtcaattgat aaggataatc
     1081 agaaatatct acaggagtgg agagggtaat acttccgttt tccaaagctt catagaccag
     1141 ataagcagta atcaattttg ttatggaagc aatttcgaca ggttgcgttg catccttctc
     1201 atagagaatt ttaccagtat ttgcctcaac agcaatcgca tgtttagcgg caatggtaaa
     1261 atcttgagca acagcagtag aagcaccccc taaaagagag acagttaaca aagttaaaaa
     1321 tatttttttc atagtagtct tattctatca taaagaaaaa aaatattctt gctttaataa
     1381 ttcatctgtt aagctttttg aaaatatggt aaaataaagt gagggaggta actcatgttt
     1441 cgtagaaata aattattttt ttggaccaca gaaattttac tcttaaccat catcttttac
     1501 ctatggagac agatggggtc tttgattaac ccttttgtta gcgtgcttaa tacaattatg
     1561 attccatttt tattaggggg ctttctttat tatttgacaa accctattgt tactttctta
     1621 aataaagtct gtaaactcaa tcgtttgctt ggtattttaa ttaccttgtg tactttggtc
     1681 tggggaatgg tcataggtgt tgtctatctc ttacctattt tgattaatca gttatctagt
     1741 ttgattatat ctagtcaaac tatttatagt cgagtacaag acttaatcat agacttatct
     1801 aattatcctg cgctccagaa tttggatgta gaagctacaa ttcagcagtt aaacttatcc
     1861 tatgttgata ttcttcaaaa tatcctaaat agcgtatcaa atagtgtggg gagcgtcttg
     1921 tcagctctta tcagtactgt tttgattttg attatgactc cagttttttt ggtttatttc
     1981 ttattagatg gacataaatt cttgcccatg cttgaaagaa cgattctaaa gagggatcgc
     2041 ttgcatattg caggcttatt aaagaattta aatgcgacga ttgctcgcta tattagtgga
     2101 gtttcgattg acgcaatcat tataggttgt ttggcttata ttggctatag tattattggt
     2161 ttaaaatatg ctttagtttt tgccattttt tctggtgtag ccaatttaat tccttatgtg
     2221 gggccaagta ttggtttgat tcctatgatc atcgcaaata tattcactga tccccataga
     2281 ctgctgattg cagtgattta tatgcttgtt gttcagcagg tagatggcaa tatcttatat
     2341 cctcgaatcg taggaagtgt tatgaaggtt catccaatca cgattttagt tttacttttg
     2401 ttgtcaagca atatctatgg tgtagttgga atgattgtcg cagtgccaac ctattctatc
     2461 ttgaaagaaa tttctaagtt cttatcccgt ttgtatgaaa atcataaaat aatgaaagaa
     2521 cgagaaagag aattagctaa gtaaaagtca ggagaaccct gatttttctt tactggaagt
     2581 ggcctttaga ttagaagact gaaaataagt taaagtctta aactaatttt cacagctaag
     2641 aatagtagaa gttaatctga taaaaatcga aaaaaccagt ggaattctgt gtcagggtaa
     2701 gttccactgg ttttcatagt ctattaaagt tcgaatgaaa cctatttata gtagattgaa
     2761 actagaatag tacacctcta attctaaaac attgttagaa atcgatt
//
LOCUS       DAVBHS010000184         2799 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVBHS010000184 DAVBHS010000000
VERSION     DAVBHS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.184"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..941)
                     /locus_tag="VQ651_001950"
     CDS             complement(<1..941)
                     /locus_tag="VQ651_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET2754896.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(919..2682)
                     /gene="aspS"
                     /locus_tag="VQ651_001951"
     CDS             complement(919..2682)
                     /gene="aspS"
                     /locus_tag="VQ651_001951"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET2754897.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVVNATLGTLRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
BASE COUNT          813 a          662 c          557 g          767 t
ORIGIN      
        1 taattatcta tctcaacaaa tcttcctaga atatgaacat tttccgagac agagacaaag
       61 gagcttggat ccacttgtgt cataatctgt ttaaattcat taaactctgc acgtgtaatg
      121 acagtgatta aaactgcctt tctctcgtga ttataggttc cttctgcatc gtggatcatg
      181 gttgctccgc ggtgcaattt tttatggatt ttttcaatta ccttctctgg atgatttgtc
      241 acaatcatgg cctgcatacg cttttgctta gtaaagactg cgtctgtcac acggctagag
      301 acaaagatgg taatcataga ataaagagcg tatttccaac caaaggtcaa acctgctatc
      361 agcatgatag ttccatttac caagaaagaa atactaccga cattcttacc cgttttctta
      421 cgaatagtca ggctgacgat atccgtccca ccactggaga tattgtttcg aagagcaaaa
      481 ccaatcccca aacccataac aacaccccca aaaagggaat tgataatggg atcctccgtc
      541 aaggttgcca cagggacaaa ctggataaag aaggaactca tagataccgt gataaaggta
      601 aagacggtga acttatggcc aatctgatac caagctaaga ccatcaaagg gaagttaatg
      661 gcgtagaagc ttagcgaaat cggaatatga aaaccaaacc agtgattact caaggcagag
      721 ataatctgtg ccagacctgt tgcaccactc gaatacacat gccctggttg gaaaaagaaa
      781 ttaactgcta ctgctgataa aaaaccatag accagagagg ccgaaatctt ctcatcatac
      841 ttttctcgag agatactttg taagacacgt aaaattttta tctgataagc aaagcggcgc
      901 agataatagc gccaccgctt aattcgtttt gtttgtttca tcttcttcta cttgtaagct
      961 gagttcctct agttgtttga gagcgactgt tgatggagct tgtgtcattg ggtcagttgc
     1021 cttgttgttc ttaggaaagg caatgacttc acggatattt tcttctccag caagcaacat
     1081 gacaaaacgg tcaagcccga tagccaaacc accgtgcggt gggaaaccat agtccatggc
     1141 ttcaagaagg aaaccaaact ggtcattggc ttcttcagtt gagaaaccaa gagccttgaa
     1201 catgcgttct tgaaggtctt tttggttgat acgaaggcta ccaccaccaa gctcataacc
     1261 gttcaagacg atatcgtaag caatggcacg aaccttagcc aaatcacctt ctaattcgtg
     1321 agcagtctct tcctgtggaa gtgtgaatgg atggtgggcg ctcatgtagc ggccttcttc
     1381 ttcagaccat tcaaacatcg gccagtcaac cacccaaagg aagttgaact tatcattatc
     1441 aatcaagcca agctctttag caatacgtcc acgaagggta cccagtgttg cattaaccac
     1501 ttcaagcgta tccgccacaa agagaaccaa gtccttatct tcaagagcaa gcgctgttgt
     1561 caattcttct tggataccag tcaagaactt ggcaactggt ccgtttaatt ctccatcaac
     1621 caccttgacc caagcaagac ctttggcacc atactgtttg gctacttccg tcatcttgtc
     1681 gatgtcttta cgtgaatagt tgtccgcagc tcctttgacc acaatcgctt ttacagcagg
     1741 tgcttctgaa aagactttaa agtctacacc tttgaccact tctgtcaagt cctgaagcaa
     1801 catgtcaaaa cgagtatctg gcttgtcaga accgtaaaga gccatagcat catcgtattt
     1861 catacgaggg aacggtagcg ttacttcgat gccttttgtt tccttcatca cgcgcgcgat
     1921 caagccttct gtaatatctt ggatttcttt ctcagtaagg aaggacgttt ccaagtcgac
     1981 ctgagtaaat tcaggctggc ggtctccacg caagtcctcg tcacggaaac atttaacgat
     2041 ttggtagtaa cggtcaaaac cagcattcat caagagctgt ttcgtgattt gtggactttg
     2101 aggaagagcg taaaaatgcc ccttattaac acgagacggc actaaataat cacgcgcccc
     2161 ttcaggcgtt gacttagaaa ggaatggtgt ctccacgtcg ataaactcca actcatccaa
     2221 atagttgcgg atagagtggg tcaccttggc acgaagttta agattttcca acatttctgg
     2281 acgacgaagg tcaaggtaac ggtaacgcaa acgtgtatcg tcatttgcct caatgccatc
     2341 cttaatctca aatggtgttg tcttagctgt gttaagcaca ataagagctg tcacgtttaa
     2401 ctcaaccgca ccagttggca acttatcatt ggcttgctca cgcgcagcga cctgaccagt
     2461 cacctcaata acaaattcgc tacgaaggct ttcagctgtt gccataacct ctgcagatac
     2521 tttttcaggg ttgataacca actgcatgat tccttcacgg tcacgaagat cgataaagat
     2581 caaaccacca aggtcacgac gacggccaac ccatcctttc aaggttattt cttgtccgat
     2641 gtgttcctca cgaacacgac cagcatacat actacgtttc attatttctc tcctctttta
     2701 ttctgttact attttaccat aaaagcgcag gctcttcatg aaaatcatca gaaaagtttg
     2761 ccagtcttta aaagtcaggt gaaagcccta aaaattagc
//
LOCUS       DAVBHS010000185         2770 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVBHS010000185 DAVBHS010000000
VERSION     DAVBHS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.185"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            264..2588
                     /gene="pflB"
                     /locus_tag="VQ651_001952"
     CDS             264..2588
                     /gene="pflB"
                     /locus_tag="VQ651_001952"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HET2754898.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT          878 a          572 c          543 g          777 t
ORIGIN      
        1 ctgcggctag cttcctagtt tgcttttcga tttccattga gtgttactgc ttattttctt
       61 ttattatacc cttttttctg aaaaaaagaa aaaaggactt tattttttca aaaatataat
      121 acagtttgaa ataaaatata gactgtttta gaaaagaaag tgtaaaaata gggaattttc
      181 acttgttgaa atcggttact atatggtata cttgtcttat gaatgtaaca gatgactgtt
      241 actagaaaaa agaggacatt aatatggttg ttaagacagt tgttgaagca caagatattt
      301 ttgacaaagc ttgggaaggc ttcaaaggcg tagattggaa agaaaaagca agtgtatcac
      361 gatttgtaca agctaactac acaccttatg atggagacga aagcttcctt gcaggaccaa
      421 cagagcgttc acttcacatc aagaaaattg tagaagaaac taaagcacac tacgaagaaa
      481 ctcgtttccc aatggacact cgtccaacat ctatcgctga tatccctgct ggatttatcg
      541 acaaagaaaa tgaagttatc ttcggtatcc aaaacgatga actcttcaaa ttgaacttca
      601 tgccaaaagg tggtatccgt atggctgaaa ctactttgaa agaaaatgga tacgaaccag
      661 acccagctgt tcacgaaatc ttcactaaat atgtaacaac agttaacgac ggtattttcc
      721 gtgcctacac ttcaaatatt cgtcgcgctc gtcacgcaca cactgtaact ggtcttccag
      781 atgcatactc acgcggacgt atcatcggtg tttacgcacg tcttgctctt tacggtgcag
      841 actacttgat gcaagaaaaa gtaaatgact ggaatgcaat caaagaaatc gatgaagaaa
      901 caatccgtct tcgtgaagaa gtaaaccttc aataccaagc attgcaacaa gttgttcgcc
      961 tgggtgacct ttacggggtt gatgttcgca aaccagcgat gaacgtgaaa gaagcaatcc
     1021 aatgggttaa cattgctttc atggctgtct gccgtgtgat taacggtgct gctacatctc
     1081 taggtcgtgt accaatcgta ttggacatct ttgcagaacg tgaccttgct cgtggtacat
     1141 ttactgaatc agaaatccaa gaattcgttg atgatttcgt tatgaaactt cgtacagtta
     1201 aatttgctcg tacaaaagct tatgaccaat tgtactcagg tgacccaacc tttatcacaa
     1261 cttctatggc tggtatgggt aacgacggtc gtcaccgtgt tactaagatg gactaccgtt
     1321 tcttgaacac tcttgacaac atcggtaact caccagaacc aaacttgaca gttctttgga
     1381 ctgacaaatt gccatacaac ttccgtcgct actgtatgca catgagccac aaacactctt
     1441 ctatccaata cgaaggtgta acaacaatgg ctaaagacgg atatggtgaa atgagctgta
     1501 tctcatgctg tgtgtctcca cttgatccag aaaatgaaga acaacgccac aacatccagt
     1561 acttcggtgc tcgtgtaaac gttcttaaag cccttcttac tggtttgaat ggtggttacg
     1621 acgatgttca caaagactac aaagtatttg atatcgaacc aatccgtgac gaagttcttg
     1681 aatttgaatc agttaaagcg aactttgaaa aatctcttga ctggttgact gacacttacg
     1741 tagatgcctt gaacatcatc cactacatga ctgataggta caactacgaa gctgttcaaa
     1801 tggccttctt gccaactaaa caacgtgcca acatgggatt cggtatctgt ggatttgcta
     1861 acactgttga tacattgtca gctatcaaat acgctacagt taaaccaatc cgtgacgaag
     1921 atggctacat ctacgattac gaaacaatcg gtgactaccc acgctggggt gaagatgacc
     1981 cacgttcaaa cgaattggca gaatggttga tcgaagctta cacaactcgt ctacgtagcc
     2041 acaaactata caaagacgca gaagctacag tatcactttt gacaatcaca tctaacgttg
     2101 cttactctaa acaaactggt aactcaccag ttcacaaagg tgtatacctc aacgaagatg
     2161 gttctgtgaa cttgtctaaa cttgaattct tctcaccagg tgctaaccca tctaacaaag
     2221 ctaaaggtgg ttggttgcaa aacttgaact cactttctag ccttgacttt agttatgcag
     2281 ctgacggtat ctcattgact acacaagtat cacctcgcgc tcttggtaag actcgtgatg
     2341 aacaagttga taacttggta acaatccttg atggttactt cgaaaacggt ggacaacacg
     2401 ttaacttgaa cgttatggac ttgaacgatg tttacgaaaa aatcatgtca ggcgaagacg
     2461 ttatcgtacg tatctctgga tactgtgtaa acactaaata cctcactcca gaacaaaaaa
     2521 ctgaattgac acaacgtgtc ttccacgaag ttctttcaat ggatgacgcc ttggatgcat
     2581 tgagctaatc aagttcttga ataataaaaa ggaaccctcg gtcaaacgac tgagggtttt
     2641 gtgcttggga tagtatgagc aattccttcg gcgcaatatg caatgttttt gggaaggaat
     2701 agtggaccga atctgaaata taatagattg aaactagaat agtacacctt tacttctaaa
     2761 acattcttag
//
LOCUS       DAVBHS010000186         2703 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVBHS010000186 DAVBHS010000000
VERSION     DAVBHS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.186"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(571..1440)
                     /locus_tag="VQ651_001953"
     CDS             complement(571..1440)
                     /locus_tag="VQ651_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET2754899.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(1663..2343)
                     /locus_tag="VQ651_001954"
     CDS             complement(1663..2343)
                     /locus_tag="VQ651_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2754900.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPEIPSPQEPGTPGEPTPEKPIPQPNPEHPSVPTPNPELPNQETPTPDK
                     PTPEPGTPKAETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAGLGLGF
                     FKKKEDEK"
     gene            complement(2301..>2703)
                     /locus_tag="VQ651_001955"
     CDS             complement(2301..>2703)
                     /locus_tag="VQ651_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET2754901.1"
                     /translation="FKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANYYKE
                     GSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPANAY
                     QKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
BASE COUNT          746 a          564 c          524 g          869 t
ORIGIN      
        1 tactatacaa cctcaaaact gtgttttgaa caacctgcgg ctagcttctt agtttgccct
       61 ttgattttca ttgagtataa aattgcagat aaaacgataa caatcatagg actcagcaga
      121 gtcaatgtag gtcgaagaat agccccccta atcgcttatt ttatagcaaa atgaaataga
      181 aactgaacat tttgttgagg aaggtaaatc aattcctcac aatgtttgag aaagaaggga
      241 ggactattcc aacttcaacc cactataaag cagacttaca agatagggaa cccatctccg
      301 ataatatata gtagaatgaa aactgcacaa tttgattgga gaattcaaac caatttctaa
      361 caatgtttta gaaacaaggg tctattattt caactgcaac ctactatagc ccattgaagc
      421 tggaatagcc cactgagact tctaaaccac tgctggagag gagtttgttt agagtttctt
      481 tcatttccct aaaaaatcaa aaagagagaa ccagtctgat cctctcttac ttatagatgg
      541 tgctcactca aatgttaaag gagctatttc ttagagataa aggaatttga atacagctac
      601 tgccgcaatt gctgctgcga taggtgctac tactggtacc caagaatacc accattttga
      661 atcgccttta tgctcaccaa gaactgattt tggaaggaaa gcatgaagga gacgtggtcc
      721 caagtcacgg gctgggttca aggcaggtcc tgtaggtcct ccaagtgatg ttaccaaagc
      781 cataacgagg aatccaagtg ccaagtgagc cacagaaagt cctgaagcag tgtgtggagc
      841 cacctgtgct ttaatagcca agtcagaaaa atcaactgtt tgtcctgctt ctattgcctt
      901 ttgtttcatg aattgaagca cttcagcacc aaagaagttt ttagtcaaac caagagctgc
      961 aaagaacaaa acaaatgaac caacaaactc attaatcaaa ccattgacag ttgctgcata
     1021 gcgactttct tttgtaccat ggtcaatact tgaaatagtt gagaaagttc ccaagatgtt
     1081 atttggtttt tcagttttca agtagaatgg acggtatgtt gccacaacta aggcttggcc
     1141 aaagatagcc cccaagactt gcgcgataat gtaaggtacc acttgtgccc aagggaaaag
     1201 accgctaact gcaagcccta gagtgaaagc agggttgatg tgattcccag atacgttacc
     1261 aaacatcaag gctgggatca taacccccat accataacca acagcgatga cgatccagcc
     1321 actttggtga cctttcgtac ctttaagttc aacgttggca actgcaccat tcccaagaat
     1381 gatcaaaatg gcagttccca aaaattcagt ggcatacttc agtgcccatg tgaaatccat
     1441 ttgctagatt ctccttaaaa ttttttagat attccttatt ttatcaattt ttaccctatt
     1501 tagcaactga tttctggaaa tatttttaaa aaaataggat tcatagtttc ccagttcaac
     1561 gtacttgtaa gcgtttttat tcttcacgaa agacctcatt ctcaaaacca ggaaaaagcc
     1621 cacatctgtg atcttttcct ggttcctaga ttctaaaatc tattattttt catcttcttt
     1681 tttcttgaag aatcctaatc ctagaccagc taacaaggtc atgataccag cactagccaa
     1741 ggtagcatta gcttctgtac ctgtgtttgg caattcctct ctcttacctg tctcataagt
     1801 cggaacttct gggtctggat tcactggagt ttcagctttt ggagtacctg gttctggagt
     1861 tggtttatct ggtgttggag tctcttgatt tggtagttct gggtttggtg tcggaacact
     1921 tggatgctct ggatttggtt gtgggattgg tttttctgga gtcggttcac ctggagtacc
     1981 tggttcctga ggacttggaa tttctggttt ttctggagtt ggtttatctg gtgttgataa
     2041 acggtcatac cttaccgtta tttctttatc actagagtct gacgtaactt cttgtgattc
     2101 aactgttgga atatctggat ctttgtactt gtcaatctta ccagatataa cctcgtccca
     2161 gtttcctgtt gtccattcac cgtaggttac aactcccgtg accttgttct cagtttttgt
     2221 acggcttaag gttacaggtt gaacaacatc ttcttttaca ttttggttcg taactttatc
     2281 aacgtaatga atgatacgcg ttatagtctt cgtctcagta gaggttgctg ttttgggaac
     2341 cactgtttcc tcaacattct cacggtagta atagtcaact gttgcaccgt cttctggtac
     2401 gcatttgcag gagttgctac caaggtgtat gttgttttcc ttgtgataac tcggtcttct
     2461 ttgtcctcag ttgttgtttt cccttcaata gtttttgatt ctgtggtata ctcagaacct
     2521 atcgctaaat cagcttttat aacagactct gccaacttct cttggctacc ttctttatag
     2581 taattggctg ttactgtagc agtggttggc gcttcgcttt ctctataaac taaggtcact
     2641 gttctacctt cgcttacaat attcccagtt aaacttgcag aatttgtatc tgcttcttta
     2701 aaa
//
LOCUS       DAVBHS010000187         2634 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVBHS010000187 DAVBHS010000000
VERSION     DAVBHS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.187"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            195..2354
                     /gene="pfbA"
                     /locus_tag="VQ651_001956"
     CDS             195..2354
                     /gene="pfbA"
                     /locus_tag="VQ651_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HET2754902.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVISTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKESVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            2338..2634
                     /locus_tag="VQ651_001957"
     CDS             2338..2634
                     /locus_tag="VQ651_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754903.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYSKMK"
BASE COUNT         1027 a          323 c          496 g          788 t
ORIGIN      
        1 tctactatag aactgaccaa gtcagtaacc tagacttagg gcaaggcggc actgacctag
       61 tttgaagaga tttccgaaga gtataaattt taatattttc ttgtgttatt ccttgacaat
      121 tcaatttgga aaatatatga taaagataat gacagcggtg tcattctatc tattttaaga
      181 aaagtaataa tcaattgtta aaaatagtaa aaaaattgga ggttctgatg aaatattttg
      241 ttcctaatga ggtattcagt attcgtaaat taaaggtggg gacttgctcg gtactattgg
      301 caatttcaat tttgggaagc caaggtattt tatcggatga agttgttact agttcttcac
      361 cgatggctac aaaagagtct tctaatgcaa tcactaatga tttagataat tcaccaactg
      421 ttaatcaaaa tcgttctgct gaaatgattg cctctaattc aaccactaat ggtttagata
      481 attcgttaag tgttaatagt atcagctcta atggtactat tcgttccaat tcacaattag
      541 acaacagaac agttgaatct acagtaatat ctactaatga aaataagagt tataaggaag
      601 atgttataag tgacagaatt atcaaaaaag aatttgaaga tactgcttta agtgtaaaag
      661 attatggtgc ggtaggtgat gggattcatg atgatcgaca agcaattcaa gatgcaatag
      721 atgctgcagc tcaagggcta ggtggaggaa atgtatattt tcctgaagga acttatttag
      781 taaaagaaat tgttttttta aaaagtcata cacacttaga attgaatgag aaagctacaa
      841 ttctaaatgg tataaatatt aagaatcacc cttccattgt ttttatgaca ggtttattta
      901 cggatgatgg tgcgcaagta gaatggggcc caacagaaga tattagttat tctggtggta
      961 cgattgatat gaacggtgct ttgaatgaag aaggaactaa agcaaaaaat ctaccactta
     1021 taaattcttc aggtgcattt gctattggga attcaaataa cgtaactata aaaaatgtaa
     1081 cattcaagga tagttatcaa gggcatgcta ttcaaattgc aggttcgaaa aatgtattag
     1141 ttgataattc tcgttttctt gggcaagcct tacccaaaac gatgaaggat gggcaaatca
     1201 taagtaagga gagcattcag attgaaccat taactagaaa aggttttcct tatgccttga
     1261 atgatgatgg gaaaaaatct gaaaatgtga ctattcaaaa ttcctatttt ggcaaaagtg
     1321 ataaatctgg ggaattagta acagcaattg gcacacacta tcaaacattg tcgacacaga
     1381 acccctctaa tattaaaatt ttaaataatc attttgataa catgatgtat gcaggtgtac
     1441 gttttacagg attcactgat gtattaatca aaggaaatcg ctttgataag aaagttaaag
     1501 gagagagtgt acattatcga gaaagcggag cagctttagt aaatgcttat agctataaaa
     1561 acactaaaga cctattagat ttaaataaac aggtggttat cgccgaaaat atatttaata
     1621 ttgccgatcc taaaacaaaa gcgatacgag ttgcaaaaga tagtgcagaa tatttaggaa
     1681 aagtatcaga tattactgta acaaaaaatg taattaataa taattctaag gaaacagaac
     1741 aaccaaatat tgaattatta cgagttagtg ataatttagt agtctcagag aatagtatat
     1801 tcggtggtaa agaaggaatt gttattgagg attcaaaggg taaaataacc gttttaaata
     1861 accaatttta taatttatcc ggtaagtata tatcattcat caaatctaat gcaaatggga
     1921 aagaatctgt tatacgtgat agcgatggta atttcaatat tgtaacggag aatgggcttt
     1981 acaaaattgt aacaaataat ttaagtgata aaaacgaaaa agaaaaaaac aaagaggaaa
     2041 aacaatctaa ttcaaataat gtaattgata gtaaccagaa gaacggagag tttaactcaa
     2101 gtaaagataa tagacaaatg aatgacaaga tcgacaataa acaagataat aagacagaag
     2161 aagtaaacta taaaatagtt ggagatggca gagaaactga aaatcatatt aataaatcta
     2221 aagaaatagt agatgtaaaa caaaaattac caaagactgg ttcgaacaag attatggaac
     2281 tattcttaac agtgacagga attggtttac ttttgacact aaaagggttg aagtattatg
     2341 gtaaagataa ataaaatttg ttcgatacaa ggaagttccg tagaaaatga ggatattgta
     2401 ggttcacaaa atcagtattt ttggattatt ggtggagcta cagatttata taattctaaa
     2461 gaggagatag gttattcagt ctcagaagtt gtacatattt tatcagaaag tttgtcggtt
     2521 aattgtaaag aatcaaaaac tcttaaacaa atatttgaaa ctgctttact cgaggttaaa
     2581 gatgaaatcg gtttaaactc atatgaatta acagagtata gtaaaatgaa ataa
//
LOCUS       DAVBHS010000188         2585 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVBHS010000188 DAVBHS010000000
VERSION     DAVBHS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.188"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..841
                     /locus_tag="VQ651_001958"
     CDS             <1..841
                     /locus_tag="VQ651_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET2754904.1"
                     /translation="PEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLEVTYTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSVIEAALQPAAPFVEITKFPAVSRDVALLLKAAVTHQEVIDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEKKVNA
                     EVR"
     gene            complement(1014..2015)
                     /locus_tag="VQ651_001959"
     CDS             complement(1014..2015)
                     /locus_tag="VQ651_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET2754905.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIADDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            2171..>2585
                     /locus_tag="VQ651_001960"
     CDS             2171..>2585
                     /locus_tag="VQ651_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2754906.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSLEMMVMAKGVNVGISTIYYWIHRGKLGLSKQDL"
BASE COUNT          845 a          464 c          540 g          736 t
ORIGIN      
        1 tcctgaaaaa gcagttgagt ttacggctca accaagtaac cttacggaac tcatgtggcc
       61 aatgactgtg gatcgttcag tcctccgtca aaatatgatt tcaggtatcc ttgataccgt
      121 tgcctacaac gtggctcgta agaataaaaa cttggccctt tacgagattg gaaaagtctt
      181 tgaacaaaca ggtaatccaa aagaagaact tccaaatgaa atcaacagtt ttgcctttgc
      241 cttgacaggc ttggttgctg aaaaagattt ccaaacagca gcagttccag ttgatttctt
      301 ctatgctaaa ggaatcctag aagcgctctt tactcgcttg ggactagaag tgacctatac
      361 agcaacagct gagattgcca gccttcaccc aggacgtaca gcagtcattt cactcggtga
      421 ccaagttctt ggttttcttg gccaagtgca tccagtcact gctaaggctt acgatattcc
      481 agaaacgtat gtggctgagc tcaacctttc agtcatcgaa gctgcccttc aacctgctgc
      541 tccatttgtg gaaatcacga aattcccagc agtcagccgt gacgttgccc ttctcctcaa
      601 ggcagcagta actcaccaag aagttataga tgctatccaa gctgccggcg tgaaacgttt
      661 gacagatatc aaactctttg acgttttctc aggtgagaaa ttgggacttg gtatgaagtc
      721 aatggcttat agcttgacct tccaaaatcc agaagacagc ttaacggacg aagaagtcgc
      781 acgctacatg gaaaaaatcc aagcatcgct tgaaaaaaaa gtgaacgcag aagtgcgtta
      841 aacgatagta ggatacaaat tccccctcta gtttatgaac actagagggg tgattttata
      901 taatactcaa tgaaaatcaa aaagcaaact aggaagctag ccgtaagctg tacttgagta
      961 ctgtaaggcg aggctgacgt ggtttgaaga gattgtcgaa gagtataaaa taattaaaat
     1021 gtgatttctg taaaaataca tctatgatca gagttttgaa aacttacaat gtctaaatct
     1081 ttaacatagt agtggttttt aggcaataaa atatgatcta ttgttgcatt aaaaagtttt
     1141 ggactttggc tattccaagt tcctctttca aaaggtggta gtacatttaa tgcgtcccta
     1201 tgagagctta tttttgcaag tgctccatga cgcatagttg cattaaaatc atctgcaata
     1261 atagcctttg gatattttga agccaattga ttatgaatga tgtttaagtc ttgcttccag
     1321 atttccatta aacctggcag aggaggagca gtatgcaagg caatgatatc tggtatattt
     1381 tgttttctcg aatgtaatac aattgtcccg aaccgtgttg tatgaaaagt tttagcttct
     1441 gtatagaaac cataactttt cttgacaatc acagtcacag gagctattcc actattggta
     1501 ggtggagaag tgaaaatatc atagttggcc atagaaagtc caacttgatg aaacaatagt
     1561 ttgattctct ggttttcttg ctcacctctg atattggtag ctagttcagg gaatatagcc
     1621 atatcggcgt caaaatggct aaaaattcgc tcaatatgtt gtgcttctat ttgattagcg
     1681 acgttccaag ttactaattt taaggtttta gtcttatttg atgcagaaga aagggtttta
     1741 tctgttccga ataatagtaa tatgagcgat agtaagagca aaatgccaga gtaaaaccgt
     1801 atttgtttat ttttcttgta aaaagatagg gaaaaaaata cgaaacttaa aatagcagcc
     1861 atagttgcag tagctaaggg gaaggcataa atctgtgcca agataggtct ggtagatagc
     1921 caaatattaa gattagaaga tggaaaaaaa gaataagtta tgatagggat aacaaagaga
     1981 atgaccgaaa gggcagagag aaatgtttta atcattgtgt gagtacctca atttgcaatg
     2041 gttgctcgga gctaatgtga ctttgtgact ttacttgatt gatattcttt ttcttgtctt
     2101 cctcatagat taagtacaac ccaagtaaaa aaagtccaat ttaagattga aatgattaaa
     2161 aggcacagaa atgcaagaca attatactac aaaaggcaaa catttgacaa tcgatagccg
     2221 tcgcttaatc gaaagatgga aaaaagaagg gaaatcaaat agagaaattg cctctctgct
     2281 tggaaaagtt cctcaaacta tccacactga aatcaagcgt gggacagtcc gacaatgtct
     2341 tggaaaaggg cgcttcaaag aggtttattc tgccgactat gctcaacagt cttatgaaaa
     2401 caatcgcaag cgctcggtca agaaatcaag cttgaccaag gaactaaagg aaaagattct
     2461 ccactatcat aaccaaaaat tttcgcttga aatgatggtg atggctaaag gggttaacgt
     2521 gggaatttca accatttact attggattca tcgtggaaaa ttggggttaa gcaagcagga
     2581 tctgc
//
LOCUS       DAVBHS010000189         2570 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVBHS010000189 DAVBHS010000000
VERSION     DAVBHS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.189"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(478..703)
                     /locus_tag="VQ651_001961"
                     /pseudo
     CDS             complement(478..703)
                     /locus_tag="VQ651_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1551..2423
                     /locus_tag="VQ651_001962"
     CDS             1551..2423
                     /locus_tag="VQ651_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HET2754907.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
BASE COUNT          763 a          442 c          500 g          865 t
ORIGIN      
        1 agatatgcac aaattgatta ggaaattcaa atgaatttct acaaatgttt tagcaatcgt
       61 aatgtacttg tctagattcg atctgatata ttttcgattt aatgatatgg tatttaaaac
      121 ctccaaagta gcttgctcca ttcttttact tacgtgagtg tagatgttat ttactgtttt
      181 agcgtttttg tgttccactc taaccattat agcattcttc tcagctagtg tactaaggag
      241 tgtgtgcctg aaaatatggg aactaagggg ctggtttatc ggtttctcta gtttagtatt
      301 tgccttttgc aaagtgatgt taaatgcctt tctctaaatt tacatatcac tattgtttaa
      361 caaaatctaa tctactttag gtcacttatt ctttttttga aatgtagaat gaactttttc
      421 aaagtttttc gaatctttta aaatctgttt gttttatatc gccattctcc ccctttttta
      481 attctcccta tatagcctga caactttccc gatggtacga atatggttgc tttcgtctag
      541 gtggatgtcg gggtatttgg gattgagttt ttttaaggta gccttggcgg agtttcttga
      601 catagttagc gccgtctact tggaagatgc cgatggtatt atagtcaatc tgtggggtat
      661 tcttgataaa taggtagtcg ctgtttctta tctttggctc catggacttg ctgacgacat
      721 aagcgattgg gtcgtagtcg tctgggataa tggaaactcc ataactaaat cgtcgtcctg
      781 catcgagcgg ctacctgcag agataaacta cctaacacga gagtaagtag tctgtctgta
      841 gtcgtccagt ctgatgattt ttacgatatt tcgtttttct gctcatacag ttgcctctcg
      901 gcataggtca gaactttacc ttgtctgggt ggtgcccgtt ggtcgtagat agattggata
      961 tcgctaggag aatccttttg aactggagga aagagggtat caatcaagct actgaatact
     1021 ttagctaagt caaatatagt atttttctta gtagacctaa cccttttttc ataatttcca
     1081 atggtgtttt tacttatacc aatcttagta cccaattctt attgagtcca accattacta
     1141 gtctatattg ttttatagtt gattgagttt ggaatagtac gctgtagctg ctaaaacatt
     1201 tctaggaatt aatttgactt tcctaataga gttgttcata tcttatttca atctactatg
     1261 tttttcacct ctaacaatcg caatctcttc tttatccatg aatgaaatcg ctttctattt
     1321 ttgtaagtaa agcatcacac gaaatccacg aaaatgaaaa cctttgttgt gttttcgtaa
     1381 aaaatttgtt gacagagcac gaaacgccta ctataattaa atcaagctta aggaaataac
     1441 aaaaaaacaa atcggaggga aagacgatga acacattaaa tgagaaagta atcaatatct
     1501 gtaaagcagt agttaaagaa actttaatcc aagacattta ggagaaagaa atgaagaaat
     1561 caagaaaatt agccacatta ggaatctgtt cagccttgtt tttaggcttg gctgcttgtc
     1621 aacaacaaca tgctacttct gaggggacga atcaaaggca aagcagttca gcgaaagttc
     1681 catggaaagc ttcatacacc aacctaaaca accaggtaag tacagaagag gtcaaatctc
     1741 tcttatcagc tcacttggat ccaaatagtg ttgatgcatt ttttaatctc gttaatgact
     1801 ataataccat tgtcggctca actggcttat caggagattt cacttccttt actcacaccg
     1861 aatacgatgt tgagaaaatc agtcatctct ggaatcaaaa gaagggcgat ttcgttggga
     1921 ccaactgccg tatcaatagt tattgtcttt tgaaaaattc agtcaccatt ccaaagcttg
     1981 aaaagaatga ccaattgctt ttcctagata acgatgcgat tgataaagga aaggtctttg
     2041 attcacaaga taaggaagag tttgatattc tattttcgag agttccaact gaggcaacta
     2101 cagatgtcaa ggttcacgct gaaaagatgg aaacattctt ctcacaattt caattcaatg
     2161 aaaaagctcg aatgctgtct gtagtcttgc acgacaatct ggatggcgag tatctgtttg
     2221 taggccatgt tggggtctta gtacctgctg atgacggttt cttatttgta gagaaattga
     2281 ctttcgaaga gccctaccaa gcgattaaat ttgctagtaa ggaagattgc tacaagtatt
     2341 tgggcaccaa gtatgcggat tatacaggcg agggactggc taagcctttt atcatggata
     2401 atgataagtg ggttaaactt taatagtgtt atgatatgac agtaatctta ggatttttat
     2461 ttctattttt gatgacttgg tgggttattt caatcgttaa tagccagaaa ccacgcaaaa
     2521 atgaaacatt tattggctat atccaaagat acgatattga tgggaatggc
//
LOCUS       DAVBHS010000190         2561 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVBHS010000190 DAVBHS010000000
VERSION     DAVBHS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.190"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            166..480
                     /locus_tag="VQ651_001963"
     CDS             166..480
                     /locus_tag="VQ651_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754908.1"
                     /translation="MWVLGFILFLIFFYSNDSKKIKKLEKKIKRLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            490..696
                     /locus_tag="VQ651_001964"
     CDS             490..696
                     /locus_tag="VQ651_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2754909.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            696..893
                     /locus_tag="VQ651_001965"
     CDS             696..893
                     /locus_tag="VQ651_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754910.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            949..1629
                     /locus_tag="VQ651_001966"
     CDS             949..1629
                     /locus_tag="VQ651_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HET2754911.1"
                     /translation="MGMFVGMFKARVESHEIILDVKALMPWISAICLLIGFISMFLTF
                     NFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITGVVIPLAIFISLSEV
                     IILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFATPKDVLNYINSYDEG
                     ERQANLEQSFRILFQLHQYVLPALYIFLIILSFLTGEIQLLAFLLVGAIHVYINVMQL
                     PMVKRYFK"
     gene            1641..2282
                     /locus_tag="VQ651_001967"
     CDS             1641..2282
                     /locus_tag="VQ651_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2754912.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            2300..2530
                     /locus_tag="VQ651_001968"
     CDS             2300..2530
                     /locus_tag="VQ651_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754913.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
BASE COUNT          742 a          422 c          526 g          871 t
ORIGIN      
        1 tttagaagag tattaatgat agataaagtg gtcaggaacc tactcctgac ctttttcttt
       61 tgcaaaatga caaaaatcat aatttttttg acaactatac ttgtcaaaaa gaaaaagatg
      121 tgttacaatg taagcaagtt aagagaaaag aagaaaggag ctatgatgtg ggtactaggg
      181 tttatactat ttctgatttt cttttattct aatgattcta aaaaaatcaa gaaactagag
      241 aagaaaatca aaagacttga gcgaaaagag aaaggaaatg cagaaatgtc gagattatta
      301 caagaaatga ttggaaagga accaattata acgggagtgt atattgggcc agataactgg
      361 gaagttgtgg atgttgatga ggaatgggta aagctacgac gtgtagataa tacgggaaaa
      421 gaaaaattca agttgcaacg tattgaggat atccaaaccg ttgaatttga cggagagtag
      481 gtgtgtaaca tgcaactaaa aaatcgtcta aaagagcttc gagctcgcga tggtctcaat
      541 caaaccgacc tagccaaact ggcaggggtt tccagacaga ccattagcct actagaacgg
      601 gatgagtaca ccccatccat tatcattgcc ctgaaaatat ctcaaatttt caacgaaaca
      661 gtcgaatcgg tatttcgttt ggaggaggac gagtgatgaa caagtataaa gtgatttatt
      721 atgtagtggt catagcttta ttagtcagcg tatttctact gattgggatg gacctaagct
      781 ggtttaatcc ctatcaaagc gaccaatttg tttgggtcta ctttgctctc atcccagtaa
      841 ttgaatggat tgaaaagaaa tccaaaaatc tagcaagtga aaaaggagaa tgaatatgaa
      901 aaagaaacgt agattgttat ttttgatgtc tattgtcttg gggggttctt gggcatgttt
      961 gtagggatgt ttaaggcacg tgtcgaatcc cacgaaatta ttttagatgt aaaagccttg
     1021 atgccatgga tatcagctat ttgtttactg ataggtttca ttagtatgtt tttgactttc
     1081 aatttcttaa agaaaagcag aaaatttcat tccttgtatc aagaggaaat ggatgacgat
     1141 ctgaatgaaa cctattatgt gcaaatgtat cggaatcttg agtttggaac cattgctttt
     1201 aatattacag gtgtagtgat tccattggct atttttattt cattaagtga ggtgattata
     1261 ttgcatacaa accctcaaac atttttcctt tctttcttac tctttgtggt attcttagtc
     1321 gctcaaaaat ctctttttaa aaccattgcg attgttcgtc agtttgattt ggaatttttc
     1381 gctacaccaa aggatgtctt gaactatata aattcttatg atgaagggga gcgtcaggct
     1441 aatttggaac agagttttcg aattttattc caattacacc aatatgtctt accagcctta
     1501 tatatttttc ttattatcct ttctttcttg acaggagaga ttcagttact agctttcttg
     1561 cttgtaggag ccatccatgt ttatatcaat gtgatgcagt tacctatggt aaaacgttat
     1621 ttcaaataaa ggagttatct atgaaattac ttaaaaacct tggctggttt cttctagccc
     1681 ttctatcctt tttctttatc tatggcttta ttcaggggct cgcgactgcg tcgcttgctt
     1741 taggcgcttc accctatgct gttactctac tctatgtggt cttggctgga gtatatgtgt
     1801 acggcattta caaatggtat cagaaagctc ctgtttatat tgagaagagc ggctttaacc
     1861 gatttatttg gcttcctgcc ttggtttggt tcttatctct ggtcgttcaa ttcttcttgc
     1921 cagatgatcc ttcagtaaat cagcaaatag cgacagactt gaccttgtct caaccacttt
     1981 tctcattctt tgctgtggtt atttttgctc ctttgacgga agagattgtt tttagaggga
     2041 tgttagcacg ctatctcttt cctaagcagg acaatagtaa acgaaccctg atttttcttc
     2101 tggtatctag tcttctattt gccttgattc attttccagg tgatgtgcaa caattttttg
     2161 tctattttag ccttggtttt agtttgggtt tggcttacat tagcagaaaa ggtctggtct
     2221 acagtatttc tcttcacgct ttgaataatt tagtcggctt tttgatgatt ctcatgctat
     2281 aatagagtca ggaggtcaca tgaaacgagt aattttatta gcagtgattc aggcagtcgt
     2341 tctatttttc atcattggag cgctagctta tgccttcaaa ggcgatttct tctataacta
     2401 tctagcagtt gtctttgctc ctattgcagg tgtactgcgt tttgggacgg cttacataac
     2461 ggaaattgtc ttgcctcgaa aggcagccga aatcgctgaa aagcgtaaag caggcaaaaa
     2521 ttcaaaataa aagagtccgg tgaagttcat cggatttttg t
//
LOCUS       DAVBHS010000191         2556 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVBHS010000191 DAVBHS010000000
VERSION     DAVBHS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.191"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            344..2449
                     /locus_tag="VQ651_001969"
     CDS             344..2449
                     /locus_tag="VQ651_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2754914.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELERKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQEKVIGQDKAVEVVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVVIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
BASE COUNT          822 a          467 c          588 g          679 t
ORIGIN      
        1 aaggcgaagc tgatgtggtt tgaagaggtt ttcgaagagt gtaaaaaaat attggtcagt
       61 taaagtcaaa actcttgacc ttttctgacc aatgtagtat attatgaacg taaggtaaat
      121 gaaagcctta ctagaacact tatttaaagt aaaatagaaa gaggtgagtc tatgtttcat
      181 ctagcaatct tcagaggtaa agatagtcta ctcctgcttg aaaaagaaaa accagaaata
      241 gtacgtaggg tagcgatcta gctagtctaa attttttaaa acaatggtca aagatagtca
      301 atatcagtaa tcataactaa gtaaacaaaa agaggtaaag aatatgaata acaactttaa
      361 taattttaat aacatggatg atttatttaa ccaattgatg ggtggtatgc gaggatatag
      421 ttctgaaaat cgccgttact taattaatgg acgcgaagtc acacctgagg aatttgctca
      481 ctatcgtgcg actggtcaat taccaggaaa tgcagaaact gatgtgcaaa tgccacaaca
      541 ggcatcaggt atgaaacaag acggtgtcct tgcaaaacta ggtcgaaact taacagcaga
      601 agcgcgtgag ggcaagttgg atcctgttat cggacgaaac aaggaaattc aagaaacatc
      661 tgaaatcctc tcacgccgca ccaagaacaa tcctgttttg gtcggagatg caggtgttgg
      721 taagacagca gttgtcgaag gtctagcgca agccattgtg aacggagatg ttcctgctgc
      781 tatcaagaac aaggaaatta tttccattga tatctcaggt cttgaggctg gtactcaata
      841 ccgtggtagc tttgaagaaa atgtccaaaa cttagtcaat gaagtgaaag aagcagggaa
      901 tattatcctc ttctttgatg aaattcacca aattcttggt gctggtagca ctggtggaga
      961 cagtggttct aaaggacttg cggatattct caagccagct ctctctcgtg gagaattgac
     1021 agtgattggg gcaacaactc aagacgaata ccgtaacacc atcttgaaga atgctgctct
     1081 tgctcgtcgt ttcaacgaag tgaaggtcaa tgctccttca gcagagaata cttttaaaat
     1141 tcttcaaggc attcgtgacc tctatcaaca acaccacaat gtcatcttgc cagacgaagt
     1201 cttgaaagca gcggtggatt attctgttca atacattcct caacgtagct tgccagataa
     1261 ggctattgac cttgtcgatg taacggctgc tcacttggcg gctcaacatc cagtaacaga
     1321 tgtgcatgct gttgaacgag aaatcgaaac ggaaaaagac aagcaagaaa aagcagttga
     1381 agcagaagat tttgaagcag ctctaaacta taaaacacgc attgcagaat tggaaaggaa
     1441 aatcgaaaac cacacagaag atatgaaagt gactgcaagt gtcaacgatg tggctgaatc
     1501 tgtggaacga atgacaggta tcccagtatc gcaaatggga gcttcagata tcgaacgttt
     1561 gaaagatatg gctcatcgct tgcaagaaaa ggtgattggt caagataagg ccgtagaagt
     1621 tgtagctcgt gctatccgtc gtaaccgtgc tggttttgat gaaggaaatc gcccaatcgg
     1681 cagcttcctc tttgtagggt ctactggggt tggtaagacg gagcttgcta agcaattggc
     1741 actcgatatg tttggaaccc aggatgcgat tatccgtttg gatatgtctg aatacagtga
     1801 ccgcacagct gtttctaagc taattggtac aacagcaggc tatgtgggtt atgatgacaa
     1861 tagcaatacc ttaacagaac gtgttcgtcg caatccatac tctatcattc tcttggatga
     1921 aattgaaaag gctgatcctc aagttattac ccttctcctc caagttctag atgatggtcg
     1981 tttgacagat ggtcaaggaa atacagtaaa cttcaagaat actgtcgtta ttgcgacctc
     2041 aaatgctgga tttggctatg aagccaactt gacagaagat gcggataaac cagaattgat
     2101 ggaccgtttg aaacccttct tccgtccaga attcctcaac cgctttaatg cagtcatcga
     2161 gttctcacac ttgactaagg aagacctttc taagattgta gatttgatgt tggctgaagt
     2221 taaccaaacc ttggctaaga aagacattga cttggtagtt agtcaagcgg ctaaagatta
     2281 tatcacagaa gaaggttacg acgaagtcat gggggttcgt cctctccgtc gcgtggttga
     2341 acaagaaatt cgtgataagg tgacagactt ccacttggat catttagatg ctaaacatct
     2401 ggaagcagat atggaagatg gcgttttggt tattcgtgag aaagcctaag atagaatttt
     2461 gaggataaaa aagaaggagc cagctgaaaa aaactggttc ctttttaggt acgacaggca
     2521 tgtcgtatag tagaagtgta ttattccagt ttcaat
//
LOCUS       DAVBHS010000192         2506 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVBHS010000192 DAVBHS010000000
VERSION     DAVBHS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.192"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            357..1703
                     /gene="gdhA"
                     /locus_tag="VQ651_001970"
     CDS             357..1703
                     /gene="gdhA"
                     /locus_tag="VQ651_001970"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HET2754915.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
     gene            complement(2209..2379)
                     /locus_tag="VQ651_001971"
     CDS             complement(2209..2379)
                     /locus_tag="VQ651_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754916.1"
                     /translation="MKKENEYVILTIASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
BASE COUNT          806 a          467 c          433 g          800 t
ORIGIN      
        1 ctttagttga tttcatatca ctattatata atgatttttg attttagtct ggtataaata
       61 ttgctttcct ccaaaatggt cataatttta ctagcaaatc taacatatca cggataaatt
      121 aacaagtgat ttctgaattg ctaaacattt tcttttctta tagcatacca tactttaaga
      181 ttttgtcttt gagaaagata tttccaagaa aaacgttcgt ttttttatct attttcaaaa
      241 aaaccaaaaa ctgaattgaa agaattttta gaaaatttct gttttttctt tacagtgaaa
      301 aaaaattctg ctataatgtc ttatgtaata aaatatgaca ataaaaggag aacgatatga
      361 catctgctaa agaatatatc caaagcgtgt ttgaaactgt gaaagctcgt aacgggcacg
      421 aggctgaatt cctccaagct gttgaagaat ttttcaacac tttagaacct gtatttgaaa
      481 aacaccctga gtatatcgaa gaaaatatct tggcacgtat tactgagcct gagcgcgtgg
      541 tttctttccg tgttccttgg gttgaccgtg atggaaaaat tcaagtaaac cgtggttacc
      601 gtgttcaatt caactcagct gttggaccat acaaaggtgg acttcgtttc cacccaactg
      661 taaaccaagg gatcttgaaa ttcctcggat ttgaacaaat ctttaaaaat gtcttgactg
      721 gacttcctat cggtggaggt aaaggtggat cagacttcga tcctaaaggt aaaacagatg
      781 ctgaagtgat gcgcttctgc caaagcttca tgactgaatt gcaaaaacac atcggaccat
      841 cacttgacgt acctgctggt gatatcggtg ttggtggacg tgaaattggt tacctttacg
      901 gtcaatacaa acgtcttaac caatttgatg ctggtgtctt gactggtaaa cctcttggat
      961 ttggtggtag cttgattcgt ccagaagcaa ctggttacgg tttggtttac tatactgaag
     1021 aaatgctcaa agctaacggt aacagctttg ctggtaagaa agtcgttatt tcaggttctg
     1081 gtaacgttgc tcaatatgct cttcaaaaag caactgaact cggtgcaact gttatttctg
     1141 tttctgactc aaatggttat gtcatcgatg aaaatggtat cgacttcgat cttttggttg
     1201 atgttaaaga aaaacgtcgt gctcgtttga ctgagtatgc agctgaaaaa gcaactgcaa
     1261 cctaccacga aggtactgta tggacttacg ctggaaacta tgacattgct cttccatgtg
     1321 ccactcaaaa cgaaatcaac ggtgaagcag ctaaacgttt ggttgctcaa ggcgttatct
     1381 gtgtatctga aggtgccaac atgccgagcg accttgatgc catcaaagtt tacaaagaaa
     1441 atggtatctt ctacggacct gcaaaagctg ccaacgctgg tggtgtagcc gtttcagctc
     1501 ttgaaatgag ccaaaacagt cttcgcctct catggactcg tgaagaagtt gatggccgtc
     1561 tcaaagacat catgacaaac atcttcaaca cagctaaaac gacttcagaa acatacggtc
     1621 ttgataaaga ctaccttgca ggagctaaca ttgctgcctt tgaaaatgta gcaaatgcca
     1681 tgattgcaca aggtattgtt taagatttat ttactcaatc ctcaatcact acgattgggg
     1741 atttttagct tgaattaaaa aattggaaaa tctaattaaa taaaatgaaa tgaaataaaa
     1801 actgaacaaa ttgattgcgg aattcaaacc aatttctaac atacttctag aaattaattt
     1861 gacttttcta atcgatttgt tcatatctta tttcaattta ctatacttac ttaacccttt
     1921 ttatactcaa tgaaaatcaa agagcaaact aggaagcgag ccgcaggctg tacttgagta
     1981 cggcaaggca aagctgacgt ggtttgaaga gattttcaaa gagtattaaa caaaaagaaa
     2041 aaccgctact cctaagaata gcgatttaat catgttttta agctactact gtagttgctc
     2101 tattatttaa gtgttatgaa taacttcaat atctttaaag ctttttgtta gatttataat
     2161 ttcaactata gccatctcta ctccttagtt tacagcaaaa aattatgctt aattcttatt
     2221 gtttttgtaa acaatcagcg aacccaatac tattccattc aacaagccta aagaaatacc
     2281 atattcaact ggaaaatcta aaaaaattgc aaacactatt ccaatcatca cccctagtga
     2341 ggctattgtt aaaattacat attcattttc tttcttcatt ttctttacac ctcatttcct
     2401 ttttgtatat aagctgtagt actctccacc aagagttatt aagtacttat gatttcctga
     2461 ctcaacaatc ttaccacctt tcattacaaa aataatatcc gcatcc
//
LOCUS       DAVBHS010000193         2380 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVBHS010000193 DAVBHS010000000
VERSION     DAVBHS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.193"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..952
                     /locus_tag="VQ651_001972"
     CDS             <1..952
                     /locus_tag="VQ651_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HET2754917.1"
                     /translation="YYAIFCFAGSLIISNPAIALGAQATFILLNIFIVSRGVQKGIER
                     ASKVMMPLLFIIFVVIIERSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALRQSFFALS
                     LGVTAMLTYASYLDKKTNLVQSRISIVAMNISVSIMAGLAIFQARSPFRLNIEGGPSL
                     VFIVLPQLFDKMPFGTIFYVLFLFATVTSSVVMLEINVDNITNQDNSKRAKWSVILGI
                     LTFVFGIPSALSYGVMADVHIFGKAFFDAMDFLVSNLLMPFGALFLSLFTGYIFKKAL
                     AMEELHLDERAWKQGLFQVWLFLLRFVIPIIIVVFIAQFM"
     gene            complement(992..1936)
                     /gene="manA"
                     /locus_tag="VQ651_001973"
     CDS             complement(992..1936)
                     /gene="manA"
                     /locus_tag="VQ651_001973"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HET2754918.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTILVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVESWTLEGQDLELIVSHP"
     gene            complement(2068..2349)
                     /locus_tag="VQ651_001974"
     CDS             complement(2068..2349)
                     /locus_tag="VQ651_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET2754919.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
BASE COUNT          610 a          562 c          448 g          760 t
ORIGIN      
        1 ctactatgcc atattttgtt tcgcaggaag tctgatcatt tcaaatccag ccattgctct
       61 aggagctcaa gcgaccttta tcctattgaa tatcttcatt gtatcacgtg gggttcaaaa
      121 agggattgaa agagcttcga aagtcatgat gcccctgctc tttatcatct ttgtcgtcat
      181 catcgaacgc tctctcagtt tgccaaatgc catggaaggg gttctttact tcctcaaacc
      241 agatttttca aaactgacta gcgctggtct cctctatgct ctgagacaat ctttctttgc
      301 cctctcacta ggggttacag ccatgttgac ctatgcttct tacttggaca agaaaaccaa
      361 tctggtccag tcaagaatct ccatcgtagc catgaatatc tcggtatcca tcatggcagg
      421 tctagccatt ttccaagctc ggtccccctt cagactgaat attgaagggg gaccgagctt
      481 ggtctttatc gtcttgcctc aactctttga caagatgcct tttggaacca ttttctatgt
      541 cctcttcctc tttgcgactg tcacttcttc tgtcgttatg ctggaaatca atgtagacaa
      601 tatcaccaat caggacaaca gcaagcgtgc taaatggagt gttattttag gaattttgac
      661 ctttgtcttt ggcattcctt cagccctatc ttacggtgtc atggcggatg ttcacatttt
      721 tggtaaagcc ttctttgacg ctatggactt cttggtttcc aatctcctca tgccatttgg
      781 agctctcttc ctttcacttt ttacaggcta tatctttaaa aaggctcttg caatggagga
      841 actccatctc gatgaaagag catggaaaca aggactgttc caagtctggc tcttccttct
      901 tcgtttcgtc attccaatca tcatcgtggt cttcatcgcc caatttatgt aatcaaaagg
      961 acttgagtaa tgaactcagg tcctttcttt tttatggatg actaacaatt aattctaaat
     1021 cttgcccttc tagagtccaa gattcaacat cacttggtag gataaagtgt ctcccttttt
     1081 ggattggata gtttttcccg tcaacagtta gttgaccttg accagccaag acactcaaca
     1141 agctgtagtc agctgtcttt tcaaagttaa cttttccagt aatttcccat ttgtaaactg
     1201 cgaagaaatc attggataca aggatagtgg aacgtagatc atctgctttg attgtcactg
     1261 gacggctatt tgcgggttca ccaatgttca agacatcgat agatttttca aggtgaagtt
     1321 cacgtaaatt gcccttgtca tccttacggt caaagtcata gacacggtaa gtggtatcgc
     1381 tagactgctg ggtttcaaga atcaagatac ccgccccgat agcgtgcatg gtaccgcttg
     1441 gtacatagaa gaaatctcct gctttaactg gcacttttgt caacagcgca tcccagttct
     1501 tatcctcgat ttgctggcgg agttcttctt ttgacttggc attgtgaccg tagataatct
     1561 ctgaaccttc atctgctgca atgatgtacc aacattctgt ttttccaagt tcgccttcat
     1621 gctcgagtcc ataagcatcg tctgggtgaa cttggacact gagccagtcg ttggcatcga
     1681 ggatcttggt caaaagtgga aatacaggtt ctggacgatt gccaaataat tcacggtgtt
     1741 ccgcatacaa agtagcaaga tctgttccct cgtaacgacc attggcaact ttagaaacac
     1801 catttagatg agctgagata gcccagtact ctccgacttt ttcacttggg atgtcgtagc
     1861 caaactcatc acgtagcttg gtaccacccc agattttttc ttgcataact gattgtaaaa
     1921 ataatggttc tgacatctta atctcctgtc tgatttttct cccctcatta tagcaaaaaa
     1981 agagttccat ttgaactctt ttttacatct tataaagcag ggagaagatt ttataaaaat
     2041 agtaaacaaa tgtgctctac tcgatgctta caccattgct ataaatgaca tgtaccaata
     2101 gaaggacttc ttcttgctac gtttgagagt cccatttcct acattatctc gatctacata
     2161 gataaagcca tagcgcttat tcatttcccc tgtgccagct gaaaccgaat cgatacagcc
     2221 ccatgtcgta taaccaagca agtcaacccc gtctttgtaa atggcatctc gcatggcctt
     2281 gatgtgggcc tctaagtaag taatccgata gtcatctgct acataaccat tctcatccgg
     2341 tgtatccata gcaccgagtc cattttctac gataatacta
//
LOCUS       DAVBHS010000194         2376 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVBHS010000194 DAVBHS010000000
VERSION     DAVBHS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.194"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            55..771
                     /locus_tag="VQ651_001975"
     CDS             55..771
                     /locus_tag="VQ651_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET2754920.1"
                     /translation="MGAFVAFYLWKAVFDSSQESLIQGFSMADITLYIIMSFVTNLLT
                     RSDSSFMIGEEVKDGSIIMRLLRPVHFAASYLFTELGSKWLIFISVGLPFLSVIVLMK
                     IISGQGIVEVLGLTVIYLFSLTLAYLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAF
                     MSGSLIPLAFFPKVVSDILSFLPFSSLIYTPVMIIVGKYDASQILQALLLQFFWLLVM
                     VGLSQLIWKRVQSFITIQGG"
     gene            773..1558
                     /locus_tag="VQ651_001976"
     CDS             773..1558
                     /locus_tag="VQ651_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2754921.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            1597..1875
                     /locus_tag="VQ651_001977"
     CDS             1597..1875
                     /locus_tag="VQ651_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HET2754922.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTRFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            1860..2057
                     /locus_tag="VQ651_001978"
     CDS             1860..2057
                     /locus_tag="VQ651_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2754923.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
BASE COUNT          608 a          428 c          500 g          840 t
ORIGIN      
        1 caggagttga ttacttaccg agtcaacttt attctctatc ggattggcga tgtcatgggg
       61 gcttttgtgg ccttttatct ctggaaggct gtctttgatt cttcgcaaga gtctttgatt
      121 cagggcttca gtatggcgga tatcaccctc tacatcatca tgagttttgt gaccaatctt
      181 ctgactagat ccgattcgtc ctttatgatt ggggaggagg tcaaggatgg ctccattatc
      241 atgcgtttgt tgcgaccagt gcattttgcg gcctcctatc ttttcaccga gcttggttcc
      301 aagtggttga tttttatcag cgttggcctt ccatttttaa gtgtcattgt cttgatgaaa
      361 atcatatcgg gtcaaggtat tgtagaggtg ctaggattaa ctgtcattta tctttttagc
      421 ttaacgctcg cctatctgat taactttttc tttaatattt gctttggatt ttcagccttt
      481 gtgtttaaaa atctttgggg ttccaaccta cttaagactt ccatagtggc ttttatgtcg
      541 gggagtttga ttcccttggc attctttcca aaggttgttt cagatattct ctcctttttg
      601 cctttttcat ccttgattta tactccagtt atgatcattg ttggaaaata cgatgccagt
      661 cagattcttc aggcactcct tttgcagttc ttctggctct tagtgatggt gggattgtct
      721 cagttgattt ggaaacgggt ccagtccttt atcaccattc aaggaggtta gtatgaaaaa
      781 atatcaacga atgcatctga tttttatcag acaatacatc aaacaaatca tggaatataa
      841 ggtggatttt gtggttggtg tcttgggagt ctttctggct caaggcttga atctcttgtt
      901 tctcaatgtc atctttcaac atattccatt cctagaaggc tggacctttc aagagatagc
      961 tttcatttat ggattttcct tgattcccaa gggaatggac catctctttt ttgacaatct
     1021 ctgggcacta gggcaacgcc tagtccgaaa aggggagttt gacaagtatc tgactcgtcc
     1081 catcaatcct ctctttcaca tcctagttga aacctttcag attgatgcct tgggtgaact
     1141 cttagtcggt ggtattttat tgggaacaac agtgaccagc attgtttgga ctcttccgaa
     1201 attcctgctt ttcctagttt gtattccttt tgcgaccttg atttatactt ctcttaaaat
     1261 cgcaacagcc agtatcgcct tttggactaa gcagtcaggc gccatgattt acatcttcta
     1321 tatgttcaat gactttgcta agtatccgat ttctatttac aattctcttc ttcgttggtt
     1381 gattagcttt atcgtgcctt tcgcctttac agcctactat ccagctagct atttcttaca
     1441 ggaaaaggat gtgttcttta acgtaggagg tttgatgttg atttctctgg ttttctttgt
     1501 tatttccctt aaactttggg ataagggctt agattcctac gaaagtgcgg gttcgtaaaa
     1561 gctaaagtaa gactaaaatc aagaaagaaa cttatgatgt ttgtaattga agaagtcaag
     1621 gatgaaaatc aaaaaaaggc agttgtagct gaggttttga aggatttgcc agaatggttt
     1681 ggaatcccag aaagcacaca agcctatata gaaggaacca cgacactgca agtttggacc
     1741 gcctatcagg agagtgattt gactagattt gtaagcttat cctattcgag tgaagattgt
     1801 gcagagattg attgtctcgg cgtaaaaaag cttatcaagg tagaaaaatt gggagccaat
     1861 tgcttgctac tttagagagt gaagctcgta aaaaagttgg ttatctgcag gtcaaaacag
     1921 tggcagaagg ttctaataaa gattatgatc gaacaaatga cttttatcga ggtcttggct
     1981 ttaaaaagtt agagattttt cctcaactat ggaatccgca aaatccttgt cagattttga
     2041 ttaaaaagct tgaataatat tacttgacat ctattctcag agtgccatac tgtaagtgta
     2101 atcgccgatt tagctcagtt ggtagagcaa ggcactcgta aagcctaggt tataggtaga
     2161 taaacgactg aggagttgaa aaaatagata ggtagaagat aaccgttaag ccttattctt
     2221 agcggttatt tatattgttt aatagcgctg atatttcatc aattatgcct gttttcgtgt
     2281 ttctggtagt tgttcaagtt tattgctact atttttgatg gtatgaatgt gcttataatg
     2341 tatcccggtt aacgaaagtt ttggacttat actctt
//
LOCUS       DAVBHS010000195         2341 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVBHS010000195 DAVBHS010000000
VERSION     DAVBHS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.195"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..1524
                     /gene="glpO"
                     /locus_tag="VQ651_001979"
     CDS             <1..1524
                     /gene="glpO"
                     /locus_tag="VQ651_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HET2754924.1"
                     /translation="PMLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSK
                     DQVLERQPNLKKEGLVGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFD
                     ESGKITGVVARDLLTDQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHL
                     VVDSSKIKVSQPVYFDTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVD
                     YLLGIVNNRFPESNITIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIAT
                     VESYLSKEKTREDVESAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITD
                     YRKMAEGAMERVVDILKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSR
                     GLDSKEAHYLANLYGSNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFL
                     LRRTNHMLFMRDSLDSIVEPILDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            1594..2298
                     /locus_tag="VQ651_001980"
     CDS             1594..2298
                     /locus_tag="VQ651_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET2754925.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
BASE COUNT          635 a          494 c          558 g          654 t
ORIGIN      
        1 ccaatgctct taccagttta cgatgaagat ggagcaacct ttagcctctt ccgtcttaaa
       61 gtagccatgg acttgtacga cctcttggca ggtgttagca acacaccaac tgcgaacaag
      121 gttttgagca aggatcaagt cttggaacgc cagccaaact tgaagaagga aggcttggta
      181 ggaggtggag tgtatcttga cttccgtaac aacgatgcgc gtctcgtgat tgaaaacatc
      241 aaacgtgcca accaagacgg tgccctcatt gccaaccacg tgaaggcaga aggcttcctc
      301 tttgacgaaa gtggcaagat tacaggtgtt gtagctcgtg atctcttgac agaccaagta
      361 tttgaaatca aggcccgtct ggttattaat acaacaggtc cttggagtga taaagtacgt
      421 aatttgtcta ataagggaac gcaattctca caaatgcgcc caactaaggg agttcacttg
      481 gtagtggatt caagcaaaat caaggtttca cagccagttt actttgacac aggtttgggt
      541 gacggtcgta tggtctttgt tctcccacgt gaaaacaaga cttactttgg tacaactgat
      601 acagactaca caggtgattt ggagcatcca aaagtaactc aagaagatgt agattatcta
      661 cttggcattg tcaacaaccg ctttccagaa tccaacatca ccattgatga tatcgaaagc
      721 agctgggcag gtcttcgtcc attgattgca gggaacagtg cctctgacta taatggtgga
      781 aataacggta ccatcagtga tgaaagcttt gacaacttga ttgcgactgt tgaatcttat
      841 ctctccaaag aaaaaacacg tgaagatgtt gagtctgctg tcagcaagct tgaaagtagc
      901 acatctgaga aacatttgga tccatctgca gtttctcgtg ggtctagctt ggaccgtgat
      961 gacaatggtc ttttgactct tgctggcggt aaaatcacag actaccgtaa gatggctgaa
     1021 ggagctatgg agcgcgtggt tgacatcctc aaagcagaat ttgaccgtag ctttaaattg
     1081 atcaattcta aaacttaccc tgtttcaggt ggagaattga acccagcaaa tgtggattca
     1141 gaaatcgaag cctttgcgca acttggagta tcacgtggtt tggatagcaa ggaagctcac
     1201 tatctggcaa atctttacgg ttcaaatgca ccgaaagtct ttgcacttgc tcacagcttg
     1261 gaacaagcgc caggactcag cttggcagat actttgtccc ttcactatgc aatgcgcaac
     1321 gagttggctc ttagcccagt tgacttcctt cttcgtcgta ccaaccatat gctctttatg
     1381 cgtgatagct tggatagcat cgttgagcca attttggatg aaatgggacg attctatgac
     1441 tggacagaag aagaaaaagc aacttaccgt gctgatgtcg aagcagctct cgctaacaac
     1501 gatttagcag aattaaaaaa ttaagaaaaa ataaaagagg tggagggcag cattccttgt
     1561 cgcccgtccc ttctttttaa tggagacaga aagatgatga atgaattatt tggagaattt
     1621 ctagggactt taatcctgat tcttctagga aatggtgttg ttgcaggtgt ggttcttcct
     1681 aaaaccaaga gcaatagctc aggttggatt gtgattacta tgggttgggg gattgcagtt
     1741 gcggttgcag tctttgtatc tggcaagctc agtccagctc atttaaaccc agctgtgacc
     1801 atcggtgtgg ccttaaaagg tggtttgcct tgggcttccg ttttgcctta tatcttagcc
     1861 cagttcgcag gggccatgct gggtcagatt ttggtttggt tgcaattcaa acctcactat
     1921 gaggcagaag aaaatgcagg caatatcctg gcaaccttca gtactggacc agccatcaag
     1981 gatactgtat caaacttgat tagcgaaatc cttggaacct ttgttttggt gttgacaatc
     2041 tttgctttgg gtctttacga ttttcaggca ggtatcggaa cctttgcagt gggaactttg
     2101 attgtcggta tcggtctatc actaggtggg acaacaggtt atgccttgaa cccagctcgt
     2161 gaccttggac ctcgtatcat gcacagcatc ttgccaattc caaacaaggg agacggagac
     2221 tggtcttacg cttggattcc tgttgtaggc cctgttatcg gagcagcctt ggcagtgctt
     2281 gtattctcac ttttctagtt tatactcttc gaaaatcaaa ttcaaaccac gtcagcgtcg
     2341 c
//
LOCUS       DAVBHS010000196         2305 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVBHS010000196 DAVBHS010000000
VERSION     DAVBHS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.196"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(663..2153)
                     /gene="lysS"
                     /locus_tag="VQ651_001981"
     CDS             complement(663..2153)
                     /gene="lysS"
                     /locus_tag="VQ651_001981"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET2754926.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKTIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
BASE COUNT          660 a          461 c          441 g          743 t
ORIGIN      
        1 tctacatgac cttctgcatt gcttgttttg tatgacataa aatgccctcc ttgattaagt
       61 aagcgtttgc tttttactac acaactataa taactctttt ttcaatcatt ttcaagagtt
      121 ttgcttaaaa accatcttat tttttcatac aatatactag ccctactttc cttaaactct
      181 aaactttagt ctatattaag tgaatcgttg agattttttg ctgccaggca tttgaactac
      241 ttttttattt gcggattcta tctactaata ctataaaaaa acaagtgaat aagacaccag
      301 aacatcctct aataggacct atggacttat atcagtccca tatatactgt ttaagcaaga
      361 tactatccta gttgacgcta acttcctatc ccacttatta attttttatt tatagtgaat
      421 ctgaacaaat caattgggga atccaagtca atttctagta ctttttgaac agtttcagcg
      481 tactatcgta aaatgaaata aaacatgcac aaattgctta gggaatttaa accaatttct
      541 aacaatgtct tagaaatcaa agtgtactat ttcaacttca atatactaca cttcattttc
      601 tccttaaatt cagtctcttt ttgaatcaaa aaatcccctg gtaagagcca gaggataaga
      661 atttatttca ttgttgggaa gagcaataca tcacggatag ttgttgtatc agtgaggagc
      721 atgcagagac ggtcgatacc gattcccaaa ccacctgttg gtggcatacc gtattcaaga
      781 gcttcaatgt agtcatagtc gatgcctgtc gcttcatcat caccaagttc tttggcttta
      841 gcttgggctt caaaacggct aagttggtcg attgggtcgt tcaactcagt aaaggcatta
      901 ccgtactcct tagtcatgat aaagagctcg aaacggtcag taaagcgttg gtcttcagga
      961 tttttcttag cgagtggaga tacagctact ggatgtccat agacaaaggt tggttggatt
     1021 aaagtttctt caacaaactc ttcaaagaag gcattgatga tgtgaccaac ctcagtgtag
     1081 tgtttctcaa ctggaacttt cttctcagca gcgatagttt tagcttcttc caaagtcatg
     1141 tcttgccaga aatcgacacc agtaatttct ctgatagcat ccaccatatg aacacgctta
     1201 aatggttcgt taatcttgat ttcagtacct tggtagttga ctgggccatc acctttgact
     1261 gatttagcag cgtgttggat aatgccttca gtcaagtcca tgatgtcttg gaagtctgca
     1321 taagcttggt aaacttcgat agaagtgaac tcagggttat gagtagcgtc cattccttcg
     1381 ttacggaaga tacggccaat ttcatagaca cgttccatac cacccacgat aaggcgtttc
     1441 aagtgaagct cagtcgcgat acgaagcacc atgtcaatgt tttgggcatt gtggtgggtg
     1501 ataaatggac gggcagcagc accaccggct tcattatgaa gaacaggtgt ttccacttca
     1561 aggaatcctt tttggtcaag gtaacgacgg atttcagaga tgatttttga acgagtgaca
     1621 aagcgttcaa agctttcacg attagaaatc aagtcaaggt aacgtttacg gtaaattgtt
     1681 tcaacgtctg tcaaaccatg gaatttctca ggaagaggac gaagagcctt agacaagtgt
     1741 gtgatgtggg ttgccttgat agagagttct cccatatccg tacgcatcac ttcaccttcg
     1801 acaccaagga agtcaccaag gtctgctttt ttgaagattt cgtagttttc ttcaccgaca
     1861 gcatccttac gaacgtagat ctgaatctgg ccttcgcggt cttgaaggtg ggcaaaacca
     1921 acttttcctt taccacgttt ggttatcaag cgtcctgcga tagtagctgt ttcgttttta
     1981 tcgtgtaatt gttctttatc gaggttggca tatttatctt ttaattcttg tgaatttgca
     2041 gtacgttcaa aacgttttcc gaaaggatcg attccttgtt cgcggagcgc agccattttt
     2101 tcacggcgaa cgatctgctg gtcatttagt tcttccatat gttctgtaga catgggatcc
     2161 tcctagtttt actcaaaatt gaatacgatg agtcattttt tgcgatactc ccattttatc
     2221 ataaataagc aagaaattca cggataatct ttaaaaacta aaaagaacct gacgctaaaa
     2281 tcactggaaa gaagtgaaag tatct
//
LOCUS       DAVBHS010000197         2213 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVBHS010000197 DAVBHS010000000
VERSION     DAVBHS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.197"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            101..1078
                     /locus_tag="VQ651_001982"
     CDS             101..1078
                     /locus_tag="VQ651_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2754927.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGVRFEAEK"
     gene            1075..2157
                     /locus_tag="VQ651_001983"
     CDS             1075..2157
                     /locus_tag="VQ651_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2754928.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFELLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
BASE COUNT          696 a          497 c          404 g          616 t
ORIGIN      
        1 aaagctaata gcttatttga aattttctcc tctctgttgg atagtcccgt ctatctatgt
       61 tataatgaaa gaaggattta aaatcggaaa aggagtattt atgctaaaat taggtgtcat
      121 cggaactggc gctatcagcc atcatttcat agaagcagcc cataccagtg gagaatacca
      181 actggtcgca atctattcta gaaaactaga aactgcagca acctttgctt ctcgctatca
      241 gaatatccaa ctattcgatc aattagaagt cttcttcaaa tcttcttttg atttagtcta
      301 tatcgccagt ccaaactcct tacattttgc tcaggcaaaa gctgccttgt ctgctggtaa
      361 acatgttatt cttgaaaagc cagctgtcac tcaaccacaa gaatggtttg atttgattca
      421 aacagctgaa aaaaataact gttttatctt tgaagcagct cgtaattatc acgagaaagc
      481 ctttactact atcaaaaact ttttagcaga taagcaagtg ttaggagcag atttcaacta
      541 tgccaaatac tcttccaaga tgcctgactt attggctgga caaacaccaa atgtcttttc
      601 agaccgtttt gctggtggag ccctcatgga tttggggatt tatcccctct atgctgctgt
      661 tcgtcttttt gaaaaagcta atgacgcgac ttaccatgct caacagcttg acaatagcat
      721 tgacctaaat ggagatggta tcctcttcta cccagactat caagttcaca tcaaggctgg
      781 aaaaaacatc acttccaatc ttccttgcga gatttataca acagatggaa ccttgactct
      841 caacacgatt gagcatattc gctcagctat ttttaccgac caccaaggaa atcaagtcca
      901 gcttcctatc caacaggctc ctcatacgat gactgaggaa gtcgctgcat ttgcacacat
      961 gatccagcaa ccagatctga atctctacca aacttggctg tatgatgcag gctctgttca
     1021 tgagctacta tataccatgc gccagactgc tggcgttaga tttgaggcag aaaaatgaaa
     1081 accaaactac cgactgaatg gcaagaactg agtgaccaac tcggtttcca agaattcacc
     1141 cccattcaaa ctcaactatt tgagctcctt cttgctggag aaaacctcct aggagtgagc
     1201 caaaccggaa ctggtaagac cctagcgtat ctcctaccaa gtcttctcag actacaaaag
     1261 aaaaaagccc aacaactctt gattctagca ccaaacacgg aacttgctgg acagattttt
     1321 gatgtatgta aaacgtgggc agaagctatc ggattgactg ctcaactctt cctttcaggt
     1381 tcaagtcaga aacgacaaat tgaacgtctt aaaaaggggc cagaaattct gattggaact
     1441 cctggccgta tctttgaact cattaaactt aaaaaaatca agatgatgaa tgtggaaacc
     1501 atcatcctgg atgaatttga ccaattacta gatgattctc agattcactt tgtagagaaa
     1561 atcactcact acgcacctcg tgaccaccaa ctggtctaca tgagtgccac gaccaagttt
     1621 gaccaagaaa agattgtgcc gaatacacgc actattgatc tctctgatca aaaattggac
     1681 aatatccagc atttctacat gcaggtagac caacgtcatc gagtggatat gctacgaaaa
     1741 ctggctcatg tagaggattt ccgtggtcta gtcttcttca atagcctgtc agaccttggg
     1801 aatgccgagg aaaaactaca gtatcgtgat atattggctg tttccctcgc tagtgatgtt
     1861 aatgttaaat ttagaaaaat catcttagaa aagtttaaag ataaccaact aaccctgctt
     1921 cttgcaactg accttttggc tcgtggaatt gatatcgata gcctagaatg tgtcgtaaac
     1981 tttgatattc ctagagatag cgaaacctac acacaccgtg ctggccgtac tggtcgtatg
     2041 ggcaaggaag gttatgttat cactctcgtc actcatccag aagaaatcaa aaaacttaaa
     2101 aagtttgcaa gtatccgaga aattgtccta aaaaaccaag aactctatat caaataaagc
     2161 cccattgctc tatacacatc cagtgtatgg agctttttta taagcaattc aat
//
LOCUS       DAVBHS010000198         2122 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVBHS010000198 DAVBHS010000000
VERSION     DAVBHS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.198"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            75..524
                     /locus_tag="VQ651_001984"
     CDS             75..524
                     /locus_tag="VQ651_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2754929.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDAFVTNFVYEKEGQSRKKSMSYESVLPVRQIFD"
     gene            681..1091
                     /locus_tag="VQ651_001985"
     CDS             681..1091
                     /locus_tag="VQ651_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HET2754930.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKRIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            1207..>2122
                     /gene="pavB"
                     /locus_tag="VQ651_001986"
     CDS             1207..>2122
                     /gene="pavB"
                     /locus_tag="VQ651_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HET2754931.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAV"
BASE COUNT          649 a          406 c          471 g          596 t
ORIGIN      
        1 aaggcgacgt tgacgcggtt tgaagagatt ttcgaagagt ataagaaaaa atcagtcccc
       61 taaaggagca gattatgaag ttattgtcta tcgccatttc tagctataat gcagcagcct
      121 atcttcatta ctgtgtggag tcgctagtga ttggtggtga gcaagttggg attttgatta
      181 tcaatgacgg gtctcaggat cagactcagg aaatcgctga gtgtttagct agcaagtatc
      241 ctaatatcgt tagagccatc tatcaggaaa ataaatgcca tggcggtgcg gtcaatcgtg
      301 gcttggcaga ggcttctggg cgctatttta aagtagttga cagtgatgac tgggtggatc
      361 ctcgtgccta cttgaaaatt cttgaaacct tgcaggaact tgagagcaag ggtcaagagg
      421 tggatgcctt tgtgaccaat tttgtctatg aaaaggaagg tcagtctcgt aagaagagta
      481 tgagttacga gtcagtcttg cctgttcggc agatttttga ctaggaccag gtcggaaatt
      541 tctccaaagg ccagtatatc atgatacact cgctgattta tcggacagat ttgttgcgtg
      601 ctagccagtt ctaactgcct gaacatactt tttatgtcga tgatctcttt gtctttacgc
      661 cccttcagca ggtcaagacc atgtactatc tgcctgtcga tttctatcgt tatttgattg
      721 ggcgtgagga ccagtctgtc aatgagcaag tgatgattaa gcgcattgac cagcaactca
      781 aggtcaatcg actcttggta gaccagcttg atttgtccca agtgagtcat cctaaaatgc
      841 gagaatatct gctgaatcat attgaaatca cgacggtgat ttccagtacc ctgctcaacc
      901 gatctgaaac agcggagcat ctggcaaaaa aacgccaatt gtggacctat attcagcaga
      961 aaaatccaga agtctttcag gctattcgta agaccatgtt gagccgtttg accaaacatt
     1021 ctgtcttgcc agatcgcaaa ctgtccaatg tcgtctatca aatcaccaaa tctgtttatg
     1081 gatttaatta atataagtgt tttataagag ggatttaaga aaaattttaa ctttttctta
     1141 gtccttttta atttcaggag attatactag agtcatcaaa taaagaaaga ctctaaggag
     1201 aatcctatga aattcaatcc aaatcaaaga tatactcgtt ggtctattcg ccgtctcagt
     1261 gtcggtgttg cctcagttgt tgtggctagt ggcttctttg tcctagttgg tcagccaagt
     1321 tctgtacgtg ccgatgtggt caatccgacc ccaggtcaag tcttacctga agagacatcg
     1381 ggaacgaaag agggtgactt atcagaaaaa ccaggagaca ccgttctcac tcaagcgaaa
     1441 cctgagggcg ttactggaaa tacgaattca cttccgacac ctacagaaag aactgaagtg
     1501 agcgaggaaa caaactcttc tagtctggat acactttttg aaaaagatga agaagctcaa
     1561 aaaaatccag agctaacaga tgtcttaaaa gaaactgtag atacagctga tgtggatggg
     1621 acacaagcaa gtccagcaga aactactcct gaacaagtaa aaggtggagt gaaagaaaat
     1681 acaaaagaca gcatcgatgt tcctgctgct tatcttgaaa aagctgaagg gaaaggtcct
     1741 ttcactgccg gtgtaaacca agtaattcct tatgaactat tcgctggtga tggtatgtta
     1801 actcgtctat tactaaaagc ttcggataat gctccttggt ctgacaatgg tactgctaaa
     1861 aatcctgctt tacctcctct tgaaggatta acaaaaggga aatacttcta tgaagtagac
     1921 ttaaatggca atactgttgg taaacaaggt caagctttaa ttgatcaact tcgcgctaat
     1981 ggtactcaaa cttataaagc tactgttaaa gtttacggaa ataaagacgg taaagctgac
     2041 ttgactaatc tagttgctac taaaaatgta gacatcaaca tcaatggatt agttgctaaa
     2101 gaaactgtcg aaaaagctgt ta
//
LOCUS       DAVBHS010000199         2089 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVBHS010000199 DAVBHS010000000
VERSION     DAVBHS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.199"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(374..1981)
                     /locus_tag="VQ651_001987"
     CDS             complement(374..1981)
                     /locus_tag="VQ651_001987"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET2754932.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
BASE COUNT          599 a          475 c          393 g          622 t
ORIGIN      
        1 tttcatttta ctatagtgga ttaaattaaa aaagtataat gtattgaatc tataacagta
       61 catcttgact gctaaaatat ttctataaat taatttgact tttctgatag agatgttcac
      121 atcttatttc aattcactat acaatccagt tcctaaaact ttgctagaaa ttgatttggc
      181 tttccgaatt aatttgttca aattttattt catttcaata tactgataaa tccattcact
      241 agcacagggc aagagggagt cgctattgat ttcaacggta gcaggtacat acagaggccc
      301 cctacctcag atatattgca atatcatcgt atgcaaaaaa cctctgagat ttttctcaaa
      361 ggttctgatt ttgctaattg ctgttctcaa ccgctgcagt aacaaaggca gtgtagagtt
      421 cttctggtcg gtttggacgg cttgacagtt cagggtgata ctgacaagct acaaagaatt
      481 tattttcagg aatttccacg atttctacca aacgattgtc tggagaaact cctgaaaaga
      541 caaaacctgc tgcctcaaac tgctcacgga aggcattatt aaactcataa cggtgacggt
      601 gacggcgttg caccacttct tgattgtgat aagcagcagc agccttagag ccacgtttca
      661 acttagacgg ataaagtccc aaacgaaggg ttccacccat atcctcaata tcaatctgat
      721 cacgcatgat atcaatgata gggtattttg tttctggtgc aagctctgca gaattggcac
      781 cttcaagacc taaaacgtga cgagcaaact cgatacatgt caactgcatt cccaagcaga
      841 ctcccaacat tggaacatca ttttcacgcg catagcggat ggcttggatt ttcccttctg
      901 taccacgttg accaaaacca cctggtacga tgatcccgtc cgcatcagac aagagttctg
      961 ctacattctc tgctgtcaca tcattggcat tgacccaatt gattttaact tctacatcat
     1021 tgacatagcc agagtgtttc aaggcttcga ccactgagat ataggcatct tgcaactcca
     1081 catacttacc aacaagggaa atcttaactt gtttcttgag gttcatgacc ttgtccacca
     1141 tggctgacca ttctgtcata tccgctgctg gtgcgtctaa tttcaaatga tcacaaacaa
     1201 tttggtccat cccttgtgcc tgcaagttca gtggaatttg gtaaaggtgt tcaacatcca
     1261 acgattcgat aacggcttct ggtgccacat cacagaactg ggccagttta tttttaattc
     1321 cttgaccagc tggctcttct gtacgaataa ccaacatatt tggttggatt cccaagccac
     1381 gcaattcttt gacagagtgt tgggttggtt tggttttcat ttcaccagca gccttgaggt
     1441 aaggaagcaa ggttgtatgg atatacatga cattatccgc acccacatct gccttcatct
     1501 gacgaagagc ctctaggaat ggcaaggact cgatatctcc tactgttcca ccaacctctg
     1561 tgataatgac atcagagtcg gtcgttagag cggcacgctt gattttttct ttcaaagcat
     1621 ctgtgatatg aggaatgact tgaacagttg ccccaaggta ttctccacgg cgttctttac
     1681 gaagaacttc actgtaaatt ttcccagttg tcacgttgga atatttgttg agattgatat
     1741 cgatgaaacg ttcatagtga cccaagtcca aatctgtctc agctccgtca tctgtcacaa
     1801 aaacttcccc gtgctggtaa ggactcatgg ttcccggatc aatattgata taagggtcaa
     1861 acttttgaat ggttactttg agaccacgat ttttcaagag acggcctaga ctcgctgcca
     1921 caatcccttt cccaatagac gataccacac caccagttac aaaaatatat ttcgtagaca
     1981 tagattcctc tttctaaaat gctcaaggtc ttgttaacta cgaggggata tagaaaacaa
     2041 gaccctacta ataagaataa tctgggacga ctgcaccaga ttcacaact
//
LOCUS       DAVBHS010000200         2062 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVBHS010000200 DAVBHS010000000
VERSION     DAVBHS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.200"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(37..219)
                     /locus_tag="VQ651_001988"
     CDS             complement(37..219)
                     /locus_tag="VQ651_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET2754933.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            576..>2062
                     /gene="pcpA"
                     /locus_tag="VQ651_001989"
     CDS             576..>2062
                     /gene="pcpA"
                     /locus_tag="VQ651_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HET2754934.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDGA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLWYYLNESGSKATGWVKDKGLWYYL"
BASE COUNT          745 a          284 c          334 g          699 t
ORIGIN      
        1 cgcttaaatc gtgcatcggt tagttgttta cttgcttcat aatttcgcag ggagtctatt
       61 gactctttgg taggtgtcaa tgtttttttc atctatcccg agaattattt tcccgccatt
      121 tgtatttgca aatgctgagt aggtttccca gaaagactct ggaagattgt ttttagcttt
      181 tttgtattct aaatcaaccc cttcaaattt taagtccata tttttccttt acatctgttt
      241 tttgtggttc tggtatttgt tcaagttgag tgataatata gcgaattgaa tttcgagagt
      301 ttttactcag ttaatttctt ttttaaccca ctttaatttc ttttttaacc cactttaatt
      361 tcttttttaa cacgggttaa aaaagaaatt aaagtgggtt aatttttctt gacttaaaat
      421 ttaaaaatga tatactgtgt ttatggagag atgacaattt ataaaatgta ctactctatt
      481 ctactagata atgattgaat aaaacttttt ataacaacgg ctagcaaagt tataatttta
      541 tatctattag aaaataaata tgtaaggaaa tttaaatgaa aaaaactaca atattatcat
      601 taactacagc tgcggttatt ttagcagcat atgtccctaa tgaaccaatc ctagcagata
      661 ctcctagttc ggaagtaatc aaagagacta aagttggaag tattattcaa caaaataata
      721 tcaaatataa ggttctaact gtagaaggta acataggaac tgttcaagtg ggtaatggag
      781 ttactcctgt agagtttgaa gctggtcaag atggaaaacc attcacgatt cctacaaaaa
      841 tcacagtagg tgataaagta tttaccgtta ctgaagtagc tagtcaagct tttagttatt
      901 atccagatga aacaggtaga attgtctact atcctagctc tattactatc ccatcaagca
      961 taaaaaaaat acaaaaaaaa ggcttccatg gaagtaaagc taaaactatt atttttgaca
     1021 aaggcagtca gctggagaaa attgaagatg gagcttttga tttttctgaa ttagaagaga
     1081 ttgaattgcc tgcatctcta gaatatattg gaacaagtgc attttctttt agtcaaaaat
     1141 tgaaaaagct aaccttttcc tcaagttcaa aattagaatt aatatcacat gaggcttttg
     1201 ctaatttatc aaatttagag aaactaacat taccaaaatc ggttaaaaca ttaggaagta
     1261 atctatttag actcactact agcttaaaac atgttgatgt tgaagaagga aatgaatcgt
     1321 ttgcctcagt tgatggtgtt ttgttttcaa aagataaaac ccaattaatt tattatccaa
     1381 gtcaaaaaaa tgacgaaagt tataaaacgc ctaaggagac aaaagaactt gcatcatatt
     1441 cgtttaataa aaattcttac ttgaaaaaac tcgaattgaa tgaaggttta gaaaaaatcg
     1501 gtacttttgc atttgcagat gcgattaaac ttgaagaaat tagcttacca aatagtttag
     1561 aaactattga acgtttagcc ttttacggta atttagaatt aaaagaactt atattaccag
     1621 ataatgttaa aaattttggt aaacacgtta tgaacggttt accaaaatta aaaagtttaa
     1681 caattggtaa taatatcaac tcattgccgt ccttcttcct aagtggcgtc ttagattcat
     1741 taaaggaaat tcatattaag aataaaagta cagagttttc tgtgaaaaaa gatacatttg
     1801 caattcctga aactgttaag ttctatgtaa catcagaaca tataaaagat gttcttaaat
     1861 caaatttatc tactagtaat gatatcattg ttgaaaaagt agataatata aaacaagaaa
     1921 ctgatgtagc taaacctaaa aagaattcta atcagggagt agttggttgg gttaaagaca
     1981 aaggtttatg gtattactta aatgaatcag gttcaaaggc tactggttgg gttaaagaca
     2041 aaggcttatg gtattactta aa
//
LOCUS       DAVBHS010000201         2020 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVBHS010000201 DAVBHS010000000
VERSION     DAVBHS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.201"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            73..822
                     /gene="truA"
                     /locus_tag="VQ651_001990"
     CDS             73..822
                     /gene="truA"
                     /locus_tag="VQ651_001990"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET2754935.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQAI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFTVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            812..1585
                     /locus_tag="VQ651_001991"
     CDS             812..1585
                     /locus_tag="VQ651_001991"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET2754936.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            1563..>2020
                     /locus_tag="VQ651_001992"
     CDS             1563..>2020
                     /locus_tag="VQ651_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2754937.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKI"
BASE COUNT          554 a          394 c          469 g          603 t
ORIGIN      
        1 agatttttat acccatacaa tatttgtttt aggtccttgt ttacagacta atttcttgat
       61 aaaatagagg ttatgacgag atataaagca actatttcct atgatggtta tgcctttgct
      121 ggctttcagc gccagcctca tgcgcgtagc gttcaggaag aaattgaaaa aaccttgacc
      181 agattaaata aagggcaagc cattactgtt cacggtgctg gtaggacaga tagtggggtt
      241 catgccctgg gacaggtcat tcattttgac ctgccttatc agatggatga ggagaaactc
      301 cgttttgcct tggataccca gtctcctgaa gatattgatg tgatttcgat tgagcttgtg
      361 gcagatgatt ttcattgccg ttatgccaag catagcaaga cctatgagtt tactgtggat
      421 agaggacgtc ccaaaaatcc gatgcgccgt cactatgcca cccactttcc ctacccactc
      481 gatgtggaac gaatgcagat tgcaatcaaa aagctagagg gaacccatga ttttaccggt
      541 tttacagcct ctgggactag tgtagaggat aaggttcgca ccatcacaga agctagttta
      601 atagtcgatg agacaggaca atttttgacc tttacctttt caggaaatgg tttcttgtat
      661 aaacagattc gcaatatggt ggggactctt ctcaaaatcg gtaacaaccg catgccagta
      721 gagcagattg atctcatctt ggagaagaag gacaggcaac ttgcaggtcc cactgcagca
      781 ccaaatggtt tgtatttaaa ggagattcgt tatgaagaat aatcgtattt tagcactttc
      841 tggaaatgat atttttagtg gtggtggact gtcagctgat ttggctacct ataccttgaa
      901 cggcttgcat gggtttgtag cagtgacttg tttgacagcc ttgacagaaa aaggatttga
      961 agtctttcca actgatgata ccatttttca acatgaatta gatagcttgc gtgatgtgga
     1021 atttggggga attaagattg gtcttctccc tactgtcagt gtggctgaga aggccttgga
     1081 ctttatcaaa caacgcccag gagtacctgt ggtgttggat cctgtcttgg tctgcaagga
     1141 aacgcatgat gtagctgtca gtgagctctg ccaagagttg attcgctttt tcccttatgt
     1201 cagtgtgatt acgcctaatc tcccagaagc agaattatta tccggtcagg aaattaaaac
     1261 cttggaagac atgaaaactg cagcgcagaa attgcatgat ttaggagcgc cagcagtcat
     1321 tatcaaggga ggcaatcgtc ttagtcagga caaggctgtg gatgtctttt atgatggaca
     1381 gacctttact atcctagaaa atccagttat ccaaggccaa aatgctggtg caggttgtac
     1441 ctttgcctct agcattgcca gtcacttgat taaaggtgat aaacttttgc cagcagtaga
     1501 aagctctaag gctttcgttt atcgtgctat tgcacaagca gatcagtatg gagtaagaca
     1561 atatgaagca aacaaaaaca actaaaatcg cccttgtatc cctattaacc gccctttctg
     1621 tggttctagg ttatttctta aaaatcccaa cacctacagg aattctaact cttttagatg
     1681 ctggtgtctt ctttgcggcc ttttactttg gtagtcgtga aggagcggta gtcggaggac
     1741 tagcaagttt cttgattgac ctcttatcag gctaccctca gtggatgttc tttagcttgg
     1801 tcaaccatgg cttgcaggga tttttcgcag gatttaaagg aaaaagtcag tggttaggcc
     1861 ttattttagc aactattgcc atggtaggag gctacgcctt gggttctact ttgatgaatg
     1921 gctgggcagc agccctccca gaaattctac caaatttcat gcaaaatatg gtagggatga
     1981 ttgtaggatt tattcttagt caaagtatca agaagattaa
//
LOCUS       DAVBHS010000202         1935 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVBHS010000202 DAVBHS010000000
VERSION     DAVBHS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.202"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            88..393
                     /locus_tag="VQ651_001993"
     CDS             88..393
                     /locus_tag="VQ651_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2754938.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            441..1916
                     /locus_tag="VQ651_001994"
     CDS             441..1916
                     /locus_tag="VQ651_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HET2754939.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNIVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
BASE COUNT          551 a          392 c          386 g          606 t
ORIGIN      
        1 tttccgaacc agaaaaactt tatgcttaca ttgctgaagc aaccgcttaa tcttgtctat
       61 taaaaaataa aatcggagga aatccaaatg attaaaattc ttgctgcctg cggtgcaggt
      121 gttaactcaa gtcaccaaat taaaagtgct ctagaagaag aactttcaaa ccgtggttat
      181 gatgttcact gtgatgcagt catggtgaaa gatgtaaacg aagaccttat gaaaggttac
      241 gatatcttta caccaatcgc tgcaacagac cttggttttg aaccaggtat cccagttatc
      301 gaagctgggc caatcttatt ccgtatccca gcaatgagcg ctccagtatt tgacaatatt
      361 gaagcagcta ttaaagaaca cggattaagc taattctaat ttgttaacat tcatataaca
      421 taaaaaaggg aggaactgtt atggatgcaa tctttgacct aatcggaaag gttttcaatc
      481 ccattttaga aatgggtgga cctgtcatca tgttaatcat tttgacagta ttggctttac
      541 tttttggagt gaaattctcc aaagcgcttg aaggtggtat caaacttgcc atcgctctta
      601 caggtatcgg tgctatcatc ggtatgctaa atggtgcttt ctcagcatca cttgcaaaat
      661 tcgttgaaaa cactggtatc caattgagta ttaccgacgt tggttgggca ccactcgcaa
      721 caatcacttg gggttctgct tggacactat acttcttgct catcatgttg attgtcaaca
      781 tagtgatgct agctatgaag aaaacagata cacttgacgt tgatatcttt gatatctggc
      841 acttgtctat cacaggtctc ttgattaaat ggtatgctga taacaatggt gtgagtcaag
      901 gggtttcact ctttattgct acagcagcta tcgtccttgt cggtgtgttg aaaattatca
      961 actctgactt gatgaaacct acatttgatg accttcttaa cgccccaagt tcatcaccaa
     1021 tgacatcaac tcacatgaac tacatgatga acccagttat catggttttg gataagattt
     1081 ttgaaaaatt cttcccaggc cttgataaat atgactttga tgctgctaaa ttgaacaaga
     1141 aaatcggttt ctggggatct aaattcttca tcggtttcat ccttggtatc gttatcggta
     1201 ttatgggaac tccacatcca attgcaggtg ttgcagatgc agataaatgg cgtcttgtta
     1261 tcaaaggatg gttgtctctt ggtttgactg ccggtgtatc tttggaactc ttctcactta
     1321 tcggttcatg gttcatcgca gccgtagaac cactatcaca aggtattaca aacgttgcta
     1381 ctaaacgtct tcaaggacgt aaattcaata tcggtcttga ctggccattc atcgctggtc
     1441 gtgctgaaat ctgggcttgt gccaacgtac ttgcaccaat catgttgatt gaagcagtgc
     1501 ttctttcaaa agttggaaat ggtatcttgc cacttgcagg tatcatcgct atgggtgtta
     1561 ctccagctct cttggttgta actcgtggta aattgctccg tatgattatc ttcggaacac
     1621 tcttgttgcc actcttcctt ctttcaggta cacttattgc accatttgca acagaacttg
     1681 ctaaaggtgt aggtgccttc ccagaaggtg tgagccaaac tcaattgatt actcactcta
     1741 ctcttgaagg accaatcgaa aaacttcttg gttggacaat tggtaacact acaactggtg
     1801 atatcaaagc aatccttggt gcagtagtct tccttgtatt ctatatcggt atctttgctt
     1861 ggtacagaaa acaaatgatc aaacgtaacg aagagtacgc agcaaaagca aaataatgcg
     1921 ctcctataaa atata
//
LOCUS       DAVBHS010000203         1856 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVBHS010000203 DAVBHS010000000
VERSION     DAVBHS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.203"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..1601
                     /gene="phtA"
                     /locus_tag="VQ651_001995"
     CDS             <1..1601
                     /gene="phtA"
                     /locus_tag="VQ651_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HET2754940.1"
                     /translation="NDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPH
                     GDHYHFIPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPN
                     LKIDSNSSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHT
                     LTAKKENVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKE
                     KLVDDLLAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISD
                     EGDAYVTPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAA
                     IYNRVKGEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGY
                     TLEDLFATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETP
                     AEPEVPQVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIM
                     AEAEKLLALLKGSNPSSVSKEKIN"
     gene            1759..>1856
                     /locus_tag="VQ651_001996"
     CDS             1759..>1856
                     /locus_tag="VQ651_001996"
                     /inference="COORDINATES: protein motif:HMM:TIGR01363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2754941.1"
                     /translation="MKINKKYLAGSVATLVLSVCAYELGLHQAQTV"
BASE COUNT          656 a          337 c          361 g          502 t
ORIGIN      
        1 gtaatgacat tgatagtctc ttgaaacagc tctacaaact gcctttgagt caacgacatg
       61 tagaatctga tggccttgtt ttcgacccag cgcaaatcac aagtcgaaca gctagaggtg
      121 ttgcagtgcc acacggagat cattaccact tcatccctta ctctcaaatg tctgaattgg
      181 aagaacgaat cgctcgtatt attccccttc gttatcgttc aaaccattgg gtaccagatt
      241 caaggccaga acaaccaagt ccacaaccga ctccggaacc tagtccaggc ccgcaacctg
      301 caccaaatct taaaatagac tcaaattctt ctttggttag tcagctggta cgaaaagttg
      361 gggaaggata tgtattcgaa gaaaagggca tctctcgtta tgtctttgcg aaagatttac
      421 catctgaaac tgttaaaaat cttgaaagta agttatcaaa acaagagagt gtttcacaca
      481 ctttaactgc taaaaaagaa aatgttgctc ctcgtgacca agaattttat gataaagcat
      541 ataatctgtt aactgaggct cataaagcct tgtttgaaaa taagggtcgt aattctgatt
      601 tccaagcctt agacaaatta ttagaacgct tgaatgatga atcgactaat aaagaaaaat
      661 tggtagatga tttattggca ttcctagcac caattaccca tccagagcga cttggcaagc
      721 caaattctca aattgagtat actgaagacg aagttcgtat tgctcaatta gctgataagt
      781 atacaacgtc agatggttac atttttgatg aacatgatat aatcagtgat gaaggagatg
      841 catatgtaac gcctcatatg ggccatagtc actggattgg aaaagatagc ctttctgata
      901 aggaaaaagt tgcagctcaa gcctatacta aagaaaaagg tatcctacct ccatctccag
      961 acgcagatgt taaagcaaat ccaactggag atagtgcagc agctatttac aatcgtgtga
     1021 aaggggaaaa acgaattcca ctcgttcgac ttccatatat ggttgagcat acagttgagg
     1081 ttaaaaacgg taatttgatt attcctcata aggatcatta ccataatatt aaatttgctt
     1141 ggtttgatga tcacacatac aaagctccaa atggctatac cttggaagat ttgtttgcga
     1201 cgattaagta ctacgtagaa caccctgacg aacgtccaca ttctaatgat ggatggggca
     1261 atgccagtga gcatgtgtta ggcaagaaag accacagtga agatccaaat aagaacttca
     1321 aagcggatga agagccagta gaggaaacac ctgctgagcc agaagtccct caagtagaga
     1381 ctgaaaaagt agaagcccaa ctcaaagaag cagaagtttt gcttgcgaaa gtaacggatt
     1441 ctagtctgaa agccaatgca acagaaactc tagctggttt acgaaataat ttgactcttc
     1501 aaattatgga taacaatagt atcatggcag aagcagaaaa attacttgcg ttgttaaaag
     1561 gaagtaatcc ttcatctgta agtaaggaaa aaataaacta atgaaaaatg aaagtctcga
     1621 taaagaggct ttcattttta ttatgtatat atgtaaaatt cttgacaagc aatattaaaa
     1681 agagtaaact attaactagt taattaaccg gtttattact ttatagtgaa tcaaatatac
     1741 ttaagaaaag aggaaagaat gaaaatcaat aaaaaatatc tagctgggtc agtagctaca
     1801 cttgttttaa gtgtctgtgc ttatgaacta ggtttgcatc aagctcaaac tgtaaa
//
LOCUS       DAVBHS010000204         1775 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVBHS010000204 DAVBHS010000000
VERSION     DAVBHS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.204"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..317)
                     /locus_tag="VQ651_001997"
     CDS             complement(<1..317)
                     /locus_tag="VQ651_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754942.1"
                     /translation="MILSKVRCYIYNSKIRGKVLYELYEFINENKQLMDAINSIKVDS
                     SNGFSIQILHLGEQQTTVLLTDFFTKTLDLFKEFRVTNKSTLEQVKNQISILNSSGPK
                     MDLT"
     gene            complement(330..1349)
                     /locus_tag="VQ651_001998"
     CDS             complement(330..1349)
                     /locus_tag="VQ651_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HET2754943.1"
                     /translation="MSNTLKQLLLFREEFIKNILNGIDVDETVVIENDFVEELENFLK
                     ENDLLDFSNSIFFQLSGDKVIVNNIYPGYLAYFNRFLTFYPKIFAIKEVGEYIDFLNF
                     EKRIADMFYCWGFNANSRVLTAEKIIEIPNHQINAKYIKKVIKMSELLFGVDSNNRIR
                     LLNNRRELIKTIFQGSVIKSAIPALAATLDTISNSGSFQISLSKQIINNLVLSGKDKV
                     TIPRIELDNVVLSRQKIIVSVSYINDFLDLRFPDKIEYILNFLEGSGFNTKVMVYFGK
                     IVEEDKNYFVNLEKPQYFDFTSILFNKLFFKELNKFDYMVIEELIPNLDEDVMMTEYI
                     KEYTE"
BASE COUNT          614 a          302 c          181 g          678 t
ORIGIN      
        1 gttaaatcca tttttggacc agaagaattt agtatgctaa tttgattttt cacctgctca
       61 agagtactct tatttgtaac cctaaattcc ttaaacaagt ctaaagtttt agtaaaaaaa
      121 tctgttaata agacagttgt ttgctgttct cctaaatgta atatttgaat actaaaacca
      181 ttactactgt caactttaat tgaattaatt gcgtccatca attgcttatt ttcatttata
      241 aattcatata attcatacag aactttacct ctaattttag aattatatat gtaacaacgt
      301 actttactta aaatcataat agattgctcc tattcagtat actcttttat atactctgtc
      361 atcattacat cttcatcaag atttggaatg agttcttcaa taaccatgta atcaaacttg
      421 tttaattctt taaagaataa cttattgaaa agtatactgg taaaatcaaa atactgaggt
      481 ttttcaagat taacaaaata gttcttatct tcttctacaa ttttcccaaa gtagaccatt
      541 actttagtat tgaaaccaga tccttctaaa aaatttaaaa tgtattctat tttatctgga
      601 aatctcaaat ccaaaaaatc atttatatac gaaactgata caataatctt ctgtcttgaa
      661 agaacaacat tatctaactc tatcctagga attgtaactt tatctttacc acttaatact
      721 aaattattaa ttatctgctt tgataaagaa atttgaaatg atccactgtt actgattgta
      781 tccaaagtag cagctagagc aggaattgct gacttaatta cagaaccttg gaaaatagtt
      841 tttatcaatt cccttctatt gttaagcaac cgaattctgt tattactatc aacaccaaac
      901 aagagttcac tcatttttat aacttttttt atgtacttag cattgatttg gtgatttggg
      961 atttcaatta ttttttcagc tgtcaacact ctagaatttg cattaaatcc ccaacaataa
     1021 aacatatccg caattctctt ttcaaaattt aaaaagtcaa tatattctcc aacttcttta
     1081 atagcgaata ttttcggata aaatgtaagg aacctattaa aatatgctaa atatcctgga
     1141 taaatattat tcactattac tttatctcca gataactgaa aaaatatgct atttgaaaaa
     1201 tctagcaaat cattctcttt taaaaagttc tccaactctt ctacaaaatc attttctatt
     1261 actactgttt catccacatc aatcccattc aaaatatttt ttatgaactc ctctctaaaa
     1321 agtagtaatt gcttcaatgt atttgacata tattcatctg gataatatgt ttccaaatta
     1381 acattagcga gtctaggagc ttctttgatg attatgttaa ctatatcctg aactatttct
     1441 tctgatgtcc ctaccttctg gtgttctctc ctgtgtttat tataataaca ttgtccgaat
     1501 agtagctgaa aaaacaaatt actatcaaat aatctcgtta gaattaatag ctttcttatt
     1561 gacttattat caacttttaa actttctttt tcaatttgcc tcaactctac aatgtcttga
     1621 taaataaaat tatgttctat aaaaatttac ttcaactagc tatggtttaa tagatttttt
     1681 aaaggggata attgtctaaa atttaacttt ttacaaatca agatgtctct ataaactagc
     1741 aacctctcta ataactttac ccctatagct ataga
//
LOCUS       DAVBHS010000205         1716 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVBHS010000205 DAVBHS010000000
VERSION     DAVBHS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.205"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            720..1712
                     /locus_tag="VQ651_001999"
     CDS             720..1712
                     /locus_tag="VQ651_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2754944.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYDGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
BASE COUNT          530 a          270 c          387 g          529 t
ORIGIN      
        1 gctatatatt atggtatcga atcttcatca gaatgataaa attaatcaat tgatatctga
       61 ttacaaacag aatatgaaag ctttttatat cactattgaa aaatttatac gagatgatga
      121 aagccttaag tgttatttta taaaggttat ttcaagtcgt tccaaggtaa caagtctaga
      181 tcagattgaa gctgataaaa cgatacaaag aaaatattca agtgagctaa aaaaatttat
      241 tggattttat aatgagatta tttgtgagga aaatagtttc ctacatgtac gaaagaggtg
      301 gtcgagttgg tttaggtagt cgatgcgtga gttgataatt ctcagggtat ggacttcttt
      361 ttcatgaatg aggtaaaaga gcaggtattg tttagagaca atcattctga gcatattttc
      421 tggatagagg gagtatccga ttttatgatc aaagttaata ccgccctctg gtgagaagat
      481 gagtaggttg gtaatttaaa ctattaaaca gaatttttga ttaaaagtat tattttatga
      541 gagaaatcct aatttcacaa tccataggca aactcttgca tttcgttttt tattggacta
      601 taataggttg gtataaagcc ttttgtagta ataaaatgta gaaggtgtag aaagcaagga
      661 tttagaatat ttgtagttaa aaacacaatg ttgctattcc ttacgatagg gagatagata
      721 tggcaatgat agaagtggaa catcttcaga aaaattttgt gaagactgtt aaggaaccgg
      781 gcttgaaggg ggctttgcgc tcctttattc atcctgaaaa gcagaccttt gaagcggtca
      841 aggatttgac ctttgaggtt ccaaaagggc agattttagg atttatcggg gcaaatggtg
      901 ctgggaagtc gacaaccatt aaaatgctga caggaatttt gaaaccaaca tctggttttt
      961 gtcggattaa cggcaagatt ccccaggaca atcggcaaga ttatgtcaaa gatattggcg
     1021 tagtctttgg acaacgcacc cagctatggt gggatttggc tctgcaagag acctacactg
     1081 tcttaaaaga gatttatgat gtgccagact cgctctttca taagcgtatg gactttttga
     1141 atgaagtctt ggatttgaag gactttatca aggatcccgt gcggactctt tcactgggac
     1201 aacggatgcg ggcggatatt gcggcctcct tgctccacaa tcccaaggtt ctttttttag
     1261 atgagccgac cattggtttg gacgtttcgg ttaaggataa tattcgtcgg gcaattactc
     1321 agatcaatca agaggaagaa actaccattc ttttgaccac tcacgatttg agtgatattg
     1381 agcaactttg tgatcggatt ttcatgattg acaaggggca agagattttt gatggaacgg
     1441 tgagccaact caaggagacc tttggtaaga tgaagactct ctcttttgaa ctgctaccag
     1501 gtcaaagtca tctcgtctct cactatgacg gtctgtctga tatgaccatt gatagacaag
     1561 gaaacagcct caacattgaa tttgatagtt ctcgctacca gtcagctgac attatcaagc
     1621 aaaccctgtc tgattttgaa atccgcgatt tgaagatggt ggatacggat attgaggata
     1681 ttatccgtcg cttctaccga aaggagctct aggatg
//
LOCUS       DAVBHS010000206         1670 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVBHS010000206 DAVBHS010000000
VERSION     DAVBHS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.206"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..609
                     /locus_tag="VQ651_002000"
     CDS             <1..609
                     /locus_tag="VQ651_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HET2754945.1"
                     /translation="TVLSRLGDLSLPCPQDEDLTILPHSAYSETLYQAGSWSHKRRVC
                     QFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIKEMKEGFFGDKTDSS
                     TLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFRHLFLHVVGKCVRTGRKQLL
                     KLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPGRKASLMMH"
     gene            complement(1009..>1670)
                     /locus_tag="VQ651_002001"
     CDS             complement(1009..>1670)
                     /locus_tag="VQ651_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754946.1"
                     /translation="IIRKVQEPEFWKEVNLSDLEKIRLAIRDLLQFLDKTDRKPYYVN
                     FEDRILSTVHETTAFLQVNDLRSYNEKVEHYLKTHLDEESISKLYHNKKLTSDDMLAL
                     EKLLWEKLGSKADYQSHYENKAIPRLVREIIGLDRESANRIFSKFLSDENLNARQISF
                     VKLIVDYIVENGFLETKVLTQEPFKSYGSVQLLFQHQLPVLRNIVQIIELINNRAGEA
                     A"
BASE COUNT          521 a          333 c          309 g          507 t
ORIGIN      
        1 actgttctga gccgtcttgg agacctttcc ctcccttgcc cacaggatga ggacttaacc
       61 atcttgcccc actccgccta ctcagaaact ctctatcaag caggatcttg gtcgcacaag
      121 cgtcgtgtct gccagttctc tgaacgaaaa gaaggaaact tgttctacga tgttatttct
      181 ctcgttacaa atatgacgag tggaacaagc caagaccagt ttcagcttta tcgtggacgt
      241 ggtcaagccg agaatttcat caaggagatg aaggagggat tttttggcga taaaacggat
      301 agttcaacct taatcaaaaa cgaagttcgt atgatgatga gctgtatcgc ctacaatctc
      361 tatctttttc tcaaacatct agctggaggt gacttccaaa ctttaacaat caaacgcttc
      421 cgccatcttt ttcttcacgt ggtaggaaaa tgtgttcgaa caggacgcaa gcagctcctc
      481 aaattgtcta gtctctatgc ctattccgaa ttgttttcag cactttattc taggattaga
      541 aaagtcaacc tgaatcttcc tgttccttat gaaccacctg gaagaaaagc gtcgttaatg
      601 atgcattaaa gaacagtcga gatgaaaaaa tcgtgtgacg caccaaggga ggagtctgcc
      661 cttttgagga aatctagcga ggaaaaacga tactggaaca gcagaaagta aaactgacct
      721 catgaggagg aagaaagtgg ctcatgaggt caggggtttt gtaagttaca tctagttgag
      781 agaggtatga atgatttggg ttaaaatcaa agtagctctt ttgttaataa ctcgatcaac
      841 aaacatcgtg gtacaagtat ctactttgaa atttatcaac cacttaacaa ctgatactgt
      901 attctaggaa aacgatgaca ttcttcctaa taaaacttct catatatagc ataaatttct
      961 actcttttta attttaaatg agtctcagca tggcaatcac tttagaattt aagccgcttc
     1021 tccagctcga ttattgataa gttcaatgat ttgaacaata ttacgaagta ctggtagttg
     1081 gtgttggaag agtagttgaa cagaaccata agatttaaac ggctcttgcg ttaacacttt
     1141 cgtctctaaa aaaccatttt ctacaatgta gtctacaatc aattttacaa atgaaatctg
     1201 cctggcatta agattctcat ccgacaaaaa tttagagaaa atacgattgg cagactctct
     1261 atctaagcca ataatctcac gaaccaatct cggaattgcc ttattttcat aatgactttg
     1321 gtagtctgct ttactaccta atttttccca aagcaatttt tcaagtgcaa gcatatcatc
     1381 agatgtcaac tttttattat ggtatagctt agaaatggac tcctcatcca gatgagtttt
     1441 caaataatgc tcaacttttt cattgtaaga ccgaagatcg ttgacctgca aaaatgctgt
     1501 ggtctcgtga acagtggaga gtatacgatc ttcaaagtta acatagtagg gtttacggtc
     1561 tgttttatcc aaaaactgta ataaatctcg aatagcaaga cgaatttttt ccaaatcaga
     1621 caagttaact tctttccaaa attcaggctc ctgtactttc ctgataattt
//
LOCUS       DAVBHS010000207         1630 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVBHS010000207 DAVBHS010000000
VERSION     DAVBHS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.207"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            110..409
                     /locus_tag="VQ651_002002"
     CDS             110..409
                     /locus_tag="VQ651_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HET2754947.1"
                     /translation="MDSYAINFWESKGVGIDKKILEFIQKNITVIKNKNFLNKDFDIY
                     VKKIHNLFFVISTLVSKEQYMGIVSSQIHMFANRMGISPTIEVTMLDFFLREDSD"
     gene            411..1181
                     /locus_tag="VQ651_002003"
     CDS             411..1181
                     /locus_tag="VQ651_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HET2754948.1"
                     /translation="MEKYFFDISIFEKIKKVLSYQSKYWEISKPRDKYSFKSQNRISG
                     SNSIKFKDFELDYELESLGKILLKRRSFLLNEFDKNASFDKLIYLLKTVFSPTNCKFV
                     FGEEVPLFSYPTSGALNSITPFVYIDNMDEVKNGIYEVKYDGLEFKKSMNIIDLETFT
                     PITSKNSDVQNNSFKKVNMIIMLCSNYENIFNKYGLLAYRLLHLEAGHIAQNIQLVST
                     ALGIKSLPMGGWFDDKIETFINEISDKKNIYLYAIAVG"
     gene            1201..1365
                     /locus_tag="VQ651_002004"
     CDS             1201..1365
                     /locus_tag="VQ651_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754949.1"
                     /translation="MNAEDKFRELENENKLLKLHNKELENEIQNGWKFWIWVTVPILG
                     LIYAIIQKIF"
BASE COUNT          597 a          176 c          268 g          589 t
ORIGIN      
        1 agataactat tattacgaaa aaaaattctt gtgtaaaatt ctattgactt tctatattat
       61 ttttgtattt cataacgaat gtaatttaac aagtgaagaa attttaaaga tggattccta
      121 tgctattaat ttctgggaga gtaaaggggt cggtattgac aaaaaaatct tggaatttat
      181 tcaaaaaaat attacagtta ttaaaaataa aaattttttg aataaagatt ttgatattta
      241 tgttaaaaag attcataatt tattctttgt catttctact ttagtttcaa aagagcaata
      301 catggggata gtttcttccc aaatacatat gttcgcaaat aggatgggaa tttctcccac
      361 cattgaagtt accatgctag atttttttct gagggaggat tctgattaat atggaaaaat
      421 atttttttga tatttctatt tttgaaaaaa taaaaaaagt attatcatac caatctaaat
      481 attgggaaat atcgaagcct agggataagt acagtttcaa aagtcaaaat agaatatctg
      541 gctctaatag cattaaattt aaagattttg agttagatta tgaactggag tcacttggta
      601 aaatactttt aaaaaggaga tcgtttttac taaatgaatt tgataagaat gcgagttttg
      661 acaaactgat ttacttgttg aaaactgtat tctctcctac aaattgcaag tttgtatttg
      721 gagaagaggt tcctttattt agctatccta catcaggtgc gttaaattca attactcctt
      781 ttgtttatat tgataatatg gatgaagtaa agaatggtat atatgaagta aagtatgacg
      841 gacttgaatt taaaaaaagt atgaatatta tagacttaga aacatttacc ccaataacaa
      901 gtaaaaacag tgacgtacaa aataattcat ttaaaaaggt aaatatgata attatgttat
      961 gttcgaatta tgaaaatatt ttcaataaat atggattatt agcttatcga cttttgcatt
     1021 tagaggctgg tcatattgca caaaatatcc agcttgtttc tactgcgcta ggaataaaaa
     1081 gtttaccgat gggaggttgg tttgatgata aaatagaaac ttttattaat gaaattagtg
     1141 ataaaaaaaa tatttatctc tatgcaattg cagttggata aatttaagga ggaagtgtag
     1201 atgaacgcag aggataaatt tcgcgaacta gaaaatgaaa ataaacttct aaaacttcat
     1261 aacaaggagc ttgagaatga aattcaaaat ggatggaaat tttggatatg ggtaactgtc
     1321 cctattttag gtttgatata tgctattatt caaaaaattt tttaattatg ctagtttgtc
     1381 aggttgagta gtttttgttc aaaatttaat aaactttttt caatgaagat tatctaaaga
     1441 ttgcttctca gatgagaatg gcttctctat cttatataaa ttatcttcat agagtgtcct
     1501 gttattcatg ttatcacaga atagatagtt tttttggctc tataatattt gtagtgggta
     1561 aatcccctat agatattatg gagcctattt ttgtgtagaa aaaaagtccc atatcatcta
     1621 taatgaaaag
//
LOCUS       DAVBHS010000208         1605 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVBHS010000208 DAVBHS010000000
VERSION     DAVBHS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.208"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(144..1340)
                     /gene="tuf"
                     /locus_tag="VQ651_002005"
     CDS             complement(144..1340)
                     /gene="tuf"
                     /locus_tag="VQ651_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2754950.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
BASE COUNT          447 a          314 c          330 g          514 t
ORIGIN      
        1 tgtactattc tagtttcaat atattatagt ttgctctttg attttcattg agtattagtt
       61 caatatctaa aaaaagaacc ttgccaagca agattctttt agtgtctgac ttaaataatt
      121 gttcttctgg gaactaaatc gaattaagct tcgatttctg taaccatacc tgaaccaaca
      181 gtacgtccac cctcacggat agagaatgta gtaccttgtt ctacggcgat tgggtgaatc
      241 aactcaacgt cgattgtcac gttatcacca ggcattacca tttcagtacc tgctggaagt
      301 tcgattgaac ctgtaacgtc agtagtacgg aagtagaatt gtggacggta gttgttgaag
      361 aatggagtgt gacgtccacc ttcttcttta gtaaggatgt agacttcacc tttgaattta
      421 gtgtgtgggt tgattgaacc tggtttagcg ataacttgtc cacgttcgat ttcatcacgt
      481 tgaacaccac gaagaaggac acctacgtta tctccagcaa gaccttcgtc aagttgttta
      541 cggaacattt caacaccagt aacaactgct ttttgagttt cttctttgat accaacgatt
      601 tcgatttcgt cgttgacttt aacgatacca cggtcgatac gtcctgaagc aactgtacca
      661 cgtccagtga ttgagaatac gtcctcgact ggaagaagca atggtttgtc agtgtcacgt
      721 tctggttctg ggatatactc atcaactgtg ttcatcaatt ccataacgat gtcttcgtat
      781 ttagagtcac cttcaagagc tttaagtgct gaaccttgga taactggaag atcgtcacct
      841 gggaagtcgt attctgacaa taggtcacgg atttccattt caaccaattc aagcaattct
      901 tcgtcgtcaa ccaagtcaac tttgttcatg aagacgataa ggtgtttaac accaacctga
      961 cgtgaaagaa ggatgtgctc acgagtttgt ggcattggtc cgtcagttga agctactaca
     1021 aggatagctc cgtccatttg agcagcacca gtgatcatgt ttttaacgta gtccgcgtgt
     1081 cctggagcgt cgatgtgagc gtagtgacgt ttttcagttt cgtactcaac gtgcgcagtg
     1141 ttgatagtga taccgcgttc gcgttcttct ggagcagcat cgatagacgc atagtcttta
     1201 ggttggttaa ctgatgaagg caagcgacgt gccaaaacag ttgtgatagc tgcagttagg
     1261 gtagttttac cgtggtcaac gtgtccgata gtaccaatgt taacgtgtgg tttactacga
     1321 tcgtattttt cttttgccat ttgagtaaaa gcctccaata aaatatattt tatagataga
     1381 cagtaggcaa tacagtctaa ctttccttac tattttatca aatttcagct aaattgcaag
     1441 tgttttacaa cgtttttgtg aattattctt ggtaaaaatt taactaatta atagaaagta
     1501 ctaaatagac ttatccttca aggagttttc agcattatcc atccactcac ttgctgaaaa
     1561 tgtaaacagt ctctggttta tatagtaaaa tgaaacaaga ataga
//
LOCUS       DAVBHS010000209         1451 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVBHS010000209 DAVBHS010000000
VERSION     DAVBHS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.209"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            120..1271
                     /locus_tag="VQ651_002006"
     CDS             120..1271
                     /locus_tag="VQ651_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2754951.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
BASE COUNT          361 a          266 c          320 g          504 t
ORIGIN      
        1 aagggattca ttttcgaatt taagaaggag tctacaaacc aatgaggctt cttcttttct
       61 tgcttatttg ataactatca aaaaagtaga agattaagag aaatatagaa agagatttca
      121 tgaaacaatt tttagaacgg gccagcattt tggctctctc cctcgttttg attacctcct
      181 tttccatttc gagtgcccta ccagccatgt ttgactatta tcagggttat tctaaggaac
      241 aaattgagct cttggtgagc ttgccttcct ttggaatcat gatgatgtta ctgctaaatg
      301 gtttcttaga aaaaatattt cctgagcgct tacagattag tttgggcttg ctgattttat
      361 cattgagcgg tacagctccc ttctggtacc aagcctatcc ctttgtcttt ggaacacggc
      421 ttctctttgg tttgggtctt gggatgatca atgccaaggc catttctatt atcagtgaac
      481 gctaccaagg aaaaaggcga attcagatgt tagggctacg cgcttctgca gaggtcgttg
      541 gagcttctct cattaccttg gccgtcggtc agttgttggc ctttggttgg acagctatct
      601 ttctagccta tagtgctgga tttttggtgc tgccccttta tctgctcttt gtcccttatg
      661 gaaaatcaaa gaaagaagtc aagaaaagag cgaaggaagc aagtcgttta actcgagaaa
      721 tgaaaggctt gatttttacc ttagctatcg aagcggcagt tgtagtttgt accaatacag
      781 ctattaccat ccgtattcca agtttgatgg tggaaagagg attgggggat gcccagttat
      841 ctagttttgt tcttagtatc atgcagttga tcgggattgt ggctggggtg agtttttctt
      901 tcttgatttc tatctttaaa gagaaactgc tcctctggtc tggtattacc tttggcttgg
      961 ggcaaatcgt gattgccttg tcttcatcct tgtgggtggt agtagcagga agtgttctgg
     1021 ctggatttgc ctatagtgta gtcttgacga cggtctttca acttgtctct gaacgaattc
     1081 cagctaaact cctcaatcaa gcaacttcat ttgctgtatt aggctgtagt ttcggagcct
     1141 ttacgacccc atttgttcta ggtgcaattg gcttactaac tcacaatggg atgttggtct
     1201 ttagtatctt aggaggttgg ttgattgtaa tctctatctt tgtcatgtac ctacttcaga
     1261 agagagctta ggattgattc ctaagttttt cttttgatac tcaatgaaaa tcaaagagca
     1321 tagttgattg agtttggaat agtacgctgt agctgctaaa atatttctag aaattaattt
     1381 gacttttcta atagagttat tcatatctta tttcaatcta ctatagaaag ctagccgcag
     1441 gttgctcaaa a
//
LOCUS       DAVBHS010000210         1422 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVBHS010000210 DAVBHS010000000
VERSION     DAVBHS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.210"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            363..950
                     /locus_tag="VQ651_002007"
     CDS             363..950
                     /locus_tag="VQ651_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754952.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGTLLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQKKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            complement(1037..1240)
                     /locus_tag="VQ651_002008"
     CDS             complement(1037..1240)
                     /locus_tag="VQ651_002008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754953.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
BASE COUNT          408 a          241 c          275 g          498 t
ORIGIN      
        1 aatacgtcta tttatttttt attgtttttt attgtagagc agacccaagc gaatgaacat
       61 aggttgtaaa aagcaaatga aaaaactgcg aggataatag cttctgtgtc taaataaccc
      121 atttcgagac ctcttttcaa caacaacaag cctgtagttt cgataacgat caaacataaa
      181 aatatataaa aaattttcgc attaagcata ttgttaccgc tttcttttta tttggtttta
      241 ttgtataata aaagttgaaa aaaatcaaaa atacaaagga gtttccgtta tgtctaagat
      301 gtttaaatgc atgtgtttgc tagttatgtc tgttttctgt atgtctagca tttccccgtc
      361 gcttgtttgt ttttgctgat gatagcttat cggcaaactc taaagttgta tctggtgagg
      421 ctcagtttga gaatggctca tcagtgcggt tcggagatac gcaagtaaat attttaagcg
      481 atgaagttct tgaggttgtc aatccagatg gaagtgtaga cactattgaa cgacgtgctg
      541 acggcgtcta tatcaatggg gcattttata tggcttatca aaagaatgaa atcgacctaa
      601 atatatcttt tagatcatac gatcctaatg tttggaatta cgtcaacaca atacatggga
      661 ataaacaagc taacacattc gctaacttca tgactggtgc tggaatcagt tatatgattg
      721 gtaggatagg tactttattg gggggtccat ggggtgcaat aattggtgga gcatattttg
      781 gtatacaagc ttatcagtct tacttggatt cacaaagccc atatccatac tacataacaa
      841 gtacttatat ccatgttgct caaaaaaaat ggaagtttat cactgaatac tatagaaact
      901 caaactatac tgggtatgta aaaaccgtaa caacctacgt gaatttctaa aatataaacc
      961 ctgcctagat ataaaggcag ggtttatatt ttagttaatt gctcaagtaa tttgtaagca
     1021 taatacttgc gaaactctat ttgttcaagc ggttcaaaag ctcttgtttt ttatcctcaa
     1081 gctcagctct cctttgctcg atttcttcac gtttttgggc gatttctgct tgcgcctcgg
     1141 gagtttccaa agtgccttgt gacttgtgaa attctactag catatcaacg atttttgggt
     1201 ctaattcgtt gacataatca ggatttgacc atttcggcac gttgcttgca ggtgcttgct
     1261 ttttgccgcc ttttttgggc gttttggctt gttgttcagc tttctgactg tagttgacct
     1321 cgtggaattt tcgtgcttga tatgatgcgt tttattatga gaagatttac ttcatttttt
     1381 gctgaaattg ggctcttgcc caagctctat caacataatt gt
//
LOCUS       DAVBHS010000211         1374 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.211, whole genome shotgun sequence.
ACCESSION   DAVBHS010000211 DAVBHS010000000
VERSION     DAVBHS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.211"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            255..1265
                     /gene="ccpA"
                     /locus_tag="VQ651_002009"
     CDS             255..1265
                     /gene="ccpA"
                     /locus_tag="VQ651_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HET2754954.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKVGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
BASE COUNT          432 a          222 c          316 g          404 t
ORIGIN      
        1 tttagaaggg cttagtttcc tcctttttat ttagtctaag gtggtagcaa ggagctaaac
       61 ctaggttttc tatgaaattt tttcaatcaa tcttcaaaaa acacttgaaa gtgtttacaa
      121 ttagtgatag aatggaataa gtagaaatat gaaaacgaaa ttttcacatc ttcacttttg
      181 taagtagata tagagcttca atcagtacct ctacttgtaa aagaatgtgt gagatagaaa
      241 ggacaggaaa aggaatgaat gcagatgata cagtaaccat ttatgatgtc gctcgtgaag
      301 caggtgtttc catggcgacg gtcagccgtg tggtcaatgg caataaaaat gtaaaagaga
      361 atacccgtaa aaaagtgcta gaggtaattg atcgtttgga ttatcgtcca aatgcagttg
      421 cgcgtggtct tgcaagtaaa aagacaacca ctgtcggtgt cgtgattcca aatattacca
      481 atggttattt ttcgagtttg gctaagggga ttgatgatat tgcagaaatg tacaagtaca
      541 atattgtcct agctaatagc gatgaagata acgagaaaga agtttctgtt gtcaataccc
      601 tcttttcaaa gcaggtagat ggcattatct atatgggtta tcacttgaca gataaaattc
      661 gctcagaatt ttcgcgttca cgtactccga ttgttctcgc aggaactgtc gatgttgagc
      721 accagttgcc aagtgtcaat attgactata agcaagcaac aattgatgca gtgagctacc
      781 ttgctaaaga aaatgagcgt attgctttcg ttagcggtcc gctagtggat gacatcaatg
      841 gtaaggttcg tttagttggc tacaaggaaa ccttgaaaaa agtaggaatc acttatagtg
      901 agggtttggt atttgaatct aaatatagct atgatgatgg ttacgcctta gcagagcgtt
      961 tgatttcatc aaatgcaact gcagcagttg tgacaggtga tgagttggca gcaggagtct
     1021 tgaacggttt ggctgataag ggtgtttctg tgccagaaga ttttgaaatt attactagtg
     1081 atgattcaca aatctcacgc tttacccgtc caaacttgac aacgattgcc caacctcttt
     1141 atgaccttgg tgccattagt atgcgtatgt tgaccaagat tatgcataag gaagagttgg
     1201 aagaacgtga agttctctta cctcatggtt tgacagaacg tagctcaaca cgaaaacgta
     1261 aatagaaaaa atcagggaat cgagaagatt tcctgatttt gtgctcttaa acaagagatg
     1321 ttgacttaat gaataccata gagcggacta ggcggctggt taaaggctgc gcaa
//
LOCUS       DAVBHS010000212         1346 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.212, whole genome shotgun sequence.
ACCESSION   DAVBHS010000212 DAVBHS010000000
VERSION     DAVBHS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.212"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(129..269)
                     /locus_tag="VQ651_002010"
     CDS             complement(129..269)
                     /locus_tag="VQ651_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754955.1"
                     /translation="MKFFWTILAILFIKPIIGIVKFFWMIISFAVQLLFYKILDWFFK
                     LI"
     gene            complement(266..448)
                     /locus_tag="VQ651_002011"
     CDS             complement(266..448)
                     /locus_tag="VQ651_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754956.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKDDHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(450..1233)
                     /locus_tag="VQ651_002012"
                     /pseudo
     CDS             complement(450..1233)
                     /locus_tag="VQ651_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          439 a          287 c          201 g          419 t
ORIGIN      
        1 gctgacgtgg tttgaagaga ttttcgaaga gtataaaatg attttactgc ttatattata
       61 ccatatggag agagattaaa aaactagcag tggagttcct gctagttttc tgcgacttgg
      121 attaccatct agataagttt aaagaaccaa tccaatatct tgtaaaacag caattgaact
      181 gcaaaagaaa tgatcatcca aaagaatttc acaatcccaa taatcggttt gataaaaaga
      241 atagcaagaa ttgtccagaa aaatttcatt ctctctcctc tggcttaatg agccaccgag
      301 ccgtatccat tagcttatct gcaataaaga gttttcccag accaacaacg acatggtcat
      361 ctttctttat cgttttcatc acttttacac cttgcacggt caaaaagtaa ttaccataaa
      421 ttttagcaaa agctttgata ggattcatat tattctcctt tgatgatttc agcctctttt
      481 cggattgttt caacatcttc ttgatattga acttcactat agttgactag cttggataat
      541 tctttctgca agctttcccc cattggtttc atagttgcaa aggttaaacc acctgaaatg
      601 ataccaccca agataggaat aaatttcccc attcctttgg ccaaccctcc cttggtaaga
      661 ttcacaccaa atatttttaa gacttttttc aaaatagggt accaaagcgt ctttgttaaa
      721 gctttattag gcactgtttt cattacctgt ttggcaattg ttataccacc agcacgtagc
      781 aaacaaagca gcggttccat tcacccctaa cattacgcct agataaagca agagggtatt
      841 ttgagcatct tcactcaact cctcacgtga agcccaaaga tcctcataac cataaatata
      901 acctaattct tgggccaatt tcagagagaa accataaaat tgagccacat aagctggaat
      961 ggtaattgcc atagcaagcc ctccaggtaa tcctgccaaa acagaagtcc cactcgctaa
     1021 taaaacatta tccctaatac aggcattagc cactcgatct aagatttctt gagataagag
     1081 agacgttgga ccttgttcca ataaggtagg aatgtcctgt ggacccaatt ctttggaaaa
     1141 cttatccact aaaaatttcg aacgatcaac cttaacaact ggtagtttta ccacttgttg
     1201 taatacttgc atagctaagt cttgttcagc catatacaaa ctccttattt cttttttgca
     1261 ccttatcata ttatatttcc tacaaaaaaa gcattttcta attagataac attaaaaaat
     1321 acagaacccc ttttttatag taaaat
//
LOCUS       DAVBHS010000213         1338 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.213, whole genome shotgun sequence.
ACCESSION   DAVBHS010000213 DAVBHS010000000
VERSION     DAVBHS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.213"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(295..687)
                     /gene="rpsI"
                     /locus_tag="VQ651_002013"
     CDS             complement(295..687)
                     /gene="rpsI"
                     /locus_tag="VQ651_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET2754957.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(707..1153)
                     /gene="rplM"
                     /locus_tag="VQ651_002014"
     CDS             complement(707..1153)
                     /gene="rplM"
                     /locus_tag="VQ651_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET2754958.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
BASE COUNT          378 a          264 c          267 g          429 t
ORIGIN      
        1 tcgatttgac tatcctcggt ttgtcctatt cttatttcac cttactataa aaataaggta
       61 aacttttcaa aaaaacacga aaaaagcacc catagggcaa acctttgagt gctttgaaac
      121 attgatataa ttgtattgat tagcgttttg aggcaactga ccttgtcagg ttgcagccac
      181 atttgtaagc aactaaagtc gcaacaactg tgtcaattgc accaatttag taagaaagta
      241 taaaaaaaga acaccccgaa aggtgctctg tataagtata gtaattcttt cgaattaacg
      301 tttactaaat tgtgatgctt tacgagcttt cttaagacct ggtttcttac gttcaacttt
      361 acgtgagtca cgtgtaagaa gtcctgcgcg tttcaatgaa tcgcggaagt ctgggtctac
      421 ttgaagaagg gcacgagcga taccgtgacg gatagctcct gattgaccag cgtatccacc
      481 acctacaacg ttaacgaaaa cgtcgtatga acctacagtt gaagtaactg cgaatggttg
      541 gttgatgaca agacgaaggt cagcatgtgg gatgtactct tcaacatctt ttttgttaac
      601 agtgatttta ccagttcctg gaacaaggcg aacgcgtgca acagcgtttt tacgacgtcc
      661 agtacctgca tattgtgctt gtgacatact ttatttgttc ctttccttag ataagtcctg
      721 aaatgtcaag aacttctggt tgttgtgcag catgagtgtg ctcagctcca acaaatactt
      781 tcaacttcat accttgagcg cgtccaagtg tattgtgtgg aagcatacct ttaactgatt
      841 tctcaatcaa acgtactgca tttttagaac gaagttcacc tgcagagatt tgtttcaatc
      901 cacctgggtg gtttgagtga gtgtagtaga ttttatcagt tgctttttta ccagtcaatt
      961 taactttttc agcattgata acaatcacga agtcacctgt atcagtgtgt ggtgtaaatg
     1021 ttggtttgtt ttttccgcga agtacgctag caactactgc agaaagacgt ccaagtggta
     1081 catcagttgc gtcaactacg taccatttac gttcaacttg gcctggttta gccataaatg
     1141 ttgttttgtt catgatttct cctatatata gttcgatttt tgtttacggt gatgggtgtt
     1201 ccttcccatc gaaaagtatt tggaaggttc cggggccttt caaatggggt aaacaatacc
     1261 gcctactatc ataccaaatt tcacccctaa aagtcaatag atatgaaaaa tatttttcgg
     1321 taacttctca aagtaact
//
LOCUS       DAVBHS010000214         1285 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.214, whole genome shotgun sequence.
ACCESSION   DAVBHS010000214 DAVBHS010000000
VERSION     DAVBHS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.214"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(1..459)
                     /locus_tag="VQ651_002015"
                     /pseudo
     CDS             complement(1..459)
                     /locus_tag="VQ651_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(695..1006)
                     /locus_tag="VQ651_002016"
     CDS             complement(695..1006)
                     /locus_tag="VQ651_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754959.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYVCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(1021..>1285)
                     /locus_tag="VQ651_002017"
     CDS             complement(1021..>1285)
                     /locus_tag="VQ651_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HET2754960.1"
                     /translation="TATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRA
                     PNPMELLTIKVPQPVQAGDMVRALKEGLINLYKEDGTSVTVRA"
BASE COUNT          391 a          268 c          219 g          407 t
ORIGIN      
        1 cgcatcatca taagaaacca cttcagcgac aaacttgtac tcagggattt tacgacgagc
       61 accaaacaac tgctcatttt cagatggtgt accatagtaa aatcctgtag ccagttcacg
      121 ttgggcaacc ttccacatct cgtccaccaa gtcttgtttg atagcttcaa acttttcagg
      181 actttcaaga taggcatcca caaccgcctt gtagcagttg gttactgtta atacatagtg
      241 aatagactcc atacgtcctt cgatttttag actgtccaca ccattttcaa tcatatctgg
      301 aatgtggtca atcatagaca tgtcaacggc tgacattgaa aattcttctg gaatctcacc
      361 ctgcaaactc ttacgttctt tcccaaatgg catatcgtaa aggtcgtatt tccaacggca
      421 tgactgagaa catccaccac ggttggcatc acgcatactc tagacgcaat tctaatcttt
      481 tagactttgc ataataactg ttaaacagtt gaaaattaac ccagttttaa caagtagtca
      541 aaataccaga taaaggaaaa aaagtcttta aaatcttaaa aacgcatagt atcaggtatt
      601 gaacaatctt gatattatgc gttttattgt gggaagattt acttcatttt ctcttggaat
      661 tgagttttct cctacttcaa aactagatga tttgctattt aaaatagcgt tccttatgat
      721 tttggcaacc tggattaaag cgagaaaaac aaaaggggca acttaagctt tctttatatt
      781 gcttatatgt tagtagtttt agacaaacac cacataaaat aggcttatcc tgtatcaaag
      841 ataagggata cggctcaaaa gggtgatttt ctaatgaatc atgacaccga taacaagcat
      901 agtacttttt acaaacatag cattgtaaag caataatatc cagcttgcta tggtaatgaa
      961 tgcatcttgt ttgcttatcc actaagaaac cctgtgcctc tatcatttcc ttttcctttc
     1021 ttaagctcga actgtgacgc tggttccatc ttccttataa agattgatga gtccttcttt
     1081 taatgcacga accatgtctc cagcttgaac aggttgaggc accttaatag tcaatagttc
     1141 cattggattt ggagcgcggt cgattttatt gcctttggca tcatgcaaat cttcaatata
     1201 ggtttcaaaa tgacggaaac ctggaccata aaactcaact tggtcccctt cgttaatgac
     1261 gtttcgttga cgaatagttg ctgtt
//
LOCUS       DAVBHS010000215         1251 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.215, whole genome shotgun sequence.
ACCESSION   DAVBHS010000215 DAVBHS010000000
VERSION     DAVBHS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.215"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            153..1184
                     /locus_tag="VQ651_002018"
     CDS             153..1184
                     /locus_tag="VQ651_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET2754961.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGRALFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNAGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
BASE COUNT          372 a          257 c          256 g          366 t
ORIGIN      
        1 tgctcaaagc acagctttga ggttgcagat aaaactgacg tggtttgaag agattttcga
       61 agagtattaa aagtattctt ctgaaatccc acatagcttt ctcttatatt ttgtgataaa
      121 ataggctcaa tctatttcta ggaggatgag atatggtttc tactattggt attgttagtt
      181 tatctagtgg cattatcgga gaggattttg tcaaacacga agtggacttg ggtatccaac
      241 gtctcaagga tctgggactc aatcccatct ttttgcccca ttcgttaaaa ggattagact
      301 ttatcaagga ccatcctgaa gctcgtgcag aggatttgat tcatgccttt tctgatgata
      361 gcatcgacat gatcctatgt gccatcggtg gagacgatac ctatcgcttg ctaccttatc
      421 tttttgaaaa tgaccaactc caaaaggtta tcaagcaaaa aatttttctt ggcttctcgg
      481 atacaaccat gaaccacctc atgttgcata aattaggaat caagactttt tatggtcaat
      541 cctttttagc agacatttgt gaattagaca aagaaatgct agcctatagc cttcactact
      601 ttaaagaatt gattgagacg ggaagaattt cagaaatccg ccctagtgac gtttggtatg
      661 aggaacgaac tgattttagt cccacggccc tgggaacacc tcgtgtcagt catacaaata
      721 caggttttga cttgttgcaa ggaagggcac tattcgaggg gaaaatcctc ggtggttgcc
      781 tcgaatccct ctacgatatc tttgacaact ctcgatacgc agatagcacg gagctctgcc
      841 aaaaatacaa acttttccct gacttgtcag actgggaagg gaaaatcctc ttgctcgaaa
      901 caagcgaaga aaagcctaag ccagaagact tcaaaaagat gttgctaact ttaaaggaca
      961 ctggcatatt cgcggtcatc aatggactct tggtcggaaa acctatggat gaaactttcc
     1021 atgacgacta taaagaggca ctattggata tcattgacag caatatcccg attgtctata
     1081 atctgaatgc cggccacgca actccaagag caattgtacc ctttggggtc catgctcatg
     1141 tagatgcaca ggaacaagtc attcgctttg actataacaa ataaagctgg gaaaagtttc
     1201 tcagcttttt tctcggtatc attactggca ttttcatcac atattacagc a
//
LOCUS       DAVBHS010000216         1190 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.216, whole genome shotgun sequence.
ACCESSION   DAVBHS010000216 DAVBHS010000000
VERSION     DAVBHS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.216"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            239..>1190
                     /locus_tag="VQ651_002019"
     CDS             239..>1190
                     /locus_tag="VQ651_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2754962.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
BASE COUNT          413 a          210 c          249 g          318 t
ORIGIN      
        1 agccacctta ccttagtcaa gaattagatg tttcactatg tttgagtaag ttgatgattt
       61 catttgataa caggtttgaa actgttaggc taggtggcca aggctaatca tagccttggt
      121 ttagctgaaa aaacaggttc aagggttcct gttgtcaaat gaaatgtgat ttaaggtata
      181 agaaaacacc tctgtgctat acttgttgtt caccacaaac acaaggaaag gcacagagat
      241 gcaagaacat tataccccaa aagggaaaca tttgacaata gataaccgtc gcttgattga
      301 gcggtggaag aatgaaaata agtccaatcg tgaaattgca ggcttgttag gaaaggcgcc
      361 tcaaacgatt cataatgaag tcaaaagagg tacaacttta caacaagtga gaaaagggct
      421 atacaaaaag gtctattctg ccgattacgc acaaactgtt taccaattca atcgaaaacg
      481 gtcggtgaaa aagttaattt taacaaagga aatcagagag aagatcttac actatcataa
      541 gcaaaaattt tcgcctgaaa tgatggttaa caagaagcaa gtgaaagttg gtatttcaac
      601 catctactac tggtttcata atggtcattt aggattgacg aaggccgaca tgctttatcc
      661 cagaaaaagg aaaggtgtca agaagcaagc tagtccgaac tttaagccgg caggtaaatc
      721 tatcgaagaa cgtcctgacg ttattaatct tcgcttggaa aatggtcatt atgaaattga
      781 taccgtccta ctgactaaga taaaaaatta ttgcctgtta gtcttaaccg accggcggag
      841 cagacaccaa attataaggt taattccaaa taaaactgct gaatctgtca atcaggcgct
      901 tacgttacta ttaggggagc atcgtattct gtccattact gcagataatg gttcggagtt
      961 caaacgattg tctgaggtat ttcctgagga acatatctac tacgcacatg cttactcttc
     1021 atgggagaga ggttcaaatg aaaatcataa tcgattaatt cggagatggt tacctaaagg
     1081 aaccaagaaa acgactccga aagaagtagc ttttatcgaa aattggatta acaactaccc
     1141 taaaaaatgc ttggactaca agtcgccaag tgaatttctt ttgggtggct
//
LOCUS       DAVBHS010000217         1154 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.217, whole genome shotgun sequence.
ACCESSION   DAVBHS010000217 DAVBHS010000000
VERSION     DAVBHS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.217"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            187..627
                     /locus_tag="VQ651_002020"
     CDS             187..627
                     /locus_tag="VQ651_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2754963.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            624..1082
                     /locus_tag="VQ651_002021"
     CDS             624..1082
                     /locus_tag="VQ651_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HET2754964.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
BASE COUNT          354 a          262 c          218 g          320 t
ORIGIN      
        1 caaactagaa agctaggcgc agacagctca aaacactgtt ttgaggttgt agataaaact
       61 gacaaagtca acaactatcc tacggcaaga cgacgctgac gtggtttgaa gagatttttg
      121 aagagtatta ataaaggtgt acaagaagtg agattaattt tgtatgaaaa acaaggagag
      181 aaatttatga aagtagaatt acagattagt gagacttacg aggaggaaaa gctgattgtc
      241 caagcacctc agccgacaga taaagttcag aaagtcatcg agttcgcaga aaatctggac
      301 caaagagaaa aaatcaaagg gaaaatcgat gatcaggtct atctagttga gattggcaag
      361 attcagcgct tctatatcga gaatcggaag gttctagcag aaaccgcgag tcagacctac
      421 agcattgatt tgcgactcta tcaggttctt aaactcttgc caaccaattt tatccaaatt
      481 tcccaatcag aaatcatcaa tatcgattcc atctctcacc tcaagctcac gccaaacggt
      541 ctggtagaaa ttttcttgaa aaacgaaagc ttcacctact cttcacgccg ttatctaaaa
      601 accatcaagg agaaattaga actatgaaaa aacaagtatt tcacgatgca gctgccggtg
      661 ttcttatcgg cctcatcctc tctatcctct tttcactcat ttatgcacca aatacctacg
      721 caccactaaa tccctactct ctcatcggcc aagtgatgga tcagcatcag gttcacggtg
      781 ccttggtctt gctctactgc acacttatct gggcagccat tggtatgctc ttcaactttg
      841 gcaatcgctt attcagccgt gactggagca tgcttcgtgc cactctgact catttcttcc
      901 ttatgctggc tggctttgtc ccactagcaa ctcttgctgg ttggttccct ttccactgga
      961 ttttctacct ccagctcatt atcgagtttg cgattgtcta tctcatcatc tgggctattc
     1021 tctataaaag agaggctaaa aaagtagatc acatcaatca actcttggag catagaaaat
     1081 aggaacctca taataagcaa atccgagaaa gaaacttctc ggtttttctt tatcctgctg
     1141 caccttacta tagt
//
LOCUS       DAVBHS010000218         1107 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.218, whole genome shotgun sequence.
ACCESSION   DAVBHS010000218 DAVBHS010000000
VERSION     DAVBHS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.218"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            15..1076
                     /gene="dinB"
                     /locus_tag="VQ651_002022"
     CDS             15..1076
                     /gene="dinB"
                     /locus_tag="VQ651_002022"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET2754965.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
BASE COUNT          362 a          217 c          232 g          296 t
ORIGIN      
        1 aaaaggtgct aggcatgttg atttttcctt tgttaaatga tttgtcaaga aaaatcatcc
       61 atattgacat ggatgccttt tttgctgcag tggaaatcag ggataatcct aaactcagag
      121 gaaaacctgt cattattgga agcgaccctc ggcaaacagg tggacgggga gtcgtttcta
      181 cctgtagtta tgaggcaaga gcttttggtg tccattctgc catgagttcc aaggaagctt
      241 atgaacgttg tccccaggct gtctttatct cagggaatta tgagaaatac aagtctgtgg
      301 gactccagat tcgagctatt tttaagcgct atacagattt gattgaaccc atgagcattg
      361 acgaagccta tttggatgtg acagaaaata aactcggtat caagtcagcg gtcaaaattg
      421 ctcgcctcat tcaaaaagat atctggcaag aactccatct aactgcttcc gcaggcgttt
      481 cttacaacaa attcttagct aaaatggcga gtgattatca aaaaccacat ggtttgacag
      541 tgattctacc tgaacaggct gaggattttc tcaaacaaat ggatatttcc aaatttcatg
      601 gagtaggaaa aaagacagta gaacgtcttc atcaaatggg cgtttttact ggtgctgatt
      661 tacttgaagt tcctgaggta accctaatag accgttttgg tagactaggc tatgatctgt
      721 atcgaaaggc tcgtggcatt cacaactctc cagtcaaatc caatcgcatc cgtaaatcaa
      781 tcggcaagga gaaaacctac gggaagattc tccttgctga ggaagatatc aaaaaagagc
      841 tgactcttct atcagaaaaa gtcgctctca atctacatca acaagaaaaa gctggaaaaa
      901 ttgtcatttt gaaaatccgc tacgaggact tttcaactct taccaaacga aaaagtcttg
      961 ctcaaaaaac acaagatgct agtcagataa gccaaatagc cctgcaactc tatgaagaat
     1021 taagcaagaa agaaagaggt gtccgcctat tggggattac catgactgga ttttaaagct
     1081 caatgaaaat caaagagcaa actagga
//
LOCUS       DAVBHS010000219         1086 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.219, whole genome shotgun sequence.
ACCESSION   DAVBHS010000219 DAVBHS010000000
VERSION     DAVBHS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.219"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(72..>1086)
                     /gene="lctO"
                     /locus_tag="VQ651_002023"
     CDS             complement(72..>1086)
                     /gene="lctO"
                     /locus_tag="VQ651_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HET2754966.1"
                     /translation="DTFTLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPII
                     MAPVAAHKLANEQGEVATARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFS
                     KDDGINRHIMDRVKAEGYKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGA
                     GKSMDFVYKSAKQRLSPRDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNH
                     GGRQIDGGPAAFDSLQEVAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVI
                     YGLALGGSVGVRQVFEHLNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDL
                     KLY"
BASE COUNT          314 a          263 c          231 g          278 t
ORIGIN      
        1 ttttcgatag caatttattc ttccaataca cgaagaaaaa cctccacatt cagtggaggc
       61 aatctgtttt atcaatacaa ttttaagtca cgagggtcaa ctgggaaggt tgggttgtat
      121 ggattgtgac ggagcttaaa gtgtttgaca tcttcaatgg tctgagctcc agacaattgc
      181 ataactgtct tcaattccgc attcaagtgt tcaaagactt gacgcacacc gacactacca
      241 ccgagagcca agccataaat aacagggcga ccaatagcta ccaagtctgc tcctgaagcc
      301 aaggctttaa agacgtgttg accacgacga acaccagagt caaagacaat cggcacacgt
      361 ctatcaactg cttctgccac ttcttgaagc gagtcaaagg cagctggtcc accgtcgatt
      421 tgacgaccac cgtggttggt tacccagata ccagaagctc ctgcagcaag tgaacgttca
      481 acgtcctcac ggcattgtgg tcccttgaca tacacaggaa gaccagagta ttcagcgata
      541 aattctacat cgcgtggaga caagcgttgt ttagctgatt tgtaaacaaa gtccattgat
      601 ttaccagcac cttctggcag gtattcttca acaatcggca tgccaactgg gaagacaaaa
      661 ccattacgct tatccacttc acgattcccc cctacagtag catctgccgt caagacaatc
      721 gctttataac cttcagcctt cacacggtcc atgatgtggc ggttgatacc gtcatcctta
      781 ctaaagtaaa attggaacca atggggtgtc ccttgaaggg cttcagaaat ctctggaagg
      841 tcaacagtag agtaagagct ggttgtataa agagaaccaa actcatgcac accacgcgca
      901 gtcgccactt ccccctgttc atttgccaat ttatgagccg caacaggcgc cataatgatt
      961 ggtgaagata gtttttcacc tgcaaattca atctctgtac ttggattttc aacatcgcaa
     1021 agtgtatgag gaacgatgag tttgtggtta aaggcgcgga tattctctct taaagtgaaa
     1081 gtatct
//
LOCUS       DAVBHS010000220         1054 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.220, whole genome shotgun sequence.
ACCESSION   DAVBHS010000220 DAVBHS010000000
VERSION     DAVBHS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.220"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..555
                     /locus_tag="VQ651_002024"
     CDS             <1..555
                     /locus_tag="VQ651_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2754967.1"
                     /translation="KDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTE
                     IPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIPA
                     THNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPIS
                     NHEEMKRIISKVDLLFQKVSQLFE"
     gene            complement(503..1048)
                     /locus_tag="VQ651_002025"
     CDS             complement(503..1048)
                     /locus_tag="VQ651_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2754968.1"
                     /translation="MNWVVIKIKDIFSINTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKVNQLWK"
BASE COUNT          380 a          165 c          153 g          356 t
ORIGIN      
        1 aaagacgaaa caacctctta tcctatatat gaaatcccag aagcatggag atatattaaa
       61 tttgctagcc tagttaactt cagaatagga aaaactcctc cacgcagtga agctactttt
      121 tggggaactg aaataccttg ggtatctata tcagatatgc ctatatctgg ttatgtaact
      181 aatacgagag aatctatttc taaattagca ttaaaatcaa agaaaataga catttcacct
      241 aaaggaactt tattaatgag ttttaaatta tctattggaa aagttgccat attggatatt
      301 cctgccactc ataatgaagc cattatatcg atattcccct atgctaataa agaaaatatt
      361 atcagagatt acttaatgat atttttgcca cttatctcta ctttaggtga ttcaaaagat
      421 gctataaaag ggaaaacatt aaatagtacc agtatctccg aattattaat ccctatttct
      481 aatcatgaag agatgaaaag aatcatttcc aaagttgatt tactttttca aaaagtttct
      541 caactttttg agtaataagt tcctgttcct caaaaggagc taacggaatt aatagctcgc
      601 ttagtgtagt tttaggaata ttatataaag cttgacctga tagtttagtt attgctttca
      661 attgtttata aaataacgga gaggacaagt taaataatag aaattttgaa ataatctctg
      721 aactttcgaa tggtgttaat tggaaaataa atccaccagc cacaacacca tcatagtctt
      781 tatcgattct tgcaaacttt ccaatatgtt ctaaagaggt tgatacaggt gttattagct
      841 gattatgttt taaataaact tgctcagagg agatgaattg tgtatcaatg tagtaatcat
      901 tatccaacag agaaaattct aaaggcttaa tattaccacc acgtataatt ctaacacctt
      961 tattattaat gcttaaatcg cccttcttgt aagaaagacc tgtatttatt gaaaaaatat
     1021 cttttatttt tataacaacc caattcatag gtat
//
LOCUS       DAVBHS010000221         1035 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.221, whole genome shotgun sequence.
ACCESSION   DAVBHS010000221 DAVBHS010000000
VERSION     DAVBHS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.221"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..990)
                     /gene="ltrA"
                     /locus_tag="VQ651_002026"
     CDS             complement(<1..990)
                     /gene="ltrA"
                     /locus_tag="VQ651_002026"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET2754969.1"
                     /translation="MSKLLDKILSRDNMLEAYNQVKSNKGSAGIDGITIEEMDGYLRQ
                     NWRLTKELIKQRKYKPQPVLRVEIPKPNGGIRQLGIPTVMDRMIQQAIVQVISPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAAAKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVWRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSL"
BASE COUNT          270 a          226 c          183 g          356 t
ORIGIN      
        1 caatgagaaa tagtttatcc atcctcgaat agacaaattc agttgctcaa tacgtcttgt
       61 taggtctata ctccatttcc tctgtgttag tttcttcaat ttaagcttaa atctccaaac
      121 actatcttga tgtggacgac ttttccaacc atctgatgat ttccagaacc cgaaacctag
      181 atatttcaac tctcttggtc tggtaatctt agtcttggtc atgtttactt tcagacctag
      241 ccgtttctca ataaaacgac tgactgaata catcacacgc ttagcggctg cctcgcttcc
      301 gaccgtaatc acacaatcgt ctgcgtagcg aacaaatcga agccctctct tttctaattc
      361 cttgtccaat tcattaagca tgatattgga taagagagga gataaatttc ctccctgtgg
      421 cgtgccaact agcgttttat aacgttgacc gttaatgata acacctgaat gaagatattt
      481 acgaatcaag gattccgtat ctccgtcttc gataatgtta tgtactaagg acatcaatct
      541 atcttgagga actgtatcga aaaatttctc taggtctatg tccactatcc actcatagcc
      601 gtcatttaag tactctaaga gcttcatgat ggctttttca catgacctat ttggtctgaa
      661 tccatagctc gtatctgaga aatggggttc acaaataggg ctaataactt gaacaatggc
      721 ctgttgaatc attctatcca taactgttgg aattcctagt tggcggatgc ctccgtttgg
      781 tttgggaatc tcaactctaa gaactggttg aggcttatat tttctctgtt ttatcagttc
      841 cttagtcagg cgccagtttt gtctgagata gccatccatc tcttcgatag taattccatc
      901 aatcccagct gagcctttat tagattttac ttgattgtag gcttcaagca tattgtcgcg
      961 tgataatatt ttatctagca attttgacat gtgcctattc ctttcttgtt ttggtgcctg
     1021 agggacatct tatat
//
LOCUS       DAVBHS010000222          984 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.222, whole genome shotgun sequence.
ACCESSION   DAVBHS010000222 DAVBHS010000000
VERSION     DAVBHS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.222"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            13..366
                     /locus_tag="VQ651_002027"
     CDS             13..366
                     /locus_tag="VQ651_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET2754970.1"
                     /translation="MILRHLGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAKNVTIL
                     EGSNIHVLPSMPYSAQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQV
                     NQNLEKIRQREGDTH"
     gene            581..697
                     /locus_tag="VQ651_002028"
     CDS             581..697
                     /locus_tag="VQ651_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET2754971.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            690..923
                     /locus_tag="VQ651_002029"
     CDS             690..923
                     /locus_tag="VQ651_002029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754972.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
BASE COUNT          301 a          198 c          225 g          260 t
ORIGIN      
        1 aggaggaaaa ggatgattct cagacatctg ggaattagcc cgaccaatga cttggttgct
       61 aagaaaattt tcagcaaccc agaaatcact tgtcaattta tccgcgatat gctggacttg
      121 ccagccaaaa atgtgactat tttggaggga agtaacattc atgtcttgcc ttccatgccc
      181 tactcagcgc aggattttta taccagtata gacgtcttgg cggagttgga taacggtact
      241 caagtaatta ttgagattca agtccatcat cagaattttt tcatcaatca cttgtgggct
      301 tacctgtgca gtcaggttaa tcaaaatctt gaaaaaattc gtcagcgaga aggtgatact
      361 cactagatct acaaacacat cgctcctgtt tacgtcattg ctatcgtgga tagtaattat
      421 ttctcagatg acctggcttt tcatagcttt agtatgcgcg aagacacaac aggtgaggta
      481 ttggcgatta ccaacaatgg acaggaaaac catctggtta agatggcatt cttggaatta
      541 aaaaatacag agaaaccagc aaagacaagg ttcgcaagcc atggttggag tttttcggca
      601 acaagccctt tacccagcaa ccgcaacgag ccattaccca agcaaatcaa ctgctggact
      661 acaagagctg gtccgaggag gacaggaaaa tgtttagtca actacatatg cgagaagaac
      721 aagtcttgtt agcacaggac tatgccttgg aaactgctag ggctgaaggc cttgaacaag
      781 gactagagcg tgggaaagtt gaaggaaggg cagaaaggaa actttttgcc ttcctagaca
      841 tagtacgcca aggtcttctg acttctgagg ttgccagcca gcaattaggt atgtcagtat
      901 ctgaatttga ggcactgttg taaaatggct ccataatatc catagtgggt aaatccccta
      961 tggatattat ggagcctatt tttg
//
LOCUS       DAVBHS010000223          936 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.223, whole genome shotgun sequence.
ACCESSION   DAVBHS010000223 DAVBHS010000000
VERSION     DAVBHS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.223"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(50..718)
                     /locus_tag="VQ651_002030"
     CDS             complement(50..718)
                     /locus_tag="VQ651_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HET2754973.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVDKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
BASE COUNT          308 a          204 c          152 g          272 t
ORIGIN      
        1 ttttcgaaga gtcttacctc atcaaatttg taaatatcat gagccttctc tagacatcgt
       61 aaccaatatc aaaaaaagct aattctaaag cgactgcttg attccagcgt tgctgaagtt
      121 ctgtcaaatc ttctcgattt ttatcgacac gattgagttc gtcaaccaga aattgaaccc
      181 actctgcaaa gaaaggacct ctgtggagat tgatccattc cgaatgaata tagacttcag
      241 gtaaagccaa atctttagaa ccccagtcta aatagagacc ttctgcaatg accagcatga
      301 ccaaaagatg ggcatagtct gatgaagcca ccgccgaata cattagatcc tgaaaggctt
      361 ttgttacagg gtgcaaagtc acttctagat agtcattctc tgctactttt aactctttaa
      421 aagccttttg gaaataacca tcttcatctg cttcaagaaa gcctagttgc ttggcaaaac
      481 gaagcttgga ttcaagttta tctgcgtgag ctacgcaggc acccagcatg gataagaagg
      541 catcaaagaa gtgataatct tgaatcagat agtcctttaa gaccttattc tcaattgtcc
      601 ccgcaaaaag ttccttaaca aaacgatgat tgattgcagc ctgccaatcc ttctgactgc
      661 tttttaataa ttctccaaca gtcaaacctg gctgaaatgc atagtcttgt gtttccatat
      721 ttacttctcc tctctttact tgttagtaat taataaaaca ccaagaaata tcaagcaaaa
      781 tcgtaattcc acttgatcct tttaaagcac atcgagagca tttgcagaga gctaactaaa
      841 caagcctatc cagtttatat aaacaaaaaa ctccaattac aatcaagaat tagagttgac
      901 ttacaagatt agatcgttca tttcgccata cgaaaa
//
LOCUS       DAVBHS010000224          934 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.224, whole genome shotgun sequence.
ACCESSION   DAVBHS010000224 DAVBHS010000000
VERSION     DAVBHS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.224"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..800)
                     /gene="lytB"
                     /locus_tag="VQ651_002031"
     CDS             complement(<1..800)
                     /gene="lytB"
                     /locus_tag="VQ651_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HET2754974.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGK"
     gene            complement(842..>934)
                     /locus_tag="VQ651_002032"
     CDS             complement(842..>934)
                     /locus_tag="VQ651_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase catalytic subunit"
                     /protein_id="HET2754975.1"
                     /translation="DIIENLPKVMDKYGISSLEELRQEVKESLR"
BASE COUNT          272 a          214 c          126 g          322 t
ORIGIN      
        1 attttcccat caaatttgag ataaaaccaa gattccttat cccaaatcca ttcattggct
       61 gccatgtagc caccagattt tagataataa tagtgaccat tctcgaaaat ccattcttta
      121 tcagcatagt ttccattttc ttttatgtaa aaccaagatt ggtattgttt gtcaaaaagc
      181 caaccttgct gtactttggc accactagca ttcacataag cttgattaat ccactgactt
      241 gttagtagat aaccaccgga tttgaaataa tagtccttcc ctttaatttg gagccattct
      301 ttctctgcgt gctgtccatc tgctttgata taaaaccaag catcgtattg agaatcatag
      361 acccagtctt ctattacttt ggcacctgtt gcaccaacat aggaagttcc tacccaagca
      421 tttcttttca tctttccatc ttggtcaaga taataaaagg ctcccttgtc ttctacccat
      481 tctgatttgg ccatatagcc accagatttg aggtaaaaat agtcgtcacc ttgctttagc
      541 cattcatttt cagcatagtt agcatctgct tttatataga accaagattg ataatgagta
      601 tcaaaaaccc actcattcgc tgcttgactg ccatcttctt tcagatactg ttttccttgc
      661 caagtaccat cactagccat tgcaccctct ggactagcta agaaaaatag cagagctaaa
      721 aaaataaatc ttactttctt cattaacctt cttcctctgt tcttatttat tttattgtac
      781 caaaattctc ctaaattcat attacaaact aataaaaaat caagaacaga ttgattgcag
      841 tttacctcag agactctttt acttcctgac ggagttcttc cagactgcta ataccgtatt
      901 tatccatgac ttttggtaaa ttttcgatga tgtc
//
LOCUS       DAVBHS010000225          902 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.225, whole genome shotgun sequence.
ACCESSION   DAVBHS010000225 DAVBHS010000000
VERSION     DAVBHS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.225"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            122..590
                     /locus_tag="VQ651_002033"
                     /pseudo
     CDS             122..590
                     /locus_tag="VQ651_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          331 a          135 c          166 g          270 t
ORIGIN      
        1 gagaagagga ttgccatctt ctccattttt atgtgcagag ttatagtggc ttgatgctgg
       61 gaaagtacac tgtgactgct aaaacattcc tagaagctgc tttgatttcc ctaatctatt
      121 tatgcaaatt ttatgttatt ttactataac agttgttgct aagccaaata atagtgggga
      181 agttcattta gacgtaagca ttgaagataa tcagggaggt agtgggtata atttcagttc
      241 tgtttcaagt agctcacaaa cagctaaata tgaaggaact ggttataata acaattcatc
      301 attatatata acgattgata aaacgtctga tgcaacagct cttttgaaat taaagttgaa
      361 taatgttgat aatcaacctg ctactgaagt tcctagttca ggaattactg taaaattaaa
      421 tgctaaagat aatgctggaa actggacaag tgcttcgaat aaagacaagt gcttcgaata
      481 aaaaagaaat aacagtaaaa attgtttctg ctaaaccgac atatccagac aaaatcttag
      541 tgaaaaatcc tgataatata aaagatacag aaaaaaatgc cattattgaa aaattgaaag
      601 aggcaaataa aaatcatcca gcaggagctc caacctttgc taaaggtgaa ggagagcatg
      661 caaatgatat tgtagcaact tattcagatg gtacaactta ttatgtaccg ttaaatgatg
      721 tgacaaaata tgcgaggtag tggctgtacc actcacttat tcacctcccc gtgatttgta
      781 gtagtgatag gctttctcac tattattata aaacaaaata aagatcacaa cactttttca
      841 ttctgtgttg tgccttgagt gaaacgaaag gaatgaatta taaatatgaa aagtatagtc
      901 ac
//
LOCUS       DAVBHS010000226          875 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.226, whole genome shotgun sequence.
ACCESSION   DAVBHS010000226 DAVBHS010000000
VERSION     DAVBHS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.226"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(115..330)
                     /locus_tag="VQ651_002034"
     CDS             complement(115..330)
                     /locus_tag="VQ651_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754976.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNHEKFED"
BASE COUNT          286 a          168 c          109 g          312 t
ORIGIN      
        1 aaataaaaag aaaaggctgc atcaaccgtg cagtcttttt gctgtcctca aaatgaaaag
       61 gcagattctc tatctctact acttgaaggt caagaatcca ccgttatcca aagattaatc
      121 ttcaaatttt tcatgatttt tttcatagaa atcaattaag cctagggctg tttcaaatga
      181 aatatttttt acttttgcac gaccctgcgc cagagcaatg atagacattt cacgcgcatt
      241 cgtttcttta gagatacggt agcctgtgat tttcttatca cgaacccagc caacaactga
      301 ttctactttt tcaaagtttg acttagccat gtttttctcc tattttcttc tttataatat
      361 ttaattgtat acgtttttac tacttttgtc aataaaaaaa acatatattc aacgaaaata
      421 attagttcag aattttctta cttccttttt aaatccgata atatataggt tttgctagcc
      481 tctacaccat aaatatttgc taataaaaat actgtattat taccctctta aggtgcaaaa
      541 ttattcaagt tcttaaaaaa tgcttctcta tttccgtaag atagcttatt tcttcgattt
      601 aagaactgaa gatgatagtt ctaaccttta gaaatccaag ccttctctag ctcaaaaact
      661 cctatctttc ttcctcaata aatcatctga gagataggta ttccttatat ccttcaaatt
      721 catcactcct ttggatattg aggtgctagc ctagaagcag acacccgatt atctaaacca
      781 tccttaatag agatgacaag acagataagc tcagttaatt acttatatat ctttttaata
      841 tggtatattt gtcaacaaaa agaaatcaaa tcata
//
LOCUS       DAVBHS010000227          856 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.227, whole genome shotgun sequence.
ACCESSION   DAVBHS010000227 DAVBHS010000000
VERSION     DAVBHS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.227"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(447..845)
                     /locus_tag="VQ651_002035"
     CDS             complement(447..845)
                     /locus_tag="VQ651_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2754977.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT          251 a          168 c          127 g          310 t
ORIGIN      
        1 ccttttgtag tataatggtt ttgcatctct gtgcctttct tgtgtttgtg gttgaacaac
       61 aagtataaca caaaggtgtt ttcttatgcc tacaagagct ttcattattt ccattttctt
      121 ttggatttca ctctattctg aaaaacttgt gtatatttac tgaagctagc aagtcttacc
      181 tgtaaattta atgaaagcaa cacaaaatcc gagaggggaa tctcggatta atgtcaattt
      241 aaaaagatag agagttttta gtttaaataa attgttttaa aatatcaaca acatcacttc
      301 ttttcttaac ctgataagcc ttgattccta attttggggc tacgattata ttgtcctcaa
      361 tatcgtctag aaagacacaa tttctaggtt ataactggta tttatcgata gttactcata
      421 tatatcagtc cacctccata ctcaccttac agattctttt gataataata tctttgccta
      481 atgtagagat agtcttgcaa agaaaaaact tccttgtagc catgtttctg ataaaagtcc
      541 ggtgcctgga actggtaagt attgacaaag gcaaaacaac aatttcgatt cttagcttca
      601 ctttctgcct gttgcaatag ttttgaaccg attccttgcc ctcgcagttc ctcttttaca
      661 aacaaatact cgatttctag ccaatttcca aaagtctctg ctatcaaacc cgcaaggaga
      721 ttgccctttt catcttcgac ataaagatta agtggctcac tttcagcctc ttctcttttt
      781 gaacggttat aaacacgaat cagattccct atttcttgcg atttatgtga ttccttattt
      841 tccaatctaa agtata
//
LOCUS       DAVBHS010000228          830 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.228, whole genome shotgun sequence.
ACCESSION   DAVBHS010000228 DAVBHS010000000
VERSION     DAVBHS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.228"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            21..830
                     /locus_tag="VQ651_002036"
                     /pseudo
     CDS             21..830
                     /locus_tag="VQ651_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
BASE COUNT          266 a          174 c          162 g          228 t
ORIGIN      
        1 aaaagtgtta tactattttt atggaaacag tatacgacaa agcacaaaaa cttaactcaa
       61 aaaacttcaa actattgatt ggtgtcaaaa aggaaacctt tcaactcatg ctagaacacc
      121 tgaattcagc ctatcagatt cagcaccgaa aaggtggacg tccacgtagt ctgcccatgg
      181 aagaccagct cattatgacc ctccgttact tgcgatatta tcccactcag cgtctgctgg
      241 cctttgattt tggcgtcggt gtagctacgg taaatgccat catcacttgg gtggaggata
      301 cacttcgtgc gtcaggtagc tttgatttgg accatttaga agccccgagt gctgctgtgg
      361 ctattgacgt gaccgaaagt ccgattcagc gtccaaaaaa aaccaaagca aaaattattc
      421 tggtaaaaag aaacgacaca ccttaaaaac tcaaattatg ctggatttga cgacacataa
      481 agtctgtcaa atggcctttt ctgacggaca tacgcatgat tttactctct tcaaagaaag
      541 tattggacaa agtttgcctg aaacgacgct tgcctttgtt gacctaggtt atttaggcat
      601 cttgaaattt catgagaata ctttcattcc tgctaaaaat tccaaaaatc gccgcctgag
      661 tgaggatgat aagcagttaa ataaagagat gtcagcgata cgaattgaaa ttgaacattt
      721 taacgctaaa ttcaagacct tccaaatcat gtcagtccct tatcgtaacc gcagaaaacg
      781 tttcgagtta cgggcggaat taatttgtgc catcatcaat tatgaagtga
//
LOCUS       DAVBHS010000229          823 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.229, whole genome shotgun sequence.
ACCESSION   DAVBHS010000229 DAVBHS010000000
VERSION     DAVBHS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.229"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            266..517
                     /locus_tag="VQ651_002037"
     CDS             266..517
                     /locus_tag="VQ651_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HET2754978.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGDQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            505..795
                     /locus_tag="VQ651_002038"
     CDS             505..795
                     /locus_tag="VQ651_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754979.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
BASE COUNT          314 a          105 c          162 g          242 t
ORIGIN      
        1 acctcaaaac ggtgttttga gcaacctgtg gctagtttcc tagtttgcta tttgattttc
       61 attgagtaca aagagcttgg caacaggctc tttttgtatt gcgtttatta agaaaaacaa
      121 aaaacataga tatacattta actaaaaatt attgtatgtt acctccctta attaggaatg
      181 ataagggaat aactagaaag atttgtgaat acaaactatt tctgatatac aaaatataca
      241 gtaataatga atgatgggag atgggatgaa agaatttcaa tttgagagaa agcagcgttt
      301 ttctttgagg aaatatgcaa taggagcttg ttcggtcttg ctaggaacga gtttattttt
      361 tgctggtatg ggtgatcagc ctgtacagga tacagaaacg agttcagcac taatttcaag
      421 tcattatttg gatgagcagg atttatctga aaagctgaaa tctgagttgc aatggtttga
      481 attagaaaac aagcttttga acttatggga gcattaggtt actatgaagg atttgttcct
      541 tatgtttcaa atcaatacaa aaaccaagct gaagaagaag gcaaaccgct atctgataaa
      601 tatattttcg aaaaaatctt aggaaaaaca tatgcagcct tcaaaaaaga tcaaattaat
      661 gagcgtgtcg agaaattagg taagttaaaa cctattacaa taaattacaa cggaaaatca
      721 gaagtaattg atagtaaaga aaaattacaa gagcttatga ataaagccgt taaagacgaa
      781 gtggctcaaa tatagtaaaa tgaaataaga acaggacaaa tcg
//
LOCUS       DAVBHS010000230          806 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.230, whole genome shotgun sequence.
ACCESSION   DAVBHS010000230 DAVBHS010000000
VERSION     DAVBHS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.230"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..>806)
                     /locus_tag="VQ651_002039"
     CDS             complement(<1..>806)
                     /locus_tag="VQ651_002039"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit I is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HET2754980.1"
                     /translation="EKDEVVFPTIQISHTSNSNHGVIEGNDALIYLMNQQQYLEATVE
                     KLQEYLPKENTFKLLQQPPITTSYEELEKFGKANVAEGVLKKVNRQINRVHELERHIQ
                     SNNEEIERLIKWEKLEIIPAHLEQFFFCKGKVGTIPRTVDNRLYNSFLENNIEVQEIF
                     SNDREYGVVVFYQSSCSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENII
                     ASLQDSKNNLIDLQWQIDYILSIYARQISKNNLLSTPHLVALEGWIEETR"
BASE COUNT          249 a          142 c          108 g          307 t
ORIGIN      
        1 acgagtttct tctatccatc cttctagtgc aactagatgc ggagtgctca aaaggttatt
       61 cttagagatt tgacgagcat agatagataa aatatagtca atttgccatt gtaaatctat
      121 caaattattc tttgaatctt gcaacgatgc gataatattc tctttttcag ttattaactg
      181 catgttttct tgatccaaat ctactactcg ctgcttcggt aattcctttc tagaataatc
      241 aaatggttca aataagtatt catcaaaatc aatagagcaa ctggactgat agaaaacaac
      301 aacaccgtac tctctatcat tagaaaatat ttcttgaact tcaatattgt tttctaaaaa
      361 actattgtat aagcgattat ctacagtcct tggaattgtt ccgacttttc ctttacagaa
      421 aaaaaattgt tctaaatgcg caggaataat ttctaatttt tcccatttta ttaatctctc
      481 tatttcctca ttattacttt gaatgtgtct ttctaattca tgaactctgt ttatttgacg
      541 attcactttt ttaagaacac cctcagcaac attagcttta ccaaattttt ctaattcttc
      601 ataagaggtc gttatcggag gttgctgtaa taatttaaac gtgttttctt tcggtaggta
      661 ttcttgtaat ttctctacag ttgcttctaa atattgttgt tgattcatca aataaatcaa
      721 ggcatcattt ccttcaataa ccccatgatt ggaattagaa gtatgagaaa tttgaatagt
      781 tggaaataca acctcatctt tttcta
//
LOCUS       DAVBHS010000231          785 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.231, whole genome shotgun sequence.
ACCESSION   DAVBHS010000231 DAVBHS010000000
VERSION     DAVBHS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.231"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            59..781
                     /locus_tag="VQ651_002040"
     CDS             59..781
                     /locus_tag="VQ651_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2754981.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
BASE COUNT          233 a          137 c          206 g          209 t
ORIGIN      
        1 aaacgaaaag gaggttggtc attgtactag cctccttttg atatgatata atagttctat
       61 gagattagat aaatttttag ttgcctgtgc ggtgggaagt cggactgagg tcaaaaactt
      121 gctcaaggct gggcgcgtga ctgtaaatgg taaaaaagaa aaatcagcta aattgcagat
      181 tgatgaaaaa atagatgaga ttcgctttga tgggcaagtg ttggagtatg aagagtttgt
      241 ctactacatg atgaacaagc ccaaaggagt tatctcagcg actgaggatc ccaagcacag
      301 aaccgttctg gacttgttgg atgacttggc gcggagtaag gaagtttttc cagtaggacg
      361 cttggatatt gacacgcatg gtcttttgct cttgaccaat aatggtcagc tggctcatgt
      421 tcttctttcg cccaagcgtc atgtggacaa gacttatcag gctcaggtca agggaattat
      481 gacccaagaa gatgtggaga catttatcaa gggcattccg ctcaaagact ttacctgtca
      541 gcccgctaga ctggagattc tttccacaga tgcagaaaag aatcagagcc aaatccgtgt
      601 gaccattgca gaagggaagt ttcatcagat caagcgtatg gtgggctact gtggcaagga
      661 agtggtggac ttgcaacgat tgactatggg aactttagta ttagatgaga acctggaacg
      721 aggggaatgg cgtcgcttga ccaaggaaga attagaaatt ctccgcgcta atattattta
      781 attta
//
LOCUS       DAVBHS010000232          778 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.232, whole genome shotgun sequence.
ACCESSION   DAVBHS010000232 DAVBHS010000000
VERSION     DAVBHS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.232"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..>778
                     /locus_tag="VQ651_002041"
     CDS             <1..>778
                     /locus_tag="VQ651_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2754982.1"
                     /translation="NTNTSNNSNTNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVF
                     DPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRIARIIPLRYRSNHWVPDSRPEQP
                     SPQSTPEPSPSLQPAPNPQPAPSNPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDL
                     SAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKTYDLLARIHQDLLDNKGRQ
                     VDFEALDNLLERLKDVSSDKVKLVDDILAFLAPIRHPERLG"
BASE COUNT          254 a          175 c          152 g          197 t
ORIGIN      
        1 caaatactaa cacaagcaac aacagcaaca ctaacagtca agcaagtcaa agtaatgaca
       61 ttgatagtct cttgaaacag ctctacaaac tgcctttgag tcaacgccat gtagaatctg
      121 atggccttgt tttcgaccca gcgcaaatca caagtcgaac cgccagaggt gtagctgtcc
      181 ctcatggtaa ccattaccac tttatccctt atgaacaaat gtctgaattg gaaaaacgaa
      241 ttgctcgtat tattcccctt cgttatcgtt caaaccattg ggtaccagat tcaagaccag
      301 aacaaccaag tccacaatcg actccggaac ctagtccaag tctgcaacct gcaccaaatc
      361 ctcaaccagc tccaagcaat ccaattgatg agaaattggt caaagaagct gttcgaaaag
      421 taggcgatgg ttatgtcttt gaggagaatg gagtttctcg ttatatccca gccaaggatc
      481 tttcagcaga aacagcagca ggcattgata gcaaactggc caagcaggaa agtttatcgc
      541 ataagctagg agctaagaaa actgacctcc catctagtga tagagaattt tacaataaga
      601 cttatgactt actagcaaga attcaccaag atttacttga taataaaggt cgacaagttg
      661 attttgaggc tttggataac ctgttggaac gactcaagga tgtctcaagt gataaagtca
      721 agttagtgga tgatattctt gccttcttag ctccgattcg tcatccagaa cgtttagg
//
LOCUS       DAVBHS010000233          738 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.233, whole genome shotgun sequence.
ACCESSION   DAVBHS010000233 DAVBHS010000000
VERSION     DAVBHS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.233"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            150..686
                     /locus_tag="VQ651_002042"
     CDS             150..686
                     /locus_tag="VQ651_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HET2754983.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
BASE COUNT          175 a          158 c          139 g          266 t
ORIGIN      
        1 attttcgaat caacaaaaaa attatccaac ttgactagtc acgaaaatcg gctgttacag
       61 tttttgttac cttgttttta aaaaatggtt gacatcgact cctatatgag tataatagtt
      121 atcatacatt agaaaagagg ttaactattt tgaaaaaagc tcacgtttat gctatccctg
      181 ctattggggc tgctctcatt gctgctttgg cacaaatcag tcttccaatt ggacctgttc
      241 ccttcactct gcaaaacttt gcaatcggct tgattgctac tgtctttaga ccgagagagg
      301 ctgtactttc tgctggactc tatcttcttc taggtgctat cggtcttcct gtctttgcag
      361 gaggtggagc tggttttcag gctttagttg gccctactgc aggctatctt tggttttatc
      421 tcgtttactc tggacttact tcctctctaa ccaacagcaa gagtggtgtt gttaagattt
      481 ttcttgcaaa cctcttgggt gatgcccttg tctttgtcgg cgggattctc agcttgcatt
      541 tcctagctgg aatggcattt gaaaaagctc ttgctgtggg ggttcttccc tttatcattc
      601 cagaccttgg caaacttctg gctattagtt ttattagccg tcccctactt caacgcctta
      661 aaaatcaggc ttactttact aactaaaaaa ggatatcgag ttatcatgac tcaatatcct
      721 tttcttttat tttgaaaa
//
LOCUS       DAVBHS010000234          716 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.234, whole genome shotgun sequence.
ACCESSION   DAVBHS010000234 DAVBHS010000000
VERSION     DAVBHS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.234"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(1..309)
                     /locus_tag="VQ651_002043"
                     /pseudo
     CDS             complement(1..309)
                     /locus_tag="VQ651_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(385..696)
                     /locus_tag="VQ651_002044"
     CDS             complement(385..696)
                     /locus_tag="VQ651_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2754984.1"
                     /translation="MGLPITRKEIANWHIKASQYYLESLYNLLREKLLEQPLLHADET
                     SYRVLESDSQLTYYWTFLSGKSEKQGITLYHHDQCRSGSVVQEFLGDYSGYVHCDMLR
                     Q"
BASE COUNT          202 a          178 c          128 g          208 t
ORIGIN      
        1 ctgtattcaa ttgcccttcc tagttttgaa cccgataaaa ctgactgacg ccggcaccaa
       61 gcaaagaaat cttccattag gttggagctc ttcttgacgt ttctgtagtc gttcatcagc
      121 tggcaaagcc tcccagtctc tttccaagaa aaataactga tcacaataag ctaaaccttt
      181 agctcctaag gatgatttat ccgcttgctt gggggtcgct tcaaaaaact tccttctcac
      241 atgcgcccaa catccaacaa gtgcagcttc ttccagttga cgataagctt ctagcggttc
      301 gcagtttacc aagcttagcg tcgccttgct cctaaacctt ggactgctat cgcataggca
      361 gaactagagg actaaagtcc taagttactg ccgcaacata tcacaatgca catagccaga
      421 ataatctcct aggaattctt gtactactga accacttcga cactgatcat ggtggtaaag
      481 cgtaatccct tgtttctctg atttacctga caaaaaagtc caatagtaag tcagctgact
      541 atcactttct aagacccgat aagaggtttc atctgcatga agaagaggtt gttctaacaa
      601 cttttctcgt aaaaggttat aaagggactc taaatagtat tgactcgcct tgatatgcca
      661 attagcaatt tccttacgtg tgattggtaa acccatccta gcccaatctt cttctt
//
LOCUS       DAVBHS010000235          687 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.235, whole genome shotgun sequence.
ACCESSION   DAVBHS010000235 DAVBHS010000000
VERSION     DAVBHS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.235"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            151..684
                     /gene="queF"
                     /locus_tag="VQ651_002045"
     CDS             151..684
                     /gene="queF"
                     /locus_tag="VQ651_002045"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HET2754985.1"
                     /translation="MKHQSALVVFSGGQDSTTCLFWTMQHYETVEAVTFAYGQHHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
BASE COUNT          201 a          166 c          127 g          193 t
ORIGIN      
        1 tcctgttttt atttcatttt actatataag tagaatatta cgatgtttgg gatatgccat
       61 cgctaaaaaa aaactcgatt tggtattttt tagttcccta atgggagctt tttgcgtgct
      121 ctgaacattt cccattttgg aaggagtact atgaaacatc aatcagcctt ggtcgtcttt
      181 agtggcggtc aagattcaac aacctgcctt ttctggacca tgcaacacta tgaaacagtc
      241 gaagctgtca cctttgccta cggccaacat catcacctcg aaattcaaat tactagagaa
      301 atcgctaagg aacagggcat tcgtcaccat atcctcgata tgtctctgct gggacaaatc
      361 actgctcagc cagactttgc gacgattcat atttcctaca ttcctgacaa gctctgtgtc
      421 gagtcaaaat ccctcaaact atatctattt agctaccgaa accacggaga tttccacgaa
      481 aactgtatca acaccatcgg gaaagacttg gtcaacttgc tagaccctcg ctatttagaa
      541 gtctggggaa aattcactcc gcgcggtggc atttcaatcg acccctacta taactacggt
      601 aagcaaggaa ctaagtatga gggcttggca gaacaacgcc tcttccaaca cgacctttat
      661 ccagagaaaa ttgacaaccg ctaaagt
//
LOCUS       DAVBHS010000236          673 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.236, whole genome shotgun sequence.
ACCESSION   DAVBHS010000236 DAVBHS010000000
VERSION     DAVBHS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.236"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..244
                     /locus_tag="VQ651_002046"
     CDS             <1..244
                     /locus_tag="VQ651_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HET2754986.1"
                     /translation="IADQQEVLSYLTSVMRGETQEQTLISIGELGQTITDIDVGAKDR
                     IKAAELLGKRHRLWTDKVEADVSGTVVFANESDIPD"
     gene            222..>673
                     /locus_tag="VQ651_002047"
     CDS             222..>673
                     /locus_tag="VQ651_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HET2754987.1"
                     /translation="MSQTYQIKQSDIVIDLPKTVGAGYGQFWRSRNLYRVVKGSRGSK
                     KSKTTALNYVIRLLKYPWANLLVIRRYSNTNKQSTYTDFKWACNVLGVTHLFKFNESL
                     PEITIKATGQKILFRGLDDELKITSITVDVGSLCWAWFEEAYQIETED"
BASE COUNT          212 a          120 c          162 g          179 t
ORIGIN      
        1 aatcgcagac cagcaagaag tccttagtta tctaacctcg gtaatgcgag gagagacgca
       61 agaacagact ttgataagca tcggagaatt gggtcaaacg attacggata ttgatgttgg
      121 agcaaaagac agaatcaagg cagccgaact attaggaaaa cgtcataggc tttggacaga
      181 caaagtagag gcagacgttt ctgggacggt ggtgtttgca aatgagtcag acataccaga
      241 ttaaacaaag tgatattgta atcgacctac ctaagacagt aggagctgga tacggacagt
      301 tctggcgctc aagaaatctt tatcgtgttg taaaaggttc ccgtggttcg aagaagtcca
      361 agacaaccgc tttgaattat gttatccgtc ttttgaagta tccctgggcc aacttgcttg
      421 ttattcgtag atactcgaat accaacaagc aatcaactta tacggatttt aaatgggcgt
      481 gtaatgtgtt gggtgtgact catttgttta aatttaacga gtctttgcct gaaataacca
      541 taaaagcgac tggtcaaaaa atcctattcc gtggtttgga tgatgaactc aaaatcacat
      601 ctatcacggt cgatgtcggc agtctttgtt gggcatggtt tgaggaagca tatcaaattg
      661 agactgaaga caa
//
LOCUS       DAVBHS010000237          631 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.237, whole genome shotgun sequence.
ACCESSION   DAVBHS010000237 DAVBHS010000000
VERSION     DAVBHS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.237"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            191..409
                     /locus_tag="VQ651_002048"
     CDS             191..409
                     /locus_tag="VQ651_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HET2754988.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
BASE COUNT          210 a           74 c          115 g          232 t
ORIGIN      
        1 ggctagtttc ctagtttgct ctttggtttt catttagtat aacagattgt tagaagttgg
       61 tttaaatttc ctaatcagtt tgttcacatt taccttcgat atattatatc ccatagttaa
      121 ggttggtcat acagatgatt atagtcatgg agccgtaaaa cttagtgttt ctttagttga
      181 caaagatgcc atgaaaaaaa tatctgtaac tgtaatagga tattttgaaa taaatataga
      241 tgaaaatatc accgatattc tatacgtaaa tggtactgct attctttatc attatttacg
      301 ttctattgtt tcaatagttt cggcaattga tagcagtgaa gcaatgttgc tacctaccat
      361 taatgtttta gagttactag ataaatctca accttttgaa gaagaataat ttattagctc
      421 actaaattga gggtaaggaa aagtaaaatc agtaagaaaa atgtcttgca ttatacagca
      481 accttttggg aatgagtaga tggattgaat aaaattttat taagagtgga tgatttatct
      541 gtagattatt attggacagt tagtcttgaa gtagtctaag aattaggtta taatcagtag
      601 aatccttgct aataatgagg aggttagttt a
//
LOCUS       DAVBHS010000238          623 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.238, whole genome shotgun sequence.
ACCESSION   DAVBHS010000238 DAVBHS010000000
VERSION     DAVBHS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.238"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..>623)
                     /locus_tag="VQ651_002049"
     CDS             complement(<1..>623)
                     /locus_tag="VQ651_002049"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754989.1"
                     /translation="LAEAEALVDAEAEALVLAEAEALVDADSDAEVLAEAEALVDAEA
                     EALVLAEAEALVLADSDALVDAEAEALVDAEAEALVDAEADALVDAEAEALVDAEAEA
                     LVDAEAEALVLAEADALVDAEAEALVLAEADALVEAEAEALVDAEAEALVLAEADALV
                     DADADALVDADSDAEILAEAEAEALVDAEADALVLAEADALVLAEADA"
BASE COUNT          159 a          204 c          135 g          125 t
ORIGIN      
        1 gcatcagcct cagcaagcac cagtgcgtcg gcttcagcaa gtacaagtgc gtcagcctca
       61 gcatcaacaa gtgcctcagc ttcagcctca gcaagtatct cagcgtctga atctgcatca
      121 accagtgcgt cagcatccgc atcaacgagt gcatcagctt cagcaagcac cagtgcttca
      181 gcttcagcat caacaagtgc ctcagcttcg gcctcaacaa gtgcgtcagc ttcagcaagc
      241 accagtgctt cggcttcagc gtcgacaagt gcgtcagcct cagcaagcac cagtgcctca
      301 gcttcagcat caacaagtgc ttcagcctca gcgtcaacaa gtgcttcagc ttcagcatca
      361 acaagtgcgt cagcttcagc atcaaccagt gcctcagcct cagcgtcgac aagtgcctcg
      421 gcttcagcat cgacaagtgc atctgaatca gcaagcacaa gtgcttcagc ttcagcaagt
      481 accagcgcct cagcctcagc atcaaccagt gcttcggctt cagcaagtac ctcagcgtct
      541 gaatcggcat caacaagtgc ttcagcttca gcaagtacaa gcgcctcagc ttcagcatca
      601 accagtgctt cggcttcagc aag
//
LOCUS       DAVBHS010000239          615 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.239, whole genome shotgun sequence.
ACCESSION   DAVBHS010000239 DAVBHS010000000
VERSION     DAVBHS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.239"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            118..312
                     /locus_tag="VQ651_002050"
     CDS             118..312
                     /locus_tag="VQ651_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2754990.1"
                     /translation="MKIIQQQSATIDSLTNALALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLNLFEEESPSEKDGDVPS"
     gene            299..604
                     /locus_tag="VQ651_002051"
     CDS             299..604
                     /locus_tag="VQ651_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HET2754991.1"
                     /translation="MFPVETEEITYKRKKSKGKRQALLAQFDSEEVHHQVEESICSDC
                     QGDLKEIGATLQRQELVFIPAQLKRIDHIQHAYKCQACSDKNPSDKIVKAPIPKAPL"
BASE COUNT          216 a          123 c          116 g          160 t
ORIGIN      
        1 cttttctatc actccaaaaa taaatccatc agaaagtcgt gatttctatt gaaatgagga
       61 ctttcttttt agtataataa aggtagaaaa caaggagggg aagcccatgg aagaagattg
      121 aaaatcattc aacaacagag tgctacaatt gatagtctca ccaatgcact tgcccttctt
      181 cgtgaacaag tggcttatct gactcaaaag ctctatggaa aatcctctga gaaaagtgtt
      241 tgcccctctg gacaactcaa cctttttgag gaagagtctc catctgagaa agatggagat
      301 gttcccagtt gaaacagagg aaattaccta caaacgtaag aaatctaaag ggaaacgcca
      361 agctcttctt gcccaatttg attcagaaga agttcatcat caagtagaag agagcatttg
      421 ctctgattgt cagggagatc taaaagagat tggagcaacc cttcaacgac aagaattagt
      481 ctttattcct gcgcaattaa aacgcataga tcatatccaa cacgcttaca aatgccaagc
      541 atgcagtgat aaaaatccga gtgataaaat tgtgaaagct cctattccta aagccccttt
      601 ataacgagac tattg
//
LOCUS       DAVBHS010000240          611 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.240, whole genome shotgun sequence.
ACCESSION   DAVBHS010000240 DAVBHS010000000
VERSION     DAVBHS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.240"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(189..>611)
                     /locus_tag="VQ651_002052"
     CDS             complement(189..>611)
                     /locus_tag="VQ651_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2754992.1"
                     /translation="KPNEEKPQTEKPEEDKEHDEVSEPTHPESDEKENHAGLNPSADN
                     LYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIAEAEALLEKVTDSSIRQNAVET
                     LTGLKSSLLLGTKDNNTISAEVDSLLALLKESQPTPIQ"
BASE COUNT          155 a          121 c           93 g          242 t
ORIGIN      
        1 gatgaagttt tttctcaact tcttttactt tattaaatag ttttctaaac cggttattta
       61 accatttaat taaccagtaa atagtttact atttaaaatt acttttgtca agtttctgtt
      121 acgtttttct aaaaaagaga agtaacacga aaggttactt ctccataaaa tatgctccat
      181 tcattttact actgtatagg agtcggttga ctttctttta acaaagccaa gagactatct
      241 acttctgctg aaatagtgtt attatctttc gttccgagaa gaagactact ttttagacca
      301 gtcaatgtct ccacagcatt ttgtctaata ctagaatctg ttactttttc tagcaaggcc
      361 tccgcctctg ctatcttagc gttaataaca gaatgctcta cttgaggaat ttcagcctca
      421 tctgtagtat cttcagcttc ttcctctgtc tcttccgtat cagtgcttgg tttataaaga
      481 ttatctgctg aaggatttaa accagcgtga ttctcttttt catcagattc agggtgagtt
      541 ggctcactta cttcatcatg ttccttatct tcctcaggtt tttctgtctg aggtttctcc
      601 tcgtttggtt t
//
LOCUS       DAVBHS010000241          576 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.241, whole genome shotgun sequence.
ACCESSION   DAVBHS010000241 DAVBHS010000000
VERSION     DAVBHS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.241"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            113..520
                     /locus_tag="VQ651_002053"
     CDS             113..520
                     /locus_tag="VQ651_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2754993.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKIG"
BASE COUNT          197 a          119 c          103 g          157 t
ORIGIN      
        1 cacattgtgt actatttttg gttcatttca ctataataat aacgatacta gataatgagg
       61 aaaatgcaat ggcaaaccca actttcggag aaaaaaagcg aatacagact atattgctcg
      121 ctatggcgta tatgcagtta tccctaatcc tgaacaaaaa caaattgttc ttgttcaaga
      181 accaaatggt gcttggttcc taccatgtgg agaaattgaa gcaggtgaaa atcatcagga
      241 agccctaaag cgtgagttga ttgaagagct tggtttcaca gcagaaattg gtacctatta
      301 cggacaagct gacgaatatt tctattctcg tcatcgtgat acctactact acaatcctgc
      361 ctacctctat gaagcaactt ctttcaaaga agtacaaaag ccactagaaa actttaatca
      421 tattgcctgg ttccctattg acgaggctat ccaaaacctt aaacgtggta gccataaatg
      481 ggccattgaa tcttggaaaa aacagcataa gattggctaa tactcttcga aaataaaatt
      541 caaaccacgt cagcgtcgcc ttaccgtact caagta
//
LOCUS       DAVBHS010000242          572 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.242, whole genome shotgun sequence.
ACCESSION   DAVBHS010000242 DAVBHS010000000
VERSION     DAVBHS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.242"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..>572)
                     /locus_tag="VQ651_002054"
     CDS             complement(<1..>572)
                     /locus_tag="VQ651_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HET2754994.1"
                     /translation="RLPKIMSWDEYAFTKGKMSFIAQDFDNLNIITVLEGRTQAVIRN
                     HFLRYDRAVRCQVKIITMDMFSPYYDLAKQLFPCAKIVLDRFHIIQHLSRAMSRFRVQ
                     IMNQFERKSHEYKAIKRYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEILDKILSYSED
                     LKHHYQIYQLLLFHFQNKDPEKFFGLIEDNL"
BASE COUNT          182 a           99 c          116 g          175 t
ORIGIN      
        1 agattgtcct caatgagtcc gaaaaatttc tcagggtctt tgttctgaaa gtgaaaaagt
       61 aagagttgat agatctgata gtggtgtttc aagtcttctg aatagcttaa aatcttgtca
      121 agaatttctt tatttgttaa gtgcatgcga aaagtagggc gataaaaacg tttatcgctc
      181 aatttacgac tatcctgttg gatgagtttc cagtaacgct tgatagcctt gtattcatga
      241 gattttcgtt caaactgatt cataatttga acacgaaaac gactcatggc acggctgaga
      301 tgttggataa tatggaaacg atctagaacg attttagcac acggaaaaag ctgtttagcc
      361 aagtcatagt aaggactaaa catatccatc gtaatgattt tcacttgaca acgaacggct
      421 ctatcgtagc gaagaaagtg atttcggatg acagcttgtg ttctgccttc aagaacagtg
      481 ataatattaa gattatcaaa atcttgcgca atgaaactca tctttccctt agtgaaggca
      541 tactcatccc aagacataat ctttggaagc cg
//
LOCUS       DAVBHS010000243          563 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.243, whole genome shotgun sequence.
ACCESSION   DAVBHS010000243 DAVBHS010000000
VERSION     DAVBHS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.243"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            207..422
                     /locus_tag="VQ651_002055"
     CDS             207..422
                     /locus_tag="VQ651_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2754995.1"
                     /translation="MILWSFDFANDHAHAFFMDNVEWSHADSYFLSFVSDDVEERYIE
                     NVYLDSLSVKQKFKFIFDFGDEWRFEC"
BASE COUNT          185 a           81 c          127 g          170 t
ORIGIN      
        1 aagaacacct tgcttggcgc agggtgtttc gcgtgaaaca gaagaattat ctggtttcaa
       61 atgctacagt taaaattagt aaaacaagaa atagaagctg aaaagccagc atctgtagaa
      121 gcttggatca tttccgtcaa attaaaaaag gttgctaccg acatatatag attccaaaaa
      181 caaaaacgtt agcggaacta gcagatgtga ttttatggag tttcgatttt gcaaatgatc
      241 atgctcatgc atttttcatg gataatgttg agtggagtca tgcagattct tactttctta
      301 gctttgttag tgacgatgtt gaagaacgtt acatagaaaa tgtctatctg gatagcctaa
      361 gtgtcaaaca aaaatttaag tttattttcg acttcggtga tgaatggcgt tttgaatgct
      421 aagtgctgag agaaatcgag acagaggacg aagaagctta tctcgtacgt tcggttggaa
      481 cgttgccaga acaatatcca gattatgatg gttttgacta tgaagaatgg taaaattgaa
      541 atcagtctgt gtaggcttag tat
//
LOCUS       DAVBHS010000244          560 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.244, whole genome shotgun sequence.
ACCESSION   DAVBHS010000244 DAVBHS010000000
VERSION     DAVBHS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.244"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..>560
                     /locus_tag="VQ651_002056"
     CDS             <1..>560
                     /locus_tag="VQ651_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2754996.1"
                     /translation="YTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHS
                     HWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPL
                     DRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYV
                     EHPNERPHSDNGFGNASDHVQRNKNG"
BASE COUNT          183 a          114 c          126 g          137 t
ORIGIN      
        1 tacactgatg atgagattca agtagccaag ttggcaggca agtacacaac agaagacggt
       61 tatatctttg atcctcgtga tataaccagt gatgaggggg atgcctatgt aactccacat
      121 atgacccata gccactggat taaaaaagat agtttgtctg aagctgagag agcggcagcc
      181 caggcttatg ctaaagagaa aggtttgacc cctccttcga cagaccatca ggattcagga
      241 aatactgagg caaaaggagc agaagctatc tacaaccgcg tgaaagcagc taagaaggtg
      301 ccacttgatc gtatgcctta caatcttcaa tatactgtag aagtcaaaaa cggtagttta
      361 atcatacctc attatgacca ttaccataac atcaaatttg agtggtttga cgaaggcctt
      421 tatgaggcac ctaaggggta tactcttgag gatcttttgg cgactgtcaa gtactatgtc
      481 gaacatccaa acgaacgtcc gcattcagat aatggttttg gtaacgctag cgaccatgtt
      541 caaagaaaca aaaatggtca
//
LOCUS       DAVBHS010000245          533 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.245, whole genome shotgun sequence.
ACCESSION   DAVBHS010000245 DAVBHS010000000
VERSION     DAVBHS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.245"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            56..355
                     /locus_tag="VQ651_002057"
     CDS             56..355
                     /locus_tag="VQ651_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HET2754997.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVNEANIKTLYSEKLIGISIPIK"
BASE COUNT          202 a          103 c           83 g          145 t
ORIGIN      
        1 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
       61 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcaactag aaagtaccga
      121 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      181 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      241 aaccattcaa gaatttttct tgaaacgtaa aattgataag acctctgtta atgaagccaa
      301 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      361 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataaca aaacgcatag
      421 taacaagagt ttctacaccc actattatac gtttttatca ttttgaaatc tgctgctaat
      481 caatgaagaa gcaaagatca aactaggcag ctagttacaa gttacgtaag cgc
//
LOCUS       DAVBHS010000246          514 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.246, whole genome shotgun sequence.
ACCESSION   DAVBHS010000246 DAVBHS010000000
VERSION     DAVBHS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.246"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
BASE COUNT          167 a           85 c          133 g          129 t
ORIGIN      
        1 gggagaggaa gggatagcga aatcgtggct ctacgaacag gaacgtgata gtaaggcgta
       61 tatagcggat aaggaggctt caaactctaa agtccaaaaa ggtagtcgta acctatatgt
      121 gtaaatcacg agagtaattg aattcggact aaggtttgtg tgaaaaagat aaatctttct
      181 agagtctaaa gactctgcgt cagatttcct attttcactg taacctttta acgtcctcat
      241 atcttgtata aacgaggaaa gatgtacgac ttatcccgtg aggtttcatg agcgctgaaa
      301 gcgtagtaac aacgaatcat gagaagtcag ccgagcccat agtagtgagg aaacttccgt
      361 aatggaagtg gagcgaaggg gtgaatactc aaacagtctg gggagagact gtttgaggtc
      421 tgtcgctaga aagagaaaac gacagatcga agtaatccta cttcacttgt gtctgtaaaa
      481 tgagtggtct gatagaactg gactttgaca cgta
//
LOCUS       DAVBHS010000247          513 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.247, whole genome shotgun sequence.
ACCESSION   DAVBHS010000247 DAVBHS010000000
VERSION     DAVBHS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.247"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            152..460
                     /locus_tag="VQ651_002058"
                     /pseudo
     CDS             152..460
                     /locus_tag="VQ651_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
BASE COUNT          204 a           64 c           97 g          148 t
ORIGIN      
        1 tatcgcttac caatatttgc taaactttca gaaaaaaagt tggagtatat gatgctaggt
       61 gactgcgtca tgttagtaaa cgaaatggaa ataactgatc atagagtaga taacttattt
      121 gaaaaaggga aaaatgagat aaaagattca agtagtagat ttagcaatag gacatataaa
      181 agctttagaa aaagtatctg aaaaaacaga tgtttatatc tataacctag gctcgggaga
      241 aggcactagt gtattacaac ttgtaaatac atttgaaagt gttaataaga tccctattcc
      301 ttataaaata gttccaagac gttcaggaga cgttgcaact tattatgcaa atgcagacaa
      361 agcatataag gaattaaatt ggaggacaac aaaatcgatt gaagacatgt gtagagatac
      421 atggaattgg caatcaaaaa atcccaatgg ctataattga tgatactgtc tatatgagtg
      481 ctgtattttg gaaaaattac ttaacctttt aat
//
LOCUS       DAVBHS010000248          508 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.248, whole genome shotgun sequence.
ACCESSION   DAVBHS010000248 DAVBHS010000000
VERSION     DAVBHS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.248"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            29..414
                     /gene="tnpA"
                     /locus_tag="VQ651_002059"
                     /pseudo
     CDS             29..414
                     /gene="tnpA"
                     /locus_tag="VQ651_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148688.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          183 a           73 c          108 g          144 t
ORIGIN      
        1 ccacttgtaa agcgaaagga gaaaaaatat ggcacaaaag gctcatagtt tatctcacac
       61 aaagtggagg tgttctatca cattgtgttc acccctaagt atagacgaaa agtcatctat
      121 aatcaatata gaagtagttt aggcgaaata tttcatcgct tgtgtagtta taaaggtgtt
      181 gaaatgatcg agggtcactt aatgccagac catgtacaca tgttagtcag tattccacta
      241 aggctaagtg tttcgagttt catgggttat ttaaaaggaa aaagtgcact catgatgttt
      301 gacaaacacg ccaatctcaa gtacaagttt gggaatcggc atttctgggt ggaaggttat
      361 tatgtaagtc cagtagggct taatgaagcc acaattaaga aatatagtca agattaaaga
      421 aattatccag tggatgattt cttcacgagt atgaaaattt gagaactagt aaagtatgat
      481 atatagtaaa atgaaataag aacaggac
//
LOCUS       DAVBHS010000249          500 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.249, whole genome shotgun sequence.
ACCESSION   DAVBHS010000249 DAVBHS010000000
VERSION     DAVBHS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.249"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(4..381)
                     /locus_tag="VQ651_002060"
     CDS             complement(4..381)
                     /locus_tag="VQ651_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET2754998.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIALV
                     YAIAIVDSNYFSDDLAFHSFIVK"
BASE COUNT          143 a           94 c           97 g          166 t
ORIGIN      
        1 atttcatttt actataaaac tatgaaaagc taggtcatct gagaagtaat tactatcgac
       61 aattgcgata gcgtatacta gtgcgatttg tttgtagctc tggtgggtat caccttcacg
      121 ttggcgaatt ttttctaggt tttgattaac ctgactgcac agataagccc ataggcgatt
      181 gatgaaaaaa ttctgatgat gaacctgaat ttcgatgata acttggattc cattatctaa
      241 ctccgccaag acgtctatac tagtatagaa atcttgcgct gagtagggca tggaaggcaa
      301 gacgtgaatg ttacttccct ccaaaatcgt cacatttttt gctggcaagt ccagcatatc
      361 gcggataaat tgacaaatca ttagttgggc tgatacctgg atgtctgaga atcctccttt
      421 tcctccttct attatagcac atttttaaat ctaggtttgc tagattctat gcttctatct
      481 atttattcgg aaaagaaagc
//
LOCUS       DAVBHS010000250          476 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.250, whole genome shotgun sequence.
ACCESSION   DAVBHS010000250 DAVBHS010000000
VERSION     DAVBHS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.250"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..>476
                     /locus_tag="VQ651_002061"
     CDS             <1..>476
                     /locus_tag="VQ651_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2754999.1"
                     /translation="EQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEELLMKDPNY
                     QLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHSQHREGGTS
                     TNDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNELSASE"
BASE COUNT          158 a           89 c          106 g          123 t
ORIGIN      
        1 gaacaaatcg tcatcaagat aacagaccaa ggctatgtca cttcacatgg ggaccactat
       61 cattattaca atggtaaggt tccttatgac gctatcatca gtgaagaatt gctgatgaag
      121 gatccaaact accagctaaa ggacgaggat attatcagtg aaatcaaggg tggttatgtg
      181 attaaggtag atggaaaata ctatgtttac cttaaggatg cagctcatgc ggataatgtc
      241 cgtacaaaag aagaaatcaa tcggcaaaaa caagaacata gtcagcatcg tgaaggaggg
      301 acttcaacaa acgatggtgc ggtagccttt gcacgttcac aaggacgcta tactacagat
      361 gatggttata tctttaatgc ttctgatatc attgaggaca cgggtgatgc ttatatcgtt
      421 cctcacggcg accattacca ttacattcct aagaatgagt tatcagctag cgagtt
//
LOCUS       DAVBHS010000251          463 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.251, whole genome shotgun sequence.
ACCESSION   DAVBHS010000251 DAVBHS010000000
VERSION     DAVBHS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.251"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(137..>463)
                     /locus_tag="VQ651_002062"
     CDS             complement(137..>463)
                     /locus_tag="VQ651_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2755000.1"
                     /translation="KPQTEKPEEETPREEKPQSEKPESPKPTEEPEEESPEESEEPQV
                     ETEKVEEKMREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLLA
                     LLKESK"
BASE COUNT           90 a           98 c           77 g          198 t
ORIGIN      
        1 tactattcta gtttcaatct actatatttt gcacattttc atgaaaaaaa tgagaactag
       61 aactcacatt ctgctctcat ttttcgtttt cccgttctcc tatcctgttt ttaggagtta
      121 gaaaatgctg ctacctttac ttactctcct ttaataaagc caatagtttt tcagcttctg
      181 ccataatagt attgttgtcc tgggtgccaa atagtaaatt attttttaat cctgtgagag
      241 tctctttggc attggacttg ataattggat cctggatttt tccaagtaaa tcttcagcct
      301 ctctcatttt ttcttcaacc ttttcagtct cgacctgagg ttcttctgat tcctctggtg
      361 attcttcttc tggttcctct gttggttttg gagactctgg tttctcgctt tgcggtttct
      421 cttctcgagg ggtttcttcc tcaggttttt ctgtctgagg ttt
//
LOCUS       DAVBHS010000252          444 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.252, whole genome shotgun sequence.
ACCESSION   DAVBHS010000252 DAVBHS010000000
VERSION     DAVBHS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.252"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..>444)
                     /locus_tag="VQ651_002063"
     CDS             complement(<1..>444)
                     /locus_tag="VQ651_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2755001.1"
                     /translation="YACKELSADAYFPKLLEGGQLASQPTLSRFLSRTDEETVHSLRC
                     LNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEGVAYNAHYRAHGYHPLYAFEGKTG
                     YCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFRMDSGFATPKL"
BASE COUNT          142 a           86 c          103 g          113 t
ORIGIN      
        1 ataatttggg ggtcgcaaag ccactatcca ttcgaaagag aagttgatta aaccgttcta
       61 aaacaggtgt gataaagctg tctgcctctt cagaacaata acgattacca ggacgaagct
      121 gggcattgaa acaataacct gtcttcccct cgaaagcata aagaggatga tagccatgag
      181 cacgatagtg ggcgttataa gcaacacctt cttgcttgcc ataagttgtg aaatgggtag
      241 aatcgatatc tacaatgagt tggtttagct ggtgaaactg taaaaagaat tcgaccaatt
      301 caaggttgag gcatcgcaaa ctatggactg tttcctcgtc agttctggaa agaaaacggg
      361 ataaggttgg ctgtgaagca agctgccctc cttccaacaa ttttggaaag taggcatcag
      421 ctgacaattc tttacaagca tagt
//
LOCUS       DAVBHS010000253          442 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.253, whole genome shotgun sequence.
ACCESSION   DAVBHS010000253 DAVBHS010000000
VERSION     DAVBHS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.253"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
BASE COUNT          147 a           83 c           78 g          134 t
ORIGIN      
        1 tggtttcgca ggaagtctat tatatatgca cagacatatg aaatcttttt gagacagcag
       61 ttatgatata tttatccatt cacaacatct tctgtactaa tactacaata ttggtacatt
      121 ttgattacac aatcgtttca aaaaatatga tggaagcctg agtattttat agtaattccc
      181 ttcctatcaa ctagatcaac cagttgagtg ttgaatgttg aaacacccta gcctcagcaa
      241 aggactatga tcagcatttc ctagccaata ggacgctcca aatcacctat tctaagtcca
      301 tcactagctt ggtcagtaag atacagactg caaggcgaga ctgacgtggt tgatgagatt
      361 ttggaagagt attacaatca tagattcacc agtagacttg accgcagatt taactatagt
      421 agattgaaac tagaatagta ca
//
LOCUS       DAVBHS010000254          412 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.254, whole genome shotgun sequence.
ACCESSION   DAVBHS010000254 DAVBHS010000000
VERSION     DAVBHS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.254"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            214..>412
                     /locus_tag="VQ651_002064"
     CDS             214..>412
                     /locus_tag="VQ651_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2755002.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDI"
BASE COUNT          125 a           88 c           59 g          140 t
ORIGIN      
        1 cctgcaattg aattcttgct cggctttttg ttttctattt ctttaaaacc ccaattattc
       61 acccctaatc taaaaaccat ccagaatcct tgccttagct tagatcctgt atggtttctt
      121 ttttcaccca atgggtgttt tttactagac aaaaaagaat ttccccttta tggtataagt
      181 gtagaaaaaa acacaaaaag aaaggaaact cacatgaaca gtttaccaaa tcatcacttc
      241 caaaacaagt ctttttacca actatctttc gatggaggtc atttaaccca gtatggtggt
      301 cttatctttt ttcaggaact tttttcccag ttgaaactaa aagagcggat ttctaagtat
      361 ttagtaacga atgaccaacg ccgctactgt cgttattcgg attcagatat cc
//
LOCUS       DAVBHS010000255          405 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.255, whole genome shotgun sequence.
ACCESSION   DAVBHS010000255 DAVBHS010000000
VERSION     DAVBHS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.255"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
BASE COUNT          129 a           71 c           83 g          122 t
ORIGIN      
        1 actaagattt tctattttca ctgtaacctt ttaacgtcct tatatcttgt atacacgagg
       61 aaagatgtac gacttatccc gtgaggtcta tcactataaa gagaaaacga cagatagaag
      121 tgatcctgag tcacggttat ctttaaaata gattgtctga taggacggta tgtataaaac
      181 gcttctgtga actgagagaa gggggagaag ttcttgctaa aatttagttg aacagccgta
      241 ttccgatact tagataagag atctagtctt agctcctact cagttttagg ggataaaaaa
      301 ggggaaatag cgattcgaga aagattatac tcttcgaaaa tctcttcaaa tcacgtcaat
      361 atcgccttgc cgtatgtgta ggatactgac tacgtcagtt ccatc
//
LOCUS       DAVBHS010000256          404 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.256, whole genome shotgun sequence.
ACCESSION   DAVBHS010000256 DAVBHS010000000
VERSION     DAVBHS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.256"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
BASE COUNT          129 a           89 c           59 g          127 t
ORIGIN      
        1 aaaatcctca agatactttc ttctatcctt tagtttataa ggagaatacc tatgaaaaaa
       61 ctgctatttc tatctttgct ctcctaatgt taggagtttg ctgcctgttc ctattcagcc
      121 agcaaagcta taaaaaactc gttcaatact atgctaacga ccagaacctg cccagtagga
      181 taacttatag tgaatatagc gacaaatgag aagccaacta cggtagcact ctaaacatca
      241 cgtctatcaa acaagctaat gacggagttt atgcaaccta tgaagggcaa ttgacacctt
      301 tccaatattg ataaattgat aaccagcctg tcttcatcta gtcatgctgg tttttaagtt
      361 cattttaaat ccttacctat tctccctaac tgtgctatac ttaa
//
LOCUS       DAVBHS010000257          393 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.257, whole genome shotgun sequence.
ACCESSION   DAVBHS010000257 DAVBHS010000000
VERSION     DAVBHS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.257"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(<1..>393)
                     /locus_tag="VQ651_002065"
     CDS             complement(<1..>393)
                     /locus_tag="VQ651_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2755003.1"
                     /translation="YTATIDSESKYLGHKALKIIGLNPSGRDGGNPKVTYPALGQFGK
                     VIPGSTTNQDVTISFYAKANKNGIMLRSRLGNIGYKTGNVTLSTEIKRYVVHIPKGWT
                     NESKQTTNEWLFNFNQEGTVWIWMPKFEI"
BASE COUNT          105 a           87 c           65 g          136 t
ORIGIN      
        1 tatttcaaac ttcggcatcc aaatccaaac ggttccttcc tggttgaaat tgaacaacca
       61 ttcatttgtg gtctgcttgg attcgtttgt ccaacctttt ggaatatgga caacatatcg
      121 cttaatttct gtcgacaatg tcacatttcc agttttatat ccgatattcc ctaatcgaga
      181 tcttagcatt attccatttt tatttgcctt agcataaaaa ctaatggtta catcttgatt
      241 agtcgtactt ccgggaatta ctttcccgaa ttgacccaga gctggataag taaccttggg
      301 attacctcca tcacggccag atggattcag acctataatt ttaagagcct tgtgtccaag
      361 atacttactt tcgctatcga tagtagccgt ata
//
LOCUS       DAVBHS010000258          386 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.258, whole genome shotgun sequence.
ACCESSION   DAVBHS010000258 DAVBHS010000000
VERSION     DAVBHS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.258"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            147..281
                     /gene="rpmH"
                     /locus_tag="VQ651_002066"
     CDS             147..281
                     /gene="rpmH"
                     /locus_tag="VQ651_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET2755004.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
BASE COUNT          132 a           66 c           83 g          105 t
ORIGIN      
        1 agggcttgca ttcgaaaaag ctggaaacat ttgcctagag ctattgacaa aagtgaaaga
       61 atcagataga atagtaaagt atgtttagta actgatcact ttatagccgg ctaaacgaat
      121 acaaaatcta tgaggaggta ttcatcgtga aacgtactta tcaaccaagt aaacttcgtc
      181 gtgcgcgcaa acacggattc cgtaaccgta tgtcaactaa aaatggtcgt cgcgtattgg
      241 cagctcgtcg tcgtaaagga cgcaaagttt tggctgcata atccaaacga attctatcaa
      301 aaatcagtag gaactcgagt ctactgattt ttatttttgt aaaaaagttc agtagatgca
      361 aatggattcg gaagcgatgt tatagt
//
LOCUS       DAVBHS010000259          332 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.259, whole genome shotgun sequence.
ACCESSION   DAVBHS010000259 DAVBHS010000000
VERSION     DAVBHS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.259"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(1..332)
                     /locus_tag="VQ651_002067"
                     /pseudo
     CDS             complement(1..332)
                     /locus_tag="VQ651_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          103 a           59 c           72 g           98 t
ORIGIN      
        1 aaatttctct ggttccttat tctggaagtg aaaaagcaag agctgataga gctgatagtg
       61 gtgtttcaag tcttccgaat agctcaaaag cttgtctaaa atctctttat tggttaaatg
      121 catacgaaaa gtaggacgat aaaaacgctt atcactcagt ttacggctat cctgttgtat
      181 gagcttccag tagcgcttga tagccttgta ttcatgggat tttcgatgaa actgattcat
      241 gatttaaaca cgcacacgac tcatagcacg gctaagatgt tgcacaatgt gaaagcgatc
      301 aagaacgatt ttagcattcg ggagtgaaac ag
//
LOCUS       DAVBHS010000260          319 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.260, whole genome shotgun sequence.
ACCESSION   DAVBHS010000260 DAVBHS010000000
VERSION     DAVBHS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.260"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
BASE COUNT          109 a           62 c           63 g           85 t
ORIGIN      
        1 atgacaagcc gacgctgacg tggtttgaag aaatttttga agagtatcat tcaccatttc
       61 acaactaaat cgaagacttt ctgccgtaat gaaaacacct gaaacagctt ggtttcagct
      121 gtccggaaac tttaagactt aggttcaaag tttaggtatg gaacttcgaa ggaggtcgct
      181 accgtccgtc attacttagg aaagtcttaa aaaatctata aagtaaaaag agcccgacag
      241 ctctttttgt ttatactcaa tgaaaatcaa aaagcaaact aggaaactag ccacgggctg
      301 ctcaaaacac tgttttgag
//
LOCUS       DAVBHS010000261          317 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.261, whole genome shotgun sequence.
ACCESSION   DAVBHS010000261 DAVBHS010000000
VERSION     DAVBHS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.261"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..>317
                     /locus_tag="VQ651_002068"
     CDS             <1..>317
                     /locus_tag="VQ651_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HET2755005.1"
                     /translation="RAYEYIYAKEGMAELDSTSIDPENTFMVYDDSIERKPLFAVYYY
                     EVKDDTKDTTKHQAEVFTENLHYHMVLRSTDSGTTQSEEATPHNLGQIPIIEYRNNHF
                     AIG"
BASE COUNT           99 a           65 c           72 g           81 t
ORIGIN      
        1 tcgtgcttac gagtacatct atgctaaaga gggtatggct gagttggatt caactagtat
       61 tgatccggag aatactttca tggtctacga tgatagtatt gagcggaagc ctttgtttgc
      121 ggtctattac tatgaagtaa aagacgatac gaaagacact accaagcacc aggctgaggt
      181 ctttaccgaa aatctgcact atcacatggt gctgagaagt acagattcag gaacaactca
      241 gagcgaggag gcaacacctc acaaccttgg tcaaatccca attatcgaat atcgcaacaa
      301 tcactttgca attggtg
//
LOCUS       DAVBHS010000262          315 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.262, whole genome shotgun sequence.
ACCESSION   DAVBHS010000262 DAVBHS010000000
VERSION     DAVBHS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.262"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            24..315
                     /locus_tag="VQ651_002069"
                     /pseudo
     CDS             24..315
                     /locus_tag="VQ651_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          119 a           54 c           54 g           88 t
ORIGIN      
        1 aaaaagtatt atataatagt aatatgaaat caattaaaga agaaatccaa accatcaaaa
       61 cacttttaaa agactctcgt acagctaaat atcataaacg tcttcaaatc gttctatttc
      121 gtctgatatg ggcaaatctt ataaagagat tatagaactt ttagactgta atcaaacaat
      181 gatttggcga aatgtaaaaa aatatgagga gttcggactc gactctctcc ttcaagaaac
      241 acgtggtggt cgtaaccatg catatatgac agttgaggaa gagaaagcct ttcttgcccg
      301 ccatttgaag gctgc
//
LOCUS       DAVBHS010000263          297 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.263, whole genome shotgun sequence.
ACCESSION   DAVBHS010000263 DAVBHS010000000
VERSION     DAVBHS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.263"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            1..297
                     /locus_tag="VQ651_002070"
                     /pseudo
     CDS             1..297
                     /locus_tag="VQ651_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT           93 a           60 c           64 g           80 t
ORIGIN      
        1 aggtcgttcc tacacacgtg atgccttcta tcaactgttg aagcgccatg gttggcgaaa
       61 tattatgcca cgtccagaac atcctaagaa agcagacgct caaaccattg tcgcgtctaa
      121 aaataaaatc tcaattcaag aagacaagaa agcgttttaa atatagtaga cgttttcgta
      181 aggttcgctt gatgtaccaa gatgaggctg gtttcggtag aatcagtaaa ctgggatctt
      241 gttgggctcc aataggagta ggtccacata tccatagtca ctatatacga gaatttc
//
LOCUS       DAVBHS010000264          292 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.264, whole genome shotgun sequence.
ACCESSION   DAVBHS010000264 DAVBHS010000000
VERSION     DAVBHS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.264"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            51..>292
                     /locus_tag="VQ651_002071"
     rRNA            51..>292
                     /locus_tag="VQ651_002071"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           90 a           54 c           81 g           67 t
ORIGIN      
        1 gaaaaaaaca ataaatctgt cagtgacaga aatgagtgag aactcaaact tttaatgaga
       61 gtttgatcct ggctcaggac gaacgctggc ggcgtgccta atacatgcaa gtagaacgct
      121 gaaggaggag cttgcttctc tggatgagtt gcgaacgggt gagtaacgcg taggtaacct
      181 gcctggtagc gggggataac tattggaaac gatagctaat accgcataag agtagatgtt
      241 gcatgacatt tgcttaaaag gtgcacttgc atcactacca gatggacctg cg
//
LOCUS       DAVBHS010000265          287 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.265, whole genome shotgun sequence.
ACCESSION   DAVBHS010000265 DAVBHS010000000
VERSION     DAVBHS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.265"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            33..>287
                     /locus_tag="VQ651_002072"
     CDS             33..>287
                     /locus_tag="VQ651_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2755006.1"
                     /translation="MFDKEQKRGYKQSARNLANWYYNDKEDSYTHPDGWYYRFHHTKH
                     QKTQTDFKQEIKVYYADEPESAPQKGLYMNEGYQNLKAKEC"
BASE COUNT          114 a           53 c           57 g           63 t
ORIGIN      
        1 aaaaggaggt aaaccatctg attaaatatg ccatgtttga taaggaacag aagagaggct
       61 ataaacagtc agctagaaac ttagcgaatt ggtactataa tgacaaggag gatagctata
      121 cacatcctga tggctggtat tatcgttttc accataccaa acatcagaaa acacagacag
      181 actttaaaca agaaatcaag gtttactacg ccgacgaacc tgaatcagcc cctcaaaagg
      241 gactgtatat gaacgaaggc tatcaaaact tgaaagctaa agaatgt
//
LOCUS       DAVBHS010000266          279 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.266, whole genome shotgun sequence.
ACCESSION   DAVBHS010000266 DAVBHS010000000
VERSION     DAVBHS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.266"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
BASE COUNT           97 a           51 c           49 g           82 t
ORIGIN      
        1 gattttactg tcctgatcga tttgttctgt tcttgtttca ttttactata tgttcgtaaa
       61 tgaaagttct ataatgaagt tagccaccca gccctcctaa aaattagatg gttcaactag
      121 ctgatagctc ttgtaggcat aagaaaacac ctctgtgtta tacttgttgt tcaaccacaa
      181 acacaagaaa ggcacagaaa tgcaagacaa ttatactaca aaaggtaaac atttgacaat
      241 cgatagccgt cgcttaatcg aaagatggaa aaaagaagg
//
LOCUS       DAVBHS010000267          277 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.267, whole genome shotgun sequence.
ACCESSION   DAVBHS010000267 DAVBHS010000000
VERSION     DAVBHS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.267"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..>277
                     /locus_tag="VQ651_002073"
     CDS             <1..>277
                     /locus_tag="VQ651_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2755007.1"
                     /translation="QRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAM
                     QGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQG"
BASE COUNT          126 a           37 c           47 g           67 t
ORIGIN      
        1 aacaacgcat aatagaagca atcgaatcag ctttagaaaa agtagatgaa tatgctgaaa
       61 gttataatag actagaacag ctagataaag aatttccaga taaactaaaa aaatctattc
      121 ttcaatatgc tatgcaagga aaattagttg aacaagaccc aaatgatgaa tcagtcgaag
      181 ttttacttga aaaaatacga gcagaaaaac aaaaactctt tgaagaaggc aagattaaaa
      241 agaaagattt ggacatttct attgtttccc aaggaga
//
LOCUS       DAVBHS010000268          275 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.268, whole genome shotgun sequence.
ACCESSION   DAVBHS010000268 DAVBHS010000000
VERSION     DAVBHS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.268"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            complement(1..275)
                     /locus_tag="VQ651_002074"
                     /pseudo
     CDS             complement(1..275)
                     /locus_tag="VQ651_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           88 a           45 c           57 g           85 t
ORIGIN      
        1 tgaaaaagca agagctgata gagttgatag tggtgtttca agtcttccga ataactcaaa
       61 agcttgttta aaatctcttt attggttcaa tgcatacgaa aagtaggacg ataaaaatgt
      121 ttatcactca gtttacgact atcctgttga atgagtttcc agtagcgctt gatagccttg
      181 tattcatggg attttcgatg aaactgattt atgatttgaa cacgcacacg actcatagca
      241 cggctaagat gttgtacaat gtgaaagcga tccaa
//
LOCUS       DAVBHS010000269          268 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain RG2057
            SAMEA1678044-rid20172783.denovo.269, whole genome shotgun sequence.
ACCESSION   DAVBHS010000269 DAVBHS010000000
VERSION     DAVBHS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1678044
            Sequence Read Archive: ERR223018
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980121.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980121.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/12/2023 23:26:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,042
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 52 of 99
            Pseudo Genes (incomplete)         :: 39 of 99
            Pseudo Genes (internal stop)      :: 26 of 99
            Pseudo Genes (multiple problems)  :: 16 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1678044-rid20172783.denovo.269"
                     /strain="RG2057"
                     /db_xref="taxon:1313"
     gene            <1..186
                     /locus_tag="VQ651_002075"
     CDS             <1..186
                     /locus_tag="VQ651_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2755008.1"
                     /translation="IFTKQIRHWILSDQVISGKRKLFFREDTPKEILDLYENIKSKLD
                     FAYQEVHSNNGLKKYEK"
BASE COUNT          100 a           33 c           44 g           91 t
ORIGIN      
        1 atttttacaa aacaaattcg tcattggatt cttagtgatc aagttatttc agggaaaaga
       61 aaacttttct tccgggaaga tactccaaaa gagattttag atttgtacga aaacattaaa
      121 tctaaacttg attttgctta ccaagaagtt cattctaata atgggttgaa aaaatatgaa
      181 aaatagaata attgatgttt ttgaagtagt aaatcgcctc cttgtaataa ctgttgaaaa
      241 tcctgatttc gaagacttga gagttaat
//
